LOCUS       DAOSFP010000001        32500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_1070_length_32500_cov_4.126892, whole genome shotgun sequence.
ACCESSION   DAOSFP010000001 DAOSFP010000000
VERSION     DAOSFP010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32500
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_32500_cov_4.126892"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1294)
                     /locus_tag="PLI16_00005"
     CDS             complement(167..1294)
                     /locus_tag="PLI16_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOH82969.1"
                     /translation="MNIAVNTRLLLDNRLEGIGWFTFETLRRIVTAHPEHTFYFIFDR
                     PYHKKFIFSDNVVPVVVGPQARHPLLFYLWFEFSVPRVLKRIKADVFLSPDGYGSLRC
                     RVQQLLVMHDLNFEHYPKDLPWIISRYYRYFFPRFARKATRIATVSEFSKQDISATYG
                     IAADYIDVVYNGVNENFAPIAAEQKNIIKQRYTKGCDYFVYIGALLPRKNIKNLFLAY
                     DMFRKQHSGNIKLLMAGARMWWTPEMETVLGGLEYKNDILFTGRLDSGELNDVLGAAL
                     ALTYVSYFEGFGIPILEAFRCDVPVITSNVTSMPEVAGGAALLTNPQQPESICSAMTQ
                     IAGDDNLRQSLIAKGRQRHTAFSWKKSAALLWQSIEKIINQ"
     gene            complement(1306..2535)
                     /locus_tag="PLI16_00010"
     CDS             complement(1306..2535)
                     /locus_tag="PLI16_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH82970.1"
                     /translation="MDIQAIKQRFGIIGNSPQLNLAIDIARQVAPTDLSVLITGESGA
                     GKEFFPQIIHQLSARKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAYEARKGYFEV
                     VNGGTIFLDEIADMPLPTQARLLRVLESGEFIKVGSSKVLKTDVRVIAATNVNVTEAI
                     HQNRFREDLYYRLNSVPITIPPLRERKDDILLLFRKFTTDFAEKYKMPPIVLDEESRA
                     QMLAYRWPGNIRQLKNIAEQISIIEKQRNINAATLSGYLPHSSESKLPVLLRQPADKA
                     EISERDLLYRVLFEMRKDITDLKHLVADILKEGNVPVNAQDIKNQFLQNYHQDYETVT
                     TQEPAVKINKPETYIAEPIQDPDVYEESLSLQDREIEMIKKALDKYHGKRKNAAKELG
                     ISERTLYRKIKEYNIDN"
     gene            complement(2781..4418)
                     /gene="groL"
                     /locus_tag="PLI16_00015"
     CDS             complement(2781..4418)
                     /gene="groL"
                     /locus_tag="PLI16_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOH82971.1"
                     /translation="MAKEIIFDIKAREALKKGVDELSNAVKITLGPKGRNVIIDKKYG
                     APQITKDGVTVAKEIELKDGVENMGAQLVKEVASKTADLAGDGTTTATVLAQAIITTG
                     LKNVTAGANPMDLKRGIDKAVQNVIENLKKQSVSVGDSFDKIEQVATISANNENAIGK
                     LIAEAMAKVKKEGVITIEEAKGTETVVKVVEGMQFDRGYISPYFVTDTDKMETVFENP
                     FILIYDKKISVMKDFLPILEKEVQTGRPLLIISEDVEAEALATLVVNKLRGSLKIAAV
                     KAPGFGDRRKAMLEDIAILTGGTLISEETGYKLEDAELSYLGQAEKVVVDKDNTTIVS
                     GKGSHEQIKARVAQIKTQIENTTSDYDREKLQERLAKLSGGVAVIQVGAASEVEMKEK
                     KDRYDDALNATRAAVEEGIVPGGGIAYIRTIDALSKLKGANEDEEIGIAIIKRALEEP
                     LRQIVANAGLEGSVIIQKVKEGKGDFGFNALTETYENLYKSGVIDPTKVTRVALENAA
                     SIAGMFLTTECVISDIKEETPAAPMMNPGMGGMGGMM"
     gene            complement(4458..4736)
                     /locus_tag="PLI16_00020"
     CDS             complement(4458..4736)
                     /locus_tag="PLI16_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOH82972.1"
                     /translation="MSKKFGTPMKDRVLVEPAAAEEKTAGGIIIPDTAKEKPQKGTIV
                     AVGKGKKDEPMTVKEGDVVLYGKYSGTEVVIDGKTFLIMREDDIYMVL"
     gene            complement(4923..5264)
                     /gene="secG"
                     /locus_tag="PLI16_00025"
     CDS             complement(4923..5264)
                     /gene="secG"
                     /locus_tag="PLI16_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOH82973.1"
                     /translation="MYVVISVLIFIVCVLLVLIVLVQNSKGGGLASNFQSSNQFMGVR
                     KTADFLEKATWTLAIALLVLTLSSVFVIPRANKDTAVESELREQVENNQAVDYTPAPE
                     NPNAPAAPKNQ"
     gene            complement(5292..6383)
                     /locus_tag="PLI16_00030"
     CDS             complement(5292..6383)
                     /locus_tag="PLI16_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH82974.1"
                     /translation="MNKNDFTGYLSRPENIRNSKAGELTFLLEKFPYFQSAHLVYAAY
                     LSSQDDIAFHDQLKLTAAHVNDRSVLYWLIYNQTHVSTTLPGTGLETPVEVPETPFSI
                     ARQVATETAATTMQERLPEVQQQPDLPSGMVSQTQELPVTEEKTSQEQVQDKMESAQE
                     LVPQQNAEVEAEKAEELSVSTQTVEITGPGIDEEEQRLSQEVAQGAMETVVENETSEE
                     EMPTEEPAKIITPASHNQPAGFLLNIMAKRILSEDSGEKEALPTSVTEKKETVLPEKN
                     TELIEKFIKEEPRISAPKRDFFNPVNMAENSSVDKEEIVSETLAKIYISQGLLQKALK
                     IYQKLYLLNPEKSAYFAAQIENLESKINN"
     gene            complement(6390..6902)
                     /locus_tag="PLI16_00035"
     CDS             complement(6390..6902)
                     /locus_tag="PLI16_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOH82975.1"
                     /translation="MNPKGLFYALCMAVALLMTSCTMKYSFTGASIPPEVKTISIHYL
                     KNTALLVKPTLSQQLTDELRNRFSTQTSLTPVNQNGDFDISGEITNYATSPVAIQGNQ
                     TAAMNRLTVTISIRFVNKYDEKQNYESSFSRYVDYPSTQALASVENQILEEIISYLVD
                     DVFNKTAVNW"
     gene            complement(6990..9740)
                     /locus_tag="PLI16_00040"
     CDS             complement(6990..9740)
                     /locus_tag="PLI16_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH82976.1"
                     /translation="MKYQRSDRRIRTRRYFVTGWVVLAIVALLVVGYSYLKRLKDPVL
                     PAIKAIPESTVCLMEFKNTLSLWDRLHEKNNLWKELQCLSFVSDLNRGLQALDSLANT
                     DDDVADILTQNPLYVAWVPDRAVYRYLFAITLNGPHREKLVDEFMRAHFSQGNTILSH
                     QFLKNEIFEIYKAGKLICAYTVYQGVLIIGETSGVVESSLSTLLTKISMENDADFMRL
                     NAAAGKNVEANIYIRNTYLDNLVSGYFTGMQYRKIKFLSLTGDMVGLDLTVKNDELLF
                     AGYALSGKKDQLLNRVFSGQSPQPIELTDICPANTAVLTFWGVSNANAYVQNYTDFVK
                     THFHRHAFNELKAYYDTTYEINISEKFLNQIGNQFACVVTENPNIENPFNYYGVFKAR
                     HMGNFKQTLADMSVVPEEQFLARRDSFAIRKFLPVNFLKDFFGTMFDALDTAYYTTIR
                     DYVVIGQSPLSLDLFVSGYLSGKTLQKNLNYIDFSDNVADEANFCLYTNIRKSFGLWL
                     PLFNKKLQSDFLRNETAMKNFQALALQFTSDNSKFFLNAYIKHNSDYVEENPAVWEYH
                     ADTSISGKISVIIDPRDSTKKIVFFDASNNIYLLGRNGQLIWKKQIREAPLSKVFVTS
                     LKKDNKYYLIFNTKNLIYTFDLLGNKPDYSPLKLPYPASTAITLINYRGNDDYRIIIP
                     CTNQKIYNYLLNGKPTPGWNNYRTEAILQQPVEYVRFQDKDMMIAVDKNGKVYFLSRN
                     GTLLIKNKQAFIKGKNSRFYIHKDGKKQYLLTTDRKGKMIFISGNGKIDVVTLQPFSE
                     NHYFLVGDFDMDKWNDFIFFDMGKVIVYNHVKKKIFEARLKGNPAGKPQYAGLSRSKF
                     QLVFPDSACSHLVLLNNKGMEELLNYPLGSPEIEITSLFGKNSRSILVSDSNTLLNYI
                     IN"
     gene            complement(9797..10393)
                     /locus_tag="PLI16_00045"
     CDS             complement(9797..10393)
                     /locus_tag="PLI16_00045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOH82977.1"
                     /translation="MLKSYFRKNTIEAGCDEAGRGCLAGPVFAAAVILPKDYKNNALN
                     DSKQLSEKHREILRVEIEKEAVAWAVAQLDNLRIDEINILNASIMSMHLAIRQLSLTP
                     ELLLIDGNRFKPYEKIPHECIVKGDGKYLSIAAASVLAKTHRDEFMITLHDEFPEYNW
                     AQNKGYPTRDHKDAINLYGITPYHRKSFNLNEQLKLIF"
     gene            complement(10407..11702)
                     /locus_tag="PLI16_00050"
     CDS             complement(10407..11702)
                     /locus_tag="PLI16_00050"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH82978.1"
                     /translation="MEQQAKKRSGFRSFPKNFWTVITMEFFERGSYYGVMSILSVYLV
                     MSTQDGGLGFSKESVGVIKSTITPLLYLLPIISGAIADRYGYRKTLFFAFIVMSCGYF
                     LTSQFTDYPLVFASLLLMVLGAGFFKPIISGTIARSTDEKNSSLGFGIFYWSINLGAF
                     LFPLILIPYLKSYSYNYIFIMASVITGSLLLLNLLVYREPARPASSKSLLTILKDALL
                     VIKDYRFILMIVIYSGFWILYFQMFDTVLWYLSEHMDMTPVNDFVNSVLSLFVANSQW
                     KFEAEHVTVINAGTIILLQILISNIVKHTKALPTMIVGILLGTIGMAILGTSSNAWVF
                     IAGLIIFSLGEMTAHPKFISYVGLIAPADKKALYLGYSFLYGVIGSGIGGVLGANLYV
                     YYIEKLNNPSALWFTFSLIGVITIIGLLLYNRFLAPRKV"
     gene            complement(11767..12780)
                     /locus_tag="PLI16_00055"
     CDS             complement(11767..12780)
                     /locus_tag="PLI16_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOH82979.1"
                     /translation="MFDPTHFHPMIVHFPVALIMIGFLADVLSLFFTKKEPCLSKVGF
                     YLMILGTLGAFAAYFTGEFFTRELTGEAGELKERHEVFAKITMFVMLAASLLRIYLVW
                     KKKSNSGLKWVVFFLYLIATCTVGFTGLLGGTLVYNHMVGLESASVEQTVAVTNKTVD
                     NLKTALQGETTASEKYAAFAKKAGEEGLPRIAALFDAASKAESVHAANHRNVLATLGH
                     KVDVQPETFTVNNTLENLLAAYEGEKHETESMYPGFIEQAQTDNNNDAIKSFGWANDT
                     EMKHMELYQAAADALKSGKEKTLPAEYYVCPKCGFTYSNEDIEDYCGLCGTEKSKFFV
                     FGK"
     gene            complement(12803..14953)
                     /locus_tag="PLI16_00060"
     CDS             complement(12803..14953)
                     /locus_tag="PLI16_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOH82980.1"
                     /translation="MKKLFFMCLLAILCAQNKGRADEGMWLPMFIDRLNYTDMQKMGL
                     KLTPEEIYSVNHASIKDAIILFGRGCTGEIISSQGLILTNHHCGYGAIQSKSTVEKDY
                     LTDGFWAMNMKEELPIGDLSVKFLVRIEDVTQKVLSGLQANLTEKQRTAKVEELCAKI
                     EKEATKDNGYDAVVKSFFEGNEYYLIVYQTYPDVRLVGAPPSSLGRYGADTDNWMWPR
                     HKADFCLFRVYTAPDGSPAPYSDKNIPMKPKHSLPVSIKGVKNGDFTMIMGYPGTTER
                     YLTSYGIEMYANQSYPARIKAREMKLAIMREDMDASPEVRIKYSSKYASISNYWKNFI
                     GTVKALNRLKVADKKRHLESQFEAWVMADNGRKEKYGSVLITIKEAYAELRKTNQART
                     YFSESIMGCEAMVFSRRFKSMQPLLKEKPVNADGLKAKTSELRTAAEKFYKDYNKPTD
                     VKLTAAMLNMFYEEVPKSQQPAYFLKLVAKYKYDFNKLSAYLFSKSIFGDLSTVNGFL
                     DAPSAKVLESDALLMLINAFAEQNNVVSEAWAVQNDKLSLGKRLLVAGIHEMLPDQKF
                     YPDANQTMRLTYGKVQDYYPADAVHYNYYTTLEGVMQKEDPDNWEFTVPRRLKELYNS
                     KDYGVYGSNGEMIVNFISNTDITGGNSGSPVLDAQGNLIGLAFDGNWEAMSGNILFEP
                     ELQRTINVDIRYVLFIIDKYAGAKNLIAEMKIIQ"
     gene            15050..16069
                     /locus_tag="PLI16_00065"
     CDS             15050..16069
                     /locus_tag="PLI16_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH82981.1"
                     /translation="MIEKGYKYFLLIVLILFSSCHRDLTDPFIPKDKKFDTQFKFSTR
                     KNRTVEKHSSFAHSKGGFNDSFGFSRRGGHRGSGGKDSFSFKPSKKTGGGFGKDSFAS
                     PRGKRTGGGFGKDSFSSKRGKKTGGGFSKDSYSASGKSRFGKAAPRFLSKRGGKKDSY
                     SLKHKNVSGMFRFSKTKNSIVSKQKFLVFSNHKRINESGSFRAKSKKVSGQFRYSGHK
                     RMVSKKRMVFSGAKRLRASESFNVRVKRHPRVFGFNAEKKRVSPIVSLQRHKDTDAGS
                     FGHGRKNANFSRQYVFSKKQKRVKKRDSIWWDMNQSFHKKKRKKKLLELELFDPKMKR
                     EMKLK"
     gene            complement(16073..17872)
                     /locus_tag="PLI16_00070"
     CDS             complement(16073..17872)
                     /locus_tag="PLI16_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HOH82982.1"
                     /translation="MKSFKIISFIFLFLVLFTTRTSAQVLPETSRVDWSSAGSSFVFP
                     ITAPQVSILSFGGVADGATDNTVALTNAMASFGGHPGIVFFPAGLYLFSSVVSIPDSV
                     WLTGAGPDSTFIKIGHSSTGFSFSGTATNIFTDVISGFQKESHKITVANPALFQPGNY
                     CEIRQDNGSWDVEPATWATYSVGQMILVDSIIGDTLVLADGLHITFDTSLNLQIQKIN
                     PRKASGISCINVARTDSSTVNSGNNFGFIYAVNCVIRGVESNKSQGSHCMIRLSSHIS
                     LEGCYFHDAFLYDGSSTKGYGITCTNHATLCLIQNNIFNHLRHAMMTKQGANGNVFAY
                     NYSLNPFRNGSMEYPQDYCGDISLHGHYSFANLFEGNIVQTIMIDETWGPSGPYNTFF
                     RNRAERYGIIMAATLTNNQNFVGNEITGTDYTFPFQWGAYTITGSGNIQYGNNDNGTI
                     VPNGTNVLNDNSYYLETAPAFWGVSMAWPDIGTPNLLNVGTSPAKDRFYSMELTDCSL
                     HSLITKTTITTPEDILIFPNPTTSRLFITLPGENNFCRVQIKDLTGKCLIDRINNAAF
                     YEAGTDISALKPGIYVVCVKACNNQYTSKIVKF"
     gene            17939..18949
                     /locus_tag="PLI16_00075"
     CDS             17939..18949
                     /locus_tag="PLI16_00075"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH82983.1"
                     /translation="MILVTGGTGFLGSHTLMQLCEQGKKVRALKRSASDTAFVRQLFS
                     FYHKEHLFDSIEWVEGDILDIHSLLPAMKDCEQLYHCAASVGFSNKDTATLTKNNIQG
                     TQNVVNAALETGIKKMCHVSSIAVLGKEALITDDTFWDAGERHSAYAESKYKAEQEVW
                     RGVAEGMDVVVVRPSVIIGPWKQQGGIGVLFRKIEKGLRFYTPGSTAYVDVNDVARAM
                     ITLTDSLVKNDSFIVSAENLTYRELIGMIANIAGKPAPRWCAGRFLSGLAWRLFVLKD
                     FFTRSDSGFNEITAEISQTTSLYSNKKLTDTIGITFSPIRESLITVSKFNQFLSDKRH
                     EK"
     gene            18939..19967
                     /locus_tag="PLI16_00080"
     CDS             18939..19967
                     /locus_tag="PLI16_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH82984.1"
                     /translation="MKNKTIKYFTAVALCALVFACNNPDKNKAESNNTGQSKGATVNV
                     PSFNEDSAFLFVSRQTDFGPRVPNTAAHANCALWLEETLKKYTGNVVVQQAKVRAYNG
                     ALLNMKNIIASFNPDTPGRVILCSHWDSRPYADWDEDESKRRTPIDGANDGASGVGVL
                     LEVARIISTHKLNTGVDIILFDTEDYGEPQDDNNEYREDNWGLGSQYWSKNPHVPNYK
                     ANYGILLDMVGVENANFTKEGFSMQYAPDVVNKVWEAASRLGYGNYFSAEQTAPITDD
                     HYYINKISGIPTIDIIHHDSQTRSGFYKFWHTTGDNIGNVNKNALKAVGQTLLTVLYE
                     ESAALSQQ"
     gene            complement(19954..20679)
                     /locus_tag="PLI16_00085"
     CDS             complement(19954..20679)
                     /locus_tag="PLI16_00085"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOH82985.1"
                     /translation="MTKLSVNINKIATLRNARGGNLPDLLQVALDCERFGAEGITVHP
                     RPDQRHIRFADVYELKKSITTEFNIEGFPSEAFIRLVTEVHPAQVTLVPDAPDAITSN
                     AGWDTIKNAAFLKDIVSGFKQQGIRTSIFIDPDETMIAHAVVTGTDRVELYTEDYAKN
                     YFTDREKAILPYKKAAEKAMEVGLGLNAGHDLNLHNLAYFSKTIPGLMEVSIGHALIC
                     DALYYGLENTIQLYLRCLQPTAD"
     gene            20748..21410
                     /locus_tag="PLI16_00090"
     CDS             20748..21410
                     /locus_tag="PLI16_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH82986.1"
                     /translation="MLANELYTDAVIPVLTSDTANYALKLMEDQKICHLPIVNNVEFL
                     GLISENDIYALNKPEASIGSHPLSATNAFVYQYQHIFEIISLAGELNLSIVPVVDNKM
                     RYLGSITLPNLLEKFSQTASIQNPGGIIIVERNQNNYSLEEIARIVEANDAKVLNCFI
                     STFPDSMKLEVTIKLNKMNIFPILQTFERYGYVVLASFTEAEYKNDLQDRYDSLMNYL
                     NI"
     gene            21421..22299
                     /locus_tag="PLI16_00095"
     CDS             21421..22299
                     /locus_tag="PLI16_00095"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOH82987.1"
                     /translation="MRIALFATNYSAEQEPFYIEFLGKLIYNKFKVSIYEPLHKALLA
                     NHLINADIGTFCCYDDLKGKTDFLFSVGGDGTMLKTLFLVRDSGIPILGFNTGRLGFL
                     SAISRDEISEAIESLKENKYYIEKRSLIRLEKPENLFGADIFGLNEITIHKKDSSSMI
                     TIQAYVNDVFLNSYWADGLIVSTPTGSTAYSMSCGGPIIAPDAKNFILTPVATHNLTV
                     RPIVIPDSAVIKLKVEGRTNNFLVTLDSNNAVISNSIELVIKKEDFGINLVQLNSKSF
                     FSTIRDKLMWGWDKRN"
     gene            22388..23341
                     /locus_tag="PLI16_00100"
     CDS             22388..23341
                     /locus_tag="PLI16_00100"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOH82988.1"
                     /translation="MKKTITILLLIILFVGTAMAQQRRVFGKYDWRKDRHKVYLALGA
                     SEFYGDFGGLNKIGSETFSLRDFDIQAVRPAVQIGYIYRFSRFFASRSSFDYIWLSGN
                     DKWTKETFRRNRNLNFRTATYGLSSSIVFLYDFRQKTRFSGLHRTRSRARSGFREMVY
                     TPYIQAGVGGFYFNSKGKYNGEWYKLKPLCTEGQTLVPTRKKYSSVQLAIPVGVGFRI
                     KIAKEWEIGLEYSQWITFTDYIDDCSTTYFDENALLQNKGQLAVDMANPAIDDQVGMP
                     LYPSTRPGQQRGDPRDNDHMIGLFFTVCYSITEGYTPKLRF"
     gene            23348..24214
                     /locus_tag="PLI16_00105"
     CDS             23348..24214
                     /locus_tag="PLI16_00105"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOH82989.1"
                     /translation="MKKSLLLVFLSLFLVCGLRGQDFYRSEVGLFVGGSYYLGDLNPS
                     KHFLQTKFAGGAIYRYNITPRWALKINALLGGVEASDAVSKANIDRNLSFKSYIFDFS
                     TQIEFNFLQYIPGHKKNFISPYIFVGASVFNFNPKAQYEGNWYALHSLGTEGQGTTIE
                     GVKKPYSLTTFAMPFGLGVKYSPYKWISLGLEYGIRKTFTDYIDDVSTVYADPVILAA
                     ENGAVAAALADPSLSNTVEGHDYTGLKRGNSKTKDWYVFTGLTVTVHIRTKKARCDAY
                     KQHPKIKIKYKE"
     gene            24233..24973
                     /locus_tag="PLI16_00110"
     CDS             24233..24973
                     /locus_tag="PLI16_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOH82990.1"
                     /translation="MCMKEKINPKKLPEHVAVIMDGNGRWAQKRGAMRIFGHENAVTA
                     VRETVEAAAELGIKYLTLYTFSTENWNRPKTEVDALMNLLVKTINNETKTLIENNIRL
                     IAIGDLTSLQKDCYNELMNTINITSANSRMTLILALSYSARWEITEAVKQIAQQVKNN
                     QLDVNDIDQHTISSTLSTKDIPDPSLLIRTSGEYRISNFLLWQLAYTELYFSPVLWPD
                     FRKNDLYNAIIDFQKRERRFGMTSEQIK"
     gene            24978..27596
                     /locus_tag="PLI16_00115"
     CDS             24978..27596
                     /locus_tag="PLI16_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOH82991.1"
                     /translation="MLKNTFKLLILLFFSIPLQAQISLDDKGNIDYATPKEYEIQKIA
                     FSGVQYTDTNICKLLTGLYEGQKIMIPGEKTSTAITNLWNQGLFDKVRLYATRVEENK
                     IWLEIELKEHPRLAAQPLFSSNVKKSEVDDLREKINLTKGDIITPNVIQRSQNTIKTY
                     YHDKGYYNAEIKIKEIIDTASGKYSTKLNIDITRNKKVKVRKINITGNDHVSDFRLKR
                     LLKKTKEKTVFNPFMGCDTLLFQSVKKLFVRDSISIEDNFLNYINERVNLRIFKSAKF
                     LKDEYNKEKLAIIDKYNELGYRDSKIIADTIIRSGENTVDINIDIKEGRQYFFRKITW
                     IGNTIYSERELNAILKVKKGDIYNQKQLETNLSYNPEGYDVSSLYMDNGYLFFSANPI
                     ETAVENDSIDMEIRIIEGKQAYINKVSIRGNTKTKDHVVIREIRSKPGQLFNRSDIIR
                     TTRELAQLKYFNAEKIIPDIIPNPADGTVDIEYSVEETSSDQLELSGGWGMGRLIGTL
                     GVSFNNFSTKNFFKKSAWSPLPSGDGQKLSIRAQTNGKYYQAYNASFTEPWLGGHKPL
                     ALSVSVYYSSQTNGLSKSDSNRQAIGIAGASVGLGSRLKWPDDYFTLYHEISYRNYKL
                     KNYYSTFDFTDGTSNNLNYTVTLSRNSIDAPIYPRSGSEVSLMAQLTPPFSLLNGKDY
                     SKMAETDKYQWIEYHKWKLSASVYTKLAGNLVLSVRTKFGFLGSYNKKELGVPPFERF
                     YLGGDGLSGFSLDGRELIALRGYTNNSLTPRNSNGYIGGSIFDKFTVELRYPISLNPM
                     ATVYVLGFAEGGNAWDKWKDFNPFDIYRSAGIGVRVFLPMFGILGLDWGYGFDNIPGV
                     GKGGSQFHFSINQSID"
     gene            27622..28185
                     /locus_tag="PLI16_00120"
     CDS             27622..28185
                     /locus_tag="PLI16_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004356236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOH82992.1"
                     /translation="MKKLFVLLFVFAAFTLNSNAQKFAYVDSEYILENMSEYRDAQEL
                     LDKLSLDWQKEIEAKFAAIDKMYKDYQAEAVLLPEDIKKKRQDEIIAKEKEAKDLQKK
                     RFGKDGDLYKKRQELIKPIQDKVYDAVESIANASGYAVVFDKSGSTTMLYANSKNDRS
                     DDVLEKLGYKPGAIKTNTGGKNQDDEE"
     gene            28234..28749
                     /locus_tag="PLI16_00125"
     CDS             28234..28749
                     /locus_tag="PLI16_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOH82993.1"
                     /translation="MKNLTKIVLIIFLAGLTTVSFAQKKQKFGHIDSNELLKLMPGRD
                     SAEIKIRDYATTLENQLKGMQSELETKYQTYLTNEANYTDLIKQTQQKELQDIQARIE
                     AFQASAQDDLEKKQNELLKPIIDKAKAAIEKVGKDNQYTYIFDAGIGVLLYSDPTEDI
                     LPMVKAELNLK"
     gene            28849..29973
                     /locus_tag="PLI16_00130"
     CDS             28849..29973
                     /locus_tag="PLI16_00130"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOH82994.1"
                     /translation="MKHLSTFLLTTLVIVVFSSTIRVQAQGKNQVHINGVIKNNPFTN
                     VSLYQMGRENKFIADTVIAADNTFALACNVDKTDMYKVQFANNNFVILVLQPGEKVDI
                     SIDANDFLKKIQIKGSETSADIYRNQNLITSHKARIDSINNLSRTAAANPKYDSISKV
                     YRAETEKIKKSMDENLKAYIQKKPESLAILFLPEVLPVDNHIDAYLLADAALMAKHPD
                     NVFVQNLHMQITNSQKTAIGTLAPDFSLPDTTGTQIALSSFRGKYVLIDFWAAWCGPC
                     RREMPNMLKLYNDFKDKNFTILGVSLDKTRANWVGAIRSDKLPWTQVSDLKFWQSEVA
                     GLYDVKAIPYTVLLDKEGKIVAKNLRGEDLYQKIADLLNQ"
     gene            29976..30641
                     /locus_tag="PLI16_00135"
     CDS             29976..30641
                     /locus_tag="PLI16_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH82995.1"
                     /translation="MTASPDNIIQKTIDFVKSSSSGEASGHDWWHIFRVHNMAVYLAR
                     EEKADLFVVELAALLHDIDDWKFNAQGSGKAFAWLTQCGLQENIIQQILEVIDTVSYK
                     GAGEPNHAISIEAQVVQDADRLDAIGAIGIARTFAFGGSHGREIYNPHCPPVLHANFE
                     AYKKNQSHTINHFYEKLLLLKDRLNTNSAKALAAERHAFLEKFLAAFFAEWNFNAGTE
                     TQK"
     gene            complement(30632..31885)
                     /locus_tag="PLI16_00140"
     CDS             complement(30632..31885)
                     /locus_tag="PLI16_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH82996.1"
                     /translation="MKRLILLTLTFIPLLAYAQEKTDKFGIHFSGYVKNDFFYDSRKS
                     VGIREGHFHLFPENIRYDADSVDINAKTTFNILSIQSRLRGDITGPDAFGAKTSGVIE
                     GEFYGTTDASINCFRVRHAFAKLSWATTDLTVGQTWHGMFFEECFPEIMDVNTGCPFQ
                     PFSRNPQIKVTQHWKDFKFILSVMSQRDFAEWAGPEILRNAALPELNLRISYQHFNED
                     KTREIFVGGSADYKVVVPRIVTDSNYATNSSLNAYAFSLVFKYRCPKVTFKLSGVYGQ
                     DLYNLTMLGGYAYQHTTDSLTIARGDFSYTPLNTVAGWLDFTTNGSKFKFGILGGFTK
                     NLGSYNNILDWKDAGSYFARGWNISYVYRVSPRFVFQSGKIKMGLEGDYSVAGYGSKI
                     NSLGEVSEVKPVGGIRLTYSLFYYF"
     gene            32157..>32500
                     /locus_tag="PLI16_00145"
     CDS             32157..>32500
                     /locus_tag="PLI16_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH82997.1"
                     /translation="MNFKKLKILMIGLILITTPFLSMGQSKQEKTDTIITKLKLIEGQ
                     KMNYEFQIEPLKYQATGKDSIEIVELEKKLTDDEFVTLIRSAINETFSEDEINDIYYF
                     IQTSAFEKFFKSG"
BASE COUNT         9297 a         7276 c         7003 g         8924 t
ORIGIN      
        1 aataagccac cacacctcct acaatcagga tgagtgggat aaatatccac aacaaaactt
       61 ttacaatttt ttttccttgt gccataggtt taattttatt tatcaaacaa atatactata
      121 aaagctcttt tacaaagctt caaaatattt ttaattatga acggatttat tggttaatta
      181 ttttttcgat gctttgccac aagagcgctg ccgacttttt ccacgaaaac gctgtatgac
      241 gttgccggcc tttcgcaatt agactttggc gcaggttatc atcgccggct atttgtgtca
      301 tggcgctaca aattgactcg ggttgttgcg gattagtcaa aagggcggca ccaccagcta
      361 cctcaggcat ggaggtaaca ttggaggtaa tcacaggcac atcgcaacga aaagcttcca
      421 gaatgggaat accgaaacct tcaaaataag aaacgtaggt gagcgcaagg gccgcgccga
      481 ggacatcatt cagctcaccc gagtcgagac gtcccgtaaa aaggatatca tttttgtatt
      541 ccagcccacc aagtacagtt tccatttcag gcgtccacca catccttgcg cctgccatca
      601 acagcttaat gttaccggaa tgttgcttgc gaaacatatc ataggccaaa aaaaggtttt
      661 tgatattttt gcggggcagc agggcgccga tatacacaaa ataatcacac cccttggtat
      721 atcgctgttt gatgatgttt ttttgttcag cagcaatggg agcaaaattt tcgttaactc
      781 cattataaac cacatcaatg tagtcagcgg caatgccata agtagcagaa atatcctgtt
      841 ttgaaaattc cgaaacggtg gcaatgcgtg tagctttacg tgcaaaacgc ggaaaaaaat
      901 accggtagta tctgctgatg atccacggca ggtctttggg ataatgttca aaattcaggt
      961 cgtgcatcac cagcagttgt tgcacacgac accttagcga accgtagccg tcgggcgaaa
     1021 gaaacacatc ggcctttatt ctttttagta cccgcggcac cgaaaactca aaccacaaat
     1081 aaaacaacaa gggatggcgc gcctgaggcc ccaccacgac aggaaccaca ttatcggaaa
     1141 aaataaattt tttgtgatac ggccggtcaa aaataaaata aaaagtatgt tcgggatggg
     1201 ctgtcacaat gcgcctgagc gtttcaaagg taaaccaccc gatgccttca agcctgttgt
     1261 caagcagcaa acgcgtatta acggcaatgt tcacaacagc ccggattaat tgtcaatatt
     1321 gtattctttt atctttcggt ataaagtacg ttcagatatg cccagttctt tggcggcgtt
     1381 tttgcgtttg ccgtgatatt tgtcgagggc ttttttgatc atctctatct cccggtcctg
     1441 caacgagagt gattcctcat acacatcggg atcctgtatc ggctcggcaa tataagtttc
     1501 cggcttgtta atcttaacag ccggctcctg ggttgttacc gtttcataat cctgatgata
     1561 attctgcaaa aactgattct ttatatcctg tgcattcacc ggaacattgc cttctttaag
     1621 gatatccgcc acgagatgtt tcaggtcggt aatgtctttg cgcatttcaa acaataccct
     1681 gtacagcagg tcgcgctcgg atatttcggc tttgtcggcc ggctgacgca acaatacagg
     1741 gagtttgctt tcgctgctat ggggcaggta acccgacaag gtagccgcgt ttatattgcg
     1801 ctgtttttcg atgatggaaa tctgttcggc aatatttttc agttgtctga tattgcccgg
     1861 ccagcggtag gccagcatct gtgcacggga ttcctcatcg agcactattg gaggcatttt
     1921 atatttttca gcaaaatcgg tagtaaactt gcggaacaac agcagtatgt cgtctttgcg
     1981 ttcgcgcagc ggcgggatgg tgataggcac tgagttcaga cggtaataaa ggtcttccct
     2041 gaacctgttc tggtgaatag cttctgtaac attgacgttg gttgcagcta tcacgcgcac
     2101 gtcggttttg agaactttgg aggaacccac cttgataaac tcgcccgatt cgagcaccct
     2161 gagcaggcgt gcctgggtgg gcaggggcat atcggcaatc tcgtcgagaa aaatagtgcc
     2221 gccgtttacc acttcaaaat atcctttacg ggcttcgtag gctcctgtaa acgatccttt
     2281 ttcgtgaccg aagagttcgg agtctatggt tccttcgggt atagcgccac agttaacggc
     2341 aatgtaaggc ccgtgtttcc tggcactgag ttggtgaata atctgtggaa aaaactcttt
     2401 ccccgcaccg ctttcgcctg tgatcagcac actgagatcg gtaggggcca cttgccgggc
     2461 tatgtcgata gccaggttga gttgtggcga atttccaata attccgaaac gttgttttat
     2521 tgcttgaata tccattactc taaatatatt ggatgcaaag ttagttgttt ttttagtagt
     2581 tgccaagttt ttatgccaaa atggcagtta gcgaagagat ggatgcgtgg aagtaaggta
     2641 gcatttgttg gatgacgcta ataaaaaaat gtcagattgt tgttggtagc tgataccctc
     2701 taaaccgccg ctcctgggtt catgtgaaaa aaaaaaccgg cttcagacga aaccggtctt
     2761 ttatcagtaa caacccaatc ttacatcatg ccgcccatgc cgcccatacc ggggttcatc
     2821 atgggagcag ccggggtttc ttccttaatg tcgctgatca cacattcggt ggtcaggaac
     2881 atacctgcga tagatgcagc gttttcgagt gccacgcggg ttactttggt ggggtcgata
     2941 acaccagatt tgtaaaggtt ttcataagtc tctgtgagtg cgttgaaacc gaagtcgcct
     3001 ttgccttctt tcactttctg aataattaca gagccttcca gacctgcatt ggccactatc
     3061 tggcgcagtg gttcttccaa agcccttttg ataatagcga tgccgatttc ttcgtcttcg
     3121 ttggcgcctt tgagtttcga aagggcatca atggtgcgga tataagctat accgccgccg
     3181 ggcactatgc cttcttcaac agcggcacgg gtagcgttga gcgcatcgtc gtaacggtct
     3241 ttcttttctt tcatttccac ttcgcttgca gcacccacct ggataacagc aactccgccc
     3301 gataatttgg ccaggcgttc ctgcaatttt tcacggtcgt aatcagaggt ggtgttttct
     3361 atctgagttt tgatctgtgc tacgcgggct ttaatctgtt cgtggcttcc tttgccgctc
     3421 acgatagttg tattgtcttt gtcaaccaca actttttcgg cttgtccgag gtaagacagt
     3481 tctgcgtctt cgagtttgta gcctgtttct tccgaaatca gcgtaccgcc ggtcagtatg
     3541 gcaatgtctt cgagcatagc ttttcttctg tcgccgaatc caggggcttt aacggcagca
     3601 attttcagcg agccgcgcaa tttgttaacc accagggtgg ccaaagcttc ggcttccaca
     3661 tcttcggaga tgatgagcag cggacgtccg gtctgcactt ctttttccag aataggcaga
     3721 aaatctttca tcaccgaaat tttcttatcg tagatcagga tgaaaggatt ttcaaaaacg
     3781 gtttccattt tatccgtatc ggtaacaaaa taaggagaaa tgtaccccct gtcgaactgc
     3841 ataccttcca ctacttttac aacagtttcg gtacctttgg cttcttcgat ggtgattaca
     3901 ccttcttttt tcaccttggc catagcttcg gctatcagtt ttccgatagc gttttcgttg
     3961 ttagctgaga tggtagctac ctgttctatt ttgtcaaagc tgtcgcctac cgacactgat
     4021 tgttttttca ggttttcgat gacattttgc acggctttgt cgataccgcg tttcagatcc
     4081 atgggattgg ctccggcggt aacatttttc agtccggtgg taatgatggc ctgagccagc
     4141 acggtggcag tggtggtgcc gtcgccggcg aggtcggctg ttttagaagc cacttctttc
     4201 accaactgtg cgcccatatt ttccacaccg tctttcagtt caatttcttt ggctacagta
     4261 acaccatctt tggtaatctg gggtgcgccg tattttttgt caatgataac attgcgccct
     4321 ttggggccaa gagtgatctt taccgcattt gataattcgt ccactccctt ttttaaagct
     4381 tcgcgggctt tgatatcgaa aattatttct tttgccattg tattaatgta ttaaattgtt
     4441 ttattaaaaa aattgaatta cagaaccatg taaatatcgt cttctctcat gatgaggaat
     4501 gtttttccat caatcaccac ttcggtgcct gagtattttc catagagtac cacatcgcct
     4561 tctttaacgg tcatgggttc gtcttttttg cctttaccta cagctactat ggtgcctttc
     4621 tgaggttttt cctttgcggt gtcgggaata ataattccgc cggctgtttt ttcttcagcg
     4681 gctgccggtt caacaagtac ccggtctttc atgggggttc caaatttttt tgacatagtg
     4741 ttatgatttt tgtgtgttaa aatgttttaa aattttaagt tgagtggtta gcgattgtca
     4801 cattttgtgc caaggcaaaa aacaaactgt ttatagacat ttttacagtt tctccggcaa
     4861 aaaataaaaa aaatgtcaga atattgatga cattctgaca ttttaaaaaa gttaaaaaaa
     4921 acttactggt ttttgggagc cgcaggtgcg ttgggatttt cgggggccgg ggtataatcc
     4981 actgcctgat tattttccac ctgttccctt aattcggatt ctacggctgt gtctttgttg
     5041 gcacggggta tcacaaaaac cgaggacaag gtaagcacta acagcgctat cgccagtgtc
     5101 caggtggctt tttcaagaaa atcagctgtt tttcttacac ccataaactg gttggatgat
     5161 tgaaagttag aagccagacc accacctttg gagttttgta ctaacacgat gagcaccagc
     5221 aaaacacaaa caataaaaat caatacggat attacaacat aaattcccat gtctatatga
     5281 ttttaaatca gttaattatt aatttttgat tccagatttt caatttgggc tgcaaagtaa
     5341 gcgctttttt ccggattaag caaatataat ttttgataaa ttttaagggc tttttgcaat
     5401 aaaccttgtg agatatagat ttttgcgagg gtctcgctca caatttcttc tttgtcaaca
     5461 ctgctgtttt cggccatatt aaccggattg aagaagtcgc gtttcggcgc cgaaatccgg
     5521 ggctcttcct tgataaactt ttcgataagc tcggtatttt tttcgggaag caccgtttcc
     5581 tttttctccg tgaccgatgt tggtaaagcc tctttttcac cagagtcttc cgacaaaatt
     5641 cttttcgcca tgatgttcag caggaaacct gccggctggt tgtgcgaggc gggagtgatg
     5701 atttttgccg gttcttcagt gggcatttct tcctcgcttg tctcattttc aacaactgtt
     5761 tccatcgcgc cctgagcaac ttcctgagac agacgttgtt cctcttcatc aattccaggc
     5821 ccggttattt caactgtttg tgtggatacc gatagttctt ctgccttttc agcttcaacc
     5881 tccgcgtttt gctgcggtac caactcctga gctgattcca ttttgtcctg aacttgttcc
     5941 tgcgaagttt tttcctccgt tacaggcagt tcttgagtct gagataccat accggaaggc
     6001 aaatccggct gctgctgaac ctcagggagc cgttcctgca tggtggtggc tgcggtttcg
     6061 gtggctactt gtctggcaat gctaaaggga gtttctggta cctccacagg ggtttccagc
     6121 ccggttccag gtaatgtggt gctgacgtgc gtctggttgt aaatgagcca atacaataca
     6181 gatctgtcgt tgacatgcgc tgcggtcagt tttagctggt cgtgaaaagc tatatcatcc
     6241 tgactgctga gataagccgc atataccaaa tgtgccgact gaaaataggg aaacttttcc
     6301 agcaggaaag tcaactctcc ggctttgctg ttacggatgt tttcgggcct gctgagatac
     6361 cctgtgaagt cgtttttatt catgatggat taccagttta cggcggtttt gttaaaaaca
     6421 tcatccacca ggtaactgat gatttcttcg agtatctggt tttctaccga tgccagcgcc
     6481 tgcgtgctgg gatagtccac atagcgcgaa aacgaagact cgtaattttg tttttcgtcg
     6541 tatttgttaa caaaacgtat gctgatagtt acagtaagcc ggttcatggc ggcagtctgg
     6601 tttccctgga tagccaccgg tgaagtggcg taattggtaa tctcgcccga aatgtcaaaa
     6661 tcaccgttct gattgacagg tgtcaggctg gtttgggtag aaaagcggtt gcgcaactcg
     6721 tcggtaagct gctggctcag ggtaggcttc accaataagg cggtgttttt caggtaatga
     6781 atggaaatgg tttttacttc gggcggtatg gatgcgccgg taaaagaata tttcatggta
     6841 caggaggtca tcagcagggc cactgccata caaagcgcat aaaataatcc cttggggttc
     6901 acctgatttt ttttacaaag gtagtaaaaa tgcataacat tgcacgttgc tgacaacaaa
     6961 cctttccgta aaaaacccac ccttcagcat caatttatta tgtaattcag cagggtatta
     7021 ctgtcggaaa ccagtatgct ccttgaattt ttcccaaaca atgaagtgat ctcaatctcg
     7081 ggactaccca gaggataatt caataattct tccatccctt tgttgtttag cagcaccagg
     7141 tgtgagcagg ccgaatcggg aaataccagc tgaaacttgc tcctgctcag gcctgcatat
     7201 tgaggcttgc ccgcaggatt accttttagt cgtgcttcaa agattttctt ttttacatga
     7261 ttgtaaacaa tcactttccc catatcgaaa aagataaaat cattccattt gtccatatca
     7321 aaatctccca ccaggaaata atgattttcc gaaaaaggct gcagggtaac cacatcaatt
     7381 tttccgtttc cggaaataaa aatcattttt ccttttctgt cggtggtcag caaatactgt
     7441 tttttaccgt ccttatgtat gtagaacctg ctgtttttac ccttgatgaa cgcttgtttg
     7501 ttttttatca gcagggtgcc gttacggctg agaaaatata ctttgccatt tttgtccacg
     7561 gctatcatca tgtctttatc ctgaaacctc acatattcca caggctgttg cagtatggct
     7621 tcggtgcggt agttattcca tcccggtgta ggttttccgt tgagcagata attataaatc
     7681 ttttggttgg tacagggaat gataatacgg tagtcatcgt ttccacggta attgatgagg
     7741 gtaatggctg tgctggctgg atacggcaac ttcagagggc tgtagtcagg cttatttccc
     7801 aaaagatcga acgtatagat caggtttttt gtattgaaaa taagatagta cttattgtct
     7861 tttttcaggc tggtaacaaa gaccttgctc aggggcgctt ccctgatctg ctttttccat
     7921 atcagttgtc cgttgcgtcc cagcaaataa atgttattgg aggcgtcgaa aaaaactatt
     7981 tttttggtgc tgtcgcgggg gtcgataatc acggaaattt tgccgctgat gctggtatcg
     8041 gcatgatatt cccaaacggc agggttttct tccacataat cgctattatg ttttatatag
     8101 gcgttgagaa agaacttgct attatcggag gtaaactgca gggccagtgc ctgaaaattt
     8161 ttcatagccg tttcgtttcg caaaaaatcg gattgtagct ttttgttaaa caatggaagc
     8221 cataatccaa aactcttgcg gatattagtg tacaggcaga agttggcctc gtcggccaca
     8281 ttatcggaaa aatcgatgta attgagattt ttttgcagtg ttttgcccga gaggtagccc
     8341 gatacaaaca gatcgagcga cagcggggat tgcccgatga cgacataatc ccggatggtg
     8401 gtataataag cagtgtcaag agcatcgaac atggtgccga aaaaatcctt taaaaagttg
     8461 acaggcaaaa attttcttat ggcaaaagaa tcacggcggg ccaggaattg ttcttcggga
     8521 acaacggaca tgtcggccag agtttgtttg aaattgccca tgtgacgggc cttaaagacc
     8581 ccgtaataat taaagggatt ttcaatattg gggttttcgg taacaacaca ggcaaactga
     8641 ttaccaatct gattcaggaa tttttcagat atgttgattt cgtaagtagt gtcgtaatag
     8701 gccttcagtt cgttgaaagc atgccggtga aaatgggttt ttacaaaatc ggtataattc
     8761 tgtacatagg cattggcatt gctcactccc cagaaagtaa gtaccgccgt attggccggg
     8821 caaatgtccg tgagttcgat aggttgcgga gactgtccgc taaatacccg attgagcaac
     8881 tggtcctttt tgcccgacag ggcataaccg gcaaaaagca actcgtcatt ttttacagtc
     8941 aggtctaaac ctaccatatc gcccgtaagc gaaagaaact taattttgcg gtattgcatg
     9001 cctgtaaaat accccgaaac caggttatcc aaataagtgt tacggatgta aatgttggcc
     9061 tcaacatttt tgccagcggc agcattgagt ctcataaaat ccgcatcgtt ctccatactg
     9121 attttggtca gcagcgtgct cagcgacgat tcgaccactc ctgaggtttc ccctatgata
     9181 agtactccct gatataccgt ataggcacat attaattttc ctgctttata aatttcaaag
     9241 atctcatttt tcaaaaactg gtgcgacaag atggtgtttc cctgcgaaaa gtgcgctcgc
     9301 ataaattcat ccaccagctt ttcacggtgc ggcccgttga gggttatggc aaacagatag
     9361 cgatacaccg ccctgtcggg tacccaggcc acgtacaatg ggttttgtgt aagtatatcg
     9421 gctacatcgt catcagtatt ggccagggag tcgagtgcct gtaagccccg gttcaggtcg
     9481 gacacaaaac tcaggcattg caattctttc cagagattgt tcttttcgtg gagccgatcc
     9541 cagagcgaca gggtgttttt aaactccata aggcataccg tactttcggg gatagctttg
     9601 atggcaggta acacgggatc tttcagccgt ttcagatacg aataccccac aaccaataag
     9661 gcgacaattg ccagtaccac ccacccggta acgaagtacc ttctcgttct tatccgtctg
     9721 tcgctccttt ggtatttcat gacattttaa aaaagtaaaa gtacaacaaa tacttttctg
     9781 acaaaaactg attgctttaa aaaatgagtt ttaactgttc attcaggtta aagcttttcc
     9841 ggtgataagg cgtgattccg taaagattga tggcatcctt gtggtcgcgg gtggggtatc
     9901 ctttgttttg agcccagttg tattccggaa attcgtcgtg cagtgttatc ataaattcgt
     9961 cgcggtgagt ttttgccaat accgaggcgg ctgcaatgga caaatatttt ccgtcgcctt
    10021 ttacgataca ttcatgggga attttttcgt aaggtttgaa gcggtttccg tcaatcagca
    10081 acaattcggg tgttagcgaa agttgtcgaa tggcaaggtg cattgacatg atggatgcgt
    10141 tgagaatgtt tatctcgtca atccgcaggt tgtcgagctg agccacagcc caggccactg
    10201 cttctttttc aatttcaacg cgcagtatct cccggtgttt ttcgctcagt tgtttggagt
    10261 cgtttaatgc gttgttttta taatctttcg gtaaaataac ggcagccgca aataccggcc
    10321 ctgccagaca tccccgcccg gcttcgtcgc aaccggcttc tatggtgttt ttcctgaaat
    10381 acgatttcag catattctta ttcgctttac acttttctcg gggccaggaa cctgttatat
    10441 aacaacagcc cgatgatggt tatcacccct atcagactga atgtgaacca taaggccgac
    10501 ggattattta atttttcgat ataataaaca tacaaatttg ctcccagcac gcctccgatg
    10561 ccgcttccga tcacaccgta caaaaaggaa taacccagat acagggcttt tttatcggct
    10621 ggagctatca accccacgta ggaaataaat ttcggatgcg ctgtcatttc gcccagtgaa
    10681 aaaataatca agccagcaat aaatacccaa gcattagaag aagttccgag aattgccatc
    10741 cctattgttc ctaacaaaat tcccacaatc atggtcggca aggctttagt atgttttaca
    10801 atatttgata tcaatatctg cagcagaata atggttcccg cattgattac cgtaacatgt
    10861 tccgcctcaa atttccattg agaattagca acaaacaacg atagtactga attgacaaaa
    10921 tcgttcaccg gagtcatgtc catatgttcg ctcagatacc acaacacggt gtcgaacatc
    10981 tgaaaataaa gtatccagaa accggaataa ataacgatca tcagaataaa acggtaatct
    11041 tttatcacca gcagcgcatc tttgagaata gtaagcaggc ttttggaact ggccggtctg
    11101 gccggttcgc ggtacaccaa caggttgagc aacaacagcg agcccgtaat caccgaagcc
    11161 atgataaaaa tatagttgta agaatagctt tttaaataag gtatcaaaat aagcggaaaa
    11221 agaaaagccc caaggtttat cgaccagtaa aagatgccga agcccagcga agaatttttt
    11281 tcgtctgtcg aacgggcaat ggttccggaa atgataggtt taaaaaatcc ggctccaagc
    11341 accatcagca aaaggctggc aaacaccagc ggatagtcgg taaactggct ggtaagaaaa
    11401 tacccgcagc tcattactat gaaggcaaaa aacaaggttt tcctgtagcc gtaacggtcg
    11461 gctatggccc ccgaaataat aggcagcaaa tataataaag gcgtgatggt gcttttaata
    11521 accccgacgc tttctttgga aaaaccaagc ccgccgtctt gcgtactcat aacaagatac
    11581 accgataata ttgacatcac gccataatac gagccccgtt caaaaaactc catggtgatg
    11641 accgtccaga agtttttcgg gaaactcctg aaccctgatc ttttctttgc ctgctgctcc
    11701 attaatctga atttgattga gtatgtaatt accttagaaa caaagaatta taaaatagac
    11761 agtgatttat tttccaaaaa caaaaaattt tgatttttct gttccacata acccacaata
    11821 gtcttcaata tcttcattgg aatatgtaaa cccgcatttg ggacagacat agtattcggc
    11881 aggcaatgtt ttttcttttc ctgattttag ggcatcggca gccgcctggt aaagctccat
    11941 atgtttcatt tccgtatcat ttgcccagcc gaatgattta attgcatcat tattattatc
    12001 tgtttgtgcc tgttcaataa atccagggta catggattcc gtttcgtgtt tttccccttc
    12061 gtatgccgca agaagatttt ccagcgtatt attcaccgta aatgtttcag gctgcacatc
    12121 aactttatgc ccgagggttg ccagaacatt tctgtgattc gccgcatgta cgctttctgc
    12181 ttttgatgcc gcatcgaata gtgcggctat ccggggaagc ccttcttcgc cggctttttt
    12241 tgcaaaagct gcatattttt ccgaagccgt ggtttctccc tgcagggcag ttttcaggtt
    12301 gtcaaccgtt ttatttgtta cggcaacagt ttgttcgaca gatgcagatt ctaaaccaac
    12361 catgtgatta tacaccagtg tccctcccaa aagtccggta aaaccaacag tgcaggtggc
    12421 aatcagatat agaaaaaata caacccattt aagtccgctg ttgcttttct ttttccatac
    12481 aaggtaaatc cgcaaaagac tggcggcaag cataacaaac atagtaattt ttgcaaaaac
    12541 ttcatgacgt tcttttaatt cacccgcttc gcctgtaagt tcccgtgtga aaaattcacc
    12601 tgtaaaatag gctgcaaagg cgcctaatgt tcccagaatc atcagataaa acccaacctt
    12661 tgacagacag ggttcttttt ttgtaaagaa aagacttaat acatcggcca gaaaaccaat
    12721 cataatgagt gcaaccggaa agtgcacaat cattgggtgg aagtgggttg gatcaaacat
    12781 agtactttga atttaggttg ttttattgaa ttattttcat ttctgcaatc aggttttttg
    12841 ctccggcata tttatcaatg atgaacaata cataccggat atccacattg atggttcgct
    12901 gcagttcggg ttcaaaaaga atgttcccgc tcatggcttc ccagttgcca tcgaaggcca
    12961 gtccgataag gttgccctga gcgtcaagca ccgggctgcc cgagttgcct cctgtaatat
    13021 ccgtattgct gataaaattg acaatcatct cgccattgga accatacaca ccatagtctt
    13081 tgctgttgta caactctttt aggcgccgcg gcacagtgaa ctcccagtta tcggggtctt
    13141 ctttttgcat tacaccttcc agagtagtat aataattata atgtacggca tctgccggat
    13201 aataatcctg aacctttcca taggtgaggc gcatggtctg attggcatcg ggataaaatt
    13261 tctggtcggg cagcatctcg tggatacccg ccaccagcag gcgtttccct aacgacagtt
    13321 tatcattttg aaccgcccaa gcctctgaaa cgacattatt ttgctctgca aaagcattta
    13381 tcagcatcag cagggcatca gattccagta ccttagcgct gggtgcatcc aaaaagccgt
    13441 tcaccgtact caggtcgcca aaaatacttt tggaaaacaa ataggcactc aatttattaa
    13501 agtcgtattt atattttgca accagtttca ggaaataagc aggttgctgg ctttttggca
    13561 cctcttcgta gaacatgttc agcatagcgg ccgtcagctt tacatccgtc ggtttgttat
    13621 agtctttata aaacttttcg gctgcagtcc gcagttcgct ggttttagct ttcaacccgt
    13681 cagcatttac cggtttttcc ttaagcaggg gctgcatgct tttaaacctg cgcgaaaaaa
    13741 ccattgcttc gcaacccatg atggattcag aaaaataggt acgtgcctgg ttggtttttc
    13801 gcaattcggc ataggcctct ttaattgtga taaggacaga gccatacttt tctttgcgcc
    13861 cgttatcggc cataacccat gcttcaaact gagattccag gtgacgcttt ttatcggcca
    13921 ctttcagccg gttcaaagct tttacggtgc caataaaatt tttccagtaa ttggatatcg
    13981 aagcatattt tgaagaatat ttgatgcgta cttcggggct ggcatccata tcttcccgca
    14041 taatggccag tttcatttcg cgggctttga tccgggcagg ataggactga ttagcgtaca
    14101 tttctattcc gtaagaggta agataccgtt cggtggttcc cggatagccc atgatcatgg
    14161 taaagtcgcc gtttttcact ccttttatgg aaaccggtaa cgaatgtttg ggtttcatgg
    14221 gaatgttttt gtcggaataa ggcgccggag atccgtcggg ggcggtataa acgcgaaaca
    14281 aacagaaatc ggctttgtgg cggggccaca tccaattgtc agtatcggcg ccatacctgc
    14341 ccagtgatga aggcggcgca ccaaccaaac gcacatccgg atatgtttga tagacaatca
    14401 gataatattc atttccttca aaaaaactct taaccacagc atcataccca ttgtctttag
    14461 tggcctcttt ttcaatttta gcacataact cttccacttt tgccgtccgt tgtttttcgg
    14521 tgagattggc ctgaagccct gataatacct tttgggtaac atcttcgata cgaacaagaa
    14581 atttgaccga caggtcgcct atgggcagtt cttctttcat attcatcgcc caaaaaccat
    14641 ctgtcagata atctttttcc acggtgcttt ttgactgaat ggctccgtaa ccgcaatggt
    14701 gattggtaag tatcaatccc tgcgacgaga taatttctcc tgtgcagccc ctgccaaaaa
    14761 gtatgatggc atctttaatg ctggcatggt ttacgctgta tatctcctcg ggggtgagct
    14821 tcagccccat tttctgcata tccgtataat tcagtctgtc gatgaacatg ggcagccaca
    14881 tgccttcgtc ggcacggcct ttattttggg cacacaaaat ggcaagcagg cacatgaaga
    14941 acaacttttt cataattttg tcgtatttaa tagtttatag ttccggatac aaaataaagg
    15001 atttttttga caaaccctga cggtatttta aaactgagca acgcgctaca tgatagaaaa
    15061 aggttataaa tattttttgc tgatagtgtt aatactgttc agttcctgtc accgcgatct
    15121 tacagatcct tttattccga aagataaaaa attcgacaca cagtttaaat tcagcacccg
    15181 gaaaaaccgt accgtagaaa aacacagcag tttcgcccac agcaaaggag gctttaatga
    15241 ctcttttggt ttttccaggc ggggagggca cagaggcagt ggcggtaaag attctttttc
    15301 atttaaaccc tccaaaaaaa caggcggcgg ttttggtaaa gattcattcg catcacccag
    15361 gggcaaaaga accggtggcg gctttggaaa agattctttc tcgtcgaagc gcggaaaaaa
    15421 aacaggcggc ggcttttcca aagattctta ttccgcttcc ggaaaaagcc gcttcggaaa
    15481 agcggcgcca cggtttttgt cgaaacgggg cggcaaaaaa gacagctaca gcttaaaaca
    15541 caaaaatgtg tccggaatgt tccggttcag caaaacaaaa aacagcatcg taagcaaaca
    15601 aaaattcctc gtgttcagca atcataaacg catcaacgag agcggctcgt tcagggcaaa
    15661 gtccaaaaag gtctcgggac agttccgcta tagtggccac aaacgtatgg tatcgaaaaa
    15721 acgtatggtt ttcagcggag caaaacgcct gagggcttct gaaagtttta atgttcgggt
    15781 caagaggcat cccagagtct tcggttttaa tgcagaaaaa aaacgtgtgt cacccatagt
    15841 gtcgttgcaa cgccacaaag acaccgatgc cgggtcgttt ggacacggac ggaaaaacgc
    15901 aaacttttcc agacaatatg tcttcagcaa aaagcaaaaa cgggtcaaaa agcgcgatag
    15961 catctggtgg gatatgaacc agagttttca taagaaaaaa cgcaaaaaga aattgcttga
    16021 gcttgaattg tttgatccca agatgaaacg ggaaatgaaa ctgaaataaa aactaaaatt
    16081 ttacaatttt actagtgtac tgattgttac aggcttttac gcagaccaca taaattcctg
    16141 gttttaaggc tgaaatgtct gttccggctt cataaaaagc tgcattattg attctatcaa
    16201 tcaggcattt tcctgtcaaa tctttgatct gaacacggca gaaattattc tcaccaggca
    16261 aggtaataaa aagcctgctt gttgtgggat ttggaaaaat caaaatgtcc tcgggtgttg
    16321 ttatggtagt ttttgtgata agactgtgca agctgcaatc agtcaattcc atcgaataaa
    16381 accggtcttt tgcagggctg gtgccaacgt tcaatagatt tggtgtacca atatccggcc
    16441 atgccatact tacaccccaa aaagcaggtg cagtttccag gtaatatgaa ttatcattaa
    16501 gcacattggt gccattcggg acaatagttc cgttatcatt gttcccatat tgtatattac
    16561 cgctaccggt aatggtataa gcaccccatt gaaatgggaa ggtataatct gtgccggtga
    16621 tttcatttcc aacaaaattc tgattgttgg tcaaagttgc ggccataata attccgtacc
    16681 tttcagcacg gttgcggaaa aaagtattgt atggcccaga cggtccccag gtttcatcaa
    16741 tcatgatcgt ttgcacaata ttgccctcga acaggttggc aaacgaataa tgaccgtgaa
    16801 gtgaaatgtc tccgcaataa tcctgcgggt attccattga accgttcctg aaaggattca
    16861 gtgaataatt ataagcaaaa acattcccgt tagcaccttg tttggtcatc atggcatggc
    16921 gcaaatgatt gaaaatatta ttctgtataa gacacagagt ggcatggttg gtacatgtaa
    16981 tcccatagcc cttggtgcta ctgccatcat aaaggaacgc atcatgaaaa tagcatcctt
    17041 ccaagctgat atgagaactt aaccgtatca tgcaatgact tccctggctt ttgttgcttt
    17101 cgacacctct gattacgcag tttaccgcgt aaataaaacc aaaattatta cctgaattta
    17161 cggttgagct atcggtccgt gcaacattta tgcatgaaat acccgacgcc tttcggggat
    17221 tgattttctg tatttgcagg ttcagggaag tatcaaatgt gatgtgtaat ccgtcggcaa
    17281 gcactaaagt atcacctatt atggaatcca caagaatcat ttgcccgaca gaataggttg
    17341 cccaggtagc cggttcaaca tcccaggagc cattatcctg ccggatttcg caataattac
    17401 cgggttgaaa taaagcaggg tttgccacag ttattttatg tgattctttt tggaaacccg
    17461 aaataacatc tgtgaaaata ttggtggcgg tcccggaaaa ggaaaacccg gtgctgctgt
    17521 gtccaatctt tataaaggtg gaatccgggc ccgcccctgt caaccagaca gaatcaggga
    17581 tggaaaccac ggaagaaaac aaatacaaac cggcagggaa aaaaacgatc ccgggatgcc
    17641 caccaaaact ggccatggcg ttggtcagcg caacggtatt atcagtcgca ccatcagcta
    17701 ctccgccaaa cgaaaggatg ctgacttgcg gcgccgtaat gggaaaaaca aagctgctcc
    17761 ccgctgaaga ccaatcaaca cggcttgttt caggaaggac ttgagcagat gtccgggtag
    17821 taaaaagtac aagaaacaga aatatgaaag aaataatctt aaatgatttc ataaggaaac
    17881 aaaaatacta aacttatttc tccggattaa tattaatttt gtaaggagat aacataatat
    17941 gatactagta accggtggca caggatttct gggatcgcat acgcttatgc agctttgcga
    18001 acagggaaaa aaggtcaggg cgctgaaaag gtcggcgtcg gacaccgctt ttgtgcgaca
    18061 actgttttct ttttaccaca aagaacatct tttcgatagt atcgaatggg ttgaaggaga
    18121 catcctggat attcactccc tcctgccagc catgaaagac tgtgaacaac tttatcattg
    18181 cgccgccagc gtaggctttt caaacaaaga taccgccaca ctgacaaaaa acaatattca
    18241 gggaacacag aatgtggtta atgccgccct cgaaacggga ataaaaaaaa tgtgtcatgt
    18301 cagctccata gcggttttgg gcaaagaggc cctcattacc gatgatacat tttgggatgc
    18361 cggagaaaga cattcggcct atgctgaaag caaatataaa gccgaacagg aagtgtggcg
    18421 cggagtagca gagggaatgg atgtcgtggt agtgaggcca tcggtaatta tcggcccctg
    18481 gaaacagcag ggaggcatcg gggtgctgtt caggaagatt gaaaaaggat tgcgttttta
    18541 taccccgggg agcacagctt acgtggatgt gaacgatgtg gcccgcgcca tgataacact
    18601 aaccgacagc ctggtgaaaa acgacagttt tattgtatct gccgaaaacc tcacataccg
    18661 cgaactcatc ggtatgattg ccaacatagc agggaaaccc gccccgcgct ggtgtgctgg
    18721 ccgtttctta agcggcctgg cctggcggct ttttgtatta aaagattttt ttacccgcag
    18781 cgattctgga tttaatgaaa ttactgcaga aatctcgcag accacatccc tatactccaa
    18841 caaaaaactg acagatacca taggaattac tttttccccg ataagggaat cattgataac
    18901 agtttctaag ttcaaccaat ttttatcaga taaacgccat gaaaaataaa acaataaaat
    18961 atttcacagc cgttgcctta tgcgcgcttg tctttgcttg caataatccg gataaaaaca
    19021 aagcagaaag taacaatacc ggccagtcca aaggcgctac tgtcaacgta ccgtctttta
    19081 acgaagattc tgcttttctg tttgtgagta gacaaaccga tttcggaccc agggttccca
    19141 atacggcagc tcatgccaat tgcgccttgt ggcttgaaga aacattaaaa aaatatactg
    19201 ggaacgtggt ggtccagcag gccaaggtgc gtgcttataa cggcgccctg ctgaacatga
    19261 aaaatatcat tgcgtctttc aatcccgaca ctccgggaag agttatactt tgttcacact
    19321 gggattcgag gccttatgcc gactgggatg aagacgaaag caaacggcgc acacccatcg
    19381 acggggccaa cgacggtgcc agcggtgttg gcgtactcct cgaagtggca cgaataatca
    19441 gcacccacaa gcttaacaca ggggtggata taatactgtt tgacaccgaa gattacgggg
    19501 agccgcagga cgacaacaat gaatacaggg aagacaactg ggggctgggc tcacaatatt
    19561 ggtcgaaaaa cccccatgtg ccgaattata aagccaacta cgggatactc ctcgacatgg
    19621 tgggtgtgga aaatgcaaat ttcaccaaag aaggtttttc catgcaatac gcccccgatg
    19681 tagtaaacaa ggtgtgggaa gccgcctcgc gcctcggtta tggcaactat ttttcagccg
    19741 aacaaaccgc tccgattacc gacgatcact attatatcaa taaaatttcc ggcattccca
    19801 ccatcgacat catacatcac gacagccaaa cacgcagcgg gttttacaaa ttctggcaca
    19861 ccaccggcga caatatcgga aatgtgaata aaaacgcgct gaaagcggtg ggacaaacct
    19921 tgctgacagt actgtacgaa gaatcagcag cgcttagtca gcagtaggtt gcagacaacg
    19981 cagatacaat tgtatagtat tttctaatcc ataatacagc gcatcacaaa tcagcgcatg
    20041 acctatcgat acttccatca ggcccggaat ggtctttgaa aaatatgcca ggttgtgcag
    20101 gttgagatcg tggccggcat ttaatccaag tcccacttcc atagcttttt ccgcagcttt
    20161 tttatatggt aaaatcgctt tttctctgtc agtaaaataa ttttttgcat aatcttcggt
    20221 gtacagctca acgcggtcag tgcctgttac cacagcgtgt gctatcatgg tttcatcggg
    20281 atcgataaag atagaagtac gtatcccctg ttgtttaaat ccggaaacga tgtcttttaa
    20341 aaaagcagca ttttttatgg tatcccaacc cgcatttgat gtaatggcat cgggcgcatc
    20401 agggacgagg gtaacctggg ccggatgcac ctctgtcacc aggcggataa atgcttcaga
    20461 aggaaagccc tcaatattaa attcggtggt aatacttttt tttagctcgt acacatcggc
    20521 aaaccttatg tgccgctggt cgggacgggg gtgtacggta atcccttcgg ccccgaaacg
    20581 ttcacaatcc aaagccactt gcagcaaatc gggcaaattt ccaccccggg cattgcgaag
    20641 cgtggcaatt ttgtttatgt ttacactcag tttggtcatc acagatattt ttatgccaaa
    20701 ggtaaaaatt agtatgtttg taaaaaatta ttttcacgga aagttatatg cttgccaatg
    20761 aactttatac cgatgccgta attcccgtgc tcacttccga tacggccaac tatgcgctga
    20821 aactcatgga agaccagaaa atctgccatc tgcccatagt gaataatgtg gaatttctgg
    20881 gtttgatttc cgaaaacgat atctacgcat taaacaaacc cgaagcatcc atcggcagcc
    20941 atcccctttc ggcaaccaac gcgttcgtgt accagtacca gcatatcttt gagataattt
    21001 ctctggccgg cgaactaaac ctgtctatcg tgccggtggt tgacaacaaa atgcgctacc
    21061 ttggatccat caccctaccc aatctgctgg aaaaattttc gcagaccgcc tcgattcaga
    21121 acccgggcgg catcatcatt gtggagcgca accaaaacaa ttattcgctc gaagaaattg
    21181 caagaatcgt ggaagccaac gacgcaaaag tactgaattg cttcatcagc acttttcccg
    21241 actccatgaa actggaggtt accataaaac ttaataaaat gaatatcttt cccatcttgc
    21301 aaactttcga aagatacgga tatgtggtat tggccagttt taccgaagcc gaatacaaaa
    21361 atgacctgca ggaccgctat gattcactga tgaattatct gaatatttaa ctattgatat
    21421 atgaggatag cactttttgc caccaattat tcagcagaac aggagccatt ttatatcgaa
    21481 tttctcggaa aactaatata caacaaattt aaggtttcca tatacgaacc tttacataaa
    21541 gccctgctgg ccaaccacct gataaacgcg gatataggca ctttttgctg ttacgacgat
    21601 ttaaagggaa agactgattt tctatttagt gtgggcggtg acggcaccat gctgaaaacc
    21661 ttgtttctgg tgcgggattc ggggattccc attctgggtt tcaacaccgg ccggcttggt
    21721 tttttatcgg caatctccag ggatgaaatc agtgaagcga tcgaaagcct gaaagaaaac
    21781 aaatattata ttgaaaaacg ttcgctgata cggctggaaa aaccggaaaa cctttttgga
    21841 gcagacattt tcggacttaa cgaaattact atccacaaaa aagactcttc gtccatgatt
    21901 accatccagg cctatgttaa cgatgttttt ctgaattcct actgggctga tggtctgata
    21961 gtttctaccc ccaccggctc caccgcttat tccatgagct gtggaggccc tatcatcgct
    22021 ccggacgcca aaaatttcat tctgacaccg gtggccacgc acaacctgac ggtcaggcct
    22081 atcgttattc ccgacagcgc ggtgataaaa ctaaaagtag agggccgtac taacaacttt
    22141 cttgtaactc ttgactcaaa caacgcggta atcagcaatt ccatcgagct ggtgataaaa
    22201 aaagaagact tcggaataaa cctggttcag ctcaactcaa aatcattttt ttcaactatc
    22261 agagataaac taatgtgggg ctgggacaaa agaaattaat cgtttcttta aaaacttgat
    22321 aattttggtg ttgtttgaaa tttttatact tttgtaaatt cagtgtacaa taacttattg
    22381 taaaataatg aaaaaaacaa ttaccatcct gttgttaatc atcttgtttg tgggtactgc
    22441 catggcccag caaagaagag tattcggcaa atatgactgg cgaaaagacc gccacaaggt
    22501 ttatcttgct ctgggagcct ccgaatttta cggtgacttt ggcggattaa acaagattgg
    22561 ctcggaaaca ttcagcctga gggatttcga cattcaggct gtccgtccgg cagtgcagat
    22621 aggttatatt taccgttttt ccagattttt tgcgtcccgc tcctcttttg actatatctg
    22681 gctgagcgga aacgacaaat ggacaaaaga gaccttccgc cgcaaccgca acctgaattt
    22741 ccgcaccgca acctacggac tgtcatcgtc tattgtattt ctttatgact tccggcaaaa
    22801 aaccagattt tcgggtttgc accgcaccag atcccgggcc cgtagcggat tcagggaaat
    22861 ggtatatacc ccttatatac aagcaggtgt tggtggcttt tattttaatt caaaaggaaa
    22921 atataatggc gaatggtata agctaaaacc cttgtgcacc gaaggccaga ccctggtgcc
    22981 caccagaaaa aaatattctt ctgtgcagtt agctatcccc gtgggtgttg gattcagaat
    23041 taaaatagcc aaagaatggg aaataggtct tgaatacagc cagtggatta cttttaccga
    23101 ctatattgac gattgcagca caacatattt tgatgaaaat gcgttattgc agaataaagg
    23161 acaattagca gttgatatgg ccaatccggc aattgatgat caggtaggca tgcccctgta
    23221 ccccagcacc aggccgggac aacaaagggg cgaccccagg gataacgacc acatgattgg
    23281 attgttcttt actgtatgct actcaataac agagggatac accccaaaac taagatttta
    23341 atgttttatg aaaaaaagtc tcctgcttgt ttttttatcg cttttccttg tatgcggact
    23401 cagaggccag gatttctacc gttccgaagt cggactgttt gtaggcggtt cgtattatct
    23461 cggtgacctc aacccctcga aacatttttt gcagaccaaa tttgccggcg gcgccatcta
    23521 tcgctacaac atcacacccc gatgggcctt gaaaatcaat gccctgctcg gtggcgtgga
    23581 agccagcgac gccgtttcca aagcaaatat tgaccgcaac ctcagcttta aatcttacat
    23641 ctttgatttt tcaacacaga tagaatttaa ctttctccaa tacattcccg gtcataaaaa
    23701 aaacttcatc tccccctata tttttgtcgg agcttcggta tttaatttca atccgaaagc
    23761 tcaatacgaa ggcaactggt acgctttgca ttcgctgggc accgaaggtc agggaaccac
    23821 catcgaggga gttaaaaaac cctattcgct caccactttt gccatgccct tcggcctggg
    23881 cgtgaagtat tctccttaca aatggattag cctgggactg gaatatggca taagaaaaac
    23941 atttaccgat tatatcgatg atgtaagcac agtttatgcc gacccggtga ttttggccgc
    24001 tgaaaacggc gccgtggctg cagcgctggc cgacccatcc ctttccaaca cggtggaagg
    24061 gcacgattat accgggctga aacgcggtaa ttccaaaaca aaagactggt atgtatttac
    24121 cggccttacc gttacggtgc atatcaggac caaaaaagcc cgctgcgatg cctacaagca
    24181 acaccccaaa atcaaaatta aatacaagga ataatttatt ctcttccgag gaatctgcat
    24241 gaaagaaaaa attaatccta aaaaattgcc cgaacatgtt gcggttatca tggacggaaa
    24301 cgggcgctgg gctcaaaaaa gaggtgccat gcgtattttc ggccacgaaa atgctgtaac
    24361 cgctgtcagg gagaccgtgg aagccgccgc cgaactggga ataaagtacc ttaccctcta
    24421 cactttttct accgaaaact ggaacaggcc aaaaaccgaa gtggatgccc tgatgaacct
    24481 tttggtgaaa acaataaata atgaaaccaa aacactaatt gagaataata tccgtttaat
    24541 tgccatcggc gatttaacaa gcctgcaaaa agattgctac aacgaactga tgaacaccat
    24601 caacattacg tcagccaaca gccgcatgac actgatactc gccctgagtt acagtgcccg
    24661 ctgggaaatt accgaggccg ttaagcagat tgcacaacaa gttaaaaaca accaactgga
    24721 tgtcaacgat atcgatcagc ataccatttc ctccacactc tccacaaaag atatccccga
    24781 cccgtccctg ctgataagaa ccagcggcga ataccgtatc agcaactttt tgctttggca
    24841 actggcctat accgaactgt acttctcccc agtgttatgg cccgatttca gaaagaacga
    24901 tttatataat gctatcatag attttcagaa gagagaacga agatttggta tgactagcga
    24961 gcaaataaaa taacaagatg ctaaaaaaca ccttcaaact attaatactt ctttttttca
    25021 gcataccact acaagcacag atttctttag acgataaagg caatatcgac tatgccacgc
    25081 caaaagaata tgaaatacag aaaattgctt tttcgggcgt tcaatacacc gataccaata
    25141 tctgcaaatt gctcaccggc ttgtatgaag gacaaaaaat tatgattccc ggcgaaaaaa
    25201 catccaccgc catcaccaac ctgtggaatc agggattgtt tgataaagtc aggttgtatg
    25261 ctacgcgggt ggaagaaaac aaaatctggc tcgaaataga actcaaggaa catccccgcc
    25321 tggccgcaca gccgcttttc tcctccaatg taaaaaaatc cgaggtagat gatctgcgcg
    25381 aaaaaatcaa ccttaccaag ggcgatatca tcacgccaaa cgtcattcag cgctcgcaaa
    25441 atacgataaa aacttattat cacgacaagg gctattataa tgccgaaata aaaatcaaag
    25501 aaatcatcga caccgcctcc ggaaaatatt ccaccaaatt aaatattgac attaccagaa
    25561 acaaaaaagt caaggtccgc aaaatcaaca tcacgggcaa cgatcacgta tcggacttcc
    25621 gtctgaaacg gctgctgaaa aaaaccaaag aaaaaacagt gttcaaccct tttatgggct
    25681 gcgacaccct gctgttccaa tcggtaaaaa aactttttgt ccgcgacagc atcagtattg
    25741 aagacaattt tctcaattat atcaatgaaa gagtcaacct caggatattc aaatcagcca
    25801 aattcctgaa agacgaatac aacaaggaaa aactggccat cattgacaaa tacaatgaat
    25861 tgggctaccg cgattcaaaa attattgccg acaccattat ccgctcgggc gaaaataccg
    25921 tggatatcaa catcgacatc aaagaaggca ggcagtactt ttttcggaaa attacctgga
    25981 taggcaacac catttattcg gaacgcgaac tgaacgccat cctgaaagtc aaaaaaggag
    26041 acatctacaa ccagaaacaa ctggaaacca acttgagtta taaccccgaa ggctatgacg
    26101 tttcttcgct atacatggac aacggctacc tgtttttcag cgccaacccc atcgaaactg
    26161 ccgttgaaaa cgactctatc gatatggaaa ttcgcattat tgaaggcaag caggcataca
    26221 tcaacaaggt ttccatccgt ggcaacacca aaacaaaaga ccatgtggtc atcagggaaa
    26281 tacgttcaaa acccggacaa ttgttcaacc gttccgacat catccgcacc acgcgcgagc
    26341 tggcccagtt aaagtatttc aatgcggaaa aaattatccc cgacatcatc cccaaccctg
    26401 ccgacggcac agtggacatt gagtattcgg tggaagaaac ctcttccgac caactggaac
    26461 tctccggcgg atggggcatg ggccggttga tcggcaccct gggggtatca ttcaacaatt
    26521 tttccaccaa aaactttttc aaaaagtcgg catggtcgcc tttgccttcc ggcgacggac
    26581 aaaaattaag tatccgggcg caaaccaatg gaaaatatta ccaggcctat aatgcctcct
    26641 ttaccgaacc ctggctggga ggtcacaagc ctttggcatt aagcgtttcg gtatattatt
    26701 cttcgcaaac caacgggctt tccaaaagcg attcgaacag gcaggccata ggtattgccg
    26761 gcgcttcggt aggtttgggc tcgcgcctca aatggcccga cgactacttt accctctatc
    26821 acgagataag ttaccgcaac tacaaattga aaaactatta ttccactttt gactttaccg
    26881 atggcacttc caacaacctc aactacaccg taaccctatc gcgcaattct attgatgccc
    26941 ccatttatcc ccgttcgggt tcggaagttt cgctgatggc acaattgacg cctccttttt
    27001 cattattaaa tggcaaagat tactcaaaaa tggccgaaac agacaaatat caatggatag
    27061 aataccataa atggaaatta tctgcttcgg tttatacaaa attggcagga aatttggtat
    27121 tgagtgtgcg aacgaaattt ggatttcttg gttcttataa caaaaaagaa ctcggagtcc
    27181 cgccctttga aaggttttat ttaggtggtg acggattatc tggcttctcc cttgatggca
    27241 gagaactgat agcgctgagg ggatatacca ataattcact cacaccccgc aatagcaatg
    27301 gctatatagg cggctcgatc tttgacaaat ttactgttga actacgctac cctatttcgc
    27361 taaatcctat ggctactgtt tatgtactgg gttttgccga aggaggcaat gcctgggaca
    27421 aatggaaaga tttcaatccg tttgatattt acaggtcggc aggaataggt gtcagggtgt
    27481 ttttgccaat gttcggaatt ttgggattag actggggtta tggatttgat aatattccgg
    27541 gtgtgggcaa aggcggaagc cagttccatt tttcgatcaa ccagtcaatt gattaaccat
    27601 attattaaac aggaggttcg tatgaaaaag ttatttgttt tattatttgt gtttgcagct
    27661 tttacgttga attccaatgc acagaaattt gcctatgtgg attctgaata tattcttgaa
    27721 aatatgtcag aataccgcga cgctcaggaa ttgcttgaca agctttccct ggactggcaa
    27781 aaagaaatag aagccaaatt tgctgccatt gataaaatgt acaaagatta tcaggccgaa
    27841 gccgttttgt tgcccgaaga catcaagaaa aaacgtcagg atgagattat tgccaaagaa
    27901 aaagaagcca aagatcttca gaaaaaaaga tttggcaagg acggcgattt gtacaaaaaa
    27961 cgtcaggaac tcatcaagcc catacaggat aaggtgtacg acgccgttga aagcattgcc
    28021 aacgccagcg ggtatgctgt agttttcgac aaatcgggca gcaccaccat gttatatgcc
    28081 aactcaaaga atgaccgcag cgacgatgtg ctggaaaaac tcggatataa acccggtgct
    28141 atcaaaacaa atacgggcgg caaaaatcag gatgacgaag aataaaaaat tgtatctttg
    28201 cacccaagta aaaaatatta attaatcgta aatatgaaaa atttaacaaa aattgttctg
    28261 attattttcc tcgcggggct gaccacggtc tctttcgccc agaaaaaaca aaaattcggt
    28321 cacatcgact ccaatgaact gctgaaactc atgcccggac gcgattccgc cgaaattaaa
    28381 atcagggatt atgccaccac cctcgaaaac caactcaagg gaatgcaaag cgaactcgaa
    28441 accaaatacc agacctatct caccaacgaa gccaactata ccgacctgat aaaacaaacc
    28501 caacaaaaag aacttcagga tattcaggca cgtatcgaag cttttcaggc ctcagcccag
    28561 gatgatctgg aaaagaaaca gaacgaactg ctaaaaccca ttattgacaa agcaaaagcc
    28621 gctatcgaaa aagtaggaaa agataaccag tacacctata tttttgatgc cggaatcggt
    28681 gtacttctgt attctgaccc taccgaagac attttaccca tggtaaaagc cgaactcaat
    28741 cttaaataaa tctttgatgt cattgaaaaa aatcaaaaac tcaatactgg gtttttgatt
    28801 ttttttattt attaaagccg ctttgttgga taataaatat aaaatattat gaaacatttg
    28861 agcacctttt tactgaccac gttagtgatt gtcgttttca gcagcaccat aagagtgcag
    28921 gcacaaggaa aaaaccaggt acacatcaac ggagttataa aaaacaaccc gtttaccaat
    28981 gtttcgttgt atcagatggg ccgcgaaaac aagttcatcg ccgatacagt cattgctgcc
    29041 gacaatacat ttgcactggc gtgcaatgtg gacaaaacag acatgtataa ggtgcaattt
    29101 gccaataata attttgtgat cctggtgctg caacctggtg aaaaagttga tatctctatt
    29161 gatgccaacg actttttgaa gaaaatccag atcaaaggtt ccgaaacttc agcggatatt
    29221 taccggaatc aaaatttaat tacctcgcat aaggcgcgta tcgacagtat aaacaacctt
    29281 tcgcgaacag cggcagcaaa tcccaaatat gattctattt caaaggtgta cagggctgaa
    29341 acagaaaaaa ttaaaaagtc aatggatgaa aacttaaagg cctacattca aaaaaaaccg
    29401 gaatcgctgg ccatcttgtt tttaccggaa gtattgcccg tggacaacca tatagatgca
    29461 tacctgctgg ccgatgccgc gctgatggcc aaacatcccg ataatgtttt tgtacaaaac
    29521 ctgcacatgc aaatcaccaa tagccagaag accgccattg gcaccctggc gcctgatttt
    29581 tcgctgcctg acaccacagg aacccaaata gcgctaagtt ctttcagggg taaatacgtg
    29641 ctcattgatt tctgggcggc ctggtgtggc ccctgccggc gcgaaatgcc caacatgctg
    29701 aagttataca acgattttaa agataaaaat ttcaccattc ttggcgtttc tcttgataaa
    29761 acaagggcaa actgggtagg tgccatcagg tcggataaac tgccctggac gcaggtcagc
    29821 gaccttaaat tctggcagtc ggaagttgcc ggcctttacg atgtaaaagc aattccttat
    29881 accgtgctac tcgacaaaga gggcaagata gttgccaaaa acctccgggg agaagacctt
    29941 tatcaaaaaa ttgccgacct gctaaatcag tagtagtgac agcatcccct gacaacatca
    30001 tccaaaaaac cattgatttt gtaaagtcat cgtcatcggg cgaagcttcg gggcacgatt
    30061 ggtggcacat tttcagggtg cataatatgg ctgtgtacct tgcccgggaa gaaaaagctg
    30121 atctttttgt cgtggaactt gcggctttgc tgcacgacat tgacgactgg aaatttaatg
    30181 cccagggttc gggaaaggcc ttcgcttggc ttacgcagtg cggcctgcag gaaaacatca
    30241 tacagcaaat tcttgaggtg atagatacgg tttcatacaa aggggccggc gaacccaatc
    30301 atgccataag tattgaagca caagtggttc aggatgccga ccggctggat gctatcggcg
    30361 ccataggaat tgcccgcacc tttgcctttg gtggcagcca cggccgggaa atatataatc
    30421 cgcattgccc accggtgctg cacgcgaatt ttgaggccta caaaaaaaac caaagccaca
    30481 ccatcaacca tttctatgaa aaactgctgc tgctgaaaga ccggctcaat accaacagtg
    30541 ccaaagccct ggcggcagaa aggcatgctt ttctggaaaa gtttttagcg gcattttttg
    30601 ccgaatggaa tttcaatgcc ggaacggaaa ctcagaaata ataaaatagt gaataggtga
    30661 gccggatgcc tccaacgggt ttcacctcgc ttacctcgcc cagactgttg attttactgc
    30721 cataaccggc caccgaataa tcgccttcca gacccatttt tattttgccc gactgaaaca
    30781 caaaacgcgg cgaaacacga tagacatagg aaatattcca tccccgggca aaatacgaac
    30841 cggcatcctt ccaatcgagg atattgttat aagaccccag atttttggta aacccaccca
    30901 ggatgccaaa tttgaatttc gatccgttgg tggtaaaatc gagccagcca gccacggtgt
    30961 tcagaggggt gtagctaaaa tcaccgcggg caatggtgag gctgtcggta gtatgctgat
    31021 aggcatagcc tcccagcatg gtgaggttgt aaagatcctg tccatacacc ccggataatt
    31081 taaacgtaac tttcgggcat cggtatttaa aaaccagtga aaaggcatag gcattcagac
    31141 tgctgttggt ggcgtaattg ctgtcggtaa caatgcgggg caccaccacc ttgtaatcgg
    31201 cgcttccgcc gacaaatatt tcgcgggttt tatcctcgtt gaaatgctgg tacgaaatac
    31261 ggaggttcag ttcaggcaaa gccgcattgc gcagtatttc gggcccggcc cattcggcaa
    31321 aatcgcgctg cgacatgacc gacaaaataa acttaaagtc tttccaatgc tgggtaacct
    31381 tgatttgcgg gttacgcgaa aacggctgaa acggacaacc ggtatttaca tccatgattt
    31441 cgggaaaaca ttcttcaaaa aacattccat gccaggtttg tcccacagtg aggtcggtgg
    31501 tggcccacga aagcttggcg aaggcatgcc tcacgcggaa gcagttaatg ctggcatcgg
    31561 tggtaccata aaactccccc tctatcacgc cggaggtttt tgcgccgaat gcatcgggac
    31621 cggtaatatc gccccgcagt cggctttgta tggaaagaat gttgaaggtt gttttggcat
    31681 tgatatccac gctgtcggca tcgtaacgga tattttcggg aaaaagatgg aaatgtcctt
    31741 cccgaatacc gacagatttg cgggaatcgt aaaagaaatc atttttcaca tagccggaaa
    31801 aatgaatgcc gaatttatcg gttttttcct gtgcgtaggc cagcaaggga ataaatgtta
    31861 aagttaacag tataagtctt ttcatggaaa attcttttgt ttgttcagac aaatatatta
    31921 aagattgttt aagaaattgt tttgatttta tcttttaata tctttatgag tttttttgct
    31981 gtaacttctt tagatttttc agctcagtac aaacttcgtc tgcaaaatct gccacgtttc
    32041 gcaaaaaatc catcataatt cttaaataac ataaatcaat ttttctgggc gaaaaatagc
    32101 caccaatcat taaccataat tataataaca actcaacgcc aatgataaac aatataatga
    32161 attttaagaa actgaaaatc cttatgattg gattgattct tataacaacc ccatttttaa
    32221 gtatgggaca atcgaaacag gaaaaaacgg atacaattat taccaaattg aaactcattg
    32281 agggccagaa aatgaactat gaatttcaga tagaaccttt aaagtatcag gcaacaggga
    32341 aagacagcat tgaaattgtt gaactggaaa agaaattaac tgatgatgaa tttgttacac
    32401 tgatcagatc tgctattaat gaaacattta gcgaggatga aattaatgac atttattatt
    32461 ttattcaaac aagtgctttt gaaaagtttt tcaaatctgg
//
LOCUS       DAOSFP010000002        22585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_1832_length_22585_cov_4.340701, whole genome shotgun sequence.
ACCESSION   DAOSFP010000002 DAOSFP010000000
VERSION     DAOSFP010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_22585_cov_4.340701"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..890
                     /locus_tag="PLI16_00150"
     CDS             39..890
                     /locus_tag="PLI16_00150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH82998.1"
                     /translation="MGANTLYQLTNEIVYDYQAGDEFQYYDYYNNSSYPGTNYKRYKK
                     YKILNRTITADSLFYSAIYEWFNADSTNLFVDTIMLKYSRNTLIANIPFEKYNGQESR
                     TLGIKDYCGLRLWTFEKNRLSYYNEYSLCDTSWCYNSEPGPPVYYKSNYVSGLGLYLD
                     QSYYISPFPPNGNWGKELRMIYFKKNGISCGAEMFAGIKEYQDKDDKLIIGPNPAIGY
                     IHITSSVLLRSITLFTIEGQELLSQNLSGTAETIDVRNFHNGIFVARICFNDNTVAIK
                     KIVIFND"
     gene            974..1666
                     /locus_tag="PLI16_00155"
     CDS             974..1666
                     /locus_tag="PLI16_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH82999.1"
                     /translation="MIHYRQLLFIIIIFIPAIITAQTLEPTETNALLNVIVSSLDDKP
                     RTGELITLTGQANKKTFSGITDAKGKFSVLIPEGDTYTVKYKIFGGEEKFKQVTIPSQ
                     EGSLTFQLDIKYDPPKVFTLENVFFDTGKSTLRAESFKTLNDLAEVMKLKPTLIIEIA
                     GHTDNVGTPESNLKLSLNRANSVKNYLVNHGIATERISTKGYGDTQPVASNDSDDGRQ
                     KNRRTVVHILKE"
     gene            complement(1681..5157)
                     /locus_tag="PLI16_00160"
     CDS             complement(1681..5157)
                     /locus_tag="PLI16_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83000.1"
                     /translation="MFFFLAATGLYAQDFSNFRLKTLTPDNDTLLIDSLSLIPKSIII
                     TTAAGLPLDTSKYRIDYAKGVLIITDPVFFDNELKNKNLQITYRVFPFSLAATQRHKQ
                     PGIINRPVTGLDNPFLYKYENTTEDIFKWGTLNRSGNISRGISIGNNQDLVVNSALNL
                     QLSGYLSDDIQVLAAITDNNIPIQPDGNTQQLQDFDKVFIQLFNKNHKLIAGDFETGP
                     PKSYFMNFYKKAQGALIESNLLIPLKKHPEEKIKLKLEGSGALSKGRYARNVIAVLEG
                     NQGPYKLSGQNNENYIIILAGTERVYIDGVLLTRGQENDYVIDYNLGEVTFMPKNIIT
                     KDKRVVVEFEYTDRYYARTLFHVGAGMEYKKLQVRFNFFSEQDLKNQPLQQELTDSEK
                     MLLAGIGDSVQLAIVPNIDSIEFSSTYVMYKMIDTTVASVYYDSVFVYSTNPDSAFYR
                     LGFAFVGSGNGNYVLTQSSANGRVFKWVAPQGGQPGGDYEPVTLLIAPKKIQMYTLAF
                     DYGIGKTTKITAEAALSNNNKNLFSSLDKSDDVGYAIRTAVTNAVPFAKKSSNEWLFI
                     GDIANEWVSRHFSPIEQYRPVEFSRDWNVSKAVADYENISTLKIGVRNKKNQNAFYQV
                     KYYVQGDAYRGLQNQLDIAQDYKNFFLTFNGSYLATRTPSYKTAYFKQKAQLIKKFKW
                     FSVGVREEQEHNAFRTNSSDSLMGSSFAFNQYEAFFASPDSAKNKYQISYKKRFDYLP
                     LHNDLRLATDADDFNLGVELAKNPKNTLKISATYRMLKVKDSALSATRPDNRLIGRIE
                     YYVRLFKNVLMSTTYYEVGSGMEEKKEYSYLEVTPGQGVYVWSDYNNDGVKQLNEFDV
                     AVFKDQANYIRIFTPTYSYIKTYSNQFSEAITIDPGIVWKNKKGFRKFVSRFSLQSSY
                     SVNHKTTRPDFLHAYNPFMFQLNDTTLMSTATTFKTVLFFNKNHPKIAAEASYQLNNS
                     KMMLLNGFESRSQQSYGIKIRWNITRKFYTNIEFMQGIKTYGSEFFTSRNYDLSFWEI
                     QPKFIFQPGNKFRISLTYNFSDKRNTAVSPNERALNHNFGLESKYNFPGKGTLQAKVN
                     YININYNASDNTSLAYEMLEGLKNGQNITWGLTFQRNLGNNLQLDLVYDGRKTGTNKI
                     IHVGSVQVRAYF"
     gene            5380..6252
                     /locus_tag="PLI16_00165"
     CDS             5380..6252
                     /locus_tag="PLI16_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOH83001.1"
                     /translation="MKSMTGFGRAVCELPGKKITIEIKSVNSKQLDLNIKLPGLLREK
                     EADIRSLLSKQLERGKIDFYVNLEPLVSQGAFALNTAQAAQYHEGIKQLSQDLGLPMP
                     QEVISLLLRMPDVVKNPVEELKEEEWILLNQEIENAVSALDIFRRKEGAVLQKDILQH
                     ISIIEEGIPKIESYEKDRIQQIRERIGNNLSEFFDTKKIDQNRFEQELIYYLEKIDFT
                     EEKVRLQKHCTYFHETISEAASNGRKLGFITQEIGREINTLGSKANNSDIQKIVIEMK
                     DELEKIREQLLNVL"
     assembly_gap    6639..6738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6987..7928)
                     /locus_tag="PLI16_00170"
     CDS             complement(6987..7928)
                     /locus_tag="PLI16_00170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH83002.1"
                     /translation="MIFQPFLQDKKPYLKLLYFLIIVISCLFFINILGMLLGVPFFGK
                     SFVESMSGLNDYTDPVVIAKLKYLQVVNQLALFIVPALIFALFADNSVPGYLKLNRRI
                     KALPLLFAVCIIFAGLPLINWLSELNKNLSLSEKYSWLEQWMRDSETQASDITTAFLS
                     STSYGGLLINFLVIAILPALGEEFFFRGVLQRLFSEWFKNAHAGIIITALLFSTFHFQ
                     FFGFLPRFLLGMFLGYLFYWSGTLWLPIIVHFLNNGLAVAVAFLAAKGTANVSYETFG
                     SSDNILVILSSGIIVAGMAFALYKTRRSKNELQQPEA"
     gene            complement(8059..9054)
                     /locus_tag="PLI16_00175"
     CDS             complement(8059..9054)
                     /locus_tag="PLI16_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOH83003.1"
                     /translation="MKKNNKILQKIKSYRAFRIIRLITKKITLPGFDDMPLYDVVVFF
                     IKGLTDGAITTRASSVAYKFFIAMFPAIIFLFTLIPYIPIANFQDTLLDTIQSALPEN
                     VNSMINETITDIIARQHGGLLSLGFIFALYFASNGILGLITAFNATSHQIETRKWWQM
                     YAVSVLLVIILTLIVIISIALIMLGTTGINYLISAHIIEESVLLFIIRFSKWIIIFLM
                     IFFVVSFLYYLGPVKRKEFRFISAGSTLATLLFIVTTIGFKFYVENFGSYNAVYGSIG
                     TVIVILMWLYLNSLALLVGFELNASILEAKKDKNVMAIIARKPKKQKPRKQKTKS"
     gene            complement(9051..9890)
                     /gene="nadC"
                     /locus_tag="PLI16_00180"
     CDS             complement(9051..9890)
                     /gene="nadC"
                     /locus_tag="PLI16_00180"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOH83004.1"
                     /translation="MNDISHLIRQAIAEDIGDGDHTSMATIPEDATGKMKLLVKEDGI
                     VAGVEIAKLVFEKIDPGLCMEVLITDGMPIKKGDIVFYITGRCRSMLIAERTVLNFMQ
                     RLSGIATSTSHYVRELEGTSARLLDTRKTTPNMRIFEKMAVVLGGGMNHRMGLYDMVM
                     IKDNHVDFAGGIKQAIEAEHDYLNRIGKKLKIEIEVRNFDELEQVIKVGRVVRIMLDN
                     FPPADLQKAVEIIGGKFETEASGGIKLENIREYALTGVDFISVGAVTHQIKSLDLSLK
                     AVK"
     gene            complement(9956..10390)
                     /locus_tag="PLI16_00185"
     CDS             complement(9956..10390)
                     /locus_tag="PLI16_00185"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOH83005.1"
                     /translation="MRHSLIIVVFCLITSSVFAGQQVVPSTGDTTLALVQDDITSAVA
                     AAINAGDAKKLASFFSPSIDLTLPGTEGTYSKSQAEMIVKDFFTKYPPVSFKINQQGS
                     STEGSQFAVGTMVSKAATFKTYFLIKKRDGLPLVHQLQFEQE"
     gene            10523..10996
                     /locus_tag="PLI16_00190"
     CDS             10523..10996
                     /locus_tag="PLI16_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOH83006.1"
                     /translation="MKITLLLTGKTEVAPVKELMEDYAKRLGHYINFEIIATPELKNI
                     KNLSIEQYKQKESEIQKKYLAKSDFIVLLDEYGKEYTSEKFAQYLEQKTGIGIKNLMF
                     VVGGAYGFADEIKQMAHGCLSLSKMTFPHQLVRVIFLEQLYRAFTIIRKEKYHHS"
     gene            complement(10987..12630)
                     /gene="lnt"
                     /locus_tag="PLI16_00195"
     CDS             complement(10987..12630)
                     /gene="lnt"
                     /locus_tag="PLI16_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOH83007.1"
                     /translation="MKKGHLVLLSILSGLLLSLAWPARGFPFLLFFAFVPLLYIEDYI
                     AKNRHNFIKSSVFFYAYIAFFIWNGLTTWWIYYSTTFGVAMAVALNSLFMAITFLAYH
                     LIRRTVFKSRGGYYVLILLWISFEFLHLDWDLSWSWLNLGNGFANYPRFVQWYEFTGV
                     FGGDIWILLFNILFYKLLTSLFGRFKSRKDVYLFGTSSVLLMAIPIALSLTMYYSYSE
                     KEAPYNCVVVQPNVDPYGKFTSITIEEQLNSILGLAKQKVDDNTDFLVCPETALPEYI
                     NEDEINESASIDSLKSLISKHPKLKIVIGISSFKIFKEGEKLSRTARYSERGKYYYDV
                     CNTALYLDSAFVPQLYHKSKLVPGVEMMPFPKLLKPLEKFAIDLGGMTGSNGTQEERT
                     AFGAKDSPCKVAPVICYESIYGDFITGYIRNGANLIFIITNDGWWKNTAGHRQHFQYA
                     RLRAIETRRSIARAANTGISAYINQKGDVFMPTAYWTPEVMTMAINTNTRMTFYARYG
                     DYLGKIALYMSAALVVVFFAAILLNKRKRLRIKKKNNQE"
     gene            complement(12676..14100)
                     /locus_tag="PLI16_00200"
     CDS             complement(12676..14100)
                     /locus_tag="PLI16_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83008.1"
                     /translation="MINPPKTHFFQDPGKTFVFFSALAVIIILLSANLPFFWDNITIS
                     EYINFYLRDSFRHIIPPLETDFGPLTINALYLAACCAIFGKTLLVCHLAIIPVVVGIF
                     LEIKKISLRFIKPELLPMVYLLLLFDPAFITQIILMGYDLFLLYFVLLAIRSLLEKKH
                     LLYSLSLLLLAATSFRALFFIPALALIHFMVLLLYDKTRPQIKDVLVYLPAMFFLISW
                     SIIHYLVTGWIIINPANVVHISMNGPAMVIRQFIYILWKITDSGKILPWLFCILCILV
                     YYRKLRTHKLKLLLWLLFAPLAVNSLIMIFLSNPVGHKYFLQSFVFLIILTVYFLQQI
                     HLKKIRLGIFLLILAGFFAGNFIFYPQKYGNAWETSLKVLPYFSLEKDLAAYVKHQGI
                     RPGDIYSFYPVTNNQALSWLDEDFAYADASHANMDTCRYVITSNVYNIKNPEAIHAKA
                     QQWIPLKHFSRGQVYLKLYKNPAR"
     gene            complement(14093..15025)
                     /locus_tag="PLI16_00205"
     CDS             complement(14093..15025)
                     /locus_tag="PLI16_00205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH83009.1"
                     /translation="MPAPEISIVIPVYNSSSILPELSRRISAALSNLPHEVIFVNDCS
                     HDDSWEVLKSLTAANTNYTAIDLVKNSGQDNAILAGLRHIKGNYAVIMDDDLQHAPED
                     IPALYEKCKEGYDVCYALYEKKKHNFLKKIGSKINGRIARLILNKPKGIYLSPFKIIK
                     HSIVNEINRFSGPYPYIDGILLTITHNICQIKVTHYKRLTGKGNYNLFRSFSVLFRLF
                     SGFSVVPLRIMTLVGLAIVLFGLGLIIYYLYEFYVLKDFVEGWTTIVVLVIFFGGMTT
                     MLLGLIGEYIGRIYLTLNNKPQYLIKKIIKSSND"
     gene            complement(15007..15639)
                     /locus_tag="PLI16_00210"
     CDS             complement(15007..15639)
                     /locus_tag="PLI16_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83010.1"
                     /translation="METNTHIMPPGIIYRNILLYRLAMNLLYSFSYKERFRKVTSYIT
                     GKSVNELCFGDLFIASFCKKNNICWTGFEVNKTFVRRAIRNNFRAQQIDLKNQEITLP
                     PADTNIITGSLYHFHEQPEKILNALFTTANRIIISEPVKNISAKKNLWGRLAAKVSDA
                     GNGPEEFRYIENDLLKTIEVSGKKFNFTTYKTEHFKKDIIIVLDKCQLPK"
     gene            complement(15629..20581)
                     /locus_tag="PLI16_00215"
     CDS             complement(15629..20581)
                     /locus_tag="PLI16_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOH83011.1"
                     /translation="MKRLLSVLFLFSLCLPAFAQPYGNEWINYSQSYYKVYIHENGVY
                     RIDYNTLLSNGFPLGSVNPRNIQVFNKGIEQYIYIKGENDNIFNPGDYIEFYAEKNDG
                     TLDTPLYKNPEGQANKAYSLFSDSSVYFITWNNSFSNRRLQSDNDVNFSSYSPVPYIF
                     NTVRQNYTSAYYAGETNSGGITDPEYTANEGWFDNGFNVGQSVTKNISSANKYANGPD
                     AEVDFVVLGQSNYAGVYIDHHLNINFAGITIDTLFDGYQVLRYNYTIPVAQIGNTSTP
                     FVFSSVNDLGVGSDRNTIAYIQIKYPHTLNLGGVSVFDFSAMDALQAKSLLQFTNFNI
                     PSGDTARLYDITNHRRIKVVYDGTAFKCLLSNSGGEKFCHLTSDNNIKPINKIYPVSY
                     DAKFINYQTIANTEFTDYLILTHPSLWAEAENYESYRNTTGYHALTVNVEHLYDQFGY
                     GVLKSAFAIRNFARFAFYHFAEKPRGLFLVGKGYRAASDGSLPQYRTDAYHNQLTLVP
                     SFGYPPSDLLFSQGIIDSLFMPAIPTGRLSARTPEDVAIYLDKVQQFELAQQTPQEWM
                     KTILHFGGGGNLSEQNMLAAYLNHYKHTIEDPLFGGHVRTFLKTSSEPMQINQSDSLR
                     NLINNGVTLMTFFGHAASVGFDVSVDVPSEYQNYGKYPFILANSCWAGDIYLDQNSTS
                     EEFVLIEDKGSIGFLASISLGIPAALNVFSDELYHQISYKNYGKPIGEIIKNTIDSIQ
                     GPDLIIKENTLTMVLHGDPAIILNSFAKPDYAITPPNVFFTPPQVTSDLDSFNIHIVS
                     TNIGRAINDSIIVQTTRTFPGGITPTNYFNKIKAPLFKDTLTLRVPVDIINGLGLNKF
                     RIYLDCYQTIDEISEINNITEVPLMIKSSDIVPVYPYKYAVMPALPLTLKASTSDAFI
                     GSQDYEFQLDTSDAFNSPIKQVFHINHSGGVVPWTPNFPITTDSIVYFWRVSIDSSDQ
                     HSYNWRESSFQYINGKRGWGQAHFHQFKNDRYRYVKYNKPQRRFDFVNDVKSLTCQTG
                     FYPYIPWQDEYYKVNNVMKFQWAYMGPGGNGMILAVIDSVSGEPWMNYAMPQPETEAG
                     WEFPCNTFAEREAMRSFINNVPDGNYILAYSHRNHYAEDYSEDQYQSFESFGSSQIRT
                     LPNTIPYIIFGKKGGAIGSAHELQGSAITSIVKLYDSVTTKWYEGYIESELIGPASSW
                     GSLHWRKKPFEPTNTDSAKLFVLGIKFNGQTDTLIQNLPPDSADIYNLNTRINASVYP
                     YLKLLVSMRDDSLHTPAQMRRWQVLYEGVPETALNPSLHFAFFNDTIAEGENILFSTA
                     TQNISDYDMDSLLIKYWVVDHNMITHPLGSFRHRPHPSGDILTDTITFSTNGLSGLNS
                     LWVEVNPDFDQIEQTHFNNIGEIKFLVDRDRINPMLDVTFDGIHIFDGDIVSAKPEIQ
                     ISLKDENKFLALNDTACFRVYLQVPGNNTRERVYFMKQGQEILQFIPASLPNNSCRII
                     YNPDFLQDGVYQFFVEARDISHNESGSNEYAISFEVVNKPSITHVMNWPNPFNNATHF
                     VFTLTGSEVPTYFKIQIMNISGKVVREIDLAELGPIHIGRNITEYAWNGTDEFGDRLA
                     NGVYLYRVVTRLNDETLELPKTDADQYFKKEFGKMYLIGN"
     gene            complement(20587..21711)
                     /locus_tag="PLI16_00220"
     CDS             complement(20587..21711)
                     /locus_tag="PLI16_00220"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOH83012.1"
                     /translation="MIRKFFTITIVCFIGMGMVHAQAPKYSNEFLSIGVGARALGMSN
                     SNVSFVNDVTSGYWNPAGLAMVPSKFQVGLMHSEYFAGIAKYDYGALAVQIDDKSTAA
                     LTFIRFGVDDIPNTTELIDAQGNIDYDRVTTFSAADYAILLSYARKSKIEGLRYGGSA
                     KIIYRKVGSFAHSWGFGLDIGVQYDLKKWHFGATARDITSTFNAWKYNLDDNMKDVFT
                     RTGNEIPENSLELTLPRLNLGAARRFDFPKKFSALVAIDLDMTFDGKRNVLIRSNPIS
                     IDPHGGLEFGYNDMVFLRGGFGNIQKETNIDGKKKISFQPNIGVGIVIKKIVAIDYAF
                     TDVGNASIALYSHVFSVKLNINPKKTAVEKNQEKQKNSFF"
     gene            complement(21841..>22585)
                     /locus_tag="PLI16_00225"
     CDS             complement(21841..>22585)
                     /locus_tag="PLI16_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH83013.1"
                     /translation="DMMQAQIDLNLAEQNFRSQQLVIENSKTELLQLMGGSQEQQFIT
                     GDSLITVDPQITLDSVLLFLKDNYQYLTAEQQIKISEQLAKEARAERMPSLRLNTAYE
                     VGFAKNTKGNTLTSLNYGPYAGLNLQIPIFNGGTYRVRQKTAMLNTSTARLEKENLFN
                     ELTSSARKTHQAYKNALDQLDIQQASFQLSEKLVALVLQKFQLNQATILDLKSAQQSY
                     ENSAYLLVNLQYQAKVAEIELKRLSCLLK"
BASE COUNT         6291 a         4518 c         5288 g         6388 t
ORIGIN      
        1 aagttataga tttttatcta taatcttgtc cgactatttt gggggctaat acattatatc
       61 aacttaccaa cgaaatagta tatgattacc aggccgggga tgaatttcaa tattatgatt
      121 attacaacaa ttcctcatat ccgggaacaa attataaacg gtataaaaaa tataaaatac
      181 tgaacaggac tataactgct gattcgcttt tttatagtgc gatttatgaa tggttcaatg
      241 cggactccac gaatttattt gtggatacca ttatgttaaa atattcccgt aatacactga
      301 tagccaatat tcctttcgaa aaatataacg gtcaggaatc aagaactctt ggcataaaag
      361 attactgtgg gctaagatta tggacatttg aaaaaaacag gttgtcgtat tataacgaat
      421 attcactgtg tgataccagc tggtgctata atagtgaacc gggacctccg gtatactata
      481 aaagtaatta tgtttcaggt cttggtttat atcttgacca gtcatattat atatcgcctt
      541 ttccccctaa tggaaattgg ggcaaagaat tgagaatgat ttattttaaa aagaacggaa
      601 tatcctgtgg tgctgaaatg tttgccggaa tcaaagagta ccaggacaaa gacgataaat
      661 tgattattgg tcccaatccc gccataggct atattcatat tacgtcatct gtattactta
      721 ggagtattac tttgtttacc attgaaggtc aggaattgtt atcacaaaac ctttccggaa
      781 cggctgaaac aatagatgtc aggaattttc ataatgggat tttcgttgca agaatttgct
      841 ttaacgataa tacggttgct ataaaaaaga tagttatttt caatgattaa agtgttcgct
      901 atataacata actcagcata ataataatta attaataatg atgtaacgtt tttaatcata
      961 aaaaacatcc aacatgattc attatcgcca attacttttt attattatca tttttatacc
     1021 ggccattatc actgcccaga cactcgaacc caccgaaacc aatgcattgc taaatgtgat
     1081 tgtcagcagc ctggatgaca aaccaagaac cggcgaactg atcaccctga ccggacaagc
     1141 gaacaagaaa actttttcgg gaattacaga cgcaaaagga aaattctcgg tgttaattcc
     1201 tgaaggggat acgtatactg taaaatacaa aatctttgga ggtgaagaaa aatttaaaca
     1261 ggtcaccatt ccaagccagg aggggagtct cactttccag cttgatataa aatatgaccc
     1321 gcccaaagtg tttacgctcg aaaatgtgtt ttttgatacc ggaaaatcta cacttcgcgc
     1381 cgaatcattt aaaactctca atgatctggc agaggtgatg aaacttaaac ccacacttat
     1441 cattgaaata gccggccata ccgataatgt cggcacaccc gaatccaacc tgaagctttc
     1501 gcttaaccgc gccaattcgg taaaaaatta cctggtaaat catggtattg caactgaacg
     1561 aatctcaaca aaagggtatg gcgacacaca acctgtggct tccaatgatt ccgatgacgg
     1621 ccgccagaaa aaccgccgga ctgttgttca tattttaaaa gaataacgcc ttttattaaa
     1681 ttaaaaatac gcccttacct gcacactgcc tacatgaatt atcttattag tgccggtttt
     1741 ccggccatca taaacaaggt cgagttgcag gttattaccg aggttgcgtt gaaaagtcaa
     1801 tccccaggtg atattctggc cgtttttcag tccttccagc atctcgtatg ctaatgaagt
     1861 gttgtccgaa gcattatagt tgatgttgat ataatttacc ttggcctgca gggtaccttt
     1921 gccagggaag ttgtatttgc tctccaggcc aaaattgtgg ttcagggcac gttcattggg
     1981 cgatacagcg gtgttgcgtt tatcggaaaa gttgtaggtg aggctgatgc ggaatttatt
     2041 gcccggctga aaaataaatt tcggctgtat ctcccaaaaa gataaatcgt agtttctgga
     2101 ggtgaaaaac tccgagccat aagtctttat cccctgcatg aattcaatgt tcgtgtaaaa
     2161 tttacgggta atattccatc ggatttttat cccgtatgat tgttgtgacc ggctttcaaa
     2221 gccattgagc agcatcattt tggagttgtt aagctggtac gaagcttcgg cagcaatttt
     2281 aggatgattt ttgttgaaaa acaaaaccgt tttaaacgtg gtggctgttg acatcagcgt
     2341 ggtgtcgttc aactgaaaca taaaaggatt gtatgcatgc aggaaatccg gccgtgtggt
     2401 tttgtggttt acgctgtacg acgactgcag cgaaaagcgt gaaacaaact tccggaaccc
     2461 ttttttattt ttccacacta tgcccggatc gatggtgatg gcctcgctaa actggtttga
     2521 ataagttttg atatagctgt atgtaggcgt aaatatccgt atgtagttag cctgatcctt
     2581 gaataccgcc acatcaaatt cgttgagttg ttttactccg tcgttgttgt aatcgctcca
     2641 cacatacacg ccttgtccgg gggtcacctc caggtaggag tattcttttt tttcttccat
     2701 gcctgagcct acttcataat aggtggtgga catcaataca tttttgaata agcgtacata
     2761 atattctatc cggcctatca gccggttgtc gggtctggtg gcgcttaggg cagagtcttt
     2821 taccttcagc atcctgtagg tggcgctgat ttttaaggtg ttttttgggt tttttgccag
     2881 ttctaccccc aggttgaaat cgtcggcgtc ggtggcaagg cgcagatcgt tatgtaaggg
     2941 caggtaatca aaacgttttt tgtaacttat ctggtatttg ttctttgccg aatcgggact
     3001 tgcaaaaaaa gcttcgtact ggttaaaggc aaaactgctt cccatcaacg aatcggaaga
     3061 gttggtgcgg aatgcgttat gctcctgctc ttcgcgcacc ccaaccgaaa accatttgaa
     3121 cttttttatt agttgcgctt tctgtttaaa ataggcggtc ttataggacg gagttcgggt
     3181 agccagatag ctgccattaa aagtcaggaa aaagtttttg taatcctgtg ctatgtcgag
     3241 ctggttttga aggccccggt atgcatctcc ctgaacataa tattttacct gataaaaggc
     3301 attttggttt tttttgttgc gaacaccaat ttttaatgtg ctgatgtttt cgtaatccgc
     3361 cactgccttg ctgacgttcc agtcgcggct gaattctaca ggccggtatt gttcgatggg
     3421 cgaaaaatgc cggctcaccc attcgttagc aatatcgccg ataaacagcc actcattgct
     3481 gctctttttt gcaaaaggca cggcattggt tacagctgtt ctgatggcat agccaacatc
     3541 atcactttta tcaagtgacg aaaacaggtt tttgttgttg ttgctcaggg ccgcttcagc
     3601 agtgattttt gttgtttttc cgatgccgta gtcaaaggcc agggtgtaca tctgtatctt
     3661 tttgggtgct atcagcaggg ttaccggttc gtaatcacca ccgggctggc cgccttgcgg
     3721 agccacccat ttgaacactc ttccgttggc cgaactttgt gtgagcacat aattgccgtt
     3781 tccactgccc acaaaggcaa aaccaagccg gtaaaaggca ctgtcgggat tggtgctgta
     3841 aacaaacacg gaatcataat atacggaagc aaccgtggtg tcaatcattt tatacatcac
     3901 atacgtactc gaaaattcta tgctgtcgat gttggggact atcgccagtt gcacactgtc
     3961 gccaatgcca gccagcagca ttttttcgct gtcggtaagc tcttgctgaa ggggctggtt
     4021 tttcaggtct tgttccgaaa aaaaattaaa ccgcacctgc agttttttat actccatgcc
     4081 tgcccccaca tgaaaaagtg tgcgcgcata ataccggtcg gtatattcaa attcaaccac
     4141 cacccgtttg tctttggtaa tgatattttt gggcataaaa gtaacttccc caagattgta
     4201 gtctatcaca taatcgtttt cctgtccgcg cgtaagtaac actccgtcaa tataaacccg
     4261 ttcggtgccc gcaagaatga tgatatagtt ttcgttgttc tgaccgctga gtttgtaagg
     4321 tccctgattg ccttccagca cagcgattac gttccgcgca taccttcctt tcgacaaagc
     4381 cccgctgcct tcaagtttca atttaatttt ctcttcggga tgttttttta aaggaattag
     4441 caagttcgac tcgataaggg cgccctgggc ttttttgtag aagttcataa aatagctttt
     4501 cggtggccct gtctcaaaat cgccggcgat cagcttatgg tttttgttga acaactgaat
     4561 gaaaaccttg tcgaaatcct gcaattgctg tgtgttgcca tcgggttgta tggggatgtt
     4621 gttgtcggtt attgctgcca gaacctgtat gtcgtcagac aggtagcccg aaagctgaag
     4681 attcagcgcg gagttgacca ccaggtcctg attgttgcct atggatattc cccgcgagat
     4741 attgccgctg cggttcagcg taccccattt gaatatgtct tctgttgtgt tttcatattt
     4801 atacaaaaaa gggttatcca atccggtaac aggacggttg atgataccag gctgcttatg
     4861 ccgctgcgta gctgccagcg aaaaaggaaa aacccggtaa gttatttgca ggtttttgtt
     4921 ttttagttca ttatcaaaaa aaaccggatc ggtaatgata agcactcctt tggcataatc
     4981 aatgcggtat tttgaagtgt ctagaggtag tcctgctgcc gtggtgataa taatactttt
     5041 gggtatcaga cttagtgaat caatgagcag agtatcgtta tcgggggtaa gggttttaag
     5101 cctgaaattc gagaaatcct gtgcatacaa tcctgttgcg gccagaaaaa aaaacaaaaa
     5161 tatgtaaagg atttttctca aaatttatac ctaaggatat tgcgaattta tcaataaaac
     5221 gggtattttt taaaactcaa atttatgata aaaattgtaa cctttcatgg tgcttgtttt
     5281 gattaactgc ggttgctggc cgttatgaca gcaggcggca aaaatcaggc aaacaattcc
     5341 ggaaaatgcc tacatttgta cgtagaaaaa caacacctca tgaaatcaat gaccggtttc
     5401 ggcagggcag tgtgtgaatt gcccgggaaa aaaattacta ttgaaataaa atctgttaac
     5461 agcaaacaac ttgacctgaa cataaaactg ccgggcttgc ttcgcgaaaa ggaagccgat
     5521 atcaggtcgt tgttatcaaa acaattggaa cgcggcaaaa tcgattttta tgtgaatctt
     5581 gagcctcttg tttcccaggg ggcatttgca ctgaacactg cgcaggcagc ccaataccac
     5641 gaggggataa aacaattgtc ccaagacttg ggtttgccaa tgcctcagga ggtgatatcg
     5701 cttttgttgc gcatgcccga tgtggtaaaa aatcctgttg aggaacttaa agaagaagag
     5761 tggatactac tgaatcagga gattgaaaat gcggtttctg cgctggatat ttttcgccgg
     5821 aaagaagggg ccgtactcca aaaagatata cttcagcata ttagtatcat cgaagaagga
     5881 atacccaaaa tagaaagtta cgaaaaggac cgcatacaac agatacggga gcgtataggc
     5941 aacaatcttt cagagttttt tgacacgaaa aaaattgatc aaaaccgttt tgaacaggag
     6001 ctgatttatt atctggagaa gatagatttt acagaagaaa aagtccgttt acagaaacat
     6061 tgtacctatt ttcatgaaac catcagcgaa gccgcatcaa acggccgaaa gcttgggttt
     6121 atcacgcagg aaataggccg cgagattaac accctgggat caaaagccaa caactctgat
     6181 attcagaaaa tagtcatcga aatgaaagat gaactggaga aaatccggga acagttgctt
     6241 aatgtccttt aaaaattata tccgcgaaaa tcagcgcgat cagcgggaga acaaagaaaa
     6301 atagttcccg cagataacgc agataaacgc agaaaaacaa aaataaatct gcgaaaatca
     6361 acgagattag aggggaaaaa aagaaagtga ttcccgcaga taccgcagat tatcgcagaa
     6421 gaaatcaata aaatcctccg tgtcgcgcca tgctctccat gtctccgtgg ttaatttata
     6481 ccaattagaa attaaaaata tgcacgggct ttatggtata atgccgatgt aacagatgtt
     6541 atagaccaat tcgttggact ataacatatt tgcaatcggt ttaaatctgg gaaaatcagc
     6601 gcgattagcg ggagaaaaaa ggagaatggt tcccgcagnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnaa tctgggaaaa tcagcgcgat tagcgggaga aaaaaggaga
     6781 atggttcccg cagataatgc agataagcgc agaaaaataa aaaaataatc tgcgtaaatc
     6841 agcgcgatca gcgggaaata aaaaggcgga gactcccgca gataaagcag attggcgcac
     6901 aagaaatcaa caaaatcctc cgtgcccctc cgtgtccgcc gcggcggatc cgtgtctctg
     6961 tggttaattt atccctgaca aaaatctcaa gcctccggtt gctgaagttc gtttttcgac
     7021 cttcttgttt tgtatagcgc aaaagccatc cctgcaacaa taatcccgct gctgaggata
     7081 acaagaatat tatcggaact gccaaaagtt tcatagctca cgtttgccgt tccttttgcc
     7141 gccaaaaaag ctactgccac cgccaggccg ttgttcagga aatgcacgat gataggcagc
     7201 cataaggtgc ccgaccagta aaataaatat cctaaaaaca tccccaacag aaaacgaggt
     7261 aagaagccaa aaaactgaaa atgaaaagtg ctgaataaca gggcggtgat gatgatgccg
     7321 gcatgggcgt ttttaaacca ctccgaaaac agccgttgca acactcctct gaaaaaaaac
     7381 tcttcgccca gcgccggcag tatagctatt accagaaaat ttattagtaa gccgccatag
     7441 ctggtggaac ttaaaaaggc agtggtgatg tcggaagcct gggtttcgga atcccgcatc
     7501 cattgttcga gccacgaata cttttctgac aggcttaagt ttttgtttaa ctctgaaagc
     7561 cagttgatta aaggaagtcc cgcaaaaata atgcaaacgg caaacagcag cggcagggct
     7621 tttatccttc tgttcagttt caggtaccct ggtacgctat tatctgcaaa caatgcgaat
     7681 atcagcgccg ggacaataaa caaagcaagc tggttgacca cctgcagata tttcagtttg
     7741 gcaatcacca caggatctgt ataatcattc aggccagaca tggattccac aaaggatttg
     7801 ccaaaaaatg gcacgcctag caacattccc agaatattaa taaaaaaaag gcaactgatt
     7861 actattatca aaaagtacag aagtttcaga tagggctttt tatcctgcag gaaaggttga
     7921 aaaatcatat gttgtgtaat gggtttaaaa ataatttgga aacagttttt tgatggtttt
     7981 tgtcgttggg tttgcttctg taacatcaac agatagtagg acttactgtt attgttgttt
     8041 taccggcatg cagccttatt atgattttgt tttttgtttt ctgggttttt gtttttttgg
     8101 tttccgggcg atgatggcca tcacattttt atcttttttt gcctcgagta tgctggcgtt
     8161 taattcaaaa cccaccaaaa gtgccagcga gtttaaatac agccacatca ggatcacaat
     8221 aacggttcct atagatccat aaacggcatt gtaactgcca aaattttcca cataaaactt
     8281 aaaacctata gtagtaacaa taaacaaaag ggtggccagc gtgctccctg ccgaaataaa
     8341 acgaaactct tttcttttta ccgggccaag gtagtataga aaagaaacca caaagaaaat
     8401 catcagaaaa ataattatcc atttcgaaaa tctgatgata aataacaata cgctttcctc
     8461 aatgatgtgt gccgaaataa ggtaattaat accggtagtg ccaagcatga ttaacgctat
     8521 ggagatgatt actatcagcg taaggatgat taccaaaagc acggaaacag catacatttg
     8581 ccaccatttg cgggtttcaa tctggtgcga tgtggcatta aaggcggtta taaggccaag
     8641 tattccgttg gaagcaaaat acagggcaaa aatgaaccca agtgaaagca accctccgtg
     8701 ttgacgcgca atgatatcgg tgatggtttc gttgatcatg ctgttgacat tttcgggcag
     8761 ggccgactga atggtgtcca gcagggtgtc ctggaagttg gcaatgggaa tatacggaat
     8821 cagcgtaaac aaaaaaatga tagccggaaa catggcgata aaaaatttgt aagccacgga
     8881 gctggcccgc gtagtgatgg cgccatccgt cagtcctttt ataaaaaaaa ccactacatc
     8941 atacagcggc atatcatcaa agccaggcag ggttattttt tttgttatga gcctgatgat
     9001 tctgaaagcc cggtaggact tgatcttttg caggattttg ttgttttttt tcatttaaca
     9061 gctttcaggc tgaggtcgag actttttatc tgatgagtaa cggcgcccac cgatataaaa
     9121 tctactccgg taagggcata ttcgcggatg ttctccagtt taatacctcc cgaggcttcg
     9181 gtttcaaatt ttccacctat gatttccacg gctttttgca ggtcggcagg agggaaattg
     9241 tcgagcatga tacggaccac ccggcccacc tttattacct gctccagttc gtcgaagttg
     9301 cggacttcaa tttctatttt cagctttttt ccgatacggt tcaggtaatc atgttcggct
     9361 tctatggctt gtttgatgcc tccggcaaaa tccacatgat tgtcttttat catcaccatg
     9421 tcgtacagcc ccatgcggtg gttcattccg ccgccgagga ctacggccat tttttcaaaa
     9481 atccgcatat tgggcgtggt cttgcgggtg tcgagcagtc ttgcactggt gccttcgagt
     9541 tcgcggacat agtggctggt ggatgtggca atgccgctga ggcgctgcat gaagttgagc
     9601 acagtgcgtt ctgcaataag catcgaacgg caacggcccg tgatataaaa aacgatatcg
     9661 ccttttttta tcggcatgcc atcggttatc agcacttcca tgcacagtcc ggggtctatt
     9721 ttttcaaaaa ctagttttgc aatttccaca ccggccacta tgccgtcttc cttgaccaac
     9781 agcttcattt ttcctgttgc atcctcaggt atggtggcca tggatgtgtg gtcgccatcg
     9841 ccaatatctt cggcaatggc ctgccttata agatgtgaaa tgtcattcat ctgtaataat
     9901 taagcgtttg cgctaaatta caaaaaattg gggcagataa tgtataatca gaaaactatt
     9961 cctgttcgaa ttgtagctga tgcaccagcg gaaggccatc cctttttttg ataagaaaat
    10021 atgttttgaa cgtagcggcc ttggagacca tagtgccaac ggcaaactgc gaaccttcgg
    10081 ttgatgagcc ctgttggttt atcttaaagg ataccggcgg gtatttggta aagaaatctt
    10141 tgacgatcat ttcggcttgc gatttgctgt aggtgccttc ggtgccggga agggtaaggt
    10201 cgatactggg gctgaaaaac gatgccagtt tttttgcatc accggcattg atagctgctg
    10261 ccacggcgga agtgatgtcg tcctgaacaa gagcaagggt ggtgtcgcct gttgacggaa
    10321 caacctgttg accggcaaaa acactgctgg tgattaaaca aaaaactact ataattaatg
    10381 aatgtcgcat tattatgatt ttttatattt attgcaaaaa ccaagccaca ataacttcat
    10441 gaaaaaacag taacaaagtt ataaactttt ggcaaaggtt tttaatttta taattgtata
    10501 attttaccac agaatttata tcatgaaaat tacattgctt ttgacaggaa agacggaggt
    10561 ggctccggtc aaggaattga tggaagacta tgccaaaagg ttgggccatt atattaattt
    10621 tgagataatt gccactccgg agctcaagaa tattaaaaac ctttccattg aacaatacaa
    10681 acaaaaagaa tccgaaatac aaaaaaaata tctggcaaaa tccgatttta ttgtactgtt
    10741 ggatgaatat ggtaaagaat acacctccga aaaatttgcg cagtatttag aacagaagac
    10801 cgggataggc ataaaaaacc tgatgtttgt ggttggaggc gcctatggct ttgctgatga
    10861 aataaaacag atggctcatg gctgcctgtc gctttcaaaa atgacgtttc cgcatcagtt
    10921 ggtgcgggta atttttcttg agcagttata cagggctttt accatcatcc gaaaagaaaa
    10981 atatcatcat tcctgattgt tttttttctt tattctcaat cttttgcgct tgttgagaag
    11041 gatggccgcg aaaaacacta ctaccagcgc tgcagacata taaagggcaa tctttcccag
    11101 atagtcgcca tagcgggcat aaaatgtcat gcgggtattg gtgttgatgg ccatggtcat
    11161 cacctccgga gtccagtagg cggtaggcat aaagacatct cccttttggt tgatataggc
    11221 tgagatgccg gtgttggcag cacgggctat gctccggcga gtttcaatag cgcgcaggcg
    11281 ggcatactga aaatgttgcc tgtggccagc ggtatttttc caccagccgt cgttggtgat
    11341 aatgaaaatg aggttggctc cgttgcgaat gtatcctgta ataaaatcac cgtagatgga
    11401 ttcgtaacaa attaccggag ctactttgca cgggctgtct ttggcgccaa aagccgtgcg
    11461 ttcttcctgt gtgccgttgc ttccggtcat gccgccgagg tcgatggcga atttttcaag
    11521 gggtttcaat agtttcggga aaggcatcat ttccaccccg ggcaccagct ttgatttgtg
    11581 ataaagctgc ggaacaaaag cagagtctaa atatagcgca gtattgcata catcataata
    11641 atactttccg cgttctgaat aacgggctgt acgcgaaagc ttttcgcctt ctttaaaaat
    11701 cttgaatgaa gatatgccga tgacaatttt tagtttggga tgtttgctta tcaggctttt
    11761 caggctgtca atgctggctg actcatttat ttcgtcctca ttgatgtatt cgggcaacgc
    11821 cgtttcgggg caaacaagaa aatctgtatt gtcatcaact ttttgttttg ccaggccgag
    11881 aatgctattc agttgttctt caatggttat ggaggtgaat ttcccataag gatccacatt
    11941 gggttgcacc accacacagt tgtacggtgc ttctttttcg gaataggaat aatacatggt
    12001 taacgatagg gctatcggga tggccattag caataccgag gatgtgccaa acagatatac
    12061 gtcttttcgg gatttgaagc ggccaaagag gcttgtcagc aatttgtaga agagaatatt
    12121 aaaaagcaaa atccagatat ctccgccaaa aacaccggta aattcatacc attggacaaa
    12181 tctgggatag ttggcaaatc cgttgcccag gttgagccac gaccaggaaa gatcccaatc
    12241 aagatgcaaa aattcaaagc ttatccataa caaaatcagc acataataac cgccacgcga
    12301 tttaaaaact gtgcgcctga tgaggtgata tgccaaaaaa gtgatcgcca taaacagcga
    12361 attgagcgct acggccatgg ctaccccaaa agtggtggaa taataaatcc accaggtggt
    12421 caggccgttc cagataaaaa atgcgatata tgcgtaaaaa aatacgctgg atttaatgaa
    12481 attatgcctg tttttggcaa tataatcttc tatgtacagc agcggtacaa aggcgaagaa
    12541 aagcaaaaag ggaaaacccc tggccggcca ggctaaagaa agtaataacc cgctaagtat
    12601 tgacagtaaa accaggtgtc cttttttcat cgtttcagca aaacaagtag caaaaataca
    12661 aattattcgg taacatcaac gggccgggtt tttatatagc ttcagataaa cctgtccccg
    12721 ggagaagtgt ttcagcggta tccattgttg ggctttggca tgaatcgctt cggggttttt
    12781 gatgttatag acattggatg tgataacata acgacaggtg tccatattgg catggctggc
    12841 atcagcatag gcaaaatctt cgtccagcca ggataaagcc tggttattgg ttactgggta
    12901 aaacgaataa atatccccag gacgaatacc ctgatgtttt acataagccg ccaggtcttt
    12961 ctcaagcgaa aaatagggta acacttttaa tgaagtttcc caggcattgc catatttttg
    13021 gggataaaaa ataaagttcc ctgcgaaaaa tcccgcgaga ataaggagga aaatgccaag
    13081 ccttattttt ttcagatgga tttgttgaag gaaataaact gtcaggatta tgagaaatac
    13141 aaagctttgc aaaaaatact tatgcccgac ggggttggag agaaaaatca ttatcaggct
    13201 gttgacagcc aggggcgcaa acaggagcca taaaagcagt ttgagtttgt gtgtgcgcaa
    13261 ttttcggtaa tacaccaaaa tacaaagtat gcaaaacagc cagggaagta tttttcctga
    13321 gtcggtaatt ttccaaagta tatagataaa ttgcctgatg accatagcgg ggccattcat
    13381 gctgatatga acaacattgg cgggattgat gattatccat ccggttacca ggtagtgaat
    13441 tatgctccac gaaatcaaaa aaaacatggc cggcagataa accagcacgt ctttgatctg
    13501 cggcctggtt ttatcataga gcaacaaaac cataaagtga ataagggcca gtgcggggat
    13561 aaaaaacaat gcccggaaag aggttgccgc caataatagc agactcagag agtagagcag
    13621 gtgttttttc tccagaaggc ttcttatggc cagcaaaaca aaatacagca ggaataaatc
    13681 ataacccatc aatattatct gtgtgatgaa tgccgggtca aacagcagca acaaataaac
    13741 cattggcaat aactccggtt tgataaaccg tagcgagatt ttttttattt ccagaaaaat
    13801 accaaccaca acggggatta tagccagatg gcataccagc agtgtctttc cgaagatggc
    13861 acaacaggct gccaggtaga gcgcgtttat ggtcagcggc ccgaaatcgg tttccaatgg
    13921 tggaataata tgccggaacg aatcgcgtaa gtaaaaattt atgtattcac taatggtaat
    13981 gttgtcccag aaaaaaggga ggttagctga gagcaggata atgattaccg caagggcaga
    14041 aaaaaataca aacgtttttc ccgggtcctg aaagaaatga gttttaggag gattaatcat
    14101 tagagctctt aatgattttt ttgatcaggt attgcggttt gttgttcagc gtaaggtata
    14161 tcctgccgat gtattcgcct atcagcccga gcaacatggt ggtcatgcct ccgaagaaaa
    14221 ttaccagaac gacaatggtg gtccagccct cgacaaaatc ttttaaaaca taaaattcat
    14281 acaaatagta aatgatcagg ccaaggccaa acaaaacgat tgccaacccc accaacgtca
    14341 tgatgcggag cggaacaacc gaaaatccgg agaataagcg aaacagcacc gaaaacgacc
    14401 tgaacaaatt gtagtttcct ttgccggtta agcgtttgta atgggttact tttatctggc
    14461 agatattgtg ggttatggtg agcaggatgc cgtcgatgta gggatatggc cccgaaaacc
    14521 ggttgatttc gttaacgatg gaatgcttga taattttgaa tggcgacaga tagatgcctt
    14581 tgggtttgtt aagtatcaga cgggctatcc gtccattgat tttgctgccg atttttttca
    14641 ggaaattgtg ttttttcttt tcatacagag cataacaaac atcatatcct tccttacatt
    14701 tttcgtacag cgccggaatg tcttcggggg catgctgcaa atcgtcatcc ataatcacgg
    14761 cataattgcc tttgatatgg cgcaacccgg caaggatggc gttgtcctgg cccgagtttt
    14821 ttaccaggtc gatggccgta taattggtgt tggcggcggt cagtgacttc aaaacctccc
    14881 aactgtcatc atggctgcag tcgttaacaa atatcacctc gtgaggaagg ttgctgaggg
    14941 cagcgctgat gcgccgggat aattccggca aaatactgct gctgttatat acagggatta
    15001 caatgcttat ttcgggagct ggcatttgtc aagaactata ataatatctt ttttaaaatg
    15061 ttcggtttta taggtggtaa aattaaactt tttacctgac acttcgattg ttttcagcag
    15121 gtcgttttct atataccgaa attcttccgg gccgttaccc gcatcggaaa cttttgctgc
    15181 caatcgcccc cacaaatttt ttttggctga aatatttttt accggttcgg aaatgatgat
    15241 cctgttagct gttgtaaata aagcattcag gattttttca ggttgttcat gaaaatgata
    15301 taacgatccg gtaattatgt ttgtatcggc agggggaagc gttatttcct gatttttcag
    15361 gtcgatttgc tgtgcacgaa aattgtttct gatggctctt ctgacaaacg tcttgtttac
    15421 ttcaaagcct gtccagcaga tattgttttt tttgcaaaaa gaggcgatga aaagatctcc
    15481 gaaacataat tcatttaccg atttccctgt gatgtatgac gttaccttcc gaaacctttc
    15541 tttgtaagaa aagctatata ataagttcat agcaaggcgg tacagcaaaa tgttacggta
    15601 gataattccg ggaggcatga tgtgcgtgtt agtttccaat caggtacatc ttgccgaatt
    15661 cttttttgaa atactggtcg gcatcggttt ttgggagttc gagggtttcg tcgttcaggc
    15721 gagttaccac gcggtacagg tacacgccat tggccagccg gtcgccaaat tcatcggttc
    15781 cgttccaggc atattcggta atgttgcggc caatgtgtat ggggccaagt tcggcaaggt
    15841 caatttcgcg gactaccttt cccgagatat tcataatctg aatcttgaaa taggtaggca
    15901 cttctgatcc ggtaagggta aaaacaaagt gggtggcatt gttgaacggg ttgggccagt
    15961 tcataacatg cgttatcgaa ggtttgttta ccacttcaaa ggagatggcg tattcgttgc
    16021 tgccgctttc gttgtgcgat atatcacggg cttctacaaa aaactgatac acaccgtcct
    16081 gtaaaaaatc agggttgtaa attatgcggc aactgttgtt gggcagcgaa gccggaataa
    16141 attgcaggat ttcctggcct tgtttcataa aatacactct ttctcgggtg ttgtttcctg
    16201 gcacttgcag atataccctg aagcaggcgg tgtcgttgag tgccagaaat ttgttttcat
    16261 ctttcaacga aatttgaatt tcgggtttag ccgaaacgat atcgccgtca aaaatatgga
    16321 taccgtcaaa agttacgtca agcataggat tgatgcggtc gcggtcaacg agaaacttaa
    16381 tttccccgat attgttgaaa tgtgtctgtt ctatctggtc gaagtcgggg ttaacctcta
    16441 cccataagct gttgagcccg ctgagtccgt tggtggaaaa cgttatcgtg tcggtcagga
    16501 tatctcctga aggatgcgga cggtgcctga aggaacccaa cggatgggta atcatgttgt
    16561 ggtccaccac ccagtatttg atcagcaggc tgtccatgtc atagtcgctg atgttctgcg
    16621 tagccgtgga aaacaggata ttttctcctt cggcaatggt gtcattaaaa aaagcaaaat
    16681 gtagcgaagg gttaagagcc gtttcgggaa cgccttcgta caacacctgc cagcgtctca
    16741 tctgggcggg ggtgtgcagc gagtcgtcgc gcatggatac cagcagtttc aggtaaggat
    16801 ataccgaagc gttgatgcgg gtgttcagat tatagatatc ggctgagtcg ggaggaaggt
    16861 tttgtatcag cgtatcggtt tgtccattga atttaatgcc taaaacaaag agtttggcgc
    16921 tgtcggtgtt ggttggttca aaaggctttt tgcgccagtg cagcgatccc cagctggagg
    16981 cgggtccgat aagttcggat tcgatatatc cttcatacca tttggtggta acgctatcgt
    17041 agagctttac gatggaggtt atggctgaac cctgcagctc gtgggcgcta ccaatggccc
    17101 cgcctttttt tccgaaaata atatacggga tggtgttggg caaagtacga atttgcgaac
    17161 tgccaaacga ctcgaaactc tggtactggt cttcgctgta atcttcggcg tagtggttgc
    17221 ggtggctgta tgcaagtatg taattgccat cagggacatt gttgataaaa ctcctcatgg
    17281 cttcgcgttc tgcaaaggtg ttgcatggaa attcccatcc ggcttctgtt tcgggttgtg
    17341 gcattgcgta attcatccag ggttcgcccg acacgctgtc gataacggcc agaatcatac
    17401 cattgccgcc cgggcccata tatgcccact ggaatttcat cacattgttt accttgtaat
    17461 attcatcctg ccagggaata taagggtaga agccggtctg gcacgtcagt gatttcacgt
    17521 cgttgacaaa atcaaaacgg cgttgcggct tgttatattt tacataccgg tacctgtcgt
    17581 ttttaaactg gtgaaaatgc gcttgtcccc agcctctttt gccgttaata tactgaaacg
    17641 aactttcgcg ccagttatag gaatgttggt cgctagagtc gatactgaca cgccagaaat
    17701 atacaatgct gtcggtagta atagggaaat tgggtgtcca cggaaccacg cctccggaat
    17761 ggttgatgtg gaagacctgt tttatcgggc tattgaaagc atcggaggtg tcgagctgaa
    17821 attcataatc ctgagagccg atgaacgcat cgctggtgga ggcctttaat gttagcggca
    17881 gtgccggcat cacagcatat ttgtaaggat atacaggtac gatatccgat gattttatca
    17941 tcaagggaac ttcggtaata ttattaattt ctgaaatttc atcgatggtc tggtagcagt
    18001 cgagatagat tctgaatttg ttcaggccaa gaccgttaat gatgtcaacg ggcacccgca
    18061 gggtgagggt gtctttaaac agcggtgctt tgattttgtt gaagtagttg gtgggcgtga
    18121 tgccgccggg aaaggtgcgt gttgtttgta cgatgatgga gtcgttgata gctcttccaa
    18181 tgttggtcga aacgatatgg atattgaacg aatcgaggtc ggaggttacc tgcgggggag
    18241 taaaaaacac attaggcggg gtgatggcgt aatcgggttt ggcaaaagag tttaaaatga
    18301 tggcagggtc gccgtgcagc accatggtaa gggtgttttc tttgataatc aggtcggggc
    18361 cctggatgga atcaatagtg tttttaataa tctctccaat gggcttccca tagtttttat
    18421 aactaatctg gtggtataat tcatctgaaa agacattcag tgcggcaggt attcccagag
    18481 aaatggacgc caaaaaacct atggagcctt tatcttcaat cagtacaaat tcttcgctgg
    18541 tgctgttttg gtccagatag atatcgccgg cccagcacga attggcaaga ataaagggat
    18601 attttccgta gttctgatat tctgagggaa catctacaga tacatcaaaa cctacggagg
    18661 cggcatgtcc gaaaaatgtc atcagggtaa ccccgttgtt aatgaggttg cgtagcgagt
    18721 ccgattggtt tatctgcatc ggttccgagg atgttttcag gaaagtgcgt acatggccgc
    18781 cgaacaaagg gtcttcgata gtatgtttgt agtgattcag gtaggcggcc agcatatttt
    18841 gttcgctcag gttgccgccc ccgccaaaat gcaggatggt tttcatccat tcctgaggtg
    18901 tttgttgcgc aagttcaaat tgctggacct tgtcgagata gatggctaca tcttcggggg
    18961 tacgtgcact gaggcgtccg gtaggtatgg ccggcataaa gagactgtct ataatgccct
    19021 gagaaaaaag caggtcggaa gggggatagc caaaagaggg caccagcgtc aattggttgt
    19081 ggtatgcatc ggtgcggtat tgcggcaggg aaccatcgga agcggcgcgg tatcctttgc
    19141 caacaaggaa taatccacgg ggtttttcgg caaaatgata aaaagcaaag cgggcaaaat
    19201 ttcttatcgc aaaagccgat ttcaaaacac cataaccaaa ctggtcgtac aggtgttcaa
    19261 cattcactgt tagcgcgtga tagcctgtgg tgttgcggta actttcataa ttttcggctt
    19321 ctgcccataa agaagggtgc gtcaggataa gataatcggt aaactctgtg ttggcaatag
    19381 tctggtaatt aatgaatttg gcatcgtaac ttaccgggta tatcttgttt attggtttta
    19441 tgttgttgtc ggatgtgagg tggcagaatt tttcgcctcc cgagttagac agcaggcatt
    19501 tgaatgcagt gccatcgtac accactttta tgcgccggtg gttggtgata tcgtacagcc
    19561 tggctgtgtc gcccgatggg atattgaaat tggtgaattg cagtaatgat tttgcctgaa
    19621 gggcatccat agccgaaaaa tcaaatacgg aaacgccgcc caggttgagg gtgtgaggat
    19681 atttaatctg tatataggca atagtgtttc tgtcggagcc cacgcccagg tcgttcaccg
    19741 agctgaatac aaacggggtg gaggtgttgc ctatttgcgc aacagggatg gtgtaattat
    19801 accggagcac ctggtatccg tcaaatagcg tgtcaatggt aatccccgca aagtttatat
    19861 tcaggtgatg atctatgtaa actccggcat agtttgattg cccgaggacc acaaaatcca
    19921 cttccgcgtc agggccgttt gcgtatttgt tagcggatga gatgtttttt gtaacagact
    19981 gcccgacgtt gaatccgttg tcgaaccatc cctcattggc ggtatattca ggatcggtga
    20041 tgcctcccga gtttgtttct cccgcgtaat aggcggaggt atagttttgg cggacagtgt
    20101 tgaagatgta aggcacgggc gagtagctgc tgaaattcac atcgttgtcg gactgcaacc
    20161 tgcggttgct gaacgagttg ttccaggtga taaaatatac tgaggagtcg ctgaacaggc
    20221 tgtaggcctt gttggcctgg ccttccgggt ttttgtagag cggggtgtct aaagttccgt
    20281 cgtttttttc tgcgtaaaac tctatatagt cgccggggtt aaaaatgttg tcgttttcac
    20341 ccttgatgta gatatattgt tctatgcctt tgttgaagac ctgtatattg cgggggttaa
    20401 cgctgcccag ggggaagccg ttgctcagca gggtgttgta atcaatgcgg taaacaccgt
    20461 tttcgtgaat atagacttta taataagatt gtgaatagtt gatccattca ttgccgtatg
    20521 gttgcgcaaa ggccggcagg cataaagaga aaagaaataa aacagataaa agtcttttca
    20581 taggccttag aaaaagctgt ttttttgttt ttcctgattt ttttcgacag cggttttttt
    20641 agggttgatg ttgagtttaa ctgaaaaaac atgtgaatac aacgctatgg aagcattgcc
    20701 aacatcagta aaggcatagt cgatggctac aattttttta atcacaatac ctacgccgat
    20761 gttgggctga aaggatattt ttttcttgcc gtcgatattg gtttcttttt gtatgtttcc
    20821 gaagcctccc cgcaaaaaga ccatgtcgtt atagccaaat tcgagtccgc cgtgggggtc
    20881 aatggaaatg gggttgcttc tgataaggac gttgcgtttt ccgtcgaagg tcatgtcgag
    20941 gtcgatggcc accagggcag aaaacttttt gggaaaatca aagcggcggg cggcacccag
    21001 gttgaggcgg ggcagggtaa gctccaggga gttttcgggt atttcgttgc ctgtccgggt
    21061 aaacacgtct ttcatgttgt cgtcgagatt gtatttccag gcgttgaaag tagaggtgat
    21121 gtctcttgct gtagctccga aatgccattt tttcaggtcg tactgtactc cgatatccaa
    21181 tccgaaaccc caggaatgag caaaagaacc caccttgcgg taaattatct tggcgctgcc
    21241 gccgtagcgg aggccttcaa tttttgattt tcgggcatag gacaggagta tggcataatc
    21301 ggcagccgaa aatgtggtga cgcggtcgta atcaatgttc ccctgggcat caatcagttc
    21361 ggtggtgttg gggatatcgt caacgccgaa gcgtatgaaa gtgagtgccg ccgtgctttt
    21421 atcgtcaatc tgtacggcca gcgcgccata gtcgtatttt gctatgccgg cgaaatattc
    21481 tgaatgcatc agcccgacct ggaatttgga aggtaccatg gccaggccgg cagggttcca
    21541 gtatcccgag gttacatcat ttacaaacga gacattggag tttgacatcc ccagggcacg
    21601 ggcgcctacg ccaatagaca aaaattcatt gctatacttg ggagcctgcg catggaccat
    21661 gcccatgcca atgaagcata ctatagtgat ggtaaaaaac ttacgaatca ttctctgttg
    21721 ctgtttttta atggtgcaat agtaacggga aaacacttaa ataattataa aattataaaa
    21781 aatgtttcat aaaggtataa aaaaaaatat tatcaggcgg ttaacaaaaa aataatcagc
    21841 ctatttcaac aggcaggaaa gacgttttag ctctatttca gctactttgg cctgatattg
    21901 caagtttacc aggaggtaag ccgagttttc ataactctgc tgggcggatt ttaaatccag
    21961 gatggtagcc tggttcaact gaaatttctg aagtaccagc gccaccagtt tttcagaaag
    22021 ttgaaatgag gcctgttgta tatctagttg gtctaaagca tttttgtatg cctgatgggt
    22081 ttttcttgct gatgaggtca gttcgttaaa taaattttct ttttccagcc tggcagtgct
    22141 ggtattaagc atagcggttt tttgacgtac gcggtaggtg ccgccgttaa atatcggtat
    22201 ttgcaggttg aggccggcat aggggccata attaaggctt gtcagggtgt tccctttagt
    22261 gtttttagca aaccctacct cataggcggt atttagccga agcgatggca tacgttctgc
    22321 gcgggcttct ttggcaagct gttcactgat ctttatctgc tgttcggcgg taaggtattg
    22381 gtagttatct tttaaaaaca gcaacaccga gtcgagcgta atttgcggat ccacagttat
    22441 caggctgtct ccggtaataa actgttgttc ctggctgcca cccattaact gcaacaactc
    22501 tgtttttgag ttttcaataa cgagttgttg tgagcggaaa ttttgttcgg caaggttgag
    22561 gtcgatttgt gcctgcatca tgtcg
//
LOCUS       DAOSFP010000003        20117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_2168_length_20117_cov_4.407935, whole genome shotgun sequence.
ACCESSION   DAOSFP010000003 DAOSFP010000000
VERSION     DAOSFP010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20117
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_20117_cov_4.407935"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="PLI16_00230"
     CDS             complement(<1..678)
                     /locus_tag="PLI16_00230"
                     /inference="COORDINATES: protein motif:HMM:NF007757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOH83014.1"
                     /translation="MQDLNITIVQSDLAWEQVQKNLDTFDKKLEGLKNEQDVVVLPEM
                     FATAFTMNVGDFGGNMDSPVITWMKEKAEALNSAITGSFIYKEKDVFYNRLVWMNPDH
                     NFETYDKRHLFRFGGENNFFKAGKQKIVVEHKGWRVFPLICYDLRFPVWSKNKLVNEN
                     YQYDVLIYVANWPETRKQHWKTLLQARAIENLCYVVGVNRIGCDGRGTRHSGNSLIIS
                     PRGEILHE"
     gene            795..2009
                     /locus_tag="PLI16_00235"
     CDS             795..2009
                     /locus_tag="PLI16_00235"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH83015.1"
                     /translation="MVNSHLRLKTIDKVNFKNKKVLIRVDFNVPLDQNRQITDDTRIR
                     ESLPTIQKVLNDGGTVILMSHLGRPKGGFEQDYSLTPVVPRLSKLLNRNIIFTRDLFG
                     PKTFEVCGALKPGDVVLLENLRFYPEEEKGDKNFAKELASLGDIYINDAFATAHRKHA
                     STATIAEFFPNNKYFGLLLANELKNLNFILNEAKSPFTAIIGGAKVSSKISIIKNLIN
                     KVDNLIIGGGMAFTFIKAFGGEVGGSLVEDEKIPEVMEMVQEMMLRGVNLYIPTDAVI
                     ADGFYDEANFKESQADAIPKNWMGLDIGRKTCRRFADIINASETIIWNGPMGVFELPH
                     FKQGTKSIAIAVASATIRGSFSLIGGGDSVAAINQYNLADQISYVSTGGGAMLEFIEG
                     KELPGVKAIMED"
     gene            complement(2015..2554)
                     /locus_tag="PLI16_00240"
     CDS             complement(2015..2554)
                     /locus_tag="PLI16_00240"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOH83016.1"
                     /translation="MNYLDIIILIPLGFAVFKGLRKGLIYELAVLVSLFVGVFAGIYF
                     SDFIAGLLIHNLGMNEMYTKAVAFTLIFIAVIVLVRMFAKAIESLIDMTALSLANKIL
                     GAVFSVLKIALVLSVIFFIINRYDPREALITHQAKEKSFMYKPIYSIAPIAIPKINSE
                     IKKWQAQKDSIPQDKNQAE"
     gene            complement(2556..3302)
                     /locus_tag="PLI16_00245"
     CDS             complement(2556..3302)
                     /locus_tag="PLI16_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH83017.1"
                     /translation="MIETRNISKGFADKKVIKDISVIFERGKTNLIIGPSGSGKTVLM
                     KCIVGLLDVDEGHILFDDRPFTEMSFNEKKVIRQEIGMMFQGGALFDSMNVEENVMFP
                     LTLFTKKSLEEKRDRVNFCLKRVNLENVNKLYPAEISGGMKKRVAIARAIAMNPSYLF
                     CDEPNSGLDPKTSILIDNLISEITQEYQMTTVINTHDMNSVIEIGDKIAFVYTGKLWW
                     VGDKHQILDTDNAELNNFVYATELTRRLKK"
     gene            complement(3305..4003)
                     /locus_tag="PLI16_00250"
     CDS             complement(3305..4003)
                     /locus_tag="PLI16_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH83018.1"
                     /translation="MKKVFSKPEKGPVFRQQLFTEMDNLGVDSLGIVAIISVFMGAVV
                     AMQTGYNTTSAWIPEYTVGFTTRESIILEFSNTIICLILAGKVGSRIASEIGTMRVTE
                     QIDALEVMGINSASFLILPKIVAAMLIFPVLVIISMFLGILGGWFAAAWMNVVTVSAY
                     YDGITLDLIPFEIFYSIVKTIAFSLIITSVSGFYGFNAYGGALEVGRASTKAVVMSSI
                     LIILFNLVITQLML"
     gene            4383..4526
                     /locus_tag="PLI16_00255"
     CDS             4383..4526
                     /locus_tag="PLI16_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83019.1"
                     /translation="MKANKILYLLVFVVLIIISGCASKKHRYNQCPTFSKAQPTEKVQ
                     NIG"
     gene            4519..5151
                     /locus_tag="PLI16_00260"
     CDS             4519..5151
                     /locus_tag="PLI16_00260"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOH83020.1"
                     /translation="MDKESLAPYFPETSLDTVFHWMQSHPLQLIITRERATKSGDYRP
                     PDKEKPYHRISVNHNLNKYAFLITFTHEYAHLITWNAFKRKARPHGDEWKANYRSLVF
                     FLLEKNIFPPDLSHALVKIFVKNGEPVHAAEYNLTLTLAKYDTNATIEFLEELAPDSV
                     FSIHNGLVFRKGEKLRTRYKCFCLNNKRWYLINAAMKVNPVSKAFDFELN"
     gene            5236..6330
                     /locus_tag="PLI16_00265"
     CDS             5236..6330
                     /locus_tag="PLI16_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOH83021.1"
                     /translation="MSNNYCVIMAGGIGSRFWPMSRTSHPKQFIDILGTGETLIQQTF
                     RRFEKIIPKENIFIVTNEKYADLVREQIPSLETRQIISEPARRNTAPCIAYACYKINE
                     TDPDANVVIAPSDHIILNEDSFSGIIISALQAASKNNWLITLGINPSRPDTGYGYIQY
                     KDTDGYPEDTRLKKVKTFTEKPVLEMARQFIESGDFLWNSGIFVWSIKTILKAFETHL
                     PEIYESFEKGKGIYNTEKEQEFIQHTYTTGKSISIDYGVMEKADNVYVLCSDFGWSDL
                     GTWGSLYENRKKNEAGNAIVGKDVIMYDSHNCIVNMPEDKLVVLQGLDDYIVVESGNV
                     LLICRKNDEQQIRNFVNDVKIKIEKGEQYI"
     gene            complement(6383..7771)
                     /gene="gatD"
                     /locus_tag="PLI16_00270"
     CDS             complement(6383..7771)
                     /gene="gatD"
                     /locus_tag="PLI16_00270"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOH83022.1"
                     /translation="MSEDFFQGYKGDALEILKKYNVRVWGQAEVDTTRGLFKGTVLPR
                     AENDDDKHIVLKVVTGYNIGIDISTILTMKEVGYKKANYKIPEKEFPYTPGKPKVKLI
                     GTGGTIASRLDYRTGAVIPAFSPGELYGAVPELADICNLETEKLFAVFSENMGPEQYK
                     KLAVAIGQEIEKGTDGIIIGHGTDTLHYTAAALTFMVQNPPIPIVLVGSQRSSDRPSS
                     DAALNLMHAATAAAHGDIAEVMVCMFGPTSDEYGFLHRGTRVRKMHSSYRSTFRTIGD
                     IPVATVTRNGVVPQKPVYNHRRKDTSNVKITPVFEEKVGMLYYYTNMQPDMIDSMVDN
                     GYKGIVIIGTGLGHVNKPIYPAIERAVKKGVAIYMTVQTLWGYVHMFVYDTGRDLMAK
                     GIIPAENMLPEVAYIKLGWALGQTNDLEKVKEIMLTPINDDITPREPYNGYLIYQGGV
                     KEVEDFIKKVHK"
     gene            complement(7792..9765)
                     /gene="gatE"
                     /locus_tag="PLI16_00275"
     CDS             complement(7792..9765)
                     /gene="gatE"
                     /locus_tag="PLI16_00275"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOH83023.1"
                     /translation="MKENFDPKLNYEETKKAIGYTPLKQSTQEDWNRIGFMSGLEVHQ
                     QLKTKEKLFCHCPAGVYQKKNQYDAELLRHMRPTLSELGEYDGTALMEFKTRKEIVYR
                     VKNETTCTYDVDDTPPFKLNREALENALEIALLSKLNIVGEVHITRKQYLDGSIPTGF
                     QRTAILGVEGELPLKTKNIRLIQLSIEEDSCREISDIGHRRIYSTDRLGVPLIETVTY
                     PDMVNPYEVKEACDYIRFLNRSTGKVRTGIGAGRQDVNVSCKGGTRIEIKGVAHTRWI
                     PKLTHNECFRQWALLHIREILNGKFGDGSSWKMNYQEVQLKDFHFSNLPLNEEKKKAY
                     KYYAINLPGFKKILSHFTQPGKMFADEISERLKVIACIELPNMTHSEEFEPLISGDDF
                     SNIRASLNAKEDDAQIILWGPADDMKTALETIEERCQWAFKGVPNETRKSFENGTTIF
                     ERVLPGADRMYPDTDSVPIPLEDEYINRLKKNLPTEVIERYQKLKEWGIPEDTFTYIF
                     KKNLFPVIEKIISVNGYPPKFIGTFIGHTLKYFEGQHAISPYFNYEMIGDMFAFISEK
                     KLDKQIAKKMLPILIQHPKMDFESILATIKFKKITKEEILSNIPFMKEKFHQIRISQQ
                     PDVETKWIMGGLRKMATGNICLAELKGLVETSN"
     gene            complement(9952..11994)
                     /locus_tag="PLI16_00280"
     CDS             complement(9952..11994)
                     /locus_tag="PLI16_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83024.1"
                     /translation="MKKTRLYLIALAILFAAGLVITYSNHFNNGFHFDDSHTIQDNPY
                     ITSLKNVPLFFKDTKTFSSMPSHQGYRPMVTTTLAVDYWLGGGLKPFYFHLSTFLWFV
                     LLCILLFFVYKKISKTALKHTWAGYVALVAAAVFAFHTVNAETINYIISRSDVLSTLC
                     IIASVAIFILFPHLRKWGIYILPAILCVGFKETALIIPVLLFFYVIFFEKNLSLTDLF
                     KACNLKTLGKTVLMFVPILIIIGLLQYYSYAKMPREGIENIDNPVLPYVLTQTFVWVH
                     YFLSFFLPINLSADTDWTVLSNPFDDRIMVGVIFVIVLVFVIFKTASKAEQRPIAFGL
                     IWFTVSLLPTSLVPLTEVLNDHRMFLPFTGLTFSVIYTVGLLVVKNEHIFIKNRKNLV
                     VLWVAVFLILSAFAYGTHQRNEVWKDEESLWLDVTIKSPKNGRGLMNYGLTQMAKANY
                     PVAEKYFEQALEYTPYYSFLHINLGILKNAMGKPAEAEGYFNKAIQYGPQYNEPYYYY
                     AGFLLKQNRIQEARRMEEQDLLINPIFMQGLYQMMDIYNRLELWEKLDSVALHTLSIS
                     GGDTIALKYREAAVKKVSAADQHLQFARQNPTPENYLNLSLQYYHLGQYEKCIEACNE
                     ALKLKPDYAEAYNNICSAYNAMHMWDKGAEACEKALKINPSYELAKNNLAWAKSNL"
     gene            12166..12756
                     /locus_tag="PLI16_00285"
     CDS             12166..12756
                     /locus_tag="PLI16_00285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH83025.1"
                     /translation="MKTQDKEYAGRLYKNQYVWWKRLLDVQRPYRWNLRRLKPGFVLD
                     IGCGIGRNLIHLDGNGIGIDHNLAAVEIAKKKGLQVFTPDEFHTSEYNIPLRFDSLLL
                     AHVAEHMTEKEAIKLLEIYFPLLKNNGNLIIITPQETGYKSDATHVQFVNFDVQRRII
                     KALNLEFVRQYSFPFPRFFGSFFKYNEFISIGRKQA"
     gene            complement(12779..14209)
                     /locus_tag="PLI16_00290"
     CDS             complement(12779..14209)
                     /locus_tag="PLI16_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH83026.1"
                     /translation="MILVVVPTMENAYHNLKHFVSVSPPIGLTSIAATALRAGCDVSI
                     IDGDAENLTMEKLVNMVAEKKPAFVGATIMTATMDITREFLTLLKKKTPETVMIAGGP
                     HVSALPKQTLEDTWALDIGVIGEGDLTIIDIVNAYKNQLSLQDVDGIVYRKNDRIVIT
                     GYRPPIANLGCLPVPANHLLKKHLYRSYLWSRWVSGQNGPIGVIFTARGCIGRCNFCA
                     SHTVFGPKVRYFDMQQVYDQLDFFIKEWNIRILYLQDDTFNLNRKRVIELCDYIIEKG
                     YNKRLEIQISSRANTAHLPTLKKMREAGVRWVFFGIESGNQQILDQMDKNIRLDQIRS
                     AVANARKAGMFVGGNYIIGCLGETYETASDTIKLACELQQDYASFATAIPLPGTGLYQ
                     HCLENNISLPPWNDFGSVNTPPIALNNTLTAEQLSQLRDMAVNKFFIRPGYFIKILIR
                     LKTFIVLKDFVTMFFALRKEKKEKRL"
     gene            complement(14232..15011)
                     /locus_tag="PLI16_00295"
     CDS             complement(14232..15011)
                     /locus_tag="PLI16_00295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83027.1"
                     /translation="MEDHTFVIPAYKDSVFIEECIVSLKNQTKKSHICITTSTPSQHL
                     KDVAQRHSLPLLVNNNSKDIAGDWNFALSEAQTTWVTLAHQDDIYESNYLENVLEKNN
                     NKVLILFTDYCEIIHGKIRRHSLNFFTKKILLIPFLIKPCIKNRFFKRLPLIFGNAIC
                     CPSVTFNKNHLKDFAFSPDFSYSLDWKAWLDMANRDGAFVFINKKLMKHRIHEDSETT
                     RQLVTNVRKKEELRIFGIIWGKRIAGMLGRIYSLSHKDNIL"
     gene            complement(15017..16498)
                     /locus_tag="PLI16_00300"
     CDS             complement(15017..16498)
                     /locus_tag="PLI16_00300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH83028.1"
                     /translation="MKILLVNPPFYRIIGFYNRYFPLALTMLATTLKCAGHEALVYDA
                     DLYNTPENMDYSLLPEKYPVFLSSLQDADHPVWAEVKKTISDFCPDLIGISIYTTFAA
                     SAFHTAEIAKTIYPDCPVVLGGPHATSKAGEVMQIAPFVDFVVKGAGEKPLLDLANHF
                     QRKEGSLQSIAGLSFRMDGQIIHNPAADTRERISGTSFPDRSLLMNEKSYSSEDMGLI
                     MTTIGCPYNCTFCASHIKKISSREVDNIIEEIKLVKQKYGTTQFAFKDDSFTINKSRV
                     YELCQRLIGEKLHICWECNTRVNLIDEELLRVMKKAGCNYIKIGIESGADKILAKINK
                     GITCEQSRNAAKILRKSGIHWSAYFLIGMLGETKEDMYNTLEFMYELKPDLALLGVYE
                     TFPGTKMFDDGLEKNMVKTPMLLEDFYSTLPHLYYIKNPHVRSDQLDEATFIQVVSDI
                     KEKFHRYNKKPRHVIKTALAKTSVYRREPKILLADVRKFFRYI"
     gene            complement(16498..17379)
                     /locus_tag="PLI16_00305"
     CDS             complement(16498..17379)
                     /locus_tag="PLI16_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH83029.1"
                     /translation="MEKQLTIIIPAYNEESSLRSFLPEVIAFCRQQGFQLIVVDDGSV
                     DKTYETLKDLTFGNDFVEIIHHKVNRGYGGAIKTGIQNTTTPYLITIDADGQHYLEDV
                     LKLLDKTKETEADMVIGSRKGQKIHNYYRHFGKSIIRSFAKMTMTVPIYDINSGMKLC
                     DTALAQKYIPLCPDDMAYSDFIALIFINQKHLVIEEPIRIRERREGKSKISLGTAYST
                     ILAIIHILLLFNPMRFFLPIALLCIIFGIAWGLPFLLMGRGVSNGAIISILSGLILVF
                     LGLIADQISMFRKERLK"
     gene            17814..20009
                     /gene="recQ"
                     /locus_tag="PLI16_00310"
     CDS             17814..20009
                     /gene="recQ"
                     /locus_tag="PLI16_00310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOH83030.1"
                     /translation="MKEKSGDKLHIVLKEFFGFDTFKGNQEAVIKNVLDGKDTFVIMP
                     TGGGKSLTYQLPALISDGTAIIISPLISLMKNQVDNIRNFGVDKGIANFLNSSLSKQE
                     MTDVKADLLAGKTKLLYVAPETLTKKENIEFFKQFKISFYAIDEAHCISEWGHDFRPE
                     YRNIRPTIKAIGKKVPVMALTATATPKVQHDIQKNLGMMNAGLFKSSFNRPNLYYEVR
                     AKTKDVQRDIIKYIKSNSGKSGIIYCLSRKKVEELAETLQVNGIKALPYHAGLESEVR
                     KANQDKFLMEEVDVIVATIAFGMGIDKPDVRYVIHHDMPKSLEGYYQETGRSGRDDGE
                     GNCIAFYAYEDIQKLEKFTSKKSVNEQEIAKQLLMETIAYAESSVCRRKLLLHYFGEV
                     FEEENCQCCDNCLHPKTKFDGTAYVETVLEAVVETKEQCKGAHLVNVLVGKVSAQVKS
                     FKHHKLNVFGKGADHDEKFWTAVIRQTIVNGLLSKDIENYGILKITKEGEKFLDEPET
                     IMLTQDHDYDTPEEDEFFAGGGKTSTADKTLFTLLKDLRKQISRKENLPPFVIFQDPS
                     LEDMAIQYPIKMDELMQITGVGSGKAQKYGKPFLDLIAKYVEENEIIRPMDMIVKSVV
                     NKSGLKVYIIQNIDRKIPLEDIANAKNLTIEELLMELESIVASGTKLDINYYIEEYVE
                     PYHQEEIYDYFYEAETDSVEDALKELGEEEFSEEEIRLVRLKFLSEVGN"
BASE COUNT         5820 a         4306 c         4283 g         5708 t
ORIGIN      
        1 ttcatgtaat atctcgcccc tggggctgat gattaacgag ttgcccgaat gacgggtgcc
       61 gcggccatca cacccaatgc ggttcacgcc aaccacataa caaaggtttt cgatggcacg
      121 cgcctgaagc agggttttcc agtgctgctt acgggtttca ggccagttgg caacataaat
      181 tagcacatca tactgatagt tttcattcac cagtttattt ttggaccata caggaaacct
      241 taggtcataa caaatcaggg gaaaaacacg ccagccttta tgttccacga caattttttg
      301 ttttcccgct ttaaaaaaat tattttcacc accaaaacgg aacaaatgtc ttttgtcgta
      361 agtctcaaaa ttatgatcgg gattcatcca caccaggcgg ttgtaaaaaa catctttttc
      421 tttataaata aaactgccgg taatagccga gttgagcgct tctgcctttt ctttcatcca
      481 cgttataaca gggctgtcca tgttgccgcc aaaatcccct acattcatag taaaagccgt
      541 agcaaacatt tcgggaagca ccaccacatc ctgttcgttt ttcaggcctt cgagcttctt
      601 atcaaaagta tcgaggtttt tctgcacctg ttcccaggcc agatcgcttt ggacgatagt
      661 aatgtttaaa tcttgcatac tattatgatt acaacaaata tatgtttttt tcgctgttta
      721 ataaaaaaca catatttatt tttgattatc agccattgat tattattttt actgaaattt
      781 ttaaaaataa ttttatggta aactcacacc tcaggttaaa aaccatcgac aaagtaaact
      841 ttaaaaataa aaaagtactg atacgcgtag atttcaatgt tcctctggat cagaacaggc
      901 agattaccga tgacacacgt atcagggagt cgttgcccac catacagaag gtgttgaatg
      961 atggcgggac ggtaatcctg atgtcccatc ttggccgccc taagggaggt tttgaacagg
     1021 attattcgtt gacgcccgtt gttccgcgtt tatcgaaact gctcaaccgt aatattattt
     1081 ttacccgcga cctgtttggc ccgaaaactt ttgaggtgtg cggggcgctg aaacccggag
     1141 atgtcgtgtt gttggaaaac ctgagatttt atcccgaaga agaaaaaggc gacaaaaatt
     1201 ttgctaaaga gctggcctcg ctgggcgaca tatacattaa cgatgccttt gctacggcac
     1261 accgcaaaca cgcttcgacg gccaccatcg cggagttttt tcccaacaac aaatactttg
     1321 ggttgttgct tgccaacgag ctgaaaaacc tgaattttat ccttaacgaa gccaaatccc
     1381 cttttacagc tattattgga ggagcaaaag tttcttccaa aatttctatc ataaaaaatc
     1441 tgatcaataa ggttgataac ctcataattg gcggtggtat ggcctttacc tttatcaagg
     1501 cctttggcgg cgaggtgggc ggttcgctgg ttgaagacga aaaaattcct gaggtaatgg
     1561 aaatggttca ggaaatgatg ctgcggggtg tcaacctgta tatccccact gatgcggtga
     1621 tagcagatgg tttttatgat gaagccaact ttaaggaaag ccaggccgat gctatcccca
     1681 aaaactggat gggactggat atcggaagaa aaacctgccg tcgttttgcc gacatcatca
     1741 atgcctcgga aaccattatc tggaatggcc ctatgggagt ttttgagctt ccacatttca
     1801 agcagggcac caaatctatc gctattgccg tggccagcgc cactatacgt ggttcgttct
     1861 cgcttatcgg cggcggcgac tcggtagcag ccattaacca atataacctg gccgaccaga
     1921 tcagctatgt ttctaccggc ggcggcgcta tgcttgaatt tatcgagggc aaagagctgc
     1981 cgggcgttaa ggcgataatg gaagattagc agacctattc tgcctggttc ttgtcttgcg
     2041 gaatactatc tttttgtgcc tgccattttt ttatttctga atttattttt gggatggcta
     2101 taggggcaat actgtatatg ggtttgtaca taaatgattt ttctttagcc tgatgggtta
     2161 tcagggcttc gcgcgggtcg taccggttga tgataaagaa aatcacgctc agcaccaggg
     2221 caatcttcag aacagaaaac acagccccga ggattttatt tgccaggctc agggcggtca
     2281 tgtcaatcaa agattctata gctttggcaa acattcgcac aagcacaata acggcaataa
     2341 aaatcaaggt gaaggccact gccttggtgt acatctcatt cattcccagg ttgtggatca
     2401 gcaaccctgc gataaaatcg gaaaaatata tcccggcaaa aacacccaca aacaaagaca
     2461 ccagtaccgc cagctcataa atcaggcctt tgcgtaagcc cttgaaaaca gcgaatccca
     2521 gcgggatcag gataataatg tcgagataat tcatgctatt tttttaagcg ccgtgtgagt
     2581 tcggtggcat acacaaagtt attcaattcc gcgttgtcgg tatcgagaat ctggtgcttg
     2641 tcgcccaccc accagagttt tccggtgtat acaaaagcaa ttttgtcgcc tatttctatt
     2701 actgagttca tgtcgtgcgt gttaataacc gttgtcatct ggtattcctg tgtgatctcg
     2761 ctgatgaggt tgtcaatcag gatggaggtt ttcggatcca atcccgaatt gggttcgtcg
     2821 caaaacaggt agctgggatt catggctata gcccgtgcta tggcaacacg ttttttcatc
     2881 ccgccgctga tctctgccgg atacaatttg tttacatttt cgagatttac acgtttgagg
     2941 caaaaattca ccctgtcgcg tttttcttcc aatgattttt tggtaaacaa ggtcagcgga
     3001 aacatgacat tttcctccac attcatcgaa tcaaacaagg cgccgccctg aaacatcatg
     3061 ccgatttcct gcctgataac ttttttttcg ttaaaagaca tctcggtaaa aggcctgtcg
     3121 tcaaacagaa tatggccttc atcaacatcg agcaggccaa caatgcattt catcagtacc
     3181 gtttttcccg agccgctcgg gccaataatc aggttggttt ttccgcgctc aaaaatcaca
     3241 ctgatgtctt tgatcacttt tttatcggca aatcctttgg atatgttgcg ggtttcaatc
     3301 atacctataa catcagttgg gtaattacca ggttaaacaa aatgataaga atactgctca
     3361 tcaccacagc cttggtgctg gcacggccaa cttccagggc cccgccatag gcattaaaac
     3421 cataaaatcc ggatacagat gtgatgataa gagaaaaggc aatagttttt acgattgaat
     3481 aaaatatttc aaaaggaatc aaatcaaggg tgatgccgtc gtaataggcc gaaacggtta
     3541 ccacattcat ccaggcggcg gcaaaccatc cgcctaaaat tcctaaaaac atactgatga
     3601 taaccagcac gggaaaaatc aacatggcgg ccactatctt gggaaggatc aggaaactgg
     3661 ccgagttgat gcccattacc tccagggcgt caatctgctc ggtaacccgc atggtgccta
     3721 tctcggaggc aatacgcgaa cccactttgc ctgcaagaat aagacagata atggtgttgg
     3781 aaaactcaag aattatggat tccctggtgg taaacccgac ggtatattcg ggtatccagg
     3841 cgctggtggt gttataacct gtctgcatgg ccaccacagc ccccataaat acggagataa
     3901 tagccacgat gcccaatgaa tcaacaccca gattgtccat ttctgtgaaa agttgctgac
     3961 ggaaaaccgg gcctttttca ggtttcgaaa acaccttctt catcaacaac aggtagtttc
     4021 ctatagtggt taatgctttt ttcaagctgc gtgattttat tttctgcgaa tttagcaata
     4081 ttttgaaaac atgctgcaaa aattgataac tggttatcct tgaagagatg atgaaaaatt
     4141 attttatttt taataacagc atgcattttt gccggaaata acaggttttt tctttaattt
     4201 tgcatgatgc tttactacga taaaattccg gatgaaaaaa attaaaatat cactggtgtt
     4261 tttaatgctg atgtgctttg cttttgctgc ccaatcgtgc aaaacatctc aaaagcacaa
     4321 atgcaaagac tgtcctacat tttcacaaaa caaaaacggt aattttcaaa acaataattt
     4381 atatgaaagc taacaagatt ctttacctgc tggtgtttgt tgtccttatt attatttcgg
     4441 gctgtgcttc aaaaaaacac cggtataatc aatgtcccac ttttagcaaa gcccaaccca
     4501 ccgaaaaggt tcaaaacatt ggataaagaa tctctagcgc cttattttcc cgaaacaagt
     4561 ttagatacag tattccactg gatgcaatca catcctttgc agttgattat tacacgggaa
     4621 agagccacca aatcgggcga ctaccgtcca cccgacaaag aaaagcccta tcacaggatc
     4681 agcgtcaacc acaacctgaa caaatatgct tttcttataa cttttaccca cgaatatgct
     4741 catttgatta cctggaacgc ctttaagaga aaggccagac cgcatggcga tgaatggaag
     4801 gccaattaca ggtcgctggt atttttttta ttggagaaaa atatttttcc gcccgattta
     4861 tcgcacgcct tggtaaagat ttttgtaaaa aacggtgaac cggtgcatgc agccgaatat
     4921 aacctgacac tgaccctggc gaaatatgat acgaatgcga ctatagaatt tctggaagaa
     4981 ctggcccccg actcagtgtt ttccattcat aacgggcttg ttttccggaa aggcgaaaag
     5041 ctccgcaccc ggtacaagtg tttttgcctg aataacaaac gctggtatct gatcaacgca
     5101 gccatgaaag tcaacccagt gagcaaggcc tttgattttg agctgaatta agcaccgacg
     5161 atttaaaatg aaaaaacacg aggataataa atgataattt gatatttttg ccaaaaatta
     5221 aaataaaata taataatgag caataactat tgtgtgataa tggcaggcgg cataggttcg
     5281 cgtttctggc ccatgagccg cacttcccac cccaaacaat tcatcgatat tctcggcacg
     5341 ggagaaacgc tgatacagca gactttcagg cgttttgaaa agatcattcc aaaagaaaac
     5401 attttcattg taaccaacga aaaatatgcc gatctggtca gggaacaaat accctctttg
     5461 gaaacgcgtc agataatttc tgagccggcc cgcaggaata ccgcaccgtg tattgcttat
     5521 gcctgttata aaatcaatga aaccgacccc gatgccaatg tggtgatagc tccttccgat
     5581 cacattattc ttaacgaaga ttctttttca ggtatcatca tctcggccct tcaggcagca
     5641 tcaaaaaata actggctgat taccctgggc attaacccca gccgccccga tacgggttat
     5701 ggctatatcc aatacaagga tacggatggt tatcccgaag atacgcgcct gaaaaaagta
     5761 aaaacattta ccgaaaaacc cgtgctcgaa atggcccggc agtttattga aagcggtgat
     5821 tttttatgga attcgggcat tttcgtatgg tcaataaaaa ctattcttaa agcttttgaa
     5881 acgcatcttc ccgaaatcta cgaatcgttt gaaaaaggta agggcatata caatacagaa
     5941 aaagaacagg aatttattca acacacatac accaccggta aaagcatttc tattgactat
     6001 ggcgtgatgg aaaaggctga taatgtatat gtgctgtgtt ctgatttcgg ctggtccgac
     6061 ctgggcacct ggggctcctt atatgaaaac cgcaaaaaaa acgaagccgg caatgccatt
     6121 gttggcaaag atgtaattat gtatgacagc cacaactgca tagtaaatat gccggaagac
     6181 aagctggttg tgctgcaggg cctcgacgat tatattgtgg tggagtcggg aaatgtattg
     6241 ttaatctgcc gcaaaaacga cgaacagcag attcgtaatt ttgtgaatga tgtaaaaata
     6301 aagattgaaa aaggggaaca atacatttaa aagttgaagg ttaaaataaa aagaaaaggg
     6361 ctgaatttca gcccttttct ttttatttgt gtactttctt gataaaatct tcgacttctt
     6421 ttactccgcc ctggtaaatc aggtatccgt tgtaaggttc gcgcggggta atgtcgtcat
     6481 tgatcggcgt cagcattatc tcttttactt tttcgaggtc gttggtttgg ccaagcgccc
     6541 agcccaattt gatataagcc acctcgggca gcatgttttc tgccggaata atgcctttgg
     6601 ccatcaggtc gcggcctgtg tcgtaaacaa acatatgtac atagccccac agggtttgta
     6661 ctgtcatata aatggccacg ccttttttca cggcacgttc tatggccgga tatataggtt
     6721 tgttgacatg ccccagtccc gtaccaatga tgacaatgcc tttataaccg ttatcaacca
     6781 tcgagtcgat catatcgggt tgcatattgg tgtagtaata caacatgccc accttttctt
     6841 caaaaacggg tgtgattttt acattggaag tatccttgcg gcggtgatta tacaccggct
     6901 tttgcggcac cacgccatta cgtgttaccg tagccacggg aatatctccg atggtgcgga
     6961 aagtagaacg gtatgacgaa tgcattttac gaaccctggt gccacggtgc aggaacccgt
     7021 attcgtcgga tgtaggtccg aacatgcaca ccatcacctc ggcgatatcg ccatgagcgg
     7081 ctgctgtggc ggcatgcatc agattcaggg ccgcatcgga gcttggcctg tctgaggagc
     7141 gctgtgagcc taccagcacg atagggatgg gaggattctg aaccataaaa gtaagtgcag
     7201 cggccgtata atgcaaggtg tcggtaccat ggcctataat aataccatcg gtgccttttt
     7261 ctatttcctg tcctatggca acggcaagtt ttttgtattg ttcgggcccc atattttcgc
     7321 tgaacaccgc gaaaagtttt tcggtttcca ggttgcagat atcggccagt tcgggtacgg
     7381 cgccataaag ttctccgggc gaaaaggccg gaattacagc gccggtgcgg tagtcgagac
     7441 gcgaggctat ggtgccgccg gtgccaatca gttttacttt gggcttaccg ggagtatagg
     7501 ggaactcctt ttcgggtatt ttataatttg cctttttata acctacttct ttcatcgtca
     7561 ggatggtgct aatgtcaatc ccgatgttat aaccggtaac caccttgagg acaatgtgtt
     7621 tatcatcatc attttcggcg cggggcagaa cggtcccttt aaacaatccg cgggtagtat
     7681 ccacttcggc ctgaccccat acacggacgt tatatttttt taatatttca agtgcatcgc
     7741 ccttatatcc ctgaaaaaaa tcttcgctca ttgtagtatg ttttttagct gttaattact
     7801 ggtttcaact aatcctttta attcggccag acaaatgttg ccggtagcca ttttgcgcaa
     7861 accacccatt atccatttgg tttccacatc aggttgttgc gaaatgcgta tctgatggaa
     7921 tttttctttc ataaaaggaa tattggaaag aatctcttcc ttggtgattt ttttgaattt
     7981 gatagtggca agaattgatt caaaatccat cttaggatgc tgtatcagga tgggcagcat
     8041 tttttttgca atctgtttat ccagtttctt ttcgcttata aaggcaaaca tatctccaat
     8101 catttcataa ttaaagtatg gtgatatagc atgttgtcct tcaaaatatt ttagggtgtg
     8161 ccctataaat gttccgataa atttgggggg atagccatta acgctgataa ttttttcaat
     8221 taccgggaaa agatttttct tgaatatgta tgtgaacgta tcttcaggta tgccccattc
     8281 tttgagtttc tggtatcttt caatgacttc ggtgggcagg tttttcttca ggcggttgat
     8341 atattcatct tccaggggga taggcaccga atcagtatcg ggatacatac ggtcggcgcc
     8401 tggcagtacg cgttcaaaaa tggtggtgcc gttttcgaat gatttgcggg tttcgttagg
     8461 cacgcctttg aaagcccatt ggcagcgttc ttctatcgtt tccagtgcgg ttttcatatc
     8521 gtctgccgga ccccagagta taatctgtgc atcatcttct ttggcgttta acgaggcgcg
     8581 gatgtttgag aaatcatcac cgcttatcag gggctcaaat tcttccgagt gggtcatgtt
     8641 gggcagctca atacaggcaa tcactttcag gcgttcgctg atttcgtcgg caaacatttt
     8701 tccgggctgg gtgaaatgcg aaagtatttt tttgaagccg ggaaggttga tggcataata
     8761 cttataagcc ttcttttttt cttcgttgag gggcaggttc gaaaaatgaa aatctttgag
     8821 ttgtacttcc tgataattca tcttccacga ggatccgtct ccgaattttc cgtttaaaat
     8881 ttcgcggata tgtaacaagg cccattgtct gaaacactca ttatgcgtga gctttggtat
     8941 ccaccgtgta tgggccacac ctttgatttc tatacgggta ccgcctttac aactgacgtt
     9001 tacatcttgc cggccggcgc cgatacctgt tcttaccttg ccggtgctgc ggtttaaaaa
     9061 gcggatataa tcacaggctt cttttacctc gtaagggttt accatatccg gataggtaac
     9121 ggtttcaatc aaaggcaccc cgagacggtc ggtggagtaa atacggcggt gtccgatatc
     9181 ggaaatttca cgacaggaat cttcttcaat actgagttga atcaacctga tgttttttgt
     9241 tttcaaaggc agttcgcctt ccacgcccaa tatcgccgta cgctgaaatc ctgtaggaat
     9301 gcttccgtcg aggtattgtt ttctggtgat gtgcacttcg cctacgatat tcaacttgct
     9361 gagcagcgca atttccaaag cgttttcgag ggcttcccgg tttagtttaa atggaggtgt
     9421 atcgtccaca tcgtaagtgc aggtcgtttc gttttttacg cgatacacaa tttctttgcg
     9481 tgttttaaac tccatcaggg cggtgccgtc gtattcgccc agttcgctaa gtgttgggcg
     9541 catgtgcctt agcagctcgg catcgtactg attttttttc tggtacaccc ccgcaggaca
     9601 atggcaaaaa agtttttctt tggtcttaag ttgctgatga acttccagtc ccgacataaa
     9661 gcctatgcgg ttccagtctt cctgtgttga ttgtttcaac ggcgtatatc caatggcttt
     9721 tttagtttct tcgtagttta gctttggatc gaaattttct ttcataattc ttttattgta
     9781 attgggcggt caaaattagt catttaaatt gtttatccca tattactcaa taaaaaattt
     9841 ataccggtgc aataaaagaa accagcatag gcaggttcac tggtctgcat catgcgccag
     9901 gcaggctttt tcaacagtat atgaatatcc tgaagctatg caggccacta tttacagatt
     9961 tgattttgcc caggctaaat tattttttgc cagttcgtaa cttggattta ttttcagggc
    10021 tttttcgcag gcttcggcac ccttatccca catatgcatg gcattgtaag ccgaacaaat
    10081 gttgttgtat gcttcggcgt aatcgggttt aagtttcagg gcttcgttac aggcttcaat
    10141 acatttctcg tactggccca gatgataata ctgaagactt aggttgagat agttttcagg
    10201 ggtgggattt tgccgggcaa actgcaagtg ttggtctgct gcagaaactt ttttaactgc
    10261 agcctcacga tatttcaggg ctatggtatc gccgccggaa atggaaagtg tgtgtagcgc
    10321 caccgaatcg agcttttccc agagctccag cctgttataa atatccatca tctggtaaag
    10381 gccctgcatg aatatgggat tgatgagcag atcctgttct tccatccggc gggcttcctg
    10441 gattcggttt tgcttcaaca aaaatcctgc ataataataa tagggttcgt tgtactgcgg
    10501 accatattgt atggctttgt taaaatatcc ttcggcctct gccggttttc ccatggcatt
    10561 ttttaaaatg ccgaggttaa tatgcaaaaa tgaatagtat ggcgtatatt cgagcgcctg
    10621 ttcaaaatat ttttcggcaa ccggataatt ggctttagcc atttgcgtca ggccataatt
    10681 cataaggccg cgaccatttt taggactttt aatggtaacg tcaagccaga gcgattcttc
    10741 gtctttccat acttcgtttc gctgatgcgt accataagca aaggcagaca aaatcagaaa
    10801 aacagccacc cacaacacaa ccagattctt tctgtttttt ataaaaatgt gttcattttt
    10861 aaccaccagc aaccctaccg tgtaaattac gctgaatgta agtccggtga aaggaagaaa
    10921 catcctgtga tcgtttagca cttcggtgag cggaactaag gatgtgggca acagcgacac
    10981 ggtaaaccat atcaggccaa aagctattgg ccgttgctca gcctttgaag ccgttttaaa
    11041 aatcacaaaa accagcacta tcacaaaaat cactcccacc attatccggt cgtcaaaagg
    11101 attggaaagc actgtccagt cggtatcggc acttaggttt attggcagaa aaaatgaaag
    11161 aaaataatgc acccagacaa aagtttgtgt cagcacatat ggcagtaccg gattgtcgat
    11221 attttcaatg ccttccctgg gcattttcgc ataagaatag tattgtaaaa ggcctatgat
    11281 aattaatatg ggaacaaaca tcaaaactgt tttacccaag gttttcaaat tgcaggcctt
    11341 aaacaggtcg gtcagggaaa gattcttttc gaagaaaatc acataaaaga ataacaagac
    11401 aggtatgatt aaggcggttt ctttaaaacc tacgcaaagt attgccggca atatataaat
    11461 accccatttc cgcaggtggg gaaaaagaat aaaaatagcc acagaggcaa tgatacacaa
    11521 ggtggacagc acgtcggagc gtgaaatgat ataattgatg gtttcggcgt tgacagtgtg
    11581 aaacgcaaac actgcagcag caacaagtgc tacatagccg gcccaggtat gtttaagtgc
    11641 tgttttactg atttttttgt aaacaaaaaa taaaagtatg caaagcagaa caaaccacag
    11701 aaaagtggaa agatgaaaat aaaaaggttt caaccctccg cccagccaat aatcaacggc
    11761 gagcgtggtt gttaccatcg gacggtagcc ctggtgcgaa ggcatggagc tgaatgtttt
    11821 agtgtcttta aaaaacaatg gaacattttt caggctggtg atgtaggggt tatcctgtat
    11881 ggtgtgcgaa tcgtcaaaat gaaagccatt attgaaatgg tttgaatacg taattaccaa
    11941 ccctgctgca aaaagaattg ccagggcaat aaggtacaac cgcgtttttt tcatattaag
    12001 gtgacttaaa aatcacagta aaaataaata ttttccttga aataatttta accctattga
    12061 gattgtgatg tgctgtaagc ggtaacattt catcacccgt gtcgcggaaa aaatagttaa
    12121 ctttacaggc cttttatcca aaagggcaat cctcattata tcaaaatgaa aactcaggat
    12181 aaagaatatg ccggccgtct ttataaaaac caatatgtgt ggtggaaacg cctgctggat
    12241 gtgcaaagac cttaccggtg gaatttgcgt cgtttaaaac cgggctttgt tcttgacata
    12301 ggttgtggca tcggccggaa tcttattcat ctggatggca acggaatagg tatcgatcat
    12361 aatttggctg cggtggaaat agctaaaaag aaaggcttac aggtttttac gccggatgaa
    12421 tttcacacga gtgaatacaa tatccctttg cgttttgact ccttattgct ggcacatgtg
    12481 gcagagcata tgacagaaaa agaggcgata aaactcctgg aaatatattt tccattatta
    12541 aaaaacaatg gaaaccttat tatcattact cctcaggaaa caggctataa atctgatgca
    12601 acgcatgttc agttcgtcaa ctttgacgtg cagcgccgga tcattaaagc gttgaatctc
    12661 gaatttgtaa gacaatattc atttccgttt ccccggtttt ttggaagttt tttcaaatac
    12721 aacgaattca taagcatcgg ccgcaaacag gcgtgaatct cattccaggc acacaaagct
    12781 acagccgctt ctcttttttt tcttttctca gggcaaaaaa catggtaaca aaatctttta
    12841 atacaataaa agttttgagt cttattaata tttttatgaa gtaccccggc ctgatgaaaa
    12901 acttgttgac agccatatca cgcagttggc tgagttgttc ggcggtcagt gtgttgttta
    12961 gtgcgatggg aggggtattc acactgccaa agtcgttcca tggtggcaag gagatattgt
    13021 tttcgaggca atgttggtac aagcctgttc ccggcagcgg aatggcagta gcaaaagagg
    13081 cataatcctg ctggagttcg caggcaagtt ttatggtgtc tgatgctgtt tcgtaagttt
    13141 ctcccagaca gccgataatg taatttccac ccacaaacat accggcttta cgggcatttg
    13201 caacggcgct gcgtatctga tcgaggcgga tgttcttatc catttggtca agtatttgct
    13261 gattgccgct ctcgattcca aaaaagaccc agcgcacacc ggcttctcgc atctttttta
    13321 atgtaggcag atgggccgta ttggctcttg aggagatttg aatttccagc cgtttgttat
    13381 atcctttttc aataatataa tcgcaaagtt caatcacccg ctttctgttt agattaaacg
    13441 tgtcgtcctg aagatataaa atgcgaatgt tccactcctt aataaaaaaa tccagttggt
    13501 catacacctg ctgcatatca aaataacgca ccttggggcc aaagacagtg tgggaagcac
    13561 aaaaattaca acggcctatg catccacggg ctgtgaaaat cacgccaata ggaccgtttt
    13621 gacccgacac ccagcggctc cataaatagg acctgtaaag atgttttttc agcagatgat
    13681 tggccggtac cggaagacac cccagattgg ctattggagg gcggtacccg gtaataacaa
    13741 tcctgtcgtt ttttctgtaa acaataccat caacatcctg caaagacaac tggtttttat
    13801 atgcattgac aatatcaata atagtaaggt cgccctcgcc aataacaccg atatccaaag
    13861 cccatgtgtc ctccagtgtt tgcttgggca gcgccgacac atgcgggcct ccggcaatca
    13921 tcactgtctc aggtgttttt ttcttcagca gagtgagaaa ctctctggta atatccatgg
    13981 tagctgtcat aatggtggca ccgacaaagg caggtttttt ttcagcaacc atattgacaa
    14041 gcttttccat ggtgaggttt tcggcatcgc cgtcgatgat ggaaacatca cagcccgccc
    14101 gtagagctgt ggcggcaatg ctggtcaggc caatgggcgg gcttacagaa acaaaatgtt
    14161 ttaggttatg gtaagcattt tccatggtag gtaccacaac taaaatcatc cggttatttt
    14221 tcatagccat cctacaaaat attatcttta tgacttaacg aataaatgcg gcccagcata
    14281 ccggcgatac gctttcccca aataattccg aaaattctta attcttcttt ttttctgacg
    14341 ttggttacta actgtctggt ggtttcagaa tcctcatgga tacggtgttt catcagtttt
    14401 ttattgataa aaacaaacgc cccgtcacgg ttggccatat ccagccaggc cttccagtcg
    14461 aggctgtatg aaaaatcagg tgaaaatgcg aaatccttta ggtggttttt attgaatgtc
    14521 accgaagggc aacaaattgc gttaccaaaa atcaaaggca gccgtttgaa aaatctgttt
    14581 tttatgcaag gctttatcag aaagggaatc aacaaaattt ttttggtaaa aaaattcaac
    14641 gaatgcctgc ggatttttcc atgaatgatt tcgcagtaat cagtaaataa aatcaaaact
    14701 ttattgttgt ttttttccaa gacgttttcc aaataattgc tttcgtaaat atcgtcctga
    14761 tgagcaagag taacccatgt tgtctgggcc tccgacaaag cgaaattcca atcgcccgca
    14821 atgtccttgc tgttattgtt gaccagcaaa ggcagcgaat gccgttgagc tacatccttc
    14881 aaatgttgcg atggggtaga agtggttatg cagatatgac tttttttggt ctggtttttc
    14941 agcgagacga tacattcttc aataaaaaca gaatctttat aagccggtat aacaaaagta
    15001 tggtcttcca taggcatcaa atgtatctga aaaatttccg gacatctgcc agtaaaattt
    15061 tcggctccct ccggtacacc gaggttttag ccagggctgt ttttataaca tgccgtggtt
    15121 ttttattata acgatgaaat ttttctttaa tatcactgac cacctgaata aaagtggctt
    15181 cgtcaagctg gtcgctgcgc acatgagggt ttttgatata ataaagatgg ggcagggtgg
    15241 aataaaaatc ttcaagtagc atgggggtct ttaccatgtt tttttccaag ccatcatcaa
    15301 acattttggt cccgggaaaa gtttcgtaca ccccgagcag cgccaaatca ggcttcagtt
    15361 catacataaa ctccagcgta ttatacatgt cttctttggt ctcgcccagc atgccaataa
    15421 gaaagtaagc actccaatgg atgcctgatt tacgcaggat ctttgctgca tttctgcttt
    15481 gctcgcaagt gattcctttg tttatttttg ccagaatttt gtcagccccc gattcgatac
    15541 caattttaat ataattgcaa cctgcttttt tcatcacacg gagcagttct tcatcaataa
    15601 ggtttacgcg ggtattacat tcccagcaga tatgcaattt ttcgccgatc agtctctgac
    15661 acagctcata gactctgctt ttgtttattg taaaggaatc gtctttaaaa gcaaactggg
    15721 tggtgccgta tttttgtttt accagtttta tttcttcaat aatgttatct acctcacggg
    15781 agcttatttt tttgatgtgt gaagcgcaaa aagtacagtt atagggacac ccgatggttg
    15841 tcataatcaa ccccatatct tccgaactgt aactcttctc attcatcagc aacgaccggt
    15901 caggaaaaga cgttccggaa attctttcgc gggtatcggc tgccggatta tgaattatct
    15961 gtccatccat tcggaaagat aatcctgcga tagactgcag gctgccttct ttgcgctgaa
    16021 aatggttagc taaatcaagc agcggctttt cgccggctcc tttcaccaca aaatcaacaa
    16081 aaggggctat ttgcataacc tccccggctt tggatgtggc atgtggaccg ccaagcacaa
    16141 cgggacaatc aggataaatc gttttggcaa tttcggcggt gtgaaatgct gaagccgcaa
    16201 aagtagtata gatagatatc ccgataaggt cgggacaaaa atcgctgatg gtttttttta
    16261 cctcagccca tacagggtgg tctgcatcct gcaacgaact tagaaaaaca ggatattttt
    16321 caggcaacag cgaataatcc atgttttccg gggtgttgta caaatcggca tcatatacca
    16381 aagcctcgtg cccggcacat ttcagcgtgg tggcaagcat ggtcagtgcc agaggaaaat
    16441 accggttata aaaacctatt atcctgtaaa aaggcggatt gaccagtaat attttcatta
    16501 ctttagtctt tcctttctga acatggaaat ctggtcggcg attaatccca gaaatacaag
    16561 gattaagcct gataggatac tgatgatagc accgttgctc acccccctgc ccataagcaa
    16621 aaatggtagc ccccaggcaa tgccgaatat aatacacaac agtgcaatag gtaggaaaaa
    16681 cctcatgggg ttaaacagca gcaggatatg gataatggcc agaatggtgg aataagcggt
    16741 gcccagcgaa atcttgcttt tgccctcccg gcgttcacgt atccggatag gctcttcaat
    16801 taccaggtgt ttctggttta taaatatcag ggcaataaaa tcgctgtagg ccatgtcgtc
    16861 cgggcataag ggaatgtatt tctgggcaag agccgtatca cacagtttca ttcctgaatt
    16921 gatatcatag attggtaccg tcatagtcat ttttgcaaaa ctccggatta tcgacttgcc
    16981 aaaatgccgg taataattat ggatcttctg gcccttgcgg ctgccaatga ccatgtcggc
    17041 ttccgtctct tttgttttat caagtaattt caacacgtct tccagataat gctggccatc
    17101 ggcatcaatg gtaatcaggt atggcgttgt ggtgttttga atccctgtct taatagcgcc
    17161 gccataaccc ctatttacct tatgatgaat tatttcgaca aaatcattac caaatgtcaa
    17221 atcctttaat gtttcatagg ttttatctac ggagccgtca tcaacaacaa tcagttgaaa
    17281 accctgttgc ctgcaaaacg ctataacctc cggtaaaaaa gatctcagcg aactttcctc
    17341 gttataggcc ggtataatta tagtaagctg tttctccatt tcggcttttt tttacgaaac
    17401 atcaaaaatt atgaaatcgt ctaaaaatac ttatactcga tttaactcaa acaggataca
    17461 aatttaacaa aaaaacattc ttttcaatag ttttgataat atgaaaacaa gtatttactg
    17521 accaatgcat gcagcatgac atgtaaaaac aaaatatccg tctgtatgta aaaatatttt
    17581 ggtgattatt tgtaaaaatg attttctaat gaaacaattt ttatttttgt gttataaaca
    17641 aaaaacaatc cgtcagttaa aaagaaaaaa cttaaaacaa aaaagataat acagaaaaaa
    17701 ataaagtttt tttgattact aataaatttt tataatttcg taatataatt ggaaaaaata
    17761 ttgtcaaata gacgaaattt gcgtatattt acattaacca aattagtaag gtaatgaaag
    17821 aaaaatcagg agataaatta catatagtcc ttaaagagtt tttcgggttc gacacgttta
    17881 aaggaaatca ggaagccgtc ataaaaaatg tattggacgg caaggacact tttgtcatca
    17941 tgcccacagg tggcggcaaa tcgctcacct accagctccc ggccctcata agcgatggta
    18001 cggctattat catttcgccg cttatttcgc tcatgaaaaa ccaggtggac aatatccgga
    18061 attttggcgt ggacaaaggg atagccaact ttttaaactc ttcactttcc aagcaggaaa
    18121 tgaccgatgt aaaagccgac ctgcttgcgg gaaaaaccaa actgctgtat gttgcccccg
    18181 aaacgcttac aaaaaaagaa aacattgaat tttttaaaca gttcaaaatc tctttttacg
    18241 ccatagacga agcccactgt atttcggaat ggggacatga tttcagaccg gagtaccgca
    18301 acatcaggcc taccattaaa gctatcggca aaaaagttcc tgtgatggct ctcaccgcca
    18361 ccgctacgcc aaaggtacag cacgacattc aaaaaaatct gggcatgatg aatgccggac
    18421 tcttcaaatc atcgttcaac cggcccaacc tgtattatga agtaagagca aaaaccaagg
    18481 acgtacagcg cgacatcata aaatacataa aaagcaacag cggaaaatca ggaatcatct
    18541 attgcctcag ccgtaaaaaa gtggaagagc ttgcggaaac tctccaggtc aatggtatca
    18601 aagcgttgcc gtaccatgcc ggcctcgaat ccgaagtgcg caaagccaac caggacaaat
    18661 ttctgatgga agaagttgat gtaatcgtgg caactatcgc ttttggcatg ggtatcgaca
    18721 agcccgatgt gcgctatgtt atccatcacg atatgcccaa aagcctggaa ggctattatc
    18781 aggaaacagg ccgctcagga cgtgacgacg gcgaaggcaa ctgcatcgct ttttatgctt
    18841 atgaagacat ccagaaactg gaaaagttca cctcgaaaaa atcggtcaac gagcaggaaa
    18901 tagccaaaca actgctcatg gaaacaattg cctatgccga atcttcggtg tgcagacgca
    18961 aattgctgct gcattatttc ggcgaggtct ttgaggaaga aaactgccag tgttgcgata
    19021 actgcctgca tccaaaaaca aaatttgacg gcaccgcata cgtagaaaca gttttggaag
    19081 ccgttgtgga aaccaaggaa caatgcaagg gcgcacatct ggtaaacgta ctcgtcggca
    19141 aagtttcagc ccaggtaaaa tctttcaaac accataaact taatgtattt ggtaaaggtg
    19201 ccgatcatga tgaaaaattc tggaccgcgg tgatacgcca aaccattgtc aacgggctgt
    19261 tgagcaaaga tattgaaaac tacgggattc tgaaaatcac caaagaaggg gagaagtttc
    19321 tggacgaacc ggaaactatc atgcttacac aggaccacga ctacgacaca ccggaggaag
    19381 atgaattttt cgccggcgga ggaaaaacca gcacagcgga taaaacattg tttaccctgt
    19441 tgaaagatct ccgcaaacaa atctcccgca aagaaaacct gccgcctttt gtcatcttcc
    19501 aggacccctc gctcgaggat atggctatac aataccccat caaaatggat gaattgatgc
    19561 aaataacggg tgttggttcg ggcaaagcac aaaaatacgg aaaacccttt ctggacctga
    19621 tagcaaaata tgtggaggaa aatgaaatta tcaggcctat ggacatgatt gtcaaatctg
    19681 tcgtaaacaa gtcgggactg aaagtgtaca taatccagaa cattgaccgg aaaattcctt
    19741 tggaagatat tgccaacgcc aaaaacctta caattgaaga actgctgatg gaactcgaaa
    19801 gtattgtggc ctcaggcacc aagcttgata tcaactatta tattgaggaa tatgtcgagc
    19861 cttatcacca ggaagaaata tatgactatt tttatgaagc agaaacggat tcggtggaag
    19921 acgcactcaa agaactcggc gaagaagaat tctccgagga ggaaatacgg cttgtccgcc
    19981 tgaaattttt atctgaagta ggtaattaga aaaatttaaa aagtacaata atgaacgaag
    20041 aaacacgtaa tgaaaaccag gagccaattc ctgcctctac tgacgaattc attccagcac
    20101 ctcaagacaa gcctgca
//
LOCUS       DAOSFP010000004        18518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_2445_length_18518_cov_4.167958, whole genome shotgun sequence.
ACCESSION   DAOSFP010000004 DAOSFP010000000
VERSION     DAOSFP010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18518
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_18518_cov_4.167958"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLI16_00315"
     CDS             <1..868
                     /locus_tag="PLI16_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOH83031.1"
                     /translation="SGRALTLYEPMSAHAQGFLSPENLDIYIKLFDSALMASEGNTAY
                     CHRIRMARQPVLYAWLEVAKSLPFTDNWLFEKDEKGMHSAREKARQYLRELCDTATVY
                     GPMLFHEISIKPQEYYERMSTYFENGVQMHKAVGKSISFATPASPKYAANGPNSLIDG
                     VCGTDNYFSLWQGWYGEDVDATIDMGSEESISRIQLNCLVNQASWIFAPANITVRASN
                     NGKDFIEIMTFINADASKKMDKSIVPFLIEFPVPQNCRYVNIKLNTIGNLPEWHGSEG
                     KTWIFVDEIMVK"
     gene            1171..3378
                     /locus_tag="PLI16_00320"
     CDS             1171..3378
                     /locus_tag="PLI16_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOH83032.1"
                     /translation="MPYLKQCLYCLALLIVCCLTTNPVFSQSKISIIPKPQQVVLMKD
                     SFALHQARVVLPKETKALRVATFFTVAVKQQTGINLLQKKTLNTICFSYSKKITQAEG
                     YKIDISSKKINITAKNDTGLFWAVQTLRQLLPDSYGTGVFIPCMQITDAPLYGWRGNM
                     LDVCRHFFTVEFIKKHIDMLSYYKINTFHWHLTDDQGWRIEIKKYPGLTKVGAWRKES
                     QDSVYGGFYTQEQIKEVIEYAGQRNITVIPEIEMPGHCLAALAAYPELSCRKIKFEVA
                     NYWGVMNDVYCAGNEKTYQFLEDVLTEVMALFPSKYIHIGGDEAPKYRWQQCPDCQKK
                     IQDEHLKNEAALQSYFIKRIQQFLQSKGRILIGWDEILEGGASPEAIIEVWRGEDKAK
                     EAIDNKNKIIQTLYIDAPMSSLTLSKTFHFDPAVKENNEYVLGAECPLWAEAVTEFNT
                     DYMMYPRLQAFSEVLWSGKTSYDDFLSRIKKHYSMMDKQGILYGNENKPMLEVNLKYL
                     PESKCWKIYEKHGNGDIVIHYDFNGKTPGNPSPVFSDSVTIQKPQTISVIAFHKNAPA
                     SLPVTIKIEDNRATGIKPVYKTEYSEKYNEGGVYGMTDGITGTLKYGDGTWLGWEGKD
                     LEMEVDLGLETNLTYLQINCLQEVQSWIMLPSDIEFYISNDNINWQKLATLSHSVSDQ
                     DLKPQIYNFIYKAPQPVKARYVKVIAKNYGKLPVWHNGAGGNSWVFADEFIIR"
     gene            complement(3529..5559)
                     /locus_tag="PLI16_00325"
     CDS             complement(3529..5559)
                     /locus_tag="PLI16_00325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOH83033.1"
                     /translation="MKKTLVSFLLAVAFIPALFAQGRLTPELLWKFGRVSDIKLSPDK
                     KSILYGVTFYNLEQNKGNRDLYLVPLAGGAAVNITNSPGSEWNGVWRPDGKKIGYISA
                     EYGTPQLWEMNPDGTSKKQITSFEGGITGFAYAPDMKHILFTRDIKLDKTVTEVYPDL
                     PQADARIIDDLFYRHWNDWHDYAYSHIIVSAYADGTVTGGTDILGTEKFDAPLTPDGG
                     MEQIAWSPDGQKVAYVCRKLHGKDEALSTNSDIYLYHLQSGLTENISKDMGGYDLDPV
                     FSPDGKKIAWCSMKTPGYESDKKRLIIYDFSAKTIADMSANFDESASTLNWSADAKSI
                     YFLSYTKGTCQVFQCDMATGKINQLTEGVHDFSELSADGKNTVAVKMSMSMPSEIYRI
                     DNEKGKYKPVQLTFTNKELLSSITMGNVEERWVTTSDNKKMLVWVIYPPNFDKNKKYP
                     ALLYCQGGPQSTVSQFFSYRWNFQMMAANDYIVVAPNRRGLPGFGKEWNDQIALDYGG
                     QNMLDYLTAIDSVAKEPFVNKDKLGAVGASYGGFSVYWLAGNHNKRFKAFIAHCGMFN
                     FESWYGTTEEMWFPNHDFGGAYWEHPKPQSYDFSPHKFVGNWDTPILVIHGGYDFRIP
                     YTEGMQAFNVAQLQGIPSRFLYFPNETHFVTKPQNSVLWQREFFGWLDQWLK"
     gene            complement(5649..6671)
                     /locus_tag="PLI16_00330"
     CDS             complement(5649..6671)
                     /locus_tag="PLI16_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83034.1"
                     /translation="MKKVCYLSVIISLLILFACGNHQSTEQGIANTDSLPVNLAEITK
                     KIEADPQNAELYNQRAIMYLDRKETDKALTDINKAITIDSKNSLYLRTLSDIYFATGK
                     LRNCQEALNKAIEMNPDDAEAVLKLAELYYYQKDYKKTFEFADNALKIDKLNAKADFI
                     KGMAYKDLGDTAKAVRSFQIAVEKDQEYFHAYMQLGLMYSVKHNPLAIDYFNNALNIN
                     PQSTEALYSLALFYQENNEYNKAIEKYTTILQLDPKNKYAHYNLGYIHLVYLQVYNVA
                     IKHFSDAIMCDPTYAEAYYNRGYSYELSGNVMAARNDYKKALEIRVNYQKAIEGMNRL
                     DNISRK"
     gene            complement(6808..7833)
                     /gene="recA"
                     /locus_tag="PLI16_00335"
     CDS             complement(6808..7833)
                     /gene="recA"
                     /locus_tag="PLI16_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOH83035.1"
                     /translation="MAKEKETEEKKDNALNKEKLKVLQLTLDKIEKSYGKGAIMKLGD
                     HPAEDIEYISTGSLGIDAALGVGGFPKGRVVEIYGPESSGKTTLALHAIAESQKKGGI
                     AAFIDAEHAFDSSYARKLGVDIENLLISQPDNGEQALEITENLIRSGAIDIIVIDSVA
                     ALTPKSEIEGEMGDSKMGLQARLMSQALRKLTATISKTRCCCIFINQLREKIGVMFGN
                     PETTTGGNALKFYASVRIDIRKVSQIKDGEAVTGNRVRVKVVKNKVAPPFRTAEVDII
                     YGEGFSKMGEIVDLGVEKEVIKKSGSWFSYNETKLGQGRDAVKQLLLDNPELCEELES
                     KIREKIS"
     gene            complement(7850..8317)
                     /gene="bcp"
                     /locus_tag="PLI16_00340"
     CDS             complement(7850..8317)
                     /gene="bcp"
                     /locus_tag="PLI16_00340"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOH83036.1"
                     /translation="MDTLKKGDKAPDFTGKNQDGKEVSLKDFSGKKLVLYFYPRDNTP
                     GCTAEACNLRDNYQLLLDKGFAILGISADDEKSHTKFITKFQLPFSLIADTDKKICKL
                     YGVWGPKKFMGRTFDGINRTTFVISEQGTIEAVFNKVDTGNHTDQILNELKIK"
     gene            8556..9953
                     /locus_tag="PLI16_00345"
     CDS             8556..9953
                     /locus_tag="PLI16_00345"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOH83037.1"
                     /translation="MPDYLFEVSWEVCNKVGGIYTVIASKAPGLTKKLKNNYLLIGPD
                     VWKETPVNPDFIEDKFLYKSWREDSEAKGLRFRIGNWNIPGRPVVILVDFTQYFAQKD
                     DILSRLWEKYQVDSYTGQWDYIEPALFGIAASRVIQSFYEFHVIASDHIVAQFHEWMT
                     GSGILSLKGRVPQIATVFTTHATILGRSIAGNGLPLYSNLTEYDNDVQAQKMNIMAKF
                     SLEKNSAKFCDCFTTVSEITARECAHFLKKEVDVLTPNGFDHSLVPENSDFDQKRTTA
                     RETLKKVTEGLINQPIDDNAVFLLNSGRYEFHNKGIDLFIDALGKLNREGNLPHTVVA
                     CLSIPAYHAGPSRELSQRMEKRDFGQAVSYDYCTHYLHNYDKDAMISRLKANHLNNSP
                     SDKVKVVIIPCYLDGKDGIVNLNYYDFLIGFDLTVFPSYYEPWGYTPLESIAFHVPTI
                     TTSLAGFGLWARSQG"
     gene            9969..12797
                     /gene="glgP"
                     /locus_tag="PLI16_00350"
     CDS             9969..12797
                     /gene="glgP"
                     /locus_tag="PLI16_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOH83038.1"
                     /translation="MTVIERDDNNAEFVTGMVAKKIVDFVNSTAKEKDTARQNAYELS
                     KMALWDELLSNYYQAYHNALEKAGNRYEEYKSKSLPVQYEIIPQKQLNPKWNKVLVRP
                     SIIEKLSPLYKLTQNLWWTWNLEAIELFEMINPELWEQCKQNPMTVLESLSLEDYKML
                     VKDTVFREKLDKVFKKFTDYLAYGPKKAKEQVAYFSMEFGLHHSLKTYSGGLGILAGD
                     YLKEASDSNANIIGVSLLYRYGYFDQSISLLGDQVPSFHPQKYSHLPVIPVRDKKLGA
                     DGSSTWVKIQIAAPGRIITAKVWRVDVGRAALYLLDTDIEENTAEDRTITHCLYGDGS
                     ELRFKQELLLGIGGIRLLERLKISPDVYHINEGHAAFSVIERLRKCVQEENLTYQEAL
                     EVVKASTLFTTHTPVPAGHDRFSEDILRAYLHHYADMLKISWDTFMNLGKAREFDPTE
                     EFSMSYLAATLSSETNGVSRIHGSVTRDMFAELYPGYFPEELHISHVTNGVHFPTWVS
                     KRWKKLYDSVFDPEYINDLSNHEYWKKIYGVPDETIWKIRQEQRSELINFLRSRLINE
                     MTLRQDNPQNIFNVSQSLNPKALTIGFARRFATYKRANMLFSNLDRLSKIVNNPEYPV
                     QFVYAGKAHPKDVEGQKLIKKIYEISMMTEFLGKIVIVENYDIELGKKLVQGVDIWLN
                     TPTRPQEASGTSGEKAVMNGVINFSVLDGWWAEGYRPEAGWALKEERTYKNQAAQDAL
                     DAATIYNMLENEIAPMFYKRTKKDIPLEWVQKIKHTIALIVPEYTTKRMLDDYRDKYY
                     RNICKRSIDINSDNYLNAKKLAAWKQKVFRAWDSIEIVSVTIPDSNKKPLSLGEKFYA
                     EIKIKLHELNKNDVGVEMVFGQKSDDVVNRILFQRELKIKNTNHNVITYSIEFPSVRI
                     GVFDYAFRIFPKNPLLANRQDFSLMKWI"
     gene            complement(12991..14238)
                     /locus_tag="PLI16_00355"
     CDS             complement(12991..14238)
                     /locus_tag="PLI16_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH83039.1"
                     /translation="MKKKNLLLTFLLYFSLMFFCKIDFAQQFSFVHISDIHVSDGTLG
                     GSVGNNDSDGEQFQCTLKEINYLNPKPAFVVASGDISNVGSIHPDGMYDALTQHLYPQ
                     GGITNPANGAYFVDSAMTIPIYFVSGNHEYYKTIVPPVINDNLLFYPLYLGQDMDYYF
                     TYNDAFMVFIRSGADRPIWQDLHPACVESEGIAAAQCQWLRSTLSLAGNKRKIIVMHH
                     PPVNVNGTEIDGSPNTGTIAGPDDGSFLYNRETFMNICDSNNVDVVLAGHVHQFVVAD
                     RQGNVVGENWTASTRYCQTAQEFEGAYRIITVDSNFVSIGLPSVVNCLTVAVNNYEKK
                     DYNIYPNPFINSTTLELPNFSDNAHYELRIYDMMGREVMYIPDIQNGKNTINRGSLLA
                     GMYFYSLTDTKNIIGSGKLIIKD"
     gene            complement(14383..14940)
                     /locus_tag="PLI16_00360"
                     /pseudo
     CDS             complement(14383..14940)
                     /locus_tag="PLI16_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745397.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(14942..16075)
                     /locus_tag="PLI16_00365"
     CDS             complement(14942..16075)
                     /locus_tag="PLI16_00365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83040.1"
                     /translation="MKKFPAFFWMIFSVGMAVGQSNYNHGNEAYINEKWLGFNPVTIK
                     NGSAVYNYDTFNKVYRCGDHILIPDKTSIPDGKRVHVNAFFGYGVGGNISDTAFNWDN
                     QTILTPNDFNRSKLFVSPVSDVGMTYAAALQYNSPYCNYDDCFATIDGPVSGLAYPTF
                     LPDSGIYDIWDGEELINTGDLKNTGTMQKVSQQQTLTTANRSYCVNKYGEGWRLPTDM
                     EVGHFNDNEGTGWGLDSAYMGISPYYIWTSSLYKIYTVKRWATRITDGYWENCGGFLY
                     TTNHLRCVFEGVENTSTKVEEPLLIDEKIVLFPNPCKELLIIKNISGATVKIYNSQGV
                     EVLTDGLSSSQRTFDLGCCPNGLYFVRIISGENQLIRKLIMAR"
     gene            complement(16090..16476)
                     /locus_tag="PLI16_00370"
     CDS             complement(16090..16476)
                     /locus_tag="PLI16_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH83041.1"
                     /translation="MKKILCVLLLMTFSCYLFSQETGKPVIEFETLVYDFGIINKGEK
                     AECVFTFQNSGKLPLIITEVKASCGCTVPTWTKEPVKPGTKGSVNVKYNTNTVGVFSK
                     TITVNSNDNVRRTVTLTIKGEVKKKN"
     gene            complement(16478..17443)
                     /locus_tag="PLI16_00375"
     CDS             complement(16478..17443)
                     /locus_tag="PLI16_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83042.1"
                     /translation="MIGRTLAFGFLWMLCAQGAWAQCFASAGNPVGGTANLGVMDKKT
                     FRLGMFYRFSYSHRYLTGDKKYTGSAGILKDANFNYLGLLIGYGILDQLTVEAESGYY
                     INKTQVYKLNNLKLKGYGLSNAVVSLKPRLYYNADKRFEISCALGANIPFTHNFQAVD
                     GVTLPVDLQASTGSFGMVFQTFIIKEYSFEGIRFFLTNRIEKYFINKQEYLFGTMYST
                     SFFFSKHFVFEKLKLKDWTFIFQLKNQIKEKNKHFRQPVDASGSVLLFVVPQVNVSIN
                     DNWNVSVMADIPVYQYYNKIQLANTCSVGLSVFRDFSFEKKQKLE"
     gene            complement(17440..17769)
                     /locus_tag="PLI16_00380"
     CDS             complement(17440..17769)
                     /locus_tag="PLI16_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83043.1"
                     /translation="MKKLIILFVALACMFSACKEENDGSLLNSFISLTAENDTIITGH
                     STKITAVVDGENLTFLWSATAGDILGSGNVVDYVAPTCTPGNNQVSCTVEASNKSETK
                     TITITVL"
     gene            complement(17780..>18518)
                     /locus_tag="PLI16_00385"
     CDS             complement(17780..>18518)
                     /locus_tag="PLI16_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibrobacter succinogenes major paralogous
                     domain-containing protein"
                     /protein_id="HOH83044.1"
                     /translation="TDITGATSNNFPLVPSQSYFYRAKVNSGTCLPVYSDVTKIDVLT
                     FHCGDTLIDFRDGRKYPTVQIGSQCWIAKNLDVGQQIVHGTKTQTNNDTIEKYCPYND
                     ADSCEKYGGLYTWDEMMEYTEIIGSRGICPCGWHVPSDQEWIDLEITLGMDQATANLY
                     NTWRGTDQGTKLKIGGSSGYEGLLSGCAIPGGYFSAVKMYEYMHSSTPYGIYGWRRCL
                     RTGDATVGRWNTFPKSYGLSVRCIKNQ"
BASE COUNT         5532 a         3882 c         3784 g         5320 t
ORIGIN      
        1 atcaggcagg gcgctcaccc tatacgaacc catgtcggca catgcacaag gttttctgtc
       61 accggaaaat cttgatatct atattaaatt atttgatagc gcactcatgg cttctgaagg
      121 aaacacagcg tattgccatc gcatacgaat ggcccggcag cctgtacttt atgcatggct
      181 cgaggtggcc aaatcgcttc cgttcactga taattggctg tttgaaaaag atgaaaaagg
      241 aatgcattcg gcacgtgaaa aagcaaggca atacctaagg gaattatgtg ataccgcaac
      301 cgtatacggc cccatgcttt ttcatgaaat aagcattaaa ccacaggaat attacgaacg
      361 gatgtcaacg tattttgaga acggtgttca gatgcacaag gctgttggga aaagcatatc
      421 gtttgccaca cccgcttctc caaagtatgc ggccaacggc ccaaactccc tgattgacgg
      481 ggtttgcggt accgacaatt atttttcctt atggcaggga tggtacggtg aagatgtgga
      541 cgccacgatt gatatgggtt ctgaagaatc cataagccgg attcaattga attgcctggt
      601 taatcaggca agctggatat ttgcccctgc aaacattacg gtgcgggcct ccaacaacgg
      661 caaggacttt attgaaatta tgacctttat aaatgccgac gcctctaaga agatggataa
      721 atcgattgtc ccatttctga ttgaatttcc tgtaccgcaa aactgccgtt acgtgaacat
      781 taaactgaat actatcggta accttccgga atggcacgga tctgaaggaa aaacctggat
      841 ttttgtggac gagatcatgg taaagtaatt aacagttttt cattaatatt gtatttatta
      901 ttgtccgata aaaattaaat ttgtaataaa ggatgtaaat aaaagttttc gttttttacc
      961 tcggtcctaa aggaagaatt ggttttcagc aatttacatc ctttaggata agaggtaaaa
     1021 taaattgctg gaaagcaatt cattctccgg tagaaaccta cccatattgt atgagcatat
     1081 atgcaaaacc caattccaat atggctttcc attaattttt taaattttta tcggacaaaa
     1141 gtgatattga ttataaaaaa atagaaacag atgccgtatt taaaacaatg cctttattgt
     1201 ttagccttat tgatcgtttg ttgtttaaca acaaatcccg tattttccca gtcaaaaatt
     1261 tccatcatcc caaaaccgca acaggtggtt ttgatgaaag attcgtttgc actgcatcag
     1321 gcgcgggtgg tcttgcccaa agaaacaaaa gcattaaggg tagcaacatt ttttacagtt
     1381 gctgttaaac agcaaaccgg gatcaatttg cttcagaaaa aaactctgaa taccatctgt
     1441 ttcagttatt caaaaaaaat aacacaagcc gaaggttata aaatcgatat ttcgtccaaa
     1501 aaaattaaca tcacggcaaa aaacgatacc ggcttattct gggctgtgca gactttaagg
     1561 caattgcttc ccgacagtta cggcaccgga gtttttattc cctgcatgca aataaccgat
     1621 gccccgcttt acggatggag aggaaatatg ctggacgttt gccggcattt tttcacggtt
     1681 gaatttatca aaaagcatat cgatatgctt tcgtattaca agatcaacac gtttcactgg
     1741 catttaaccg acgatcaggg ctggcgcatc gaaataaaaa aatatcccgg acttaccaaa
     1801 gtcggtgcct ggcgaaaaga atctcaagac tcggtttacg gtggatttta cacgcaggaa
     1861 caaataaaag aagttattga atatgcaggc cagcgaaaca ttacggtaat acccgaaatt
     1921 gaaatgcccg gacattgtct tgctgcgctg gcagcctatc cggaattatc gtgcaggaaa
     1981 attaagtttg aagtagcaaa ttactggggt gtaatgaatg atgtgtactg tgcaggaaac
     2041 gaaaaaacct accagtttct tgaagatgtg ctgaccgaag ttatggccct ttttccatcg
     2101 aaatacatac atattggcgg cgatgaagca cccaaatacc gctggcagca atgccccgat
     2161 tgtcagaaaa aaatacaaga tgaacattta aagaacgaag cagcattaca aagttatttt
     2221 ataaaaagaa ttcaacagtt tttgcaatca aaagggcgaa ttcttatagg ctgggacgaa
     2281 attctggaag gcggggcatc ccctgaggcc attattgaag tctggcgcgg agaagacaaa
     2341 gcaaaagaag ccattgacaa taaaaataaa atcattcaaa ccctgtacat cgatgcgcct
     2401 atgagttcat taactttgtc aaagaccttc catttcgatc cggctgtcaa agaaaataat
     2461 gaatatgttt taggcgccga atgcccgtta tgggccgaag ccgttaccga gttcaatact
     2521 gattacatga tgtatccccg gcttcaggct ttttcagaag ttttgtggag cggtaaaaca
     2581 agttacgatg atttcctgag ccggataaaa aaacactatt ccatgatgga taaacagggt
     2641 attttatatg gcaatgaaaa caagccaatg ctggaggtaa acttaaaata tcttccggaa
     2701 agcaaatgct ggaaaatata cgaaaagcat ggaaacggcg atatagtcat tcattacgat
     2761 tttaatggca aaactcccgg gaacccatcc ccggtttttt ccgattctgt aacgatacaa
     2821 aaacctcaaa ccatttcagt tattgcattt cataaaaacg ctccggcttc acttccggta
     2881 acaataaaaa ttgaagataa cagggcaacc ggcataaagc ctgtttataa aacggaatac
     2941 agtgaaaaat acaacgaagg cggtgtttac ggaatgacag acggcataac gggtacctta
     3001 aaatatggcg atggcacctg gttaggctgg gaaggcaaag acctagaaat ggaggtggat
     3061 ctgggtttag aaacaaatct gacctattta cagataaatt gtttgcagga agtacaatcc
     3121 tggatcatgt taccatccga cattgaattt tacatttcaa atgacaacat caactggcaa
     3181 aaactggcaa ccctctccca ctccgtttcc gatcaggatt taaaaccaca gatttataac
     3241 ttcatataca aggctcccca acccgtaaag gcccggtatg taaaagttat agcaaaaaac
     3301 tatggcaagc tgcctgtctg gcataacggt gccggaggta attcctgggt ttttgccgat
     3361 gaatttataa ttcgataaaa atataagatc gtcttcacaa ccacgctcct gcgttcgtca
     3421 tttagtcgaa agttgaaagt gaaaagttga aagtgaaaat ttagtacttt gtattgtgca
     3481 acttgcaact tccaacttgc taccttcaac tcgcgaataa ccgttcccct atttcagcca
     3541 ctggtccagc catccaaaaa actcccgctg ccacaggaca ctgttttgcg gtttggtaac
     3601 aaagtgtgtt tcgttaggga aataaagaaa acggctgggg atgccctgca attgcgcaac
     3661 attaaaggcc tgcatacctt ctgtataagg aatcctgaaa tcatagccgc catgaatgac
     3721 caaaatcggg gtatcccaat tgcccacgaa tttatgggga gaaaaatcat aactttgggg
     3781 tttgggatgt tcccagtatg cgccaccaaa atcgtggttc gggaaccaca tttcttcggt
     3841 agtgccatac caactttcga agttaaacat gccgcagtgg gcgataaagg ccttgaaacg
     3901 cttgttgtgg ttgccggcca gccaatacac cgaaaatccg ccataacttg cccccacggc
     3961 tcccaattta tctttgttca caaatggttc tttagccact gaatcaatag cagtgaggta
     4021 atcgagcatg ttttggccgc cataatccaa ggcgatctgg tcattccatt cttttccgaa
     4081 gccgggcaat ccgcggcggt taggagctac tacaatataa tcattggctg ccatcatctg
     4141 gaaattccac cggtacgaaa aaaactggct gacagtgctt tgcggtccgc cctgacagta
     4201 caacagggcg ggatattttt tgtttttatc aaaattgggt ggataaatta cccaaaccag
     4261 catttttttg ttgtcggagg tagtgaccca gcgttcttcc acattcccca tagtgatgga
     4321 agataaaagt tctttattag tgaaagtaag ctgtacgggc ttgtattttc ctttttcgtt
     4381 atcgatccga taaatttcag aaggcatgga catagacatt ttcactgcga cagtattttt
     4441 tccgtccgca gacaattcgc tgaaatcgtg cactccttcg gtcaactgat tgatttttcc
     4501 ggtggccata tcgcactgaa acacctggca ggtgcctttg gtatagctta agaaataaat
     4561 tgatttggca tcagcactcc agtttagtgt gctggcgctt tcatcaaaat tagcgctcat
     4621 atcggcaatt gtcttagcag agaaatcata gattatcagg cgttttttgt cggattcgta
     4681 gcccggagtt ttcatactgc accaggcaat tttttttcca tccggagaaa atacagggtc
     4741 cagatcatag ccacccatat ctttagagat gttttcggta agtcccgatt gcagatgata
     4801 aaggtaaata tccgaattag tactcaaggc ttcatctttt ccgtgcagtt tacggcaaac
     4861 gtacgccacc ttttgtccgt cgggactcca ggctatttgc tccatgccac cgtcgggagt
     4921 aagcggagcg tcaaattttt ctgttccaag aatatcggtt ccgccggtaa cggtaccgtc
     4981 tgcataagcg gaaactataa tatggctgta ggcatagtcg tgccaatcgt tccagtgccg
     5041 ataaaacaag tcgtcaatta ttcttgcatc cgcctgaggc aggtcgggat atacctcagt
     5101 aaccgtttta tccaatttaa tatcccgggt aaaaagtatg tgtttcatat cgggagcgta
     5161 agcaaaacct gttattccgc cttcaaatga ggtaatttgc tttttggacg ttccgtcagg
     5221 gttcatctcc caaagctgcg gcgtaccgta ttcggctgag atgtagccaa tttttttacc
     5281 atcgggacgc caaacaccat tccattcgct gcccggagaa ttggtaatat tgacggcagc
     5341 accgccggcc aggggaacca gatacaggtc gcggttgcct ttgttttgtt ccaggttata
     5401 aaaagtcaca ccatacaaaa tggatttttt atcaggagaa agttttatat cgcttacccg
     5461 accaaatttc cataatagtt cgggtgtgag cctgccctgg gcaaacaatg cggggataaa
     5521 tgcaacggcc agaagaaagg atactaaggt ttttttcatg gcaataaaaa ttttaatgct
     5581 aggttttttc gaatgattta tagaaaaaag aatattgcag acttcgctgc tttactcatt
     5641 taacaatttt attttcttga gatattatca aggcggttca taccttctat ggctttctgg
     5701 tagttcaccc tgatttccaa agctttttta taatcattgc gtgcggccat tacatttcct
     5761 gataactcat aagagtagcc gcggttataa taggcttcgg cataggtggg gtcgcacata
     5821 atagcatcgg aaaaatgttt tatggcaaca ttataaacct gcagatacac caggtgaatg
     5881 tacccgagat tataatgcgc gtatttgttt ttggggtcaa gttgcaatat cgtcgtatat
     5941 ttttctatcg ccttgttgta ttcattattt tcctgataaa ataaagccaa tgaatacagc
     6001 gcctcagtac tttgcgggtt gatattcagc gcattattga aataatctat tgccagcggg
     6061 ttatgtttaa cagagtacat caggcccagc tgcatatatg catggaaata ctcctgatct
     6121 ttttccacgg caatctgaaa gctcctgacg gcttttgcag tatcgcccaa atctttataa
     6181 gccataccct taataaagtc ggctttagca ttcagtttat caattttgag ggcattgtcg
     6241 gcaaattcaa aagttttttt atagtctttc tggtaataat aaagttcggc aagtttcaaa
     6301 acagcttcgg catcgtcggg attcatttcg attgccttat taagggcttc ctgacaattt
     6361 cttaatttcc cggtagcaaa ataaatatct gacaaggtac gcagatacag ggaatttttt
     6421 gagtctatag ttatggcttt attgatgtcg gtaagtgcct tgtcggtttc tttacggtca
     6481 agatacatta ttgcccgctg attatataat tccgcatttt ggggatctgc ttcaattttt
     6541 ttggtaattt ccgccagatt gacaggcaag gagtcggtat tagctatacc ttgttcggta
     6601 ctttgatgat taccacaggc aaaaagtatc aacagcgata ttattaccga taaataacaa
     6661 acttttttca tccaacaatt tttatttttt actgaattaa atgattatta tcagaaaaaa
     6721 aaccaaatta actaacccca tacccttgca tggggttaat taacaaagca tctttctttt
     6781 ttttgaggga aactggaaaa gcacaaatca gcttattttt tccctgattt tagactccag
     6841 ttcttcgcat agttcggggt tgtcgagcaa caattgtttc acggcatccc ttccttgtcc
     6901 cagtttagtt tcgttatagc tgaaccacga gccgcttttt ttgataactt ctttttccac
     6961 gccgaggtcc acaatttcgc ccatttttga aaatccttcg ccgtagataa tgtccacttc
     7021 ggcagttctg aacggaggcg ccactttgtt tttcaccact tttacccgca ccctgtttcc
     7081 ggtaacggcc tcgccatctt ttatttgtga tactttacga atatcaatac gtacagaagc
     7141 atagaacttc agggcatttc cacccgtggt agtttcgggg ttgccaaaca tcacgccgat
     7201 tttttctcgt aactggttga tgaagataca acagcaacgt gttttgctta tggtggcggt
     7261 aagtttgcgt agtgcctgcg acataagacg tgcctgcagt cccatttttg agtcgcccat
     7321 ttcgccttca atttcgcttt tgggtgtcaa tgcggccacg gagtcgatga caatgatatc
     7381 gatggcaccg gaacggataa ggttttcggt gatttcgagc gcctgttcgc cattatcggg
     7441 ctgtgaaata agcaggtttt cgatgtctac gcccagttta cgggcatacg aactgtcgaa
     7501 ggcatgttct gcatcgataa aagcagctat gccacctttt ttttgcgatt cggcaatggc
     7561 gtgcaaggcc aaggtcgttt ttcctgagga ttccggcccg taaatttcca ctaccctccc
     7621 tttgggaaaa ccacccacgc cgagtgcggc atcaataccc aaagaaccgg ttgaaatata
     7681 ttcgatgtct tcggctggat gatcgcccaa cttcatgatt gctcctttgc cataggattt
     7741 ttcaatttta tcaagggtaa gctgaagcac ctttagtttt tctttgttca gcgcattgtc
     7801 ttttttttct tcggtttctt tttcttttgc catagtagta tgtattaaat tatttgattt
     7861 tcaattcatt gagtatctga tctgtatgat tgccggtgtc caccttgtta aaaaccgctt
     7921 cgatggttcc ctgttccgat attacaaaag tggtgcggtt tatcccgtcg aaagttcgtc
     7981 ccatgaattt tttggggccc cacacgccat atagtttgca gattttttta tccgtatcgg
     8041 caataaggga gaaaggcaat tgaaattttg taataaactt tgtatgagac ttttcatcgt
     8101 cggcactgat gccgagaata gcaaaacctt tgtccaaaag aagctgataa ttatcgcgca
     8161 ggttgcatgc ttcagccgta caaccgggag tattatccct ggggtaaaaa taaagcacta
     8221 atttttttcc tgagaaatcc ttaaggctaa cttccttacc atcttgattt tttccggtaa
     8281 aatcgggggc tttatcccct ttttttaagg tatccatagc taaaatattc tcatacaaaa
     8341 taaagaattt ttatcgtaag atttgatgtt agttgtaaaa aaaaacaaaa aaacttatat
     8401 gattgctttt cagtattata aaaactcatc ataaaaaaag ctgcgggttg taaaggcaaa
     8461 gggatggtca attatggctg ccgcattaaa aaaaacagta actttgcttt ttctcaagaa
     8521 caaatgcact tatgactata gaaaacaaat cattaatgcc agattacctt tttgaagtaa
     8581 gctgggaagt atgcaacaag gttggcggca tatatactgt gattgccagt aaagcgccgg
     8641 ggttgacaaa aaaattaaaa aacaactatc tcctgatcgg gccggatgtc tggaaagaaa
     8701 caccggtaaa tcctgatttt attgaggaca aattccttta caaatcatgg agagaagatt
     8761 ctgaagctaa agggttgaga ttcaggatag gaaattggaa catccctgga agaccggttg
     8821 ttatcctggt ggactttacc caatattttg cccaaaagga cgatatttta tcacggctat
     8881 gggaaaaata tcaggtagat tcctacaccg gccaatggga ttatattgaa ccagcactct
     8941 ttggcattgc ggcttcacgg gttatacaaa gtttttacga gtttcatgta attgccagcg
     9001 accatattgt ggcacagttt catgaatgga tgaccggctc cgggatacta tcgctgaaag
     9061 gcagggtgcc ccagattgcc acagtattta ccacgcacgc taccatactg ggaagaagca
     9121 tagccggtaa cgggttgccc ttatatagta atcttaccga atatgataat gatgtacagg
     9181 cacaaaaaat gaacatcatg gcaaaatttt cgcttgaaaa aaattcagca aaattttgcg
     9241 actgtttcac aacagtaagt gaaattactg cccgggaatg cgctcatttt cttaaaaaag
     9301 aagttgatgt gctaacccct aatggttttg accattcact cgtccccgaa aattcggatt
     9361 ttgaccaaaa gcgaaccacg gccagagaga cgttgaaaaa agttaccgaa ggtcttatta
     9421 atcagcccat tgatgacaat gcggtgttcc tgctcaatag cggacgttac gagtttcaca
     9481 acaagggtat cgaccttttc atcgatgcac tgggaaaact taaccgcgag gggaaccttc
     9541 cccatactgt agttgcctgt ttgtcgatac ccgcttatca tgctggcccc tcccgcgagc
     9601 tgagccagcg tatggaaaaa cgtgatttcg gtcaggcagt ttcttatgat tactgcactc
     9661 actacctgca caattacgac aaggatgcca tgatcagccg cctcaaggcc aaccacctga
     9721 acaactctcc ttcagataag gtaaaggttg tcatcatccc atgttatctc gatggcaaag
     9781 acggcatcgt caacctgaat tattacgatt ttctgatagg ctttgatctg actgttttcc
     9841 cctcttatta cgaaccatgg ggttatacac cgttggaaag tattgcattc cacgtaccta
     9901 ctatcactac ttcgctggct ggatttggcc tttgggcccg tagccaggga taaggaagag
     9961 aaaacgcggt gacagtaatt gaacgggatg ataacaacgc cgagttcgtt accggaatgg
    10021 ttgccaaaaa aattgttgat tttgtcaata gtacagcaaa agaaaaagat accgcaagac
    10081 aaaacgctta cgaactgtca aaaatggccc tgtgggatga actactcagc aactactatc
    10141 aggcgtacca taatgcactt gaaaaagccg gcaaccgcta tgaagaatac aaatcaaaat
    10201 cattaccggt acaatatgag attataccac aaaaacaact taatcccaaa tggaacaagg
    10261 tacttgtaag gcccagcatc atcgaaaaac tatcgccttt gtacaaactc acacaaaatc
    10321 tttggtggac atggaacctt gaagctatcg agcttttcga gatgataaat ccggagttgt
    10381 gggaacaatg taaacaaaat cctatgacgg ttcttgaatc gctttcgctg gaagattaca
    10441 aaatgctggt aaaagacact gtttttcgcg aaaagctgga caaagtattt aagaagttta
    10501 ctgattattt agcatatggt cccaaaaaag ccaaagaaca ggtggcctat ttcagcatgg
    10561 aatttggcct gcatcactct ttaaagacat attccggcgg actgggcatt ctggccggag
    10621 attacctgaa agaagccagc gactccaacg ccaacatcat tggagtaagt ttattgtacc
    10681 gctatggata tttcgatcag agcatctcgt tgcttggcga tcaggtacct tcctttcatc
    10741 cccagaaata ttcgcatctg ccggtgatac ctgtccgcga caagaaactc ggagccgatg
    10801 ggagcagcac ctgggtaaag attcagatag cagcgccggg tagaataata actgcaaaag
    10861 tatggagagt agatgtcggc agggctgcac tctacctgtt agatactgat attgaagaaa
    10921 atacagccga agacaggact attacacatt gcttgtacgg cgatggttcg gagttgcgat
    10981 ttaaacagga attgctgctt ggcatcgggg gtatccgtct gctggagcgg ctaaaaatat
    11041 cgcctgatgt ttatcatatc aacgaggggc atgccgcttt cagtgttatt gaaaggcttc
    11101 gcaaatgtgt acaggaagaa aacctgactt atcaagaggc gctggaagta gtaaaagcct
    11161 ccacactttt caccacacac actccggtgc ctgccggaca cgaccgtttc agcgaagaca
    11221 tcctgagggc ctaccttcat cactatgccg acatgctcaa gatcagctgg gacaccttta
    11281 tgaatcttgg gaaagcccgg gaatttgacc cgaccgaaga attcagcatg agctatctgg
    11341 cagctaccct atcgtcagaa accaacggcg tgagtaggat acacggaagt gttacccgcg
    11401 acatgtttgc agaactgtat ccgggatatt ttcccgaaga attgcatatc agccatgtaa
    11461 ccaacggcgt ccatttcccc acctgggtat caaaacgctg gaaaaaactc tacgactcgg
    11521 tgtttgatcc ggaatatatt aacgatcttt cgaatcatga atactggaaa aaaatatacg
    11581 gtgtacctga tgaaaccatt tggaaaatac gacaggaaca aagatccgaa ctgatcaatt
    11641 ttttaaggag ccgcctgatt aatgaaatga ccttgcgcca ggataatcca cagaatattt
    11701 tcaatgtttc gcaatcgctt aatccaaaag cacttactat tggttttgcc cgccggtttg
    11761 ccacctataa aagagccaat atgctttttt cgaatttaga ccgcctatct aaaattgtaa
    11821 acaatcctga ataccctgtg caatttgttt atgcaggcaa agcgcaccca aaagatgttg
    11881 aaggtcagaa acttataaaa aagatatatg aaatttccat gatgacggaa tttcttggaa
    11941 aaatagttat cgtagaaaat tatgatatcg aattaggcaa aaaacttgtc cagggagtag
    12001 atatttggct taacactcct acccggccac aggaagcctc gggaacgagc ggcgaaaaag
    12061 cggtcatgaa cggcgttata aatttcagtg tattggatgg ttggtgggct gaaggttatc
    12121 gtccggaagc tggatgggcg ctgaaagaag aacgcaccta taaaaaccag gcagcccagg
    12181 acgccctcga tgcagctaca atttacaata tgcttgaaaa tgaaatagcg cccatgtttt
    12241 ataaacgcac caaaaaagat atacctttgg aatgggtgca gaaaatcaaa cacaccatcg
    12301 ccctgatagt ccctgaatat actacgaaaa gaatgttaga tgattaccgg gataagtatt
    12361 acaggaatat ttgcaaacgt tccatagaca tcaattccga caattatctg aatgctaaaa
    12421 agttagcagc ttggaaacaa aaggttttca gagcctggga tagtattgaa atagtttcgg
    12481 ttaccatccc tgattcgaat aaaaaaccgt taagtctcgg tgaaaagttt tatgcggaga
    12541 taaaaataaa acttcacgaa ctaaacaaaa atgatgtcgg tgtggaaatg gtttttggtc
    12601 agaagtccga cgatgtggtg aacaggatat tattccaacg cgagctcaag attaaaaata
    12661 ccaatcataa tgtgattact tactcaatag aatttccatc tgtaaggata ggtgtatttg
    12721 attacgcttt cagaatattt ccaaaaaatc cattgcttgc caacagacag gactttagtt
    12781 tgatgaagtg gatatagagg ggaaagttga aagttgaaag ttgaaagttg aaagtggaaa
    12841 gtggaaagtg gaaagtacca agtagcaagt agcaagtgga aagtttaaag tgaaaagttg
    12901 aaagtggaaa gtagtcaata gccaatagtc attggtcaat agtcattggt cactgttcat
    12961 tgttcattga taattgctaa ttgctaatgc ttagtctttg attatcagtt ttccgcttcc
    13021 aatgatgttc ttagtatcgg taagtgaata aaaatacatg ccggccaata gactgcccct
    13081 gttaatggta ttttttccgt tttgaatgtc gggaatgtac atcacctcgc gacccatcat
    13141 atcatatatt cgtaattcat aatgagcgtt gtcactaaaa tttggcaatt ccagtgtggt
    13201 tgagttgata aacgggttgg gataaatatt ataatccttt ttttcgtaat tattaaccgc
    13261 caccgtaagg caatttacta cagagggcag gccgatactg acaaaattcg agtcaacagt
    13321 gataatgcgg taagcacctt caaactcctg tgcagtttga caataacgtg tgctggcggt
    13381 ccagttttcg ccaaccacat taccctgacg gtcggctacc acaaattgat gcacatggcc
    13441 agccaacacc acatccacat tattagaatc gcaaatattc ataaaggttt cacggttata
    13501 caggaatgag ccatcgtcgg gccctgcaat agttccggta ttgggggagc catcaatttc
    13561 ggttccattg acattaactg gcgggtggtg catgacaatt attttccttt tatttcctgc
    13621 caagctcaat gtacttcgca gccactgaca ttgtgcagca gcaatacctt cgctttcgac
    13681 acaagccgga tgcaaatcct gccaaatcgg ccggtcggcg ccactcctga taaataccat
    13741 aaaagcatca ttgtacgtaa aataataatc catatcctgg cctaggtata atggataaaa
    13801 caacagatta tcattaatta ccggtggtac aatagtttta tagtattcat ggttgccgga
    13861 aacaaaatat atgggaatag tcattgcaga atcgacaaaa tatgcgccgt ttgcaggatt
    13921 agttataccg ccctgagggt aaagatgctg agttagagca tcatacattc catcaggatg
    13981 gatggagcct acattggaaa tatctccgga ggctaccaca aaggccggct tgggattcag
    14041 ataattaatt tcctttaagg tgcattgaaa ttgttcaccg tcggaatcgt tatttcccac
    14101 gctacccccc aaagtcccat cggagacatg tatatcagaa atatgaacaa aagaaaactg
    14161 ctgagcaaaa tcaattttac aaaaaaacat cagcgaaaaa taaagtaaaa aagtcaggag
    14221 taagtttttc tttttcataa aatagtatga taaaaaaatt ctgaaataat ttttacaaag
    14281 gtatgaaaaa aaaggagtaa caggaacttt tcgggcacaa aatgtattcg aaattgttta
    14341 aaaattgaag atgaattttc aggttatagc agctagccct ggtcagtatt caattccttt
    14401 tctggccttg acccctcgtt ggtaatagtg cttcacttct ttcatactgg ttaccaggtc
    14461 ggcgaattta atgatttcat ccgcagcata cctaccggta agtattactt ccacatgatt
    14521 ttttttgcat tcaaagcatc gagcagttga tggtttgata gcaatcccag atggatggcg
    14581 ataaatattt cgtcaagaat aatcacatcg tagatgccag acgccatcat ttttttactg
    14641 tttgcaggac tttttctgcc gccgctatat ctttggccga tggctccctg ataagaagac
    14701 aacccaaccc gaatttttta atggtgatct ggggtaaaaa tgtccggatg catagcattt
    14761 cattataaga ttttgccttg acaaattgag caaaaaaact tttttaccgg ctccggccgc
    14821 tctcaaagcc aaacccaggg ctgctgtggt ttttcctttg ccgttccctg tataaatatg
    14881 aatatatcct tcatcatcta acgggttatg tatatttgat tcttgttgca agccggtcat
    14941 tttacctggc cataattaac ttcctaatca attgattttc ccctgaaata atccggacaa
    15001 aatataatcc attgggacaa caacccagat cgaaggtacg ttggctgctc gagaggccat
    15061 cagttaagac ttccacgccc tgtgaattat atatttttac cgttgcgccg gaaatatttt
    15121 taatgataag taactctttg caaggatttg ggaataagac tattttttca tcaattaata
    15181 agggttcctc tacctttgtt gaagtatttt caacgccttc aaatacgcag cgcaaatgat
    15241 tggttgtata aagaaatcct ccgcagtttt cccaatatcc atctgtaatc ctggtagccc
    15301 atctttttac ggtataaatt ttatagagcg acgatgtcca gatataatag ggagaaatgc
    15361 ccatataggc actgtcgagg ccccatccgg ttccttcatt atcgttaaaa tgccccacct
    15421 ccatatctgt cggcaaacgc caaccctcgc catatttatt gacacaataa ctcctattgg
    15481 cagtggtcag cgtttgctgt tgagaaacct tttgcatggt accggtattt ttaagatcac
    15541 cggtattaat caactcctcc ccgtcccaaa tatcataaat tccactgtcg ggtaaaaatg
    15601 ttggataagc caggcctgaa accgggccat cgatagtggc aaaacagtca tcgtaattgc
    15661 agtatggact attgtattgc agggcagccg cataggtcat gcctacatcg gaaacagggc
    15721 taacaaaaag tttgcttctg ttaaaatcat ttggggttag tatagtctga ttgtcccagt
    15781 tgaatgccgt atcgctgata ttgccgccga caccataacc gaaaaaggca ttaacatgta
    15841 ctctttttcc atcaggaata cttgtcttat ccggtatcag aatgtgatcg ccgcaacgat
    15901 acactttatt aaaagtgtca tagttgtaca ctgcgcttcc gtttttaatg gtaaccggat
    15961 taaacccaag ccatttttca tttatatatg cctcattgcc atggttgtaa ttgctttgac
    16021 caacagccat acctacggaa aaaatcatcc agaaaaaggc cggaaatttt ttcataattg
    16081 tgttttcttt cagtttttct tttttacttc gcctttgatg gtaagtgtta ctgttctgcg
    16141 gacattgtcg ttagagttga ctgtgatagt tttactgaaa acccccaccg tattggtgtt
    16201 atattttaca tttacgctgc ccttagtccc gggtttcacc ggctccttgg tccaggtagg
    16261 tacagtacat ccgcaggacg cttttacctc ggtaataatt aggggtagtt tcccggaatt
    16321 ttggaaagta aaaacgcatt cggccttttc gcctttattg ataatgccaa aatcataaac
    16381 caacgtttca aattctatga ctggcttccc ggtttcttgc gaaaacaagt agcaagaaaa
    16441 agtcattaac aacaaaacac acaaaatttt tttcatatca ttccaatttt tgtttttttt
    16501 caaatgaaaa atctctgaaa acactgagtc caaccgaaca ggtattggcc agctgaattt
    16561 tattataata ttgataaacc ggaatatctg ccataacaga aacattccag ttatcattga
    16621 tagaaacatt gacttgtggt acaacaaaaa gcaaaacact gcccgaggca tcaacgggtt
    16681 gcctgaagtg tttgtttttt tcttttatct ggtttttgag ttggaaaata aatgtccagt
    16741 cttttaattt tagtttttcg aaaacaaagt gttttgaaaa aaagaaagaa gtagaataca
    16801 ttgttccaaa aaggtattct tgtttgttaa taaaatactt ttcaatacgg ttggtcaaaa
    16861 aaaaccggat accctcaaaa gaatattcct tgataataaa tgtctgaaaa accattccaa
    16921 acgatcccgt tgaggcctgc aaatcaacgg gcagtgtaac cccatccacg gcttgaaaat
    16981 tatgggtaaa agggatattg gcacccaggg cacaagaaat ttcaaagcgt ttatccgcat
    17041 tataataaag tctgggtttc agagaaacca ctgcattgga aagtccatac ccctttaatt
    17101 tcaaattatt aagcttataa acttgggttt tattgatata ataaccactt tcggcttcga
    17161 cagtaagttg atccagaata ccataaccta taagcaagcc caggtagtta aaatttgcat
    17221 ctttaagaat tcctgcgcta cctgtatatt ttttgtcgcc agtaaggtac ctgtgcgaat
    17281 aagaaaaacg gtaaaacata ccaagccgaa aagttttttt gtccatcact cccaggttcg
    17341 cagtaccgcc cacagggttt ccggcagagg caaaacactg cgcccaggca ccttgcgcac
    17401 acaacatcca caaaaaacca aatgccagtg tcctgccaat cataatactg taatcgtaat
    17461 tgtttttgtt tccgatttat tggaggcctc aacagtgcac gacacctgat tatttcccgg
    17521 agtacaggta ggcgcaacat aatcaaccac atttcctgac cccaaaatat ctccagcagt
    17581 agccgaccac agaaatgtaa ggttttcgcc atccacgacg gccgtaattt ttgtggaatg
    17641 acctgtaatg atagtatcat tttcggctgt caggctaata aaagagttta ataaactgcc
    17701 atcattttcc tctttacatg cagaaaacat acaagccaat gcaacaaaaa gaataattaa
    17761 ttttttcatt ctgattcagt tattgatttt ttatacacct tactgatagt ccataacttt
    17821 ttgggaaagt attccatcgt cctacagtag catctccggt tcgcaggcac cttcgccaac
    17881 catagatacc atagggagtt gatgaatgca tgtattcata cattttaaca gcgctgaaat
    17941 acccgcctgg aattgcacaa cccgataaca gcccttcgta tcccgaagag ccacctattt
    18001 tgagcttagt tccctggtcg gtacctcgcc aggtattata gaggtttgcc gtggcctgat
    18061 ccataccgag tgttatttca aggtctatcc attcctggtc gctaggcaca tgccacccac
    18121 atgggcaaat acccctgctg cctattattt cagtatattc catcatttcg tcccaagtgt
    18181 acaatccgcc atatttttcg cacgaatcgg catcattata cggacaatat ttttcaatag
    18241 tgtcattatt ggtttgtgtt tttgtgccat gaacaatctg ctgcccgaca tccaggtttt
    18301 ttgctatcca gcattgactt ccaatctgca ccgtgggata ttttcgccca tccctgaaat
    18361 caatcagtgt atcaccacaa tgaaatgtga gaacatcaat ttttgtaaca tcagagtata
    18421 caggcaggca ggttccactg tttaccttag cccggtaaaa ataagattgt gaaggaacta
    18481 aagggaaatt attgcttgtg gctccggtaa tatcagtc
//
LOCUS       DAOSFP010000005        13898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_3654_length_13898_cov_3.940620, whole genome shotgun sequence.
ACCESSION   DAOSFP010000005 DAOSFP010000000
VERSION     DAOSFP010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13898
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3654_length_13898_cov_3.940620"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..803)
                     /locus_tag="PLI16_00390"
     CDS             complement(198..803)
                     /locus_tag="PLI16_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83045.1"
                     /translation="MKKISVNVILLLVLIFLSTNYRAHAQEPADTSDATLEETMSWIQ
                     SKVNEKMYKISYEYVSCADKLSKTVSATSVNCHIRFEYDISKLTFSVTSTFATIWTTK
                     DSIVQPSAFTLYLSDISAINLVCYKDTVYKETREYENTDQKGNEIQTCEVTNTMYSYC
                     SMFFTANGKKYEMLIPDEFIYIRLLKAFNHAVGLVKKTEKF"
     gene            complement(1137..3164)
                     /gene="uvrB"
                     /locus_tag="PLI16_00395"
     CDS             complement(1137..3164)
                     /gene="uvrB"
                     /locus_tag="PLI16_00395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOH83046.1"
                     /translation="MKFELTSDYVPTGDQPEAIRQLAEGLQRGDEAQVLLGVTGSGKT
                     FTIANVIQEVQRPTLVLSHNKTLAAQLYSEFRQFFPKNAVEYFISYYDYYQPEAYLPV
                     TNTYIEKDLSINDEIEKLRLSATSALLSGRRDVIVVSSVSCIYGIGNPEDFANSIIRM
                     KIGDTVPVKKFLQALVNILYSRNDVTFGRGNFRVRGDTIDVSPAYADFCIRVCFFGDE
                     IESIETFEPVNGRFIEKLDAISIYPANLFISSQDKMKKAITDIHHDLISHIAYLKDMG
                     KMMEAKRIEDRVSYDLEMMRELGYCSGIENYSRYFDGRKPGGRPFCLLDCFPDDYLMV
                     VDESHVTVPQIRAMYGGDRSRKQTLVEYGFRLPSALDNRPLKFDEFEGMLNQVVFVSA
                     TPADYELQKCEGVVVEQVLRPTGLLEPVIEVKPSQTQIDDLLHEIDLRVKVNERVLVT
                     TLTKKMAEELTTYLLKLEIRSKYIHSEVDTLERVQILRELRAGDIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLTQTAGRAARNINSKVIMYADKITESMQRTIDET
                     DRRREKQLKYNAEHNITPTQIIKSVDATFGQTSVTDSKGNEVKAYVDNTNSSVAADPV
                     VQYMKKEQLQKLLSQVKSDMAKAVKALDFMEAARLRDEMYELEKILKDKSK"
     gene            complement(3169..4311)
                     /locus_tag="PLI16_00400"
     CDS             complement(3169..4311)
                     /locus_tag="PLI16_00400"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOH83047.1"
                     /translation="MAKKSTKKLHFFWKLLLVANILAALLLLVSFSSSFINPRVFWLP
                     AFAGIIYPYVLIVNIAFIVMWLFIRLKYSLISLLPVLLGFSYFSSFLQCNSEKPANGN
                     GLAFKVMSYNVHNFDLYNYKKNWQINYEKRNKIFSFLKNQDCDIICFQEFVNNCNGEF
                     KTKDTLVTFLKATNVHAEYSVVSRHIYEFGLATFTTFPIVGKGLIKFPNSRNNFCIYT
                     DVLISNDTVRIYNAHLQSLHLGNSDIEFADNIASGNTNTDEKDLKNKSLRILRYLKRG
                     FVKRSVQAELLAGHIKKCPYPVILCTDLNDTPFSYAYHQLGSLLNDAFKEAGFGLGTT
                     YYKIYPSFRIDYIFLSNHFKVSNFKNVKTDNSDHYPVMCLAEKKKQ"
     gene            complement(4315..5286)
                     /locus_tag="PLI16_00405"
     CDS             complement(4315..5286)
                     /locus_tag="PLI16_00405"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH83048.1"
                     /translation="MTGNNYYNFNRNFSGQPYGQRRPSMLDGMKRFFTGKSTLSILII
                     INIAVFLIINLSRLIFKLYLVSPYQFHEGSITVPGYWLAVPADLELLILKPWTIITYM
                     FTHESIWHLFFNMLMLYVGGVIFTEYLSSKRLLLVYILGGITGALFFIISYNVFPAFA
                     QDLNIPALGASASVMAIIIAIAAYVPDYPIRLFFLGAVKFKWVALAFVIYDLWTLIPG
                     QSFNNNPGGHIAHLGGAFFGFFYILALKKGLVVKQLTNPFKKWFVRKPKVKNTYTRKP
                     KTDDEYRYERKAHQQRIDNILDKIKKSGYESLSKEDKEYLFKESKNK"
     gene            complement(5273..6127)
                     /locus_tag="PLI16_00410"
     CDS             complement(5273..6127)
                     /locus_tag="PLI16_00410"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH83049.1"
                     /translation="MGMEQYSPTGFRMLPTVVKNLLIINGIFFLATTGLGQAFGIDLY
                     DKLGLHYVASEKFSPYQFITYMFMHGNFMHLFFNMFALWMFGYVLENFWGPKRFLIFY
                     LVCGLGAAVTHYAIVFFELRPVIHALDVYLTDPGHAQLSQLLNSGYVDGGYPPLKNFI
                     TQYNLLINNDMSKAITYSAEFVMQYKMDLLNAPVVVGASGAVFGILLAFGMMFPNTRI
                     YVYFAIPIKAKWFVLIYGALELVSGISNVQGDNVAHFAHLGGMLFGLAMILFWRWQYK
                     KRQFHDRQ"
     gene            complement(6290..8077)
                     /locus_tag="PLI16_00415"
     CDS             complement(6290..8077)
                     /locus_tag="PLI16_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOH83050.1"
                     /translation="MNDQNYLKGGEFITKAAAEIFIPEEFNEEQKMIADTCDDFLEKE
                     VIPILDEIDTKKEGLMRSLLSKAGELGLLGISIPEEYMGFGQSFITSMLTSDRLGAGF
                     SFAVAYSAHTGIGSLPILYYGNEEQKQKYLPKLASGEFAGAYCLTEPNAGSDANSGKT
                     RAVLSADGTHYKLNGQKMWITNGGFADVMTVFAKIDDDRILSAFIVERTFPGISFGAE
                     ENKMGIKGSSTVQIFFNDCEVPVGNLLGKRGEGFRIALNILHIGRIKLGANVLGACRY
                     TINKSVNYANERKQFGSKIASFGAIQYKLAEQVIRLFAVESAVYRASKYVDDTISIHV
                     AEGKSKAQAYLEAIGEYAMEAAILKVFASESQDYIVDEAVQIHGGMGFSAEMPVDRCY
                     RDSRINRIFEGTNEINRMVCADMMLKQAKKADSRIFAEASALYEGMETLRKAWQHEAP
                     ETSYFDYSGKVIENFKKTILLIMGRAYETLGKQYSSEQEVQMNISDMLIQTFAAESLM
                     LRVKKLEQIKGEEQVKIYKDIMDAFFYEAAARIRKSATDAICAFAVGDEFCKLKNALE
                     TLTAVKPLNIKDARRRVAAKLIDENLYGF"
     gene            8235..9026
                     /locus_tag="PLI16_00420"
     CDS             8235..9026
                     /locus_tag="PLI16_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83051.1"
                     /translation="MRRLFTISFLLLFTVFAGISQNTPKTDSVTNHWGISLYGSADYN
                     MYMVQGTTPAGQDAGFMKNIDLPRIGYTIGASVNRPVGKRVSMEFGVQLQSQGYRSKN
                     LIDTLWNYRDSAIGFSDYEKAYRYFSVAIPVMVKIKLFDMGKAHLDLGLGVAPVLSAG
                     KQQALFFDDHTEILNEKSGTAINVQALGCLSFYIPMGNKMSLTLEPTFRYNILPYKDS
                     YYIGVKRNLFSAGLGIYITRKVTDDEMYDYFYRRIYKVKNLPPAF"
     gene            9050..9493
                     /gene="smpB"
                     /locus_tag="PLI16_00425"
     CDS             9050..9493
                     /gene="smpB"
                     /locus_tag="PLI16_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOH83052.1"
                     /translation="MLSKVQIKNRRASFEYFIIEELTAGIVLTGTEIKSLRDGKASIN
                     EAYCVFKDNELFVINMHITEYSYGTYLNHLPKRERKLLLNRRELKKWLGKVKEKGFTI
                     VPTMLHINDKGLAKLNIALARGKHSYDKRETLKEKDIKREMDRKE"
     gene            complement(9590..10252)
                     /locus_tag="PLI16_00430"
     CDS             complement(9590..10252)
                     /locus_tag="PLI16_00430"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOH83053.1"
                     /translation="MHKEYLKMLASMMFPFAVVTVMWLVKIIEFYTKSDFSDFGIMPL
                     TGAGLIGVVTAPFIHADFGHLIANTIPLFITLSLIFYFYNEVAYQVFLLVWLFTGVWV
                     WSFARGDVYHIGASGLVYGYVSFLLFSGIIRRNARLMAISLLIVFLYGGFFWGMFPDF
                     FPKRNISWESHLMGGLAGLVLAVYYRKKGIQRDVYRWDEDDEEDEEDDFLQMDDEHSI
                     KH"
     gene            10409..12028
                     /locus_tag="PLI16_00435"
     CDS             10409..12028
                     /locus_tag="PLI16_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH83054.1"
                     /translation="MKNHFRTILFRPLFLFVLCFTFYANNGYAQQHDPKKTENKFAAA
                     LQIIRLQYVDTTNEEKLTEVAIRSMLKELDPHSVYMSKQEIDKANEPLLGNFEGIGVQ
                     FNIIKDTIIVITPVPNGPSEKLGIMSGDKIVKIDGEDATGEKINSDFVVKRLRGPKGS
                     SVKVSIFRRGKKELLDYTIVRDKIPINSLDAAYMSTEDIGYIKLNKFAQTTMSEFSEA
                     LSKLKKQGMKNLILDLRDNNGGYLNTAIELADEFLKKGQLIVYTEGVHSNRSVNLATE
                     KGNFEKGKLVVLINEGSASASEIVTGAVQDWDRGLVIGRRSFGKGLVQRPFTLPDLSV
                     IRLTTAHYYTPTGRCVQKPYNDGIEKYYKDLTNRYKHGELTNPDSIKFPDSLKYYTPN
                     NRLVYGGGGIMPDIFIPLDTTKVSDFYQDLLRKNIINLYVLKYLDINRETLKKQYPDM
                     EVFLKGYNLTNDFMQDFLKYSEEEGVKKDEEGYKVSETYIKNFIKAWIARNLWDYDAY
                     WRISNEQDDVYLKAVEAIQSKDMFKNYKIFY"
     gene            complement(12089..12712)
                     /locus_tag="PLI16_00440"
     CDS             complement(12089..12712)
                     /locus_tag="PLI16_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83055.1"
                     /translation="MEKIKYLLLLSFLWISCNPLFADSPLTSTDFYKAYQHEKMVVRA
                     AATDGVLTNQLIKFLLAKKKPIAVKMAVINRLGWSLDGKQNASVFFDYLQEHKGYKGT
                     DDLLKRAGASELLCMAYLKAMDNYFEVDEAIVYAEKAVSKNPKSFTYNIICALIKAQK
                     AMDGDWCMVFKITDRVRKDKSLQMDMKQEAFNIIFEYMDLYQDDCDD"
     gene            complement(12816..13106)
                     /locus_tag="PLI16_00445"
     CDS             complement(12816..13106)
                     /locus_tag="PLI16_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83056.1"
                     /translation="MAVSHHSGTVTKNTSGDELTAVHKYETGKPRKQKSKLNRAKKTK
                     ALSYSNHAVKKGRWQNTKKINCSVNPGLKAKRNWKKESPPPVNERKKTVKVY"
     gene            complement(13373..>13898)
                     /locus_tag="PLI16_00450"
     CDS             complement(13373..>13898)
                     /locus_tag="PLI16_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HOH83057.1"
                     /translation="EKIRLLVHRWRSEEQAILAGKNTVLKDDPQLTVREWYGKNPLRL
                     VIDRQLAISTSTKIMDNAAQTVVFNSVKEAVCGNIEYKKLDFSKNIIPEMLTVLYEKN
                     IQSLIVEGGCVTLQSFIDEGCWDEARVFSGNCFFFNGIRGPVINGRLENIQNIGADKL
                     STITNNRADSSWCS"
BASE COUNT         4147 a         3056 c         2848 g         3847 t
ORIGIN      
        1 attttaacca cctgcctgcc taccggccag gcaggcataa ccctgtcaga ctgtcgcaga
       61 caggcaggca aattagaaat atcaatacgt cttattggac taatggatat tctattttgg
      121 caaactggtt tttggagagc gtaccagcct gcccggggtt gtgtgataaa caagacatgg
      181 ttaaacgaaa cagcaaatca gaatttttcg gtttttttaa ccaggccgac agcatgatta
      241 aaggccttta acaggcgaat gtaaataaat tcatcgggga tcagcatctc atattttttc
      301 ccgttggcag taaaaaacat gctgcaatag ctgtacattg tgttggttac ctcgcacgtt
      361 tgtatttcgt tccctttctg gtcggtattt tcatactcac gggtttcttt gtacacagtg
      421 tctttgtaac aaacaaggtt gatggcgctt atatccgata aataaagagt aaatgccgaa
      481 ggctggacta tggaatcctt tgtggtccat attgttgcga aagtggaagt aaccgaaaaa
      541 gtcagtttac tgatatcata ttcaaaacgg atatggcagt taacggaggt ggcgctgaca
      601 gtttttgaca acttatccgc acaagaaaca tattcgtaac ttattttata cattttttca
      661 ttcaccttgc tttgaatcca actcatagtt tcttccagcg tagcatcgct ggtgtcggcg
      721 ggttcctgtg catgagccct gtagttagta cttaaaaaaa tcaaaaccag cagcaggatg
      781 acgtttaccg atattttttt catatttacc tattttgatt gtccttcaac agcactttat
      841 ctgaagctgt atttttagtt aagttcaaca gaaaaaacaa caaaaaatat tcaaaaaaaa
      901 tatttatacc gattgcacat ttgtaatagt cccaggaaat ggcctataac aaccgttgca
      961 tcggcattat accataaagc ccgtacatat ttataatttt taattggtat tatatctgtg
     1021 acaaatataa tccttctcag aacattatgc aagaaataac aaagtatttt tccctggcaa
     1081 caattgcggt aaacaattta ccataaaaaa cccaggcgcg cactaatttc aatctactat
     1141 ttggatttat ctttcagtat tttttccaac tcatacatct catcccgtaa ccgggcggct
     1201 tccatgaaat ctagggcctt aacagctttt gccatatcgg atttcacctg cgacagcaat
     1261 ttctgcaact gctctttttt catatactga accaccgggt cggcagcaac actggagttt
     1321 gtattgtcca cataagcctt tacctcattg cctttgctgt cggtgaccga ggtttgtcca
     1381 aaagttgcat ccacgctttt aatgatctgg gttggcgtaa tgttatgttc ggcgttgtat
     1441 ttcagttgtt tttcacggcg tcggtcggtt tcgtcgatgg tgcgctgcat agattcggta
     1501 attttatcag catacatgat gaccttgctg ttgatgtttc ttgccgccct tccggctgtc
     1561 tgggtgagtg accgttcgga ccgcagaaaa ccttctttgt cggcatcgag tatggctacc
     1621 agcgacacct caggcaggtc aaggccttca cgcaggaggt tcacccctac cagcacgtca
     1681 atatcgccgg cccttaattc gcgcagaatt tgcacccgtt cgagggtatc cacttccgaa
     1741 tgtatgtatt tggaacggat ttcgagtttc aacaaatagg tggtaagttc ttcggccatt
     1801 tttttggtga gtgtggttac caaaacccgt tcattgactt ttaccctcaa atcaatttca
     1861 tggagcaaat catcaatttg cgtctggctg ggttttacct caattaccgg ttcgagcagc
     1921 ccggtaggcc tgagtacctg ttccaccaca acgccttcgc atttttgcaa ttcgtaatca
     1981 gccggagtag cgcttacaaa aacaacctga ttgagcatac cttcaaattc gtcgaatttc
     2041 agggggcggt tatccagagc cgatggcagc cggaagccgt attccaccag ggtttgtttg
     2101 cgcgaccggt cgccgccgta catggcacgg atctgtggca cagtaacgtg actttcgtcc
     2161 accaccatca ggtaatcatc cgggaaacaa tcgagcaggc agaaaggcct tcccccgggt
     2221 ttacgaccat cgaaatagcg ggaataattc tctatgcccg aacaatagcc gagctcacgc
     2281 atcatctcga gatcgtacga cacgcggtcc tcaatgcgct tggcttccat catctttccc
     2341 atatctttca gatacgcaat atgggagatc aggtcgtgat gaatatctgt tattgccttt
     2401 ttcatcttat cctgagaaga aataaacagg ttagccgggt aaatggaaat cgcgtcaagt
     2461 ttttcgataa aacgcccgtt tacgggttca aacgtttcaa tagattcaat ttcatcgcca
     2521 aaaaagcata cgcgtatgca aaagtcggca taagcgggcg aaacatcaat agtatcgccg
     2581 cgaaccctga aattgccccg tccaaaagtt acatcattgc gcgaatataa aatattgacc
     2641 aaggcctgta aaaacttctt taccggaacg gtatcaccta ttttcatcct gatgatgctg
     2701 tttgcaaaat cttcgggatt gccaatacca taaatgcacg acaccgacga aactacaata
     2761 acgtctcgcc ttcccgataa taaggccgag gtggcgctca acctgagttt ttcaatttca
     2821 tcgttgatcg aaaggtcttt ttcgatatag gtattggtca caggcaggta tgcttcgggc
     2881 tgataataat cataatagga aataaagtac tccacggcat ttttgggaaa aaactgccgg
     2941 aactcactgt atagttgagc tgcaagggtt ttgttatggc tgagtaccag cgttggccgc
     3001 tgcacctcct gaataacatt ggctatggta aacgtttttc cggagccggt aacaccgagg
     3061 agcacctgcg cctcatcgcc gcgttgcaga ccttcggcga gttgcctgat tgcctcgggc
     3121 tgatcgccgg taggcacata atccgatgtc agttcaaatt tcaacgactt actgtttttt
     3181 cttttccgcc aggcacatta ccgggtagtg atcggaatta tccgttttta catttttaaa
     3241 attactgact ttaaaatggt tagacagaaa aatgtaatca atacggaatg atggatatat
     3301 tttataatac gtggtaccca acccgaaacc ggcctcttta aaagcatcat tgagcaggga
     3361 ccccaactga tgataggcgt atgaaaacgg cgtatcattg aggtcggtgc ataaaattac
     3421 cggataagga cattttttga tgtgcccggc cagcagttcg gcctgcaccg aacgcttgac
     3481 aaatcctctt ttgaggtacc gcaatatgcg taacgattta tttttcaaat ccttctcatc
     3541 ggtattggtg ttgcccgagg caatattatc ggcaaattca atgtcgctgt tgcccagatg
     3601 cagtgattgt aaatgggcat tgtaaatacg cacggtatca ttgctgataa ggacatccgt
     3661 ataaatacaa aagttatttc ttgaattggg aaattttatc agacccttcc ccacgatggg
     3721 aaacgtggta aaagtagcca ggccaaattc gtaaatatgc cttgaaacca cagaatattc
     3781 ggcatggaca ttggtggctt taagaaaagt gaccagggta tccttagtct taaattcgcc
     3841 gttacagtta tttacaaact cctgaaaaca aatgatatcg caatcctggt ttttcagaaa
     3901 agaaaaaatt ttattacgtt tttcataatt gatctgccag tttttcttgt agttatacaa
     3961 gtcgaaatta tgcacgttat acgacatgac tttgaaggcc aaaccgttac cattggctgg
     4021 cttttcggaa ttacactgca aaaaagaact gaaataactg aaacccagca agacaggcag
     4081 tagcgaaatc agggaatact taagccggat aaacagccac atcacaataa aagcgatgtt
     4141 tacaatcagc acataaggat aaatgattcc ggcaaaggct ggaagccaga acaccctcgg
     4201 attgatgaaa gaagagctga aagataccaa taacaacagc gccgcaagga tattggcaac
     4261 aagcaacagt ttccaaaaga aatgcagttt tttcgttgat ttctttgcca tggattactt
     4321 gtttttactt tctttaaaaa ggtattcctt atcctccttg ctcaggcttt cgtagcctga
     4381 tttttttatt ttatcaagta tattatcaat acgttgctgg tgggctttac gctcataacg
     4441 gtactcatca tcagttttgg gctttcgcgt atatgtgttt ttaacttttg gtttgcgaac
     4501 aaaccatttc ttgaatggat tggtaagttg cttgacaacc agccctttct tgagagctaa
     4561 aatatagaaa aaaccaaaaa aagcgccccc caggtgtgca atgtgtccac cgggattatt
     4621 attgaacgac tgtccgggta ttagcgtcca taggtcatag ataacaaaag ccagcgctac
     4681 ccatttgaat ttgacggcac caagaaaaaa cagcctgata ggataatcgg gaacataggc
     4741 cgcaatagcg ataatgatag ccataacaga ggcggaagcg cccaaagccg gaatgttgag
     4801 gtcctgggca aaagcaggaa aaacattata actaataata aagaacaaag ctcctgttat
     4861 accaccgaga atataaacca atagcaatcg cttgcttgac aggtattctg taaaaattac
     4921 cccgcccaca tacagcatca gcatattgaa aaataaatgc cagatacttt catgtgtaaa
     4981 catataagta ataatggtcc agggttttaa aatcagcaat tccaggtcgg ccggcacggc
     5041 cagccaatag cccggcacgg tgatacttcc ttcgtgaaat tgataaggac tgaccagata
     5101 caacttaaaa atcagtcgcg acaggttaat gataagaaag acagcaatat tgatgatgat
     5161 cagtatggac agggtggatt ttccggtaaa aaaacgtttc atgccgtcga gcattgaagg
     5221 acggcgttgt ccgtatggct ggccggaaaa attccggtta aagttgtaat aattattgcc
     5281 tgtcatgaaa ttgccttttt ttgtattgcc agcgccagaa caatatcatc gccaggccaa
     5341 acagcattcc ccccagatgg gcaaaatgag ccacattgtc gccctgtaca ttggaaatgc
     5401 ccgaaaccaa ttcgagggcg ccatagatta acacaaacca ttttgccttg atgggaatgg
     5461 cgaaatatac ataaatacgg gtattaggga acatcatgcc aaaagcaagc aatataccga
     5521 agacagcccc cgaagccccc accaccaccg gagcattcag taaatccatt ttgtattgca
     5581 ttacaaattc agcggaatag gtgattgctt tcgacatatc attgtttatc aacaagttgt
     5641 attgtgtgat aaaatttttt aagggtgggt aaccgccatc cacgtagcct gaattaagca
     5701 gttggctaag ctgggcatgg ccgggatcgg tgagataaac atccagtgcg tggatgacag
     5761 ggcgcaactc aaaaaacacg atggcataat gggtaacggc ggcaccgagg ccacagacaa
     5821 ggtaaaaaat cagaaaacgt ttcggccccc agaaattttc aagtacatag ccaaacatcc
     5881 acagcgcaaa catgttgaag aagagatgca taaaattgcc atgcatgaac atgtaagtaa
     5941 taaactggta aggcgaaaat ttttcggagg ccacatagtg caaacccagt ttgtcataca
     6001 gatcaatgcc gaacgcctga ccaagccctg tggtagccag aaaaaatata ccgttgataa
     6061 tcagaaggtt tttgactacc gtaggcagca tacgaaatcc ggtcgggcta tattgctcca
     6121 tccccatcga aacagaattt agttaaataa atgaattgca aagataggtg tttttctgca
     6181 tgtaaaaaca tggaaaacgg cagattatta ttagggagaa aaaattttcc ggccaatgcc
     6241 agaaaatgga tcagcagttc aaaactcctt taaaaacgag acttcctaat cagaaaccat
     6301 ataaattttc atcaataagt tttgccgcga cacgccggcg tgcatccttg atgttcagtg
     6361 gcttgactgc cgttaaggtt tccagggcgt ttttaagttt acaaaactcg tcgcccacgg
     6421 caaaggcaca gatggcatcg gtggctgatt ttctgatacg ggcggctgct tcatagaaaa
     6481 aggcatccat aatatcttta taaatcttta cctgttcttc gccttttatc tgctccagtt
     6541 ttttgacacg cagcatcagc gattcagcag caaaggtttg tatcagcata tcggaaatgt
     6601 tcatctgcac ttcctgttcc gaagaatatt gcttcccgag ggtttcgtaa gcccttccca
     6661 tgataagcag gatggttttc ttaaaattct caattacctt gcctgaataa tcaaaatatg
     6721 aagtttccgg agcctcatgt tgccaagctt tccggagggt ttccatgcct tcatataaag
     6781 ctgacgcctc ggcgaaaatc cgagaatcgg cttttttggc ttgcttcagc atcatatcag
     6841 cacataccat tcggttgatt tcgttggttc cttcgaaaat gcggttgatg cgcgagtcgc
     6901 ggtaacacct gtccacgggc atctcggccg aaaagcccat cccgccatga atctgaacag
     6961 cttcgtccac aatgtaatcc tgcgattcgg aagcaaaaac ctttagtatg gcggcttcca
     7021 tagcatattc gccaatggct tcgaggtagg cctgtgcttt agatttgcct tcggccacat
     7081 gaatgctgat ggtgtcatca acatatttgc tggccctata cactgccgat tcgaccgcga
     7141 acagcctgat gacctgttcg gccagtttgt attgtatggc accaaaggag gcaattttgg
     7201 aaccaaactg tttgcgttcg ttggcatagt tcaccgactt gttgatggta taacggcagg
     7261 ccccaagcac attagcgcca agtttgatac ggccaatatg caggatgttc agtgcgatac
     7321 ggaacccttc gcccctcttg ccgaggagat tgccgacggg cacttcgcaa tcgttgaaga
     7381 atatctgtac tgtggaagat cctttgatac ccattttatt ttcttcggca ccgaaagaaa
     7441 taccggggaa agtacgttca acaataaagg cgctgaggat gcgatcgtcg tcgattttgg
     7501 caaaaacggt catcacatcg gcaaaaccgc cattggtaat ccacattttt tggccgttaa
     7561 gtttataatg cgtgccatca gcagaaagca cagcccttgt ttttcctgaa ttagcatccg
     7621 aaccggcatt gggctcggta aggcaatacg ctccggcaaa ttcgcccgaa gccagtttgg
     7681 gcagatattt ttgtttttgt tcttcgtttc cgtaatacaa aatgggtaaa gagccaatcc
     7741 ctgtatgcgc cgaataagcg acagcgaaag aaaatcccgc tcccagccga tcgctggtca
     7801 gcatagaagt aataaaagac tgcccgaaac ccatatattc ttcgggtatg gaaatgccca
     7861 gcagccccag ctctccggct ttgctgagca agctgcgcat caaaccttcc tttttagtgt
     7921 caatttcgtc aagtatggga ataacttctt tttcgagaaa atcatcgcag gtatctgcaa
     7981 tcattttttg ttcttcgtta aattcttcgg gtataaaaat ttcggcggcc gctttggtaa
     8041 taaactctcc acctttaagg taattctgat cgttcataat attggatttg taagttaatt
     8101 aaaaaagagt ttacaaaatt agcatttttt ggcctgtaaa tctgtttaaa atttgtttaa
     8161 caactattaa catttttaat cgtcactatg aaaatttacg tatttttgaa atcatttttg
     8221 atcaaattat aattatgaga agattattta ctatttcttt tttgctttta tttacggttt
     8281 ttgctggtat atcacaaaac acacccaaaa cggatagcgt aaccaatcat tggggcataa
     8341 gtctatacgg ttctgccgat tacaatatgt acatggtaca aggaaccacc cctgcagggc
     8401 aggatgcagg attcatgaaa aacatcgacc tgcctcgcat tggatatacg ataggcgcct
     8461 cagtgaaccg tcctgtcggg aaaagggtat ccatggaatt tggtgtccag ttgcaaagcc
     8521 agggctaccg aagcaaaaac cttatcgata ccctctggaa ctaccgcgat tcagccattg
     8581 gctttagcga ctatgaaaaa gcatacaggt atttttccgt tgctattccg gtgatggtaa
     8641 aaatcaagct tttcgatatg ggcaaagctc atttggattt gggactgggt gtggcaccgg
     8701 tactctctgc cggaaaacaa caggccctgt ttttcgacga ccacactgaa atactcaacg
     8761 aaaaatcggg gacggccatt aatgttcaag ctttagggtg tctgagcttt tacataccca
     8821 tgggcaacaa aatgtcgctg acactcgaac ctacgttccg ctacaatatt cttccataca
     8881 aggacagcta ctacattggt gtgaaacgca acctgttctc agccggttta ggcatatata
     8941 tcacccgcaa agtaaccgat gatgagatgt acgattattt ttaccgccgc atttacaaag
     9001 taaaaaacct tccgccggca ttctgataac aaaaatttaa gccggttcca tgttaagtaa
     9061 agttcaaata aaaaaccgca gggcttcatt cgaatatttt attattgaag agctgacagc
     9121 cggtatagtg ctcacaggca cagaaataaa atcgctgcgc gatggcaagg cctccataaa
     9181 tgaggcatac tgcgttttta aggacaacga actttttgta atcaatatgc atattacaga
     9241 atactcctat ggtacctacc tgaaccattt gccgaaaaga gaacggaaac ttttgctcaa
     9301 caggcgtgaa ctgaaaaagt ggctgggcaa ggtcaaagaa aaaggattca ccatagtacc
     9361 caccatgctc catattaatg ataaaggcct tgcgaagctg aatattgcgc ttgcccgtgg
     9421 taagcattcc tacgacaaga gagaaacgct gaaagaaaaa gacatcaaac gggagatgga
     9481 caggaaagaa taagacatcc agccagtctc acaataatat ggaaagatgg ctttttttga
     9541 aaaaagcaca atctaccggt aacaataaag tactccctgc gacatttcat caatgtttga
     9601 tagaatgttc atcatccatc tgcaaaaaat catcctcctc atcttcctca tcatcctcat
     9661 cccagcgata cacatcacgt tgtattcctt ttttacgata atatacagca agcaccagac
     9721 ctgccagacc gcccatcagg tgcgactccc aggagatgtt gcgttttggg aaaaaatcgg
     9781 ggaacatccc ccagaaaaaa cctccgtaca aaaacacgat gagcagcgaa atagccatca
     9841 aacgggcatt acggcgaatg atcccgctaa aaagcaaaaa actcacatag ccgtaaacta
     9901 atccgctggc tccaatatgg tacacatcgc cccgcgcaaa agaccacacc cacacgcccg
     9961 taaacagcca aaccagcaaa aagacctggt aagccacctc attataaaaa taaaaaatta
    10021 agctcagcgt gataaaaagg gggatagtat tggcgatcag atgtccgaaa tcggcatgta
    10081 tgaatggtgc cgtaaccaca ccgatcaggc ctgcccctgt taacggcata ataccaaaat
    10141 cgctgaaatc tgatttggta taaaattcaa taattttgac aagccacatc acggtaacca
    10201 ctgcaaatgg gaacatcata ctggctaaca ttttcaggta ttctttgtgc atcggcagaa
    10261 aattaaggca cgcctaaaaa aatattctgt ttataaaaaa aaataaaagt acaaaaaact
    10321 caataatatt actttccatc cgttaaaaat aatagttctt tttatttaac tttgcatgcc
    10381 aacaattttt caattacaaa gcattactat gaaaaatcac tttaggacta tactattcag
    10441 acccctgttt ctgtttgtgc tctgctttac cttttatgcc aacaacggtt atgcccagca
    10501 acacgacccc aaaaagaccg aaaacaaatt tgctgcagcc ttgcagatta tcaggttgca
    10561 atatgttgat acaaccaacg aagaaaagct taccgaggtg gccatcagga gtatgctcaa
    10621 ggagcttgac ccgcattctg tttatatgtc gaagcaggaa attgacaagg ccaacgaacc
    10681 gctattaggc aattttgaag gtataggcgt tcagtttaat atcatcaaag acaccatcat
    10741 tgtgattact cccgttccca acggaccatc cgaaaaactt ggcatcatgt cgggagataa
    10801 aattgtgaaa attgatggcg aagatgccac cggcgaaaaa attaacagtg attttgtagt
    10861 aaaaagactt cgcggaccca aaggcagctc ggtgaaagtg agcatattcc gcaggggcaa
    10921 aaaagagttg ctcgattata ccattgtaag agacaagatt cccatcaaca gcctggatgc
    10981 cgcatatatg tccactgagg acatcggcta cataaaactc aataagtttg cccagaccac
    11041 catgtcggaa ttttcggaag ctctttcgaa actcaaaaaa caaggcatga aaaaccttat
    11101 cctggacctg cgcgacaata atggcgggta tctgaatacg gccatcgaac ttgccgatga
    11161 atttttgaaa aaaggtcagc tgattgttta taccgaagga gtccactcaa accgttcagt
    11221 taatcttgcc acagaaaaag gtaatttcga aaaagggaaa ctggtggtac tcatcaacga
    11281 aggttcggct tcggcaagcg agattgttac aggggccgtg caggactggg acagaggcct
    11341 tgtcatcggc agacgttcat tcggcaaagg gcttgtgcaa aggcctttta ccctccccga
    11401 cctgtcggta ataagactca ccaccgcaca ttattacact cctaccggtc gctgtgtgca
    11461 aaagccttac aacgacggta ttgaaaagta ctataaagac ctgaccaacc gttataaaca
    11521 cggtgaattg accaaccccg acagtataaa atttcctgat tccctgaaat attacacccc
    11581 gaataaccgc ctggtttatg gcggcggagg cattatgccc gacatattta tcccccttga
    11641 taccacaaaa gtttcggact tctatcaaga cctgctacgc aaaaatatca taaatctata
    11701 tgtactgaaa tatcttgata ttaaccgcga aacactaaaa aaacagtacc ctgacatgga
    11761 agtatttctg aaaggatata acctgaccaa tgactttatg caggattttc tgaagtattc
    11821 cgaagaagaa ggggttaaaa aggatgagga aggatataaa gtttcggaaa cctacatcaa
    11881 gaattttatc aaggcatgga ttgcccgcaa tttatgggat tacgacgcat actggcgcat
    11941 cagcaacgag caggacgatg tatatcttaa agctgttgag gcgatacaga gtaaagatat
    12001 gtttaagaac tacaagattt tttattagcc ggggacgttc ctgtgtgagc taaagtctgt
    12061 aagaaaaatc aatgtttatt ctctgaaatc aatcatcaca atcatcctga tagaggtcca
    12121 tatattcaaa aatgatgttg aaggcctcct gtttcatatc catctgcagt gatttatctt
    12181 tccgtaccct gtcggtgatt ttgaaaacca tgcaccaatc cccgtccata gccttctgcg
    12241 cttttatcaa cgcacaaata atgttataag tgaaactttt agggttcttg ctgacagcct
    12301 tttctgcata aacaatggcc tcgtccacct caaaataatt gtccattgct ttcaggtagg
    12361 ccatacataa caactcggac gcccctgccc ttttcaacaa atcgtctgtt cctttatagc
    12421 ctttgtgttc ctgcaaatag tcaaagaaaa cagaagcatt ttgttttcca tccaggctcc
    12481 atcccaaccg attgataaca gccattttca cagcaatagg ttttttcttt gccagcaaga
    12541 attttatcag ctgatttgtg agcaccccat cggttgccgc agccctgacg accatttttt
    12601 catgttgata agctttgtaa aagtctgttg atgttaaagg agaatcggca aataatgggt
    12661 tacagctgat ccacaaaaaa ctcaacaata acaggtactt tattttttcc ataaaaaatt
    12721 tttatcatta tactggtggc gccccaatga atcctgacaa aaaacgagaa attggtttag
    12781 aggaaaacca atagttgtga agttaaattt gaatatcagt aaactttgac tgtttttttt
    12841 ctttcgttga ccggtggagg agattccttt ttccagtttc ttttggcttt caggccaggg
    12901 tttacggaac agtttatttt cttggtattc tgccacctgc ccttttttac ggcatggttt
    12961 gaataagaaa gggcttttgt tttttttgcc cggtttaatt tggatttttg cttacggggt
    13021 ttccctgttt cgtatttgtg tactgcagta agctcatcac cggatgtgtt ttttgttaca
    13081 gttccgctgt ggtgagaaac tgccaaagaa tgccctgtac tccaaatgat aaaagccaag
    13141 gcaagaacaa ttgataaaac attatgcctg atcatattgt ctgtattaat tgcttttcaa
    13201 ttaataattt taatcaacat cattgattca gccaagaaaa agcacaaaat cctgcatgcc
    13261 aaatcagtgt aaaattaaac attttttttg aaatatccaa attttgatca aacatgaata
    13321 attcaaaggt aattttcacc cctggggctg gcatatgaaa atgggaaata atctacgaac
    13381 accatgatga atcagcgcga ttattagtga tagtacttag cttatcggcg ccaatatttt
    13441 gaatattttc aaggcggcca tttatcacag gccccctgat gccgttaaag aaaaaacaat
    13501 ttcctgaaaa caccctggct tcgtcccagc aaccctcatc aataaaagat tgcagggtaa
    13561 cacagccgcc ttcgactatc agtgactgga tgtttttttc atagagaacg gtcagcattt
    13621 cggggataat atttttgctg aaatccagtt ttttatattc aatatttccg cacactgctt
    13681 cttttacaga attaaaaact acggtttgtg ctgcattgtc cattattttg gtacttgtag
    13741 aaatggccag ttgccggtct attacgaggc gcaggggatt tttaccatac cattcgcgca
    13801 cagtgagttg cggatcatcc ttcagtacgg tatttttgcc agccagtata gcttgttctt
    13861 ccgagcgcca acgatgcacc agcaaacgga ttttctcg
//
LOCUS       DAOSFP010000006        13307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_3894_length_13307_cov_4.096212, whole genome shotgun sequence.
ACCESSION   DAOSFP010000006 DAOSFP010000000
VERSION     DAOSFP010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3894_length_13307_cov_4.096212"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1937)
                     /locus_tag="PLI16_00455"
     CDS             complement(132..1937)
                     /locus_tag="PLI16_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOH83058.1"
                     /translation="MVADVNNDDYPDLLSIWTGNIYGKDNMRVYINVQDTNSADPLDR
                     IFLDITPITGINANIIPDSAGHRILNAVFADVNNDGNIDMITGNDTYNLESYYDNGDR
                     GEVLLGDGTGAFLTVPDNGIHTAILNNPTGHSMLDYDRDGNLDVFMGTFFKNYNSNIW
                     NHGFLYKGNGDGTFTDVSSPAGLTVKNEPDYGCQVTDWNNDGWPDIITSPYCRNGGTL
                     WKNNGNGTFSDVSAITGYDTQFMQGDNGQNLCNWIAAPEDFDNDGDMDLMLALVHGGN
                     DANEGRSTIVINEGPTAGYHLHWDLNRVTWDAPKSTHHGDYDVSWFDFDNDGLEDMSL
                     QQGHYMPASDRLYMFYQNPDHTFSDINAELGFIGDTTFANTHASEPLDYDLDGDDDLI
                     FAKDGTVKTYLLRNEIGNQNNWVTVKLIAPAGCNYNSIGARIYVYADSLVKMRDVYAG
                     RGNKCGQQPFNLVFGLGAHNSIDSIVVRWPDSLFTVTTAVNPPINQIVVIDVNGYVAN
                     VPENHSDVAENIKLYPNPAQDHFVLQGINNYSLNGVAAIEIYDIGGRKISNFSYYNNN
                     STIAVVNVSKLDQGAYITRIISKEGGVITKQFIKM"
     gene            2353..2721
                     /locus_tag="PLI16_00460"
     CDS             2353..2721
                     /locus_tag="PLI16_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOH83059.1"
                     /translation="MDIKAPATVDEYIAQLTKEKSKLLKQIRVIVKEMAPDAMEVISF
                     RMPAYKQNGMLLFFAAHKNHIGFYPGISAVEAYKKKLEQYKTSKGTIQFPLDSPLPLE
                     LIKEIVVYRVNENLLKKKKK"
     gene            2718..3140
                     /locus_tag="PLI16_00465"
     CDS             2718..3140
                     /locus_tag="PLI16_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOH83060.1"
                     /translation="MNKPIYTCLWFDGQAHEAAEFYCSIFKNSSIISETPVVVTFQLN
                     GRRFLGVNGGPLYKFSEAVSLVVDCDTQEEIDYYWQRLTDGGEESMCGWLKDKYGVSW
                     QIIPEVLYQLMNDPVKAPLVFEKFSKIRKFIIADLLRL"
     gene            3343..3777
                     /gene="tnpA"
                     /locus_tag="PLI16_00470"
     CDS             3343..3777
                     /gene="tnpA"
                     /locus_tag="PLI16_00470"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOH83061.1"
                     /translation="MPHSYNKIWIHAIWSTKYRDQLIMPKIEFQVYQYMREQFIESGC
                     PVRIISGMPDHVHCLFLLNPQKSIAEIIKQIKGSTSHFINEQNLIEGKFSWQTGYAAY
                     SVSESAVEKVFEYIKNQKSHHAKMTFLQEYNDFLKLYGFENG"
     gene            complement(3949..5259)
                     /gene="miaB"
                     /locus_tag="PLI16_00475"
     CDS             complement(3949..5259)
                     /gene="miaB"
                     /locus_tag="PLI16_00475"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOH83062.1"
                     /translation="MKKTFYIETYGCQMNFSDSEIVASILTKENFSEAATPEDAGLIL
                     LNTCSIRENAEQRIFNRLRHLKSLKKKNKGLMIGILGCMAERLKEQLFEDGIVDIVAG
                     PDSYRELPQLIFQACEHGQAFNILLSAEETYADINPVRIDSNGVSAFISIMRGCQNFC
                     SYCVVPYTRGKERSRSSETIISEATGLFNDGFREITLLGQNVNSYNYNDINFPKLLSE
                     VALINPALRIRFATSHPKDLSDELIDVIASHDNICKSIHLPLQSGSNRILKLMKRNYT
                     RESYMDIIEKIYARIPGCSLSTDIISGFCSETEQDHEDTLDIMRRAAYDSAFMFKYSE
                     RQGTFAAARYKDDVPEDVKTRRLNEIIALQNKLSLAKNKKDVGKTFEVLIEGRSKKSE
                     SQLSGRNSQNKVVVFTDDKHKAGDYVMVKITGCTSATLKGEILP"
     gene            5377..7827
                     /gene="topA"
                     /locus_tag="PLI16_00480"
     CDS             5377..7827
                     /gene="topA"
                     /locus_tag="PLI16_00480"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOH83063.1"
                     /translation="MIKNLVIVESPAKAKTIEKFLGEDFTVRSCFGHIRDLSKKDLGV
                     DIQNGFIPQYEIPDDKAKIVGELKKMSKDAQTVWLASDEDREGEAISWHLSEALQIDK
                     KKIKRIAFHEITKTAIQEAIAQPRQINIDLVDAQQARRVLDRLVGYELSPLLWKKIRP
                     SLSAGRVQSVAVRLIVERENEINNFKSVSFYKGSAIFSFVKDNKTYRIKAELSRKFTA
                     PGNAMDFLKKCIGAQFHVKDIETKPAKKSPAPPFTTSTLQQEASRKLGFSVSKTMLIA
                     QQLYESGKITYMRTDSVNLSKMALAMAKNEIESFYGPGYVKIRNYITKSKGAQEAHEA
                     IRPTYINNHVIEGNSDQKKLYDLIWKRTIASQMSDAELEKTNVYIESPSISDYFLATG
                     EIVKFDGFLKVYLESTDDEEEEQKGILPPIAQGDKMHPDEINVVERFTQQPPRYTEAS
                     LVKKLEELGIGRPSTYAPIISTIQKREYVTFGNKEGAERKYLFLSLINDKIQETQKTE
                     NFGYEKAKLFPTDVGTLVNNFLIQNFENILDYNFTASVEKEFDEIAEGKKAWNEMIRE
                     FYFPFHNQISETEKTSKKVSGVRLLGFHPESGKNIYAKLGKFGGMLQLGENTDEEKPR
                     FVGLLKGQSIETISLQEALSLLSFPKLIGTYQDTEIVAGLGRFGPYIRHDGKFYSLKK
                     TDNPITLTLSRAIEIINEKISKIENSTIKEFAENPGVRVLKGRFGPYISMNKNNYKIP
                     KEYNPADLTIEDCMRIAEEASGKEGAKKRPAKKTGIKKTVAKKTAAKKTITKKTAAKK
                     TTVKKAVKKSVAKKTAKK"
     gene            7883..9055
                     /locus_tag="PLI16_00485"
     CDS             7883..9055
                     /locus_tag="PLI16_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOH83064.1"
                     /translation="MDISLYFEPVNINQVYDEEQQHPNHIKNALSIYTKENSFPSLDG
                     VDMAIMGVNESRAAIGNSGCKGAPGQVRKYFYPLFKHHNIKIADLGNVNPGKAINDTY
                     FAVSDIAAALLKKNIIPLIIGGSQDITYANYLAYESIGRIINLVCVDMAFDLGAIDSP
                     LTSKTFLSNIITHQPSFLFNYSNIGYQSYFVDKEAVALMDKLFFDVYRLGLVQTDIEE
                     VEPMVRNADILSVDIAAVRKSDAPGNGCATPNGFYGEELCRIMRYAGLSDKLTSVGLY
                     EINPEFDHYGQTASLAAQALWYFIDGFYSRKQDFPIKDKKFYIKYRVAIKELQNEIVF
                     YKSKKTNRWWMEVPCPTRLKSKYERHYLVPCSYKDYETAMKEDIPDRWWQAFQKIM"
     gene            9405..10409
                     /locus_tag="PLI16_00490"
     CDS             9405..10409
                     /locus_tag="PLI16_00490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOH83065.1"
                     /translation="MKKIYFFIVVSVFSLSVFGQQDAQFTQNMFTNMFVNPGYAGISK
                     QICLTTLYRQQWTGFTDSYLEGGETKTHKGAPQTILLTVDAPIRVLHGGIGLSVFSDK
                     LGFENNIGVKLAYSFHVNIAIGTLGLGLQAGFLNKSIDFSKFNPLDQNDPLLMSNSGK
                     KSTFMTDIAFGAFYNIPGKAYVGLSADQLIESKIKLAGESKSKLKRHYYLTGGYHWTL
                     PNNPDWELSPYVLIKTDFASAQYDITAMCKWRNMIWAGVSYRVQDAVAIIVGAYPLNQ
                     PSMSPALQNLRIGYSYDITTSALGRNKRSSGSHEVMLGYCFKIEIKKNITKYLNTRYL
                     "
     gene            10518..10994
                     /locus_tag="PLI16_00495"
     CDS             10518..10994
                     /locus_tag="PLI16_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH83066.1"
                     /translation="MKKFLIIIVIFASLAFTGCKNNGNGELTGVSGREGFYQPTPYGM
                     LFIPPGSYQMGPSDQDVPYSLTAQHKTVTVQAFYMDETEITNNEYRQFVYYVRDSLAR
                     ELLGSLAPEDWWIEEDEYGEPLEPHHLNWETRLEWDDLEQEEIEKLKAEGKFAGRS"
     gene            complement(11079..11687)
                     /locus_tag="PLI16_00500"
     CDS             complement(11079..11687)
                     /locus_tag="PLI16_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83067.1"
                     /translation="MDRIIIAPPMGAIYQRLGFRKKATQISDDARKETDRHIEEARSL
                     IALKGLYLRLAVSGNDGQTIILEGGLSFSSAKLATFLGTSREAILMGATAGNVIMEAI
                     REKTNRQEMAAAVVYDATASEMADAALDWITGFINRQIRREGKTMLERRFSAGYGDFA
                     LENQKLIYDALRMDQIGVAISERFLLIPEKSVTALSGIRPIL"
     gene            complement(11914..12315)
                     /locus_tag="PLI16_00505"
     CDS             complement(11914..12315)
                     /locus_tag="PLI16_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH83068.1"
                     /translation="MKGKILIVDDEASARELFSDVFGKEGYEVIAAEDGNRALDILKQ
                     DNIDVIFLDLKLFGMNGIDLCRQIRVTKPVSIIFAITGWAPLFEIEECREAGFDDYFE
                     KPLDMDFMLAVVEDAFKKLERWKRRYNNIQA"
     gene            complement(12345..>13307)
                     /gene="leuB"
                     /locus_tag="PLI16_00510"
     CDS             complement(12345..>13307)
                     /gene="leuB"
                     /locus_tag="PLI16_00510"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOH83069.1"
                     /translation="EQGLIGGSAYDAFGTPLPEESLKLALASDAVLLGAVGGLKWEGL
                     DYSVRPERALLGLREKLGLFANLRPVVVFNELLDASPLKKEVVQGVDIMIVRELTGDV
                     YFGRPRGVTRDDQNRRTGVNTMVYTEGEVHRIAVRAFELARARRKKLMSVDKANVLET
                     TELWRDVVTEAGRDYPDVELGHMYVDNCAMQLVRNPSQFDVIVTTNLFGDILSDEAAM
                     ITGSIGMLPSASLGRDRALYEPIHGSAPDIAGKGIANPLAMILSVAMMLRHSLGAPDA
                     ALAVERAVTGVLQEGLRTSDIQMAGGRTVGTKEMGEALIRKLLP"
BASE COUNT         3954 a         2999 c         2785 g         3569 t
ORIGIN      
        1 gatacagcgc acgatgccac ccactgcgca atagttcact acgttcctat tgctcaatct
       61 gggattattc tgaagataca gtgcttgccc gcaaattcag caacctaaat ttattcaggc
      121 caccggcatg attacatctt tatgaattgt ttcgtaataa ctccgccttc tttcgatatg
      181 atacgggtta tataagcgcc ctgatccagt tttgagacgt tcactacagc gatcgtgcta
      241 ttattgttgt aatagctgaa attactgatt tttctgcccc caatatcgta tatctcaata
      301 gcggcaacac cattcaaaga ataattattg ataccttgca gcacaaaatg atcctgtgcc
      361 ggattgggat acagttttat gttctccgcc acatcactat ggttttcagg aacattagca
      421 acatatccat ttacatcaat aacaactatc tgattaatgg gcggatttac cgctgtggta
      481 accgtaaaca atgaatcagg ccagcgaacc actatagagt caatagaatt atgagccccc
      541 aacccgaaaa ccagattaaa aggttgctgc ccgcatttat ttccccttcc ggcatacaca
      601 tctctcattt ttaccagaga atctgcataa acatagatcc ttgcaccgat agaattatag
      661 ttacatccgg cgggagcaat tagtttaacg gtaacccagt tgttttggtt gcctatttca
      721 tttcgtaata aatatgtttt tacagtgcca tctttagcga atataagatc atcgtcaccg
      781 tcaagatcgt aatccagggg ttccgatgca tgtgtatttg caaacgtggt atcgccaata
      841 aatcccagtt cggcattaat atccgaaaaa gtgtgatcgg gattctggta gaacatgtac
      901 aacctgtcgg aggcaggcat ataatgtccc tgctgaagcg acatatcttc aagcccgtcg
      961 ttgtcgaagt cgaaccacga cacatcataa tcaccgtgat gcgtcgattt cggagcatcc
     1021 caggtaaccc tgttcagatc ccaatgcaaa tgatagcctg cggttgggcc ttcattgatc
     1081 acgattgtgc tgcggccttc gttggcatca tttcctccat ggacgagtgc aagcatcagg
     1141 tccatatccc cgtcgttatc aaaatcttca ggagcagcta tccagttgca gagattctgt
     1201 ccgttatcgc cttgcataaa ttgtgtatcg tagccggtta tggccgacac gtcggaaaaa
     1261 gttccgttac cgttattttt ccataatgtt ccgccatttc tgcaataggg cgaggtaata
     1321 atatccggcc agccatcgtt gttccagtcg gttacctgac agccgtaatc aggttcattt
     1381 ttaacagtaa gtccagccgg agaggaaacg tccgtaaagg tcccgtcacc gttgccttta
     1441 tagagaaacc catgattcca gatgtttgaa ttataattct taaaaaaggt acccataaaa
     1501 acatccagat tgccatcacg gtcataatcc agcatcgaat gtcctgtagg attatttaaa
     1561 atggccgtgt gaatcccgtt atcaggtact gtcaggaatg ctcctgtgcc atcgcccagc
     1621 aatacttcgc cccggtcacc attatcatag taggactcca gattgtacgt atcgttccct
     1681 gtaatcatgt caatattccc gtcattattc acatcggcaa atactgcatt caaaatccgg
     1741 tgtcctgccg aatcgggaat gatattagca ttgatcccgg taatcggtgt tatatcaaga
     1801 aatattctgt caagcggatc tgctgaattg gtatcctgca cattgatata aactctcatg
     1861 ttgtctttgc cataaatatt tccggtccag atgcttagca ggtcgggata atcatcgttg
     1921 ttgacatctg caaccattta ataaaaagat tttacaccac taaggcccac ttgatttgta
     1981 acatttttga accatgtagt gtcttgtgcg tagttttgat cgttacaaag aaacagtcct
     2041 aggattgtga ttaataatag gtaaattttt ttcataaaaa ttaataatta aaagtgtttc
     2101 taataaaaaa ccagggcgcg gttaataata aaccaaaggt atttaatggt tttctaatta
     2161 acaaataatt tttcaacatt aatattttta ttttatattt gatgaataat aatagccaac
     2221 ggattgaccc gttggctgtg gaaaaaaact catatttatc aaaatcattt caatggttta
     2281 taaattttat tcgaataaaa attcaaacaa ataaaaaata attgaaacag tataatataa
     2341 aatccgcata aaatggacat aaaagcccct gctactgttg atgagtatat tgctcaactt
     2401 acaaaagaaa aaagtaaact gctgaaacaa ataagagtta ttgttaaaga aatggctcct
     2461 gacgctatgg aagttattag tttcaggatg ccggcatata aacaaaacgg gatgttactc
     2521 ttttttgcag cgcataaaaa tcacattgga ttttatcccg gtatttcagc tgttgaggcc
     2581 tataaaaaga aactggagca gtataaaact tctaaaggga ctattcaatt tcctctggat
     2641 tcgccactgc ctcttgagct tataaaagaa atcgtggtat atcgtgttaa tgaaaatttg
     2701 cttaaaaaaa agaaaaaatg aacaaaccga tctatacatg tttgtggttt gacggacaag
     2761 cccacgaagc agcggaattt tactgctcca tttttaaaaa ttcatcaata atttctgaaa
     2821 cacctgtggt tgtcaccttt caattgaatg gtcgccggtt tttaggagtg aacggtggcc
     2881 ctttatataa gttttctgaa gctgtttctc ttgttgttga ttgtgataca caggaggaaa
     2941 ttgattacta ttggcagcgc cttactgatg gtggcgaaga gagcatgtgt ggctggctca
     3001 aagataaata tggagtttca tggcaaatta ttcccgaggt gctttaccag ctaatgaatg
     3061 atccggttaa agcaccgctc gtttttgaaa agttttcaaa aatcaggaag ttcatcatcg
     3121 ctgatttatt gcgattatga tttaaaagtc agcagttgaa tttaattggc tatggaaaaa
     3181 attcatattc atcaaaaccg tttcaacggt ttattaaatt tattcaaaaa aaaatggtta
     3241 aaaccattac aaaaaaatca tagccaacgg tttaaaccgt tggctattgg aaaaaaacat
     3301 attcatcaaa accgtttcaa cggtttataa aaaaatcata ccatgccaca ttcctataat
     3361 aaaatttgga tacacgccat ctggtcaacc aaataccggg atcaattaat tatgccaaaa
     3421 attgaattcc aggtttatca atacatgcgg gaacaattta ttgaatcagg ctgtcccgta
     3481 aggattatta gcggaatgcc cgatcatgtg cattgtttgt ttttgttaaa ccctcaaaaa
     3541 tcaattgctg aaataattaa gcaaataaag gggagcacat ctcatttcat aaatgaacaa
     3601 aatcttattg aaggtaaatt ctcatggcaa acgggttatg ccgcttattc ggtaagtgag
     3661 tctgcagttg aaaaggtttt tgaatatatt aaaaatcaaa aatcacatca tgcaaaaatg
     3721 acctttctcc aggagtataa tgatttttta aaattatatg gatttgaaaa tggttaaaac
     3781 cattacaaaa cacaacaatt ctaccatgag gctgtctcaa aactaccttt gctgaaaatc
     3841 atcattaccc catccctgaa gggagtgctg attttcagcg acttccaccc tttagggagg
     3901 ggaaaaagaa ggcggtgaaa atcataaaat caaccttttg agacagcctc atggtagaat
     3961 ttctcccttc agcgttgccg aagtacaccc tgtaatcttc accattacgt aatctcccgc
     4021 tttatgcttg tcgtcagtaa ataccacaac tttattctgc gagttccttc ccgaaagttg
     4081 tgactccgat tttttcgaac gcccttcaat taatacttca aaggttttac ccacatcctt
     4141 cttgtttttc gccagcgaaa gtttgttctg caacgcgatg atttcattca accgcctggt
     4201 ttttacatct tcgggcacat cgtccttgta tcgggcagca gcaaaagttc cctgacgttc
     4261 cgaatacttg aacataaagg cgctgtcgta agcagcacgc cgcatgatgt cgagggtgtc
     4321 ttcgtggtcc tgttccgttt cgctgcaaaa accgctgatg atatcagtcg agaggctgca
     4381 tccggggatt cgggcatata ttttttcgat aatgtccatg taagattctc gtgtgtaatt
     4441 tcgtttcatc aatttcaaaa tcctgttact gcccgattgc agtggcaggt gaattgactt
     4501 gcaaatatta tcatgcgagg caataacatc aataagttca tcggataaat ctttcggatg
     4561 cgacgttgca aatcgtatcc tcagcgcggg gtttatcaag gcaacctcgc ttaataattt
     4621 aggaaaattg atgtcattat aattgtacga gtttacattt tggccgagta acgttatttc
     4681 gcgaaacccg tcattaaata atccggtagc ctcgctgatg atggtttccg aagaacggct
     4741 gcgctctttg ccccgggtgt aaggcaccac gcaataagaa caaaaattct ggcaaccgcg
     4801 catgatcgag ataaatgccg aaacgccatt actgtcaatc ctcaccgggt tgatgtcagc
     4861 ataggtttct tcggccgaaa gcaatatatt gaatgcctgg ccatgttcgc aggcctggaa
     4921 aatcaattgt ggaagctcgc ggtatgaatc cggcccggca acgatatcta caatgccatc
     4981 ttcgaatagc tgttctttaa gcctttcggc catacacccc aaaatgccta tcatcaaacc
     5041 cttgtttttt ttctttaggc tcttcaggtg acgaaggcgg ttaaagattc gctgttccgc
     5101 attttctctg atagaacagg tgttgagcaa aatcaacccg gcatcttccg gggtggctgc
     5161 ctccgaaaaa ttttcttttg ttaaaatgga agccactatt tcgctgtccg aaaaattcat
     5221 ctgacagcca taggtctcaa tataaaacgt ttttttcata cccgcaaatc ggaggcaaaa
     5281 ttagcgaaac aactcattga aaaaaaataa attgtcgctg aaaaattttt tttgttttat
     5341 tttgcacaac ttttttactt acccaaaaat acattcatga taaaaaatct tgtaattgtt
     5401 gagtcaccgg caaaagccaa aaccatagaa aaatttcttg gtgaagactt cacggtaaga
     5461 tcatgtttcg ggcatatccg cgacctgtcc aaaaaagatc tgggtgttga tattcaaaat
     5521 gggttcatcc ctcaatatga aattccggat gataaggcca aaatcgtcgg cgagttgaaa
     5581 aaaatgtcca aagatgccca gacggtgtgg ctcgcctccg atgaagaccg cgaaggagaa
     5641 gccatctcgt ggcaccttag cgaagccctc caaattgata agaaaaagat aaaaagaatt
     5701 gcatttcacg aaatcaccaa aactgccatc caggaagcca ttgcccaacc ccggcagata
     5761 aacatcgacc tggtggatgc acagcaggca cgccgggtgc tcgacaggct ggtggggtac
     5821 gagctgtctc cgttgttatg gaaaaaaatt agaccatcat tgtcggcagg ccgtgttcag
     5881 tctgttgccg tgaggcttat cgtagaacgc gaaaatgaaa taaacaactt taaatcagta
     5941 tcgttttata aaggatccgc catattttcc tttgtgaaag ataataaaac ctaccggata
     6001 aaagccgaac tatccagaaa attcactgct cctggcaatg ccatggattt tttgaaaaaa
     6061 tgtatagggg cacaatttca tgtaaaagac attgaaacca aacccgccaa aaagtcgccc
     6121 gcaccgccgt tcaccacttc gaccttacaa caggaagcca gccgtaaact ggggttttcg
     6181 gtgtcgaaaa ccatgctcat cgcccagcaa ctttacgagt cgggaaaaat aacctatatg
     6241 cgtaccgatt cggtgaacct gtcgaaaatg gcgcttgcca tggcgaaaaa tgaaatcgaa
     6301 tccttttatg gccccggcta tgtaaaaatc cgcaattata tcaccaaaag caaaggggca
     6361 caggaagccc acgaagctat caggcctact tatatcaata atcatgttat tgaaggcaac
     6421 tccgatcaga agaaacttta tgacctgata tggaagagaa ctattgcctc gcagatgagt
     6481 gatgctgaac ttgaaaaaac aaatgtatat atcgaaagcc cgtctatcag cgattatttt
     6541 cttgctaccg gtgaaattgt aaagttcgac ggatttctga aagtctatct cgaatccacc
     6601 gatgatgagg aagaagaaca aaaaggtatt ttgccgccta ttgcgcaagg cgataaaatg
     6661 caccccgatg aaatcaatgt ggtggaacga tttacacagc agcctccacg atataccgaa
     6721 gccagcctgg tgaaaaagct tgaagagctt ggcatcggca gaccgtcaac ttacgcacct
     6781 atcatttcca ccattcaaaa aagagaatac gtcacctttg gtaataaaga aggcgctgaa
     6841 cgtaagtacc tcttcctctc acttatcaat gataaaatcc aggaaacgca aaaaactgaa
     6901 aattttggtt acgaaaaagc caaacttttc cctactgatg taggtacgct ggtaaataat
     6961 ttcctgatac agaattttga aaatatcctg gactataatt ttacagccag cgtggaaaaa
     7021 gagtttgatg aaattgccga agggaaaaaa gcctggaacg aaatgatcag ggagttttat
     7081 tttcctttcc acaatcagat cagtgagacg gaaaaaactt ctaaaaaggt tagtggcgta
     7141 aggctcctgg gtttccatcc tgaaagcggc aaaaacatct atgccaaact gggcaaattt
     7201 ggaggtatgt tgcaactggg cgaaaatacc gatgaggaaa aaccgcgttt tgttggcttg
     7261 ctgaaaggtc aaagcatcga aaccatcagc ctgcaggaag ccctgtcttt gttgagtttt
     7321 cccaaactca tcggcaccta ccaggataca gaaattgtag ccggattagg tcgttttggc
     7381 ccctatatcc gccacgatgg taagttttat tccttgaaaa aaactgataa tccgatcacc
     7441 ttaaccctca gccgggccat cgaaatcata aatgaaaaaa tttcgaaaat agaaaacagc
     7501 accataaagg aatttgcgga aaaccccgga gtacgcgtat taaaagggcg tttcggaccg
     7561 tacatctcga tgaataaaaa caactacaaa ataccaaaag aatataatcc tgctgactta
     7621 acaatcgaag attgcatgcg gatagccgaa gaggcttccg gaaaagaagg cgcaaaaaaa
     7681 cgaccggcaa aaaaaacagg cataaaaaaa acggttgcca aaaaaaccgc agccaaaaaa
     7741 accataacca aaaaaaccgc agcaaaaaaa acaaccgtta aaaaagcggt gaaaaaaagt
     7801 gttgccaaaa aaacggcaaa aaaataatgc ccgtttttaa gtgaatggta cgataataca
     7861 aaaggaaaaa tacctgacaa aaatggatat ttctttatac ttcgaaccgg tcaacatcaa
     7921 tcaagtatat gatgaagaac aacagcatcc caatcatata aaaaatgcgt tgtccatata
     7981 taccaaggaa aattcatttc catcgctcga cggtgttgat atggctatta tgggcgtaaa
     8041 cgaaagccgc gcagccattg gcaatagcgg ttgtaaaggt gctcccggac aggttaggaa
     8101 atatttttat cccttgttta agcaccataa cataaaaatt gccgatctgg gcaatgtaaa
     8161 tcccggtaaa gccattaacg acacctattt tgctgtgagc gacattgcag ccgccctgct
     8221 gaagaaaaac atcattcccc ttattatcgg tggcagccag gatataacct atgccaacta
     8281 tcttgcctat gaatccattg gccggattat caaccttgtt tgtgttgata tggccttcga
     8341 tttaggggcc attgacagcc cactgacatc aaaaactttt ttaagcaata tcattaccca
     8401 ccagcccagc ttccttttca attattccaa cataggttat caatcttatt ttgtcgataa
     8461 agaagccgtg gctcttatgg ataagttgtt ttttgatgtg taccgtctgg gcctggtcca
     8521 aaccgatatc gaagaagtgg aacccatggt gcgtaatgcg gatattcttt cggtggatat
     8581 tgctgccgtg cgtaaatccg atgccccggg caacggctgt gccacaccca acggatttta
     8641 cggcgaagaa ctgtgtcgca tcatgcgata cgccggatta agcgacaaac ttacctccgt
     8701 gggattatac gaaataaatc cggaatttga ccattatggc cagacggcaa gccttgcggc
     8761 acaagcctta tggtatttta tcgacggatt ttatagccgg aaacaggatt ttccgattaa
     8821 agataaaaag ttctatatca aataccgggt agctatcaaa gaacttcaaa acgagatagt
     8881 tttttataaa agtaaaaaaa caaaccgctg gtggatggaa gtgccatgcc ccacgcggct
     8941 gaaatccaaa tatgaaaggc attacctcgt tccctgttca tacaaagatt atgaaacagc
     9001 catgaaagag gatatcccag accgctggtg gcaggcgttt cagaaaatta tgtaagccgt
     9061 tttttctgcc tttcatctac ttctgttatc tttagtttta ttttctctta gcaaacgctg
     9121 ttaattattt gttgaaaaaa aatgtttttt catgggcatt tttttatcaa tcagttgatg
     9181 aattcctgaa aaaaattatc tttgcatttg aaactttttt gtgcagcttt ccgtaaaaaa
     9241 acaatgtgaa aaatttttct gacaagaaat tttaactatt tgtttatgag ggttttacaa
     9301 aaaagcgaat cttgtataat taaaaattgt taacaattgc ttgttaatat taaaaaatat
     9361 gcgttacttt atccccattt ttgaatttta tcaataaaaa ccatatgaaa aaaatctact
     9421 tttttattgt tgtttccgtg ttttcattgt ctgtttttgg ccagcaggac gcacagttca
     9481 cgcaaaacat gtttacaaat atgttcgtga atccgggtta tgcaggcatc agcaaacaga
     9541 tatgcctcac aacactgtac cgtcagcaat ggacaggttt tacggattcc tatctcgaag
     9601 gcggtgaaac aaaaacacac aaaggcgctc cccagaccat tctgcttacc gtggatgctc
     9661 caatacgtgt tctgcatggt ggtatcggcc tgtcggtttt cagtgataaa ctcggatttg
     9721 aaaacaacat aggtgttaaa ctggcctatt cttttcatgt caatattgcc ataggaacgc
     9781 tgggtttggg attgcaggcc ggattcctga ataaatcaat agatttttcg aaattcaatc
     9841 cgctggatca gaatgatcct ttgctcatga gcaatagcgg caaaaaaagc acatttatga
     9901 ccgatatcgc tttcggtgca ttttataata ttcccgggaa agcctatgtg ggcttatcgg
     9961 ccgatcagct tatcgaaagt aaaatcaagt tagccggaga atccaagtcg aaactaaaaa
    10021 gacattacta cctcaccggc ggttaccact ggacactgcc caacaaccct gactgggaac
    10081 tcagtcctta cgtattgata aaaacagatt ttgcctctgc acagtatgat attaccgcca
    10141 tgtgcaaatg gagaaacatg atctgggccg gcgtttctta ccgcgtgcag gatgccgtag
    10201 ccattatcgt gggcgcctac ccgctaaatc agccttccat gagcccggca ctgcaaaatc
    10261 tccgtatcgg atattcttat gatatcacaa cctcggcgtt aggaagaaat aaaagaagta
    10321 gcggatcaca cgaagtaatg ctcggttatt gctttaaaat tgagataaag aagaatatta
    10381 cgaaatattt gaatacaaga tatctgtaaa aaaaattacg ttgattgtaa caaaaagatg
    10441 tacttttacg ttttaatgtt accaacgggc aaaaaaggaa cataaaaagc gaaaaaatag
    10501 taagtaaaaa aaagaatatg aaaaaattcc tgataatcat agtaattttt gcaagcctcg
    10561 ctttcacagg atgcaagaat aatggtaacg gagaactcac gggagtttcc ggaagggaag
    10621 gattttacca gccaacacct tacggcatgt tgtttatccc cccgggaagt tatcagatgg
    10681 gccccagcga tcaggatgtg ccttattcgc tgacagcaca gcataaaacc gtgaccgttc
    10741 aggcttttta tatggatgaa actgagatta ccaataatga gtatcgccag tttgtatatt
    10801 atgtcaggga ttctttagcc agagaactgc tgggaagcct tgcccccgaa gattggtgga
    10861 ttgaagaaga tgaatacggt gaacccctcg aaccgcacca cctgaattgg gaaacccgcc
    10921 tggaatggga tgatctggaa caggaagaaa tagaaaaatt gaaagcagaa ggtaaattcg
    10981 cgggcaggag ctgaagccgc ctggagtgca atcataccgg tatcaggtcg tgcagggccg
    11041 gacggcgcgg ctccacccgg aacgccgcag tcggtaaatc acagtatcgg ccggatgccg
    11101 ctcaaggcgg tgaccgattt ttccggtatc agaagaaagc gttcgctgat ggcgacaccg
    11161 atttggtcca tcctgagcgc atcatagatg agtttttgat tttccagcgc aaagtcgccg
    11221 tagccggcgg aaaagcgtcg ttcaagcatt gtcttcccct cacggcgaat ctgtcggttg
    11281 ataaagcctg tgatccagtc gagcgccgca tccgccattt cgctggcggt cgcatcatag
    11341 acgacagccg cggccatctc ctggcgattg gttttctcac ggatcgcctc catgatgaca
    11401 tttcctgcgg tggcccccat gagtatcgcc tctcggcttg tacccaggaa ggtggcaagt
    11461 tttgccgagc tgaaagacag gcctccctcc aggatgatgg tttgtccgtc gttacccgaa
    11521 acggcaaggc gaagataaag ccctttaagt gcaatcagtg accgagcctc ttcaatatgg
    11581 cggtccgttt cctttcgtgc gtcatcgctt atctgtgtgg ctttttttct gaaacccagg
    11641 cgctggtaga tggcgcccat cggcggggcg atgatgatcc tatccaaaat ttttactgat
    11701 tcgtgcatga cggctccgcg aaaaagccct gatgagtatc gacggctgca tcatctattc
    11761 aatgcgcctc tggcggcgcc tcgccgctta ttcgcccgaa actgacaaat gaccgtccgg
    11821 ctgttgcctc ccttcggcaa ccgccccggc catgagcgcg gggattacag acagaagagg
    11881 cccggacaaa accgctcctc atcgcctttg atattatgcc tgaatgttat tgtagcgcct
    11941 cttccagcgt tccagctttt tgaaggcatc ttctacaacg gcaagcatga agtccatatc
    12001 gagaggtttt tcgaagtagt cgtcaaaacc cgcctcacga cactcctcaa tttcaaaaag
    12061 aggggcccag ccggtgattg cgaaaatgat cgacactggc tttgttacgc gaatctgacg
    12121 acaaagatca atgccgttca tgccgaagag cttcaaatcc agaaagatga catcaatatt
    12181 gtcttgttta agaatatcca gcgcccggtt gccgtcctcg gcggcaatga cttcataacc
    12241 ctctttgccg aagacatcgg agaatagttc ccgggccgag gcctcgtcgt caacaatcag
    12301 tattttccct ttcaaagccg caaactcctg ttcatgttaa cggttcaggg cagaagcttc
    12361 cggatgagcg cctcccccat ttcttttgtg cctacagtcc gccctcccgc catttggatg
    12421 tcggaagtcc tcaacccctc ctgcaatact cctgtcaccg ctctttccac ggcaagcgcc
    12481 gcatccggtg ctcccaggga gtggcgcagc atcatggcca cagacaaaat catggccagc
    12541 gggttggcaa tacctttccc ggcaatgtcc ggggcggatc cgtgaatcgg ctcgtacagg
    12601 gcacggtccc ggcccaggct ggccgacggg agcatgccga tggaaccggt gatcatggcc
    12661 gcctcgtcgc ttaatatgtc gccgaagaga ttcgtagtca cgatcacgtc aaattgcgac
    12721 gggttgcgga caagctgcat cgcgcagttg tccacataca tgtgaccgag ctccacatcg
    12781 ggataatccc tgccagcctc ggtgaccacg tcgcgccaca gctctgtcgt ctccagcaca
    12841 ttggccttgt ccaccgacat gagctttttt ctccgcgcac gcgccagctc gaatgccctc
    12901 acggctatcc ggtgaacttc cccctcggtg taaaccatcg tgttgacacc ggtgcggcgg
    12961 ttttgatcat cccgcgtcac accgcgggga cggccgaaat atacatcgcc ggtcagctcc
    13021 cgcacgatca tgatgtcaac gccttgaacc acttcctttt tcagcggtga tgcatcaagc
    13081 aactcgttaa agaccaccac cggtcgtaaa ttagcgaaaa gccccagttt ttccctgagc
    13141 cccaaaaggg cgcgctccgg tcggacactg tagtccagcc cttcccattt caggccgccg
    13201 accgccccca gaagcaccgc gtcactggcc agtgccagct tgaggctttc ctccggcagg
    13261 ggggtgccga aggcatcgta ggcgctgccg ccgatgagcc cctgctc
//
LOCUS       DAOSFP010000007        13245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_3920_length_13245_cov_4.347157, whole genome shotgun sequence.
ACCESSION   DAOSFP010000007 DAOSFP010000000
VERSION     DAOSFP010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3920_length_13245_cov_4.347157"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PLI16_00515"
     CDS             complement(<1..1364)
                     /locus_tag="PLI16_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83070.1"
                     /translation="MMIHGRKPFRRRYIAMPIQLIRNSVLLVLILLAFTTRAQYYDLG
                     QDPASVRWKVIKTEHFKIIFPAGIEDQAQHIANGFEKVYAPLSADMKIAAVKVPVVLH
                     NRAILSNAEVPWAPKRIEFFMCPGQDDYAQPWADQLMLHEFRHVVQYSKINQGLTRGL
                     SYIFGQQITAGVIGTFVPLWFVEGDAVYTETVFSNSGRGRLPSFYMKMRAQVLEKGLY
                     NYNKACFGSYRTFTPNRYEFGYQLVATARRHFTGDVWEKAMDKAGKLPLMVTPFNYGL
                     KKVSGMGKVKLYKYCFYELDSLWHHQRIQTNVTVAEKIPVAKSKIYTNYNFPALAGEK
                     SYIMVRSGMNDITRFVRIDSAGREHKLFTLGQYSFASFSVHGTKMVWAETANDPRWEN
                     QTYSVIKIYDMQKRRARQLTRRSRYFSPAIDRQGKKIVCAEQGITGQSALVVLDAATG
                     KVLSR"
     gene            complement(1376..2473)
                     /locus_tag="PLI16_00520"
     CDS             complement(1376..2473)
                     /locus_tag="PLI16_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83071.1"
                     /translation="MKKLFTVLFFVIFCLNANAQTLTSVADGNWNNPLTWGGNLPIPG
                     STIIINHHIVLNTDYGFTSGSITINSGGSLTGDSGLRGLSMNYPSGSGFLTINGAMDV
                     ARVSFFDDTVTVEGSFDADSLYNKAVIDVGLSGTINASQFFNGFTGNINNSGSIHAIN
                     LLNLGSIVNAGVINAADFDNCKSLTNNAGAMIALSHNFLNADSISGPAIFTNNGRQEV
                     MNDWQNKDTVYGAGKFCIVNNTSNSGLMSGTFDFCDMSGGDVDFNTGDIDTTITYCAF
                     PCTPGTAEFAGDYLIEIFPNPAGEIFYVSSEKEISSIEVFNLLGETVYSATVNARKFK
                     VCMPLISNGIYLYRLSDKDNSTKTGKIIIEK"
     gene            2604..2963
                     /locus_tag="PLI16_00525"
     CDS             2604..2963
                     /locus_tag="PLI16_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOH83072.1"
                     /translation="MKFIIKVLVTALAVMAAAYILEPHVRVESFWISIIVALVLALLN
                     AFVRPILLLISIPVTILTLGLFIFVINVLIIYIAAYFIDGFEVDGFLWTLLFSIIISF
                     FSSILNSILKTDSPQKN"
     gene            2985..4142
                     /locus_tag="PLI16_00530"
     CDS             2985..4142
                     /locus_tag="PLI16_00530"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH83073.1"
                     /translation="MDISDIINHLGEDRRDYYGAVAPPVMRTSNFCFASVEDMRSNIN
                     REFEVPFYSRGNNPTVSILRKKMAALEKTEDALITASGCAAISTAVFGNLAAGDHAIC
                     VTNCYSWTYTLFHDILKKFGVNVSFIDGTRIEHFKNAITPKTRLIMLESPTSFMMELQ
                     DLKAVAALAKQHEITTIIDNSYATPLYQNPAELGIDIVVHSASKYLGGHSDLIAGVIC
                     GTEKMIRKLFSQEYLAFGGIITPDNAWLILRGLRTLPVRMKQISETTQKIVCFLEDHP
                     KIDKVLWPFAKSYPKYELARQQMKGAGGLFSFTLKTKEMEKVDMFCNKLRRFLLACSW
                     GGYESLVFPGSVLYSSMNNNKAIPFNLIRMYAGLEEPAVLIQDIEQALEYV"
     gene            4154..4462
                     /locus_tag="PLI16_00535"
     CDS             4154..4462
                     /locus_tag="PLI16_00535"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOH83074.1"
                     /translation="MQLNIIHWLEHHTTPCLFKSIFGLDCPGCGTQRAIIELLKGNFT
                     ESLKAWPALLPVTLMLCYLALFLVFRFKNGHKILIVFFIVNAIIIAGNYIFKFAIHAL
                     "
     gene            4545..4916
                     /locus_tag="PLI16_00540"
     CDS             4545..4916
                     /locus_tag="PLI16_00540"
                     /inference="COORDINATES: protein motif:HMM:NF040945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCC motif membrane protein"
                     /protein_id="HOH83075.1"
                     /translation="MEEQQTDFSLPPQKEYKRHVLRPKMIPYSVTAMVFGIVSIVVLC
                     YFGWVAAIVALVLYNKGMKIYNENPPDYKPGSLGMMRAAKITGIIGLILSIVVTLLYI
                     LYVVFIIGLVNYSSDSFLLNP"
     gene            4913..6421
                     /locus_tag="PLI16_00545"
     CDS             4913..6421
                     /locus_tag="PLI16_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOH83076.1"
                     /translation="MKILTAVQVREADAFTIANEPISSVDLMERAAGKCFNWLVQHYG
                     KNRNYKIFCGTGNNGGDGLVIARLLAEKNIPAETFIVRFSDKSSEDFITNHQRLLKIK
                     KIKITDIATTADIPVIAGKDIVVDAILGSGLSKPLTGGIAADVVEAINKSTAEIVAVD
                     IPSGLFGEDNSDNNGKIIQARQTLTFQFPKLSFLFAGNEKYAGNWHVLPIGLHPDFIE
                     KTTVNNYFVEEADCRAIIKPRSRFSHKGMFGHALLVCGSYGKMGAAVLAAHACIKSGA
                     GLVTSHIPKAGYKIMQCSLPEIMCSIDEDKHVISGNIDLDTYNAVGVGPGIGTDDKTQ
                     KAMKLLIQNCISPLVFDADAINILAENKTWLSFVPKKSIFTPHPKEFERLAGKSNNEY
                     QRQALQTAFSKKYGVYVALKGAYSCISTPAGDCYFNSTGNPGMATAGSGDVLTGIITG
                     LLAQKYTPFDACILGVYVHGLAGDLSAKRNGYEATTAGEIIKMLGKAFKKLY"
     gene            complement(6413..6958)
                     /locus_tag="PLI16_00550"
     CDS             complement(6413..6958)
                     /locus_tag="PLI16_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH83077.1"
                     /translation="MKVTDAIMQRFSPLAFTDEIPGEEILMQLFESARWAPSAFNEQP
                     WRFVCTSKKEPEKYSKLFDCLVDANKEWAKTAPVLILVMAKKNFSYNSKPNYFHMYDT
                     GMAVSNLIISAMEKGIYVHQMGGFSAEKARENFNIPDEYAIISVAALGYQGDIAVLPA
                     ALQEKVKKARTRKPLGELFLV"
     gene            7475..7978
                     /locus_tag="PLI16_00555"
     CDS             7475..7978
                     /locus_tag="PLI16_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOH83078.1"
                     /translation="MRKILGFLIAALIVTSCGNSTGETEKKDDAAADTEKKAEQVVSK
                     PTTIDEATFRAKVFDYTSEKKFKYLGEKPCIIDFYADWCAPCRKLAPILEAIAAEYGE
                     KLIVYKINTDHNQQVSQYLNISSIPAVLLCPMNGDPQMMVGLYPKQEYINAIEQVLFP
                     VAPTTAK"
     gene            complement(8006..8869)
                     /locus_tag="PLI16_00560"
     CDS             complement(8006..8869)
                     /locus_tag="PLI16_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH83079.1"
                     /translation="MAKANQVLMEIYFITAEHVKFDGGACFGVVPKSLWGKIYQPDEH
                     NMVDVALRNLLVVENGRKILFDTGVGNKQDEKFRSYFVLRGEDNLVNALEAHGFSTDD
                     ITDVVHTHLHFDHCGGSFRYGDSNQPYPVFKNATLWCSQRQWNWANNPNPRERASYLK
                     ENLLPLQQSGKLNFVADETLFTEKIFLKMVNGHTDGQLIPVIDYHGRKLVFMADFIPT
                     TAHISLPYIAGYDTRPLLSMEEKEGFLAEAVENHYLLVFQHDALNECCTVQQTEKGVK
                     VKEKGRLSDFL"
     gene            complement(8983..11028)
                     /locus_tag="PLI16_00565"
     CDS             complement(8983..11028)
                     /locus_tag="PLI16_00565"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83080.1"
                     /translation="MKKIIPLIFTLCLSLPVFSQNHAPTAVNDYVVTKRGHVIVDILA
                     NDFDVDGDSINILFILPPNHGRIIRLSDREIEYTAFPSYRGGLDSIRYKLMDNGSPVL
                     SSYGYVIFDVDNPLSFDSVAVNNISACVNADGFLFAEYVRISSTSTNIYPWFSVPKGK
                     KTKTIYSGNVWIGGLDDQDSLHVAAQRYYTYGCDFWAGPVSSAYDSLYDDKYKRLWKI
                     NRSDIDYHLAHWQQPGYTPSQILLDWPGNGDVLKGESPVLAPFYDNNNDGIYDPYTGD
                     YPLIKGDETVLFIFNDDREQHTETGGKKLGIEGVGMLYGFDCPSDSALWNTVFLNVKI
                     NNRSVNTYHNSYIATYTDFSLGDPTDDYIACDVQRGSFYCYNGDNIDGYGRPQDYGAH
                     PPAQAITFLGGPYLDSDGLDNPAGNCDESVNGLNFGNSVIDDERHGMSSSGPHWCFSC
                     TLEFCPSTDVDYYNYMRGFWGGGAQMHYWGNGHPDQSATGPACYFMFPGDTDPCNWGT
                     RGIDPGGTEPWTEEQAGNLPYDRRSAGSSGPFTFFPGAIQELDLAYVFARNFADTNAK
                     AAITVMNQRIDSVRSYFIKDATPCGEKFSMYKPEVTNSDFLYLYPNPASEFVELEYFY
                     NSYSRFEIYDALGHLVKSGDLTETRKQLLDISSLRKGLYFIRITGGIDSVVKKFVKY"
     gene            complement(11369..>13245)
                     /locus_tag="PLI16_00570"
     CDS             complement(11369..>13245)
                     /locus_tag="PLI16_00570"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOH83081.1"
                     /translation="CGFDAIQNQSSVEKDYLTNGFWAFNMKEELPCTGVTASFLVRME
                     DVTSTVLAAVNDTMSESARSSAIKKVSDKLEKDASEKGKYFVDVKSFFDNNEYYMFVY
                     IIYKDVRLVGAPPSAIGKFGGDTDNWMWPRHTGDFSMLRIYTAPDGSPAEYSDKNIPL
                     KPKHHLPVSLKGIKKGDFAMIWGYPGTTERYMTSYGIQTTLEQTNPAIINAGNIMLPI
                     MKKDMEADPAVRIKYSASYASLANVWKNKIGESRGLKRLKVYDKKKALENELQKWIDA
                     DESRKAKYGNVIKDIEEGNKQLLANKSEANFWYFQMILMSSKTLLAPLYTMGMSSLVE
                     NKNTKPEMFNDYKETIKDLYKDANTSTEKKLFAAGLEILRKEMRADIFDVIDKNFKGD
                     IQKFTDEAFKTSIYASEEAFNAFVSKPNAKKFSNDLVRKTTMSILNYYRNSAGEQKAI
                     ESKLNKATRLFSEALRKMQPDKKFYPDANSTLRMTYGKVLDYEAADAVHYDYFTTLDG
                     VMEKEDASNSEFVVPEKLKQLYKNKDYGQYGNNGVMPVCFISNLDITGGNSGSPVING
                     DGQLIGIAFDGNWEAMSGDIAFEPEVQRTISVDIRYVLFVIDKYAGAKNLIDEMTIVK
                     "
BASE COUNT         3845 a         2940 c         2725 g         3735 t
ORIGIN      
        1 cggctcagca ccttgcccgt agccgcatcc agcaccacca aagcagactg gccggtgatg
       61 ccctgttcgg cacaaacaat ctttttgcct tgcctgtcta tagccgggga gaaatagcgc
      121 gaacggcggg taagctgcct ggcccgtctt ttttgcatat catagatctt gatcaccgag
      181 taagtttggt tttcccatcg gggatcattg gcggtttcgg cccacaccat cttcgtgcca
      241 tgcacgctga aagaggcaaa ggaatactgg cccagggtaa atagcttgtg ctcccgtccg
      301 gcactgtcaa tacgtacaaa acgtgtgatg tcgttcatgc cggaacgaac catgatatag
      361 gatttttcgc ctgctaaggc aggaaaatta tagttggtat aaatcttgct tttcgctaca
      421 ggaatctttt ctgcaacggt aacattcgtc tgtattcgct gatgatgcca aaggctatcc
      481 agttcgtaaa aacagtattt gtacagcttt acttttccca tgccggacac ttttttcagc
      541 ccgtaattga aaggggttac cattagtggc agtttgccgg ccttatccat agccttttcc
      601 cagacatcgc cggtaaagtg tctgcgtgcc gtagccacca gttgataacc aaattcatac
      661 ctgttaggag taaatgttct gtaagagccg aaacaggctt tgttgtaatt atataatccc
      721 ttttccagca cctgtgcgcg catcttcatg tagaaagaag gtaagcggcc gcgcccgctg
      781 ttgctgaaaa cggtttcggt atataccgca tcgccttcca caaaccacag tggcacaaag
      841 gtgccaatca cgccggctgt gatctgttgt ccaaagatat aggacaaccc acgggtaagc
      901 ccctggttga ttttgctgta ctgcaccaca tgcctgaatt cgtgcagcat aagctggtcg
      961 gcccacggct gggcatagtc atcctgtccg ggacacataa aaaattcgat gcgcttgggc
     1021 gcccaaggca cttccgcatt ggataaaatg gcgcggttat gcaacaccac tggcactttc
     1081 accgctgcga ttttcatatc ggcgcttaat ggtgcataga ctttctcgaa accattggcg
     1141 atatgttgtg cctggtcttc aatgccggca ggaaaaatta ttttgaaatg ttccgtttta
     1201 ataaccttcc accgcacaga ggcaggatcc tggccaagat cgtaatactg tgccctggtt
     1261 gtaaaggcaa gcaaaatcaa cactaacaag acactatttc gtatgagctg tattggcatg
     1321 gctatatacc gcctgcgaaa tggtttccgg ccatgtatca tcattatcgg gaggcttatt
     1381 tttcaataat tattttccct gttttcgtcg aattatcctt gtctgacagc cggtaaaggt
     1441 aaatcccatt tgaaatcagc ggcatacaaa ccttgaattt acgggcattc acagtggcgc
     1501 tgtacactgt ttctcccagc agattgaaaa cttcgattga cgaaatttct ttttcagaag
     1561 aaacataaaa aatctctcca gcagggttgg gaaaaatttc aatcagataa tcccctgcaa
     1621 attcggccgt gcccggggta cacggaaagg cgcaatacgt aatggtcgta tcgatgtccc
     1681 ctgtattgaa atcaacgtct cctcccgaca tatcgcaaaa atcaaaagtg cccgacatta
     1741 atccggagtt gctggtattg ttcacgatac aaaattttcc ggcaccatac actgtatctt
     1801 tgttttgcca gtcattcatc acctcctgcc ttccattatt ggtgaagata gccgggccgg
     1861 agattgaatc ggcattaaga aaattatggc tcagggctat catggcgccg gcattattgg
     1921 taaggctctt acaattatca aaatctgctg cattgatcac accggcattt actatggatc
     1981 caagattcag aaggttgatt gcatgtatgc ttccggaatt attaatgttt cctgtaaagc
     2041 cattaaaaaa ctgcgaagca ttaatcgtac cgctaaggcc cacgtctatt acggccttat
     2101 tgtataaact gtcggcatca aaactgcctt ccacagtaac cgtatcgtca aaaaatgaca
     2161 ctcttgccac atccatggct ccgttgatag tgagaaagcc gctgcccgaa ggataattca
     2221 tggacagtcc gcggaggccg gaatcacccg tcagggagcc gcccgaatta atggtaatgg
     2281 acccggaggt gaacccatag tcggtattga gcacaatgtg gtgattgata ataatagtgc
     2341 tgcccggtat aggcaggttg ccgccccagg tcaatgggtt gttccagtta ccatctgcaa
     2401 cagaagtaag cgtttgcgca tttgcattta aacaaaaaat gacaaaaaag agaaccgtaa
     2461 atagtttttt cattacatgt gtttttgatg atgactacgt tggcagaaca aatttaaaaa
     2521 aagttcatgt aatacaggca ataaatttcc gtaaaacttc ttattactat gtttttaatg
     2581 tatttttgta aaaaaacaag cttatgaaat tcatcataaa agtactggtt accgcgcttg
     2641 ctgtgatggc tgcagcatac attctggaac cgcacgtccg ggttgaaagt ttctggataa
     2701 gcattatcgt agctcttgta ttggcactgt taaatgcctt tgtaaggcct atactgcttt
     2761 taatcagtat ccccgtaacc atattgactc tgggtttatt tatttttgta atcaatgtgc
     2821 taataattta tatcgccgca tattttattg acggatttga ggtggatgga ttcttgtgga
     2881 ccttgttgtt cagcattata atttcattct tcagcagtat tttgaacagc atattgaaaa
     2941 cagacagtcc acagaaaaat taatgcaggc caccacctca caacatggat atttctgaca
     3001 ttataaatca tcttggcgaa gaccgcaggg attattacgg tgctgtggcg ccgcccgtga
     3061 tgcgtaccag caacttctgt tttgccagtg ttgaagatat gaggagcaat atcaacaggg
     3121 aatttgaagt acctttctat tcaagaggaa acaacccgac tgtcagcata ctccggaaaa
     3181 aaatggctgc gctggaaaaa acagaagatg cgctgatcac cgccagcggc tgtgccgcca
     3241 tcagcaccgc agtgttcggc aaccttgcgg ctggcgacca tgcgatttgt gtaacaaatt
     3301 gttacagctg gacatacaca ctttttcatg acatactgaa aaaattcggt gtaaacgtca
     3361 gttttattga cggcaccaga atagaacact ttaaaaatgc catcacgccg aaaacacggc
     3421 tcatcatgct cgaaagcccc acctctttta tgatggaact ccaggacctg aaagccgtgg
     3481 cagctttggc aaaacagcat gaaataacca ccataatcga caacagctat gccacaccgc
     3541 tgtaccagaa tcctgccgaa ttaggaatcg acatcgtggt gcattctgcg tccaaatact
     3601 taggcggcca cagcgacctc attgcaggtg taatctgtgg taccgaaaaa atgattcgca
     3661 aattattttc acaggaatac cttgcttttg gcggcattat aacaccggat aacgcatggc
     3721 tgatactgcg cggattaaga acattgcctg tgcggatgaa acagatcagc gaaacaacgc
     3781 agaaaatagt ctgttttctt gaagatcatc caaagattga taaggtatta tggccatttg
     3841 caaaaagcta tcctaagtac gaactggcca gacaacagat gaaaggagcc ggaggactgt
     3901 tttcatttac tttaaagaca aaagagatgg aaaaagtgga tatgttctgt aacaaactga
     3961 gacgattcct gctggcgtgt tcctggggag gttacgaatc ccttgttttt ccgggctcgg
     4021 tgctttacag ctccatgaac aacaacaaag ctattccgtt taatctgatc cggatgtatg
     4081 caggactgga agaaccagca gttctcatac aggatattga acaggctttg gaatatgtat
     4141 aacttaatga gccatgcaac taaacatcat ccactggctc gaacatcaca caacaccctg
     4201 cctttttaag agtatttttg gccttgattg tccgggctgc ggcacacaaa gagccatcat
     4261 tgaactgctg aaaggaaact ttactgaaag cctgaaagcc tggcctgcct tgctgccggt
     4321 gacacttatg ttgtgttacc tggccttgtt tctggttttc cgctttaaaa acgggcacaa
     4381 aatcctgatt gtatttttta ttgtgaacgc aataattatt gccgggaatt acattttcaa
     4441 atttgccata catgcactat aatcgataaa aggcatgtag catttcgtgc taaaaacttt
     4501 ttcacaatga ataaaaacca ttaactttgt tataaaaaaa aaccatggaa gaacaacaaa
     4561 cagatttttc attgccaccg caaaaggaat ataaaagaca tgtgctcaga ccaaaaatga
     4621 ttccctattc cgttaccgcc atggtgttcg gaatcgtttc catcgtggtg ttgtgctatt
     4681 tcggctgggt ggccgccata gtagccttgg tactttacaa taaaggcatg aaaatataca
     4741 atgaaaatcc gcccgactat aagccgggat cgctgggaat gatgcgcgca gcaaagataa
     4801 caggcattat cggattgata ctttccatcg tcgttaccct gttatatatt ctttacgtag
     4861 tgtttatcat tggccttgtc aactattcct ccgacagttt tcttttaaat ccatgaaaat
     4921 tctaacggca gtacaggtac gtgaagcaga tgccttcacg atagccaatg aacctatctc
     4981 atcggtggat cttatggaac gtgccgcagg caaatgtttc aactggcttg tacagcacta
     5041 cggaaaaaac agaaattaca aaatattttg tggtacaggc aacaatggcg gcgacggcct
     5101 ggtgattgcc cgcctgctgg ctgaaaagaa tattccggca gaaactttta tcgtccgttt
     5161 cagcgataaa agctccgaag actttattac caatcaccag cgtttattga aaataaaaaa
     5221 aataaaaatc accgacatcg ccactacagc agatattccg gtcatagcag gaaaagatat
     5281 cgtggtggat gccatactgg gttcgggact ctcgaagccg cttacaggag gtattgccgc
     5341 cgatgtggtg gaagctatca acaaaagcac cgctgaaatt gttgcggtgg atatcccctc
     5401 cggcctgttc ggtgaagaca atagcgacaa caacgggaaa attatacaag ccagacaaac
     5461 cctaacgttt caattcccca aactgtcgtt tctttttgca ggcaatgaaa aatatgccgg
     5521 caactggcat gtacttccca tcgggcttca tcctgatttt atagaaaaga ctacagtaaa
     5581 caactatttt gtggaagaag ccgactgcag ggcgatcatc aaaccaagga gccggttttc
     5641 gcacaaggga atgttcggcc atgccctgct ggtatgcgga tcctatggca aaatgggtgc
     5701 tgcggtgctg gctgcccacg cctgcataaa atcaggagca ggcctggtaa cgtcgcacat
     5761 tcccaaagcc ggttataaaa tcatgcagtg cagcctgccc gaaatcatgt gcagcataga
     5821 tgaagacaag cacgtcatct caggcaatat cgacctcgat acttataatg ccgtgggagt
     5881 tggccccgga atcggcacgg atgataaaac tcagaaagcc atgaaactgc tgatacaaaa
     5941 ctgcatttct ccgcttgttt ttgatgccga cgccattaac atcctggccg aaaacaaaac
     6001 ctggctgtct tttgtaccga aaaaatccat ttttacacca cacccgaaag aatttgaacg
     6061 cctggcgggc aaaagcaaca atgaatatca gcgtcaagct ttacaaactg ctttttcaaa
     6121 aaaatacgga gtgtatgtgg cactaaaagg agcctattcc tgtatcagca ctcccgcggg
     6181 tgactgctat tttaattcca ccggaaatcc gggtatggca acagccggca gcggcgatgt
     6241 gcttaccggt atcatcaccg gcttgctggc acagaagtac acaccttttg atgcctgtat
     6301 tcttggtgta tatgttcacg gcctggcagg cgacctgtcg gcaaagcgca atggttatga
     6361 agccaccacg gcaggagaaa tcataaagat gctgggaaaa gccttcaaaa aactatacta
     6421 aaaacaattc gcccaggggc tttctggtcc tggctttctt aaccttttcc tgcaacgcgg
     6481 caggcaacac ggcaatatcg ccctggtacc ctaaggcagc caccgaaatt atggcatatt
     6541 catcagggat attaaaattt tcgcgggcct tttctgcact gaaaccaccc atctgatgca
     6601 cataaatgcc tttttccata gcactgatga taagatttga aacggccatg ccggtgtcgt
     6661 acatgtgaaa ataattaggc ttgctattgt aggaaaaatt cttttttgcc atcaccagga
     6721 tcaataccgg cgctgttttc gcccattcct tattggcgtc caccaggcaa tcaaagagtt
     6781 tgctatattt ttcaggttct tttttgctgg tgcagacaaa cctccacggt tgctcattaa
     6841 aggccgaagg cgcccagcgg gcagattcaa acaattgcat aaggatttct tcgcccggga
     6901 tttcatcggt aaatgccagt gggctgaatc tttgcataat ggcgtcggtt actttcataa
     6961 gtgttttgtt ttaggttgca aaattactca ttgttccctg ataatgtaag ctaaatttga
     7021 taatcactgt tgtccggtaa aatctgaaaa attgatgaaa agccatattg gaatgaggtt
     7081 ttgcgtagat gtttattcaa taggggtttg tttttagcaa agggtgaatt gcttttcagc
     7141 aatttatttt acctcagtcc taaaggatgt aaattgctga aaatcaattc ttcctttagg
     7201 acagaggtaa ataacggatc tttttattta catcctttat tacaagttta atttttaccg
     7261 gacattaata tttgataata taatcacagg aaatccccct ctttaaagct attaaaaatg
     7321 atgttttgtt aaatttaatt tcagcgtccg gagttatgga ttcattacct gctgaaatcg
     7381 tgtaaaaagc tacggggtgc cttctgccaa cagattaatt ataacaaccc accaaaacgg
     7441 agaagatatt ttgtaacttt gcaaaaaaat ttttatgaga aaaatccttg gttttcttat
     7501 tgctgcgctg atagttacct catgcggaaa cagcaccggt gagacagaaa aaaaagatga
     7561 cgctgccgca gatacggaaa aaaaagccga acaggtagtt tctaagccga ctacgattga
     7621 tgaagcaaca tttcgcgcga aagtttttga ttataccagt gaaaaaaaat tcaaatatct
     7681 tggtgaaaag ccctgcatta ttgattttta tgctgattgg tgcgctccct gccgtaagct
     7741 cgcccctatc ctggaagcaa tagctgctga atacggtgaa aaactgatag tttacaaaat
     7801 caataccgac cacaaccagc aggtatccca atacctgaat atcagcagca ttcccgcagt
     7861 attgttgtgt ccgatgaacg gcgatccaca gatgatggta gggttgtatc ccaaacagga
     7921 gtatattaat gctattgaac aagtcttgtt tcctgtagcc ccaacaactg caaaataatt
     7981 attcgtaaat tcagtgaaaa gcggtttata aaaaatccga caaccttcct ttttctttca
     8041 ctttcacgcc tttttcggtt tgctgcaccg tacaacattc attcaaagcg tcgtgctgaa
     8101 acaccagcag gtaatgattt tcgacggctt cggccagaaa gccttctttt tcttccatgg
     8161 aaagcaacgg gcgggtgtcg tagcctgcaa tataaggcaa tgaaatatga gcagtcgtgg
     8221 gaataaaatc ggccatgaac accagcttac ggccatgata gtcgatcaca ggaatcaatt
     8281 gcccgtcggt atggccattt accattttta aaaaaatttt ttcggtaaac agggtttcat
     8341 cagctacaaa attcaacttg ccactttgct gcaacggcag aaggttttct ttaagatacg
     8401 aagctctttc acgggggttg ggattgttgg cccagttcca ctgcctttgc gaacaccata
     8461 gcgtggcatt tttgaatacc ggatatggct gattactatc gccgtacctg aaagagcctc
     8521 cgcaatggtc gaaatgcagg tgggtgtgca ccacatcagt gatatcatcg gttgaaaacc
     8581 catgagcttc gagtgcatta acgaggttat cttcaccacg cagaacaaag taacttctga
     8641 atttttcatc ctgcttatta ccgacaccgg tatcaaacaa aatttttctt ccgttttcaa
     8701 ccaccagcag gttgcgcaaa gcaacgtcca ccatattgtg ttcatcgggc tggtaaattt
     8761 tgccccacaa cgatttgggg acaaccccaa agcaggcgcc accgtcgaat ttcacatgtt
     8821 ccgcagtaat aaaataaatt tccattaaaa cctgattagc ttttgccaca aaggtaaaag
     8881 tttctgtttt attgaaatag aataaacatt atcgttttaa cttagctaaa attgggttaa
     8941 aaaaattcct catttgaaaa tccaattgct taaaatgaga aatcagtatt taacgaactt
     9001 tttgacgaca ctgtcaatgc caccggtaat tctgataaaa tacaatcctt ttcttagaga
     9061 ggaaatatcg agcagctgtt ttctggtttc cgtaagatca cctgatttca caagatgacc
     9121 aagtgcgtcg tagatttcaa agcgtgaata actgttatag aaatattcaa gttctacaaa
     9181 ctccgaagcc ggattgggat ataaataaag gaaatctgaa tttgtcacct cgggtttata
     9241 catggaaaac ttttctccgc atggagtggc gtcttttata aagtaactgc gaacgctgtc
     9301 aatacgttga ttcatgacag ttatggcggc ctttgcattt gtatctgcaa aatttctcgc
     9361 gaacacatag gcaaggtcaa gttcctgtat cgctccagga aaaaaagtaa atgggcccga
     9421 agaaccagcg cttctgcgat cataaggtaa attgcccgcc tgttcttcgg tccatggttc
     9481 ggttccaccg ggatcaatcc ctctcgtccc ccagttgcag ggatcggtat cgcctggaaa
     9541 catgaagtaa catgccgggc cagtagcact ttggtcggga tgaccattac cccagtaatg
     9601 catttgggca cctccgcccc aaaatccgcg catataatta taataatcaa catcggtaga
     9661 aggacaaaac tctaatgtac atgaaaaaca ccaatgagga ccggagctgc tcataccatg
     9721 acgttcgtca tcaataaccg aatttccgaa attcaagcca ttcacacttt cgtcacaatt
     9781 acctgctgga ttatccaatc catccgaatc caaataagga ccgcctaaaa atgtaatcgc
     9841 ctgtgcagga ggatgagccc cataatcctg cgggcgaccg taaccatcaa tattatcacc
     9901 gttataacaa taaaaacttc cccgctgaac atcgcaggca atataatcat cagtaggatc
     9961 acctaaactg aaatcagtat atgttgcaat atatgaatta tggtaggtgt ttacagaacg
    10021 gttgttaatt ttaacgttca aaaatactgt gttccacaaa gcagaatctg aaggacagtc
    10081 gaaaccatag agcatcccca ccccttcaat gccaagtttt ttcccgcctg tttccgtatg
    10141 ctgttcccga tcgtcattaa aaataaaaag gaccgtttca tcacctttta ttaatggata
    10201 atctcccgta taagggtcat aaattccgtc gttattgtta tcataaaatg gtgctaatac
    10261 aggagattct cctttcagca catcaccatt tccgggccag tcgagtagaa tttgtgaagg
    10321 agtgtaaccc ggttgctgcc agtgagcaag atggtagtca atatctgacc tgttaatttt
    10381 ccacaacctt ttgtatttat catcatataa agaatcataa gcagaagaca ccggccctgc
    10441 ccagaaatcg caaccataag tataatacct ttgtgctgca acatgcaggg aatcctgatc
    10501 atccaatcca ccaatccata catttcctga ataaatagtt ttggtctttt ttcctttcgg
    10561 gacgctaaac cacgggtata tattagtact tgtgctagaa atgcgtacgt attcggcaaa
    10621 taaaaaccca tcagcgttaa cacaggctga aatattgttt accgctactg aatcaaaact
    10681 taatggatta tcaacatcaa agataacata tccgtatgaa ctcagtacgg gactcccatt
    10741 gtccataagt ttataacgaa ttgaatccag gcctccgcga taacttggaa atgcagtata
    10801 ctcaatttcc cggtctgaca atcttattat acgtccatgg ttcggtggta aaatgaaaag
    10861 aatattaata ctatcgccat ccacatcaaa atcattagct aaaatatcta ctatcacatg
    10921 tccgcgtttt gtaaccacat aatcattcac ggctgtcggc gcgtggttct gtgaaaaaac
    10981 cggcagggaa agacaaagag taaatattaa tggaatgatt tttttcataa agaaagaata
    11041 tagtagcaaa tatatggaaa taaaataact tttataatcc cgaattacgc ataaaaggtt
    11101 ttctagcacg tcgcattaga acactttctt catagcatcc tgattattgg catattagct
    11161 aaacaatttt gtgtgtaaac gacaaagaaa atgtaatccc gacaaagaaa atttgtcgag
    11221 aaacattttc taagtcggga taatcccaac taaagcaata gattggttat catttgtaca
    11281 agttcgatca cccctattgc ttaaattggg ataataaaaa aaagccgccc ctttgatgag
    11341 gcagcttttt ttaaaataaa ttaagatctt attttacgat ggtcatttcg tcaataaggt
    11401 ttttagcacc ggcatatttg tcgatgacga acaacacata acgaatgtcc acgctgatag
    11461 tgcgttgcac ttcgggttcg aaggcaatgt cgccgctcat ggcttcccag ttgccgtcaa
    11521 aggctatgcc tatcaattgt ccatcaccat tgattacagg acttccagag ttgcctccgg
    11581 taatatccag gttggaaatg aaacaaaccg gcattacacc attattaccg tattggccgt
    11641 aatctttgtt tttataaagt tgtttcaatt tttcgggaac cacaaactca ctattcgaag
    11701 catcttcttt ttccattacg ccatcaaggg tagtaaaata atcgtaatgc accgcatcgg
    11761 cagcttcgta gtcaagcact tttccataag tcatgcgcag ggtagaattg gcatccgggt
    11821 agaatttctt atccggctgc attttccgca aagcctcgct gaacaatcgg gtagccttat
    11881 tcagcttgct ttctatggct ttttgttccc cggcactgtt tctgtaataa ttcaggatac
    11941 tcatggtagt cttacgaacc aaatcattgc tgaatttctt ggcattgggc ttgctgacaa
    12001 aggcgttgaa cgcttcttcc gatgcataaa tggatgtttt aaatgcttca tcagtgaatt
    12061 tctgaatatc gcctttgaaa tttttatcaa tcacatcaaa aatatcggcg cgcatctctt
    12121 tacggaggat ttccaggccg gcggcaaata atttcttttc agtagaagta ttggcgtctt
    12181 tgtaaagatc tttgatggtt tccttatagt cattaaacat ttccggttta gtgtttttat
    12241 tttccaccag agaagacatg cccatagtat ataatggcgc taacaaggtt ttggagctca
    12301 tcaatatcat ctggaaatac cagaagttgg cttccgattt atttgccaat aattgtttgt
    12361 taccctcttc aatatctttt ataacattgc catactttgc tttccggctc tcatcggcat
    12421 caatccactt ttgcagttca ttttccaggg ctttcttttt atcataaact ttcagccgtt
    12481 ttagcccgcg gctttcgcct attttgtttt tccagacatt ggccaaactc gcataggaag
    12541 cggaatattt tattcttaca gcggggtcgg cttccatatc ttttttcata atcggaagca
    12601 taatatttcc tgcattgata atggcagggt tggtttgttc cagggtggtt tgtattccgt
    12661 aagaagtcat atagcgttcg gtagtgcccg gataacccca aatcatggca aaatcacctt
    12721 ttttaatacc cttcagagaa accggcaggt gatgctttgg ttttaaagga atgtttttgt
    12781 cggaatattc agcaggtgag ccatcgggag cggtatatat gcgaagcata gaaaaatcac
    12841 cggtatggcg cggccacatc cagttgtcgg tatcaccgcc aaatttacca atagctgacg
    12901 gaggagcacc aacaaggcgg acatctttat agattatata aacaaacata taatactcgt
    12961 tgttgtcaaa aaaacttttg acatccacaa aatattttcc tttttccgac gcatcttttt
    13021 cgagtttatc ggaaactttt tttatagccg aactgcgggc gctttcgctc atggtatcgt
    13081 ttaccgcagc taaaacagtt gaagtaacat cttccatgcg gacaagaaat gaggccgtaa
    13141 ctcctgtgca tggcaattct tctttcatgt taaaagccca gaaaccattg gtcaggtaat
    13201 ctttttccac cgaactctgg ttctgaatag cgtcaaaacc gcagt
//
LOCUS       DAOSFP010000008        13025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4010_length_13025_cov_4.315189, whole genome shotgun sequence.
ACCESSION   DAOSFP010000008 DAOSFP010000000
VERSION     DAOSFP010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_13025_cov_4.315189"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLI16_00575"
     CDS             complement(<1..518)
                     /locus_tag="PLI16_00575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838560.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOH83082.1"
                     /translation="MAEIKPAEVTAILRQQLAGFKYESELEEVGTVLQVGDGIARVYG
                     LTQVGSGELVEFEKTGVMGVVLNLEEDNVGVVLLGESENIIEGDIVKRTKRIASLNVG
                     EGMLGRVINTLGQPIDGKGPIEGPVYEMPIERKAPGVIFRQPVNQPLQTGIKAIDAMI
                     PIGRGQRELIIG"
     gene            complement(532..1089)
                     /gene="atpH"
                     /locus_tag="PLI16_00580"
     CDS             complement(532..1089)
                     /gene="atpH"
                     /locus_tag="PLI16_00580"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOH83083.1"
                     /translation="MISERIAARYARALFSLALEKAQVEDIYKDMVLLKEVCEKNSDF
                     VHFLGSPIINHGKKVAVFKALFFEKLTPLSFNFLTILIKKRRELIIREIATEFVLLYK
                     EHHNIKTVYFQTASPVSEKNIQQLKDVVGKQLSSKIELIQTVNENLIGGFIFKVDDLQ
                     YDATIRRNINRLKKEYNINIYIPKF"
     gene            complement(1105..1599)
                     /gene="atpF"
                     /locus_tag="PLI16_00585"
     CDS             complement(1105..1599)
                     /gene="atpF"
                     /locus_tag="PLI16_00585"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOH83084.1"
                     /translation="MELVSPGIGLIFWMTLSFLLLMLILGKFAWKPVLKMLKERENKI
                     EDSLKQAEKAHEEMKQLKFSNEELLREAKNERDNILREARVIREKLIEEARTKATEEA
                     DRIVQSAKDSIKYEKLEALTDLKNQIALLSVEIAEKLLQEELSKDENQNKLIEKFLNE
                     INFN"
     gene            complement(1678..1938)
                     /gene="atpE"
                     /locus_tag="PLI16_00590"
     CDS             complement(1678..1938)
                     /gene="atpE"
                     /locus_tag="PLI16_00590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOH83085.1"
                     /translation="MSLMSVLLQAAADYAPIAKMGAGIGAGIATVGAGLGIGNIGKGA
                     MESIGRQPEAVGDIRSNMIVAAALIEGVAFFAIVVCLLILFI"
     gene            complement(1976..3058)
                     /gene="atpB"
                     /locus_tag="PLI16_00595"
     CDS             complement(1976..3058)
                     /gene="atpB"
                     /locus_tag="PLI16_00595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOH83086.1"
                     /translation="MYSNSLKSKRYILVLCCFLSFFISQKAFCETESKPHGQEKKFDP
                     GKTIMDHIADAHEWHIATIGTKHISIPLPVILYDEGKIVAFMSSRFHHGTEAYKGYQL
                     NEKGKIVKVDESAKPPLDFSITKNVVSLFFSVILICWMFISVARRFKNHQNEAPKGLQ
                     SWLEPLILFVRDDIARPSIGEKRYERYMPYLLTVFFFIFINNLLGLIPFFPGGANLTG
                     NISVTMVLALFTFIITTVSANKHYWKEIYNAPGVPWWLKFGLPIMPIVEIIGVVVKPF
                     VLMVRLFANITAGHIIALGFISLIFIFGQISTGAGYGFSVLSVLFYVFMGLLELLVAF
                     IQAYVFTLLSALYFGMAMAEHHEEAH"
     gene            complement(3172..3555)
                     /locus_tag="PLI16_00600"
     CDS             complement(3172..3555)
                     /locus_tag="PLI16_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83087.1"
                     /translation="MPLKKKLLLNTLLFSILLEIIAVLMYFTLDKNIVSPALPFLVPF
                     FFSVSLLSNLWLVSIPPDNPNQFVRTFMLTVFLKFMLYVIVLVVYVMLYRFDAMRFLF
                     TFLILYIIYLVFDVIILLQHLPKKQ"
     gene            complement(3556..3768)
                     /locus_tag="PLI16_00605"
     CDS             complement(3556..3768)
                     /locus_tag="PLI16_00605"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOH83088.1"
                     /translation="MKNLKKPLNNYARYTSIAMQMLVIIVLGVWGGVLLDKYIGIKFP
                     VFTVLLSLGSVSLAIYVAVKDFLKKK"
     gene            complement(3740..4144)
                     /locus_tag="PLI16_00610"
     CDS             complement(3740..4144)
                     /locus_tag="PLI16_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOH83089.1"
                     /translation="MAKNTEVEAPVINILGNGTSIKGDIKASGDMRIDGTLIGTINAR
                     GKVVIGPTGRVDGEIICQSADFSGNLNGKITVTELLAMKASSQILGDIVTGKLSVEPG
                     AKFTGNCKMSETPNNHIPASQEMDEKFKKTAQ"
     gene            complement(4183..6801)
                     /locus_tag="PLI16_00615"
     CDS             complement(4183..6801)
                     /locus_tag="PLI16_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83090.1"
                     /translation="MFKTLLIVFCCFMIFSFSCSTKKNTFTRRVYHNLTAHYNAYFNG
                     KEALKEGVGELGKKCKDDFTEVLPVFQLGTKADAQSVYNLLDRSLEKGSIVVQKHSIY
                     IKGVEHIKWIDDGYMLIGKSYFYKQEYDLAMQTFNFVLNRFKNSSIKYEAVIWKARVQ
                     AQQGNLDDAEALLNSIEKKIEKNKSTRTAERLYPLAYADVLLKQEKYDEALDYLQQAY
                     HLNRSKAIRTRLHFIMAQAFQRTGNGQKATENFKEVIKMNPVYDMEFAAKINLAKSYD
                     VTMGGSKDIRKLLEKMLRDEKNKEYQDQIYYALAELCQKENDIPGMIENLKNSARTSI
                     SNNKQKSLSYLKLADLYFAEPAYREAQIYYDSCIMFLPKDYPNYSLIDNKKNTLNELV
                     RNIDIVELQDSLQMLAKLPAAERNQKIDNLIAEIIKEEQRKIQEENERMNNLALAQQN
                     QNNNMSLQNSGSWYFYNPSTMSLGYTDFVRKWGNRKYEDLWRLSNKAVTDFGFTEVTD
                     ENESNSEKKDTAAVNLKDRNYYLSKLPKTQKDFDTSNLMISEALFNIGYTYKEYLSDY
                     DRSITAFDKLEKRFPESGKILPAYFNLYQIYILQENTPKANYYKNLIMTKYPDSDYAM
                     IINDPNYYIKLQERANRLSVFYKETYLAYGSGNYPAVIRNADSVLKTQKDKNLLPKFA
                     LLKALAVGKTQPQQAFEASLNQVVTKFSGTESAQKAQEILDAIKKKVATQPEVSTDQD
                     TVSAKADPKELYKYEPDAFHFYIAVIEMKAASINEIRNAYSNLNTKYYSTKKYKIDTW
                     FLDDKHEVLNINRFDNKNDAMNYLTALENNKELMETLQKTSYTHFVISAANYPVFYQS
                     KDVDKYLSFFSKNYKK"
     gene            6959..7843
                     /locus_tag="PLI16_00620"
     CDS             6959..7843
                     /locus_tag="PLI16_00620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH83091.1"
                     /translation="MSKDQPSRWKRFRKKLHDKYRLVIMNDSTFERKLSFRLTRMNVF
                     VVVGAAVMMLIGATTLLIAYTPLREFIPGYGDISVQRDVMLLLRKTDSLEKELDLRRA
                     YMENLKYIIEGKDFPSPKMEKPSDSIMIQHNNLSFDISPEDSALRNEFENYDRYNLTF
                     SGGSAESSNISSFFFFSPIKGIVDNGFNPAKKHYGVDILAGKNEAVKATLSGTVIFSG
                     WTVETGYVIGLQHSNNIFSIYKHNSALLKKMGDYVNAGEPIAIVGGTGEISTGPHLHF
                     ELWHNGNPINPKEYMSFK"
     gene            7854..9017
                     /locus_tag="PLI16_00625"
     CDS             7854..9017
                     /locus_tag="PLI16_00625"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOH83092.1"
                     /translation="MKKRIAILGSTGSIGTQALEIIRAFPEKFSAEILTADSNACLLI
                     KQALEFMPDAVVITNDTRYEQVKAALEPHDIKVYAGHESLEQIVESGNIDMVLTAIVG
                     FVGLRPTLNAIRNKKPLALANKETLVVAGDIITRTAREEAVNIYPVDSEHSAIFQCLA
                     GEFHNPVEKIFLTASGGPFRGKDREVLANITKEEALKHPNWVMGNKITIDSATLMNKG
                     LEVIEARWLFNMKPEQIEVLVHPQSIIHSMVEFADGSVKAQMGLPDMRLPILYAFSYP
                     SRMKTDFPKLDWQKIRELTFEPPDTATFHALTLAYEALKTGGNMPCIMNAANEIAVKA
                     FLTDTIKFLAIADVIEQGMQKATYIKNPSLEDYFQSDRETRTITKEIIKKFYK"
     gene            9014..10357
                     /gene="rseP"
                     /locus_tag="PLI16_00630"
     CDS             9014..10357
                     /gene="rseP"
                     /locus_tag="PLI16_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOH83093.1"
                     /translation="MSSLIMIAQLILGLSILVFVHELGHYLAARAFKIRVDKFYLFFD
                     AWNFKFFKFKKGDTEFGMGWLPLGGYCKIAGMIDESMDKEQMKQPPQPWEFRSKPAWQ
                     RLIVMIAGVVMNLIVGVIIFTGVIMHYEKEYLPVSSIDQGIYAYPAGREIGFMTGDKI
                     LKINGKNVERFKDVVTANVLMGSTITVDRNGKNLDVIVPDTMYRNYNKTVRDFFIEAH
                     NFDFKIDSVLDTVINKDKTKKASPAFLAGLKSGDKILAIDSTTVTSYGQFREIIWDSK
                     NQNRTLTVLRATDTLSLSLLVDSTGYIGVVSKHPYKTKAYGFFAAIKYGNKDAMTNLW
                     ANVRGLGKIFAGKENASESLQGPIGIMKIYGGIWDWHRFWLITGLISVVLAFMNILPI
                     PALDGGHVIFTTWELITRRKVPEKILERAQVVGMIILIAIMVFAVFNDVWKHFIR"
     gene            10336..10845
                     /locus_tag="PLI16_00635"
     CDS             10336..10845
                     /locus_tag="PLI16_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOH83094.1"
                     /translation="METLHPLSPPMTDINKDLTGLKTILHRAREFCAYQERCRSEMEQ
                     KFQQWGVDRIRAAKMMEQLEKDNFLNEDRYTETFVRSKFRLKKWGKNKLAAELRIKKI
                     PEDLIVRHLQHIDDAEYHSALMMLAEKKSKELTEKDTFKKNQKIARFLISKGYEPGLV
                     FSALKMRDV"
     gene            10850..12859
                     /locus_tag="PLI16_00640"
     CDS             10850..12859
                     /locus_tag="PLI16_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HOH83095.1"
                     /translation="MKLLSKLISRIKKYTTPEMLKSLYKSALLRKIVLYSVGVPVLLF
                     ILFFLFRNTVLHAVIEKKCQEFKEKYRAEIIIKQAAFKGFAGITLEDISVIPAQRDVL
                     FRSGRIYAHIRPLPLLAGKVRINEVLLENTMINLIRHGKQNNYGFLFKHQKDSTAKVT
                     DSTYNYAERLDRIFSGIFSNIPDDIEIRNFLVHATSDTNSVTAFLPSFHIENYHFLSM
                     VAVSEKQIERVLFVRGEINKSRKRLNFMVYAPHLQKVHVPYIQSKYGFRCDFDTLYAG
                     IAVQGNSSELSINGENLITGLVLNHKKIAQSDVLFKKIALKLNIRASRDYVELDSTSL
                     IAYNRFALNPYIRACHKPRVKFILKINHEFTAQNFFESLPGGMFGNFAGIKTEGKLRL
                     AVNFDIDMRQPDSLRFDATLTGKDFHIIKYGATDFRMINGSFSHTAYVNGLPVRTFIV
                     GPDNPAYTPLEMISPCLKDAVLISENGGFFYGDGFNVAAFRESIIANIHAGRFVRGGS
                     TIDMQLVKNVFLSKNKTIARKAEEILISWLINNNNLCTKEKMYEVYLNLIEWGPGVYG
                     VSEASEYYFQKKPAQLSLPESVFLASIIPKPRWFKSSFDETGKLSTKYQGYFSLIAKK
                     MIDKGGATPQDTLDMIKKVEIKGNSKIFMAKDTTHFKIDSVIMLF"
BASE COUNT         3701 a         2795 c         2725 g         3804 t
ORIGIN      
        1 tcaccgatga taagttcgcg ctggccgcgg ccaataggta tcatggcgtc gatggctttg
       61 ataccggttt gcaatggttg attgacgggt tgcctgaaaa ttactcccgg ggctttgcgt
      121 tcgatgggca tttcatacac gggtccttcg ataggtcctt tgccatcgat aggctgtccc
      181 agggtattga taacccttcc aagcattcct tcacccacgt ttaacgaggc gatacgttta
      241 gttcttttaa cgatatcgcc ctcgatgata ttttccgatt cgcccaaaag tactacgccc
      301 acattgtctt cctcgaggtt gagtaccacg cccataacac ctgttttttc gaactcaacc
      361 aactcgccgg agcctacttg tgtaagaccg taaacacggg caatgccgtc gcctacctgg
      421 agtactgtgc cgacttcttc aagttccgat tcgtatttga acccggccag ttgctggcgc
      481 aatattgcag taacttctgc tggttttatt tctgccattc aaataatttg tttaaaattt
      541 tggtatataa atgtttatat tgtattcttt tttcagccga ttgatatttc ttctgatggt
      601 ggcatcgtac tggaggtcgt ccactttaaa aataaatcct cctataagat tttcattcac
      661 tgtttgaatc aactcaattt ttgaggatag ttgtttcccc actacatctt ttaattgctg
      721 gatatttttc tcagaaaccg gagaggcggt ttgaaaatat accgttttaa tattgtggtg
      781 ttctttgtat aacagcacaa attccgtggc aatttctctg atgatcaact ccctgcgttt
      841 cttaatgagg atggtaagga aattaaagga tagcggagtg agtttttcga aaaataaggc
      901 tttaaaaaca gctactttct taccatgatt aatgataggg cttcccagaa aatgaacaaa
      961 atcgctgttt ttttcgcata cctctttcag aaggaccata tccttataaa tatcctctac
     1021 ctgagctttt tcgagcgcca ggctaaataa ggcccttgca taacgagcgg caattctttc
     1081 ggatatcatc agttctacgg tttcttagtt aaaatttatt tcgttcaaaa acttttcgat
     1141 cagtttgttt tggttttcat cctttgaaag ctcttcctgc aggagttttt cagcgatttc
     1201 aacagataaa agcgcgattt gatttttcag atcggtgagt gcttctaatt tttcatattt
     1261 gatgctgtct tttgctgact gaacaatgcg gtcggcttct tcggtggctt ttgtacgtgc
     1321 ctcttcgata agtttttccc ttattaccct ggcttcacga aggatattgt cgcgttcgtt
     1381 tttagcttct ctcaacagtt cttcgttgct gaattttaac tgtttcattt cttcgtgggc
     1441 tttttcggcc tgtttcagcg aatcctcaat tttgttttcg cgttctttaa gcattttcag
     1501 cacaggtttc caggcaaatt ttcccagaat cagcatcaat agcaaaaaag aaagtgtcat
     1561 ccaaaaaatg aggcctatgc cgggagatac tagttccata atttttaacc tttgagttaa
     1621 ttaaaataac atttttctcc tgtgttgttc aggagttttt tgaactggct aaacctttta
     1681 aatgaaaagt atgagcagac aaaccacgat agcaaaaaaa gccacacctt cgataagagc
     1741 tgcggcaaca atcatgtttg aacggatgtc gcctacggct tcgggttgtc ttccgataga
     1801 ttccatggcg cctttaccaa tatttccaat acccaggcct gctcccacgg tggcaattcc
     1861 tgctccgata cccgcgccca tttttgcgat aggtgcataa tcggctgctg cttgtaataa
     1921 aactgacatt agtgacataa ttttttagtt taagttaatt tatagttttt attttttaat
     1981 gtgcttcttc gtgatgttct gccatggcca taccaaagta caaagccgac agcagggtaa
     2041 aaacataggc ctgtataaaa gcaaccagta attccagcaa gcccatgaaa acataaaaca
     2101 atacagaaag tacagaaaac ccgtagcctg cgccggtgga tatttgtccg aaaataaata
     2161 tcaggctgat aaatccaaga gcgatgatat gtcctgcagt gatattggca aagagacgga
     2221 ccatcaggac aaaaggcttg acaacgacac cgataatttc tacaataggc atgatgggca
     2281 gtccgaactt gagccaccag ggtacgcccg gggcattata gatttctttc cagtaatgtt
     2341 tgttggcact caccgtggtg atgataaaag taaataacgc cagcaccata gttaccgaga
     2401 tattgcctgt gaggttggcc ccgccgggaa aaaacggaat gaggcccagg aggttattga
     2461 taaaaataaa aaagaacacc gtcagcagat agggcatata tctttcgtag cgtttttcgc
     2521 caatggacgg cctggcaatg tcgtcgcgaa cgaacaatat gagcggttcc agccacgact
     2581 gcaggccttt gggggcctca ttctgatgat ttttaaaccg gcgggccacc gagataaaca
     2641 tccagcagat caggatgaca ctaaaaaaca aagaaaccac atttttggta atcgaaaaat
     2701 cgagaggcgg cttggcagat tcatccactt ttacgatttt ccctttttca ttaagctggt
     2761 atcctttata tgcctcggtg ccgtgatgaa accttgacga cataaaagca acaattttgc
     2821 cttcatcgta caggattacc ggcaggggga tgctgatgtg ttttgtacct atggtggcaa
     2881 tgtgccattc gtgggcatcg gcaatatgat ccatgatggt tttgccggga tcgaactttt
     2941 tttcctgtcc gtggggtttt gattcggttt cacaaaatgc tttctgcgaa atgaaaaaag
     3001 acaaaaaaca acacaggaca agaatatatc tttttgattt taagctattt gagtacattg
     3061 ctttgggatt cctttgctat tgagtaaacg gggcaaatat aaacatttta taaaaccgga
     3121 aatttttttt tcttttcatt ttttttgttc atggcctttc ccgctgataa attattgttt
     3181 tttaggaagg tgctgaagaa gaataataac atcaaagacc aggtaaataa tatataatat
     3241 gagaaaggta aacaaaaacc tcatagcatc gaagcggtaa agcatcacat aaaccaccag
     3301 cactataaca tatagcataa atttaaggaa cacggtcagc ataaatgttc tgacaaattg
     3361 gttcggatta tccggtggaa tagaaaccag ccaaagattc gacagcagtg aaacggaaaa
     3421 gaaaaaaggc accagaaaag gcagggctgg cgaaacaatg tttttgtcca gggtgaagta
     3481 catgagcacg gcaatgattt cgaggaggat cgaaaacaag agggtgttga gtaatagttt
     3541 tttctttaac ggcatttatt tcttttttaa aaaatcttta acggcgacat aaattgcgag
     3601 ggacacagag cccaaggaaa gcaatacggt aaaaaccgga aattttattc caatatattt
     3661 gtccagcaat acgcctcccc aaactcccag cacgatgata accaacattt gcatagcaat
     3721 gctggtatag cgtgcataat tattgagcgg tttttttaaa tttttcatcc atttcctgtg
     3781 atgcgggtat gtggttgtta ggggtttcgg acattttgca gtttcctgta aatttggctc
     3841 cgggttctac agacaatttc ccggtaacta tatcgcccag aatctgagat gatgctttca
     3901 tagccagtag ttcggtaaca gtaatttttc cattcagatt tcccgaaaaa tcggcgctct
     3961 gacaaataat ttctccgtcc actctgccgg tgggacctat cactactttt cccctggcat
     4021 taatagtgcc gataagggtt ccgtcgatgc gcatatcgcc gctggctttg atgtcgccct
     4081 tgatactggt tccattgcca agtatattaa tgaccggagc ttccacttct gtatttttag
     4141 ccatgactgt agtttttttt caaatgatta ttaattatgt ttttattttt tataattttt
     4201 cgagaaaaag gaaaggtatt tatcgacgtc cttgctctga taaaacaccg ggtagttagc
     4261 tgctgagata acgaaatgtg tatagctggt tttttgcagg gtttccatta gttctttatt
     4321 attttccaga gccgtgagat aattcatggc gtcattttta ttatcaaaac ggttgatgtt
     4381 cagcacctca tgtttatcat caagaaacca ggtatctatt ttgtattttt ttgttgaata
     4441 atattttgtg ttcaggttgg aatatgcgtt tcggatttcg ttgatgctgg cggctttcat
     4501 ctcgattacc gcaatataaa aatgaaaggc atccggttcg tatttgtata attctttcgg
     4561 gtctgccttt gcgcttaccg tatcctggtc agtgctcact tcgggctgag tggccacctt
     4621 ttttttgata gcgtccagaa tttcctgggc tttttgggcc gattcggtgc cggaaaattt
     4681 tgtgaccacc tgatttaatg acgcttcaaa ggcttgctga ggctgcgttt tcccgacagc
     4741 caacgctttg agcagggcaa atttggggag cagatttttg tctttttgtg ttttcagaac
     4801 gctgtcggca ttgcgtatta ccgcgggata attgcccgaa ccataagcca gataagtttc
     4861 tttataaaaa accgacaggc ggttggcccg ctcctgaagc ttgatataat agtttggatc
     4921 attgataatc atggcataat cactgtcagg atactttgtc attatcaggt ttttgtaata
     4981 attagccttg ggagtatttt cctgcagaat ataaatttgg taaagattaa aataggcagg
     5041 caaaattttc cctgactccg gaaaacgttt ttccagtttg tcaaaggctg tgatagacct
     5101 gtcgtaatcc gacaaatatt ccttataagt atatccgata ttaaacaggg cttcgctgat
     5161 cataaggttg gatgtatcga aatccttttg tgttttaggc aatttcgaca ggtagtagtt
     5221 gcggtctttc aaattcactg ctgcagtatc ttttttctca ctattgctct cgttctcgtc
     5281 ggttacttcg gtaaatccaa aatctgtaac ggctttgttg ctcaacctcc acaagtcttc
     5341 atatttgcgg ttgccccatt tacgcacaaa atcggtataa cccagactca tagttgatgg
     5401 attataaaaa taccaggacc cggaattttg caggctcata ttattatttt gattttgctg
     5461 agccagggca aggttgttca tacgttcgtt ttcttcctga attttgcgtt gttcttcttt
     5521 gataatttcg gcaatgagat tatcaatttt ttggttgcgt tcggctgccg gtaatttagc
     5581 gagcatttgt aaactatcct gcaactccac gatatcgata tttctaacca gttcgttcag
     5641 ggtgttcttt ttattatcga tcagcgaata attgggataa tctttgggca aaaacatgat
     5701 acagctgtcg taatatatct gagcctcgcg gtaagccggt tcggcaaaat acaggtcggc
     5761 aagtttcagg tatgacaagg atttttgttt attgttagaa atgcttgtgc gagctgaatt
     5821 tttaaggttt tcaatcatgc ccggaatatc gttttctttc tggcaaagtt ctgccagcgc
     5881 ataataaatc tgatcctggt attccttgtt tttttcatca cgcagcatct tttccaacag
     5941 tttgcggata tctttgcttc cgcccatggt cacatcgtaa ctttttgcaa ggttaatttt
     6001 agcggcaaat tccatgtcgt acaccggatt cattttaata acttccttga agttctcggt
     6061 agctttttgg ccgtttccgg ttcgctgaaa tgcctgtgcc ataataaaat gcagacgtgt
     6121 gcgtatagct ttggatctgt tcaggtgata ggcttgctgc aggtaatcta gggcttcatc
     6181 atatttttcc tgtttcagca acacgtcagc ataagccaaa ggatacaagc gttcggcggt
     6241 gcgggtgctt ttattttttt ctatcttttt ttcaatggaa ttaagcaggg cttcggcgtc
     6301 gtccagattg ccctgttggg cctgaactct ggctttccag atgacagcct cgtattttat
     6361 gctgctgttt ttaaaacgat tcaacacaaa attaaaagtt tgcattgcca gatcgtattc
     6421 ctgcttgtaa aaataggatt ttccaatcag catatagccg tcgtcaatcc actttatatg
     6481 ttccaccccc ttgatataaa tagagtgttt ctgaaccaca atggagcctt tttccaggga
     6541 ccggtcaagc aggttgtaca ccgactgtgc atcggccttg gtgccaagtt gaaataccgg
     6601 caacacttcg gtaaaatcat ccttacattt tttcccaagc tctcccaccc cttccttgag
     6661 agcttcttta ccattaaaat aagcattgta atgcgcggta agattatgat aaactcttcg
     6721 ggtaaacgta tttttctttg ttgaacaaga aaaagaaaag atcatgaaac aacaaaaaac
     6781 aataagcaga gttttaaaca aagattttga tcgcacgtgt aatagggtta aataaaaata
     6841 acttattttt tacaaaatta ttttattttt tttatttctc cctgattttt tcaagtttat
     6901 ttggcttgta tttcaaaagt aattcaataa tttcggatat ttgcaccctt aattaagtat
     6961 gtcaaaagat caaccatcgc gctggaaacg tttccggaaa aagcttcatg ataagtaccg
     7021 tctggtcatc atgaacgatt ccacttttga acgaaaactc tcgttccggc tgacgcgtat
     7081 gaatgttttt gtggtagttg gcgctgctgt tatgatgctt attggagcca ccaccctttt
     7141 gatagcttat actcccctgc gtgaatttat tccgggatac ggtgacatca gcgtacagcg
     7201 tgacgtgatg ctgctcttac gaaaaactga ctctctggaa aaggaactcg atctcagaag
     7261 ggcttatatg gaaaacctga aatatatcat cgaaggcaaa gattttcctt ccccaaaaat
     7321 ggagaaacct tccgacagca ttatgattca gcacaacaac ctcagttttg atatttctcc
     7381 cgaagattcg gccttacgca atgaatttga aaattacgac cgttacaacc tgactttttc
     7441 cggcggatct gccgaaagca gcaacatcag tagtttcttt tttttcagcc ctatcaaagg
     7501 cattgttgat aacggattta accctgccaa gaaacattac ggggtagata tcctggccgg
     7561 caaaaacgaa gcggtaaagg ccaccctcag cggcactgtc atttttagcg ggtggactgt
     7621 tgaaaccggc tatgttatcg gcttgcagca cagtaataat attttttcga tatacaaaca
     7681 caattccgcc ctgctcaaaa aaatgggcga ttatgttaat gccggagaac ccattgccat
     7741 tgtgggcggc acaggcgaaa tatccaccgg tccgcacctg cattttgaac tgtggcacaa
     7801 cggcaatccc ataaacccta aagaatacat gtcttttaaa taatttcagc ccggtgaaaa
     7861 aaagaattgc catattagga tctaccggtt cgataggaac acaggcgctg gaaatcatcc
     7921 gggcctttcc ggaaaaattt tcagccgaaa tactgaccgc agattccaac gcctgcctgc
     7981 tgataaaaca agccctggag tttatgccgg atgcagtggt tatcaccaac gacacgcggt
     8041 atgaacaggt gaaagcggca ttggaacctc atgacatcaa ggtatatgcc ggccacgaat
     8101 cgctggaaca aattgtggaa tcgggaaata tcgacatggt actgacagcc atcgtgggtt
     8161 ttgtcggcct tcgtcccacc ctgaacgcca tcagaaataa aaaaccgctg gcactggcca
     8221 acaaagaaac actggtggtt gccggcgaca tcattacccg gacagcacgt gaagaagccg
     8281 tgaatattta cccggtagat tctgaacatt ccgccatttt tcaatgcctg gccggcgaat
     8341 ttcacaatcc tgttgaaaaa atctttctga cagcatccgg agggcctttc agaggcaaag
     8401 accgggaagt cttagccaac atcaccaaag aagaagcact gaaacatccc aactgggtga
     8461 tgggaaacaa aatcaccatc gactcggcca ccctgatgaa caaaggcctt gaagttattg
     8521 aagcccggtg gcttttcaat atgaaacccg aacaaataga ggtgctggta cacccgcagt
     8581 cgatcataca ttccatggtt gaatttgccg atggctcggt aaaagcccaa atggggctgc
     8641 ccgatatgcg cctgcccatt ttatacgcct ttagttatcc gtcgaggatg aaaaccgact
     8701 tcccgaaact agactggcaa aaaatcaggg aactaacgtt cgaacctccc gatactgcca
     8761 cttttcatgc cctgacactg gcttacgaag cgttaaaaac gggcggaaac atgccctgca
     8821 ttatgaatgc tgccaacgaa attgccgtga aggctttttt aaccgatact ataaaatttt
     8881 tggctattgc cgacgttatt gaacaaggca tgcaaaaagc cacttacata aaaaaccctt
     8941 cgctggaaga ttattttcaa agcgaccggg aaacaagaac aatcaccaaa gaaataataa
     9001 aaaaatttta taaatgagtt cattaattat gattgcccaa ctgatactgg gcttatccat
     9061 tttggtattc gtacatgagt tgggacatta tctggccgcg cgggctttta aaatccgtgt
     9121 agataagttt tacctttttt tcgatgcatg gaattttaag ttttttaaat tcaaaaaagg
     9181 cgataccgaa tttggaatgg gctggttgcc cctgggcggc tactgtaaaa ttgccggtat
     9241 gatcgatgaa tcgatggata aggaacaaat gaaacagcct ccccaaccct gggagtttcg
     9301 ttccaaaccg gcctggcagc gcctcattgt tatgattgcc ggagtggtca tgaaccttat
     9361 tgttggggtt atcatcttta cgggtgtcat catgcactac gaaaaagaat acctgcccgt
     9421 cagcagcatc gatcagggta tctatgctta tccggcaggc cgcgaaatcg gcttcatgac
     9481 aggagacaaa attttaaaaa tcaacggcaa aaacgtagaa cgtttcaaag acgtggtaac
     9541 ggctaatgtg ctgatgggct ctaccatcac cgttgaccgc aacggaaaaa acctcgacgt
     9601 aattgttccg gacaccatgt acagaaatta caacaaaact gtgagggatt tttttattga
     9661 agcacataat tttgatttca aaattgacag tgtacttgat acagtcatca ataaagataa
     9721 aaccaaaaaa gcaagtcctg cctttttagc cggcctgaaa tcgggggata agattctcgc
     9781 cattgactca accacagtaa cgagttatgg ccagttcagg gaaatcatct gggattcaaa
     9841 aaatcaaaac cgcacattaa cagttctcag ggcaaccgac acgctgtctc taagcctgct
     9901 ggtagattct accggctata tcggtgtggt aagcaaacat ccgtacaaaa ccaaagctta
     9961 tggttttttt gccgccatca aatacggaaa caaagacgcc atgacaaacc tgtgggccaa
    10021 tgtacgcggc ctgggaaaaa tctttgccgg taaggaaaac gccagcgaat cgctgcaagg
    10081 ccctatcggc atcatgaaaa tatatggcgg tatctgggac tggcacagat tctggcttat
    10141 cacagggctg atatccgtgg tgctggcctt tatgaacata ctgccaatcc cggcgctgga
    10201 cggcggtcac gtgattttta ccacctggga actgattacc cgcagaaaag ttcctgaaaa
    10261 aattctggaa cgcgcacagg ttgtcggcat gattatcctc atcgccatca tggtgtttgc
    10321 ggtatttaac gatgtgtgga aacacttcat ccgctaagcc ctccgatgac cgacatcaac
    10381 aaagacctca ccgggctgaa aacaattttg cacagagccc gtgagttttg cgcctaccag
    10441 gaacggtgcc gctccgagat ggaacaaaaa ttccagcagt ggggcgtgga cagaatcagg
    10501 gcggcaaaaa tgatggagca attggaaaaa gacaacttcc tgaatgaaga cagatatacc
    10561 gaaacttttg taagaagcaa attccgttta aaaaaatggg gtaaaaataa acttgccgca
    10621 gaactgcgca taaagaaaat tcccgaagac cttattgtaa ggcatcttca gcacatcgac
    10681 gatgcagaat accattcagc cctgatgatg ctggcagaaa aaaaatcaaa ggaactcaca
    10741 gaaaaagaca cgttcaaaaa aaatcaaaaa atcgccaggt ttcttatttc aaaaggctat
    10801 gaacccgggc tggtattttc ggcactaaaa atgagggatg tctgaaacaa tgaaactctt
    10861 atcaaaactg atatcccgca tcaaaaaata cacaacgcct gaaatgctga aaagcctgta
    10921 caaatcagcc ttgctcagaa aaattgtttt gtattctgtg ggtgtgccgg tattgctttt
    10981 tatcctgttt tttcttttcc gcaataccgt attacatgct gtaatcgaaa aaaaatgcca
    11041 ggagtttaaa gaaaagtacc gtgcagagat tattattaaa caggccgcct ttaaaggttt
    11101 cgccggcatc accctggagg acatatcagt tattccggct cagcgtgatg tgctgttcag
    11161 gagcggcagg atatacgcgc atatcaggcc tttgccgctg ctcgcgggca aggtgcgcat
    11221 caatgaagta ttgcttgaaa acaccatgat caaccttatc cgtcatggca agcaaaacaa
    11281 ttatggcttt ttattcaaac atcaaaaaga ctccaccgca aaagtcaccg attccaccta
    11341 caattatgcc gaacggcttg acaggatttt ttcgggtatc ttttccaaca tccccgatga
    11401 catcgaaata cgtaacttcc tggtacacgc cacgtccgac accaattccg ttacggcttt
    11461 tttgccctca tttcatattg aaaattacca tttcttatcg atggtggctg tttcggaaaa
    11521 acaaatagaa cgtgtgcttt tcgtaagggg agaaataaac aaaagccgga aacgcctgaa
    11581 ttttatggtc tatgcgcccc acctgcaaaa agtacacgtg ccttacatac aatcaaaata
    11641 cggattccgc tgtgatttcg acaccttgta tgcgggcatt gcggtgcagg gcaacagttc
    11701 cgaactcagc ataaacggcg aaaatctgat caccggcctg gtgcttaatc ataaaaaaat
    11761 cgcacagtcg gatgtcttgt tcaaaaaaat tgcgctaaaa ctcaacatca gggccagcag
    11821 agattacgtg gaactcgaca gcacctccct gatcgcttat aaccgttttg cacttaatcc
    11881 atatatcaga gcctgccaca aacccagggt gaaattcata ttgaaaatca atcatgaatt
    11941 tactgctcaa aatttttttg aatcgttacc cggcgggatg ttcggaaatt ttgcaggtat
    12001 taaaaccgag ggcaaactgc ggctggcggt gaattttgat atagatatgc gccagcccga
    12061 ttccctaagg tttgatgcaa ccctcaccgg caaagatttc catataataa aatatggtgc
    12121 gacagatttt cgcatgataa acggatcgtt cagccatacc gcctatgtga acggcctgcc
    12181 tgtaagaaca tttattgtcg gccccgacaa ccccgcatat acgccgcttg agatgatttc
    12241 gccctgcctg aaagatgccg tcctgatttc cgaaaacggc ggtttctttt atggtgatgg
    12301 gttcaatgtg gccgctttcc gcgaatcaat tattgccaac atacatgccg ggcgttttgt
    12361 tcgtggcggg agtactatcg acatgcagct tgtaaaaaat gtcttcctga gcaaaaacaa
    12421 aaccattgca cgcaaggccg aagaaatact gatttcatgg cttatcaaca acaacaatct
    12481 ctgtactaaa gaaaaaatgt acgaagtcta tctgaacctt atcgaatggg ggcctggcgt
    12541 ttatggagtt tccgaagctt cggaatatta tttccagaaa aaacccgcgc agctaagttt
    12601 gcccgaaagt gtattcctgg ccagcatcat cccgaaaccg cgctggttca aatcttcttt
    12661 tgatgaaaca ggaaaactat caaccaaata tcaagggtac ttcagcctga tcgctaagaa
    12721 aatgatagac aagggaggcg ccactcctca ggatacgctg gacatgataa aaaaggttga
    12781 gatcaaaggc aactccaaaa tattcatggc taaggacacc acgcacttta agattgattc
    12841 ggtaataatg ctgttttaac atgccaatag agatcaacaa gttctttgtt catcgcatca
    12901 ataataatcc tgacagtagg ttttttgact ttgaaaaatc catgtgaatt ttccctcatt
    12961 gtttttttta tatttgttgt ttattaatta tgcccattaa tcctacgcgc aacatattat
    13021 ttttt
//
LOCUS       DAOSFP010000009        12997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4022_length_12997_cov_4.564750, whole genome shotgun sequence.
ACCESSION   DAOSFP010000009 DAOSFP010000000
VERSION     DAOSFP010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_12997_cov_4.564750"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="PLI16_00645"
     CDS             complement(<1..715)
                     /locus_tag="PLI16_00645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH83096.1"
                     /translation="MDKSLEYYLKEQNILSETDRQIARKVLSGERITFQEGIHLFENT
                     DTGLLAVMADNVRHRLNGSKVYYNKNVHLEPTNICIYDCQFCSFSRKIGEAGSWNYSY
                     EDVEKIVNNMAEDITEIHITGGTHPEASLAGYELLIKQVRRLRPQVHIKAFSAAELFN
                     VFRKAKIHDDEGLQILIDAGLNSIPGGGAEIFDEAIRKQICPEKLSAAAWLALHKAAH
                     LKGLFSNATMLYGHQELYSH"
     gene            complement(772..3522)
                     /locus_tag="PLI16_00650"
     CDS             complement(772..3522)
                     /locus_tag="PLI16_00650"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH83097.1"
                     /translation="MKKLFLIAFLFFFFPAINYAQINRGGTPVSFSGSVTGDYVEKVL
                     PKPDMKLIQEEDEADAKNGVLRKVGRSLLVDINLNNSGSWETLPNGDRIWRLKIKAEG
                     ALALGVYYNHFWLPAGARLYLYNEDHSRVLGAYTQENNTETGLFATELMAGDAFTIEY
                     FEPSRTKGFSVISISEIAYVYRDFKQRNGTKEFGDSESCEVNINCSEGTNWQDEKKGV
                     ARIFLKVGTSYGWCSGTLLNNERQDCTPYMLTADHCGNGASTADLNQWVFYFNYEATG
                     CPDPSSEPTSNSMTGCTLKAHGGNEGNSGSDFYLVQLSQPPTFNPYYNGWDRNNTPSS
                     SGVSIHHPAGDIKKISTYTAALTSAGWNGSGLQSHWRVVWAATANGHGVTEGGSSGSP
                     IFNGSGQVVGDLTGGGSYCSTPTQPDYYGKFSYSWDQNGTTAATRLKDWLDPDNTGIT
                     TLNGFYCGSGTPVNADFSGTPTTVAVGGTVNFTDLSTGNPTSWSWTFTGGTPATANTQ
                     NPTNIQYNTAGIYPVSLTATNASGSDTETKNNYITVGDNAPIADFVANLTTIPVGGTV
                     NFTDLSAGAPTSWSWTFTGGTPDTSSVQNPTGIQYLAAGTYEVSLTATNAQGSDTETK
                     TAYINVSGTGPGIKPCDTLHFPIPGNMVMYSVYYASGVYGYVSGNNGYSDKAKADVFT
                     AVAPHTKMVGAYFKFGKAKNGSGQDYKIAVHAWDNSGTGGAPGNILFTDSLSCAQIQS
                     DINMNTYTFLPFATPYDVTGVFYLGIMLPTNPGDTIVLLTNKNGESMPGTAWELWQND
                     NWLPYSNIASWSYNLSHAIFPVLCNPVTYSVEENKADDILVYPNPANGIINIDFGSYY
                     HENVAIKVYDLLGNEVKSFNHRGGSTNHLQMDLGGFANGFYVLHINTGEQSLVKKISL
                     LK"
     gene            complement(3529..5199)
                     /locus_tag="PLI16_00655"
     CDS             complement(3529..5199)
                     /locus_tag="PLI16_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83098.1"
                     /translation="MKKALLPLIFLCLGFTGVFSQDNAGGIPRSFFYDNLSADIDYVW
                     VQGPDLMQLAAEDNERAAKSEPYRIGVVIPVDFSLNNAGTWTDLPGEDASIWRLTIKA
                     ADARAIGLEYQSFYLPEGAMLFLYNAGKTQTIGAYTSNNNSEDKFFTHEKIYGDELTL
                     ELYVPNAKKQDVAFHITDLEFFYRPGENQGSKGSEACEVNVNCPEGANWQDEKKGVCL
                     IDIKVGSGLFNCTGSLVNNTSQNCTPYVLLADHCIYNSAYPSVADCNAWKFYFHYEAS
                     TCQGTTPSGIFVKNGCTLKAHDTYGSNHSGSDFCLVQINTPILNTLNPYYNGWDHSGS
                     GSPNGVSIHHPAGDIMKISTYTSALASVTYGAPSSHWQVIWAQTVTNHGVTEQGSSGS
                     PLFNSSGKIVGTLTGGGSFCSTPTQPDYYGKFYNHWDKNGTTSAKRLKDWLDPLNTGA
                     ATIAGKAGSTCPADVGVTKIDNAEKKISIYPSPAQNRIVLSTSSVENKLHDVIIYNVM
                     GLAVKNFPELNISSSETIIDISDIPDGVYYLTAKSGKTSLKGEFVKIK"
     gene            complement(5281..6423)
                     /locus_tag="PLI16_00660"
     CDS             complement(5281..6423)
                     /locus_tag="PLI16_00660"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOH83099.1"
                     /translation="MENFIINNPTCLHFGKGVIGDLSKVLAKYGNRVLLVYGKNSIKD
                     NGIYQKIINQMNIAKANVFEYSGIKSNPVISDVDAAAAVGRKNQVDVIVAVGGGSVID
                     SAKIIGLAIPLQNNAWDIMENKVKPTRSIPLIAVLTLAATGSEMNPFAVVQNDQTKQK
                     PGYFSPFNFPKHSFLDPEYTFSVPADYTAYGIVDLVAHALESFFGKGETSLTDRFVSS
                     IIKEAVEYGPLLLKHLNKYEYREKIMFAATCALNGMTSYGKKNGDWGVHGLGHTLSVL
                     YDVPHGASLSVAYPAWMKYFKNQIGEKLSFLGKEIFNTPDPAATIQAFEMFFKQLGAP
                     TRLGDLGLTGRHKIEIINNMLANKVSGDNFIMEKVDFENIFDLMAN"
     gene            complement(6481..9555)
                     /locus_tag="PLI16_00665"
     CDS             complement(6481..9555)
                     /locus_tag="PLI16_00665"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH83100.1"
                     /translation="MKKFLLLTLLLTPLQAAFAQQSENNYPYRTYFEEAYQANPAVPA
                     GVLEAVAYTNTRIRHVNPQVEEPGCTGIPASYGVMGLVLDGKNYFRENLNTVSALSSI
                     PVQEIINNPRKNILAYASAFQYHMSLPDTNRQLSQKIYNTLVRLSQIPVKDNKIGNDF
                     ALNSQIFDVFVFLNNPVNQAKYHFPKYSFDLSNLFGENNLKIFNSTRLFFAEESIEND
                     NGCRYKSLATKAVCPDYNFANCNWVASPNYSSRNGTAISALVMHTVQGSYAGCISWFQ
                     NTSSQVSTQYVVKSSNGQLCQMVLEADKAWHAGTANPYTIGYEHEGYVDDPQWYTEAM
                     YQSSAALSRDICAAYGIKPIRTFFRETLDDGTQLDDGLHDLGGETACTKIRGHQHYPN
                     QTHTDPGVNWNWDYYYKLMNNTTPVINYTSATGSFYDTGGSGANYSNDERKFWLIQPA
                     GGASSITLTFSSFNVEANWDFLYIYDGTNEFAPLIGRYNTVAPTTITTTGGALFIEWR
                     SDCATTASGWSATWTSVQPDIVAPTTSASVTGTWQTQDFTANFTDTDNAGGSGIEKRF
                     YQVLEFDGNEWHANASRGFFGDNFDSYVSSVWSVPAAGGTWVVNNGYLIQTDSSVNNT
                     NIYAALNQGLSNRYLYDFNVKIDPATSGTNQHRFGLHFYCDNASLSNRGNSYFIFFRQ
                     ETSKLEFYRVENDVFSQMFVINNVVTNFGQWYNIKVIHDRITGKISVYRDNILLGVWT
                     DPNPLTTTANHFSFRTGHSKVYIGEIKVYRSRYPSVTVTVGADPAKDIRFQNPDPATF
                     CAKVKSIVNDAAGNLSAIDYYNINVDWSPPLCAPVNDGTGSDISTTSSLNTLSANWPA
                     STDVNSGISKYWYAIGTSQGATDIVSWTDNALNTSVTRSGLTLTDGQTYYFSIKTVNG
                     AGLQTICSSDGVTVNTTTSVAEQDNDQVARVYPNPFNENTSISFYVSCKQKIRLTITD
                     LLGREILIADEVYAEGEHKININSKKLNLSKGVFILKLFYDKKTDTSLLIKY"
     gene            complement(9988..11757)
                     /gene="sppA"
                     /locus_tag="PLI16_00670"
     CDS             complement(9988..11757)
                     /gene="sppA"
                     /locus_tag="PLI16_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOH83101.1"
                     /translation="MKEFFKMMFASMAGYLLLSLIMFIIFISMMVSLASFSKKTAVEV
                     KQNSVLTLKLDQAIPDRTSENPLGDMDFFSMKSNKVMGLDHLLKTIDNAANNDKIKGI
                     FLDLTDIPSGMATIEEVRNALLQFKKSGKFIISYSDDYTQRAYYLATVADKVYLNPQG
                     CVGIKGLAAELMFFKGTLEKLDIQTQIIRHGKFKSAVEPYILDKMSQANREQYQKLLD
                     VLWQQMVDGIATQRKIATEDLNLMADSLKIQLGEDAVKNKLVDKLLYRDEVLTELRAK
                     LGVNEKDDINFISAAKFFKVKGKEKISIGKKKIALVYAIGQIGVGEGDEREIGSDKLS
                     ESIRLARNDSSVKAIVLRINSPGGSALASEVIWREVVLAQKVKPVVVSMGEVAASGGY
                     YIACGAGKIYAQPNTLTGSIGVFGVVPNLEKFFKNKLGITFDVVKTNEHSDYITATRA
                     MQPYETKVLTNQVENIYKVFVGHVAEGRKMKPEDVDSIGQGRVWTGLDAKRIGLVDEI
                     GGLQNAIDDAAKKANLTDYKVVVYPKFKDPFTQIFEDLMDESSETQLQKTLGENYIYY
                     EHLKNIKTQKGIQARLPFDMVIY"
     gene            11900..12397
                     /gene="folK"
                     /locus_tag="PLI16_00675"
     CDS             11900..12397
                     /gene="folK"
                     /locus_tag="PLI16_00675"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOH83102.1"
                     /translation="MVCFCLGSNVGDRAAYLKNSLIMLEENFGRACKISSVYETEPWG
                     VSKHDDYLNLVAAFETGLSPEKVFRITSSIEKKLGRTRRTGKIDPRTIDIDILFYNDL
                     VINKNDLIIPHPRMASRRFVLQPLTEIMEHFVHPVSGQTMAELFSQCTDNSLVVKTKI
                     NLFLH"
     gene            12397..>12997
                     /locus_tag="PLI16_00680"
     CDS             12397..>12997
                     /locus_tag="PLI16_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOH83103.1"
                     /translation="MYDFIAIEGNIGAGKTSLSTKIAQQFDARLILEQFEDNAFLPKF
                     YRDPEKYAFPLELTFLASRYQQLKEQLTHKDLFQTFTISDYYIYKSLIFAKKTLQDDE
                     LALYTRLFNIINGTLPKPDLLVYLYVEVPRLKRNIITRGREYEQDIEHDYLERIQSGY
                     FDFIKQQTDLRILILDVNKVDFVHCEADYEKILGIIMREY"
BASE COUNT         3430 a         2869 c         2911 g         3787 t
ORIGIN      
        1 ggtggctata cagctcctga tgcccgtaca acatcgtggc gttggaaaaa aggcccttca
       61 ggtgggccgc tttgtgcagg gcaagccagg ctgcagccga tagtttttca ggacatattt
      121 gttttcttat ggcttcatca aaaatttcgg cgccaccgcc gggtatggag tttaaaccgg
      181 catcaatcaa aatttgcaga ccttcgtcat cgtgtatttt tgcttttcgg aaaacattaa
      241 acagttcggc agccgagaac gccttgatat gaacctgtgg ccttaaccgc cgtacctgtt
      301 ttattagcag ctcgtagccg gccaggctgg cttcgggatg cgttcccccg gtgatgtgga
      361 tttcggtgat atcctccgcc atattattga caattttctc cacatcttca taagaatagt
      421 tccaggaccc tgcctcccct attttcctgg aaaaagagca aaattgacaa tcgtaaatac
      481 aaatattggt gggttcaaga tggacatttt tgttgtaata aacctttgag ccatttagcc
      541 tgtgacgcac attatcagcc ataaccgcaa gaaggccggt gtcggtattt tcaaacagat
      601 gtatgccttc ctgaaaggtt attctttcgc cagacaacac cttgcgggca atctgcctgt
      661 cggtttcaga aagtatattt tgctccttaa gataatattc aagggattta tccataccaa
      721 aagctatcga cgtttttgga ttatcagata gctattttat gccaggttac actatttcaa
      781 caagcttatt ttcttcacca gtgactgttc tccggtattt atatgcagca cataaaaacc
      841 attggcaaaa ccgcccagat ccatctgcag gtgattagtg gaaccgccac ggtgattaaa
      901 acttttcact tcattgccca gcaggtcata aacttttatt gcaacattct catgataata
      961 gctgccaaag tcaatattga tgattccgtt agccgggttg ggatacacaa ggatgtcgtc
     1021 cgccttgttt tcctcaacag agtaagttac cgggttacac agcaccggga atatggcatg
     1081 tgagagatta tacgaccagg aagcaatatt tgaataaggc aaccagttat cattctgcca
     1141 gagttcccat gctgtgccgg gcatcgattc accattttta ttggtgagta atactatggt
     1201 gtcgccgggg tttgtgggca acatgatgcc aaggtaaaaa acaccggtaa catcgtatgg
     1261 tgtagcaaaa ggaaggaaag tataggtatt catgttaatg tcgctctgga tctgtgcaca
     1321 ggacagggag tctgtaaaga gtatgttccc gggcgctccg ccggtacctg aattatccca
     1381 ggcatgaacg gcaattttat aatcctgtcc ggagccgttt ttggctttcc cgaatttgaa
     1441 ataagcaccc accattttgg tgtgaggagc taccgctgta aaaacatcgg ccttggcttt
     1501 atcggaatag ccattatttc ctgacacata accatacact cctgaagcat agtacaccga
     1561 atacatgacc atatttccgg gtatcggaaa atgaagcgta tcacagggtt ttattcccgg
     1621 gccggtgccg ctgacgttga tataggccgt tttggtttct gtgtcgcttc cctgtgcgtt
     1681 ggtggcagtc agggaaacct cataggtgcc ggcggcaagg tattgaatgc ctgttggatt
     1741 ctgaaccgag gaagtgtccg gtgtgcctcc ggtaaaagtc caggaccagg aagtgggggc
     1801 gccagccgac aggtcggtaa aatttaccgt accgccaaca ggaatagtag taaggttggc
     1861 aacaaaatca gctattggtg cgttgtcgcc cacggtaata tagttatttt ttgtttcggt
     1921 atcacttccc gaggcgttgg tcgccgttaa ggatacgggg tatattccgg cggtattata
     1981 ttgaatattg gttggatttt gagtattggc cgttgccgga gtaccacccg taaaagtcca
     2041 cgaccaggag gttgggtttc cggttgaaag gtcggtaaaa tttactgttc ctcccacagc
     2101 aacagtggtc ggcgtacccg aaaaatcagc attgacaggt gttccgctgc cacaataaaa
     2161 tccgttcagg gtggttattc ctgtattgtc gggatccagc cagtctttca gccgggtggc
     2221 tgccgtagta ccgttttggt cccatgagta agagaatttt ccataataat cgggctgcgt
     2281 aggtgtagaa caataggacc cgccgccggt aagatcgcca acaacctggc cgctgccgtt
     2341 gaaaatcgga gaccctgacg aaccgccctc ggtaacgcca tggccgttgg ctgtagctgc
     2401 ccacaccacc cgccagtgcg attgtaatcc gctgccattc cagccggcag atgtcagtgc
     2461 ggcggtatat gtggagattt ttttaatgtc cccggccgga tgatggatgc ttaccccgct
     2521 tgaactgggt gtattatttc tgtcccagcc gttataatat gggttaaagg ttggcggctg
     2581 actaagttgc accaggtaaa agtcggagcc ggaattgcct tcgttgccgc catgggcttt
     2641 cagcgtgcat cctgtcatgg agtttgatgt cggttcgctg gatgggtcgg gacagccggt
     2701 ggcttcataa ttaaagtaaa acacccactg gttgaggtcg gcagtactgg cgccgttgcc
     2761 gcaatggtcg gcggttaaca tgtaaggtgt acaatcctgc cgttcgttat tcagcagcgt
     2821 ccccgaacac cagccataag aagttcctac tttcaaaaaa atccgggcca ctcctttttt
     2881 ttcatcctgc cagttggtgc cttcggaaca gttgatattc acttcacaac tttcagagtc
     2941 gccgaattcc ttagtaccgt ttctttgttt aaaatcccgg taaacataag caatttccga
     3001 aatactgatt acggaaaaac ctttagtgcg tgaaggctca aaatattcga tggtaaaagc
     3061 atctcccgcc atcagttcgg tggcaaacaa tcccgtttcg gtattgtttt cctgcgtata
     3121 agcgcccagc accctgctat gatcttcatt atacaggtaa agcctggcac ctgccggaag
     3181 ccagaaatga ttataataaa cacccagggc caaggctcct tcggccttta ttttcagccg
     3241 ccagattctg tcgccgttgg gaagagtttc ccaactaccc gaattgttga ggtttatatc
     3301 aaccaatagg gatcgtccga cttttcttaa aacaccgttt tttgcatcgg cctcatcttc
     3361 ttcctggatt aacttcatat cgggttttgg caaaaccttt tccacatagt ctccggtgac
     3421 ggatcccgaa aaactgacag gtgtgccgcc ccgattgatc tgtgcataat taattgccgg
     3481 aaagaaaaaa aacaaaaaag caattaaaaa cagttttttc atgctgtgtt attttatttt
     3541 tacaaattct cctttaaggg aagtttttcc gcttttggcg gtgaggtaat acacgccatc
     3601 agggatatca gaaatgtcga tgatggtttc tgaagaggaa atgttcaact caggaaagtt
     3661 tttgacggca agccccataa cattgtaaat gataacatca tgcaacttgt tttcgacaga
     3721 ggaggtggaa agtactattc tgttttgtgc aggagaagga tagatactga tctttttttc
     3781 ggcattgtcg atttttgtta ctccaacatc tgcaggacag gttgaacctg cttttccagc
     3841 tatggttgcc gcaccggtat taagcggatc aagccagtct ttcaatcttt tagcgctggt
     3901 ggttccgttt ttatcccaat ggttatagaa ttttccataa taatcgggct gagtgggagt
     3961 gctgcaaaac gatccgccgc cagtgagggt tccgacaatt tttccgctcg aattaaacaa
     4021 gggggatccg gaagacccct gttcggtaac gccgtgatta gtaacagttt gcgcccagat
     4081 tacctgccag tgagaacttg gtgcaccata ggtcaccgac gccagagccg aagtataagt
     4141 ggatattttc attatatcac ccgcaggatg atgtatgctc actccgttag ggctgccgct
     4201 gccgctatga tcccagccgt tataataggg atttagcgtg ttcaggatag gtgtattaat
     4261 ctgtaccagg caaaaatccg aaccagagtg gtttgaacca taggtatcat gagccttgag
     4321 ggtgcatccg tttttgacaa aaatacccga gggggtagtc ccctggcaag tagatgcttc
     4381 ataatgaaaa tagaatttcc aggcattgca gtcagcaaca gaaggatagg cgctgttata
     4441 aatgcagtgg tcggccaaaa gcacatatgg agtacaattc tggctggtat tgttcaccaa
     4501 agaccctgtg cagttgaaaa gtcctgaccc caccttgata tcgatcaaac aaaccccctt
     4561 tttttcatcc tgccagttgg ctccttcggg acaatttaca tttacttcac aagcttcgga
     4621 acctttgcta ccctgattct cgcccgggcg gtaaaaaaac tcaagatccg ttatatgaaa
     4681 ggcgacatct tgtttttttg cattaggaac atatagctcc agggttagtt cgtcgccata
     4741 gattttttca tgggtaaaaa atttgtcctc cgaattattg ttggatgtgt aagcgccaat
     4801 agtctgtgtt tttccggcat tatacaaaaa cagcatagcg ccttccggca ggtaaaaaga
     4861 ctgatactcc aggccaatgg ccctggcgtc ggccgccttg atggtaagcc gccatatact
     4921 ggcatcttcg cccggcaggt cggtccaggt gccggcatta ttcaggctga aatccaccgg
     4981 aatgaccact ccgatcctat agggttcact tttggcggcc cgttcattgt cttcggcggc
     5041 caattgcatc agatcgggac cttgaaccca tacgtaatcg atgtcggcag acaggttgtc
     5101 ataaaagaaa ctgcggggta ttccgcctgc gttatcctgt gaaaataccc ctgtaaaacc
     5161 aaggcaaagg aaaattaatg gaagtagtgc ttttttcatt ttttgtagtt aattgttagc
     5221 tattgatttg aatattgcaa aattagaaaa ttttgtcggc acagctatat gttttggtaa
     5281 ttaatttgcc atcaggtcaa aaatattttc gaaatccact ttttccataa taaaattgtc
     5341 gccgctaacc ttgttggcaa gcatgttatt gattatttca attttatgac ggcccgtgag
     5401 tcccaaatcg cctaaacggg taggagctcc cagttgtttg aaaaacattt cgaaagcttg
     5461 tatagtggcc gccggatcag gagtattaaa tatttctttg cccaaaaagg agagtttttc
     5521 accaatctgg tttttaaagt acttcatcca tgcgggatag gccaccgaca gggaagctcc
     5581 gtggggcaca tcataaagca ccgacaaggt gtggccaagg ccgtgtaccc cccagtcgcc
     5641 gtttttcttc ccataggagg tcatgccatt gagggcacag gtcgctgcaa acattatctt
     5701 ttcgcggtat tcatatttgt taaggtgttt cagcagcaag gggccatatt cgacggcttc
     5761 ctttatgata gaagatacaa aacggtcggt cagcgaagtt tctcccttgc caaaaaaact
     5821 ttccagcgca tgggccacca ggtccacaat accataagcc gtgtagtcgg ccggcaccga
     5881 aaaggtgtat tccggatcga gaaaagaatg tttgggaaaa ttgaatggac tgaaataccc
     5941 aggtttttgt ttggtctgat cattttgaac aacggcaaac ggattcattt cggaacccgt
     6001 ggcggcaagg gtcaacacgg ctatcagggg aatggaacgg gtaggtttta ctttgttttc
     6061 cataatatcc catgcattat tttgaagggg aatggccagg ccaataattt ttgccgaatc
     6121 aataacgctg cctccgccca cagccacaat tacatcaacc tgattttttc tgcccaccgc
     6181 cgccgcggca tccacatcgc tgattaccgg gtttgatttt ataccggaat actcaaaaac
     6241 attggctttg gctatgttca tctggtttat tattttttgg taaataccgt tatccttgat
     6301 ggagtttttc ccataaacca ggagcactcg gttgccatat ttcgcgagta ctttggacaa
     6361 atcgccaatg acaccctttc caaaatgcaa acaggtgggg ttgttaataa taaaattttc
     6421 catagtgtag aatttttgca ggttaaactc ttttttccaa aagaacttta actaatgatt
     6481 ttaatatttg attaacaggc ttgtatctgt ttttttatcg taaaagagtt ttaggataaa
     6541 aaccccttta gacaaattca gtttctttga atttatattg attttatgtt ctccttcagc
     6601 ataaacttca tcggcaatca gaatttctct gcccaacagg tcggtgatag tcagtcttat
     6661 cttttgtttg cagctcacgt aaaaagaaat gcttgtattt tcgttaaagg ggttggggta
     6721 taccctggca acctggtcat tgtcctgttc ggctacggag gtggttgtat tgactgttac
     6781 tccgtcggag gagcatatgg tttgcaaacc ggcgccgttg acggtcttta tgctgaagta
     6841 ataggtctga ccgtctgtaa gagtcagtcc tgatctggtt accgatgtgt tcagggcatt
     6901 gtcggtccag gagacaatat cggtggcgcc ttgcgatgtt cctatggcgt accagtattt
     6961 cgaaataccg gagttgacat cggttgatgc cggccagttg gccgatagcg tgttaagaga
     7021 agatgtggtg ctgatgtcgc tgcctgtgcc atcatttaca ggagcacata agggaggaga
     7081 ccaatccaca ttgatgttgt agtagtcaat cgcagagaga ttacccgcgg catcgttaac
     7141 aatggatttt actttagcgc aaaaagtagc ggggtcggga ttttgaaaac gaatgtcttt
     7201 agcagggtct gcgcccacag taactgtaac ggaaggatat ctcgacctgt aaacttttat
     7261 ttcgccgatg tacactttgc tgtgcccggt acggaacgaa aagtggtttg ccgtcgttgt
     7321 cagcggattg gggtcagtcc atacccccaa aagaatgtta tcgcgataca ccgaaatttt
     7381 tcccgttatc ctatcatgaa tgaccttgat attataccat tgtccaaaat ttgtaaccac
     7441 attatttatg acaaacatct gggaaaacac atcgttttct acccgataaa actctagttt
     7501 tgaagtttcc tgcctgaaaa agataaagta ggaatttccc ctgttgctaa gacttgcgtt
     7561 atcgcaataa aaatgcaggc caaaccggtg ttggtttgtt ccgctggtcg ccggatcaat
     7621 tttcacattg aaatcataca aatacctgtt ggaaagcccc tgatttaatg ctgcataaat
     7681 attggtgttg ttcaccgaag agtcggtctg gataaggtat ccgttattga caacccatgt
     7741 gccgcccgct gccggaaccg accacacgga agacacatag ctgtcaaaat tatccccaaa
     7801 gaaacctctt gaggcattgg catgccattc gttgccatca aattcgagca cctggtaaaa
     7861 ccttttttct ataccggatc cgccggcatt atccgtatcg gtgaagttgg ccgtaaaatc
     7921 ctgtgtttgc cacgtccccg tgacactggc gctggttgtc ggggcaacaa tatcgggttg
     7981 taccgatgtc caggtagctg accaaccgga cgctgttgtt gcgcaatcag aacgccattc
     8041 gataaacagg gctcctccgg tggttgtgat ggttgtgggt gccacagtat tgtatctgcc
     8101 gattaagggt gcaaattcgt tggtgccatc ataaatatag agaaaatccc aattggcctc
     8161 tacattaaaa gaggagaatg tcagggtgat gctggaagcc cctcctgccg gctgaataag
     8221 ccagaatttt ctttcgtcat tggagtaatt ggcgccactt ccgccggtat cataaaaaga
     8281 acccgtagct gacgtatagt tgataaccgg cgtagtatta ttcatgagtt tgtaataata
     8341 atcccagttc cagtttacac cgggatcggt atgtgtctgg ttggggtaat gctgatgtcc
     8401 ccttatctta gtacaggccg tttcgccacc caaatcgtgc agaccatcat caagctgggt
     8461 accgtcatcc agtgtttcgc ggaaaaatgt tctgatcggc ttaatgccat aagcagcaca
     8521 aatatccctg gacaatgcgg cagagctctg atacatggct tcggtgtacc attgcggatc
     8581 atccacatat ccttcatgtt cataacctat tgtataggga tttgcagttc ccgcatgcca
     8641 ggccttgtct gcctcaagaa ccatctggca aagctggccg ttggaagatt ttaccacata
     8701 ttgtgtggag acctgcgagg acgtgttttg gaaccagctt atacagccgg cataactccc
     8761 ctgcacggtg tgcataacaa gggccgagat ggctgtaccg tttcttgaac tataattggg
     8821 tgacgccacc cagttacaat tagcaaaatt ataatcggga cagacagctt ttgtcgccaa
     8881 tgacttataa cggcagccat tatcgttttc aatggattcc tcggcaaaaa acaaacgcgt
     8941 gctgttaaaa attttaagat tattttctcc gaataaatta ctcaggtcaa aagaatattt
     9001 tggaaaatgg tatttcgcct gatttacagg attattcaaa aaaacaaaaa catcaaaaat
     9061 ctgggagttt aaagcaaaat catttcctat tttattgtct ttaacaggaa tttggctaag
     9121 cctcaccagc gtgttataga ttttttgtga caattgcctg ttggtatccg gcaaactcat
     9181 atgatactga aatgccgaag cataggcaag aatgttttta cggggattgt tgataatctc
     9241 ctgaaccgga atggatgaaa gcgcagatac cgtgtttaag ttttcgcgaa aatagttttt
     9301 gccatcaagc accaggccca taacgccgta tgaggccgga atgcctgtac agcccggttc
     9361 ttccacctgc gggttgacgt ggcgtatgcg cgtgttggta taggccacag cttccagtac
     9421 tccggcagga accgcagggt ttgcctgata ggcctcttca aaatacgtcc ggtagggata
     9481 attattttca gattgttgcg caaaagcggc ctgcagagga gtcaataata aggtcagtag
     9541 taaaaatttc ttcatgaatc atactttttg acaaatataa aaataaacac tgaaaaaaat
     9601 gcagagattt tagtttttac cccatccggt ctttaggaga tattcagtaa cactttcgga
     9661 ttccaatcag tataatatag catcatatat tgtttgctgc acgttttggc gaatccggtt
     9721 gtgcgaagtt tccgaacttc acatcgggta aatgttagtc ttcgactaac agaaaaatta
     9781 tggaagaatt attacaaaaa aaatcatact attgtaaatc tatttcagga ttaagtataa
     9841 atctgtaaac ttattttagg acgagacatt atgcagagta cttttgaaac tgcctctcat
     9901 atcaaaaatt aaaaaaggct cttacatcag gtttatgcat ttctggccaa accttttgta
     9961 agagcctttt taggcgaacc gctacggtta atatatcacc atatcgaaag gcagacgggc
    10021 ttgtatgcct ttctgggttt taatgttttt caggtgttcg taatagatat agttttcgcc
    10081 cagcgttttt tggagttgtg tttctgatga ttcatccata aggtcctcaa agatctgtgt
    10141 gaagggatct ttaaatttcg gatataccac cactttataa tcggtaagat ttgccttttt
    10201 tgcagcgtca tcaatagcat tctgcaggcc gccgatttcg tccaccaggc cgatacgttt
    10261 tgcgtcaagc cctgtccaca cgcggccctg gccaatagag tccacatctt cgggtttcat
    10321 tttacggcct tcggcaacat ggcccacgaa aactttataa atattttcca cctggttggt
    10381 caacactttt gtttcatatg gctgcatagc gcgggttgcc gtaatataat cggaatgttc
    10441 attggttttc accacatcaa aggtgattcc gagtttgttt ttaaagaatt tttcaaggtt
    10501 gggtaccacg ccgaaaacac ctattgatcc agtaagggtg ttgggctgag catatatttt
    10561 tccggctccg caggcaatgt aataaccacc cgatgcggcc acttcaccca tagaaactac
    10621 cacgggttta actttttgcg ccaaaaccac ttcgcgccag atgacttctg aggccagagc
    10681 actgcctccc ggagaattta tacgcaatac aatagctttt accgaactgt cgttgcgggc
    10741 aagccttata gattcggata atttgtcaga accaatttct ctttcatcgc cttcgccaac
    10801 ccctatctgg ccgatagcgt acaccagggc aatcttcttt ttgcctatag aaattttttc
    10861 cttgcctttt accttaaaaa acttggcggc gctaataaaa ttaatatcat ctttttcgtt
    10921 aacccccagt ttggcacgta actcggtcag cacctcgtcg cgatacaaca gcttgtctac
    10981 cagcttgttc tttacagcat cctcccccaa ctgtatttta agactgtcgg ccatcaggtt
    11041 aaggtcttct gtggcaattt tgcgctgggt agcaatgcca tcgaccattt gctgccataa
    11101 tacgtcgagc agtttttggt actgttcgcg gttggcctgg ctcattttat ccagtatgta
    11161 aggttccacg gcgcttttaa attttccgtg acggataatc tgtgtctgga tatcaagttt
    11221 ttcgagagtt cctttaaaga acatcagctc ggcggccagg ccttttattc ccacacatcc
    11281 ctgcggattt agataaacct tgtcggctac cgtggccagg taatatgccc tttgagtata
    11341 atcatcactg tagctgatga tgaattttcc tgattttttg aattgcagca gcgcatttct
    11401 cacctcctca atggttgcca taccggaagg aatatcggtc aggtccagaa aaattccttt
    11461 tattttatcg ttatttgcag cattgtcaat ggttttaaga agatgatcga gccccatgac
    11521 cttattactt ttcatggaga aaaaatccat atctcccaga ggattttcgg aagtgcggtc
    11581 gggaatggcc tggtctaatt tcagcgtcag tactgaattt tgttttactt ccaccgcggt
    11641 ttttttgctg aaggaagcca gcgaaaccat catggaaata aaaatgataa acataatcag
    11701 cgacaacagg aggtatccgg ccatcgacgc aaacatcatt ttaaaaaact ctttcatagc
    11761 ttattgtgtt tttggtttat ttttttgtaa aattagttaa taaaaaacat tttttggaca
    11821 tataaaccga acatttgtta caattcggtt tttttctttt gtgattttta tacttttgat
    11881 ttttcaaata cacattaaaa tggtttgttt ttgtctgggg agtaatgtag gcgacagggc
    11941 ggcatatttg aaaaattcgc tcatcatgtt ggaagaaaat ttcggcaggg catgcaaaat
    12001 ttcgtctgtt tatgaaaccg aaccatgggg tgtaagcaaa cacgacgatt acctgaatct
    12061 ggtggctgcc tttgaaacgg gactctctcc cgaaaaagtg ttcaggatta cttcctccat
    12121 tgaaaaaaaa ctcggacgta caagacgaac cggaaaaatt gatccccgta ccatcgacat
    12181 cgacattctg ttttacaacg acctggtgat taacaaaaac gacctgatta ttccgcatcc
    12241 ccggatggca tcacggagat ttgtgctaca gccattgact gagattatgg agcattttgt
    12301 tcatcccgta tccgggcaaa ccatggcgga actattttct caatgtacag ataattctct
    12361 tgtggttaaa acaaaaataa atttattttt gcattgatgt atgattttat agccatagaa
    12421 ggaaatatcg gtgccggcaa aacctccttg tcaaccaaaa tagcccaaca gtttgatgca
    12481 cggctcattc tggaacaatt tgaagacaat gcgtttcttc caaaatttta ccgcgatccc
    12541 gaaaaatatg cctttccttt ggaactgaca ttcctggctt cgcgatacca gcaactcaaa
    12601 gaacagttga cgcataaaga cctgtttcaa acatttacca tttcggatta ttatatttat
    12661 aaatcgctga tttttgcaaa aaaaacgctg caggatgatg agctggcttt gtacacgcgc
    12721 ctttttaaca tcattaacgg caccttgccc aagcccgacc ttctggttta tctttacgta
    12781 gaggttccac gtctcaagcg aaacataata acgcggggca gagaatatga gcaagacatt
    12841 gagcacgatt acctggaacg catacaaagc gggtattttg attttatcaa acagcagacc
    12901 gatctgcgta tcctgattct tgatgtgaat aaggtggatt ttgtacactg cgaagccgat
    12961 tatgaaaaaa ttctcgggat tattatgaga gaatacc
//
LOCUS       DAOSFP010000010        12754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4120_length_12754_cov_5.130089, whole genome shotgun sequence.
ACCESSION   DAOSFP010000010 DAOSFP010000000
VERSION     DAOSFP010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4120_length_12754_cov_5.130089"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2896
                     /locus_tag="PLI16_00685"
     CDS             <1..2896
                     /locus_tag="PLI16_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83104.1"
                     /translation="TSDANGLASWGNINFTETDPEVGTNTTNYLSKWNGTSLVTSSVY
                     DNGNVGIGTTITDARLQIKPTAEAGYTLRLLSQSYDDRYVSIWRGTDGGVIDVVGPAV
                     NPPVHLGFYIEGSEKVRITDAGNVGIGTTTPQGLLHVSSSGYALLKVGKHALLSGLAN
                     NSTLDTAGSVIITGGSDISGIGKGAGSVTVTAGAWGGTDRAKIVLNSVGIGGTGGGVL
                     ISGGFNTAGSISVGRRIYNGSPAGGVVISGGEGGSNGNGGSVTLAAGAGSGDVSYRGG
                     DVIIKGGAGLLPSPAGNILFKIADVSVAKIDSLGRIGIGTTHPGAKLEVNGTVKITDG
                     TQGAGKVLTSDANGLASWGSISITETDPEVGTNTTNYLSKWDGSALITSSVYDNGNVG
                     IGSTSPGQRLDVQGGNINTSGNIMTGSLTRLDGAGNLINIGDITAIGASTYTSGAGAT
                     LALRGGNSSTAEGGAVAIIAGVGGSGAAGSNVTINAADASGIATGGKVMLTGGTGGYY
                     GGSGAYVSINGGAPNYGPGGNLILSGGLQGPGNTWDPNRNGSVILAINGTEYVRVDGY
                     RDGYQGYVGIGTATPAAKLDINGTIKITDGTQGVGKVLTSDANGLASWGSISLIETDP
                     EVGSNTTNYLSKWDGSALITSSVYDNGNVGIGTTITDAKVQIKPTAEAGFTLRLLSHS
                     YDDRYVSIWRGTDGGVIDVVGPAVNPPVHLAFYIDGTEKARITSAGRLGIGTTGPVET
                     LDITGNMKLEGDNTVIYGNKTGASGLGYSFATKLISASQEKAKIEYLQEGAYSTNINF
                     WTRLGTGGFSSQYTEKMRITPSGNVGIGLTNPSARLDVNGEIKATGGNSSEWNTAYGW
                     GNHATAGYEPQIATGTSTQYWRGDKTWQTLDIPPIREVADEFTASVSQTGFTLTQSPS
                     ANSKVKMYINGVRISNSAYTVTGSALTYIPANNGSYSLVAGDRIQFDYYY"
     gene            2914..3840
                     /locus_tag="PLI16_00690"
     CDS             2914..3840
                     /locus_tag="PLI16_00690"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOH83105.1"
                     /translation="MKIFLIIFFCVVTPFGISAQGITQYGGITNSSTDFINKNGATVN
                     VSELGTTGQIIVIASISTVSVSLITSTGALCGGNITNNGGALVTARGVCWNTSANPTV
                     ENNKTLDGTGGGVFTSALSGLTANTTYFARAYATTSAGTGYGNQISFTTTISVGDSYQ
                     GGIVGYILQSGDPGYIPGQIHGIIAATSDQNSTTWWGCPIYGETTGTAIGTGATNTTT
                     IIADPCASPEMAARLCSDFVSGGYNDWFLPSKDELNKLYINRAVIGGFNTFRYWSSSN
                     NGMGAWEQNFSNGSQAQVDRHTQNYVRPVRAF"
     gene            complement(3850..4278)
                     /locus_tag="PLI16_00695"
     CDS             complement(3850..4278)
                     /locus_tag="PLI16_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH83106.1"
                     /translation="MVRITSFSFSDQDLFKEAFTIRQAVFVAEQGVPAELEYDEHEAE
                     ATHYLLYDDETPVGAARWRKTAQGIKLERFAVLPEYRNKGIGGTLVKKVLEDVISLGL
                     PIYLHSQLKACTLYQRHGFVVSGEVFFEAGIAHYKMVLKK"
     gene            4396..4971
                     /locus_tag="PLI16_00700"
     CDS             4396..4971
                     /locus_tag="PLI16_00700"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOH83107.1"
                     /translation="MFELPQLPYAANALEPFISEKTIGFHYGKHHQAYVTNLNKLVAG
                     TEFENATLEEIIKKTTGGIFNNAAQVWNHTFYWNSLAPKPKAQPEGALAEAINNKFGS
                     FDSFKEKFSAACVTLFGSGWAWLVKTPDGSLDIVQESNAGNPLCNGLIPILTCDVWEH
                     AYYLDFQNRRPDYVAEFWKYVNWEEIEKRFL"
     gene            5235..5618
                     /locus_tag="PLI16_00705"
     CDS             5235..5618
                     /locus_tag="PLI16_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006846867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOH83108.1"
                     /translation="MSLEENKISYIARGCIFEVYNQLGPGLLESVYESALSYELLNQG
                     LKIETQVPIPVNYKDIPLEIGFRADIIIENKVIIEIKSIENIAEVHHKQVLTYMKLTG
                     IKLGLLVNFNTEDIGKSIYRKVNKL"
     assembly_gap    5672..5771
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5842..6807
                     /locus_tag="PLI16_00710"
     CDS             5842..6807
                     /locus_tag="PLI16_00710"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOH83109.1"
                     /translation="MNTELKNAAIIALRDCMGLKPEETLLVITDELKRDIGAALHEAG
                     KELCKESLLVEIKSREINGQEPPKQIADLMKMVDAVICPTAKSLTHTNARREASKLGV
                     RVGTMPGITVDCMVRCLNADIQKILDLTDYVVKRMTGIKQIRVVTEKGTDITMPVEGR
                     NVLPSKGVMLNKGESGNLPSGEVYLAPWEGKTNGTVVVDGSMAGIGMLKTPITIEVVD
                     GYAEKITGGEEAVRLTEMLDKVGRDARAVAEFGIGTNYKAQLTGMILEDEKVFGTIHV
                     AFGNNISMGGNISASSHLDGLVKDPDVYFDDIMIMKKGKMLDFEV"
     gene            6907..7350
                     /locus_tag="PLI16_00715"
     CDS             6907..7350
                     /locus_tag="PLI16_00715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH83110.1"
                     /translation="MLKAIIKTVKGDMTVSFYEKDAPGTVKNFVDLAKKGFYNGLTFH
                     RVIPDFVIQGGCPYGTGVGGPGYTIKCETNGGNQYHDRGVLSMAHAGRDTGGSQFFIC
                     HSRQNTAHLDRKHTCFGKVIEGLEVIDAICQGDKINSIEILEEKD"
     gene            complement(7351..9042)
                     /locus_tag="PLI16_00720"
     CDS             complement(7351..9042)
                     /locus_tag="PLI16_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOH83111.1"
                     /translation="MKRKLLIRDLTLRDGQQSLFATRMNQEQVDKVIPLYKEANFYAM
                     EVWGGAVPDSIMRYLGENPWDRLEKIKVAIGDSCKLTALSRGRNLFGYNPYPEEVIEG
                     FCRYAIQSGIGIMRIFDALNDVDNIKSSIKYVKENGGIADATVCYTVDPHFSSKDRIK
                     ALLRAKLLPNNIFTDEYFLNKALEMEALGADMISVKDMAGLIPPVRSGRLIKLLKEKL
                     KIPVDFHTHCTPGYGLASSLMAVINGVDILDTSIMTFAGGPAAPAYELIQLFCNKLGV
                     ETGVNQEVVVKINNILRDIRTEMADVDSYKMFPREFDITRDKLPQDIDDLFELAIAYA
                     KSNKEEELLDCCHKIERYFKFPEPNENVKFAEIPGGMYTNMLAQLKTLKLEKLLPRTL
                     EIIPTVRLAAGCPPLVTPTSQIVGVQAVNCIIDENKGQPFYTTKSIQFVNLVKGQYGH
                     TPIPIDPEFRYKIAGVKEETPYDTKNYRKQDNPVFPEYGDVKLADNPKDELLLELFPA
                     VATDFLKNRVINKYLAEIHRIEEEKLQKHLKEKQAYEDLSPEEKEKRLMEGLNKF"
     gene            9268..10920
                     /locus_tag="PLI16_00725"
     CDS             9268..10920
                     /locus_tag="PLI16_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOH83112.1"
                     /translation="MKKTIVLFCLIAFASFIMQGCGGSKKNDKVMTKEDSIMQARVNE
                     YATVKLTCDLSKLSVKEKQLVAALIDIAAIMDDLYWKQTLGDKQAFLDSIKNENEKKF
                     AMINYGPWDRLDDNKPFMAAYGEKPLGANFYPKDITKEEFEKFDNKDKTGLYTLIRRN
                     EDKSLKVVWYHDEYKEELQKASDLLKKAAGLAEDDGLKKYLELRAEALITSNYQPSDM
                     AWMDMKNNRIDFVVGPIENYEDALYEYKTAFESFVLIKDMDWSKKLDKYIGMLPDIQK
                     QLPVEEKYKKEVPGTSSDLGVYDAVYYAGDCNAGSKTIAINLPNDEQVQLAKGSRRLQ
                     LKNTMKAKFDNIMMPIAQKLTDPAQIKNVKFDAFFNNVMFHEVAHGLGIKNTINGKGT
                     VREALKDEYSALEEAKADILGLFIVTHLIGKGEIKEITVDDCFVTFMAGIFRSVRFGA
                     ASAHGKANMMCFNFFEKAGAFTRNEKGFYTVDVAKMNEAMNQWASTILVFQGNGDYDG
                     VASYRKENATINPQLQKELDGLKSANIPKDVVFEQGKNILGL"
     gene            10983..12407
                     /locus_tag="PLI16_00730"
     CDS             10983..12407
                     /locus_tag="PLI16_00730"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH83113.1"
                     /translation="MRKVLWLGLFLLITGMPDVFSQAYQLPDAAAVSRIKDNIGILAA
                     DSMMGRESGTPGEYMARKYIAAQFKEIGLAPLFDTSYFESFTFSDVDFLDLDNTMEVN
                     GKKLQLYSDYYPIGFSANDTVSGEMVFAGTGILCESAGINDYIGLDLKGKIAVIDLAV
                     PGTLLEKKNVWDSSQKVVRVNRAMSLGAVGVVFVSTEKNYGTPSKDPNFYKERVKIPV
                     VFVCDSSIIMPENPGKAFIITSIDRSGLRRAYNVGGFIDNGSENTVVIGAHYDHVGLG
                     YFGARDPGDNTVHNGADDNASGVAGVLELARMLKSAGPKSNNYAFLAFSGEEKGLIGS
                     EKFITNGSVKKERINYMVDLDMIGRLDAKKKIKIYGTGTADLWENALNTIGDKGFKIT
                     RIKTGIGGSDHYSFNQHQIPAIFIHTGLHEDYHKAVDDTHLINFKGAQEVIKYAYEII
                     RYLDDKGKITFTIASMLDEMMLKK"
     gene            complement(12411..>12754)
                     /locus_tag="PLI16_00735"
     CDS             complement(12411..>12754)
                     /locus_tag="PLI16_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83114.1"
                     /translation="VVMHLPVFSSGPHNTQNKKLRKNLVPLFERYGVDIVFSAHNHCY
                     EKAVSNGIYYITTAGAGAPFYKKAKDLPESQFFVNAHHFCTLKISGNTLRMEAIDTNF
                     RLLDKVEISMP"
BASE COUNT         3907 a         2517 c         2984 g         3246 t
ORIGIN      
        1 tacttctgat gctaatggcc tggcaagctg gggcaacatc aattttactg aaacagaccc
       61 tgaagttgga acaaatacca ccaattatct ttcaaaatgg aatgggacat cattggttac
      121 ttcatcagtt tatgacaatg gaaatgttgg tatcgggact actattactg atgcccggct
      181 tcagataaaa cccactgccg aagcgggata tacattaaga ctgttaagcc agagttatga
      241 tgaccgctat gtttccatct ggagaggtac cgatggcggt gtcatagatg ttgtcggccc
      301 ggcagttaat ccccccgtac atcttggatt ttatatcgaa ggttctgaaa aagtcaggat
      361 aaccgatgcc gggaatgtag gaatagggac gaccactcct cagggccttc ttcatgtgag
      421 tagtagcggc tacgccctgc tgaaagtcgg caaacatgca ttactgtccg gattagccaa
      481 caattcgact ctcgacacgg ccggatcagt aataataaca ggaggtagcg atatatcagg
      541 aattgggaaa ggcgccggat cagtaaccgt gacagccggg gcctggggcg gaaccgaccg
      601 ggcgaagatc gttcttaata gcgttggaat aggcggaacc ggcggaggtg tcttaatatc
      661 cggaggtttc aatacagccg gttctatttc agtgggacgc cgcatttata acggatcacc
      721 tgccggcggt gttgttattt caggaggaga agggggatcg aatggtaatg gcggatcggt
      781 taccctggca gcaggagccg gctccggtga tgtttcatac cggggtgggg atgtcattat
      841 taaaggtggt gcaggccttt tgccctcgcc ggcaggaaac atacttttca agattgccga
      901 cgttagtgtg gcaaagattg acagtttagg cagaataggc attggaacga cacatcctgg
      961 cgcaaagctt gaggtgaacg gaaccgtaaa aatcacagac ggcacacagg gcgccggaaa
     1021 agtgcttact tctgatgcta atggcctggc aagctggggg agcatcagta ttactgaaac
     1081 agaccctgaa gttggaacaa ataccaccaa ttatctttcc aaatgggatg gatcagcttt
     1141 gatcacgtcc tctgtatatg ataatggcaa tgtaggtata ggttccacaa gcccgggaca
     1201 aagactggat gtccagggtg gcaacatcaa tacttcaggc aatataatga caggaagcct
     1261 gacgcgttta gacggggcgg ggaatcttat aaatattggc gacatcacgg ctataggggc
     1321 atcaacatat acatcagggg caggagccac tcttgctttg cgcggaggaa acagcagcac
     1381 tgcagaagga ggtgctgtgg caataattgc aggtgttggt ggttccggag cagcaggttc
     1441 aaacgtgaca atcaatgcgg cagatgccag tggaattgca acaggaggaa aggtaatgtt
     1501 gactggtggt actggaggat attatggtgg aagtggtgcg tatgtttcaa ttaacggagg
     1561 cgctcccaac tatggcccag gtggcaacct gattcttagc ggaggattac agggacctgg
     1621 taatacctgg gatcctaaca gaaacggttc cgtaatttta gcgataaacg gaacagaata
     1681 cgtgcgcgta gatgggtaca gagacgggta tcaaggttat gttggcattg gaacagcaac
     1741 acctgctgca aaactggata ttaacggtac aataaaaatt acagacggta cacaaggggt
     1801 cggaaaagtt cttacttctg atgccaacgg cctcgcaagc tggggtagca tcagtcttat
     1861 tgaaacagac ccggaagttg gatcaaatac caccaattat ctttccaaat gggatggatc
     1921 tgccctgatc acttcttcag tttatgataa tgggaatgtg ggaattggga caaccattac
     1981 cgatgcgaag gttcagataa aacccacggc tgaagcagga tttacgttgc gtttattaag
     2041 ccatagctat gatgatcgtt atgtttccat ttggagagga accgatggcg gtgtgattga
     2101 tgtcgttggc cctgccgtaa atcctccggt acatctggct ttttacattg atggaactga
     2161 aaaggccagg attaccagtg cgggaaggct tggtattggc acaaccggtc ccgttgaaac
     2221 attagatata acaggaaaca tgaaattaga aggagataat acggtaattt atggtaacaa
     2281 aacaggagcc agcggtctgg gatatagctt tgctacaaaa ttaatttctg ccagtcaaga
     2341 aaaggctaag attgaatact tacaggaggg tgcatattca accaatatta atttctggac
     2401 cagactgggg acgggcggtt tttcaagcca gtatactgaa aaaatgagaa ttactccctc
     2461 gggaaatgtc ggtatagggt tgacaaaccc atctgccaga ctggatgtta atggagaaat
     2521 taaggcgaca ggaggaaact cttcggaatg gaatacggcc tatggatggg gaaatcatgc
     2581 cacggctggt tatgaacctc agatagctac gggaacttcc acacaatact ggagaggaga
     2641 caaaacctgg caaacccttg atattcctcc catcagagag gtggccgatg aattcactgc
     2701 ttcagtatca caaaccggtt ttactttaac acaatcccct tcggcaaata gtaaggtaaa
     2761 gatgtatatc aacggcgtga gaatcagtaa ttcggcctat acagtgactg gcagtgcact
     2821 aacctatatt cctgccaaca acggatcata ttcattagtt gcaggggaca gaattcaatt
     2881 tgattattat tattaaaaat ggctaataaa ttaatgaaaa tatttctgat tatatttttt
     2941 tgcgtcgtaa cccctttcgg tatatcagcg caaggtataa cgcaatatgg cggaattacc
     3001 aattccagca ctgattttat aaataaaaac ggagctacag taaatgtttc tgagcttgga
     3061 acaactggac agataatcgt aatagccagc atatctacgg tgtcagtttc tttaataaca
     3121 agtacaggtg ctttgtgtgg cggcaatata actaataatg ggggcgcttt agttactgca
     3181 cgaggcgtgt gctggaatac ttcagcaaac cccacagtgg aaaataataa aaccctggat
     3241 ggaacaggcg gtggggtttt tacaagtgct ctatcaggct taacggccaa tactacttat
     3301 tttgcaaggg cttatgccac aaccagtgca gggacaggat acggcaatca aataagtttt
     3361 acaacaacaa tttctgttgg cgatagttat cagggaggga ttgtgggcta tattttacaa
     3421 agcggtgacc cgggatatat tcccggacaa atacatggaa taatagctgc cacaagcgac
     3481 caaaattcaa ccacgtggtg gggatgtcca atatatggtg aaaccaccgg tacggccata
     3541 gggacaggag ccacaaacac cacaactatt attgcggacc cctgtgcttc accggaaatg
     3601 gctgcgagac tctgtagcga ttttgtgtca ggcggttata acgattggtt tttgcccagc
     3661 aaggatgaat taaacaagct ttatattaac agagccgtga ttggaggatt taatactttc
     3721 aggtactgga gttcatcaaa taatggtatg ggggcgtggg aacaaaattt ttctaatggt
     3781 tctcaggcac aagttgacag acacacccaa aactatgtgc ggcctgttcg ggcattttaa
     3841 aaaaaattgt cattttttta gcaccatctt atagtgcgct attcctgctt cgaaaaaaac
     3901 ttcgccggag acaacaaaac catggcgttg atataaggta caggctttta gttgcgaatg
     3961 caagtaaatg ggcagcccca acgaaataac atcttccaga acttttttca ccagtgttcc
     4021 gcctattcct ttattacggt attccggtaa aacagcaaac cgttctagtt taataccctg
     4081 tgctgttttg cgccagcggg ctgcgccaac gggtgtttca tcatcataaa gcaggtaatg
     4141 tgtggcttcg gcttcgtgtt cgtcgtattc aagttctgct ggcacgccct gttcagcgac
     4201 aaatactgcc tggcgtatgg taaaagcttc tttaaataaa tcctggtcag aaaaagaaaa
     4261 cgacgtgatt ctcaccatgt aagtttttcg tcaaatatac aaaaagataa aatttatagt
     4321 aaaaaactga tgaaaaaatt aatatctttg ttgaaatccg ttgctaaaga aaattattaa
     4381 ccaaaaaata taattatgtt tgaattaccc caattacctt atgcggcaaa tgccctggaa
     4441 ccttttattt ctgaaaaaac catagggttt cactatggca agcaccacca ggcctatgtt
     4501 accaacctga acaagctggt tgcaggtact gagtttgaaa atgccaccct cgaagaaata
     4561 atcaaaaaaa ctaccggggg aattttcaat aatgccgcac aggtatggaa tcatactttt
     4621 tactggaata gtctggcacc taaaccaaaa gcccaacctg agggagcttt agctgaagct
     4681 atcaataaca aattcggttc cttcgacagt tttaaagaaa aattcagcgc tgcctgtgta
     4741 actctatttg gttcgggatg ggcctggctt gtaaaaactc ccgacggaag cctggatatt
     4801 gttcaggaaa gcaatgccgg caaccctttg tgcaatggtc tgatcccaat attgacctgt
     4861 gatgtctggg aacacgccta ttatctggat ttccagaacc gccgccccga ttatgtcgct
     4921 gaattttgga aatacgtgaa ttgggaagaa attgaaaaaa gatttttgta aaacaatgta
     4981 aatcatcgtt tcgctttggt ggccggcaga agaaaaaatt ttttcccgca gtccgccgcg
     5041 gcggaacgca gataaacgca gaaaaggaaa aatagatcag cgtaaataag cgagatcagc
     5101 gggaaataaa aaaggaaaga agttcccgca gataacgcag ataagcgcag aaaaagaaga
     5161 aaataaatca gcgtaaatcc gcgagatccg agggaaataa aaaataggag actcccgcag
     5221 ataaacaaaa aaatatgagt ctggaagaaa ataaaatttc atatattgct agaggatgta
     5281 tctttgaggt ttataatcaa cttggaccag gattgcttga atctgtttat gaatctgcat
     5341 tgtcgtatga attgttgaat cagggtttga aaattgaaac acaagttcca attcctgtca
     5401 attataagga tattccgctt gaaattggat ttagagccga tattattatt gaaaacaaag
     5461 tgattattga aataaaatca atagaaaata ttgctgaagt tcatcacaaa caagtgttaa
     5521 cctatatgaa gcttacaggg attaaactcg gacttttagt taattttaat acagaagata
     5581 ttggtaaatc gatatataga aaggttaata aattataaat aaatcagcgt aaatcagcga
     5641 gatccgcggg aaataaaaaa ggaaagaagt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5761 nnnnnnnnnn natcagcggg aaataaaaaa ggaaagaagt tcccgcagat gacgcagata
     5821 aacgcaaaaa aagaataaac tatgaacaca gaattaaaaa atgccgccat tatcgcatta
     5881 agagattgca tgggactaaa acccgaagaa acccttttgg taattaccga tgaattaaaa
     5941 agagatattg gtgcggcttt gcacgaagcc ggaaaagaac tctgcaaaga gtcgctgctg
     6001 gtggaaataa aatctcgtga gataaacggg caggaaccgc ccaaacagat agccgacctt
     6061 atgaagatgg tggatgctgt tatttgtcct actgcaaaat cgcttacgca caccaacgcc
     6121 cgccgcgagg cctccaaact tggtgtcagg gtggggacaa tgcctggaat cactgtggat
     6181 tgcatggttc gttgcctgaa tgcggatata caaaaaatcc tggacctgac agattatgtg
     6241 gttaaaagaa tgaccggaat aaaacagatc cgggttgtta ccgaaaaagg aacagatatt
     6301 accatgcctg tagaagggcg gaatgtcctg ccaagcaagg gtgttatgct taacaaaggt
     6361 gaaagcggca atctgccttc gggtgaagtt taccttgccc cctgggaagg aaaaacaaac
     6421 ggaaccgttg tggttgacgg ctcaatggcc ggtataggaa tgttgaaaac accaatcacc
     6481 attgaggttg ttgatggcta tgccgaaaaa atcaccggag gggaggaagc tgtgcgattg
     6541 accgagatgc tcgataaagt gggccgcgat gcccgggctg tggcagaatt tggaataggc
     6601 accaattata aagctcaact gacaggcatg atactcgaag acgaaaaagt gttcgggacc
     6661 atacatgtgg cttttggcaa taacatcagc atgggaggaa atatttcggc aagcagccac
     6721 cttgatgggt tagttaaaga ccccgatgtg tattttgacg acattatgat catgaaaaaa
     6781 ggcaaaatgt tagattttga agtatagtaa ctaattagcg taatccacgg gagaaaaaac
     6841 aaagaagatt gccggcagtt aatcagataa ccacataaaa agtaaacaaa ataaaaaata
     6901 cagcaaatgt taaaagccat tattaaaaca gtaaagggcg acatgactgt cagcttttac
     6961 gaaaaagatg cccccggcac agtaaaaaac tttgttgacc tggccaaaaa gggattttat
     7021 aacggattga cttttcatcg tgtaattcct gattttgtga tacagggagg atgtccttac
     7081 ggcaccggtg ttggcggccc cggatatacc ataaagtgtg aaaccaacgg cggcaaccaa
     7141 taccacgacc gcggagtact ttcgatggct catgccgggc gcgacaccgg cggctcacag
     7201 ttttttatct gccacagcag gcaaaacacc gcacacctcg accgcaaaca tacctgtttc
     7261 ggaaaggtta ttgaaggcct ggaagtgata gatgctatct gccagggcga caagattaat
     7321 tctattgaaa tcctggaaga aaaagattga tcagaacttg ttaaggccct ccatcagtct
     7381 tttttctttt tcttcgggac tgaggtcttc gtaggcttgt ttttccttca aatgtttctg
     7441 gagtttttct tcctcaatgc gatggatttc tgcaagatac ttgttgatta cccggttctt
     7501 cagaaagtcg gtagccacgg caggaaacaa ctccagaagc aattcgtctt tgggattgtc
     7561 ggcaagtttt acatcgccat attccggaaa aacaggattg tcttgttttc tgtaattttt
     7621 tgtatcgtaa ggagtttctt ctttaactcc ggcaatttta tatcgaaatt cggggtcgat
     7681 cgggatagga gtgtgcccgt actggccttt taccagattg acaaactgta ttgatttggt
     7741 ggtataaaaa ggctggcctt tgttttcatc aataatgcaa ttaaccgcct gaacacccac
     7801 aatctgactg gtaggtgtaa ccagcggagg acagcctgca gccaaacgta ccgttggaat
     7861 aatttcaagg gtgcggggca acagtttttc aagtttcagg gtctttagct gggcaagcat
     7921 attggtgtac atgcctcccg gaatttcggc aaacttaaca ttttcgttcg gttcgggaaa
     7981 cttgaaatac cgttcaattt tatgacaaca gtccagcaac tcctcttctt tattgctttt
     8041 tgcataagcg atagccagtt caaaaagatc atcaatatcc tgtggcagtt tgtcgcgtgt
     8101 tatatcaaat tcgcggggga acattttgta actatccaca tcggccattt cggtgcgtat
     8161 atcgcggaga atgttgttaa tttttacaac aacttcctga tttacaccgg tttcaacacc
     8221 caatttattg caaaatagct gaatgagttc ataggcaggt gctgccgggc cgccggcaaa
     8281 agtcataatc gaagtgtcga ggatatcaac accattgatg acagccatca gcgaggatgc
     8341 cagcccgtaa ccaggcgtgc agtgtgtatg aaaatcaaca gggatcttaa gtttttcttt
     8401 taacagcttg ataaggcgtc ccgacctgac aggcggaatg agcccggcca tatcttttac
     8461 cgaaatcatg tcggcgccta aggcttccat ttccagggct ttattcaaaa aatattcgtc
     8521 ggtaaaaatg ttattgggaa gcagtttggc acgcagcagc gcttttatac ggtctttgga
     8581 gctgaaatga ggatcaacgg tataacaaac cgtggcatca gcaataccgc cattttcttt
     8641 aacatatttt atgcttgatt taatgttgtc cacatcgttc agcgcatcga aaatccgcat
     8701 gatgccaata ccggattgga tggcataccg gcaaaaccct tcgataactt cttcaggata
     8761 aggattgtag ccaaacaggt ttcttcctct tgaaagagct gtgagtttgc aggaatctcc
     8821 tattgcaact ttgatttttt ccaacctgtc ccagggattt tcgcccagat aacgcataat
     8881 cgaatcagga accgcgccac cccagacttc cattgcgtaa aaattggctt ctttatacag
     8941 ggggataact ttatcaacct gttcctggtt catacgggta gcaaacaatg attgctgacc
     9001 atcacgcaat gtcaaatccc ggattaaaag ttttcttttc ataatatatt gtaaaacatc
     9061 aagttaaata aaaaatagtt ccttaagaat ttcgctgcaa aggtagataa gtttttgaca
     9121 ggcaatggta aaatgagcaa aataatttta tccgataaaa caataaatat tcactttatg
     9181 acggaaaaat aatggaaaaa ataaccatat tgtttttatg actaatttag tatccccaaa
     9241 aagaatatta acactaatcc caaaaaaatg aaaaaaacta ttgttttatt ttgcctgata
     9301 gcttttgcat cattcataat gcaaggatgt ggcggaagca aaaaaaatga caaagtcatg
     9361 accaaagaag attcaattat gcaagccaga gtaaacgaat atgcgacggt taagcttacc
     9421 tgcgacctga gtaaattatc cgttaaggaa aaacaacttg ttgcggcact tattgatatt
     9481 gctgccatta tggatgacct gtactggaaa caaaccctgg gcgacaaaca ggcctttctc
     9541 gacagtataa aaaatgaaaa tgaaaagaaa tttgccatga tcaattacgg gccgtgggac
     9601 aggcttgatg ataacaaacc ctttatggca gcttatggcg aaaaaccttt aggcgctaat
     9661 ttttatccga aggatatcac caaagaagag tttgaaaaat tcgacaacaa agacaaaaca
     9721 ggcttgtaca ctttaatccg ccgcaacgaa gacaaaagcc tgaaagttgt ttggtatcat
     9781 gacgaatata aagaagaatt gcaaaaagca tccgatctgc tgaaaaaagc tgcaggactg
     9841 gccgaggacg acggcttgaa aaagtacctg gagctgcgtg ccgaagcgct gataaccagc
     9901 aattatcaac caagcgacat ggcctggatg gacatgaaaa acaaccgtat cgattttgtc
     9961 gtaggaccaa tcgaaaatta cgaagatgcc ctgtatgaat acaaaaccgc cttcgaatcc
    10021 ttcgtactga taaaagatat ggactggagc aaaaaactgg ataaatatat tggcatgttg
    10081 cccgatattc agaagcaatt acccgtggaa gaaaaatata aaaaagaagt tcccggcacc
    10141 tcgtccgacc tgggtgtgta cgacgccgtt tattatgccg gcgattgcaa tgcgggaagc
    10201 aaaaccatcg ccatcaattt accgaacgac gaacaggtgc aactcgccaa aggatcgcgc
    10261 aggctacagt tgaagaatac catgaaagca aagtttgata atatcatgat gcccattgca
    10321 caaaaattga ccgatccggc acaaattaag aatgtgaaat ttgatgcgtt tttcaataat
    10381 gtcatgttcc acgaagtggc tcacgggctg ggaataaaaa ataccatcaa cggaaaaggc
    10441 actgtgcgcg aagcgctgaa ggacgaatat tcggcccttg aagaagccaa agccgatatt
    10501 ctcggactgt tcatcgttac ccacctgatt ggcaagggag agataaagga aattaccgtt
    10561 gacgactgtt ttgttacctt catggccggc atctttcgtt cggtgcgttt tggggctgcc
    10621 agtgctcacg gtaaggccaa catgatgtgt tttaatttct ttgaaaaagc aggcgctttt
    10681 acccgcaacg aaaaaggatt ttataccgtg gatgtggcaa aaatgaacga agctatgaac
    10741 caatgggcca gcaccatcct ggtattccag ggcaacggcg attatgacgg cgtggcctca
    10801 taccgcaaag aaaacgctac gattaatccg caacttcaga aagaactcga cgggctcaag
    10861 tcggcaaata tccccaaaga tgtggttttt gagcagggga aaaacatact gggcctctga
    10921 tttatttttg ttgcgtcata agaagcggag catttttccg tttatctgaa aatattaata
    10981 cgatgagaaa agtattatgg ctgggcttgt tcctgcttat aacagggatg ccggatgttt
    11041 tttcgcaggc ttatcagctg ccggatgccg ccgctgtcag cagaatcaaa gataatatag
    11101 gcatcctggc cgccgactca atgatgggcc gcgaaagcgg tacgccggga gaatacatgg
    11161 cccgaaaata catagcagcg caattcaaag aaataggcct ggcgccatta tttgatactt
    11221 cctatttcga atcgtttact ttttcggatg tggatttttt agatttggac aacacgatgg
    11281 aggtcaacgg caaaaaactg cagttgtaca gtgattatta ccctattgga ttttcggcca
    11341 acgacaccgt gtccggcgaa atggtttttg caggcacagg catattatgc gaaagtgccg
    11401 ggattaatga ttatataggt ctcgacctga aggggaaaat tgccgttatt gatttggctg
    11461 tgccgggtac cctgctggaa aaaaagaatg tctgggacag ctcgcagaaa gtcgtgcggg
    11521 ttaacagagc catgagcctg ggagcggtcg gggttgtttt tgtttctacc gaaaaaaatt
    11581 atggcacccc atcgaaagat cctaattttt ataaagaaag agtaaaaata cccgtagtgt
    11641 tcgtatgcga tagcagtatt ataatgcctg aaaaccctgg caaagctttc attatcacaa
    11701 gcattgatcg tagtggattg cgcagggcat acaatgttgg gggttttatt gacaacggtt
    11761 ccgaaaatac ggtggttata ggagcccact acgatcatgt gggactgggt tattttggtg
    11821 cgcgcgatcc gggcgacaat accgtgcaca acggagccga cgataacgcc agtggcgtgg
    11881 ccggcgttct ggaactggcc cgcatgttga aatctgcagg accgaaaagt aacaactatg
    11941 cctttttggc tttttcgggc gaagaaaaag gactgattgg ttcagaaaag tttattacca
    12001 atggaagcgt caagaaagaa cgtatcaatt atatggtaga tctcgacatg atcggccgtc
    12061 tggatgccaa gaaaaaaata aaaatctatg gcacagggac cgctgattta tgggaaaatg
    12121 cgctgaatac tatcggcgac aaaggtttta aaattacgcg gatcaaaaca ggtattggcg
    12181 gttcagacca ctattcattc aatcagcacc agatacccgc tatttttatt cataccggcc
    12241 tgcacgaaga ttaccataaa gccgttgatg atactcacct gattaatttt aaaggtgctc
    12301 aagaggttat caaatatgct tatgaaataa tccgttatct cgacgataag gggaaaatta
    12361 cctttacaat tgcgagtatg ctcgatgaga tgatgttgaa aaagtagttt ttatggcatt
    12421 gaaatttcca ccttgtccaa taggcggaag ttcgtgtcga tagcttccat gcgcagcgta
    12481 ttgccgctta ttttcagtgt acagaaatga tgggcgttga caaaaaactg tgattccggc
    12541 aggtctttgg cttttttata gaaaggcgcg ccggctccgg ctgtggtaat ataataaatg
    12601 ccattagaaa ccgctttttc ataacaatgg ttatgtgcgc tgaaaacaat atccactcca
    12661 tacctctcaa aaagcggcac aaggtttttt cggagttttt tgttctgcgt attgtgagga
    12721 cccgagctga acaccggcag atgcataact acca
//
LOCUS       DAOSFP010000011        12516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4227_length_12516_cov_3.886606, whole genome shotgun sequence.
ACCESSION   DAOSFP010000011 DAOSFP010000000
VERSION     DAOSFP010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4227_length_12516_cov_3.886606"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3114)
                     /locus_tag="PLI16_00740"
     CDS             complement(<1..3114)
                     /locus_tag="PLI16_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83115.1"
                     /translation="MNKFYTLFLLLLSGLTLNAQVKVFHENFELSGNGADSVASSGAF
                     YNWSLNSRLFHAGVRCDSNIVTANDTSLLTTNVFSTAGYSNVVLRFSHICKVELFDAG
                     EVEISVNNGPWTKLTGTQYVNPGNSQFVNSGNKFNANTYPLDWSPAVNNLKPTQSWWK
                     NEVFDLSLLAGNSTNVRIRFVLRDGNGNGANLNHGWFIDDIEVLAANSELIPPRITMI
                     TPIITDTIHDTGPFEIAAWIADNSGIDTAYIKYSVNNGSDQYVPMVWIADSTYQGTIP
                     SYTYTNRISYRLYATDNASAHNTTESGNYWFYIKKETPCVEIGTESIQQIYPYSTSFS
                     DARTQILFTAEEIQAASGTSGPINALAFNVASASAITMNGFTIQMQHTSLNTLTGFVN
                     TGWTTVFSGTCMVSDVGWQSVSLQTPFIWDGINNVLINICYDNAAGSGNSPVLSSYAA
                     GKTWTQNAATGAGCSLAAGTTQGYRPNIRFYMSTYILTEDAGVSNFLSPSGIILSGVS
                     HPVEIQIKNYAQDTLKKATVAWELNGVPQPSFIWTGLLAEQVTSAAFTIGNISTGNGS
                     QTIKAWTENPNDSIDEVAANDTLSTYFYACTGFLSGTYTIGSGGDFPTFSSALNSMVN
                     CGISGPVVFNVLPGTYQEQLLIPEIPGASGTNTIVFQSASGDYHDVVLQYAASSANDN
                     YTVFLNGADHITFKNITLAARGTSYANVVRYTGNATYNTFFNNLLSGMITTSTSINLA
                     VVYSPSGAGMCDSVITFDGNIVDNGSYGIYLYGQGSALGQLENRTSVINNLFNNQYMA
                     GLYLYYHNAPEISGNIFISGATYPSGISIYTAYCDNRMTIIKNKITRFYGGYGINTYY
                     CDGVNGKEALIANNMVSILGNNSSYGIYAAYGNYQKIFYNSVNIAAEASTSRPLYLTG
                     TGIYHYIKNNIFAYTGHNSSGLAAYISNTAGVTAMNYNNLYSSGTQLGYWAGIQANLS
                     AWQAASAKDSNSVSVNPYFVSSTDLHTYAQGVDGKATPLSEVTDDIDGHGRHATIPDI
                     GACEFDVP"
     gene            3320..5122
                     /locus_tag="PLI16_00745"
     CDS             3320..5122
                     /locus_tag="PLI16_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOH83116.1"
                     /translation="MPNPDELTEKKEILNRYRNLLRVCKPKTSQKDKLLIRKAFDLAV
                     EAHKDMRRKSGEPYIYHPIDVATIAAEEIGLGTTSVVCALLHDVVEDTDYTLEDIRSL
                     FGDKVAKIIDGLTKISGIFDNKSESLQAENFRKMLLTLSDDVRVILIKLADRLNNMRT
                     LASMPREKQLKIASETSFLYAPLAHRLGLFTIKTELEDLAFKYLEPEIYNTIDFMIRE
                     SEPERKKFITKFVAPIKKDLADKNINAEILTRTKSIFSIWEKMRKKEIPFEEVYDLFA
                     IRIILDVPPEKEKSECFNVYALVTDNYRPNKDRFRDWISIPKANGYEALHTTVMSHTG
                     KWVEVQIRSKRMDEIAEKGFAAHWKYKAGTHGDTGLEDWMAKIRELLQSPESDALDFL
                     DNFKLNLFSDEIYVFTPKGDLITMPSKSTTLDFAYGIHSDIGNRCIGAKVNHRLVPLD
                     YTLKSGDQVEIITSDKQTPKESWYDYATTAKAKSQIKAYVKEEKKKQANDGKIKLKKE
                     FEELKVEFSISNINKLMNFLKYGNPSALFIDAEKDFVGTKEIKACFIEKIPGCVIFHL
                     FPILSPKANTAKAKKKKNPLKKILKGTKLTNNRC"
     gene            5116..5553
                     /locus_tag="PLI16_00750"
     CDS             5116..5553
                     /locus_tag="PLI16_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83117.1"
                     /translation="MLSSKVDNIKYKIARCCNPILGDEVIGLMEKNGEISVHKANCNQ
                     AVEFMTKYGKKIVKSKWLSDDSLQFLAGISIKGFDRLGMIRDITDVISKQLNINIRSL
                     TLETTGGIFEGILTVYIQNTSQLKNLMGNIKTVNGVESVNRID"
     gene            5640..6095
                     /locus_tag="PLI16_00755"
     CDS             5640..6095
                     /locus_tag="PLI16_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOH83118.1"
                     /translation="MKEQVSGNFDDVKQIFIAYLEKNQHRKTPERFTILKEIYAFEGH
                     FDVETLYIKMKNSKYRVSRATLYNTIELLLLCGLVVKHQFGQSSAHYEKSYKFKQHDH
                     IICIDTKKVIEFCDPRIQEIQRSLEQMFNVTITHHALTFYGKCNNKKEK"
     gene            6129..7412
                     /locus_tag="PLI16_00760"
     CDS             6129..7412
                     /locus_tag="PLI16_00760"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOH83119.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDAMTPHYDIIARFQGGPNAGHTIE
                     FNGNKIVLHSVPSGILRDNTVNVIGNGVVLDPVILMNELKIVESFNPAAAQNLRISNR
                     GHLILPVHRALDACFEQAKGKEKIGSTLKGIAPAYADKYNRNGLRAGEILLENFSEKF
                     RTLTEKHQKIFDMYDFNINDFQIDKLPYKEYEKQWLEAIELMKKLQYIDAEMFLNQAL
                     QSGKSILAEGAQGTLLDIDFGSYPFVTSSNTICAGVCSGLGIAPSKIGKVFGVFKAYC
                     TRVGSGPFPTELFDDTGKKLQALGNEFGATTGRARRCGWLDLPALNYAVMINGVTDLV
                     MTKADILSTFDVIKVAQSYNNQTLDYLPFNIDTAPVTPDYTELQGWNVPVDNLQSSKD
                     LPEELLAYIKFIENYVHVPVSFLSLGPDRKQTYQL"
     gene            7425..8987
                     /locus_tag="PLI16_00765"
     CDS             7425..8987
                     /locus_tag="PLI16_00765"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HOH83120.1"
                     /translation="MKKGILFFICLLFALYDVNAQKISDSKVPQDVIISCRYKYPDAT
                     VMYWEKQDENYVARFKMNNQTGNAEFDEKGLWIKTRFEIKEKELPSSVVSYYKENYLA
                     NEYVMSVIEIHKTSQGKSYYYIQLKREGVTQVLPVDLQFDLAGTLIYKNDPGEAKANE
                     GDNKTVNTNTAASEPPDDGSKYLVPDNKVPAVATTHFKSKNKKASGAAWYFRDQKYTV
                     KYNSAGRNALSTYTKEGAWLETRVESSEDKLNQLVTNYLKDNYRQYKIKTVEFVTQPK
                     DKSIEIGMYDKRSNATPPPITKIWFTSAGKYVNVEKPDIVDPNEREAQKRREEKDKKF
                     MEDVDKSGVVYEDAENYNDKVNKKELPSSIHEYIKKNYKEHVINSSRLVSDDELGNVY
                     LIRVKIEGAKYGTQLFFDIAGKFLKKIEESEARMNKEDINMGTSGTEVQESKYGTSDE
                     KISQGELPSEISKYLKKNYPGHEIKESYFKTDKDLGNCFLLIMQKAGEKKITKLFFDL
                     DGNLARTSFENQ"
     gene            9042..10559
                     /locus_tag="PLI16_00770"
     CDS             9042..10559
                     /locus_tag="PLI16_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOH83121.1"
                     /translation="MKRFSTFLIVILCCLKCFMVSGQGVKKIDLVSAENMIYDQSKGD
                     FTLLIGNVIFQHEEILLYCDSAHLYDATNNLEAFGSVHIKASDSTNIYGDSLKYDGNT
                     KIAEMHKNVRLIDNQITLTTEHLTYDLKAKTGKYYDGGKIVDPENTLTSIKGFYYSEK
                     KDFFFNDSVVLVNVEYTILSDSLIYNTASEISRFFGPTTITSKENFIYCEKGWYDTKN
                     DNAEFTKNALLKNEGQQLTGDSIYYNRIKGLGLAYRNVTIVDTTRNSVVKSDFTKYDE
                     KNQFSLATGNALFIQIDEHNDSLFLHADTLIGTFDTATRKAKIMYAFHKVKFYRDDLQ
                     GRCDSLVYNYADSTIRMYYDPVIWTDNNQLSADTVAIQISNSLIDKMFLRKASYVVSC
                     DDTTESRYNQVKGINMTGYFVKGELKKIHVSGNAETIYFMRDGSERKIGINKAIATNL
                     DIYITDEKITSITFLEDPVATLYPDKELSKQELFLKNFRWLENFRPKTKTDIFKR"
     gene            10567..11262
                     /locus_tag="PLI16_00775"
     CDS             10567..11262
                     /locus_tag="PLI16_00775"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOH83122.1"
                     /translation="MHLFYTPHIDPQFHVLSQEESKHCRVLRIKTGDFIYLTNGKGTL
                     CKAIVTDNATGIYHTKIVEQHEEFNKRPYHLHIAIAPTKNTDRFEWFLEKVTELGIDE
                     ITPLICEHSEKSHLKYERLQKVLIAAVKQSFTAYLPRLNNQSKFTDFVKTAFSGQKFI
                     AYCGESPQHLRVACVPEQEVTILIGPEGDFSEEEITLAKNSGFIPITLGKNRLRTETA
                     GVFACSVVNTVNI"
     gene            11267..11926
                     /locus_tag="PLI16_00780"
     CDS             11267..11926
                     /locus_tag="PLI16_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOH83123.1"
                     /translation="MKKLFRLLLCSLIVFSVPRLYSQNLQLALAKYKGGGDWYANPTS
                     LANLVKFCNQNLGTNLNPDYATVDVGSPELFNYPMLHLTGHGNVVFSNQEASNLRNYL
                     ISGGFLHISDNYGIDLFIRREMKKVFPELVFVELPFNHPIYHQKYDFPNGPPKIHEHD
                     NKPAQGFGLIYEGRLLCFYDYECDLGDGWEDQAVHKDSEPTRLKALQMGANIIQYVFM
                     K"
BASE COUNT         3880 a         2608 c         2651 g         3377 t
ORIGIN      
        1 cgggacatca aattcgcatg ctccaatgtc ggggattgtg gcatggcgtc catgtccatc
       61 tatatcatcg gtaacttcac ttagcggcgt agcttttccg tcaaccccct gggcgtatgt
      121 atgcagatcg gtggatgaaa caaaatacgg gttgacggac accgagtttg aatccttggc
      181 gctggcagcc tgccatgcgc tgagattggc ctgtatgccg gcccagtagc ccagttgagt
      241 gccggaggaa tacaaattat tatagttcat agccgtaaca ccggcggtgt tggatatata
      301 tgctgccagg ccactgctgt tatggcctgt ataagcgaaa atgttatttt taatataatg
      361 ataaattcct gtgcctgtca gataaagtgg gcgcgaagtg ctggcttcgg cagcaatatt
      421 gacgctgtta taaaatattt tctgatagtt accataggca gcatatatgc cataagaact
      481 gttgttgcca agtatgctca ccatgttgtt ggctattaat gcctcttttc cgttaacacc
      541 gtcgcagtaa taggtattaa tgccataacc gccataaaac cgggtaattt tattttttat
      601 tatggtcatg cggttgtcgc agtacgctgt gtaaatgctg atgccggaag ggtaggtggc
      661 acctgatatg aatatattgc cggaaatttc aggagcatta tggtaataca aatataatcc
      721 cgccatatat tgattgttaa acaagttgtt tattacggag gtcctgttct ccaactgtcc
      781 taaagcgctt ccctggccgt aaaggtatat tccgtaggaa ccattatcaa cgatgttgcc
      841 atcgaatgtt attaccgaat cgcacatgcc tgccccactt ggggaatata ccaccgccag
      901 attgatggag gtgctggtag tgatcattcc ggagagtaga ttgttgaaga acgtattata
      961 ggtggcatta ccggtatatc tgacaacatt ggcataagaa gtgccacggg cggccagggt
     1021 aatattttta aaggttatat gatcagcgcc attcaaaaac acagtatagt tgtcattggc
     1081 cgagctggca gcatattgaa gcactacatc atggtagtca ccgcttgctg attgaaaaac
     1141 aatggtgttg gtcccgctgg ccccgggtat ttctggaatg aggagctgtt cctggtaggt
     1201 cccgggcaaa acattgaaaa ccaccggacc ggaaattccg cagtttacca tcgaatttaa
     1261 tgctgaagag aaggttggaa aatctcctcc tgaacctatt gtatatgtcc cgctcagaaa
     1321 accggtgcag gcataaaaat atgtactcag cgtatcgttg gcagctactt cgtcaatgct
     1381 gtcgttgggg ttttctgtcc aggcttttat ggtttgactt ccgttgccgg tactgatatt
     1441 gccgatggta aatgcagcag aggtaacctg ctcggcaagt aagcccgtcc aaatgaatga
     1501 gggttgtgga accccattga gttcccaggc cacggtggct tttttcagag tgtcctgtgc
     1561 atagtttttt atttgtatct caacagggtg gctcacaccc gacaaaataa tacccgatgg
     1621 ggacaaaaaa ttgctgacac cggcatcctc cgttaagata taagttgaca tataaaacct
     1681 gatattcggt cggtatcctt gcgtggtacc ggcggccagc gaacagcctg caccggtagc
     1741 tgcattttgt gtccaggttt tccccgcggc atagctgctc agcaccggtg agttgcccga
     1801 gccggcagca ttgtcataac agatatttat taatacattg ttaataccat cccagataaa
     1861 aggagtctgt aacgagacag actgccaacc tacatcggat accatacagg tgccagaaaa
     1921 aactgtggtc caacccgtat tcacaaagcc ggttaacgta ttcagactgg tgtgttgcat
     1981 ttgtatggta aacccattca tagtaatggc tgaagcacta gccacattaa aagcgagagc
     2041 gttgattggt cccgaggttc cgctggctgc ctgtatttct tcggctgtaa ataaaatttg
     2101 tgtccgggca tcgctgaaac tggtggagta ggggtagatc tgttgtatcg attccgttcc
     2161 tatttccacg cagggagtct cttttttgat ataaaaccaa tagtttccac tttcggtagt
     2221 gttgtgtgca gatgcattat cggtggcata caggcgataa ctgatccggt tggtataagt
     2281 ataactcggg atagttccct gataggtgct gtcggctatc cataccatgg gaacgtattg
     2341 gtcggatccg ttgtttacgg aatattttat ataggcggtg tctatccctg aattgtcggc
     2401 aatccaggct gctatctcaa aggggcctgt gtcgtggata gtatctgtaa ttatcggtgt
     2461 tatcatcgtg atgcgcggcg gtatcagttc tgaattggca gccagcactt cgatatcgtc
     2521 gataaaccag ccgtggttca ggttggcccc gttgccgttt ccatcgcgga gcacaaacct
     2581 tattcttacg ttggtactgt tgccggccag cagcgaaagg tcaaaaacct catttttcca
     2641 ccacgattgg gtgggcttca gattgttaac cgccggactc caatcaaggg gataggtgtt
     2701 ggcattaaac ttgttcccgc tgtttacaaa ttgcgaatta cccggattta catactgtgt
     2761 ccctgttagt tttgtccaag ggccgttatt tactgatatt tccacctccc ctgcatcaaa
     2821 cagttccact ttgcatatat gtgaaaaccg caaaactacg ttggagtaac cggcagtgga
     2881 aaacacattg gttgtcagca acgacgtatc attggctgta acaatatttg aatcgcatcg
     2941 gacgccggca tgaaacaacc gggaattcaa cgaccagtta tagaatgccc cggaacttgc
     3001 cacgctatcg gcgccatttc ctgataattc aaaattttca tgaaagactt ttacctgggc
     3061 atttagcgtt aacccggata agagtaataa aaacagcgta taaaatttgt tcataatgca
     3121 agttttaatt atcaggggaa ttttcttaaa aacatcattc taaaaacaaa aataagaaaa
     3181 ttgcaaggca aaaagccttt cttttgacat aaataataat aaacaatgaa ttattaattc
     3241 cggcaaagtt ttgcagaatt attcaatata ctttccttga tgcttatttt tgaattaatt
     3301 ttgtataaaa attgttagta tgccgaatcc tgatgaattg accgaaaaaa aggagatcct
     3361 taaccgttac cgaaatctgc tgcgggtatg taaacccaag acttcacaaa aagataaact
     3421 gctgataaga aaagctttcg acctggcggt ggaagcccac aaagatatgc gtcgcaaatc
     3481 cggtgagccg tacatttatc acccgattga cgtagcaact attgccgctg aagaaatagg
     3541 cctgggaacc acttccgtcg tgtgcgccct gttacatgat gtggtggagg atactgacta
     3601 tacgctggag gatatccgca gtttatttgg cgataaagtg gccaaaatta ttgacgggtt
     3661 gaccaagatt tcagggattt ttgataataa gagcgaatcg ctgcaggcgg agaattttcg
     3721 caaaatgctg ctcaccttgt ccgacgatgt cagggtaatt cttataaaac tcgccgatcg
     3781 tttgaataat atgcggacac tggcatcaat gccgcgggaa aaacaactga agattgcctc
     3841 ggaaacttcg tttttatatg ctcctttggc gcacaggtta gggcttttta ccataaaaac
     3901 cgagctggaa gatctggcat tcaaatattt ggagccagaa atatacaata cgattgactt
     3961 catgatcagg gagtcggagc cggaacgtaa aaagtttatt accaagtttg tagctcccat
     4021 taaaaaagac ctcgcagaca aaaatatcaa cgccgaaatt cttacgcgca ccaagtcgat
     4081 tttctctatc tgggaaaaaa tgcgcaaaaa agaaatccct ttcgaagagg tgtatgatct
     4141 ttttgccatc aggataatcc tcgacgttcc accggaaaaa gaaaaatcag aatgttttaa
     4201 cgtgtatgcc ctggttaccg ataactatcg tcccaataaa gaccggttcc gtgactggat
     4261 ttccataccc aaagctaacg ggtacgaagc gctgcatact actgtcatga gccatacggg
     4321 caaatgggta gaggtgcaga tacggtcgaa acgcatggac gaaatagccg aaaaaggctt
     4381 tgccgcacac tggaaataca aagccggaac tcacggcgat acgggccttg aagactggat
     4441 ggcaaaaatt cgcgaacttt tgcaaagccc cgaatccgat gcgttggatt ttctggataa
     4501 tttcaaactg aacctctttt cagacgaaat ctatgtattc actcccaagg gcgatctgat
     4561 taccatgcct tcaaaatcaa ccacccttga ttttgcttat ggcatacatt ccgacatcgg
     4621 aaaccgctgc atcggggcaa aagtaaacca cagacttgta cccctcgact ataccctgaa
     4681 aagcggcgac caggtggaaa ttatcacttc cgacaagcaa acacccaaag aaagctggta
     4741 tgactatgct accaccgcta aagccaaatc gcagataaaa gcttatgtaa aagaagaaaa
     4801 gaaaaaacag gccaacgacg gaaaaataaa attaaaaaaa gaatttgaag aactgaaagt
     4861 tgagttcagt attagcaata tcaacaaatt gatgaatttt ctcaaatatg gtaatccttc
     4921 tgcattattt atcgatgcag aaaaggattt tgttggtaca aaagagataa aagcctgctt
     4981 tattgaaaag atacctggct gcgttatatt ccatctattc ccaatccttt cgccaaaagc
     5041 aaacacggcg aaagcaaaaa agaaaaagaa tccgttgaaa aaaattctga aagggacaaa
     5101 gttaacgaac aaccgttgct aagttcaaag gttgataata taaaatacaa gattgcccga
     5161 tgctgcaacc ccattttagg cgatgaggtt atcggactta tggaaaaaaa tggggaaata
     5221 tccgtacata aggccaattg taatcaagcc gtggagttta tgaccaaata cggcaaaaaa
     5281 attgtgaaat caaaatggtt atcggacgat tcgctgcaat ttctggcagg tatatccatc
     5341 aagggtttcg acaggcttgg catgatacgc gatatcactg atgtaatatc caaacaactg
     5401 aacatcaata tccgttcgtt aaccctcgaa accaccggcg gcatctttga aggaatactc
     5461 acagtatata ttcagaacac cagccagtta aaaaatctta tgggaaacat caaaacggtt
     5521 aatggcgttg agagtgtaaa caggatcgat tagtttctgc ttttgattgg aataattatt
     5581 gattcattat ttagaatcaa taaaatttgt atttttatgc aaaaatattg tttatggttg
     5641 tgaaagaaca agtttcagga aatttcgacg atgtcaaaca gatttttatt gcttatctgg
     5701 aaaaaaatca gcatcgtaaa accccagaac ggtttaccat attaaaagaa atatatgctt
     5761 ttgaagggca ttttgatgtg gaaacccttt atatcaaaat gaaaaacagc aaataccgcg
     5821 ttagtcgcgc caccttgtac aataccattg aactgttgtt attgtgcggt ctggtggtaa
     5881 aacatcagtt tgggcaaagt tctgcgcatt acgaaaaatc atacaaattc aaacaacacg
     5941 atcatattat ttgcatcgac acaaaaaaag taatagaatt ttgtgatccc cgcattcagg
     6001 agatccaaag gtcactggaa caaatgttta acgttactat cacgcatcat gcactcacgt
     6061 tttatggaaa gtgcaacaac aaaaaagaaa aataatttat actgaacacc taatctatcc
     6121 aatttgcaat gaaagttgat gtaattctgg gccttcaatg gggcgacgaa ggtaaaggaa
     6181 aaattgtgga cgccatgaca cctcattatg atattattgc ccgttttcag ggtggcccca
     6241 atgccgggca taccattgaa ttcaatggca ataaaattgt cttgcactcc gttccctcgg
     6301 gcatattgcg cgataatacc gtcaatgtta tcggcaacgg ggtggtgctc gatcctgtaa
     6361 ttcttatgaa tgaactgaaa atcgtagagt catttaatcc tgctgccgcc caaaatctcc
     6421 gcatttcaaa caggggccac ctcatcctcc ccgttcacag ggcgctggat gcctgttttg
     6481 aacaagctaa agggaaggag aaaattggct ccacactcaa aggtatagct cccgcctatg
     6541 ccgataaata caaccgcaac gggttacggg ccggtgaaat tttgctggaa aatttttcag
     6601 agaaattcag aactcttacc gaaaaacatc agaaaatttt tgatatgtac gattttaata
     6661 tcaacgattt tcagatcgat aagctgcctt acaaagagta tgaaaagcaa tggctcgaag
     6721 ccattgagct gatgaaaaag ctgcaataca ttgatgccga aatgtttctt aatcaggccc
     6781 tgcaatcagg caaaagcata cttgccgaag gcgcccaggg cacgttgctc gatattgatt
     6841 ttggttctta tccttttgtt acatcttcaa ataccatttg tgccggcgtg tgttcgggac
     6901 tgggaatagc cccatcgaaa atcggaaaag ttttcggtgt ctttaaggct tattgtacca
     6961 gggtgggaag tggccctttt ccaaccgaac tttttgatga taccggcaaa aaacttcagg
     7021 cgcttggcaa tgaatttggc gctaccaccg gacgggcgcg ccgctgcgga tggttagatt
     7081 tgccggctct taactatgcc gttatgatca acggtgttac cgacctggtt atgaccaaag
     7141 ccgatatctt aagcactttt gatgtgataa aagtggccca atcctataat aaccaaaccc
     7201 tggattatct gccctttaac attgatacag cccctgtaac acccgattat actgagctgc
     7261 agggctggaa tgttcctgtg gataatctcc aaagcagtaa agatctgcca gaagaattat
     7321 tggcttatat aaaatttatc gaaaactacg ttcatgttcc ggtatctttt ttatctttag
     7381 gacctgatag aaaacaaact tatcaattat aaaaaacagc accaatgaaa aaaggaatct
     7441 tattttttat ctgtttacta tttgcattgt acgatgtaaa tgcgcagaaa atctctgatt
     7501 caaaagtccc tcaggatgtg attatcagtt gcaggtataa ataccctgat gctaccgtga
     7561 tgtactggga aaaacaagac gaaaactacg tggcacgttt taaaatgaat aatcaaaccg
     7621 gcaatgccga atttgacgaa aaagggctgt ggataaagac cagatttgaa ataaaagaaa
     7681 aagaactgcc cagctcggtt gtaagctatt acaaagaaaa ctatctggca aatgaatatg
     7741 taatgtcggt aatcgaaatt cacaaaacca gtcagggcaa aagttattat tatatccaat
     7801 tgaaaagaga aggtgttacc caggtgctgc cggttgacct gcagtttgac ctggccggca
     7861 cccttatcta taaaaatgat ccgggtgaag caaaagcaaa tgaaggcgat aataaaacgg
     7921 tcaacaccaa taccgcagcc agcgaacccc ctgatgatgg aagcaaatat ttagtgcccg
     7981 ataataaagt gccggccgtt gccactactc attttaaaag caaaaacaaa aaagcttccg
     8041 gcgctgcctg gtattttcgg gatcaaaaat ataccgttaa atataattct gccggacgaa
     8101 atgccctttc tacctatacc aaagaaggtg cttggctcga aacacgcgtt gaaagcagtg
     8161 aagacaaact gaaccagtta gtgactaatt atttaaaaga caactaccgt cagtataaaa
     8221 tcaaaacggt ggaattcgtt actcaaccta aagacaaatc tatcgaaatt ggtatgtatg
     8281 ataaacggag caatgcaacg ccaccaccca tcacaaaaat ttggttcact tcggcaggca
     8341 agtatgttaa cgtggaaaaa cccgatattg ttgaccccaa cgaacgcgag gcacaaaaac
     8401 gccgcgaaga aaaagataaa aaattcatgg aagatgtgga caaaagcggc gtagtatacg
     8461 aagatgccga aaattataat gataaagtaa ataaaaaaga actgccgtca tctatccatg
     8521 aatatatcaa gaaaaattat aaagaacacg taatcaacag ttctcgcctg gttagcgacg
     8581 atgaactggg caatgtttat ctcatcaggg tgaaaataga aggagctaaa tacggcaccc
     8641 agttgttttt tgatattgcc ggaaaattcc tcaaaaaaat tgaagaatcg gaagcccgta
     8701 tgaacaagga agatattaat atgggtactt cgggaactga ggtgcaggaa tcaaaatacg
     8761 gcacctcgga tgaaaaaatc agccagggag aattaccgtc cgaaatttcc aaatacctga
     8821 aaaagaacta tcccggccac gaaatcaaag aatcctattt caaaaccgac aaagacctgg
     8881 gaaattgctt tttactaatt atgcaaaaag ccggtgaaaa aaagattact aaattatttt
     8941 tcgatctcga cggtaatctc gcaagaactt cattcgaaaa ccagtaagcc ccggcaacgc
     9001 atcctgtgca ttatgctcat ggctttttac caattgcaaa aatgaaacgc ttcagcacat
     9061 ttttgattgt catcctgtgt tgcctaaagt gttttatggt cagcggccag ggcgtgaaaa
     9121 aaattgattt ggtcagcgcc gaaaacatga tttatgacca gtcaaaaggt gattttaccc
     9181 tgctgattgg caatgtcatt tttcaacacg aagaaattct gctctactgc gacagcgcac
     9241 acctgtatga tgctaccaat aacctcgagg cattcggttc cgtgcatatc aaggcgagcg
     9301 attctaccaa tatttacggc gattccttaa aatatgacgg aaatacaaaa attgccgaaa
     9361 tgcacaaaaa tgtccggctt atcgataacc agatcacgct gacaaccgaa cacctgacct
     9421 atgaccttaa agcaaaaaca ggaaaatact acgatggcgg gaaaattgtg gaccccgaaa
     9481 atactctcac cagcatcaag ggcttttatt attcggaaaa aaaagacttt ttcttcaacg
     9541 attcagtggt gttggtaaat gttgaatata ctatactgtc cgactcattg atatacaaca
     9601 cagcctctga gatttcgcgt tttttcgggc ctaccaccat taccagcaaa gaaaatttta
     9661 tctattgcga aaaaggttgg tacgacacca aaaacgataa cgcagagttt acaaaaaatg
     9721 ccttgcttaa gaacgagggc cagcaactta caggcgacag catttattac aaccgcatca
     9781 agggacttgg cctggcatac agaaatgtga ctattgtgga tactacccga aactcagtgg
     9841 ttaaaagcga ttttacaaaa tatgacgaaa aaaaccagtt cagccttgct accggcaatg
     9901 ccctgtttat tcagattgac gaacataatg attccctgtt tctgcacgcc gatacattga
     9961 taggaacctt tgacacagct acccgcaaag caaaaataat gtatgccttc cacaaggtga
    10021 agttctatag agacgacctg cagggccgtt gcgactctct ggtgtataac tatgccgact
    10081 ccaccatacg gatgtattac gatccggtta tctggaccga taacaaccag ctgtctgccg
    10141 acaccgtggc aatccagatt agcaacagcc ttatcgataa aatgtttttg cgcaaggcca
    10201 gttatgtggt ttcgtgcgat gatactaccg aaagccgcta taatcaagtc aaaggaatta
    10261 atatgaccgg gtatttcgtt aagggagaac tgaaaaaaat tcatgtgtcg ggcaatgccg
    10321 aaaccatata cttcatgcgc gatggcagcg aaagaaaaat tggtatcaac aaggcaattg
    10381 ccaccaatct tgacatctat attaccgatg aaaaaataac ttccattaca tttctcgaag
    10441 atcccgtggc aactttatat cccgataaag aattgtcgaa acaggaactt tttctgaaga
    10501 atttcagatg gcttgaaaat tttcgcccaa aaacaaaaac cgacattttc aaacgctgag
    10561 ttttttatgc accttttcta cacccctcat atcgacccgc aatttcatgt gctgagccag
    10621 gaagaatcaa aacattgccg tgtgctgcga atcaaaaccg gagatttcat ctaccttacc
    10681 aatggcaaag gcaccttgtg caaagccata gttactgata acgcaacagg aatttatcat
    10741 accaaaatag tagaacagca cgaagaattt aataaaaggc cataccatct tcatattgct
    10801 attgcgccaa caaaaaacac cgaccgcttt gagtggtttc tggaaaaagt tacggaactg
    10861 ggcattgatg aaattactcc cctgatatgt gaacattccg aaaaatcgca tctgaagtac
    10921 gaacgcctgc aaaaagtact gattgctgca gtaaaacagt ctttcacggc ctacctgcct
    10981 cgtttgaaca atcaatcaaa atttaccgat tttgtgaaaa ctgctttctc cggccagaaa
    11041 tttattgcct attgtggtga gagcccgcag catttgagag ttgcttgtgt tccggaacaa
    11101 gaggttacca tccttattgg gcccgaaggc gatttttctg aagaggagat tacactggca
    11161 aaaaattcag ggtttattcc aataacattg ggcaaaaacc gtttaaggac tgaaaccgcg
    11221 ggtgtttttg cctgtagcgt tgtaaataca gttaatatct gaggttatga aaaagttgtt
    11281 ccggttgtta ctttgttccc tgattgtttt ttcagtccca cgcctgtatt cacagaatct
    11341 tcagttggca ttggctaaat acaagggtgg tggcgactgg tatgcaaacc ccacctcttt
    11401 ggccaacctg gtaaaatttt gcaatcaaaa cctaggtacc aacctgaacc cggattatgc
    11461 taccgttgat gtgggcagcc ccgaactgtt taactatccc atgctgcacc tcaccggtca
    11521 tggcaatgtg gttttttcaa accaggaggc ttctaatctt cgcaattacc ttatttcagg
    11581 agggtttctg catatctccg ataattatgg tattgatttg tttatccggc gcgagatgaa
    11641 aaaagttttt cctgagcttg tttttgtgga actccccttt aaccatccta tttatcatca
    11701 aaaatatgat tttccgaacg gccctcccaa gattcacgaa cacgacaata agcctgccca
    11761 gggtttcggt ctgatctatg aaggccggtt gttgtgcttt tacgattatg aatgtgattt
    11821 gggcgatggc tgggaagacc aggctgttca caaagattcg gagcccacac ggctgaaagc
    11881 cttgcaaatg ggggcaaaca taattcaata tgttttcatg aaataggttt tcttgacaaa
    11941 agcaattttc caacaattga ttccatgttt tctgtttgtt tcgattgttt tatttattga
    12001 aagtttgagt ttgcaacacc catacaattt taatagtccc aaaaagcctg gagattaata
    12061 atttgtgaat gtgtggacgt tgcggccaac aggtccggta aacctgcaaa gaaaatttat
    12121 taaacgacaa attaccggat taccgttttt tttgcgaagc tgaccgccgg ttgcctggcc
    12181 accgcgccgc tatttacaac cagttgcatt atatgcaaac atttaatcgt gctgtgatta
    12241 agaaaaacct ttattaataa gggttacatc atgcgaaact taattttcta ataaataggt
    12301 ttacacgcgt tcctcactac accgttgtta cagtattaac ggtaaatgaa gtgtaatgaa
    12361 atggggaata tccttgtttt taattatttt aatgggctga atagttacaa aaaaaagagg
    12421 cggataaatg tccgcctctt ttttgttgaa aaattaagaa ttattcaaca atgagttttt
    12481 tggtaaatct gccaccatct gaaagcaggg taacaa
//
LOCUS       DAOSFP010000012        12472 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4247_length_12472_cov_4.144238, whole genome shotgun sequence.
ACCESSION   DAOSFP010000012 DAOSFP010000000
VERSION     DAOSFP010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4247_length_12472_cov_4.144238"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="PLI16_00785"
     CDS             complement(<1..1054)
                     /locus_tag="PLI16_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOH83124.1"
                     /translation="MKTILNLLLLCVTILFPVCLSGQKQIVSIFPDPVEITYSKGRFI
                     LSPQTKIYVDDMETSGKDAALFNEYLTENYGFSLSINQSQKYDSNSIVIKKTANKELP
                     QDAYKMNISNAFIVIEGRDAGVFYALQTIKHLLPPYKTEIIKIRNLSIYDYPAYSWRG
                     MHLDVCRHFFTVEEVKKYIDLLAFYKMNVFHWHLTDDQGWRIQIDKYPKLTSIGGYRN
                     RTLIGHAYDTVQRYNSIRYGGFYTKEQIREVIAYATRRHVQIVPEIEMPGHALGALSA
                     YPEFSCTGGPFEAAGSWGVFDDVFCTKDKTIRFLKDVLAEVMELFPGKYIHIGGDECP
                     KTRWEKCPECHKVMKRE"
     gene            complement(1110..2039)
                     /locus_tag="PLI16_00790"
     CDS             complement(1110..2039)
                     /locus_tag="PLI16_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOH83125.1"
                     /translation="MKKIAAGIDIGGTNTVTGFVDSDGNCLYKTTISTPGFDDPAVFV
                     TEVSRIVKGFFLSNTDATLIGIGIGAPNGNYYNGSIEFAPNLKWKGVVPLVGLFRQHF
                     DVTVILTNDANAAAIGEMVYGNAKGMKNFIEITLGTGVGSGIVVDGNMVYGHDGFAGE
                     IGHTIVFPGGRVCGCGRQGCLEAYCSAGGIVKTYAELLAKEGKLPDGKMTPYTISKLA
                     EKGDQTAIDTYEYTGEILGLGLSNSVAHTSPEAIFIFGGIANAGEILFRPLKESFEKN
                     LLPIYKNKVKILSSGIKENDAAILGAASLVWKQ"
     gene            complement(2036..3439)
                     /locus_tag="PLI16_00795"
     CDS             complement(2036..3439)
                     /locus_tag="PLI16_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOH83126.1"
                     /translation="METEQRTKYTYPMIALTTLFFMWGFITVLNDILIPYLKGVFELT
                     HFQANLVQFAFFGAYFIGSLIYFLISLSFGDPINKIGYKNGILTGLAISAIACFLFYP
                     AANTFHSFGFFLFALFLLGIGFTVLQIAANPYVAVLGSPETASSRLNLSQAFNSLGTT
                     LAPVLGGFFIFEYFASKANVGAESVKVPYLILGSVLILLMIFIWFSKLPALNSHEHIE
                     KKAGALKHPNLVFGMFAIFFYVGAEVTIGSNLVSFLKLPEIVGFSEVEASNYLAYYWG
                     GAMIGRFLGAISLSNMKKTGKYLLMLLMAVLTFLVIFLITWYMKGLTFDRVWYFLIFI
                     VANYCLFMLGRSLPARTLAYFSVVAFCLLLAAVNTNGSIAMWCVLGIGLFNSIMWSNI
                     FTLAIDGLGRYTSQGSSLLVMMILGGALLPPLQGLTADNLGVHASFVVPMFSYLYLLF
                     YGLKGYQKKKSLKTESL"
     gene            complement(3489..4799)
                     /locus_tag="PLI16_00800"
     CDS             complement(3489..4799)
                     /locus_tag="PLI16_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOH83127.1"
                     /translation="MNEILESINLPLLQDADLKNKVVLVRVDHNVVKKGIIHDPYRID
                     ATISTLYYINAKGGKIILMTHAGRPKNKKTGEIAVSDDTSVQPIVDYLQNKLHITIKV
                     PEFQQYGTHGYLDVETSINHLIRDLNDHTIDMIYLPNTRWFFGEEAKGAEADRFAYQL
                     AGLADIFVNDAFGSWQAHASTVNVAKYLPAYAGFLLQKEISNLQRIYKPDQPFVSVVA
                     GSKFDTKVDSLNALLKVSDHLVLGGVIYNAFLCAKYGFTIEGIEPEDMELAKKFVEYA
                     AQFPGKLIELPYIVESDTMEGLRPGQYRVHNIKDIKAGTKLNYVLDVDPKSFQDPEVK
                     KVFLEAKTIFVNAVMGFTPHFNEGTIALDELIDKNNNAVKLYGGGDTLQELKRLLPGL
                     YIVALDSPKYYIFTGGGAVLKAIQEGNVLGLAPVKALMKSKESI"
     gene            complement(4792..5334)
                     /locus_tag="PLI16_00805"
     CDS             complement(4792..5334)
                     /locus_tag="PLI16_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH83128.1"
                     /translation="MNFLELTKLRQSSRKYEDRLVEREKIARCIEAARLAPSACNSQP
                     WTFVVADQPELKQKIAGETFSSVSPFNRFALQAPVMVAIVTEKPSLLSRFGSRIRNKD
                     FYLFDIGMAAEHFCLQAAEEGLGTCILGWFNEKNVMKLLNIPKNKRVGLLITLGYSTS
                     RIREKTRKPLNKILKYNSYE"
     gene            complement(5331..5858)
                     /locus_tag="PLI16_00810"
     CDS             complement(5331..5858)
                     /locus_tag="PLI16_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOH83129.1"
                     /translation="MEQIEKKIHYTPEDLQMAYSTHLKKMYPVRSRMLLVIAAVSFII
                     GCILLFYEHKNGSVDYTNWAAWFLIVYGVAIAALYFYNQHNLGKRMFSKMPDFAKPFS
                     YVFSPEQIQVSSDNINNTNKWEFYQSALITSEIIMVYPNKFRFNLFPKKYFSDEEFEL
                     LKQWVRAKIKTKEVK"
     gene            complement(5944..6951)
                     /locus_tag="PLI16_00815"
     CDS             complement(5944..6951)
                     /locus_tag="PLI16_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH83130.1"
                     /translation="MKKLKPVILFALCCLFLSSCVSLKKFQEAENNQKNCMEENKTLK
                     GKVMEYVTRLTELTATADKLKKQNELLVADSINKGMKLRDLTHKYQELSMAYDTLQNN
                     NLKLLKGNKFETQKLISELEKTKETLQKKEDDLKKLESELNTKLANLNEMQLKLDEKQ
                     QKLNELQTILDKKDSAVKALKNKVSEALLGYENNGLTVKQMNGKVYVSMDEALLFASG
                     RYEVASKGADALKKLAKVLESNVDINIMVEGHTDNVPYKGTDQLLDNWDLSVKRATTV
                     VRILLANSGIAPVRITAAGRSEYFPVDKASTSEARSKNRRTEIILTPKLDELLKIIES
                     N"
     gene            complement(7182..7949)
                     /locus_tag="PLI16_00820"
     CDS             complement(7182..7949)
                     /locus_tag="PLI16_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOH83131.1"
                     /translation="MKKIVTYNVNGIRAATSKGLLDWIASVNADIVCFQETKAQPEQI
                     PTLEFNALGYHNYWFSAKKKGYSGVGIITRQEPDHVVYGMGIPKYDDEGRVLRADYGD
                     ISVLSVYHPSGSSGDDRQAFKMVWLDDFLDWVNELKKSRPNLIICGDYNICHKPIDIH
                     DPIRNATSSGFLPEEREWLSKFIDSGFVDGFRAFNNEPKQYTWWSFRANARAKNLGWR
                     IDYHMVSEPIRHLMKRAVILPEAKHSDHCPSILEVDF"
     gene            8155..8751
                     /locus_tag="PLI16_00825"
     CDS             8155..8751
                     /locus_tag="PLI16_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH83132.1"
                     /translation="MALFLKKKSIELPDPLTDQELIELYRQTGDTAHIGELFDRYAHL
                     VYGIALKYFNQEDDRREAVMQIFEKLYVALKDHEIENFKCWLYSVAKNHCLMILRKEN
                     SVSFNEQLQLKNFQQDFMDFDGNFSLNHAEDEIIDKKLPEAIKKLSDEQRICIELFYL
                     HEKSYKEVAETTQYSMNQVKSFIQNGKRNLKLMLENIK"
     gene            8748..10004
                     /locus_tag="PLI16_00830"
     CDS             8748..10004
                     /locus_tag="PLI16_00830"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83133.1"
                     /translation="MKRSDKRNRIFGPSGCISEEGISLYLKNQLPAKEKNLLEQHLAG
                     CLLCNEAMEAYEKFYEHGKTADDLFAIKSSLKSTYSNKTANKRNSQRTLITTLSIAAS
                     IILIFTGYYFINLQTKNENKEISYTAPKNLEKPIVGSEQKNPQELINQPAEKTSVKED
                     KTKSIPSQAPQNADYLYKTTTGTDRETSVAATGTAKVVADEAPGYFADTPDKKEEYRN
                     DIYAGDYDQGLVLGGVATAESKADTKNAKAVNQEIAVSSAPLEAEKAVSEKDLALESY
                     ENAVDKVVLSGKKTARKEVSRSQIKTPANTALSYYYSGQYKKAIPEFNKKVARNSQDY
                     ESIYYLAMSYYYEGQKDAALPLLDTILKKNSNPYYDLALWQKAIILTEKKQTAEARTL
                     LNELVDRGGNLKNDALQKLDELDKAE"
     gene            9982..11292
                     /locus_tag="PLI16_00835"
     CDS             9982..11292
                     /locus_tag="PLI16_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOH83134.1"
                     /translation="MNLIRPNKDFPKPVLNKVFALVDCNNFYASCERLFNPKLEKKPL
                     IVLSNNDGCVIARSNEAKSIGIKMGAPAFEFKELIARHGVHVFSSNYALYGDISNRIM
                     AILFEMAPEVEVYSVDEAFLNLSGMDPEAINLLAANIRKTIKKWVGIPVSVGIGPTKT
                     LAKIANHLAKKNPAYANVANLCSIENTDDVLKQIPVSEVWGIGQAYSDILTKKGMQTV
                     LDFKNADEHWVRRQMHVVGHKTLLELRGISCIPLESMMPDKKNICISRSFGSPLQQYE
                     DLEQAITTFAARAAEKLRQQQSCARGVLVFVMTNRFSSDPKYVKSTLLQMPVSTNITA
                     EIIHQTLKGLKKIYRKGYLYKKCGVILTDIIPDNQLQYTLWDNTNREKLCQLMETVDK
                     INGRIGQGAIKFAVQGTKRRWKMHQERLSPHYTSKWNDILTIKI"
     gene            11680..>12472
                     /locus_tag="PLI16_00840"
     CDS             11680..>12472
                     /locus_tag="PLI16_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83135.1"
                     /translation="MKKIKNFFLLLVIILSANSVYGQPGCTGVTINTNNGADITLPCN
                     QPCVTLNATAIQTSGQTATYTVSSIPYAPPFPFTGGTAIPITSDDDWSNVINIPNFHF
                     CFFTIPYDNLIIGDNGVISFDVGDAGGYCPWSFTSGVPHDYGSPMNIFGAFHDTYLPA
                     GGQMFYSVEGTTPCRSFVFKFNNVSQYSCNSLHTTQEIVLYETTNVIDVYINSKPLCS
                     SWNGGNAVVGIQNMAQTVGYAAPGRNSGQWTGTNEAWRFTPVGTSY"
BASE COUNT         3587 a         2768 c         2627 g         3490 t
ORIGIN      
        1 cttctctttt catcaccttg tggcattccg gacatttttc ccatcttgtt ttcggacatt
       61 cgtcgccgcc tatatgaatg tatttgccgg ggaacaactc cataacctct gccaatacat
      121 ctttcagaaa acgaatggtt ttgtctttgg tgcaaaatac atcatcaaat acaccccatg
      181 agcctgcggc ttcaaaaggc ccgccggtgc aagaaaattc ggggtaggcg cttaatgccc
      241 ccagggcgtg tccgggcatt tcaatctcgg gcactatttg cacatgccgg cgtgttgcat
      301 aggcaatcac ttcacgaatt tgttccttgg tataaaaacc gccatagcgt atggagttat
      361 atcgctgtac ggtgtcgtag gcgtgaccga taagggttct gttcctgtaa ccgcctatgc
      421 tggtcagttt tggatacttg tcgatttgta tgcgccatcc ctggtcatca gtaagatgcc
      481 agtgaaagac attcattttg taaaaagcaa gcaggtcgat atactttttt acttcttcaa
      541 cagtgaaaaa gtgccggcac acatcaaggt gcataccgcg ccaggagtag gcagggtagt
      601 cgtaaatact aaggttcctt atcttgataa tctctgtttt gtaaggaggc agcaaatgtt
      661 tgatcgtttg caaggcataa aaaacaccgg cgtcccggcc ttcaataaca ataaaagcat
      721 tggaaatatt cattttataa gcatcctgcg gcaactcttt gttggcagtt ttttttatca
      781 caatggaatt gctgtcgtac ttctggcttt ggtttataga taatgaaaag ccataatttt
      841 ctgtaaggta ttcattaaat aaggcagcgt ctttgcccga agtttccatg tcgtcaacat
      901 aaatctttgt ttgcgggcta aggataaaac gtcccttgga gtatgttatt tcaacggggt
      961 cagggaaaat ggaaacaatt tgtttctgcc cggaaaggca caccggaaaa agaatagtga
     1021 cgcaaagaag taataaattt aggatggttt tcatatgagc tttttaaaaa cacgcttatt
     1081 tttttggtaa tgtcgcacta ttttgccaat cactgtttcc acaccagaga agcggccccc
     1141 aatattgcgg catcattttc cttaatgccc gaagacaata tttttacttt atttttatat
     1201 atcggtaaaa gatttttttc aaaactttct ttcagcggtc taaaaagtat ttcgccggca
     1261 ttggctatgc cgccaaaaat aaatatggct tcaggactgg tatgtgccac actgttcgac
     1321 aatcctagtc ccagaatttc accggtatat tcgtaagtgt cgatggcggt ctggtcgcct
     1381 ttttctgcca gtttagaaat agtatagggt gtcattttac catccggcag tttcccttct
     1441 ttagccagca actcggcgta tgttttcaca atgccgcctg ccgagcaata tgcttcgagg
     1501 catccctgcc gtccgcagcc gcacacacgt ccccccggaa acacaatagt atgacctatt
     1561 tcgccggcaa agccgtcgtg cccgtacacc atatttccat ccaccacgat gccgcttcct
     1621 acgccggtac ccagcgtaat ttcaataaaa tttttcattc ctttggcatt gccgtaaacc
     1681 atttcaccaa tggcggcagc attggcatcg tttgtaagta tcaccgtgac atcgaaatgt
     1741 tgccggaaca agccaacaag aggcacaacg cccttccatt tcaggttggg agcaaattca
     1801 atactgccgt tgtaatagtt accgttgggc gcgcctatgc cgatcccgat gagggtggca
     1861 tctgtattgg aaagaaaaaa accttttaca atccgggaca cctcggtaac aaaaaccgct
     1921 ggatcatcaa atccgggtgt gctgatggta gttttatata aacagttccc gtcgctatct
     1981 acaaagcctg ttacggtatt ggttccgcca atatcaattc ctgctgcgat ttttttcata
     2041 atgattccgt ttttaatgat ttctttttct gatatccttt taatccgtaa aaaagtaagt
     2101 acaaatatga aaacatggga actacaaagg atgcgtgtac ccctaaatta tccgcagtaa
     2161 gcccttgcag cggcggaagc aatgcacctc caagtatcat catcaccagc aacgaagatc
     2221 cctgggatgt gtagcgaccc aaaccatcga tggccagggt aaaaatgttc gaccacatga
     2281 tggaattaaa cagcccgatg ccaagtacac accacatggc gatagagcca ttggtattaa
     2341 ccgctgccag caaaaggcaa aatgcaacaa ccgaaaaata ggccagcgtg cgtgcaggta
     2401 atgagcgccc gagcataaac aaacaataat tggcaacaat aaagatcagg aaataccata
     2461 ccctatcgaa agtcagcccc ttcatgtacc aggttatcag aaatatcacc agaaatgtga
     2521 gcaccgccat caataacatc agtaaatatt ttccggtttt tttcatgtta ctcagcgaaa
     2581 tagccccgag aaaacggcca atcatggcgc cgccccagta ataagccagg tagttgctgg
     2641 cttctacttc actaaaaccg acaatttccg gcagctttaa aaacgaaaca aggttactgc
     2701 caatggtcac ttctgcccct acataaaaaa aaatggcaaa cattccaaaa acaagatttg
     2761 gatgctttaa tgcgcctgct tttttttcga tgtgttcatg gctgttgagt gcaggaagct
     2821 ttgaaaacca gataaaaatc atgagcagaa tcaacacact gccaaggatc aggtagggaa
     2881 ccttcaccga ctctgctccc acattggctt ttgatgcaaa atattcaaaa ataaaaaagc
     2941 cgcctaaaac aggcgcaagt gtagtcccca aagaattaaa agcctgcgat aggtttaacc
     3001 ggctcgaggc cgtttcgggc gaacccaata ctgccacata aggattggcg gcaatctgca
     3061 aaacggtaaa gccaatgccg agcaaaaaca gggcgaagag aaaaaaaccg aacgaatgaa
     3121 acgtgttggc tgcaggataa aacaaaaaac aagcaatggc agatatagca agccctgtaa
     3181 gaatcccgtt tttgtagccg attttattta tcggatcgcc aaagctgagt gaaatgagaa
     3241 aataaatcag cgaaccaatg aaatatgccc caaaaaacgc aaactgtaca agattggcct
     3301 gaaagtgtgt caattcaaaa acgcctttca ggtaaggtat taaaatgtca tttaataccg
     3361 taataaaacc ccacataaaa aacagcgtgg tcagcgctat catagggtat gtgtattttg
     3421 tccgttgttc agtttccatt acttgcggag attaccctgt tagtatttac gtaaactatg
     3481 aacggtcgtt agatgctttc tttcgatttc atcagtgctt tcaccggcgc caggccaagc
     3541 acattgcctt cctgtatcgc cttgagcacg gctcccccgc cggtgaaaat atagtacttc
     3601 gggctgtcga gggccacaat atacaggccg ggcaataaac gtttcaactc ttgcagggtg
     3661 tcgccaccac cataaagttt tacggcatta ttgtttttat caatcagctc gtcgagcgcg
     3721 atagtacctt cattgaagtg gggagtaaag cccatcacgg cgttcacaaa aatagttttg
     3781 gcttctaaaa acactttttt aacttcggga tcctgaaacg attttgggtc cacatccagc
     3841 acataattca atttggttcc ggctttgatg tctttgatgt tatgcacacg gtattgtccg
     3901 gggcgcagtc cttccatggt gtcggactcg acgatataag ggagttcaat caattttccc
     3961 ggaaattgtg ctgcatattc cacaaacttt ttggccagtt ccatatcttc aggttctatg
     4021 ccttcgatgg tgaatccata tttggcacac agaaacgcgt tatagataac accacccaac
     4081 accagatgat ccgacacctt gagtaaggca ttcagcgagt caaccttggt gtcgaatttt
     4141 gagccggcaa ccacggaaac aaaaggttga tcgggtttat aaattcgttg caggttggaa
     4201 atttctttct gtaacaggaa tccggcatag gcgggaaggt atttagccac attaactgtg
     4261 gaagcgtgtg cctgccacga gccaaaagca tcattgacaa aaatatcggc cagtccggca
     4321 agctggtagg caaaacgatc ggcttccgca cctttggctt cttctccaaa aaaccaacgg
     4381 gtgttgggca ggtagatcat atcgatagta tggtcgttca ggtcgcgtat caggtgattg
     4441 attgatgttt ccacgtcgag gtagccgtgg gttccatact gctgaaattc gggcaccttg
     4501 attgtaatgt gcagtttgtt ttgcaggtag tcaacaatag gttgtacgga ggtgtcgtca
     4561 gaaacggcta tttcgccggt ttttttgttt tttggcctgc cggcgtgcgt catcaggata
     4621 atttttccgc ccttggcgtt gatataatat aaggtcgaaa tagtggcgtc aatcctgtat
     4681 ggatcgtgaa tgataccttt ttttaccaca ttgtgatcga cacgtaccag aactaccttg
     4741 ttttttaaat cggcgtcctg aagcagagga agattgatgc tctctaatat ttcattcata
     4801 gctgttatat tttaaaattt tatttaatgg ttttcttgtt ttttctctta ttctggaagt
     4861 tgaataaccc agtgtaatca gcaggcccac acgtttgttt ttggggatat ttagcagttt
     4921 catgacgttt ttttcgttga accagcccag tatgcatgta cccaggcctt cttcggccgc
     4981 ctgcaggcaa aaatgttcgg cagccatgcc gatatcgaag agataaaaat ctttgtttcg
     5041 tatgcggctg ccaaatcgcg acaacaagga tggtttttcg gttactatgg ccaccattac
     5101 cggcgcctgc agggcaaacc tgttaaaggg agatacggag ctgaaagttt ccccggctat
     5161 cttttgcttc aattccggct gatcagcaac cacaaaggtc catggctgtg aattacaggc
     5221 cgaaggtgcc agacgggctg cctcaatgca gcgcgctatc ttttcccgct ccacgagccg
     5281 gtcttcgtat ttccggctgc tttgccgaag ctttgtcaat tccagaaaat tcattttact
     5341 tctttggttt taatttttgc acgtacccat tgcttcagca gttcaaactc ttcgtccgaa
     5401 aaatattttt ttgggaataa attaaaccgg aacttattag gatacaccat gataatttcg
     5461 gatgtaataa gagccgactg ataaaactcc cacttgtttg tattgttaat attatcggaa
     5521 gacacctgta tctgttccgg cgaaaaaaca tagctgaaag gtttggcaaa atccggcatt
     5581 ttcgagaaca tccttttgcc cagattgtgc tgattgtaaa agtacagggc cgctattgcc
     5641 acgccgtaaa caataaggaa ccaggcggcc cagttggtgt aatcgacact gccgtttttg
     5701 tgttcataaa ataacagtat gcacccgata ataaatgaaa cggccgcaat aaccaataac
     5761 atacggctgc gtacaggata catttttttc aggtgtgtgg agtacgccat ttgcaggtct
     5821 tcgggtgtat agtgtatttt tttttctatt tgctccatgc ttatcaaatt aataaaaaag
     5881 aggcagatcg ttatgatcta cctctgcaaa tatatagatt ttgattgaag attgctattt
     5941 ctgtcagtta ctttcaataa tctttagcag ctcgtccaat tttggtgtca gtatgatttc
     6001 ggtacggcgg tttttgctgc gggcttcaga ggtactggct ttgtccacgg ggaaatattc
     6061 gctgcgaccg gctgctgtga tgcgcaccgg ggctatgccc gagttggcga gcaggatacg
     6121 gaccacggtg gtggcgcgtt ttacgctgag gtcccaattg tctaaaagct ggtcagttcc
     6181 tttgtagggt acattgtcgg tgtggccttc caccatgata tttatatcca cattggattc
     6241 cagcactttt gccagttttt tcaaagcatc agcgcctttc gaagccacct cgtacctgcc
     6301 ggaggcaaaa agcagggctt catccatcga aacgtaaacc ttcccattca tttgctttac
     6361 cgtcaaaccg ttgttttcgt atccgagcaa ggcttccgaa actttgtttt tcagtgcttt
     6421 taccgccgaa tcttttttgt ccagtatggt ctgcagttcg ttgagtttct gttgtttttc
     6481 gtcaagtttc aactgcatct cgttgaggtt ggcgagtttg gtgtttaatt ccgattccag
     6541 ttttttcagg tcgtcttctt tcttttgtaa agtttctttg gttttttcca gctctgaaat
     6601 cagtttttgc gtttcaaatt tgttgccttt cagcagtttg aggttgttat tttgtaaggt
     6661 gtcgtaagcc atactcagtt cctgatattt atgcgtaaga tcccgaagct tcataccctt
     6721 gtttatggaa tcggcaacaa gcagctcgtt ttgttttttc aacttatcag ccgtggccgt
     6781 gagttcggta agcctggtaa catactccat cactttgcct ttcagggttt tgttttcttc
     6841 catgcagttt ttttgattgt tttctgcttc ctgaaatttt ttcagggata cgcacgacga
     6901 gagaaacagg cagcaaagcg caaacaaaat aaccggtttc agttttttca tgtctgaatt
     6961 tttttcaaaa gtacacttat taaatgtgtt caaagcctca atcttcgcaa gatactaaca
     7021 cactgctgtt aaaaatgcgg aggctgtttt gtgaagcttt gtccccgggg gcttatcctg
     7081 acgggagtta tcaccggggt tgcctgacct gctgcccgca acgtccacac acattcacag
     7141 attttgcttc gttaaattat gataggtagg gcgcaaaagg atcagaaatc tacttcaagt
     7201 atgctcggac agtggtcgga atgtttggct tccggcaaaa tcactgccct tttcataaga
     7261 tgcctgatgg gttcgcttac catgtggtaa tcgatgcgcc agcccaggtt tttggcacgg
     7321 gcattagccc tgaaactcca ccaggtgtat tgttttggct cgttgttaaa cgccctgaac
     7381 ccgtccacaa atcccgaatc aatgaatttc gacagccact cgcgttcttc gggcaaaaaa
     7441 cccgaactgg tagcattgcg tatagggtcg tggatgtcga tcggcttgtg acagatattg
     7501 tagtcgccgc aaataatgag gttagggcgt gattttttca attcgttaac ccagtccaga
     7561 aagtcgtcaa gccacaccat tttaaaagcc tggcggtcat cgccgctgga gccggaagga
     7621 tgatacacgc tgagtacaga aatatcgcca taatcggcac gcaacacgcg gccttcgtca
     7681 tcatattttg gaatacccat gccataaacc acatggtcgg gttcttgccg ggtaataatt
     7741 ccaacaccgc tgtatccttt cttttttgcc gaaaaccagt aattatgata ccccagtgca
     7801 ttaaactcca atgtcggaat ctgttcgggc tgtgccttgg tttcctgaaa acacacaata
     7861 tcggcattta ccgaagcaat ccagtcgagc agaccttttg atgtggctgc acgtatccca
     7921 tttacgttat aggtaacgat ttttttcatc agtccagaat tttgttggct aaactaatga
     7981 ttttgccttt aaatacgaaa aattaaaaaa ataaatcttc tttggctttt ttttatagca
     8041 ccacggcagt tatttggtaa tagcagcaac ccgttgtcat ggacttcttt ttacatcggt
     8101 tccttacggt atttaataat aaaaacttaa atttgcatat caatctcccg aaaattggct
     8161 ctctttctta aaaaaaaatc cattgagtta cctgacccac taactgacca ggagttgata
     8221 gaattgtatc gccaaaccgg tgacaccgct cacatcggcg aacttttcga ccgctatgcc
     8281 catttggttt acggtatcgc attaaagtat ttcaaccagg aagacgaccg ccgtgaagcc
     8341 gtcatgcaga tttttgaaaa attatacgtg gcattaaaag accatgagat agaaaacttt
     8401 aaatgctggt tgtacagcgt ggcaaaaaat cactgcctga tgattttacg caaagaaaac
     8461 tccgtgtctt tcaacgaaca acttcagctg aaaaattttc aacaggattt tatggatttt
     8521 gacgggaatt tttctcttaa tcatgctgaa gacgaaatta ttgacaaaaa attgcccgag
     8581 gccatcaaaa aactgagtga tgaacaacgt atctgcatcg aattatttta cttgcatgag
     8641 aaatcgtaca aggaagtggc tgaaacaact caatactcta tgaatcaggt aaaaagcttt
     8701 atacaaaacg gaaaaagaaa tttaaaactc atgctcgaaa acataaaatg aaacgttcag
     8761 acaaacggaa ccggatattt ggtccttcgg gatgtatcag cgaagagggg atttccctct
     8821 acctgaaaaa ccaactgcct gcaaaagaaa aaaatctttt ggaacagcac ctggccggct
     8881 gtttattatg caatgaagcg atggaagctt atgaaaaatt ttatgaacac ggaaagactg
     8941 cagatgattt atttgcaata aaatcttcat tgaaatcaac gtattcaaat aaaacagcaa
     9001 acaaaagaaa cagccaaagg acactcatca caaccttatc catcgccgcc agtatcattt
     9061 tgatttttac ggggtactat tttattaatc tgcaaaccaa aaacgagaat aaagaaatca
     9121 gttatactgc accgaaaaat cttgagaaac caattgttgg atcagagcaa aaaaacccgc
     9181 aggaattaat aaaccaacct gccgaaaaaa cctcagtgaa ggaagacaaa acgaaaagca
     9241 taccttctca ggccccacaa aatgccgact acttgtataa gaccacaacg ggtactgaca
     9301 gagaaacatc cgtcgcagcc acgggaacgg caaaggttgt tgcagacgag gccccgggtt
     9361 attttgccga caccccggat aaaaaagaag aataccgcaa cgacatttat gcaggagatt
     9421 atgatcaggg ccttgttctt ggcggtgtag caactgctga aagcaaggcg gacacaaaaa
     9481 atgccaaagc tgtcaaccag gaaatagccg tatcttcggc gcctttggaa gcagaaaaag
     9541 ctgtttctga aaaagacctt gcgctggaat catatgaaaa tgccgttgat aaagtggtgc
     9601 tttcagggaa aaaaactgcc agaaaagaag taagtcgctc acagattaaa acaccggcta
     9661 atacagcatt atcgtattat tattcaggac aatacaaaaa agccatcccg gaattcaata
     9721 aaaaggttgc ccgaaacagc caggattatg aatctatcta ttacttagcc atgtcttatt
     9781 actacgaagg gcaaaaagac gctgccttgc ctcttcttga tacgatactg aaaaagaaca
     9841 gcaatcctta ctacgatctg gcattatggc aaaaagcaat catcctcacc gaaaaaaagc
     9901 agactgccga agcccgcacc ctgctgaacg aacttgtcga ccgcggcggc aaccttaaaa
     9961 atgatgcttt acaaaaatta gatgaacttg ataaggccga ataaggactt ccccaaaccg
    10021 gtgttgaaca aggtatttgc cctggttgat tgcaacaatt tttacgcttc ctgcgaacgc
    10081 ctgtttaatc ccaagcttga aaaaaaaccg cttattgtcc tctcaaacaa tgatggctgt
    10141 gtgattgccc gctccaacga agcaaaatcc attggtatta aaatgggcgc acccgctttt
    10201 gaatttaaag aattgattgc ccgtcatggg gttcatgttt tttcttcaaa ttatgccttg
    10261 tatggcgata tttcaaacag aatcatggcc atactgttcg aaatggcacc ggaggtggag
    10321 gtatattctg ttgatgaggc ttttctgaat ctcagcggga tggatcctga ggctattaac
    10381 ctgttagctg caaatatccg caaaaccatt aaaaaatggg tgggaatacc agtttcggtg
    10441 ggtattggcc caaccaaaac cctggcaaaa attgccaacc atctggcaaa aaaaaatccg
    10501 gcctatgcca atgttgcaaa cttgtgttcc attgaaaaca ccgatgacgt gttgaaacaa
    10561 attcccgtgt cggaagtttg gggtatcggt caggcctaca gtgatatatt gacaaaaaaa
    10621 ggcatgcaaa cggtacttga tttcaaaaat gcggatgaac actgggtgcg caggcaaatg
    10681 catgtagtgg gccataaaac cctgcttgag ttaaggggca tatcctgcat tccactcgaa
    10741 tcgatgatgc ccgacaaaaa aaacatctgt atctcacgct cttttggatc tccgcttcaa
    10801 caatacgaag acctcgaaca ggccattacc acctttgccg caagggccgc cgaaaagttg
    10861 cgtcagcagc aatcctgcgc caggggagta ctggtttttg tgatgaccaa caggttttca
    10921 tcagatccga aatacgtgaa atccaccctg ttgcaaatgc ctgtatctac taacatcacc
    10981 gccgaaatta ttcatcagac actaaaaggc ttgaaaaaaa tctacaggaa aggttaccta
    11041 tacaaaaagt gcggagtcat tcttaccgat attataccgg ataatcaact ccaatatacc
    11101 ctttgggaca acaccaatcg cgaaaaatta tgtcaactta tggagaccgt cgataaaatt
    11161 aatggaagga taggccaggg ggcaataaaa tttgcagtgc agggcacaaa acggcgctgg
    11221 aaaatgcacc aggaacggtt atctccgcat tatacaagca aatggaacga catcctaaca
    11281 ataaaaatat aaaattatat tgctatttaa gtgcctgatt tttcagcaaa aacctgtatt
    11341 tcaatcgata ataggcgatt ataataaact ataagttcac ttaaaaagaa tttttatgtg
    11401 aaaataagct ttgtaccctc cccaaagcca gcctgtgtgc cacaaacagt agaaggcagg
    11461 ggtgactgat tttcagcggc ttcccttggg agtcaagggt aatactcaat taaaatataa
    11521 agtacttttt gctattgcaa tataatattg tatttttgct gataaatgca acctttagca
    11581 cgaaaaattc gtctaaatag ttcaaaatgt ttttatcaac caagtactaa agacaaaaaa
    11641 tgcgttttat taacaaaact tgttaattat caacaattga tgaaaaagat taaaaacttc
    11701 ttcctgcttc ttgtcattat cttatcggcg aactcagttt atggacagcc gggatgtacc
    11761 ggggttacta taaacacgaa caatggagcc gatattacgt taccgtgtaa tcagccctgc
    11821 gtaacgttga atgcaacggc cattcaaacc agtggtcaaa cagccaccta taccgtttct
    11881 tcaatacctt atgcaccacc tttccctttt accggcggta cggccattcc tatcacctcg
    11941 gatgacgact ggagtaatgt gattaatatc cccaattttc atttctgttt ctttactata
    12001 ccttacgaca acctgattat tggcgataac ggggtgattt cttttgatgt gggcgatgcc
    12061 ggcggatatt gtccctggtc attcacgagc ggggttcctc acgattatgg ttctcccatg
    12121 aatatattcg gcgcttttca cgatacttat cttccggcag gagggcagat gttttattct
    12181 gtagaaggca caacgccttg ccgttccttt gtatttaaat tcaacaacgt ttcgcaatac
    12241 tcgtgcaaca gccttcacac cacccaggag attgttcttt atgaaacaac caacgtgatt
    12301 gatgtgtata tcaattccaa gcccttatgt tcaagctgga atggaggcaa tgccgtggtg
    12361 ggcatacaaa atatggcgca aaccgtgggt tatgcggcac cgggcagaaa ctccggacaa
    12421 tggaccggca ccaacgaggc ctggcgtttt actccagtag gtacctctta tg
//
LOCUS       DAOSFP010000013        11888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4539_length_11888_cov_4.244401, whole genome shotgun sequence.
ACCESSION   DAOSFP010000013 DAOSFP010000000
VERSION     DAOSFP010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11888
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4539_length_11888_cov_4.244401"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLI16_00845"
     CDS             complement(<1..584)
                     /locus_tag="PLI16_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83136.1"
                     /translation="MKKFITCYGSLIKVENLWCLSELGIPQTCLLESLDAFPGYYGDS
                     PKINVPRFVYFVLGKPYSMEQITRVSQQIKKSITYEFDAAFCEISMRDNTCCAIRITG
                     LTDYSLIKSLQEAYRDKGMLLKKKIKNIENEPGLINIRKFFRLEQCPSGVYLDKELTY
                     NSYFVIPGEISWETLKQHIDLLRNNWNQGSFDAA"
     gene            complement(854..1300)
                     /locus_tag="PLI16_00850"
     CDS             complement(854..1300)
                     /locus_tag="PLI16_00850"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOH83137.1"
                     /translation="MSKIRLTKEFKFEMAHALQGYEGPCRNIHGHSYYLYVTVIGEPV
                     CDHNSPVNGMVMDFTELKNIVRTEITDQLDHALVLSDKIPPQLLAALKGFNNIILLPY
                     QPTCENMLQDFAGRLQKKLPAHIRLFSLKLCETVTSSAEWYASDND"
     gene            complement(1304..1861)
                     /locus_tag="PLI16_00855"
     CDS             complement(1304..1861)
                     /locus_tag="PLI16_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOH83138.1"
                     /translation="MNITEIVLIALGLAMDCFAVSISCSLGKNNISRLLAVKIALFFG
                     LFQGLMPVAGWLLGLSFKNMMSGFDHWIAFGLLSIIGIKMIVEAFKKEEERDLNIRKT
                     MVLISLSVATSIDAMVVGISFALLKLNMLLTLGIIGIVTFIVSLVGIYIGKKFTFISA
                     GKAAIIGGLVLIGIGTKILIEHLNP"
     gene            2005..2454
                     /locus_tag="PLI16_00860"
     CDS             2005..2454
                     /locus_tag="PLI16_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83139.1"
                     /translation="MLEELLKLVKENAGDAIINNPAIPNKKNDAVCKAATKSLMDKMK
                     GIAGTEGMDSITKLFNEGGDLNNNTTINNISDGVAGDLMKKFKLDGNVAGTLVKSLIP
                     VVLGKLISKTNDPNDNSFSLESVIGMLAGGSGAGGFLGKLKSIFGGK"
     gene            2598..4346
                     /locus_tag="PLI16_00865"
     CDS             2598..4346
                     /locus_tag="PLI16_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOH83140.1"
                     /translation="MKIFYIAFLFLLASLEAVSQTWQQAYVNEDAMVRCQRVNAPRIA
                     HTKDSYPQMAGFPVSVAGNPYFKNFRGLALEDMDTNGITDIVFAANSTLYVYSYQGLI
                     WQKAITGTAVYPPSVSDINNDGLPEIVQVTGGSPDNGRIYAFDHDGNIIPGWPVNFSN
                     HWIICSPVLSDLNNDGNKEIIVCERMSPAGRVHILSNDGTSFSANWPVTLDGIPAVTP
                     SVADVDSDGEKEIVAYSTKSRYVFALDGTPETGFPLVTDPLQKYSYQSPVIVDLDNDG
                     QCEVAGATHGDAPEFYVMKNDGSAYPGWPKAVTDNSWTYSPPTAVRINGEWNIFMSRP
                     IGSDADEMLYGWNAAGNLLPNFPVVKPGGLEGFISVADINNDGSFELVFGSNLLDSTG
                     YGFIHAYNISSGGQVSGFPIRPRGWTFMNGANVGDVNGDNMMDLVALSYTNSYDSLFP
                     DKVYVNVYELNVPYAPEKVLWGTYKGANERTGYAGEMISGLPQPLPLVQNISVSPNPV
                     QEMLTLTWDQEAGGSMFLSIRDMAGRTLRNYEFSGLPAGANAITVDMSTLPAGCYFSV
                     LSGEEGVSRVTKIIKN"
     gene            complement(4356..5738)
                     /locus_tag="PLI16_00870"
     CDS             complement(4356..5738)
                     /locus_tag="PLI16_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOH83141.1"
                     /translation="MELPFTESYKIKMVETIRRSTKEERLGWIREANYNLFNLRSEQV
                     FIDMLTDSGTGAMSDKQWSAMMVGDESYAGASSYYNLKDAIKNIMGFDYFLPTHQGRA
                     AENVLFSVLVKEGDLVPGNSHFDTTKGHIEFRRATAVDCTIDEAFDTTLIHPFKGNVD
                     LNKLETVLKNNPIEKIPFIIVTVTCNSSGGQPVSMANLRDVHSLAQKYGIPVVFDSAR
                     FAENAYFIKIREKGYENKTIKEIVREMYSYADVATMSSKKDAIVNMGGFIAMKSKELF
                     QKASFYNIMFEGFLTYGGMSGRDMNALAQGLDEGTEFDYLDTRIKQIAYLGQKLKDYG
                     VPLQEPFGGHAIFVDAKKFLSHVPKEEFQAQTLAVELYIEGGVRGVEIGAIMTDPDPV
                     THQPRYPKLELVRLAVPRRVYTNNHMDYVAAALGNVYERRMGIKHGYRITYELPIMRH
                     FTVGLEKIEA"
     gene            6167..6871
                     /locus_tag="PLI16_00875"
     CDS             6167..6871
                     /locus_tag="PLI16_00875"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOH83142.1"
                     /translation="MGNILLQYMVNRFNAGAEGIKPPVANLPFITLSREYGCPAQDVA
                     VMLVDRLNNANEKTKNLPPWTKLSKEILLAASQELHVDPSRIYKIFNDEKRGAIDEIL
                     NAFSEKYYYSDLKILKTLDKIILDFATRGNVVIIGRGGMAVTRHLSAGVHVRLFAPIE
                     WRVKKLIESGACTTIEAAKKLSNQIDFKRNVLLKSKSKSDNYEDDFDVFYNCKYLGAE
                     DISESIIQLMKLKKLL"
     gene            6948..8303
                     /locus_tag="PLI16_00880"
     CDS             6948..8303
                     /locus_tag="PLI16_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOH83143.1"
                     /translation="MNIHFIAIGGSVMHNMALAMVQNGHRVTGSDDEIFEPAKSRLAA
                     KGILPASPGWFPDKINSGLDAVILGMHAREDNPELIKAKELGLKIYSFPEYIYEQSKN
                     KTRVVIGGSHGKTTITAMIMHVLHELGKDFDYMVGAQLEGFETMFRISDAPVIILEGD
                     EYLTSTLDKRPKFHLYRPNIALLSGVAWDHINVFPTFEIYVDQFRKFIELIEPDGSIV
                     YCENDEELKCICPEARKDIKLLPYGIPQHSINNGTTSVAIGDKATDIRVFGKHNLMNL
                     NGARLVCECLGISTTDFMRAIAGFKGAAKRLELLGKNEDSAFYKDFAHSPSKLRATID
                     AVKEQFPQRELVACMELHTYSSLNSDFLEQYKGCMQKADKAIVFYSAHALEIKKMPPL
                     PENKVKGSFGRDDITVFTNKEKLVEYLESLDWKGKNLLMMSSGSFDGLDLLEFVKNLE
                     I"
     gene            complement(8442..9308)
                     /locus_tag="PLI16_00885"
     CDS             complement(8442..9308)
                     /locus_tag="PLI16_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH83144.1"
                     /translation="MQSKIIVITGASDGIGKETAKTLAKQGHTLIIHGRNKQKLQAVY
                     DEIKAESGNSNIDMFTADFLSLAEVKRFADAIKEKYDHIDVLINNAGAQYTAKRETTP
                     EGHEKTMTINLLAPFLLTHLLLDMLKKSKSARVVTVSSAAHKMGGKPYLDDIELKNNY
                     TMPKAYGLSKLYVIWIMRHFVAEMISAGIENITFNTVHPGSAITSLGRESMKSLKWKI
                     IGILWLPMMNSVAKGASSSIKTATAPEKEGVTGRYFGPKGEEKPSEKYYSIENEKTVW
                     NYCMEAAKPYIN"
     gene            complement(9337..9999)
                     /locus_tag="PLI16_00890"
     CDS             complement(9337..9999)
                     /locus_tag="PLI16_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83145.1"
                     /translation="MKVIITGTTGMIGEGVLIESLTHPSITEVLSVSRKPTGKSHPKL
                     KEYIVPDFLTLKEDDEKLQGYDACFFCAGISSVGKKEEEYTKITYDTTLHFAKALNPN
                     PAMSFIYVSGGGTDSTEKGRMMWARVKGKTENDLMKLPFKQAFGYRIGFVKPVAGQVH
                     VLKYYPYISWLFPVIRILFPFMYSTMKQVSQSMIYVAQNSYKQNVIYVKDIKDLAKRT
                     IK"
     gene            10117..10491
                     /locus_tag="PLI16_00895"
     CDS             10117..10491
                     /locus_tag="PLI16_00895"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOH83146.1"
                     /translation="MLINELSKKTGVSIHTLRYYENLGLIQGVSDEKVTTNNYKNYDE
                     IQVERVEIIKEAKEVGFTLSEIKKMLDSWYSGLLTPREQVKMFEAKVNDIEIKIRHLK
                     QVKKRLEKMIREVEKGVNCQPK"
     gene            10786..>11888
                     /locus_tag="PLI16_00900"
     CDS             10786..>11888
                     /locus_tag="PLI16_00900"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH83147.1"
                     /translation="MVFKSEIESAYNLQQSDVKIKKSGVKRAYLSKLLPESKHIEVIS
                     GIRRCGKSTLMKQLMQQYKTDIAYFNFEDPRVNNFEIGDFQKLDEIIGRNKAAYFFDE
                     IQDVASWEIFVRQLHDRGEKIYITGSNASLLSKELGTRLTGRHLRHELFPFSYLEFLE
                     YKNLKNNENSFEKYIQKGGFPEFLREENQEILQSLLKDIILRDIAVRYGIKNSKTLMD
                     ITLFLISNIGKETTYNSLRKSFSVGSANTVSDYLSWLEDTYILFFLPRFSWSAKSIAV
                     NPRKVYAIDNGLAIANSLSFTNDKGRLLENAVYLFLRQKFNSLYYFRENSECDFVVFG
                     KGKCLLLIQVCEQVHNENKDREVNGLLDAMRFF"
BASE COUNT         3350 a         2421 c         2604 g         3513 t
ORIGIN      
        1 tttgcggcat caaaactgcc ctgattccag ttgttgcgta gcaggtcgat gtgttgtttt
       61 aaggtctccc agctaatttc cccgggaatc acaaaatagg aattataggt cagttccttg
      121 tcgagataaa ctcctgaagg acattgttca agcctgaaaa attttctgat gtttatcagg
      181 ccaggttcat tttcaatgtt tttgattttt ttcttcagca acataccttt atccctgtat
      241 gcttcctgaa gtgatttaat cagcgaataa tccgtaaggc ctgtgatgcg tatggcacaa
      301 cacgtgttgt cccgcatgga tatttcgcaa aaagcggcat caaattcgta agttatgctt
      361 ttttttatct gctgcgagac cctggttatc tgttccatcg aatagggttt acccaacaca
      421 aaatatacaa accttgggac attgatttta gggctgtctc cgtaatagcc cggaaaagcg
      481 tccagcgatt cgagcaggca ggtctgcggt atgccaagtt cgctgaggca ccacaaattt
      541 tcaaccttta taaggctgcc gtagcaggtt ataaatttct tcataatctt gttttttatg
      601 atgaaaccaa ttagtcaaag ataataataa ttttttaaaa ttcctagtaa ctggctacgt
      661 gtaacaggat ttaaagggat tagtgatatt ggtacaaact ggtgtttcct ccaaattaat
      721 cctgagtata atctggttag tgcaggacga aaggtaactg cctaaggaac aaaccaggtg
      781 caaaagtcca ctgtacggat gtaatcaaag gatgttgagc agggggcggg ttatttgcca
      841 gtaatggagt gggctaatcg ttgtcgctgg cataccattc agccgatgaa gttaccgttt
      901 cgcacagttt caggctgaat aagcgtatat gtgcaggtaa ttttttttga aggcggccgg
      961 caaagtcctg gagcatgttt tcgcaggtgg gttgataggg aaggagaatg atgttgttaa
     1021 aacctttcag cgccgcgagc agctgcggag gtatcttgtc agacaatacc agcgcgtggt
     1081 cgagctggtc ggtaatctct gttctcacaa tattttttaa ctcggtaaaa tccataacca
     1141 tgccgtttac cggcgagttg tggtcgcaca ccggctcgcc gatgacggta acatacaggt
     1201 aataggagtg tccgtggatg ttacggcatg ggccttcata accctgcagg gcatgtgcca
     1261 tctcgaattt gaattcttta gtgagcctga tttttgacat aatttatgga ttaaggtgtt
     1321 caatcagtat ttttgttcct atcccaatca gtacaaggcc tccgattatc gcagccttcc
     1381 cggcgctgat aaaagtaaat tttttcccga tataaattcc caccagcgaa acaataaagg
     1441 ttactatccc gataattccc agggtcagca acatgtttaa tttaagcaag gcaaaactta
     1501 tacctaccac catggcatca atgctggtgg ctacggataa acttatcagc accatggttt
     1561 tgcgaatgtt cagatcacgt tcttcttctt ttttgaaagc ttccactatc atttttatcc
     1621 cgataatgct cagcaatcca aaagcaatcc aatgatcaaa accactcatc atgtttttga
     1681 atgagagtcc cagcagccat cctgccacgg gcatcaagcc ctgaaacaac ccgaagaata
     1741 aagcaatttt tacggccagt agcctggaaa tgttgttttt gcccaggctg caacttatcg
     1801 acacggcaaa acaatccatt gccaggccaa gagcaatcag tactatttcg gtgatattca
     1861 taatgccgca aaattataaa tatttgcgat taccggcaca tttgtcaggg attatcccct
     1921 agttgtaaaa ataaatataa aaattattat tattatgttt ttgttgttat atttgtgaaa
     1981 taataaccct aaaaatgcca aattatgtta gaagaacttt taaaattggt aaaagaaaat
     2041 gcaggtgatg ccattattaa caaccctgcc atcccgaata aaaaaaatga cgctgtgtgt
     2101 aaagctgcta ccaaatccct gatggacaaa atgaaaggga tagccggtac cgaaggcatg
     2161 gacagcatta caaaactttt taacgaaggc ggcgatctca acaacaacac cacgatcaac
     2221 aatatttcgg acggcgtggc cggtgacctg atgaaaaagt tcaaactcga tggcaatgtg
     2281 gccggaacac tggtaaaatc gctgataccg gtggttttag ggaaattaat cagcaaaacc
     2341 aacgacccca acgacaacag ttttagcctg gaaagcgtta tcgggatgct ggcgggcggt
     2401 tccggcgccg gtggattttt gggaaaactg aaaagtattt tcggtggaaa gtagttcctt
     2461 tcctgacctc tgattaggat tgttactcat gccgttgcga cagcggcctg gtaacgtatt
     2521 accttcaggc attttttttg aatggtgagt gattttctca ggccttgtta tatctttgtt
     2581 ttccaaaaaa accgaaaatg aagatatttt acattgcgtt tttattttta ttggcttcac
     2641 tggaagctgt gtcgcaaaca tggcagcagg catacgttaa cgaagatgct atggtgcgct
     2701 gccaaagagt caacgccccg agaatagcac atacgaaaga ttcttatccc caaatggccg
     2761 gcttcccggt ttctgtagcc ggaaacccgt attttaaaaa tttccgcggc cttgccctcg
     2821 aagacatgga tacaaacggt atcaccgata ttgtttttgc cgctaacagt acgctttatg
     2881 tttattcata ccaggggctt atctggcaaa aagctattac cggcacggct gtatatccgc
     2941 cttcggtgtc ggatatcaac aacgacgggc ttcccgaaat tgttcaggtt accggaggca
     3001 gccccgacaa cggacggata tacgcctttg accatgacgg aaacattata cccgggtggc
     3061 ctgtaaactt cagcaatcat tggattattt gttcacccgt tctttctgac ctgaacaatg
     3121 atggcaataa agaaattatt gtctgcgaaa gaatgtcgcc tgccgggagg gtacatattc
     3181 tgagcaacga cggtacttcg tttagcgcca actggccggt taccttagat ggaatacctg
     3241 ccgtaacacc ctcggtggcc gatgtggata gcgatggcga aaaagaaatc gtggcttatt
     3301 ccaccaaaag ccgttatgtc tttgcgcttg acggcacgcc ggaaaccgga tttcctcttg
     3361 ttactgatcc cctgcaaaag tacagttacc aatcgcccgt tatcgttgac ctggacaatg
     3421 acggtcagtg cgaggtggcc ggagccaccc atggcgatgc cccggagttt tatgttatga
     3481 aaaatgatgg cagcgcatat cccggctggc ctaaggccgt tacagacaac agttggacct
     3541 attcgcctcc aaccgcagtg agaataaatg gtgaatggaa tattttcatg agccgcccca
     3601 ttggcagcga tgccgatgaa atgctttatg gatggaatgc cgccggcaac ctgctgccga
     3661 attttcctgt tgttaagcct ggcggcctgg agggatttat ttctgtggcc gatatcaaca
     3721 atgatggctc ctttgaactc gtgttcggct ccaatttgct ggattctacc ggctatggtt
     3781 tcattcatgc atataacatt tccagtggag gccaggtgtc cggatttccc atacgtccgc
     3841 gcgggtggac atttatgaac ggtgccaatg tcggggatgt caatggggac aacatgatgg
     3901 acctggtggc tctatcctat accaactctt acgatagcct gttccctgat aaagtgtatg
     3961 tcaatgtgta tgaattgaat gtgccttatg cccccgaaaa agtgctttgg ggtacttata
     4021 aaggcgctaa cgaacgcaca ggctatgcag gggaaatgat ctctggcctg ccacagccgt
     4081 tgccattggt gcaaaacatc agtgtttctc ccaatccggt gcaggagatg cttacgctga
     4141 catgggatca ggaggccgga ggcagtatgt ttttatccat ccgtgatatg gcaggccgta
     4201 cgctgcgaaa ctatgaattt tcggggctgc cggcgggcgc caatgctatt acggttgata
     4261 tgtcaacatt gccggcaggt tgttattttt cagtgctctc cggcgaagaa ggtgtttccc
     4321 gtgtaacaaa gattatcaaa aactaaagcg cgatcttacg cttcaatttt ttccagtcct
     4381 acggtaaaat ggcgcatgat gggtaattcg taagtgatgc ggtaaccgtg tttgattccc
     4441 atacgtcttt cgtaaacatt acccagtgca gctgccacgt aatccatgtg gttgttggta
     4501 taaaccctgc ggggtacagc cagccttact aattccagtt tcgggtagcg gggttggtgg
     4561 gtgacgggat cagggtccgt catgatggcg ccaatttcca caccacgtac gccaccttca
     4621 atatacagtt caacagccag cgtttgtgcc tgaaattctt ctttgggaac atgtgaaaga
     4681 aattttttgg catcaacaaa aatggcatgg cctccaaacg gttcctgcaa gggtactccg
     4741 taatccttta atttctgtcc taagtaagct atctgtttga tacgggtatc gaggtaatca
     4801 aattctgtcc cttcgtccag cccttgagcc aacgcgttca tgtcgcggcc cgacatccca
     4861 ccgtaagtaa gaaaaccttc aaacatgatg ttataaaaac tggctttttg aaacaattcc
     4921 ttgctcttca tggcgataaa accgcccatg ttgacaatag cgtccttttt actgctcatg
     4981 gtggctacgt cagcatagga gtacatttcc ctgacgattt ccttgatcgt tttgttttca
     5041 tagccttttt cacggatctt gataaaatat gcattttcgg caaagcgagc cgagtcaaaa
     5101 actacgggaa tgccatattt ttgtgccagg ctgtgcacat cgcgcaaatt ggccatagaa
     5161 accggctgcc cgcctgagct gttgcaggta acggtaacga tgataaacgg tattttttcg
     5221 atagggttgt ttttcaacac agtttcgagt ttgttcagat ctacattgcc tttaaaagga
     5281 tgtatcaggg tggtgtcaaa agcttcatca atagtgcagt ccacggcggt tgccctgcgg
     5341 aattctatat gtccttttgt ggtgtcgaaa tgcgaattgc ccggcaccag gtctccttcc
     5401 ttgaccaata ccgaaaacaa aacattttct gctgcgcgtc cctgatgcgt gggtaaaaaa
     5461 tagtcaaatc ccataatgtt tttaatggca tccttgaggt tataatacga agaagctccg
     5521 gcgtaacttt cgtcaccaac catcatggcc gaccattgtt tatcgctcat ggctccggtg
     5581 cccgaatctg taagcatatc aataaatacc tgctcgcttc gcaggttgaa aagattgtaa
     5641 ttagcctctc tgatccaccc caggcgttct tctttggtgc tgcggcgtat agtttcgacc
     5701 atcttgattt tgtacgattc tgtgaatggt agttccatag tttttattat atttaagaag
     5761 ttttaatttt ggtcaaaagt aaatattgtt ttattcagga aaaaattttt tgagaaaaaa
     5821 ctttccggcg atgtttttat tttgttaaga tatttgacct gaaaaattca tacgaatttt
     5881 tcaccttatg ggcaggcaaa agaaaatccg gtaatccggc aatgatttgt cgtttcataa
     5941 tttttctttg ccggggtata acatggcctg ctgcttgcaa cgtccacaca cattcatcaa
     6001 tttcacttcg ttcatgtatg aataggccag gttaatgaca aggttttgtt tctatttttt
     6061 ttgcagacac ttcatgaaaa ttataatgcg aaaattagga aattcatcag gatattagta
     6121 tctttatagc atatatcagt acattattaa aaaaaggagg cgcattatgg gcaatatact
     6181 tttacaatat atggtaaatc gtttcaatgc cggtgcagaa ggcattaagc cgccggttgc
     6241 gaacctgcct tttataacct tatccaggga gtatggctgt ccggctcagg atgtggcagt
     6301 gatgctggtt gatcggctta acaacgccaa tgaaaaaaca aaaaatctgc caccctggac
     6361 aaaattaagc aaggagatat tgttggctgc ctcccaggaa ttgcatgtgg acccgtcaag
     6421 gatttataaa atcttcaacg atgaaaaaag gggcgctatc gatgaaatat taaatgcttt
     6481 ttcagagaag tactattatt ccgacctgaa aatcctgaaa actctggata agattattct
     6541 cgatttcgcc acgcgaggca acgtggttat cattggcagg ggcggtatgg ctgtcacacg
     6601 ccacctgtcg gcgggcgtgc atgtgcgctt gtttgcaccc atagagtggc gagtaaaaaa
     6661 gctcatcgaa agcggtgcct gcaccaccat agaagcggct aaaaaattgt cgaaccagat
     6721 agatttcaaa agaaacgtcc tgctgaaaag caaatcgaaa agcgacaact acgaagacga
     6781 ttttgacgtc ttttataatt gtaaatacct gggtgctgaa gacatatctg aatccatcat
     6841 tcagttgatg aaactcaaaa agttgctgta gtttttccgc cgggagctgt tgaaaacttc
     6901 acttttgtgt tttgtgacag cccttctttt tagataattt tgttttcatg aacatacatt
     6961 ttattgcaat aggcggcagt gtcatgcata atatggcgct ggccatggtt cagaacggcc
     7021 atagggttac cggctctgac gatgagattt tcgaacctgc aaaaagccgg ctggcagcga
     7081 aagggatttt gcctgcatcg cccgggtggt tccctgataa aattaatagc ggccttgatg
     7141 ccgtgatact gggcatgcac gcgcgtgaag acaatcccga actgataaaa gcgaaagagc
     7201 tgggattgaa aatctattct ttccccgaat acatttacga gcaatcgaaa aataaaacac
     7261 gcgtggtaat tggcggcagc cacggcaaaa ccaccatcac cgctatgatc atgcatgtgc
     7321 tgcacgaact gggaaaagat ttcgattaca tggtgggcgc tcagctcgag ggttttgaaa
     7381 cgatgttccg catttccgat gcgccggtca ttattctgga aggcgacgaa tacctgactt
     7441 ccaccttgga caaacggccc aagtttcacc tgtaccggcc caatatcgcg ttgctcagcg
     7501 gtgtggcctg ggaccatatc aatgtgtttc cgactttcga aatttatgtg gatcagttcc
     7561 ggaaatttat tgagctgata gaacccgacg gaagcatagt gtattgcgaa aacgatgaag
     7621 aactcaaatg catttgcccc gaagcccgaa aggacataaa attactgcca tacggtattc
     7681 cgcagcattc cataaataac ggaacaacct ctgttgctat cggcgataaa gctacagata
     7741 tccgggtttt tgggaaacac aacctgatga accttaacgg tgccaggctg gtatgcgagt
     7801 gcctgggtat ttctaccacg gattttatga gggcgatcgc cggatttaag ggggcggcaa
     7861 agcggcttga actgcttggt aaaaatgagg attccgcttt ttacaaagat tttgcacatt
     7921 cgccttccaa gctgagagcg accattgatg cggtgaagga acagtttccg caacgggagc
     7981 ttgttgcctg tatggagctg catacataca gcagtctgaa cagcgatttt ctggaacaat
     8041 ataagggatg catgcaaaaa gccgataaag ccatcgtttt ttacagcgcc catgccctcg
     8101 aaataaaaaa gatgccgcca ttgcccgaaa ataaggtaaa aggttcattt ggccgcgatg
     8161 atattacggt ctttacaaat aaagaaaaac ttgttgaata tcttgaaagc ctcgactgga
     8221 aaggcaaaaa cctgctgatg atgagttctg ggagttttga cggactggat ttacttgagt
     8281 ttgttaaaaa tcttgagatt tgacattagt acccaatcta aaaattaatt taattgataa
     8341 actgaaggaa atcaattaaa ttccgtcaaa caactgacgg aaatcgaaaa agatgagttc
     8401 tacaggaatg gctcaatcgg aaagtatgct acaatgaaat tttagtttat gtatggttta
     8461 gctgcttcca tacagtagtt ccagactgtc ttttcatttt caatggagta atatttttcg
     8521 cttggttttt cttcaccttt aggcccgaaa tacctgcctg ttactccttc cttttctggg
     8581 gcggtggccg ttttgatgct gctgcttgct ccttttgcta cagaattcat cattggtaac
     8641 caaagaatac ctattatttt ccactttaat gacttcatgg attcccttcc caggcttgta
     8701 attgctgagc ccggatgtac ggtgttgaac gtgatgtttt cgatgccggc acttatcatt
     8761 tcagcaacaa aatgccgcat aatccaaatc acgtataact tggataaacc gtaagctttt
     8821 ggcatagtat aattgttctt taactctatg tcgtcaaggt acggcttgcc tcccatctta
     8881 tgtgcagccg acgatacggt aactacacgt gccgacttgc tttttttaag catatccagc
     8941 aaaaggtgtg tcaggagaaa gggcgccaag agattgatgg tcatggtttt ttcatgacct
     9001 tcaggcgttg tctcccgttt tgctgtgtat tgtgcaccag cattattgat cagcacgtct
     9061 atatgatcgt acttttcttt tattgcatca gcaaaacgtt ttacttcggc aagcgaaaga
     9121 aaatcggcag tgaacatatc aatattgctg ttgcccgatt ctgcctttat ttcgtcataa
     9181 acagcctgta atttttgctt gttacgaccg tgaataatta atgtgtgccc ttgttttgca
     9241 agggttttgg cagtttcttt gccgataccg tctgatgcgc cagtgattac tattattttg
     9301 ctttgcataa aaatatgtgt ttgaattttt actgattcat ttaattgttc ttttagcaag
     9361 gtcttttata tccttcacat aaatcacgtt ttgtttgtag ctgttttgag ccacgtaaat
     9421 catagattgt gaaacctgtt tcatcgtgct gtacataaat ggaaataaga ttctgattac
     9481 aggaaacagc cacgagatat aaggatagta ttttaaaacg tgcacttgtc cagccacagg
     9541 tttaacaaag ccaatacggt agccgaacgc ctgtttaaac ggcaatttca tcagatcgtt
     9601 ttcggttttc ccttttactc ttgcccacat catgcggcct ttttcggtgc tgtcggttcc
     9661 tccgccactt acataaataa aagacatggc agggttggga tttaatgctt ttgcaaaatg
     9721 aagcgtggta tcataggtaa tttttgtata ttcttcttct tttttcccta cggaacttat
     9781 acctgcacag aaaaaacagg catcataacc ttgtagtttt tcatcatctt cttttagcgt
     9841 taaaaaatcc ggcacgatgt attcttttag ttttggatgc gattttcccg ttggtttccg
     9901 gcttacactc agcacttcgg taattgaagg atgtgtcaga ctttcgatta aaactccttc
     9961 gcctatcatt cctgttgttc ctgttattat gactttcatg tttctgtgtt tttgtgaaaa
    10021 tttatttgcc catgcaaaag taaatgttac ttttgtgacc aacaagtata gagtatggtc
    10081 tatacttgaa ataaatttca ataaactgaa aagtgtatgt taatcaatga attgtcgaag
    10141 aaaacgggcg tgtcgataca cacgttgagg tattatgaaa acctgggttt gattcaaggt
    10201 gtttcggacg aaaaggtaac aaccaacaac tacaaaaatt atgatgaaat tcaggttgaa
    10261 cgggttgaaa tcattaagga agcaaaagag gttggcttta cactatctga aataaagaaa
    10321 atgctggata gctggtatag cggattattg actccccggg agcaggtgaa aatgtttgaa
    10381 gcaaaagtca acgatatcga aattaagatc cgtcatttaa aacaggtcaa aaaaagactt
    10441 gaaaaaatga ttagggaagt tgaaaagggc gtgaattgcc agccgaaata accttaggcg
    10501 aagactgatc ttagtgactg ataaaaggaa atacaaagaa aataatcaaa acgattttct
    10561 catgttctac tattatttaa taatcattgg taacgcttat gtgcgtgagg tgctccataa
    10621 aaatagttcc ataagacaat atcggaattg tactatccgc tgcaacggat tgaatgtgcc
    10681 ctaagtatta caacctaaac ttttgtatct ttgcaatgta aacttttgta tctttgcaat
    10741 gtaaactatt atatttttgt attataaact aataatccga attgtatggt ttttaaatct
    10801 gaaatagaga gcgcttataa tctccagcag tccgatgtta aaataaaaaa atctggcgta
    10861 aagcgtgcct atctgagcaa actattgcct gaaagcaaac atattgaagt gatttctggc
    10921 atccgtagat gcggtaaaag caccttgatg aaacaattga tgcagcaata taagacagat
    10981 attgcctatt ttaattttga agacccgcgt gtaaataatt ttgagatagg agattttcaa
    11041 aaattagatg aaattatagg aagaaataaa gctgcatatt tttttgatga aattcaggat
    11101 gttgcttcgt gggaaatttt tgtcaggcaa ttgcatgaca gaggcgaaaa aatctatatt
    11161 acgggttcca atgcttcact gttaagcaag gagttgggca cgcgccttac gggaaggcat
    11221 cttcgccacg aattgtttcc tttttcatat cttgaatttt tggagtataa aaacctcaaa
    11281 aacaatgaaa actcttttga aaaatacatc caaaaaggcg gtttccctga atttttaaga
    11341 gaagagaacc aggaaatatt acaatcactg ctgaaagata ttattttgcg agatattgcc
    11401 gtacgttacg gaattaaaaa ctccaaaaca ctgatggata ttacactctt tctgatatcc
    11461 aacattggga aagaaactac ctataacagt ttgcgaaaaa gcttttctgt tggctctgca
    11521 aatacagtgt ccgattatct ttcgtggctg gaagatactt atattttgtt ttttttaccc
    11581 agattcagtt ggtcggcaaa aagtattgct gttaatccaa gaaaagttta tgccatcgat
    11641 aatggacttg caattgctaa ttctctgagt tttacgaacg ataaaggcag actacttgaa
    11701 aatgctgttt acctgttttt aaggcaaaaa tttaattcac tatattattt cagggaaaac
    11761 agcgagtgtg attttgttgt cttcggaaag ggcaaatgcc ttttgcttat tcaggtttgc
    11821 gagcaggttc ataacgaaaa taaagacaga gaagttaatg gtctcctgga tgcgatgcgt
    11881 ttttttaa
//
LOCUS       DAOSFP010000014        11707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4633_length_11707_cov_4.317800, whole genome shotgun sequence.
ACCESSION   DAOSFP010000014 DAOSFP010000000
VERSION     DAOSFP010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4633_length_11707_cov_4.317800"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLI16_00905"
     CDS             <1..573
                     /locus_tag="PLI16_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83148.1"
                     /translation="PVLTTGTISPATYVVDAANGSSISVPFTLTGTMNAGNIVTAQLS
                     DDAGLFASPVTLGTLATTTSGTISGTIPAGTPTGGGYRIRVVSSDYALVAADNGSDLV
                     ITNTTGISDISAGSYSIYFSENELISDLKLTQTIHPQIQLFNLSGQFVAGFELQNNAV
                     NHYEMNIPAGIYMYRLINGDQMISGKIVKF"
     gene            complement(635..1117)
                     /locus_tag="PLI16_00910"
     CDS             complement(635..1117)
                     /locus_tag="PLI16_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOH83149.1"
                     /translation="MKTFLDLSATGIDGKEISMSTYKGKKILVVNTASKCGFTPQYKD
                     LERLYREFGPEKFVILGFPANNFLWQEPKTNDEIASFCSLNYDVTFPMFAKISVKGSK
                     QHPVYEWLTKKELNGKADARVKWNFQKFMISENGEWLDMVPPAESPYCEKIVNWLRKG
                     "
     gene            complement(1269..1691)
                     /locus_tag="PLI16_00915"
     CDS             complement(1269..1691)
                     /locus_tag="PLI16_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83150.1"
                     /translation="MKKQILFIICFLFFLNVFSQEKERTNDVNRHFDAAMFPKGITTI
                     NVYSSKECGRCGNVVELLRKDSIVFNEFDLTDPKVFDEMNAKVYKAVPYKDLTVTTEY
                     PVFEIDSIIFHHIEDHVKFANDLIEYLHGKKEDKVNPE"
     gene            complement(1744..2739)
                     /locus_tag="PLI16_00920"
     CDS             complement(1744..2739)
                     /locus_tag="PLI16_00920"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOH83151.1"
                     /translation="MIQKIRAAITGVSGYLPDDILDNATLEKIVDTTDEWIRSRTGIL
                     ERRILREPGKGTSDLGAGAVKLLLEKTGNKAEDIDLLVCATVTPDMQFPATANIICDK
                     TGIKNAFSFDLNAGCSGFVYALVTASKFVETGQCKKVIVVGCEKMSSIVDYTDRATCI
                     IFGDGAGAVLLEPTIENTGIIDSLMSADGSGRIYLHQKAGGSLKPASHKTIDDREHFI
                     YQEGQPVFKVAVVKMAEATEQIMKRNGLTAETLSWLVPHQANLRIIEATARRIGIDKE
                     KTMINIEKYGNTTAATIPLCLWEWERKLKKGDNLVLVAFGAGFTWGSVYLKWAYN"
     gene            complement(2831..3013)
                     /gene="rpmF"
                     /locus_tag="PLI16_00925"
                     /pseudo
     CDS             complement(2831..3013)
                     /gene="rpmF"
                     /locus_tag="PLI16_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
     gene            complement(3018..3542)
                     /locus_tag="PLI16_00930"
     CDS             complement(3018..3542)
                     /locus_tag="PLI16_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOH83152.1"
                     /translation="MSYQDRYILEFAGLSDGMHHFEFTVDGNFFREYDYEDIQQCNIL
                     VLLDMNKQPDMLELKMNFSGTIVCECDRCLEKYDQPVAGNERLIVKFGDEDTEPADDI
                     IMLPPQENKVDLSQFFYETIILLLPQRRVHPEDENGKSNCNPDIIKKLEELNQQKKNN
                     DPRWDELKKITFKN"
     gene            complement(3633..6866)
                     /locus_tag="PLI16_00935"
     CDS             complement(3633..6866)
                     /locus_tag="PLI16_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH83153.1"
                     /translation="MLKITAHPILEVKDTPEVTFLYEGKTLKGQSGFTIAAALHQAGY
                     PVHSHSLKNRERSLECGIGKCGACEMLVDGQIKRICITLVDGVKEVREIPKDYAPEMN
                     PPETQKPHDVYKTDVVIIGAGPAGLACREQLNKHAVSNIVLDNNDKIGGQFIMQTHQF
                     FFFEKEKKFGGMRGFDIATTLAGDKHDGIMLNATVWDILEGNRIAAKNLKTGDIFFVD
                     AEYMVVATGAVPFMPAFENDDLPGVYTAAVVQKMMNNEFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGAHVKAIIEAMPREGGFPVQANRVRRLGIPVMLSHILIKALPNENHDGIVG
                     AVVAECENFNPIPGTEKTIYGIDAINICTGLVPDDQLLIKGNEIFGRNCFGAGDAIRI
                     GEGTSAVVRGKQVAFEILNEMEEHFDYDEFLATSKEYIDSQQHPIRIIQEPYKPTSQR
                     MEARPFVQIDCLYGFACNPCEFACPHGAITKTSTSTVPQIDFDKCTGCMECVYQCPGL
                     AIFGYNIKKDWLFLPVEYYVEEGAAVFLVDNNGEKLGEGVIEKVLKKPNKTNVVRAKS
                     LTLHGEDLIAVRGFIIKENYPEKITFEKAEKAKETEKYICHCDDVTLTEILNVIGDRR
                     FISVDEIKHTTRLGMGACRGKRCIRRLKQQLKSYDISVVGEATPRGPLSNQINFGELY
                     PKSVPEKVHVSNQGIAPEIVKVASFVAGGGIGGSALFRYLAEAGMKPVLANFGRGSSW
                     RNIAGGRPGFSLPEMADLAVHNLEIFKELQKFKNIDYHRTRYVTFAHDETMYKALVDS
                     LSWSDAVMVEPKDFARVISPYLNTNLNKYLAAQVSENCWQATPGRVIDLIRRIGINNG
                     GTVLEDCKMISLRKEENTFKILVRNHQEKYVEYHTEVFINALGFEGEKFARMLGLETG
                     LYPVKHQAFITRRLPMMGLNGNPLDMLIDRRRYKGFVAVYGQQLAETGQIIGCASPAV
                     DPQQADKNLKINSQDFLEIATEIFIDWIPQLSSVGFQAVWAGYYVEPRMIIDTEHGLF
                     LGLRGQGFMLGQYLAKLYVDALTGKKVPEYFSRLNLNGDGLPEKAFK"
     gene            6992..7348
                     /locus_tag="PLI16_00940"
     CDS             6992..7348
                     /locus_tag="PLI16_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOH83154.1"
                     /translation="MSAKLKDFNDYRSKMNEKLLASDNLVLKRFFNLDTNAYLDGALP
                     TITKEMLGLVSSMVLRCDDCIRYHLIKCHEHKLTRAQLMEIFSIANIVGGSIVIPHTR
                     RALEFWEELEKEEKNI"
     gene            complement(7650..8306)
                     /locus_tag="PLI16_00945"
     CDS             complement(7650..8306)
                     /locus_tag="PLI16_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83155.1"
                     /translation="MAKTVQPSMAKKIIFASIIFLTAICSCHNKKPVPAENNSSPAPT
                     QKEIAVNKDSLLLELTDQILAALEAKDYPKISSYFHPVYGVRFSPYAYIDTLADVRLK
                     KETFNELVKKQTKLRWGSFNGSGEDIFMTVPQYFGRFVYNANYKKDGEKYVNKVNGKG
                     NTKNNMNVIYQGLDFIECYIPGFDKKMDGMDWCSLRLVFKKYHEQYFLVGIVHDQWTI
                     "
     gene            complement(8530..11370)
                     /locus_tag="PLI16_00950"
     CDS             complement(8530..11370)
                     /locus_tag="PLI16_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOH83156.1"
                     /translation="MKKHTVLLIFFYFLFSSPVFSQILLNGTIESFDEMCKIETHPTI
                     MDDGTILLMDFYIPVTQDCVISIINVPGTGNVPIQIIPRNTQYIIYDSINNQLNPNPY
                     QLPLVFTRTPYNKNGDQSGPIFPFMGYIFGAQDMRGAHSSSGVYFPMYSDSWNKNFYH
                     PDIHYNIDITDPNDPQNANKHEDGYQTLQRLMNDIKRVYDYNGDQQPDTFLISNGSIG
                     MMGGSALGNSQYTLAATSPINSSQPGLQSLLPVIASADHYLYTLTNNGVYRQALVDNW
                     ISGQINSLVDDSLNATDNSILNNLHSSTDYGQTNKAIVASKAIDFLLAEGPIPGYYPD
                     SPLRASMDASSAPVDASGNPDSNGTYSRFRNMDVPAYHVSGWWDIFVDGQIYTFNKMR
                     DEALSVETNKLQKLVIGPWAHQTVGGLTTGDMTYPQNVSDVLGFDISNLSFSMSNSGF
                     IVDLLESEIMNWFKYTLNKKGVPSTSQAKFIIPESHVWQNIGVDLDVRVPSEDYIVPH
                     ITFIKYLAGQATLDNLKIEIKTVTDTIPYTYTIPVLNPPMLNVTDTIHSSSVIDFDTV
                     PAIRLYLVGPVNDGVPENDNLGNYWIKCDSFPFNDGIHYTRFYLHGDSTLNTQMPTTN
                     EGCLTYIHDPDHPVLTVGGVNMTVQTPAGPPRKSQGQMNLADPLFVNYTMNNPGVIQF
                     TSGLVEDSLRILGFPTATLYAQSDVSGGGPTDADFFVRILDVYPDGRELFVVEGCVNA
                     RAREYVRSLYNNNENIHAPYTNITSGTVYEYQLKMMPVGYVWGKNHKVKVLISSGNYP
                     RYQSNPNLPMMNYDFFRRKPNDGQQYNYSGTLMSPRIAQQSIHFSPAYPSHIALPVFD
                     NLPSNFIADQKPSKEYSWSVYPNPCSDYAFVNLPNEKGLVQVYSTNGQLLEEKETDKS
                     FVLDVSRYAPGIYVVKFRSENLTLSRKIIVNR"
BASE COUNT         3072 a         2494 c         2596 g         3545 t
ORIGIN      
        1 ccagtattaa ccaccggaac catctcgcct gcaacttatg ttgttgatgc agctaatggc
       61 tcatccatca gtgttccatt tactcttacc ggaacaatga atgcagggaa tattgtaacg
      121 gcacaacttt cggatgatgc cggtttgttt gcatcgcctg ttacgctcgg aacactggca
      181 accacaacct ccggaactat atcgggtacc atcccggcag gcacaccgac aggcggcgga
      241 tatcgcatac gcgtggtttc ttctgattat gctcttgtcg cagcagacaa tggttctgac
      301 ctcgtgataa caaatactac agggatcagc gatatttcag ccggcagcta cagcatatat
      361 ttttcggaaa atgaactgat aagcgacctg aaacttacac aaactattca tccgcaaatt
      421 caactgttta acctgagcgg acaattcgtt gccggttttg aattacaaaa caatgcggtt
      481 aatcattacg agatgaatat tcctgcaggt atttatatgt atcgcctgat taatggtgat
      541 caaatgatct ccggaaaaat tgtaaagttt taatttaagc ttactttacg atttacgggg
      601 ctgcatttca acgaaatgca gccccgtaat gttttcagcc ttttcgcagc cagtttacta
      661 ttttttcgca ataagggctt tcggcgggag gcaccatgtc aagccattcg ccattttcgc
      721 tgatcataaa tttctggaaa ttccatttca ctctggcatc ggcctttcca ttgagttctt
      781 ttttggtaag ccattcataa acaggatgtt gttttgatcc ttttacagaa attttggcaa
      841 acatcgggaa ggtaacatcg taattgagag aacaaaaact ggctatttcg tcattggttt
      901 tgggttcctg ccacaaaaaa ttattggccg gaaatccaag aatcacaaat ttttctggac
      961 caaactcccg gtaaagcctt tcgagatctt tatattgcgg agtaaatccg catttggagg
     1021 ccgtgttcac cacaagtatc ttctttcctt tatacgttga catacttatc tctttgccgt
     1081 caattccggt ggcacttaaa tccagaaatg ttttcatgat actccttatt ttaaaatgca
     1141 gataaaggta atgaatatcc gtatcaaatc cataaaaaat gacccgggag tgatgtgttt
     1201 gttgcctttt tgataaaccc tggcagcaag ccctgtaatg tggatgtgtt gttgcgaaaa
     1261 ggaatttttt attcgggatt taccttatcc tcttttttgc cgtggaggta ttcaataaga
     1321 tcgttagcaa atttcacatg atcttcgatg tgatgaaaga taatagaatc tatttcaaaa
     1381 accgggtact cggttgtgac agtaaggtct ttgtaaggca ctgctttata tactttagca
     1441 ttcatttcat caaaaacttt ggggtcggtg aggtcaaact cattaaaaac aatactgtct
     1501 tttctgagca attcaaccac attgccgcat cggccgcatt ccttgctgga ataaacatta
     1561 atggtggtga tgccctttgg aaacatggca gcgtcaaaat gcctgttcac atcgttggtt
     1621 ctttcttttt cctgagaaaa aacattcaga aaaaacaaaa aacaaataat aaataaaatc
     1681 tgctttttca tctttctgta tttttttaag acacaaaatt tttcacctaa ttctaaaaca
     1741 caatcagtta tacgcccatt tcaggtacac gcttccccag gtaaatcctg ctccgaaagc
     1801 aaccaaaaca aggttgtcgc cttttttcag tttgcgttcc cattcccaaa ggcacagcgg
     1861 aatagttgcc gcagtggtat tgccatattt ttcaatgttg atcatggttt tttccttgtc
     1921 gataccaata cggcgggcgg tggcctcgat gatgcggagg ttggcctggt ggggtaccag
     1981 ccaggagagt gtttcggcgg tgaggccgtt tcgtttcatg atttgttcgg tggcttcggc
     2041 catttttact acagccactt taaaaaccgg ctgtccttcc tggtagataa aatgttccct
     2101 gtcgtcaatt gttttatggc tggctggttt caaggaaccg ccggcctttt ggtgcaagta
     2161 aatccttccg ctaccatccg cactcatcag tgaatcaatg atgccggtat tttcgatggt
     2221 cggttcaaga agaacggctc cggcgccatc gccaaaaatt atgcaggtag cgcggtcggt
     2281 gtagtccact atagaagaca ttttttcgca acccaccacg attacttttt tacattgccc
     2341 ggtttccaca aactttgatg ccgttaccag tgcataaaca aagccggaac aacctgcatt
     2401 caggtcgaag ctaaaagcat ttttaatgcc ggttttgtcg cagataatat ttgcggtggc
     2461 gggaaactgc atatcgggag tcacggtggc gcacaccagc agatcgatgt cttccgcttt
     2521 gtttccggtt ttttccaata ataatttcac ggctccggca cccagatcag atgtgccttt
     2581 tccgggttct ctcaggattc tcctttcgag gataccggta cgggaacgta tccattcgtc
     2641 ggtggtatcc acaatttttt caagtgtggc attgtcaagg atatcgtcgg gcagataccc
     2701 gctaacacca gtaattgccg ccctgatttt ttgaatcata agaatcaatt aatagttacg
     2761 ggagctattt cttttttcct tttgggggtt cttttttggt aacttttttg ggttcttttt
     2821 ttgtttctac ctgtgcttcg gtcttatcaa tggcaggttt tcctttataa tatccgcatt
     2881 cagggcatac cctgtgatac agcacgggtg ctccacagtt ggaacatact gaaatagtgg
     2941 ggtctgtagc tttgtcgtgg gttcttcttt ttaaggttct ggtttttgaa aaccttcgtg
     3001 ttggatgtgg catggcatta atttttaaac gttattttct ttaactcatc ccagcggggg
     3061 tcgttatttt ttttttgttg gttaagttct tctaattttt taattatgtc aggattacag
     3121 ttgcttttac cattttcgtc ttcaggatgc acccttcgtt gggggagcaa caagataatg
     3181 gtttcgtaaa agaactggct caggtccact ttgttttcct gcggcggcaa cataatgatg
     3241 tcgtcagcag gttcagtgtc ttcatcgcca aatttcacta tcagccgttc gttgccggct
     3301 accggctggt catatttttc caggcagcgg tcgcattcgc agacaatggt tccgctgaag
     3361 ttcattttga gctccagcat atcgggctgt ttgttcatgt cgagcagtac aaggatattg
     3421 cattgttgaa tatcttcgta gtcatactcg cggaagaagt tgccgtccac ggtgaattca
     3481 aaatgatgca taccatcact caatcctgca aactccaata tgtaacggtc ctgataactc
     3541 actatctttt aaataaaagt ttgcaaagtt acgaaatttt tcttttgcaa aaacaatttc
     3601 gacaaaaaag ccgttaaatc aacagtttac cgttatttga atgctttttc gggtagaccg
     3661 tcaccgttta aattcaggcg gctgaaatat tccggcactt ttttccctgt aagggcatcc
     3721 acataaagtt tagccaggta ttgtcccagc ataaagcctt gtccccgcaa accgaggaag
     3781 agtccgtgtt cggtatcgat tatcatacgg ggctccacat aatatccggc ccatacagcc
     3841 tgaaaaccaa cagaagacag ttgaggtatc cagtcgatga atatttcggt agctatttca
     3901 aggaaatcct gtgagttgat tttcaggttt ttgtcagcct gttgtggatc aacagccgga
     3961 gaagcgcatc caattatctg tcctgtttct gccagctgtt gcccgtacac ggcaacaaaa
     4021 cctttgtagc ggcgacggtc gatcagcatg tccagggggt tgccattcaa tcccatcatg
     4081 ggaagccttc tggtgataaa agcctggtgt ttaacaggat acaaaccggt ttctaatccc
     4141 agcatgcggg caaacttttc gccttcgaag cccaaagcgt tgataaacac ctctgtatgg
     4201 tattccacat atttttcttg gtggttgcgg acaagaattt taaaggtgtt ttcctctttt
     4261 ctgaggctga tcattttgca atcttcaagt acggttccgc cattattgat acctatgcgc
     4321 cgtatcaggt cgataacgcg tccgggggtg gcctgccagc aattttctga gacctgtgcg
     4381 gcaagatatt tattcaggtt ggtattgagg tagggcgaaa ttaccctggc aaaatctttt
     4441 ggctccacca taacggcatc ggaccacgac agggaatcga ccagcgcttt atacatggtt
     4501 tcgtcgtggg caaaagttac ataacgtgtc cggtggtagt caatattttt aaacttttgt
     4561 aattctttga atatctcgag attatgtacg gcgagatcgg ccatctcggg caaactgaag
     4621 ccgggccgtc cgccggcgat gttgcgccag gaagaacccc gtccgaagtt agccaatacc
     4681 ggtttcatgc cggcctcagc cagatagcgg aacaaagcgc tgcctccgat acccccgccg
     4741 gctacaaaag aagcaacctt gacaatttca ggagcaatac cctggttgct gacatgtact
     4801 ttttcaggaa ctgatttagg atatagctcg ccgaagttaa tctggttcga caacggcccg
     4861 cgcggtgtag cctcgccaac aacagagata tcataacttt ttaattgttg tttcaaccgg
     4921 cggatacatc gtttgccgcg gcaggcgccc atgcccagac gggtagtgtg tttaatttcg
     4981 tccaccgaaa taaacctgcg gtcaccgatc acattaagaa tctctgtcag ggtaacatcg
     5041 tcgcaatgac aaatatattt ctctgtttct tttgcttttt cggctttttc aaatgttatc
     5101 ttttccggat agttttcttt tataataaat ccacggacag caataaggtc ttcaccatgg
     5161 agggtcagtg atttagccct gaccacattt gttttattag gtttttttaa tactttttct
     5221 ataacacctt ctcccagttt ttcaccgttg ttgtcaacca aaaaaacggc ggcgccttct
     5281 tccacgtaat attccacggg cagaaacagc cagtcttttt taatgttgta tccaaagatg
     5341 gctaatcccg ggcattggta aacacactcc atgcatccgg tgcatttgtc gaaatcaatc
     5401 tgtggcacgg tgctggttga tgttttggtg atggctccgt gcgggcaggc gaattcgcag
     5461 gggttgcagg caaagccgta gaggcagtcg atttgcacaa aaggacgggc ttccatgcgc
     5521 tgactggttg gtttgtaggg ctcctgaata attcttatgg gatgttgctg cgaatcgatg
     5581 tattctttgg atgtggcaag aaattcgtca tagtcgaagt gttcttccat ctcgttgagt
     5641 atttcaaagg ctacctgttt acctctaacc acagcgctgg tgccttcacc gatacgtatg
     5701 gcatctccgg ccccgaaaca attccgtcca aagatttcgt ttccttttat taatagttgg
     5761 tcgtcgggaa ccaggccggt acagatatta atggcatcaa ttccataaat ggttttttcg
     5821 gttcccggga tggggttaaa gttttcgcat tcggccacca cggcacccac aatgccgtcg
     5881 tggttttcgt tgggcagggc ttttataagg atgtgcgaca gcatgacggg gatacccagt
     5941 ctcctcacgc ggttggcctg tacggggaac cctccttcgc ggggcatggc ctcgatgata
     6001 gctttcacat gagcgccggc ctgcatcaac tgataagaag taaggtaacc gatgtttccg
     6061 gctcctacgg tcaacacatt tttgccgagc agggtaaact cgttgttcat cattttttgg
     6121 acaacggcag cggtgtatac acccggcagg tcgtcgtttt caaaagccgg cataaaaggt
     6181 acggcgcctg tggctaccac catatattcg gcatcaacaa aaaagatgtc gccggttttc
     6241 aggtttttgg cggcaatacg gtttccttct aggatatccc acacggtggc gttgagcatg
     6301 atgccgtcgt gtttgtcgcc ggctagggtg gtggcaatat caaatccgcg cattccgccg
     6361 aatttttttt ctttttcaaa aaagaaaaac tgatgggttt gcatgataaa ctgcccgcca
     6421 atcttgtcgt tgttgtcgag gacgatattg gaaacggcat gtttgttaag ttgttcgcgg
     6481 caggctagtc cggcggggcc ggcgccaatg atgaccacat cggtcttata aacatcgtgc
     6541 ggcttttgtg tttccggcgg gttcatctcg ggggcataat ctttgggaat ctctcgtact
     6601 tcttttacgc catcaaccag tgttatgcat attcttttta tttgtccgtc gaccagcatt
     6661 tcgcaggccc cgcattttcc aatgccgcac tcaagactgc gttcgcggtt tttgaggctg
     6721 tggctgtgca caggatatcc cgcctggtgc agggcggcag caatagtaaa tccggattgt
     6781 cctttaaggg ttttgccttc gtataggaat gtaacttcgg gagtgtcttt aacttcaaga
     6841 atcgggtgtg cagttatttt taacatggag ttgaattatt tggttgagaa aaatactatt
     6901 tttgaaaaaa acatgacaaa tttatgtttt tcggctgtag cgataatttt ttactgaaaa
     6961 tatttttccg gtaaccacaa aaaaagtgtc tatgagtgcc aaactgaaag attttaatga
     7021 ttataggagc aaaatgaatg aaaaattgct cgcgtcggat aacctggtgt taaaaagatt
     7081 ttttaatctc gacaccaatg cttacctcga tggcgcctta cctaccataa ccaaagaaat
     7141 gctgggcctg gtatcttcca tggtgcttcg ttgcgacgat tgcatcagat accacctgat
     7201 aaaatgtcat gaacacaaac tgacacgtgc acaattgatg gagatttttt ccatagccaa
     7261 tattgttggc ggctctattg tgataccgca tacgcggagg gctttggagt tttgggagga
     7321 gctggaaaaa gaagaaaaaa atatctagcc tgaaaaattt acatgaattt tttacccttc
     7381 gggctaacaa aaggaactgt attaatacag caatcaattt gaaaaacaat taaatgacgg
     7441 tggcttcggg ggcctcagcc accgggagac aatcaaatgg cggtggtttt gaggacattt
     7501 cggcaggctc aatgtaccat ctcagccacc gggagacaat caaatgacgg tggcttcgag
     7561 ggcatctcgg caggctcaat gtatcacctc agccaccgtg aaagaaaatc atgataaaat
     7621 tcactttgtt tatttgtgga caggtcaggc taaatggtcc actggtcgtg gacaatgccg
     7681 accaggaaat attgttcatg gtatttttta aatactaacc gcaaactgca ccaatccatg
     7741 ccatccattt ttttatcaaa gcccggaatg taacattcaa taaaatcaag gccctggtag
     7801 atgacattca tattgttttt tgtgtttcct ttgccgttta ccttgttaac atatttttca
     7861 ccgtctttct tataattggc attgtaaaca aagcggccga agtattgcgg gaccgtcata
     7921 aaaatatctt ctccactgcc attaaagctg ccccacctca gtttggtctg tttttttacc
     7981 agttcgttaa aggtttcttt tttcagacgc acatcggcta aggtatcaat ataggcgtaa
     8041 ggtgaaaacc ggactccgta aaccggatga aaatatgagg atattttggg gtagtccttc
     8101 gcctccaggg ccgctaagat ctggtcggtc aattcaagca acagcgaatc tttgtttaca
     8161 gcaatctctt tttgagttgg tgcgggggat gaattatttt cggcgggaac aggctttttg
     8221 ttgtgacatg aacagatggc cgtgagaaat atgattgaag cgaatatgat tttttttgcc
     8281 atgcttggtt gaacagtttt tgccattttt tttatttttt aaacaaaaat aatgataatc
     8341 acagattgcg caataggaac gtagtgactc ctgccaatca ttgactgggg cggctgatta
     8401 taacgattgc ccatatgtaa tagtccaacg aattggccta taacatctgt tacatcggcc
     8461 tgtctgactg gccatccagg caggcattat accataaatc ccgtacatat tttaaatttc
     8521 taattggtat tatctgttca caataatttt ccggctgaga gtgagatttt cggaacggaa
     8581 ttttacgaca tatattccgg gagcataccg gctgacgtcc agaacgaaag atttatccgt
     8641 ctctttctct tctaataatt ggccattagt tgaataaacc tgcaccaggc ctttttcgtt
     8701 tggcaaatta acaaatgcgt agtcagaaca gggattagga taaacactcc aggaatattc
     8761 ctttgaaggc ttttgatcag caataaaatt ggaaggaaga ttgtcaaaaa ccggcagggc
     8821 gatatgactg ggataagccg gggaaaaatg tatgctttgc tgtgcaattc tggggctcat
     8881 cagggttccg gaataattat attgctgacc atcattgggt ttgcgtctga aaaaatcata
     8941 attcatcatc ggcagattag ggttagattg gtaccgcgga taatttccgc tgcttatgag
     9001 aaccttgacc ttatgatttt tgccccatac atatccgaca ggcatcattt ttaactgata
     9061 ttcgtaaacg gttccggagg taatattcgt ataaggagcg tggatatttt cattgttgtt
     9121 gtataaagac ctcacatatt cccttgctct tgcatttaca caaccttcca ccacaaataa
     9181 ttctcttccg tcgggataga catccagaat gcggacaaaa aagtctgcat cggtgggacc
     9241 acctcctgat acatcggatt gagcatacag ggttgccgtc gggaacccca atattcttag
     9301 cgaatcctcc acaagcccgc ttgtaaattg aattactccc ggattattca ttgtataatt
     9361 aacaaataag ggatctgcaa ggttcatctg cccctggctt tttctgggag gacccgccgg
     9421 cgtctgtacg gtcatgttta cccctccaac ggtcaacaca ggatgatcgg ggtcgtgaat
     9481 ataagtcagg caaccttcat ttgttgtggg catttgggta tttagagtac tgtcgccatg
     9541 taaataaaag cgggtataat gaatcccgtc attaaaaggg aaactgtcgc attttatcca
     9601 atagtttcca aggttgtcat tttcaggtac gccgtcattt accggtccga caaggtacag
     9661 gcgaatggca ggaaccgtat caaaatcaat tactgagctg ctatgaatgg tgtcggtaac
     9721 attaagcatc gggggattaa gcaccggaat agtataggta taaggaatgg tatcggtgac
     9781 tgttttgatt tctattttta aattgtctaa tgtggcctga ccagctaaat acttaataaa
     9841 tgtaatatga gggacaatat aatcttccga aggaaccctg acatcaagat caactcctat
     9901 gttttgccat acatggcttt caggaataat aaatttggcc tgggaagtgg aggggactcc
     9961 ttttttgttc agcgtgtact taaaccagtt cattatttca gattccaaaa ggtcaacaat
    10021 aaaccctgaa ttactcattg aaaaacttaa attggaaatg tcaaaaccta atacatctga
    10081 aacgttctgc ggatatgtca tatcgccggt agtgaggcca ccgacggtct ggtgcgccca
    10141 tgggccaata accaacttct gcaatttatt ggtttccacg ctgagggctt catccctcat
    10201 tttgttaaag gtgtatattt gcccgtcaac aaaaatatcc caccatccgc tgacgtggta
    10261 agcgggaacg tccatatttc tgaatctgct gtacgtaccg ttgctgtcgg gatttccgga
    10321 agcatctacg ggtgctgaac ttgcatccat acttgcacgt aagggactgt cgggataata
    10381 accgggtatg ggaccttcgg caagaaggaa atcgatggct ttggaagcga cgatagcttt
    10441 gtttgtctgt ccgtaatcgg ttgaactatg caggttattc aagatactgt tatctgttgc
    10501 gttgaggcta tcgtctacca atgaatttat ctggccactg atccagttgt cgaccaaagc
    10561 ctgacggtaa acaccattat ttgttaaggt atatagataa tggtcggctg atgctatgac
    10621 tggtaaaagc gactgtaaac ccggctgtga tgaatttatc ggggatgtag cagcaagggt
    10681 atattgtgaa tttcccagtg cacttccacc catcatgcct atcgagccgt tagagataag
    10741 aaaagtatcc ggctgctggt ctccgttata atcataaact cttttaatat cgttcattaa
    10801 tcgttgcaac gtttgatagc catcttcatg cttgttggca ttttgaggat cattcggatc
    10861 tgtaatgtca atattatagt gaatatcagg atgatagaaa tttttgttcc agctatcgct
    10921 gtacatagga aaataaaccc cggaggatga atgagctcct cgcatatcct gagctccaaa
    10981 aatatatccc ataaaaggaa agataggccc gctttggtcc ccgtttttat tgtatggggt
    11041 ccttgtaaaa acaagcggta actgatatgg gttcggattc aactgattgt tgatggagtc
    11101 ataaataatg tattgcgtat tacgggggat tatctgaata ggaacatttc ctgtcccagg
    11161 aacgttgata atagaaatta cacaatcctg ggttaccgga atataaaaat ccatcaatag
    11221 gatagttccg tcatccatta ttgtcggatg ggtttcaatt ttacacatct cgtcaaaact
    11281 ttcaatagtt ccgtttaata ggatctgtga aaaaaccggt gatgaaaata aaaagtaaaa
    11341 aaatataagt aatacagtgt gctttttcat gttgtctgaa ttttttcttc aaaagtagtt
    11401 attttatttc tgatcatcgc ttgttgaaaa catgcttcct tgtaggggat attattatca
    11461 gtgcgacctg gttaatggtt aatgttattt cgttccaact tcggatcctg ataaaaaaca
    11521 aaaaaaactg ttttttataa aaaaacttgt atatttgcac tcccaaaaaa gaaaaaggga
    11581 gcatagctca gctggttcag agcatcccga cggacacgtc gggagggtca cagacgagca
    11641 aataaactgg gagtttagct cagctggttc agagcacctg ccttacaagc agggggtcac
    11701 tggttcg
//
LOCUS       DAOSFP010000015        11641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4651_length_11641_cov_4.110564, whole genome shotgun sequence.
ACCESSION   DAOSFP010000015 DAOSFP010000000
VERSION     DAOSFP010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4651_length_11641_cov_4.110564"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            815..1408
                     /locus_tag="PLI16_00955"
     CDS             815..1408
                     /locus_tag="PLI16_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83157.1"
                     /translation="MMKQPGILFSFLLMLIMVSCKPSPQKAEQYYDAVKAPIDEVFEK
                     EEALIVLINNETEKLASSPFTFQTDSTAAPVAADTGRQQIEQAYNDLLSQVSISKNKL
                     SSLTGFEKSSQLKDAALALLTEYEVLCKNEYRELLQIVKIPAEDYSSEQDDRFMQLSD
                     TIDNKLKEKIAILTDEIKMFSKKYNFKIQEDTIAVAP"
     gene            complement(1514..2881)
                     /gene="radA"
                     /locus_tag="PLI16_00960"
     CDS             complement(1514..2881)
                     /gene="radA"
                     /locus_tag="PLI16_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOH83158.1"
                     /translation="MPKIQTIFFCQNCGAQSTKWIGRCPSCNEWNTYVEEVVSRETAS
                     STKKRLSVAGQAQPRLISEINIDEHKRINSGNSELNRVLGGGIVPGSLVLIGGEPGIG
                     KSTLMLQVALSIKDITVLYITGEESEQQIKMRSERISADSKNCYILNETNTQNIFQHI
                     EKINPGMVVIDSVQTLQTELIESSAGSISQIRECTSEFQKYSKLTSTPVFLIGHITKD
                     GNIAGPKILEHMVDTVLQFEGDRNNMYRILRSVKNRFGSTSELGIFEMQSAGLREVSN
                     PSEILLSQRDENHSGIAVSSTIEGLRPLLIEVQSLVSSAAYGNPQRSSTGFDLRRMNM
                     LLAVLEKRCGFRLGAKDVFLNIAGGIRVDDPAIDLAVMASVLSSNEDIAVDMKSCFAA
                     EVGLSGEIRAVNRIEQRISEAEKLGFNEIVISKYNANNLNQKNFKIKIIAINKIEHLL
                     RYLFG"
     gene            complement(2900..3316)
                     /locus_tag="PLI16_00965"
     CDS             complement(2900..3316)
                     /locus_tag="PLI16_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83159.1"
                     /translation="MKTFQRLIYLSAIIIGIVFTVSCKKDTQEYLTGKWDRVYVNDIS
                     DISDTAFYETWEFTSDGKLFIACSGYANYIDSLDYKGELSYGMDSRNKFTVTKGNFSD
                     QWEIVLRKDNKLRIVHNYGEDQDIQQGLDEREFVKL"
     gene            complement(3370..3870)
                     /gene="rfaE2"
                     /locus_tag="PLI16_00970"
     CDS             complement(3370..3870)
                     /gene="rfaE2"
                     /locus_tag="PLI16_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOH83160.1"
                     /translation="MTTLEKIKQKIFFSAAAFEKQLEFYRFKKLKTVFTNGCFDIVHL
                     GHIDYLSKAADLGDILIVGLNSDMSVKKIKGPDRPIIDISSRSALLASLSFVDAVVIF
                     DEETPLDLIRFILPDILVKGNDYTVDTIVGAREVINNGGEVVTIDLVKGYSTTLIEKK
                     IVKNKE"
     gene            complement(3848..4882)
                     /locus_tag="PLI16_00975"
     CDS             complement(3848..4882)
                     /locus_tag="PLI16_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOH83161.1"
                     /translation="MKKKIFSVLKILLFFGIGIFFIWLSVIKLGPEDKKQIFKSFSEA
                     NYWWLVLCFVLGLISHVLRTLRWEMLIDTVGYKPSFKNTFLSLMIGYFANLAIPRLGE
                     ITRCTMLAKYEKVPFQKSFGTVIAERGLDVVTFVVLFFINLLIQYKAIAGFVYDKIYF
                     PLSEKFQFIGKGYFLYSAVALLVVFVLGIIIFNSRLMKIKLYKKIVNVIKGFWDGLKS
                     LALVKKPFTFILYTLLIWVAYFTMTYVCFFSLASTSQTPIGAVASVFVMGTIAVMVSP
                     GGIGAYPAFIEATLTLYGVPVFTSPNPVGLSLGWLIWGAQTFIIILAGVVSLILMPVL
                     NNKNDNPGKN"
     gene            complement(4888..5238)
                     /locus_tag="PLI16_00980"
     CDS             complement(4888..5238)
                     /locus_tag="PLI16_00980"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOH83162.1"
                     /translation="MFIEVLKSKIHRVQVTHSELNYIGSITIDENLMEAANIIENERV
                     YIFDINNGERFDTYVIKGKRGSGTIGINGAAARKVMVGDLIIIVSYASMDSEEAKTFS
                     PVIIFPDSATNQLK"
     gene            complement(5251..6096)
                     /gene="panC"
                     /locus_tag="PLI16_00985"
     CDS             complement(5251..6096)
                     /gene="panC"
                     /locus_tag="PLI16_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOH83163.1"
                     /translation="MRVFSYITDLKDFLQSQKQAQRTIGFVPTMGALHAGHLSLIEKA
                     KSENDICVCSIFVNPIQFNNPEDLKKYPVQTDKDMGMLREQGCDVVFCPDNKEMYPDA
                     VKEKYDFGPLERVMEGAFRPGHFNGVAVVVKRLFDIVTPDKAYFGEKDFQQLQIIRSL
                     VKQEKLPVTIVPCPIVRDTDGLALSSRNALLPPEHRRIAPQIAALLRECALLAGELSI
                     EGLKAYARQKIAATPEFVLDYFEIAAEETLQPIETWVDAKKIRAFIAVYLGKVRLIDN
                     IEINL"
     gene            6387..7220
                     /locus_tag="PLI16_00990"
     CDS             6387..7220
                     /locus_tag="PLI16_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOH83164.1"
                     /translation="MAKYKVLFVAQEIVPYLNDSHMGQICRELPQGIQEKGREIRTFM
                     PRFGCINERRNQLHEVIRLSGMNLIIDDTDHPLIIKVASIQSARLQVYFIDNDDYFQR
                     KATFADKSGLFFSDNDERAIFYARGVIETVKKLGWSPDLIHVHGWFSSLVPFFIKTAF
                     KNNPLFSDTKIVCSLYDDDFKSNFTKESVKKLKHDGVSDKDIKQYKSLDYLGMMKGMI
                     DFSDAIIQGSEKINNEVLAYAKKSNKPMLPFQPKDKYINAYSLFYDELVDELINEDAV
                     K"
     gene            7236..8564
                     /locus_tag="PLI16_00995"
     CDS             7236..8564
                     /locus_tag="PLI16_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOH83165.1"
                     /translation="MKNLRIKTRPALFLFLSLVLLSACNKNEELGLNIQPTEDIMGAL
                     FTDTIPLIAYTVKEDSFRTDEVTYNLVGSYTDPVFGTTNAGTYSQFVLTTTAVNFGNN
                     PVCDSIVLSMAYQGFYGDTASTLTLKVYELADALYYSDNYYSNDVMGVYKNNLANVSF
                     KPNFKDSVQVGSTKYPPHLRVKLNKSLGQKFIDASGSSNLADNPSFLRFFKGLYISTN
                     QMSSGGCILYLNFLSSITKLSLYYHNDADTAIYNFVVDSYCARFNSFDHKNFTSADPL
                     LKQQLAGDTTLGDSLLYIQSMAGTKIKLKFPGILGLKKLLNDTLNGRISILKAELVVK
                     LDEAATGDFAPPEKLILSKTNTDGTMSFLTDYAYGDAYYGGSYSSTTKEYRFNIGQHI
                     QDALLRGYFADKGLNLVVSGSAVKSNRAVIRGNKRSQDNLRLEIIYTKIE"
     gene            8568..10406
                     /gene="glmS"
                     /locus_tag="PLI16_01000"
     CDS             8568..10406
                     /gene="glmS"
                     /locus_tag="PLI16_01000"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOH83166.1"
                     /translation="MCGIVAYIGPKQAYPILIKGLHRLEYRGYDSAGIAILDKEMKLF
                     KSKGKVSDLEKIIENVNLKGTIGIAHTRWATHGEPNDANAHPHASNANDLALIHNGII
                     ENYAALREELISRGYIFHSNTDTEVLIHLIDEIRKNEKVDLVKAVQLALNQVIGAYAI
                     VIISKEHPDMLIAAKKGSPLVIGIGKKETFVASDATPIIEYTKNVVYLNDEEIAIIRR
                     NEDLKITTIKNIDKTPYIQKLELNLSAIEKGGFDHFMLKEIYEQPRSVRDCMRGRLNA
                     QDGRVSLGGILDYIEKMTKANRIIIVACGTSWHAGLVGEYLFEDIARIPVEVEYASEF
                     RYRNPVIYENDVVIAISQSGETADTAAAIELAKARNATIIGICNVVGSTITRMTHAGS
                     YTHAGPEIGVASTKAFTAQVTILTLMALMIADRKGSISKSRFHQLIGELELIPDKIEQ
                     TLKVNEQIIEISKIFKDVRNFLYLGRGYSFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALIDEEMPVVVIATKQHVYDKVVSNIEEVKARKGKIIAIVSEGDVEVKKIADYTI
                     EVPECDEIFTPLLATVPLQLLSYHIAVMRGCNVDQPRNLAKSVTVE"
     gene            10417..11505
                     /locus_tag="PLI16_01005"
     CDS             10417..11505
                     /locus_tag="PLI16_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOH83167.1"
                     /translation="MKIFEVVDYFEQIIPLDLQESYDNCGLQLGDTLQELTSALITLD
                     ITEKTIAEAIVKNCNLIISHHPLIFQGIKKITGATASERIIEQALKHNICIYCSHTCL
                     DNNYSGLNMFIAEKIGLQDVKILEPTKNLLKKLVVFCPVDHAENVRNALFSAGAGHIG
                     NYSACSFNTNGAGSFKAEENARPFVGEPFQLHFEPEIRIETVFPNHLRHQVIQAMLQS
                     HPYEEVAYDIYPLDNEYPRAGSGAIGTLSKDTEVAEFMAMLKNIFKTPCIRHNQTSRN
                     TIRKVALCGGSGSFLVRTAIKDKADILITADIKYHEFFNEKIILADVGHYESEIFSTE
                     LIASIITKKFPTFAFSFSDNNTNPVNYL"
BASE COUNT         3409 a         2755 c         2415 g         3062 t
ORIGIN      
        1 agggagggga aagaaggcgg tgaaaatcac aaagtaaacc ttttgagaca gccccgtttg
       61 tctgaaatgg cctatattat gtgctgataa acactcacaa aaattcattc ccaaaaaatc
      121 tgtcaaactt ttgtaggcct ttgacggcca tttcaggcac tcctttgcat gattttccca
      181 tcctgatgag agcgggcggg agcttacgtt ttggcaaggt tttttggtaa ctttttttcg
      241 gaaaaaaagt tacataaaca tgtacttgca ctgaagaaca acccacgagc catagcagtg
      301 gaattggcat atgattgcgc gcggcaatgg gttgaagcag gtcatgctcc cactgaaaca
      361 ttattgcttt tttatggtac ttttggcttg acccccgaca atgctgtcgg ggcaggccaa
      421 aagtacccaa aaagtcaaga aaatatatgg cgaaccctgc ctgaccggca ggcaggcttc
      481 actcaggcca ctccctgaca ccgccatatt ttcgggccac cgcactgttg ctgctgcgca
      541 gcaattcggc agtttcgtgc taaaatttat tttccgcatc tacaattcca gaactgcagg
      601 atcttttcta acttttttct gaattgtaca agttgagcac cttatttatt ctgttaaagt
      661 gttatgaaaa gctacgacag ccattcctgc aggccagcag gaatgttaat aaacaataac
      721 ttgattgctc gccatacaat caggtttttt cattagcatt atcaattttt cacccgcaaa
      781 ttaaaagttt tttaatttta ccgtaaactt ttttatgatg aaacaacccg gaatattatt
      841 ttcgttcctg ttaatgctga tcatggtttc gtgcaagcct tcaccccaaa aggccgaaca
      901 gtactatgat gccgtgaaag cacctattga cgaggttttt gaaaaagaag aagcgctgat
      961 tgtgctgata aataacgaaa cagaaaaact tgccagcagc ccgtttacct ttcaaacaga
     1021 ctccacggca gcgccggttg ctgccgacac cggccggcaa caaatcgaac aggcctataa
     1081 tgacttgctg tcgcaggtca gcatttcaaa aaacaaactt tcgtcactaa ccggtttcga
     1141 aaaaagcagc caactgaaag atgccgcttt agccctgctt accgaatatg aagtgttgtg
     1201 caaaaatgaa taccgcgagc tgctgcaaat tgttaaaatc cccgcagaag attattcatc
     1261 agaacaagat gacaggttca tgcaactgtc agacactatt gacaataaat tgaaagaaaa
     1321 aatcgccatc ctgactgacg aaataaaaat gttttcaaaa aaatacaatt tcaaaattca
     1381 ggaagatacc atagctgttg ccccatagcc cacattgaat tattgcaaca ggagccgcct
     1441 attgcacaag gcctctccaa aactgcttaa ttgccatgta tcttacaatc gcgaaaacga
     1501 aaagcacctg ccatcaacca aacaggtatc ttaacaagtg ttcgattttg tttatggcaa
     1561 taatttttat tttgaaattt ttctgattca ggttattggc gttatatttt gagataacga
     1621 tttcattaaa tcccagtttt tcggcttcgg atatcctttg ttcaatgcgg ttaacggcgc
     1681 gtatttcgcc cgaaagtccc acttcagcgg caaaacagct tttcatatcc acggcaatat
     1741 cttcgttgga cgacaacacc gaggccatca cggccaggtc gatggcggga tcgtccacgc
     1801 gaatgccacc ggcgatattc agaaaaacat ccttggcgcc tagtctgaac ccgcatcttt
     1861 tttcgagcac cgcaagcaac atattcatcc tccgcaggtc gaaaccggtg gaggaacgct
     1921 gtggattgcc ataagccgca gaactgacca gcgactgcac ctcaattaat aagggcctca
     1981 gtccctcgat agtggaagaa acggctatac cgctatggtt ttcatcgcgc tgcgaaagca
     2041 gtatttccga agggttggat acttcgcgca ggccagcgct ctgcatttcg aaaatgccca
     2101 gttcgctggt ggacccgaaa cggtttttta ccgaacgcag gatgcggtac atattattac
     2161 ggtcgccttc aaactgcagt acggtgtcca ccatgtgctc gagtatcttg ggcccggcaa
     2221 tgttgccgtc tttggtgatg tgtccaataa gaaaaacggg tgtggacgtg agttttgaat
     2281 atttctgaaa ttcagaagta cattccctga tttgagaaat actgcccgcc gaagattcaa
     2341 tcaactcggt ctgcaaggtt tgcaccgaat cgataacgac catgccggga tttatttttt
     2401 caatatgctg aaagatattc tgggtattgg tttcattaag gatataacaa tttttgctgt
     2461 cggcgctgat acgttcgctg cgcattttta tctgctgttc gctttcctca ccggtgatat
     2521 acaatacggt aatgtcttta atgcttaatg ccacctggag catcagtgtt gatttgccta
     2581 tgcccggctc tccgcctatc aacacaaggg agcccggcac tattcccccg cccagcacgc
     2641 ggttcagttc gctgttgccc gagttgatgc gtttgtgctc atcaatattt atttcgctaa
     2701 tcagcctggg ctgtgcctgg cctgccacgg acagtctttt ttttgtggag gaagcagttt
     2761 ctcttgaaac aacttcttcc acgtaggtat tccactcgtt gcacgatggg caacgaccta
     2821 tccattttgt agattgcgca ccacaattct ggcaaaaaaa gatggtttgg atttttggca
     2881 ttaccagaaa aaagtattcc tataacttga caaattccct ttcgtcaagg ccttgctgga
     2941 tatcctggtc ttcaccgtaa ttgtgtacta tccgtagttt attgtcttta cgcaacacga
     3001 tttcccattg gtcggaaaag ttgcctttcg taaccgtaaa cttattcctt gaatccatcc
     3061 cataacttaa ctccccttta tagtccaggc tatcaatata attcgcatat cctgaacagg
     3121 caataaataa ttttccgtca gatgtaaatt cccaggtttc ataaaaggca gtatcactaa
     3181 tatcactaat atcattaaca tacacccggt cccatttccc ggtgagatat tcctgtgtat
     3241 cttttttaca ggaaaccgta aacacaatgc caattattat tgctgacaaa taaataagcc
     3301 tttgaaaagt tttcattgtt ttatttttaa tacgacaaag ctaattatta tttattaaaa
     3361 tgcaaaccct cattccttgt ttttcactat ttttttttcg ataagtgttg tggaatagcc
     3421 ctttaccaaa tcaatagtta caacttcgcc tccattattt attacttcac gggcgcccac
     3481 gatagtatcc acggtatagt catttccctt caccagaatg tccggaagaa taaaccgaat
     3541 aaggtctaaa ggggtttctt catcaaaaat caccacggca tcaacaaaag acaacgaggc
     3601 caacagggct gatctggagc tgatatcaat gataggccgg tcagggcctt ttattttttt
     3661 cacggacatg tcgctgttca gcccgacaat caggatatcg ccgagatcgg cagctttcga
     3721 caggtagtca atatgtccga ggtgcacgat atcaaaacag ccattggtaa aaaccgtttt
     3781 aagtttttta aaccggtaaa attccaactg tttttcgaac gctgcggcag aaaaaaaaat
     3841 cttttgttta attttttcca gggttgtcat tcttattatt taaaaccggc atcaatataa
     3901 gtgaaacgac acctgctaaa atgatgataa aggtctgtgc gccccaaatc aaccaaccca
     3961 gcgataatcc caccggattg ggcgaagtaa acacgggcac gccgtacaag gtcagcgtgg
     4021 cttcgataaa agccggatag gctccaatgc ctccgggtga aaccatcacc gctatggtgc
     4081 ccataacaaa aaccgaggct accgcgccaa tgggcgtttg gctggttgag gccaggctga
     4141 agaaacacac ataagtcata gtgaaataag ccacccaaat cagcagggta tataaaatga
     4201 atgtaaaagg tttttttacc agggccagcg atttgaggcc atcccagaaa cccttgatca
     4261 cattcacaat ttttttgtac aacttaattt tcattaacct tgagttgaaa ataatgatgc
     4321 ccagcacaaa caccaccagt aaggctaccg cgctgtaaag aaaatatcct ttacctataa
     4381 actgaaactt ttcggacagt ggaaaataga tcttatcata cacaaagccg gctattgcct
     4441 tatactgtat cagcaggtta ataaaaaaca gcaccacaaa agttaccaca tccaggcctc
     4501 tttcggcgat aacggtgcca aaggatttct ggaaaggcac tttttcatat tttgccagca
     4561 tcgtgcaccg tgttatctcg cccaggcggg gaatggcaag gttggcaaaa tatcctatca
     4621 tcagacttaa aaaagtattt ttaaacgatg gtttgtaccc aacggtatcg atgagcattt
     4681 cccaacgcag ggttcgcaac acatggctga tcaatcccag cacaaaacac aacaccagcc
     4741 accagtaatt ggcttcggaa aaagatttaa agatttgttt tttgtcttcg gggcctaatt
     4801 tgatgaccga cagccagata aaaaatatgc cgatgccaaa aaacagcaat attttcagga
     4861 cggaaaaaat tttctttttc aaaataatta cttcagttga ttggtggctg aatcgggaaa
     4921 aattataacg ggcgaaaagg ttttggcttc ttcgctgtcc atgctggcat aagaaacaat
     4981 aatgataagg tcgccaacca tgactttgcg ggctgcggcg ccgttgatgc ctatggttcc
     5041 gctgcctcgt ttgcctttga tcacataagt atcaaaacgt tcgccattgt tgatgtcaaa
     5101 aatatacacc cgttcgtttt cgatgatatt ggcagcctcc atcaggtttt catcaatggt
     5161 gatgctgcca atgtagttca actctgaatg ggttacctgt acccggtgaa tttttgattt
     5221 taatacttcg ataaacattg cgctacaaag ttacaaatta atttcgatat tgtcgattaa
     5281 acgaactttt ccgagataaa cggcaataaa ggccctgatt tttttcgcat caacccatgt
     5341 ttctataggc tgcagagttt cttcggcggc aatttcaaaa tagtcgagga caaattcggg
     5401 ggtagctgca attttttgcc gggcataagc cttgagccct tcaatgctta attcgccggc
     5461 caacagcgca cattcgcgca gcaacgcggc tatctgcggg gctatgcggc gatgttcggg
     5521 cggcaacaag gcatttcgtg agctcagcgc cagcccgtcc gtatcgcgca ctatcgggca
     5581 ggggacaatg gtgaccggca gtttttcctg tttcaccaac gacctgatga tttgtaactg
     5641 ctggaaatct ttttcgccaa aatatgcctt atcgggtgtt acaatatcga acaatcgttt
     5701 gacaacaacg gccacaccat tgaaatgtcc cgggcggaaa gccccttcca ttactctttc
     5761 gagcggaccg aaatcgtatt tttctttcac agcatcaggg tacatctcct tgttgtcggg
     5821 gcaaaacacc acatcacagc cttgttccct gagcatgccc atatccttgt ccgtctggac
     5881 aggatacttt tttaaatctt caggattgtt gaactgtatg gggttgacaa aaatgctgca
     5941 tacacaaata tcgttttcac tttttgcctt ctcaatcagc gaaaggtgtc cggcatgcag
     6001 ggcgcccatg gtagggacaa agccaatagt tcgctgcgcc tgcttttgcg attgcagaaa
     6061 atctttaagg tcggttatgt acgaaaaaac tctcatgaaa aaaatttggc aaatatactt
     6121 attccttttc aggcagtgtt taatttccgt gccgattttc aaaaaacgtg ccgctttacg
     6181 acatttacaa acacccgtca atcgcttcac ggcaggcgaa ttatatcgaa aaggtattac
     6241 gcggcattta tatagcaaaa taccctcaat tactgatagt tttatcaaaa aagaatattt
     6301 gaaaaaaact aaaaggcttg tttttgaata ataaattggt aaatttttcg tacctttgca
     6361 aactgaatac agttaaaata ttaattatgg caaaatataa ggtattgttt gttgcccagg
     6421 aaattgttcc gtacctgaac gatagtcaca tgggccagat ttgccgggag ctcccacaag
     6481 ggatacagga aaaaggaaga gaaatcagga catttatgcc tcgttttggt tgtatcaacg
     6541 aaaggcgtaa tcaactgcat gaagttatcc ggctttcagg aatgaacctt ataattgatg
     6601 ataccgacca cccgctgatt ataaaagtag cctccattca atcagcccgg ctgcaggttt
     6661 attttattga taacgacgat tattttcagc gcaaggcgac ctttgccgat aaaagcggac
     6721 tgttcttttc cgacaacgac gaacgggcaa ttttttatgc ccgcggagta atcgaaacgg
     6781 taaaaaaact tggctggtca cccgacctca tccatgtcca cggctggttt tcgagtttgg
     6841 tgcctttctt tatcaaaacg gcttttaaaa ataatccctt gttctccgat actaaaatcg
     6901 tctgttcgct ttatgatgat gacttcaaaa gcaatttcac caaagaatcc gttaaaaagc
     6961 tcaagcacga cggagtatcc gacaaagaca tcaaacaata taaaagcctg gactatctgg
     7021 ggatgatgaa gggtatgata gacttcagcg atgccatcat tcagggttcc gaaaaaataa
     7081 acaacgaagt gctggcttat gcaaaaaaat ccaataagcc catgttgcct tttcagccaa
     7141 aagacaaata catcaacgct tacagcttgt tttacgacga attggttgat gaactgatta
     7201 acgaagatgc tgtaaaataa ttaaatccta cgtatttgaa aaacctccga attaaaaccc
     7261 gtccagcctt atttctgttt ttatcgcttg tgttgctttc ggcctgcaac aagaatgaag
     7321 aattaggttt aaacatccag cccaccgaag acatcatggg agctcttttc acagacacca
     7381 tcccgttgat tgcctatacc gtaaaagaag actctttccg aaccgatgag gtaacctata
     7441 acctggtggg cagttatacc gatcctgttt tcggcactac caatgccggc acctattcgc
     7501 agtttgtgct taccaccacg gccgtgaatt tcggcaacaa cccggtatgt gactccattg
     7561 tgctgtcgat ggcttatcag ggattttacg gcgacacagc ttctaccctg accttaaaag
     7621 tatatgaact cgccgacgcc ctgtattatt ccgacaacta ttattctaac gatgtaatgg
     7681 gcgtgtataa gaataatctg gccaatgtca gttttaaacc aaactttaaa gacagcgtgc
     7741 aggtgggcag caccaaatat cctccgcatc tgcgtgtaaa gctgaataaa tccctcggac
     7801 aaaaattcat cgatgcctcc ggatcctcca acctggcaga caaccctagc tttctgaggt
     7861 ttttcaaagg actgtacatc tccaccaacc aaatgtcttc tggcggctgt attttatacc
     7921 ttaactttct gtcatccatc accaaactgt ctctctatta ccacaacgat gccgacacgg
     7981 cgatctacaa ctttgttgtt gacagctatt gcgcccggtt caacagtttc gaccacaaga
     8041 attttacctc cgccgacccc ttgctgaaac aacaattagc cggcgacacc acgctgggcg
     8101 acagtctgct gtatatacag tccatggccg gcaccaaaat aaaattaaaa tttccaggta
     8161 tccttggcct gaaaaagctt ttgaacgata cgctcaacgg caggatttcc attttaaaag
     8221 ccgagctggt ggtaaaactg gatgaggcgg ccacaggtga ctttgcacct cccgaaaaac
     8281 tcattttgtc gaaaaccaac accgacggca ccatgagttt tctgactgat tatgcatacg
     8341 gcgatgccta ttatggcggc tcctattcgt ctaccacaaa agaataccgc tttaatatcg
     8401 gtcagcacat acaagacgcc ctgctgagag gatattttgc cgacaaggga ctaaatctgg
     8461 tggtttcggg tagcgccgta aaatcaaacc gagccgtgat acgcggaaac aaaaggtcgc
     8521 aggataacct gcgcctggaa atcatttaca caaaaattga ataacccatg tgtggaatag
     8581 ttgcctatat tggccctaaa caagcatacc cgatattgat aaaagggttg caccgtctgg
     8641 aatacagggg ctacgacagt gccggaatag ccattttgga taaagaaatg aagctgttta
     8701 aaagcaaagg caaagtcagc gaccttgaaa aaatcatcga aaacgttaac ctgaaaggca
     8761 ccattggcat tgcccataca cggtgggcca cacacggcga acccaacgac gccaacgctc
     8821 acccgcatgc ctccaatgcc aacgacctgg cgctgattca caacggaatc attgaaaact
     8881 atgccgccct gcgcgaagaa cttatcagcc ggggatacat ttttcacagt aataccgata
     8941 ccgaagtgct gattcacctc attgatgaaa tcagaaaaaa cgagaaagtg gacctggtaa
     9001 aagccgtaca acttgccttg aatcaggtaa tcggggctta tgccattgtt attatttcaa
     9061 aggaacatcc cgatatgctc attgccgcaa aaaaggggag ccctctggtc atcggcatcg
     9121 gcaaaaaaga aacttttgtg gcctccgatg ccacgcccat catcgaatac accaaaaatg
     9181 tcgtgtatct aaatgatgaa gaaattgcca tcatccgccg caacgaagac ctgaaaataa
     9241 ccaccatcaa gaatatcgac aagacgcctt acatacaaaa gctcgagctt aacctgagcg
     9301 ccattgaaaa aggcggtttc gaccatttta tgctgaagga gatttatgag cagccccgtt
     9361 cggtaagaga ttgtatgcgt ggccggctca atgcccagga cggaagggtt tcgctgggcg
     9421 gcatcctgga ctatatagaa aaaatgacca aggccaaccg gattattatt gttgcctgcg
     9481 gcacctcgtg gcatgccggc ctggtgggcg aatacctgtt tgaagatatt gcccgcatac
     9541 cggtagaagt ggaatatgcc tccgaatttc gttaccgcaa cccggtaatt tatgaaaacg
     9601 acgtggttat agccatttca caatcgggcg aaaccgccga caccgctgcc gccatcgaac
     9661 tggcaaaagc acggaatgcc actatcatcg gcatctgcaa tgtggtggga tcgaccatca
     9721 cccgcatgac acacgccggc tcatacaccc acgccggccc cgaaataggc gttgcctcca
     9781 ccaaagcctt cacggcacag gtaaccatcc tgaccctgat ggcactgatg atagccgacc
     9841 gaaaaggcag catttccaaa tcgcggtttc atcagctcat cggcgaactc gaactgatac
     9901 ccgacaaaat agaacaaaca ctcaaggtaa acgagcaaat catagagatt tcaaaaatat
     9961 ttaaggatgt ccgtaatttt ctgtacctcg gcaggggata cagtttcccg gtagccctgg
    10021 aaggcgcctt aaagcttaaa gagatttctt acatacacgc cgaaggctat cccgccgctg
    10081 aaatgaaaca cggccccatt gccctcatcg acgaagaaat gccggtggtg gtgattgcca
    10141 ccaagcaaca tgtgtacgac aaggtggttt ccaatattga agaggtaaaa gcacgcaaag
    10201 ggaaaatcat tgccattgtc tctgaaggag atgtggaggt gaaaaaaatt gccgattata
    10261 ccatagaggt gcccgaatgc gatgagatat tcacgccttt gcttgccaca gtgcctctgc
    10321 aactgttgtc gtatcatatt gccgtgatgc gcggctgcaa cgtggatcag ccccgcaacc
    10381 tggccaaatc agtaacagta gaataataag gtacggatga agatttttga ggtcgttgac
    10441 tattttgaac agattatccc gcttgattta caggaatcat acgataattg cggtctgcag
    10501 ttaggcgata ccctgcagga acttaccagc gcactcatca ccctcgacat caccgaaaaa
    10561 accattgccg aggccattgt aaaaaattgc aacctgatta tctcgcacca tccgctgatt
    10621 tttcagggaa taaaaaaaat taccggcgct actgccagcg aaaggataat tgaacaggcg
    10681 ctaaaacata atatctgcat ttattgttcc catacctgcc ttgacaacaa ttattccggc
    10741 cttaatatgt tcatcgccga aaaaataggg ttgcaggatg tgaaaattct ggaacccacg
    10801 aaaaaccttc tgaaaaaatt agtggttttt tgcccggttg accatgccga aaacgtaaga
    10861 aatgcacttt tttcagccgg cgccggccat attggcaact acagcgcctg cagctttaac
    10921 accaacgggg ccggtagttt caaggccgaa gaaaatgccc ggccttttgt gggcgagcca
    10981 ttccaactgc actttgaacc cgaaatccgc atcgaaaccg tattccccaa ccacctccgc
    11041 caccaggtta tccaggccat gcttcaatca cacccttacg aagaagtggc ttatgatatc
    11101 tatcccctcg acaatgaata tcccagggcc ggatcgggcg ccatcgggac cttgtcaaaa
    11161 gacactgagg ttgccgaatt tatggctatg ttgaaaaaca ttttcaaaac cccatgcatc
    11221 cggcataacc aaacctcgcg caacaccatt cgtaaagtag cactctgcgg cggatccggc
    11281 agtttcctgg taaggacagc cataaaggac aaggccgata tcctgattac tgcagatatt
    11341 aagtaccacg agtttttcaa cgagaaaata atactggccg atgtggggca ttatgagtcg
    11401 gaaatttttt ctaccgaact aattgcctca atcataacaa aaaaatttcc tacttttgca
    11461 ttttcatttt cagataataa tacgaacccg gtaaattact tataaattaa tagtttatgg
    11521 caaagtcaac tacaaaaggt actgctgttc aggcaaaatc gaaaaaagaa accagcaacg
    11581 ctgccgtgaa aaaagaaacg gtaaatacgt ttgtgtcaga gacattgccg cttccgatat
    11641 g
//
LOCUS       DAOSFP010000016        11515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4723_length_11515_cov_4.334817, whole genome shotgun sequence.
ACCESSION   DAOSFP010000016 DAOSFP010000000
VERSION     DAOSFP010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4723_length_11515_cov_4.334817"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PLI16_01010"
     CDS             <1..589
                     /locus_tag="PLI16_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOH83168.1"
                     /translation="YKNCPAFSAEYKNHFGYPPSKEGCASQPKYQPLKKQTIMKLKEK
                     IINLDPLSVIFVQSIGNYSSNAPEKAWNTLWDFVEKKNLDVEKSEYFGIVHDDTEVTK
                     ATQCRYDACITVPKDVKPEGKIGNKTLDRGKYAVFTHKGPYKTLEKTYDQIFGPWLSG
                     TKEQLADLPVLEKYLNDPFDTKAEDLITEIYVPIV"
     gene            complement(810..1241)
                     /locus_tag="PLI16_01015"
     CDS             complement(810..1241)
                     /locus_tag="PLI16_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83169.1"
                     /translation="MKKREFAVCRRNNITPASYTWDYCVQNKLPYIIVYPKIKFCDIS
                     YDLLSQNKGLAFEKEHEFLDRWLKIYNDYVKRNNFPRLRIKHIGGRLHAIFTIFKKDR
                     EEILDLLFDEIEKHIKKYGYTDEKLSEHYRKIEKFNNRMRR"
     gene            complement(1274..2293)
                     /locus_tag="PLI16_01020"
     CDS             complement(1274..2293)
                     /locus_tag="PLI16_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83170.1"
                     /translation="MESLFEKYEDLIRNLPLEEHSFTTKKKNWKNINPEIIEYHEDIF
                     GQDTEITISRKDLFRSFNNDPIETFILKVIYWGYPAGMRGDNFKKITSPESLFFLKKF
                     LNDIKQNDVIDEKRIGYALKTINGLGLSTLSKYLHFTKSTFMGKKCLILDRELIKIFN
                     NMYFVEFNGLRKVLYPCTVKNYFDYIDISHHIAEYHKFEADRLEMFLFIFGNHLNLEP
                     TGDDGFWDESWDCEPATYEFKKLEGSGRIRVLEISPDDIKKFIDQKISYPLPYSNFNE
                     LYSIIINCWDKYADISSHLLKKEVADGLKSKKIQFAAKKLDEFIHLFMEALSVLYGLH
                     EVDDN"
     gene            complement(2304..3149)
                     /locus_tag="PLI16_01025"
     CDS             complement(2304..3149)
                     /locus_tag="PLI16_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83171.1"
                     /translation="MKVFYNENQSVKSNKSISPSAGKPALLMAELEKLSYIQIESDWP
                     PLSLKDFCLVHERKHVQEILNCKKANGFGNYSKDVSDSLYWTSASFYHAARYAYVNKT
                     VAMSPTSGFHHAGYRSSGGFCTFNGLMISAALLIKEFPDIKIGIIDLDAHFGNGTVEI
                     IERLGLDIRHYTITDYIHELKRKSQFEKMQEFSDRDMIFFQAGADAHIDDPIGGGLTT
                     EELMVRDRTVFEYAKTKNIPLVWNLAGGYQTPVSRVIVLHMNTVKICNEVFENTRNNV
                     LNKKN"
     gene            complement(3265..4296)
                     /locus_tag="PLI16_01030"
     CDS             complement(3265..4296)
                     /locus_tag="PLI16_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83172.1"
                     /translation="MDIYLIRTPEFEPGTYVEVHELLKTSRGPLQFHADDIKFSALNF
                     PFLEHNKKNIPLSWHELFSICDFYRKKHKVRKEEIIILLTNRRNDRNFFSAFDEHNNS
                     FIHAGDLEKFINSPPEFPLAYQVIANILRMLMKISPTLPESWFHRESIGCMNDLCSNK
                     KEIILKLRTGDICLDCLAKMKKEKVDETLINQAIGIFDMLRKRLLFVQGFTGNITPRN
                     VFVDRKGKIVIGDKEILLDYLPKTLFLFFLIHNEGIALNDLEYYRQDMLKIYRILRPG
                     GEESAIKRLSAPYTGDGTFAKNKSLLNKKLRKQLGEPLAGFYCLEGVRGEKYSIGVDP
                     GLVRIELQY"
     regulatory      4404..4501
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            4575..5144
                     /locus_tag="PLI16_01035"
     CDS             4575..5144
                     /locus_tag="PLI16_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83173.1"
                     /translation="MSILCNVRGIISRFKEISGKDYSEWKKILLSKPHYDRRAAYDFM
                     VEQGMKTYIGFLLWTCAYDIKYISKTSRPIPGEEAHFEFLAKVANDAKSQNVLYEKET
                     LYSTFKIEIFYENGNLIVSRWILNDRYEDERSIVIDKKDLSRLLEFLGSGGQNLTAAL
                     KARFCNDTAFEDIQQLLRHANIDYSGRLY"
     gene            5225..5692
                     /locus_tag="PLI16_01040"
     CDS             5225..5692
                     /locus_tag="PLI16_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOH83174.1"
                     /translation="MSKEIERKYFIEKDLWEKVTPDETVLIRQVYLLTEPEISIRIRT
                     KDNEGFITIKGRSQGPVRSEYEYPIPIEDANELIEKYSATLIEKKRHIVYYKNKKWEV
                     DEFLGTNSGLILCEIELESEDQQYEKPEWIDKEVTDDMRYYNSVLSVNPYSKW"
     gene            6054..7553
                     /locus_tag="PLI16_01045"
     CDS             6054..7553
                     /locus_tag="PLI16_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83175.1"
                     /translation="MRNAPLRIIIIILFSEILLVGYSFFYVRTENSYIRDFARFYTPR
                     SLFDFSVQGENTIADSLLEKYLANAENNGQILHRTFLSKIGPKKQNIFLTNPDIVDEQ
                     GKPCGKALDNFFKALLNEKDTAVIRVAHYGDSQLEGDRVSYVVRDKMHTKFGGSGVGY
                     VPLKDLSPVSYVRNSSGNWARYSVFSNKDPEKNYGIAGTVFRFYKYSVRQSDEKPTST
                     DSLSLPDSQPEPQRISYRNASISIKQSNKYSFNTISFLYGRNEGNCAVNIYNTTSGEL
                     VKTDTLPPCATVCFHKIATGPLLNIRVEFVADQSPDFYGMYLDAGRGVQVDNYAIRGH
                     SGDGLMHISDSKLKEMLTLTNTRLVIFQYGANMVPYVRSEKACAWMGDIYYNLFMKFK
                     KAKPDLSILVIGAGDMAHGSEGKYLSYSWLPQITATQKQAALRAGCAYWDLYNMMGGA
                     NSVLIWAQKNLAVTNGHFSSKGQQVVANEIVEALMTEYNKFIYRKPTTK"
     gene            7555..9402
                     /locus_tag="PLI16_01050"
     CDS             7555..9402
                     /locus_tag="PLI16_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH83176.1"
                     /translation="MLHVKKYNGILFSLVPTVILFCAIVSLSGFSKNNTKTEDPALLA
                     AHIDSLVKYRAYYPFIRYEKNYIEWFGSAGIGSFFRKVAQTDTRKLKILHIGDSHIQA
                     DIPSGYVRERLQETFGYGGRGLVFPFKAAGTHAAYDYKTSCSGRWEYNRNIQRDYRYD
                     MGLIGATIHTSDTCASFKLIFREGYIKENFTLLKIYCKQDSLSFDLKIKTSPGEVPVH
                     VDCNDYTNSKPYVLLKMAKATDTLEIFVNKTEAKQNFFECYGLLIENDEDRGVLYNST
                     GINGAGYVSFLRQRIFGQQLQELKPDLVIIDMGANDFNARVYNDAAMENNLVRIINSI
                     RDSAPEAAILISNAQDIYYRRKYNVTQCKDFMQMTQRVAQKYHCAFYNYYTVSGGRRS
                     MDQWYKNGLARKDKVHLSAPGYYIRGELLLNAMLNSYVVWLQQQNDTLIADQHLLDTL
                     ALKKYFDEEINFKTVAAKPLPVAVSNNEENDNEGSDVTYYKIRKGDNLGAIAERFGVR
                     VSQLQQWNALLGTKIIAGATLVIYKKSTTSTPLNIPEKPAGTKPVNHSATVPASSERT
                     VAGKKSYKVVSGDNLWLIAKKFNTTVEKIKQLNGLKTDKLSINQILLIP"
     gene            9411..10907
                     /locus_tag="PLI16_01055"
     CDS             9411..10907
                     /locus_tag="PLI16_01055"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOH83177.1"
                     /translation="MLDFSLNDFLSGLLYRPQTPMVFNSMLFVTLFTLFYLVYVVVYK
                     KIAVRNVLLLLFSLYFYYKISGFYVVSLVLIASSDFFIGRLMFVKATKKSRRNLLLLS
                     LFINLGLLIFFKYTNFFLNAYFGITAGETSPYVLSLITPVGISYFVFKTLSYIIDIYK
                     KNIAAPETNLVNYLLYVSFFPNILAGPILRARDLLPQFRQALDLSRAFVGKALLLILV
                     GVFKKVVIADFLGVNLVDRVFDTHVYFSSFEYLMVGYGYIVQLYFDFSGYTDMVIGIA
                     MLLGFSSAPNFNKPFMAQNISEFWRRWHITLSNWLRDYIFTPMSIRLRNLQNAGLAIS
                     VLATFIICGLWHGANYTFLVWGTLHGLIMGYEVLSRRIRMKVRKKVNPLLYKMLSIFI
                     TVHVLMFTFIIFRAPDIQVAADMLQKIFGGVDFSLAGQWFGFYKYPFFVMLAGILLHY
                     TPAVWNTRLTDFFGRLHWTIQGIVIFVAVIFIYQFFSTEAQPFIYLNF"
     gene            10917..>11515
                     /locus_tag="PLI16_01060"
     CDS             10917..>11515
                     /locus_tag="PLI16_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH83178.1"
                     /translation="MQPFKITCNQYNIAYYCTRYQCLNGNSQKLAFRYIGHKGDSCDY
                     SFEDLDGQSNRYANLLTQLGIKKGDIVFTFLPKMPEQFFAFLGILKTQAVAGTLYSNF
                     GEEALADRLGDSRAVAVITKKSLLKKLMRALPQLEHLKYILVVDGTENDEKALALPPM
                     LSNASDKFEVSVTGPDAPSVLHYTSGSTGKPKGVQHVHK"
BASE COUNT         3350 a         2443 c         2297 g         3425 t
ORIGIN      
        1 atacaaaaac tgtccggcct tttcggctga atacaaaaac catttcggct acccgccctc
       61 aaaggaagga tgcgcatcac aacccaaata tcaaccctta aaaaaacaaa ccatcatgaa
      121 attaaaagaa aaaatcatca accttgatcc gctgtctgtc atctttgtgc agtccatcgg
      181 aaattatagc agcaatgccc ctgaaaaagc gtggaacaca ttatgggact ttgttgaaaa
      241 gaaaaacctt gatgtggaga aatcagaata tttcggcatt gtccacgatg acaccgaagt
      301 cactaaagcc acccaatgca gatacgacgc atgcataacg gttcccaaag atgtcaaacc
      361 cgaaggcaag attggcaaca aaaccctcga cagaggcaag tatgcggttt tcacgcacaa
      421 aggcccttac aaaacgctgg aaaaaactta tgaccagatt ttcggcccct ggctttccgg
      481 tacaaaagaa cagcttgccg accttcccgt attggaaaaa tatctcaatg atccttttga
      541 cactaaggcg gaagatctta tcactgaaat ctatgttcct attgtgtaat aattgacagg
      601 gcaggttcct ttccaaaggg cttgcccttt ttattttttt atggaacacc tacgtcaaaa
      661 gttgatttat ttttcagcaa aaacaaaaga gctggaatcc tgcggtggtc ctgaagaaaa
      721 tggcgccttg tttgaagaag ttttaaccat taaaattaat tttgattaaa actgaatttt
      781 agtttcaact gttatttata catgacaaat tatcgcctca ttcggttgtt gaatttttcg
      841 attttccggt aatgctccga tagtttttca tcggtgtatc cgtacttttt tatatgtttt
      901 tcaatttcat caaaaagcag gtcaagaatt tcctcccgat cttttttaaa tatggtaaag
      961 attgcatgta gcctcccgcc tatatgcttt atcctgagcc tggggaaatt gtttctttta
     1021 acataatcgt tgtaaatttt caaccaacgg tccaaaaact catgttcttt ttcaaatgca
     1081 agtcctttat tctgagatag caggtcataa ctgatatcgc agaattttat tttggggtaa
     1141 acaatgatgt atggaagttt gttctgaaca caatagtccc aagtgtatga agcaggcgta
     1201 atattgtttc tcctgcagac agcgaattct ctttttttca tgttttatta attttttagt
     1261 tcctgaaatt attttaatta tcatcaacct catgtagtcc atacaaaaca gacaatgcct
     1321 ccataaacaa atgtatgaac tcatccagct tttttgccgc aaattgtatt ttcttcgatt
     1381 ttaatccgtc tgcaacttcc tttttcagta aatgactgct gatatcagcg tatttgtccc
     1441 agcaattgat gataatggaa tacaattcat tgaaatttga atacggcagc gggtaactga
     1501 ttttttgatc tataaatttt ttaatatcat ccgggctgat ctccaaaacc ctgattcttc
     1561 cggacccttc aagttttttg aattcgtaag ttgcgggctc acagtcccag ctttcatccc
     1621 aaaaaccatc atcgcctgtt ggctcgagat ttaaatgatt cccgaatata aaaagaaaca
     1681 tctccaggcg gtcagcctca aatttatgat actccgcaat atggtgcgaa atatcaatat
     1741 aatcaaaata atttttcacc gtacaggggt acaaaacctt ccgcaagcca ttgaattcca
     1801 caaaatacat attattgaaa atctttatca gctccctgtc aaggatcagg cactttttcc
     1861 ccataaaggt ggattttgtg aaatgcagat atttgcttaa tgtcgaaagt cccagcccgt
     1921 ttattgtttt gagtgcataa cctattcttt tttcatctat cacatcattt tgctttatat
     1981 cgttgagaaa ttttttcaaa aaaaataagc tttccggact tgtgattttt ttaaaattat
     2041 ctcctctcat tcctgccggg tatccccaat aaataacttt cagaatgaac gtttctatcg
     2101 ggtcgttgtt gaatgaccgg aacagatcct ttctgctgat tgttatttct gtgtcctgac
     2161 caaaaatatc ctcatggtac tcaataattt cagggttgat gtttttccag tttttctttt
     2221 ttgttgtaaa agagtgctct tccaatggaa gatttcttat taaatcctca tatttttcaa
     2281 atagactttc cattacttat agtttaattt tttttgttta gtacattatt ccgggtattt
     2341 tcaaatactt cattacagat tttcaccgta ttcatatgca atacaatgac ccttgaaacc
     2401 ggcgtctggt accctcctgc aaggttccat accaaaggaa tatttttcgt ttttgcatat
     2461 tcaaagactg ttctatccct taccattaat tcctcagtgg taagcccacc gccaatggga
     2521 tcgtcaatat gtgcatctgc acccgcctga aagaaaatca tatccctgtc agaaaattcc
     2581 tgcatttttt caaattggct ttttctcttc aattcatgaa tgtagtcggt aattgtataa
     2641 tgcctgatat ccaggcctaa tctttcaata atttccactg tgccattgcc gaaatgtgca
     2701 tccaggtcga tgatgccgat tttgatatcc gggaactcct ttatcaacag agcagcactt
     2761 atcatcagtc cgttaaaagt acagaatccg ccagaggagc gataacccgc atgatgaaac
     2821 ccggaggttg gtgacatcgc taccgtttta ttgacataag catatcgcgc agcatggtaa
     2881 aatgaggccg acgtccagta caacgaatct gaaacgtctt ttgaataatt tccaaaacca
     2941 tttgcttttt tgcaattcag gatttcttgt acatgctttc tttcgtgaac aaggcagaaa
     3001 tcttttaatg aaagcggagg ccagtctgat tcaatctgaa tataggatag tttttccagt
     3061 tctgccatca gtaaagccgg ttttcctgca ctcggggaga tgcttttatt gctttttacc
     3121 gattgatttt cattgtaaaa tactttcatg gtgctttcct ttgtttttag tggtttaatt
     3181 ttctaaaaac aaaattagaa cagcacggag cagaataaaa gatatgtaac gttacaaagt
     3241 tcagagaact aagtcacaaa agcatcaata ttgaagttca atgcggacta acccggggtc
     3301 tacaccaata ctatattttt cacccctgac tccctcaagg caataaaaac cggcgagcgg
     3361 ctccccaagc tgttttctta attttttatt gagaaggctt ttgttctttg caaaggtacc
     3421 gtctcctgtg tagggcgccg ataggcgttt aattgctgat tcttcgccgc cgggtctcag
     3481 gattcgatag attttcagca tgtcttgtcg gtagtactcc agatcgttca gggcgatgcc
     3541 ctcgttatga attaagaaaa acaggaacag tgtcttaggc aaataatcca atagtatttc
     3601 tttatcccct attactatct ttccttttct atcaacaaat acattgcggg gagttatatt
     3661 tcctgtaaag ccctgtacaa acagcaagcg ttttctcagc atatcaaaaa tgccgatggc
     3721 ctgattaatc aacgtttcat ccactttctc ttttttcatt ttagccaggc aatcaaggca
     3781 gatatcgcct gttctgagtt tcaggataat ctcttttttg ttcgaacaca agtcgttcat
     3841 acagcctatg gattcccggt gaaaccatga ttcgggcaat gtaggcgata ttttcatcag
     3901 cattctcagg atgttggcta taacctggta tgccaatgga aactcaggcg gtgaattaat
     3961 aaatttttca agatccccgg catggataaa agagttattg tgttcatcga aggcgctgaa
     4021 aaaattcctg tcatttcttc tgtttgtcag caatatgatg atttcttctt ttcttacttt
     4081 atgttttttc ctgtaaaaat cacagatgga aaacaattca tgccagctta acgggatgtt
     4141 tttcttattg tgttccaaaa acggaaaatt cagggcacta aatttaatat catctgcatg
     4201 aaactgcagt ggacccctgg atgtcttcag caattcatga acttcaacat aagtgcctgg
     4261 ctcaaattcc ggagtcctga taagatatat gtccatttta tttagagtta ttgagagtct
     4321 ttaactgcat ttcaaagtta ggaaaacaat tgaatttata aatttacttt tatttgctat
     4381 aagaatattt ttttgtatat ttgcctcagc attaagagat cccggggaag ggacggcaac
     4441 cgagtaagaa caccacggtg ccaaaacaat gcgcagcttc tgctgaaaaa agttctgaat
     4501 cgctattgca tcatcttccc gtagtttctc tcaatgtgaa ataaattatt gtttaacaat
     4561 taaatttttt cactatgtca attttatgta acgttagggg aatcatttca agattcaagg
     4621 aaatttcagg caaggattat tccgaatgga aaaaaatcct tttaagcaaa cctcattacg
     4681 acaggcgtgc cgcttatgac tttatggttg agcaggggat gaaaacttat atcggatttt
     4741 tactctggac atgcgcttac gatattaaat acatctcaaa aacatccagg ccgatacccg
     4801 gtgaagaagc tcactttgaa tttttagcaa aagttgccaa tgatgcaaaa agccagaatg
     4861 tgctttatga aaaagaaacg ctttactcaa cttttaagat tgaaatcttt tatgaaaacg
     4921 gaaatttaat agtttccaga tggattttaa acgatcgtta tgaggatgaa agatctattg
     4981 tgattgataa aaaagatttg tccagactcc tggaatttct tggctctggc ggtcagaacc
     5041 ttactgctgc tctaaaagca aggttctgta atgataccgc ttttgaggat atacaacagt
     5101 tattaaggca tgcaaatatc gattacagtg gtaggttata ttgatttgtc aacattgaac
     5161 caatctgaaa aaacaatggg aagatgataa ataaaaattt aatattgtaa aaataattat
     5221 agttatgtca aaagaaattg aacgtaagta ttttatagaa aaagaccttt gggaaaaagt
     5281 aacccccgat gaaactgttt taataaggca ggtgtatttg cttaccgagc cagaaatatc
     5341 catacgtatc cgaacaaaag ataatgaagg atttattacc atcaaaggca gatcacaagg
     5401 cccggtaagg agtgaatacg aatatcccat tcctatagag gatgctaatg agctgatcga
     5461 aaagtattct gcaaccctaa tcgaaaaaaa gagacatatc gtttattata aaaacaaaaa
     5521 atgggaagtg gatgagtttc tgggaacaaa ttcaggattg atcctttgtg aaatagagtt
     5581 ggaaagcgaa gaccagcaat acgaaaagcc cgaatggatt gacaaggagg tcacagacga
     5641 tatgcggtat tataattcgg ttctttcagt gaatccttat tccaagtggt aattgttgag
     5701 ttttcttgac aatctgaaag atttcaaaga aatctgggat aatattcaca gggcactaaa
     5761 gcaacaagta ttatcccatt ttttaaaatg taacatcaga atatgaacct gaagttaata
     5821 tctcaataaa tcttcaccgg cacttcctta attgcttccc tttataatag gactgtatta
     5881 catggccgaa atgttctata atatcatatt tttccgcccc gcatttccgt ttggtcaatt
     5941 cccaaaaaag tccgaccttt gcatgttttt ttaatcaaca ggcataaccg gctagaagat
     6001 aacccaaaaa ctacataacc gtaagccggc ccgccgatag taaacattct cacatgagaa
     6061 acgcgccatt acgaatcatc atcatcatac tgttcagcga aatcttactc gttggatact
     6121 cgttttttta tgttcgcacc gaaaacagct atatcaggga tttcgctcgt ttttacactc
     6181 cccgttcgct atttgatttt tctgtacaag gcgagaacac cattgccgac agcctgttgg
     6241 aaaagtacct tgccaatgcc gagaacaacg gtcagattct tcatcgtact ttcttgtcga
     6301 agataggccc caaaaaacaa aatatctttt tgaccaaccc cgatatcgtt gacgagcaag
     6361 gtaaaccctg tggcaaggcg cttgacaatt ttttcaaagc cctgctcaac gaaaaggata
     6421 cagcagttat ccgtgttgcc cattatggcg attcgcagct cgaaggcgac cgggtgtcgt
     6481 atgtggtacg cgacaaaatg cacactaaat tcggaggttc cggcgtgggc tatgtgccgc
     6541 tgaaagacct cagccccgtg agctacgtgc gcaacagcag cggcaactgg gccaggtatt
     6601 cagtatttag caacaaagac ccggaaaaaa attacggcat cgccggaacg gttttcagat
     6661 tttataaata ctcggtgcgg caaagcgacg aaaaaccaac atccacagac tctttatctc
     6721 tgcccgattc acagcccgaa ccacagagaa tttcgtaccg caatgccagc atctccataa
     6781 aacagagcaa caaatacagt ttcaacacca tctcgtttct ttatggacgc aacgagggta
     6841 attgtgctgt taatatctat aacaccacct ccggcgaact cgtaaaaacc gacaccctgc
     6901 cgccatgtgc taccgtatgt tttcacaaaa ttgccactgg cccgctgctg aatatcaggg
     6961 tggaatttgt tgccgaccag agtcccgatt tttatggtat gtacctggat gccggccgtg
     7021 gtgtgcaggt tgacaactat gccattcgcg gacattccgg cgacgggttg atgcatatca
     7081 gcgacagcaa actgaaagag atgctgacac tgaccaacac ccgcctggtg atttttcagt
     7141 acggcgccaa tatggtgcct tacgtgcgtt ccgaaaaagc ctgtgcctgg atgggcgaca
     7201 tatattacaa cctgttcatg aaattcaaaa aagccaaacc cgacctgagc atactggtaa
     7261 tcggagccgg cgatatggca cacggcagcg aggggaaata tctctcctat tcgtggttac
     7321 cgcaaattac cgctacacaa aaacaggcgg ccctcagggc aggatgcgct tactgggatc
     7381 tctataatat gatgggcggc gccaattcgg tgctcatctg ggctcaaaaa aacctggccg
     7441 ttaccaacgg acatttttca tccaagggtc aacaagtggt ggccaacgaa attgtggaag
     7501 ccctgatgac cgaatacaat aaattcatct accggaaacc aacaacaaaa tagtatgttg
     7561 cacgtcaaga aatataacgg catcctgttt tcgttggttc ctacggtaat actgttttgt
     7621 gccatagtgt cgctgagcgg tttttcaaaa aacaacacca aaaccgaaga ccctgctctt
     7681 ttggctgcac atattgactc cctcgtgaag tatcgcgcct attatccttt tatccggtac
     7741 gaaaaaaact atatcgaatg gtttggttca gcaggtatcg gcagtttttt ccgtaaggta
     7801 gcccaaaccg acacgcgcaa attgaaaatt ctgcatatcg gcgattcaca tatacaggcc
     7861 gacattcctt cgggctatgt gcgtgaacgg ttgcaggaaa catttggcta cggcggccgg
     7921 ggactggtgt ttccttttaa agcggccggc acacatgctg cctacgacta caaaacgtcc
     7981 tgttcgggaa gatgggaata caacagaaat atccaacggg attatcgtta tgatatgggc
     8041 ctcattggcg ccaccataca tacttcggac acctgcgcat cgtttaaact tatttttcgc
     8101 gaaggatata tcaaagaaaa ctttaccctg ctgaaaatat actgcaaaca ggattcgctg
     8161 agtttcgacc tgaagataaa aacttctccc ggcgaagtgc ccgttcatgt ggattgtaac
     8221 gattatacca acagcaaacc ttatgtattg ctgaaaatgg ctaaagccac ggacaccctg
     8281 gagatttttg tgaacaaaac ggaagcaaaa cagaactttt ttgaatgcta tggcctgttg
     8341 atcgaaaacg atgaagacag aggtgtgctg tacaatagca ccgggataaa cggcgccggc
     8401 tatgtgagtt ttttgaggca gcgcattttt ggacagcaac tgcaggaact caagcccgac
     8461 ctggttatta tagatatggg tgccaacgac tttaacgcca gggtgtataa cgatgccgcc
     8521 atggagaaca atctggtgag gattatcaac agtattcgcg atagtgctcc cgaagctgcc
     8581 attcttatct ccaatgctca ggatatctat tatcgccgca aatacaatgt gacccagtgc
     8641 aaagatttta tgcaaatgac acaaagggtg gcacaaaaat accattgtgc attttataac
     8701 tattataccg tcagcggtgg ccgcaggtct atggaccagt ggtacaaaaa cgggctggcc
     8761 cgcaaggata aagtgcatct ttcggctccg ggatattata tcaggggaga gttattatta
     8821 aatgccatgt taaattctta tgtagtgtgg ctgcagcaac aaaatgacac cctcattgcc
     8881 gaccagcacc tcctcgatac cctggcactg aaaaaatatt ttgatgaaga aataaatttc
     8941 aaaacggtag ccgccaagcc cttgcctgtg gccgtaagca ataacgaaga aaatgataac
     9001 gaaggatcgg atgtaaccta ttataaaatc cgcaaaggcg acaacctggg cgctattgca
     9061 gaacgatttg gcgtgcgggt gagccagttg cagcaatgga acgccttatt gggaacaaaa
     9121 atcattgccg gtgcgacact ggtgatttac aaaaaatcaa ccacttcgac tcctttaaac
     9181 atacccgaaa aacccgcggg gacaaaaccg gtcaaccatt cggccactgt gcctgcctca
     9241 tcagaaagaa ccgttgccgg gaaaaaatcc tataaagtgg tttccggcga caacctttgg
     9301 cttatcgcca aaaaattcaa taccaccgta gaaaaaataa aacaactcaa cggactgaaa
     9361 acagataaac taagtattaa tcaaatacta ttaatcccgt aagactattt atgcttgatt
     9421 tttcgctcaa cgatttttta tctggattgc tctatcgccc gcaaacgccg atggtgttca
     9481 actccatgct gtttgtcacc ctgtttacgc tgttttacct ggtatatgtc gtggtttata
     9541 aaaagatagc tgtccgcaat gtgttgttgc tgttgttcag cctgtatttt tattacaaaa
     9601 tcagtggttt ttatgttgtt tcgctggtgc ttatcgccag cagcgatttc ttcataggca
     9661 ggcttatgtt tgtaaaagct accaaaaaat ccagaagaaa cctcctgttg ttatcattgt
     9721 ttatcaatct ggggttgctt atctttttca aatacaccaa tttcttctta aatgcatact
     9781 tcggtattac tgccggagaa acatcacctt atgttctgag cctgattacg cctgtgggca
     9841 tttcttattt tgtgtttaaa accctctcct acataattga tatttacaaa aaaaatatcg
     9901 ctgcgccgga aactaattta gtcaattacc tgctctatgt gtcatttttt cccaacatac
     9961 ttgccgggcc catattgcgt gccagggacc tgttgccaca gttccggcag gcgcttgacc
    10021 tcagccgtgc atttgttggc aaagccttgt tgctgatcct tgtcggtgtc tttaaaaaag
    10081 tggttatcgc tgattttttg ggtgtcaatt tggtggatag ggtgttcgat acgcatgtgt
    10141 atttttcatc ttttgagtat ttgatggtcg gatacggcta cattgtacag ttgtattttg
    10201 atttttcggg ctataccgat atggttatcg gcattgccat gttgctcgga tttagcagcg
    10261 cccccaactt caacaagcca tttatggcgc aaaacatttc ggagttctgg cggaggtggc
    10321 atatcaccct gtctaactgg ctacgcgact atattttcac gcccatgtcg atacgtctca
    10381 gaaatttaca gaatgcaggg ctggcgatct ctgtgctggc tacctttatt atctgtggtt
    10441 tgtggcatgg cgccaactat acttttttgg tgtgggggac gttgcatggc ctgataatgg
    10501 gctacgaggt gctgtcacgc cggatacgga tgaaggtgag aaaaaaggtt aaccctttat
    10561 tgtacaaaat gctgagcatc tttattaccg ttcatgtgct gatgtttaca tttatcattt
    10621 tcagggcacc cgatatacag gtggctgcag acatgctgca gaaaattttc ggaggtgtgg
    10681 acttttcact ggccgggcaa tggtttggtt tttataaata tccctttttt gttatgctgg
    10741 ccggtatcct gttgcattat accccagccg tttggaacac ccggcttacc gactttttcg
    10801 gacgcctgca ctggacaatt cagggaatag tgatctttgt cgcagtgatt ttcatttatc
    10861 agtttttcag taccgaggcc caaccgttta tttatcttaa tttttaatca cacatcatgc
    10921 agccctttaa aatcacatgt aatcaataca acattgctta ttactgtacc cggtatcagt
    10981 gtctgaacgg aaacagccaa aaacttgcct ttcgttatat cggacataaa ggggatagct
    11041 gcgattattc atttgaggat ttagatggtc aaagcaaccg ctatgccaac ttactcacgc
    11101 aactgggaat aaaaaagggc gatattgttt ttacgttttt gcccaaaatg cctgaacagt
    11161 tttttgcatt tttaggcata ttaaaaacac aggctgtggc aggtacactg tactccaact
    11221 ttggggagga agcccttgcc gaccggcttg gcgacagcag ggcagtggcg gttattacca
    11281 aaaaaagcct gctgaaaaaa ttaatgagag ccttgccgca gttggagcat ctaaaatata
    11341 ttctggtggt ggatggtacc gaaaacgatg aaaaagcttt ggcgctaccg cccatgcttt
    11401 ctaatgcttc ggacaagttc gaagtgtcgg ttacaggtcc cgatgctccg tcggtactgc
    11461 attatacttc gggttctacc ggcaagccta agggcgttca gcatgtccac aaaag
//
LOCUS       DAOSFP010000017        11409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4790_length_11409_cov_4.030738, whole genome shotgun sequence.
ACCESSION   DAOSFP010000017 DAOSFP010000000
VERSION     DAOSFP010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_11409_cov_4.030738"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="PLI16_01065"
     CDS             <1..1033
                     /locus_tag="PLI16_01065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83179.1"
                     /translation="FCRKKKSTQSCLPVSVVICARNEYYNLEKNLPLILEQDYPDFEV
                     IVVNDESDDDTNDLLCDLAIKYPRLVVLKTQKNKNCFPGKKFSLSLGIKSAHNDIVLL
                     TDADCAPKSPDWIRQMQAPFSNSKISIVLGYGAYAQKKGLLNKLIRFDTVSIALLYFA
                     MAKAKMPYMGVGRNLAYRKSLFIQSKGFVSHYHISSGDDDLFINQNATCKNTAIVFSP
                     ESHTVSEPKTNYSSWVLQKKRHLTTGRYYKFRHKLLLTLWPATTVAFYLLAAAIMVFN
                     CNLYNIIAVCTFLLLRVISQLFIYKKAMNKLRERKFLLYLPFFEVFFIIFNLLVMVSN
                     TGKQQSKWK"
     gene            1024..1530
                     /locus_tag="PLI16_01070"
     CDS             1024..1530
                     /locus_tag="PLI16_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH83180.1"
                     /translation="MEVNTNLTEKAQRDYRLVQLAIRNGDQKAYAELMGYYKDAIYFL
                     LLKMTNDPDDADDLTIEAFGKAFKKLDQYTPDYAFSTWLFKIASNNCIDFIRRKRMDM
                     LSINKTVVDDEGTDMSQVLPAHGLDPEEKLIERQKIMMMREVVEKLKPNYRRLVQLRY
                     FDEYSYEE"
     gene            complement(1726..2172)
                     /gene="tnpA"
                     /locus_tag="PLI16_01075"
     CDS             complement(1726..2172)
                     /gene="tnpA"
                     /locus_tag="PLI16_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOH83181.1"
                     /translation="MSSYTQIIYQIVFSTWSREKTLSENNREELFKYIAGILKNKKCH
                     LYRINGIADHIHIVTHLHPSVALADLIKDIKLASSGWIKANNIFKNFNGWQAGYGAFT
                     YSIIDKAHLIEYVKNQNEHHRIKTFTEEYIDLLKEQGIDFDEKYLF"
     gene            complement(2964..3743)
                     /gene="rsmA"
                     /locus_tag="PLI16_01080"
     CDS             complement(2964..3743)
                     /gene="rsmA"
                     /locus_tag="PLI16_01080"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOH83182.1"
                     /translation="MTFVYPKKFLGQHFLKDESIALQICESLTLHNNYRQVLEIGPGT
                     GILTQHLWASKNFQTTLIELDREAYTFLIEKYPDRKNQIIHGDFLHYNLNNIFDEPYA
                     IIGNFPYNISTQILFKVFENRDMVPEVVGMFQKEVAQRIASPPGSKQYGILSVLLQAF
                     YTIEYLFTVDEHVFYPPPKVKSAVIRLRRNDTKSLNCDEKLFVDIVKKAFNQRRKTLR
                     NALSSFRNEENNLSDPIFDKRAEQLGVSAFVDITRRFSGLV"
     gene            complement(3745..5616)
                     /locus_tag="PLI16_01085"
     CDS             complement(3745..5616)
                     /locus_tag="PLI16_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83183.1"
                     /translation="MPDQHKQAKHPCRLWSFVRLPVLVFFLLTTAYQGAAQPGTNEQL
                     AIQYFQDKEFEKAVVLFEELYNKTSTPFIYDYYLNCLVELNQFDKAEKLINKAIKKNP
                     GNYALSVDLGYVYEQEKQHEKAKKQFENTVKSLISDKEQIVITANAFLLRNQPDYAIK
                     TYEQGKKLLRNTYLFNLELAEIYLKKNDFSAALDQYLEYVKNNPGSQEEVQSQLQDLL
                     ANDADNTRNTIFRTVLLQRIKKEPDIRPYPLLLLWYFIQEKEFGAAAVQAKAIDKRFD
                     EEGERPYELAAIAVSNGFYDDAISCYDYILSTKDIYSPYFQSALVEKMNTKYLKATST
                     PNISLQELELLEKEYFDLLSKNKDNNYSLRLIKNLAHLQAFYMDKPAQGAELLQKAIQ
                     YTGVSAELIAQCKIELADILLLTGDVWEASLLYSQVEKAFKNDVVGFEAKFRNARLYY
                     YIGEFDYARAQLDILKAATSKLIANDAMQLSLLISDNMDEDSTTTGLRLYSRAELLIF
                     RNKYDEAFKTLDSINTLGLYHPLFDEVLFQKAGIRLRQGRYADADSLLVKLVNFYQQD
                     ILADEALFKLGELNENIFKNYTRAMEFFERILTDYPGSIFVVEARRHYRNLRGDKIN"
     gene            complement(5621..6214)
                     /locus_tag="PLI16_01090"
     CDS             complement(5621..6214)
                     /locus_tag="PLI16_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83184.1"
                     /translation="MKLLTNSTIMLRTPFILAVAIGLLALSSCHNAEKKQQIAKIDSL
                     ITIIDSTESNLEKMNIDTVKKKYNDFKETNKRVIVHYHEYRNDENWKYMCAFQEVRKA
                     FKVMVNKHKSYQQELKISREQLENLRHDVDKSLLEEKEFKDYFTMESKSVHDLTFQIN
                     RQINQVLQQYANFDTVHPYLLKLLETYPKDDSPAEKK"
     gene            complement(6198..7475)
                     /gene="serS"
                     /locus_tag="PLI16_01095"
     CDS             complement(6198..7475)
                     /gene="serS"
                     /locus_tag="PLI16_01095"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOH83185.1"
                     /translation="MIQLSSLRHDAENIVKALAIKNFDATQLVADILACDDERRATQK
                     KLDDLLAELNKMAKETGELFKNGKHQEANLLKEKSIELKTTVKELEDNLKTSEEKQKN
                     LLLQLPNTPHPSIKPGKSAEDNEIVFTGGADIPALNPGNDLPHWELASKYKLIDFELG
                     NKITGAGFPVYLGKGARLQRALIQFFLDSAVNAGYLEIEPPLMVNEASGYGTGQLPDK
                     DGQMYYAPEDKLYLIPTAEVPVTNLYRDMILKESDLPLKNVAYSSCFRREAGSYGKDV
                     RGLNRLHQFDKVEIVQIQHPEKSYDTLKEMCDYVANLLVQLELPFRILKLCGGDISFT
                     SAMTFDFEVFSTAQKRWLEVSSVSNFESFQANRMKLRYKNKDGKINLVHTLNGSALAL
                     PRIVAALLENNQTADGIRIPAALTRYTGFEIIN"
     gene            complement(7570..7851)
                     /gene="rpmA"
                     /locus_tag="PLI16_01100"
     CDS             complement(7570..7851)
                     /gene="rpmA"
                     /locus_tag="PLI16_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOH83186.1"
                     /translation="MAHKKGAGSSTNGRESHSKRLGVKIYGGQLATAGNIIVRQRGTV
                     HNPDQNVGIGKDHTLFALTDGIVEFQKKKNNRSYVSVTPLEKQDTEKIQ"
     gene            complement(7881..8192)
                     /gene="rplU"
                     /locus_tag="PLI16_01105"
     CDS             complement(7881..8192)
                     /gene="rplU"
                     /locus_tag="PLI16_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOH83187.1"
                     /translation="MYAIVDIAGQQFKVEKEQEIFVHRLEGEEGTAVTFDKVMLIDNE
                     GAVKVGTPTITGASINAKILSHMKGDKVIVFKKKRRKGYQKSNGHRQYMTKIQIEDIL
                     V"
     gene            8349..8507
                     /gene="rpmH"
                     /locus_tag="PLI16_01110"
     CDS             8349..8507
                     /gene="rpmH"
                     /locus_tag="PLI16_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOH83188.1"
                     /translation="MKRTFQPSQRKRKNTHGFRKKMESANGRRVLANRRAEGRHKLTV
                     SDEKQHKK"
     gene            8598..8849
                     /locus_tag="PLI16_01115"
     CDS             8598..8849
                     /locus_tag="PLI16_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83189.1"
                     /translation="MGFFFWFLIIVFIGWILFLYVIPFFARLYLKKLAKKFENHSQQT
                     QAPEKKEGSVNIDYIPEKHLEKDKEAPGDYVNFEDIEEK"
     gene            8857..>11409
                     /locus_tag="PLI16_01120"
     CDS             8857..>11409
                     /locus_tag="PLI16_01120"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOH83190.1"
                     /translation="MKNFDYKKFIPYVVAIVVFIAISLIYFSPLLEGKQLYQSDIVNY
                     RGSAQEIKDFRQATGEEALWTNSMFGGMPAYQIDVSFKTNIAKVFNNIFKLWLPHPAS
                     ILMVLLICFFILVLTLKKDPWLAIVGAVAFAFSSYFFLTISVGHNAKTLAIAYMPAVL
                     AGIITAYRGKYLLGAALTALFMAIEIAVNHVQMTYYLMMILGFVVLFEFIYAILKKNL
                     ANFWKATAVIVVAGCISLIPNLSNLWTSYEYMKETIRGKPELTSNQEIRSSGLDKDYI
                     TQWSYGVGETFTLMIPNVKGGGDAMLGESKSALDKVDRNLRKQISQQNHYWGNMPFTA
                     GPVYAGAFVCFLFVLGLFIVKGKLKWALFGVTVLAIMLAWGHNFMPLTDFFIDYVPFY
                     NKFRAVSSILIIAEVTIPILAILALKEIADNPGLIKEKKTAFFVSLGLTAGLTLLFVL
                     LPKMFFDFIKDTEAVQFDGYVQQGANQAQIDDFISNLEIARISIFRMSCLRTILFIVL
                     GAGMLWAYASAKKISKYILYAGVGVLILIDMVPVDKKYLNNDNFIAKNKNENPFVESA
                     ADQAILADTSAAYRVLNLTVGNFTTDATTSFYHQSIGGYHAAKLRRYQDLIEHRLFDE
                     HGQLVKTLQKTPTDSALKATMIGLTSLNMLNTRYFIYNPDAQPLRNPYAMGNAWFVNE
                     VKMVANADEEIKALNNFVPAATAIVDKRFESELGGLKIARDSGAAIRLVKYTPNHLTY
                     ETSKLTSDQLAVFSEIYYNKGWNAYIDGQAKPYVRANYVLRAMVVPAGSHKIEFKFEP
                     QSYVTGEKVSLAGSVLLLLIIVAALYFEFRKKKGAPAEQREIPDEVPVASVAA"
BASE COUNT         3037 a         2290 c         2485 g         3597 t
ORIGIN      
        1 attttgtcgc aaaaaaaaat ccacacaatc ttgtttacct gtatccgtgg tgatatgcgc
       61 ccgtaatgaa tattacaacc tggagaaaaa cctgcccttg attcttgaac aggattatcc
      121 cgatttcgag gtcatcgttg tcaacgatga atcggatgat gataccaacg acctgttgtg
      181 cgacctggca ataaaatatc ccaggctggt cgtattgaaa acccagaaaa acaaaaattg
      241 ttttccggga aaaaaattct ccctctcact gggtatcaaa tctgcccata acgatattgt
      301 gttgctgacc gatgccgact gcgctcccaa aagtcccgac tggatcaggc aaatgcaggc
      361 gccgttttct aattccaaaa tcagcattgt tctgggctat ggcgcttatg ctcaaaagaa
      421 aggccttctg aacaaactca tacgtttcga tactgtatcg atagctttac tgtactttgc
      481 tatggctaaa gccaaaatgc cctatatggg cgtgggccga aatcttgcct accgcaaaag
      541 cttgtttata cagtccaagg ggtttgtcag ccattaccac atcagttccg gcgatgatga
      601 tctgtttatc aaccaaaatg ccacctgcaa aaatacagct attgtttttt cgccggaaag
      661 tcacaccgtt tcagagccaa aaaccaatta tagttcctgg gtgctacaga agaaaaggca
      721 tctgactacc ggcaggtact ataaattccg gcataaatta ttactgacat tgtggcccgc
      781 tactacggtg gctttttatt tattggctgc agctatcatg gttttcaact gcaatttgta
      841 taatattatt gcggtgtgta cgtttttatt attgcgtgtg attagccagc tatttatata
      901 taaaaaagcg atgaataaat tgcgggaaag gaaattttta ttatatttgc ctttttttga
      961 agtgtttttt attattttca acctgttggt catggtttcc aatacaggaa aacaacaaag
     1021 caaatggaag taaacaccaa ccttaccgag aaagcgcaac gtgattacag gctggtgcaa
     1081 ttggctatca gaaatggcga tcagaaagca tatgccgaac ttatgggcta ttataaagat
     1141 gccatttatt ttttgttgct gaaaatgacc aatgatcctg atgatgctga tgacctgact
     1201 atcgaagcct ttggcaaagc ttttaaaaaa ctcgatcaat atacccctga ttatgctttt
     1261 agcacctggt tgtttaagat agcttccaat aattgtattg attttatccg ccgcaaaaga
     1321 atggatatgc tttccatcaa caagacggtg gtggatgatg aaggcaccga tatgtcgcag
     1381 gttttgccag cccatggcct cgatcccgaa gaaaagctta tcgaacgcca gaaaatcatg
     1441 atgatgcgcg aagtggtcga aaaactaaaa cccaactacc gccgcttggt gcagttgcgg
     1501 tatttcgacg aatactccta cgaagaatag cacaggagat gtcgctgcct attggcactg
     1561 taaaggccca gttgttcaga gcccgcgagt ttctgtacaa cattatgaag aatgcggagg
     1621 gatcaatata gtagttcgta gcaccgaaag tacttaagat ataataatat attaaatacg
     1681 ccggaaaaga ggtgtgaaac attcaactgc ttaggagttg aatgtttaaa acaaatattt
     1741 ttcatcaaaa tcaatcccct gttcctttag caaatcaata tattcttcag taaacgtttt
     1801 tattctatga tgctcatttt ggttcttaac atattcaata aggtgggcct tatctattat
     1861 tgagtatgta aaagcgccat atccggcttg ccacccgtta aagtttttga aaatgttgtt
     1921 tgctttgatc cagccggaac ttgcaagttt tatgtctttg ataagatctg ctaatgccac
     1981 cgaaggatga aggtgtgtta caatgtgaat atggtcggct attccgttaa ttcggtatag
     2041 atggcatttt ttgtttttca ggatccctgc aatatattta aacagctctt cccggttatt
     2101 ttcagataga gttttttcac gcgaccatgt tgaaaaaaca atttgataaa taatttgtgt
     2161 gtaagaactc atgatattgg ttttattcaa tccttacatc actgccaggc agggtttcag
     2221 gattgggttt gtattatctt ttatttctgt gcactgcgag cccggttatt attatttgac
     2281 ccttcgagtc tcagattttg cgattctggt attcattagt ttatgtaaca tttaatttat
     2341 tcaatccttt tcaggattgg gtttgtatta tccttctatt gccgtgcacg cagtacacgg
     2401 ttattaatgt ttgacccttc gggtctcttc ttctgcatcc attgtacgcc ttaagtttat
     2461 attgtattca tttgaaatgt tttatttgta acaccgaagg tgtttaatat acataaccac
     2521 gtatgaaata cgtgggaaaa aaagatgaaa aaaaagcaac tccgaaggag ttgaatatga
     2581 ttaattatca aaatcaaata acctgttctt aagattacta ttccatttct attatatcat
     2641 gtgagaatat attggcattc caaagatatc aaaataaaga atagtaatga tggggaaaat
     2701 aatatagtcc tcccgataat tatccagatc ccttcactcg cctgcactaa ttattttaac
     2761 catcagctaa cggccaggca gacataaccc cgtcagactg tcgctgacag gcaggcaaaa
     2821 gagaaatatc aataccgtcc tattgggcta attgatatat caatttggta taaactattc
     2881 ttataattca aatctctcca taaataaata tctcaatggt agtttttaat taccacaaaa
     2941 tgatgtattc aattaaaaca ttattaaact aaaccggaaa accttctggt aatatccaca
     3001 aatgctgaca cgccaagttg ttctgccctt ttatcaaaaa tggggtcgga gagattgttc
     3061 tcttcgttac gaaaggaact taaggcgttt cttaaagtct tgcgtctttg gttaaacgct
     3121 tttttcacga tgtcaacaaa aagcttttca tcacagttca gcgattttgt atcattgcgc
     3181 cgaagcctga ttaccgccga ttttactttg ggaggggggt aaaaaacgtg ttcgtccacg
     3241 gtaaacaaat attctatggt ataaaatgcc tgaagcagca cgcttaagat gccgtattgt
     3301 tttgagccgg gcggcgaggc aatacgctgg gcaacttcct tttgaaacat accaaccact
     3361 tctggtacca tgtcgcggtt ttcaaatacc ttaaaaagaa tctgtgtgga aatattgtaa
     3421 ggaaaatttc cgataatggc gtaaggctcg tcaaaaatgt tgtttaaatt atagtgcaga
     3481 aagtctccgt ggattatctg gtttttgcgg tcagggtatt tttcaattaa aaaagtgtag
     3541 gcttccctgt caagttctat cagggtggtc tggaaatttt ttgaggccca caggtgttga
     3601 gttaagatgc cagtgccggg gcctatttcc agaacctgcc ggtagttatt atgcagggtg
     3661 agactttcgc atatttgcag ggcgatactt tcgtctttca gaaaatgctg gccaaggaat
     3721 tttttggggt aaacaaaagt catatcaatt gatcttatct cccctcaggt tgcggtaatg
     3781 ccggcgcgcc tccaccacaa aaatgcttcc cgggtaatcg gttaatatgc gttcaaaaaa
     3841 ttccatcgcc ctggtataat ttttaaaaat attttcattc agctcgccca gcttaaagag
     3901 ggcttcgtcg gcaaggatgt cctgctgata aaaattgacg agttttacca acagggagtc
     3961 ggcatcggca taacgtccct gccgcaggcg tatcccggct ttctgaaaca gcacttcatc
     4021 gaacagcgga tggtataagc caagcgtgtt tatggaatca agtgtcttga acgcttcatc
     4081 atatttgttg cggaaaatca gcagctcggc ccgtgaatat agccttaggc cggtggtggt
     4141 gctgtcttca tccatattgt cgctgatgag caacgaaagc tgcatggcgt cgttggcaat
     4201 cagtttcgag gtggctgcct tgagaatatc caactgtgcc ctggcgtaat caaattcacc
     4261 gatatagtaa taaagccggg cgttgcggaa tttggcttca aaaccgacca catcgttttt
     4321 aaacgctttt tcaacctgcg aatagagcag gctggcctcc cacacgtcgc ctgtgagcag
     4381 caaaatgtcg gcgagttcaa ttttgcattg ggcaataagc tcggccgata ctcctgtgta
     4441 ttgaattgct ttttgaagca gttcggcgcc ttgtgcaggt ttgtccatat aaaaagcctg
     4501 caggtgtgcc aaattcttaa tgagtcgtaa ggagtaattg ttgtctttat ttttgctgag
     4561 caggtcgaag tattcttttt ccagcagttc cagctcctga aggctgatgt tgggtgtgct
     4621 ggtagctttc aggtatttgg tgttcatttt ttcaaccagc gccgattgga agtagggcga
     4681 atagatatct ttggtggata agatgtaatc ataacagctt atggcgtcgt cataaaaacc
     4741 gtttgaaacg gctattgctg cgagttcgta agggcgttcg ccttcttcat cgaagcgttt
     4801 gtcgatggct ttggcctgaa cagccgctgc gccaaattct ttttcctgta taaaatacca
     4861 aagcagcagc aagggatagg gcctgatgtc gggttctttt ttgatacgct gaagcagaac
     4921 cgtcctgaaa atggtattgc gggtgttgtc ggcgtcgttg gccagcaggt cctgcagttg
     4981 gctttgaact tcttcctgtg agccgggatt gtttttgaca tactcgaggt actggtcgag
     5041 tgcagcgctg aaatcatttt ttttcaggta tatttccgca agttccaggt taaaaaggta
     5101 ggtgtttctt aagagttttt ttccctgttc gtaggttttt atggcgtagt cgggctggtt
     5161 gcgtaataaa aaagcattgg cagtaatgac gatttgttct ttgtctgaaa tcaacgattt
     5221 gacagtgttt tcaaattgtt tttttgcttt ctcgtgttgt ttttcctgct cgtacacata
     5281 gcccagatct actgacaaag catagttgcc gggatttttt ttaatggctt tgttgatgag
     5341 tttttcggct ttgtcgaact gatttaattc cacgaggcag ttgaggtaat aatcataaat
     5401 gaaaggggtg gaagttttat tatagagttc ttcaaataag acaacggctt tttcaaattc
     5461 tttatcctga aaatactgta tggccagttg ttcgttggtg cctggctggg ctgcaccttg
     5521 ataagcggta gtcagaagaa aaaataccag caccggtaac cggacaaaag accatagcct
     5581 gcatggatgt tttgcttgtt tgtgttggtc aggcatggta ttattttttt tctgccggag
     5641 aatcatcttt tggatatgtc tccaaaagtt tcagcaaata gggatgaacg gtgtcgaagt
     5701 tggcatattg ttgcagcacc tggtttatct gcctgttgat ttggaatgtc agatcgtgca
     5761 cggatttgga ttccatggta aaataatctt taaattcttt ttcttccagc aggcttttgt
     5821 ctacatcgtg tctgaggttt tcaagttgtt cgcgcgagat tttcaattcc tgttgataag
     5881 atttgtgctt gtttaccatc actttaaagg ctttgcgcac ttcctgaaag gcacacatgt
     5941 atttccagtt ctcgtcattg cggtattcgt ggtaatgaac aataacacgc ttgttggttt
     6001 ctttaaaatc attgtatttt tttttcaccg tgtcaatatt cattttttca agattgcttt
     6061 ctgtagagtc aataatagta atgaggctgt cgatttttgc aatctgttgt ttcttttctg
     6121 cgttatggca tgagctcaat gcgagtaaac caatggctac ggcgagaata aatggggtgc
     6181 gcaacatgat agtgctatta gttaataatt tcaaaacctg tatatcgtgt gagtgctgcg
     6241 ggaatcctga tgccgtcggc tgtctggttg ttttccaaca gggcagctac tattcttggt
     6301 aacgctaatg cgcttccgtt tagtgtgtga actaaattta ttttcccatc tttgttttta
     6361 tagcggagct tcatccggtt ggcctgaaaa ctttcaaaat tggaaacgga gcttacttcg
     6421 agccatcttt tttgtgcggt tgaaaaaact tcaaaatcaa aagtcattgc cgaggtgaag
     6481 ctgatgtcgc caccgcaaag tttcaatatc ctgaacggca gttcaagctg tacaagcagg
     6541 tttgccacat agtcgcacat ttcttttaat gtgtcgtacg atttttcggg gtgttgtatc
     6601 tggacaatct ccaccttgtc aaactggtgc aggcggttga gtcccctgac atctttgccg
     6661 taggagccgg cttcgcggcg gaagcaggaa gaatacgcta cgtttttcag gggcaggtcg
     6721 ctttctttca ggatcatatc cctgtaaagg ttggtaacgg gcacttcggc tgtaggtatc
     6781 aggtaaagtt tgtcttccgg agcataatac atttgtccgt ctttgtcggg cagttgtccg
     6841 gtaccataac cggaggcctc gtttaccatc agcgggggct ctatctccag gtagcctgca
     6901 tttacagccg aatcgagaaa aaattgtatc agcgcacgct gcaggcgtgc gcccttgccg
     6961 aggtaaaccg ggaaaccggc accggtaatt ttgttgccga gctcaaagtc gataagtttg
     7021 tatttcgatg ccaactccca gtggggcagg tcgttgccgg gatttaacgc ggggatgtct
     7081 gctccgccgg taaacacaat ttcgttgtct tcagctgatt ttcccggctt gatggagggg
     7141 tggggagtat tgggcaactg aagcaggagg tttttttgtt tttcctcgct ggtctttagg
     7201 ttgtcttcca actctttgac cgttgttttt agctcaatgg atttttcttt taacaggttg
     7261 gcttcctgat gtttaccgtt tttgaagagt tcgccggttt ctttggccat tttgttgagt
     7321 tcggccaaca ggtcgtcgag ttttttttgg gtggctcttc tttcgtcgtc gcaggcgagt
     7381 atgtcggcaa ctaattgcgt ggcgtcaaag ttttttatgg ccagggcttt gacaatattt
     7441 tctgcatcgt gccggaggct gctgagttgt atcatggtaa atattttttt acaaaattag
     7501 ttttctttac ggaaaaaaaa atctccgatt acaaaaagca accggagatt ttcttattaa
     7561 tttgattggt tattgaattt tttcggtgtc ttgtttttcg agtggggtaa cagaaacata
     7621 cgaacggttg tttttctttt tttgaaattc aacaattccg tctgttaatg caaacaatgt
     7681 gtggtctttg ccgatgccga cattttggtc gggattgtga acggttccgc gctgacgaac
     7741 gatgatattt cctgcagtgg ccaactgtcc gccataaatt tttactccaa gacgtttgct
     7801 gtgtgattcg cgaccgttgg tcgaactacc agcacctttt ttatgtgcca taactgttaa
     7861 tttttaattt ggtgttgtaa ctaaacgaga atatcttcaa tctgaatttt ggtcatgtat
     7921 tgtctgtgtc cgttggattt ttgatatcct tttcttcttt tctttttaaa aacaatcact
     7981 ttgtcgcctt tcatgtgcga gaggattttt gcattgatgc ttgctccggt tatggtgggc
     8041 gttccaactt ttaccgcgcc ttcattgtca atgagcatta ctttatcaaa agtaacggcg
     8101 gttccttctt ctccttcgag gcggtgaaca aaaatttcct gttctttttc aactttgaat
     8161 tgttgtccgg ctatatctac tattgcgtac attataaaga ttttatgttt ttaaaaatgg
     8221 actgcaaaga tatacaaaat tttatttcca aacacacaga ccttaatttt ttttcaataa
     8281 attttttaaa ataatttgtg gaagtaaaaa atatgtatac ctttgcactc cctaaaaaac
     8341 gttatacaat gaaaagaaca tttcagccat cgcagcgcaa aagaaagaat acacacggtt
     8401 ttcgcaaaaa aatggaatca gctaacggtc gcagggtttt agcaaaccgc agggctgaag
     8461 gaaggcataa attaaccgtt tcagacgaaa aacaacataa aaagtaattt gctataaaac
     8521 gcagcagttt aataaaaaca tttaattttg aggagataat tgagtttgtc tcctcatttt
     8581 tttttaattc atagctcatg ggcttctttt tctggttctt aataattgtt tttatcggtt
     8641 ggatactttt tttatatgtt ataccgtttt ttgccagact gtatttaaaa aaacttgcca
     8701 aaaaatttga aaaccattcg caacaaaccc aggcccctga aaaaaaagaa ggctccgtca
     8761 atattgatta catccccgaa aaacacctgg agaaagataa ggaagcgccc ggagattatg
     8821 taaattttga agatatagaa gaaaaataat atacttatga aaaattttga ttacaaaaaa
     8881 tttatcccct atgttgttgc aattgttgtg tttattgcga tttcgctgat atacttcagc
     8941 ccgctgctcg aaggcaaaca gttgtaccag agcgatattg tcaattatcg aggctccgca
     9001 caggagataa aagattttcg ccaggccacc ggcgaagagg ctttgtggac caattccatg
     9061 tttggcggta tgccggctta tcagattgat gtttctttta agaccaatat tgccaaagtg
     9121 ttcaataata ttttcaagtt gtggttgcct catcctgcaa gtatattaat ggtattgctc
     9181 atctgttttt ttatcctggt attaacctta aaaaaagatc cctggctggc tatagttggg
     9241 gctgtggctt ttgccttctc gtcttatttt ttccttacta tcagcgtggg acacaatgct
     9301 aaaactctcg ccatcgctta tatgccggct gttttggcgg gtattattac ggcctaccgc
     9361 ggcaaatacc tactgggcgc ggcattaacg gcattgttta tggctataga aattgccgtg
     9421 aaccatgtgc agatgaccta ttacctgatg atgattctgg gatttgttgt gctcttcgaa
     9481 tttatttacg ccatcctgaa gaaaaacctg gccaattttt ggaaagctac cgctgttatt
     9541 gttgtcgccg gatgcatatc gctgattccc aacctctcca acctgtggac ttcttatgaa
     9601 tatatgaagg aaaccattcg gggcaaaccg gagctgactt caaaccagga aatacgttcc
     9661 tccggcctgg acaaggatta tattacccaa tggagctatg gcgtaggcga aacctttacc
     9721 ctgatgatac ccaatgtgaa gggcggcggc gatgccatgc tgggcgaatc caaatcagcg
     9781 ctggataagg tggacagaaa cctccgcaag caaatatcac aacaaaatca ttattggggc
     9841 aatatgcctt tcaccgcagg tcctgtttat gcaggagctt ttgtgtgttt tctctttgtt
     9901 ttgggattgt ttattgtgaa gggaaaactc aaatgggcgt tgtttggagt taccgtgctg
     9961 gctatcatgc tggcctgggg acataatttc atgccactca ctgatttttt cattgattac
    10021 gttccttttt ataacaagtt cagggcagtg tcgtccatac tgattattgc cgaggttact
    10081 atcccgatac tcgccatcct ggccctgaaa gaaattgccg acaacccggg cctgataaaa
    10141 gaaaaaaaga cggctttttt tgtatcatta gggctcacgg ccgggttaac cctgcttttt
    10201 gtactcctgc caaaaatgtt ttttgatttt atcaaagata ccgaggcggt tcaattcgac
    10261 ggatacgttc agcagggagc caaccaggcc cagatagatg attttatcag caatctggag
    10321 atcgcccgta tttccatttt caggatgtcg tgcctgcgca ctatattatt tattgtatta
    10381 ggagccggta tgttgtgggc ttatgcctcg gcaaaaaaga tcagtaaata catcctgtat
    10441 gcaggcgtag gcgtattgat cctcatcgat atggtgcctg tggacaaaaa atacctcaat
    10501 aacgataact tcattgctaa aaataaaaat gaaaatcctt ttgtggaatc ggctgccgat
    10561 caggcaatac tggcagatac ttcggcggct tacagggtgt tgaatcttac cgtcggcaat
    10621 tttaccaccg atgccaccac ctctttttat catcagtcta ttggcggcta tcatgctgcg
    10681 aaacttcgcc gttatcagga ccttatcgaa caccgccttt ttgacgaaca cggacaactg
    10741 gtaaaaacct tacagaaaac accaaccgac tcggctttaa aagcaaccat gatcggcctc
    10801 acctcgctga acatgctcaa taccaggtat tttatttata accccgatgc gcagccttta
    10861 cgaaatcctt atgcaatggg caatgcctgg tttgtcaacg aggtaaaaat ggttgccaat
    10921 gccgatgaag aaatcaaggc gcttaacaac tttgtgccgg cagccactgc catagtggac
    10981 aaacgttttg aaagcgaact tggcggatta aaaattgcca gagattccgg agcagccatc
    11041 cgtctcgtga aatatacacc taaccacctg acctacgaaa catcaaaact cacatcagac
    11101 cagttggctg tattttccga aatatattat aacaaaggct ggaatgctta tatcgacggc
    11161 caggctaaac cctatgtgcg tgccaattat gtgctgcggg ccatggtggt tccggcagga
    11221 tcacacaaaa ttgagtttaa gttcgaaccc caatcatacg ttaccggtga aaaagtgtct
    11281 ttggccggct cggtattgtt gctgctgata attgtggcgg ccttatattt tgaattccgc
    11341 aagaaaaaag gtgcccctgc cgaacagcgc gaaatcccgg atgaagtgcc tgttgcttct
    11401 gtcgcagcc
//
LOCUS       DAOSFP010000018        11408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4792_length_11408_cov_4.877918, whole genome shotgun sequence.
ACCESSION   DAOSFP010000018 DAOSFP010000000
VERSION     DAOSFP010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4792_length_11408_cov_4.877918"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1114
                     /locus_tag="PLI16_01125"
     CDS             80..1114
                     /locus_tag="PLI16_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOH83191.1"
                     /translation="MKKDKIKFAVVGCGHIGKRHAEMIQRNDEAELVALCDIKPKETL
                     GIDQYEVPFFSDIDTMLKSDIGFDVVNVCTPNGWHVDHTFKSLCAMKHVVCEKPMGLT
                     KASVEQVIYKSLQVSRHLFLVMQNRYSPPSVWIKEVVDKKLLGDIFHVQLNCYWNRDE
                     RYYKKGNWKGTADLDGGTLFTQFSHFIDIMYWLFGDITDIQGIFADNNHQCLTDFEDS
                     GIVSFKFIQGGMGCINYSTSVWDTNLESSITIIGSNGSIKIGGQYMNEVEYCHIKNYS
                     MPTLDQVNPANDYGTYKGSAANHHYVIENVVATLKGRTTITTNALEGMKVVDIIERIY
                     EVRNKNFKKQ"
     gene            1131..2426
                     /locus_tag="PLI16_01130"
     CDS             1131..2426
                     /locus_tag="PLI16_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH83192.1"
                     /translation="MNTFEKLIAKQTKLSVIGLGYVGLPIALEFAKKIQVIGFDIKDD
                     RVEMMKNKIDPSEELPSSAFEGCDILFTSKPEDLKDATFHIVAVPTPIDKHQLPDLTP
                     LLAATRTVGKILKKGDYVVYESTVYPGCTEEDCIPILEELSGLKFVEDFKVGFSPERI
                     NPGDKDHTITTIKKIVSGCDDESLDIVAKVYEIVVKAGVHRASCIKVAESAKIIENTQ
                     RDVNIALMNELSIIFNRMGINTFEVLEAAGTKWNFLKFYPGLVGGHCIGVDPYYLTFK
                     AKEYRYHAQIINSGRFVNDSMGFYIAKQTVKKIIAAGKNVLNSKVLVMGVTFKENVSD
                     IRNSRVADVVSELKSYGVLVDVVDPFANSEEVKHEYGFDMAEKAGGNYDAIIVAVSHL
                     EYTMLTEADFKPMAADKCILVDVKGMYRGKIKQMSYWSL"
     gene            2431..3450
                     /gene="galE"
                     /locus_tag="PLI16_01135"
     CDS             2431..3450
                     /gene="galE"
                     /locus_tag="PLI16_01135"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOH83193.1"
                     /translation="MKILITGGTGYIGAHTVVELQQSGHDVLIIDNLSNSYPEVVLSI
                     QKITGKMPVFEKVELCDSAQVDDFFNRHPGIDGVIHFAALKAVGESTKLPLRYYQNNL
                     FSLINLLKSMNKHQVNTLIFSSSCTVYGQPEKLPVDENSPLQPPMSPYGDTKQIAERI
                     ITEQSKVSGLKSILLRYFNPVGAHPSALIGELPIGVPNNLVPFITQTAAGKRDCLNVF
                     GNDYDTPDGTCIRDYIYVVDLAKAHVTALNRILEGKNASNCEVFNLGTGNGFSVLEVI
                     HSFEQVSGKKLNYRFAPRREGDITSIWSDCALANNVLGWKAEKNLDEMMLSAWNWEKQ
                     LGKNN"
     gene            3461..4519
                     /gene="rfbB"
                     /locus_tag="PLI16_01140"
     CDS             3461..4519
                     /gene="rfbB"
                     /locus_tag="PLI16_01140"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOH83194.1"
                     /translation="MFKKNILITGGAGFIGSHVVRLFVNKYPNYRIVNLDKLTYAGNL
                     ENLHDIDQAPNYVFVKGDITDTDCVQSLFQQYAFDGVVHLAAESHVDRSITNPLEFVV
                     TNILGTATLLNAARNAWKENPEGKLFYHISTDEVYGSLGKEGFFLESTAYDPRSPYSA
                     SKASSDHLVRAYHHTFGLPVVVSNCSNNYGPNQFPEKLIPLMINNIIHNKPLPVYGKG
                     ENVRDWLFVVDHARAIDLIFHKGKPGETYNIGGHNEWKNIDLVLLLCKIMDAKLKREP
                     GTSATLITYVKDRAGHDLRYAIDSAKLTNELGWMPSLQFEEGLEKTVDWYLTNESWLK
                     NITSGDYLKYYEQQYHNR"
     gene            4522..5529
                     /locus_tag="PLI16_01145"
     CDS             4522..5529
                     /locus_tag="PLI16_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH83195.1"
                     /translation="MYENTYHTDDISRLRFLVTGGAGFIGSNIVEYLVKHKAGKITIL
                     DNLSTGFLHNIQPFLTQPNVQFIQGDICDAGTCETSCQDIDIVLHQAALGSVPRSIKD
                     PVATNHANVDGFLNIMTAAKNNKAKRFVFASSSSVYGDSQQLPKTEDIIGRPLSPYAV
                     SKYVDELYAQVFARCYNMEIIGLRYFNVFGPNQSPAGAYAAAIPLFIHALITNRSPKI
                     FGDGEQTRDFTFVENAVQANIKAALTHNTEARGEIFNIACGDRTTVNELFQVLKDISG
                     SDIIPVYLDERPGDIRDSLADISKAHQTLGYMPGVKIKDGLELTYKWFSQNFARLYGD
                     I"
     gene            5516..6262
                     /locus_tag="PLI16_01150"
     CDS             5516..6262
                     /locus_tag="PLI16_01150"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOH83196.1"
                     /translation="MAIFDFRHKQKNGPFNYTDFYSTDLHSHLIPGIDDGSPDMDESL
                     KIIAGLSSMGIKKIITTPHISMLYPNTPEKIYEIFTELQREVAARNIEISLEVAAEYM
                     IDENFVEILKNGKLMTFGKNYVLIELSPYNPFPILSDLIFEMQIKGYNIVLAHPERYL
                     YWQYEFSGLEKLKDRELLFQLNILSLTKIYSQDVHKLAVRLIKEGMVDFVGSDTHSMD
                     YIPKMKDVLTGRLFQDLAATGQIKNNLLSD"
     gene            complement(6254..7135)
                     /gene="fabD"
                     /locus_tag="PLI16_01155"
     CDS             complement(6254..7135)
                     /gene="fabD"
                     /locus_tag="PLI16_01155"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOH83197.1"
                     /translation="MKAYVFPGQGAQYVGMGKDLYEKFPEARQMFEQANGILDFRITD
                     LMFAGTDEDLRQTKVTQPSIFLHSVILSVILGDKFMPDMVAGHSLGEFSALVASRALS
                     FEDGLRLVSKRAQAMQKACESAPGTMAAIMGIEDEKVEAILNEIDDVVVAANYNSPGQ
                     LVISGSVSGITLACERIKAAGARRALPLNVGGAFHSPLMEPARADLALAIEQTRFHKP
                     VCPVYQNVTAQPSTDPQIIKENLNKQLTAPVRWSKSVSNMVADGATIFTELGPGTVLQ
                     GLVRKIAPAAEIEEVSI"
     gene            complement(7151..7882)
                     /locus_tag="PLI16_01160"
     CDS             complement(7151..7882)
                     /locus_tag="PLI16_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOH83198.1"
                     /translation="MNMFNEENVSIKYRDPEEIASVTRGFMSKVFSWMFLAMAITAVT
                     SFLWAGSPSLMSVLVKPEGGLSVLGWIVMFAPLGFVLLMSFGYQKLSAPLMTLLFIVY
                     AVLMGMSFSFIFFAFSLGTIGITFAVTAGMFGVMALAGYFTKTDLTKFGSILMMGVIG
                     IVIAMIVNFFLKSSMMGYVISVIGVLIFTGLTAYDVQKLKRIGMGIGVEQTDVRKMAI
                     MGALTLYLDFINLFLFLLRLFGSRE"
     gene            complement(7913..8515)
                     /gene="folE"
                     /locus_tag="PLI16_01165"
     CDS             complement(7913..8515)
                     /gene="folE"
                     /locus_tag="PLI16_01165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOH83199.1"
                     /translation="MDDIPDKVIRENSGITEELSVSYRKVLQLIGEDPDREGLLKTPE
                     RVAKAIQYLTHGYDLNPEEILRSAMFKEDYKQMVIVKDIEIYSLCEHHMLPFVGKAHV
                     AYIPNGYIVGLSKIPRVVDAFARRLQVQERLTSQIKNCIQETLNPLGVAVVIEANHLC
                     MMMRGIQKQNSVTTTSDFTGAFLIEKTRTEFLTLISAKLH"
     gene            complement(8570..8983)
                     /locus_tag="PLI16_01170"
     CDS             complement(8570..8983)
                     /locus_tag="PLI16_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOH83200.1"
                     /translation="MVFLTRRETFNAAHRLFREDFSDEKNLEIFGKCSNPNWHGHNYE
                     LFVTVCGDTDPQTGMIINLKTLSELIRLHIIDKIDHKNMNLEVGFMKGRMASTENLAV
                     AIWEQLSGTVAKHHARLHCVKICETENNYVEYYGE"
     gene            9081..10202
                     /locus_tag="PLI16_01175"
     CDS             9081..10202
                     /locus_tag="PLI16_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83201.1"
                     /translation="MKRITLLLGLLFLLNPYRIFSQTAEDFYAAGVEKYNNGLYVDAL
                     LDFTQAIKEKSSYTDAYIYRAMSFNAIGNYAEALDDYTTAIELDPGNADAYTNRAVLK
                     SNLGQNKEAIEDCQKAIAADSGFFRAYFVRGYCRYLIRDYKNAISDYTQTIILQPTYV
                     NAFYNRGMAYSILASYKTACEDFSKVIEMSPKDYRAYVSRGMVNGKLKKFPEALSDYD
                     SAIAIKPDHANAYYNKAYTQFEMKDFANAEKNYSNAIKIKGDYYDAYYNRGLSRYEQK
                     KYEEAISDYNVILDKKPNDFETYLNRGLCKNQQGNIAGAIEDFSKAIEIEPTYVKAYI
                     NRAISNQTLDRHDEACADWKKAMELGSKTAETNYDQHCK"
     gene            10523..10783
                     /locus_tag="PLI16_01180"
     CDS             10523..10783
                     /locus_tag="PLI16_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH83202.1"
                     /translation="MNKQELIDAIAKESKLSKASSKKALEALTKSVGGAMKKGERVGL
                     VGFGSFYVVKRNARTGRNPQTGAKMTIKAKKVVKFRAGSKLI"
     gene            complement(10856..>11408)
                     /locus_tag="PLI16_01185"
     CDS             complement(10856..>11408)
                     /locus_tag="PLI16_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH83203.1"
                     /translation="KNSVRPSANVFITMIAYGGLLSFIALYGQELGVKNASLYFLILS
                     VGIAIARLTSGKEFDRNGPRRIITVCLVLLIVGFPVLALAKNAVLYFISAIIIGFGNG
                     VIFPTFQSMVNNLADSSHRGAANSTLYTAVDLGIGLGMVMAGLIAQKVSLSAIFWIST
                     LVCVAGILFFRFVVLGFYERRYR"
BASE COUNT         3489 a         2672 c         2343 g         2904 t
ORIGIN      
        1 cagtggatat ctgccgttac cgacagttgt actcagggaa ttcttttatg tcctgctgaa
       61 tgtcaataag attcatctca tgaaaaaaga taaaataaaa tttgcagttg ttggatgcgg
      121 ccatatcggc aaacgtcatg cggaaatgat acaacgcaat gatgaagcag aacttgttgc
      181 cctatgcgat atcaagccca aagaaaccct gggtattgac caatacgaag ttcctttttt
      241 cagcgatatc gacacgatgc tcaagtcgga tatcggtttt gatgtggtaa acgtatgtac
      301 gcccaacggc tggcatgttg accacacgtt caaatccctg tgcgccatga aacatgttgt
      361 ctgcgaaaag cccatggggc ttaccaaggc ttccgttgaa caagtaattt ataaatcgtt
      421 gcaggtttcg cgccatcttt ttctggtgat gcaaaaccgg tattcccccc cgtcagtatg
      481 gatcaaagaa gtagtggata aaaaattatt gggcgacatt tttcatgtgc aactaaactg
      541 ctactggaac cgcgacgaac gttattacaa aaaaggtaac tggaaaggaa ctgccgatct
      601 tgacggaggc acactgttca cacagttttc gcacttcatt gacatcatgt actggctctt
      661 tggcgacatc actgatatac agggaatttt tgccgataat aaccaccagt gtctgacaga
      721 ttttgaagac agcggcatcg tcagttttaa atttatacaa ggcggcatgg gctgcatcaa
      781 ctattccact tcggtgtggg acaccaacct ggaaagcagc atcaccatca ttggcagcaa
      841 cggcagcatc aagattggcg gtcaatacat gaatgaagta gaatactgcc atataaaaaa
      901 ttattccatg cccacgctgg atcaggtaaa tcccgctaat gattacggaa cctacaaagg
      961 cagcgccgcc aaccatcatt atgttatcga aaatgttgtt gccaccctca aaggccgaac
     1021 caccattacc accaatgcac tggaaggcat gaaagtggtg gatatcattg agcgcattta
     1081 tgaagtcagg aacaaaaatt tcaaaaaaca ataataaaaa ccataattaa atgaatactt
     1141 tcgaaaaatt aatagcaaag caaacaaaac tctctgtgat tggcctggga tatgttggtt
     1201 taccaatagc ccttgaattt gcaaaaaaaa ttcaggtcat aggattcgac atcaaggacg
     1261 accgtgtcga gatgatgaaa aacaaaatag atcccagcga agaactcccc tcttcagcct
     1321 ttgagggttg cgatatactg tttacatcaa aaccggagga tcttaaggac gccacgtttc
     1381 atattgttgc cgttcccacg cctattgaca aacatcagct tcccgacctc actccgctgc
     1441 ttgccgccac acgtaccgtt gggaaaatac tgaaaaaagg cgattatgtg gtttacgaat
     1501 ccaccgtgta tccgggttgt accgaagaag actgcatacc catcctggaa gaattatctg
     1561 gattaaaatt cgtggaagat ttcaaggtag gtttctcgcc cgaacgcatc aatcccggcg
     1621 ataaagacca taccattacc accattaaaa aaattgtttc gggttgcgat gatgaatccc
     1681 ttgatattgt tgccaaagtc tatgaaatag tcgtaaaggc aggtgttcac agagcttcct
     1741 gtattaaagt ggccgaatcg gcaaaaatca tcgaaaacac ccagcgcgat gtcaatattg
     1801 ccttgatgaa tgagctttca ataattttca accgtatggg catcaacact tttgaggtac
     1861 tggaagccgc aggcaccaaa tggaactttc tgaaatttta tcccggtctg gtcggcggac
     1921 actgcattgg ggttgaccca tactacctga cctttaaagc caaagaatac aggtatcacg
     1981 cccagatcat caattcggga cgatttgtca acgattccat ggggttttac attgccaaac
     2041 aaacggttaa aaaaatcatt gccgcaggaa aaaacgtgct caactcaaaa gttttggtca
     2101 tgggcgttac cttcaaagaa aacgtcagcg acatcagaaa ttcgcgcgtg gccgatgtgg
     2161 tcagcgaact aaagtcttat ggtgtgctgg ttgatgtggt agatcccttt gccaacagcg
     2221 aagaagtaaa acacgaatac ggatttgaca tggcagaaaa agccggcgga aattatgacg
     2281 ccatcatcgt ggccgtttcg cacctcgaat atactatgct caccgaagca gattttaaac
     2341 ccatggctgc cgataagtgc attctggtgg acgtaaaggg aatgtaccgc ggtaaaatca
     2401 aacaaatgag ttactggagc ctgtaatacc atgaaaattc tcatcactgg aggaaccggg
     2461 tatataggtg cacacaccgt ggtcgagttg caacaaagcg gccacgacgt gcttatcatt
     2521 gataatcttt ctaattccta tcccgaagtg gttctgtcga tacaaaaaat tacgggtaaa
     2581 atgcccgttt tcgaaaaagt tgaactctgt gattctgccc aggtagatga ttttttcaac
     2641 cgccatccag gcatcgacgg ggtgatacat ttcgcagcgc tcaaggccgt gggagaatcg
     2701 accaaactcc cgctcaggta ttatcagaac aatctttttt cgctgatcaa cctgctgaaa
     2761 tcaatgaaca aacaccaggt caacacgttg atcttttcct catcatgcac cgtgtatggc
     2821 cagcctgaaa aacttcctgt ggacgaaaat tccccgctgc agccacccat gtcgccctac
     2881 ggtgacacca aacaaatcgc cgaaagaatc atcacagaac agagtaaggt ctccgggctc
     2941 aaatccatac tgctcaggta tttcaaccct gtaggggctc acccatcagc ccttatcgga
     3001 gaactgccca tcggagtccc taataatctg gtgcctttca tcacccaaac cgcagccgga
     3061 aaacgcgact gcctgaatgt tttcggaaat gattacgaca cccccgacgg aacctgcatc
     3121 cgtgattata tctatgtggt ggatctggca aaagcccatg taacagccct gaacagaatc
     3181 cttgaaggaa aaaatgcctc caattgcgaa gtgtttaatc tgggcaccgg gaatggtttt
     3241 tcggtgctgg aggtgataca ttcttttgaa caggtctccg gaaaaaaact caactaccgc
     3301 tttgctcccc gccgcgaagg tgacattact agtatatggt cggactgtgc actcgccaac
     3361 aacgtgctgg gatggaaagc agaaaaaaat cttgacgaaa tgatgctctc cgcatggaat
     3421 tgggaaaaac aacttggtaa aaacaattaa aactaccgat atgtttaaaa aaaatatttt
     3481 aataacaggc ggcgccggat tcatcggatc tcatgtggta cgtttgtttg tcaataaata
     3541 tccgaactac cgtattgtca acctcgacaa actgacctat gccggcaacc tggagaacct
     3601 ccacgacatt gaccaagcgc ccaattacgt gtttgtaaag ggagatatta ccgacaccga
     3661 ttgtgttcag agcctctttc agcaatatgc cttcgacggg gtagtgcatc ttgccgccga
     3721 atcgcatgtg gacaggtcga taaccaatcc gctggaattt gtcgttacca atatcttagg
     3781 cacagccacg ctgctcaacg cagcacgtaa tgcctggaaa gaaaaccccg aaggcaaact
     3841 gttttatcat atttctacag atgaagtata cggttcctta ggcaaagaag gtttcttcct
     3901 ggaatccacg gcctatgatc cccgcagccc ttattcagca tccaaggcca gctccgacca
     3961 tctggtacgg gcctaccatc atacgtttgg cctgccggta gtggtttcaa actgttctaa
     4021 caattatggc cctaaccagt ttcctgaaaa attaattcct ctgatgataa ataatatcat
     4081 tcacaacaaa ccgcttcctg tctacggaaa gggagaaaat gtcagggact ggctgtttgt
     4141 ggtggatcat gcacgggcca ttgacctgat ttttcataag gggaaacccg gcgaaacata
     4201 caacattggt ggacacaacg agtggaaaaa tattgacctg gtgctgctgc tgtgcaaaat
     4261 aatggatgcc aaactaaaac gcgaacccgg cacatccgca acactgataa cttatgtgaa
     4321 agaccgcgcc ggccatgact tacgctatgc cattgactca gcaaaactga ccaacgagct
     4381 gggatggatg ccgtcgttgc aattcgaaga aggcctcgaa aaaactgtgg actggtacct
     4441 gaccaatgaa tcctggctaa aaaacatcac cagcggagat tatcttaaat attacgaaca
     4501 gcaataccac aaccggtagt tatgtacgaa aacacttacc ataccgacga tatcagccgt
     4561 ttgcgttttc ttgtaaccgg aggagccgga tttatcggct ccaatatcgt ggaatacctt
     4621 gtaaaacata aagccggaaa aattaccata ctcgataatt tatccaccgg ttttctgcac
     4681 aatattcaac cttttctgac acaacccaac gtacagttta ttcagggcga tatctgcgat
     4741 gccggcacct gcgaaacttc ctgccaggat attgacatcg tgttgcacca ggccgcactg
     4801 ggatcggtac cgcggtctat taaagatcct gtagccacca atcacgctaa tgtagatgga
     4861 tttttgaaca tcatgacagc agcaaaaaat aacaaggcaa aacgttttgt ctttgccagt
     4921 tcttcttcgg tttacggcga cagtcagcaa cttcccaaaa cagaagacat cattggcagg
     4981 ccgttatcgc cctatgcggt ttcaaaatat gtggacgaat tatacgccca ggtatttgcc
     5041 cgttgttaca atatggaaat catcggcctt cggtatttca atgtcttcgg gccaaaccaa
     5101 agccctgcag gcgcttatgc agccgccata cccctgttta tccatgctct gattactaac
     5161 cggagtccaa aaattttcgg cgacggtgaa caaaccagag atttcacctt cgtcgaaaat
     5221 gccgtccagg ccaacattaa ggcggcctta acccacaata cagaagcccg aggagaaatc
     5281 ttcaatatag cctgcggcga ccgaaccact gttaatgaac tttttcaggt tctcaaagat
     5341 atttccggtt cggacattat cccggtctac ctcgacgaaa ggcccggcga tatccgcgat
     5401 tcgctggctg acatttccaa ggcccatcaa acactcggat atatgcccgg cgtgaagatc
     5461 aaggatggtc tggaactcac ctataaatgg ttttcgcaaa attttgcccg gctatatggc
     5521 gatatttgac tttcgacata aacaaaaaaa cggccctttc aactatactg atttttatag
     5581 tacagatctt cattcgcatc ttattcccgg tattgacgac ggatctcccg acatggacga
     5641 atctctgaaa atcattgccg gcctaagcag catgggaata aaaaaaatta tcacgacgcc
     5701 tcacatttcc atgctctatc caaatacccc cgaaaaaatc tatgaaatct ttacagaact
     5761 tcaacgggag gtagctgcca ggaatattga aatcagtctg gaagtagctg ccgaatatat
     5821 gattgacgaa aactttgttg aaattttaaa aaacgggaaa ctgatgactt tcggcaaaaa
     5881 ctacgtgctg atagaattga gcccgtacaa ccctttcccg attttgtccg acctgatatt
     5941 cgaaatgcag atcaaaggat acaacatcgt gctggcccat cctgagcgct acctttactg
     6001 gcaatacgag ttttccggtc ttgagaaatt aaaagaccgc gaactgcttt ttcagcttaa
     6061 catcctctcc ctgacaaaaa tctattctca ggacgtgcac aaactggctg tcaggctgat
     6121 aaaagaagga atggttgatt ttgtaggatc agatactcac agtatggatt atataccaaa
     6181 aatgaaagac gtacttaccg gaagattatt ccaggatttg gccgccactg ggcagattaa
     6241 aaataatttg ctatcagatt gatacctctt ctatttctgc cgcaggagca attttgcgca
     6301 ccaggccctg caacacggta ccaggcccca actcggtaaa tatcgtggct ccgtcagcaa
     6361 ccatattact aacacttttt gaccaccgga caggagctgt gagttgtttg ttcaggtttt
     6421 cttttatgat ctgtggatct gtcgagggtt gggccgttac attctgatat accgggcata
     6481 caggcttatg gaagcgtgtt tgttcgatgg ccagcgccag gtcggcacgg gccggttcca
     6541 tcaaaggtga atggaaggcc ccgcccacat tcaacggcag cgctctgcgc gcaccggccg
     6601 ccttgatgcg ttcacatgca agggtgatac cgctgacact gcccgaaatg accaattgac
     6661 cggggctgtt ataattggca gcaaccacca cgtcatctat ttcgttgaga attgcttcca
     6721 ccttttcatc ttcaataccc ataatcgccg ccatagtacc gggagccgat tcacaggctt
     6781 tctgcatggc ctgtgcacgt ttcgacacca ggcgcaatcc gtcttcaaac gacaaggccc
     6841 tggaggcaac cagcgctgaa aactcgccca gggagtggcc ggccaccata tcgggcataa
     6901 atttatcacc cagaattact gaaagaatca ccgaatgaag aaatatagac ggctgtgtca
     6961 cttttgtttg ccgcaaatct tcatcggtgc cggcaaacat cagatcggta atcctgaaat
     7021 caagaatacc gttggcttgt tcaaacattt gccgtgcttc aggaaatttc tcataaagat
     7081 ccttgcccat gcctacatat tgggctccct gaccggggaa gacatatgct ttcataataa
     7141 aattttttaa ctattctctg ctgccaaaca agcggagcaa aaacaagaac aggttgataa
     7201 aatcgaggta taaggtcaaa gcgcccatga tggccatttt tctgacgtcg gtctgttcaa
     7261 ccccgatgcc catgccgata cgttttaatt tttgaacgtc atacgctgta aggcccgtga
     7321 aaatcagcac cccgataaca ctgatgacat aacccatcat gctgcttttc aggaaaaaat
     7381 ttactatcat ggcaattact attcctataa cgcccatcat cagtatggaa ccaaatttcg
     7441 tgaggtcggt tttggtaaaa taccctgcca gcgccatcac gccaaacatt cctgcggtaa
     7501 cggcaaacgt gatgcctatg gttccaaggc taaaagcaaa aaatataaaa ctgaagctca
     7561 tacccatcaa tacggcatat acaatgaaca ggagtgtcat cagcggagca gacaatttct
     7621 gatatccgaa cgacatcagc agcacaaaac ccagcggggc aaacatcact atccagccta
     7681 ataccgagag gccgccctcg ggcttcacca acaccgacat cagcgaaggt gatcctgccc
     7741 ataaaaagga agttacggcg gtaatagcca tcgcaagaaa catccatgaa aagactttcg
     7801 acataaaccc gcgggttaca gaagctattt cttcggggtc gcggtatttg atacttacat
     7861 tttcttcgtt aaacatattc ataaaaatat ttttttgcaa aattaataaa aatcaatgca
     7921 gcttggcgct gattagcgtt aaaaactcgg tacgtgtctt ttcgataaga aaagcccctg
     7981 tgaaatccga ggtggtggtt accgaatttt gtttctgtat gccacgcatc atcatacaga
     8041 ggtggtttgc ctcaataacc acggccacac ccaacgggtt cagggtttcc tgaatacagt
     8101 tttttatctg tgaagttagt cgttcctgta cttgcaatcg gcgggcaaaa gcgtcaacca
     8161 cgcgtggaat cttacttaat cctacgatat aaccattcgg aatatacgca acatgggcct
     8221 ttccgacaaa cggcagcatg tggtgctcgc acaaagaata tatttctata tcctttacaa
     8281 tgaccatttg tttataatct tctttgaaca tggctgagcg cagaatttct tccgggttca
     8341 ggtcatagcc atgagtcagg tattgaatgg ctttggcaac acgttccgga gttttcaaca
     8401 agccttccct gtcggggtct tcaccgatca gctgaagcac tttcctgtag gaaacagaca
     8461 actcttccgt aataccggag ttttcccgga taactttatc aggaatatca tccatttcat
     8521 tttcttttag catttcttta tctattgcat tcatttagat attattactt cattcaccat
     8581 aatattccac ataattattc tctgtttcgc aaattttcac acaatgaagc cttgcatggt
     8641 gtttggcaac agttcccgac aactgttccc agatagccac cgccaggttt tcagttgaag
     8701 ccatcctgcc tttcataaaa ccaacctcga gattcatatt tttatgatcg attttgtcaa
     8761 taatatgcaa tctgataagt tcactcagag ttttgaggtt aataatcatt ccggtctgcg
     8821 gatcggtatc tccgcatacg gtaacaaaaa gctcataatt gtgcccgtgc cagttgggat
     8881 tggagcattt tccgaaaatt tccagatttt tttcatcgga aaaatcttcg cgaaacaacc
     8941 tgtgtgccgc attaaatgtt tctcttcggg ttagaaaaac catcttaaat tttttacaaa
     9001 attacaataa aaacgtgagc ctgcctgttt tattaccaac tattaaatca ggctattgct
     9061 atctttgcaa aaaaataaaa atgaaaagaa ttaccctctt gcttgggttg ctgtttttac
     9121 taaaccccta tcgcattttt tcgcagaccg ctgaagactt ttatgcggca ggagtggaaa
     9181 aatacaacaa tggtctttat gttgatgcgc tgctggattt tacccaggcc ataaaagaaa
     9241 agtcgtccta taccgatgca tatatttaca gggctatgtc tttcaatgcc attggcaatt
     9301 atgccgaagc tctcgacgat tacaccacgg caatagagct tgaccccggc aatgccgatg
     9361 cctataccaa ccgtgctgtg ctcaaaagca accttggaca aaacaaagag gccattgaag
     9421 attgccaaaa ggccatcgcc gccgacagcg gtttttttcg cgcttacttt gttcgcggat
     9481 actgccgata ccttatccga gattataaaa atgccatcag cgactatacc caaaccatca
     9541 tattgcaacc gacatacgtt aatgcttttt acaacagggg gatggcctac agcattctgg
     9601 ccagctataa gacggcctgt gaagatttca gcaaagtgat tgaaatgtcg cctaaagact
     9661 accgcgcata cgtgagcaga gggatggtaa acggcaagct gaaaaaattc ccggaagctc
     9721 tcagcgacta tgacagtgcc atagccatca aacccgatca tgcaaacgct tattacaata
     9781 aggcatacac gcaatttgag atgaaagatt ttgccaatgc agaaaaaaac tattccaatg
     9841 ccattaaaat caagggagat tactatgatg cttactataa ccgcggacta agccgctatg
     9901 agcaaaaaaa atatgaggaa gctatcagtg attataatgt catccttgat aaaaaaccca
     9961 acgactttga aacatatctg aacagagggt tgtgtaaaaa ccaacaggga aacattgccg
    10021 gagcaataga agatttcagc aaggccatag agatcgagcc aacttacgtt aaagcatata
    10081 tcaacagagc catctcaaat caaacacttg acaggcatga tgaggcatgc gccgactgga
    10141 aaaaagctat ggagctgggc agcaaaacag ccgagacaaa ctatgatcaa cattgtaaat
    10201 aaaatctttc agcaaaaaaa tcattcgttg tatcttctga cgagccacag gacatttgta
    10261 atatcatgca gtctatccca gtaatgacgg gactctgaga caataattac tatactgtaa
    10321 aaaaggagga aaaagaatat ccggcaaaaa tgttaaaaat aagataatat tttttctcga
    10381 ttttgtctcc atttttcccc acaaccagcc atggttatta acaatcgcct gatgataaaa
    10441 ttttaaacaa ttattgcaaa tatttgttta ttagattatt tttgcaccaa ttaataataa
    10501 attaacgtta aattttaaag acatgaacaa acaggaatta atcgatgcta ttgcaaaaga
    10561 aagcaaatta tcaaaagcct cttcaaaaaa agctttggaa gccttaacaa aatcagttgg
    10621 cggcgccatg aaaaaaggtg aaagagttgg tttagtaggt ttcggatcat tttatgttgt
    10681 aaaaagaaac gccagaaccg gccgcaaccc gcaaacagga gccaaaatga ccatcaaggc
    10741 caaaaaagtt gtaaaattca gagcaggttc caaacttatc taagacaatt caaacttgta
    10801 aaaaaaatcc cggaaatccc cgggattttt ttttgcatca attacatatt ttgtactatc
    10861 tgtaccggcg ttcgtaaaat cccagcacca caaaacgaaa aaacaatatt ccggcaacac
    10921 ataccagcgt gctaatccag aagattgccg acaaagaaac tttttgtgca ataagaccgg
    10981 ccattaccat ccctagtccg atgccgaggt caaccgctgt gtaaagtgtt gaatttgccg
    11041 cacctcggtg agaagaatcc gccagattat tcaccattga ctgaaaagta ggaaatatca
    11101 ccccgttgcc aaaaccaata ataatagccg aaataaaata cagtacggca tttttggcca
    11161 gggccagcac gggaaatccc acaattagca aaactaaaca cacagtaata atcctgcgag
    11221 gcccgtttct gtcaaattcc ttacccgaag taagccttgc tatggcaatg ccgacagata
    11281 agatcaaaaa atataaggat gcattcttca cacctaattc ctgaccatat aaagcaataa
    11341 aagaaagtag tcctccatag gcaatcatgg taataaacac attagcggaa ggcctcaccg
    11401 agttttta
//
LOCUS       DAOSFP010000019        11335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4835_length_11335_cov_4.683865, whole genome shotgun sequence.
ACCESSION   DAOSFP010000019 DAOSFP010000000
VERSION     DAOSFP010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11335
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4835_length_11335_cov_4.683865"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(511..1233)
                     /locus_tag="PLI16_01190"
     CDS             complement(511..1233)
                     /locus_tag="PLI16_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83204.1"
                     /translation="MKSNTIKIFWILLFSIAMGYLESAVVIYIRELYYPEGFAFPMKL
                     ISEKIMITEFFREAATMLMLLGTAIIAGRNAIERFAYFIMCFGIWDIFYYVFLYLILG
                     WPQSLLTWDVLFFIPLTWIGPVLAPVINSATMIFLAAVILFFKHQNGAVKTGMLVWTL
                     LIVGSLVVIVSYTMDYAGFMSQRFSIIQQMGWGNTEGLMEYALSYIPRTFNWWVFFVG
                     LAMHITAILFIMTKNMKNTAGK"
     gene            complement(1230..1673)
                     /locus_tag="PLI16_01195"
     CDS             complement(1230..1673)
                     /locus_tag="PLI16_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83205.1"
                     /translation="MKINLFKVLLFTFLVSSIITTAAYFLLLILFNDSLTVLLKNAEN
                     FFLISFAEISGILIFSILFMVSYFLPLYHSKKNQIMQKTSTELFWNFMPLVCLIASLF
                     AFFILLISNPSNGIEGEGWINMWLIYILSYSGLIAFVYRIKKPTS"
     gene            complement(1686..4475)
                     /locus_tag="PLI16_01200"
     CDS             complement(1686..4475)
                     /locus_tag="PLI16_01200"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOH83206.1"
                     /translation="MKKNDVLLIISVALYSWLFYHQLPGINMLLFSVTLIVMLLIRNK
                     TLIKDTAWYVAASGSIVSAVCVMLYGTWLAFAANVVSLSIVSALSISRGSSVIMAGIY
                     AVYSYLSSIGFMIVDFIERRMQKNNPKGNKFWIKLLLGIGIFIVIIVFFILYRQSNPL
                     FKDLTDKINLDFISWPWVRFTFLGFLILYGFFYSRNFPGVYRWDVNIPMNLNAVHYLE
                     KGSILFGKKINEHGERRSGVILLALLNVMLLVVNALDIIYLWITKKIPDGMTLSEYLH
                     QGTTTLIISIVFAILIILFYFRGYINFSEKNKTIKWLAFAWLLQNVFMLVSTGYRNLL
                     YVGEYNLTYKRLGIFIWLVLTLIGLLTTLYKLYGKKTNLYLFKANGWAFYLFFVLFAC
                     FNWDAVITKFNIQNSKNIDKNYLVELSSPANIPDLLQLPADERDFICDETFDNGSYYE
                     DSYSKRSYYDKLFYRGNYTAKLHKRLFDFLDNRSNVGWQSWNLGAVKTEKEIYSLFEQ
                     GKITKLLLSEQNISSIKALEKLKNIEFLDISGNQVKDYGALADFDKLYYLDASSNSIY
                     RLDSLPDLPKLNTLNLSNNYISDFTALQKFTGLENLNISGNSERIDLLPVSQIRTLHT
                     LNISQNEIKNIKSIAALKNLKTLYISGLKNESALKNLPVLPQLEEIDLSNNSFKFEDI
                     DLYDKFKEFKSLKTINLSGNNLSNLYLLTSAKNKVINFFFTWETEQDVQPVFSNLERL
                     VVQSNNINNPEPLKYYPGLKSLDISNNTLSSINTLAIMNGLESLNLSNTGITNFDTLK
                     NLVKLRELNISSNYISDLSSVSFANLQQLNASNNQIKSLEPLKKLKAIRELRISGNQI
                     EDLTPLAQLPHLEILDISSNPINDFSVLYSMKHLKELRITGVSLDVYNELRTQLPGTK
                     IIARQIYKQSSSSRM"
     gene            complement(4487..4792)
                     /locus_tag="PLI16_01205"
     CDS             complement(4487..4792)
                     /locus_tag="PLI16_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOH83207.1"
                     /translation="MSEEFDINNLDKVFESRIRLGVMSVLSVNDYADFNTLKSLLKVT
                     DGNLASHISALEKCNFILVNKEFVGKKPRTTYKITEEGRLAFERHLSFLEKLIQLNK"
     gene            complement(4789..5424)
                     /locus_tag="PLI16_01210"
     CDS             complement(4789..5424)
                     /locus_tag="PLI16_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83208.1"
                     /translation="MNQQNEHLKNLSEIRSLMESSTKFLSLSGLAGVFIGIFALCGAV
                     VAYLYLSLDIFSDNYYIHLSSGKSFNFGNVAFFVTDAVIVLLLALAVGMIFTIRKARK
                     KGQAIWNAAVKKFLINLFIPLVAGGLFCLVLLYYHYIPLIAPAMLVFYGISLVSASVF
                     TFSLIRYLGYSEIILGLISCFFPGYGLIFWAVGFGLLHIIYGTVMRFKYER"
     gene            5866..6783
                     /locus_tag="PLI16_01215"
     CDS             5866..6783
                     /locus_tag="PLI16_01215"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOH83209.1"
                     /translation="MKIKIILLSFFLSLLLLGSCGQKKEKFADEISRVTDQPYSPDAV
                     AAEESGQKSAGTVTEYEKSSEQNAPQVATEKIPQKIIKTADISFKVDKYGKARTKILD
                     IIKIHKGYVSSENQTGDEYRTTNVMVIRVDAANFDALVENLLKEAVFVDYKRINAEDV
                     TEEFVDINARLKSKKDAMLQYEIILKKAYTINDILEVQQYIRTLQEEIESLEGRLKYL
                     SNKVELSTINLTFYEQGNILPAQSEGFGYKLKKALSWGWQGLVTFFLAIIYLWPLWLV
                     GLITFFVIRYFVRKARKKRAARLQQQQNK"
     gene            6886..7701
                     /locus_tag="PLI16_01220"
     CDS             6886..7701
                     /locus_tag="PLI16_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH83210.1"
                     /translation="MNSIPETITSLSNKRIKNVALLMRKQSERKAQGLMVVEGEKEIR
                     QALTAGVEADSVFICHEIVTDKALVEQCIAVCPPGNLCFISKEVFAKVAYRDDSGGII
                     FLGKSRYLDLESVKLSANPLVVILETVEKPGNLGAVLRTCDAAGVDLLIICDPETDIY
                     NPNVIRSGIGCVFTQKVIACTSDEAIAWLKKNNISIFAAALQTEEFYHQTDLSISSAL
                     VFGTEATGLTEKWRLAADKIVKIPMLGTIDSLNVSNSVAIMVYEAKRQRGFTV"
     gene            7821..8540
                     /locus_tag="PLI16_01225"
     CDS             7821..8540
                     /locus_tag="PLI16_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83211.1"
                     /translation="MFIARNKRNSLMNLSKRLILFSFVLFFITGTAFAQYDYYSPADT
                     SSKTIIDNFGNSKSLLKHNRLGFSMEMGVGVSGSKYGSGTYTYASPFLTYRVSPKFRL
                     DVGVSYIQGFNNNGINEFYFGKNPACLSVFARGNYLVNDKLLISGAVYKTFDLNQPQT
                     SDLNSQKKSLNNYGIMVGAEYKITENLTIGAQVNFSDRNPAYYPGSYYNQNDFNQGFH
                     HGINQYHGFGGMRPYYGFGGW"
     gene            complement(8565..9140)
                     /locus_tag="PLI16_01230"
     CDS             complement(8565..9140)
                     /locus_tag="PLI16_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOH83212.1"
                     /translation="MKLVFTTNNKHKLQEISHILNNSILEVLSLEDIGFKGDIPETAD
                     TLAGNALQKARYIHERYRVDCFADDTGLEVDCLNGAPGVYSARYAGEGCNFEDNIEKL
                     LKEMNDCNNRKACFKTVVALIVNGKEYLFEGKVCGTILRERRGVEGFGYDPLFQPDGF
                     EKTFAEMSPDEKNVISHRYRAIAALKEFLLK"
     gene            9249..>11335
                     /locus_tag="PLI16_01235"
     CDS             9249..>11335
                     /locus_tag="PLI16_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83213.1"
                     /translation="MTIKKLTAIFLFTWACSVNIFSQQHLSLDSLAYPDRFVSRYMPM
                     LTDKELMKIASLPDLTVPPGHQYVNKTTLPYKVDNTLQPYMIYPTWQTGYECGQSASI
                     CNDFTFEINRLRNLPSTVDTNRYVSHFAWNFLNNGYNYTGASYYDSWEITRMCGVPNV
                     ADYGGQLYYGGEKRWMSGYNLWYKAMHNRLYTGYNIQVDTPEGLMLLKQWLHNHLDGS
                     AVGGLANFYAQYGSPNATLPAGTEEGGKAVKTIWGSSPSHTWTIVGYNDSIRFDYNGD
                     GQYTNDIDINGDAVVDMRDWEIGGLKFMNGYAGASWGNNACCYLMYKALADDITVNGI
                     WNHRVSVQYAKQTYDPLLTMKIQLQHTCRNKIKVTAGLSTNLADTRPQVVLDFPIFNF
                     QGGEYYMRGDSTEDGKTVEFGLDISPLLTYIQPGQNAKYFLQVVEKDPSGLYPGKITS
                     YALIDYTAGVTQIDCPSTNVNLNNNDTTRLSVTRSVAFSKVDITTAALPSATINQPYA
                     LQMDAVNGTSPYHWDFLFDFSEKDTAGVSPAVTATQLTANASGYAVQNLAFDFPFYGE
                     KYNKLFVSPDGYIKFDDQKYTWPFIIDKVLLFKSTKMIAPFLADLVFGSGQGFWYEGN
                     SQYAAFRWNAYVSGQSGSSVNVVVKLFPDGKIEMYYNNINVASTTGWISAVSRGDNNN
                     YQYTNFSNKLNVNT"
BASE COUNT         3409 a         2427 c         2376 g         3123 t
ORIGIN      
        1 tgaacaactt aatttattta atttcaatca aatattaacg catcagtaat gattgaaatt
       61 aaaaatcgtg acaattcttt tgaaaaaacc tactttaatg ataacacatt aatagcctgg
      121 tataaaaatg ataaagaggc agacaagact tctgaggatt tcaaataggt tgaaaagtca
      181 gccataccag ttggagaaaa gttgataaat ctttacgata gtaaataatt actaaagtca
      241 gctcttaata tcattgaccg ggcaactcta gaaataaaaa agttcgagcg atagaagttc
      301 tgaaaaacgt agtatactga gcgtatagga gtatttatcg aacgaacata aacaaagaaa
      361 attgaagttt ctagaggtgt cctatactca acaccatgca aatgttatag acgaagattg
      421 ccgccatcaa ccgtggagtt atacataacc gtcacatcct gcggatgcga acgctcacca
      481 aaaatccttt accagcatct acgctgaagg ttattttcct gcggtgtttt tcatgttttt
      541 tgtcatgata aacaggatag ctgtgatatg catggcaagg ccaacaaaaa acacccacca
      601 gttaaatgtg cgcggaatgt agcttagggc atattccata aggccttcgg tatttcccca
      661 tcccatttgc tgaatgattg aaaacctttg tgacataaag cccgcataat ccatcgtata
      721 ggaaactata actactaaag aacccactat caacagcgtc caaaccagca ttcctgtttt
      781 tacagcgccg ttttggtgtt taaaaaataa tatcacggca gccaaaaata tcatggtagc
      841 agaattgata acaggcgcca acacagggcc tatccatgtt agagggataa aaaacaacac
      901 atcccatgtc aggagacttt gcggccagcc aaggatcagg tataaaaaca cataataaaa
      961 gatatcccat atcccgaaac acataataaa ataagcaaac cgttcgatgg cgttacggcc
     1021 ggcaatgatg gctgttccaa gcagcataag catagtagcg gcttcacgga aaaattcggt
     1081 gatcatgatt ttttcgctga tgagtttcat cggaaaagca aaaccttcag ggtagtatag
     1141 ttcgcggata taaatcacca cggcgctttc gaggtaaccc atagcgatgg aaaaaagtaa
     1201 tatccaaaag atttttatgg tattgctttt catgaggttg gtttttttat gcggtaaaca
     1261 aaagctataa gtcccgaata ggacagaata tagattagcc acatatttat ccatccctcg
     1321 ccctctatac cgttggaagg gttgcttatc aacagtatga aaaaagcaaa cagacttgca
     1381 ataaggcata ccagcggcat gaaattccag aacaactcag tggatgtttt ctgcattatc
     1441 tggtttttct tactatgata caagggtaaa aaataggata ccataaaaag aatactgaaa
     1501 attaagatgc cgcttatctc ggcaaaagaa atcagaaaaa aattttccgc gtttttcagc
     1561 aaaaccgtca ggctgtcgtt aaagagtatc agcagtaaaa aatacgctgc cgttgttatg
     1621 atgcttgaca ccaagaaagt gaaaagcaat actttaaata aattgatttt catggctttg
     1681 tttttttaca ttcttgaaga ggaactttgt ttgtaaatct gtcgcgctat gattttggtg
     1741 ccggggagtt gggttctgag ttcattgtaa acgtcgagcg aaacgccggt tatcctgagt
     1801 tctttgaggt gcttcatgct atacagcact gaaaaatcat taatggggtt gcttgaaata
     1861 tcaaggattt caagatgcgg gagttgtgcc aaaggtgtga ggtcttcaat ctggttgccc
     1921 gaaatgcgga gttccctgat agccttgagc ttttttaacg gttccagcga ttttatctgg
     1981 ttgttcgagg cattcaactg ttggagattg gcgaaggata cagaacttaa atcgctgata
     2041 taatttgagg aaatattcag ttcgcgaagc ttgacaaggt tttttaaagt gtcgaaattt
     2101 gtaatgccgg tattgctcag atttagcgat tctaagccgt tcatgattgc caaggtgttg
     2161 atggacgaaa gtgtgttatt ggaaatgtca agacttttaa ggcccgggta atattttaat
     2221 ggctcggggt tattgatgtt gtttgattgc acaaccaggc gctccagatt agaaaacaca
     2281 ggttggacat cctgctctgt ctcccaggta aagaaaaagt tgatgacctt gtttttggcc
     2341 gaggtaagca ggtagaggtt gctgaggttg ttgcccgaca ggtttatggt tttcagactc
     2401 ttaaactctt taaacttgtc atagaggtcg atatcctcaa atttaaaact attattggaa
     2461 aggtcaattt cttccagttg tggcaacaca ggcagatttt tcagtgcact ctcatttttc
     2521 aggccgctga tataaagagt tttcaggttt tttaatgctg ctatcgattt aatatttttt
     2581 atttcgtttt gtgaaatatt gagggtgtgc agtgtgcgga tttgcgatac gggcaggagg
     2641 tcgatacgtt cggagttgcc agaaatatta aggttctcaa gcccggtaaa tttttgcaat
     2701 gctgtaaaat ccgaaatgta attgttcgaa aggtttagtg tgttcaattt tggtaaatca
     2761 ggcaggctgt caagtctata gatactgttt gacgaagcat cgaggtagta gagcttgtcg
     2821 aaatcagcaa gcgccccgta gtcttttacc tggttgccgg aaatgtcgag aaattcaatg
     2881 tttttaagtt tttcgagggc ttttatgctg ctgatgtttt gttcgctcaa caataatttg
     2941 gttattttcc cctgttcgaa taaggagtaa atttcttttt cggttttaac ggcgcccagg
     3001 ttccaagact gccagcccac gttgctgcgg ttgtccagaa aatcaaacaa gcgtttatgc
     3061 aattttgccg tatagttacc tcggtaaaac agtttatcat aatatgacct ttttgaatac
     3121 gaatcttcat aataagatcc attatcgaaa gtctcatcgc agataaaatc tctttcgtcg
     3181 gcgggcaatt gtaacaggtc gggaatattg gcaggggagc ttaactccac caggtagttt
     3241 ttatcaatgt tttttgaatt ctgaatatta aacttagtaa ttaccgcatc ccagttaaaa
     3301 caggcaaaca gcacaaagaa cagataaaat gcccatccgt tggccttaaa caaatacaga
     3361 ttggttttct tcccatacaa cttataaaga gtggttagca agccaataag ggtgagcacg
     3421 agccatatga agataccaag acgtttataa gtaaggttat actctccgac gtacaacaaa
     3481 ttgcgatatc cggttgatac cagcataaaa acattttgca gcagccatgc aaaagccagc
     3541 catttgatgg ttttgttttt ttcggaaaaa ttgatgtatc cacggaaata aaacagtatg
     3601 atcaggatag caaaaacaat tgaaataatc agtgttgtag tcccctggtg gaggtattcc
     3661 gagagtgtca tcccgtcggg tatctttttg gtaatccaca ggtaaataat gtcaagcgca
     3721 ttgacaacca gcagcatcac gttcagcagc gccaaaagta tcactcctga acggcgttcg
     3781 ccatgttcat tgattttctt tccaaaaagt atgctgccct tttccaggta atgaacagcg
     3841 ttcaggttca tcggaatgtt aacatcccag cggtaaacgc ccggaaagtt cctgctgtaa
     3901 aaaaatccgt ataaaataag gaatccaagg aacgtaaacc gtacccacgg ccacgaaata
     3961 aaatcgagat taattttgtc agtcaggtct ttgaacaatg gatttgactg ccggtaaagg
     4021 ataaagaaca caatgattac aatgaaaata ccgataccca gtagtagttt gatccagaat
     4081 ttattgcctt tcggattatt tttctgcatt cttctttcga taaaatcaac aatcataaat
     4141 cctatggacg ataagtaaga ataaactgca tagatgccag ccatgatcac cgatgagcct
     4201 cggctgatac tcagggcaga aacaatgctc agcgagacca cattggcggc gaaagccagc
     4261 caggttccgt aaagcatcac acacacagcg gaaactattg atccgctggc cgctacatac
     4321 caggccgtgt cttttatcag cgtcttattt ctgatgagca gcatgacaat aagcgtcacc
     4381 gaaaacaaca acatattgat gcccggcaac tggtgataaa acaaccaact gtataacgca
     4441 acagatatta taagaagcac atcgtttttt ttcatggtat gattttttat ttgtttaatt
     4501 gaattaattt ctcgagaaaa gagagatgcc gttcaaacgc caacctgcct tcttcagtaa
     4561 ttttgtatgt agtccgtggt ttttttccca caaactcttt gtttaccaaa ataaaattgc
     4621 atttttccaa tgcgctgatg tggctggcaa gattgccgtc ggtaactttc agcagtgatt
     4681 tcagtgtgtt gaaatctgca tagtcgttta cactcagcac cgacatcacc cccagccgta
     4741 tcctgctctc aaagaccttg tcaaggttgt ttatgtcaaa ttcttcgctc atctctcgta
     4801 tttaaaacgc atcacggttc cataaataat atgcagcagt ccgaatccca ccgcccagaa
     4861 aatcaatccg taacctggaa aaaaacagga aatcaatccg agaataattt cactgtagcc
     4921 caggtatctt atcaaagaaa aagtgaaaac cgaggcgctg accagcgaaa taccgtaaaa
     4981 aacaagcata gcgggggcta tcagtggaat ataatggtag tacaaaagca ccaggcaaaa
     5041 caaccctccc gcaaccaaag gaataaacag gttgataaga aattttttca ctgccgcatt
     5101 ccatattgcc tgtccttttt tgcgagcctt gcggatggtg aatatcatac ccaccgccaa
     5161 cgcaagcaac agaacgatta cagcatcggt aacaaaaaat gccacattcc caaaattgaa
     5221 ggattttccg ctcgacagat ggatataata attgtcgctg aaaatatcga ggctcaggta
     5281 gaggtaggct acgaccgcac cgcacaatgc gaagatgcca ataaaaaccc ctgccagtcc
     5341 gctcagcgaa agaaatttcg tggaagattc catcagggag cgtatctccg aaaggttttt
     5401 taagtgttcg ttttgctggt tcattattgt ttgatttaaa aagtactttg cggcacaaag
     5461 ttaatgcgtg aaaaaagaaa agtcaagaaa aatttttaat aacgagctaa tcagcagata
     5521 tacaggtgaa tatttttacc tatcaagaag ggactgtttc aaaatgttca ttttgtgatt
     5581 ttcaccgctt tcatttcccc tccctaaagg gtggaagttg ctgaaaatca gcactccatt
     5641 cagggatggg gtaatgatga ttttcagcaa aggcttattt tgagacagcc tcatatacca
     5701 gtgtcggggt gactgcgcgc cggcctatgc tgtatgcagt caccccgaca ctacaactaa
     5761 aaaattttca gttacaacaa aaagtattat ttttattatg gaatttttgc ataaaaaata
     5821 tctaatcctc gtttttatta aatcaaaaac caaaaaccta aagccatgaa aatcaaaatc
     5881 atcttattat cgttcttttt aagcctactc cttctgggca gttgtggcca aaaaaaggaa
     5941 aaattcgccg acgaaatttc ccgtgtaacc gatcaaccct attcacctga tgctgttgca
     6001 gcagaagaat cgggacaaaa atcggcggga actgttacgg aatatgaaaa aagttccgaa
     6061 caaaatgcgc cgcaagttgc tacagaaaaa atccctcaga aaatcatcaa aaccgccgac
     6121 atcagtttta aagttgataa atacggcaaa gcccgcacca aaatcctcga cattattaaa
     6181 atacataagg gctatgtgtc atccgaaaac cagaccggcg atgaataccg aaccaccaat
     6241 gtcatggtca tcagggtgga tgctgcaaat tttgatgcgc tcgttgaaaa ccttctcaaa
     6301 gaagccgttt ttgtggatta taaaagaatc aatgcagaag atgttactga agaatttgtt
     6361 gacattaacg cgcggctgaa atccaaaaaa gacgccatgc tccagtacga aatcatacta
     6421 aaaaaagcat acacaatcaa cgatattctc gaagtacagc aatacatccg cactctgcag
     6481 gaagagattg aatcgctcga aggccggctg aaatatctca gcaacaaggt tgaactaagt
     6541 actatcaacc ttacgtttta tgaacaggga aacattctgc cggcacaaag cgaagggttt
     6601 ggctataaac tgaaaaaagc gctttcgtgg ggctggcagg gattggttac atttttcctt
     6661 gcaattattt atctgtggcc cttgtggtta gttggtctga ttactttttt tgtgatcagg
     6721 tattttgtta gaaaagcccg gaaaaaaaga gccgcccgtt tgcaacaaca acaaaataag
     6781 taacaggtta ttgacggggt gactacccta gcaaaagcat acgctcagtc accccgtcaa
     6841 ttatttaccg gtttttttct atttttgcca tacatttcga gcaacatgaa ctcaatcccc
     6901 gaaaccatca ccagcctcag caacaagcgg attaaaaacg tcgcgttgct gatgcgcaag
     6961 caatctgaac gtaaagcgca gggcctgatg gttgtggaag gcgaaaaaga gatccgccag
     7021 gcgctgactg caggtgttga agcagattcg gtttttatat gtcacgaaat agtaacagat
     7081 aaggctcttg tggaacaatg catcgccgta tgtcctcccg ggaatttatg ttttatttct
     7141 aaagaggtct tcgccaaagt agcttaccgc gacgacagcg gagggatcat ttttttaggc
     7201 aaatcccgtt atctcgacct tgaatctgtg aaactttccg ccaacccgct ggttgtaatc
     7261 ctcgaaaccg ttgaaaagcc gggaaacctg ggcgctgtgt tgcgcacctg cgatgccgcc
     7321 ggcgtcgatc tgctgattat ctgcgacccg gaaaccgata tttataatcc aaatgtcata
     7381 cgttcgggca ttggatgtgt ctttacacaa aaagtaattg cctgcacttc cgatgaagcc
     7441 attgcctggc tgaaaaaaaa taatatttcg atttttgctg ctgcacttca aacagaagag
     7501 ttttaccacc aaaccgattt gtctatttcc tccgccctgg ttttcgggac agaagccacc
     7561 ggccttaccg aaaaatggcg ccttgctgcc gacaaaatcg ttaaaattcc tatgttggga
     7621 accatcgact cgttaaacgt atccaattcg gtagctataa tggtttacga ggcaaaacga
     7681 caacggggat ttacagtgta atagtgtcgg ggtgactgcg cgccggcatg cactgaaggc
     7741 agtcaccccg acacttgcac cccgacactt gcatttaaaa aaagatttcg taattttgtt
     7801 acaataattc agacataaaa atgtttatag caagaaacaa aagaaactca ctaatgaacc
     7861 tgtcaaaacg tttaattctt ttttcttttg ttctgttttt cattacagga acggcctttg
     7921 ctcaatatga ttattattct ccggcggaca cctcctcaaa aacgataatt gacaattttg
     7981 ggaacagtaa atccctgcta aagcacaacc gtcttggctt ttcgatggag atgggggtgg
     8041 gtgtgagcgg ctccaaatac ggttccggga catatactta tgcgtcgcca tttttaactt
     8101 atcgcgtgtc gccgaaattc cgcctcgatg tgggggtaag ttatatccag ggattcaaca
     8161 ataacggaat aaacgagttt tattttggca aaaatccagc ctgtctgtca gtctttgccc
     8221 gcggcaatta tttggtcaac gataagttgc tgatttcggg cgctgtttat aaaacttttg
     8281 acctgaacca gccgcaaact tccgatctga acagccagaa aaaatcattg aataattatg
     8341 gcattatggt cggcgctgaa tacaagatca ctgaaaacct gaccatcggt gcacaagtaa
     8401 atttttctga ccgcaatccg gcctattatc ccggctcgta ttataaccag aacgatttta
     8461 atcagggatt tcaccatgga ataaaccaat accatggctt tggaggtatg cgcccttatt
     8521 acggttttgg agggtggtaa ctattttata ggttgtttac ctttttattt cagtaaaaat
     8581 tcttttagcg cggcgatagc cctgtagcgg tggctaatga catttttttc gtcgggcgac
     8641 atctcggcaa aagttttttc aaagccgtcg ggctgaaaca acggatcata gccgaaaccc
     8701 tccactcctc tgcgctcccg gagtatggtg ccgcacacct taccttcaaa aaggtattcc
     8761 ttgccgttca caatcagagc caccactgtt ttaaaacagg ctttccggtt gttgcaatca
     8821 ttcatttctt tcagcagctt ttcaatattg tcttcaaaat tgcagccttc gccggcataa
     8881 cgggcagaat aaactccggg agcgccgtta agacagtcaa cttcaaggcc ggtatcgtcg
     8941 gcaaaacaat ctacccggta tctttcatgg atataacgtg ccttttgcag ggcatttcct
     9001 gcgagggtat ctgccgtttc aggaatatcg cccttaaacc caatatcttc aagggataat
     9061 acttcaagaa ttgaattatt gagtatatgg ctgatttcct gtagcttgtg tttgttgttc
     9121 gtggtaaaaa ccagtttcat aatctgtttt tgcacaaaag tacaattatt taattttact
     9181 tatttgtaac aatcagacaa caaaaagtaa ttttcataca tttgcaataa caataatccg
     9241 gcaattatat gacaataaaa aaactcactg caatattttt gttcacgtgg gcctgttcgg
     9301 taaatatttt ttcacagcaa cacctttcct tagactcttt ggcatatccc gaccgttttg
     9361 tttcgcgcta tatgcctatg cttaccgata aggagctgat gaaaattgcc tcgctgcccg
     9421 acctgacggt gcctccaggc catcaatatg ttaataaaac caccttgcca tataaagtgg
     9481 ataatacact gcaaccgtat atgatttatc ccacctggca aaccggttac gaatgcggcc
     9541 agtcggccag catctgcaat gatttcactt ttgaaatcaa ccgtttgcgc aatttgccgt
     9601 ccaccgtgga tactaatcgt tatgtgtcgc attttgcttg gaattttctg aataacggat
     9661 ataactatac aggcgccagc tattatgatt cgtgggagat aacgcgtatg tgcggtgtgc
     9721 ccaacgtggc tgactatggc ggccagttgt attatggcgg agaaaaacgc tggatgagcg
     9781 gttataatct gtggtacaag gcgatgcata atcgtcttta taccggctat aacatccagg
     9841 tggacacccc cgaagggctg atgctgctga aacaatggtt gcacaaccac ctggacggat
     9901 ctgctgttgg cggactggcc aatttttacg cacaatatgg ttcgccaaac gccaccctgc
     9961 ctgccggaac cgaagaaggc ggcaaggcag taaaaaccat ctggggttcc agtcccagcc
    10021 atacctggac catagtgggc tataacgatt ccatccgctt tgattataac ggcgacggac
    10081 aatacaccaa tgatatagat atcaacggtg atgcggtggt ggatatgcgc gactgggaga
    10141 tcggcgggct gaagtttatg aacggctatg ccggggcttc gtggggcaac aacgcctgtt
    10201 gttatctgat gtataaagcg ctggccgacg acattaccgt caatggaatc tggaatcacc
    10261 gggtatcggt acaatatgca aaacaaactt acgacccgct gctgaccatg aaaatacaac
    10321 ttcagcatac ctgccgcaac aaaatcaaag ttactgccgg actttccacc aacctggcgg
    10381 acacacgccc acaggtggtt ttggattttc ccatcttcaa ttttcaggga ggtgaatatt
    10441 atatgagggg cgactccacc gaagacggaa aaactgtaga atttggcctc gatatcagtc
    10501 ctttgctaac ctacatccag cccgggcaaa atgcaaaata ctttctgcag gtggtcgaaa
    10561 aagacccctc ggggctatac cccggaaaaa tcacgagtta tgcccttatt gactataccg
    10621 ccggagtaac tcagatagac tgcccgtcaa ccaacgtcaa cctcaacaac aacgacacca
    10681 cccgcctgtc ggtaacccgc agcgtggcat tcagcaaggt ggacatcact accgccgccc
    10741 tgccttcagc cactattaat cagccctacg cccttcagat ggacgccgtc aatggcacat
    10801 cgccttatca ctgggatttt ctatttgatt tttcagaaaa agataccgcc ggagtgtccc
    10861 ccgccgttac agccacccag ctaacggcaa acgcatctgg atatgccgtg cagaacctgg
    10921 cttttgattt tccgttttac ggtgagaagt acaacaaatt gtttgtcagt cccgacggct
    10981 atataaaatt tgatgaccag aaatacacct ggccctttat catcgacaaa gtgctcttat
    11041 tcaaaagtac caaaatgatt gcaccatttc tggccgacct ggtttttggt tcgggccagg
    11101 gtttttggta cgaaggcaac agccagtatg cagcttttcg ttggaatgcc tatgtttcgg
    11161 gacaatcggg cagcagcgta aatgtagtgg tgaaactttt tcccgacgga aaaattgaaa
    11221 tgtattacaa caatatcaat gttgcttcca ccacagggtg gatcagtgca gtatcccgcg
    11281 gtgacaacaa caattaccag tacaccaatt tctccaacaa gctgaatgtc aatac
//
LOCUS       DAOSFP010000020        11232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_4889_length_11232_cov_4.508813, whole genome shotgun sequence.
ACCESSION   DAOSFP010000020 DAOSFP010000000
VERSION     DAOSFP010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_11232_cov_4.508813"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PLI16_01240"
     CDS             <1..569
                     /locus_tag="PLI16_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83214.1"
                     /translation="TFATMHKNASVWIDLNNTPMGGQTAIQPALPLHIHLADNNSESW
                     ISIQLVTSNGVMLDLGPHTSNLDTTFAIDATAANWIFVRAIQSDSNYLWSAPVYLTGT
                     LTSFEEARQDKDNLIYPNPATTFLTVMAGNGQHHVAIYDLYGRLHYSGVSLQNSRIDI
                     GMFSAGVYLVKVQTAGKTIVKKVVINPD"
     gene            complement(669..1013)
                     /gene="rplT"
                     /locus_tag="PLI16_01245"
     CDS             complement(669..1013)
                     /gene="rplT"
                     /locus_tag="PLI16_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOH83215.1"
                     /translation="MPRSVNAVASRQRRKKILSRVKGQFGRRKNVWTVAKNAYEKGMC
                     YAYRDRRNKKRDFRGLWINRINAGVRLYGMSYSVFIGKLHKKNIELNRKTLADLAMNN
                     PEAFKAIVDAVK"
     gene            complement(1089..1283)
                     /gene="rpmI"
                     /locus_tag="PLI16_01250"
     CDS             complement(1089..1283)
                     /gene="rpmI"
                     /locus_tag="PLI16_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOH83216.1"
                     /translation="MPKMKSISGAKKRLDFTGTGKIKRKHAYKSHILTKKSTKRKRNL
                     TYATTVEKVDEKRVKQMLAC"
     gene            complement(1309..1875)
                     /gene="infC"
                     /locus_tag="PLI16_01255"
     CDS             complement(1309..1875)
                     /gene="infC"
                     /locus_tag="PLI16_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOH83217.1"
                     /translation="MATNFNAPQGGGYQGRGFVKKEELHRINERITAPVVRVVGENIQ
                     MGIYPIREALEIAAEQGLDLVEISPTATPPVCKVIDYKKFLYEQKRKQKEMKAKAAKV
                     VIKEIRLGPNTDDHDFDFKLKHAIKFLEEGCKVKVDVYFRGRSIVYKEQGEIILLKFA
                     QELVDYGKPDQLPKLEGKRMIMILSPKK"
     gene            complement(1930..3864)
                     /gene="thrS"
                     /locus_tag="PLI16_01260"
     CDS             complement(1930..3864)
                     /gene="thrS"
                     /locus_tag="PLI16_01260"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOH83218.1"
                     /translation="MINITLPDNSVRQYNEGVTGLEIALSISEGLARNVLAAQVNDEV
                     WDATRPIHHDAKVKLFTWNDAEGKAAMWHSSAHLMAEAIEALFPGTKFGIGPDIENGF
                     YYDIDTGDRMITEADFKAIEDKMLEYAKENQPIVRKEISKQGAIDLFTKKGDEYKLEL
                     ISELADGEITLYESGHFTDLCRGPHIPGTGFIKAVKILSVAGAYWRGDINRKQLTRVY
                     GITFPKQKELQDYLFLLEEAKKRDHRKLGKELELFTFSANVGLGLPLWLPKGTALRER
                     LEAFLKKVQKKAGYVQVMTPHIGNVNLYKTSGHFQKYGKDSFRPISTPVEGEEFMLKP
                     MNCPHHCEIYNCKPRSYKDLPFRVAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCT
                     PDQVKQEFVGVISIVMQIFKALDFKEWTAQISLRDPNDKEKYIGSDENWEKAERAILE
                     VVEENNINGVVALGEAAFYGPKLDFMVRDALGRKWQLGTIQVDYNLPERFNLEYIGND
                     NQKHRPVMIHRAPFGSMERFVAVLLEHCAGKFPLWLAPEQVTIIPISDKYENYAKKVS
                     QLLNNYDIRGQIDNRSEKAGKKIRDAELDKIPYMLIVGEKEEAESTISVRKQGQGDLG
                     SFTIEAFAALINDEIKKMIE"
     gene            complement(4046..5725)
                     /locus_tag="PLI16_01265"
     CDS             complement(4046..5725)
                     /locus_tag="PLI16_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83219.1"
                     /translation="MSVLTGILVYYFRWSGSEGQEWKSIIDGDGKGYYAYYDQIVFSK
                     NFGTARPDNDHIIKIGDRSLIKYQCGTALLMAPFFLSSALVSGFPDDPYAEAYQKSVS
                     VAAIFYLLLGFVFLVLLLKKLKIKPPHILISLALTLFSTNLLVYVVAQPDMSHVYSFS
                     LISGFLWASHAYIIKPCRRWLIISAVFLGIIYLIRPVNIMILLFVLFFFDDFKSVRNF
                     IQQHKKQLLVFAVIVSLVMAIQNVLWYIQCRHFFLWSYQNEGFYWLHPEIFNVLFSFR
                     KGLFIYTPILIIVFWSLAVLLKTNKFRFFNTLLFLIFISYIISSWWCWTYFDSFGMRP
                     FVDFYGIFVLLLALLLSNIGQRVRTILLLSCIPLLVLNLLQSYQYNKRILSAEYMNFE
                     SYKYIFFKTSLQYVDCIGGAHDLIPYNKFTQQLLFSTADSQPADEQDFGPGKEFSHAI
                     CINNQDELFHAKKMYAQITFKKWDYRPEGTPQALFVAAVSYQGHPNAFYKVFRLDYLP
                     RATVNKWNEFSYTLILPKIEQPTFELKMYIWNRASETFKLKDLTIKIFKTG"
     gene            complement(5779..9216)
                     /locus_tag="PLI16_01270"
     CDS             complement(5779..9216)
                     /locus_tag="PLI16_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HOH83220.1"
                     /translation="MQKFITHVVFICAIILLTTKTQAQYQPGDTISIQTFTFGSPQDA
                     RFLFPPDTVRVNKILMYYTLKCNPAQNPACGEWDYLTNTYLYEHTGVLDSTLLHAPSY
                     MVNGSSPDSVEFMMQPSWDYAPHFNTQMVHTNTLSFDSAVFNNGVIVTENPFNTIFPE
                     SRSQYLWKASELLAAGLSAGNITGLRFNIQNTGSELKNLTLRIKNTVLDSLTSMSFES
                     TGFVTVYSNNRSFPATGWNTIQFTTPFAWNGTDNLAIDISFDNQIPGITNQVFSENTF
                     LKTGVFSAVSDKSLFFFGNDFVDVPQEAFASIDSAITISLWQYGNPLFQPQNQMVFEG
                     YDSLNNRVINAHIPWGDGTIYWDAGNSSTTSYDRINKAANTQDYEGKWNHWAFTKNVA
                     TGSMKIYLNGTLWHSGTGKTKRMYGIKNFKLGAAAPGNYNYDGYIDEFAVWNTELDSA
                     TISKWMRKDLDSGHPYDSSLQLYYKFDDNAMFTAADSSGNVNHGTLFGLPVYVFNKGS
                     EIERNFSETSLRPAVIFEQGTYESHIDTVITIDSVMKDPMQIVFYNDSLQPLIPMDTL
                     TVWPSYYNHYVFDHLGHAVDSTFVIPDSLICLKQWYYYSEHFEVVNRFELGRYITPYG
                     NGLSLGNGITWIFDLSDYRPLLKDSVHLSAGNWQELMDMRFEFILGTPSRDPLSIKNL
                     WNGNFDYGFADNPIENRLTPKKIIIPSNAVNVRWKSRVTGHGMDTPQNCAEFCPKYHF
                     YKVNGVQQYQQLVWRDDCALNPLYPQGGTWVYSRSNWCPGAEVRTYDFEITPFVTPGD
                     TATLDHDVQPYTHTGGWDYYQIEDQLVTYGPYNFTKDAEVYEIKTPSRNFLYKRMNPI
                     CNNPLITIRNNGSDTLKTLTITYGLEGGTPSTFNWSGSLAPTAKADVQLEHFSWAVNS
                     GTFYASVSQPNGVQDEYPLNDRMTTGYDGTQEFPADIIFEVKTNNYPGENEYTVKDDL
                     GNVLLHKTFSQANATYQDTLHLAQGCYEFRFTDNDDDGLYWWAATGDGSGYCRIRRAN
                     GLLFKAYGSDFGREIYQQFTVGYNLPVDESSTEIGFTVFPNPAEDKFYVDISLPFQQD
                     VNIRITDCMGIPCRQYDLINMADQQLAIDMTGCAAGIYFVSLQTKNAVKTVKLMLQK"
     gene            complement(9389..10852)
                     /locus_tag="PLI16_01275"
     CDS             complement(9389..10852)
                     /locus_tag="PLI16_01275"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HOH83221.1"
                     /translation="MQGQKKYQEKLFLSFQLSDRIPIDNFYRRLKDALDLSFISQQTS
                     QYYGSEGQKSIDPIVFFKLMLIGYLENFNSDRKIIEHASMRMDMLYFIGYDIDERLPW
                     HSTLSRTRQLYGEELFLEVFRKVLSLCIKNGMVSGRRQAVDSALVKANASMDSIIPKE
                     LTIDTANYLKELTENEEEENPLQKDKKQKTSMSNRNWTSPTDPDARISKKKHKPLQLN
                     YAGQISVDTHSHVICGAMADFANKRDAMTLPTLVDQVCENLQKEGIQLEEVLADTNYS
                     SGDALRYLENKAIGGYIPCYGPYKNKREDFIYNKEEDCYVCSQGKKLPFKKYIDRKCN
                     GKNKVYYSSRIDCKDCPQKTKCLGKMKIKYLMDTVDKPYYDQMYERVKTKKGKQMKTL
                     RSSTVEPVLGTLLHFLGMRKVYTKGIKLANKHVLMASTAYNIKKLLKFKGVNYAQSGI
                     KKLEKAHKCLNNQLFDFFLTKITCFLAQLKGQSLFIA"
     gene            complement(11077..11149)
                     /locus_tag="PLI16_01280"
     tRNA            complement(11077..11149)
                     /locus_tag="PLI16_01280"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11114..11116),aa:Lys,seq:ctt)
BASE COUNT         3044 a         2350 c         2345 g         3493 t
ORIGIN      
        1 ggacatttgc caccatgcat aaaaacgcct cggtgtggat agaccttaat aacacgccca
       61 tgggtggtca aacggcgata caaccggctt tgccgcttca tatacacctg gctgacaaca
      121 acagcgagtc gtggatttca atacaattag ttacttctaa tggtgttatg cttgacctcg
      181 gcccacatac gagtaatctg gatactacat ttgctatcga tgccacggca gcaaactgga
      241 tatttgtccg tgctattcag tccgacagta attacctgtg gtcggctccg gtctatctga
      301 caggcaccct gacttcattt gaggaggcca ggcaggataa ggacaaccta atatatccca
      361 atcctgccac aacattttta actgttatgg ctggcaacgg acaacaccac gttgctattt
      421 atgatttgta cggtaggctt cattattctg gagtctctct ccagaacagc aggattgaca
      481 ttgggatgtt ttctgcggga gtttatctgg taaaagtgca gacagccggt aaaaccatag
      541 taaaaaaagt cgttattaat ccggattgat ggcgggatac agccatttat tgatgtgtac
      601 aaaaatcaaa aaaaaggaca aaaaaaaacc ggctcgttgc aaggccggtt ttttttgcga
      661 aaaacttttt actttaccgc atccactatg gccttgaaag cttcgggatt gttcatggca
      721 aggtctgcca aagtttttct gttgagttcg atattctttt tatggagttt tccgataaat
      781 accgaataac tcataccata gagacggaca cctgcattga tacggttgat ccacagtccc
      841 ctgaaatcac gttttttgtt acgcctgtcg cggtatgcat aacacatacc tttttcgtaa
      901 gcgtttttgg caacggtcca aacatttttt cttctaccga attgtccttt gaccctgctt
      961 aagatttttt ttcttctttg tcttgatgct acagcattta ctgatcttgg cataatttta
     1021 aattttttgt ttcagcgttc ttctgtaaag aatctttcat gctgaaaaca agttaataat
     1081 tgatttactt aacaggcaag catttgtttc actctttttt cgtccacttt ttccacggtg
     1141 gtggcatagg taagatttct cttcctcttg gtggattttt tggtcagaat gtgactcttg
     1201 tatgcgtgtt ttcttttaat ttttccggtt cctgtaaaat ccagtctttt ctttgctccg
     1261 gaaattgatt tcatttttgg cattttgtga gattttatgt tgttttaatt atttttttgg
     1321 tgataaaatc ataatcattc gttttccttc aagtttgggc aactgatcgg gttttccata
     1381 atcaaccagt tcctgggcaa atttcaggag gatgatttcg ccttgttctt tatagacgat
     1441 ggatctgcct ctgaaataaa cgtccacttt cactttacag ccttcctcca gaaatttgat
     1501 ggcatgtttg agcttaaaat caaaatcatg atcatcggta ttggggccga gccggatttc
     1561 tttgattaca actttggcgg ctttggcctt catttctttt tgtttgcgct tttgttcgta
     1621 aagaaatttt ttgtaatcaa tcactttaca aaccggcggt gtggctgtcg gggaaatttc
     1681 caccaggtca agaccttgtt ctgctgctat ttccagcgct tcgcgtatcg ggtaaatacc
     1741 catctgaatg ttttcgccta ccactctgac cacgggtgcc gtgattcttt cattgatgcg
     1801 gtgaagctct tctttcttga caaatccccg gccctggtaa cctcctccct gcggggcgtt
     1861 aaaatttgtt gctataactt gtcctccatt ttagttaata cttgggttga ttaataaacc
     1921 gaataatctt tattcaatca ttttctttat ttcatcgtta ataagagcgg caaaagcctc
     1981 aatggtaaaa gatcccagat cgccttgtcc ttgttttctt acagagatag tactttcagc
     2041 ttcttctttc tctcccacta taagcatata aggtattttg tccaactcgg catccctgat
     2101 cttttttcct gccttttcgc tcctgttgtc aatttggccg cgaatatcgt aattatttag
     2161 caattgtgaa actttttttg cataattttc atatttatcg ctgatcggaa tgatggtaac
     2221 ctgctcaggg gcaagccata aggggaattt tccggcgcaa tgttccagca gtacggcaac
     2281 aaaacgctcc atggatccga acggtgcccg gtggatcatc acagggcggt gtttctggtt
     2341 gtcgtttccg atgtattcca ggttgaagcg ttcgggcagg ttataatcaa cctgtatggt
     2401 acctagctgc cacttgcgtc ccagtgcatc gcgtaccatg aaatcgagtt tggggccata
     2461 gaaggcggct tcgcccaggg ctaccacacc attgatgttg ttttcttcca ccacttcgag
     2521 gatggctcgt tcggcttttt cccaattttc gtcgctgccg atgtattttt ctttatcgtt
     2581 ggggtcgcga agcgatatct gcgcagtcca ctctttaaag tcaagggctt taaatatctg
     2641 catcacgatg ctgatcacgc caacaaactc ttgttttacc tggtcgggag tgcagaatat
     2701 atgggcgtca tcctgtgtaa atccgcgtac gcgcgtcagg ccgtgcagct caccgctttg
     2761 ttcgtagcga tatactgttc caaactcggc taccctgaaa ggaaggtctt tgtatgatcg
     2821 gggtttgcag ttgtatattt cgcaatgatg agggcagttc ataggtttca gcatgaactc
     2881 ttcgccttct accggcgtgg agataggcct gaacgaatct tttccgtatt tctggaagtg
     2941 cccggatgtc ttatacaggt ttacgttgcc gatatgcggg gtcattacct gtacataacc
     3001 ggcttttttc tgaacttttt tcagaaaggc ttcgaggcgt tcgcgcaggg cggttccttt
     3061 gggcaaccac aagggcaggc caagccccac gttggccgaa aaagtaaata attccaattc
     3121 cttacccagt ttgcggtggt cgcgtttttt ggcttcttcc agcaagaaca ggtaatcctg
     3181 caattctttt tgcttgggga aggtgatgcc gtaaaccctg gtcagttgtt tgcggttgat
     3241 atctccgcgc cagtatgcac cggccacact caaaattttt acggctttga taaagcctgt
     3301 gccgggaata tgggggccac ggcaaagatc agtaaaatgg ccgctttcgt ataaggtaat
     3361 ctcgccatcg gcaagttcgc tgataagttc cagcttgtat tcgtcgcctt ttttggtgaa
     3421 caaatcgatg gcgccttgtt tagatatctc tttacggact attggctgat tttctttagc
     3481 atattcaagc attttatctt caatggcttt gaaatcagct tcggtaatca tgcggtcgcc
     3541 ggtgtcaata tcgtaataga agccgttttc aatatcgggg cctatgccaa atttggttcc
     3601 gggaaaaaga gcttcgatag cttcagccat caggtgagcc gaggagtgcc acatggcggc
     3661 tttgccttct gcatcattcc aggtgaagag ttttactttg gcatcgtgat gtatggggcg
     3721 ggtagcatcc cacacttcat cgtttacctg tgctgcaagt acattacgag ccagtccttc
     3781 gctgatacta agcgcaatct cgagtcctgt aactccttcg ttatattgtc tgaccgaatt
     3841 atcgggtaaa gtaatattta tcatagtttt gatagttcaa aaagagtttg caaagataca
     3901 aaaaaatcaa atacagaaag aaaatatttt taccggcatt ttcagcctta aatcacttat
     3961 tatcaaacaa ttcagatatt cttgataaac aggtgaaata ttttcctgaa acactcaaca
     4021 ccgtttcggt ttatggcagc acgcgtcagc cggttttaaa aatttttatc gtgaggtctt
     4081 tcagtttaaa agtctctgaa gccctgttcc agatatacat tttcaattcg aaggttggtt
     4141 gttcgatttt agggagtatc agggtgtaag aaaattcgtt ccatttgtta acagtggcgc
     4201 gggggaggta gtcgagcctg aaaactttgt agaaagcatt gggatggccc tgataactca
     4261 cggcagccac aaacaaggcc tgtggcgtgc cttcggggcg ataatcccac ttcttgaatg
     4321 taatttgcgc atacattttt tttgcatgga acaattcgtc ctgattgttg atgcatatgg
     4381 catggctgaa ttctttacca ggcccgaaat cctgttcatc tgcgggctgg ctgtccgccg
     4441 tgctgaagag caactgttgg gtaaacttgt tgtacggaat caggtcgtgt gctccgccaa
     4501 tgcaatcaac atactgcaat gatgttttaa aaaaaatgta tttataactt tcaaaattca
     4561 tgtattcggc acttaaaatt cttttattgt attggtacga ctgtagcaga tttagcacca
     4621 gcaaagggat gcaacttagt aaaagaatgg ttcggacccg ttggccgata ttacttaaca
     4681 acagagccag gagcaacaca aatattccgt agaaatcaac aaaaggacgc ataccaaaac
     4741 tgtcaaagta agtccagcac caccaggatg aaatgatata gctaataaaa atcagaaata
     4801 acaatgtgtt gaaaaacctg aatttatttg tttttaataa aaccgccagg ctccaaaaca
     4861 cgatgatcag tataggtgta tagataaaga gtcccttcct gaaactgaac aagacattaa
     4921 aaatttcggg atgcaaccaa taaaaacctt cgttttgata cgaccagaga aaaaaatgcc
     4981 tgcactgaat ataccataaa acgttctgta tggccatgac gagtgaaaca atgacagcga
     5041 atacaagtaa ttgttttttg tgctgttgta taaaatttct taccgatttg aaatcatcaa
     5101 aaaagaacag gacaaaaagc aggatcatga tattgacagg ccttatgagg tagatgattc
     5161 ccaggaacac tgcactgatg atcagccaac gcctgcaggg ttttataatg taagcatgcg
     5221 aagcccatag aaagcccgaa atcaaagaaa aagagtacac atgcgacata tcgggctgcg
     5281 ccaccacata caccaataag ttcgttgaaa acaacgtcaa agccagtgat atcagtatgt
     5341 gcggcggttt gattttcagt tttttcaata acagaacaag gaaaacgaag cccagcagca
     5401 gatagaaaat cgctgcaacg ctaacagatt tctggtaagc ttcggcatag ggatcatccg
     5461 ggaaaccgct gaccagcgcg gatgacagaa aaaaaggagc catcaacagg gctgtcccgc
     5521 attggtattt aatcagggat ctgtcgccta ttttaatgat atggtcatta tcgggcctgg
     5581 cggttccgaa atttttactg aaaacaatct ggtcataata ggcataatat cccttgccat
     5641 cgccgtcgat gatagatttc cattcctggc cttccgaccc tgaccacctg aaataataaa
     5701 ccagtatgcc ggttaaaaca gacaaaacaa caaaaaagat aaagtatggc ttatttgatc
     5761 gtaacaccaa ttatgtaatt atttctgcag cattagtttt actgttttca ctgcattttt
     5821 tgtttgtaaa cttacaaaat aaattcctgc ggcacagccg gtcatgtcga tggccaactg
     5881 ttgatcagcc atatttataa gatcatactg cctgcatgga atgcccatgc agtctgtaat
     5941 gcggatattc acatcctgct gaaaaggcaa agaaatgtca acatagaatt tatcttctgc
     6001 cgggttggga aaaacagtaa accctatttc cgtgcttgat tcatctactg gcaggttata
     6061 tcctaccgta aactgctgat aaatctcccg gccgaagtcg ctgccatagg ctttgaacag
     6121 caggccgttt gcccttcgaa ttctgcagta tccggagccg tcgccggtag cagcccacca
     6181 gtacaggccg tcatcatcgt tgtcggtaaa gcggaattca tagcacccct gcgccaaatg
     6241 cagcgtatcc tgataagtag cattggcctg ggaaaaagtt ttatgcaaca aaacatttcc
     6301 aagatcatct ttaacagtgt actcattttc acccggataa ttatttgtct ttacttcaaa
     6361 aataatatcc gcaggaaact cttgtgtacc atcataaccg gtggtcatcc tgtcgttcag
     6421 tgggtattca tcctgtacac cattgggttg ggaaacactg gcataaaatg ttccgctgtt
     6481 tacagcccac gaaaaatgtt caagctgtac atcggctttt gctgtcggag ccagacttcc
     6541 cgaccagttg aaagtgcttg gtgtgccacc ctcaaggccg tatgtaatgg tgagcgtttt
     6601 caacgtatca ctgccattgt ttctgatagt aatgagcgga ttgttgcata ttgggttcat
     6661 ccgtttatat aaaaaatttc tggatggtgt ctttatttca tagacctcgg catccttagt
     6721 aaaattatat ggtccgtagg ttaccagctg atcttctatc tggtaatagt cccacccgcc
     6781 ggtatgggta tagggctgaa catcgtggtc gagggtagcc gtatcgccgg gggtgacaaa
     6841 tggggtaatc tcaaaatcat atgtcctgac ttcagcgccc gggcaccagt tcgaacggga
     6901 ataaacccag gtgcccccct gcggatacag tgggttcagg gcacagtcgt cgcgccacac
     6961 aagctgctgg tactgctgaa ctccgttcac tttatagaaa tggtacttag gacaaaactc
     7021 ggcacaattc tgcggagtat ccatgccgtg gccggttaca cgcgatttcc agcggacatt
     7081 cacggcattg gaaggaataa taattttctt tggtgtaagg cggttttcaa taggattgtc
     7141 agcaaatccg taatcaaaat tgccattcca caggtttttg atactcagcg ggtcacggga
     7201 cggcgttcca agaataaatt caaaacgcat atccatcaac tcctgccagt tgcccgcgct
     7261 gagatgcaca gaatccttca gcagggggcg gtaatcgctc aggtcaaaaa tccaggtgat
     7321 accatttccc agggataatc cgttaccgta gggagttatg taacggccca gttcaaagcg
     7381 gttgactact tcaaaatgtt cgctgtaata gtaccattgc ttcagacata tcagggaatc
     7441 aggaattaca aaggtggaat caacggcatg gcctaaatga tcaaatacgt aatggttgta
     7501 atatgaaggc caaacggtga gcgtatccat ggggatcaac ggctgcagtg aatcgttgta
     7561 aaacacaatt tgcataggat ctttcataac ggaatcaatg gtgatgaccg tatcaatatg
     7621 agattcatag gtcccttgct caaagattac tgccggcctt aacgaggtct cgctgaaatt
     7681 acgttcgatc tcgctgccct tgttaaacac ataaacagga agtccgaaaa gtgtcccgtg
     7741 attgacattg ccgctggaat cagccgctgt gaacatagcg ttatcatcga atttgtaata
     7801 taactgaaga ctggaatcat agggatgtcc ggagtcgagg tctttgcgca tccatttact
     7861 tatagtagcc gaatcaagtt cggtattcca aactgcgaac tcatcaatat atccgtcgta
     7921 gttataattt cccggagcgg cagcgccgag tttaaaattc tttataccat acattctctt
     7981 tgttttgcct gttccggaat gccacaacgt gccgttcagg tatattttca tgctgcccgt
     8041 agcgacattt tttgtgaagg cccagtgatt ccattttcct tcgtaatcct gcgtgttggc
     8101 agctttattt atgcggtcgt agcttgttgt gctgctgttt cctgcatccc agtatatggt
     8161 gccgtcgccc cagggaatat gggcattgat cacccggttg ttcagggagt cgtatccttc
     8221 gaacaccatc tgattttgcg gctgaaaaag cgggttaccg tactgccata atgatatggt
     8281 gatggcgctg tcgatagatg cgaatgcttc ttgagggaca tccacaaaat cattgccgaa
     8341 aaaaaacaat gacttgtcgg aaactgccga aaagactccg gttttcagga atgtattttc
     8401 agaaaaaacc tggttggtta ttcccggaat ttggttatcg aaggaaatat caattgccag
     8461 attatccgtt ccattccatg caaaaggagt ggtaaactga atggtattcc agcctgtcgc
     8521 aggaaatgat ctattattgc tgtacaccgt gacaaaccca gtactctcaa agctcatgga
     8581 tgtcagcgaa tcgaggacgg tatttttaat acgtagtgta aggtttttca gttcacttcc
     8641 agtattctga atattaaacc tgagccctgt aatattacct gcggacagac ctgcagcaag
     8701 caactcagaa gctttccaca ggtactggga gcggctttcg ggaaagatag tattaaaggg
     8761 gttttcagta acgatgacgc cattgttgaa cacggcagaa tcgaagctga gcgtattggt
     8821 atgcaccatt tgtgtgttga aatgcggtgc atagtcccat gatggctgca tcataaattc
     8881 aacggaatcg gggctggagc cgtttaccat ataacttggg gcatggagca gtgtggaatc
     8941 aagcacaccg gtatgttcat aaaggtacgt attggtcaga tagtcccatt cgccacatgc
     9001 gggattttgg gccgggttgc atttcagcgt ataatacatt agaatcttgt ttacacggac
     9061 cgtatcggga ggaaagagga atctggcgtc ctgcggagaa ccgaatgtga atgtttgtat
     9121 gcttatggta tcgcccggct ggtattgtgc ctgagttttc gttgtcagca atataatagc
     9181 acatataaaa accacgtgtg ttatgaattt ttgcattacc ggtagtttta tttttaagga
     9241 aataaagata cttattcgga tgattatttg aaatttatcg aaaaattttt tctgttagtc
     9301 gcagactaac ttttattcgg tgcgaagttc ggaagtgtct gttgcacaac tacatttttt
     9361 ttacgtattt tttacttttt atcatttttt atgcaataaa taaagattga ccctttagtt
     9421 gagccaaaaa acaagtgatt ttagtcaaaa agaagtcaaa aagttgatta tttaggcatt
     9481 tgtgagcctt ttcaagtttt tttattccgg attgggcata attcacccct ttaaacttta
     9541 atagtttttt tatgttatat gctgtggaag ccatcaggac atgtttattt gctaatttta
     9601 ttcctttagt ataaaccttt ctcattccta aaaaatgaag taaagttcct aatactggct
     9661 caactgttga tgagcgcagg gttttcattt gttttccttt ttttgtctta actctttcat
     9721 acatctgatc ataatagggt ttatcaacag tatccatcag atatttaatc ttcattttac
     9781 caagacattt tgttttttgt gggcagtctt tacaatcaat tcttgaagaa taataaactt
     9841 tattttttcc gttgcatttc cgatctatat attttttaaa tggaagcttt ttcccttgag
     9901 aacacacata gcaatcttct tctttattat atataaagtc ctctctttta ttcttataag
     9961 gaccataaca aggaatatat cctcctatag ctttgttttc taaatatctc aatgcatcac
    10021 cactactgta atttgtatca gctaaaacct cttctaactg tatgccttct ttttgtaaat
    10081 tttcgcaaac ctgatcaacc agggttggca aagtcattgc atctcgctta ttggcaaaat
    10141 cagccattgc tccacaaatc acatgtgaat gagtatccac acttatctgt cccgcataat
    10201 ttaattgcaa tggcttatgt ttcttttttg aaatgcgtgc atcaggatcc gtgggacttg
    10261 tccaattacg attgctcata ctggtttttt gttttttatc cttttgcaat ggattttctt
    10321 cttcttcatt ctcagttaat tcttttagat aatttgctgt atcaatagta agctcttttg
    10381 gaataatact atccatggat gcattggctt tgacaagtgc actatcaaca gcttggcgac
    10441 gaccacttac cattccattt ttaatacata aggacaatac ttttctgaat acttccaaaa
    10501 acaattcttc tccgtacaac tggcgggttc tgctcaaagt gctatgccaa ggtaaacgtt
    10561 catcaatatc atatccaata aaataaagca tatccattcg catagaagca tgttctatta
    10621 tttttctatc ggaattaaag ttttctaaat aaccaattaa catgagcttg aagaaaacga
    10681 ttggatcaat gcttttttgt ccttcgcttc cataatactg agacgtttgt tgggatatga
    10741 atgataaatc cagagcatct ttcaatcgac gataaaaatt atcgattggt attctatcag
    10801 agagttgaaa actcagaaat agtttttctt gatatttttt ttgtccttgc atagtcaaaa
    10861 ttacaaaaaa atatgaagct ataatattga atatcaatta gatatcaaca ttttattgtt
    10921 aattcaatta gattctaata gttttttgag tgttgaattg cagttgcgca acggacactg
    10981 caaacttcgc acaattgatc aaggtttcgt aatttttcta acaatatggt aaataaaaag
    11041 aggctgcctt catgcttcag gcaacctctt ttgctgtctc ctcgcgggga ctcgaacccc
    11101 ggacccattg attaagagtc aattgctcta ccagctgagc tacgaagaca tgagcggcaa
    11161 aagtacaaaa aataatcaaa aaaaaagggg cattgcccct ttttttaatt atttttgaat
    11221 aatcatctta cg
//
LOCUS       DAOSFP010000021        10857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5102_length_10857_cov_3.654786, whole genome shotgun sequence.
ACCESSION   DAOSFP010000021 DAOSFP010000000
VERSION     DAOSFP010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5102_length_10857_cov_3.654786"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLI16_01285"
     CDS             <1..483
                     /locus_tag="PLI16_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83222.1"
                     /translation="NTRYHGSWGAWTKINMTNAAGNIWTANIPQQMAGDTILYYIHAA
                     DQSPRNVNHPLIGQPDPHRFYINGTATTIENYDLTKAIVFPNPATDFLFVQMQNTGDA
                     ETTVLLTDLTGKQVKYISEKNLDTRMLRLDISSLPAGTYFLSIHSGDFLKNQKIMVIH
                     "
     gene            862..2130
                     /locus_tag="PLI16_01290"
     CDS             862..2130
                     /locus_tag="PLI16_01290"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOH83223.1"
                     /translation="MKKTIFIVLLLWSFSLQAAFDDYFENKTLRIDYFHTGNNVTDIY
                     SIDELIEEPYWGGSKVNLIDIFNYGKYKFLVYDKESNTLIYSYTYNTLFSEWQTTDEA
                     KTTFKSFSETVTMPFPKKPVKCEFYTRNKKGIFEKKFEYAINPKNYFINKERRQAYPK
                     IDILNSGNPAVKVDIVVIPEGYTEKEMDLFIRDAEKFAEYLFNARPYKQNKDKFNIWA
                     IKAPSKESGTDIPGKGIWKNTLVGSSFYTFDTERYLMTSDNKDLRDVASNAPYDQIYI
                     IANSDKYGGGAIYNHYAVSVNKNPYAEYIFTHEFGHSFAGLGDEYYDSETSYNDFYPL
                     DVEPPDPNLTTLVNFDSKWKNLIEKDTPVPTPNDPKNKGKVGVFEGGGYVTKGVYRPA
                     YDCTMKSISVDNFCPVCTRAIEKMIEFYSK"
     gene            2167..3075
                     /locus_tag="PLI16_01295"
     CDS             2167..3075
                     /locus_tag="PLI16_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOH83224.1"
                     /translation="MPITKLEQMFDLLKSKEKKQLVAAYANDSHTIGAVSQAVDMGLI
                     EATLVGDTDTINKVCSEMNINPGKFTIVQEADEIKAAEKAVALINEGKGHILMKGLVS
                     SDKYMRAILNKEKGLMSNPNAVLSHVTVIETPQYPKLLIVADVAIIPLPDLKQKIVMT
                     NYLIKTAHALGIEKPKIAILAATEQMSAGMPACVDAAIIAKMADRGQIKGAIVDGPLA
                     LDVAVDKESCEIKKLNSPIHGDTDCILFPNIESGNVFFKTNTKLAGGELGAVVFGAKV
                     PAVLTSRGDSEKTKLYSIALAACLAK"
     gene            3106..4182
                     /gene="buk"
                     /locus_tag="PLI16_01300"
     CDS             3106..4182
                     /gene="buk"
                     /locus_tag="PLI16_01300"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOH83225.1"
                     /translation="MGKRNILAINPGSTSTKIAVFKSNDLVFTTNIKHPSEEIDKFDR
                     ISDQYEYRKNIIVKELEKAGIALSSIEIIIGRGGLVKPIPSGVYGVNEAMKADLRVGV
                     LGQHASNLGGLIADDLAKMIPGAKAYIADPVVVDELEDVARISGHPEFERISIFHALN
                     QKAIGRMHAQAIGKKYEDLNLIVVHLGGGISVGAHRKGKVIDVNQALDGEGPFSPERS
                     GTLPVGALVKLCFSGKYTQKEILKMITGKGGYVAYLGTNSAYEVEQRVTAGDEKAIFI
                     QKAMAYQIAKEIGAMSVVLESEVDSIVITGGMAYDKNFVGLIKEKVNKISSVDVYPGE
                     DEMRALALNGLMLLNGEIIPAEYK"
     gene            4324..5526
                     /locus_tag="PLI16_01305"
     CDS             4324..5526
                     /locus_tag="PLI16_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83226.1"
                     /translation="MKKNLSCLAIFIAVFVFIFSSCKKDNTDDPPATVKTGYVTGKVI
                     AKNGTTPIPNASIFVDADGEIYITKSTLDGSFTLQAPIGEQVLNIQTGGGNIFRAQYP
                     VSIAENQTTTVPSGTLKLLQADSLAYIHGLYDNIQVLIQDSLGYSVHEIFVSDLSNLS
                     LLETFGAIFLNCGKQGVMDSIKYQNLLTYVQNGGSLYASDWAVEYLTGDGNYKSTGHA
                     HEPYGIKATCPNGLPGGFIADSTLCTTKSGPATMVNNAQIVAQDLIDYLGINTINIEY
                     DLGAWEVIELIDIPWEILIQDNNTYGPLAARIYPAPSPNKSIQRMLDQGWVTICHIPP
                     GNPANAHTITISVNALPAHLAHGDFVGSCESVGYGGVIYYTTFHNEVQHNISNDVQHI
                     LQYFILNM"
     gene            5546..6289
                     /locus_tag="PLI16_01310"
     CDS             5546..6289
                     /locus_tag="PLI16_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83227.1"
                     /translation="MKTNTKLLFIALAASLMMFSACKKSNHDLDIIPTGNGPYSPTTD
                     QFMAGLGTTPGYPTGTEFTLPANILIIGEIRGGIQGKSYQVDKQKYNGPFPVLAEDKA
                     WVDYGTGTYVNLYIKFFNALPTPSSVTIPGGLIFVDSSDLHEHIGHYQKGYIMQSITI
                     PVPALDTAFAHIKAYCLNATLAASNYNAVYYIGPITNNPQLNQITTIMAPKQYPFGEE
                     YNIQQIVWDVTDLGLALSAAQIQYLNSLP"
     gene            6996..7790
                     /locus_tag="PLI16_01315"
     CDS             6996..7790
                     /locus_tag="PLI16_01315"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOH83228.1"
                     /translation="MKQYLELLDHVLQQGTAKSDRTGTGTISVFGYQMRFDLNEGFPL
                     LTTKKLHLRSIIHELLWFLQGDTNIKYLKDNNVSIWDEWANENGDLGPVYGKQWRSWQ
                     TSNGQAIDQISQLIQNIKTNPDSRRLIVSAWNIGEIEKMALPPCHVLFQFYVAEGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMVAQVCQLKPGDFVHTFGDAHLYINHLDQARL
                     QLTREPRHLPVMNINPDVKDIFGFHFDDFELQGYDPHPHIKAAVSV"
     gene            7802..8308
                     /locus_tag="PLI16_01320"
     CDS             7802..8308
                     /locus_tag="PLI16_01320"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH83229.1"
                     /translation="MTENKQISIIVAIAEDYGIGKNNQLLWHISEDLKRFKKITSGHT
                     VVMGKNTYLSLPFRPLPKRKNIIITDDPNETFEGCETVYSIHEAIEKMDEHAENFIMG
                     GASIYRQFFGIAQTLYITKVHTMLEADTFFPVISDKEWKLTEESERQSDPDNGLNYTF
                     LTYVKKEK"
     gene            8305..9141
                     /locus_tag="PLI16_01325"
     CDS             8305..9141
                     /locus_tag="PLI16_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83230.1"
                     /translation="MKKTFFVPMLIVAVFTGLSSCQKDTSGEQDSVQQAIISNTFETV
                     SNDIFKTLIYSVIAVNDSLYHPNDSNNFRLNDPCITCNLNSADTLSWPKTLHMTFPAG
                     GCQCADGVSRAGELTIEIPGPAWPLNAKFNVTFNNYTVASAVVSGFKHINITDTENFS
                     FTDSTNLVSVSVNPSGQWSATHYCQWVQGHTTPANITDDLFIYGGNASYESSIDVAEG
                     ISFQVAIVDALQFANFCYWIGSGKTEIISPGHSITTLTYPDSCLNQAVLCVDNKFQTI
                     TF"
     gene            complement(9158..10414)
                     /locus_tag="PLI16_01330"
     CDS             complement(9158..10414)
                     /locus_tag="PLI16_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOH83231.1"
                     /translation="MKKFLLILCCFLLVKPVNSAYLLIPMDNAQQNHLKAYGVAYWIL
                     QREVEVSWLLNYRGGSFMCKYLDVFKDECNIRGVSSEVISDAQAALVLSEIADPEINM
                     DDVKLVKVPKIAVYSPKNKLPWDDAVTLVLTYAEIPYDVVYDEEVLQGLLPTYDWLHM
                     HHEDFTGQYGKFWANYKDAAWYKEDKRLSEETAKKLGYDKVSQLKLAVAKKIRDFVAG
                     GGYLFSMCSAPDSYDIALAAEGTDICDVMFDGDPRDAQAQQKLDYSKCFAFTGFTLVT
                     NPYEYEISNIDVSYSRRVLPQNDFFTLFEYSAKWDPVPTMLCQDHEKIIKAFMGQSTA
                     FNKKFIKSGVLIMGENKSADEARYIHGEYGKGMWTFLGGHDPEDYQHFVYDPPTKLEL
                     YPNSPGYRLILNNVLFPAAKKKKQKT"
     gene            complement(10511..>10857)
                     /locus_tag="PLI16_01335"
     CDS             complement(10511..>10857)
                     /locus_tag="PLI16_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOH83232.1"
                     /translation="DLRESGAIEQDADLVLFIYRPEYYKIEEFEDNTPTAGLAEIIVA
                     KHRNGQVGEVKLRFQKNIGRFTDYEDFKDVEEPAMQPNEAFYEPATKIVQSKINKKSA
                     KKTDDPPVDVPF"
BASE COUNT         3300 a         2638 c         2161 g         2758 t
ORIGIN      
        1 aatacaaggt atcacggatc ctggggggcc tggacaaaaa tcaatatgac caatgccgcc
       61 ggaaatatct ggaccgcaaa cataccgcaa caaatggccg gcgacaccat tttatattat
      121 atccacgcag ccgaccaatc gccgcgaaat gtgaatcacc ctttaatcgg ccagcccgac
      181 ccgcacagat tttatatcaa tggaactgcc accaccatcg aaaactacga cctgacaaaa
      241 gccatagtat tccccaaccc ggccactgat ttcctttttg tgcagatgca aaacaccgga
      301 gatgccgaaa caaccgtttt gcttactgat ctgacgggaa aacaggtaaa atatatctca
      361 gaaaaaaacc tcgatacaag gatgctgcgc cttgacatca gctcgttgcc tgcaggcaca
      421 tattttctaa gcattcattc aggagatttt ttgaaaaacc agaaaattat ggtcattcat
      481 taaccggaaa aattcttaca aatttttcaa tggtaatctg cgactagcaa aacagacacc
      541 cgggcacctc taaaaacttc atcaaaagcc cggccgataa accgtctcta cccttcgcag
      601 gtaggtgctt gattcccaag tgctaatgca ctttgaacta gtacagatac ggtatcgtta
      661 taacctgaaa attttgaaga gttgacccaa agaattttcg cttaaaatca gacataatta
      721 atacttaaaa acctgttttt gttttagttg gctttttgtc aggatatcag cagcaaaatt
      781 gatgaaaacc aaaaattcta atgaatacat tccgtatctc aaaaaaatac tattttcacg
      841 gataatttta aaaattcaac tatgaaaaaa accattttca tcgttttgtt gttgtggagt
      901 tttagtctcc aggccgcttt cgacgactat tttgaaaaca aaaccctgcg tattgattat
      961 ttccataccg ggaacaatgt gacagacatc tactccatcg acgaactgat agaagaacct
     1021 tactggggag gctccaaagt caacctgatc gacattttca attacggaaa gtataaattt
     1081 ctggtgtacg ataaagaaag taatacgcta atatactcct acacctataa cacattgttt
     1141 tctgaatggc aaaccaccga tgaagcaaaa accacattca aatcattttc cgaaacggta
     1201 accatgccct ttccgaaaaa gcccgtcaaa tgcgaattct atacaaggaa caaaaaaggc
     1261 atcttcgaaa aaaaatttga atacgccatc aatccgaaaa actattttat caacaaggaa
     1321 cggcggcagg cctaccccaa aatagacatt ctcaactccg gcaaccctgc agttaaggtt
     1381 gatattgttg taatacccga aggatacacc gaaaaagaaa tggacctgtt tatccgcgat
     1441 gccgaaaaat ttgcagaata cctgtttaat gcccggcctt ataagcaaaa caaagacaaa
     1501 ttcaacatct gggctatcaa agcgccatcc aaagaatcgg gtaccgacat cccgggcaaa
     1561 ggtatctgga aaaacaccct ggtcggctca agcttttata ccttcgacac ggaaagatac
     1621 ctgatgactt ccgacaacaa agacctgcgc gatgtggctt ccaacgcacc ctacgaccag
     1681 atatatatta ttgccaactc cgataaatac ggcggcggtg ccatatacaa tcattacgcc
     1741 gtcagcgtaa acaaaaaccc ttacgctgaa tatatcttca cccacgagtt tggccattca
     1801 tttgccggcc tcggcgacga atactacgat tccgagacct cgtacaacga tttttatcct
     1861 cttgatgtgg aaccccccga tcccaacctg accaccctgg taaattttga ttccaaatgg
     1921 aaaaatctca tcgaaaaaga cacccccgta cctaccccca acgatccaaa aaacaaaggc
     1981 aaagtgggcg tttttgaagg cggcggctat gtaaccaagg gagtgtatcg cccggcgtac
     2041 gactgcacca tgaaatcaat ttcggtggac aatttctgcc cggtgtgtac acgtgccatt
     2101 gaaaaaatga tagaattcta ttcaaaataa atttataaat caataaaaac ctaaaggaga
     2161 ataattatgc ctattacaaa actggaacaa atgttcgatc tgctgaaaag caaagaaaaa
     2221 aaacaactag tagctgccta tgccaacgat tcacatacca tcggagccgt cagtcaggct
     2281 gtagatatgg gactcatcga agccaccctg gttggagata ccgacactat taataaagtg
     2341 tgcagcgaga tgaacatcaa ccccggtaaa ttcaccatcg ttcaggaagc cgacgaaata
     2401 aaagccgcag aaaaagccgt tgcactcatc aacgaaggca aaggacatat cctgatgaaa
     2461 ggcctggtga gctcggataa atacatgcgg gctatcctga ataaggaaaa aggcctgatg
     2521 tcaaatccca atgccgtact cagccatgtt accgtaatag aaaccccgca gtatcccaaa
     2581 ttgcttatcg tggccgatgt agctatcatc ccgctgcccg acctgaaaca aaaaatagtg
     2641 atgaccaatt acctcatcaa aacggcacat gcactgggta ttgaaaaacc caaaatcgcc
     2701 atcctcgccg ccaccgaaca aatgtcggcc ggtatgcctg cctgtgtgga tgccgccatc
     2761 atagccaaaa tggccgaccg cggacaaatc aaaggcgcca tcgtggacgg gccgctggcc
     2821 ctggacgtag ccgttgacaa agaaagctgc gaaataaaaa aactcaacag ccccattcat
     2881 ggcgataccg actgcatact tttcccgaac atagaatccg gcaatgtgtt cttcaaaacc
     2941 aacaccaaac tggccggcgg cgaactcgga gccgtagtgt tcggcgctaa agtgcctgcc
     3001 gtacttacct cgcgtggcga cagcgaaaaa accaaactgt actccatagc actggcagcc
     3061 tgcctggcaa aatagttatt atttttttac agaaatttat ataaaatggg aaaaagaaac
     3121 atcctcgcta tcaaccccgg ctccacctcc accaaaatag ccgtttttaa atccaacgac
     3181 cttgtattca ccaccaatat caaacatccg tcagaagaaa tcgacaaatt cgacagaatt
     3241 tccgaccagt atgaataccg aaaaaacatc atcgtaaaag aattggaaaa agccggtatc
     3301 gccttgtctt ccatcgaaat aatcatcggc aggggcggat tagtaaaacc aataccttcg
     3361 ggtgtttatg gagtgaacga agccatgaaa gccgacctca gggtgggtgt gctgggacaa
     3421 catgccagca atcttggcgg cctcattgct gatgacctgg caaagatgat tccgggcgcc
     3481 aaagcgtata ttgccgaccc ggtagtggtt gatgaactgg aagatgttgc cagaattagc
     3541 ggccaccccg aatttgaacg catttctata tttcatgcgc tgaaccaaaa agccatcgga
     3601 cgtatgcacg ctcaggccat cggtaaaaaa tacgaagacc tgaacctgat tgtggtacat
     3661 ctgggtggcg gtatcagcgt aggcgctcac cgcaaaggca aggtaataga tgtaaatcag
     3721 gccctcgacg gcgaagggcc tttttcgccg gaacgcagcg gcacattgcc tgtaggagca
     3781 ctggtaaaac tatgcttcag cggaaaatat acccaaaaag aaatactgaa aatgatcacc
     3841 ggcaaagggg gctatgtcgc ctacctcgga actaacagcg cctatgaagt agagcaaagg
     3901 gtaacggccg gagatgaaaa agccattttt atccaaaaag ctatggccta ccagatagcc
     3961 aaagaaatag gtgccatgtc ggtagtgctt gaatcggaag tggactctat tgtcatcacc
     4021 ggaggcatgg cttacgacaa aaatttcgtc ggcctgatca aagaaaaagt taataaaatt
     4081 tcttctgtag atgtatatcc cggcgaagat gaaatgcgcg ccctcgccct gaacggactt
     4141 atgctgctaa atggagaaat aatacccgca gaatacaaat aaacaaatac gaactgcggg
     4201 gcttgatgaa aactttttca tgataaaaaa gggatttctg ttgacttccc tggatttatt
     4261 ttgtatattt acaaagcttt tttgaaataa aacgttcttg tttatataca aatccacatt
     4321 cttatgaaaa aaaatctatc ctgcctcgca atttttattg ccgtttttgt tttcattttt
     4381 tcttcctgta aaaaagacaa taccgacgat ccgccggcta cggtcaaaac aggctatgta
     4441 accggaaaag tgattgccaa aaatggcacg actcccatcc ccaatgcctc gatttttgtt
     4501 gatgccgacg gagaaattta cattactaaa tcgacgctgg acggcagctt taccctgcaa
     4561 gcccccatcg gtgaacaagt gttaaacatc caaaccggag gaggaaacat tttcagagca
     4621 cagtaccctg tcagcattgc tgaaaaccaa accaccaccg tgccttcagg aacactcaag
     4681 ctgttgcagg ctgacagtct tgcctatatc cacggcttat acgacaacat ccaggttctc
     4741 atccaggatt cattaggata cagtgtgcac gaaatttttg tcagcgacct gagtaacctg
     4801 tcccttcttg aaaccttcgg cgctatcttt ctgaattgcg gcaagcaggg tgtcatggat
     4861 tcaatcaaat accaaaacct gctcacctat gttcaaaacg gcggcagcct gtatgcatcc
     4921 gactgggctg tggaatacct caccggagac ggcaattata aaagtaccgg ccatgcacac
     4981 gaaccttacg gaataaaagc tacctgccct aacgggttgc ctggcggatt tatcgcagac
     5041 agcaccttat gcacaactaa gtcaggccca gccaccatgg taaacaatgc gcaaattgtc
     5101 gcacaagacc ttatcgacta ccttggaatc aacaccatta atattgaata tgatctcgga
     5161 gcctgggaag ttattgagct tattgacatc ccctgggaaa ttctgataca ggataataat
     5221 acatacggac ctttagccgc ccgcatctat cctgctcctt ctcccaataa atccatacaa
     5281 cggatgctcg accagggatg ggttactatt tgccacatcc caccgggaaa ccctgctaat
     5341 gcacacacca taacgatttc ggtcaatgca ctgcccgcac atcttgccca tggtgacttc
     5401 gtcggatcat gcgaaagcgt gggctacggc ggcgttatat attacaccac cttccacaac
     5461 gaagtccaac acaatatcag caacgatgtt cagcacattt tacagtattt tattttaaat
     5521 atgtaatcag caaaaaattt atcccatgaa aacaaacacc aaacttttat ttattgccct
     5581 ggccgcatcg ttaatgatgt tcagcgcctg caaaaaaagc aaccatgacc tggatatcat
     5641 ccccactggc aatggccctt acagccccac caccgaccaa tttatggcag ggctgggtac
     5701 cacgccggga tatcccaccg gcaccgagtt tacgctgcct gccaatattc tcattatcgg
     5761 cgaaatcagg ggcggcatac agggtaaaag ctaccaggtt gataaacaga aatacaacgg
     5821 acctttccct gtattggctg aggacaaagc ttgggttgat tacggaaccg gcacctatgt
     5881 aaatttatac attaagtttt tcaatgcctt gcccaccccg tcttctgtta ccatcccggg
     5941 aggattgatt tttgtggatt cgtccgacct gcatgaacat attggccatt atcagaaagg
     6001 atatatcatg caatctatca ccattcccgt acctgcttta gatacggcat ttgctcatat
     6061 caaagcttac tgcctgaacg ccaccctcgc ggcaagcaat tacaatgccg tatattatat
     6121 tgggcccatc accaacaacc cgcaactaaa ccagatcacc accatcatgg cccccaaaca
     6181 atatcccttt ggagaagaat acaatattca acagattgtc tgggatgtta ccgacctcgg
     6241 attagcactt tctgccgcgc agatacaata cctgaattct ttgccataat tttgttttga
     6301 ttgttgttat aaaaaaccgg cggtaaatac ctgccggttt tttttatcca cattttgaaa
     6361 actatttcat taaaaaaact atgtttgctc ctccttttaa taaagagttt tcataattac
     6421 tggccgatta attgtttgca catagcacaa atgcatgcta ttagcggcgc gttggcctgg
     6481 tcaaaacggc ggccggcgcc ggggtgaatc aacaggacgg tgaaaatcag caaaggcagc
     6541 ccgcctaaac gggctgtttg tctgaaatgg cctgcaccat ccgctaataa acagatacaa
     6601 ctttttcctt cctttcaaac caggcaaact tttttgtcct ttaatggcca tttcgggcac
     6661 tcctttgcat gcttttcccg gcctgatgag agcgggcgga agcatacgtt ttgacaaggt
     6721 tttttggtaa ctttttttcc gaaaaaaaag ttacattaat aagtacttgc cctgaacggc
     6781 aacacacgag ccttagtagt ggtaacaata aacaacctat caataactgc caaagttttt
     6841 ctgttttttt atctgaatta cgaaagtaga gttatttaca aaaaaataat cttttgcaac
     6901 tcctcaaaaa ttgattggcc gggttttttt atccacattt tgaaaactat ttcattaaaa
     6961 aaaataactt tgtttttttt atcaaccaat tttcaatgaa acaatactta gaacttctcg
     7021 atcatgttct gcaacaggga accgcaaaat ccgaccggac aggcacaggc accatcagcg
     7081 tatttggtta tcagatgcga ttcgacctga atgaagggtt ccctttattg actaccaaaa
     7141 agttacatct gcggtcaata atacatgaat tactctggtt tcttcaaggc gacaccaata
     7201 tcaaatacct gaaagataac aacgtttcca tctgggatga atgggcaaac gaaaacggcg
     7261 acctggggcc ggtatacggg aaacaatggc gttcgtggca aacttcaaat ggccaggcca
     7321 tcgaccagat ttcacaattg atacaaaaca taaaaaccaa tcccgattca cgcaggctta
     7381 tcgtcagcgc atggaacata ggcgaaatag aaaaaatggc gcttccaccc tgccatgtat
     7441 tgtttcagtt ttatgtagcc gaaggaaaac tatcctgtca gttataccag cgtagtgcag
     7501 atatctttct gggtgttccc ttcaatattg cctcctatgc cctgctcacc atgatggtgg
     7561 cacaggtttg tcaactgaaa ccgggcgact ttgtgcacac tttcggtgat gcgcacttgt
     7621 atatcaacca cctcgaccag gccaggctgc aacttacacg ggagccgcgc cacctgcccg
     7681 tgatgaatat caatcctgac gtgaaagata tttttggttt tcatttcgat gatttcgaac
     7741 tgcagggcta cgacccgcat ccccacatca aagcagccgt atccgtttaa tttatactaa
     7801 gatgacagag aacaaacaaa tatccatcat tgtcgccatc gccgaagatt atggaatagg
     7861 caaaaacaat caattgttat ggcacatctc cgaagacctg aaacgtttca aaaaaatcac
     7921 ctcaggacac accgtggtaa tgggcaaaaa cacataccta tccctgcctt tcaggcctct
     7981 gcccaaaaga aagaacatta tcattaccga tgacccaaac gaaacctttg aaggctgcga
     8041 aaccgtttat tctatacatg aagccataga aaaaatggac gagcatgctg agaacttcat
     8101 tatgggcgga gccagcattt acaggcagtt tttcgggata gcccaaaccc tctacatcac
     8161 caaggtacat acaatgctgg aagccgatac ttttttcccg gttatttctg acaaagaatg
     8221 gaaacttact gaagaaagcg aaagacaatc cgaccccgac aacggcctga attatacatt
     8281 tttaacttat gtaaaaaaag aaaaatgaaa aagacatttt tcgtgccgat gttaattgtt
     8341 gcagttttca ccggattgag ttcatgtcaa aaagatactt ccggcgaaca ggatagtgtg
     8401 cagcaagcca tcatttcaaa tacttttgaa accgtttcca atgacatatt caaaacgctt
     8461 atttatagtg taatcgcagt gaacgacagc ctgtatcacc ccaatgattc aaacaatttt
     8521 aggcttaatg acccctgtat tacctgtaat ctgaattcag ccgatacttt gtcatggcca
     8581 aaaaccctgc atatgacatt tccggcggga ggatgccaat gtgccgacgg tgtcagccgt
     8641 gcaggcgaac tgacaattga aataccgggc cctgcatggc ctctcaatgc caaatttaat
     8701 gttacgttca acaattacac ggtggcatca gccgttgtca gtggtttcaa gcacatcaac
     8761 ataacggata cggaaaattt ttcgttcacg gattccacaa atttagtatc tgtctcagta
     8821 aatccttccg ggcagtggtc ggcaacgcat tactgccaat gggtacaggg ccataccact
     8881 cctgccaata ttacagatga cctttttatt tatggcggaa acgcctccta tgaatccagc
     8941 attgatgttg ctgagggaat ttccttccag gttgccatag tggacgcact gcaatttgcc
     9001 aatttttgct attggatagg aagcggaaaa actgaaataa tttcacccgg acattccatc
     9061 accacgctca cctatcctga cagttgtctt aatcaggcgg tgctttgtgt ggataataaa
     9121 tttcagacca tcacttttta acccgaaacc tgctttgtca ggtcttctgc tttttctttt
     9181 tggcggcagg gaataacaca ttattcaaaa tcaggcgata cccgggcgaa ttgggataaa
     9241 gttccagttt ggtaggcggg tcataaacaa aatgctggta atcttcggga tcgtggccgc
     9301 ccaaaaatgt ccacatgcct ttgccgtatt cgccatgaat gtatcgcgcc tcgtcggccg
     9361 acttgttttc gcccattatc aacactcctg attttataaa ttttttatta aaagcagtac
     9421 tttgtcccat aaaggctttg atgatttttt catggtcctg gcacaacata gtaggcacag
     9481 gatcccattt agcagaatat tcgaacaagg taaaaaaatc attctgagga agcacccgcc
     9541 gcgagtacga aacgtcgata ttagatattt cgtattcgta tggattagta acaagggtaa
     9601 aaccagtaaa agcaaaacac tttgaatagt cgagtttctg ctgtgcctgt gcgtcgcgcg
     9661 gatcgccgtc gaacatcaca tcgcagatat cggttccttc ggcggccaac gctatgtcat
     9721 agctatcggg agccgaacac atagaaaaga ggtaacctcc acctgctaca aaatcacgga
     9781 ttttttttgc taccgccaac ttaagttgcg acaccttgtc gtaaccgagt ttttttgccg
     9841 tttcttccga caacctttta tcctctttat accaggcagc atctttatag tttgcccaga
     9901 atttgccgta ttgccctgta aagtcttcgt ggtgcatgtg cagccagtcg taggtgggta
     9961 aaagaccctg caacacttcc tcgtcataca ccacatcgta gggtatttcg gcataggtaa
    10021 gcaccagggt aacggcatcg tcccatggaa gcttattttt aggagagtac acggcaattt
    10081 tggggacttt aacaagcttc acatcatcca tatttatctc aggatcggca atttccgaca
    10141 gcaccagggc tgcctgagca tccgaaatca cttcgctgga aaccccgcgg atattgcatt
    10201 cgtccttaaa tacatcaaga tatttacaca taaagcttcc acctctgtaa ttaagcaacc
    10261 agctaacctc cacctcgcgc tgcaaaatcc aataagctac cccataggcc ttaaggtgat
    10321 tttgctgggc attatccatg gggatgagca aataagcgct gttgacaggc ttcacaagca
    10381 aaaaacaaca tagaatcagt aaaaattttt tcatgaatgc ttacggatag caatttatta
    10441 taacgtaaaa tactgctgct tagtataccc gacatggcaa ccagcctgtt tgcggcctat
    10501 gtcccatgga ttaaaaagga acatccacag ggggatcatc ggtctttttg gcagattttt
    10561 tattgatttt tgattgtaca attttcgttg ccggctcgta aaaggcttcg ttgggctgca
    10621 ttgccggctc ttccacgtct ttaaaatctt cataatcggt aaaccgtcca atgtttttct
    10681 ggaacctcag ctttacctcg cctacctgac cgttacggtg cttagcaaca ataatctcag
    10741 caagtccagc ggttggggtg ttatcttcaa attcttcaat tttatagtat tcaggacggt
    10801 agataaacag caccaggtcg gcatcctgct caatagcacc cgactcacgc aagtcag
//
LOCUS       DAOSFP010000022        10828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5119_length_10828_cov_4.389678, whole genome shotgun sequence.
ACCESSION   DAOSFP010000022 DAOSFP010000000
VERSION     DAOSFP010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5119_length_10828_cov_4.389678"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..2694)
                     /gene="mutS"
                     /locus_tag="PLI16_01340"
     CDS             complement(109..2694)
                     /gene="mutS"
                     /locus_tag="PLI16_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOH83233.1"
                     /translation="MKQHNAIKAKYPDALLLFRVGDFYETFGEDAIKAANILGIVLTK
                     RANGVASHIELAGFPHHALDTYLPKLVKAGLRVAICDQLEDPKLTKKIVKRGITEIVT
                     PGVAYNDKVLEHKENNFLAAIHSGPRITGVSFLDISTGEFYLSEGSKEYIDNLLQSFH
                     PNEVVLQKNKQREFSETYGDKFYTSTFEDWVFTPDFANNILQNHFQTTSLKGFGVEDL
                     EYGIIAAGAALHYLAETQHDKISHISKINRIEEEHFVWIDKFSVRNLELLHSSNERAL
                     TLVDVTDQTLTPMGSRMLKRWMVMPLKDKQLIDERLDIVGYFLKESVFTEELRKNVKN
                     IGDLERLISKVAIGKINPREIIQIKKALLSIESIKNSFEKTADTALHKIGEQLNSCKL
                     IRDRIEHDMLPDPPALANKGNVIASGVSDELDELRTLAYSGKDYLLQIQQRESEKTGI
                     PSLKIAFNNVFGYYIEVTNTHKNKVPAEWTRKQTLANCERYITEELKEYEDKILGAEE
                     KILALEARLYNDLIVALFDYIKPIQLNASLISRVDCLLSFAAVALRNNYVRPEINDSF
                     VIDIKNGRHPVIEQNLPAGEKYIANDIYLDNNHQQIIIITGPNMSGKSALLRQTALIV
                     LLAQTGCYVPADEAKIGLVDKIFTRVGASDNISSGESTFMVEMNETASILNNISSRSL
                     ILLDEIGRGTSTYDGISIAWAIAEYLHENQVFKPKVLFATHYHELNEMAKTMSGIKNF
                     HVSVKEINNKIIFLRKLAQGGTEHSFGIHVAKMAGIPQVVIDRANDLLHQLEKDHSGS
                     ELAQKGKKTQKNTEDQYQLSFIQLNDPLLEQIKDDILNTDINTLTPVEALMKLNEIKG
                     LLNRK"
     gene            complement(2970..3338)
                     /locus_tag="PLI16_01345"
     CDS             complement(2970..3338)
                     /locus_tag="PLI16_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83234.1"
                     /translation="MSTKRTCLECGEEFSGRIDKKFCSDMCRNAYNNKLNSDNTNLVR
                     NINNILRKNRRILQSLNKAEKTRVHKKKLVEKGFDFNHFTNIYRTQKGSQYYFCYEQG
                     YLPLDNDFYFLVINKRVNEE"
     gene            complement(3386..3931)
                     /locus_tag="PLI16_01350"
     CDS             complement(3386..3931)
                     /locus_tag="PLI16_01350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH83235.1"
                     /translation="MMFVPCGGVKKARQEVIGAIEMFPGANVLETGIGPGDNIRILDK
                     LFPGCSFYGIDIQKRMLKKCTRNLYKCKLDVCLYNATAEKLPFNDNSFDIVFHLGAIN
                     LFKDQKSAIQEMIRVAKPATKIVIADETQKAVNLFKIFIGDQPKVVPPVDLVPGEMQE
                     IKLSTIWKGYGYRIEFRKPLV"
     gene            4549..4911
                     /gene="ssb"
                     /locus_tag="PLI16_01355"
     CDS             4549..4911
                     /gene="ssb"
                     /locus_tag="PLI16_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH83236.1"
                     /translation="MEKSINKVELSGFAGMDPEVKTMSNGTQMLRFSLATSSSYKNSK
                     DEWVRDTTWHNIVMWGKVAELASDKIKKGCFVKLEGKIINRPYTGKNGVRQQTTEITA
                     WSFEASEHVKKSTPEKAA"
     gene            4947..5309
                     /gene="ssb"
                     /locus_tag="PLI16_01360"
     CDS             4947..5309
                     /gene="ssb"
                     /locus_tag="PLI16_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH83237.1"
                     /translation="MEKSINKVELSGFAGMDPEVKTMSNGTQMLRFSLATSSSYKNSK
                     DEWVRDTTWHNIVMWGKVAELASDKIKKGSFVKLEGKIVSRQYTDKNGAKQQTVEIMA
                     FSFDATETARKTNTEKAA"
     gene            complement(5363..6343)
                     /locus_tag="PLI16_01365"
     CDS             complement(5363..6343)
                     /locus_tag="PLI16_01365"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOH83238.1"
                     /translation="MKKNIAVLAGGYSGEYEISIKSARMLMQTLNKEKYNTYLVCITP
                     LKWVYIDENSNETPVDKNDFSIVSENNKIHFDCVFNIIHGTPGEDGKIQGYLDMLGIP
                     YTSCNQYVSALTFNKAYCNYVVESLGVSVPRSAHLIKNSTYDIGKIIDDIKLPAFVKP
                     CNGGSSFGTTKVKSAEDMEKALELAFSVDDELLIEEFINGTEITCGLFKYKNRVTVFP
                     LTEIVSNTEFFDFEAKYEGKSEEITPARISPAAATMCSATSTFLYNKLNCKGVVRIDY
                     ILTRGGKMFFLEVNTIPGMSEQSIVPQQAQVFGYSLPDFLDMLIEEALTK"
     gene            6498..7286
                     /locus_tag="PLI16_01370"
     CDS             6498..7286
                     /locus_tag="PLI16_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOH83239.1"
                     /translation="MSFFSFVKSRLFWKHTLIAFILTIVLLWVSLRLLNAYTRHGSSI
                     VVPDFKGISLASLEDFASDHDVEYIINDSLYDYSMTKGTVALQDPKPGTFVKKNRIIY
                     LTVVALKPEQVAMPNLVDLTLRQATSMLETYGLKTGSLTYVPDIAHNAVLRQKFKGVE
                     IKAGTMIEKGSRIDLVLGQDKENTSTGVPDLFGKTQQEASELLQEASLNIGNEVFLDG
                     NDEHVRVYKQQPAANASIQYGRTVDLWYRSDKKFDFNNKPEQDE"
     gene            7279..9204
                     /locus_tag="PLI16_01375"
     CDS             7279..9204
                     /locus_tag="PLI16_01375"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83240.1"
                     /translation="MNKKPLIISVLLLFCVTAFSQEVLTGLISNPALNPKHNQAFSVP
                     KQSSYVVLPLLDDFSYPNAVYPADTLWSDRKVFINSSYPVNPPTVGVATFDAINDTGA
                     IYPYGTTTPFIADTLTSLPIRLDSLLSGSSQAITAADSIYLSFYYQPQGISSDAPENA
                     DSLLLEFFSPHLNHWYRVWSAPGMKLDTFLTKYNSYFRQVLIPITDTTFIQKGFRFRF
                     KNYASLASMSLPSFQSNADQWNIDYVYLNINRTKYDTVALDVAFVDPAPTILNKYTLM
                     PARQFTNGDLKSSLQLKMSNLNNVLDNIGYQYVVKQKNGPFLHTHSGGVYDILPFITN
                     GYHTYPLHTSPAVDFTLPSMAGYDSVGFTVSHMVNSNGWIDKILTNDTITYNQDFYNC
                     FAYDDGTAENGYGLSVENAKLAYKFSLNQPDTIGGVQMYFNQTYSSPYLKYFYITIWS
                     SLDPETVIYKSKRHRPEFMDSINAYCNYLIDDTLILVSGDFYVGWQQTTDDILNIGFD
                     RNTNSKDYIFYNVDGTWANSSYNGSLMLRPLVGTKWQQTFSSIGTQDTKSGLSIYPNP
                     ATGNTVHINLPATGSISDTKEKIIIEVYNDLGQKVLHQPYSQNLDISTLNNGFYLIRL
                     SGISDTQTGFGKLIITR"
     gene            complement(9259..10428)
                     /locus_tag="PLI16_01380"
     CDS             complement(9259..10428)
                     /locus_tag="PLI16_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83241.1"
                     /translation="MNYCKVSAGALIIGLLGLTCAGQQVKTDNKTITDLSKLKLTGKV
                     KSVLESNLKAENSSGEIKTVMDRKNLSVFNASGSLLEKSEYGAEGNLNVKYTYKYDSS
                     GNRLEWTAFKADRSMRNKTTYKYDNNHHKIEENRYLSDGTLDVSHTFLYDEKGNRIMW
                     SIYKADGSLNYKETHKFNVGGLPIEICVYKPDGTLLNMDTYQYDNNGNKTEWRNYYSD
                     KSLNFTEVFMYNDKGRLIEMQHFFSGKTMAYKQTYFYDDKDNLIEEGRYKPNGTIHFK
                     NTYKYDHKGRRIENCAYKPDGTLKHKTTFRYNNQDNLIEECRYKSNGNTDYKTTWIYD
                     EYGNQTEWCKYHPDGTINFKYTYQYIYDKSGNWTRKTVLLNNVTETITERVIEYY"
BASE COUNT         3058 a         2318 c         2172 g         3280 t
ORIGIN      
        1 tccttaatca gcaagaagaa aataaattaa gttgacgcat atgcggggtg cgactattag
       61 aaagaacttg aatttagctt tggtctgcaa gtcgcgccct gattatcctc atttccggtt
      121 aagcagcccc tttatctcat tcagtttcat cagcgcctcc acgggggtga gtgtgttgat
      181 gtcggtgttc aggatgtcgt cttttatttg ttccagcaag ggatcattga gttggataaa
      241 gctgagttga tattgatctt cagtgttttt ttgtgttttt ttaccttttt gagcaagttc
      301 actgccgctg tggtcttttt cgagttgatg aagcaagtcg ttggcacggt caataacgac
      361 ctggggaatg ccggccattt ttgccacatg tatcccaaag ctgtgttctg tgcctccctg
      421 agccaactta cgaagaaaaa tgattttatt gttgatctct tttacactga catgaaagtt
      481 ctttatgccg gacatagttt tagccatttc attcagttca tggtagtgcg tggcgaacaa
      541 tactttgggt ttgaaaacct gattttcgtg gaggtattcg gcaatagccc aggcaatgga
      601 tatcccgtca taggtactgg taccgcggcc tatttcgtct aataatatga ggcttcttga
      661 agaaatgttg ttgagaatgc tggcggtttc gttcatttcg accataaacg ttgattcccc
      721 cgacgaaata ttgtcggagg cgcccacacg ggtgaagatt ttatcaacca accctatttt
      781 agcctcatcg gcggggacat aacatcctgt ttgcgccagc agcactatca atgcggtttg
      841 cctcagcaga gccgacttcc ccgacatatt gggtccggtg atgataataa tctgttgatg
      901 attattgtcc agataaatat cgttagcaat gtatttttcg cctgccggca ggttttgttc
      961 aataacaggg tgccttccgt ttttaatgtc aataacaaaa gaatcgttga tttcgggacg
     1021 cacataattg ttgcgcaatg ccaccgcggc aaaagacaac aaacaatcca cccgcgaaat
     1081 caacgaagca ttgagttgta tgggtttaat ataatcaaac aaggcaacaa tgaggtcgtt
     1141 atacagcctg gcctccaatg caaggatttt ttcttcggca cccagtattt tgtcttcata
     1201 ttctttcagt tcttccgtaa tataccgttc gcagttggca agggtttgtt tgcgggtcca
     1261 ttcggcggga actttatttt tatgggtgtt ggtcacctcg atataatagc caaacacgtt
     1321 gttgaaagct attttcagcg agggaatgcc cgttttttcg ctttcgcgct gttgtatctg
     1381 tagtagataa tctttaccgg aataggccag ggtgcgtaat tcgtcgagtt cgtcagaaac
     1441 gccgctggct atcacatttc ctttgttggc cagggcagga ggatcaggaa gcatatcgtg
     1501 ttcgatgcgg tcgcggataa gtttacagct gtttagctgt tcccctatct tatgcaaagc
     1561 ggtatcagcg gttttttcaa aactgttttt tatggattcg atggacagca aagctttttt
     1621 tatctgaatg atctcgcggg ggttgatttt cccgatagcc acctttgaga tcaggcgctc
     1681 cagatcgccg atgtttttga cattttttcg aagttcttcc gtaaagacac tttctttgag
     1741 aaagtaaccc acaatatcaa gtctttcgtc tatgagctgt ttatccttta agggcataac
     1801 catccatcgt ttcagcatgc ggctgcccat gggggtgagt gtctggtcgg ttacatcaac
     1861 caatgtcagg gctctttcgt ttgaagaatg aagcagttcc aggttgcgta ccgaaaattt
     1921 gtcaatccaa acaaaatgtt cttcttcgat acgattgatt tttgagatgt ggctgatttt
     1981 gtcgtgctgg gtttctgcca gataatgcaa tgccgcaccg gctgcaataa tgccatattc
     2041 caggtcttcc acgccaaaac ccttaagcga agtggtctga aaatgatttt ggaggatgtt
     2101 attggcaaaa tcgggagtaa aaacccagtc ttcaaaggtg gacgtataaa atttatcacc
     2161 gtaggtctcc gaaaactcac gttgtttatt tttctgcaat actacttcat tgggatgaaa
     2221 gctttgcaac aggttgtcga tgtattcttt gctgccttcg gacaaataaa actcccctgt
     2281 ggaaatatcc agaaacgaaa cccctgtgat tcgcgggccg gagtgaattg cagctaaaaa
     2341 attattttcc ttgtgttcca gcaccttgtc gttgtatgcc acgccagggg taacgatttc
     2401 ggtgatgccg cgcttgacaa ttttctttgt aagttttgga tcttcaagtt ggtcacatat
     2461 agccacgcgc agaccagctt ttaccagttt ggggaggtag gtgtcgaggg catggtgcgg
     2521 aaaaccggca agctcgatgt gagaggctac gccattagcc cttttggtga gtacaatacc
     2581 taatatgttt gccgccttga tagcatcttc accgaaagtt tcgtaaaagt cacccacacg
     2641 aaacagcaat aacgcatcgg gatatttggc tttgatggcg ttgtgttgct tcatcagcgg
     2701 ggtttcggca atatctttct tgttttcgga caaaagtgtt ttatttaaag caaaggtaat
     2761 ttatttgtaa aaaagtgcag aaaatttcta ttcttgagtc cactcaaaaa aaagtcagat
     2821 ttgataattt tcgcaacttt ttgtgtttta attcccaagg gaagttactg aaaaccagcc
     2881 acccctgcct gccccattat gtggcacgca ggctggcttt ggggcgggaa tcaggctaat
     2941 tttcacatta aataattttt ggattggact cattcttcgt ttacccgttt atttatcacc
     3001 aggaaataaa aatcattgtc gaggggcagg tagccctgct cataacagaa ataatactgg
     3061 ctgcctttct gggtgcggta aatattggta aaatgattga agtcgaaacc tttttccacc
     3121 agtttctttt tatgcaccct ggttttttcg gctttgttaa ggctttgcag tatccgcctg
     3181 ttcttgcgca ggatgttgtt gatattcctg acgaggttgg tgttgtcgct gttcagcttg
     3241 ttgttatagg cgttgcggca catgtcggaa cagaattttt tgtcgattct tccgctgaat
     3301 tcttcgccgc attcgaggca ggtgcgcttt gtgctcatct ttaaatttta aaaattactt
     3361 ttttgtgaaa tgcttatcta ttaaatcaca caaggggttt tctgaattct atccggtatc
     3421 cataaccttt ccaaatggtt gaaagtttaa tttcctgcat ctcgcctggt accaaatcaa
     3481 caggtggaac aactttgggc tgatccccga tgaagatttt aaaaaggtta acagcttttt
     3541 gtgtttcatc agcaatgaca atcttggttg caggttttgc cacccttatc atttcctgaa
     3601 ttgcactttt ttgatcttta aaaagattta tcgctcccaa atgaaatacg atgtcaaaac
     3661 tattgtcatt aaacggtagt ttttcggcag tggcattata taaacaaaca tccaacttgc
     3721 atttgtaaag atttctggta cattttttca gcattctctt ttgtatatct atcccataaa
     3781 aagagcagcc aggaaacaat ttgtccagga tgcggatatt atctcccggc ccaattcctg
     3841 tctcgagaac attggctccc gggaacattt cgatagctcc aatcacttcc tgccgtgctt
     3901 tcttaactcc tccgcagggg acaaacatca tgtttgtcag tggggtgtaa aatttattgt
     3961 aaaaaattcg aaaaatctca aatcgtttgc taaaatgaac tgcatcgtct tcgcccaaaa
     4021 aatcaataaa tctattttca tttttaaatg acttgttgca gtgcacacaa gataaagaac
     4081 attcatcaga gggttgcaaa tcatgaatct ttgccggcat taactgagca tgacatgccg
     4141 gacaacataa aatatccagg ggtaagccct tcatgaataa tacttaatta tgaattacaa
     4201 atataataaa tttcagaagg atagacaaaa ttcagactga atagttattc aatagtaaaa
     4261 tgcccgattg cacatttgta atagtctaac gaattggcct ataacaactg ttgcatcggt
     4321 attaaaacta tgaaaaattt ccttttactg tggtcaaaaa ggactaagaa aaatggttta
     4381 agaggcggct tccaaatttt caaattcgat ttatccgttt acaaacgact acaaacgact
     4441 atatccgaat acaaatattt aataaccgaa taagtttttt gggtggtggt tactttgcac
     4501 catcaatttt tcacaacgaa aatgtttaac caaaaaatac aaattatcat ggaaaaatca
     4561 atcaacaaag tagaattaag cgggtttgca ggtatggatc ccgaagtaaa aacaatgagc
     4621 aacggcacac agatgttgcg cttttcgctg gccaccagca gcagttacaa aaactccaaa
     4681 gacgaatggg tgcgcgacac tacctggcac aacatcgtaa tgtggggaaa ggtcgccgaa
     4741 ctggcttccg acaaaattaa aaaaggatgc ttcgtgaaac tcgaaggcaa aatcataaac
     4801 aggccctata ccggcaaaaa cggtgtaaga caacaaacca cagaaattac agcatggtca
     4861 tttgaagcat ctgaacatgt aaaaaaatca acacccgaaa aagcagcgta aaacattaaa
     4921 tataaaccat ttaaaaaaac ataatcatgg aaaaatcaat caacaaagta gaattaagcg
     4981 gattcgcagg tatggatcct gaagtaaaaa caatgagcaa cggcacacag atgctgcgct
     5041 tctcgctggc taccagcagc agttacaaaa actccaaaga cgaatgggtg cgcgacacta
     5101 cctggcacaa catcgtgatg tggggaaaag tcgccgaact ggcttccgat aaaattaaaa
     5161 aaggaagctt cgtaaaactc gaaggcaaaa tagtatccag gcaatacacc gataaaaacg
     5221 gcgcgaaaca acaaacagta gaaatcatgg ccttttcgtt tgacgccaca gaaacggcgc
     5281 gtaaaaccaa tacggaaaaa gctgcctgat aatttcggga aaagcaaaca aaaaggttgt
     5341 ctcggcagag gcagcctttt ttttatttag tcaaagcctc ttcaattaac atatccagaa
     5401 aatcaggcag gctgtaacca aagacctgtg cctgttgcgg tacgatactt tgttcggaca
     5461 tgcccggaat ggtgttcacc tcgaggaaaa acattttgcc acccctggta aggatataat
     5521 caatacgtac cacgccttta caatttaatt tgttataaag aaacgttgac gtggccgaac
     5581 acatggtagc cgctgctggc gaaatacgtg ccggggtaat ctcttcggat ttgccttcat
     5641 attttgcctc aaaatcaaaa aattctgtat tggacacaat ttccgtaagc gggaaaactg
     5701 tcacccggtt tttatactta aaaagaccgc atgtgatttc agttccgttt ataaactcct
     5761 ctattaacaa ctcgtcatct acactgaacg ccagttcaag ggctttttcc atatcttcgg
     5821 cagattttac cttggtggtt ccaaaactgg atccgccgtt acatggtttt acaaaagccg
     5881 gaagtttaat atcgtcaata atttttccaa tatcataagt tgaattttta ataaggtgtg
     5941 ccgacctggg cacgctaacg cccaaacttt caaccacata attgcaatag gctttattga
     6001 aagtcagggc cgagacatac tgattacaag aggtgtatgg aataccaagc atatccagat
     6061 agccctgtat cttgccatct tcgccgggtg tgccgtggat gatattaaaa acacaatcaa
     6121 aatgtatttt attgttttcc gaaacaatgg aaaaatcatt tttatcgacc ggtgtttcgt
     6181 tactattttc atcaatataa acccacttta aaggggtgat acaaacaaga taagtattat
     6241 atttttcctt gtttaaggtt tgcatcagca tccgtgcgct ctttattgat atttcgtatt
     6301 cgccggaata accgccagca agcactgcaa tgtttttctt cataaaaaaa attttgcaaa
     6361 tgtaaacaag tttcggttta tattatttac tgaccacatc tttttttgct atatttgcag
     6421 gttattaaaa taaaaacggc cgctgacagt taattaatta cggttttatt gctgtttagg
     6481 agcatttaaa taatcttatg agttttttta gttttgtaaa aagcaggttg ttttggaaac
     6541 acacgctgat agcctttata cttactattg tattgctttg ggtttcgcta cgcttattga
     6601 atgcctatac caggcacggt tcttccattg tggttcctga ttttaaggga ataagcctgg
     6661 cctctttaga ggattttgct tccgatcacg atgtggaata cattatcaat gattcgttgt
     6721 acgattattc catgacaaaa ggaaccgttg ccctgcagga tccgaagccg ggcacctttg
     6781 tgaaaaaaaa ccgcattatc taccttactg tagttgcctt aaaacccgaa caggtggcca
     6841 tgcccaacct ggtggacctc accctgagac aggctacctc gatgttggaa acttacgggc
     6901 tcaaaaccgg ctcgttgaca tatgttcccg atattgctca taacgcggtg ctccggcaaa
     6961 aattcaaagg tgtagagata aaagccggta ccatgattga aaaagggtcc cgcatcgacc
     7021 ttgtgctggg gcaggacaaa gaaaacacct ccaccggcgt gcccgacctg tttggaaaaa
     7081 cacaacaaga agcctcggaa ttgcttcagg aagcgtcgtt aaatatcgga aacgaagttt
     7141 ttcttgacgg caacgacgaa cacgtgcgtg tttacaagca acaaccggcc gccaacgcct
     7201 caatacaata tggaagaacc gttgatctgt ggtacaggtc ggataagaaa tttgatttta
     7261 ataataaacc cgaacaggat gaataaaaaa ccacttataa tcagtgtatt actgttgttt
     7321 tgtgttacgg ccttttcgca ggaggtgctt accgggctta tctcaaaccc ggcattaaac
     7381 ccaaaacaca accaggcttt ttctgttcca aaacaatctt catacgttgt acttcctttg
     7441 ctcgacgact tttcctatcc caatgccgtg tatcctgcag ataccttatg gagcgacaga
     7501 aaagttttta ttaattcttc ctatccggtt aatcccccta ctgtcggcgt ggccactttt
     7561 gatgccatca acgataccgg cgctatttat ccttatggca ccactacgcc ctttattgcc
     7621 gatacgctca cgtcgctgcc catccggctc gattctcttc tttccggctc ctcccaggcc
     7681 attactgccg ccgattccat ttatttaagt ttttattatc agcctcaggg tatcagcagc
     7741 gacgcccccg aaaacgctga ctctttgttg cttgaatttt tttccccgca cctcaatcac
     7801 tggtatcgcg tatggagcgc accgggcatg aaacttgaca cctttctgac aaaatacaac
     7861 agctatttcc ggcaagtatt gatacctatt accgatacca cttttataca gaaaggattc
     7921 aggtttcgtt ttaaaaatta tgccagcctt gccagcatga gccttccgag ttttcagagc
     7981 aatgccgatc aatggaatat tgattatgtg taccttaata tcaaccgcac aaaatatgat
     8041 actgtagccc tggacgtggc ttttgttgac cccgctccca ctatcctcaa taaatacacc
     8101 cttatgcccg ccaggcagtt taccaatggc gatctgaaaa gctcgctgca actaaaaatg
     8161 agcaacctca ataatgtatt agacaatatt ggttatcaat acgttgtgaa acaaaaaaac
     8221 ggcccgttcc tgcacaccca ttccggaggg gtttatgaca tactcccgtt tataaccaac
     8281 ggatatcaca cctatcccct gcacacgtct ccggccgtgg attttaccct tccgtcaatg
     8341 gccgggtacg acagcgtggg ctttaccgta tcacatatgg taaacagcaa cggttggata
     8401 gacaaaatcc ttaccaacga cactataact tataatcagg atttttataa ctgttttgct
     8461 tacgatgacg ggaccgctga aaacggctac ggcctttcag tggaaaacgc caaactggct
     8521 tataagtttt ctctaaatca acccgatacc attggcggag tacagatgta ttttaaccag
     8581 acctacagtt ctccttattt gaaatatttc tacatcacta tctggtcatc gctcgatccg
     8641 gaaaccgtca tttataaatc aaagcgccat cgcccggaat ttatggacag catcaatgcc
     8701 tattgtaatt accttatcga cgacaccctg atattggtaa gcggtgattt ttatgtggga
     8761 tggcagcaaa ccaccgatga cattctgaat atcggatttg acagaaacac caattctaaa
     8821 gactacattt tttataatgt tgatggcact tgggccaatt cttcctataa cgggtcgctg
     8881 atgttgcgtc cgctggtggg aacaaagtgg caacagacct tcagcagcat tggaacacaa
     8941 gacaccaaat caggcctgtc catatatccc aaccctgcca caggcaacac cgttcacatc
     9001 aatcttcccg ccaccggatc catatcagac actaaagaaa aaataattat tgaagtttat
     9061 aacgacctgg ggcaaaaagt gttgcatcaa ccctattcgc aaaatctgga tatttcgaca
     9121 ttaaacaatg gattctattt aatcaggctt tcgggtattt ctgacaccca aaccggtttc
     9181 ggcaaactca taattacccg gtaaatatta gagcaaaaag acgctgcctg tgatcaaaat
     9241 aaactaaaga aagttgtttc aataatattc tattacacgc tccgtaatag tttctgtgac
     9301 attatttaac aataccgttt tcctggtcca attgcctgac ttgtcataaa tgtattgata
     9361 ggtgtattta aaatttattg ttccgtccgg gtgatatttg caccattccg tctggttgcc
     9421 gtattcatca taaatccagg tcgttttata atccgtattc ccgtttgatt tataccggca
     9481 ttcctcaatc agattgtcct ggttgttgta gcgaaaagtg gttttgtgct tcagggtgcc
     9541 atcaggttta taggcgcaat tttcaatacg tcttcccttg tggtcgtatt tgtaagtgtt
     9601 tttgaaatgt atggtgccat tgggcttgta acggccttct tcaatcaggt tgtccttgtc
     9661 gtcatagaaa taggtttgtt tgtatgccat cgttttgccg gaaaaaaaat gctgcatttc
     9721 aatcaggcgt cctttatcgt tatacataaa aacctctgta aaattcaggc ttttgtccga
     9781 atagtaattt ctccattctg ttttattgcc attattgtca tattggtagg tatccatatt
     9841 gagcagggtg ccatcgggtt tgtaaacaca tatctctatt ggcaagcccc caacgttgaa
     9901 tttgtgagtt tctttgtaat tcagactgcc atcggcttta taaatgctcc acatgatacg
     9961 gttccctttt tcatcatata aaaaagtatg gctgacatcc agggtgccgt cggataaata
    10021 cctgttttct tctatcttat gatggttatt atcatacttg taggtagttt tgtttctcat
    10081 ggatctgtcg gccttgaaag cggtccattc gaggcggtta ccgctactgt catatttata
    10141 agtgtatttg acgttcagat ttccctcagc accatactcg cttttttcca acaaagatcc
    10201 acttgcatta aaaacagaaa gatttttacg gtccataacc gtttttattt ctccggatga
    10261 attttctgcc ttaaggtttg attccagaac agactttact tttcctgtca gttttaactt
    10321 cgacagatct gtaatggttt tattgtctgt cttaacctgt tgtcctgcac aggtaaggcc
    10381 taataaacct ataataaggg ctcctgctga tactttacaa taattcattt gttatatgat
    10441 atgccggtgc aatttatgaa aaaaaccata ataaatgccg gacccgtttc tataaattgt
    10501 tttctcaaat tgcagaatca gtttcggaat atcaaataac cggcacatgt actgccacat
    10561 acaataaaaa tctgccggtt tttattatcc caaatttcaa gactttaaaa agtagaataa
    10621 aacatctaac ttttagaatg attgtgtttt gatttgtgtg ttcatttctt ttgcttgtcc
    10681 aaaagaaatg aaccaaagaa aaagacagcc cagacaatgc ttcagcccgc tctcagaaaa
    10741 tattgaaaat ctaatcaaag taggcccgac gccgtgcgtc gggaaacccc gcaatctgcg
    10801 gggcctttga agattttcaa atattttc
//
LOCUS       DAOSFP010000023        10727 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5188_length_10727_cov_3.986694, whole genome shotgun sequence.
ACCESSION   DAOSFP010000023 DAOSFP010000000
VERSION     DAOSFP010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5188_length_10727_cov_3.986694"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLI16_01385"
     CDS             complement(<1..643)
                     /locus_tag="PLI16_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOH83242.1"
                     /translation="MDLFYRESGNGEPVIMIHGLMGMSDNWIPVARGMAGNYRMVMPD
                     LRNHGNSPHHNDFNLNVLTEDIYALCQKLKINSAVFIGHSLGGRIAMAVALQKPQMVR
                     RLIVEDIAPRRYFGNKSIARLFRIMSGLDLKGIKSFNEINEALISSLPDDKNRQLVLK
                     NIRKKGRCGYEWKLNLRVLADNLESLMAPVYGDAIYSGPTLFFKGGLSNLVTEE"
     gene            complement(647..1987)
                     /locus_tag="PLI16_01390"
     CDS             complement(647..1987)
                     /locus_tag="PLI16_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOH83243.1"
                     /translation="MPKKHTVKEKKIFVLDTSVILYNHNAIRSFEDNDIAIPITVLEE
                     LDNFKKGNEIKNFEAREFIRIIDTLSDDISLQEWIPIDGPKFGKFMVVYNGKIDLDAC
                     KVFGEDKPDHRILNAALSMMKRYPEKRVVLVTKDINLRLKAKALNIHAEDFETGKIKD
                     IDTLYTGKTLINDLPGTVIDKIYETGYCLPEDIGIKKPVPNHYFILKNTKSSVLAFFN
                     PVNGLIERVEKRQAFRITPRNAEQVFAMHAIMNPAVKLVTLQGVAGTGKTLLALAGAL
                     EQKREFKQIYLARPVVPLSNKDIGYLPGDINSKLNPYMEPLFDNLKMIKHQYDERDKE
                     FKNITEALEKDKLVITPLAYIRGRSLSNIFFIVDEAQNLTPHEVKTIITRAGENTKII
                     FTGDVFQIDTPYLDSESNGLSVLIDKIKHHPIYAHVQLEKGERSELANLAGELL"
     gene            complement(2113..2517)
                     /locus_tag="PLI16_01395"
     CDS             complement(2113..2517)
                     /locus_tag="PLI16_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83244.1"
                     /translation="MRTVLRISIFISFAIIVFACKTSQNQKLSPEKTCDLFFGYLNNY
                     EYEKAKELGTEQTVKKVSLVESLSKLGGGDKIILKDNKSQFLYCETSGKEAVCNFKTY
                     SGDIQRVYLLRQKGRWLVDLRKDAEKPANKEE"
     gene            complement(2522..2944)
                     /locus_tag="PLI16_01400"
     CDS             complement(2522..2944)
                     /locus_tag="PLI16_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HOH83245.1"
                     /translation="MRPAATCTIILFLLGVLTYSCAVFDVSYSKKPEKVAKKFLESLY
                     NGDYEKARKYGTTHTRQIIDLMDQLVTISGEKTLPVNSKVQMLDSEIRGDSAICNYFV
                     NDAKSELLLLKTDGKWLVEMKKETSGKSGGKDFLKIQK"
     gene            3254..5260
                     /gene="dnaG"
                     /locus_tag="PLI16_01405"
     CDS             3254..5260
                     /gene="dnaG"
                     /locus_tag="PLI16_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOH83246.1"
                     /translation="MIRKETIQNILDAARIDEVVGEFVSLKKRGVNLIGLCPFHNEKT
                     PSFTVSPAKGFYHCFGCGKGGGPVDFIMEHEHMSYPDALRYLAGKYNIEITDEVQTEE
                     EKAMQSELEALYNLLTFAQKHFTNNLHETEDGRAIGLTYFNERGFGPTVIKKFQLGYS
                     PAAWDYFTKTALENGYKKEYLLKTGLSIQREDGSLYDRFRDRVIFPFHNLSGRIIGFT
                     SRILTSDKNKPKYVNSPESEIYNKSKSLYGIFLAKAAISANENCYLVEGNTDVISLYQ
                     AGIENVVASSGTALTTEQIRMIRKFTNNITILYDGDAAGLKASLRGVDMILEEGLNVK
                     VVIFPENEDPDSYARKHDAIEIKRFVQDNATDFIIFKTKLLLAETKNDPIKKAELIKE
                     IVRTISLIPEPIHRNIFIKECGEVMKIQEQTLINELNKLLRRKLKKDVSQAGEKTEDD
                     NAFPEPLIYEAEKQIIEEPFDLKSFEKYLLWILLNYGTIELLIEKEIPESREHTETVP
                     VSTASYIADILSVDDFKFSTAIHQQIYDEYQEYLAKTPEDASIQFFIQHASENISKTI
                     IEILSQKYELSKYWRDKYKIYTPRHEDNDRTILINDISNTINTINLKIIGREMMEIHE
                     QIKVLQREDPNSEEIMLLLAKFSKKQHIMAKISSRLGIVINKLF"
     gene            5290..>6032
                     /locus_tag="PLI16_01410"
     CDS             5290..>6032
                     /locus_tag="PLI16_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOH83247.1"
                     /translation="MCGIAGFYSADKIFSYDHLTAMTDVLAHRGPDASGYFHEENAGL
                     GHRRLSIIDLSEAANLPMFSQDNNFVIVFNGEIYNFQEIKKELLAEKSIAFRTVSDTE
                     VVLEAFIHWGVDFVQKLNGMFAIAIYDKARKTLYLFRDRIGIKPIYYFWDGKNFAFAS
                     ELKSLTKVDVIRKNLLLNYTAVNEFLHLGYIPEPHSIYDKINKFPAGSYAVLNNTNLT
                     FTNYWDITSKIRKESNISEEEARIRLKELL"
     assembly_gap    6033..6132
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6133..6927
                     /locus_tag="PLI16_01415"
     CDS             <6133..6927
                     /locus_tag="PLI16_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HOH83248.1"
                     /translation="LARKHVTMTLSGDGGDELFLGYGAYKWANRLNNPLLKAFHKPVH
                     SCFSRLGNRFQRVGYLFDYTGFDKIKSHIFSQENLLFATREVSALLHPDLRREIILKD
                     DFSHLPLTPAEAQSLFDLHYYLKDDLLVKVDRASMNYSLETRVPILDYRIVEFAYNLP
                     EHLKLKGNIQKHLLKEVLYEYIPAHFFDRPKWGFALPLRKWLQSDLKYLIEENLSEEN
                     IRRYNLIDYKQVAQLKYCFFEKGQDYLNTRLWQLIVLQSFLDKKTF"
     gene            7306..7749
                     /locus_tag="PLI16_01420"
     CDS             7306..7749
                     /locus_tag="PLI16_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83249.1"
                     /translation="MKTKNAILTSIFAFAILMWAVTASFAQPMLSEKNPVLKPMSKNM
                     VTCPDSICCALSGPGKVVTNLDYALRTTITYPDFAEENQLWGFVVLAFRIDDCGKIDI
                     IDLNSSSVDFQIYVEEKLKGILVKDPCLYKGKTYYYRFDFMPAIG"
     gene            complement(7750..8004)
                     /locus_tag="PLI16_01425"
     CDS             complement(7750..8004)
                     /locus_tag="PLI16_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83250.1"
                     /translation="MSDNKKYRKWFYFSLGGIQVFIAIGTLPAFTIKNLTMEYRPVKK
                     KFLSIARYEIFKFTQTGHKKTRIQRVGNVTQISNFQVPVL"
     gene            complement(8047..8451)
                     /locus_tag="PLI16_01430"
     CDS             complement(8047..8451)
                     /locus_tag="PLI16_01430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HOH83251.1"
                     /translation="MISIKPSQIPGSGLGLWATKDFKKGDVIVKYDGDKISLKECERR
                     NQNQDGYGGYYLTLSKNRIIDAQYTLWAMGRYANDAAGLNRIKGLRNNAKYQLIKREA
                     FIVASRNIKAGEEILVSYGRSYWNILRKVLNK"
     gene            complement(8438..9376)
                     /gene="queG"
                     /locus_tag="PLI16_01435"
     CDS             complement(8438..9376)
                     /gene="queG"
                     /locus_tag="PLI16_01435"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOH83252.1"
                     /translation="MDTRELSFEIKKKIFDTGAAECGFARAETLQREYDFLVQWTSQG
                     LHAGKTYLERNPEVRANPALLVPGAKTVIAVLYNYYTDEKLPQHLPYRISRYAYGKDY
                     HKVINDKLAGVAAFIKQQYQSQNTRVCVDTAPIFEKAWAQRAGLGWIGKNSLLVSEKT
                     GTFHFIGIIITDVELHYDTPVEEKCGSCTLCLEACPTDALLAPHQLDVRKCISHLTME
                     QKNPLPESLRENFSGFIYGCDCCQEVCPWNHHLKPTEIREFQLCVPLKKMTAADWETL
                     TEEEFLNITEGSAIRRIGFQSLQRNIRFINEKNDQH"
     gene            complement(9383..10411)
                     /gene="ruvB"
                     /locus_tag="PLI16_01440"
     CDS             complement(9383..10411)
                     /gene="ruvB"
                     /locus_tag="PLI16_01440"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOH83253.1"
                     /translation="MAKGLLDPTKDNLTSNEIEFEKFLRPNSFSAFSGQGQVVENLKI
                     FVEAAKLRGESLDHVLLHGPPGLGKTTLSYIIANELGVGIKVTSGPVLDKPGDLAGLL
                     TNLEPNDVLFIDEIHRLSPIIEEYLYSAMEDYKIDIMIESGPNARSVQITLNPFTLIG
                     ATTRSGLLTAPLRSRFGINTRLSYYDAEILQTIIERSARILNVAIEKNAAFEIARRSR
                     GTPRIANLLLRRIRDFAQIKGSGTIDLNITNIALTALNVDKNGLDEMDIKILTTIIDK
                     FKGGPVGLTTIATAVSEDAGTIEEVYEPFLIMEGYLMRTPRGREATEKAYTHLGRINP
                     ARQNTLFE"
BASE COUNT         2864 a         2289 c         2364 g         3110 t
ORIGIN      
        1 cctcttcggt gaccaggttc gacaaaccgc ctttaaaaaa gagggtgggt cctgaatata
       61 ttgcatcacc atagacaggt gccataaggg attcgaggtt atccgcgagc actctcaggt
      121 tcagcttcca ctcatagccg cacctgcctt ttttgcggat attttttaat accaattgcc
      181 ggtttttgtc atccggcaat gaagatatca atgcttcatt gatttcatta aaggacttta
      241 tgcctttcag gtcgaggccg gacataatcc ggaacaggcg cgcgatgctt ttgttgccga
      301 aatacctgcg gggcgcaata tcttccacta tcagccgcct taccatttgc ggtttttgca
      361 aggcgacagc catggcgata cgcccaccca acgagtgtcc tataaacacg gctgagttaa
      421 ttttaagttt ttggcacaag gcatagatat cctccgtgag cacattcaaa ttaaaatcat
      481 tatggtgcgg tgagtttccg tgattgcgca gatcgggcat caccatgcgg taatttccgg
      541 ccatgcccct ggccaccggt atccagttgt cggacatccc catcagcccg tgaatcatta
      601 tcaccggttc accgttgcct gattcgcggt aaaaaagatc catacgttac aacaactctc
      661 ccgccaggtt ggctaattcg gaacgttcgc ccttttcgag ctgcacatgg gcatagatag
      721 gatggtgttt tatcttatca atcagcaccg acagtccatt actctcggag tcaaggtaag
      781 gggtatcaat ttgaaatacg tcgccggtaa agattatttt ggtgttctcc cccgccctgg
      841 taataatggt ttttacctcg tgaggcgtca ggttctgggc ttcgtccaca atgaaaaaaa
      901 tattcgacag gctccttcct cttatgtatg ccagaggagt aataacgagc ttatcttttt
      961 caagggcttc tgtgatattt ttaaattcct tatcgcgttc atcgtattga tgtttgatca
     1021 ttttgagatt atcaaacaac ggctccatat atgggttaag ttttgagttg atatcgccgg
     1081 gcaggtatcc tatatctttg ttgctcagcg gtaccacggg ccgggccagg tatatctgtt
     1141 tgaattccct tttttgttcc agggctcctg ccagcgccag taatgttttg cctgtaccgg
     1201 ccacaccctg caaggtgacc aattttacgg cgggattcat aatggcgtgc atggcgaaca
     1261 cctgttcggc gttgcgtggt gttatcctga aagcctgtct tttttccaca cgttctatca
     1321 atccgttaac gggattgaaa aaggcaagta ctgaactctt ggtattttta agaataaaat
     1381 aatgattagg aacaggtttt tttattccga tatcttcggg aaggcaatag cctgtttcat
     1441 atattttgtc gatgacggtt cccggaaggt cgtttatgag tgttttaccg gtatagagcg
     1501 tatcaatgtc tttaatcttt ccagtttcaa aatcctcggc gtgaatattc agcgcttttg
     1561 ccttcagccg caagttgata tccttggtaa ccagcaccac tcttttttcg ggatagcgct
     1621 tcatcatgga aagagccgca ttgagaatgc ggtggtcggg tttgtcctcg ccaaaaacct
     1681 tacaggcatc aaggtcgatt ttgccattat ataccaccat gaactttccg aatttggggc
     1741 cgtcgatggg tatccattcc tgcaacgaaa tatcatcgga aagtgtatcg atgatccgga
     1801 taaattcacg cgcttcaaaa ttcttaattt cgtttccttt tttgaagttg tccaattctt
     1861 cgagaacggt aatgggaatt gcgatgtcgt tgtcctcaaa acttctgatg gcattgtgat
     1921 tatagagtat cacagaggtg tcgagtacaa agattttttt ttcttttact gtgtgttttt
     1981 tgggcatagt tttttcctcg ttttttaacg ttggataaaa ataaccggat tacgataaaa
     2041 aaaaactttg ttgagccgtg agttactcac aagggaaggt taataaaagg tttgtcctga
     2101 cgaaggaatg ctttattcct ctttattagc cggcttttcg gcatccttgc gcaggtccac
     2161 cagccaacgg cctttttgcc gcaacaaata cacccgttgt atatcgcccg aatatgtctt
     2221 gaagttacat acggcctctt tgccggaggt ctcgcaataa agaaactgtg atttgttgtc
     2281 tttcaggatt attttatctc cacctcccag ttttgacaat gattccacca gactaacctt
     2341 tttcaccgtt tgctctgtac ccagctcttt ggctttttcg tactcgtagt tgttgaggta
     2401 accaaaaaaa aggtcgcagg ttttttcggg agagagtttt tgattttggc ttgttttaca
     2461 ggcaaaaaca ataattgcaa aactgatgaa aatactaatg cggagtactg ttctcattta
     2521 gttatttttg aattttcagg aaatctttgc ctcctgattt tccggaggtt tcttttttca
     2581 tttcgaccag ccattttccg tcggttttca gcagcaggag ttcgcttttc gcgtcgttga
     2641 caaaatagtt gcatattgcg ctatcgcctc ttatttcgct atcgagcatc tgcacttttg
     2701 aattaacggg caacgttttt tctccgctta ttgtaaccag ttgatccatc aggtcgataa
     2761 tttgccgggt atgggtggtt ccgtattttc ttgctttttc gtagtcgccg ttatacaaac
     2821 tctctaaaaa ctttttggca actttttccg gctttttcga ataagacaca tcaaaaacgg
     2881 cacaggagta tgtcaagact ccaagcagga acaggattat ggtacaagtg gcggctgggc
     2941 gcatggtcgg ggtgtttttt attacggttt aaaattagta cttttttgcc agtgccgggg
     3001 taaatggttt gtttttttca gaagaagatt attaatgagt ttgtcaaaat tgtttgtaaa
     3061 acaacatttt ttgagggtat ttgttttatt gcatgcgttc caaaagcgcg tgttctgaat
     3121 ggtggtcata atagccaact aaaaacaact tataggaatt tgccctaaat atgcttcggg
     3181 caagccacta agagtttttt gcccgggagc aagagatttt tttgtaactt agccgtcttg
     3241 ttttcaaccg accatgatac gcaaagaaac aatacagaat atccttgatg ctgccagaat
     3301 tgacgaggtg gtcggggaat ttgtatcgct gaaaaaacgc ggagtcaacc tgattgggtt
     3361 gtgtccgttt cataacgaaa aaacgccgtc ttttacggta tcgccggcga aagggtttta
     3421 tcattgtttt ggctgtggca aaggcggcgg gcctgtagat tttatcatgg agcacgagca
     3481 catgagttat cccgatgcct tgcggtacct ggccggcaaa tacaatatcg agattaccga
     3541 tgaagtgcaa accgaggaag aaaaggccat gcaatcagaa ctggaggcct tgtataacct
     3601 gctaacgttt gcgcaaaagc attttacaaa taatctgcac gaaaccgaag acggtcgcgc
     3661 tatcgggctg acctatttta acgaacgtgg tttcgggcct acggtaataa aaaaattcca
     3721 gcttggctat tcgccggcag cctgggatta ttttaccaaa acggcgttgg aaaacggcta
     3781 taaaaaagaa tacctgctaa aaacaggcct ttcgatacaa cgggaagacg gcagcctata
     3841 cgaccgtttc cgcgaccgcg ttatttttcc ttttcacaat ctgagcggaa gaatcatagg
     3901 gtttaccagc aggatactca cttcggacaa aaacaaaccc aagtatgtaa attcgccgga
     3961 atcggaaatt tacaacaaga gcaaaagcct ctatggtatt tttctggcca aggcggccat
     4021 tagtgccaat gagaactgct acctggtgga gggcaacacc gatgtgatat cgttatacca
     4081 ggcgggcatc gaaaatgtgg tagcttcgtc gggtactgcg ctgaccaccg agcagatcag
     4141 gatgatacgg aaattcacca acaacatcac cattttatac gatggcgatg ctgccggatt
     4201 gaaagcttcg ctgcgcggtg tagatatgat actggaagaa ggcctgaacg taaaagtggt
     4261 aatctttccc gaaaacgaag atccggattc ctatgcacgc aaacacgacg ccatcgaaat
     4321 aaaaagattt gttcaggata atgccaccga tttcataatt tttaagacca agctgttgct
     4381 tgctgaaacg aagaacgacc ctatcaaaaa agcagaactc atcaaagaaa tcgtcaggac
     4441 catctcgctg attccggagc ccatacaccg caatattttt atcaaagaat gcggcgaagt
     4501 tatgaaaatc caggagcaga cgctcatcaa cgagcttaat aaactgctcc ggcggaagct
     4561 caagaaagat gtttctcagg caggagaaaa aacagaagat gacaacgcgt ttcccgaacc
     4621 tttgatttat gaggccgaaa aacaaattat cgaagagccg tttgatctaa agtcgtttga
     4681 aaaatacctg ctgtggatat tattaaacta tggcaccata gagctgctga tagaaaaaga
     4741 aatacccgaa agccgggagc acactgaaac agtgcctgtg agcaccgctt cctacattgc
     4801 cgatatactt tcggtagatg attttaaatt cagcacggcc attcaccagc agatctatga
     4861 cgaataccag gagtaccttg ccaaaacgcc ggaagacgcc agcatacaat ttttcatcca
     4921 gcatgcctcg gagaacattt caaaaacgat tatcgagata ttaagtcaaa agtatgaact
     4981 cagcaagtac tggcgcgata aatataaaat ttacacaccc aggcatgaag acaacgaccg
     5041 gactatcctc atcaatgaca tttcgaacac catcaacact atcaatctga agattatagg
     5101 acgggagatg atggagatac acgagcaaat caaggtgctg caacgggaag accccaacag
     5161 cgaagaaatc atgctgctgc tggcaaaatt cagcaagaaa cagcacatta tggcgaaaat
     5221 aagttcgcga ttaggtatcg taatcaataa attattctaa acaacgacta tccgggggaa
     5281 aatcatacta tgtgcggcat tgcaggtttt tattcggcgg ataaaatttt ttcatacgac
     5341 catctgacgg ccatgacaga cgtcctggct caccgcgggc ccgatgcctc aggatatttt
     5401 catgaagaaa atgcaggtct tggacaccgg cggctgagca tcatcgacct gtcggaagct
     5461 gccaacctgc cgatgttctc gcaggataac aactttgtga ttgtgttcaa tggggagata
     5521 tacaacttcc aggaaataaa aaaagaactg ctggcagaaa aatcaatagc cttcagaacc
     5581 gtttcggaca ccgaggtggt gctggaagcc tttatacact ggggtgtgga ttttgtgcag
     5641 aagctcaacg gcatgtttgc cattgccatt tatgataaag ccaggaaaac cttgtacctc
     5701 ttccgcgaca gaattgggat aaaacctatc tattattttt gggacggtaa aaactttgct
     5761 tttgcctcgg aactgaaatc gttgacaaaa gtagatgtca tcagaaaaaa tctgctactg
     5821 aactacacgg ccgtaaatga atttttgcat ctgggctata ttcccgaacc gcattccatc
     5881 tatgataaaa ttaacaagtt tcctgccggc agctatgctg tgctcaacaa taccaatctg
     5941 acatttacca attactggga catcacatca aaaatcagaa aagaaagcaa cattagcgaa
     6001 gaagaagcca ggataagatt aaaagaactg ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnctggcccg caaacatgtt accatgacct tatcgggaga tggcggtgac
     6181 gaactttttt tgggatacgg cgcctacaaa tgggccaaca ggctgaacaa cccgctgctg
     6241 aaggcctttc acaagcctgt tcattcctgt ttttcgaggc tcggaaaccg ctttcagcgc
     6301 gtaggatacc tgtttgacta taccggtttc gacaagataa aaagccatat tttctcgcag
     6361 gaaaacttac tttttgccac ccgggaggta tccgcgttat tacatcctga tttacggcgg
     6421 gagataatac taaaagatga tttcagtcac cttcccctga cgccggcgga ggcgcagtcg
     6481 ctttttgacc tgcattatta cctgaaagac gacctgctgg taaaggttga ccgtgcttcg
     6541 atgaactatt cgcttgaaac ccgtgtgccc atactggatt accgtattgt tgaatttgcc
     6601 tataaccttc cggaacatct aaaattaaaa gggaacatcc aaaaacacct gctgaaagaa
     6661 gtgttgtacg aatatattcc tgcccatttc ttcgacaggc cgaaatgggg ctttgccctg
     6721 cccttaagaa aatggctgca aagcgatctc aaatacctta ttgaagagaa cctttcggaa
     6781 gaaaacatac gccggtacaa tttaatagat tacaaacagg tggcacaact taagtattgt
     6841 ttttttgaaa aaggccaaga ttatttaaac accaggctat ggcagcttat cgtgctgcag
     6901 agttttttag acaaaaagac tttttaaagg ccctaatccg gcatgcggca gctgcaaata
     6961 gaattaaaaa cgttaatctt ctgatgcaga ataaatgttt tttgtatttc ttttttattg
     7021 atatttaact cataagggag aaatgatacg ttgttcctaa tccaaacact agccaggaac
     7081 tatttgtatc atttattaat accactctgt attatttttt aatacaacat cgttgcggga
     7141 ttgatattaa tgccactcgt ggtattattg catattacag ataatctgca ttttaacttg
     7201 attttgttgc taccttttta tgcctgaatt ttttcggaac accatttgtt ttcctatttg
     7261 taaaaccaaa taaaattaaa aaagaacctt taaaaattaa ttgccatgaa aacaaaaaat
     7321 gcaattctaa catcaatttt cgcatttgcg attttgatgt gggcggttac ggcaagtttt
     7381 gctcagccca tgctctccga aaaaaacccg gtattaaaac ccatgagtaa aaacatggta
     7441 acttgtcctg actccatttg ttgtgcatta tccggtcccg gtaaggtagt gacaaatttg
     7501 gattatgcct taagaactac gattacgtat cctgactttg ccgaagaaaa ccaactttgg
     7561 ggttttgttg ttttggcctt caggatcgat gattgtggga aaattgacat cattgatctc
     7621 aactccagtt ctgttgattt tcaaatttat gttgaagaaa agctgaaagg aattctggtt
     7681 aaagacccat gcctttataa aggaaagacc tattattacc ggtttgactt tatgccggct
     7741 ataggataac tatagcacag ggacctgaaa attgcttatt tgagtcacat ttccaacccg
     7801 ctggatgcgg gttttcttat gcccggtttg tgtgaatttg aaaatttcat aacgtgcgat
     7861 tgacaaaaac ttttttttga cgggtctgta ctccatagtc aaattcttaa tagtaaaagc
     7921 aggcagggta cctatagcga tgaaaacctg tatccctccc agactaaaat aaaaccattt
     7981 cctgtatttt ttgttgtcgc tcattttttt aaattagtgt actaaaatag cctatgacgt
     8041 tggttgtcat ttattcaata cttttcttaa gatattccag taacttctgc catacgaaac
     8101 caagatttct tcaccggctt tgatattgcg tgaggcaaca ataaatgcct cccttttgat
     8161 cagctgatat ttggcgttgt tgcgcaaacc tttgatgcgg ttcaatcccg cggcatcatt
     8221 ggcatagcgt cccatggccc acagggtgta ctgggcatcg ataattctgt ttttgctaag
     8281 ggttaaatag tatccgccgt acccgtcctg gttttgattg cggcgttcgc attctttcag
     8341 ggaaatttta tcgccgtcgt atttcacgat aacatcgcct tttttaaaat ctttggttgc
     8401 ccacagccca agtcccgagc cgggtatctg tgatggttta atgctgatca tttttttcgt
     8461 ttatgaacct gatatttctt tgtagtgatt gaaagccaat tcgccgtata gccgagcctt
     8521 cggtaatgtt caaaaattct tcctctgtca gtgtttccca atctgccgca gtcatttttt
     8581 ttagcgggac gcaaagctga aattcgcgga tctcggttgg cttcaggtgg tgattccacg
     8641 gacagacttc ctggcagcaa tcgcagccat agataaaacc gctgaaattt tccctgaggc
     8701 tttccggtaa aggatttttt tgttccatcg tcaggtgtga aatgcattta cggacatcga
     8761 gttggtgcgg agccagcagg gcgtcggtgg gacaggcctc aagacacagg gtgcaggatc
     8821 cgcatttttc ttcaacgggt gtgtcgtagt gtaattcaac atcggtaatt attataccaa
     8881 taaaatgaaa agtgcctgtt ttttcgctga cgagaaggct gtttttacct atccatccca
     8941 gtcccgcccg ctgcgcccat gctttttcga atattggagc ggtatccaca caaacccggg
     9001 tattttggct ttggtattgt tgtttgatga aggctgccac accagccagt ttatcgttta
     9061 tcaccttgtg gtaatctttt ccataagcat agcgtgaaat cctgtaaggc aggtgttgcg
     9121 ggagcttttc gtccgtataa taattataaa gcacggcgat aacggttttt gcgccgggga
     9181 cgagcaacgc cggattggca cgcacttcgg gatttctttc cagataagtc tttccggcgt
     9241 gcaagccttg ggatgtccac tgtactaaaa aatcatattc ccgttgaagt gtttccgccc
     9301 tggcaaatcc acattcagct gcacctgtat cgaaaatttt tttctttatc tcaaatgaaa
     9361 gttcccgggt gtccataggg ggctattcga aaagagtgtt ttgccgtgcc gggttgatgc
     9421 gtcccaggtg ggtgtatgct ttttcggtag cttcgcgtcc ccgcggtgtc cgcatcaggt
     9481 agccctccat aatcaggaac ggctcataaa cctcttcgat ggttccggcg tcttcgctga
     9541 ccgcagtggc aatagtagta aggcctacgg gccctccttt gaatttatca atgatggttg
     9601 tcaggatttt gatatccatt tcgtcgaggc cgtttttgtc cacattcagc gctgtcagcg
     9661 caatgttggt aatattcagg tcgatggttc ccgagccttt gatctgggca aagtcgcgga
     9721 tgcggcgcaa cagcaggttg gcgatacggg gggtgccccg gctgcggcgg gcaatttcaa
     9781 aggcagcatt tttttcgata gccacgttaa gaatgcgcgc cgatcgttca ataatggttt
     9841 gcaaaatttc ggcatcataa tacgagaggc gtgtgtttat tccgaagcgt gaacgcaatg
     9901 gtgccgtgag caggcccgaa cgggtggtgg cgccgataag ggtaaaggga ttgagggtga
     9961 tttgtacgct gcgtgcattg gggccgcttt ctatcatgat gtcaatcttg taatcttcca
    10021 tggcggaata caggtattct tcaataatag gcgacaggcg gtgaatttcg tcgataaaca
    10081 gcacgtcgtt aggttcgagg ttggtcagca ggccggccag gtcgccgggt ttgtcgagca
    10141 caggccctga agtgactttg atgcctaccc cgagttcgtt agcaatgata tagctgagtg
    10201 tggttttgcc cagtcccgga gggccatgca acagcacatg atcgagcgat tcgcctcgca
    10261 gtttggctgc ttccacaaaa attttaagat tttccaccac ctgtccctga ccggaaaaag
    10321 cgctgaaaga atttggacgc aggaattttt caaattctat ttcgtttgaa gtcagattgt
    10381 ctttggtggg atccagtaag cctttagcca tcgcataacc ttttataggt gcaaagttac
    10441 caaaaatccc gcacaaaaaa cccaggcatc tgtttattgg ttaaatcttt tttaagatgc
    10501 cggagaagta gttttatttt attatttggc tgatgagtat aacttcctgc caattagatg
    10561 atgatttccc ggccgcatga ccgacgcatc gcatttgggc aggaagaggc gcgaaataac
    10621 gattcgctat tttattttac aactccccaa aaggaaataa accgtttatt ttcaaaaggc
    10681 cattttcatt gtttttatga ttcctgaaat ttatttttaa aaaagac
//
LOCUS       DAOSFP010000024        10717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5195_length_10717_cov_4.287094, whole genome shotgun sequence.
ACCESSION   DAOSFP010000024 DAOSFP010000000
VERSION     DAOSFP010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10717
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5195_length_10717_cov_4.287094"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLI16_01445"
     CDS             <1..514
                     /locus_tag="PLI16_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOH83254.1"
                     /translation="FQTNSRQALNMYNIIKEFAGDTRKKTIYDLYTGTGTIANFVALE
                     SSKVVGIEYIESAVADAKINAKLNNITNTTFIAGDIAKTLNTEFVQQHGKPDIIITDP
                     PRSGMHPAVVEQILQIKPERIVYVSCNPATQARDLALLCTQYQLKIIQPVDMFPHTHH
                     VENVVLLELK"
     gene            complement(593..2557)
                     /locus_tag="PLI16_01450"
     CDS             complement(593..2557)
                     /locus_tag="PLI16_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOH83255.1"
                     /translation="MKTKKAPKKLSAVLSVFLIIALTLFISLNTLAKGPEFKLAQSIK
                     LPFTPSLINFAHTDDFKYFLCSTKADMLMVDGTTGKILWQVNFERDFANKKFANQYWN
                     QYANVVLVFDEDTKKGVATKYFLDGKTGKLLWKSDLYVSDFGKYKLADGFRFYFDPQT
                     NGVLLPTKENVDFVDVQTGKVIWSKKFDLTGKAKEFDCAIMKYYDLVKIITGKESEIY
                     LTTIEGKEVSDIEPYFNKKKFLADRKFATVLNVPEKNMYVIMQGETSSFFAFFGGMNV
                     PSWKMNFIAYEEGSSKELWRKQYTIAYTFDWINHTPFVKMFYQDGKIFIEHDPAMKAN
                     DGLTVLDINSGQKLWECFYSTSDTKTQGLNKVLLTPFPAPDPLVIDGKAYVVDKVKNQ
                     VFCYNADNGTKIWETEKFPDAQQIPTLLKIDNILIMGYGGAAIKCAKITTTTNNVTYT
                     TYRHEYNSKDKYGIKAYDASSGNIVWSSETIAKKVKDKFSYIAGIEYVNGKLYCATDK
                     NFFILDPKTGDVSNSIPVSKEKLGASWKLYYFPKQNEIILNCQSGIVKIDPNAAIIKG
                     TVKTPNVSYYPASNDMNADDFYSDYAIFTSGDPVKGVFKTFASIDLQNMTIRGIESAS
                     LLSSSVSHFSEGGEMFYKANGSTLMIYKVK"
     gene            complement(2940..3512)
                     /locus_tag="PLI16_01455"
     CDS             complement(2940..3512)
                     /locus_tag="PLI16_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH83256.1"
                     /translation="MLLTPADFDIKPGYFIHESTVHGKLHTYRVMCHVLVLGKRMNLV
                     HQTLLAFCAALVHDMARTHDGFCIMHGPRAAKHKVPEFIPLFKKYGLTPDDINVIKTA
                     VSNHSQYFELKKDHPHYLVTSLLKDADALDRIRLGEGNLNTKYLRHEVTLELKDFSRN
                     LYIESEQITVSSFAEMLNIASKLWNTALIP"
     gene            3660..4361
                     /locus_tag="PLI16_01460"
     CDS             3660..4361
                     /locus_tag="PLI16_01460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH83257.1"
                     /translation="MKKVIVVLCIFAFYFSATAQTSKIKIKTHEDSVAYTLGCNIGAN
                     LKKNLESDSLNLSREIIAQGVRDALNGPDKMLFSEETNQKVMMQFRSEMEAKMAAKSA
                     KSASAAKAEGIAFLEENKKQAGITTTPSGLQYRVIQEGTGATPDINSTVSVHYEGKFI
                     DGRVFDSSYERNQPADFPLNGVIKGFSEALQLMKEGSIYEVFLPSDIAYGDAGNAGIP
                     GGSTLIFKIELIKVK"
     gene            4428..5672
                     /locus_tag="PLI16_01465"
     CDS             4428..5672
                     /locus_tag="PLI16_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83258.1"
                     /translation="MKKFFCCLFFFHLFFCCTGYGQTAGCTDPLATNFNAAADSNDGS
                     CVYATASVAPDTSFILSSNLSETSGLVFWNHYLWTHNDNTDLNLYSLDTASGAIVQPF
                     ALNGVANIDWEEISQDDNYIYVGDFGNNANGNRTDLKILRIEKNSLLLNAPAIDTINF
                     SYSDQTTFTPTGACNTDFDCEAFVVASDSIFLFTKQWLSKQTSLYALPKTPGTFTATL
                     KSTFNADGLITGATYLQDKKLLVLCGYSSTLAPFTWLFYDFKGYDFFGGNKRKISLAL
                     HIHQIEGIATSNGLLYYFSNEYFARYKVFTCEQKLHVVDMSLFLSNYLLGTPAGISET
                     EVITDHNNITIYPNPGKNIITVKLHRTNTAKNYFISEMTGKIVQSGNLPGDSNIIKVN
                     KLKKGTYLLKIGNDNVFKWVKE"
     gene            5705..7906
                     /locus_tag="PLI16_01470"
     CDS             5705..7906
                     /locus_tag="PLI16_01470"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOH83259.1"
                     /translation="MKTKFRAFLFIVVLLPGLVTAQVKPLYKPVTVEDLWLNYAYYPE
                     RADEIISMNDGEHYLILTNEYTIDKYAYGSPEKVSSLVTKQMLIPANDTRALKIESFE
                     LNKTETALLIKTDAEAIYRHSEISFYYVLDLTSNKLTAISDTKKQRLAEFSPDGTMVA
                     YIIDNNLYVKDLKTGNSTQITNDGLVNNIINGTTDWVYEEEFSFTKAFFWSPDSKKIA
                     FIRFDESKVKEYQMTTYGELYPEQYKYKYPVAGEDNSVVTVLVYDMSNPSAAAMRTMD
                     IGTETDQYIPRICWTEDPNTLAIFRMNRLQNKLDILLANAASGSSKVFLTQENKYYIE
                     ITDDYKFLDDGRRFIIMSELDGWSHIHMYDLNGNHVQQITRGEWDVISIKGIDEANQT
                     IYYISAESQPYNRDLYSIKFDGSGKKRLLKKDGSYEAEFSKNHQYFVGTYSDGNTPPH
                     FAIYNNKGKELQVLADNQKLKEKTEEYGFTKKEFFSFTTSGTISLYGYMIKPPDFDQN
                     KKYPVLMNVYGGPNNQYALNEWDIFDMIWYQHLAQNGYIIVCVDGRGTGSRGEQFRKC
                     TYMELGKLESEDQIETAKYLGTLPYVNSKRIGIWGWSYGGFITSLCMTKGADYFKAGI
                     AVAPVTNWRNYDNIYTERYMRKPQDNASGYDDNSPVNFVKKLKGKLLIVHGFSDDNVH
                     VQNSMELVDALVKANRQFEMQFYPNKNHGIYGGYTRYHLYKRMTDFILQNL"
     gene            complement(8068..9924)
                     /locus_tag="PLI16_01475"
     CDS             complement(8068..9924)
                     /locus_tag="PLI16_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HOH83260.1"
                     /translation="MAYKNLSDFIHTLEIAGELVKVKEYVSPVLKISEITDRVVKNGG
                     RALLFENTGTGFPVLINAFGSGKRICMALDVGELDEIARQIDELFRKVSAGKQGFIDK
                     LRMLPLLNELASWMPHTVRGRGACQEIVMQTPDMNRLPVLTCWPYDGGPFVTLPVVHT
                     IDPVSGTRNVGMYRMQVCAPDMTGMHWHLHKNSARHYHEYKKLGLRMPVSVTLGGDPV
                     YTYVATAPLPDGIDEYILAGFLRKKKVSMVKCMTNDLEVPQDVDFVIEGYVDPLEELI
                     SEGPFGDHTGFYSLPELFPRFHITCITHRQNAVYPATIVGIPPQEDAWIGKATERIFL
                     SPIRLTMHPEIIDMHMPVEGVMHNLVIIKIKKQYDGQAEKIINGLWGAGQMMFAKVII
                     VVDENTDIFNLAEVARVVSERVDVNKNIIHSKGPADILDHAAGNYAYHGKTGIDASCE
                     TVAAKGEVVIEKEKILSEFSFIKHVNDTLPGENISMVIIFIEKNKSGQIRDCADTLLA
                     GNMVQGVKSLVFMDSQAASLDLGDIAWLALNNIDPARDVFVNNAVLVVDATRKTPDFD
                     GFDRHWPNVIVSDDDTIRTVDEKWLTLGLGEFIESPSKKYKKLLWPGGAKAD"
     gene            10285..10584
                     /locus_tag="PLI16_01480"
     CDS             10285..10584
                     /locus_tag="PLI16_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83261.1"
                     /translation="MAGSSLIMPYYYISDYWYHMGEIGYGDNFASRYFIEPQSTVTYL
                     RIDIKNVDGSNYSGPGFTWDSIRVFVIPANIFRNAQIDKLDFNNHNEVTNYFKKM"
BASE COUNT         3382 a         2310 c         1990 g         3035 t
ORIGIN      
        1 tttccagacc aacagccggc aggcgctgaa catgtataat atcatcaaag aatttgccgg
       61 agataccagg aaaaaaacca tctacgacct gtacaccggc accggcacca ttgccaactt
      121 tgtggcattg gaatcctcaa aagtagttgg cattgaatat attgaatcgg ctgtggccga
      181 tgccaaaatc aacgcgaaac tcaataatat tactaacact acttttattg ccggcgatat
      241 tgcaaaaacc ctgaatacgg aatttgtcca acagcacggt aaacccgata ttatcatcac
      301 cgatccgccg cgaagcggca tgcatcctgc agtagttgag cagatactgc aaattaaacc
      361 cgagcgcatt gtttacgtaa gctgcaaccc tgcaacccag gcccgcgacc tggccttatt
      421 gtgcacacaa taccagctaa aaatcattca acccgttgac atgtttcctc acacacatca
      481 tgtggaaaat gtagttttgc tggaattgaa gtaaaaaaac cggcggagta aatgcaaaaa
      541 aaatgaggaa acagttttgt tccctcattt tcctattcct tttataatat ctctatttta
      601 ctttatatat catcaatgta ctgccattag ccttataaaa catttcacct ccttcactaa
      661 aatgcgaaac agaagacgaa agaagcgaag cactttcaat acccctgatc gtcatattct
      721 gaagatcaat ggaggcaaaa gttttaaaaa ctcctttaac agggtcaccg cttgtaaaaa
      781 tcgcataatc tgaataaaaa tcatcggcat tcatatcatt tgatgcagga tagtaagaaa
      841 cattaggcgt tttcactgtg ccctttatta tggcagcgtt aggatcaatt ttcacaattc
      901 ctgactggca attgaggatg atttcatttt gttttgggaa ataataaagc ttccacgaag
      961 cgccaagttt ttcttttgaa acagggatac tgtttgaaac atcaccggtt tttggatcga
     1021 ggatgaaaaa attcttatct gtagcacaat aaagtttacc atttacatac tcaatacctg
     1081 caatataact aaacttatcc tttaccttct tggcaatggt ttcagaactc catacaatat
     1141 taccactgga agcgtcataa gcttttatcc catatttgtc cttactgttg tattcgtggc
     1201 gataagtagt ataggtaaca ttattagtag ttgtggttat cttggcacat ttgatagcag
     1261 ctccgccata acccataata agaatattat caatcttcaa taatgtcggt atctgctgag
     1321 catcagggaa tttttcagtt tcccagattt ttgtaccgtt atctgcgtta tagcaaaata
     1381 cttggttttt cactttatcc acaacataag cttttccatc gataacaagc ggatcaggag
     1441 caggaaaggg tgtcaacagg actttattaa gcccctgcgt tttagtatct gaagtcgaat
     1501 aaaaacattc ccataatttt tgtccgctgt ttatatccaa tacagttagc ccgtcattag
     1561 ctttcatcgc ggggtcatgt tcaataaata ttttcccgtc ctgataaaac atcttgacaa
     1621 aaggggtatg attaatccag tcaaaagtat aggctatagt atattgcttc ctccataatt
     1681 ctttgctact gccttcttca taagcaatga agttcatttt ccatgatggt acgttcattc
     1741 ctccgaaaaa cgcgaaaaac gaactggttt caccctgcat aataacatac atgttttttt
     1801 cgggtacatt taaaactgta gcaaatttgc gatcggctaa aaactttttc ttgttgaaat
     1861 acggttcgat atcgcttact tcttttcctt caattgtagt gagataaatt tcactttctt
     1921 ttcccgtgat aatttttacc aggtcgtaat atttcatgat ggcacagtca aattctttgg
     1981 ctttacccgt taaatcgaat tttttactcc agataacttt tcctgtctgt acatcaacaa
     2041 aatcgacatt ttctttggtt ggcagcagga caccattggt ttgcgggtca aaataaaacc
     2101 taaaaccgtc tgccaatttg tattttccga aatccgaaac atacaagtcg ctcttccata
     2161 ataatttgcc tgttttcccg tcaagaaaat actttgtggc aacacctttc ttagtgtcct
     2221 cgtcaaaaac aagcaccaca ttggcatatt ggttccaata ctggttcgca aattttttat
     2281 tggcaaaatc cctttcaaaa ttcacctgcc agagaatttt tccggtagta ccatccacca
     2341 ttagcatatc ggctttagta gaacataaaa aatatttaaa atcatcagtg tgtgcgaaat
     2401 tgatcagaga aggtgtgaag ggaagtttaa tgctttgcgc taatttgaac tcagggcctt
     2461 tggccaaggt gttaagactt ataaacagcg tgagtgcaat aattaaaaaa actgacaata
     2521 ctgcagataa ctttttaggt gctttttttg ttttcataag gttttgttta aatttataat
     2581 taacttcata ataattatta tacgttttaa gatttatttt acttcataaa agaactttca
     2641 agcaattttc aaaaatagaa caaaaatata ttacatcaat tataaatata aatacatttt
     2701 tatcgttcaa aattactgtt taaattttaa ataagataat ttttaacaaa aaatattcat
     2761 ataattttct atattaccac ttaaacaaca aaactaataa atatttatgc ggtcatttgt
     2821 taatttagta aagtcaacta attggtagac gtatgataca atggaatgat attaaaaaaa
     2881 taaaaggaag ttcttctgca aaaaaaataa agagttattt tttattcaga ataaattttt
     2941 caagggataa gtgctgtatt ccaaagcttt gaagcaatat tcagcatttc agcaaaactt
     3001 gagacagtaa tctgctcact ctcaatataa aggtttcgtg aaaaatcttt caactccaaa
     3061 gtcacctcgt gcctcaggta tttagtattc aggtttccct cccccagcct tatccggtca
     3121 agagcatctg catctttcaa cagggatgtt accaaataat gtggatgatc tttcttaagt
     3181 tcaaaatatt gagaatgatt tgaaacagca gttttaatga cgtttatatc atcaggggtt
     3241 aaaccgtatt ttttaaataa tggtatgaat tcaggcactt tatgctttgc agccctcggg
     3301 ccgtgcatta tacagaaacc atcatgtgtt cgagccatat catgaactaa agctgcgcaa
     3361 aaagcaagca gggtctgatg aaccagattc atccttttgc ccaaaaccag cacatggcac
     3421 atgacgcggt aagtgtgcaa cttgccatga accgtgcttt catggataaa atatccgggt
     3481 ttaatgtcga agtcggcagg tgttaataac atatccaaca aaattacaac aaaacaaacc
     3541 aagcttggca aaaacgggca ttaaaagcat atcttctgca aaaaattcat taatttttat
     3601 tacttttgtt gcccttatca aaaataatta atttattcat tcaaactaat aaacacatca
     3661 tgaaaaaagt aattgtagtg ctttgtattt tcgcttttta cttctccgcc acagcgcaaa
     3721 cctccaagat taaaatcaaa acccatgagg attctgttgc ttataccctg ggttgtaata
     3781 ttggcgcaaa tctgaaaaag aacctcgaat cagattcgct gaacctcagc agggaaatta
     3841 ttgcacaggg tgtgagggac gcgctcaacg gtcctgataa aatgttattt tccgaagaaa
     3901 ccaaccaaaa agtcatgatg cagttccgtt ccgagatgga agccaaaatg gctgcaaaaa
     3961 gcgcaaaatc cgcgtcggca gccaaagcag aaggaatagc atttcttgaa gaaaacaaaa
     4021 aacaagccgg aattactacc acgccatcgg gattgcagta tcgtgtgata caggaaggca
     4081 ccggcgccac tcccgacatt aacagcaccg tttcggtaca ctacgaaggc aaatttatag
     4141 atggccgtgt ttttgattct tcatacgagc gaaaccaacc tgcagatttt ccgctaaacg
     4201 gtgttatcaa agggttttca gaagctttgc agttaatgaa agaaggcagt atctacgaag
     4261 tattcctccc ctccgacatt gcctacggcg atgcaggaaa tgcaggaata ccaggcggtt
     4321 ccacgctaat ctttaaaatc gaacttatca aagtgaaata gcggaacccg cgtcaacagg
     4381 cagttcctta tgacctgttc tgttactgtt tttgctcaaa tagcctgatg aaaaaatttt
     4441 tttgttgcct gttttttttc cacctgttct tttgttgcac gggctatgga caaaccgccg
     4501 gctgtaccga ccctttagcc acaaatttca atgcggctgc cgacagtaac gatggcagtt
     4561 gtgtttatgc cacagcatcg gtggctcccg acacctcttt tattctttca tccaatctct
     4621 ccgaaacatc agggttggta ttttggaacc attatctttg gacacacaac gacaataccg
     4681 acctgaatct atatagcctc gacaccgcca gcggcgcgat agtgcagcca tttgcgctaa
     4741 atggtgtggc aaatattgat tgggaagaaa tctcacaaga cgacaattat atttacgttg
     4801 gagatttcgg aaataacgca aacggcaacc gtacagacct gaaaattctt cgtatcgaaa
     4861 aaaactcact cctgctaaat gcgccggcta ttgacaccat caacttttcg tactcagacc
     4921 agaccacctt taccccgact ggcgcctgca atacggactt cgattgcgaa gccttcgtgg
     4981 tagcatccga cagcattttt ttatttacca aacaatggct cagcaaacaa acaagtttgt
     5041 acgcactgcc caaaaccccg ggaactttta ccgcaacttt aaaatcaaca tttaatgctg
     5101 atggattgat tacaggtgct acttatcttc aggacaaaaa actgctcgtg ttgtgcggct
     5161 acagtagcac attggcgccc ttcacctggc tgttttacga tttcaagggt tatgattttt
     5221 ttggaggtaa taaaagaaaa atatctctgg ccctgcacat acaccagata gaaggtatcg
     5281 cgaccagcaa tggcttgctt tattatttct caaatgagta ttttgcaaga tacaaggtat
     5341 ttacctgcga gcaaaaactt catgtggttg acatgagcct gtttttgagt aattatttgc
     5401 tgggaacacc ggcaggtatc agcgaaacag aagtaataac ggaccataat aacataacga
     5461 tttatccaaa ccccggtaaa aatatcatta ccgtaaaact ccaccgtacc aacactgcaa
     5521 aaaactattt catctcagaa atgaccggaa aaattgttca atcaggaaat ttgcccggtg
     5581 acagcaacat tattaaagtc aacaaactta aaaaaggcac ctacctcctg aaaatcggaa
     5641 atgataatgt gtttaagtgg gtcaaggaat aattaaaaac aacatcattc attaaaagga
     5701 ttttatgaaa acaaaatttc gtgcattttt atttattgtc gttctgctgc cagggctggt
     5761 aacagctcag gttaagccct tgtataagcc ggttacggtg gaagacctat ggcttaacta
     5821 tgcttattat cccgaaaggg ccgacgagat aatctctatg aacgacgggg aacattatct
     5881 tattttgacc aatgaatata ccattgacaa atacgcttat ggcagtcccg aaaaagttag
     5941 cagcctggta acaaaacaaa tgctgatacc ggccaacgac accagggctt tgaaaattga
     6001 atcgtttgaa ctcaataaaa ccgaaacagc cctgctgata aaaaccgacg cagaagccat
     6061 ctacaggcat tcagagattt ctttttatta tgtgctcgat ctcacctcca acaaactcac
     6121 cgccatttca gataccaaaa aacaacgact ggcagagttt tcgcccgatg gcaccatggt
     6181 cgcttatatt atcgacaaca atctgtacgt aaaagattta aaaaccggca acagcactca
     6241 aattaccaac gacgggcttg tcaataacat catcaacggg accaccgatt gggtttatga
     6301 agaagaattc agttttacca aagccttttt ctggtcaccc gacagcaaaa aaattgcgtt
     6361 tatccgtttc gacgaaagca aggtaaaaga ataccagatg actacttacg gagagctata
     6421 ccccgagcag tacaaatata aatatcctgt ggccggcgaa gacaacagtg tggtaacggt
     6481 gctggtgtac gacatgagca acccttcagc cgcagcgatg agaaccatgg atatcggcac
     6541 ggaaaccgac cagtacattc caagaatctg ctggacagaa gacccgaaca ccctcgccat
     6601 cttccgtatg aaccgcctgc aaaacaaact ggatatactg ctcgccaatg cggccagcgg
     6661 aagcagcaaa gtattcttaa cacaggaaaa caaatattat attgagatca cggatgatta
     6721 caaatttctg gatgatggca ggcgctttat cattatgagt gaactggacg gctggagcca
     6781 catccacatg tatgacctca acggaaatca cgtacaacag attacgcggg gtgagtggga
     6841 tgtgataagc ataaaaggca ttgacgaagc caatcagaca atttattaca tatcagccga
     6901 aagccagcca tataaccgcg atttgtacag tataaaattt gacggcagcg gaaaaaaaag
     6961 attgctcaaa aaagatggaa gctacgaggc agaattcagc aaaaaccacc agtactttgt
     7021 tggcacatac agcgacggca acaccccgcc gcattttgcc atttacaaca ataaaggcaa
     7081 agaattgcag gtacttgccg ataatcagaa actaaaagag aaaactgaag aatacggttt
     7141 taccaaaaaa gaatttttta gttttaccac atcgggaact atcagcctgt atgggtatat
     7201 gataaagcct cctgattttg accaaaacaa aaaatatccc gtattgatga acgtttacgg
     7261 tggccccaac aaccaatatg ccctcaacga gtgggacatt tttgatatga tctggtacca
     7321 gcacctggca caaaatggtt acatcatcgt atgtgttgac gggcgcggca ccggctcccg
     7381 gggcgagcaa ttccgtaaat gcacttatat ggaactcgga aaacttgaat ccgaagacca
     7441 gatagaaaca gccaaatatc taggcaccct gccctatgtg aattcaaaac gcattggcat
     7501 ctggggatgg agttatggcg gttttattac ttcactctgc atgacaaaag gcgccgatta
     7561 ttttaaagcc ggtattgccg tggccccggt taccaactgg cgtaactacg acaatatcta
     7621 taccgaacgc tatatgcgca aaccccagga caacgcttcg ggttatgacg ataactcgcc
     7681 ggtaaacttt gtaaaaaaat taaaaggcaa attgcttatc gttcatggtt tttccgacga
     7741 caatgttcat gtgcagaaca gtatggagct tgttgatgcg ctggtaaagg ccaacagaca
     7801 attcgagatg cagttttatc ccaataaaaa ccatggcatt tatggcggat acacccgtta
     7861 tcatctgtac aaaaggatga cggattttat tttacaaaac ctgtaataaa actgctatta
     7921 agtatctata agaaaactcg tgtttgtaca aagaaaacgt caagtttgag gcttgcgaag
     7981 cgacaaaaag cggagtttac tagtgtaaat gagcattttt attgcaagcg taacgaaaaa
     8041 attggcgttt tcttacaaac actatattta gtctgccttt gctccgcccg gccacaacaa
     8101 ttttttatat ttttttgatg gcgactcaat aaattcgccc aaacccagtg taagccattt
     8161 ttcatccaca gtccggatgg tgtcgtcatc agatacaatc acgttgggcc agtgacggtc
     8221 aaagccatca aagtcaggtg tttttcgtgt tgcatcaaca accaaaactg cattattcac
     8281 aaaaacatct cttgcaggat caatgttgtt aagcgccagc catgcaatat cccccaggtc
     8341 aagggatgct gcctggctgt ccataaacac aaggcttttg acgccctgta ccatgttgcc
     8401 ggccaacagc gtatctgcgc aatccctgat ctggccactt ttattctttt ctatgaaaat
     8461 aattaccatc gagatatttt cgccgggcaa ggtgtcattg acatgtttta tgaaagaaaa
     8521 ttcgctaagg attttttctt tttctatcac tacttcgccc ttggcagcca cggtttcaca
     8581 gcttgcatca atgcctgtct ttccatgata cgcataattc cctgctgcat gatcgagaat
     8641 atctgccggc ccctttgagt gaataatatt cttgttgaca tcaacccgtt cggaaacaac
     8701 gcgggccact tctgcaagat taaaaatatc agtattttca tccaccacga tgattacttt
     8761 cgcaaacatc atctgtcctg ctccccacaa gccatttatg attttctccg cttgtccgtc
     8821 gtactgtttt ttaatcttaa tgatcaccag attgtgcatc actccttcaa caggcatgtg
     8881 catatctata atttcggggt gcatcgtcaa ccggatcgga cttaaaaaaa tacgttcagt
     8941 agccttgcct atccaggcgt cttcctgtgg cgggataccg acgatggtag caggatatac
     9001 cgcattttgg cgatgtgtga tgcaggtaat atgaaaacgt ggaaacaact ccggcaagga
     9061 ataaaaaccg gtatgatcac cgaaaggacc ttcagaaata agttcttcca aaggatcgac
     9121 atagccttca attacaaaat caacatcttg tggcacctca agatcattgg tcatgcattt
     9181 gaccatactt acttttttct tacgcaaaaa tccggctaaa atatattcat caataccatc
     9241 gggcagcggt gctgtagcaa cataagtata cacggggtct ccgccaagag tcacgctcac
     9301 gggcatacgt aatccaagct ttttatattc atgataatgc ctggccgaat ttttatgcaa
     9361 atgccagtgc atccctgtca tatcgggtgc gcacacctgc atgcggtaca ttcccacatt
     9421 gcgagtcccg gaaacaggat cgatggtatg aaccacggga agtgtaacaa aaggaccacc
     9481 atcgtaaggc cagcaggtga gcacaggcag acggttcata tcgggggttt gcattacaat
     9541 ttcctgacaa gctcctctac ccctcaccgt atgcggcatc caggatgcca actcatttaa
     9601 taaaggcagc attcttaatt tatcaataaa accctgcttt cctgcagata cttttctgaa
     9661 taactcatca atctgacggg ctatttcatc cagttcgccg acatccagtg ccatacatat
     9721 ccttttgccg gacccaaatg cattgataag tacaggaaag ccagtgcccg tattctcaaa
     9781 cagcaaggcc ctgcccccat ttttaaccac cctgtcggtt atttctgaaa ttttcagtac
     9841 gggagaaaca tattccttca cctttaccag ttcaccggca atttcaagtg tatgaataaa
     9901 atctgaaagg tttttataag ccatagtagt gattctggaa cacaaatata atcaattaag
     9961 ggttcagccg cttcatcaaa aaagcatttg ctatgaataa ccagcacttt tatagaaaat
    10021 atgtaaataa cattgcactc aggatttcat cataatacct ttgtaaaaac ctaaaacaaa
    10081 aaaatcatga aaacattaaa actattgtcc ttgttgttta tcattgccgc aatagcatat
    10141 tcatgtgaaa aataaggccc tgccgatcct gccggtagcg atgaaacaga tggcaacgct
    10201 aatgtaacag tatatggttt tagcgggcac accttttctt tcgctaaccc gggaacaaac
    10261 tatacgctcc ctatatccgg cggaatggca ggttcatcgc tgataatgcc ctattattac
    10321 atatcggatt attggtacca tatgggcgag attggttatg gggacaattt tgcatccagg
    10381 tattttattg aaccgcaatc cactgtcaca tacctgagga tagatattaa aaatgttgat
    10441 ggatcaaatt attccggtcc tggttttaca tgggactcca taagggtatt tgtaattccg
    10501 gcaaatattt tcaggaacgc acaaatagac aagcttgatt ttaataacca caatgaagta
    10561 acaaattatt ttaaaaaaat gtagttaatt ttcagtcgaa aattttattt tagaattttc
    10621 aatattattt tagtattttt gtacataatt aataaataaa aaactatgga aaaacatcag
    10681 ggaatcatta cttttggagg gaaccctctg actcttg
//
LOCUS       DAOSFP010000025        10424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5410_length_10424_cov_4.288167, whole genome shotgun sequence.
ACCESSION   DAOSFP010000025 DAOSFP010000000
VERSION     DAOSFP010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_10424_cov_4.288167"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLI16_01485"
     CDS             complement(<1..286)
                     /locus_tag="PLI16_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83262.1"
                     /translation="MFQKGLAGAEIWNNRTSISTTDDENNPWNVEYSSSVTPFVPMDS
                     MDTRHHYYRFIQGLDVTDFLQQRSLKIKNSFPTVKNWKHFIGAGSDAHGSF"
     gene            complement(232..1485)
                     /locus_tag="PLI16_01490"
     CDS             complement(232..1485)
                     /locus_tag="PLI16_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83263.1"
                     /translation="MKHLVLSILLLIEMHSFVVFCQTRQTNTTGSDLAADVPFRMKKL
                     DANNQISSVPLHFYVHSSGCFGCNNELTDIKVWLKNADETSFNNLLTFDTYSVQAFDS
                     LLICRSTEDIAWGMQAFDSSGYEKNSQSTIRFTSDQNIFGTHYVDITKDYWYFTLTIP
                     AEKLNGLDDIFDIKVNFSLNMESDQQQYFRVFRQTEDIPRIQGWYRGDMHNHTMFSQN
                     DVEYGLPLGATKVASQKIGLDWITVTDHSSDYDNYGLTMQNNWDRLGAAVQSLNTADS
                     SFIFIRAMEASINNSNDKVVHCLLYPSPADPFCYHYFLDGGGDISSTSFSVVQALANL
                     SVYDGFAYEAHPFAENDELSFAIGGGLWNVGDTLFPANDSAFPTFGTVSCNDLSLASD
                     VYSEDTCSCSRKALQAQRYGITEHP"
     gene            complement(1569..2309)
                     /locus_tag="PLI16_01495"
     CDS             complement(1569..2309)
                     /locus_tag="PLI16_01495"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH83264.1"
                     /translation="MALIHSFERSGNALFRHRGQIPLFIFITAIPVIFLTDTTILKDT
                     VYVIITVISIVIALLGFVIRAYTIGTTPKGTSGHNTREQVAESLNTKGIYSTLRHPLY
                     LGNYLMWLGLLLFTFNVYYIIIISLFFWLYYERIMFAEERFLERKYGDEYLSWSAGVP
                     AFIPAIKKFQKSGIPFSVKSVLRREYSGVLAMAVGYFYIDHLRNFVDNRSFEPLRIST
                     YVLIASILLVLILRTLKHSGKLDESDRS"
     gene            complement(2345..4102)
                     /locus_tag="PLI16_01500"
     CDS             complement(2345..4102)
                     /locus_tag="PLI16_01500"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOH83265.1"
                     /translation="MEKTLINLTINNIPVSVQKGSTILDAAHKINMHIPTLCYHPDLK
                     VAGNCRVCVVEVAGTKELITACSTAVTEGMIVTTNSYKVRTARKTIVELLLSEHNADC
                     TKCYRNGNCELQWLASEYRIGDHVYHDLVKEEEKQPDSTSLCIIKDDSKCVRCQRCVR
                     TCSDLQDVGTFSVINKGKKQKVSTFYGKPLKDVFCIDCGQCTSRCPTAALTEKPLYEE
                     VWDALADTGKHVVIATTPAVRVAIGEELGFEYNKHSDSKIIAALRLLGFDSVVDSGYF
                     HDIYIVELCTEFMLKMKQDNENAAKTILPYITSCSPGWVEFVSTKCPEIKPLLSQCKS
                     PRLIFGSLIKTYYADKKALNPKDIITVSVTPCTAAKNESTQSMTGIADVDFSITTREL
                     VRMIRQSGIDIQRLDAEDYEPLMCEHSGASVLTEVSGGTTEAVIRTLSELVTGNELDP
                     KMLKIMPLRSEHGIKETVLTFSEVRTGWEFLKDQEIRFAAVNGSRHAMDFFKQLKENQ
                     KHYHFAEVMICQGGCIGGGGQPIPVSAELIKARASILYSLDEICGQQKAHENARLNAI
                     YREFLTKTELKENQKILYY"
     gene            complement(4114..5163)
                     /locus_tag="PLI16_01505"
     CDS             complement(4114..5163)
                     /locus_tag="PLI16_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOH83266.1"
                     /translation="MDAGLKGRGNVGFPTGMKWKFAKREESAEKYVICNANESETGTF
                     KDREILERVPRKVLSGMAICGYCTGAKKGIIYLRYEYAYLKEQLQNELNIFHEHIKKF
                     NLDFAIEIFLGGGSYVSGEESALIESMEGKRAEPRNKPPYPVISGLYGLPTVVNNVET
                     FATAQVICAIGAEEFLKLGTVYSPGSKLFSISGDTIKPGIHELELGTTIEEVVNEFGD
                     KNTKSVQVGGASGFCIPHRLFAETVIGYEGLTTGGSMIFFNNTRSMYKVLHEYLRFFA
                     EESCGLCTPCRIGTQQLLLGIEAIKRGDRPAGYMDVLKKLALNMKTSAKCGLGQSVAN
                     PFISILENFKEEILY"
     gene            complement(5286..5750)
                     /locus_tag="PLI16_01510"
     CDS             complement(5286..5750)
                     /locus_tag="PLI16_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOH83267.1"
                     /translation="MSENKSLIQEFVRVHGCGRNNLLPLLQEIVHQENFLSEEAVQAV
                     ADTLQIPASEVYGTATFYNYLDTEARGKYVIRLCRNISCMMQSQAEIVSAISLFLKIK
                     VGETTSDKKFSLLQANCLGWCHKAPAMMINNEVYTELTPQKAVQIIKEYKEK"
     gene            5941..8496
                     /locus_tag="PLI16_01515"
     CDS             5941..8496
                     /locus_tag="PLI16_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83268.1"
                     /translation="MKKTLLLILLCCAGILSANAQDSKGGTPLSFNFAGNLAEPDLVS
                     VQPPDLTMLVSEDNERVSKSEPLRIGVTLPVDFNLDNSGTWTDLPGLDASLWRLTVRC
                     KNAKAIGFGYSMFFLPEGATLFLYNKEKSRVLGAYSAENNTEDYFFSNEKIQGDEITL
                     ELLVPNDKKSAVILHITDLNYFYRTGEIFNDKGSSGACEVNVICSPEGDNWQDEKKGV
                     CLIDIKNGSNWFNCSGSLVNNTSQNCTPYVLLADHCHYYSSYPSTSDYNSWVFYFRNW
                     ASTCSGTTGSSAYSKTGCTLKAHDTYGSTGSGSDFCLVQINSAITYDVYYNGWDRTNT
                     ASSSGVGIHHPSADIMKISTYTTALTSYNAGGTGTHWRVIWAATTNGHGVTEQGSSGS
                     PIFNSAGNIVGTLTGGSSYCSTPTEPDWYGKVYYHWDQNGTTAATRLKDWLDPTNTGV
                     TSLAGVTTCGGCTAPTTQASSFTSSAISNNSMTVGWTRGNGTGGVLVVARAGSAVNAN
                     PVSGTTYTANAAFGSGTQIGTGNYVVYKGTGTFVNVTGLAAGTTYHYAVFEYNTTGTC
                     FKTPGLTGNATTTGTTIYACDTLTNILSTDTLTLYGFGTGQWGTWTGHNSYSMSEFAE
                     YYTGLTGSSISGLEVYVGHAFSGGTGGNHKITFNVYQGGGSTPGTVLGSVDVAVSALT
                     AWDINYITFASPVSFSGSDVYVGYQVYYNTPTDTFSVIQTTNRASAINSAFLKYNSSW
                     YSYPAISNPDISTALVVDPILCSPVTGIEQRDPEFGVKVYPNPANTTVYVDVNQADKS
                     TKVLIYNLQGQLIHTQAVSSRITPVNIADFNNGLYILKVESEKGVSTNKFIKE"
     gene            8611..9591
                     /gene="pfkA"
                     /locus_tag="PLI16_01520"
     CDS             8611..9591
                     /gene="pfkA"
                     /locus_tag="PLI16_01520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH83269.1"
                     /translation="MTTIKHIGVLTSGGDAPGMNAAIRAVVRTGVYMNASVTGVYRGY
                     EGLINNEFVPMQRHSVSNIIQRGGTILKTARSERFMTDEGQELAFQNLVKAKIDALVV
                     IGGDGTFRGAMALGKKYDFPIVGVPGTIDNDLYGTDFTIGYDTAINTVVEAVDKLRDT
                     AASHDRLFFVEVMGRDAGFIALRSGIACGAEEILVPETQTNIDDLIAKLENDYRENKT
                     SGIIIVAEGDDQGGAFDVARKVKEKFSYYDTRVSIIGHMQRGGTPTCMDRVLASTLGY
                     ESVMALMKNEKGVMVGQVNKTIVYTPFEKATKHHLAINHNMLKMARILSL"
     gene            9593..>10424
                     /locus_tag="PLI16_01525"
     CDS             9593..>10424
                     /locus_tag="PLI16_01525"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOH83270.1"
                     /translation="MDRITLFADVVLPLPLPGFYTYRIPFELNSTVKKGMRVVVQFGK
                     KKIYTALVYRIHENVPIDYQPKYILSVLDDIPVVNEKQYLFWDWIVKYYLCRPGEVMN
                     AALPSSLKLESESCVVLHPEFDRKFELLNDKEFLIAEALDIRQKLNLTEISDIVEQQK
                     IIPLLKNMIEKKIVLLEEELHDKYKPKTEICVRLTPKYQDEDMLHKAFDELTPKAFKQ
                     VEVLMAFIHLTRDAVPPMQHVSRQVLLGKAKAASAQLQSLIAKGILETYSQSDTRLKD
                     F"
BASE COUNT         2844 a         2318 c         2376 g         2886 t
ORIGIN      
        1 taaaggaacc atgggcatcg ctgccggcac cgataaaatg tttccagttt tttaccgtgg
       61 ggaaggagtt ttttattttg agggatcgct gctgtaaaaa atccgtaacg tccaatccct
      121 gaataaagcg gtagtaatgg tgccgtgtat ccatggaatc cataggaaca aaaggtgtca
      181 ccgacgaaga gtattccaca ttccagggat tattctcatc atcggtggtg cttatggatg
      241 ttctgttatt ccatatctct gcgcctgcaa ggcctttctg gaacaagaac aggtgtcttc
      301 gctgtacaca tcactggcaa gggagagatc gttacaactt accgtgccaa aagtaggaaa
      361 agcactgtcg ttagcaggaa acagggtgtc tcccacattc cacaggccac cgccgatggc
      421 aaagcttagt tcatcgtttt cggcgaaagg gtgtgcctca taagcaaatc catcatacac
      481 cgaaagattg gccaaggcct ggactaccga aaaactggtg gaagagatat cgcctccgcc
      541 gtcgagaaag taatggtaac aaaaaggatc ggcaggggaa ggatataaca ggcaatgaac
      601 cactttatca ttagaattat tgatgctggc ctccatagct ctgataaaaa taaagctgga
      661 gtcggcggtg ttgaggctct gcacggctgc gccaagccgg tcccagttgt tttgcatggt
      721 caggccgtag ttgtcgtaat cgctgctgtg gtcggtaacc gtaatccaat ccagtccgat
      781 cttttgagaa gctaccttgg tggctcccaa tggcaggccg tattcgacat cattttggga
      841 aaacatggtg tgattatgca tgtcgccccg gtaccagccc tgtatgcgag gaatatcttc
      901 agtctgcctg aaaaccctga aatactgctg ctggtcggat tccatattca gcgagaaatt
      961 tacttttatg tcgaagatgt catccagccc attcagtttc tcggccggaa tcgtaagtgt
     1021 aaaataccaa taatcttttg tgatatccac ataatgtgtt ccgaagatat tttgatcgga
     1081 ggtgaagcgt atggttgact ggctgttttt ttcgtatccg ctggagtcaa aagcctgcat
     1141 tccccatgca atatcttcag tggatctgca tatcaataag gagtcaaaag cctggacgga
     1201 ataggtgtca aaggtaagca gattgttaaa agatgtttca tcggcatttt ttagccacac
     1261 cttgatatcg gttagctcgt tgttacagcc aaaacaaccc gacgaatgaa cataaaaatg
     1321 taaagggaca ctgcttattt gattattggc gtcaagtttt ttcatgcgga aaggcacatc
     1381 agccgccagg tcagaaccag tggtgttggt ttgtcttgtc tgacaaaaaa caacaaagct
     1441 atgcatctca ataagtaaaa ggatgctgag tactaaatgt ttcataaaag gatttataca
     1501 aaaataaaaa taaatcacca gtaatacatt gatttttcaa catattatca gaaaaaagta
     1561 cgtgaatatt atgacctgtc agattcgtct aattttccgc tgtgcttaag tgtccgtaat
     1621 atcagcacca acaggatgga agctatcaat acataagtag atatcctcaa aggttcaaag
     1681 gagcggttat ctacgaagtt tctgagatga tcgatataaa aatatcctac agccatggcc
     1741 agcactcccg agtattcgcg gcgtaacacg cttttaacgg aaaaggggat accgcttttc
     1801 tgaaactttt tgatggcagg tataaaagca gggacaccgg ccgaccacga aaggtattca
     1861 tcaccgtatt ttctttccag aaaacgctct tcggcaaaca tgatgcgttc gtaatacaac
     1921 cagaaaaaca atgaaataat aatgatgtaa tatacattga aggtgaaaag cagcaggccg
     1981 agccacatca ggtagttgcc aagatacagc ggatgcctga gcgttgaata tattcctttg
     2041 gtattcagcg attctgctac ctgctccctg gtgttgtgcc ccgaggttcc tttaggtgtg
     2101 gtgcctatag tgtatgccct gatgacaaat cccaaaagcg caataacaat ggaaataaca
     2161 gtaattatca cgtagacggt gtctttcaaa atagttgtat ccgtgaggaa aatgacaggg
     2221 atggcggtaa taaaaataaa aagaggtatt tgtcccctgt gccggaataa tgcattgccg
     2281 gaccgttcaa aagagtgtat caaggccata gtaaaaagtt ttttagctta aaaaggcggt
     2341 aaaattaata ataaagaatc ttctggttct ctttgagttc ggttttagta agaaattctc
     2401 tgtaaatagc attcaggcgg gcgttttcat gggctttttg ctggccgcaa atctcgtcga
     2461 gtgagtacag gatagacgcc ctggctttaa taagttcggc cgataccgga ataggctgtc
     2521 cgccgccacc aatgcaccct ccctggcaaa tcattacttc tgcaaagtga tagtgttttt
     2581 ggttttcttt caattgtttg aagaagtcca tggcatgcct gctgccattg actgcggcga
     2641 atcgtatttc ctggtctttt aaaaattccc atccggttct gacctccgaa aatgtcagta
     2701 cggtttcttt tatgccgtgc tcgctgcgca gaggcataat cttcagcatt ttaggatcca
     2761 gctcgttgcc cgttaccagc tcgctgagtg tcctgatgac ggcttcggtg gttcctcccg
     2821 aaacttcggt aagtaccgag gccccggaat gttcacacat caggggttcg taatcctctg
     2881 cgtcgagcct ttgaatgtca atacctgact gacgtatcat gcgcaccagt tcgcgggtgg
     2941 tgattgaaaa atccacgtcg gctattccgg tcatgctttg ggtgctttca ttctttgctg
     3001 ccgtgcaggg tgttaccgaa accgtgatga tgtccttggg attcagggct tttttatcag
     3061 cgtaataggt cttgatcagc gatccgaaaa taagcctggg cgatttgcat tgcgataata
     3121 ggggttttat ttcaggacat ttggtgctta caaattcaac ccagcccggt gaacaggatg
     3181 tgatgtaagg taaaatggtt tttgcggcat tttcgttatc ctgcttcatt ttgagcatga
     3241 attcggtgca gagttccaca atataaatgt cgtggaaata gcccgagtcc accacgctgt
     3301 cgaatccaag cagcctgagt gcggcaatta ttttcgaatc ggaatgtttg ttgtattcaa
     3361 aaccgagttc ctcgcctata gccacacgga ctgcaggagt ggttgcaatc acaacgtgtt
     3421 tgcctgtatc agccagtgcg tcccatactt cttcgtaaag aggtttttcg gtaagcgctg
     3481 ccgtggggca acggctggta cattgcccgc aatcaataca aaaaacatcc ttcagtggtt
     3541 tgccgtaaaa agtggacact ttttgttttt tacccttatt gatgaccgaa aaggttccta
     3601 catcctgcag gtcgctgcag gtgcgcacac agcgctggca acgcacacat ttgctgtcat
     3661 ccttgatgat gcataacgag gtgctgtcgg gttgtttttc ttcctccttc accaggtcgt
     3721 gatacacatg gtcgcctatg cggtattcgc ttgccagcca ctgcaactca caattgccgt
     3781 tgcggtagca tttggtgcag tcggcattat gttccgataa caacaactcg acaatggttt
     3841 ttcgtgccgt acgcaccttg tagctgttgg tggttactat cattccctcg gtaaccgccg
     3901 tggaacaggc tgttattaac tcttttgttc cggcaacttc cacaacgcac acccggcaat
     3961 taccggccac tttcaggtcg ggatggtaac acaaggtcgg tatgtgcatg tttatcttat
     4021 gagcagcatc cagtatggtg cttcctttct gaacagaaac agggatgttg tttatggtaa
     4081 gattgatcag tgttttttcc atgtgaaaaa atattaataa agtatttctt ccttaaaatt
     4141 ttcaagtatt gaaataaatg ggttggccac ggattgcccg aggccgcatt tagcagaggt
     4201 tttcatgttt aaagctagtt ttttcaacac gtccatatag cctgccggtc tatcccctct
     4261 ttttatggct tcaataccca gcagcagttg ttgtgttcct atccggcagg gagtgcagag
     4321 tccgcacgat tcttcggcaa aaaaacgaag gtactcgtgc aagactttat acatcgatcg
     4381 cgtgttgttg aaaaaaatca tcgacccgcc ggtggtcagc ccctcatagc cgataacggt
     4441 ttcggcaaac agccgatggg ggatacagaa tccggaagcg ccgcccacct gtacagattt
     4501 tgtgttttta tctccgaatt cattgaccac ttcttcaatg gtggtgccca gctctaactc
     4561 gtgtatgccc ggcttgatgg tatcgcccga aattgaaaat aattttgatc ccggcgaata
     4621 gaccgtgccg agttttaaaa attcttcagc tcctatggca catattacct gcgccgtagc
     4681 gaaagtttcc acattgttta caaccgtagg aagtccatac agaccgctga tcacagggta
     4741 ggggggcttg tttcttggct cggcgcgttt accctccatg gattctatca gtgcgctttc
     4801 ttccccactg acataagagc cgccacccaa aaatatctct atggcaaaat caagattaaa
     4861 tttttttatg tgttcgtgaa aaatattcag ttcattctga agctgttctt ttaaataggc
     4921 atattcgtaa cgcagatata taatgccttt ttttgcgccg gtgcaatatc cgcagatagc
     4981 cattcccgat aatactttgc gggggacccg ttcaaggatt tcgcgatctt taaaagttcc
     5041 ggtttcgctt tcattggcat tgcagatcac atatttttcg gcggattcct cccgttttgc
     5101 aaatttccat ttcatcccgg taggaaaacc aacgttcccg cggcctttga gccccgcatc
     5161 catcagctcg ttgataatgt cttccggagg cctttgcggc tgcgttccag cacctgatac
     5221 cgtgaagtgg tgtaattgct gaaaataagg tcgatgcgtt tttggttaag tgtgtccata
     5281 actttttatt tctccttata ttctttgata atttgtacag ccttctgggg tgtaagttcg
     5341 gtataaacct cgttatttat catcatggcg ggggctttgt ggcaccaccc caggcagttt
     5401 gcctgaagca aggagaactt tttgtcggaa gtggtttccc ccactttaat cttgaggaac
     5461 aggcttatgg cgcttacaat ttctgcttgc gattgcatca tgcaggaaat gtttcggcac
     5521 agcctgatta cgtattttcc acgggcctcc gtgtcaagat agttgtaaaa agtagcggtt
     5581 ccgtaaactt ctgatgcggg tatctgcagg gtgtcagcca cggcctgcac cgcctcttcg
     5641 ctaagaaagt tttcctggtg tacaatctcc tgaagcaatg gcagcaaatt gttgcggcca
     5701 cagccatgta ccctgacaaa ttcttgtatc aatgatttat tttcagacat aaacttaatg
     5761 attgagttta ttattcctca aaattataaa tactacggaa ttaaacagta tgataaaata
     5821 ttatttttta acaaaatgta taaaaaatca tataatttgt taaaaaaata ttattctgtg
     5881 gcggaaaaaa aaacaacaaa tgttttattc tatatttgca tggtgaataa aattttaatt
     5941 atgaaaaaaa ctttactatt aattttattg tgttgtgccg gaattctttc ggctaacgca
     6001 caggacagca agggagggac tcccctgagt tttaattttg cgggaaattt agccgaaccg
     6061 gatctcgtca gtgttcagcc tccggacctc acgatgctcg tttcggaaga caacgaaagg
     6121 gtttcaaaaa gcgaacccct gcgtattggt gttaccctcc cggtggattt taatctggac
     6181 aattcaggaa cctggacaga tcttcccggc ctggatgctt cgctatggcg tctgactgtc
     6241 agatgcaaaa atgcaaaagc aataggtttt ggatattcca tgttttttct gcccgaggga
     6301 gctaccttgt ttctttacaa taaggaaaaa tccagggtgc tgggagccta ttctgccgaa
     6361 aacaacaccg aagattattt cttctcaaac gaaaagattc aaggcgatga aatcaccctg
     6421 gaacttcttg ttcccaatga taaaaaatct gccgtcatcc tgcatatcac cgacctgaat
     6481 tatttctatc gcacgggtga aattttcaac gataaaggca gtagcggtgc ctgcgaagtc
     6541 aatgtgattt gttcccccga aggcgataac tggcaggatg agaaaaaagg tgtctgcctc
     6601 attgatatca aaaacggttc aaactggttt aactgtagcg gttctcttgt aaacaacacc
     6661 agccagaatt gcacacctta tgtgttgtta gccgaccact gccattatta tagcagttat
     6721 ccttccacct cagattataa ttcctgggtg ttttatttca gaaactgggc gtccacctgt
     6781 agcggtacca caggctcctc ggcttattcc aaaacaggct gtacccttaa ggctcatgat
     6841 acttatgggt ccaccggcag cggctccgat ttctgtcttg ttcagatcaa cagcgccata
     6901 acctatgatg tgtattataa tggctgggac cgcaccaata cagccagttc cagcggagta
     6961 ggcattcatc atccctcagc cgatatcatg aaaatttcaa cctataccac cgccttaact
     7021 tcgtataatg caggtggcac aggtactcac tggcgggtaa tctgggcagc cacaaccaat
     7081 gggcatggag ttacagaaca gggttcttca ggttctccca tttttaacag tgcaggaaat
     7141 attgtcggaa cattgactgg cggctcttca tactgcagca caccaacaga acctgactgg
     7201 tatgggaaag tgtattatca ttgggaccaa aatggcacca cggctgctac ccgcctcaaa
     7261 gattggctgg atcccaccaa taccggtgtt acttcacttg ccggtgttac tacatgtggc
     7321 gggtgtacag cgcccacaac ccaggcatca tcatttacct cttctgcgat cagtaataat
     7381 agcatgacag ttggatggac aagaggtaac ggaaccggcg gcgttcttgt tgttgcaaga
     7441 gccggaagtg ctgtaaatgc taatcctgta agcggaacaa cctataccgc caacgcagct
     7501 tttggcagtg gcacacagat tggcacggga aattatgtcg tttacaaagg tacaggtacc
     7561 ttcgtaaatg ttaccgggct ggctgccggg acaacgtatc actatgccgt tttcgaatat
     7621 aataccaccg gtacttgttt taaaaccccg ggactgaccg gtaacgccac taccactggg
     7681 acgacaatct atgcctgcga caccctgaca aatattcttt ccacagatac cctcacattg
     7741 tatggttttg gtaccggaca gtggggaacc tggacagggc ataactctta ctcaatgagt
     7801 gaatttgctg agtattatac aggactgacc ggttcaagta tttcgggact tgaggtttat
     7861 gtcggtcatg cctttagcgg gggaacaggc ggaaatcata agattacctt taatgtgtat
     7921 cagggaggtg gttccacccc gggtaccgta ttaggatcag tagatgttgc cgtgagcgcc
     7981 ctgacagcct gggatattaa ttatattacc tttgcttcgc ccgtatcatt ttccggttct
     8041 gatgtttatg tgggttatca ggtatattat aacactccta cagatacctt ctctgtgata
     8101 caaaccacta acagggcaag tgccataaat tcggcttttt tgaaatataa cagctcctgg
     8161 tattcttatc ctgcaatcag caaccccgat atctccaccg cgctggttgt agacccaata
     8221 ctatgctctc ctgttaccgg tattgaacaa cgggatcccg aatttggtgt gaaagtgtat
     8281 cccaacccgg caaatactac agtatatgta gatgttaatc aggctgataa atccacaaaa
     8341 gtgctcattt ataatctgca gggacagtta attcacactc aggcagtatc ttcaagaatt
     8401 actcccgtca atattgctga ttttaacaat ggtttatata tcttaaaggt ggaatcagaa
     8461 aaaggagtct ccaccaataa gttcatcaaa gaatgagttt ttgactgcca ataataaaaa
     8521 cagcggacta caatagcccg ctgttttttt attcacataa caaaaactga ccatagaaaa
     8581 atttactttt gttaagaaat attttatatt atgactacaa taaaacatat tggggtgttg
     8641 acctccggcg gtgatgcccc cggcatgaat gcagccatac gggccgtggt gcgtaccggt
     8701 gtgtacatga atgcctcggt gacaggtgtc tacagaggtt atgaaggctt gataaacaat
     8761 gaatttgtgc ccatgcaacg gcattccgtt tcaaacatca ttcagcgggg aggcaccata
     8821 ctgaaaaccg cccgcagtga acgttttatg actgatgaag gacaggaact ggcatttcaa
     8881 aaccttgtaa aggcaaagat tgacgccctg gttgttattg gcggcgacgg cactttcagg
     8941 ggagctatgg cattgggaaa aaagtatgat tttccgattg tcggggtgcc gggaaccatc
     9001 gacaatgacc tctatggcac agattttacc atcggctatg ataccgccat aaataccgtg
     9061 gtggaagccg tggataaact gcgcgatact gccgcctcgc acgaccgctt gttttttgtc
     9121 gaagtaatgg gacgtgatgc cggttttatt gccctgcgca gcggaatagc ctgtggggca
     9181 gaagaaatcc tggtgccgga aacccaaacc aatatcgatg acctcatcgc aaaactcgaa
     9241 aacgattacc gcgaaaacaa aacctcgggt atcattattg tggccgaagg cgacgaccag
     9301 ggaggcgctt ttgatgtggc ccgtaaagtg aaagagaaat tcagttatta tgatacccgc
     9361 gtctccatta ttggacacat gcagcgtgga ggaactccaa cctgcatgga cagggtgctc
     9421 gcgagcactt tgggttatga atccgtgatg gctctcatga aaaacgaaaa aggagtgatg
     9481 gttggccagg ttaataaaac cattgtttat acgccatttg aaaaagccac caaacaccat
     9541 ctggcaatta atcataatat gctgaaaatg gccaggattt tatcgctgta gcatggatag
     9601 aatcacccta tttgccgatg ttgtcctgcc gctgccgctg ccgggttttt atacctacag
     9661 aatacccttt gaattgaata gcactgtaaa aaaagggatg agagtggtag ttcagttcgg
     9721 aaaaaaaaag atttatactg ctctggtgta ccgcattcat gaaaatgtcc ccatagatta
     9781 ccagcctaaa tatatattat ctgtcctgga cgatatcccg gtggttaacg aaaaacaata
     9841 tcttttctgg gactggatcg taaaatatta tttgtgccgg ccgggcgaag tgatgaacgc
     9901 ggcgctgccc tcatcgctca aacttgaaag cgagagttgt gtggtgctgc accccgaatt
     9961 tgacaggaaa tttgaactgc tcaacgataa ggaattcctg atcgcagaag cactcgacat
    10021 aagacaaaaa ctgaatctca ccgaaatatc agacattgtc gaacagcaga aaatcattcc
    10081 cctgctgaaa aacatgatag agaaaaagat tgtcctgctg gaagaagaac tgcacgacaa
    10141 atacaaaccc aaaacggaaa tatgtgttag acttacccca aagtaccagg atgaagacat
    10201 gctgcacaag gcttttgatg agctaacccc aaaagcattc aaacaggtgg aggtactgat
    10261 ggcttttatt catcttaccc gcgatgctgt tcctccaatg caacatgtta gcaggcaggt
    10321 attgctcggg aaagcaaagg ctgcatcggc gcagcttcag agcctcatag ccaaagggat
    10381 actcgaaact tattcacaaa gcgacacacg gctgaaagat tttg
//
LOCUS       DAOSFP010000026        10207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5589_length_10207_cov_4.135244, whole genome shotgun sequence.
ACCESSION   DAOSFP010000026 DAOSFP010000000
VERSION     DAOSFP010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_10207_cov_4.135244"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLI16_01530"
     CDS             complement(<1..695)
                     /locus_tag="PLI16_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH83271.1"
                     /translation="MKAVVLFFAFFMPALLFGQRFAIISDIHGATVATNEVSQLVKSW
                     NPEFIICCGDNNYACNMNIDNQVGQYYCEFISPYTGLFGLGDTVNRYFPAIGNHDTDC
                     TGISNFLSYFTLPGNERYYDFVKGDVHFFCLNSNYDEPDGVMETSVQALWLENGLANS
                     ASKYNLVYFHHPAFSSATMHGSTTYMQWPFKQWGATAVFSGHDHIYERLLIGDLFYVT
                     CGIGGGSLYSVNTP"
     gene            complement(762..3059)
                     /locus_tag="PLI16_01535"
     CDS             complement(762..3059)
                     /locus_tag="PLI16_01535"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH83272.1"
                     /translation="MNNIKLIVLYFFLSVVSVSTFAQSTFYDIDQVREVRIYFKEANW
                     DFILDSLHENVGEDGRLLGDVTVDGKMLHNIGIRYKGYSSFDPDAVKSPFNIDLEYNY
                     NNQNIQGYTKLKLSNVIQDPSFVREVLSYEIARKYVPASLANFANVYVNDTLLGLYTN
                     VEAVDKLFVDRRFGHKDNSFFKGAPENLIIPNGQNANLAYHGPDTTSYKPYYKLESDD
                     SETSWTDLLRLIDILNNDVSKIDTILNVDRTLWMHALNYVFVNLDSYIGYCQNYYLYM
                     DDFGRFNPIVWDFNMSFGSFKETDGISAPPGGLSILKRKTLDPLKMLTSNPMSPRPLI
                     VNLLQDTTRKKMFLAHLRTIINENFKNDLYLTRGQEIQSMIDAYVQSDTNKFYPYAQF
                     HLNLDTTVGSGNNVFPGIKDLMVARMAYLDSFPGISGAPVISGINHVPETPWQGTMVS
                     VTATVAGSNDVMLGYRYQTNGIFKKIKMFDDGNHNDGIAGDGVYGANILVSGTVVQYY
                     IYAQNDSAGVFSPERAEYEFYSIQPQLRQGDFVINELMAVNNAAAADQDNEYDGWIEL
                     FNATNEPQSLTNLFLSDDSLTLTKWGFPDTLVPGHGFVIVWADNDLAQQGLHTNLSLN
                     PAGGYLYVSGADNLLIDKVYYGQQIANKTFGRYPNGFGPFVYMPSSFAQNNYAGTSPV
                     KDFLVYPNPAAGTIYIEMRPDEEISGFKIYNANGQQVFERNISNCNTDSAILEIDVSR
                     FNIGLYIAVVETEKGIITKKFVVGK"
     gene            complement(3100..4353)
                     /locus_tag="PLI16_01540"
     CDS             complement(3100..4353)
                     /locus_tag="PLI16_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83273.1"
                     /translation="MKKLLFVALCILLYGIQNKVNAQFTNFTVSNSDLPSDYVSGGVA
                     VDTNNFVWVGTDAGVAKFDGTTWTVYTTTDGLPSNVIACVAVDVATNTVWVGTEGDGV
                     AKYNGSTWTSYNFADGLCDNGIHYIACDNSGTTWFGSWGAGISKFDGTTWTTYNAADG
                     FPDDQGAVASVYYIHVDASNNKWFGTDLGLVKYDNTAFTTLNQSSIPELLSNYVLTVT
                     TDASDNKWLGVQFGGLTKLSNTNAWEANYDTTNGLVDMGTQDIKFNAAGEMWLGSYTQ
                     YGALVKGGITKFNVANGTGVSLTEADGLVNDKVFRMAFDQDDALWIATAGGVSKYVDQ
                     SAIQEVGNTRTLDIYPNPVTERFYINGDINNGIATICDITGKVVLTQQVYSGVSINTA
                     HFDAGVYFIKIIEKESVYTGKFIIE"
     gene            complement(4526..6259)
                     /locus_tag="PLI16_01545"
     CDS             complement(4526..6259)
                     /locus_tag="PLI16_01545"
                     /inference="COORDINATES: protein motif:HMM:NF017722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004062 - aryl sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HOH83274.1"
                     /translation="MKKFFVAFVLFFLMDNLAHVCAQQQPLYTFVTGSSYAYLLDTAT
                     TSTSTYFARWNIGSTGYSAHFQKDTIYQSSSSKEDEGAKEAGSYSKVKKWACTSKTTA
                     TCVWTYTSSNMHHDLCPLPNGNVLIIVQESKTATQIQAAGGTYSSSANFECIKEIHPT
                     GATTGTVVWEWHLFDHLCQDNNNTKPNYTSSYAQKPERFSVDLVTGSDFFHMNGIDYN
                     PTLDQIVFSSHYKNEIYVIDHSTTTAQAATSSGGNAGKGGDFLYRWGRPQNYGCTSNG
                     NGITLNVIHDARWVPSTNALYPDYISVFHNGGCSSGHAIVLHQPPHNGYNYNYTVGQV
                     IGPATCVTPTTASFSVQNMGGAQALDNGNVLIQAPGSRFYECSGTGTTYQNVSVSSTQ
                     ADRLKLCEVRYPTATATVSNADICANTSINLGSTATSIMETSPTHTYSWTSNIGGFSS
                     SSQNPTATPETAGNYTYTVTVTNNVGCTATASVNVTVNACTDIEESQNIKPELNVYPN
                     PTSGAIELNEEFTSNNNFEIIVCNSFGEIIIREKNIKTIDLSHYANGIYYLTVKTGNA
                     DIITRKIVLIK"
     gene            6663..7727
                     /locus_tag="PLI16_01550"
     CDS             6663..7727
                     /locus_tag="PLI16_01550"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOH83275.1"
                     /translation="MNTGLKNNKSLLNILLHTGVWLILFSLPILFFPGELSSFRVVFH
                     MNWIFLFFAGILFYINYFWLVDFFVFEKKTAWFIIINILIIALFTWLNDLVIISWHKP
                     FMDGAPGPPPELGHKPMFKPDRNMIFIRNAATYVVPVIISLMLKVMKRIKKVEAEKKD
                     IEHERLQSELMHLKHQLNPHFFFNSLNNIYALVDISPEKSKEAIHRMAKLIRYLLYET
                     DRPLVSLSQEIEFLKQYVEIMKLRLTEKTNLSASFPNDKANPDIAPLLFLPLVENAFK
                     HGITSTHSEIAIELKLQNKTIIFTTQNAYNPTDNERNQAGIGIENLKKRLELLYSGQY
                     EYKTSIENGIFHTFLSIKLN"
     gene            7779..8495
                     /locus_tag="PLI16_01555"
     CDS             7779..8495
                     /locus_tag="PLI16_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOH83276.1"
                     /translation="MKTINCIIVDDEPLALDLLESYIKKTPFLHLVSRCTNAFEAIEQ
                     INNSPVDLIFLDIHMPELSGLEFSRTLSKEHRVIFTTAYEKYALEGFKVDALDYLLKP
                     FNYKEFLKAANKAAEWFEKTKATANTPLPYIYVKSEYKLIKINLDHILYIEGLKDYVK
                     IFIVDKNKPVLTLMSLKLLEQQLPHEIFMRIHRSYIINLNYIEQVERNQVVIKNTRIS
                     IADIYKESFLRHIASRSVNP"
     gene            complement(8501..9460)
                     /locus_tag="PLI16_01560"
     CDS             complement(8501..9460)
                     /locus_tag="PLI16_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH83277.1"
                     /translation="MDLSIVIPLYNEEESLPELCAWIRKVVEEQNYSYEIILVDDGSN
                     DKSWEVIKKLGSENNAIKAIRFRRNYGKSAALNVGFEASSGDVVITMDADLQDSPDEI
                     PGLYRMIKEDGYDLVSGWKKVRHDPSLSKNIPSKLYNWMTSKMSGVKLHDHNCGIKSY
                     RRSVVKSVEVYGEMHRYIPVIAKWAGFKKIGEKVVEHRKRKYGTTKFGMNRFMNGPLD
                     LMSIMFVSRFGKKPMHLFGTMGTIIFVLGFIAALYIGIQKLYYLHIEVKAPLVTNSPY
                     FYLALVAMILGTQLFLTGFLAEMVARSSSERNKYHIEENFNID"
     gene            complement(9473..10057)
                     /locus_tag="PLI16_01565"
     CDS             complement(9473..10057)
                     /locus_tag="PLI16_01565"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOH83278.1"
                     /translation="MESENIIEVPNQPKSFGSPFWKNALRCGLMLAGVIILLDLLTYI
                     FVFADMGMMVGLLISLVTIGIYIGLYIYYGKNYRNKYRNGFITYGQAFLFCLVMAVVS
                     TIVISLYYYIFYAFFDPARGINEAQKAVEIISENASIPDETKEVYIQKIMDGANPVSK
                     LTGSLQNNAIFSIVMAAIAALFIRKKEKINDVVM"
BASE COUNT         3149 a         2157 c         1860 g         3041 t
ORIGIN      
        1 ggcgtattaa ctgagtaaag actacctccg ccgattccgc aggtaacata aaacaaatca
       61 ccaatcaaaa ggcgttcgta aatatggtca tgaccggaaa ataccgccgt ggctccccac
      121 tgtttaaaag gccattgcat ataggtggtg ctcccatgca tagtggccga agaaaaagcg
      181 ggatgatgaa aatacacgag gttatatttt gatgctgagt tagccagccc attttccaac
      241 caaagcgcct gcacggatgt ttccattacg ccatcaggtt catcataatt actgttgaga
      301 caaaaaaaat ggacatcacc ttttacaaaa tcataataac gttcgttgcc gggcaaagta
      361 aaatatgata aaaagtttga aataccggta caatccgtat cgtgatttcc tatggcagga
      421 aaatacctgt tcactgtatc tcccaagcca aacaaaccgg tatagggact gataaattcg
      481 cagtaatact gccctacctg gttgtcgatg ttcatattac aggcataatt gttatcaccg
      541 caacaaataa taaattcagg attccaactt tttaccagtt gcgaaacttc attagttgca
      601 acagtggccc cgtgaatatc gctgataatg gcaaaccgct gtccaaacag caaagccggc
      661 ataaaaaaag caaaaaacaa aacaactgct ttcaatctat catgcaaata ttttttggtt
      721 cttgttctca actgattttt attcaaaaat taatgataac gctactttcc aacaacaaat
      781 tttttggtga ttatcccttt ttctgtttca acaacagcta tatataagcc aatattaaat
      841 cttgaaacat ctatttccag tatcgccgag tcggtgttgc aattactaat attccgttca
      901 aaaacctgct ggccatttgc attataaatt ttaaagccgg aaatttcttc atcaggtcgc
      961 atttcgatgt aaatcgtgcc ggcggcaggg ttaggataga ccaaaaagtc tttgacaggc
     1021 gaagtgcctg cataattatt ttgcgcaaat gatgatggca tataaacaaa agggccaaac
     1081 ccgttggggt accttccaaa ggttttattg gcgatttgct gtccataata aaccttatca
     1141 atcaaaaggt tatccgcacc ggaaacataa agataacctc ccgcaggatt taaagaaaga
     1201 ttcgtgtgca gtccctgttg cgctaaatca ttatctgccc acacaatcac aaatccgtgt
     1261 ccgggaacca gggtatcagg aaacccccat ttggtaagag taagggaatc gtcagacaaa
     1321 aacaaattag taagactctg tggctcattg gtcgcgttaa acagttctat ccaaccgtcg
     1381 tattcattat cctgatcggc agccgcagca ttattaacag ccatcaattc attaatgacg
     1441 aaatctccct gccgaagttg tggttgtatg ctgtaaaatt cgtattcggc tctttccggt
     1501 gaaaacacgc ctgcactatc gttttgagca taaatataat actgaacaac agtgcctgaa
     1561 actaaaatgt ttgcgccata aacaccatca cctgctatac cgtcgttatg attgccgtca
     1621 tcaaacattt taattttttt gaaaatgcca ttggtttgat aacggtagcc cagcataacg
     1681 tcgttgctcc ctgcaaccgt agccgtaacc gaaaccattg tgccctgcca gggagtttca
     1741 ggaacgtgat taatccccga aattacaggt gctcctgaaa tccctggaaa tgagtccagg
     1801 taagccatac gggcaaccat aagatcctta atacccggaa acacgttgtt gcccgaaccc
     1861 acggttgtgt cgaggttgag atgaaactgg gcgtagggat aaaacttgtt ggtgtcgctc
     1921 tggacataag catcaatcat gctctgtatt tcctgccccc tggtaagata caggtcgttt
     1981 ttaaaatttt cgttgatgat agtcctcaga tgagctaaaa acattttttt tcgcgtggta
     2041 tcctgcaata aattcactat caagggccgt ggcgacatgg gattggatgt caacattttc
     2101 agtggatcca gcgttttacg cttcaggatg ctcaacccgc ccggaggggc gctgatacca
     2161 tcggtttctt tgaaactacc gaacgacata ttaaaatccc agacaattgg attgaaacgg
     2221 ccaaaatcat ccatgtacag ataatagttc tggcagtaac caatgtagct gtcgaggttc
     2281 acaaaaacat aattgagtgc gtgcatccac aaggtacgat ccacattcag aatagtgtca
     2341 attttagaaa cgtcattgtt taaaatatcg atgagtctca gcaggtcggt ccaggaggtt
     2401 tccgaatcat cggattccaa tttataataa ggtttgtagc ttgtggtgtc gggtccgtga
     2461 taggccaggt ttgcattttg tccgtttgga ataataagat tttcgggagc ccccttaaaa
     2521 aaactattat ccttatgccc aaaacgcctg tcaacaaaca gtttgtccac cgcctccaca
     2581 ttggtgtaca ggcccagcag ggtatcattc acatagacat tagcaaaatt ggcaagtgaa
     2641 gccggcacat attttctggc tatttcatac gacaacactt cgcgtacaaa tgagggatcc
     2701 tgaataacat tactcaactt caatttagta taaccctgaa tattctggtt gttatagttg
     2761 tattcaaggt caatattgaa aggacttttt acggcatccg gatcgaaaga actatatcct
     2821 ttataccgga taccgatgtt atggagcatt ttgccgtcaa ccgttacatc gcccaaaagg
     2881 cgaccgtctt cgccaacatt ttcatgcaaa ctatccagga taaaatccca atttgcttct
     2941 ttgaaatata tccggacttc tcttacctga tcaatatcgt aaaaagtgct ttgcgcgaaa
     3001 gttgataccg atacaacgga taggaaaaaa tataaaacta tcagtttaat gttattcaaa
     3061 atccggataa ttaacacgtt taaaaagcct caacaatttc tattcaataa taaatttacc
     3121 agtgtaaacg ctttcttttt cgatgatttt gataaaataa acacctgcat caaagtgagc
     3181 tgtatttatt gacacaccgg agtaaacctg ctgagttaag actacttttc ctgtgatatc
     3241 gcatatcgta gctataccat tattaatatc tccgttgatg taaaatcttt cagttacagg
     3301 atttggatag atatcaaggg ttctggtgtt acctacttcc tgtattgccg attgatcaac
     3361 atattttgaa acgcctcctg ctgtggcaat ccataaagca tcatcctgat caaaagccat
     3421 cctgaacacc ttatcattta ccaggccatc ggcctctgtc aatgacactc ccgtaccatt
     3481 ggcgacattg aattttgtaa taccgccttt gactaatgcg ccatactgtg tgtatgatcc
     3541 aagccacatt tcgcctgctg cattgaattt tatatcctgt gttcccatat cgaccaatcc
     3601 gtttgttgtg tcgtaattag cttcccaggc atttgtgttg cttaatttgg tcaggccgcc
     3661 aaactgaacg cccaaccact tattatcact ggcatcagta gtaacagtca gcacataatt
     3721 gctgagcaat tcgggaatag agctctgatt taaagttgta aatgctgtat tgtcgtattt
     3781 cactaacccc agatcggttc caaaccattt attgtttgaa gcatcaacgt gaatataata
     3841 caccgaagcc acggccccct gatcatcagg aaatccatcc gccgcattat aagttgtcca
     3901 tgtggttccg tcaaactttg aaataccggc accccaggag ccgaaccatg tggttcccga
     3961 gttatcgcaa gcgatgtaat gaattccatt atcacataaa ccgtctgcaa aattgtaact
     4021 tgtccatgtt gaaccattat atttggctac accatcacct tcggtgccaa cccatacggt
     4081 attagtagct acatccacgg caacacaggc aataacattg gaaggaaggc catcggtggt
     4141 tgtatataca gtccaggttg ttccgtcaaa tttagcgaca ccggcatcgg tgccaaccca
     4201 aacaaaattg ttggtgtcca ctgcaactcc gccgctaaca taatcgctgg gcagatcgct
     4261 gttgctaacc gtaaaattgg tgaactgggc attcaccttg ttttgaatcc cataaagcag
     4321 gatacataat gcaacaaata gtaatttttt cattttagta tttttaggtt taaaaaaata
     4381 tctgtctgcg ataaaaatct tagcgaaatt aagaaggatt ttatatgatc aatgataatt
     4441 ctttggacaa aaatgacagt tttttatcat aataaaaatc aagattccat tttatccaca
     4501 atttttataa atttttttcg aatatttatt ttatcaaaac aatctttcgg gtaatgatat
     4561 cagcattacc ggtttttaca gtaagataat aaataccgtt agcataatgc gaaaggtcaa
     4621 tggtttttat attcttttcc ctgatgataa tttctccaaa agaattacaa actataattt
     4681 caaagttatt attgcttgta aactcctcat tcagttcaat ggcgcctgat gttggattag
     4741 gataaacatt cagttcgggt ttaatgtttt gactttcctc aatatcggta caggcattaa
     4801 cggtaacatt gaccgatgca gttgcggtac aacccacatt attggtaacg gtaaccgtat
     4861 aggtatagtt ccctgctgtt tccggggtag ctgtggggtt ttgggatgat gaggaaaaac
     4921 cgccgatatt tgaagtccat gaataggtgt gtgtcgggct tgtttccatt atggaagtgg
     4981 ccgttgatcc taagttaatt gatgtattgg cacatatatc tgcattagag acagtggctg
     5041 tggctgtagg atatctgacc tcgcacaatt taagcctgtc agcctgggta gaggaaacgg
     5101 aaacattttg ataggtagtg ccggtaccgc tgcattcata aaacctcgag cccggagcct
     5161 gtatcagcac atttccgtta tccagtgcct gagcgccacc catattttgc acagaaaaag
     5221 aagcggtggt aggagttaca catgtggcag ggcctatcac ctgcccaacc gtgtaattat
     5281 aattataacc gttatggggt ggctggtgca atacgatggc gtgtccgcta ctacaaccac
     5341 cattatgaaa cacactgata taatcgggat aaagtgcatt ggtagaaggc acccagcggg
     5401 catcatgaat cacattaagc gtaattccat ttccgttaga agtacatccg tagttctgcg
     5461 gccttcccca acggtacaga aaatcgcctc ctttgccggc attaccaccc gagctggtag
     5521 ctgcctgtgc cgtagtcgta ctatgatcga taacatatat ttcattttta tagtgcgaac
     5581 tgaaaacaat ctgatcaagt gttggattgt aatctatacc gttcatatga aaaaagtcgg
     5641 aaccagtaac caaatctaca ctgaaacgtt cgggtttttg agcatacgat gaagtataat
     5701 tcggtttagt gttgttatta tcctggcaca agtggtcaaa caagtgccat tcccaaacaa
     5761 cagttccggt agtggcccct gtagggtgta tttccttaat acattcaaaa tttgcactac
     5821 ttgaatatgt accgccagca gcctgtatct gagtagctgt ttttgattcc tgaacaatta
     5881 tcaggacatt tccgtttggc aaagggcata ggtcatgatg catgttggaa gaggtatatg
     5941 tccagacaca agtagcagtt gtcttgctgg tacaggccca tttttttact ttggaataac
     6001 taccggcttc tttcgctcct tcatcctctt tggaggacga actctgataa attgtatcct
     6061 tttgaaaatg cgcagaatat cctgttgaac ctatattcca acgggcaaaa taagtagatg
     6121 tggaggttgt ggcagtgtct aacaaatatg cataactgct tccggttacg aatgtatata
     6181 acggttgctg ctgggcacac acatgcgcca aattatccat taaaaaaaac aatacaaaag
     6241 cgacaaaaaa cttcttcata cgtttaaatt tcattaatac taattttcgt tattttcttt
     6301 aggagttcga tagttttcct taaaaatacg tgctttttaa ttaataactt acatcaaaac
     6361 ttatcacaca aaaataactc attttcaaaa taaatttatc atatctcaat taaaatatac
     6421 gttctttaca aaataatcga tgaaatgaca ttgtttatcg acacaatgtt tatcaataaa
     6481 ttaatcagcc attttactaa tgaattaatt ttatattttc atttctaaca ttaaaacgat
     6541 ttattgatgg ctttaaaaga taaaaaaaaa ttatacctct ataaattatc cccttacatc
     6601 tattttactt taaataattg aattttattc agacttttgt attgtaaatc aataaccttt
     6661 ttttgaacac cggcctgaaa aataataaat cgctattaaa catactgctc cacaccggcg
     6721 tatggttaat acttttcagc ctgccaatat tgttttttcc cggagagtta agttctttca
     6781 gagtggtgtt ccacatgaac tggatttttt tatttttcgc tgggatcttg ttttatatca
     6841 actacttttg gctggtagat ttttttgttt tcgaaaagaa aacagcttgg ttcattatta
     6901 tcaacattct gatcatagcc ttgtttacct ggcttaacga ccttgtgata atttcctggc
     6961 ataaaccttt tatggacggt gcacccggac cgcctcccga actcggacac aagccaatgt
     7021 tcaaacccga cagaaacatg atatttatca ggaatgctgc cacttacgtc gtcccggtta
     7081 tcataagcct gatgttaaaa gtcatgaaaa gaattaaaaa agtggaagca gaaaaaaaag
     7141 atattgaaca cgaacgactg caatcagagc tgatgcacct aaaacaccaa ctgaatccac
     7201 atttcttttt caattcacta aacaatattt acgcactggt cgacatatcc cccgaaaaat
     7261 ccaaagaagc catacaccgc atggctaagc tgattcgcta tttactctat gaaactgatc
     7321 gacctctggt aagtctttca caggaaattg aatttctgaa acaatacgtt gaaatcatga
     7381 aattacgcct gaccgaaaaa accaatttat ccgcctcctt tccaaatgac aaagccaatc
     7441 cggatatagc cccgcttctg tttctgcctt tggtggaaaa tgcttttaaa catggcatca
     7501 caagtacaca ttcggaaatt gcaattgaac tgaagctgca gaacaaaacc ataattttta
     7561 cgacccaaaa tgcctacaat cctaccgata atgaaagaaa ccaagcaggc atcggtatcg
     7621 aaaacctaaa aaaaagatta gagcttttgt attcaggaca gtatgaatat aaaacctcta
     7681 tagaaaatgg aatatttcac acctttttgt caataaaact caactaatag taatctgaat
     7741 tagtaaatca gtacctttac cttttgtttt atctctaaat gaaaactatt aattgtatta
     7801 tagttgatga tgagccgctg gcgcttgatc tgcttgaaag ttatatcaaa aagacgccgt
     7861 tccttcatct cgttagccgg tgtaccaatg cttttgaagc cattgaacaa atcaataaca
     7921 gcccggtaga tttaatcttt ttggatattc acatgccgga attgagcggc cttgaatttt
     7981 cacgtacgtt atcaaaagaa cacagggtga tcttcaccac ggcctacgaa aaatacgccc
     8041 tggaagggtt taaagtcgat gcgttggatt atttgctcaa accgtttaat tataaagagt
     8101 ttttgaaagc cgcaaacaaa gccgcggaat ggtttgagaa aaccaaagca acagcaaata
     8161 ctcccctgcc ctacatttat gtaaaatcag aatacaaact gataaaaatc aatctggatc
     8221 atattttata tatagaaggg ttgaaagact atgtaaagat ttttatagta gataaaaata
     8281 aaccggtatt aacattgatg agtttgaagt tgctcgagca acaattgccc catgaaattt
     8341 tcatgcggat acatcggtct tatattatca atctaaacta tattgaacag gttgagcgca
     8401 atcaggtagt tataaaaaac accagaatca gtattgcgga tatttataaa gaaagttttt
     8461 taaggcacat agcctctcgg tcggtaaacc cctaatatca tcaatcaata ttgaaattct
     8521 cttcgatgtg atatttgttt cgctcactgg aactgcgggc aaccatttcg gccagaaatc
     8581 ctgttaaaaa aagttgtgtt cccaaaatca tcgctaccag tgccagataa aaatacgggc
     8641 tgttggtaac caaaggggct tttacctcga tatgcaaata atacaacttt tgaataccaa
     8701 tatataaggc agcaataaaa ccaagcacaa aaataatcgt tcccatagtg ccgaacaaat
     8761 gcataggttt tttgccaaat ctcgacacaa acattatgga catcaggtcg agcgggccat
     8821 tcataaagcg gttcatgcca aattttgtgg tgccatattt tcgtttgcgg tgttccacca
     8881 ccttctctcc tatcttttta aaacccgccc atttagcaat tacaggaata tagcggtgca
     8941 tttcaccata cacttccaca ctctttacaa cactacgacg gtatgacttt atgccgcaat
     9001 tatggtcgtg aagcttcacc cccgacattt tactggtcat ccagttgtat aattttgatg
     9061 gaatattttt gctgagtgaa gggtcatgtc tgactttttt ccagcccgaa acaaggtcgt
     9121 aaccatcttc ttttatcatc cggtagaggc cgggaatctc atcaggactg tcttgcaaat
     9181 cagcatccat agtaatgaca acatccccgc ttgatgcctc aaaacccacg tttaatgcag
     9241 ccgacttgcc ataattcctt cgaaacctaa tggctttgat cgcattgttt tcgctgccaa
     9301 gtttttttat cacttcccag gatttatcat tgctgccatc atccacaagg atgatttcat
     9361 aggaatagtt ttgttcctca accacttttc tgatccaggc acacagttcc ggcaaagact
     9421 cttcttcgtt atataaaggt attacaattg acaaatccat aaaaaatgtt ttttacatga
     9481 cgacatcatt tattttttct tttttccgaa taaaaagtgc agcaattgca gccattacta
     9541 ttgagaatat agcattattc tgaagacttc ctgtcaattt gctgaccggg tttgcgccat
     9601 ccattatttt ttgtatatat acctctttgg tttcatcagg gatggacgca ttttcggaaa
     9661 ttatttccac cgctttctgc gcttcattta tcccccttgc aggatcaaag aacgcataaa
     9721 aaatataata atacaatgaa atcacaatgg ttgaaacaac agccatgacc aggcaaaaca
     9781 aaaatgcctg accataagta ataaaaccat tgcggtattt attacggtag tttttaccat
     9841 aataaatata caatccgata tagataccaa tggtaacaag cgaaatcagc agccctacca
     9901 tcattcccat atccgcaaaa acaaaaatat aggttagcaa atcaagcaga ataataacgc
     9961 cagccagcat caatccgcac cgtaatgcgt ttttccaaaa cgggctaccg aagcttttgg
    10021 gttgattggg cacctcaata atattttcag attccataat aactgatttt tttattagaa
    10081 caaaattaat attattttgt gcagtttaca gaattttttt tatttttgca acgggtaagt
    10141 cttatacgac cagctcccgc aaactccccc aggtccggaa ggcagcaagg gtaggcggtt
    10201 gtagcgg
//
LOCUS       DAOSFP010000027        10056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5700_length_10056_cov_3.567743, whole genome shotgun sequence.
ACCESSION   DAOSFP010000027 DAOSFP010000000
VERSION     DAOSFP010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10056
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5700_length_10056_cov_3.567743"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLI16_01570"
     CDS             <1..478
                     /locus_tag="PLI16_01570"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83279.1"
                     /translation="GTTLFNGTGPAVETPMNGPSANTLTLKIGPFNNPVQQVTQVALV
                     IHFDNNTWDNNNGQDFHIPINNNPTTAANNVAASKYVSIYPNPFEDYCFINISGSEWH
                     SYTLEIFDLQGHRVKSLLMQKNVQKVERDFLKAGLYIVKVTNMVTGEVFSDKVSVI"
     gene            609..2780
                     /locus_tag="PLI16_01575"
     CDS             609..2780
                     /locus_tag="PLI16_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83280.1"
                     /translation="MKQRILLLILLVLPLFSMAQQLYSSKNGIDIPPVIKMRMLNIFI
                     NINYDQTPERDPLLNNKEHPWQPGRPNSINTNVPVFLKSYMDPDLTHDKPQGMITKYY
                     YESSLGNFVCLGDFIMVDVAQSSITPQNPGVNFSAFDLINAAITVINKNGGVRTVFGH
                     DSVSDYDFFERGRGGLPKKTEPNGKIDFVQFVFRNSGRTFEGGKQLYNYGQNNPGEGN
                     YIPGGLGKNKLLKFGNEYYENEMTSFQNLGMTELLHMTKTIVIHEFAHSLLGGNDFHT
                     SGGNHYGTYGACPFIGLQGGWGLMGGYGASLISCNAYERWRLGWLSKQYNPHEFPISA
                     NEAHADITRDMGTQSFILRDFVTTGDAIRIRLPYIDTGSSNQYIWLENHQVGRNNKYD
                     YLSFSTTSACRDQGLPGIYAYLQVGRDILESEKFSDVYPSYDTDNLKVISAKGNWDRE
                     IVSMTDTVNCVAWRSVNHSESCSKPNPLCGYVDEQSHFFETQGDRITDKTFTDFPWIV
                     YRKGIRYDNLPYLGDNFDAFTDGATLDIGTNPSPANTLTCYVSQGGRKFTPYKRINTR
                     HIYLTGLSISMDDLKNGTFKVDIRWDDYTVKNNTRWTGDIILKEKLILQNGKTIQLDQ
                     NYTPGQLHRDSLSGYFAPLTTFTAEPSSEIILQKNSKLLIDNNSTLILEESSKLVLEK
                     KSQLILKNGARLIIRKGAEIQNKGGKIINKSSRKIINESEN"
     gene            2786..3307
                     /locus_tag="PLI16_01580"
     CDS             2786..3307
                     /locus_tag="PLI16_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOH83281.1"
                     /translation="MELPDYYKVLGIPHNAPPEEVKRAYKEKALKYHPDVYNGEFSVE
                     IFQLINMAYHTLIDPALRRQYDLRLKYPTYFANEKNTYKYRHAADARYYSRADHKPQA
                     APKHYARNIRRLNNFMLYSIVALLGFGIVMGAIDMIRTSDFTMLLASIACMIIIISGV
                     RIIKRSHYHKTKF"
     gene            3443..4708
                     /gene="mtaB"
                     /locus_tag="PLI16_01585"
     CDS             3443..4708
                     /gene="mtaB"
                     /locus_tag="PLI16_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOH83282.1"
                     /translation="MKTVAFHTLGCKLNFAESETIIRRLSENGYRIISFREKADVYVI
                     HSCIVTQQAEKKCIYALRQAMVRNPGARIAVMGCMSELNKARLQQEKSDLLLLGNTNK
                     YNLAEILEQQPAPDEISSATFVPAWSSTGRTRTFFKIQDGCDYFCGYCTIPFARGRSR
                     SATISDTMKSLDEAIASGCKELVLTGINIGDFGRKNQETLHQLIVEILKTQGIQRIRL
                     SSIEPNLISDDLIRLFAENQRLMPHFHIPLQAGNDETLLAMGRKYNTALFSEKVQKIK
                     EYMPHACIATDIIAGYPGETEKNHTDTLHYIEKTDLSYMHVFTYSERLLTRSAKNPEQ
                     VTPHIRKKRSLELHELSEQKKQCFYTQHIGRTCEALFESEKKNSDLLGYTDNYIRVKT
                     PMQKSLVNSIQKITITGIDSENVCTTKIH"
     gene            4735..5895
                     /locus_tag="PLI16_01590"
     CDS             4735..5895
                     /locus_tag="PLI16_01590"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH83283.1"
                     /translation="MFPQLSDIINYLFGTNINIPVQSYGMMIAVAFLTGIYFIKIELK
                     RKEKEGLLNVIVQKELKGAKASIPELIFLGITGFIIGYKLLGIIVDYKEFAENSQEYV
                     FSMKGNLFGAITLAIIMVVSRYLKKKKEKLDKPVWEEIQVHPYQLSANILIISAVAGL
                     LGAKIFHHLENFDAFLEDPAGQFFSFMGLTFYGGLIVGSLTVIWYIRKNKMPVPTFLD
                     ATAPAIILAYGIGRIGCMLAGDGCWGIENLNPKPEWMGFLPDWMWAFRFPHNVVNEGI
                     TLDNCTGKFCTILENPVYPTSFYDTVLCTLFFILLWSIRKKIKVHGWLFALTIGLIGI
                     QRFFLEKIRVNNKFNILGLQITQAEIISILLIILSITLFWFFYKNYKKKSKI"
     gene            6023..6799
                     /locus_tag="PLI16_01595"
     CDS             6023..6799
                     /locus_tag="PLI16_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH83284.1"
                     /translation="MTNRQWIFFSLIFSGIIFSSCSTYYQLQEEKTQASVIRVDTVNV
                     PYEDSTFVKLIAPYKEQLDAQMNEVLAYSDMVMKKSLPEGLLNNFVADLTLTMGNRYL
                     KKLGKDTASVCLLNSGGLRSDLPMGAVTLRNIYELMPFENEMVAVTINGSNAEKMFGF
                     IAHKGGMPMAGAIMGIGADTVSKIIIGNVAFDKSKNYTVITSDYLALGGDEMSFFASP
                     VSYHPLKLKMRDAIADYLREQTAKGKTITAQLDKRIYYEK"
     gene            6789..7691
                     /locus_tag="PLI16_01600"
     CDS             6789..7691
                     /locus_tag="PLI16_01600"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOH83285.1"
                     /translation="MKNKRTTFYIICILLFTVFTTPATGYGKDKEVKITILFTNDTHS
                     RIESYPANDSKNPGLGGYARRAALIKKIRSEEPNVMLFDAGDVFQGTPYFNFFGGELE
                     FKLMSMMGYTAMTLGNHDFDNGLEGLLKQLPQATFDIISSNYDFSATILKDKIQACKI
                     YNVAGVKVGVFGLGIELQGLVSALNYGKTRYLNPVEKAAEMAYLLKKEKKCDLVICLS
                     HLGFSYQDKKVSDATLAKQSKNIDIIIGGHTHTFMDKPYTFRNSDGKNIIIGQVGTGG
                     VKLGRIDLLFSKKNGIKVEHLSAL"
     gene            7872..9308
                     /gene="dnaA"
                     /locus_tag="PLI16_01605"
     CDS             7872..9308
                     /gene="dnaA"
                     /locus_tag="PLI16_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOH83286.1"
                     /translation="MKKSHTEVWNNCLNIIRDNISYQSFKTWFEPITPIKLVDSVLTI
                     QVPSQFFYEWLEEHFIELLRKTISKELGPEGQLEYSIVMENSYGNDTPVTVKAPGVIR
                     KPTKNPTVSMPIDINKGSSKDIPNPFIIPGLKKIQIPSQLNENYSFENFIEGDCNRLA
                     RSAGYAVANNPGKTAFNPLLLYCNNGLGKTHLCHAIGLQIKQNFPDKIVLYVQCEQFI
                     TQYIEAVKTGNQNDFMHFYQMIDVLIMDDIEFLSAKEGTQNAFFHIFNHLHQQGSQIV
                     ITSDKAPIDLKGIEPRLLSRFKWGLAADLQVPDFETRVAIIDKKLYNNGIEMPRDVIE
                     YLAYSITTNIRELEGALVSLLAQSSLNKKAITIDLAKQIIDKFIRNNSREISIDYIQK
                     VVCDYFGINIDLLNSKTRKREIVQARQLSMFFAKKLTKASLASIGMHCGKKDHATVLH
                     ACRTVNNLIETDKQFKLYVDELEKKIKL"
     gene            9523..9972
                     /locus_tag="PLI16_01610"
     CDS             9523..9972
                     /locus_tag="PLI16_01610"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH83287.1"
                     /translation="MIRLLTPDDFDAWMALAAEVEPLFGPMTTMPAFKEAISNCVKYK
                     NAYGISFKNEILAGIVAVDREKNEIVWLAVSQKYQGKKLGDKLVKKCIEEMADRGDIF
                     VQTFAENIAAGIPARRIYERNGFTDLMKAGKNPAGIDTVIMVKRKKT"
BASE COUNT         3253 a         2270 c         1994 g         2539 t
ORIGIN      
        1 aggcactacc ctgtttaacg gcaccggccc cgcagtggaa actccgatga acggtccgtc
       61 agccaacacc ttaaccctga aaataggacc ttttaacaac ccggttcagc aagttaccca
      121 ggtggcgtta gtcatacatt tcgacaacaa cacctgggac aacaacaacg gccaggactt
      181 tcatattcct ataaataata atcccaccac tgcagcaaat aatgtggctg ccagcaaata
      241 cgtcagcatc tatcccaacc cttttgaaga ctattgtttc atcaatattt caggcagcga
      301 atggcactcc tacacactgg aaatatttga ccttcagggg cacagggtaa aatcgctgtt
      361 gatgcagaaa aatgttcaaa aagtggagcg tgattttctg aaagcaggtt tgtatattgt
      421 gaaagtaaca aatatggtta caggagaagt tttcagcgat aaagtcagcg ttatctgaaa
      481 acagaaaagt aaataaaaat acctaccttt gttattaagc aaagagagta gtgttgtaag
      541 agaattaccg ttcgaacaat tacactttct atgtaaagga aatttattaa tagaattaat
      601 atatcctctt gaaacaacgc atactgcttc tgattttgtt agtcctgcca ttattcagca
      661 tggcacagca actttattcc agcaaaaatg gtatcgatat ccctcctgtt ataaaaatgc
      721 ggatgctgaa tatatttatc aacatcaatt acgatcaaac cccggagcgc gatcccctgc
      781 tgaacaacaa ggaacacccc tggcaacctg gcagacccaa tagtatcaac acaaatgtgc
      841 ctgttttcct gaaaagctat atggaccccg atctgacaca tgataagccg caaggcatga
      901 taaccaaata ctactatgag tcaagcctgg gaaactttgt atgcctggga gatttcatca
      961 tggtggacgt tgcccagtcg tcaataactc cgcaaaaccc tggagtcaac ttttcggcct
     1021 tcgacctgat aaatgccgct atcacggtga taaataaaaa tggaggcgtc agaacagtct
     1081 tcgggcatga ttcggtcagc gattacgact tttttgaacg cggccgcggc ggattgccaa
     1141 aaaaaacaga acctaacggc aaaatcgatt tcgtgcaatt tgtatttcgc aattcaggca
     1201 gaacatttga aggaggaaaa caactttaca actacggcca aaacaacccg ggagagggca
     1261 attatatacc gggcgggctg ggaaaaaaca aattattgaa atttggcaat gaatattacg
     1321 aaaacgaaat gacctcgttt caaaacctcg gcatgacaga actattgcac atgaccaaaa
     1381 ctattgtaat tcacgaattt gctcactcgc tgctcggagg aaatgatttt cacacatcag
     1441 gaggaaacca ctacggcacc tatggcgcct gccctttcat cggattacag ggaggctggg
     1501 gcctgatggg cggatacgga gccagcctga ttagctgcaa tgcttatgag cggtggcgtt
     1561 taggatggtt gagcaaacaa tacaaccctc atgagttccc tatttcggct aacgaagccc
     1621 acgctgatat tacccgtgac atgggaacac aatcattcat cctccgcgat tttgtgacaa
     1681 ctggcgatgc catccgtatc aggctcccat acattgatac gggttcatca aaccagtata
     1741 tctggctcga aaatcaccag gtaggccgca acaataaata cgattacctt agtttctcca
     1801 cgacatcggc atgccgtgac cagggtctcc cgggaatcta tgcctatctg caggtgggca
     1861 gagatatcct ggaaagcgaa aaattttccg atgtgtaccc ttcttacgac accgacaacc
     1921 tgaaggtaat cagcgcaaaa ggaaattggg accgtgaaat agtaagcatg accgacacgg
     1981 tgaattgtgt ggcctggcgt tcggtaaacc attccgaatc gtgctccaaa ccaaacccac
     2041 tgtgtggtta tgttgacgaa caaagtcatt tttttgagac acagggtgac cgtattaccg
     2101 acaaaacctt tacggatttt ccatggatag tgtacagaaa aggcatcaga tacgacaacc
     2161 tcccatacct aggcgacaat tttgatgcgt ttaccgatgg cgccaccctg gatatcggca
     2221 ccaatccttc gcctgcaaat acccttacct gttatgtttc gcaaggcggc agaaaattca
     2281 caccatataa aagaatcaat acgcgtcata tttatctgac cggcctttcc atttcaatgg
     2341 atgacctgaa aaacggcacc tttaaagtgg acatcaggtg ggacgactat accgttaaaa
     2401 acaacacccg ctggaccggc gacatcatac tgaaagaaaa actcatcctg caaaacggca
     2461 aaaccataca actcgatcag aattatactc caggccaact gcatcgcgac agcctttcag
     2521 gttattttgc acccctgaca acattcactg cagagccgtc cagcgagatt atcctacaga
     2581 aaaattcaaa attactaatt gacaataaca gcaccctgat actggaagaa tccagtaaac
     2641 tggtgttgga aaaaaaatca caactgattc ttaaaaacgg tgcacggttg atcattagaa
     2701 aaggggcgga aatacaaaat aaaggcggca aaattattaa taaaagctcc cggaaaataa
     2761 ttaatgaatc tgaaaactaa aatttatgga acttcccgat tattataaag tgcttgggat
     2821 tccgcacaat gcgccgcctg aagaagtaaa aagagcttac aaggaaaaag cccttaaata
     2881 ccatccggat gtatacaacg gagaattctc agtagagatt tttcagttaa taaatatggc
     2941 ctatcacaca cttatcgacc ctgccctgcg cagacaatac gatctgcgac tgaaatatcc
     3001 aacgtatttt gccaacgaaa aaaacacata taaataccgg catgcggcgg atgcacgtta
     3061 ttacagccgt gcagatcata aaccacaggc agctccgaag cattatgccc gaaacatccg
     3121 aagattaaac aacttcatgc tctattccat tgtggcactc ctgggttttg gaattgtaat
     3181 gggagccatt gacatgatac ggacttcaga ctttacgatg ttgctggcca gtatagcatg
     3241 catgataatt attatttccg gagtaaggat tataaaacga tcacattacc acaaaacaaa
     3301 gttctgaaaa tcagctttat tccacgcaaa caacagttgc ccgctctttg gtctcaggct
     3361 taatcctcac tgcaaattct gaaaaattta caaaacaagg ccacgccttt cggccgaaaa
     3421 ttgtatcttt gccctcttgt tgatgaaaac tgttgccttc catacactgg gctgcaagct
     3481 caattttgcc gaatcggaaa caattattcg ccggctctcc gaaaacggat atcgcatcat
     3541 cagcttcaga gaaaaagctg atgtttatgt gatacactct tgtattgtaa cacaacaagc
     3601 tgaaaaaaaa tgcatctatg ccttacggca ggcaatggtg cgtaatcccg gcgcccggat
     3661 tgccgtcatg ggctgcatgt cggaactgaa caaagcaagg ttgcagcagg aaaaatccga
     3721 cctgttgctg ctggggaaca ccaacaaata taatcttgcc gagatactgg aacagcagcc
     3781 ggctccagac gaaatcagct ccgcgacctt tgtccctgca tggtcttcca caggcagaac
     3841 ccgaaccttt ttcaagatac aggacggttg tgattacttt tgtggttatt gtaccatacc
     3901 ttttgccaga ggacgaagca gaagcgccac catcagcgac accatgaaat ccctggatga
     3961 agccattgcc tcaggatgta aggagctggt gctcaccgga attaacattg gtgacttcgg
     4021 cagaaaaaac caggagaccc ttcaccaact tattgttgaa atcctcaaaa cacaaggcat
     4081 acagcgtatc cgcctctcct ccatagaacc gaatcttatt tcagatgacc ttatcaggct
     4141 gtttgctgaa aaccaacgcc tgatgcccca ttttcacatc ccattgcagg caggaaacga
     4201 tgaaacgctc cttgctatgg gaagaaaata caacaccgcc ttattttctg aaaaagtgca
     4261 aaaaattaaa gaatatatgc ctcatgcctg catagcaaca gatattatcg caggataccc
     4321 cggcgaaaca gaaaaaaacc ataccgatac attacattat attgaaaaga ctgatctttc
     4381 atacatgcat gtctttacct attcagagcg actgctgacg cgttcggcaa aaaatcctga
     4441 acaggtaact ccccatatcc ggaaaaaacg cagccttgaa ctgcacgaac tttccgaaca
     4501 aaaaaagcaa tgtttctata cccaacatat tggtagaacc tgcgaggcgc tctttgaatc
     4561 ggagaaaaaa aacagcgacc tgctgggata tactgataat tatatccggg taaaaactcc
     4621 tatgcaaaaa tcgctggtca attcgataca aaaaattaca attaccggca ttgattctga
     4681 gaatgtctgt accaccaaaa ttcattaaat caatcttttt aacttcaaca tacgatgttt
     4741 cctcaactta gcgatattat taattatttg tttggcacca acatcaatat acccgtccaa
     4801 agttacggaa tgatgattgc cgtagcattc cttacaggaa tttatttcat aaaaattgaa
     4861 ctgaaaagaa aagagaaaga aggcttactg aatgtcattg tgcaaaaaga actcaaaggc
     4921 gccaaggcaa gtattcctga actgattttt ttaggaataa ccggatttat tatcggttat
     4981 aaattactgg ggataatcgt tgactacaaa gagtttgcag aaaattcgca ggaatatgtt
     5041 ttttcgatga agggcaactt attcggagcc ataaccctgg caatcattat ggtagtcagc
     5101 agatacctca aaaaaaagaa agaaaagctc gacaagccgg tgtgggagga gattcaggta
     5161 cacccctacc agctttcggc caacattctt atcatctctg ctgttgcagg cctgctgggc
     5221 gctaagattt ttcatcatct tgaaaacttc gatgcatttc tagaagatcc tgccggacag
     5281 tttttttctt tcatgggtct cactttttat ggtgggttga ttgtgggcag cctgacggtg
     5341 atatggtaca tccgaaaaaa caaaatgcct gttcccacat ttctcgacgc aacggctcct
     5401 gccataatac tggcctacgg tattggccgt ataggatgta tgctcgcggg cgacggttgc
     5461 tggggtattg aaaatctcaa ccccaagccc gaatggatgg gatttctgcc cgactggatg
     5521 tgggctttcc gtttcccgca caacgtcgtt aacgaaggaa ttaccctgga caactgcacc
     5581 ggaaaattct gcaccatact tgaaaacccc gtttacccca cttcctttta tgataccgtg
     5641 ttatgtactt tgttcttcat attgttatgg agcataagaa aaaaaatcaa ggtgcatgga
     5701 tggcttttcg ctttgacaat tggtctcatc ggaatacaaa gattttttct tgaaaaaatc
     5761 cgggtaaaca acaaattcaa tattttgggt ttacaaatta cccaggcaga aattatttcc
     5821 atcctgctca tcatccttag tatcactttg ttttggtttt tttacaaaaa ctacaaaaaa
     5881 aaatctaaga tttgattttg aaaatcgaaa aattttcaag tactttgtaa gcctttttga
     5941 gtaagccacc cgcgctgcag gtattgccaa ataaggaatt cagacaagct ataaaaaact
     6001 tcgcacctgt aaaaacaaaa caatgacaaa caggcaatgg atatttttct ctctgatttt
     6061 ttccgggata attttctcct cctgttcaac ttattatcaa ttgcaggaag aaaaaactca
     6121 ggcaagtgtc atcagggtag atactgttaa tgtcccatac gaagacagca ctttcgtaaa
     6181 gcttatcgca ccatataaag aacaactgga tgcccaaatg aatgaagtgc tggcctactc
     6241 agacatggta atgaaaaaaa gcctgcctga aggcttgctg aataactttg ttgctgacct
     6301 cacactgacc atgggcaaca ggtacctgaa aaaactcgga aaagacaccg cttccgtctg
     6361 cctgctgaat tcgggcggcc ttcggagcga tctgcctatg ggtgcagtta cgttgcgtaa
     6421 tatatatgaa ctaatgccct tcgaaaacga aatggtagca gtaaccatca acggttccaa
     6481 cgcagaaaaa atgttcggat tcatcgcaca caaaggaggc atgccgatgg ccggtgccat
     6541 catgggcata ggcgccgaca ccgtgagcaa aattataatc ggcaatgtag ctttcgacaa
     6601 atcaaaaaac tatacggtaa taacatccga ctatctggcc ttaggtggcg acgaaatgtc
     6661 tttttttgca tcgccggtgt cctaccatcc attgaaatta aaaatgagag acgctatagc
     6721 cgattacctc agggagcaaa cagccaaagg aaaaaccatc acggcacaac tcgacaaaag
     6781 gatatattat gaaaaataaa cggacaacct tttatataat atgtatcctg ctatttacgg
     6841 tgtttaccac ccctgctacc ggttatggta aagacaaaga ggttaaaatc actattcttt
     6901 ttaccaacga cacgcatagc cgcatagaat cttatcctgc caacgacagt aagaatcccg
     6961 gacttggtgg ctatgcacga agggctgccc tgataaaaaa aatacgaagc gaagaaccca
     7021 atgtcatgct ttttgacgca ggagatgtgt ttcaggggac cccctatttt aatttcttcg
     7081 gtggcgaact tgaatttaaa ctcatgagta tgatgggtta caccgccatg acactcggca
     7141 accacgactt cgacaacggc cttgaaggct tgcttaaaca actgcctcag gcaacttttg
     7201 atatcatctc ttcaaactac gacttctcgg caactatcct gaaagataaa atacaagcct
     7261 gcaaaatcta taatgtggcc ggagtgaaag ttggcgtatt cggcctcggt attgaactac
     7321 agggattggt aagcgcactg aactacggca aaacccgcta cctgaacccc gtggaaaaag
     7381 ctgccgaaat ggcctatctg ctgaaaaaag aaaaaaaatg cgatctggtc atttgcttat
     7441 cgcatctggg tttttcttat caggacaaaa aagtaagcga tgcaaccctg gccaaacaat
     7501 cgaaaaacat cgacattatc attggaggac atacccatac ttttatggac aaaccctata
     7561 ctttcagaaa ctcagacggc aaaaatatta ttatcggtca ggtgggcacg ggaggagtga
     7621 aactgggaag aattgattta ttattttcta aaaaaaatgg tataaaagtt gagcatttga
     7681 gcgctcttta agaaaaagaa aaaaaaatga aaaaaaactt ttttgctatc attattttta
     7741 taatatttgt aaagttgttc tgataatcat ttatttttat ctgaatcagg ttagttcctt
     7801 ataaaagaat aggttaataa aaaaacaaaa agaaaacatt tttaaattcg aagaaattga
     7861 aggagcaaag gatgaaaaaa agccataccg aagtttggaa caactgtctg aatattatcc
     7921 gcgataatat ttcttatcag agtttcaaaa catggtttga accaataacc cccataaaac
     7981 ttgtcgattc ggtactcact atacaggttc ccagccagtt tttttatgag tggctcgaag
     8041 agcacttcat tgaattgttg cgcaaaacca tttccaaaga actcggcccc gaagggcaac
     8101 tggaatacag catagtgatg gaaaacagtt acggcaatga cacacctgtt acagtcaaag
     8161 cacccggcgt aatccgtaaa cccaccaaaa atccaacggt ttccatgccg attgacatca
     8221 acaaaggcag ttctaaagat atccccaatc cctttattat tccgggatta aagaaaatcc
     8281 agatcccttc tcaactgaat gaaaattact cttttgaaaa ttttattgaa ggagactgca
     8341 accgcctggc ccgttccgcc ggctatgccg tagccaacaa ccctggaaaa actgcattca
     8401 atcccctcct gctctattgc aacaacggcc ttggtaaaac ccacctgtgt catgctatcg
     8461 ggctgcagat aaaacaaaat ttccccgaca aaatagtact gtatgtacag tgcgaacaat
     8521 ttatcaccca atacattgaa gctgtaaaaa ccggcaacca gaatgatttt atgcacttct
     8581 accagatgat agacgtgctc atcatggatg atatagagtt tttgtcggcc aaagaaggaa
     8641 ctcaaaatgc cttcttccat atcttcaacc acctgcatca gcagggaagc caaatagtaa
     8701 taacttccga caaagcccca atcgacctca aaggcattga accccgcctg ttatcccgct
     8761 tcaaatgggg acttgctgca gacctgcagg taccagattt tgaaacccgc gtggccatca
     8821 ttgataaaaa attgtataac aacggtatcg aaatgccacg cgatgttatc gaatacctcg
     8881 cttacagcat caccaccaat atacgcgaac tcgaaggcgc tttggtatct ttgctggcgc
     8941 aatcctccct caacaaaaaa gccatcacca tcgatctcgc caaacagatt atcgacaaat
     9001 ttatccgtaa caattcgcgc gaaatttcaa tcgattatat tcagaaagtg gtatgcgatt
     9061 atttcggcat aaacatcgat ctgctgaact ccaaaacccg caaacgtgaa atcgttcagg
     9121 ccaggcaact gtcaatgttc ttcgcaaaaa aacttaccaa agcttcctta gcatctattg
     9181 gtatgcattg cggaaaaaaa gaccacgcca cagtgttgca tgcctgccgc acagtgaaca
     9241 acctgataga aaccgacaaa cagtttaaac tgtatgttga cgaattagaa aagaaaatta
     9301 aactttaaga gctacgcctt ttcttactcc aactcataac actgtcacct attgacaaca
     9361 aggatttatt aatcctatgg tggtgcaggt gcacacagta caaacaggaa tggatttatc
     9421 aaaaaactca ttgaggacac aacaaattcc cgcaaaggca aaatcgtgct cgtaccgtta
     9481 ggttggtcaa aaattttact tacgtaacaa agaattatta gtatgatcag gttattaacc
     9541 ccagatgatt ttgatgcctg gatggctctg gctgccgaag tggagccgct tttcggtccg
     9601 atgaccacca tgccggcatt taaagaagca atcagcaatt gtgttaaata taaaaatgcc
     9661 tacggcatca gttttaaaaa tgaaattttg gcaggcattg ttgcggttga ccgtgagaaa
     9721 aacgaaatcg tgtggctggc cgtttcacaa aaatatcagg gaaaaaaatt aggcgacaaa
     9781 cttgtgaaaa aatgcataga agaaatggca gaccggggag atatttttgt ccagactttt
     9841 gccgaaaata ttgcggccgg aataccggcg cgacgtattt atgaacgcaa cggctttacc
     9901 gatcttatga aggcagggaa aaatccggca ggcattgata cggtaatcat ggttaaaaga
     9961 aaaaaaacgt aaaacatagc ctaagctatt caaagtagca tcagcatgcc atttacctcc
    10021 tgattaaaaa actggatgtt ttttcccaac ctttcg
//
LOCUS       DAOSFP010000028        10044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5713_length_10044_cov_3.953048, whole genome shotgun sequence.
ACCESSION   DAOSFP010000028 DAOSFP010000000
VERSION     DAOSFP010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_10044_cov_3.953048"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PLI16_01615"
     CDS             complement(<1..1120)
                     /locus_tag="PLI16_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOH83288.1"
                     /translation="MMETEIKNKNYLGINSLGRIGKLSLWNHLVTRHFDGVVINIGRK
                     TGKNIEAMIQALENDSTYGNLGNFLYGQKSRKDVIKVIDSEKFIFDIDGMMVKVLTEE
                     RDPAKIPWKEEQVQLVLDCTGKFVDPTVPIDSPKGSLRGHLAGGARKVIVSAPFKIKD
                     AEKQTPTDSIMMVYGINHIEYDPQKHDVISAASCTTTGLSHMIKPLMEDRYTSKILTA
                     SMSTVHAATNTQSVLDSLPKAGASDLRKTRSVFNNIILSTTGAAKALESIIPEIQEIG
                     FMADSVRIPTTTVSLITLNITFNSGLDEKGNPIINRQHINNIYKTAAAGSQKGLLVFS
                     DRQNVSADLTGYKAAVVIEGSENHTRTGFIKLPAETVKS"
     gene            complement(1173..5255)
                     /locus_tag="PLI16_01620"
     CDS             complement(1173..5255)
                     /locus_tag="PLI16_01620"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH83289.1"
                     /translation="MNRPIKSTGLAANLKITQLPKDIVLREEHVWLLGLSESHYGIHE
                     RTKNFFDEYYHGYINWDHVSDELRRLALGDFWFYTQCPDQAKALDIILQLFEESLEKT
                     VQTKAFEMVFMAMIEFLAMITKLETVQMQLESKGLDIIKRWGEHNEVSVLRNATFIKN
                     QLSVLNNNPVLKDKFFEVIHLLAGRVISYWAENSWAETWYEKNKDLFSEDFADELSFI
                     GKPYFDDLMEKWRQVKTFEELDKNIPLYSEITKYFIGRTDTFQIIANRFYFLVYLLHI
                     PAMAHVTNTLLINLNILIKRILEKIEPEQVKPFIDNIFKLFHELQKEHSSTVLECIST
                     LGKEIVNSSKVENTKDFVNKIIELPFVTPGPLHMSKEWRTEVDPSHIKNIRVWLSLFE
                     TKPELYHKLILALTVNLKLKGIFIFDTDLFQKDISALLNSDFHPYFRTVKQLCRIFPV
                     YFNEIGAEGELRDITTAIDEMTHRDDKLIHFLRKLVHIESNNTHISFTKKVFNYWIDG
                     NLKPLEPFLPEDVKAAIDPKWAIPMQKLILKTAEKAHIKPSQLIEEHPDKVDKWVDSV
                     SDHSQINKKRLKMLCRIYFILIEKYSFNTVNLGKSIRKYNFIAENEIELLEEAIQKKN
                     REDALKLIFHFISMLKEIILSKEKTEGWENIYHKRHVAFGIPSMYGDYHERRFEAGGL
                     IFKLEQAAHLIMEEWVKSINLNYITAKILVKIDSILAIYKEGLELDGITNDVFISNMG
                     MFSNSLKSQSFSLHQYINIFEFMSENIREIISKYFFSPYEDILKIVIPRMREGHPEPG
                     PSDNSFFHMQSETFYRDMLSSSFLLQSLDNFITEILTSLRNMVETLSAERIRDIMSYN
                     PDMVISPLYGEATVVDNQVFIGSKAYFLKKLKALQYPVPPGFVITTEVFRRKNTILQH
                     KMLSREIDTMIQAQIRRLEKETRLELGNPKKPLMLSVRSGTAVSMPGAMNTFLNIGIN
                     EEIIEELSKQPNYGWTSWDCYRRFLQSWGMASGISRDTFDQIMMDFKKADKVDLKVQF
                     KPHRMRDMAFAYREILDKNNIHIEEDMFLQVRQAILSVFDSWNSSRAKVYRHHLHIAD
                     EWGTAVIVQKMVLGNINYTSGTGVFFTNNPHKREPGIHLYGDYSLVSQGEDVVGGLVH
                     VLPISQDQPHVHGQEKSLQEAFPRLYERLYNIAKELVEVHGFSHQEIEFTFESDDPND
                     LYILQTRDQDTRPKNKKQMFAISDENMVLLGKGVGIGGGAINGILVFDDKGLREMRQE
                     QPDKKLILVRPDTVPDDIDLIFRCDGLLTSRGGATSHAAVTAARLGKICIVNCTDLVV
                     NEREGICNIKGHTLAAGDKIAIDGNLGNIYLGHYHMMINE"
     gene            complement(5444..6787)
                     /locus_tag="PLI16_01625"
     CDS             complement(5444..6787)
                     /locus_tag="PLI16_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOH83290.1"
                     /translation="MCVWTALLVATTQASAQSFTSKSSGIIRVYFTNTVDNSISPYAD
                     GMYNPHMEDTIVAYINKAHHTLDMCVYDNRSDAIISAVNQAYIRGVTVRFISETTALN
                     YSLAGLNPSVALLKRNDGTNIMHNKFVIIDRDEENNAWVITGSANHTPDNLTIDPNNV
                     VFIQDKQLALAYTTEFEEMWGSATATPNLSNSKFGDAKTDNTPHTFTIGGKTVELYFS
                     PSDGTSTHIETAVNTTDSDFCFAMFTFIHNGIGDAVEDVHNSGVAVKGIIENVSYFGS
                     EYNSLLSAGIPVLSHENVPNSFHHKYGVADATSPSSDPLVVTGSHNWTNSAEEDYDEN
                     TLIIHDGQIAAMYFEEFMTRFNELSLGTPETISEDGIFYFDPAKRSLIICGKHDGSEN
                     LVDLFDLTGRTIFKKKIPNINESPLEITLPEDMPGLFIAKISSRCHTGIIKIFLP"
     gene            complement(6856..8322)
                     /locus_tag="PLI16_01630"
     CDS             complement(6856..8322)
                     /locus_tag="PLI16_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOH83291.1"
                     /translation="MIFYKKSLLSLFSSLFFAFSALSFPFSSSLPQHHPDSSFYKDNP
                     VVAQFDSLATLAFFTNSTFTTEVSKLNIYNFPADSVPVYDDSVYSKRINIMSINSPFE
                     YVYNQQVRNYIDVYSVQKRKLTSRMLGLAEIYFPLFEEQLDNYDLPLELKYLAIIESA
                     LNPIARSRCGASGLWQFMLTTGKMYDLQVSSYVDDRFDPYKSTVAACRHLKDLFNIYQ
                     DWAIVLAAYNAGAGCINKAIRIANLDSSERITYWKIQRFLPVETQNYVPAFIGASYVM
                     TYAAEHNLYPTQPPILHCEIDTVTVTKNYNIGQISSYLCMPAEEIKFLNPAYKKGIIP
                     ATEEKPYVLCLPRKIMGEFISNEDAIYCYKSPQEAAYQQQLKLMSASKTKPSPVMAKA
                     SVSKTQAPAPTTTQAAEQTTTTAPAQAQAAIQAKPASPKTTASEKYIYHTVQKGDTLW
                     GIAEKYNGVTVEDIRRLNNLNSKNLIQPGQKLKINLKG"
     gene            complement(8457..9170)
                     /locus_tag="PLI16_01635"
     CDS             complement(8457..9170)
                     /locus_tag="PLI16_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOH83292.1"
                     /translation="MKCFFSENFKYLSARRKTDAVQWAKQTGISESLIRDFLKKKALP
                     DAEQLIKISETLNYPIAQFLTVNIQARERLVQTFDFKFLVVDIDGVMTDGGIIYTENG
                     DEIKKFHARDGLAIISLINAGYQVGFLSSGFTQNTITHRAKILGVKKVYIGTWKKLEV
                     LEKWCNEMKITMQNVAYIGDDLNDIPVIEKVGLSACPADAAEAVKEKVTIILSKCGGQ
                     ACVREFADNYLSTLYKTPF"
     gene            complement(9167..>10044)
                     /gene="aroF"
                     /locus_tag="PLI16_01640"
     CDS             complement(9167..>10044)
                     /gene="aroF"
                     /locus_tag="PLI16_01640"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOH83293.1"
                     /translation="KTEEADKDFFTSSFVFDTDIQLASKKYIPEKRTIRIGENYIGGN
                     TNNVFIAIGPCSVESENQITQSAEFIKKCGLKSMRGGCFKPRTSPYTFQGLGVKGLEL
                     LDKMRRLYGYTIITEVRDSSHVDDVIEYADIIQIGAKSMYDHGILRKCGKTQKPVLLK
                     RGFGTTLQEFIQAAEFILSGGNPNVMLCERGIRTFETKTRFTLDLCGAAYLKENTNLP
                     VILDPSHAMGYAYGVPDLARACMAMGVDGLLIEVHPNPSVALSDASQQLTHQQFEALF
                     ETLKPIAKAINRNII"
BASE COUNT         2571 a         2135 c         2269 g         3069 t
ORIGIN      
        1 acgattttac ggtttcggcg ggcagtttga taaagccggt gcgggtgtgg ttttccgaac
       61 cttcaatgac tacggctgcc ttgtatccgg taaggtctgc cgacacattc tgccggtccg
      121 aaaataccaa caggcctttt tgtgaaccgg cagcggcagt tttgtaaata ttgttgatgt
      181 gctggcggtt gattatagga tttccttttt cgtcaagacc ggaattaaaa gtgatattca
      241 gggtaatcag cgatacggtc gtggttggaa tacgcaccga gtcggccatg aagcctatct
      301 cctgtatttc ggggatgata gattcgagcg ctttggcagc gccggtggtg gataatatga
      361 tgttgttgaa caccgaacgt gtcttgcgga ggtcgctggc accggctttg ggcagcgagt
      421 cgagcaccga ctgggtgttg gtagccgcgt gaacggttga cattgatgcc gtgagtatct
      481 tggaggtata gcggtcttcc atcagcggct tgatcatgtg cgacaagccc gtggtggtgc
      541 aactggcagc agaaataaca tcatgttttt gagggtcata ttcaatatgg ttgatgccgt
      601 aaaccatcat gatactgtcg gtcggagttt gtttttcagc atccttgatt ttaaaaggag
      661 cgctgacaat caccttgcgt gccccgccgg ccagatggcc cctcaggctt cctttgggag
      721 agtctatggg cacagtggga tccacaaatt ttccggtgca gtcgagcacc agctgaacct
      781 gttcttcttt ccatggaatt ttggcggggt cgcgttcttc ggtaagcact ttcaccatca
      841 tgccgtcaat gtcgaaaatg aatttttcgg aatcaataac cttgatcaca tcctttcttg
      901 acttttgtcc atagagaaaa ttgccaaggt tgccgtaggt ggaatcattt tcgagagcct
      961 gtatcatggc ttcgatgttt tttccggttt tacggccaat gttgataaca acgccatcaa
     1021 aatgccttgt taccaaatgg ttccacaagg atagtttccc gattctgccc aggctgttga
     1081 ttcccaggta gtttttgttt tttatttctg tttccatcat aaaaataatt aggtgttaaa
     1141 atttattagg atgattattt tttttcaaca gtttattcat ttatcatcat gtggtaatga
     1201 ccaaggtaaa tatttcccag atttccgtca attgctattt tgtcacccgc tgccagtgtg
     1261 tgtcctttga tgttgcagat accttctctt tcgttaacca ccaggtcggt acagttcaca
     1321 atgcatattt tacccagtcg cgctgcagta actgcggcgt gcgaggttgc ccctcctctt
     1381 gaggtgagca ggccgtcgca gcggaaaatc aggtcgatgt cgtcaggcac cgtatccggc
     1441 cttacgagga tgagtttttt gtctggctgc tcctgcctca tttctctcag ccctttatca
     1501 tcgaacacca gtattccgtt tatggcaccg ccgccaatgc ctactccctt tcccagcagt
     1561 accatattct cgtctgaaat ggcaaacatt tgttttttgt ttttcggccg ggtgtcctgg
     1621 tcgcgggttt gcaatatgta aaggtcgttg ggatcgtccg attcaaaggt aaattctatt
     1681 tcctggtggc tgaatccgtg tacttccacc agctctttgg cgatgttgta taaacgttca
     1741 tacaagcgtg gaaatgcttc ctgcagcgat ttttcctgcc catgtacatg tggctggtct
     1801 tgcgatatgg gaagcacgtg taccagcccg ccgaccacat cttcgccctg gctcaccagc
     1861 gaatagtctc cgtacagatg gatgcccggt tcccttttgt gtgggttgtt ggtaaagaat
     1921 actcccgtgc ccgaagtgta attgatattg cccagcacca ttttttgtac aatcaccgcg
     1981 gtgccccatt catcggcaat gtgcagatga tggcggtaaa ctttagcccg cgaagaattc
     2041 catgagtcaa aaacacttaa tatggcctgc ctcacctgca aaaacatgtc ttcttctatg
     2101 tggatgttgt ttttgtcaag aatctcccgg taagcaaatg ccatatcccg catacggtgc
     2161 ggcttaaatt gcactttcag gtccacctta tcggctttct taaaatccat catgatctgg
     2221 tcaaaggtat cgcgcgaaat acccgaagcc atgccccagc tttgcaaaaa cctccggtag
     2281 caatcccacg aggtccatcc gtaattgggt tgtttgctca gttcttcaat aatttcttcg
     2341 ttgataccga tattcagaaa ggtgttcatg gcccccggca tggatacggc ggtacccgat
     2401 cgcactgaca gcatcaatgg tttttttgga ttcccgagtt ccagcctggt ttctttttcc
     2461 agccgccgta tctgggcctg aatcatagta tcaatttccc tgctcagcat tttatgctgt
     2521 aaaatggtgt tttttcgcct gaagacctct gtagttataa caaaaccggg cggtacagga
     2581 tattgcaggg ctttgagttt ttttaaaaaa taagcctttg acccgatgaa gacctgattg
     2641 tccaccaccg tggcctcgcc atataaggga ctgattacca tgtcggggtt gtacgacatg
     2701 atgtcccgga ttcgttctgc gctcagggtt tccaccatgt ttctcaacga agtcagaatt
     2761 tctgtaatga aattatctaa actttgcaac agaaatgaag aggataacat gtcccggtaa
     2821 aaagtttccg attgcatatg gaaaaagctg ttgtcgctgg ggcctggttc agggtgtcct
     2881 tcgcgcattc gcgggatcac gattttcagg atgtcttcgt atggactgaa aaagtattta
     2941 ctaatgattt cacggatgtt ttcgctcatg aactcaaaaa tattgatata ctgatgcagc
     3001 gaaaaactct gcgatttcag gctgttgctg aacatgccca tgttggagat aaacacatcg
     3061 ttggtaatgc cgtcgagttc cagcccttcc ttgtaaatag ccaggatgga gtcgattttt
     3121 acaagtatct tggctgtgat ataattcagg ttgatggatt ttacccattc ttccataatc
     3181 aggtgggctg cctgttccag tttaaaaatg agtcctcctg cctcaaaacg gcgttcatgg
     3241 taatcgccgt acatcgaggg tattccaaag gccacatgcc ttttgtgata gatgttttcc
     3301 caaccctcgg ttttttcttt gctcaggatg atttctttga gcattgaaat aaaatggaaa
     3361 attagtttca gcgcatcttc acggtttttc ttctgaatag cttcttctaa caattcaatt
     3421 tcattttcgg cgataaaatt gtatttacgg atactttttc ccaggtttac cgtgttgaat
     3481 gaatattttt ctatcaggat aaaatatatc ctgcaaagca ttttcagcct ttttttgttt
     3541 atctgggaat gatcgctcac ggaatctacc catttgtcca ccttgtcggg gtgttcttca
     3601 ataagttgcg acggtttgat atgtgctttt tctgcagtct tcaggatgag tttttgcatg
     3661 ggaatagccc atttagggtc gatggcggct ttcacatcct caggcaaaaa aggctcaagc
     3721 ggtttcaggt tgccgtctat ccagtagttg aagacttttt tggtaaaaga gatatgggta
     3781 ttgttgcttt cgatatgaac gagcttgcgc aggaagtgta tcagcttgtc atcgcggtgt
     3841 gtcatttcat caatggccgt ggtgatatcg cgcaattcgc cttctgcacc gatctcgttg
     3901 aaatataccg ggaaaattct gcacaattgc ttgaccgttc tgaaatacgg atgaaaatca
     3961 gaattcagca gggctgagat gtctttctga aacaggtcgg tatcaaaaat aaatatacct
     4021 ttcagtttca ggtttaccgt gagcgccagg attagtttgt gataaagctc aggcttggtt
     4081 tcgaacagcg aaagccatac gcgtatgttt ttgatatggc ttggatccac ttcggtgcgc
     4141 cactccttac tcatatgcaa agggccgggt gttacaaagg gaagttcgat aatcttgttc
     4201 acaaaatctt tggtgttttc caccttgctg ctgttgacaa tttccttgcc cagcgtggaa
     4261 atacattcca gcaccgtaga gctgtgttct ttttgcagtt cgtgaaataa cttgaaaatg
     4321 ttatctataa agggctttac ctgttccggc tcaatttttt ccagaatcct tttaatcagg
     4381 atgttcagat ttataagcag ggtgttggtt acatgagcca ttgcaggtat gtgcaacagg
     4441 taaaccaaaa aataaaagcg gttggcaatt atttgaaaag tatcggtacg tccgataaaa
     4501 tattttgtaa tctccgagta caacggaatg tttttgtcga gctcttcaaa agtttttacc
     4561 tgacgccatt tttccatcag gtcatcaaaa taaggcttgc cgataaatga taactcatca
     4621 gcaaaatctt cgctgaacag gtctttgttt ttttcgtacc aggtttcggc ccaggagttt
     4681 tcagcccaat acgaaataac acgtccggca agaaggtgaa tcacttcaaa aaacttgtct
     4741 ttcagtacgg gattgttgtt taataccgat agctggtttt ttataaaggt ggcatttcgc
     4801 agcaccgaaa cttcattatg ttccccccat ctttttatga tgtccaggcc cttggattcc
     4861 agttgcattt gaacggtttc cagcttggta atcatcgcca gaaattcaat catggccata
     4921 aaaaccattt caaaagcctt ggtttgaacc gttttttcca acgattcttc gaacaactgt
     4981 aaaatgatat ccagggcttt agcctgatcg ggacactggg tataaaacca aaagtcgcct
     5041 aatgccagcc gccgtaactc gtcactgaca tggtcccagt tgatgtatcc gtgatagtat
     5101 tcatcaaaaa aattcttagt tctttcgtgg atgccgtaat gtgattccga aaggcccaac
     5161 agccatacat gttcctctct caacacgatg tctttgggaa gttgagttat ttttaggttg
     5221 gcagctaatc cggttgattt gataggtctg ttcatgttca attaaaaaaa ttttttctca
     5281 caaagagtat aaaccggtac aaaataagaa aaaattcagg ggtaaattgt actttgtgcc
     5341 gacaaacaaa attagacttt tttttgatgg gaaaaaaaat tccgcgacac acaccgcgaa
     5401 acacgcatta agcttgcaaa agaacaaact tagcaggccg tactcagggc aaaaatattt
     5461 taataattcc ggtatggcag cgggaggata tttttgcaat gaataatccc ggcatatctt
     5521 ccggcagcgt gatttctaaa ggactttcat tgatatttgg aatctttttt ttaaaaatag
     5581 tacgccctgt cagatcaaac agatccacaa gattttctga accatcatgt tttccgcata
     5641 taattaaaga tctttttgcc ggatcaaaat aaaatatacc atcttctgaa atagtctccg
     5701 gtgtccctaa tgacagttca ttgaaacgcg tcataaactc ttcaaaatac attgcggcga
     5761 tttgcccgtc gtgaattatc agcgtgtttt cgtcgtaatc ttcttcggct gagttggtcc
     5821 agttgtgaga gcctgtaact accaaaggat ccgaagatgg actggtagca tccgcaaccc
     5881 catatttatg atgaaatgaa ttaggaacat tttcatgcga taatacagga ataccggccg
     5941 aaagaagcga attatattcc gaaccgaaat aggaaacatt ttcgataatt cccttaacgg
     6001 caacgcccga attatggaca tcttccaccg catcccctat cccgttatgt ataaacgtga
     6061 acatggcaaa acagaaatca gaatctgtag tattgacagc agtctcgata tgggtggacg
     6121 ttccgtcgct gggtgaaaaa tatagttcaa cggttttgcc acctatggta aacgtgtggg
     6181 gcgtattgtc tgttttggca tccccgaatt ttgagttgga caaattgggg gttgccgttg
     6241 ccgagcccca catttcctcg aactccgttg tataagctaa tgccagttgt ttatcctgga
     6301 taaaaacaac attattagga tcaatggtta agttatccgg cgtatgattt gccgagcctg
     6361 taatcaccca ggcattgttt tcttcatccc tgtcaatgat tacaaacttg ttgtgcatga
     6421 tgtttgtgcc gtcatttctt ttaagcaggg caacagacgg atttaatccg gccagcgaat
     6481 aattcagcgc tgtggtttcc gaaataaaac gcacagttac tcctctgata tatgcctggt
     6541 ttactgcgga gatgatggcg tcgctcctgt tgtcgtaaac gcacatgtcg agcgtgtgat
     6601 gtgctttatt gatataggca acaatagtat cttccatgtg gggattatac atgccgtcag
     6661 catacgggga tatggaattg tcaaccgtat tggtgaaata tacccggata atacctgatg
     6721 atttggatgt gaatgattgg gccgaagcct gcgttgtggc taccagtagc gcagtccaaa
     6781 cacacaaaaa ggagaagatt gtagttgctg tttttttatt gatgatctta aaaaaagcta
     6841 ctgacatcgg caatatcaac ccttcaggtt gattttaagt ttttgcccgg gctgaataag
     6901 gttttttgaa ttcaaattgt tcaggcggcg gatgtcttcc actgtaaccc cgttgtattt
     6961 ttcggctatc ccccacaagg tgtcgccttt ttgaaccgta tgataaatat atttttcaga
     7021 agcagtagtt ttgggagaag caggttttgc ctgtatagcg gcctgggctt gtgccggagc
     7081 cgttgtggtg gtctgttcag cagcctgcgt tgtggtgggt gcaggggctt gtgttttgct
     7141 tactgaggct ttggccatca ccgggctggg cttggttttg cttgccgaca tcagtttcag
     7201 ttgttgctga taagcagctt cctgtggtga tttataacaa tagatggcat cctcgttgct
     7261 gataaattcg cccataattt ttcgtggaag gcacagcaca taaggttttt cttcggtggc
     7321 aggtatgatg ccttttttat aggccggatt cagaaattta atttcttcag ccggcataca
     7381 taggtacgaa gatatctggc cgatattgta gtttttggtt accgtaaccg tatcaatttc
     7441 gcaatgcagt atcggaggtt gtgtgggata aagattatgt tcggcggcgt atgtcattac
     7501 gtaggacgct ccaataaatg ccggcacata gttttgtgtt tctacaggca aaaatctctg
     7561 aattttccag taggtaatac gttcgctgct gtcgagattg gcaatgcgta tagctttatt
     7621 tatgcatccc gccccggcat tgtatgcggc aagtactatt gcccagtcct ggtagatgtt
     7681 gaaaaggtct ttcagatgcc tgcaggcagc cactgttgat ttatagggat caaacctgtc
     7741 gtcaacatac gaagatacct ggagatcata cattttgccg gtggttagca taaactgcca
     7801 caatccgctg gcgccacagc gcgaacgggc aatagggttt aaggccgatt caatgatggc
     7861 cagatatttt aactcaagag ggaggtcgta attgtctagt tgttcttcaa aaagcggaaa
     7921 atatatttct gccagtccca gcatacggga agtgagtttt ctcttctgta ccgaatacac
     7981 atcaatatag tttcttacct gctggttgta aacatattca aaaggagagt tgatactcat
     8041 aatattgatt cgtttgctgt aaacactgtc gtcgtaaaca ggcaccgaat cggccggaaa
     8101 gttatagatg tttaatttgg aaacttcggt tgtaaaggta gaattagtaa aaaaagccag
     8161 ggtggcaagg ctgtcgaatt gggcaaccac cggattgtct ttataaaaag atgaatcggg
     8221 atgatgctgc gggagagaag aagaaaacgg aaaagataag gctgaaaaag caaaaaacaa
     8281 tgatgaaaat aatgaaagca aactcttttt gtaaaaaatc atgcgataaa atattgattt
     8341 agttgatatt acatgttaat ttaggaaaat tgatttcaca aattaacaaa taaaattcgt
     8401 aaaacgcaaa atttttttcg ttttttttga aaaaaagttt tataacaact taaaaatcaa
     8461 aatggtgtct tgtacaatgt tgataggtag ttgtcggcaa attcgcgaac acaggcctgg
     8521 ccgccgcatt tcgatagtat tattgtaact ttttctttta cagcctcggc ggcatctgca
     8581 ggacaagccg acaggcccac tttttctatc accgggatat cattaaggtc atcgccgata
     8641 taagcaacat tttgcatggt tattttcatc tcattacacc atttttcaag cacttccagc
     8701 tttttccagg tgccaatata cactttcttc actcccagaa tttttgcccg gtgggttatg
     8761 gtgttttgag taaatcccga actgagaaaa cctacctggt agcctgcatt tatcaggctg
     8821 atgatggcaa gaccgtcccg ggcatgaaat ttctttatct catcgccatt ttccgtatag
     8881 ataatgcccc catcggtcat cacgccatca atatcaacca caagaaattt aaaatcaaag
     8941 gtctggacca gccgctccct ggcttgaata ttaacggtga ggaattgggc aatgggataa
     9001 ttcagcgttt cagaaatttt tatcagctgt tcagcatcgg gcaatgcctt ttttttcaaa
     9061 aaatcacgga taagactttc tgaaatcccg gtttgttttg cccattgaac agcatctgtt
     9121 tttcggcggg ccgacaggta cttaaaattt tctgaaaaaa aacatttcat attatgttcc
     9181 ggttaatggc tttggcaatg ggtttaaggg tttcaaacaa tgcttcaaat tgctgatggg
     9241 tgagttgctg cgacgcatcc gaaagggcca ccgatgggtt ggggtgcact tcaatcagca
     9301 ggccgtcaac gcccatggcc atgcatgccc gcgcgaggtc gggaacgcca taggcataac
     9361 ccatggcatg gctgggatcg agtattaccg gcaggttggt gttttctttc aggtaagctg
     9421 ctccgcacaa atcaagggta aagcgcgttt tggtctcaaa ggtgcgtatg ccgcgttcgc
     9481 acaacatcac attggggttg cctcccgaaa ggatgaattc ggcggcctgg ataaattcct
     9541 gtaaggtggt gccaaaacct cttttcaaaa ggacgggttt ctgcgttttg ccgcattttc
     9601 gcaatattcc gtggtcgtac atcgacttgg cgccaatctg aatgatatcg gcatattcga
     9661 tcacgtcgtc cacatgcgac gaatcgcgca cctcggtaat aatggtgtac ccataaagcc
     9721 ggcgcatttt atccagcaat tccagacctt taacacccag cccctgaaaa gtatatggtg
     9781 aggttctggg cttgaaacag ccgccgcgca tgctcttcag tccgcatttt tttatgaatt
     9841 ctgccgattg ggttatctga ttttccgatt ccaccgaaca tggcccaatg gcaataaaca
     9901 cattgttggt attgccacct atgtagtttt caccaatcct gatagtcctt ttttccggaa
     9961 tatatttttt actggccaat tgtatgtcgg tgtcaaatac aaaagatgaa gtgaaaaaat
    10021 ccttgtcggc ctcttcggtt tttt
//
LOCUS       DAOSFP010000029        10034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5721_length_10034_cov_3.912316, whole genome shotgun sequence.
ACCESSION   DAOSFP010000029 DAOSFP010000000
VERSION     DAOSFP010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5721_length_10034_cov_3.912316"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PLI16_01645"
     CDS             <1..787
                     /locus_tag="PLI16_01645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOH83294.1"
                     /translation="PAWNYTYQDMNGSVARVSLGAKKASAKGILVCNSAGNSGNNDWH
                     YIGAPADAENILTIGAVDKNKKIADFSSRGPTADGRIKPSVCAMGEGTFVSSMAGSIM
                     SGNGTSFSSPVMAGAVACLWQANPEKSNMKIIEAVMASASKFLNPDADYGYGIPNLAA
                     ANIILKGNPIHNFDKDNALTVFPNPFDREFRIVFYSSDTSAVDVQLLDLAGKVVYENN
                     DIVRSPGCNDIHITLADELAKGYYMLKVASDSNTFTSKIMKIQ"
     gene            complement(836..1087)
                     /locus_tag="PLI16_01650"
     CDS             complement(836..1087)
                     /locus_tag="PLI16_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83295.1"
                     /translation="MKFLKKWYIILLAVIITEIAFESCNRHYRYKRMIKRRRTAISKR
                     YHSPYQKKLKKNIIPINKNYIIKNKRNTKGVPGYGNRRK"
     gene            1425..3659
                     /locus_tag="PLI16_01655"
     CDS             1425..3659
                     /locus_tag="PLI16_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH83296.1"
                     /translation="MKKNSLFLVFVVFFAVQISGVGVYGQLSDTLVIGPSPSDDYPTF
                     TAAVSALNAQGINGNVVFDVKPGVYNEQISINQIAGSGPAAAITFRAQDNDSTSVVLQ
                     YPSSASSGNNYVIRLNAADYCTFDKMTIKRTDTLKYCQVIELYGNARKNTFSNCIIEG
                     NANPVNTQLISTVVGSNNTGFKSYNNFLNNIIKNGSVGLSFTGQSNSSPDSCLLIRNN
                     KFINQSLKGIHLFAHRFAYIDGNEVVTNTVFNNYCGMFFQNANDTMRLLSNKISLSTG
                     DGMSFNNCNETAAANILIANNFISIGGNSNAHGIYILNSKNLYVLHNSINIYNTVTIY
                     GACIHINGSTTSNLNIKNNVLVNAGTGQGYVYYVSGTTGAPLAEVDYNALFINSGQWI
                     GYWKTSGITTLANWRTASSGDAHSFIANPGFVSNTDLHASNPLIDGAGTPSLNPFIIT
                     RDIDGDVRNAGAPDNGADELAGNDLGVTFIKPPAAGGYCVNQLFSPEVYVANYGTIAF
                     SGTIPLYYKIDALPLISDTMPNTLIAPGDSVLFTFSTPAMLTASGIHKLLAGTGVSSD
                     IVMTNDTSSRMVMAVTFPETSFTYSINDLEVSFTNTSVSGYTYLWNFGDGNTSIDEDP
                     VHTYANIGNYAVMLKTTNACGSDSITQNLALLGTAELPENSGISIYPNPVSDLLKVTL
                     ENGLPEMGTISIYSGTGMLLYKNDVTNNRQIVIDMADMPGSIYFVVLQIDKKLFYKKV
                     LKIN"
     gene            3818..4735
                     /locus_tag="PLI16_01660"
     CDS             3818..4735
                     /locus_tag="PLI16_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH83297.1"
                     /translation="MIKKLIRIIFFIVTGIGFSCTELFSQGNGESFQIIESVNDSIDL
                     RSNFYDDTDSLSDFFPASEIYESWDNNGIYYPHVDFSKKTDTTIIILSDNATNFYVHP
                     MKGRINSEYGYRRRRFHYGIDVDLNIGDSVKSAFDGMVRIAKYHRGYGNVVVVRHFNG
                     LETVYGHLSGFRVRENQMVKAGTLLGYGGSTGRSTGPHLHFEVRYLGTPMNPRKVIDF
                     EKFQLLNDTLYVTNRTFDKSRSQTYAHKSGTTTKTPYKGNGSYHYVKKGDTLGHIAMK
                     YGTSVSTLCKLNRLTSKSLLQIGQKIRIR"
     regulatory      4797..4993
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5096..7018
                     /locus_tag="PLI16_01665"
     CDS             5096..7018
                     /locus_tag="PLI16_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83298.1"
                     /translation="MKKSIIGIFILFAAAAAGQKSITDSVYTLGSVDVATDRFSNTLC
                     GVKVDKIDSVSMRVFSAGNLSQLLMQNSSVYIKSNGIAGLSSVSVRGTGTSHTAVLWN
                     GFNIQNPMNGGMDFSVIPVNFMNKISVQYNGMAALYGGGALGGAIHLSNEPVYGKGLD
                     VAIGASYGSFANYSVNFKLGISRKKGALVVRAFYQEGKNDFPFINNAQYGSPKVRQTN
                     SEMVSYGVMHDKAFRINERNQLTFRLWIQESRRQIPPLMSQEYSSVRQKDEFFRPALE
                     WENKGKKYKMYVRTALLVDEYKYEEPDKLINSASGTTASVSEYEIYFKLFPYHTMNAG
                     VHYKYTSGYCDYYTQKHSQQELSAFVSYTATSKTDKWKLNLAMREEYSNYSFVPLLPS
                     FAMDIRLYKDLLMYVNFSRNYRIPTLNDLYWYPGGNTDLKPENGYAEEFGIKQHVIWN
                     KTRLDYTVSGFNNNTQNLIIWLPESSYWSPKNIQAVWSRGAEINLKVNTELRSFILIF
                     SGKAAYVKSTVQKSNNKSSIGHQMVYTPEWIANTGFDMIYKTFNVNFNVEYNSRRYTA
                     YDNTDFLDPYFIGNLFASKDFYYKKFTFNVFFQVNNIWNQKYFSVAWQAMPGINFKTG
                     VTVNFKHSLTNKKIKQ"
     gene            7015..8016
                     /locus_tag="PLI16_01670"
     CDS             7015..8016
                     /locus_tag="PLI16_01670"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOH83299.1"
                     /translation="MKKLFLFFSGMVFTILFLSNNRVEAQTIADFENLPLPADTFWNG
                     SDLSGFFSSGNASFTNNFTDWGGGITSWNGFSYSNKRDTTTQSYTNEYSAITALGFDS
                     SDKYAVCYVSAFDPLPRIRLNGIAKGDTVSGFYITNSTYGYLTMKNGGGPAKKFGGVN
                     GSDPDWFALSVYAYKDGNKKNDSVLFYLADFRFPSPAQDYIVKDWQWVDLTSLGMVDS
                     LEFKLFSTDTAGGFGINNPTYFCMDNLITNHNSFAGVSENIPENITVYPNPAEDWIVV
                     NPGDTNSRIRIFDMKGNWIIPQSSGNSLVNLSGWAPGIYIVEVSNNNSVFYSKFIKK"
     gene            8019..9008
                     /locus_tag="PLI16_01675"
     CDS             8019..9008
                     /locus_tag="PLI16_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83300.1"
                     /translation="MRVLLVSIILFVFFPLFVKGQFPPPAGQPGTTAIHKDSSVFVGW
                     ASQCFVERGWQHIADTSLGRASVGDSSMAVGPAGTNGVVSLGDGGTAVVTFDSPIVNG
                     PSWDFAVFENSFSDTFLELAFVEVSSDGENYFRFPATSLTDTLVQVGAFDEVMDAAKI
                     NNLAGKYRGMFGTPFDLQELEGTPGLDVNYITHIRVVDVVGSIETGYATYDAFNHKIN
                     DPWPTPFPASGFDLDAVGVIHQLNSSIARVDDKTYISVYPVPANNYLYVVTEKCSAHI
                     AISLVSAEGKIMKTTRAESCNTCIDLSDVPAGLYLIRVSDESNNVVKKITIIK"
     gene            9005..>10034
                     /locus_tag="PLI16_01680"
     CDS             9005..>10034
                     /locus_tag="PLI16_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83301.1"
                     /translation="MKKFFLFFAGVFMMACMYSCKKDTPPDDTDIDISASHGVFIVNE
                     GNFQWSNASVTYYNFSDQEYQEDIFQIVNNRPLGDVAQSITVYQGKAYIVVNNSNKIE
                     VVNISNFASCGVISGFVSPRYFVPVSPTKAYVSDLYSNQISIVDLNTNQISGTITVNG
                     STEEMLYHEGTLFATNTRTDKLYFINTLTDAVSDSLQLGFASNSLVLDKNDKLWVMCA
                     GDANNSVNASLHRINIGQKTVEQTYPLGSPLDIWDKIAINATKDTVYYMYHGIYRLPV
                     SSAALPGAVFIPEGNSVFYGLAVNPYSNTIVVADAVDYVQKGKVNYYSASGALLGTIN
                     AGVLPSDFY"
BASE COUNT         2870 a         2017 c         2183 g         2964 t
ORIGIN      
        1 cccggcatgg aactatacct accaggatat gaatggcagc gtggcacgag tgtccctggg
       61 tgcaaaaaag gcttctgcca aaggcatcct cgtgtgcaac agcgccggca attcgggcaa
      121 taacgactgg cattatattg gcgctcccgc tgatgccgaa aatatcctga ccataggcgc
      181 agtagataaa aacaaaaaaa ttgcggattt cagttcgcgc gggcctactg ccgatggaag
      241 aataaaacct tcggtgtgcg ccatgggtga aggtactttt gtgtcgagta tggcaggcag
      301 cattatgtcg ggcaacggca catccttttc ttcgccggtc atggccggtg cagtggcttg
      361 tctgtggcag gctaatcccg aaaaaagcaa catgaaaatc atagaagctg ttatggcgtc
      421 ggctagcaaa tttcttaacc ctgatgccga ttatggctat ggtattccca atctggccgc
      481 tgccaatatt atcctaaagg gcaaccccat ccataatttt gataaagata acgccctgac
      541 ggtgtttcct aacccttttg acagggagtt ccggatagtg ttttattcca gcgatacctc
      601 ggcggtggat gtgcaactgc tggacctggc cggcaaggtc gtttatgaaa ataatgacat
      661 tgtccgaagt cccggatgca acgacattca tatcactctg gccgatgaac tggctaaagg
      721 ttattatatg ctcaaagtgg cttccgactc aaataccttt acgtccaaaa tcatgaaaat
      781 tcaatagaac atgaatcaaa aaaaaagagg ctcaaatgcg gcctcttttt tttatttatt
      841 ttcttcggtt gccataaccc ggcacccctt tggtgttgcg tttatttttg ataatataat
      901 tcttattgat gggaatgata ttcttcttca attttttctg ataaggagag tgataccttt
      961 tagaaatagc agtacgtctg cgtttgatca ttcttttgta acggtaatgt cggttgcatg
     1021 attcaaaggc aatctcagta ataataaccg ccaacaatat gatataccat tttttcaaaa
     1081 acttcataat actattttat cttttatgcc aatgcaaaaa ttctacaaat tcaaatatac
     1141 gtcagtaaaa ctaatacttt tttttagaaa agaacaaaag gatctaattt tttcgatttt
     1201 aataatgagt tttatactga ttctattctc ttaaggttac aaaagaaaaa cttttttcat
     1261 tataaaacgc atttttttga aaaaaaatat taacaactat agtattaact tataaatcaa
     1321 tgaaaaaagt ataactaatg atgaattttt gcagatgaac ccaaaaaaat attccattaa
     1381 tgaaaaaatt ttatattttt gaaaaataac tgaactctgt ggatatgaaa aaaaattctt
     1441 tatttttagt atttgtagtc ttttttgcag tccaaatttc tggcgttgga gtttatggac
     1501 aattgtcaga cacattggta atcggcccat cgccctccga tgattatcct acatttacag
     1561 cagctgtcag cgccctgaac gctcagggta tcaacggaaa tgtagttttc gatgtcaaac
     1621 cgggtgttta caacgagcag atttccataa accaaattgc aggatccggc ccggcagcag
     1681 cgatcacatt ccgggcgcag gataacgaca gtacctccgt agtgttgcag tacccgtcgt
     1741 cggcatcatc gggaaataat tacgtgatac ggctcaacgc agccgattat tgtacgttcg
     1801 ataaaatgac catcaaaaga acggatactt taaaatattg tcaggtcata gaactttatg
     1861 gcaacgccag aaagaacacc ttttccaatt gcatcatcga aggcaatgcc aatccggtca
     1921 atacccaact gataagcacg gtggtgggtt ccaataatac cggttttaaa agctataata
     1981 attttttgaa caatatcatt aaaaatggtt ctgtcgggct tagctttacc ggccagagta
     2041 atagcagccc agattcctgt cttcttatcc ggaataataa atttattaat cagagtctta
     2101 agggcattca tttattcgca catcgctttg cctatatcga tggcaatgag gttgtcacca
     2161 atacggtgtt taataattat tgcggtatgt tttttcaaaa tgccaacgac accatgcgcc
     2221 tgctcagtaa caaaatttcc ttgtcaacag gcgatggcat gagttttaat aattgcaatg
     2281 aaactgcagc cgccaacatt ctgatagcaa ataattttat atctatcggc ggaaactcca
     2341 acgcccacgg tatttacata ttgaacagca agaatcttta tgtgctgcac aacagcatca
     2401 atatttataa tacggttact atttacggtg cctgtatcca catcaacggc agcactacct
     2461 ccaaccttaa tataaaaaac aatgtattgg taaatgcggg aaccggacag ggttatgtgt
     2521 attatgtttc gggaaccacc ggcgccccgc ttgccgaagt ggactataac gccttgttta
     2581 taaacagcgg ccagtggata ggctactgga aaacttcggg aatcactacg ctggcaaact
     2641 ggcgcaccgc ctcttcagga gatgcccatt cttttattgc caaccccggc tttgtttcca
     2701 ataccgattt gcacgcttcc aaccctctga ttgacggtgc cggcactcct tctctgaatc
     2761 cttttattat tacacgcgat atcgacggcg acgtgcgcaa cgccggagcc cctgataacg
     2821 gcgccgacga gttggctggc aacgatttgg gtgtcacctt tatcaaaccc ccggcagcag
     2881 gcggctattg tgttaatcag cttttttctc ctgaggttta tgttgccaat tacggaacta
     2941 ttgcgtttag cgggactatc cctctttatt ataagataga tgctttgcca ttaatcagcg
     3001 atactatgcc taatacactt atcgcccccg gcgactcggt tctgtttact ttttcaactc
     3061 ctgctatgct taccgctagc ggcattcata aattgctggc cggcactggt gtgagttctg
     3121 atattgttat gaccaatgac accagcagcc gtatggttat ggccgtcaca tttccggaga
     3181 cctcttttac ctatagcatc aacgacctgg aggtgagttt taccaatacc tcggtttcgg
     3241 gctataccta tctctggaat tttggtgatg gcaacacaag tattgatgaa gatcctgtgc
     3301 atacctatgc aaacataggc aactatgctg ttatgctaaa aacaacaaat gcctgcggct
     3361 ccgacagcat cactcaaaac ctggcgcttc tcggcactgc cgaactgccc gaaaatagcg
     3421 gtatcagcat ttatccgaat ccggtcagtg atttgctgaa ggttacgctg gaaaacggtt
     3481 tgccagaaat gggtaccatc agtatctatt caggcaccgg aatgttattg tataaaaacg
     3541 atgtaaccaa caaccggcag attgtcattg atatggctga tatgcccggg tcaatatatt
     3601 ttgttgtttt gcaaattgac aaaaaattgt tttataaaaa agtgctgaaa ataaattaga
     3661 tgcggtttgt gccaaacgtt ttgcggatag gtttcttaaa aaaaacttgg tcagtttgca
     3721 gaaaatcgta tttttgtaaa tgaaaaatta atcatccaca acctgtaact attgagttta
     3781 ctgtgttata agtgttttga ggggggttgg aaccaaaatg attaaaaaac taatacgaat
     3841 aattttcttt attgtcacag gaataggttt ttcctgtacc gagctttttt ctcagggtaa
     3901 tggagaatcc tttcagatta ttgaaagcgt aaacgacagt attgacctgc gttcaaattt
     3961 ttatgacgat accgactcct tgtcggattt ttttccggcc agcgaaattt atgaatcgtg
     4021 ggacaacaat gggatatatt atcctcatgt tgatttttca aaaaaaacag atacaactat
     4081 tattattctg tcggataatg ccaccaattt ctatgttcat cccatgaagg gcaggattaa
     4141 ttcggagtat ggctatcgtc gccggcgctt tcattacggc atcgatgtgg atttgaatat
     4201 tggcgacagc gttaaatcgg ctttcgacgg gatggtaagg atagccaaat accatcgtgg
     4261 ttacggaaat gtggtggtgg tacggcattt caacggtctg gaaaccgtat atggacattt
     4321 atcgggattc agggtgaggg aaaatcagat ggtaaaggcc ggaaccctgc tgggctatgg
     4381 agggagtaca ggccgctcca cgggcccaca cctgcatttt gaggtgaggt atctgggaac
     4441 acctatgaat cctcgaaaag tgatagattt tgaaaagttc cagttgctaa atgatacttt
     4501 gtatgttact aaccggactt ttgataaatc gcgcagtcag acttatgcac acaagtcagg
     4561 tactaccaca aaaactcctt ataaaggtaa cggaagttat cattatgtca aaaaaggcga
     4621 tacgctcggc catatagcta tgaaatatgg aacttcggtt tccaccttat gcaagttaaa
     4681 ccgcctgacg tccaaaagct tactacagat aggacaaaag atacgtatcc gctgatttat
     4741 caacaatttt ttttgaaaac ttttttttca caaagaactt cattagtaaa aatgcgtact
     4801 tttgtgattg aaatagtgcc cataaaaatg tttttatggt aaagggaatc aggtgagaat
     4861 cctggacagt acccgctgct gtaattccta cgtaaacttg aaggcattcc tggtcactgt
     4921 cccgccttgg cgggatggga agacgcctga aaggggataa gtcagaaaac ctgctgtttc
     4981 actattatat tgtttaaagc tttcggggaa aaaagcttaa tcagataggt tgtttcttgt
     5041 tatctgtttt tttcctgctg ctttaggcaa tacaaagagt taagttattt gtattgtgaa
     5101 aaaaagtatt ataggcatat tcattctttt tgctgctgct gcagcgggcc aaaaatccat
     5161 aacggattct gtgtacacgc tgggcagcgt ggatgttgcc accgaccgtt tttccaatac
     5221 tttgtgcgga gtaaaggttg ataagattga ttccgtttcc atgagggttt tttcagcggg
     5281 caatctgagc cagcttctta tgcaaaactc atcagtctat atcaaatcca atggtatcgc
     5341 aggattgagt tccgtttcgg taaggggtac gggcacttca catacggcgg tactttggaa
     5401 cggcttcaat attcagaatc ctatgaacgg aggaatggat ttttcggtga tccccgtgaa
     5461 ttttatgaat aaaatttctg ttcagtacaa tggcatggcc gctttatacg gaggaggcgc
     5521 attaggaggc gccattcatc tttccaatga accggtgtac ggcaaagggc tggatgtggc
     5581 tatcggtgct tcttatggga gttttgcgaa ctattcggta aattttaaat taggaatcag
     5641 caggaaaaaa ggcgcattag tagtcagagc attttaccag gaaggaaaaa atgatttccc
     5701 attcattaat aatgcacaat acggttcccc aaaagtccgg caaacaaatt ctgaaatggt
     5761 cagttatggc gtcatgcacg ataaggcatt tagaataaat gaaaggaatc aactgacttt
     5821 ccgcctgtgg atacaggaaa gcagaaggca gattccgcct ttgatgtccc aggaatatag
     5881 cagtgtcagg caaaaagatg aattttttcg tccggccctg gaatgggaaa acaaagggaa
     5941 gaaatataaa atgtatgttc gtaccgcttt gcttgtcgat gaatataagt atgaggagcc
     6001 cgacaaactc ataaattcag ccagcggtac cacggcatca gtttcggaat atgagatata
     6061 ttttaaactg ttcccgtatc atacgatgaa tgccggtgtg cattataaat atacctccgg
     6121 atactgcgat tattatactc aaaaacattc tcagcaggaa ttgtcggctt ttgtttccta
     6181 cactgcaaca agtaaaaccg ataagtggaa gttgaacctt gctatgaggg aagaatacag
     6241 taattatagt tttgtgcctt tattgccttc atttgctatg gatatccgtt tgtacaaaga
     6301 tttactgatg tatgtaaact tcagcagaaa ttaccgtatt cctacattaa atgacctgta
     6361 ctggtatccc ggcggtaata cagaccttaa acccgaaaac ggatatgctg aagaatttgg
     6421 gataaaacaa catgtgatct ggaataagac ccggttggat tatacggtat ccggatttaa
     6481 caataatacg caaaacctga tcatctggtt gcccgaatca tcgtactggt cccctaagaa
     6541 tattcaggct gtgtggagcc ggggcgcgga aatcaatcta aaggttaata cagaactaag
     6601 aagttttatc cttatctttt caggcaaagc agcctatgtt aaatccactg ttcaaaaaag
     6661 taataacaaa agcagcatcg gccatcagat ggtatatacc cctgaatgga tagctaatac
     6721 tggctttgac atgatctata aaacatttaa tgttaacttt aatgtagagt acaattccag
     6781 acgatatacg gcttacgata atacggattt tctggatcct tattttatcg ggaatttgtt
     6841 cgcttcaaaa gatttttatt ataagaagtt cacttttaat gtttttttcc aggtcaataa
     6901 tatctggaat cagaaatatt tttcggtggc atggcaggct atgcccggaa ttaattttaa
     6961 aacaggagtt acagtaaatt tcaaacattc attaacaaat aaaaaaatca aacaatgaaa
     7021 aaactatttc tttttttcag cgggatggtc tttacgatct tgtttttatc aaacaaccgc
     7081 gtagaggcgc aaacaatagc cgattttgaa aaccttccgc tgccggccga tactttctgg
     7141 aatggatctg atttaagcgg ttttttctca agcggaaatg cttcatttac gaataatttt
     7201 actgattggg gaggtggtat tacatcgtgg aacggatttt cgtattcaaa taaacgcgat
     7261 accacgacac agagctatac caatgaatat agtgcgatta ctgctttagg atttgactcc
     7321 tcggataagt atgccgtgtg ttatgtgagc gccttcgatc ctttgcctcg tatcagattg
     7381 aatggtatag ccaaaggcga tacagtcagc gggttttaca tcactaattc gacctatggc
     7441 tatctgacga tgaaaaatgg cggcggtccg gcaaaaaaat tcggaggtgt aaacggctct
     7501 gacccggatt ggtttgcact gtctgtatac gcctataaag acggaaataa aaaaaacgat
     7561 agtgtcttgt tttaccttgc tgatttcagg tttccgtccc cggcacagga ttatatcgtc
     7621 aaagactggc aatgggttga tttaacttct ttaggaatgg ttgacagcct ggagtttaaa
     7681 ttattctcaa ctgataccgc cggtgggttt gggatcaaca acccgaccta tttctgcatg
     7741 gataacctga ttacgaatca caacagtttt gctggtgtca gtgaaaatat accggaaaac
     7801 ataactgttt atcctaaccc cgctgaggat tggattgttg ttaaccccgg ggatacaaat
     7861 tcgcgtattc gtatttttga tatgaaagga aattggatta taccgcaaag ttccggtaat
     7921 tcattggtga atcttagtgg ttgggctccg gggatatata ttgttgaagt gagtaacaat
     7981 aattccgtgt tttattccaa atttatcaaa aaataaaaat gagagtctta ttggttagta
     8041 taattttatt tgtttttttt ccactgttcg tgaaaggaca gtttcctcca cctgccggcc
     8101 agccaggaac cacggcaatt cataaagaca gttcggtttt tgtcggttgg gcatcacagt
     8161 gttttgtcga acgcggctgg caacacattg ccgacacgtc gctgggccgg gccagcgtgg
     8221 gcgatagctc catggctgtg gggcctgccg ggacaaacgg cgtggtgagc ctgggcgacg
     8281 gcggtaccgc tgtagttact tttgacagcc ccattgttaa tggtccgtcg tgggactttg
     8341 ctgtatttga aaactcgttt tccgatactt ttcttgaact ggcttttgtt gaagtaagct
     8401 ccgacgggga aaattatttc cgttttccgg ctacttcgct taccgatact ttggtgcagg
     8461 tgggcgcttt tgatgaggtg atggatgccg ccaaaattaa caacctggca ggaaaatacc
     8521 gcggcatgtt cggaacacct ttcgacttgc aggagttgga aggcacgccc ggccttgatg
     8581 ttaactatat cacccacatc agagttgttg atgtggttgg ctccatcgaa acaggttacg
     8641 ctacctatga tgcttttaac cacaaaatca acgatccgtg gcccacgcct ttcccggcct
     8701 cgggttttga ccttgatgcc gtaggggtga tacatcagct gaattccagc attgcaaggg
     8761 ttgatgataa aacctatatc agcgtgtatc cggttccggc aaataactac ctgtatgttg
     8821 ttactgaaaa atgttctgca cacattgcaa tatctttggt aagtgctgaa ggaaaaatca
     8881 tgaaaactac ccgtgcggag tcctgtaaca catgcattga cttgtcggat gttccggcag
     8941 ggttgtacct catacgtgtt tcagatgaaa gcaataatgt tgtgaaaaaa ataacgataa
     9001 taaaatgaaa aagttttttc tgttttttgc cggtgtcttt atgatggctt gcatgtattc
     9061 ctgtaaaaag gataccccgc ccgatgatac cgatattgat attagtgcca gtcatggtgt
     9121 ttttattgtt aacgaaggca acttccaatg gagtaatgct tctgttactt attacaattt
     9181 ttctgatcag gaataccagg aagacatttt tcagatagtt aataacaggc cgttgggcga
     9241 tgtggctcaa tccattacgg tttatcaggg aaaagcttat atcgtcgtga ataattccaa
     9301 caagattgag gttgtcaata tcagcaactt tgcgtcgtgt ggtgtaatca gcggatttgt
     9361 ttctccgcgt tattttgttc ctgtcagccc taccaaagct tatgtgagcg atttgtacag
     9421 caaccaaata agcattgtcg atttgaatac caatcagatt agtggcacca ttacggtgaa
     9481 cggtagcacc gaagagatgc tgtaccatga gggtacattg tttgctacca acacccgcac
     9541 cgataagctt tattttatca ataccctcac agatgccgtt agcgatagcc ttcagcttgg
     9601 ttttgcttca aactcgctgg tgcttgataa aaacgataaa ttgtgggtga tgtgtgccgg
     9661 tgatgccaat aacagcgtca acgcttccct tcaccgcatc aatattggtc agaagacagt
     9721 tgaacaaact tatccgctgg gctccccact ggacatttgg gataaaatag cgataaatgc
     9781 aacaaaggac accgtttatt atatgtatca tgggatttac cggttgccgg tgtcttcggc
     9841 tgcgttaccc ggtgcagtgt ttattccgga gggaaacagt gttttttacg gactggcggt
     9901 gaatccatac agcaatacca ttgtggtggc cgatgccgta gattatgtcc agaaaggaaa
     9961 ggtaaattac tattctgcct caggcgcgct actgggaact atcaacgcag gggtgttgcc
    10021 ctctgatttt tact
//
LOCUS       DAOSFP010000030         9890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5834_length_9890_cov_3.544281, whole genome shotgun sequence.
ACCESSION   DAOSFP010000030 DAOSFP010000000
VERSION     DAOSFP010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5834_length_9890_cov_3.544281"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLI16_01685"
     CDS             <1..998
                     /locus_tag="PLI16_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOH83302.1"
                     /translation="DEKDCLYIYAQTMNGKTQYGIVGCAGVTDYMNGIIKKHELTRKE
                     KEEDRMKHVRNTNANMEPVFFTYPAVKEIDDIVENIVKNQPAEYDFTSVDGFGHHFWV
                     IRDDETINKLVGLFGKIPYTYVADGHHRTAAAALVGNEKKQNNPNHTGTEEYNFFLAV
                     HFPANQLTIIDYNRVIKDLNGLTPEEFLKKLEKVFTVVLKGSEIYKPEKLHNFSLYLD
                     GKWYSLTAKPGTFNDADPIGVLDVTILSKLVLDEIIGIKDLRTDKRIDFVGGIRGLGE
                     LKKRVDSGEMKAAFALYPVSMEQLINIADTGNIMPPKTTWFEPKLRSGLVVHSLE"
     gene            complement(1046..1474)
                     /locus_tag="PLI16_01690"
     CDS             complement(1046..1474)
                     /locus_tag="PLI16_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOH83303.1"
                     /translation="MKNKLFISSATNTGLLILRIGIGIMFILHGIPKLAGGPATWDFL
                     GSQMDIIGVGLGATFWGFMAALSESLGGLLLILGYFTRPAAFLMLFTMFMATLMHLTN
                     GDGLKGASHAIELGIVFLAVILTGAGRFSLDHLIFAQRNK"
     gene            1612..3597
                     /locus_tag="PLI16_01695"
     CDS             1612..3597
                     /locus_tag="PLI16_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOH83304.1"
                     /translation="METQKEIKNKLKLILPFLVLLIFLQNGLYSQYQQVYDLRTVYFM
                     TPVKDQGNCGSCWAFATIASIESNWKKTGYGTYILSEDNVIDCHSFDEAPCYGGSFFM
                     AQALFSRHGGTLLNSSDAYTPTIQDCPLSQVFHPASSSYNEEIRFLPKNTNIIKQALI
                     DYGALATSMFFNIANYNSAKYKYYDNQLDATDSLYPHCVTLAGWNDTMTFQGAPGTGG
                     WIIKDSYGTAWAQAGYFYVSYYDAGILSETAFFPNRQDVPMSVNKSNVYFHDTYGWVD
                     NFGFGSNTAYGLVKYTISPSGSTISAQQIKRIGTYAVEDNTTITISIYKSFSAGVLSG
                     LITSQSLTCPFKGFYNLSFSLPSDSIGTDVYIKTQYQTPAGKNSPVPIEKYEANHTSG
                     IQLSSNSCWVSTNGTTWVPTGQGSGYPFDLCIKMYTENAPKATFNVNTDSICVGGSII
                     FNDVSAMPKDSVRWLLNGTYQGNMPVENIIFYTAGQHLIELVVFSGSNNDTTGITITV
                     LPLPSTPLITQLNDSLFSSYAYAYQWYDNSGIITVATNNYYVPLQSGIYYVLITDQYG
                     CSNMSSPYNFIINYIGNATTEVIELYPNPFGDYLNINCQSMPATVFISDVYGQIVLQA
                     TLSSYKSALYLPHLNPGIYYLQINTTGGRKVLKTLKL"
     gene            complement(3648..3779)
                     /locus_tag="PLI16_01700"
     CDS             complement(3648..3779)
                     /locus_tag="PLI16_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83305.1"
                     /translation="MLTKPCAVNHLDTADVHVPGWQVDEVFRTVHVHLPSTCHTGSL"
     gene            complement(3957..4159)
                     /gene="rhuM"
                     /locus_tag="PLI16_01705"
                     /pseudo
     CDS             complement(3957..4159)
                     /gene="rhuM"
                     /locus_tag="PLI16_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004345244.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
     gene            complement(4237..5157)
                     /locus_tag="PLI16_01710"
     CDS             complement(4237..5157)
                     /locus_tag="PLI16_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83306.1"
                     /translation="MASVTPYFFEEGQEYEFTVISRQSMPPGDDTYFVMDSPFQTRHL
                     LAEKHYIHYKIIPGQKIKCSIDKINCSGKIYLEPRHPAYEVGQEYDFPVKGVREIVNS
                     AGAGELMLVLEDCFKNEVYVNISGRVFAASETVRCRVDRIKKGKLFLTPVDFSRNIPV
                     YETGKYYQFILEGIVTLAEGDEYYVLRDEQGNAHHLRKKYFEHYGFHAGCIVYAQFLS
                     QPAPFRHYLEPLHPEYKTGGTYEFSFAGIESYINERGTETRKPFVRDGSGKEYAVQCA
                     DLEKFDIAVGSRIWCRVSSIRMARLALECL"
     gene            complement(5170..5646)
                     /locus_tag="PLI16_01715"
     CDS             complement(5170..5646)
                     /locus_tag="PLI16_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOH83307.1"
                     /translation="MHLVNRVEVQEQQAPREKTTIKKTAKTKVARVKTAAKKTTGAAG
                     VKKTKTADKASAAKTVVTPATKTKSSGTTKVTLKDVLTMKKTDLTKLVKDIDLKTLYC
                     ALVGADNKVKKSIITHLGKLQLKSYNKLFAENLPVSDSDVEKAQKVLLARIKQLLA"
     gene            complement(5653..6825)
                     /locus_tag="PLI16_01720"
                     /pseudo
     CDS             complement(5653..6825)
                     /locus_tag="PLI16_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598764.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
     gene            complement(6837..8114)
                     /locus_tag="PLI16_01725"
     CDS             complement(6837..8114)
                     /locus_tag="PLI16_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH83308.1"
                     /translation="MFGWEFPPHITGGLGTACFGLTKGLLKNGVEVLFVVPKSYGDED
                     QQAVRLINASDVRIDFTNKMYQEYWKQLTYIEIGSNLIPYVGEEEFQKMTAVNTIDKT
                     EEHSSVFSQQYTFSGKYGKNLMEEVTRYALVASTLAGSQQFDVIHAHDWLTYPAGIAA
                     KKISGKPLVVHMHATEFDRSGDHINQNVYDIERKGMEIADAVITVSNFTRDIVINKYG
                     INPDKVFTVHNAVEHNTGDSDLVIERNVKEKVVTFLGRITFQKGPDYFVEAAKRVLDK
                     DKNVRFVMAGSGDMLNRMILRVAQLGISTHFHFTGFLQGAEVDKMFLLSDVYVMPSVS
                     EPFGISPLEAMRSNVPVVISKQSGVSEVLKYALKIDFWDIDAMADAIYGLLHYDALSV
                     MFRKYGKEEVENLKWESASSKIRDVYNKVIFKN"
     gene            complement(8190..>9890)
                     /locus_tag="PLI16_01730"
     CDS             complement(8190..>9890)
                     /locus_tag="PLI16_01730"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOH83309.1"
                     /translation="PDGVLNPKGHKYISGLEMEPMPKFTYRVGGVIFTKEIMFCIESD
                     RLLIKYTLEDAHSPTVIRLQPFLAFRNIHALTKANPNADTHAEPVENGIKIRMYPGYS
                     TLFLQCSKKIQYTHVPHWNYNIEYVEEKERGYDYQEDVFVPGYFELPMSKGESVVFTA
                     GLNEIDGSKLKQIYSSETKKRIPRDNYKNCLINSGLQFFVTRNNKVEIIAGYPWFGRW
                     GRDTFIALPGLTLAINDPEKCKAIIDNMIAELKGGLLPNVGCGQNSAYNSVDAPLWFF
                     WALQQYAIYTGTQSMIWKEYGEKMKLILNAYRNGCEYNIKMQDNGLIYAGIEGKALTW
                     MDAIVDGKPVTPRIGLAVEINALWYNAVKFAIEVAKLAGDQDFAKEWDEIACKIPAAF
                     KGTFWNKDKGYLCDYVNGDYKDWSVRPNQLLAASLPYSPVSEQIRELILEITERELLT
                     RRGIRTLSPKSPDYKGIYKGGIHERDAAYHQGIAWPWLLGHYAEAYLKIHPNYGLPHI
                     KQLYESFEPVMMEHGIGTISELYDGDPPYLPGGAPSQAWSVAELLRIDYLIEKYSSVK
                     "
BASE COUNT         2806 a         2197 c         2049 g         2838 t
ORIGIN      
        1 aggacgaaaa agactgcctg tatatttatg cacagaccat gaacggcaag acacaatacg
       61 gcattgtagg ttgtgccgga gttaccgact atatgaacgg tatcatcaaa aaacatgaac
      121 ttacccggaa agaaaaagaa gaagaccgca tgaaacatgt tcgcaacacc aatgccaaca
      181 tggagcccgt tttctttacc taccctgccg ttaaagaaat tgacgacatc gtggaaaaca
      241 tagtgaaaaa ccaacctgca gaatatgact ttacctctgt ggacgggttc ggacatcact
      301 tttgggtaat acgtgatgat gaaaccatca acaagttagt tggacttttc ggaaaaattc
      361 cgtacaccta tgtagccgac ggacatcacc gcaccgccgc cgctgccctg gttggcaatg
      421 aaaaaaaaca aaacaacccc aaccataccg gcacggaaga atacaatttc ttcctggctg
      481 tgcactttcc ggcaaaccag ttgaccatca tcgactacaa ccgggtgatc aaagacctga
      541 acgggctgac accggaagaa tttctgaaaa aactcgaaaa agtttttacc gttgtactaa
      601 aaggaagtga aatatacaaa ccagaaaaac ttcataactt tagtctttac cttgatggca
      661 aatggtattc actcacagca aaacccggca cattcaacga tgctgatccc atcggagtgc
      721 ttgatgttac catcctttcg aagctcgtcc ttgatgagat tattggcatc aaagatttga
      781 gaaccgacaa gcgtattgac tttgtgggcg gcatccgtgg cttaggcgaa ctgaaaaaac
      841 gcgtggattc gggcgagatg aaagctgctt ttgccctgta tcctgtttcg atggaacagt
      901 tgataaacat tgccgacacc ggcaacatca tgcctccgaa aaccacctgg tttgaaccca
      961 aactgcgcag cgggcttgta gtacattccc ttgaataata aattattctg attataaaaa
     1021 aacgccggtc attgatccgg cgtttttatt tatttctttg ggcaaatatc agatgatcca
     1081 gactgaaccg gccggcgccc gtaagtatga ctgccagaaa cacaatgcct agttcgatgg
     1141 cgtgtgaagc gccttttaat ccgtcgccat tggtgagatg cattagcgtt gccatgaaca
     1201 tggtaaataa cattaaaaag gcagcggggc gtgtaaaata gccaagaata agtaataatc
     1261 ctcccagtga ttcggaaagt gcggccataa atccccagaa ggtggccccc aaccccactc
     1321 ctattatgtc catctgtgag ccgagaaaat cccatgtggc agggccgcct gccaatttgg
     1381 gtatgccatg gagtataaac atgatgccga tgccgattcg cagtatcaac aagcctgtat
     1441 ttgttgcgga tgatataaat agtttgtttt tcatgatttt tattatttag aattattcta
     1501 aacaaaaatc aggccattat ttagtctttt aaattatttg tgtgaaattt tagtaaatgc
     1561 ttggatgtca aacgaaatta tattaaattt ataatttaaa tgattaagaa aatggaaaca
     1621 caaaaagaga ttaaaaacaa gttaaaactg atattacctt ttcttgtact tttaattttt
     1681 ttacaaaacg ggttatattc acaatatcaa caggtttatg atttacgaac cgtttacttt
     1741 atgacgcccg tcaaagacca gggaaattgc ggctcatgtt gggcttttgc aacaatagcc
     1801 tccattgaat cgaactggaa aaaaaccggc tatggtactt acattttatc agaagataat
     1861 gtgattgatt gtcattcttt tgatgaagcg ccttgttatg gaggaagttt ttttatggct
     1921 caagcccttt tttcaaggca cggcggaaca ttattgaaca gcagtgatgc ctacaccccc
     1981 actattcagg attgtccgct atctcaggta ttccatcccg cttcgtcatc ttataatgaa
     2041 gaaatacggt ttttaccaaa gaacaccaac attattaaac aagcattgat agattatggc
     2101 gctcttgcca cttccatgtt tttcaatatc gcaaattata attcagcaaa gtacaagtat
     2161 tatgataatc agttggacgc aacagattcg ctttaccctc attgtgttac actggcggga
     2221 tggaacgaca ctatgacatt tcagggcgca ccaggtactg gtggatggat aataaaagat
     2281 tcatacggaa cagcctgggc acaggcggga tacttttatg tttcctacta tgatgccggc
     2341 atattgtctg aaacggcatt ttttccaaac agacaagatg tcccaatgtc agtaaacaaa
     2401 tccaatgtat atttccatga cacttatggg tgggttgata atttcggttt tggatcaaat
     2461 acagcctatg gtttggtcaa atataccatt tcaccttcag ggagtaccat ttcagcccag
     2521 cagatcaaac ggataggaac ttatgctgtg gaagacaata cgacaattac aatcagcatt
     2581 tataaaagtt tttctgcagg tgtattatcc ggcctgatta caagccagtc actgacctgc
     2641 ccttttaaag gcttttataa tctttctttc tctttgccat ccgactcaat cggaaccgat
     2701 gtttatatta agacacaata tcaaacgcct gcaggaaaaa acagccccgt accgatagag
     2761 aaatatgaag ccaatcacac atcaggaatt cagctttcat caaactcgtg ctgggtcagc
     2821 accaacggaa caacttgggt tccaacaggg caaggaagcg gatatccttt tgatctttgt
     2881 ataaagatgt acaccgaaaa tgctccaaag gcaacgttca acgtgaatac tgacagcatt
     2941 tgcgttggcg gaagcattat attcaatgac gtatctgcca tgccaaaaga cagcgtcaga
     3001 tggctgctaa atggcacata ccagggaaac atgcccgtag aaaatattat tttctataca
     3061 gccgggcagc accttataga actcgttgtt ttttcgggca gtaataatga cactacaggc
     3121 ataacaatta cagtattacc cttgccatcc acacccttga taacacagtt gaatgacagc
     3181 ttattttcat catacgcata tgcctatcag tggtacgata actcaggaat tattacagtg
     3241 gcaacaaata attactatgt gcctctgcag agcggaattt attatgtttt gatcacagac
     3301 caatacggat gcagcaatat gtccagtcct tataatttta ttatcaacta tatcggtaat
     3361 gctacaacag aagtcataga actataccca aatccctttg gggactacct gaatattaac
     3421 tgtcaatcaa tgcctgcaac agtctttatt tctgatgttt atgggcagat agtccttcag
     3481 gccactttat catcatataa atcggcacta tacttaccac atttaaaccc gggtatttat
     3541 tatttgcaaa taaatacaac cggaggcaga aaagttttaa aaacattgaa gttgtaaatc
     3601 gacgtcttcc ctgtaggtgc ttgttatatt aaacttcttg aaccaaacta taacgagccg
     3661 gtgtgacaag ttgacggcaa atgtacatgt actgtccgaa aaacctcgtc cacctgccag
     3721 cccggcacat gaacatcggc agtgtcgaga tgattgacgg cacaaggttt tgtcagcaaa
     3781 atttaatctt taataaattt tgataccaat tttttatcag catagtaaag cttaataaaa
     3841 taaactctgc tattaagcga tgatattgtt agttctgttt tcgattcggt taatggttgc
     3901 tttataaaca actgaccttg taaattatac aaagcaattg atgcgttttt tgacaaatcc
     3961 tgtagcaatt gttcaacgct cattgtcaaa attttatcca aatgttctgc cagtctttca
     4021 ttgtcatggt ttgttcttgc tcagcctgac gttccgtaaa atccaaatac cccgaaacta
     4081 tttgtccgag cgaacgaaac tctctttcgt ctagataatt ttttgctgcc actgcatctt
     4141 tgaggcacgg acgactgccg ggggaaagtc atcaaaaaat atcctatacg tttgggagtt
     4201 gaaccgaaac attcacattt catgagatta tatgtactac aaacattcca aagccaggcg
     4261 ggccatgcgg atactgctta cacggcacca tatcctgctg ccaacggcaa tatcgaattt
     4321 ttccaggtca gcacactgca cggcgtattc ttttccgctg ccatcgcgca caaagggctt
     4381 gcgtgtttcg gttccgcgct cgttgatata cgactcgata ccggcaaaag aaaactcata
     4441 ggtaccccct gttttgtatt cgggatgaag cggttcgagg taatgcctga atggtgccgg
     4501 ctgcgacaga aactgcgcat aaacaatgca tcccgcatga aagccataat gctcaaaata
     4561 cttttttcgc agatggtggg cattgccctg ttcatccctg agcacataat attcatcgcc
     4621 ttcagcaagg gtaacaatcc cttcaaggat aaactgatag tatttccctg tttcatacac
     4681 aggaatatta cggctgaaat caacaggtgt gagaaaaagt tttccttttt taatgcggtc
     4741 aacacggcat cttaccgttt cggaagcagc aaaaacacgg cctgagatat taacataaac
     4801 ttcatttttg aagcagtctt ccagcacgag catcaactct cctgcaccgg cggaattaac
     4861 gatttccctc acccctttca ccggaaaatc atactcctgg ccaacttcat aggcaggatg
     4921 ccggggttcg agataaattt tgccggaaca atttattttg tcgatactgc attttatctt
     4981 ttgtcccgga ataatcttat aatgtatata gtgcttttct gccagcaagt gtctggtctg
     5041 aaagggactg tccattacaa aatacgtatc atcaccgggc ggcatactct ggcggctgat
     5101 caccgtgaat tcatactcct gcccttcttc aaaaaagtat ggggtaacgg aggccattaa
     5161 caaaaatact tatgctaaaa gctgttttat cctcgccagt aaaacttttt gtgctttttc
     5221 aacatcggag tcagacacgg gaagattttc ggcaaacagc ttgttataac ttttcaattg
     5281 taactttcca aggtgggtga tgatactttt tttcacttta ttatcggcac cgaccagggc
     5341 gcaatacaag gttttcaggt caatatcttt aaccagcttc gtcagatctg tttttttcat
     5401 cgtcaaaaca tctttaaggg taactttggt tgttccggac gacttggttt tggtggcagg
     5461 agtcacaact gttttggctg ccgaagcttt gtcggctgtt tttgtttttt taacgccggc
     5521 ggctccggtc gtttttttag cggctgtttt tacacgggca acttttgttt ttgcagtttt
     5581 tttaatagta gttttttccc gaggtgcttg ctgttcctga acttcaacac ggttaaccag
     5641 gtgcatatcc tttagtctga tgataaaatc gctgaggata ttcatgaagt taatataggc
     5701 ttcataaggt gagttaaaag gattaaaata tttgtgaaca tcgccatcag agaaccattt
     5761 ggtgcacata taatagaaat ggtcgctggt ttgcaagtat ttccagtcga gctgaatctg
     5821 ctggtcggga gtgtttttca ccaaatattc caggtcatag agtttgttga aagcatcgtt
     5881 ctgcaggtcg ttgccgagcc aggccgtgag atcccgttct tcgtcagccc atgaaatggg
     5941 attcggcaca tatatggccg cccggggttg caatttttcc gagagctcag cgggtgtgta
     6001 aaaaccatag tttgaccggg caaacactgt ggcaggcagg gttttcataa attcaaagat
     6061 accggtttcg ggccattggt gttcgccgaa agtttcgtaa tccataaaca ggttgatggt
     6121 ttcctggtcg gaatcaattt cctgaatcca atttacaaac ttttccgtgg tcagtggcca
     6181 gccattccag ctttgttgcg aaaacctaaa agcaatatca tcgctcaggc ggaaattctt
     6241 cagcaaaagt tttagttttg gattgataga gttacaatac aggtagttcg gacttttcca
     6301 ccccataata tgtttggcgc cttcggtaag catcgttctg taacccatgt tggccacggc
     6361 agcgccaata gtgtcggaat aaataagctc ggtattgcgg aaagtggtcg gctcttgttt
     6421 aaaataccgt ttcaccaatt caacctgtga tgatacctgc cggttgaact cttcatcgct
     6481 tttcaatgag gataaggaat gtgaaaaggt ttctgccagg aattccacac aaccggtctc
     6541 ggcaagtttt atataacttt ccagcacatc gggagcgtaa cgttcaaact gatcgatgac
     6601 ggttcccgac aaagaaaaac tcactttaaa ggccgaacca tactctttga taagatcaag
     6661 cagcaggttg ttcatcggca gataacattt atccgcaatc cgtcgcatta tatagcggtt
     6721 gcggtattca tcaaaataaa aatggtttgc gcctatatcg aaaaaacggt atgttttcag
     6781 acgaaatggc tgatgaacct ggaaataaaa acaaatagat ctcattatcg tataggttaa
     6841 tttttaaata ttactttatt gtacacatcc ctgattttgc tggaagcgct ttcccatttg
     6901 aggttctcta cctcttcctt gccgtacttg cggaacatca ccgacagggc atcatagtgc
     6961 agcaggccat agatggcgtc ggccatcgca tcaatatccc agaaatcaat tttgagggca
     7021 tatttcaaca cctccgagac tcccgactgt tttgagataa ccacggggac gttggagcgc
     7081 atggcctcga ggggcgaaat gccaaaaggt tcggacacgg agggcatcac atacacatcg
     7141 ctcagcaaaa acattttatc gacctcggcg ccttgtaaaa acccagtgaa atgaaaatgg
     7201 gtggagatgc ccagttgggc tacccgcagt atcatccggt tgagcatatc ccctgaaccg
     7261 gccatgacaa agcgcacatt tttatcttta tccagcaccc gtttggctgc ttccacgaaa
     7321 taatcagggc ctttctgaaa cgtgatccgg cccagaaaag taaccacttt ttctttcaca
     7381 tttctttcaa tcaccaggtc gctgtctcct gtattgtgtt ccacagcgtt atgaacagta
     7441 aacaccttat cgggatttat cccatatttg ttgattacaa tatcgcgggt aaaattgctg
     7501 accgtgatga cggcgtcggc gatctccatg cccttgcgtt caatatcata tacattctgg
     7561 ttgatatgat cgcccgaacg gtcaaattcg gtagcatgca tgtgcaccac caggggcttg
     7621 ccgctgattt ttttggctgc aatgccggct ggataggtca gccagtcgtg ggcatgaata
     7681 acatcaaact gttgcgaacc ggcaagagtg gatgccacca acgcataacg ggtaacttcc
     7741 tccatgaggt ttttgccgta tttgccagag aatgtgtatt gttgggaaaa cacgctgctg
     7801 tgctcctcgg ttttgtcaat agtgttcaca gcagtcattt tttgaaattc ctcttcaccg
     7861 acataaggaa tgaggttgga gccaatttca atataagtaa gttgtttcca gtattcctgg
     7921 tacattttat tggtaaagtc aatacgcaca tcactggcat tgatgagcct tacggcttgc
     7981 tgatcttcgt cgccatagga cttgggaacc acaaacagca cttccacacc atttttgagc
     8041 agtcctttgg tcagcccgaa acaggcagta ccgaggcctc cggtgatatg agggggaaat
     8101 tcccagccaa acattaatac tttcatttta tcttatcaat tttttatagt ctgctgactg
     8161 ccacaggctg tcagtaaaat tttactaaat cattttacgc tactgtattt ttcaatcagg
     8221 tagtcaattc gtaacaattc agccacactc caggcctgcg aaggcgcacc accgggcaaa
     8281 taaggcgggt cgccgtcgta caattcggaa atagtgccaa taccatgttc catcatgacc
     8341 ggctcaaaac tttcatacaa ctgcttgata tgtggcaagc catagttggg atgaattttc
     8401 agatacgctt cagcatagtg accaagcaac cagggccagg caataccctg atgataagcg
     8461 gcatctcttt catggatgcc gcctttataa atgcctttat aatcgggcga cttgggcgac
     8521 aacgtacgaa taccgcgccg tgtcagcaat tcgcgttcgg tgatttcaag aatcagttcg
     8581 cgtatctgtt cgctgacagg gctataaggc agcgaagcag ccaacaactg gttgggccgt
     8641 accgaccagt ctttataatc gccgtttaca taatcgcaga ggtagccttt atctttgttc
     8701 cagaaagtac ctttgaaagc ggccggaatt ttacatgcga tttcatccca ttctttggca
     8761 aaatcctgat caccagccag ttttgccacc tcgatggcaa acttcacggc attgtaccac
     8821 aaggcattga tttcaacagc caggccaatg cggggcgtaa ccggtttgcc atccactatg
     8881 gcatccatcc aggtgagcgc ttttccttct atgccggcat aaatcaggcc gttgtcctgc
     8941 attttaatgt tatattcaca gccattccgg tatgcattaa gaatgagttt cattttttcg
     9001 ccgtactcct tccatatcat ggattgcgtg cccgtataaa tagcatattg ctgcaaggcc
     9061 caaaaaaacc acaagggcgc atccacagaa ttgtaggcag aattctggcc gcatcctacg
     9121 ttgggcaaca gtccgccttt taattcggca atcatattat cgatgatggc cttgcatttt
     9181 tcagggtcat taatagctaa agtcaggccc ggcaatgcaa taaaggtgtc tcttccccaa
     9241 cggccaaacc atggatagcc ggcaattatc tccactttgt tgtttcgggt aacaaaaaat
     9301 tgtaatccgg aatttatcag gcagtttttg taattatccc tgggaattct tttttttgtt
     9361 tcggaagaat aaatctgttt gagtttggag ccatctattt cgttcagccc ggcagtaaat
     9421 accacacttt cacctttgct cataggcagt tcaaaataac caggtacaaa aacatcttcc
     9481 tggtagtcgt atccacgttc tttttcttca acatattcaa tgttgtagtt ccagtgagga
     9541 acgtgggtat attgaatttt tttcgaacat tgcaggaata aagtcgagta tcccggatac
     9601 atccttattt ttataccgtt ttcaaccggt tcggcatgcg tatcggcatt agggttggcc
     9661 ttggttaagg catgaatatt ccggaaagcc aaaaaaggct gcaagcggat aactgttggt
     9721 gaatgagcat cttcgagggt atatttgatc aataggcggt cgctctcaat acaaaacatg
     9781 atttctttag taaatatcac accgccaaca cgatacgtaa attttggcat aggctccatc
     9841 tcaagaccgc tgatatactt atgccctttg ggatttaata caccatcagg
//
LOCUS       DAOSFP010000031         9862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5851_length_9862_cov_4.561742, whole genome shotgun sequence.
ACCESSION   DAOSFP010000031 DAOSFP010000000
VERSION     DAOSFP010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5851_length_9862_cov_4.561742"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..936)
                     /locus_tag="PLI16_01735"
     CDS             complement(133..936)
                     /locus_tag="PLI16_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOH83310.1"
                     /translation="MKKSTFFFIILLIAAFPACKNAEKATTTNFSGNNDYMEMAVLYH
                     QQSAEIKALYYQSFRLARLMFEQDLANAGVVKKRAVVVDIDETMLDNSPYQAKCILEQ
                     FSFPLHWDEWCNKGVADALPGTIDFLKYVAANGGEVFYVSNRTQDQYEGTLRNLKEKG
                     FPFADNEHLMLKTASTTKELYRQKIAEKYHISLLIGDNLSDFVSGFEKQSTLAREQKV
                     DSLQNEFGKRFIILPNAIYGDWESALYHHNYKRTEKQKDSCRKAGLKSF"
     gene            complement(1065..2555)
                     /locus_tag="PLI16_01740"
     CDS             complement(1065..2555)
                     /locus_tag="PLI16_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH83311.1"
                     /translation="MKPLVFCRKIVQLVPIALFIIVVGCRPVREAPKALPETKTLEIL
                     AVNDMHAAMDNFPRFAFMVDSLRAIYPNMLLVSAGDNQTGNPVNDQYHEKGMPMISLM
                     NAVKFDISAVGNHEFDSKPQGFAKNTQKATFSFVATNTVLPEGGAYRIKPYAVIRAKN
                     GVRVAFVSLLNINEGGIPDCHPDNVKEFSFRDPYVVAKETLYLADSSQVFVFLNHMGF
                     EEDVALANQLPAGSADLIIGGHSHTRVDKEQLHNGVLITQAERKLKFATLIKLTLKDN
                     QVETKSMQLLTVGKNGKERPDIRAMVNQFGNNPQLKETIAEATADFTSYEEVGYLMVD
                     GIRAASGAEIALANPGGVRIDHLDKGSVSVMDVYTMDPFGNEMVLFKLTGHELKALHL
                     AAFTIDDSSAIYPSGIKTKYLLTETGALADVQFFTNDGKPLEMDKTYTVVMNNYMASV
                     YRYEHKDNGQGLFRTTAEGIIDYLKNLKKIPDYRGEKRVELIKKGQ"
     gene            complement(2560..2760)
                     /locus_tag="PLI16_01745"
     CDS             complement(2560..2760)
                     /locus_tag="PLI16_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83312.1"
                     /translation="MRIRINRTDLELFGGATVADALRRYYVLHKKKFPEIFPAVTDRH
                     GNYVAPDGSLSEGNQLYLKTKK"
     gene            complement(3041..6571)
                     /gene="nifJ"
                     /locus_tag="PLI16_01750"
     CDS             complement(3041..6571)
                     /gene="nifJ"
                     /locus_tag="PLI16_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOH83313.1"
                     /translation="MEKKSKFITCDGNYAAAYIAYMFTEVACIYPITPSSNMAENIDE
                     WAAAGKKNIFGETVSLSEMQSEAGAAGALHGALQAGTLAATYTASQGLLLMIPNMYKI
                     AGELLPGVLHVSARSLAAQALSIFGDHSDVMATRNTGFAMLATGSVQEIMDIAGVAHF
                     TALKSRVPFLHFFDGFRTSHEIQKVEAPAMEDMEKLLDKQALKEFRQRALNPEHPVTR
                     GTAQNPDIYFQTREVANKFYEAVPDMVEHYMQEMQKITGREYHPFTYYGAPDAENVIV
                     AMGSITETIKEVIDHLVAKGEKVGLISVHLYRPFSEKYFMNVYPKTAKRVTVLDRTKE
                     PGANGDPLYLDVRDLFYGKPGAPMIVGGRYGLSSKDTTPAMMLSVFENMKMNEPKNQF
                     TVGIVDDVTFKSLPLLPEISVAPEGTYEAKFYGLGSDGTVGANKNSIKIISETTDKYC
                     QAYFAYDSKKSGGITVSHLRFGDKAIRSPYLVNTPNFVACHVPAYLEKYDMLKGLKNN
                     GTFLLNSIWDVEETKAHLPNHIKKYLAANNIKFYIINATKIAEEIGLGTRTNTIMQTA
                     FFKVSNVIPFDLAVTQMKKAIEKTYGRKGEDVVKKNIASIDDAIKGVVEVPVPADWKN
                     LPDDVVKKNNDLPDFIKNVFNPINAQKGDDLPVSAFLGREDGTFPSGTTRFEKRGIAV
                     NVPEWVPENCIQCNQCAFVCPHAAIRPFLLNDEDVKNAPAGTKTLKAVGKELEGLQFR
                     MQVSVLDCTGCENCADVCPAKTKALVMKPLGTQQAEIERFTYMDNKVGYKSSSVDIFK
                     IFKNSQFAQPLFEFSGACAGCGETPYIKLVTQLFGERMIVANATGCSSIYGGSAPSTP
                     YSIAKSGKGVAWGNSLFEDNAEYGYGIESGVRKMRQRIQRLMKTGMDSDLSAEIKDAF
                     KAWLEGMNNAEASKTASEKVLALIEKSEHPLAKQIVELKDYLVKKSIWIFGGDGWAYD
                     IGYGGLDHVLASGEDVNVLVLDTEVYSNTGGQASKSTPIAAVAKFAAAGKRIRKKDLG
                     MMAMSYGYVYVAQVAIGASYAQYFKAVKEAEAYPGPSLIIAYAPCINHGLRAGMGKSQ
                     QEAERAVEAGYWHLYRYNPMLEKEGKNPFTLDSKEPKWEEFQNFLAGEVRYTSLKSTF
                     PEEAEALFKAAEENARWRYKTYVMKSQMQY"
     gene            complement(6862..7074)
                     /locus_tag="PLI16_01755"
     CDS             complement(6862..7074)
                     /locus_tag="PLI16_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83314.1"
                     /translation="MKTIKVFCAIFSIAALISCHPSQEEIDKQEKEKDSLMEIERDKA
                     LNNADKLLEQMDSMDATGDTITPEKK"
     gene            complement(7207..8016)
                     /locus_tag="PLI16_01760"
     CDS             complement(7207..8016)
                     /locus_tag="PLI16_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH83315.1"
                     /translation="MKKILIIVGLLIILYSLICLLVYSFQKYVVFKPQKGAVPPPDDL
                     EIQELKLQTSDGESLHAWWMPTDDAQYTILFFHGNAGNISRGEKRMRLFREMGCNALT
                     IDYRGYGISSGSIKKEEDIYEDSRTAWQYLIKEQGIPEEKIIIWGWSMGGAVAVNLAM
                     QKKCHALVMESTFYSLYDMAGFFYRFLPMSWLMKYHFTSGEKLSEISVPVLFAHSPAD
                     ETVPYINGMRLYDDFSGTKKFIKLTGDHNHGIFDTQDKFVPQAKQFLKLSN"
     gene            complement(8082..8927)
                     /locus_tag="PLI16_01765"
     CDS             complement(8082..8927)
                     /locus_tag="PLI16_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83316.1"
                     /translation="MKALKVLSVCLLVIFTGSIIFSTSCKKEEDDDNQYSYRAAQDNN
                     RAETAFNLSASEIQKAARQVGSKSTNDTIQGCPVIYVTGGWPKTVTLDYGTGCTGTDG
                     ITRKGQIVTVMTGLYVDSSSVMTSTFQNFSETINGIEHNITGTQIITNLGHNQAGHPY
                     FSVDVQNASVSYTEGTIHWTSQRQNEWIAGYDTYMNPWDDEYYVTGSADGTDINGAGF
                     SVNITSPLYCKFCMSIFRWVVASGTVDVVNPGYPTITVDYGNGDCDLVVYIVINGTTY
                     TVVLS"
     gene            complement(9022..>9862)
                     /gene="meaB"
                     /locus_tag="PLI16_01770"
     CDS             complement(9022..>9862)
                     /gene="meaB"
                     /locus_tag="PLI16_01770"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOH83317.1"
                     /translation="LRIGFTGVPGVGKSTLIEALGMYLTKRKYKLAVLAIDPSSYRTK
                     GSILGDKTRMEELAADPNAFIRPSPSAGTLGGVARKTWETIILCEAAGFNIIFVETVG
                     VGQSEVLVHSMVDFFLLLMLAGAGDELQGIKRGIMEMADAIVINKADGDNIARADIAR
                     MEYQKALHLFPTSLSGWEPKVTTCSARENKGIKELWKIIEEYQSYNKNNGCFFGKRTE
                     QSLARMHYAIKEQLFTDFYKNKKIETKLKHIESKIANNTVSSYIAARELLELYYHSMN
                     NPD"
BASE COUNT         2489 a         2127 c         2193 g         3053 t
ORIGIN      
        1 aatttcattt ttttatttaa acctatgaat ttctaattca tagtggttta gtttttcttt
       61 ggtgacagaa gccggatatc gaaagaaatc cggctttttt tacgggctct tttcagcagt
      121 taaaaaaatt ttctaaaaac tcttcaggcc ggcttttcgg cagctgtctt tttgtttttc
      181 agtgcgtttg taattgtgat ggtatagtgc cgattcccag tcgccatata tggcattggg
      241 caatattata aagcgcttgc caaattcgtt ttgcaagctg tccacttttt gctccctggc
      301 taaggtcgat tgtttttcga aaccactgac aaaatccgat aagttatcgc caatcagcag
      361 gctgatgtga tatttttcgg ctattttctg ccggtataac tccttggtgg ttgaggctgt
      421 ttttagcata aggtgctcat tgtcggcaaa tgggaatcct ttttctttca ggtttctcag
      481 cgtcccttcg tattgatcct gcgtgcggtt cgaaacataa aacacttccc ctccattggc
      541 ggcaacatac ttcaaaaagt ctatagttcc cggcaaggca tcggcgacac ccttgttgca
      601 ccattcgtcc cagtgcagcg gaaacgaaaa ctgttcaaga atacatttgg cctggtaagg
      661 actgttgtcg agcatggttt catcaatatc aaccacaaca gctctttttt tcactactcc
      721 agcgttggct aaatcctgct caaacatcag gcgggccaag cggaacgact gataatacag
      781 ggcttttatt tcagcggact gctggtggta caaaaccgcc atttccatat aatcattatt
      841 ccccgaaaaa ttcgtcgtgg tggctttttc agcattttta catgccggga aagcagctat
      901 cagtagtatg ataaaaaaga aagtcgattt tttcattttt gtttgaattt aataatacgc
      961 tttgatggaa aaactacgaa tcaaattatc gataaaaata gtagttataa taacaaaaaa
     1021 tccatttttc tccatttttt ggtgtgtaaa ctacacacca tgaactattg tcctttttta
     1081 atgagctcaa cgcgtttttc tcctctgtaa tcgggtattt tctttaggtt tttcaaataa
     1141 tctattatcc cttctgcggt agttctgaac aacccttgtc cgttgtcttt gtgctcgtaa
     1201 cggtacaccg atgccatata gttattcatg accaccgtgt aggtcttgtc catttccagc
     1261 ggtttgccgt cgttggtaaa aaactgcacg tcggccaggg cgccggtttc ggtaagcagg
     1321 tattttgttt ttatccctga aggataaatg gcgctgctgt cgtcgatggt gaaggcggcc
     1381 agatgcagtg ccttcagttc gtgtcctgtg agtttgaata aaaccatttc gttgccaaaa
     1441 ggatccatgg tgtacacatc catcacgctg acactgcctt tgtccagatg gtcgatacgc
     1501 acgccgccgg ggttggccag ggctatttcg gcgcctgagg cagcccgtat gccgtccacc
     1561 atcaggtagc ccacttcttc gtacgatgta aaatcggcag tggcttcggc aatggtttct
     1621 tttagctgtg gattatttcc aaattgatta accattgcac gaatgtccgg gcgttctttg
     1681 ccgtttttgc cgacagtcag cagttgcatg cttttagttt ccacctgatt atcttttagt
     1741 gtgagtttta tcagcgtggc aaattttagt ttgcgttccg cctgcgtaat cagcacgcca
     1801 ttgtgcagtt gttctttatc aacgcgcgta tgcgaatgac cgccaataat caggtcggcg
     1861 cttccggcag gcaattggtt ggctagtgcc acatcctctt caaagcccat gtgattgagg
     1921 aaaacaaaaa cctgcgaact gtcggccaga taaagagttt ctttggcgac aacataaggg
     1981 tctctgaaac taaactcttt aacattatca ggatgacagt cggggatgcc gccttcgttg
     2041 atattcaaca gtgaaacaaa agctacgcga acaccatttt ttgctcttat cacggcatac
     2101 ggtttaatgc ggtatgcacc accttcgggc agtacggtat tggtggcgac aaaactaaaa
     2161 gttgcctttt gggtgttttt tgcaaacccc tgcggcttgg aatcgaactc gtggtttcct
     2221 actgccgaga tgtcaaattt tacggcattc ataagcgata tcatgggcat ccctttttcg
     2281 tggtactggt cgtttacggg gtttcctgtc tggttgtcac cggccgacac cagcagcatg
     2341 ttgggataga tcgccctgag actgtccacc atgaatgcaa agcgcggaaa attgtccatg
     2401 gccgcatgca tatcgtttac ggcaagtatt tccagagttt ttgtttcggg gagggctttc
     2461 ggcgcctcgc gcacggggcg acagccaaca acaatgataa aaagcgctat aggaacaagc
     2521 tgcacaattt ttcgacagaa aactaatggt ttcataaggt tatttttttg tttttaagta
     2581 cagttgattg ccttcggaca agctgccgtc gggggctaca taatttccat gacggtcggt
     2641 aaccgccgga aaaatttcag gaaatttttt cttatgcagg acataatatc ttcgtaaagc
     2701 gtcggctacg gttgccccgc caaacagttc gagatcggtt ctgttgatgc gtattctcat
     2761 cgtcagcaaa ttgtttgcaa gatactgatt ttgcatttat gccaaataga aattaaaaat
     2821 atgcatggat tttatggtat aatgccgatg attaaaataa atagtgcaaa cgagtgatgc
     2881 gaagtttgga ctatattatt tttccaatcg gtattattta ttatcgatag gatcatattt
     2941 ttatgagtca gttgcaaatg catgtcatcc attgttggaa acagcaaaaa aaatccaggt
     3001 ggttattgcc tggatttttt ttgtgataga gaatactctt ttagtattgc atttgcgatt
     3061 tcatcacgta agtcttatac ctccacctgg cgttttcttc agccgctttg aacaaagcct
     3121 ctgcttcttc ggggaaggtg cttttcaaag aggtatagcg gacttctcct gccaggaaat
     3181 tctggaattc ttcccatttt ggctctttcg agtcgagtgt gaaaggattt tttccttctt
     3241 tttccaacat ggggttgtag cggtacaggt gccaatatcc tgcctctaca gcacgttcgg
     3301 cttcctgctg ggattttccc ataccggcac gcagtccgtg gttgatacag ggagcatacg
     3361 caataatcaa cgagggtccg gggtatgctt ctgcttcttt tacggctttg aaatactgcg
     3421 cataactggc tccgatagcc acctgggcaa catatacata cccataagac attgccatca
     3481 tacctaagtc ttttttgcgg atgcgtttac ctgccgcggc aaattttgcc actgcggcaa
     3541 taggtgttga tttagaggcc tgtccgcctg tattggaata tacttcagta tcgagaacaa
     3601 gcacatttac atcttcgcct gaagccagca catggtcaag tccgccgtaa ccgatatcat
     3661 aagcccagcc gtcgccgccg aatatccaga tagatttttt taccaggtaa tctttcagtt
     3721 caacaatttg ttttgccagc ggatgttccg acttttcaat cagggccaac actttttcgg
     3781 atgctgtttt tgaggcttct gcgttgttca tgccttcgag ccatgccttg aaagcgtctt
     3841 ttatttcagc ggacaaatca gaatccatac cggttttcat cagtctttgg atacgttgac
     3901 gcatttttct cacaccgctt tcgatgccat aaccatattc ggcattatct tcaaacagcg
     3961 agttgcccca ggcaacaccc ttgcccgatt ttgcaatgga ataaggtgtg gaaggtgccg
     4021 atccgccata gatggaagaa caaccggtag cattcgccac gatcattctt tcgccgaaga
     4081 gttgggtgac caatttaatg tagggagttt caccacaacc ggcgcaggcg cccgaaaatt
     4141 caaacaatgg ctgagcaaac tggctgtttt taaatatctt aaagatgtca acactggatg
     4201 atttgtaacc cactttgtta tccatatagg taaagcgttc aatttctgcc tgctgcgtac
     4261 cgaggggttt catcacaagt gctttggttt tggcaggaca aacatcggcg cagttttcgc
     4321 atccggtaca atccagaaca cttacctgca tacggaactg caggccttcc aactctttac
     4381 caacggcttt taatgttttg gtgccggcag gtgcattttt cacatcttcg tcgttgagca
     4441 ggaacggacg gatagcagcg tgagggcaaa caaaagcgca ctggttgcac tgtatgcagt
     4501 tttcgggtac ccattcggga acatttacgg cgatacctct tttttcgaag cgggtagtgc
     4561 ctgaggggaa ggtgccgtct tctctgccca ggaaagcgct cacgggcagg tcatcgcctt
     4621 tttgtgcgtt gatgggattg aagacatttt taatgaaatc cggcaggtcg ttattttttt
     4681 tgacaacatc gtcggggagg tttttccagt cggcaggcac cggaacttct accactccct
     4741 tgatagcatc gtcaatagag gcgatgttct tttttaccac atcttcacct ttacggccat
     4801 aggtcttttc gatagctttt ttcatctgtg taacagccag gtcgaaagga atcacgttgg
     4861 agactttgaa gaaagcagtt tgcataatgg tattggtacg ggtaccgaga ccaatttctt
     4921 cggctatttt ggtggcattg atgatgtaaa acttaatgtt gttggcggcc aggtattttt
     4981 ttatatgatt gggcaggtga gccttggttt cttcaacatc ccagatgctg ttcagcagga
     5041 aggtgccgtt gttcttaagt cctttgagca tatcgtattt ctcaagatat gcaggaacgt
     5101 ggcaggccac gaagttgggt gtatttacga gataaggcga tctgatggcc ttgtcgccaa
     5161 aacgcaggtg ggatacggtg ataccgcctg attttttgga gtcgtaggcg aaataggcct
     5221 ggcaatattt atctgtggtt tcgctgataa tttttatcga atttttgtta gcgcctaccg
     5281 taccgtcgga ccccagtcca taaaatttgg cttcgtaggt tccttcggga gccacgctga
     5341 tctcgggtaa caagggaagc gatttaaagg taacatcatc aacaatacct acggtgaatt
     5401 ggtttttcgg ttcgttcatt ttcatgtttt cgaacaccga caacatcatc gcgggtgtgg
     5461 tgtctttgga gcttaatccg tagcgtccgc ccactatcat gggtgcaccg ggtttgccat
     5521 agaagaggtc gcgtacatca agatacaaag gatcgccgtt ggcgccgggt tctttggtac
     5581 ggtcgaggac tgtgactctt ttggcggttt tcgggtaaac attcataaaa tatttttccg
     5641 agaaaggacg gtacagatga accgaaataa gacctacttt ttctccttta gctaccaggt
     5701 ggtcaatcac ttcttttatt gtttctgtga tagaacccat agcaacaata acgttttcag
     5761 catcgggagc gccataatag gtaaagggat ggtattccct gccggtaatt ttctgcattt
     5821 cctgcatata atgctcaacc atatcgggaa cggcttcgta aaatttgtta gcgacttcgc
     5881 gggtctggaa ataaatatcc gggttttgtg cagtacccct tgttacagga tgttcagggt
     5941 tcagtgctct ttggcggaat tcttttaatg cttgtttgtc aaggagtttt tccatgtctt
     6001 ccattgccgg agcttccact ttttgaattt cgtgtgaagt acggaatcca tcgaagaaat
     6061 gcaggaaagg aactcttgat ttaagagctg taaaatgtgc aacgccggcg atgtccataa
     6121 tttcctgaac gcttccggtg gccagcatgg caaaacccgt gttgcgggta gccatcacat
     6181 cgctatggtc gccaaaaatt gacaaggcct gtgcagccag actgcgggca gacacatgaa
     6241 gtacacccgg taataattca ccggcgattt tatacatatt cggaatcatt aacagcagtc
     6301 cttgtgaggc tgtgtatgtt gccgccagcg tacctgcctg taaagccccg tgcaatgcgc
     6361 ctgcagcgcc agcttcggac tgcatctctg ataaacttac tgtttcgcca aagatattct
     6421 tttttcccgc ggctgcccat tcatcaatgt tttctgccat attagacgat ggcgtaatag
     6481 gataaatgca tgctacttcg gtaaacatat aagcaatata tgctgctgca taattaccgt
     6541 cacatgtgat aaattttgat tttttttcca tatcaaattt tcttgtttaa gttgctgata
     6601 atatgattaa taactattta ctttcgatca ggttgcgaaa ttaatgaatt attttgaaaa
     6661 tattgccctt ttggttataa aaaaagccct gataacaaat tataaatctg attatttatg
     6721 atgaaaggtt ctatgtttca tgttattagg gggagaaacc gccaattatt tattaatatt
     6781 cacaaaaaca agatattaca cgctctaata actttagcat aaaagcatga aaaatattaa
     6841 aattgttttt gtggccggaa tttatttttt ttcgggagtt atggtatctc cggtggcatc
     6901 cattgagtcc atttgttcca aaagtttgtc ggcattattt agagctttgt ctctttcaat
     6961 ttccatcaga gaatctttct ccttttcttg tttatcaatt tcttcctggc tggggtggca
     7021 ggatatcagt gcggctattg aaaaaatagc acaaaatact ttgatagttt tcatgtttgt
     7081 cttacgatta gttttaaaca gtaattttct ttttcaaaat gtatcggtag aaaaagcttt
     7141 tattaggttt tctgtaggat gattcagctt tacatccttg tttaattatg gtacaaaact
     7201 aacaattcag tttgataatt tcagaaattg ttttgcctga ggaacgaatt tatcctgagt
     7261 gtcaaaaatg ccgtgattat gatcacctgt cagtttaata aactttttgg tgccggaaaa
     7321 atcgtcatat agccgcatgc catttatgta ggggactgtc tcgtcggcgg gactatgagc
     7381 aaatagtacc ggcacggata tttccgataa tttttcgccc gaagtaaaat gatacttcat
     7441 cagccacgac ataggcaaaa aacgataaaa aaagcctgcc atatcgtaaa gactgtaaaa
     7501 ggttgattcc atgaccagcg catgacattt tttttgcatg gccaggttca ccgccacggc
     7561 tccccccatc gaccatcccc agataataat tttttcttcg ggaatgccct gttcttttat
     7621 aaggtactgc caagccgtac gggaatcttc ataaatatcc tcttcttttt ttatactgcc
     7681 gctgctgatg ccataacctc tgtaatcaat ggtcagggca ttgcagccca tttccctgaa
     7741 caagcgcata cgtttttcac ctcttgaaat atttccggca ttaccgtgaa aaaaaagaat
     7801 ggtgtattgg gcgtcatccg tgggcatcca ccaggcatga agagattccc catcggaagt
     7861 ttgcagcttt aattcttgta tttccaaatc atccggggga ggcacagcgc ctttttgtgg
     7921 tttgaaaacc acatactttt gaaatgaata aaccaagagg caaatcaacg aatacaagat
     7981 gatgagtaag ccaacgatta tcaggatttt tttcattttt tcaggcatta aaaaagcctg
     8041 ccacagagaa tcgtaacagg ctttgtttct taaaaaaatc tttatgaaag cactacggta
     8101 taagtcgtac cgtttatgac aatataaaca accaggtcgc aatcgccgtt gccataatcc
     8161 accgtaatgg taggatatcc cgggttgaca acgtccacgg ttccggaggc tactacccag
     8221 cggaaaatgg acatgcagaa tttacaatag agcggtgaag tgatatttac gctgaatccc
     8281 gctccgttga tatcggtacc gtcagccgag ccggtaacat agtattcgtc atcccaggga
     8341 ttcatatagg tgtcgtatcc tgcgatccat tcgttttggc gctgagaggt ccagtggatt
     8401 gttccttcag tataagaaac actggcattc tgaacatcaa cagaaaaata cggatgtccg
     8461 gcctggttgt gaccaagatt ggttatgatc tgagtgccgg tgatattatg ttctattcca
     8521 tttatagttt cagaaaaatt ctgaaaggtt gaggtcatta ccgaagaaga atctacataa
     8581 aggccggtca tcacggtaac tatctgtcct ttgcgggtaa tgccgtcggt gccggtacat
     8641 cctgtgccgt aatccaaagt aactgttttg ggccagccgc cggtaacgta aataaccgga
     8701 caaccttgaa tagtgtcgtt agtgcttttg cttcccacct gccgggcagc tttttgaatt
     8761 tcggaggccg aaaggttaaa ggccgtttct gcacggttgt tgtcctgagc agcacgatag
     8821 ctgtattggt tatcatcatc ttcttccttc ttgcaggagg tggagaaaat tatactgccc
     8881 gtaaagatta ccaacaggca tacacttaaa acttttaaag ctttcataat ggttgttttt
     8941 taattaaaca atttacagga attatcaaat aaacgaattt tattacacaa ttatttgcta
     9001 tatgcagtgt ttttttgaaa attaatccgg gttattcatg gagtgataat aaagttcaag
     9061 tagttcacgt gctgcaatat aagagctgac agtgttattg gctattttac tttcgatatg
     9121 ttttagtttg gtttctattt ttttgttttt gtaaaaatcg gtaaaaagtt gttctttgat
     9181 ggcataatgc atgcgggcaa gggattgttc ggtccgttta ccaaaaaaac agccgttgtt
     9241 tttgttatag gactggtact cctcaatgat tttccataat tcttttattc ctttattttc
     9301 gcgtgccgag caggtggtaa ccttgggctc ccagcccgaa agcgacgtgg gaaaaagatg
     9361 cagcgctttt tgatattcca tgcgggcgat gtcggccctg gcaatattgt caccatcggc
     9421 tttgttaatc acaatggcat cggccatctc catgatgcct cgttttatgc cctgcagttc
     9481 gtcgccggcg ccggccagca tcagcagcag aaaaaagtca accatagagt gcaccagcac
     9541 ttccgactgt cccacgccca cggtttcaac aaaaattatg ttaaaccctg ctgcttcaca
     9601 caaaataatt gtctcccagg ttttgcgggc tacccctccc agggtgcctg ctgatggaga
     9661 cggacggatg aaggcattgg ggtcggctgc cagttcttcc atgcgggttt tgtcgcccag
     9721 gatactgcct ttggtgcggt agctgctggg gtcgatagcc agcacggcca gtttgtattt
     9781 acgttttgta agatacatcc caagtgcttc tatcagtgta cttttgccca caccggggac
     9841 cccggtaaaa cctatgcgca gc
//
LOCUS       DAOSFP010000032         9773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5922_length_9773_cov_4.263326, whole genome shotgun sequence.
ACCESSION   DAOSFP010000032 DAOSFP010000000
VERSION     DAOSFP010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5922_length_9773_cov_4.263326"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /gene="yidC"
                     /locus_tag="PLI16_01775"
     CDS             <1..1050
                     /gene="yidC"
                     /locus_tag="PLI16_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOH83318.1"
                     /translation="KQQFFSATLIAKESFKEATLETVQQKDTLFPGYLKTLTASLTFP
                     YENQSSQSIPMSIYLGPNKYKILKSYDLDMERQIPLGWGFFLMHWINRFAVIPVFNWL
                     ETYGINYGIIILILTILLKIVLFPIAYKTYLSSARMRVLKPEVDEIAQKFPKKEDAMK
                     KQQATMALYKKAGINPMAGCIPMLLQMPILIALFRFFPASIELRQQPFLWAEDLSSYD
                     SIWNFPGGFSIPFYGDHVSLFCLLMTISTILYTKLNNQMMGSTSQPGMKFIMYAMPVM
                     FLGFFNDFASGLSYYYLLANLITFLQMFLFRKFVNEEKIHARIQANKKKPVKVSGFQK
                     RLEEMAKKGGYPVKK"
     gene            1159..2112
                     /locus_tag="PLI16_01780"
     CDS             1159..2112
                     /locus_tag="PLI16_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH83319.1"
                     /translation="MNLNKKNPSTVLLGWAILLVLVLVWGSSFILMKRGLEHYSHTQL
                     ALMRISIACIFLIPFAIFSIKKINRKKLGYIALVGIIGNGIPAFLFAKAQTGIDSSLA
                     GILNSLTPLFTLIAGLLFFGYKARWINITGVFIGLAGTVGLMSISGNKTLDFNFRYGI
                     YIMVATVLYAINLNIVKKYLEDTDPVTITAFAFLIIGIPALIVLFFFTDFVLVLTTQA
                     GALEGLGYIAILGIFGSGLAVILHNKLIKTSGVLFAASVTYMIPVVAVLWGIADGEAF
                     EPVYLFWIALILLGIFMVNRLEGKNKLPKTAKEIKTENKHT"
     gene            2183..3325
                     /locus_tag="PLI16_01785"
     CDS             2183..3325
                     /locus_tag="PLI16_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HOH83320.1"
                     /translation="MQEYKICKRCIMDTTDPEIVFDENGYCNHCSKAIKLLNTPPVSL
                     NAEEKIKSLQALVEKIKSEGKNKKYDCIIGVSGGVDSTFAAYFVKKNGLNPLAVHLDN
                     GWNSRIAVTNIEKTLKTLNIDLYTHVIDWEEFKDLQLSFLKASTPDSEVPSDHAITAL
                     LYKVAAQHGVKYILAGTNITSESILPKAWSQGHSDWKYIHSLQKQFGTARLKTFPHYG
                     ILRLFYYIVIRKIKYISILNYTDYAKEEAKKLLISELGWEDYGGKHHESVYTHFYQSY
                     LLPKKFGFDKRRAHLSSLIMAGQITREEALEEMKKPAISDDMARTEEEYIINKFGLTR
                     ESYDRIMNTPPKSFHDYPSYFNSFYYKFFFKIYKALGALGVKKSDI"
     gene            complement(3341..4969)
                     /locus_tag="PLI16_01790"
     CDS             complement(3341..4969)
                     /locus_tag="PLI16_01790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83321.1"
                     /translation="MRYAIITPVFNEENSIKNLLQSVAGQTLKPVQWVIYDDGSTDQT
                     AAIITAFAADFPWIKLVQNKAKKSHMAGTNIARIFNNCYRNHIIPDIDFVVKLDADLS
                     LKPNYFEAVAAHFAADQKLAVCGGICLVEINGEWIREKITNKDHVRGAIKAYRKTFLD
                     HIGGLREIDGWDAVDEMLAKFYQYTILADQSLEVLHHRPTDRKTGHLKAHKMTGRGCY
                     FMGFNAIAAGISCSKRIKYKPMLIGPCVAYGTYVMMFFGKEKKAVTPEQGRFISKLVI
                     KNAMKKSFRMIDNKKYYEQYQWDVIDEAGLAGKIQKILETIPPEVVSIIDIGCGNGVI
                     TNELGKKYAMLGVDRSAHALEHVKTEKLQASSDSIPLPDASFDMVLSSEMLEHLDEET
                     LKKTIGEITRLSKKYILVTVPNNENLDKFKLHCPQCGKNYNHSYHLHSFTREKLGELF
                     SGCKISKTFYYGTPVRRYNKILAKLKIRLCPPASWFPYFAKKIKDEKTMCPFCEHEFV
                     HNYKSHPLAFLFDAFNMIISPKVPYWIFVLFEKE"
     gene            5165..6673
                     /locus_tag="PLI16_01795"
     CDS             5165..6673
                     /locus_tag="PLI16_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH83322.1"
                     /translation="MGIIKRQSIKSTIVIYCGMMLGYVNLLLLSPLILSPEQIGLTRV
                     LYDGSYMFAMLAQLGIWSVATKFYSYFRDKNKKDNGFLFYMVSIPAVGFLILISLLLI
                     FNEQFTGLFIQKSPLIVDYYYYFIPLIFIVMYYQVFETYSSLQYRIVVPNLLREVLNK
                     FMYLIIILLLLSQMINFKMFVNFYVLSYGVSLIVLILYLRSMHTLHFKPDFSFVSPEL
                     RKQMITYGVFVFFGSLGAFAVARMDVIMLSSLSGLYFAGIYSISYLLGNVIEVPRKAI
                     AQISVPIIADAWKNNDIDKIDEIYKKTSINQAIVAGLIFLVIWINIEAIFSLIPNSSV
                     YIAGKYVALFIGLARFIDAATGVNTEILMNSHKHYKFNLVLLFAMGLLTIVLNLILIP
                     ILNMYGACLSVLISILLYNIFKFVFIYKKWKIQPFTFNTLKVILLLIVLVFVSYLFPK
                     LHNPILQIIMVSVPVITVYGLACYWMKVSADANDLANLAINKIKRLLSPKNK"
     gene            6726..8228
                     /locus_tag="PLI16_01800"
     CDS             6726..8228
                     /locus_tag="PLI16_01800"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HOH83323.1"
                     /translation="MNKLLLLQKTFRHYACVALMTALSATSAIAQTDILFKEDFENYS
                     SEGAFGAVTAVNGWLQNSTDPNNAIRNLWCSYSGTNYCPSCTNPTAGVRTISGVRSAQ
                     ICYINASNTIAWDGNYYGVGTYNTATTTTDRHIFHSINSTGYQNIVLSFNWKCAGEIY
                     GGNPVDYGQAAYRPGNTGSWIKLTSGGYNNTGLLCNTGQMVNAASYNLPAACNNTTFQ
                     IAFFWNNDNIGGSGPAFIVDDILVTGTRTTPDYCRPYHQYGNCSPWNDCQYVGITNVS
                     CGSINNTTSIANFPPAYSDFTNISTNLIVNNTYNVGVTYSDNGSPVNYGKIALWIDFN
                     GDLDFLDANEFIGEQQATATGQVKNFGFTVPSDAFNGSVVMRVRCAYTDEGFAATDPC
                     SLRDYGETEDYMLNITNISTVIDLPENDQFTAMPNPATDEIYVKNNDLSDPITLIIIT
                     DITGKQLIQKDGLACHDERISLKQLNPGIYFLVIKTHNGHHSVIKIAVAR"
     gene            complement(8308..9081)
                     /locus_tag="PLI16_01805"
     CDS             complement(8308..9081)
                     /locus_tag="PLI16_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HOH83324.1"
                     /translation="MWRPLMLKIRVIPCLLLKNDGLVKSVKFKNPTYVGDPINAVKIF
                     NDKEVDELLFIDIEASKKGREPNYDLIARISCEAFMPFGYGGGITTVEQVKKLTRLGV
                     EKVAINAYARKNPDFITQASEVAGSSSVVVSIDIKKDLLGRLRVFDYALQKTTSLDPV
                     EYAQLMESKGAGEIFLNSVDRDGTFSGYDTELIKKVTQAVTVPVVAAGGAGHIRHFAD
                     AVKSGASAVAAGSFFIFYGPHRAVLITYPDKASLKEIFR"
     gene            complement(9059..>9773)
                     /gene="hisH"
                     /locus_tag="PLI16_01810"
     CDS             complement(9059..>9773)
                     /gene="hisH"
                     /locus_tag="PLI16_01810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOH83325.1"
                     /translation="SACCFCRSQRKEKFGTKTKKVIIITPQQHFMIVVVDYGMGNIGS
                     IVNMIKKAGGESLVTSDPELVAGAGKIIIPGVGAFDNGMKNLREKGLIDVLNKKVLEE
                     QVPVLGICLGMQLMARSSEEGVLPGLGWIDAECKKFRFDDNNRLLRIPHMGWNDVVVK
                     NKEGIFRNMYDEASFYFVHSYHVECRHPEDIAATTMYGVEFTSSLQRSNIFATQFHPE
                     KSHKFGLLLMKNFVEAPYA"
BASE COUNT         2739 a         2246 c         1963 g         2825 t
ORIGIN      
        1 aaacaacagt ttttctctgc tacactcata gccaaagaaa gctttaaaga agccaccctg
       61 gaaaccgtcc agcaaaaaga taccttattc ccaggttacc tgaaaacatt aactgcctcg
      121 cttactttcc cttacgagaa ccaatccagt cagagcattc ccatgtcgat atacctggga
      181 cccaacaaat acaagatact caaatcgtat gatctcgata tggaacgcca gattcctctt
      241 ggctggggat ttttccttat gcactggatc aacaggtttg cggttatccc ggtgttcaac
      301 tggttggaaa cctatgggat aaactacgga attattatcc tcatcctgac catcctgctt
      361 aagattgtcc tgttccctat agcctataaa acttacttat cgtcggcacg tatgagggtg
      421 cttaaacccg aggtggacga gattgctcag aaatttccca aaaaggaaga tgccatgaaa
      481 aaacaacagg ccactatggc gctgtataaa aaagccggca tcaaccccat ggcgggctgc
      541 atacccatgc tgctgcagat gcccatcctt atcgccctct tccgcttttt ccctgcctcc
      601 atcgaactgc ggcagcaacc ctttctatgg gcagaagatt tatcgtctta tgactccatc
      661 tggaacttcc ccggggggtt ctccatacct ttttatggcg accatgtgag tttgttctgc
      721 ctgctgatga ccatttccac cattttatat accaagctca acaaccagat gatgggaagt
      781 acatcccagc cgggcatgaa atttattatg tacgccatgc cggtgatgtt cctgggattc
      841 ttcaacgact ttgcctccgg tttgagttat tattacctgc tggccaacct catcaccttc
      901 ctgcagatgt tccttttcag aaaatttgtg aacgaagaaa aaatccatgc gcgtattcag
      961 gccaacaaga aaaaaccggt gaaagtctcc ggcttccaga aacgacttga agaaatggct
     1021 aaaaaaggag gttatcctgt aaaaaaataa tccctccggg aattattaca acatcatttt
     1081 ttactattca tagcggaata tatcaaccga aatgcagcgc gatttttttg accatctctt
     1141 atagtccgga tacatacaat gaatttgaat aaaaaaaatc ccagtacagt actgttgggc
     1201 tgggctattt tgctggtact tgtcctggtg tggggcagtt catttatcct gatgaaacgc
     1261 ggcctggaac actactccca cacccagctg gcgctcatgc gcatcagcat tgcctgtatt
     1321 tttctcatcc cttttgccat cttttctata aaaaaaatta atagaaaaaa gctcggttat
     1381 atagctttgg tgggcattat aggcaacggc atcccggcct ttctgtttgc caaagcccaa
     1441 accggcattg acagttcgct ggcaggcatt ctcaactcat tgacaccctt gtttaccctg
     1501 atagccggcc ttcttttttt cggctacaaa gcaagatgga taaacatcac gggcgtattt
     1561 atcggactgg ccggcaccgt gggcctgatg agcatcagtg gaaacaaaac gctggatttt
     1621 aatttcagat atggtattta catcatggtg gccaccgtgt tatatgcgat caaccttaat
     1681 atcgtaaaaa aatatctgga agacaccgat ccggtaacca tcacggcctt cgccttcctg
     1741 ataattggca tccccgcttt aatagtattg ttttttttca cggatttcgt acttgtgcta
     1801 acaacacaag ccggagcatt agagggatta ggatatattg ccatactcgg gattttcgga
     1861 tcgggattag ctgttattct tcataacaaa ctgatcaaaa cttccggtgt tttgtttgcc
     1921 gcctcagtta cctatatgat acccgtagtg gccgtgctgt ggggtatagc cgatggcgag
     1981 gcctttgagc cggtatatct tttctggata gcacttatcc tgcttgggat ttttatggtt
     2041 aaccgtcttg agggcaaaaa caaacttccc aaaacggcca aagaaattaa aacagaaaat
     2101 aagcatacat aaaaacagtt caccggtaga aaaccaaaaa attttacctt attttgcacc
     2161 ttacaaaaaa tttttcaatt ctatgcagga atataagatt tgcaaaagat gtatcatgga
     2221 tactaccgat ccggaaatag tcttcgatga aaatggctat tgcaaccatt gcagcaaagc
     2281 cattaaactc ctcaacaccc cacccgtttc tctcaatgca gaagaaaaaa taaaaagcct
     2341 tcaggcgttg gtagaaaaaa taaaatcgga aggcaaaaac aaaaaatacg actgtatcat
     2401 cggcgtgagc ggcggtgtgg acagcacctt tgcagcctat tttgtgaaaa aaaacggcct
     2461 gaatccgctg gcagtacatc tcgacaacgg ctggaattca agaatcgcgg tcactaacat
     2521 tgaaaaaacc cttaaaacgc tgaatattga tttgtacacc catgtgattg actgggaaga
     2581 gtttaaagac ttacaattgt cctttctgaa agcctccacg ccggattcag aggtgccttc
     2641 ggatcatgcc attactgcac tgctctacaa ggtcgctgcc caacacggcg taaaatacat
     2701 acttgccggt accaatatca cctctgaatc cattttaccc aaagcatggt cgcaagggca
     2761 cagcgactgg aaatacatcc attctcttca aaaacaattc ggcacagccc ggctgaaaac
     2821 ctttccgcac tatggcatcc tgcggttgtt ttactatatt gttatccgca aaataaagta
     2881 tatcagcatt ttgaattata cggattatgc caaagaagaa gccaaaaaac ttcttatcag
     2941 cgagctgggc tgggaagatt atggaggcaa gcaccatgag tcggtttata cccattttta
     3001 ccaatcatac ctcttgccca aaaagtttgg tttcgataag cgcagggcac acctgtcgag
     3061 cctcatcatg gccggccaga ttacccgcga agaagccctt gaagaaatga aaaaacccgc
     3121 catcagcgac gatatggccc gtacagaaga agaatatatc atcaacaaat tcgggcttac
     3181 ccgcgaatct tacgaccgta ttatgaatac cccgcccaaa agctttcatg actacccctc
     3241 ctacttcaac tcgttttatt ataaattttt cttcaaaatt tataaagctc tcggagcgct
     3301 gggcgtgaaa aaatcggata tttaaaatat tgccgggcgt ttattctttc tcaaaaagaa
     3361 caaaaatcca atagggcact ttgggtgaaa taatcatatt gaatgcgtcg aaaaggaagg
     3421 caagcgggtg agatttataa ttatgcacaa actcatgctc gcaaaacggg cacatggttt
     3481 tttcgtcttt aatttttttt gcaaaataag gaaaccagga agccggcggg caaagtctta
     3541 tctttagttt tgccaggatt ttattgtacc gtcgcacagg tgtgccataa taaaatgtct
     3601 tgctgatttt gcatcctgag aataactccc cgagtttttc ccgcgtaaaa ctgtgcaggt
     3661 gataggaatg attataattt tttccgcact gcggacagtg aagtttgaat ttatctaggt
     3721 tttcgttgtt gggtacagta actaaaatat attttttaga cagcctggtt atttccccga
     3781 tagttttttt cagggtttct tcgtcgaggt gttccagcat ttcgctcgaa agcaccatat
     3841 cgaaagaggc atcaggcaag ggaatactat ctgaggaggc ctgaagtttt tcggttttta
     3901 catgctcaag cgcgtgggca ctgcgatcca ctcccagcat ggcatatttt tttccaagtt
     3961 cgttagtgat aacaccattg ccacagccaa tgtcaatgat tgaaactacc tctggcggta
     4021 tggtttccag gattttttgt atttttcccg caagcccggc ttcgtcaatc acatcccact
     4081 gatactgttc gtaatatttt ttattgtcga tcatccgaaa actttttttc atagcatttt
     4141 ttatcaccag tttactgata aaccttccct gctccggtgt taccgctttt ttctctttac
     4201 caaaaaacat cataacgtaa gtgccataag ccacacaagg ccctatcaac atgggtttgt
     4261 atttaattct tttggaacat gaaattccgg cggctatagc gttgaatccc ataaaatagc
     4321 atccgcggcc ggtcattttg tgcgctttca ggtgtcctgt ctttcggtcg gtaggccggt
     4381 gatgaagcac ttcaagggat tggtcggcga ggatggtgta ctggtaaaat ttagcgagca
     4441 tttcatccac ggcatcccag ccgtcaattt cgcgcagccc gcctatatgg tcgagaaaag
     4501 ttttccggta agctttaatc gcgccacgca catgatcttt attggtaatc ttttcgcgta
     4561 tccattcgcc gtttatttca accagacaga tgccgccgca aacggccagc ttctggtcgg
     4621 cggcaaaatg cgcagccacc gcctcaaaat aattgggctt caggctaaga tcggcatcca
     4681 gttttacaac aaaatcaata tcgggaataa tatggttgcg gtaacaatta ttgaaaatgc
     4741 gggcaatatt ggttccggcc atatggcttt ttttggcctt gttctgtacg agctttatcc
     4801 atggaaaatc agcggcaaag gctgtgataa tggcagccgt ttggtcggtg gagccatcat
     4861 cgtaaattac ccattgcaca ggctttagtg tttgtcctgc caccgattgg agcaggtttt
     4921 ttatgctgtt ttcttcatta aaaaccggag tgataatggc gtatctcatg caaaattttt
     4981 ttacaaaagt acctataaat ttccatccgt tatcgtggct tatgagatat tctttatagt
     5041 taatgctgta aaaaacccac tgtgtaaaaa agccacaaca cgtgagtgaa atatttgatt
     5101 ttaatgacaa ttttctcccg agtgtaaaac tttactttaa atttgcgcaa aaaccggcgg
     5161 caaagtgggc ataataaaga ggcaaagcat aaaaagcacg atagtgatat actgcggcat
     5221 gatgctgggg tatgtaaacc tgttgcttct ctcaccgctt attttgtcgc cagaacaaat
     5281 aggtctcacg cgggtattgt acgacggaag ctatatgttc gccatgttgg cccaattggg
     5341 aatctggagt gtggccacca agttttacag ttatttcagg gataaaaaca aaaaagacaa
     5401 tggttttctt ttttacatgg tatcaatacc ggcggtagga tttctaatac tgatatcgtt
     5461 attgcttatt ttcaatgaac agtttacagg actttttata caaaaatcgc ccctcattgt
     5521 tgactactat tattatttta tcccgctgat atttatcgtg atgtattacc aggtttttga
     5581 gacctacagc agtttgcaat acaggatagt ggtccccaac cttctaaggg aagtactgaa
     5641 taagtttatg tatctgataa ttatcctgct gcttttgtcg caaatgatca attttaagat
     5701 gtttgtcaat ttttatgtgc tctcttacgg agtttcgctc atagtgctga tactgtacct
     5761 gcgctccatg cacacgcttc atttcaaacc cgatttcagt tttgtaagcc ccgaactgcg
     5821 gaaacaaatg attacgtatg gtgtctttgt atttttcgga agtctgggtg cttttgccgt
     5881 ggcccgtatg gatgttatca tgctcagttc cttatcaggc ttgtattttg ccggaattta
     5941 cagcatttcc tacctgttgg gaaatgtgat tgaagtaccc cgcaaagcca ttgcccagat
     6001 atccgttccc ataattgccg atgcatggaa aaataacgat attgacaaaa tagacgagat
     6061 ctacaaaaaa acttcgataa atcaagctat cgtagctggc ctgatatttt tggtaatctg
     6121 gataaacatc gaagccattt tttcgcttat ccccaacagt tcggtgtata ttgccggtaa
     6181 atatgtcgcc ctttttatcg ggctggccag atttattgat gctgcaaccg gtgtcaacac
     6241 agaaatattg atgaattcgc ataaacacta caaatttaac ctggtattat tgtttgccat
     6301 gggtcttctc accattgtcc ttaacctgat actgattcct atcctcaata tgtacggcgc
     6361 gtgtctttcc gttttgattt ctattttgtt gtataatatt ttcaaatttg tttttatcta
     6421 caaaaaatgg aaaatacagc catttacctt caacacatta aaagtgattc tgctccttat
     6481 tgtcctggtt ttcgtatctt atctattccc aaaactccac aacccgatat tgcagattat
     6541 tatggtttcg gttcctgtca taactgttta cggtctggcc tgctattgga tgaaagtttc
     6601 ggccgatgcc aatgaccttg caaacctggc gataaataaa atcaaacggc tgctgtcgcc
     6661 caaaaacaaa taaccattcc atgatattta gaaattatta tgattgtttt actaaattat
     6721 attccatgaa caaattgtta cttttacaga aaacgttcag acactatgcc tgtgtggcat
     6781 taatgacagc actaagtgct acttctgcga tagcccagac agacattctt tttaaagaag
     6841 attttgaaaa ttatagttcg gaaggcgctt ttggcgctgt aacagccgta aacggctggc
     6901 ttcaaaactc aacagaccct aataatgcca tccgtaatct ctggtgctca tacagcggca
     6961 ccaattattg cccttcctgc accaatccca ccgccggagt gcgcaccatc agcggcgtac
     7021 gttccgcaca aatctgttat atcaatgctt ccaacaccat tgcctgggat ggaaattact
     7081 acggggtagg aacctataac accgccacca ccacaacaga ccggcatatt tttcattcga
     7141 taaactctac aggctatcaa aacattgttc tttctttcaa ttggaaatgt gccggcgaaa
     7201 tctacggcgg gaaccctgta gattacgggc aggctgccta ccgtccagga aacacaggca
     7261 gttggataaa gctgacatca gggggataca acaacacagg attgctttgc aatacaggcc
     7321 agatggtaaa tgccgcctct tacaatcttc cggcagcatg caataacact acttttcaaa
     7381 ttgctttttt ctggaataat gataatattg gagggagcgg gcctgcattt attgttgatg
     7441 acatcctggt taccggaaca agaaccacac cggattactg ccggccctat catcagtacg
     7501 gaaattgctc tccatggaac gactgccagt atgtaggaat aaccaacgtt tcgtgcggaa
     7561 gcatcaacaa tacaacaagc attgcaaatt ttccaccggc ctattcggat tttaccaata
     7621 taagcaccaa tttgattgtc aataatacct ataatgtcgg ggtaacatac tccgacaacg
     7681 gtagtcctgt gaattatggg aagatagcac tttggataga tttcaacggc gacctggatt
     7741 ttttggatgc aaatgaattt attggcgaac aacaggccac ggcaacaggc caggtcaaaa
     7801 acttcggctt tactgttcct tccgatgctt ttaacggcag tgtggttatg cgtgtgcgct
     7861 gtgcctatac tgatgaggga ttcgccgcta ccgatccgtg ttctttacgc gattatggag
     7921 aaacagaaga ttatatgctg aatattacca acatcagtac ggtaatcgac ctgcctgaaa
     7981 atgaccagtt tactgctatg cccaatcctg caacggatga aatttatgtg aaaaacaatg
     8041 atctttccga tcccataacc ctgataatca ttaccgatat taccggcaaa cagcttatac
     8101 aaaaagatgg tctcgcctgt cacgacgaaa gaatttcact gaaacagcta aacccgggaa
     8161 tatatttcct tgttattaaa acacataatg gacatcattc tgtaataaag attgcagttg
     8221 ccagatagca ttttgtgcgg attatctttg ataaacgggg aaccaaaggc ttggacgcaa
     8281 tgactgactg attcaaaatg ccttttatta tctaaaaatc tctttcagcg atgccttatc
     8341 gggataggta atcagcacag ccctgtgagg cccgtaaaag ataaaaaagc tgcctgcagc
     8401 cacagcggaa gccccgcttt tgacagcgtc ggcaaaatgc ctgatatggc cggccccgcc
     8461 cgcagccacc accggaacag taaccgcctg agttactttt tttatcagtt cagtatcgta
     8521 gcccgaaaaa gtgccatcgc ggtccaccga attaaggaat atctccccgg cgcctttgct
     8581 ttccatcagt tgagcatatt caacagggtc gagggaggtg gttttttgca gcgcataatc
     8641 aaaaacacgt agcctgccca ataaatcttt tttaatatct atggatacaa ccacgctcga
     8701 ggaacctgct acttctgagg cctgcgtaat aaaatcggga ttttttcgcg catatgcatt
     8761 aatagcaact ttttccacgc ccaggcgcgt aagctttttt acctgttcca ccgttgtgat
     8821 gcctcctcca tatccaaaag gcataaatgc ctcgcaactg atacgtgcaa tcaggtcata
     8881 attcggttcc ctgccttttt tggaagcttc aatatcaata aacaacaact cgtcaacttc
     8941 cttgtcattg aaaattttta ctgcattgat aggatcgcct acgtaagtag gatttttgaa
     9001 cttaaccgac ttcaccagcc cgtcgttttt cagcaacagg cagggtatca cgcgaatctt
     9061 aagcataagg ggcctccaca aagtttttca tgagcagcag gccgaattta tggctttttt
     9121 ccggatgaaa ttgcgtggcg aatatattac tgcgctgcag gcttgaggta aactcaacgc
     9181 catacattgt ggtagcagca atatcttcgg ggtggcggca ctccacatga tatgaatgga
     9241 caaaataaaa ggacgcctcg tcgtacatat tccgaaagat gccctctttg ttttttacca
     9301 cgacgtcgtt ccagcccatg tgcggaatgc gcaacaaccg gttattgtca tcaaaacgga
     9361 attttttaca ttcggcatcg atccatccca ggccgggcaa aacgccttct tcgctgcttc
     9421 gggccatcaa ctgcatgccc aggcatatac ccagcacggg aacctgttct tccaatacct
     9481 ttttattcaa cacatcaata agtccttttt ccctgaggtt tttcatgccg ttatcaaaag
     9541 cgcccacacc gggaataata atttttcccg caccggctac aagttcaggg tcggaagtta
     9601 ccaaactctc cccgcctgcc ttttttatca tattgacgat ggagccaata tttcccatcc
     9661 cgtaatctac aacgactatc atgaaatgct gctgaggtgt aatgattatt acttttttcg
     9721 ttttggtacc gaatttttct tttctttggc tgcgacagaa gcaacaggca ctt
//
LOCUS       DAOSFP010000033         9695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5977_length_9695_cov_3.579979, whole genome shotgun sequence.
ACCESSION   DAOSFP010000033 DAOSFP010000000
VERSION     DAOSFP010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5977_length_9695_cov_3.579979"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="PLI16_01815"
     CDS             complement(<1..514)
                     /locus_tag="PLI16_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase"
                     /protein_id="HOH83326.1"
                     /translation="MREHLQHKVFTIISDIAQAQQLEVFAIGGFVRDSILKRPCKDID
                     IVVKGSGISLAEQVAAAIGPGCQVNIFKNFGTAMLHFEDYDIEFVGARKESYHADSRK
                     PAVEEGTLEDDQNRRDFTINALFYNPLTEKVLDFTGQGISDMEHGIVRGIGNIALRFE
                     EDPVRLLRALK"
     gene            complement(555..1118)
                     /locus_tag="PLI16_01820"
     CDS             complement(555..1118)
                     /locus_tag="PLI16_01820"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOH83327.1"
                     /translation="MKEELLKTFEIVRKGGVILYPTDTVWGLGCDATVSKSIDRIAQI
                     KKRPVNKAMIVLLDLQEKLYQYVEDIPEIAFDFMKNIITPLTIIYPRAKNLPKNLIAE
                     DGSIAIRITHDEFCNKLINMMNAPLVSTSANFSGEPTPLIYSKINPGIIDQVDYVVDL
                     NKTRIKEVKSSTVIKFTDDGEYKILRS"
     gene            complement(1115..2065)
                     /locus_tag="PLI16_01825"
     CDS             complement(1115..2065)
                     /locus_tag="PLI16_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOH83328.1"
                     /translation="MILATPLIESFAAQQPPVEIDFLLKKGCETLFEGHPYLHEVLIW
                     DKNKKKYAHLYHLLKQIRKRKYDHVINLQRFASTGLLTVLSKAKITCGFDKNPFSFLY
                     KNKVRHIIGSGTRLHEIDRNLMLIENIVAPKRQIKLYPSPADFEKVSPYKSMPYICIA
                     PASLWETKRFPVIKWVEFLNSLPSGIPVMLLGSKNDNDLCNNIIKKSVHKPIENLAGK
                     LSFLQTAALLKDAHMSFTNDSAPQHLASAVNAPVAAIFCSTVPAFGFGPLSGRSLIIE
                     THENLACRPCGIHGYTACPEKHYQCAVTINNQQLLDCLAL"
     gene            2234..4591
                     /locus_tag="PLI16_01830"
     CDS             2234..4591
                     /locus_tag="PLI16_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83329.1"
                     /translation="MKKQYFGFILYVIFLFSCGRLSGQVVSLNRIGQTPGSAGFHVNW
                     DSTAKRLIVGCGTSIWIYDMGDPQNYSIIAKRPFLGLINETEVYGDVLFAAATHDGVY
                     ALDYNSHELDVLAHYDMQGMGDTAAYDMWRTNDTLYIADNFRVRMLKYIPGTGFVKLG
                     SFGPPNAFCVARRGDYIAVGGQGVLYLTPDTAVRGNIHIYHKNNLNTPVAVWQSELVS
                     VVQDIQFADRNDSIIYVCAGPEEILFYKSNFFALNFATDTLFPADTFSLSGGILGFAQ
                     LNIMNMDSQNDTLFLVTTAATNTSVFPAAYMPIIDATGLPADSMRKIGYVIPGLWHFD
                     AALMDGTPYIAMSSEWCGVLVSNISQLQPADTLGFLETGGWCVNNHLQNGSLWACHEG
                     YGLVAYNTDSLKYVNGFDTRAQLMHIYDVNNHFFCSDIAFLNDSLMMVNSSEVYNIKP
                     WLSGGTQQLAWDMNKNYMTHMNVLQTNARIRMVASYDNLLGKQWITLFDPFDSGGSFH
                     DLALDSMNSDSRGIAVSGDTVYYGKTINNIHYLCAQRVYNDGFMFLDTIKLSLGFFGL
                     FDDEIHSISIENGIIAVAYGAQFAWFNWVGGTLNELAMDFKFGQRAMGLELKNKYLYV
                     ADRFFGLKVYDISSMNSAVLVAECRGTGGWKNLYGSGTVTVGPDGTIYLTDFHAGVIM
                     IEPFDHTLGTFSEKGISTDDKMIVYPNPAEGMLNIDFVANEKGRADIEIYDLYGKKIS
                     ASAIMNCNRGKNHKTINLKGLPAATYVLVVRQLATTRSAVFGICK"
     gene            4766..5239
                     /locus_tag="PLI16_01835"
     CDS             4766..5239
                     /locus_tag="PLI16_01835"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOH83330.1"
                     /translation="MKSTKVLSALLTAMVVFMLTSCCDNNKCKKQETLVSPDSLIGKF
                     ATAWNQKDTNAVADIFSDNALFLYRNYSLKGKDSIMIKWVNPTIFNMANLKLTNIASG
                     ASADMAFITGFYTFDLTQNDSVVASTDGNYTSVWKKQSDSLWTMELMHQGDLRPE"
     gene            5556..7154
                     /locus_tag="PLI16_01840"
     CDS             5556..7154
                     /locus_tag="PLI16_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH83331.1"
                     /translation="MIRIITSLALLFYFFAPAFTQTQEQKKLNNWYNKDLQMDKVFGI
                     STEKAYKELLKNKKSQTVIVAVIDGGTDIMHEDLKENIWVNADEIPGNGIDDDKNGYI
                     DDIHGWNFIGNAKGENVDKDNLEVTRLYKELDPKYRGKDKSSVADKEEFELYQKVKEV
                     YNEKSQESQANIGMAQSGKELKVADSIVKAYLKKPDYSLKDLKKLNKAPAAVAESAEK
                     LRVWQLFGISADDLIAYGDEINSELTYHYNLDFDGREIVGDDYKDNSNRFYGNNDVTG
                     PEPAHGTFVAGEIGAVRNNGKGVDGIASDVKLMIIRVVPNGDERDKDVANGIRYAIEN
                     GAKVINMSFGKAFSPQKKFVDEVLPLADKYDVLLVHAAGNDAENNDLVANYPTHIGAD
                     GKPITNNWLTVGASSMKVDDDLAANFSNYGTKTVDLFAPGVDLWGTKPGNRYGAFSGT
                     SMASPVVAGMAAVIRSYYPQLSASEVKEAIMKSVTSVDQMVQKPTEAGKGERVSFSTL
                     SVSGGVANLYNALLIAEQIAAAKK"
     gene            complement(7176..8114)
                     /gene="trxB"
                     /locus_tag="PLI16_01845"
     CDS             complement(7176..8114)
                     /gene="trxB"
                     /locus_tag="PLI16_01845"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOH83332.1"
                     /translation="MTEEIEKVRVLIIGSGPAGYTAAIYTSRAELKPVLYQGLQPGGQ
                     LTITTDVENFPGYPEGISGYQMMEDMRKQAERFGADIRWGVVTSVDFSERPFKVVADE
                     TKTILADSVIIATGASARWLGLETEEKYRGYGVSACATCDGFFYKGKDVAVIGGGDTA
                     AEEATYLAKICKKVYLIHRRTEMRASKAMQNKVFNTPNIERLFGYVPVEITGEKNVDG
                     LTIKNVTTQEISNIVVDGVFVAIGHTPNSAIFKGQIEMDENGYIITVPGSTKTNVEGV
                     FAAGDVQDTHYKQAATAAGTGCMAAIEAERFLNSVS"
     gene            complement(8126..9007)
                     /locus_tag="PLI16_01850"
     CDS             complement(8126..9007)
                     /locus_tag="PLI16_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HOH83333.1"
                     /translation="MKIKSFIVPVILLISAFGFFSCEKEIEIDLPEEPPKIVIEGYIE
                     NGMPARVSITRSAPYFDPIDSLTLINSIVANALVIVSDGITSDTLQQDTILALPPIVY
                     VGSTVIGEIGKTYYLTVQVNGQTFTSQTTLPAPKAFDSLWFTLMPDNDSIGDVFAVGT
                     DNGAQTNYYRVFTKNTSIDFTFVPIFESVWDDKFFNGMTFTIQLYHGMVSNMMDMFQD
                     DGGRGLGYKVGDQVIVKLCTMDYYSYKFWAAAESEITFGANPFMTTNSVPTNIEGGAI
                     GCWTGYTATYDTIICNP"
     gene            complement(9012..>9695)
                     /locus_tag="PLI16_01855"
     CDS             complement(9012..>9695)
                     /locus_tag="PLI16_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83334.1"
                     /translation="FRNFADNMWETSFEVYYKDMRNQIEFKEGFSAVDNLKNNTDNNF
                     TFGKGWSYGGELFIKKRQGKTTGWIGYTLSWTKRKFPEINGGETYPAKYDRRHDISIV
                     ITQQLGKRWSISGVWVYSIGNAMTLPLSRYFLSGSIVNEYSKRNAFRMPAYHRLDISA
                     TFSSKDKPGRKFHWDLNFGVYNLYSRMNPYYIYFETTGDLTTMNLETKAKQVSLFPII
                     PSVTWNFNF"
BASE COUNT         2589 a         1962 c         2356 g         2788 t
ORIGIN      
        1 gtttcagcgc gcgcagcagc cggaccgggt cctcttcgaa gcgcagcgct atattgccga
       61 tgccgcggac aatgccatgt tccatatcgc tgatcccctg ccccgtgaag tcgaggactt
      121 tctcggtcag cggattgtaa aacagagcgt tgatggtgaa atcacgccgg ttctggtcgt
      181 cttcaagggt gccttcttct acggcgggct tgcgcgagtc ggcatggtac gactctttgc
      241 gtgctcccac aaattcgata tcatagtctt caaaatgcaa catggccgtg ccaaagtttt
      301 tgaagatatt tacctggcat ccggggccaa tggctgcagc tacctgttct gccaggctta
      361 tgccgctgcc tttcaccaca atgtcgatat ctttgcaggg acgtttgaga atgctgtcgc
      421 gtacaaaccc gcctatggca aaaacttcga gctgttgtgc ctgcgcaata tccgaaatga
      481 tagtgaaaac tttatgttgt aagtgttctc tcaaaattcc aatccgtgag gggcaaaatt
      541 ataaaaaaac cgcgttatga gcggagaatt ttgtattccc cgtcatcggt aaatttgatt
      601 acggtggagg atttcacttc tttgatgcgt gtcttgttaa ggtccaccac atagtccacc
      661 tggtcgatga tgcccggatt aatcttgctg tatatcagcg gtgtcggttc gcccgaaaag
      721 ttggctgaag tggagaccag cggcgcgttc atcatgttga tcagtttgtt gcaaaactca
      781 tcatgggtga tacgtatggc tatgctgcca tcttcggcaa tcaggttttt cggaaggttt
      841 ttggccctgg gatatataat ggttagcgga gtaatgatgt tcttcataaa atcaaaagca
      901 atttcaggaa tgtcctcaac atactgataa agtttttcct gcaagtcaag aaggacgatc
      961 atggctttgt ttacgggacg tttttttatc tgggcaatcc ggtctatcga tttgcttaca
     1021 gtggcatcac atcccagtcc ccacacggta tcagtggggt agaggatcac tccgcctttg
     1081 cggactattt caaaagtctt taacagctct tctttcatag ggctaaacaa tctaatagtt
     1141 gttggttgtt gatggtaaca gcacattggt aatgcttctc ggggcaagct gtgtagccgt
     1201 gaataccaca aggccggcac gcgaggtttt cgtgggtttc gataataagg gaacgtccgg
     1261 ataagggccc aaagccaaag gcaggcacgg tggagcagaa aattgctgcc acaggggcat
     1321 tcactgccga agcaaggtgt tgtggtgccg aatcgttggt aaaactcata tgtgcatctt
     1381 ttaacaaagc ggcagtctga agaaaactca gtttgccggc aaggttttcg atgggtttgt
     1441 gtacgctttt tttaatgata ttgttacata aatcattatc gttttttgat cccaaaagca
     1501 taactggtat tccggagggc agcgagttga gaaattccac ccattttatt accggaaacc
     1561 ttttggtttc ccacaacgag gccggagcaa tacagatata aggcatggat ttatatgggc
     1621 tcactttttc gaaatcggcc ggtgagggat atagttttat ctgccttttg ggtgcaacaa
     1681 tattttcgat gagcataagg ttgcggtcaa tttcatgcag ccgtgtgccg ctgccgatga
     1741 tgtgccgtac cttatttttg tacaaaaatg aaaaaggatt tttatcgaag ccgcaggtga
     1801 tctttgcttt tgacaatacg gtcaacaggc ctgtagaagc aaaacgttgc aggttaatca
     1861 catggtcgta ttttcttttt ctgatttgtt tcagcagatg ataaagatgt gcatattttt
     1921 ttttgttttt atcccatatt aaaacctcgt gaaggtatgg atggccttca aaaagcgttt
     1981 cgcatccctt tttcagcaga aaatcaatct caaccggagg ctgctgtgcg gcaaaactct
     2041 ctatcagcgg tgtggccagg atcacatcgc caatggaggc agtttgtatg atgaggatac
     2101 gtttcagggt ggcaaatttt atgatgctaa attataattt tttttttaat tctgcacagt
     2161 tttggcgggt tcctattaag ttttatatcc ggaaaaattt cttacatttg gaatataaat
     2221 tattgaaaaa cacatgaaaa aacaatactt tggtttcatt ctatatgtga tttttttgtt
     2281 ttcctgcggc cggttgtcag ggcaggttgt ctcgctcaac cgcataggtc aaacgccggg
     2341 tagtgccggg ttccatgtga actgggacag cacggccaaa agactgattg ttggctgtgg
     2401 caccagcatc tggatatatg atatgggcga tccgcagaat tattccatta ttgcaaaacg
     2461 tccattcctt ggtctgataa acgaaaccga ggtatacggc gatgtgcttt ttgctgctgc
     2521 cacccacgac ggcgtgtatg cactcgatta taattcgcat gagctggatg tgctggcaca
     2581 ttatgatatg cagggcatgg gcgacacagc agcttatgat atgtggcgaa cgaatgatac
     2641 gctgtatatt gccgataatt tcagggtgcg catgctgaaa tatataccgg ggacgggttt
     2701 tgtaaaactg ggcagtttcg gtccgccgaa tgcattttgc gtggcgcgca gaggcgatta
     2761 cattgcggta ggagggcagg gcgttttata tttgactcct gatacagcgg tgcgcggaaa
     2821 catacatatt tatcacaaaa acaacctgaa caccccggta gctgtatggc agagtgaact
     2881 ggtcagcgtg gtgcaggata tacagtttgc cgatcgtaac gacagtatca tctatgtgtg
     2941 tgcagggcca gaggaaatac tgttttacaa gtcaaatttt tttgcattga attttgccac
     3001 ggataccttg tttccggccg atactttttc gctgagcggc ggcatactgg gttttgcaca
     3061 actgaatatc atgaacatgg attcgcagaa cgatactttg tttttggtta ctactgcagc
     3121 cacgaatacc tccgtatttc ctgcggcata tatgccaata attgatgcga ccggcttgcc
     3181 cgcggatagc atgcgaaaga ttggttatgt cattccgggc ctttggcatt tcgacgcagc
     3241 acttatggac ggaacgcctt atattgccat gtcgtcggag tggtgcggag tactggtgag
     3301 caatatctcg cagttgcaac ccgccgatac gctgggattt ctggaaaccg gcggatggtg
     3361 tgttaacaat cacctgcaaa acggttcttt atgggcatgt cacgaaggct atgggttggt
     3421 ggcatataat acggacagtc tgaaatatgt caacggattt gatacccgcg cccagcttat
     3481 gcacatttac gatgtgaaca accatttttt ctgcagcgat atagcatttc tgaacgattc
     3541 cctgatgatg gtgaattcat cggaagttta caatataaaa ccctggttgt cgggcgggac
     3601 acagcaactg gcctgggata tgaacaaaaa ctatatgaca cacatgaacg tgttgcagac
     3661 gaatgcgcgc atacgaatgg tggcttccta cgataacctc ctgggtaaac aatggataac
     3721 tctttttgat ccttttgatt ctggaggcag ttttcacgat ctggctctgg attcgatgaa
     3781 cagcgattcg agaggcatag ccgtttcggg agatacggtt tactatggaa aaaccattaa
     3841 taacatacat tacctgtgtg cacagcgtgt atataatgac ggatttatgt ttctggatac
     3901 cataaaactt tcgcttggtt tttttggtct tttcgatgat gagatacatt ccatatccat
     3961 tgaaaatgga atcattgccg tggcttatgg ggcacagttt gcgtggttta actgggtcgg
     4021 aggcaccctg aatgaacttg ccatggattt taagttcggc cagcgggcga tgggactgga
     4081 actgaaaaac aaatacctct atgttgcgga cagatttttc ggattaaaag tttatgatat
     4141 ctcttccatg aacagcgctg tgcttgtggc tgaatgccgc ggcacaggcg gatggaaaaa
     4201 cctctatgga agcggtaccg taactgtcgg gccggacggg actatttatt tgacggattt
     4261 tcacgcgggc gtaattatga ttgagccttt tgatcatacc ttaggaacat tcagtgaaaa
     4321 aggaatcagc acggatgata aaatgattgt ttaccccaat cctgcggaag gtatgctgaa
     4381 tattgacttt gtcgccaatg aaaagggcag ggccgatatt gaaatttatg atttatatgg
     4441 gaaaaagata agtgcttctg caattatgaa ttgcaacagg gggaagaatc acaaaactat
     4501 aaatttaaag ggtttgcctg ctgcgacata tgtgctggtt gtgagacagc ttgcaacaac
     4561 gcgatcagca gtgttcggca tatgcaaata gacaatttct tttgattttc ttgctaagca
     4621 tctattgtat atggaaaagt tcatttttgc atgtgcatta cacataaaat ttattgtgaa
     4681 tacaatatta aattataaaa aattaactaa acggaatgtt tttgattata tttgctgtaa
     4741 ttatttttta taaatccaaa aaattatgaa atcaacaaaa gtattaagtg ctctactcac
     4801 agcgatggtt gtttttatgc taacatcatg ttgtgataat aacaaatgca aaaagcagga
     4861 aacgctggtg tctccggatt cgctgattgg aaagttcgct accgcatgga atcaaaaaga
     4921 taccaatgct gttgctgata tcttttccga caatgctctg tttttataca gaaactattc
     4981 tttaaaagga aaggattcca tcatgattaa atgggttaat ccaaccattt ttaatatggc
     5041 caacctgaag ctgaccaata ttgcctccgg agcatctgct gatatggctt ttatcacggg
     5101 tttttatact tttgatttaa ctcagaatga ttcggtggtg gcttcaacgg atggtaatta
     5161 tacctccgta tggaaaaagc agtccgacag tctgtggacc atggaattaa tgcatcaggg
     5221 tgacctgcgt cccgaataaa acagaaggca taactatagg tgtttcttta ttgagctgcc
     5281 ttgtatcgcg aggtttgaag gttataaatt tgttgttact gatcttctaa aaaaaaagga
     5341 taaaaaacct tttctggccg gatcttctaa aggttgggta taatcgcaat cgaacatatt
     5401 gccataatcc tgttattgtc agcactggaa taaataattc gctgagttta gcgaatcaaa
     5461 agttttttcg tgtgtttaag aattttattc ttttaaagtc ccgcttatat actaatattg
     5521 caggaatttt gttataataa aaaaatctaa aagtcatgat aagaataata acctcactgg
     5581 cattactatt ttattttttt gcacctgctt ttactcaaac acaggaacag aagaaactca
     5641 acaactggta caataaagac ttgcaaatgg ataaggtttt tggtatcagt accgaaaagg
     5701 cttacaaaga actgttgaag aataaaaaat cacaaaccgt tattgttgcc gtaattgatg
     5761 gaggaacgga tattatgcat gaagacctga aagaaaatat ttgggtcaac gcggacgaaa
     5821 ttcccggcaa cggtattgat gatgataaaa acggctatat tgacgatatc cacggctgga
     5881 attttatcgg gaatgcaaaa ggcgaaaatg tggataagga taacctggag gtgacacgcc
     5941 tatataagga actcgacccg aaatacaggg gaaaggataa gtcatccgtg gccgataaag
     6001 aggaatttga gctgtaccaa aaggtaaaag aggtttataa tgaaaaatct caggaatcgc
     6061 aggctaatat tggcatggca caatcgggca aggaactcaa agttgccgac agtattgtga
     6121 aagcatacct gaaaaagccg gattactctt tgaaagacct gaaaaaacta aataaggcgc
     6181 cggcggctgt ggctgaaagc gccgaaaaac tcagggtatg gcagttgttc ggtatcagtg
     6241 ccgatgatct gattgcatac ggagatgaaa taaatagcga gctcacctat cattataatc
     6301 ttgattttga tggacgagaa attgttggtg atgattataa ggataacagc aaccgttttt
     6361 atggcaacaa cgatgttaca ggccccgagc ctgcacacgg cacttttgtt gccggcgaga
     6421 tcggcgctgt gcggaacaac ggcaaaggtg ttgacggtat agcgtccgat gttaaactta
     6481 tgattataag ggttgttccc aatggcgatg agcgcgacaa ggatgtggca aacggtattc
     6541 gctatgctat tgaaaacggc gccaaagtca tcaatatgag cttcgggaaa gctttttcgc
     6601 cccagaagaa atttgtggac gaggtgttgc cactggctga taaatacgat gtgttgctgg
     6661 ttcatgcggc aggcaacgat gcagaaaaca acgacttggt tgctaattat ccgacacaca
     6721 tcggggccga cgggaagccc atcaccaaca actggcttac tgtaggcgca agttctatga
     6781 aggtggatga cgacctggct gccaactttt ccaattatgg caccaaaacg gttgacttat
     6841 ttgcccccgg tgttgatctg tggggtacta agcctggtaa ccgctatgga gcattcagcg
     6901 ggacaagtat ggccagtcct gtggtagccg gcatggctgc cgtcatcaga agttattatc
     6961 ctcaattgtc ggcttcagaa gtgaaagaag ccattatgaa atcggtaacc agtgtggatc
     7021 agatggtgca gaagccaaca gaggcgggca aaggcgaaag agtaagtttc agcacactgt
     7081 cggttagcgg cggcgtggca aatttataca atgcgttgct catcgctgaa cagatagctg
     7141 ctgcgaaaaa gtaatttatt ttctaaaaca cgcttttagg atacggagtt gagaaaacgt
     7201 tcagcttcga tggctgccat acaacccgtg ccggctgctg tggcggcttg tttatagtgg
     7261 gtgtcctgca cgtcgccggc tgcaaacaca ccttccacat tggttttggt gcttccgggc
     7321 accgttataa tgtagccgtt ttcatccatt tctatttgtc ctttgaaaat cgcggagttc
     7381 ggcgtatggc caatagcgac aaaaacacca tcaacaacga tgttgctgat ttcctgtgtt
     7441 gttacatttt ttattgtcag gccgtcaaca tttttttcgc cggtgatttc tacgggaaca
     7501 tagccgaaga gtctttcgat gttgggcgta ttgaacactt tgttttgcat ggctttggaa
     7561 gctctcatct cggttcttct atggatgagg tacacttttt tgcagatttt ggccagatac
     7621 gtagcttctt cggcagcggt atcgccaccc ccaattactg ccacatcttt acctttgtaa
     7681 aaaaaaccat cgcaggtggc acaggccgat acgccgtaac cccggtattt ttcttcggtt
     7741 tccaggccga gccatcgggc agaagctccg gtggcaatga tgacactgtc ggcaagtatg
     7801 gtctttgttt cgtcggcaac cactttaaaa ggcctttctg aaaaatccac actggtaaca
     7861 actccccaac ggatgtcggc accaaagcgt tcggcttgtt tgcgcatatc ttccatcatc
     7921 tgatagccgc tgatgccttc agggtatccc gggaagtttt ccacatcggt tgttatggtc
     7981 aattggccgc ccggctgcaa tccctgataa agtaccggtt tcagttccgc tctggaagta
     8041 tagatggctg ccgtgtagcc cgccggccct gagcctatga taagcactct tactttttca
     8101 atttcttctg tcatattttt ttgttttatg gattgcaaat aatggtgtcg taagtggcgg
     8161 tgtatcctgt ccagcatccg atagctccgc cttctatgtt ggtagggaca ctgttggtgg
     8221 tcataaaggg attggcgcca aaagttatct ccgattcagc agccgcccag aatttatagc
     8281 tgtaatagtc catggtacat aactttacaa taacctggtc gccaacctta tagcccagtc
     8341 cgcggccgcc gtcgtcttga aacatatcca tcatattgct gaccatacca tgataaagct
     8401 gtatggtgaa ggtcatacca ttaaaaaatt tgtcatccca caccgattcg aagatgggaa
     8461 caaaggtaaa gtcaatactg gtatttttgg taaatacccg gtaataattt gtctgagccc
     8521 cgttgtcggt acctacggca aagacatcgc caatgctgtc gttatcgggc atcagggtga
     8581 accaaagcga atcaaaagct tttggtgcag gcagcgtggt ttgcgaggta aaagtttgtc
     8641 cgtttacctg aacggtaagg taatacgttt ttcctatttc accgattaca gtgctgccta
     8701 cataaacgat gggcggcagg gccaggatgg tgtcctgctg caaggtgtcc gaagtaatgc
     8761 catcgctaac aatcaccaaa gcattggcta caatggagtt gatcagcgtg agggagtcga
     8821 tagggtcgaa atacggtgcg cttctggtga tagaaaccct ggcaggcatt ccgttttcaa
     8881 tatagccttc gatgacaatt ttgggtggct cttcgggaag gtcgatctcg atttcttttt
     8941 cgcaggagaa aaaaccgaat gcgcttatca acaatattac cggtactata aaggatttta
     9001 ttttcattgt cttaaaaatt gaagttccat gtaacagagg gtattatcgg gaataaggat
     9061 acctgtttgg ccttggtctc gaggttcatg gtggtcaggt cgccggtggt ttcaaagtag
     9121 atgtaatatg gattcatgcg gctgtacagg ttatacacgc caaaattcag gtcccagtga
     9181 aattttcggc cgggtttgtc tttacttgag aaagtggcgg agatatcgag ccggtggtat
     9241 gcaggcatac ggaaggcgtt gcgtttgctg tattcgttga cgatgcttcc cgagaggaag
     9301 taacgcgaca ggggcaaggt catggcattg cctatggaat aaacccatac gcctgaaatt
     9361 gaccagcgtt tgccgagttg ttgggtaatt acaatggaga tatcgtggcg gcggtcgtat
     9421 ttggcgggat aggtctcgcc tccgtttatt tcaggaaact tacgtttggt ccacgacaag
     9481 gtgtaaccta tccatccagt ggtttttccc tgacgctttt tgataaaaag ctctccgccg
     9541 tatgaccagc cttttccaaa ggtaaagttg ttgtcggtat tgtttttcag gttgtcaacg
     9601 gcagaaaatc cttccttgaa ttcaatctgg ttgcgcatat ctttgtaata tacttcaaag
     9661 gaggtttccc acatattatc ggcaaaattt ctgaa
//
LOCUS       DAOSFP010000034         9676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_5998_length_9676_cov_3.252988, whole genome shotgun sequence.
ACCESSION   DAOSFP010000034 DAOSFP010000000
VERSION     DAOSFP010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5998_length_9676_cov_3.252988"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2698
                     /locus_tag="PLI16_01860"
     CDS             <1..2698
                     /locus_tag="PLI16_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83335.1"
                     /translation="PGILIKKSLLAVCLLFFFVFADAQNNLVFQQEEEVELKISYAAS
                     PGQNQQITNFFIQEIARSLPKKTEFTQYVFVCTKTDRITATGPASYSATFEILNAGCS
                     GDMMYKGFSVADVLMPTHCSFTFNVFTDKAIPVYKKNVTEAPLNLGYNLLDSYKFTDT
                     TAFKKFSTRVDSFAFHYSDKAINRFNSKIKLIDEYFGSETIIDQCMKKIREIDYNNTD
                     MIIVYDISLKEVEKTSEELYNKEFPGKLALVNHDPVGFIDKYNVLAEAIFQTRHLLSQ
                     KLANLDKLYYEKGIVQLQNGNNEKAKTYFNRSVLYNPDFVPSQLELVKIFYYSDSLNQ
                     AADKVSYILQHLNPTPDIHKQVLLYTDSVYNALLTRGKEFNRLQKYNEAIEILEKCVS
                     FCDSLPGYLCADSHVKNLASARFGIYQSYLSVAQKAIDNGKFELAEIYISDARNYQKI
                     HKSAIINDAEALSKLEKIVLAYVAKGDTLIARKHYENGLTYLEKAKNIAEKNGIQLPL
                     TYEKSMKNAQAGIYINMLNKCRRQLALKQTEAAEKTLNEAIAHHNKYTGLITPSKAVD
                     TLLAKIRQYQCDDMINNGMVALNAGNYFTALQHFDKAKKLQQTYALKRYLPLDSLLMV
                     SARPVIDNQLLLTEKLVVAGNPDSARTMAENAASGLMAFGLSGDTALMLKLRLLKKDI
                     FDLQCRQTRNKFETLMTRANMAVARQDYITADSLYAMAGMVTDSFPQCTIDISQAIQG
                     KKEYLLPCNYQKMLQKASKALFHGDYNSYFYNYSEAENFFKTMHLNGFGLIHPALTDQ
                     IAVSADTAFVFAAAEFMLQNNRPEDGLLCIKTLKKLHYPQQQTKNLQLQSGAMMARKD
                     FAVNKALQPNVIVVSYIGNDTWLAFFKSSYIKTWKTLKKNSG"
     gene            2721..3485
                     /locus_tag="PLI16_01865"
     CDS             2721..3485
                     /locus_tag="PLI16_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOH83336.1"
                     /translation="MFFTISKITAFLLNPLTWIVILFVAALFFKNARRKKRITIAGVV
                     CLIFFTNPAIIDNLLRTWEKPYQLMDKNVTYTAGIVLGGGMVTIDQKHDRLIFHENTD
                     RFLQGLHLYENGQIQKIILSGGAGNLVFKQFLEAALIKRYLVEIGVPAEDILIDSTSD
                     NTYQNAVNVAKILEEQGRGGSYLLITSSLHMKRAKACFAKQGIITTGYPTNKIVGPHR
                     TDVGYYLIPNAGALVRWEKFIHEVLGYVIYAIMGYI"
     gene            3617..4855
                     /locus_tag="PLI16_01870"
     CDS             3617..4855
                     /locus_tag="PLI16_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOH83337.1"
                     /translation="MAQTLFIVILVIVVVNYLFGLLLDLLDSTRWSNILPSELSGIYD
                     AEKYAKSQDYEKANMRFSLISGGFSFLLLMLMICLGGFAWLDNVVRSVTAHPVLISLL
                     FFGALGLASDLLTTPFSAYHTFVIEQKFGFNTTTVTTFILDKLKSWLMAAVLGGGILS
                     FIVWIYGVTGSWFWIIAWMAMTFFSVFMSLFYSNIIVPLFNKQTLLAPGTLRDAIEKF
                     AVRAGFKLKNIYIINGSKRSKKANAYFTGFGPKKRIVLYDTLLGDFTDDEIVAILAHE
                     IGHYKKKHVLFGLVSGIISSGVLFYILSLVIGSPEMAGALGAGQASFHMGILAFGLLY
                     SPVSLVLGIAGNYFSRRHEFAADGYAVAHADANAFAQGLKKLYVMSLSNLRPHPFTVL
                     ITYSHPPLLQRLAAIEKKQC"
     gene            5088..>9676
                     /locus_tag="PLI16_01875"
     CDS             5088..>9676
                     /locus_tag="PLI16_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83338.1"
                     /translation="MRKVLFFFTFFIIYASISIGQGNMLSISSVLPPEITICGSEQKV
                     EISIVNPSPFGLSNVVLQITMPVGLVYQAGTITGATELNISNLSQPQFSLTNIPSQGA
                     RNISFFIAANCDLMDYIAQGQIVSMVARVNYLTSNNINTYDQHTNYLYHVKQPNLSII
                     SITNQTYSGNIGDVFTRCITITNGGLGALSNFTFTHTHGNGIQVNNVSIGVWNSAGGV
                     ETVTMNGSNFLGIGNNNALFENAELITICETIEVINCVSVGSSYEVKWGCNGQICQNS
                     ISTANVVFPNLTPNLVITPTAGVNTCLGLGNANTPSLRIINNGMGDAYNVFLDIFHAT
                     STINYDAGIRSNIDPASFTVQVGINGTPVSITPVQTFAASAQYCLSANPKGRVYINIP
                     LINAHDTVYLKWRYYNCCSDAPGGTRVLSGWRYQGTYKSICNNNYVISNSWGYVYRDL
                     YGTLVNELSPSNITSGETKNFSFLFSSYGNSYPSTTGRHWKFVVNLPPCLSYESGSFQ
                     IQRYTGTQYWYPSTVTVSGNTLTAIFDNNPPWDLYQSIVKFNLIANCANPGCVEGGNN
                     LTITSFCIPDGSCLCEVLVSRLTITVGVICPVICEGLNNKAFDTYRSSYGAPDNDNNG
                     LADPLPATLNFNLIRRDRAMFGDTITTLSQGLIKTSVAHPAWNYCYRVDSVLNGNQLS
                     FVDGLLEIHQYSTGNVYTCNVNTSSITTAGSTRKFNFDISPVTLAALGLLPAGFTYND
                     NDSIAFRVRYRVSSNPGGTILDCFVKNKMFVSDVPNPASFAHKFGCNVFEGRFSIIGY
                     YYTNWGPDNFNVNTCNQVTISQNYYLSIGPCCQNYAGGNLFPFEYRNWAHAYRFRVIL
                     PHGYQFISARFNQVRTAGTNGGSTSAWVNLIPVNPNSDTLVFMVEPFYETFGGAIPLS
                     DDGFHGTLQVTLAPTCNVVSNVASNVGYYWDFGPSGYLAGSSTPNSSNVTTHDVITYQ
                     GPDIFVQSILPTVLAYDDEVNWEISLSNTTNINALNTWFSGDESTGVTILEVWDLDNN
                     VVVLPVGDIYQIGVLSANAVRHFRIKATYTSCFPSTMMLYAGWNCNAGYPATVASYPC
                     TPDQLQLSLEPQVPNLIANITSPLSTVDLCDTTGYVVEGINVQLGTAYNLKLKVVLPA
                     GVSIIPGSCKLSYPTASPYVAIPDPTWISGTQWQWDISALNALIGADGLPGILDTTLN
                     SVKISFRVITNCFYTSGSVIGFNFHGQSHCGNYTGQEISMSSQLAITGATEPYLTDIH
                     VNTSYVSPCAINSTRLKIRMKNNGPLATGATDSISLTFPEGIFYIPNSFLAVHNAPVN
                     PLPVTEMPNNQQIVTWKIPPNVQVGDSVVFEINYFCQANVVECGIYYIDAMSYSSRNL
                     LCQLSGQNCDILVSTGNAQIPIYIYKSYLSLSNASAWSVPSGANQETVYTSYTISNNG
                     ESIIANNTVFMSYFNDVDGNQILSAPDVFLGADSINVYIDTNSVNPFNSHITVPVGSS
                     CKLIFALDTNANGCICELDQIFANVQLVNAGQDTVV"
BASE COUNT         2668 a         2036 c         2062 g         2910 t
ORIGIN      
        1 gccgggaata ttgataaaaa aatcgctgct tgcagtgtgt ttgctgtttt tttttgtttt
       61 tgccgatgcg caaaacaatc ttgtatttca gcaggaagaa gaggttgaat taaagatttc
      121 ttatgcagct tcacccggtc aaaaccagca aattaccaat ttctttattc aggaaattgc
      181 ccggagttta ccgaaaaaaa ctgaattcac ccagtacgtt tttgtatgta ctaaaactga
      241 ccgtataact gccacgggcc cagcatctta ttcggccaca tttgaaatac tgaatgccgg
      301 ctgtagcggc gatatgatgt acaaagggtt ttcggttgcc gatgtgctga tgcctacaca
      361 ttgttccttt acctttaatg tctttacaga taaggccatt cctgtgtata aaaaaaatgt
      421 aactgaggcg ccactgaatc ttggttacaa tttgttggat tcctataaat tcaccgacac
      481 aacggccttt aagaaatttt cgacccgtgt agatagtttt gcttttcatt attcggataa
      541 ggcaatcaac cgctttaatt ccaaaataaa acttattgat gaatactttg gttccgaaac
      601 cattatcgat caatgcatga agaaaatcag ggagattgat tacaacaaca cggatatgat
      661 tattgtttat gatatcagtc ttaaagaggt tgaaaaaaca tccgaggagt tgtataacaa
      721 agagtttccc ggaaaactgg cgttggttaa tcatgatcct gttggtttta tcgacaagta
      781 taacgtgctt gcagaagcta tttttcagac caggcacctg ctcagtcaaa aacttgctaa
      841 ccttgataaa ttgtattatg agaaagggat tgtgcaattg caaaatggga acaacgaaaa
      901 agccaagact tattttaacc gctcggtttt gtacaatccc gattttgttc cctcacagct
      961 tgagctggta aaaatatttt attacagtga tagcctgaac caggcggctg acaaggtttc
     1021 ctatattttg cagcacttaa atccaacccc cgacatacat aaacaagtgt tgttatatac
     1081 cgattcggtt tataacgcct tattgacaag agggaaggaa ttcaacaggc ttcagaaata
     1141 caatgaagct attgaaatat tggaaaaatg tgtgtcattt tgcgacagcc tccccggtta
     1201 tttatgtgcc gatagtcatg taaaaaacct tgcatcagcc cgttttggta tctatcagtc
     1261 ctatttaagt gttgcccaaa aagcgattga caatggtaag tttgagttgg cagaaatcta
     1321 tatttccgat gcaaggaatt accagaaaat ccataaaagc gctattatca acgatgcgga
     1381 agccttgtcg aaactcgaaa agattgtgct tgcctacgtt gcgaaaggcg acacgctgat
     1441 agccaggaaa cattacgaaa atgggctgac ttacctcgaa aaagcaaaaa atattgctga
     1501 aaaaaacggt atccagcttc cgctgacgta tgaaaaaagc atgaaaaatg cacaagccgg
     1561 catctatatt aacatgctta ataaatgcag gcggcagctg gcactgaaac aaaccgaagc
     1621 ggcagaaaaa acattaaatg aagccattgc tcaccataac aaatatacag gattgataac
     1681 tccttccaaa gcggtggata cgttgcttgc aaaaatcagg caatatcagt gcgatgatat
     1741 gattaataac ggtatggtgg ctttgaatgc aggcaattat tttaccgcac ttcagcattt
     1801 tgacaaggcc aaaaaattac agcaaacata cgcgttaaag cgctatctac cattggattc
     1861 gctgctgatg gtttcggcac ggcctgtcat agataatcag ttattgctga cggaaaaact
     1921 tgtggtggca ggtaatcccg actcggcacg gactatggct gaaaatgcgg cttcgggcct
     1981 tatggctttc ggattgtcgg gagacaccgc cctgatgctt aaactcagac tgctgaaaaa
     2041 agatattttc gacctgcagt gccggcagac caggaataag tttgaaacac tgatgacgcg
     2101 cgccaatatg gctgttgcac gtcaggatta tatcaccgca gattctttgt atgcgatggc
     2161 aggtatggta actgacagtt ttccgcaatg taccattgat atcagtcagg ctatccaggg
     2221 aaaaaaggaa tatctactgc catgtaatta tcagaaaatg ttgcagaaag ccagcaaagc
     2281 gcttttccat ggcgactata attcctattt ttataattat tctgaagcag agaacttttt
     2341 taaaaccatg catctgaatg gtttcggcct tatacacccg gcactcaccg atcagatagc
     2401 ggtctctgct gacactgctt ttgtttttgc ggctgcagaa tttatgcttc aaaacaacag
     2461 gcctgaagat ggattgcttt gtatcaaaac attgaagaaa ctgcactatc cgcaacagca
     2521 aacaaaaaac ctgcaattac agtcgggcgc catgatggcc cgcaaggact ttgccgtgaa
     2581 caaggcgttg cagcccaatg tcatcgtggt gagctatatc ggaaacgata cctggctggc
     2641 attttttaaa tcttcctaca tcaaaacctg gaaaacatta aaaaagaatt caggttaatt
     2701 tttttcgtta atttgtaggt atgtttttta ccatatctaa aatcactgca ttcctgttga
     2761 atccgctgac ctggatagtt attctttttg tcgctgcgtt gtttttcaaa aatgcccggc
     2821 gaaaaaaaag aattaccata gccggcgtgg tttgtttaat tttttttacc aatcctgcca
     2881 ttatcgataa cctgttacgc acctgggaaa agccttacca gttgatggat aaaaatgtca
     2941 cttacacggc cggcatagtt ttggggggag gtatggtaac tatcgaccag aagcatgacc
     3001 ggcttatttt tcatgaaaat accgacaggt ttttgcaggg gctgcattta tacgaaaacg
     3061 gacaaataca aaaaattata ctctcgggcg gagccggaaa cctggttttt aaacagttcc
     3121 ttgaagcagc tcttattaag cgttacctgg ttgaaatagg tgttccggca gaggatatcc
     3181 tgatagattc cacctccgac aatacctatc aaaacgccgt taatgttgcc aaaatcttag
     3241 aagagcaggg gcggggcggg agttatttgc ttattacttc ttcgctacac atgaaacggg
     3301 caaaagcctg ctttgccaaa cagggcatta ttactaccgg ctaccctacc aataaaatag
     3361 tgggccctca ccgcacggat gtgggttatt acctcatacc caatgccggc gccctggtgc
     3421 gttgggaaaa gtttatccat gaagtgttgg gctatgtgat ttatgccatt atggggtata
     3481 tttgattcct tggctgtcac aatagtcttt gatttgtttt ttgggtgctg tagtatgccg
     3541 gcatatgcct attcgtggtg tatgtataat gtcagttaca cctataatga ttacctttgt
     3601 accctaactt ttttgtatgg cgcagacact ttttattgtc attttggtca ttgttgttgt
     3661 taactatctg tttggcttgt tgctcgattt gctcgacagt acccgttgga gcaatatcct
     3721 gccctcagaa ctttccggaa tttacgacgc ggaaaaatac gctaaatcgc aggactatga
     3781 aaaagcgaat atgcgttttt cattgattag cggtggcttt agctttttgc tgctcatgtt
     3841 gatgatttgt ctgggtggtt ttgcctggct cgataatgtg gttaggtcag ttactgctca
     3901 cccggtactg atttccctgt tgttttttgg agcactgggc ctggcttccg acttgctgac
     3961 tacacctttc agcgcttacc acacctttgt catcgaacaa aaatttgggt ttaatactac
     4021 aactgttaca acttttatcc tggacaaact gaagtcctgg ttgatggcgg cagttctggg
     4081 cggtggaatc ttgtctttca tcgtgtggat atatggcgta acaggatcat ggttttggat
     4141 aattgcctgg atggctatga cgtttttttc tgtttttatg agcttgtttt attccaatat
     4201 cattgtgccg ctgtttaata aacaaacact gcttgcaccg ggtacattgc gcgatgctat
     4261 tgagaaattt gctgtcagag caggattcaa attgaaaaac atttacatca taaatggctc
     4321 caaacgttca aaaaaggcca acgcttattt cacaggtttc ggaccgaaga agaggattgt
     4381 gctatacgat accctgcttg gagatttcac cgatgatgaa attgttgcta ttcttgcaca
     4441 tgagataggt cactacaaaa agaagcatgt gttgttcgga ttggtttccg gcattatcag
     4501 tagcggcgtt ttattttata ttttgtcgtt agttataggc agccccgaaa tggcaggcgc
     4561 cttgggagcc ggacaggcaa gttttcatat gggcatactg gcttttggtc ttttgtacag
     4621 ccctgtttcg ttggttttgg gcatagcagg caattatttt tcacgaaggc acgagttcgc
     4681 agccgatggt tatgctgtgg cgcatgctga tgccaatgcg tttgcacagg gattaaaaaa
     4741 actatacgtg atgagcctta gtaatttgcg gccgcatcct tttacggtat taattactta
     4801 ttcgcacccg cctttgcttc aaaggttggc agctattgaa aaaaagcagt gttaaatgtt
     4861 ttctgttatt aatagttgaa gtcaaatgag gttggattaa taggttgaaa catatgatct
     4921 gaaaaatctt cataatttca ggtttcggaa tgcaacattt tttttgtata taataaaaat
     4981 ctacaaagaa aaaacctgat ttttacgtta tattgtacag tcatgtattt gctaaaaaat
     5041 ttaagtttat atttgaatat tcaaataaaa gttaccaggt attatctatg agaaaagttt
     5101 tattcttttt tacatttttt ataatctatg cctcaatttc tattggccag ggaaatatgt
     5161 tgagtatttc atctgtacta ccgcctgaaa ttactatttg cgggtcggaa caaaaggttg
     5221 aaatcagtat tgtgaatcct tctccttttg ggctttccaa tgtggtgttg caaatcacca
     5281 tgcccgtggg attggtttat caggcaggta ctattacagg agctacagaa cttaatatct
     5341 ccaatttaag tcagccccag ttttcattaa caaatattcc ttcgcaaggc gcacggaata
     5401 tttccttttt tattgcggca aattgcgacc tgatggatta tatcgcccag gggcagatag
     5461 tgtcaatggt tgcacgggtt aattacctga caagcaataa tattaatact tacgatcaac
     5521 ataccaatta tctgtaccat gtaaaacaac ccaatttatc tattatctcg attaccaatc
     5581 aaacgtattc cggcaatatc ggtgatgtat tcacccggtg tattaccatc accaatggcg
     5641 gccttggcgc tttgagcaat tttactttta cgcacacaca cggcaatggc atacaggtaa
     5701 acaatgtttc cattggcgtg tggaattctg ccggtggtgt ggaaacagta actatgaatg
     5761 gaagcaattt tttggggata ggaaataata atgctctttt tgaaaatgcc gaattaatta
     5821 ccatctgcga aaccatcgag gttattaact gtgtgtcggt gggctccagc tatgaagtga
     5881 aatggggctg taacggacag atttgtcaaa actccatatc aactgctaat gtggtttttc
     5941 cgaatttaac ccctaacctg gtaataactc ccacagcagg tgtaaacaca tgcctgggac
     6001 tgggcaatgc aaataccccg tctctgagga ttatcaacaa tggcatgggt gatgcttaca
     6061 acgtgtttct ggatattttc catgcaacat caactataaa ttatgatgca ggaataaggt
     6121 ccaacatcga tccggccagt tttacggtgc aggtaggcat taacggcaca ccggtcagta
     6181 tcacgccggt gcagactttt gcagcttcag cgcaatattg tttgagcgca aacccaaagg
     6241 gaagagtata tatcaatatt cctttaatca atgcacacga cacggtatat ctcaaatggc
     6301 gatattataa ttgctgttcc gatgcccccg gcggtacccg tgttctgtcc ggctggaggt
     6361 atcagggtac ctacaaaagc atttgtaata acaattatgt gatttcaaat agctggggat
     6421 atgtatacag ggacctctat ggtactttag ttaacgaatt atcgccttca aatattacca
     6481 gcggagaaac caagaacttt agttttcttt tctcaagtta tggcaattcc tatcccagca
     6541 ccactgggcg acactggaaa tttgtggtca atttgccgcc ctgtctttca tatgaaagcg
     6601 gtagcttcca gattcagaga tataccggta cccaatactg gtatccttcc acagtaaccg
     6661 tttctggtaa taccctgacg gccatttttg acaataaccc tccctgggat ctttatcagt
     6721 ccattgtaaa gtttaacctg attgctaatt gtgccaatcc gggatgtgtt gaaggaggta
     6781 ataaccttac tatcacgtcg ttttgtattc ctgacggcag ttgcttatgc gaagtgcttg
     6841 tctcaaggct gactataacc gtgggggtta tttgccccgt gatttgtgaa ggattaaata
     6901 ataaggcttt tgacacttat cgaagcagtt atggcgctcc ggataacgac aacaacggac
     6961 tggccgatcc gttgcctgca acattgaact ttaatctgat aagaagagac cgtgccatgt
     7021 ttggcgatac cataactacc ctgtcacaag gacttataaa gaccagcgtg gctcatccgg
     7081 catggaatta ttgttacagg gtggactctg ttttgaacgg caatcagctg agttttgtgg
     7141 atggcttgct ggagatacac cagtactcca ccggaaatgt atatacctgt aatgtgaata
     7201 cttcttcgat aaccacagcc ggaagtacac gaaaattcaa cttcgatata agccccgtaa
     7261 ctttagccgc cctgggtctc ctgcctgccg gatttacata caacgataat gattccattg
     7321 ctttcagggt gcgttaccgg gtaagcagca accccggagg aaccatactt gattgttttg
     7381 taaaaaacaa aatgtttgtc agcgatgttc ccaaccctgc atcctttgca cacaagtttg
     7441 gctgtaatgt ttttgaagga cgtttctcaa taattggcta ttactatacc aactgggggc
     7501 ctgataattt taacgtgaac acatgtaatc aggtaactat cagccagaat tattacctga
     7561 gtatcggccc ctgttgtcaa aactatgcag ggggcaattt gtttcctttt gaatatcgta
     7621 actgggcgca tgcatacagg ttccgggtta ttctccctca cggataccag tttatttctg
     7681 cacggtttaa ccaggtacgc acggcaggta ctaatggcgg ttccacatcg gcctgggtaa
     7741 atctgatacc tgtgaatcca aactccgata ccctggtttt tatggtagaa cctttttacg
     7801 aaaccttcgg aggcgctatc cctctgagtg atgacggctt tcatggtacc ctgcaggtta
     7861 ccctggctcc aacgtgcaat gttgtttcca atgtcgcttc caatgtgggt tattattggg
     7921 attttggccc atcaggctat ctggcgggat ccagtacccc caacagctct aatgttacta
     7981 cacacgatgt cataacctat cagggaccgg atatctttgt acaatcgata ctcccgacag
     8041 tattggctta tgatgatgag gtgaattggg aaatatcctt atccaatacc actaacatca
     8101 atgcgttaaa cacatggttc tcaggcgatg aatcaaccgg tgttacaatt cttgaagtat
     8161 gggatctgga taataatgtg gtggtattac cggtagggga tatctatcag attggagtgt
     8221 tgtctgctaa tgcagtcagg catttcagga taaaagccac ctatacctcg tgtttcccaa
     8281 gtaccatgat gctttatgcc gggtggaact gcaatgcagg atatcctgct acggttgcat
     8341 catatccttg tactcccgat caactgcagc tttcgctcga acctcaggtg cccaatctga
     8401 tagcaaatat taccagcccc ctgtcaaccg tggacctttg cgatactacc ggctatgtag
     8461 ttgaaggcat caatgttcaa ctgggtactg cgtacaatct gaaactcaaa gtggttttgc
     8521 cggcaggtgt ttctattatt cccggctcgt gcaagctcag ttatccaacg gcttctccct
     8581 atgttgccat tccggatcct acgtggattt caggcactca atggcaatgg gatatttcag
     8641 cgctcaacgc gctgattggt gcggatggat tgcccggtat attagatacg actctcaatt
     8701 ccgttaaaat ttctttcagg gttatcacga actgctttta tacctcggga agcgtcatcg
     8761 ggtttaactt tcatggacaa agccactgcg gcaattatac cgggcaggaa atctccatgt
     8821 cctcccaact ggctatcacc ggagctaccg agccatatct taccgacatc catgtcaaca
     8881 cttcctatgt ttctccttgt gccatcaaca gtacccggct gaagatcaga atgaagaata
     8941 atggccccct ggccaccggc gccacagatt caatttctct tactttccct gaaggtatct
     9001 tttatattcc taattctttc cttgctgtac ataatgcacc tgtaaaccct cttcctgtta
     9061 ccgaaatgcc taataatcaa caaatagtaa cctggaaaat tcctcctaac gttcaggtcg
     9121 gcgacagcgt agtttttgaa atcaattatt tttgtcaggc caatgtggtt gagtgcggca
     9181 tttattatat cgatgccatg tcgtatagtt ccagaaacct gctatgccag ctgagcggtc
     9241 agaattgcga tattctggtg agcaccggca acgcgcagat acccatctat atttataaat
     9301 cctacctttc gttatccaat gcatctgcat ggtctgtacc gtctggggca aaccaggaaa
     9361 cggtgtacac ttcctatacc atttccaata atggcgaatc tattattgcc aataatacgg
     9421 ttttcatgag ttatttcaac gatgttgatg gaaatcagat tctttctgct cctgatgttt
     9481 ttttaggggc tgattcaata aatgtttata tcgacaccaa ttctgtcaat ccttttaatt
     9541 cgcatatcac tgttcctgtc ggcagttcct gtaaactcat tttcgcattg gataccaacg
     9601 ccaacggttg tatctgcgaa ctcgatcaga tctttgccaa tgtgcagctg gtaaatgcag
     9661 gacaggatac tgttgt
//
LOCUS       DAOSFP010000035         9612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6043_length_9612_cov_3.673852, whole genome shotgun sequence.
ACCESSION   DAOSFP010000035 DAOSFP010000000
VERSION     DAOSFP010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_9612_cov_3.673852"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLI16_01880"
     CDS             <1..187
                     /locus_tag="PLI16_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83339.1"
                     /translation="TTNNPIEIKLFNVVGTLTRKAEINSSRQQLSIDISNLAPGTYFL
                     KVNENNRYSTKKIMIVH"
     gene            complement(189..527)
                     /locus_tag="PLI16_01885"
     CDS             complement(189..527)
                     /locus_tag="PLI16_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOH83340.1"
                     /translation="MIADYFKGINVAVTVCNEDGTIVYMNEKAALTFQKYGGMALIGK
                     NLLDCHPEPARSKLGKMLITHESNSYTIEKNGIKKMIYQVPRFEDKIFKGYIEFSMEI
                     PFEMPHFIRK"
     gene            complement(529..1203)
                     /gene="nth"
                     /locus_tag="PLI16_01890"
     CDS             complement(529..1203)
                     /gene="nth"
                     /locus_tag="PLI16_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOH83341.1"
                     /translation="MSKKQRYEYFIEYFSKHQPEADTELVYTDPYQLLVAVILSAQCT
                     DKRVNMITPAFFKKFPDAVSLSKATETGVFDLIKSCSYPNNKAKNLLGMAKTLVGEFG
                     GVVPDDIDNLQKLPGVGRKTANVIASVVFKKPAMAVDTHVFRVAARIGLTTNSKTPLE
                     TEKQLVGNIPEKHIAIAHHWLILHGRYICQARKPLCEKCGISTICKYYNAENSNKKVA
                     NNKRVK"
     gene            complement(1266..1532)
                     /locus_tag="PLI16_01895"
     CDS             complement(1266..1532)
                     /locus_tag="PLI16_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83342.1"
                     /translation="MNKTSTLNEILLYCYGESGEMAEYAIENLLESDFGLNENFKILN
                     DTRLEIENSMVNPPDSLISKVIAYSEALTILNVSEPDLKTMIRN"
     gene            1784..2248
                     /locus_tag="PLI16_01900"
     CDS             1784..2248
                     /locus_tag="PLI16_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83343.1"
                     /translation="MKKNPYTTSTREVRNAEIHPKFIKYLKDYAGMYKLGDLENDIQH
                     CFISTNQKKGFFGGLKTNYTVVCFTTEFLFWGIIYDNKESGIGAAKWTEISEVRDWET
                     SEMAALAEAHGVEILCFMYQASRRSRSFIALDQSTAGQRCRDLMKERISEKK"
     gene            complement(2278..2830)
                     /locus_tag="PLI16_01905"
                     /pseudo
     CDS             complement(2278..2830)
                     /locus_tag="PLI16_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            3057..5885
                     /gene="uvrA"
                     /locus_tag="PLI16_01910"
     CDS             3057..5885
                     /gene="uvrA"
                     /locus_tag="PLI16_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOH83344.1"
                     /translation="MELTGIESYSPKEYIIIKNARVHNLKHLDIALPRNKFIVITGLS
                     GSGKSSLAFDTLYAEGQRRYIESLSAYARQFLGRMPKPEVDYIKGISPAIAIEQKVKT
                     HNPRSTVGTSTEIYDYLKLLFARIGTTCSPVSGNKVKKDSITDVVDFVLGCTQGTMVF
                     IECPVHFNKERSVEEHLKTLQQQGFNRLDVQGDIVTIDTLLEKGKKISTKTQINLVLD
                     RVMINEDTVNSRDRIADSVQTAFYEGAGQCIIITSQSEEKKKTIFSDKYELDGIVFEE
                     PSVNLFTFNNPYGACKVCEGFGSIIGIDEDLVVPNKSLSVYEEAIACWKGEKMGEWKD
                     QLVKNAYKFDFPIHKPYIELSEEHKELLWKGNEFFGGINEFFKYVESQTYKIQYRVML
                     ARYRGKTICPDCRGTRLRKDASYVKVAGKSIIDIVLMPVKEALRFFRTISLSDYENQV
                     AKRILIELTTRLQFLSDVGLGYLTLNRASNTLSGGESQRINLATALGSSLVGSMYILD
                     EPSIGLHPRDTEKLISVLKRLRDIGNSVIVVEHDEEIIKASDQIVDIGPLAGMHGGEL
                     IFQGSYEELLQFPDSLTSKYLNKVTAIPVPHFRRPWKKFIEICGAHENNLKNICVKFP
                     LNVLTVATGVSGSGKTTLVKQTLYSSLRKIYGGYSEKTGKHIKLNGNIDNIGNIEYVD
                     QNPIGKSSRSNPVTYIKAYDDIRSLFAEQQLAKLRGFKPGYFSFNIPGGRCEECQGDG
                     YVTIEMQFMADISLTCESCGGKRFKDEVLDITWNGKNINEVLDMTIEQAIIFFNGGSK
                     QHNAGATIANKLQPLVDVGLGYIKLGQPSSTLSGGEAQRLKLASFLLKGSVDKPTMFI
                     FDEPTTGLHFHDIHKLYDAFNALIDKGHTIIVIEHNMELIKCADWLIDLGPEGGNEGG
                     YVVFEGTPEDMLGCKKSYTAKFLKEKLQ"
     gene            complement(5943..7259)
                     /gene="rimO"
                     /locus_tag="PLI16_01915"
     CDS             complement(5943..7259)
                     /gene="rimO"
                     /locus_tag="PLI16_01915"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOH83345.1"
                     /translation="MPDTRLYPREQRKLVINVVTFGCAKNIVDSEKIMGSLPSRKFKI
                     IHNSGQQADIVIINTCGFINDAKQESVDAILEFTEAKKVNNTKVFITGCLAQRYKLEL
                     LAEIPDLDGVFGLNETNALLREITNADTKQSDNRILTTPKHYAWLKISEGCDRKCSFC
                     AIPMIRGVNISYPIEKILDEAVMLADKGVKELMVIAQDTTYYGLDIYKKRKLAVLLEK
                     LSDIRQFEWIRLHYAFPAGFPEDVLELMQSRDSLCKYLDIPLQHIDDKILASMRRGID
                     KKGTVALVEKIRKKVPGIALRTAFIVGYPGETRAQFDALCNFVREQQFERMGVFTFSK
                     EEGTVAGAIKQQTGAKEKQRRYEQLMELQQQISASKNQALVGKTMKVIIDRKENEFYI
                     GRTEFDSPEIDNEVLIPVKDKVLKSGSFYDVRITGSEVYDLYATLK"
     gene            complement(7252..8208)
                     /gene="ftsY"
                     /locus_tag="PLI16_01920"
     CDS             complement(7252..8208)
                     /gene="ftsY"
                     /locus_tag="PLI16_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOH83346.1"
                     /translation="MGIFSFFSKDKKESLNEGLAKTKESFFSKLSKVVIGKSKVDDEV
                     LDNLEEILISSDVGVATTLKIIGRIENRVAKDKYLGTKELDQILKDEITALLTENNNE
                     EFGDFDIPSGKKPYVIVVVGVNGVGKTTTIGKLAHQFKAKGNQVLLGAADTFRAAAVD
                     QLKIWSDRVGVPVVSQGMGADPASVAFDAVSSAKTRNADVVIIDTAGRLHNKVNLMNE
                     LSKIRKVIQKVIPDAPHEVLLVLDASTGQNAIEQARQFTAATEVNAIALTKLDGTAKG
                     GVVLGISDEFKIPVKYIGVGEKIDDLQIFNKVEFVDSFFQHA"
     gene            complement(8280..8435)
                     /locus_tag="PLI16_01925"
     CDS             complement(8280..8435)
                     /locus_tag="PLI16_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOH83347.1"
                     /translation="MAKKVVATLKTSTGKDYAKVIRMVKSPKTGAYSFKEEIVANDKV
                     KEYLAKK"
     gene            complement(8448..8636)
                     /gene="rpmG"
                     /locus_tag="PLI16_01930"
     CDS             complement(8448..8636)
                     /gene="rpmG"
                     /locus_tag="PLI16_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOH83348.1"
                     /translation="MAKKSKDARIQVILECTEHKNSGMPGTSRYITTKNKKNTPERIE
                     LKKYNPILKKVTVHKEIK"
     gene            complement(8806..9423)
                     /locus_tag="PLI16_01935"
     CDS             complement(8806..9423)
                     /locus_tag="PLI16_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOH83349.1"
                     /translation="MKKITVIFVLLACVVALNSCKKIAGCTDQAAQNYNADADEDDGS
                     CTYLVIGDSYQGGKIAYILQPGDNGYDAKVQHGLIAAPSDQDTAAGWGCAGTMISGAD
                     GTAVGTGNQNTIDIVAGCSAAGTAARICSDLGLGGYTDWYLPSKDELQKLYVNRAQIG
                     GFATSFYWSSSEATANEAWWYSFFDGGAYAYSKSTPIRVRAVRSF"
BASE COUNT         2791 a         2055 c         1914 g         2852 t
ORIGIN      
        1 aacaacaaac aacccgatag aaataaaact cttcaatgtg gttggaacac tgaccagaaa
       61 ggccgaaatt aatagcagcc ggcagcagct aagtattgat atttcaaatc tggccccggg
      121 aacctacttc ctgaaagtta acgaaaacaa ccgttattca acaaaaaaaa taatgattgt
      181 acattgacct actttcggat aaaatggggc atttcgaacg gaatttccat tgaaaactca
      241 atgtatccct taaaaatttt atcctcaaac cggggcacct ggtaaatcat ctttttaata
      301 ccgttttttt cgatagtata actgtttgat tcgtgtgtta tcaacatttt gcccagctta
      361 cttcgcgccg gttcggggtg acagtccagc aaattttttc caatcagggc catgccgcca
      421 tatttttgaa aagtaagggc agccttttcg ttcatatata caattgtccc gtcttcgttg
      481 catacggtaa cggcaacatt gattccttta aaatagtcgg caatcatttt atttcactct
      541 tttattattt gcaactttct tattactatt ctctgcatta taatacttgc agatggtgct
      601 tattccgcat ttttcgcaga gaggcttcct ggcctggcat atataccgtc catgcaatat
      661 cagccagtga tgagcaatag caatatgctt ctcgggaata ttacccacca gttgtttttc
      721 tgtttccagc ggggttttac tgttggtcgt gaggccaata cgtgcagcca ccctgaaaac
      781 atgggtatcc acagccatag ccggcttttt aaacaccacc gacgcaatta cattggcggt
      841 tttacgacct acacccggta atttttgcag gttgtcaata tcgtcaggca ccacaccgcc
      901 aaattcgcca accagggttt tggccattcc caacaggttt ttcgccttat tgtttggata
      961 tgagcaactc tttatcaaat caaaaacccc tgtttcggtt gcttttgaaa ggctgacagc
     1021 atcggggaat tttttaaaaa atgccggcgt tatcatgttc actctcttgt cggtgcattg
     1081 ggcagacaaa ataacagcaa caagcagttg gtaaggatcg gtataaacca gttcggtatc
     1141 ggcttcaggc tggtgttttg aaaaatattc aataaaatat tcgtaacgct gtttttttga
     1201 aatcataggt ctcattaaaa aaacaaagat aaaaaaagcc cctgattttt caagagcttt
     1261 tccgtttagt tgcggatcat cgtcttcagg tcaggctccg atacatttaa tatcgttagc
     1321 gcttccgagt acgcaattac tttactaatc agggagtcag gcggattcac catactgttt
     1381 tcaatctcga gtctggtgtc gtttaaaatt ttgaaatttt cattcagacc gaaatcggat
     1441 tccagcaggt tttcgatggc atattccgcc atttcgccgc tttctccgta acagtaaagt
     1501 aaaatttcat taagggtcga agttttattc ataggctatt ttggtttatt tgtactttaa
     1561 ctaataagtt aacgcctgcc tggtattcaa tattgtatgc cggataaaaa attattttcc
     1621 gcctttattt ccggcaaaat caattgtcat tggctacttg aagccaatct tttgaaaaat
     1681 acatggtatt tattgtggag tggaatttat aggtgcaagt ttttttcctc aagtatcctt
     1741 ataaatacaa taatttttaa taaatttgag aaaatataaa gccatgaaaa aaaatccata
     1801 taccacttct acacgcgaag taagaaatgc tgaaatacat ccgaaattca tcaaatacct
     1861 aaaagattat gccggaatgt ataaactcgg ggatttggaa aacgacattc agcattgctt
     1921 tatcagtaca aaccaaaaga aaggtttttt tggcggcttg aagacaaatt acaccgtagt
     1981 ttgtttcacc acagagtttt tattttgggg catcatctat gataacaagg aaagcggtat
     2041 cggggctgcg aagtggactg aaatttcgga agtcagggat tgggaaacat ccgaaatggc
     2101 cgctctggca gaagctcatg gcgtggagat tctatgcttt atgtatcagg ccagccgccg
     2161 gagccgttca ttcatcgcac tggatcaatc tacggcaggc caacgctgcc gcgacttaat
     2221 gaaagaaaga atttctgaaa aaaaataaaa acactgacca gggcaagtcc gggaaagtca
     2281 ggccgtcaag atcaggtctt tttgttcgat aagttttctc aggttgataa gcgcataccg
     2341 catacggccc aaagccgtgt taatgctgac attggttgat tcggcaatat ccttaaaact
     2401 catgtcgtaa taatgacgca taatcagcac ctctttttgt tcagccggaa ggtactcaat
     2461 cagcgaacgg acatcattgt gtatctgctc atgaaccatt ttatcctcaa tggaatagtc
     2521 gaggattttc aaggaatcaa aaatatcgtt ttgattttct gaaatgatca ataataagat
     2581 tgtgggctat acgcatggcc cactgaagaa attttccctc ttctttataa gcacctgaac
     2641 gtaatgtatt tacaacctta acaaaagtat cctgaaaaat atcctccgca aggtggttat
     2701 ccctgacaat catcaggata taggtaaaaa ctcttttttt gtgccggttg atgaggactt
     2761 caagacattg atggttaccg gataaataac tatcaattaa tgcctggtcg ttatcagact
     2821 gaaaggacat atttacctcc ttttttaaag tttaaaaaat ttcaaggtaa aatgtctatt
     2881 cgataaaagt cctccagttt gggttaaaac ttaattattt aacgacgaaa tgaaaaaatc
     2941 aattatttgg tcaataatta agcacaaaga tatatcattt ttgaactgaa tgcaagactt
     3001 attttgtaat tttgataaat tttatgattt tttaagtttc aaattttgaa tttttaatgg
     3061 aactaacagg aattgagtcg tatagcccaa aagaatatat cataatcaaa aatgcccgtg
     3121 ttcacaacct aaaacacctg gacattgctc ttccgcgaaa caagtttatt gttattaccg
     3181 gactttccgg ctcagggaaa tcatcgcttg cttttgacac tttatatgct gaaggccaaa
     3241 ggcgctacat cgagagtctg agcgcatacg ccaggcagtt tttggggcgc atgccaaaac
     3301 ctgaagtgga ctatatcaaa ggcatttcgc ccgccatcgc tatcgagcaa aaggtaaaaa
     3361 ctcacaatcc ccgctctacc gttggaacgt ccacagaaat ttatgattac ctgaaactgc
     3421 tttttgcgcg gattggcact acctgttcac ccgtatcagg caacaaagta aaaaaagaca
     3481 gcattaccga cgtggtggat tttgttctcg gctgcactca gggaactatg gtattcattg
     3541 aatgtccggt acatttcaac aaagaacgtt cggtggaaga acacctgaaa accttgcagc
     3601 agcaaggatt taaccggctg gatgtacagg gcgatatagt aaccattgat accttactgg
     3661 aaaaaggaaa gaaaatcagc accaaaactc agattaacct ggttctggac cgcgtgatga
     3721 ttaacgaaga caccgtgaac agccgcgacc gcattgctga ttcggtgcaa actgcttttt
     3781 acgaaggcgc aggacaatgc attattatca cctcccaatc agaagaaaaa aagaaaacca
     3841 tcttttcgga caagtatgaa ctagatggaa ttgtattcga agaaccttcg gtcaatttgt
     3901 ttaccttcaa caatccttac ggcgcctgca aagtctgtga aggttttgga agcattatcg
     3961 gcattgacga agaccttgta gtgccaaaca aatcgctctc ggtttacgag gaagccattg
     4021 cctgttggaa aggcgaaaaa atgggcgaat ggaaagatca acttgtaaaa aatgcttata
     4081 agttcgactt cccgatacat aaaccttaca ttgaactttc ggaagaacat aaagaactgc
     4141 tgtggaaagg taatgagttt tttggcggaa tcaacgagtt ttttaaatat gtcgaaagtc
     4201 agacctacaa aatccaatac cgtgtgatgc tggcgcgtta tcggggaaaa accatttgtc
     4261 ctgattgcag gggcacacgc cttcgcaaag atgcctccta tgtaaaagtc gccggaaaat
     4321 ctataatcga catcgtactg atgccggtaa aagaagcatt aagatttttc cggaccatat
     4381 ccttaagcga ttacgaaaac caggtggcaa aaaggatact tattgaactt acgacccgtc
     4441 ttcaatttct gtcagatgtg ggccttggct atttaacact aaaccgggca tccaacacct
     4501 tgtcgggagg cgaatcgcag cgtatcaacc tggcaacagc actcggaagc agcctggtag
     4561 gctccatgta catacttgac gaacccagca tcggtcttca tccgcgcgat accgaaaagt
     4621 taatctccgt gctgaaacgc ttgcgcgaca ttggcaacag tgttattgtg gttgaacatg
     4681 acgaagaaat cataaaagcc tcggaccaga ttgtggatat agggccactg gcaggtatgc
     4741 acggcggtga attaatattt cagggaagtt atgaagaatt gctgcagttc ccggattcac
     4801 tgacatcaaa gtacctcaac aaagtaaccg cgatacctgt tccccatttc cgcaggccct
     4861 ggaaaaaatt tattgaaatt tgcggagctc acgaaaacaa cctcaaaaat atctgtgtta
     4921 agtttccgct caatgtcctg acggttgcta caggagtaag cgggtcggga aagaccaccc
     4981 tggtaaagca aacgctgtat tcctctttaa gaaaaattta tggcggatat tctgaaaaaa
     5041 caggcaaaca tataaaactc aatgggaata tcgacaatat tggaaatata gaatatgttg
     5101 accaaaaccc cattggaaaa tcttccaggt cgaatccggt aacttatatc aaagcttacg
     5161 acgatatccg aagcctgttt gccgaacagc aacttgctaa attgagagga tttaaacccg
     5221 ggtatttttc tttcaacatt ccaggtggac gttgcgaaga atgtcagggt gatggctatg
     5281 taaccattga aatgcagttt atggcagata tttccctgac ttgcgagagt tgtgggggaa
     5341 aacgtttcaa agacgaagtg ctcgacatca cctggaacgg aaaaaatatc aacgaggtac
     5401 ttgatatgac tattgagcaa gcgataatat tttttaacgg aggaagcaaa caacataatg
     5461 ccggggccac tatcgcaaac aaactacaac cattggttga tgtcgggctg ggatatatta
     5521 aacttggcca gccgtccagt acgcttagcg gaggcgaagc acaacgcctg aaattagcca
     5581 gcttcctttt aaaaggcagc gtggacaaac ccactatgtt tattttcgac gaacccacca
     5641 cgggcctgca ttttcacgac atccataagc tgtatgatgc ctttaatgcc cttattgaca
     5701 aaggccacac catcattgta attgaacaca atatggaact tatcaaatgc gccgactggc
     5761 tcatcgatct gggccccgaa ggcggcaacg aaggtggcta tgtggtattt gaaggtactc
     5821 ccgaagatat gcttggctgt aaaaaatcat atacagcaaa gtttttgaaa gaaaagctgc
     5881 aatgaccggt atgggaggta accggccctg atccattaat cattacggca gagcaccatt
     5941 tattatttca atgtggcata caggtcataa acctctgagc cggtaattct tacatcataa
     6001 aagcttccgg atttgagtac cttatccttg accggtatca gcacttcatt atctatttcc
     6061 ggagaatcaa attccgtacg gccaatgtag aactcgtttt ctttacggtc aatgattact
     6121 ttcatggtct tacccaccaa tgcctgattt ttcgatgcag aaatttgctg ctgaagttcc
     6181 atcagttgtt cataacggcg ctgtttctcc tttgccccag tctgctgttt gatagcgccg
     6241 gcaactgttc cttcttcctt tgaaaaggta aatacaccca tacgttcaaa ctgctgctca
     6301 cgtacaaagt tacagagcgc atcaaattgc gccctggttt caccggggta acccacgatg
     6361 aaagccgtac gtaaagcgat gcccggaacc ttctttcgga ttttttcgac caatgcaacg
     6421 gtaccttttt tatctatacc cctgcgcatc gaggcaagga ttttatcatc gatatgctgc
     6481 aacgggatgt ccagatactt acataaacta tccctgcttt gcatcagttc cagcacatct
     6541 tcagggaaac ctgccggaaa agcataatga aggcgtatcc actcaaattg ccttatatca
     6601 gagagttttt ccaacaatac cgctagtttc cgttttttat aaatatcgag tccgtaatag
     6661 gtagtatcct gtgcaattac cataagctct ttcacacctt tatcagcaag catgacagct
     6721 tcatccagga ttttttcaat aggatatgaa atattaaccc ctcgtatcat cggaatggca
     6781 caaaaagaac atttgcggtc gcatccttcg gagattttga gccaggcgta atgtttcggg
     6841 gtggtaagta ttctgttatc cgattgtttt gtgtcagcat tggttatttc cctgagtagc
     6901 gcattcgttt cattcaagcc gaaaacgcca tctaaatccg gaatttcagc aagcagttcc
     6961 aatttgtagc gttgagcaag gcatccggtt ataaaaactt tggtattatt tacttttttg
     7021 gcttcggtaa actccagaat tgcatccacc gattcctgtt ttgcgtcatt aataaagccg
     7081 caggtattta ttataacaat gtcggcctgt tggccggaat tatgtattat tttaaatttc
     7141 cgggagggca ggctgcccat tattttttcg gaatcgacta tgtttttagc gcacccgaaa
     7201 gtaacgacat tgataacaag tttacgttgt tcccggggat atagtcttgt gtcaggcatg
     7261 ctgaaagaaa gaatctacga actccacctt gttgaatatc tgtaagtcat caattttttc
     7321 gcccactcca atatatttaa cagggatttt aaattcgtct gaaataccca ggaccacacc
     7381 gccctttgct gttccgtcga gttttgtaag cgctatggcg ttgacctcgg tggcggcagt
     7441 aaactgccgt gcctgttcta tcgcattctg gccggtagag gcatccagca ctagtaatac
     7501 ttcatgcgga gcatcgggta tgactttttg tattactttt ctgattttcg acaactcgtt
     7561 catcaggttt actttgttgt gtaagcggcc agccgtgtcg ataatcacca catccgcatt
     7621 acgggttttt gctgaagaca ctgcatcaaa agcgacagaa gcagggtcgg ctcccattcc
     7681 ctggctaact acgggcactc ccaccctatc agaccagatt tttaactgat ccaccgctgc
     7741 ggcacgaaaa gtatcggcgg cacccagcaa tacctgattc cctttggctt tgaactggtg
     7801 tgctaattta ccgatagtgg tagttttgcc tacgccgtta acaccaacca ccacaatcac
     7861 ataaggtttt tttcctgaag gaatatcaaa atcgccaaat tcttcgttgt tgttttctgt
     7921 cagcaaggcc gtaatctcat ctttgagtat ctgatccagt tctttggttc ccagatactt
     7981 atccttagcc acccgatttt cgatacgtcc aataattttg agagtggtag ctacaccaac
     8041 atcggaggat atcagaattt cctcgaggtt atccagcact tcatcatcca ctttggattt
     8101 gccgataacc actttggaaa gctttgagaa aaaactttct ttggttttgg caaggccttc
     8161 gttgaggctt tccttcttat ctttagagaa aaaactgaat atacccattt tcgattatgt
     8221 taaaaaaaaa caagcttctc cgacagaaaa gcttgtttct tttcatatca gatgaatgat
     8281 tatttttttg cgaggtattc tttaacctta tcgttggcta caatttcttc tttaaaggaa
     8341 taggctcctg ttttgggaga tttcaccata cggataacct ttgcataatc cttacctgta
     8401 gatgttttta acgttgcaac aactttcttt gccatttctt aagagtttta ttttatttct
     8461 ttatgaacag taaccttttt cagtatcgga ttatattttt tcaattcgat acgttccggg
     8521 gtgttttttt tgtttttggt tgtgatatag cgtgatgtcc ccggcatacc gctgttttta
     8581 tgctcagtac actccagaat aacctgtatg cgtgcatctt tcgatttctt tgccataata
     8641 tcttgatttt tattttaagg gtgcaaagat actaatttta ttaatcctaa aaaatattta
     8701 tcgaaaaaaa attattaaaa actcatacaa tcctttaatt gtctgttttt tgacattgtt
     8761 caaaggattg tatgagtcaa atgtccgtct gatcatcctc cggatttaga aagacctcac
     8821 agcccttaca cgtatcggag tagatttgct gtatgcatag gcacctccgt caaaaaatga
     8881 ataccaccag gcttcattag cggtagcctc agaggaactc cagtaaaagg aggtggcaaa
     8941 gccgccaatt tgagctctgt ttacgtataa cttctgtagt tcgtctttgc tgggaagata
     9001 ccagtcggta tatccgccca aaccgaggtc ggaacaaatt cttgcagcag ttccggccgc
     9061 tgaacaaccg gcaacaatat caatagtatt ttggttgcct gttcccaccg cggtaccatc
     9121 agcgccgctt atcatagtac cagcacatcc ccaccctgca gcggtgtcct gatcacttgg
     9181 ggctgctatg agtccatgtt gtactttggc atcgtaaccg ttatcaccgg gctgcagaat
     9241 atatgctatt ttgcctccct gataactgtc gccaatgacc aggtaagtgc atgaaccatc
     9301 atcctcgtca gcgtcagcat tataattttg ggctgcctga tcggtgcatc cggcaatttt
     9361 tttacagctg ttgagtgcaa caacacaggc cagcagaaca aaaataacag tgattttttt
     9421 cattttttca aatttaaggg tttaaattat atttcttatt taaatcagga ttttatcaac
     9481 caatctttaa taacttttca ataaaaactc ataatatctt actataaatt caatcatcaa
     9541 tcctttaaat aaatagttta atgaacgagt ttcagcacca tatattttga ggtattggca
     9601 ccagtgtatt cc
//
LOCUS       DAOSFP010000036         9430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6185_length_9430_cov_4.158720, whole genome shotgun sequence.
ACCESSION   DAOSFP010000036 DAOSFP010000000
VERSION     DAOSFP010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6185_length_9430_cov_4.158720"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..312)
                     /locus_tag="PLI16_01940"
     CDS             complement(91..312)
                     /locus_tag="PLI16_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83350.1"
                     /translation="MMKLLLPAIILLAIAFAGFAIKVIVKKNGEFKKSCSTIDPKTGK
                     PFGCTCGSSGKDQTQCENFNEHHPGEAGK"
     gene            complement(394..1140)
                     /gene="deoC"
                     /locus_tag="PLI16_01945"
     CDS             complement(394..1140)
                     /gene="deoC"
                     /locus_tag="PLI16_01945"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOH83351.1"
                     /translation="MNQDASNDKTKKEESAKDTLKNMAISQAELASYIDHTLLKPEST
                     REQFVRLCEEAVTYRFKSVCVNSFWVPFVTGILKGSGIKICSVIGFPLGAMDSSSKAF
                     ETGSAISAGAEEIDMVINVGALKSQDFATVEKDIQAVRNACQGGALLKVIIETVFLDE
                     KEKIQACEISKQCGADFVKTSTGFMGGGATVEDIALMRKVVGPDLGVKASGGIRNFEQ
                     AVSLIHAGANRLGCGSSVAVITGAPVKGNY"
     gene            1421..2191
                     /locus_tag="PLI16_01950"
     CDS             1421..2191
                     /locus_tag="PLI16_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83352.1"
                     /translation="MMKDKTKLLVIISLILNIGIIGTLLFVKNMVESRLSKVSGMLTR
                     LGNSEIDYTVHVEDTLEISTAFSIKEVVPVFVEMRVKYDLPFKAMIPVDQQIETPITL
                     TVDKTIHLDTVFMFKNKIVAPLDEIISVDQMFTIPTNREKKKGINIPIVADIPLKQTI
                     SLDIKDPISVLADIPIKIPISETIPVKIELTIPVDINVPIDIPVKSEALITFPYQLPI
                     SGKIPLNLDIPVKIPLSATPIKSKSDSIAYELERLLRF"
     gene            complement(2212..3252)
                     /locus_tag="PLI16_01955"
     CDS             complement(2212..3252)
                     /locus_tag="PLI16_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOH83353.1"
                     /translation="MDFTLLIIIIILAFAFDFVNGFHDAANSIATIVSTKVLTPLQAV
                     IWAASFNFIAYAIFELHIVDTISKTVDTSVINLQVILAGLIAATIWNLLTWYLGIPSS
                     SSHTLVGGFAGAAVAYGGWSIVHSGVILKIGAFIFLAPILGMVISYLFSILMLHITKK
                     ARPAKVHKVFRPLQLVSSALFSLGHGGNDAQKVMGIITAAIIVYLKGVDPADVPHWAH
                     ITFTSAGKIHAIPHWIVLGCYTCIAAGTLLGGWKIVKTMGSKITRLTPFEGFAAETSG
                     AMLLFGTEAFGIPVSTTHTITGAIIGTGMTKRLSAVRWGVGKQLLVAWILTIPVSMLI
                     AMGVYHLVMLFY"
     gene            complement(3271..3918)
                     /locus_tag="PLI16_01960"
     CDS             complement(3271..3918)
                     /locus_tag="PLI16_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOH83354.1"
                     /translation="MQVNNLLKFFTPRERKFYPMFEEAAENLVKGATMLNKYFLVSDP
                     VEKEKILVSIKEHEHLGDNITHKLYDELNKTFITPFDREDIYKLTGKLDDVMDLINRF
                     GQNVKNHPPKKEPKSFLELSELILQATQQIRVAIYELKNLKVPKKITDACIRINEIEN
                     QADDLYYQAIADLFNSEKDAIELIKVKGMLSALEKATDSAEDVSDVLKSIIVKTT"
     gene            4159..4845
                     /locus_tag="PLI16_01965"
     CDS             4159..4845
                     /locus_tag="PLI16_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH83355.1"
                     /translation="MDNEPIILLVDDEPDIIEFLSYNLKKEGFKVYTADNGKDGIKTA
                     KETLPHLIVLDVMMPGMDGVETCQELRKIPALSKTLIMFLTARGEDYSQIAGLDAGAD
                     DYITKPIKPQLFISRVNALLRRHSEQTTPGNSTIKISDLLIDREQFIVLKQGQKIVLP
                     KKEFELLYLLASKPSRVFTREEILSKIWGDNVVVGDRTIDVHIRKVREKIGMEFISTV
                     KGIGYKFENE"
     gene            4842..5879
                     /locus_tag="PLI16_01970"
     CDS             4842..5879
                     /locus_tag="PLI16_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH83356.1"
                     /translation="MKKHSPKNISLEIAFYVMLVFFVLFLVYYLIFQGHHSDWLIILI
                     IGPLFFGAVYLIIRHSVENFIYEKIRVLYKTLHNLKTPKQKTKIEEHWFENAEAEIEK
                     WVEDKKNELESLRKSENYRRDFLGNVSHELKTPIFNIQGYILTLIDGGLDDKSINREY
                     LLRAEKSIDRMIAIVNDLDVIAQLESETMKMEMEKFDLTQLAHDVFETYEIKGKQKNI
                     SFILHNKLSGIVHVMADKEKIRQVLNNLIENAIKYGKQDGRVKVTFYDMDEMVLVEVS
                     DNGIGISQEDIPRIFERFYRTTQGKSLENRGSGLGLAIVKHIIEAHGQTINVRSMLGA
                     GTTFGFTLQKA"
     gene            6100..6972
                     /locus_tag="PLI16_01975"
     CDS             6100..6972
                     /locus_tag="PLI16_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOH83357.1"
                     /translation="MKNARYNNKIIKIRQRIYEIIFEADTKAGKLFDLGILTIILLSI
                     FLVILESISSIRMQYGHILRILEWGITVVFTLEYLARIWVTEKPLKYIFSIYGIIDLL
                     ALLPTYLDVFFVGGHNMIVIRALRLLRIFRIFKLHRYTKAGRTIASALWSNREKISIF
                     ILFVFTIAIIIGTVMYLIEGEQNGFTDIPTSIYWAIVTLTTVGYGDLSPSTGIGQFLA
                     SVVMVLGYSIIAVPTGMVTATLIGQKKVTNTQVCSRCMYDKHDDDANYCKICGTSLHP
                     NSESRKNNTTTDLD"
     gene            complement(7014..8339)
                     /locus_tag="PLI16_01980"
     CDS             complement(7014..8339)
                     /locus_tag="PLI16_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH83358.1"
                     /translation="MNKIFIIIKREYLSRVKKKSFIVMTILGPLLMAALMIVPYYVTK
                     LDREQQVIDVLDETGIYYKMGSNETIKFNYIYTDLQTAIAGFDGDKSAALLYIPKPDM
                     LEPTNAILYYSDKQPGNTVTSYIKDKMKYIYENRKLMELYGIDKKQIDEAKVRININS
                     QNIETGETNLPIATTIMGIFAGFLIYFAIFMYGNQVMRGVIEEKTSRIVEIIVSSVKP
                     FQLLLGKIIGIALVGLTQFLLWIVLTLSLVAVFQVSVGAKDILSKPQTETVQQGNVLV
                     PENNTPAPVQDYDDESVRSLVKSFGEYNYSLIIFSFFFFFLFGYLLYAALFAAIGSAV
                     DNEADTQQFMLPITIPLILAFVLAQPIIENPQGPVAFWFSVIPLTSPIVMMIRIPFGV
                     PAWEFILSMALLILGFLACTWLAAKIYRTGILMYGKKVSYRELWKWLKY"
     gene            complement(8345..9295)
                     /locus_tag="PLI16_01985"
     CDS             complement(8345..9295)
                     /locus_tag="PLI16_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH83359.1"
                     /translation="MNVLETRNITKRFANHVALNGVSISVPEQSIFGLLGPNGAGKTT
                     LIRIINMITGPDEGEVFLKGEKLEKNHIVNIGYLPEERGLYKKMKVGEQAIYLAQLRG
                     LTYSDAIRKLRYWFEKFELMSWWNRKVEELSKGMQQKIQFIVTVLHEPKLLIFDEPFS
                     GFDPINASLIKDEILRLRDNGATVVFSTHNMASVEELCDDIALINKSNKILEGKVNEI
                     KYSYRSFSYQVEIAGTGASLKERLNKDFEVYDEVLQDGLLKAKIKILNNKTTNDMLSA
                     LMPHGTIHALHEIVPTMNDIFIAKVNADINLNYSSSGLTE"
BASE COUNT         2814 a         2020 c         1869 g         2727 t
ORIGIN      
        1 acctgcgaca acttaaagcc caagggccaa cctacttcaa acgctccggc aataagaaga
       61 taaatccatt ccatagttca acaataatca ttatttgccg gcttccccgg ggtggtgttc
      121 attaaaattt tcgcattggg tttggtcttt tccactactg ccgcaagtgc acccaaaagg
      181 tttgccggtc ttggggtcga tagtggaaca tgatttttta aactcgccgt ttttcttcac
      241 aattaccttg attgcaaatc cggcaaaagc gatagccaac agaattatag cagggagcaa
      301 cagtttcatc atgcgagtat ttgataggta gtatttatgt ccgggaagtt tttcacgtca
      361 attcccgata cttttttttt gccctcatat atatcaataa tttcctttta cgggcgcgcc
      421 ggtaataact gccacgctgg aaccgcatcc caacctgttg gcgccggcat gtatcaaact
      481 caccgcctgt tcaaaattcc tgatgccgcc gctggcttta acacccagat cgggtcccac
      541 cacttttcgc atcaaagcga tatcttcgac ggtggctccc ccacccataa aaccggtact
      601 tgtcttaaca aaatcagcac cgcattgttt tgaaatttcg catgcctgaa tcttttcttt
      661 ctcatcaaga aataccgttt cgatgattac tttcagcagg gcgcctccct ggcaggcgtt
      721 gcgcacagcc tgaatatctt tttccacggt ggcgaagtct tgtgatttca atgcgcccac
      781 attgataacc atgtcaattt cttcggcgcc ggcggatatc gcgctgccgg tttcaaaagc
      841 cttgctgctg ctgtccatag cgcccagagg aaacccgata acagaacaaa tttttattcc
      901 tgaacccttg agaataccgg taacaaaagg cacccagaat gagttaacac ataccgattt
      961 gaaacggtaa gtaacagcct cttcgcacaa acggacaaat tgctcacgtg tggattcagg
     1021 tttcagcaga gtatgatcga tatatgaggc cagctctgcc tgcgaaatcg ccatattttt
     1081 taaagtatcc tttgctgatt cttccttttt tgttttgtca ttcgatgcat cctgattcat
     1141 tttttcctcc atttttaaat gataatttta taattgcatt tcttttcaag gttggcaaac
     1201 ccaacattta ttccaatagg cgacagcctg aaactccgtg aaaaatcaat tgtttacata
     1261 attactgcag actatttatt aaggtacaaa tataaaaaac aattttcaga gggtaaattt
     1321 tattttgtct tatgccggtt gattttgatt tatgtgataa ctgatttttt tctaatttcg
     1381 ggcaaaaatt ttacgaaaaa aaaaactaac gctgtactat ttgatgaaag ataaaacaaa
     1441 acttctggta ataatttcgt tgatattgaa tattggtatc attggtacgt tgctttttgt
     1501 aaaaaacatg gttgagtcgc gcctgtcaaa ggtttcgggg atgcttacac ggcttggcaa
     1561 ttcagaaatt gattacacgg tacatgtaga ggatacgctg gaaatatcca ccgcattttc
     1621 cattaaagaa gtggttccgg tatttgtaga gatgagggta aaatatgacc ttcctttcaa
     1681 agctatgata cccgttgacc agcagataga aacgcctatc actctcacgg tggataaaac
     1741 catacacctc gacacggtgt ttatgtttaa gaacaagatt gtggctcctt tggatgaaat
     1801 aatttcggtt gaccagatgt ttaccattcc caccaaccgc gaaaagaaaa aaggcatcaa
     1861 tattccaatt gtagccgata ttccactcaa gcaaaccatc agtttagata tcaaagatcc
     1921 tatcagtgtt cttgccgaca ttccaataaa aataccgatt tcagagacta tccccgtaaa
     1981 gattgaattg accatacctg tcgatatcaa tgttcctatt gacatcccgg taaaaagcga
     2041 agcattgatt acttttcctt accaattgcc tatttcgggc aagataccgt taaatcttga
     2101 tattcctgta aagataccgc tatcggctac gccaatcaaa tcaaagtcgg attctattgc
     2161 ttacgaatta gaaagattac tcaggtttta accggaaaaa agtttttctc tttagtaaaa
     2221 aagcataacc agatggtaca ctcccattgc gataagcata ctaaccggaa tagtcaatat
     2281 ccaggctacc agaagctgtt tgcccacgcc ccatcttact gcagacagcc gcttggtcat
     2341 tcccgttcca atgatagcgc cggtgattgt atgagtggtg ctgacgggta ttccgaaagc
     2401 ttcggttccg aacagtagca tggcgccgga ggtttcggct gcgaatcctt cgaatggcgt
     2461 gaggcgggta atttttgagc ccattgtttt cacaattttc cagccaccaa gcaaagttcc
     2521 ggctgcaatg caggtataac atcccaatac tatccagtga ggaatagcat gaatttttcc
     2581 tgctgaagta aacgtaatat gcgcccaatg gggaacatcg gcggggtcca caccttttaa
     2641 atacacgatt atggcggcag taataattcc cattactttt tgcgcatcat taccgccatg
     2701 tcccaaacta aaaagtgctg aagaaacgag ctgaagcggc ctgaatactt tatgaacttt
     2761 tgcaggcctg gcctttttgg taatatgaag cataagaatc gaaaataagt acgagatgac
     2821 catgcctaaa atgggcgcaa gaaaaataaa ggcaccaatt ttcaaaataa caccgctatg
     2881 cacaatactc catccgccat aggccacggc ggcgccagca aatccgccaa ccagggtatg
     2941 cgaagaactc gacggtatgc ccaaatacca tgtcaacagg ttccagatag tcgcagcgat
     3001 aagccctgca aggattacct gaaggttaat aacgctggta tcaactgttt ttgaaatggt
     3061 atcgactata tgcaattcaa aaattgcata agcgataaaa ttaaaagagg ccgcccaaat
     3121 tacagcttgc aaaggcgtga gcactttggt ggatactatg gttgcaattg agtttgccgc
     3181 atcatgaaaa ccatttacaa aatcgaacgc aaaggccaag atgataataa taattaataa
     3241 tgtgaaatcc ataaacctgt gttttgattt ttatgtcgtt tttacaataa ttgatttcag
     3301 cacatcgctc acatcctcgg cgctgtcagt ggctttttcg agcgctgaga gcatgccctt
     3361 taccttaatg agttcgatgg cgtctttctc gctgttgaac aggtcggcta tcgcctgata
     3421 ataaaggtca tcggcctggt tttctatttc gttgatacgg atacaggcat cggtgatttt
     3481 ttttggcact ttaagatttt tcagttcata tatggcaacc cggatctgtt gtgtagcctg
     3541 caaaatgagt tcgcttaatt ccagaaaaga ttttggttct tttttgggag ggtgattttt
     3601 tacattttgt ccaaacctat tgataaggtc catcacatca tcgagctttc cggtaagttt
     3661 gtaaatgtcc tcccggtcga agggagttat gaaagttttg ttcaattcat catataattt
     3721 atgtgtaatg ttatcgccca aatgctcgtg ttctttaata gagacaagaa ttttttcttt
     3781 ttcgacaggg tccgaaacaa gaaaatattt gttcagcatg gtagcgccct taaccagatt
     3841 ctcggcagct tcttcaaaca tagggtagaa tttccgctct ctgggcgtaa aaaacttcaa
     3901 aagattattg acttgcataa attgtattta ttatttttaa acctgataca aaattactac
     3961 cgcgaaaccg aatatcgcaa aaacacagtt tacatttcgg ttaaggttat attaagttaa
     4021 tgttaagtct tgcattggaa aagtaacatt caggtttttc agctcactat atcagattcg
     4081 ggacacaatg gaaatcacca ctcctgactt tttataaaat gaaatacttt tatacctttg
     4141 taaaaacaca aatacactat ggataatgaa cccattattc ttctggtaga tgacgagcca
     4201 gatatcattg aattcttaag ttacaattta aaaaaagaag gttttaaagt ttatacagcc
     4261 gacaatggta aagacggcat aaaaaccgca aaagaaacgt tgccacacct gattgtactt
     4321 gatgtgatga tgcccggaat ggatggcgtg gaaacctgcc aggaattaag aaaaatccct
     4381 gctctttcga aaacgctgat catgtttctt acggcacgcg gagaagacta ttctcaaatt
     4441 gcaggactgg atgccggcgc cgatgactat attacaaaac ccataaaacc gcaactattt
     4501 atcagtcgcg tcaatgcttt gctgcgcagg cattccgagc aaacaacacc aggaaattca
     4561 accataaaaa tttcagacct tttaattgac agagaacaat ttattgtcct gaaacagggg
     4621 cagaaaatag ttctccccaa aaaagaattt gagcttcttt atctgttggc ttcaaagccc
     4681 agcagggtat tcacgcgcga agaaatttta tcaaaaatat ggggtgataa tgtggttgta
     4741 ggcgaccgca ctattgatgt acacattcgc aaggttcgtg aaaaaatcgg tatggaattt
     4801 atttcaacgg taaaaggtat tggttataaa tttgaaaatg aatgaaaaaa cactcgccta
     4861 agaatatttc attagagata gctttttatg tcatgctggt gttttttgtg ctgtttctgg
     4921 tgtattacct gatttttcag ggtcatcact cggattggtt gatcatactg attatcggcc
     4981 cattgttttt cggagccgtt tatcttatca tcaggcattc tgttgaaaat tttatttatg
     5041 aaaagatcag ggttttgtat aaaactctgc ataacctgaa aacaccgaaa caaaaaacaa
     5101 aaattgaaga acactggttt gaaaatgcag aagcggaaat agaaaagtgg gtggaagata
     5161 aaaaaaacga gctggaaagc ctgcggaagt ccgaaaacta ccgccgtgat tttctcggta
     5221 atgtttcgca cgaactaaag actcccattt tcaatattca gggatatata cttaccctga
     5281 ttgatggagg ccttgatgat aagagcatca accgcgagta cctgttgcgt gcagaaaaaa
     5341 gtatcgacag gatgattgct attgtcaacg atctggatgt tattgcccaa cttgagtctg
     5401 aaaccatgaa aatggaaatg gaaaaattcg accttacgca gctggcgcac gatgtttttg
     5461 aaacctatga aattaaagga aagcagaaga atatttcatt tattcttcac aataaactct
     5521 ccggcattgt tcatgttatg gcggacaagg aaaaaatcag acaagtattg aacaacctta
     5581 ttgaaaacgc catcaaatat ggcaaacaag atggccgtgt caaggtaact ttttacgata
     5641 tggacgaaat ggttctggtg gaagtatcgg ataacggcat aggtatttca caggaagata
     5701 tcccaagaat ttttgaacgt ttttaccgca ccacacaagg caaaagcctt gaaaacagag
     5761 gcagcggcct gggcctggcc attgtgaaac acatcatcga ggcccacgga caaaccatca
     5821 atgtccgcag tatgctgggt gcaggcacta cctttgggtt tacgctgcaa aaagcataat
     5881 agaggattat ttgttgcttc aaaagaaatc aatgcttgaa aaacaatacg tcaacaagcc
     5941 tttcgatcat acaagcacct aattatacga ttataaaaag aaatatttcc gcctttagcg
     6001 acatagaatt ataattgatt gtcagaagaa atcctcaata ttcaaataaa taataacttt
     6061 gtttgagtga atatcaatac gaaaaaccca tacaatacaa tgaaaaatgc acgatataac
     6121 aacaaaatca taaaaatcag gcaacgtatt tacgagataa tttttgaggc cgacaccaaa
     6181 gccggcaaac ttttcgactt aggtattttg actatcatac tcctgagtat atttttggta
     6241 atcctcgaaa gcatcagcag catccgcatg caatacggcc atatattgag gatattggaa
     6301 tggggtatta ccgtagtttt tacactcgaa tacctagcca gaatttgggt tactgaaaaa
     6361 ccattgaagt acatcttcag catttacggc attattgatt tactcgccct gttgcctact
     6421 tatctcgatg ttttctttgt tggcggacac aacatgattg tgatacgcgc actgcggctg
     6481 ttacggatat tcaggatatt taaactgcac cgttatacaa aagcaggccg taccattgcc
     6541 tcggcacttt ggagcaaccg cgaaaaaatc agtattttta ttttatttgt ttttaccatt
     6601 gccattatca ttggcacagt gatgtacctg atagaaggcg aacaaaacgg atttaccgac
     6661 atccccacct ccatctattg ggccatagtt accctcacca ccgtcggata tggcgacttg
     6721 tcgcccagta ccggaatagg ccaatttttg gctagtgtag tcatggtgct cggctattca
     6781 ataattgctg ttcccaccgg catggtcacg gccaccctta tcggacaaaa gaaggtaacc
     6841 aacactcagg tttgttcccg gtgcatgtac gacaagcacg acgacgatgc caattattgc
     6901 aaaatttgcg gcaccagtct ccatccaaat tcagaaagca ggaaaaacaa taccactacg
     6961 gatttggatt aaaaaaaatg tgaaaagaga aaacaggatc ggatgctaac aaatcaatac
     7021 ttcagccatt tccacagctc ccggtaactg acctttttgc catacattaa tattccggta
     7081 cggtaaattt tggcagcaag ccaggtgcaa gccagaaagc caagaatcaa caacgccatc
     7141 gaaagtataa actcccacgc aggcactcca aaaggaatcc tgatcatcat aacaatcgga
     7201 gacgtgagcg gaattaccga aaaccaaaat gctaccggcc cctgcggatt ttcaatgatt
     7261 ggctgcgcta aaacaaaggc cagtatcagc gggatagtaa taggcaacat aaactgctgc
     7321 gtgtcggctt cgttgtcaac cgcggaaccg atggccgcaa acagggccgc atacaacagg
     7381 tatccaaaca aaaagaagaa aaagaatgaa aaaattataa gcgaataatt gtattcgcca
     7441 aagcttttta ccagcgaccg gacactttcg tcgtcgtaat cctgaacagg tgcaggcgta
     7501 ttgttttcgg gcaccagcac attaccctgc tgcacggttt cggtttgcgg tttcgacaag
     7561 atatccttcg cccctaccga cacctgaaat accgccacca gcgacaaggt aagcacaatc
     7621 cataacaaaa actgtgtcag gccaaccagg gctatgccga taatttttcc caacaacaat
     7681 tggaatggct tgactgagga cacgatgatt tcaacaatcc ggcttgtttt ttcttctata
     7741 acgccccgca ttacctggtt tccatacata aagatggcaa aatatatcaa aaatcccgca
     7801 aaaatgccca tgattgtcgt agctatcggc aggttggtct ccccggtttc gatgttttgc
     7861 gaattgatgt tgatgcgtac tttggcttca tcaatttgct ttttatctat gccgtataat
     7921 tccatcagct tcctgttctc atagatatat ttcattttat ctttgatata cgaagtaacc
     7981 gtattgcccg gttgtttgtc cgaataatac aatatggcgt tggtaggttc gagcatatcc
     8041 ggcttgggaa tatacaacaa ggctgcggat ttgtcgccgt caaagccggc gatagcagtt
     8101 tgcaggtcgg tataaatata gttgaatttg atggtttcgt tactgcccat cttatagtag
     8161 attccggttt cgtcgagaac atcgataacc tgctgctccc tgtcgagttt ggtaacatag
     8221 tatggaacta tcatcagggc ggccatcagc aacgggccga ggatagtcat tacaataaag
     8281 gattttttct ttaccctcga cagatattct ctttttatga tgatgaaaat tttgttcata
     8341 agcattattc tgttaggccg gacgatgaat agttgagatt aatatcagca tttactttag
     8401 cgatgaaaat atcgttcatc gtgggtacta tttcgtgcag ggcgtggata gtgccatggg
     8461 gcatcagggc cgatagcata tcattggttg tcttgttgtt caatatttta attttcgctt
     8521 tcagcagccc atcctgcagc acttcgtcat aaacctcaaa atccttgttc agcctttctt
     8581 ttaacgaagc gcccgtgcct gcaatctcca cctgatagct aaacgacctg taggaatatt
     8641 taatttcatt aaccttgcct tccagaattt tgtttgattt attaatcagg gcaatatcat
     8701 cacacaactc ctccaccgag gccatattat gtgtggaaaa caccaccgtg gcgccattat
     8761 cacgtagacg aagaatctcg tctttgatca ggctggcatt aatagggtca aaaccgctga
     8821 atggctcgtc aaatatcagc agtttgggtt cgtgcaacac ggtaacgata aattgtatct
     8881 tctgttgcat tccttttgag agttcttcca ccttacggtt ccaccaactc atcaattcaa
     8941 atttctcaaa ccagtatcta agttttctta tcgcatcgct gtatgtcaag cccctgagct
     9001 gtgccagata aatagcttgt tcgcccacct tcattttttt atacagacca cgctcttcgg
     9061 gcaggtagcc gatatttact atatggtttt tttccagctt ttcgcccttg agaaacactt
     9121 ctccctcatc aggtccggta atcatgttga tgatgcggat aagcgtagtc tttccggcgc
     9181 cgttaggtcc aagcaagcca aaaatacttt gttcaggtac gctgatggat acgccattca
     9241 aggccacatg attggcaaaa cgcttggtta tattacgggt ttcaagcacg ttcattttta
     9301 tcgtatatca aaatatttac aactacaacc gttaactatt gacttaaatc cgtttattga
     9361 aagaattctt tcatcttctc aaaaaagttc ttatcctttt tgtctggctc gggtttgaaa
     9421 ttttccagat
//
LOCUS       DAOSFP010000037         9373 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6247_length_9373_cov_3.976604, whole genome shotgun sequence.
ACCESSION   DAOSFP010000037 DAOSFP010000000
VERSION     DAOSFP010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6247_length_9373_cov_3.976604"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            356..697
                     /gene="hypA"
                     /locus_tag="PLI16_01990"
     CDS             356..697
                     /gene="hypA"
                     /locus_tag="PLI16_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOH83360.1"
                     /translation="MHELSIATSIIEIAEDFARSHQAEKISRIEIEVGQLSGIVKESL
                     VFALELAVKNTVLESAEVVITEIPGKSICQACKHEFDNPDWYTPCPECQSMNTEITAG
                     KELQIKSIFTG"
     gene            733..1638
                     /gene="hypB"
                     /locus_tag="PLI16_01995"
     CDS             733..1638
                     /gene="hypB"
                     /locus_tag="PLI16_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOH83361.1"
                     /translation="MCDTCGCSDPNAGVSYHKPGEEHTHQAPHNHTHEHHYEHDHEHE
                     HGQEHHSHTHHHAYAHEHAHDAHTHNHDHSHEHTGHQHEHDHTHEKEYKIEHDILDKN
                     KMLAERNRGYFDAKKIKAINLVSSPGSGKTTLLEKTISQLKQELLLYVIEGDQQTMND
                     ANRIHATGVPVIQINTGSGCHLDAEMVSNSVKQLDVADNSILMIENVGNLVCPALFDL
                     GESHRVVIISVTEGEDKPLKYPHMFHSSDVCIINKTDLLPYVDFSVEKAKEYALQVNH
                     HLQFFEISAKTGEGMDTWLGWLKKL"
     gene            1721..2122
                     /gene="nikR"
                     /locus_tag="PLI16_02000"
     CDS             1721..2122
                     /gene="nikR"
                     /locus_tag="PLI16_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOH83362.1"
                     /translation="MKVKRFGVSLDESILAELDHFVNLRKFPNRSQAIRYLISNYLVE
                     EKADENKIVAGSIVLVYDHHKRELQSRSTSIQHDYHETILSVMHVHINHHNCLETIAV
                     MGKASKLKELADKLIAIKGVKHGKLVITATS"
     gene            2175..3050
                     /locus_tag="PLI16_02005"
     CDS             2175..3050
                     /locus_tag="PLI16_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83363.1"
                     /translation="MKLINNSILFLLALILCSPIQIKAHEEPAKTLKEKNQKEAERKK
                     IKDSKIKSLVLYTIIYNDKQQIEKKEKTFVMKYNEHGNFISVEAYKNDSLNLTVSYEY
                     DGNDNIISDIDYSPQGLPLEKNLYTFDMQGRVVSGKSFIKEDSLEGRFVIEKSNNKKK
                     LDFIKYKADNSTDYKITYAYDDDFDKTDYTEAVKYNSDGTINIKVTKKYNDNFRQTEK
                     AVYDGSGNKLYFFTYEYDKSGNNVKISKSKPDGTIDWSDNYSFDKHGNCTGMKSFDSK
                     NMPVTELIYDYEYFK"
     gene            3067..3495
                     /locus_tag="PLI16_02010"
     CDS             3067..3495
                     /locus_tag="PLI16_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH83364.1"
                     /translation="MRICQKIFYVVFALFAMNNISSIAVAQEGDSTKIPLTPKAITFN
                     IELDEYQELLNGRPSSDGIFSGLVTLKPGEKVGEHDTEHYEEVLVVLSGEGQMIFKNG
                     SLLELKFGVLAYCPPNTAHNVKNTGKKPLKYLYIATDTEK"
     gene            3746..4447
                     /locus_tag="PLI16_02015"
     CDS             3746..4447
                     /locus_tag="PLI16_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOH83365.1"
                     /translation="MAQLPFITDNSGTVGQWTKQIEMSYGIGFDNGHRCTKSVAEISP
                     VLTVGVHDKVDIVLGYPYLFLSEVDDSVISRYSGFSDLCIEVKYRFLDYKFLSLAIKP
                     GISFPTGNYKIGLGNGRFGYSAFLITTLSFNKIFVHSNFGYIRNENRCGDAKDIWHAS
                     IGLDYNIRNGVHAILNAGAEKNPDITSNIPSVFTILGLYCMLNEHVEISVGYKLGCTE
                     PETKHSFIAGLTFRF"
     gene            4464..5156
                     /gene="cbiM"
                     /locus_tag="PLI16_02020"
     CDS             4464..5156
                     /gene="cbiM"
                     /locus_tag="PLI16_02020"
                     /inference="COORDINATES: protein motif:HMM:NF008873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
                     /protein_id="HOH83366.1"
                     /translation="MHIPDGYLSPQTYVPLYGAFIAVTAVAVKKIRKEVSVKNIPYLG
                     MAAAFSFIIMMFNLPIPGGTTGHAVGSALVAIILGPWTAVIAVSVALIIQAFIFGDGG
                     ITAIGANCFNMAIFMPFAAYYLFKIFCRHGEKKSGVFWSSFVAGYISLFLAAVLTAVE
                     FGIQPMIAHSPDLKALYCPYDLKIAIPAMAIEHLALFCIAEGLITAMIVMYFYRHEKP
                     LIYALNKGGHHE"
     gene            5149..5484
                     /locus_tag="PLI16_02025"
     CDS             5149..5484
                     /locus_tag="PLI16_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HOH83367.1"
                     /translation="MNKLQKKILVFLIILTLLTPAGILLPIFFNAGDAWGEWSAETVK
                     DMIGYVPQGLAKYTDNYKAPLPDYSANSGDPSVIHQSGYYILSGVLGVIIILIVMFII
                     SKIIVKKNG"
     gene            5477..6403
                     /gene="cbiQ"
                     /locus_tag="PLI16_02030"
     CDS             5477..6403
                     /gene="cbiQ"
                     /locus_tag="PLI16_02030"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOH83368.1"
                     /translation="MDNSTPEFLLKPEVPVTNQTPGHKSGFGFIDRTLKATAKAMNTV
                     YYQANNSSKPTFTGKLHPATKLFSLIYIIVIISIVNSPAAQGIISGMLFLCFVFSSFN
                     LVKTYKRLLVFGFFFGFIVVVPAAFNIITPGKTLWHLFSFPKEYRLWIYHVPQNIDVT
                     EEGALLVLRFFFRVFNSISITYLVVFSTPFAELIKSLKTLFIPDTFLMVIMLAYKYIL
                     ILSLSIEETYFAMRSRLLGHVRNKNIRILIAGRIHYIFRRSKQTYELTYLAMVSRGYT
                     GKIVLGRPQKIIYTDIIVLTIVVAFGITISFI"
     gene            6406..7218
                     /locus_tag="PLI16_02035"
     CDS             6406..7218
                     /locus_tag="PLI16_02035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH83369.1"
                     /translation="MEDFFLLDHVSYFYPGNVKAVDNISLNINKGGLFAIIGQNGSGK
                     STLLNIMNGLIYPASGKIFFDGSAICEKSLQNKDFNKNFRSRVSYIFQNPDIQLFCPT
                     VMDELLFGPLQMGIPYDMAIERAKQTLNFVGITELKDRQALMLSGGEKKKVAIASVLT
                     MNPDVILIDEPIAGLDPKTQTFFIELIIELNKNGKTIIFSTHHLDLIDHLQPTVAVLS
                     EDHTIQKTAAASEILNDEQFLISVNLIHEHVHKHGNEVHGHFHSHYVFHKHE"
     gene            complement(7233..7685)
                     /locus_tag="PLI16_02040"
     CDS             complement(7233..7685)
                     /locus_tag="PLI16_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOH83370.1"
                     /translation="MKKTLIYGYGNPGRQDDGLGARFVEMAEKWIAEERISQIFTDCN
                     YQLNIEDSAVIAEYDTVIFVDASVADVNKFKLEKVDPDNASIEFTMHAVSVSFVLDLC
                     QKVYHKSPETYVLHIKACEFDFIEALTPQAEENLLAAFEFLKRFIQQI"
     gene            complement(7746..9194)
                     /locus_tag="PLI16_02045"
     CDS             complement(7746..9194)
                     /locus_tag="PLI16_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOH83371.1"
                     /translation="MKKVTIEPVTRVEGHGKVTIHLDDNMNVAQTRLHIVEFRGFERF
                     VQGRPYWEAPVLVQRLCGICPVSHHLAAAKALDVIVGAGTGHGLTPTGEKMRRLMHYG
                     QMFQSHALHFFHLASPDFLLGYDADPAIRNVIGVAINFPELATQGVLMRKFGQEIIAV
                     TAGKKIHGTGAIPGGINKNLSIEERDALLKGADPLNIDKMIEWAYSAVQFFKDYYMKN
                     RDFVDNFAYFPSNHLSLVRKDGALDLYHGVIRAVDAEGNKILNDVDYQEYYKYIEEEV
                     RSWSYMKFPYMKHLGKEKGWYRVGPLARLNTCDFIPSPLAQKEFEIYKAHTQGKPNNY
                     CLHTHWARLIELLHSAEVIKDLLNDPDLQKNDLVTKGTKQKEGVGLIEAPRGTLFHHY
                     RINDDDQIVMANLIVSTTNNNEPMNQSVNFVAKKMMNGQKEITEPMKNAVEIAIRAYD
                     PCLSCATHALGKMPLDIKLFDSKGEELDHYRS"
BASE COUNT         2865 a         1881 c         1852 g         2775 t
ORIGIN      
        1 aatgaacaac ttaatttatt taatttcaat caaatattaa cgcatcagta atgatgttaa
       61 ttctttattc aaatgtttgt tgaaacttcg cggaagcgtc gtattagttc cggaagataa
      121 gcttaaaggg tttaattttt ttagaggcgg aataattaaa taaagattcc tcagttagct
      181 ttatttcctg cccttctgct tcattcaggc gggcatgtga atgagagatc aggaaggatg
      241 aaagcagctt tattagaaat ctcttacaat ttaccgcttt tacttttgtt ttttataata
      301 accgttaccg gaaaataaaa gctgaaagac agaaaaatct tatctttgct gccctatgca
      361 cgaactttcc attgcaacaa gtatcatcga aattgccgaa gattttgccc gcagccacca
      421 ggcagaaaaa atcagccgta tcgaaattga agtaggtcag ctttccggta tcgttaagga
      481 aagtttggtg tttgcccttg aattggcagt gaaaaatacg gtgctggaat ctgctgaagt
      541 ggtaattacg gaaatacccg gaaaatctat ctgccaggcc tgcaagcacg aatttgacaa
      601 tcccgactgg tacacgccat gcccggaatg ccaatcaatg aatacagaaa taaccgccgg
      661 taaagaatta caaataaaat ccatttttac aggctgattt tgtaactttg caaaaaacaa
      721 aaacttttta ttatgtgcga tacatgcggt tgcagtgacc caaatgcggg agtcagttac
      781 cataagcccg gtgaagaaca tacgcatcag gcgcctcata accatactca tgaacatcat
      841 tacgagcatg accatgagca cgaacacggc caggaacatc acagccatac tcatcatcat
      901 gcatacgccc acgagcatgc tcacgatgca catacccaca accatgacca cagccatgaa
      961 cataccggcc accagcatga acacgaccat actcacgaaa aagaatataa aatagaacac
     1021 gatattctgg acaaaaacaa aatgttggcc gaacgcaacc gtggttattt tgatgcaaaa
     1081 aaaataaaag ccatcaacct ggtaagctct ccgggtagcg gcaagactac cctgcttgaa
     1141 aagaccattt cgcaactgaa acaggaactt ttactatatg tcattgaagg cgaccagcaa
     1201 accatgaacg acgccaaccg tattcatgct accggcgttc cggtaataca gataaacacg
     1261 ggtagcggct gtcatctgga tgccgaaatg gtcagcaatt cggtaaaaca gcttgatgtg
     1321 gccgataact ctatcctgat gattgaaaat gttggcaacc tggtatgccc cgccctcttc
     1381 gaccttggcg aatcacaccg tgttgtcatt atcagtgtga ccgaaggtga agacaagccg
     1441 ctcaagtacc ctcacatgtt tcatagttcc gacgtctgta ttatcaacaa aactgatctg
     1501 ttgccttatg tagatttcag tgttgaaaaa gcaaaagaat acgccctgca ggtaaatcac
     1561 catttacaat tctttgaaat ctccgctaaa accggcgaag gcatggatac ctggctggga
     1621 tggctgaaaa aactttaaca aaaccttaat tttttatcat ttctttttgt tatcgaaaat
     1681 tattttattt ttgtgccaac tttttataaa aaagtaacac atgaaggtaa aacgttttgg
     1741 tgtatcgctt gatgaaagta tcctggctga actggatcat tttgtcaacc tgcggaagtt
     1801 tccgaaccgt tcgcaagcca tacgttatct gatttccaat taccttgttg aagaaaaagc
     1861 tgatgagaat aaaattgtgg cagggagtat tgttttggtt tacgatcatc ataaaaggga
     1921 attgcaaagc agatcaacca gtattcaaca tgattatcac gaaaccatcc tttcggtgat
     1981 gcacgtacac atcaaccacc ataattgcct tgaaaccatt gctgtgatgg gcaaggccag
     2041 caaattaaaa gaactggccg acaagctgat agctattaaa ggtgtgaaac acgggaagct
     2101 ggttataaca gctaccagtt aatttttttt atttctatgg taacactatt ttcaaaaaag
     2161 ataacacatt aatcatgaaa ctaataaata acagtattct ctttctcctg gccctgatac
     2221 tttgcagccc tattcaaatc aaagcccatg aagaacctgc caaaacactg aaagaaaaga
     2281 atcagaaaga agcggaaaga aaaaaaatca aagattccaa gataaaatcg cttgtcttat
     2341 ataccatcat atacaacgat aaacaacaga ttgaaaaaaa agaaaaaacg tttgtaatga
     2401 aatacaatga acacggcaat ttcatcagcg ttgaagctta taaaaatgac tcattaaatt
     2461 taactgtaag ttatgaatac gatggtaatg acaatataat ttccgatatt gattattccc
     2521 cgcagggttt gcccctggaa aaaaacttat acacttttga tatgcaaggg cgggttgttt
     2581 caggcaaatc ctttattaaa gaagattcac tggaaggccg ctttgtcatt gaaaaaagta
     2641 ataataaaaa aaagcttgat tttattaagt acaaagcaga taacagcacg gattataaaa
     2701 tcacatacgc ttatgatgat gattttgaca agaccgatta tacggaagca gttaaataca
     2761 atagtgacgg tacgataaat ataaaggtaa ccaaaaaata taatgataac ttccggcaaa
     2821 ctgaaaaagc tgtatatgat ggcagtggaa ataaattgta tttttttact tatgaatacg
     2881 acaaatcggg gaataatgta aaaatatcaa aatcaaagcc ggatggaacg attgattggt
     2941 cggataatta ttcgtttgat aaacatggaa actgtacggg catgaaaagt ttcgacagta
     3001 aaaatatgcc ggtaaccgaa ctcatatatg attatgaata ttttaagtaa attgaaaaaa
     3061 gcagatatgc gcatttgtca aaaaattttt tatgttgttt ttgcgctctt cgccatgaat
     3121 aacatatctt ccatagcggt agcccaggaa ggtgattcaa caaaaattcc gctcacaccc
     3181 aaagccataa catttaatat tgaattggat gaataccagg agctcctcaa cggcaggccg
     3241 tcatccgatg gaatattttc aggacttgtt accctgaagc cgggcgaaaa agtaggtgag
     3301 cacgataccg agcattacga ggaggtattg gttgttttga gcggagaagg tcagatgatt
     3361 tttaaaaacg gcagtttgct ggaattaaag tttggtgttt tggcttattg tccgcctaat
     3421 acggctcata atgttaaaaa tacagggaaa aagcccttaa aatatttata tattgctacg
     3481 gatacggaaa agtaattttt tattgaaaat aattatccta aagcactttt tcatattcac
     3541 atcacactac aaaagagaaa caaagaatta atcacataaa cataaatcgg gcaaactgaa
     3601 agaattagct gataaactta tcgccataaa agaaataaaa cacggcaaac tggtaatcac
     3661 aacaaaagaa taattttttt ttaaaatgag agtaacacgt tttttaatat tagtaacaat
     3721 tctagtactt ttttctatcc aaaacatggc acagttgcct tttatcacag ataacagtgg
     3781 gactgtcgga caatggacaa aacaaattga aatgtcttat ggtattggtt tcgacaacgg
     3841 acaccggtgc actaaatctg tggcagaaat ttccccggtg ctaaccgtgg gtgttcacga
     3901 taaagtggat attgtattag gttatccata tcttttttta tcggaagttg atgattcggt
     3961 tatttcaagg tattccggtt tctcggattt atgcatagaa gtaaagtatc gtttcctgga
     4021 ttataaattc ttatcactcg caattaaacc aggcatttca tttcccacag gcaattataa
     4081 aatcggactg ggtaatggcc gttttggata ttcagcgttt ctcattacaa cattgtcatt
     4141 taataaaata tttgttcatt caaattttgg ctacataaga aatgagaaca gatgtggcga
     4201 tgctaaggat atttggcacg cttctattgg gttggattat aatatcagaa atggggttca
     4261 tgctattttg aatgccggtg ccgaaaaaaa cccggacata acttcaaata taccctctgt
     4321 atttacaatt ttggggttat actgtatgtt gaatgaacat gtcgaaattt cagttggata
     4381 taagttaggt tgtactgaac ctgaaacaaa acactcattt attgcaggat tgacatttag
     4441 attttaaact taatcaaata attatgcaca tacccgatgg ttatttaagt ccgcagacct
     4501 acgtgcctct ttacggcgct tttattgctg ttacggcggt tgccgttaaa aaaatccgga
     4561 aagaagtttc ggtaaaaaac attccttacc ttggcatggc tgcagcattt tcattcatta
     4621 tcatgatgtt taacctgccc atcccgggcg gaaccacggg gcatgcggtg gggtcggcgc
     4681 ttgtggccat cattttagga ccctggactg ctgtgattgc tgtttcggtg gccctgatca
     4741 tccaggcctt tatattcggt gacggcggca ttaccgccat cggggcaaac tgtttcaaca
     4801 tggcgatctt catgcctttt gcggcatatt atctttttaa aatcttttgc cgccatggtg
     4861 aaaaaaaatc cggagtcttc tggtcatcgt ttgttgccgg ttatatcagc ctttttttag
     4921 cagcggtact aacagccgta gaatttggca ttcagcctat gatcgctcat tctcctgact
     4981 tgaaagcgct ctattgtccc tacgacctga aaattgcaat tccagctatg gctattgagc
     5041 acctggcact gttctgtatt gccgaaggtc tgatcacagc aatgattgtg atgtattttt
     5101 acaggcacga aaaaccattg atctatgcgc taaataaagg agggcaccat gaataagctt
     5161 caaaaaaaga ttttggtttt tcttattatt ttgacgttac tgacacctgc cggaatttta
     5221 ttgccgatat tctttaacgc cggtgatgcc tggggcgaat ggtcggccga aacagttaaa
     5281 gatatgatcg gctatgttcc acaaggattg gcaaaatata ccgacaacta taaagctcca
     5341 ttgccagatt attcagcaaa tagtggggat ccgtcggtaa tacatcaatc ggggtattat
     5401 attttatcag gtgtgttagg tgttataatc attctaattg tcatgtttat tatttccaaa
     5461 ataatcgtaa aaaagaatgg ataacagcac tcctgaattt ttgctaaagc cggaagtacc
     5521 cgtaacaaat caaacaccag ggcataaatc aggatttggt tttatcgacc gcacgctgaa
     5581 ggctacggca aaggctatga atacagtgta ttaccaggcc aataattcat caaagccaac
     5641 atttaccggt aagcttcacc ctgcaaccaa actattttca ttgatttata tcattgtcat
     5701 catcagcatt gtgaacagcc cggcagccca aggtattata agcggcatgc tgttcctgtg
     5761 ttttgtattt tcttcattca atcttgttaa aacatacaaa cggcttctgg tattcggctt
     5821 tttctttgga tttatcgtcg ttgttccggc agcattcaat attatcactc ctggaaaaac
     5881 actctggcat ttgttttctt ttcccaaaga ataccggttg tggatctatc atgtgcccca
     5941 aaacattgat gtaactgaag aaggggccct actggtcctg cgtttctttt tcagggtatt
     6001 caattcaata tccatcacct acctggtggt gttcagcacc ccgtttgccg agcttattaa
     6061 atcactgaag acactgttta ttcctgatac ttttctgatg gtcatcatgc tggcatacaa
     6121 atacattttg attttgagcc tgagtataga agaaacatat tttgcaatga gatcaaggct
     6181 gctgggacat gtccggaata agaacatccg catattgatt gccggacgaa ttcattacat
     6241 ttttcgccgg tcgaaacaga cttacgaatt gacttatctg gcgatggttt cgagaggata
     6301 taccggaaaa atagtccttg gccggccaca aaaaataata tacaccgata tcatcgtact
     6361 gactattgtt gttgcgtttg gaatcacaat ttcgtttata taactatgga agactttttt
     6421 ttacttgatc atgtaagcta tttttatccc gggaatgtca aagctgttga caatatatcc
     6481 ctgaatatta ataagggcgg gctctttgca atcatcgggc aaaacggtag cggaaaatcc
     6541 accttgttga atatcatgaa tggtttgata tacccggctt caggaaagat cttttttgac
     6601 gggtcagcga tatgcgagaa atcacttcag aataaagatt ttaataaaaa ttttcgcagc
     6661 cgtgtaagtt atatttttca gaatcctgac attcaattat tttgccccac tgtaatggat
     6721 gaacttttat ttggtccgct gcagatggga attccttacg acatggccat tgaacgagca
     6781 aaacaaacct tgaattttgt tggcatcact gaattaaagg accgtcaggc attaatgctt
     6841 tcaggcggag aaaagaaaaa agtagccata gcctcggtgc tcaccatgaa tcctgatgta
     6901 attttaattg acgaacctat tgccgggctt gatccgaaga cgcaaacatt ttttattgaa
     6961 ctgatcatag aattaaataa aaatgggaaa acaatcattt tcagcacgca ccaccttgac
     7021 ctgatcgacc acctgcaacc aacagtagca gtactttcag aagatcatac gattcaaaaa
     7081 accgcagctg cttccgaaat cctgaatgat gaacagtttc tgatcagtgt aaatctgata
     7141 cacgagcatg ttcacaagca tggtaatgaa gtacacggac actttcattc acattatgtt
     7201 tttcataagc atgaatgagg ctatgacggt cattagatct gctgtataaa tcttttcaaa
     7261 aactcaaaag ccgccaacag gttttcttca gcctgcgggg tcagcgcttc tatgaaatca
     7321 aactcacagg ctttgatatg caacacgtag gtttcggggc ttttgtgata tactttctga
     7381 cataaatcca gcacaaagga gacagacact gcatgcatgg taaattcaat acttgcatta
     7441 tccggatcaa ccttttcaag tttgaattta ttcacatcag ccacagatgc atcaacaaaa
     7501 attaccgtat cgtattcggc aatcactgcg gaatcctcaa tattcaactg ataattgcag
     7561 tcggtaaaaa tttgggatat cctctcctct gctatccatt tttcggccat ttcaacaaaa
     7621 cgcgcaccca ggccgtcatc ttgccttccg ggattgccat aaccgtaaat cagcgttttt
     7681 ttcatgataa gaaattaaaa aaaaagtccg ccggaaaaga cggacttttt gttattcaat
     7741 gcaaattaag atctgtaatg gtctaactct tcacctttcg agtcaaacaa tttgatatca
     7801 aggggcattt tacccagggc gtgagtggca caactcaaac agggatcgta cgctctgatt
     7861 gcgatttcca cggcgttttt catgggttcg gtaatttctt tctgtccgtt catcatcttc
     7921 ttggccacaa aattcaccga ctggttcata ggctcgttgt tattggtggt tgatacgatg
     7981 aggttcgcca ttactatctg gtcgtcatcg ttgatccggt aatgatggaa caacgttccg
     8041 cgcggagctt caataagacc gacaccttct ttctgtttgg tccctttggt taccaggtca
     8101 tttttctgga ggtcgggatc gttcaacaaa tctttgataa cttcggccga atgcaggagt
     8161 tctatcaggc gtgcccagtg ggtgtgcaga caataattgt tgggttttcc ctgggtgtgg
     8221 gctttataaa tttcgaattc tttctgtgcc aaaggcgacg gaatgaaatc gcaggtattc
     8281 agacgagcca gaggcccaac acgataccaa cctttttctt ttcccagatg tttcatgtaa
     8341 ggaaacttca tataactcca ggagcgtact tcttcttcaa tgtatttgta gtattcctga
     8401 tagtcaacat cattgagaat tttgtttcct tcggcatcca ccgccctgat gacaccgtga
     8461 tacaaatcaa gagcgccgtc ttttctaacg aggctgagat ggttcgatgg gaagtatgcg
     8521 aaattgtcaa caaaatcgcg gtttttcatg taataatctt tgaaaaactg cactgcgcta
     8581 taagcccact ctatcatctt atcgatattc aatgggtcgg caccttttaa taatgcatcg
     8641 cgttcttcaa tgctgaggtt tttgttgata ccgccgggta tagcaccggt tccatgaatt
     8701 tttttaccag ctgtaacagc aattatttcc tggccgaatt tacgcataag aacaccctga
     8761 gtagccaatt cggggaaatt gatagcgaca ccaatcacgt tgcggatagc agggtcggca
     8821 tcgtatccaa gcaagaaatc gggagaagcc aggtggaaaa aatgcagtgc atgtgattgg
     8881 aacatctgcc catagtgcat cagccttctc atcttttcgc cggtcggggt aagtccgtgt
     8941 ccggttcctg cgccaacgat tacgtcgagt gctttagctg ctgccagatg atggcttaca
     9001 ggacagatac cgcataaacg ttgtaccaaa acaggcgctt cccaataagg acggccttgc
     9061 acgaaacgtt caaatccgcg gaattcaaca atatgaaggc gggtttgagc cacattcata
     9121 ttgtcatcga ggtgaatggt tactttgcca tgaccttcaa ctcgtgttac aggttctata
     9181 gttacttttt tcattatttt ctttattttt tatttaggtt tataattttg tcattcactt
     9241 caatgtcatt aattgtcatt cgcttcgctg tcattaattg tcattcgctt cgctgtcatt
     9301 aattgtcatt cgcttcgctg tcattaattg tcattcgctt cgctgtcatt aattgtcatt
     9361 cgcttcgctg tca
//
LOCUS       DAOSFP010000038         9298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6316_length_9298_cov_3.837390, whole genome shotgun sequence.
ACCESSION   DAOSFP010000038 DAOSFP010000000
VERSION     DAOSFP010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6316_length_9298_cov_3.837390"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2713
                     /locus_tag="PLI16_02050"
     CDS             <1..2713
                     /locus_tag="PLI16_02050"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH83372.1"
                     /translation="GLNKFDKKKGTFYHYLLDPNNANSLSNNNINCLLIDKKNNVWIG
                     TNEGLNYYNIQQDVFSHWYSGSRKNNALLNDLIRDLMLDNEGNLWICSDGGIDIYSPA
                     QQKIIKKYFHVGLKKNSLSDNRVRTVLQDNEGTIWVGTYGGGLNRYNKTTDDFFSYTN
                     DIYNSRSLSNNYINKIFQDKSGIIWVATAWKGVSKIDKYSNRFNHIQHLTETENSLNN
                     NNVWSIFQDQKGKVWIATDEGINIYDRESNKFSFITSKASDANSLPSNLVRHIYLDNN
                     NIFWFGTYDAGACSYNPQTGYYRQYAHDPKNPYSLSNNRINYIMKDSRGVLWVATDNG
                     LNQLNPLTYSFKRYLFDPENKNSISNNTVYSIHEDKQGILWVCTLDGLNRFNPLTGSF
                     SVLRKNPDNKHSLSTNRIFSIYEDEQGIFWIATFGGGLTRFNRATGEIKNYLEENGLP
                     NNVVYNIFEDKHNNLWLSTNYGLSRFNKAQETFVNYDVKDGIQSNEFNRNAAFYNTQT
                     GEMFFGGMNGFNSFFPENILTNNYIPPIVITDFKIFDKPQHLEIADGDTIYLSYEDNF
                     FSIEFAAMDYSNPAKNKYAYFLEKFDNDTIYCDASRRFASYTRVPPGSYVFRIKGSNS
                     DGVWNDQGLSFTIIIDPPWWRTWWFRIGYILLISLIAWYIIFLRLRNIRIRHEDEKRI
                     LEIEKQMFSLEQTALRLQMNPHFIFNSLNSIQSVVIANDTDKAINYLAKFANLMRFIL
                     SHSQKPFVNLSDEIRAMEIYLDVERLRFDNKFNYKIEIDPAIDEEFLEIPPMVLQPYI
                     ENAILHGLLNKEGVGNLTIQLIMKDKEYLKCVIEDNGVGREKAAEIRQRSGLQHKSKG
                     MMITQKRLEILNKSNKDHVNVKIIDLKDEKNQACGTRVELIIIYKEV"
     gene            2777..3523
                     /locus_tag="PLI16_02055"
     CDS             2777..3523
                     /locus_tag="PLI16_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOH83373.1"
                     /translation="MIRAIIIDDEKTSSDALMGLLKRYCENITVVAVADGYKSGVRAI
                     HEYRPDVVFLDIQMPDGSGFQVLEDVKEVNFEVIFTTAYDQYAIKAIKYSALDYLLKP
                     IIPSDLLNAIRKIEKKTEALGMTDNIKVLLDNLKNKSTSKKVVLSTAEKIHIVENDDI
                     IRCESDDYYTRFFFTDGKTLLISKTLKETEQLLGDTDFLRPHKSHLVNIKYIKGFLKN
                     DGGYIVLSDGSKVPVSRRKKEKVIQTIQNL"
     gene            3876..7529
                     /locus_tag="PLI16_02060"
     CDS             3876..7529
                     /locus_tag="PLI16_02060"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOH83374.1"
                     /translation="MLRKLLITAAVLITANMMAFSQSGTLKGKITDKGTNEPIPFANI
                     IIEQGGKQSGGTTSDFDGNFTIKPIPPGKYDVKATYVGYKPIMITNLVIVANQITFQD
                     VQMESTAINIETFEVVDYKVPLISKDQTSSGGTMTSEEISKMPGRSAQAVAITVGGVF
                     SQDGSMGSIRGARSDGTITYVDGVKVRGSSAVPKAAIEQVTVITGGTPAQYGDATGGI
                     LSISTKGASREFGMGFEGTTSQFLDKFGYNMLALNVQGPLIKGRDSTKTTALLGYFIA
                     AEYTYQKDPRPFETGNWKALDDVVERLEKNPLVPTGKGQGTYTAGEFLRNADLENIPY
                     KLNSANNDLNLSGKIDVRTSNTTNLTFGGTWQSNWGKEYVRDYSLLNWKKNPENTYNS
                     WRVYGRFTQRFNTDANSKATIKNVFYSIQGDYSKTTSITQDPEFKDDLFKYGYVGKMK
                     TYSTRSYEKTYIDSIGSEVYVHNGFADTLVTFEQTDINPILSNYTQNYYDLYAGEPDF
                     HYRNMDQIQLGGGLINGVFLGRDNVYGLWTNVGIPYNYYGRSEATQFGITANASADIG
                     NHAIQLGFYYEQRNDRYITYNPVGLWQSLRQQVNKHIIQLDLNNPHFAEDENHIFMDT
                     IWYDRLYSAGEQSLVDYNLRQKLGLQVDGTDWIDIDSYEPSTFTIDMFSADELLNGGN
                     AYVGYRGYDHTGKKTKGRPSFDDFFTKKDATGRFTRPIGAFEPIYIAGYIQDKFAFND
                     LIFNVGLRLDRYDANQKVLKDPYLLYEAKTVKEVTDLGPHPTNMGEDYVVYVDNVKSP
                     SSIKGYRSGSTWYNAAGTEISDPSAIETSSGIAPYLVNPDQKEVNSSAFTDYDPQLNF
                     MPRISFSFPISDEALFFAHYDVLTQRPSEGNVMNPTQYFFIYTQGQNAISNPNLKSEK
                     TIDYELGFQQKLNNSSSIKISAYYREQRNMIQVYRFSGAYPVSYISYNNIDFGTVQGF
                     TGTYDLRRTGNIWLKASYTLQFASGTGSNSESGLNLIRANQPNLRTITPLDFDRRHSI
                     SAVIDYRYGEGKEYNGPKITRKIKGTDKVKVIQLLKNTGLNVSINGGSGIPYSRSSTV
                     VGTQVGSGTYSLLGQINGSRMPWQFRVDARLDKDIIIKTGKKGEKTGRPLFMNIYLEV
                     LNVLNSKNVLSVYRATGNPGDDGYLTSADFQAQILAQTDSQAYIDQYSIKVNNPYYYS
                     LPRRIRLGLSLAF"
     gene            7565..>9298
                     /locus_tag="PLI16_02065"
     CDS             7565..>9298
                     /locus_tag="PLI16_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83375.1"
                     /translation="MKNIFRYLFLTLFIIGFSKTNYADKWPVLKSQKSETSTNAEACL
                     PASASTDLNVNNVRARINTGGDMWWDLQSNPEYFIPGNTTKTAMFSAALWIGGLDVNG
                     QLKLAAQRYRGNGNDFWTGPLTKDGTASVEAEVCDYYDKHFVITRAEVEEFIAACASN
                     GGTPPSSYTIPECIKTYPAHPYFTSSVHSHFLAPFMDVDGDLEYVPENGDYPYYDFDN
                     ALCPINYPCGYQAPKTMEGNGIMADQVLKGDMTLWWVFNDKGNIHTETKGQAVGMEIR
                     AQAFGFSTNDEINNMTFYTYEIINRSTYRLTETYFSQWVDTDLGDATDDYIGCDVLRG
                     LGYCYNGDDIDGTGRPQDYGMQPPAVGVDFFQGPYIDPDGLDNPAYDINNPLVNCNNA
                     INGVNFGNCIVDDERFGMRRFVYHNNTGGLDAITDPDVAPGYYNFLRGIWKDNERMSY
                     WGNGHPSAGGTGPACDFMFPGDTDPCLWGTGGVAPGAPYPTNYWTEQQAGNLPYDRRF
                     MQSAGPFTLEPGAVNYITVGIPWARATSGGAWASVELLRTVDDKCQALFDNCFKVLDG
                     PDSPDLTVQELD"
BASE COUNT         2870 a         2015 c         1916 g         2497 t
ORIGIN      
        1 cggccttaac aagtttgaca aaaaaaaggg gacattctac cattatttgc tcgatccaaa
       61 taatgcaaac agcttgagca ataacaatat aaattgtctg ctcattgata aaaaaaacaa
      121 tgtctggata ggcaccaacg aagggctgaa ttattataat attcaacagg atgtgttttc
      181 tcactggtat agtggctcac gtaaaaacaa tgccctgctg aacgatctta tcagggacct
      241 gatgctggac aatgaaggca atttgtggat ttgttccgat gggggaatcg acatatacag
      301 ccctgcgcaa caaaaaatca ttaaaaaata tttccatgtc ggattgaaaa aaaattccct
      361 cagcgataac cgcgtcagga ccgttttgca ggataacgaa ggaaccatct gggtgggcac
      421 ttatggtgga ggactgaacc gatataataa aactaccgat gattttttct cttatacaaa
      481 tgatatttac aatagcagaa gcctaagtaa caactatatc aataaaatct ttcaggacaa
      541 atcagggatt atatgggtgg ccactgcctg gaagggggta agtaaaattg acaagtattc
      601 aaaccggttt aatcatatac aacatttgac cgaaaccgaa aacagcctta ataataataa
      661 tgtatggtca atttttcagg accagaaggg taaggtgtgg atagccaccg atgaggggat
      721 aaatatctac gacagggagt ccaacaaatt tagttttatt accagcaaag cttccgatgc
      781 caactccctt ccgagcaacc tggtgcggca tatttatctt gataataaca atattttctg
      841 gttcggcacc tatgatgccg gtgcatgttc atacaatccc caaaccggat attacagaca
      901 gtatgctcac gatcccaaaa atccctatag cctgagcaac aaccgtatca attatatcat
      961 gaaagacagc cgtggcgtgt tatgggtcgc cacagataac ggcctcaacc agcttaatcc
     1021 cctcacgtat agttttaagc gctatctttt tgatccggaa aataaaaatt caatttcaaa
     1081 caatactgtt tatagtatcc acgaggataa acagggaatt ctgtgggtgt gcaccctgga
     1141 tggtttaaac cggttcaatc cgcttaccgg cagtttttct gttttgagga aaaaccccga
     1201 caacaaacat tctttaagca ccaaccgtat tttttccatt tatgaggatg agcagggcat
     1261 attctggata gctacctttg gcggaggatt aacccgtttc aacagggcta ccggagaaat
     1321 aaaaaattac ctggaggaaa acggcctgcc taataatgtg gtgtataaca tttttgaaga
     1381 taaacacaac aacctgtggc tttcaacaaa ttatggactt tccagattca ataaggcgca
     1441 agaaactttt gtaaattatg atgtaaagga tggcattcag agtaatgagt ttaatagaaa
     1501 tgcagccttt tacaacacac agaccggcga gatgtttttt ggcggaatga acggctttaa
     1561 ctcgtttttc ccggaaaata ttcttactaa caattatatc ccgccgatag taatcaccga
     1621 ctttaaaatt ttcgacaaac cacagcacct cgaaattgct gacggagaca ctatatatct
     1681 ttcgtatgag gacaatttct tttccattga atttgcggca atggattatt ctaatcctgc
     1741 aaaaaacaaa tatgcctatt ttcttgagaa attcgacaac gatacgatct attgcgacgc
     1801 ttcgcgccgt tttgcaagtt atacacgggt gcctccgggc agctatgtat tcaggataaa
     1861 gggttcaaac agcgacggcg tatggaatga tcaagggttg tcctttacca tcattattga
     1921 ccctccctgg tggcgcacct ggtggttccg tatcggttat attttgctga tctcgctgat
     1981 agcctggtat ataatttttc tgcgcctgcg caatatccgc atacgtcacg aagacgaaaa
     2041 acgcatcctg gaaatagaaa aacaaatgtt cagcctggaa caaacggcac tccgcctgca
     2101 aatgaacccg cattttattt tcaattccct caattcaatt caaagtgttg taatagccaa
     2161 tgataccgac aaggctataa attatttggc aaaatttgcc aatttgatgc ggttcatact
     2221 ttcacattcc caaaaacctt ttgttaacct gagcgatgaa atcagggcta tggaaattta
     2281 cctcgatgtg gaaaggcttc gctttgacaa taaatttaat tacaaaatag agatagaccc
     2341 tgcaattgat gaggaatttc tggaaatacc cccgatggtc cttcaaccct acatcgaaaa
     2401 cgccatttta cacggtttgc tgaacaaaga aggggtggga aaccttacaa ttcagttaat
     2461 aatgaaagat aaagaatacc tcaaatgtgt cattgaagat aacggcgtag gcagggaaaa
     2521 agctgcggag atacgacagc gttcgggact tcagcataaa tccaaaggca tgatgataac
     2581 ccagaaaagg cttgagatat taaacaaatc aaacaaagac catgtcaatg tgaaaatcat
     2641 tgacctcaaa gacgaaaaaa atcaggcctg cggaacccgg gttgagttaa ttattattta
     2701 taaagaagtg tagaaagttt gtatttttgt taatgataaa gaaaagatgt attaatattt
     2761 ttaagccttg taaaaaatga tacgagccat aattatcgac gatgaaaaaa cttccagcga
     2821 tgcgctgatg ggattgctta aaagatattg cgaaaacatt accgtggttg ctgtggctga
     2881 tgggtataaa tccggggtca gggctataca tgaataccgg ccggatgtgg tgtttctgga
     2941 tattcaaatg cctgacggaa gcggattcca ggtgctggaa gatgttaaag aggtcaactt
     3001 tgaggtgatc tttaccaccg cctatgacca gtatgccata aaagccatca aatacagcgc
     3061 attggattat ctgctcaaac ccatcatccc ttccgacctg cttaatgcca taaggaaaat
     3121 tgaaaaaaaa acggaagccc tggggatgac cgacaacatt aaggtgttgc tcgataacct
     3181 gaagaataaa agtacttcca aaaaagtggt gctgtcgacc gccgaaaaaa ttcatattgt
     3241 agaaaacgat gacataatcc gttgtgaatc cgacgattat tataccaggt ttttctttac
     3301 cgacggaaaa accctcttaa tctcaaaaac attaaaggaa acagagcaat tgcttggtga
     3361 tacggatttt ctcaggcccc ataaatcaca cctggtaaac attaaatata taaaaggatt
     3421 tttaaaaaac gacggaggct atatagtctt gtctgatggc tccaaagtgc ctgtatcgcg
     3481 aagaaaaaag gaaaaggtga tacaaaccat tcaaaatttg taataaaaag agttattttg
     3541 cccaccttct tataaaaaag gtttacgaaa actgtaattt tgtaccgata actttttttt
     3601 tccagccgtt aacttattca caaaataact tttattaata aataataaat ttgatacact
     3661 ttagtaaagt gtacttccat attgattttt taatgttagt gcgaaaaaaa acaagataaa
     3721 aaaaacgaaa aaatacctat tactaaaaac tttttggaga ccaaaatatt aatccctgaa
     3781 aaaaattatt tgcaatatta aagtgattat ttatatcttt accgaccttt ttggaggggt
     3841 ttatttgaca aaaaaaataa cataaactaa gaaatatgct tcgaaagtta cttattacag
     3901 cggctgtact catcacagca aacatgatgg cattctcgca atcaggaacc ttgaagggaa
     3961 aaatcacgga taagggcact aacgagccga ttccatttgc gaatatcatc atcgaacagg
     4021 gtggcaaaca atcaggtggt accacctcgg attttgacgg caattttacc attaagccca
     4081 tccccccggg aaaatacgac gtaaaagcga cctacgtggg atataagcca attatgatta
     4141 ctaacctggt gatagttgcc aatcagatta ccttccagga tgtgcagatg gaatctacgg
     4201 ccatcaacat tgaaactttt gaagtcgttg actataaagt tcctttgatt agcaaagacc
     4261 agacatcctc cggcggaacc atgacctcgg aagaaatctc caagatgccc ggacgttcgg
     4321 cacaggctgt tgccatcacc gtgggcggtg tgttctcgca ggacggatcc atgggaagca
     4381 tcaggggcgc ccgtagcgat ggtaccatta catacgttga cggtgtaaaa gtccgtggct
     4441 cttcagctgt acctaaggca gccatcgaac aggttaccgt tatcaccggc ggtactcccg
     4501 cacaatacgg cgatgctacc ggcggtatct tgtctatctc tacaaaagga gcctcgcgcg
     4561 agtttggtat gggattcgaa ggaaccactt cgcaatttct tgataaattc ggttataata
     4621 tgctcgcact taatgtgcag ggaccattga tcaaaggacg cgactccaca aaaaccacag
     4681 ccttgctcgg ctattttatt gctgctgaat atacctatca aaaagaccca cggccttttg
     4741 aaaccggtaa ctggaaagct ctggacgacg tagtcgaacg tctcgaaaaa aatcctctgg
     4801 tgcctaccgg aaaaggacag ggaacctata ccgccggcga atttttgcgc aatgccgacc
     4861 tggagaatat cccttataaa ttaaattcag caaacaacga cctcaacctt tccggcaaaa
     4921 tcgacgtccg tacttccaat actaccaacc tgacctttgg cggtacctgg caatcaaact
     4981 ggggaaaaga atatgtacgc gattattcat tgttgaactg gaagaaaaat cccgaaaaca
     5041 catataactc atggcgcgtt tacggccgtt ttacacagcg ctttaatacc gatgccaaca
     5101 gcaaggctac tataaaaaat gttttctatt ccatccaggg agactattca aaaactacaa
     5161 gcataaccca ggatcctgag tttaaagatg acctttttaa atatggttat gtgggaaaaa
     5221 tgaaaacata tagcacccgt tcttacgaaa aaacatacat cgacagtatc ggtagtgaag
     5281 tttatgtcca caacggattt gccgacaccc tggtaacctt cgaacaaaca gacattaatc
     5341 ccatcctgtc aaattatacg caaaattact atgatttata tgctggtgaa cctgattttc
     5401 attatagaaa catggaccag atccagctcg gaggcggcct gatcaacgga gtgttcctcg
     5461 gacgcgacaa tgtgtatggt ttgtggacca atgtcgggat accttataat tattatggac
     5521 gttccgaagc tacccagttt ggtattaccg ccaacgcctc ggctgacatc ggaaaccatg
     5581 ccattcaact gggattttat tatgaacaac gtaacgaccg ttatattact tacaaccccg
     5641 tgggattatg gcagtcatta cgtcagcagg tcaacaaaca catcattcag ttagacctta
     5701 acaacccgca ctttgccgaa gatgaaaacc atatttttat ggacactatc tggtacgacc
     5761 gcctgtattc agccggtgaa caatcattgg ttgactataa cctgcgccag aaactcggac
     5821 tgcaggttga tggcaccgac tggattgata tcgacagtta cgaaccttcc acctttacca
     5881 ttgatatgtt tagcgccgac gaactattga acggaggtaa tgcatatgtg ggttaccgcg
     5941 gatatgatca taccggtaaa aaaaccaaag gcagacccag ctttgatgac ttctttacga
     6001 aaaaagatgc caccggcagg tttacccgtc ccattggcgc ttttgaacct atttacattg
     6061 ccggttatat ccaggataag tttgctttta acgaccttat tttcaacgta ggacttcgtc
     6121 tcgaccgtta cgatgccaac caaaaggttc tgaaagaccc gtatttgctc tatgaagcaa
     6181 aaactgtaaa ggaagtaacc gatttaggtc ctcatcctac caatatgggt gaagattatg
     6241 ttgtgtatgt ggataacgta aaaagtcctt cctcaataaa aggttaccgt agtggaagca
     6301 cctggtacaa tgctgccggt accgaaatca gcgatccttc agctattgag acctcatcgg
     6361 gtattgcccc ttacctggta aaccccgatc agaaagaagt taactccagt gcttttaccg
     6421 attatgatcc ccagcttaat ttcatgcctc gtatctcttt ctcattccca atttctgacg
     6481 aagccctgtt ctttgctcac tacgatgtgc tgactcagcg tccctccgaa ggaaatgtga
     6541 tgaaccccac acaatatttc tttatataca cccagggaca aaatgctatc agcaatccta
     6601 acctgaaatc tgaaaaaaca attgactatg aattaggatt ccagcaaaaa cttaacaatt
     6661 cttcctctat taaaatttcg gcttattacc gcgaacaaag aaacatgatt caggtgtacc
     6721 gtttttcggg agcttaccct gtttcataca tttcttataa taatattgac tttggtactg
     6781 ttcagggttt taccggaacc tatgacctca gaagaaccgg aaacatctgg ttaaaagctt
     6841 cttatacttt gcagtttgct tccggaaccg gttcaaactc cgaatcaggg ttgaacctca
     6901 tccgcgccaa tcagccgaac ctgagaacca tcacccctct ggatttcgac cgtcgtcatt
     6961 ctatttctgc tgttattgac tatcgttacg gcgaaggaaa agaatataac ggaccgaaaa
     7021 tcaccagaaa aataaaaggt accgataaag taaaagtaat acaattgctg aaaaataccg
     7081 gactcaatgt ttctatcaat ggaggttcgg gtattcctta cagccgttcc agtacagtgg
     7141 ttggtaccca ggtaggatcg ggaacctatt cattattagg acagataaac ggttcgcgta
     7201 tgccctggca gttcagggtt gatgcccgtc tggataaaga catcattatt aagaccggca
     7261 aaaaaggaga gaaaaccggc agacctttat ttatgaatat ttatctggaa gtattaaacg
     7321 tactgaacag caaaaacgtg ctttctgtgt atcgtgccac aggtaacccc ggcgacgacg
     7381 gataccttac ctcagccgat ttccaggcac agatactcgc gcaaaccgat tcacaggctt
     7441 atatcgatca atattccatc aaggtaaaca atccttatta ttatagtttg ccccgcagaa
     7501 ttcgtctcgg actttcatta gctttttaac aaacaggatg acagaacatt ttaaaatcac
     7561 aaccatgaaa aatatttttc gttatttatt tttgacactt tttataatcg gattctcaaa
     7621 aactaactat gccgataagt ggccggtttt gaaatcccaa aaaagtgaaa caagcaccaa
     7681 tgcggaggcc tgccttcctg catctgcatc aaccgacctg aatgttaata atgtcagggc
     7741 ccgtattaat accggcggtg atatgtggtg ggatttgcaa agtaaccctg aatattttat
     7801 tccgggaaat actaccaaaa ctgctatgtt ctcggctgct ttgtggattg gaggtcttga
     7861 tgtcaatggg cagttaaaac tcgctgccca gcgctacagg ggaaacggaa acgatttctg
     7921 gaccggcccc ctcaccaaag acggtacagc ctcagtagaa gccgaagtgt gcgattatta
     7981 cgataagcat tttgtgatta cccgcgccga agtggaagaa tttattgcag catgcgccag
     8041 caatggtgga acaccgccct caagctatac cattcctgaa tgtattaaaa cgtatccggc
     8101 ccatccttat tttacttcaa gcgtacacag ccatttttta gctcctttta tggatgtgga
     8161 cggcgacctg gaatatgtcc ctgaaaatgg cgactatcct tactatgatt ttgacaatgc
     8221 gctctgcccg attaattatc cctgcggtta tcaggccccc aaaaccatgg aaggtaatgg
     8281 aatcatggcc gaccaggtgc tgaaaggtga tatgacactg tggtgggtat tcaacgacaa
     8341 aggaaatatt cacacggaaa caaaaggtca ggcagtaggt atggaaatca gggcacaggc
     8401 ttttggtttc tctaccaatg atgaaatcaa caacatgact ttttacacct acgaaattat
     8461 caaccgctcc acttatcgtt tgaccgaaac ttatttcagc cagtgggtgg atacagactt
     8521 gggtgatgct accgatgatt atatcggctg cgatgtatta cgcggactcg gttattgcta
     8581 caacggtgat gatatcgacg gtacaggccg tcctcaggat tatggtatgc agcctcccgc
     8641 cgttggtgtt gactttttcc agggacccta tatcgatcct gacggacttg acaatcctgc
     8701 ctatgacatt aacaatcccc tggtcaattg caacaatgca atcaacggtg tgaactttgg
     8761 aaactgtatt gttgatgacg aacgttttgg tatgcgtcgt tttgtttacc acaacaatac
     8821 cggcggttta gatgctatta ccgaccctga tgtggctccg ggatattaca attttttaag
     8881 aggtatctgg aaagacaacg aacgcatgag ttattggggt aacggacatc ccagtgccgg
     8941 cggcactggc cctgcatgcg acttcatgtt ccccggagat accgatccct gtttgtgggg
     9001 aaccggtggc gtggctcccg gagctcctta tcctactaat tactggactg aacaacaagc
     9061 cggcaacctt ccttacgacc gtcgttttat gcagtcagcc ggaccattta ctcttgaacc
     9121 tggcgctgta aattacatta ccgtgggtat tccctgggca agagctactt cgggtggtgc
     9181 atgggcttcg gtggaattgc tgcgcactgt ggacgacaaa tgccaggcct tgtttgataa
     9241 ttgctttaaa gtgcttgatg gtcctgattc acctgattta accgtacagg aactcgac
//
LOCUS       DAOSFP010000039         9295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6321_length_9295_cov_4.357576, whole genome shotgun sequence.
ACCESSION   DAOSFP010000039 DAOSFP010000000
VERSION     DAOSFP010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6321_length_9295_cov_4.357576"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="PLI16_02070"
     CDS             complement(<1..1859)
                     /locus_tag="PLI16_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH83376.1"
                     /translation="MKKNNQGIIESMMKHYSIVWLIVVLFVGFGIFGLINMNKQEFPE
                     FTLRQGVVAAVYPGATPEEVEQQLTKPLENFLFTYQEIDKKNTYSYSRNGIVYVFVEL
                     NKSVHNKDEVWSKIKHGLKDFKMQLPSGVLAIVVNDDFGNTSSLLITMESKDKNYREL
                     EKYMDQLCSKLRTVDAMGNIKVFGKQKEEIGVYIEQEKLASYGISSKTLMLNLYSQGF
                     LSMGGSLENDDIHIPIHINTPFTSEKEVAEQIVYTDPDGNVIRLKDIAKIEREYENPD
                     SYINKNGEEALVLSVEMQSGNNIVKFGKNVETVLQDFQKTLPASVSLYRITDLPKVVS
                     TSVSSFMRDLVFAILVVILVMLMLFPVRSALVAASGIPIAIAITWAFMFACGMELNTV
                     TLAALIVVLGMIVDNSIVVIDGYIDNLEHGYSRWHSAIKSAKDYFQPLLIATLAMSAA
                     FFPFMFTLTGPLHDFIKHFPWTFGFALTISLVIAMLMTPFLEYRFIKRPRANAKLSIA
                     IKAQNKFFTGLQGGYEWLLEKCFLHPVRTIAAGMLAIAISVVMFLFTPVQMMPVAERD
                     CFAVEIHLPAGSSLDQTQMVSDSMQKILMKDSRVESVTAFVGTSSPRFMATYAP"
     gene            complement(1912..2943)
                     /locus_tag="PLI16_02075"
     CDS             complement(1912..2943)
                     /locus_tag="PLI16_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH83377.1"
                     /translation="MMRKKALPAMLIMTLFVAGCGNQDKSLEKAAIPVKTLTMEQTTT
                     GADNNYVGTIVESFGTTLSFETAGNVEHVFVKEGQRVSKGQLLATLNESNAQNAYNVA
                     KATLEKARDGYTRAEQLYKNKSLSEIKWVEIQTALIQAQSLSDIAKKNLQDCKMIAPV
                     SGVVSSLDIEQGMNVIPFQSVIKVVDIEKLNVNVSIPENEIASVNIGQKATVTVSALG
                     NATYTGTITERGVEADVLSHCYDIKIELNNPEKKLLPGMVCKVQIESADTAQRAFHVP
                     VNTVQISNDGKRYVWVVMDGKAQRKFVTIGKLCNKNVIIESGIKEGDMVITEGWQKVS
                     EGSPVKCQQ"
     gene            complement(3027..3539)
                     /locus_tag="PLI16_02080"
     CDS             complement(3027..3539)
                     /locus_tag="PLI16_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83378.1"
                     /translation="MKTNSLKTCALLMLSCLFLSSCQKDGVKVFKGDYTYKTSGKITV
                     TISGVANPVKLLNRTGQMDIIDIGEDDRVMVVKTSLTGDVTSGYATIEGDKIIFDPVT
                     FNETLILLVENGLATITSNASGKIYENNTIIINETYEGIFAGQGSYLNVLGTISGDSI
                     LTVADRNEDD"
     gene            complement(3572..4012)
                     /locus_tag="PLI16_02085"
     CDS             complement(3572..4012)
                     /locus_tag="PLI16_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83379.1"
                     /translation="MAENQNNSTTNKPGNDAHYVLIADETGIAAAFNRLKKNLADHSF
                     GCITLVYAFLNDVSNPLYKAELQSLEKRFSNLLEVFIITHEAKASIMKAKIQKSIEVI
                     INSNINNVMQFYAGGNAEMVEQVKKILYFLGIKETQITTEYFNQ"
     gene            complement(4091..4654)
                     /locus_tag="PLI16_02090"
     CDS             complement(4091..4654)
                     /locus_tag="PLI16_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOH83380.1"
                     /translation="MKIPEYFKNIASLSAESERAIISAFKREELPKGHFLFQQEVICR
                     HIYFIEQGLARTYAISDSGREVTNWFFPENSFLTAINSFYNNSPSRDFCILTEDSVVY
                     SMTYSEFKRIVDDQPELAKFCFAVLFELAKQMTEYINSSKFQTAEDRYLELVKMHPAI
                     LQRASLGHIASFLGITQETLSRIRAKK"
     gene            5178..7004
                     /locus_tag="PLI16_02095"
     CDS             5178..7004
                     /locus_tag="PLI16_02095"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOH83381.1"
                     /translation="MKVFLSLVIIISIYYDVQCQTPDNFNKFMKDNGFVTYWPKMMCE
                     NVYRVDTKDKKSGLIDYPTLEIKVPAQYNFIKPIGTCTYYAGTLNSTGDTLTRKYFTI
                     DNQTLLPDDSKILSYKNKKFTIKTKTGKYAVYSNTGKQIIAPCDRLYDWDSRPDELQV
                     FMGAYALTFTDNNGALTDTNGTIVYRGIDSIECDYQHQVFIDQLAIVYKNGKAGIINQ
                     KGQVVLNFEYDQINRCIQIESFRFNGQPNGSLLAWLFAIKKGNKVGTADKTGKIIIPV
                     KYDDFALLTPYDEKYQAFDWNGNAWFRDGEKAGLFNINGQAITPLKYTDYKFFREKIA
                     PVAVKINDQVRWGYINTEGKEITEIKYIEADPCKNKLAVVQNESKMFGYIDMTGKEVV
                     PPQYVEARELAEGMGIVKSTSSLYGVVNQQGELVAPAIYRDIKDYDKGLAAVQDDKSG
                     LWGYIDKSGKVVADLIYKQISEYSDGVATVQDGKSGLWGYIDKSGKVVADLIYKQIIE
                     YDDGLAAVQDGKSGLWGYIDKSGKVVVDLIYKQIREYSIEEELAAVQHSKSEKWGFID
                     KSGKIVIKLKYDKVSDFYYGIAEVCEKGKCIYINRWGDEDED"
     gene            7129..7623
                     /locus_tag="PLI16_02100"
     CDS             7129..7623
                     /locus_tag="PLI16_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83382.1"
                     /translation="MNGKKQRGLKRYYKNLTVRNDFDKMNWLDFRQPGVWFDNWHLHF
                     DRKGYGNVSFKRRKPHLDKLFRHFDLLAEMTLNVKTDFQLFAVIHDFDSANDGIFLNT
                     PNPNSSVFPFSIPGLSLSNNLQNIHLADYLVKLSDYTILYGNAEENYCVLFKENIGVG
                     IISD"
     gene            7939..9198
                     /locus_tag="PLI16_02105"
     CDS             7939..9198
                     /locus_tag="PLI16_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH83383.1"
                     /translation="MKEILQTVIREQRQRATIKNEVERQVPAAYMRCREILVISGVRR
                     CGKSVLLHQIKKKQKERDYVLNFDDERLIDFKMEHFQELHEVFVELFGVQKTFYFDEI
                     QNIRGWEMFVRRLYDSGCKVFITGSNASMLSRELGTHLTGRYVQYELFPFSFGEFLQF
                     KRYPTLRTTGLTTVEKGKIMALFAEYQKKGGFPVFLSDDFDEYLKYLYESVLYRDVLV
                     RNKLVNEKEMLQMVNYLASSIARPFTHTSLAKVIGVKNVTTVKNYLGYVEDTYLLFQI
                     LKFDYSLKAQHINPKKVYFIDNALAMKLGFQSSPDQGRWLENLVFIELKRRRKDVFYH
                     WGATECDFVIREGYKITEAIQVCYSLSDEITKQRETAGLMDALNAYNLSEGLILTADQ
                     ESRLKKDGKNIRVMPVWKWLLQANKNL"
BASE COUNT         2743 a         1826 c         1952 g         2774 t
ORIGIN      
        1 ggcgcatagg ttgccataaa ccggggagaa ctggtgccga caaaagcggt taccgattca
       61 acacgggaat ctttcattaa tattttttgc atgctgtcgc tcaccatctg tgtctggtcc
      121 agcgaactgc cggcaggcag gtgaatttcg acggcaaagc aatcgcgttc cgccacaggc
      181 atcatctgca ccggtgtaaa cagaaacatg acaaccgata ttgcaatagc cagcataccg
      241 gcagctattg tcctcaccgg gtgcaaaaaa catttttcaa gcaaccattc ataaccgccc
      301 tgcaggccgg taaagaattt gttttgggct tttatggcta tggaaagttt tgcgttggcc
      361 cgtggccttt tgatgaaacg gtattcaagg aaaggcgtca tcagcatggc aatgaccagt
      421 gagatcgtaa gtgcaaagcc aaacgtccac ggaaagtgtt tgataaaatc atgcaaaggg
      481 ccggtaagtg tgaacataaa agggaagaaa gcggcactca tagccagtgt ggcgatgaga
      541 agcggctgga aataatcttt tgcgcttttt atagccgagt gccagcgcga atagccgtgt
      601 tccagattgt cgatataacc atcaataact acaatagaat tatctacaat cattccaaga
      661 acaacaatca atgccgccag ggttaccgtg ttcaattcca tgccgcaggc aaacataaat
      721 gcccaggtta tagcaatagc gatagggatg cctgatgcgg ccaccaatgc cgagcgcacg
      781 ggaaataaca tcaacatgac taaaattacc accagaatag caaataccag gtcgcgcatg
      841 aacgagctca cagacgtgct aaccacttta ggcaggtctg taatccggta caacgaaacg
      901 ctggcgggca gtgttttctg aaaatcctgc aacacggttt ccacattttt cccaaatttc
      961 acaatgttgt tgcccgactg catctccacc gacaacacca gggcttcttc cccattcttg
     1021 ttgatatagc tgtcggggtt ttcatattcc cgttctattt tagcgatatc tttcagccgg
     1081 attacgtttc cgtcgggatc ggtataaact atttgttcgg caacttcttt ttccgaagta
     1141 aagggcgtat tgatatggat cggaatatgt atatcgtcgt tttcgaggct gccgcccata
     1201 ctcaaaaagc cctgggaata caaattcagc atgagtgttt ttgagctgat tccataagaa
     1261 gccaattttt cctgttcaat atacaccccg atctcttctt tctgtttgcc aaacaccttg
     1321 atatttccca tggcatcaac cgtacgcaat ttactgcata actggtccat gtatttttcc
     1381 agttccctgt agttcttatc tttcgattcc atggtgatta ataatgaaga ggtatttcca
     1441 aaatcatcat tcaccactat ggccagcaca cccgaaggca actgcatctt aaaatccttc
     1501 agcccgtgtt ttatttttga ccaaacctcg tctttgttat gcacactttt gttcagttca
     1561 acaaaaacat aaacaattcc attacgggaa taggaatagg tattcttttt atcaatttcc
     1621 tggtaggtaa aaaggaaatt ttccagaggc ttggtaagtt gctgttcgac ttcttccggt
     1681 gtggccccgg gataaacagc tgcaacaacg ccctggcgta acgtgaattc cggaaactcc
     1741 tgtttgttca tattgatcag gccgaatatc ccaaaaccaa caaaaagcac tacgatcagc
     1801 cacacgatgc tgtagtgttt catcattgat tctatgatcc cttgattgtt ctttttcatt
     1861 ttagcacctc aaattttcaa aaccgcgtat atattatttc atatccggaa atcattgctg
     1921 gcatttcacc gggcttcctt cgctcacttt ctgccagcct tctgtaatga ccatgtctcc
     1981 ttcttttatt cccgattcaa tgatgacgtt tttattacac agcttcccga tcgtgacaaa
     2041 cttccgctgc gcttttccat ccatgacaac ccacacgtaa cgtttcccat cgtttgaaat
     2101 ctgtacggta tttaccggga catgaaaagc ccgttgcgcg gtgtcggcag attcaatctg
     2161 aactttgcat accatgcccg ggagcagttt cttttcggga ttattcaatt cgatttttat
     2221 atcgtaacaa tgcgaaagaa catcagcctc cacgccacgt tccgtgatgg ttccggtata
     2281 tgtagcattg cctaatgccg acacggttac tgttgccttt tgtccgatat tgaccgaggc
     2341 aatttcattt tcagggatac taacattgac atttagtttt tcaatgtcca caactttaat
     2401 tacagattga aaaggaataa cattcatacc ctgctcaatg tcgagcgagc tgaccacgcc
     2461 agaaacaggc gctatcatct tgcaatcctg taaattcttt ttagcgatat ccgacagcga
     2521 ttgtgcctga atgagagccg tttgaatttc tacccacttt atttccgaca gactcttgtt
     2581 tttgtagagt tgctccgcac gggtatagcc gtcccgggct ttttcaagcg ttgctttagc
     2641 cacattgtaa gcattttgcg cattgctttc atttaaagtt gccagcaact gtcccttgct
     2701 gactctttgg ccttccttaa caaagacatg ttccacattt ccggccgttt caaagctgag
     2761 ggtggtacca aacgactcga caatggtgcc cacataattg ttatctgcgc ctgtggttgt
     2821 ttgctccatc gtcaatgttt ttacagggat agcggccttt tccagcgact tatcctggtt
     2881 tccgcaccct gccacaaaga gcgtcattat gagcatagca ggtaaagctt tttttctcat
     2941 catgtttccc gttttttttt aataataatt cgcctgatga tttccccata aaatccataa
     3001 cgacaggggt tctaatcata catttttcaa tcatcctcat ttctgtcggc cacggtcagg
     3061 atgctgtctc cgcttatggt tcccaaaaca tttaaatacg acccttgtcc tgcaaaaatt
     3121 ccttcatagg tttcgttgat aatgattgta ttattctcat aaatttttcc gcttgcatta
     3181 ctggttatcg ttgccaaacc gttttcaaca agcaaaatca gggtttcgtt aaatgttacc
     3241 gggtcaaaga tgatcttgtc gccttcgata gtggcgtaac cactggtaac atcgccggtt
     3301 aatgatgttt taaccaccat caccctgtcg tcttcaccaa tgtcaataat atccatctgc
     3361 cctgtccggt taagcaattt gacgggattg gcaactccgc tgatggtaac ggttattttt
     3421 cctgaagttt tatatgtgta atcgccttta aatactttaa cgccatcttt ctggcaggac
     3481 gacaaaaaca ggcacgacaa catcagcagc gcgcatgttt ttaaactatt tgttttcatg
     3541 atttttaatt ttaacattca aaaggtattt tttattgatt gaaatattct gtggttattt
     3601 gtgtttcttt gatcccgaga aagtataata ttttctttac ctgctcgacc atttcggcat
     3661 tgccgccggc ataaaactgc atgacgttat tgatgttgct gtttatgatc acctcgatcg
     3721 atttttggat ctttgctttc atgatagaag cttttgcttc gtgagtaatg ataaacacct
     3781 ccaacaagtt ggaaaatcgt ttttcaagac tctgtaattc agccttatat aaaggattgg
     3841 acacatcatt gagaaaggca tagactaatg tgatgcagcc aaaagagtga tcggccaggt
     3901 tttttttaag ccggttaaaa gccgcggcta taccggtttc atccgcgatc agtacataat
     3961 gagcatcgtt tcccggtttg ttagttgtac tgttattttg attttcagcc attaatgcat
     4021 ctatttgggg gatgcaaaat tctgccaaaa caaaaccctg gcttttgaca aaaatcaaaa
     4081 aaaaggaaaa ctattttttg gctcttatcc tgctgagcgt ttcctgggta atgccgagaa
     4141 aagaggcaat atggcccagc gaagcacgct gtagtatcgc cgggtgcatt ttaacaagtt
     4201 ccaggtaacg gtcttcggca gtctggaatt tgctgctgtt aatatattcg gtcatttgtt
     4261 ttgccagctc aaacaaaacg gcaaaacaga attttgccag ttccggctga tcatccacta
     4321 tccgtttaaa ttcggaatac gtcatggaat atactaccga atcttcagta agaatgcaaa
     4381 aatcccgtga cggggagttg ttataaaaac tgtttatcgc ggtcagaaag gagttttcgg
     4441 ggaaaaacca gttggtaacc tcgcgcccgc tgtcggaaat agcataggta cgggcaaggc
     4501 cctgctcgat gaaataaatg tgacggcaaa taacttcctg ctgaaataaa aaatggcctt
     4561 ttggcaattc ttcccgcttg aaagcactga taatagcccg ttctgactca gcagaaaggc
     4621 tggcgatatt tttaaaatat tcggggattt tcattttggg gagatgtact ttttatgttt
     4681 ataaaattgg tggtaaaagt ataaatttat tattacttcg tttctatgta ttaccccgtc
     4741 atcgccctgt tttcgcatga ttgtatgatg tcgtaacagc gacatgtaca tcgaaattaa
     4801 taaaaaataa aaagacaggc cttgttttgt ttaaactctg cccttactgc atccgcctaa
     4861 gcaagaattc gggtggcaat aaggcacaaa taagctaaaa tcatttatca acaggcgcat
     4921 tctgtttaaa taaaatggtg taggcaaatc cgattacgca aaagaaagat tacaagattt
     4981 tgattgtttt atcaccgatt aggcaaaaat tttttctttg gtttgatttt tttacaacca
     5041 gaataaataa atagttaaaa taaattctat ttttatttat ttttgatgaa aattatataa
     5101 ccaaaaaatt tttaacaatt atgaatattt atttattttc ggcattttca aaaccagttt
     5161 taaccaaaca attatttgtg aaagtttttt tatcattagt gataataata agcatttatt
     5221 acgatgtaca atgccaaact cctgataatt ttaataagtt tatgaaagat aatgggtttg
     5281 taacttattg gcctaaaatg atgtgtgaaa atgtttatcg ggttgatacc aaagataaaa
     5341 aatccggact aattgattat cccactctgg aaataaaggt gccggcacag tataatttta
     5401 taaagccaat agggacatgt acttattatg ctgggacatt aaattcaaca ggcgataccc
     5461 tgacaagaaa atatttcact atagataatc aaacgttgtt gccggatgat tcaaaaatct
     5521 tatcatataa aaataaaaag tttaccataa aaaccaaaac aggtaaatat gccgtttact
     5581 caaacaccgg caaacaaatt attgccccct gtgaccgtct ttatgattgg gatagccgcc
     5641 cagacgagct tcaggtattt atgggtgctt atgcgttgac gtttacagac aataatggcg
     5701 cgctgacaga tacaaacggc actatagtgt accgcggaat agattccata gaatgtgact
     5761 accagcatca agtatttatt gaccaacttg ctattgttta caaaaacgga aaggcgggca
     5821 tcataaacca aaaaggccag gtagtattga attttgagta tgatcaaatt aatcgttgta
     5881 ttcaaataga atcctttcgg tttaatggcc aacccaacgg ttcattattg gcgtggttgt
     5941 ttgccataaa aaagggcaat aaggtgggca ctgcagataa aacaggaaaa attatcattc
     6001 cggtcaagta tgatgacttc gcgttattaa ctccctatga tgagaaatac caggcatttg
     6061 attggaatgg taatgcatgg ttcagagatg gcgaaaaagc tggccttttc aatattaatg
     6121 ggcaggcaat tacaccctta aaatacaccg attataaatt cttccgggag aagatagcgc
     6181 cggtggcggt taaaataaat gatcaagtgc gatggggtta tataaacaca gagggaaaag
     6241 agataactga aataaagtat atcgaagcag acccatgtaa aaataaactt gccgttgtgc
     6301 aaaatgaatc aaaaatgttt ggctacattg acatgacagg aaaagaggtt gtgccgccac
     6361 aatatgtgga agcccgcgag ctggcagagg gcatgggtat agttaaaagc acctcttctc
     6421 tttatggtgt ggtgaaccag caaggagaac ttgtagcccc ggcaatttac cgtgatatca
     6481 aggattacga taagggtttg gctgctgttc aggacgataa aagtgggttg tggggctata
     6541 ttgataaaag cggaaaggtg gtggctgatt taatttataa gcaaatcagt gaatacagcg
     6601 atggggtggc caccgttcag gatggtaaaa gcgggttgtg gggttatatt gataaaagcg
     6661 gaaaggtggt ggctgattta atttataagc aaatcattga atacgacgat gggttggccg
     6721 ctgttcagga tggtaaaagc gggctatggg gttatattga taaaagcgga aaggtggtag
     6781 ttgatttaat ttataagcaa atcagagaat attccataga ggaggagttg gcagctgttc
     6841 agcatagtaa aagcgaaaag tggggtttta ttgataaaag cggaaaaata gttattaaat
     6901 taaaatatga taaagtatca gatttctatt atggaattgc agaagtctgc gaaaaaggca
     6961 aatgtattta tataaataga tggggtgatg aagatgaaga ttaagaataa tgtacagaat
     7021 tgctattttg tggccaaata tttcaagata atattttata atgagcaata atacgtattt
     7081 tcatagtcat ctaatactga ttgtgctaca tattaagata ccggatgcat gaacggtaaa
     7141 aaacaacgcg ggctgaaacg atattataaa aatctgactg ttcgtaacga ttttgataaa
     7201 atgaactggc tcgattttcg ccagcccggt gtttggtttg ataactggca tttgcatttt
     7261 gaccgaaaag gatatggaaa tgtaagcttt aaacgccgga aaccccacct ggacaaacta
     7321 ttcaggcatt ttgacctgct ggcagaaatg accctaaatg taaaaactga ctttcaatta
     7381 tttgccgtta ttcatgactt tgacagcgca aatgacggaa tattcctgaa tacgccaaac
     7441 cctaacagca gcgtttttcc attttcgata ccaggcctgt ctctttcaaa taatttacag
     7501 aatattcatt tggctgatta tttggtaaaa ctaagcgatt atactatcct gtatggaaat
     7561 gcagaagaaa attattgtgt tttgtttaaa gagaatatcg gtgttggcat aatctcagat
     7621 taaacgacag aaggatgcaa acctgtctga atggctcagc cagactttag tcagatttaa
     7681 actttttaat gatagtcttt gcgcagaccc tggctactcc aggcactgtc ttacttctgg
     7741 ttcagcagct tctttatctt attcagccat atcacctcct ccccggggta tgagtgtgtt
     7801 gatgttgatg ttggtgttca acacgacatt tattaccagc atatttatta attattctat
     7861 tctcaatgga ataaaactca tggaaaactc tatttgtaat agaataattt gtatctttgt
     7921 gaaagcaaaa agtttactat gaaagaaata cttcagacag tcatcaggga acaacggcag
     7981 cgggccacca ttaaaaatga agttgaacgc caggtgccgg cggcttatat gcgatgccga
     8041 gagatactcg tgatatcggg tgtgagacgc tgcgggaagt cggtgctgct acatcagata
     8101 aagaaaaaac agaaagagcg ggattatgtg cttaactttg atgatgagcg cctgattgat
     8161 tttaagatgg aacattttca ggaattgcat gaggtttttg tagaattatt cggtgttcag
     8221 aaaacatttt atttcgatga gatacaaaac atccggggct gggaaatgtt tgtgcgccgg
     8281 ttgtacgaca gcggctgcaa agtttttatt accgggtcaa atgcatccat gctgagcagg
     8341 gagctgggta cgcatcttac cgggaggtac gttcagtatg agttgttccc gttttctttc
     8401 ggggagtttc tgcaattcaa aagataccca accctgagaa ctaccggatt gaccacagtc
     8461 gaaaaaggaa agatcatggc cttgtttgcc gaataccaga agaaaggcgg atttccggtt
     8521 tttttgtcgg acgattttga tgaatacctc aaatacctct acgagtcggt cttgtaccgc
     8581 gatgtgcttg tgcgtaacaa actggtaaac gaaaaagaaa tgctccagat ggtaaactat
     8641 cttgcaagca gcatagcgcg cccattcacg catacctcgc tggcaaaagt aatcggggtt
     8701 aaaaatgtaa ccaccgtaaa aaattatctg ggttatgtgg aagatacata tttgcttttt
     8761 cagatattga aattcgatta ctcacttaag gcacaacata tcaacccgaa gaaggtatat
     8821 tttattgata atgcgttagc tatgaagttg gggtttcagt cttctcccga tcagggccga
     8881 tggctagaaa accttgtatt tattgagctg aagcgtcgcc gtaaagatgt tttttaccat
     8941 tggggcgcca cagagtgcga ttttgttatc cgggaaggtt ataaaattac agaagccata
     9001 caggtttgtt attccttgtc ggatgaaatt actaaacagc gcgagacagc cgggcttatg
     9061 gacgccctga atgcgtataa tttatctgaa ggactgattt tgacagccga tcaggaaagc
     9121 agattaaaaa aggacgggaa aaacatccgc gttatgccgg tatggaagtg gctgcttcag
     9181 gcaaataaaa atttgtagtt atttcaggtt cagcaaacct tttagtgcta gtcagggtgc
     9241 gcatatgcgt caacttaaga aagagtccgt cataagaagc tgattaaaat ttgtt
//
LOCUS       DAOSFP010000040         9277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6335_length_9277_cov_3.410106, whole genome shotgun sequence.
ACCESSION   DAOSFP010000040 DAOSFP010000000
VERSION     DAOSFP010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6335_length_9277_cov_3.410106"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="PLI16_02110"
     CDS             complement(<1..115)
                     /locus_tag="PLI16_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOH83384.1"
                     /translation="MAILKPFKGLRPPKNIAHQVASRPYDVLNSKEARIEAD"
     gene            complement(186..629)
                     /locus_tag="PLI16_02115"
     CDS             complement(186..629)
                     /locus_tag="PLI16_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH83385.1"
                     /translation="MENEKNILFFRFEDLRIYNKAIDYVNWVYTHTQLVPESSRLCMT
                     NRFINAAQAISINISEGSSRNKTQFIYYLKMAKSAIRECIVYTTIAEKLQYLSTQEVE
                     ESRNALIEMTKMIGALIASLQRTEKPGEKTEDDFEDNLNINHDIA"
     gene            complement(677..1603)
                     /locus_tag="PLI16_02120"
     CDS             complement(677..1603)
                     /locus_tag="PLI16_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH83386.1"
                     /translation="MAKVLIATEKPFAAVAVNGIKKIFDEAGFETILLEKYTDKADLI
                     KAVADVDAMIIRSDKATAEVIDAAKNLKIIVRAGAGFDNIDLNAASARKIVAMNTPGQ
                     NSNAVAELALGMMVFISRQQFKGGSGNELKGKKLGMHAYGYVGKLVAAIAKGFGMEVY
                     AFDPFIEKSVIESEGVKAVDNVEELYSTCQYISLHIPANEKTKGSINFDLMSKMPKGA
                     TIINTARKEVICEESLKRMLNERPDFRYCSDIAPACHEEINGKYENRYYATPKKQGAE
                     TSEANINAGLAAANQIVGFLKNGITKFQVNKF"
     gene            complement(1731..2804)
                     /gene="serC"
                     /locus_tag="PLI16_02125"
     CDS             complement(1731..2804)
                     /gene="serC"
                     /locus_tag="PLI16_02125"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOH83387.1"
                     /translation="MKKVHNFNAGPSILPRVAIENTANAILDLNGIGMSILEISHRSK
                     DFQAIIDEAVALFKEQLGIPEGYHVLFLGGGASMQFCMVPYNLMEKKAAYLETGVWAK
                     KAIKEAKLFGEVLIVGSSADKNFSYIPKGWEIPKDVDYMHITTNNTIYGSEIRYDMDS
                     PVPLVADMSSDIFSRPVDVSKYALIYGGAQKNLGPAGATFVIVREDILGKVSRVIPSM
                     LDYRTHIKEGSMFNTPPCLPIYTIMETLRWLKSIGGVKVMEEMNKKKAAVLYDAIDSS
                     KIFVGTVEKDSRSLMNVCFVMKPDYQNLEEEFMNFAKSKGMIGIKGHRSVGGFRASIY
                     NAMPIESVQALVDCMKEFEASKA"
     gene            complement(2922..3899)
                     /locus_tag="PLI16_02130"
     CDS             complement(2922..3899)
                     /locus_tag="PLI16_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83388.1"
                     /translation="MAILVPAAVFSQDKSRQLDSLRLYSNTIASENPDFKKYEANEKL
                     QVLLEKIFSSEKYFEFPFDSLKKIAALTAPDKKFKLLTWGIAKENGTYEYFGYIVFPD
                     NQYGIKKYIKLNDKTVEVLYPETDITDHNKWFGAIYYDIIPCHYQGRKQYTLLGWKGN
                     TNLTTKKVVEILTFRSNGTPVFGKRLFRKNNKATRHIFEFSARTSMLLRYESQTLHTV
                     VKPAKTVKSSYSKKDKKSNKAISADKKVAAKVKTTRARMIIFDRLSPVDARTSKYVAN
                     LEGQYQFYVPETNIYDAFIFENGRWVFVKDVDARNPQPDKKKKFAPPQL"
     gene            4020..5093
                     /locus_tag="PLI16_02135"
     CDS             4020..5093
                     /locus_tag="PLI16_02135"
                     /inference="COORDINATES: protein motif:HMM:NF017686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HOH83389.1"
                     /translation="MDEKIIPITAEEHITNFKKLSEVLNCFVQRTTSGDLPETLILQE
                     AVDQSVKYNGFFTRESVLYSLTAIAGMLADGKLNQWAGAYPRLAGNIKTPKKIAVVMA
                     GNIPLVGFHDFLCVLISGNIFIGKLSQNDRFLLPAIARILIENDPRYRELICFTEDKL
                     QGFDAVIATGSNNTSRYFEYYFEKYPHIIRRHRNSLAVLSGNETPEQLHLLGGDIFNY
                     FGMGCRNVTGLFVPPNYDFAQFVSGMHSFAGINDHNKYRNNYDYYKTIFIINNQPFVD
                     GGFFILKEDSSINSPVAVIHFQQYNSLSEVRSFIDKNKDNIQCVVGGLFDGIHTIDFG
                     KAQSPELWDYADNVDTMEFLYNL"
     gene            5244..5558
                     /locus_tag="PLI16_02140"
     CDS             5244..5558
                     /locus_tag="PLI16_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOH83390.1"
                     /translation="MKKGIHPKDYRLVVFKDTSNDYTFITRSTAPTKETIVWEDGKEY
                     PLVKLEISHTSHPFYTGKMKFVDTAGRVDKFRNRYKKHYDKKGETRKEGLPENETPKS
                     EE"
     gene            5719..6957
                     /locus_tag="PLI16_02145"
     CDS             5719..6957
                     /locus_tag="PLI16_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HOH83391.1"
                     /translation="MNYILFDDSSRMDLYPMTLIRPVADIRIGIMTIREKWEKFLKDP
                     VSILTDKYLSTKYPLVKGKNNILINASVCPNSNLVKAIIRLKAGQALVCEDYIIAYNI
                     DENDLELGDTADEARIEDSDKIDLEGLNEIICDIPHMRISYPWDIFSFNGQAIKDDFA
                     LLTKGRKSAAISATNKVIAAENIFIEPGAKVECAIINATEGPVYIGKDAEVMEGAIIR
                     GPFALCEHSVLKMGAKIYGPTTIGPYSKVGGELNNTVIFGYTNKAHDGFIGNSVIGEW
                     CNLGADSNNSNLKNTYDEVKMWNYTQEKFIPSGLQFCGLIMGDHSKCGINTMFNTGTV
                     IGVNANIFGHGFQRCFIPSFSWGSSVSGYTTYDIAKALEVAKNVYQRRGLELSKEDMD
                     ILESVFKLTFPFRKKVKYVK"
     gene            7109..>9277
                     /gene="lon"
                     /locus_tag="PLI16_02150"
     CDS             7109..>9277
                     /gene="lon"
                     /locus_tag="PLI16_02150"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOH83392.1"
                     /translation="MEFSAELEKKLKLLSLVDNETEFIPLFTSEEEEQMNSEELPETL
                     PILPLRNTVLFPGVVIPITVGRDKSIRLIKEAYKARKNIGVVSQTDPNVEEPTFNDLY
                     KVGTVAIILKTLHMPDGSTTVIIQGKKRFTVLDIISTEPYFTARVGEYAQGEPARNDK
                     KFQALISSLKDFSIQIAKQSPNIPSEAAFAIKNIESPSFLINFVSSNLNIETAEKQLL
                     LETVNITERANIAMNHMVKELQMIELKNQIQNKVKTDLDKQQRDFLLQQQIKQIQQEL
                     GDNPQEQLISELKEKASGKKWPDEMAELFARELGKFQRMNPHAAEYSVQTNYLETIVD
                     LPWNEYTEGDFDLKNAQRILDEDHFGLEKVKERIIEYLAVLKVKGDMKSPILCLIGPP
                     GVGKTSLGKSIARALERKYVRMSLGGLRDESELRGHRKTYIGAMPGRIIQNLKKAKSS
                     NPVFVLDEIDKVSGNNVSGDPQAALLEILDPEQNTNFYDNFLEVDYDLSRVMFIATAN
                     SLSTIYPALRDRMEIIDLSGYLIEEKVEIAQRHLIPKQLREHGLQEKQILFGNKVLEK
                     IIDDYTRESGVRMLERTIAKVVRNRVRAIAMEEKYNKNIQQNELTKILGNPIYQKDKD
                     IPHDMAGVITGLAWTVVGGEILFIEVSLSKGKGNLTLTGNLGDVMKESATIAYEYLKA
                     HAPLLDIDSSVFENWNVHIHIPEGATPKDGPSAGITMVTSL"
BASE COUNT         2656 a         1917 c         2025 g         2679 t
ORIGIN      
        1 cgtcggcttc gatgcgggct tcttttgagt tcagcacatc ataaggcctt gaggccacct
       61 ggtgagcaat attttttgga gggcgcaacc ctttaaatgg ttttaaaata gccatgctaa
      121 taatttttga ttaattaata attgcgtact gtgataattg ctaaaacaac atttgcggat
      181 ggagcctagg cgatgtcgtg gtttatgttg aggttgtctt caaaatcatc ttcggttttc
      241 tcacccggct tttcggttct ctgcaacgat gctatcaggg cgcctatcat tttggtcatt
      301 tctatcagcg cattgcgcga ttcttccact tcctgtgttg acaggtattg taacttttct
      361 gcaatggtcg tataaacaat acattctctg atagcgcttt ttgccatttt cagataatag
      421 ataaactgtg ttttatttct gctggagcct tccgaaatgt tgatggatat ggcctgcgcg
      481 gcattgataa acctgttggt catgcacagc ctgctgcttt caggcaccag ttgcgtgtgg
      541 gtatagaccc agtttacata atcgatagct ttgttgtaaa ttctcagatc ttcgaacctg
      601 aagaaaagta tattcttttc gttttccatt cctttgagtt attatggtgc agtgttgtta
      661 atagcttaaa cccttattaa aacttgttta cctggaattt ggttatgcca tttttcagga
      721 aacccacaat ttggttggct gctgccaggc ctgcattgat gttggcttcg gatgtttcgg
      781 caccttgttt tttgggggta gcataataac ggttttcata ttttccgttg atttcttcat
      841 gacatgcagg ggcaatatcg gaacaatacc tgaagtccgg gcgttcgttg agcatacgtt
      901 ttaaagattc ttcgcagata acttctttgc gggcagtatt gataatagtt gcgccttttg
      961 gcattttcga catcaggtcg aaattgatgg atccttttgt tttttcgttt gcggggatat
     1021 gcagcgagat gtactggcag gtgctgtata attcttccac gttgtcaacg gctttcacgc
     1081 cttcgctttc gatgacggat ttttcaataa aggggtcaaa agcgtacact tccatgccaa
     1141 aacctttggc gatggcggct acaagtttcc caacatagcc gtaagcatgc ataccgagtt
     1201 tttttccttt taattcgttg cccgagccgc ctttaaattg ctggcgcgag ataaatacca
     1261 tcatacccag ggcgagttca gccacggcgt tggaattttg tcccggtgta ttcatggcaa
     1321 ctatcttacg ggcgctggcc gcattcaggt caatattatc aaatccggca ccggcacgga
     1381 caattatttt taggtttttt gcagcatcaa ttacttcggc ggtggcctta tcacttctga
     1441 taatcattgc atctacatcg gcaactgcct taatcaggtc ggccttgtcg gtatattttt
     1501 ccaacaagat ggtttcaaaa ccggcttcgt caaaaatttt tttaattccg tttacagcca
     1561 cagctgcaaa gggcttttca gtagctatta gaacttttgc catgataaaa ttagttttag
     1621 gtgtttaata taagtttttt agtaatgata aaacaaaaat agtaatttta aaacaaaaag
     1681 gataaaataa tctgcattaa caaattattt tatccttttg ttatttggaa ttatgccttg
     1741 gatgcttcaa attctttcat acaatcaaca agggcctgca cactttcgat aggcatggcg
     1801 ttgtatatag aagctctgaa accgccaacc gaacgatggc ccttaattcc tatcatgcct
     1861 tttgatttgg caaaattcat gaattcttct tcaaggttct gataatccgg tttcatcaca
     1921 aagcatacgt tcatcagtga acgcgaatct ttttctaccg tacccacaaa aatcttgctg
     1981 ctgtcgatag cgtcgtataa tacggcagct tttttcttgt tcatttcttc cattacttta
     2041 actccgccta ttgatttcag ccagcgcagt gtttccataa tggtatagat tggcagacac
     2101 ggaggggtgt tgaacataga accttctttg atatgggttc tgtagtcgag catcgaaggg
     2161 ataacccggg atactttgcc aaggatatct tcgcgaacaa tcacgaaggt agcgcctgca
     2221 gggccgaggt ttttctgtgc gccgccatag atcagggcgt atttggagac atccaccgga
     2281 cggctgaaga tatctgatga catatcggct accaaaggaa cagggctgtc catatcgtat
     2341 ctgatttccg atccgtaaat ggtattgtta gtggtgatgt gcatgtagtc cacatcttta
     2401 ggaatttccc atcctttggg gatgtatgag aaatttttat ctgcagaaga acctactatc
     2461 agcacttcgc caaaaagttt tgcttctttg atggcttttt ttgcccacac gcctgtttcc
     2521 aggtatgcag ctttcttttc catcaggttg tatggaacca tacagaactg catgctggcg
     2581 ccgcctccca ggaataatac atgatatcct tcgggaatgc caagctgttc tttgaacagg
     2641 gctactgctt cgtcgatgat ggcctgaaaa tctttgctcc tgtgcgaaat ttccagtatt
     2701 gacataccaa ttccgttgag atcaagaatg gcgtttgcag tgttttcaat ggctaccctg
     2761 ggcaggatag aaggaccagc gttaaaattg tgtacttttt tcatacttat ttaagtttaa
     2821 attattaagt tttttcatca tattttttgc aaagtaagta attataatgg aataaaaaaa
     2881 atgaaaagcc aggaaaaaat aactttttac aatacagtga tttacagttg cggaggagca
     2941 aactttttct ttttgtcagg ttgcggattg cgcgcatcga catcttttac aaagacccag
     3001 cggccgtttt caaaaataaa agcgtcgtaa atgttggtct caggcacata aaactggtat
     3061 tgtccttcca ggttggctac gtatttggag gtgcgggcat caacaggcga caggcggtcg
     3121 aaaattatca tccgggcccg ggtggttttt acttttgccg ccaccttttt atcggcagag
     3181 atcgctttgt tggatttttt atccttttta ctgtaggagg attttactgt ctttgcaggc
     3241 ttcaccactg tgtgcagggt ctggctttcg taacggagca gcatactggt gcgtgccgaa
     3301 aactcaaaaa tgtgacgcgt ggctttgttg tttttcctga acaggcgttt gccaaaaacc
     3361 ggtgttccgt tagaacggaa agtcagaatt tcaacaactt ttttggtggt taagttggtg
     3421 ttgcctttcc agcccagcag ggtgtattgc tttcggccct gataatggca ggggatgata
     3481 tcgtagtata tggccccgaa ccatttgttg tggtcggtta tatcagtttc agggtatagt
     3541 acttcaacgg ttttgtcgtt tagtttgatg tattttttaa tgccgtactg attgtcagga
     3601 aacacaatgt agccgaaata ctcataggtg ccattttctt tggctatgcc ccaggttaac
     3661 aatttaaact tcttgtcagg ggccgtcagt gcagcaattt ttttcagcga atcaaaagga
     3721 aattcaaagt atttttcaga cgaaaaaatc ttttccagca acacctgcag cttttcgttg
     3781 gcttcatatt ttttaaaatc gggattttcg ctggcaattg tattggagta taaccttaat
     3841 gaatccagtt gcctggactt gtcttgcgaa aaaactgccg ccggcaccag tattgccaaa
     3901 acaaaaaata ttaacaagcg ataacgcatc cgaattcgat aaatattaat gttaacgaaa
     3961 atcgtgtctg gtttatttta ccacgccaat ttccattatt tttgacaaaa atataaaaaa
     4021 tggatgaaaa aatcattcct attactgccg aagaacatat aacaaacttt aaaaagctgt
     4081 cggaagtgct gaattgtttc gttcagcgca ctaccagcgg tgatttacct gaaaccctga
     4141 ttttacagga ggccgttgac caaagtgtta aatacaatgg tttttttaca agggaaagtg
     4201 ttttgtactc gctgacggcc attgccggta tgctcgcaga tggcaaacta aaccagtggg
     4261 ccggagctta tccccggctt gccggcaaca taaaaacccc gaaaaagatt gcggtggtaa
     4321 tggccggaaa tattcctctt gtcggatttc atgatttttt gtgtgtgctg attagcggta
     4381 acatttttat tggaaaattg tcgcaaaacg accgtttttt attgccggcc atagcccgga
     4441 ttctgataga aaacgatccc agataccgcg aattaatttg ctttacagaa gataagctgc
     4501 agggattcga tgccgtgata gctacaggaa gtaataatac ctcgcgctac tttgaatatt
     4561 attttgaaaa atacccgcac atcatccgcc ggcaccgcaa cagccttgct gtgctgtcgg
     4621 gcaatgaaac cccggaacaa ctgcatttgc tgggaggcga catttttaac tatttcggca
     4681 tggggtgtcg caatgtcaca gggctttttg tgccgccaaa ttatgatttc gcgcaatttg
     4741 tgtcgggcat gcattccttc gccggaataa atgaccacaa caaatacaga aataattacg
     4801 attattataa aacgattttc atcataaata atcagccatt tgttgatgga ggatttttta
     4861 ttttgaagga agattcatca ataaattcgc cggttgccgt tattcatttc cagcaataca
     4921 acagcctgag tgaggtgagg tcttttatcg acaaaaacaa agacaatata cagtgtgtgg
     4981 tgggaggact ttttgacggg attcatacca tagattttgg aaaagcccaa agccccgaat
     5041 tgtgggatta tgccgacaat gtggatacta tggagttttt atataattta taaaaaaagt
     5101 atgttataac actgtaatac agaatgtaaa aatatcagtt taatataaat tttattaaaa
     5161 taaataaaaa aactttccga aactcttgtt tataaataaa agtagtgtaa ttttgcacct
     5221 gaattttaaa aaataaatca attatgaaaa aaggtattca tccgaaagat tatcgtttag
     5281 tggttttcaa agatacatcc aatgactata cttttataac ccgttcaacg gcgcccacca
     5341 aagaaactat tgtttgggaa gatggcaagg aatatccatt ggtaaaactt gaaatttcac
     5401 acacttcgca tccattttat acaggaaaga tgaaatttgt ggatacagca ggccgtgttg
     5461 acaaattccg caaccgttac aaaaaacatt acgacaaaaa aggagaaaca aggaaggaag
     5521 gccttccgga aaatgaaact ccaaaaagcg aagaataatg atttaaaaac cccgaaagca
     5581 atgccggggt tttttatttc ctttacagat agtatgttcg ctgggaaatc atgaaaattt
     5641 tcccctatta tttttatttt tttgtcataa atatcaggat attttgttaa tattgtagtt
     5701 tggcaaattt tactgcatat gaattatata ctttttgatg actcttcgcg catggatttg
     5761 taccccatga cgttaataag gcctgtggca gatatcagga tcggtataat gaccatacgc
     5821 gaaaaatggg aaaaatttct gaaggatccc gtgtccatac ttaccgataa gtatcttagc
     5881 acgaaatatc cgctggtaaa agggaaaaac aatattctta tcaacgcttc cgtatgcccg
     5941 aactctaacc tagttaaagc gattatcagg ctgaaggccg gtcaggctct tgtttgcgaa
     6001 gactatatca tcgcctataa tattgatgaa aatgacctgg aattgggcga taccgccgac
     6061 gaggcacgaa tcgaagacag cgacaagatc gacctggaag gactgaacga aattatctgc
     6121 gatatccccc acatgagaat aagctatccc tgggatatct tttcttttaa cggacaggcc
     6181 ataaaagatg attttgccct gctgaccaaa ggccgtaaat ctgcagctat cagtgctacc
     6241 aacaaggtaa tagctgcaga aaacatcttc atcgaacccg gagccaaagt ggaatgcgct
     6301 ataataaatg ctaccgaagg cccggtgtat atcggaaaag acgccgaagt aatggaggga
     6361 gctattatca gagggccttt tgccctttgc gaacattcag tactgaaaat gggagccaag
     6421 atttacgggc caacaaccat aggaccctat tcaaaagtgg gcggcgaact caacaatacg
     6481 gtgatattcg gatataccaa caaggcacac gacggtttta tcggtaattc cgtgattggc
     6541 gaatggtgca accttggcgc tgattccaat aattcaaacc tgaaaaatac ttacgatgaa
     6601 gtaaaaatgt ggaattatac gcaggaaaaa ttcattcctt caggcctgca gttttgcggt
     6661 ttgataatgg gcgatcattc caagtgcggg ataaacacca tgttcaatac cggcacggta
     6721 ataggcgtga atgccaatat tttcgggcat ggctttcaac gttgttttat cccgtcgttc
     6781 agttggggaa gctcggtgtc gggatatact acatacgaca ttgcaaaagc cctcgaggtg
     6841 gcaaaaaatg tttaccagcg ccgcggcctt gaattgtcga aggaggatat ggatatttta
     6901 gaatccgttt tcaaactgac ttttcccttc aggaaaaaag taaagtatgt gaagtaaatc
     6961 ggatgaatta aatgttttca tcaagcttgg cattttattt gacagagcct atgcctgatc
     7021 tgttgttttt gttggaacat acgcctttaa atcaaaaatt catatcagat ttgttgttga
     7081 actgacacat tgtcataatt ggaattatat ggaattttct gctgaattgg aaaaaaaact
     7141 gaagctatta agtcttgtcg ataatgaaac agagtttatc cctttgttta ccagcgagga
     7201 ggaagaacag atgaactctg aggagctgcc cgaaacactg ccgatcttgc ctttgcgaaa
     7261 tacggtattg tttcccggag tggtgattcc catcacggta ggacgcgata aatctatccg
     7321 gctgatcaaa gaagcctaca aagccagaaa aaacattggc gtggtttccc agaccgaccc
     7381 caatgtggaa gagcctacct ttaacgactt gtacaaagtc ggcacggtgg ctatcatttt
     7441 aaaaaccctg cacatgcccg atggcagcac taccgtcatc atacagggca agaaacgttt
     7501 tacggtgctg gatattattt ctacagagcc ttattttact gcacgcgtag gggaatatgc
     7561 acagggagaa cctgcccgga acgacaaaaa gtttcaggcg ctgatttcat ctctgaaaga
     7621 tttttccata cagatagcca agcaatcgcc caatattcct tcggaagccg cttttgctat
     7681 caaaaatatc gaaagcccgt cgtttctgat aaattttgtt tcttccaacc tgaatatcga
     7741 aacagccgaa aaacaactgt tgctcgaaac cgttaatatc accgaacgcg ccaacattgc
     7801 catgaaccac atggtgaaag agctgcagat gatcgaactg aaaaaccaga tacaaaacaa
     7861 agtaaaaaca gacctcgaca agcagcagcg tgattttttg ctgcagcagc agatcaaaca
     7921 gatacagcag gagctgggtg ataatcctca ggagcagctt atcagcgaac tgaaagaaaa
     7981 agcctcaggc aaaaagtggc ctgacgaaat ggccgaactt tttgcccgtg agttgggcaa
     8041 gtttcagcgt atgaaccccc atgccgccga atattcggtg cagaccaact acctggaaac
     8101 catagtggat ttgccctgga atgaatatac cgagggtgat ttcgatctta aaaatgccca
     8161 gcggatactc gacgaagacc atttcggcct cgaaaaagta aaagaacgca tcatcgaata
     8221 cctggcggta ctcaaagtta aaggcgatat gaagtcgccg attttgtgcc tgataggccc
     8281 tccgggagtg gggaagacct cactggggaa atccatcgca agagcgctgg aacgcaaata
     8341 tgtgcgcatg tcgctgggcg gcctgcgcga tgagtcggag ttgcgcggac accgcaaaac
     8401 atacattggc gccatgcccg gccgtatcat ccaaaacctg aaaaaagcca aatcttccaa
     8461 cccggttttt gtccttgatg agattgacaa agtgagcgga aacaatgtca gcggtgaccc
     8521 acaggccgcc ctgctcgaaa ttctcgaccc cgaacaaaat accaacttct atgataattt
     8581 tctggaagta gattacgacc tgtccagggt gatgttcatc gctacagcca acagcctttc
     8641 aactatttat cccgccctgc gcgaccgtat ggagattatt gacctgagcg gctacctgat
     8701 cgaagaaaaa gtagaaatag cccagcggca tctcataccc aaacagttga gagaacatgg
     8761 tttgcaggaa aagcagattc tttttggcaa caaggtgctc gaaaaaatta tcgatgatta
     8821 cacccgcgaa tctggtgtgc gtatgctgga acgtaccatt gccaaagttg tcagaaatag
     8881 ggtgcgcgcc attgccatgg aggaaaaata taacaaaaat attcagcaaa atgagctgac
     8941 gaaaattctg ggcaacccga tatatcagaa agataaggat attccccacg atatggcagg
     9001 ggtgatcacc ggcctggcct ggactgttgt cggcggcgag atattgttta tcgaagtgag
     9061 cctgagcaaa ggcaagggca accttaccct caccggcaac ctgggtgatg tgatgaaaga
     9121 gtcggctacc attgcttatg aatacctgaa agctcatgca cccctgctcg acatcgacag
     9181 ttctgttttt gaaaactgga acgtacatat ccatattcct gaaggagcca cgcccaaaga
     9241 cgggccatcg gctggcatca ccatggtcac ctcactg
//
LOCUS       DAOSFP010000041         9253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6357_length_9253_cov_3.751468, whole genome shotgun sequence.
ACCESSION   DAOSFP010000041 DAOSFP010000000
VERSION     DAOSFP010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6357_length_9253_cov_3.751468"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="PLI16_02155"
     CDS             complement(<1..1155)
                     /locus_tag="PLI16_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83393.1"
                     /translation="MKKQLLLVGMLCILTVFTMRAQDTIVAWTFPTGTLADSVANAGI
                     SSNIGTRTLMHEGGTGVTTMVPGDGTYAEEVAGWDNGDSTKFWSIKFKAEGYHDLVLY
                     SKQMSDGTLYGPRDWRVQYKWGSIPWTNVPGGTVVCNSAWNMGVVDSLPIPIAVPGGT
                     SIYIRWIMTSNIALDMNPVLPTGKSAIDNIIIMGTADLPVATGDTIIGFNFADTTDVE
                     FNADYGLANNLTYDIRAENDSAEVRTLTYTNGATDYAATAEGWDNGANFKFWSIKFKA
                     DDYYDMKVWSKQRSGQTYAGPKFWKLQYKLGGSGTWTDVPGGNITVANDWTTGVLNEV
                     ALPADINDPGTTSVYLRWVMTSNESISGADVVATGISKIDDIIVTGTYMAG"
     gene            complement(1191..2147)
                     /locus_tag="PLI16_02160"
     CDS             complement(1191..2147)
                     /locus_tag="PLI16_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83394.1"
                     /translation="MKSKINSFSVLCAQGFKYMVFCFVSLVLFTACKNDEDLLPPTIS
                     LIFESNTAHDGDTAAIGQPLRFKVQAEGLDANLTNFTVKKLYNGTVKTVLDSGMNSTG
                     FVTALTFYQNVEDAVEWQFAVMDRERQEASISLKIFKDPNSQFGGIYEFNNIRMGYQL
                     NTTYGQYFLPLVNKVYFADSAAMYQSQVDFLTYFNWKDDNGILKPSPTFSSPGEEPSA
                     TGELYDLYYPYLTGWGTRNYTKYDIRADNGVTAEGFENAHNDSLLIVSYDDVWGKKKY
                     KWATDGTFIPFQTAAGKKGIIRVHQADLDTTGSILFSMKIQM"
     gene            complement(2295..4097)
                     /locus_tag="PLI16_02165"
     CDS             complement(2295..4097)
                     /locus_tag="PLI16_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83395.1"
                     /translation="MKAAQKKSPPPHKAAKKVVALQAKNPKEQWWFLLIMVVSCVIIY
                     FPTLKYSVDVWDDPYYITNNTSVHSFANFSQLFTRFYVGNYQPLTILSYMIEWSLVKA
                     NPFLYHLDNMLLHLLNSLLSYVLIVKLTKNRFLAFVTGLLFVVHPLHIESVAWASQRK
                     DVLYSLFYFISYIFYLDYREKKKNSLLIISLAAFVLSCLSKGMAVVLPAVLVLTDFCF
                     DKKFSYKTLLNKIPYVMISLFFGLFAVFVQKESGAVGFTSKDIFSLSDKFFFINYSFF
                     FYIMKLVWPFHLSGLYPYPLQSGDLPLMYYLSLPLNLLIFGALIYSLKFSKKVLFGLG
                     FYAINVVMILQILSVGSAIAADRYFYMASVGLFYLLGLGLEKTNTMKTPLNGKVLKFF
                     VLGCLAVFFTIVSVGRVPVWKNAGTFWTDVTLKYPEIDVAWYNAGAYFYSANNQEQAL
                     KYFDKAISINPNHLDALKWRSMLYSQKNDFQKSLEDNLKIIALDSNNSRAYAAAGEIY
                     GKNLNNIDKSYWYLSKAIEKDPANEAALCNMGVVFHIRGDGEGAFTYFMKAYEINPDN
                     PFTLSNLASYYSLHGDTLKARQFSQKAKQLAGTK"
     gene            complement(4144..5136)
                     /locus_tag="PLI16_02170"
     CDS             complement(4144..5136)
                     /locus_tag="PLI16_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOH83396.1"
                     /translation="MKKFILLILTAIVFTCSCGNSNRDNNQSEEETSNLKGKLVIFHA
                     GSLSYPVKAICDSFKVSHPQVEILTEAAGSKDCARKISDLGKRCDIMLSADYKVIDNL
                     LIPEWADWNIKFASNEMSIVFTDRSKYAADINKYNWYNILLKDDVIYGRSDPESDPCG
                     VRSLLTMMLAEDYYNIPGLSKKMEAKDKNYIRPKETDLIALLEAKAVDYIFLYRSVAQ
                     QHNLKYLLLPDSVNLRNEALGDYYGTVSVETTGKKPGEKIRETGSPMIYGLTIPKSCE
                     NEELALAFLEFFLQADGGMAIMEASGQPSVVPAICEQFNKVPEKLKKFALPPKN"
     gene            5306..5389
                     /locus_tag="PLI16_02175"
     tRNA            5306..5389
                     /locus_tag="PLI16_02175"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5340..5342,aa:Leu,seq:gag)
     gene            5576..5659
                     /locus_tag="PLI16_02180"
     tRNA            5576..5659
                     /locus_tag="PLI16_02180"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5610..5612,aa:Leu,seq:cag)
     gene            5777..6892
                     /locus_tag="PLI16_02185"
     CDS             5777..6892
                     /locus_tag="PLI16_02185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83397.1"
                     /translation="MKKAIVSVINDLVTDQRVDRTCNTLVSLGFDVTLVGRRKKNSYL
                     LPKRNYGMFRFSMLFEKGPLFYAFFNVRLFFFLLFKKTDLLFANDLDTLLPNYLVSRI
                     RKIPLVYDSHEYFTGVPELTGRPMVQKIWKSIERAIFPKLKAVITVNQSIADLYRTEY
                     HKELTIVRNIPETRPLNNIKNRNELNLPENKKIMILQGSGINIQRGSEEAVEAVKYIE
                     NACLLIVGDGDVLPLLKKYVAENKMEDKVIFIPKQSPENLIHYTANADLGLTLDKDTN
                     INYRFSLPNKLFDYIHAGVPVLASPLVEIQKIITEYNIGCCIENHNPKHIAEKMQFML
                     ADSPDRENWKKNLIFAAKQLNWEEEQKKLIAVLQHHA"
     gene            6885..8009
                     /locus_tag="PLI16_02190"
     CDS             6885..8009
                     /locus_tag="PLI16_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83398.1"
                     /translation="MPDQHLHIIAFDIPVPVNYGGAIDIYYKLKSLKKAGVKIHLHCF
                     EYDRKPSPLLDDLCVEVNYYKRDNSKTKLFSSLPYIVATRYSENLLTNLLKDDYPILF
                     EGLHTCYLLNNKKLKNRKKIVRTHNIEHEYYSSLARVEKNMFRRYYFLNEASKLARFE
                     KQLDHANGIAAISMNDAAHFSRKYKKVRTISAFHSNEQITSKPGKGTFALYHGSLEVG
                     ENNEAALFLVNEVFNDLKIKFFIAGNKPSSELKTAVSGKKNIFLKMGISSEEIYDLVS
                     EAHVNILPTFQATGIKLKLLAALFRGRFCLVNTPMIEKTGLESLCICRNSADEFKKEL
                     ISLFNKDYDLSENKKREEILMHNGFSNDYNAAALVDLIFG"
     gene            complement(7996..8703)
                     /locus_tag="PLI16_02195"
     CDS             complement(7996..8703)
                     /locus_tag="PLI16_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH83399.1"
                     /translation="METHLEISSNTIIQLEKVSVFQNEVLVLANVNMAIERGEFVYLI
                     GPTGRGKSSLLKLIYAQLPARDGVARVASYDLRTIKTKEIPYLRRKLGIVFQDFQLLT
                     DRTVNENLLFVLRATGWHDKHDMQQRITEVLDKVGLTGKGPKMPNQLSGGEQQRVGIA
                     RALLNHPEIIIADEPTGNLDPATSEDILELLMNISKTGTAVLMATHDVLLFKRFPART
                     LRIDDGRVFEYQPANQK"
     gene            complement(8880..>9253)
                     /locus_tag="PLI16_02200"
     CDS             complement(8880..>9253)
                     /locus_tag="PLI16_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOH83400.1"
                     /translation="VEITATYPAGMIESTADNLVEAANGENEEWTELYPEFAAIAEEE
                     GFTQIATAFKLIAKIEAEHEARFLKLLRNVEDNRVFESEEPEEWVCRKCGYVHKGKKA
                     LENCPVCKHPKAYFEKKATNY"
BASE COUNT         2810 a         2091 c         1785 g         2567 t
ORIGIN      
        1 cccggccatg taagtaccgg taacaatgat gtcatcaatt tttgaaatgc ccgtcgctac
       61 cacatcggca ccgctgatgc tctcattgga agtcatgacc caccgcagat aaaccgaggt
      121 tgttccggga tcattaatat cggcaggtag cgccacttcg ttgagcacac cggtggtcca
      181 gtcgttggca acggtaatat tacctccggg aacatcggtc catgtacctg agccgcccaa
      241 tttgtactgc agtttccaga atttggggcc cgcataggtt tgtcccgaac gctgtttcga
      301 ccaaactttc atatcataat agtcgtcggc tttaaatttt attgaccaga acttaaaatt
      361 agcgccgttg tcccagccct cagccgtggc agcataatca gtagcgccat tggtatatgt
      421 cagggttctt acttctgccg aatcattctc tgccctgata tcataggtca ggttgttggc
      481 aagaccataa tctgcattaa attccacatc ggtagtatcg gcaaagttga aaccgattat
      541 ggtatctccg gtggccacgg gcagatccgc ggtgcccata ataataatat tgtcaatagc
      601 cgatttgcct gttggcagca cagggttcat atcaagggca atatttgatg tcatgatcca
      661 gcggatatag atggaggtgc cgccaggaac agctattgga atcgggagcg aatcaaccac
      721 gcccatattc caggcgctgt tgcagaccac tgtaccacca gggacattcg tccatggaat
      781 ggaaccccat ttgtattgta cacgccagtc tctggggcca tacagggtgc catcagacat
      841 ctgttttgaa tacaatacca aatcatgata accttctgct ttaaatttaa tcgaccagaa
      901 ttttgtgctg tcgccattat cccatcctgc tacttcttct gcataggtac catcaccggg
      961 aaccatggtg gtgactcccg taccgccctc atgcatcagt gttcttgtgc cgatgttact
     1021 gcttattcct gcattggcaa cagagtctgc aagggtacct gtcgggaaag tccaggccac
     1081 aatggtatcc tgtgcccgca tggtgaaaac tgtcagtatg cataacatac ctactaatag
     1141 taattgtttt ttcatttttt ctgtttttag ttacatttat tatttaatat ttacatttga
     1201 attttcatag aaaacagtat gcttccggta gtatcaagat cggcctggtg cacccgtatg
     1261 ataccttttt tccctgcggc ggtctgaaag ggaataaaag tgccatccgt agcccattta
     1321 tattttttct tgccccaaac atcatcataa gatacaatca gcaggctgtc gttatgcgca
     1381 ttttcgaagc cttcggcggt aacaccatta tctgcacgta tgtcgtattt ggtgtaattt
     1441 ctggtgcccc agccagtaag gtaaggataa tacaaatcgt aaagttcgcc tgtggccgat
     1501 ggttcttcac ctggggagga gaacgtaggc gaaggcttca atatgccatt atcatctttc
     1561 cagttgaaat acgtaagaaa atcaacctgg ctctgataca tagccgcaga atcggcaaaa
     1621 taaactttgt ttaccaaagg caggaaatac tgtccgtaag tagtgttcaa ctgatacccc
     1681 atacggatgt tgttaaattc gtaaatacca ccaaactgcg aattggggtc tttaaatatt
     1741 ttaagagaga tgctggcttc ctgacgttcc ctgtccatca cggcaaactg ccattctacg
     1801 gcatcttcca cattttggta aaaggtcaat gcggtaacaa agcctgtcga attcataccc
     1861 gaatcaagca ctgttttgac agtaccatta tagagttttt ttacggtgaa attggtcagg
     1921 ttggcatcca ggccttcagc ctgcacttta aatctcaggg gctgccctat ggctgccgta
     1981 tcgccgtcgt gggcggtgtt tgattcaaag ataaggctaa tcgttggagg cagcagatcc
     2041 tcatcatttt tgcaagcagt aaataacacc agcgaaacaa aacaaaacac catgtactta
     2101 aagccctgtg cacacaaaac ggagaaacta ttgatcttac ttttcatgct tgaaattatt
     2161 ttgtaataaa tgctgtttat tttttagtta tgcatcgatt tacatatcgg gtccaaaatt
     2221 aactaaaaaa gtcataccgc aaaaaagttg aaataataaa aacgatcatt agcattagca
     2281 ttaacaataa ccactcattt cgtacctgca agttgttttg ctttttgaga aaactgtctt
     2341 gctttaagcg tatcaccatg cagcgaataa taagaggcca ggttggagag cgtaaaggga
     2401 ttatcgggat taatctcata agctttcata aaataggtga aagcaccttc tccatcacct
     2461 ctgatgtgaa aaaccacacc catattgcac agtgctgctt cgttggcagg gtctttctca
     2521 attgccttcg ataaatacca atagcttttg tctatattgt tgagattttt gccgtaaatt
     2581 tcacctgcag cagcataagc ccgcgaatta ttggaatcca gggcgataat cttcaagtta
     2641 tcttccaggg atttttgaaa atcgtttttt tgagagtaca acatactgcg ccatttcaaa
     2701 gcatccaggt gattcggatt gatgctgatg gccttatcga agtacttcag cgcctgctcc
     2761 tgattattag ctgaataaaa ataagcccct gcattatacc aggccacatc aatttctgga
     2821 tacttgagtg taacatcggt ccagaatgtg cctgcatttt tccaaaccgg aaccctgccc
     2881 accgacacta tggtaaaaaa aacagccagg catcccagta caaaaaattt caatactttt
     2941 ccattcaaag gagttttcat tgtattagtt ttttccaaac ccaggcccaa cagataaaat
     3001 aaccccaccg aagccatata aaaatacctg tcggcagcaa tggccgaccc caccgaaaga
     3061 atttgcaaaa tcattaccac attgatggca taaaaaccaa ggccaaaaag cacctttttt
     3121 gaaaatttca atgaatagat caatgcaccg aatattaaaa gatttaaggg gagggataga
     3181 taatacatta gtggcagatc gcccgattga agaggataag gatacaggcc tgataaatgg
     3241 aagggccata ccaacttcat tatgtaaaaa aagaaactgt aattaatgaa gaaaaattta
     3301 tcactcagcg aaaaaatgtc tttggatgta aaacctacag ctccactttc tttttgcaca
     3361 aaaaccgcaa aaagtccaaa aaacaacgat atcatcacat aaggaatttt gttgagcagt
     3421 gttttgtacg aaaatttttt gtcaaagcaa aaatccgtca acaccagcac ggcaggtaaa
     3481 accacagcca tgccttttga caggcacgac aacacaaagg ccgccagcga aattatcagc
     3541 aggctattct ttttcttttc acggtaatca agataaaaaa tgtagcttat aaaataaaaa
     3601 aggctgtaga gcacatcttt gcgctgcgag gcccatgcca ccgactcgat gtgcaacgga
     3661 tgcactacaa acagcaggcc ggtaacaaat gccaggaaac ggtttttggt aagtttcact
     3721 atcagaacat agctcagcaa agaattcagc aggtgtaaca acatattatc cagatggtac
     3781 aggaaaggat tggctttaac cagcgaccac tctatcatat acgacaatat ggtcaacggc
     3841 tgataatttc caacataaaa acgggtaaaa agctggctaa agtttgcaaa tgaatgaacg
     3901 gatgtattgt tggtaatata ataaggatca tcccacacat caacggaata tttcagcgta
     3961 gggaaataaa taatcacaca ggataccacc ataatcagca aaaaccacca ctgttctttt
     4021 ggattttttg cctggagcgc gaccaccttt tttgctgcct tgtgtggcgg tggtgatttt
     4081 ttctgagctg ctttcatagt gttgccatac acataaatag agcctcaaaa ataagaaaat
     4141 cagctaattt tttggaggaa gggcaaactt ttttagcttt tcgggaactt tattaaattg
     4201 ctcgcatatt gcaggcacca ccgaaggttg tcctgaagct tccattatag ccatacctcc
     4261 atcggcctgg agaaaaaact caagaaaagc cagagctaat tcctcatttt cgcaactttt
     4321 gggtatcgtc aggccataaa tcattgggga gcccgtttca cgaatctttt ctcccggctt
     4381 ttttcctgtt gtttccacgc tgaccgttcc atagtaatcg cccagcgcct cattcctcag
     4441 attcacagag tcgggaagca gaaggtattt caaattatgc tgctgtgcaa ccgatcggta
     4501 taagaaaatg taatcaaccg ctttggcctc gagcaaagct atgaggtcgg tttccttcgg
     4561 acggatatag tttttgtctt tggcctccat tttttttgac agacccggga tgttataata
     4621 atcttcggca agcatcatag taagcaagga acgcaccccg caaggatcgc tctccggatc
     4681 gctgcgacca taaataacat catccttaag caaaatatta taccagttat atttattgat
     4741 atcagccgca tattttgatc tatctgtaaa aacaatagac atctcattgc tggcaaattt
     4801 aatgttccag tcagcccatt cgggaatcaa caggttgtca atcactttat aatccgccga
     4861 cagcataatg tcgcaccttt tccccagatc gctgattttt cgggcacaat ccttactgcc
     4921 ggcggcttca gtcaaaatct ccacctgcgg atggctcacc ttgaaagaat cgcatatagc
     4981 tttcaccgga taagaaagac tgccggcatg aaaaatcacc aatttacctt tgagattgct
     5041 agtttcttct tccgactgat tattatccct gttagaattc ccgcatgaac aggtaaacac
     5101 aatggcagtc aaaatcagta aaatgaactt tttcatattt cagctatttg tagcaaaaat
     5161 aatacttaat tggatatgta caccattgcc taaccaacag attgcaggta ttcattgaaa
     5221 aaaacaacgt ggaaacaaaa ttttcggaaa atatgtattt tttgtttgtt gttagataaa
     5281 aatgattact tttgcaccca cctttgccca ggtggtgaaa ttggtagaca cgccagcttg
     5341 aggggctggt gcccgtacgg gtgtgcaagt tcgagtcttg tcctgggcac ggttgagtta
     5401 acaatgtaca atgatcgatg atcattaaca taaataagtt ctttgaaggt attacacaat
     5461 aatactaaaa aacactgaaa gaataaaaac aaaatcagaa agtaaaattt aacaactgac
     5521 ggataattgt taattgttaa ttgttaattg ttaattgaca actgttcatt gtatcgcccg
     5581 gatggcggaa ttggtagacg cgctagtttc agggactagt attcgcaagg atgtgcaggt
     5641 tcgagtcctg ttccgggcac aaaaagcaga cgaaagtctg ctttttttat ttctcatcta
     5701 cttgaaaatc cataccattt gaaagaagtc ttttcaaggc atccatattt ttattttact
     5761 tttgcaaaaa aacgttttga aaaaagccat tgtctcggta atcaatgatc ttgttactga
     5821 tcagcgtgtt gacaggacat gtaacaccct tgtatcacta ggctttgatg taaccctcgt
     5881 tggcagacga aaaaaaaaca gctacttact gcccaagcgc aattatggaa tgttcaggtt
     5941 ttctatgttg tttgaaaaag gccccttgtt ttatgctttt ttcaatgtac ggctgttctt
     6001 ctttttgctt ttcaaaaaaa ccgaccttct ttttgccaac gatctcgaca ccctattgcc
     6061 aaattacctg gtttccagaa taaggaaaat ccctttggtt tacgacagcc acgaatactt
     6121 taccggagta cccgaactca ccggcaggcc aatggttcag aaaatctgga aaagcatcga
     6181 acgtgctatt ttcccaaaac taaaagctgt catcacggtt aaccaatcca tcgccgacct
     6241 atacagaacc gaataccata aagaacttac aatcgtcaga aacatacctg aaacaaggcc
     6301 actaaacaat ataaaaaaca gaaacgagct gaaccttcct gaaaacaaaa aaatcatgat
     6361 cctgcaaggc tcgggcatca acatacaacg cggctccgaa gaagctgtag aagctgtgaa
     6421 atacatcgaa aatgcctgcc tgctgatcgt gggtgacggg gatgtattgc ctttattaaa
     6481 aaaatatgtg gccgaaaaca aaatggaaga caaagtgatt tttattccaa aacaatctcc
     6541 cgaaaacctt attcattata ctgccaatgc cgatcttggc ctgaccctcg acaaagatac
     6601 caacatcaac taccgtttca gtttaccaaa caaactgttc gactacatcc atgccggcgt
     6661 acctgtactc gcctcccctt tggttgaaat acaaaaaatt atcacggaat acaacattgg
     6721 ctgttgtatt gaaaaccata accctaaaca catagccgaa aaaatgcagt ttatgcttgc
     6781 cgattccccg gaccgtgaaa actggaaaaa aaatcttatt tttgccgcca aacaactaaa
     6841 ctgggaagaa gaacaaaaaa aacttatagc cgtacttcag caccatgcct gaccaacatc
     6901 ttcatatcat agctttcgac atacctgtac ccgtcaacta cggtggtgcc attgacattt
     6961 attacaaatt aaaaagtctg aaaaaagccg gcgtaaaaat ccacctgcat tgttttgaat
     7021 acgaccgcaa accttcgccc ctgctcgatg atttatgcgt ggaagtaaat tattacaagc
     7081 gcgacaattc caaaaccaaa ctcttcagca gtctgccgta cattgtagct acacgctatt
     7141 cggaaaacct tttgaccaac ctgctcaagg atgactaccc catactattc gaaggactgc
     7201 acacctgcta tctgctgaac aacaaaaaac tgaaaaaccg aaaaaagatt gtccgtactc
     7261 ataacattga acacgaatat tacagcagcc ttgcccgtgt tgaaaaaaac atgttcaggc
     7321 gttattattt tctgaacgaa gccagcaaac ttgcccgttt tgaaaaacag ctcgaccatg
     7381 ccaacggcat tgcagccatc tcaatgaatg atgctgcaca tttttcacgc aaatataaaa
     7441 aagtacgcac gatctcagct tttcactcca acgaacagat tacctccaaa ccaggtaaag
     7501 gaacctttgc cttgtatcat gggagcctcg aagtaggcga aaacaatgaa gcggccttat
     7561 ttctggttaa tgaggtattc aacgatttaa aaataaagtt ttttatcgcc ggcaacaagc
     7621 caagcagcga gctcaaaacg gctgtgagtg gcaaaaaaaa catcttcctt aaaatgggca
     7681 tttccagtga agagatttac gacctggtat ccgaggccca tgtaaatatt cttcctacct
     7741 ttcaggccac aggcataaag cttaagctcc tagctgcgct gtttcgcgga aggttttgct
     7801 tagtcaacac tcccatgata gaaaaaacag gactcgaaag cctgtgtatt tgcagaaatt
     7861 cggcagatga attcaaaaag gaattaataa gcctttttaa caaagattac gacctcagcg
     7921 aaaacaaaaa aagagaagaa attttgatgc acaacggctt ttccaatgat tacaatgccg
     7981 cagccctcgt agatcttatt tttggttagc aggctgatac tcaaacaccc tcccgtcatc
     8041 gatacgcagt gtgcgtgcag gaaatctttt aaataacagt acgtcgtggg tggccatcag
     8101 tactgcagtg cctgttttgc tgatattcat aagcaactcc aaaatatctt ctgaagtagc
     8161 cggatcgagg ttaccggtag gctcatcggc aatgataatc tcgggatgat tgagcaaggc
     8221 ccgggcaata cccacccgct gctgctcacc tcctgaaagc tggttaggca ttttaggacc
     8281 tttgcctgtc aaacccacct tgtccaaaac ctcggtgata cgttgctgca tatcgtgttt
     8341 atcatgccaa cccgtggcac gcagcacaaa gagcaggttt tcgttcacgg tacggtcagt
     8401 aagcaactga aaatcctgaa aaacaatgcc taactttctt ctcaaatatg gaatttcctt
     8461 tgtcttgatg gttcgcaaat cataggaagc caccctggca acaccatctc tggccggaag
     8521 ctgcgcgtaa ataagtttca gcaggctgct tttgccgcgg cccgtcgggc ctatcagata
     8581 tacaaactct ccacgctcaa tggccatatt gacattggca agaacaagaa cttcattctg
     8641 aaaaactgag actttttcaa gttgtatgat ggtattactg cttatttcaa gatgtgtttc
     8701 cataaataaa attgaatttt caaaaataag ttttttttct atacggcaaa aaatcaaaat
     8761 cattatctat taacaatgaa tcacattgct gcaagacaaa gcttttttgc agtagctgct
     8821 tcgatatttt caggacaggc aaactgcaaa caagattcaa aaatcgctgt tattaaaaat
     8881 cagtaattcg tagccttctt ctcaaaatag gctttgggat gtttgcatac aggacagttt
     8941 tccagcgctt ttttcccttt atgaacataa ccgcatttgc ggcaaaccca ttcttcgggt
     9001 tcttcgcttt caaaaactct attatcctct acattcctca gcaatttcaa aaaacgtgct
     9061 tcgtgttccg cttcaatttt tgcgataagc ttaaaagccg ttgctatctg tgtaaaacct
     9121 tcttcttcgg caatcgctgc aaattcaggg tacaattctg tccattcttc gttttcaccg
     9181 ttggcagcct ccaccaggtt atcggcagtg ctttctatca ttcctgccgg ataagtagcc
     9241 gtaatttcaa cgg
//
LOCUS       DAOSFP010000042         9235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6370_length_9235_cov_3.556536, whole genome shotgun sequence.
ACCESSION   DAOSFP010000042 DAOSFP010000000
VERSION     DAOSFP010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6370_length_9235_cov_3.556536"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLI16_02205"
     CDS             <1..196
                     /locus_tag="PLI16_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83401.1"
                     /translation="KQSIDDLVDQLIDCLIADKQMKNANITSKDITRVKSVLKNRLIS
                     MYHVRIEYPKMNAQNDGSTE"
     gene            complement(233..844)
                     /locus_tag="PLI16_02210"
     CDS             complement(233..844)
                     /locus_tag="PLI16_02210"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOH83402.1"
                     /translation="MIFSTAYLPPITFFVYASQAKEIRIEACENFIKQTYRNRCYIYS
                     ANGKLSLSIPLDHCRKPQLPLKEVKISYATPWNLIHWRAITAAYNKSAYFLYYRDDFE
                     KFFTLKYSWLIDYNHELLTVCLKQLRLNKEITYTESFHSTYDSGDLRAKINPRNEPEF
                     CFVPYTQVFDTKNGFISNLSIIDLLFNCGPDSLEYLEQTASTP"
     gene            1000..1881
                     /locus_tag="PLI16_02215"
     CDS             1000..1881
                     /locus_tag="PLI16_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83403.1"
                     /translation="MKKSFLLVFSLVLSSGLFAGEAVSTSLFDAVQKNLVKVVFKGRG
                     YTSENRSAHYGKCISLHLKNVSSQRLELSLEAGRKLHCYYDSVQDMIVSQSEMFALGP
                     GGSSDFTINAFCIEKNETAPKENSVYTMTALAGGYLLQLVQLIESLGCQDIMGQQAVW
                     VLTDNVSPENVKGGDREKEKKLKAFVEFALKKIQSGKIDGVIYDYSFPDKMAGGFKIA
                     GQINWDMPYSGTVTLGVYDNTGKKVADIFAGVPYKKGFQTFTYELASSLFREGELYWL
                     KVISNGERLKEVAITMK"
     gene            1951..2694
                     /gene="rlmB"
                     /locus_tag="PLI16_02220"
     CDS             1951..2694
                     /gene="rlmB"
                     /locus_tag="PLI16_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOH83404.1"
                     /translation="MEKELLIYGIRPVIEAINAGKEVEKVLIQNGLRSELFHELRVLL
                     AQHSIPYQFVPAEKLSRVTKKNHQGVIGYISSISYSDIEKIIPFLFDQGKSPLVLILD
                     RITDVRNFGAIARTAVCAGCDAIVIPYYNSVRVSSDAIKSSAGALHKVPVCRSSSLIK
                     TVEFLKSCGLQIVACSEKTQQPYYEADMVVPTAIIMGSEEDGITDVLLKSADHKVKIP
                     MAGDIASLNVSVACGIILFEACKQRSKEN"
     gene            2808..3695
                     /locus_tag="PLI16_02225"
     CDS             2808..3695
                     /locus_tag="PLI16_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83405.1"
                     /translation="MKKIYVTLLILFSCFSLNAQVVNGGFENWINDSVPETWNGTISV
                     DVIIQNYEFNTVSKTNDSYSESFAAVVSTGKTIPYVEILLPGIISYGNNQIKLNISDF
                     SLVITSIGGIPINVIPTKIKGYYKYSGVNNDTMAIYADCFYNSILIGQGVFSDSCDQT
                     LYVPFEIDIAYSEEEVPDMINIIATSSGGYMPQAGSMLYLDDLSMEYTMAGISESLPL
                     DMLSIYPNPTTGLLTLKLNSGETNIVNIYDYTGKLIETTGIDGPYFTLDMRSYVNGSY
                     FVEVVNSGGRNTQKVVLAR"
     gene            3851..4729
                     /locus_tag="PLI16_02230"
     CDS             3851..4729
                     /locus_tag="PLI16_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83406.1"
                     /translation="MKKFYLFFLTVFFVFSATAQIPNAGFETWTNDTTPVSWNGAMSV
                     TVMGFYNFDLATVEKSTDAHNGTYAAMLTSDRTIPIVGTLLPGILSYGKNVYDILSGI
                     QVSGGIPVTVKPLKIKGYYKYANVNNDTMGIMGICYKSGDTIATGGLMSASTVSSYTY
                     FEFDLDYTQSLMPDTFNIIAVSSAGAAAQTGSALYLDDIEVVITGAGFEETISVDDLV
                     KLSPNPTQGLLTVELMPNETNFITVYDFSGKPVLAQQPVINRTKLDMSAFSNGVYFIE
                     IINSNGRYTKKLVVAK"
     gene            4837..5292
                     /locus_tag="PLI16_02235"
     CDS             4837..5292
                     /locus_tag="PLI16_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83407.1"
                     /translation="MEEKIKLKQSIIESCRNIILETINHLTREMEEHQQLANDYGPPK
                     DRYDSFRSQMLRRRDMYAQQLHKANQELTALEKIDASEIKNEVVFGAVVITSQQKLFV
                     SISVGKLSVGDTLYYAVSPNVPVYNALKGLKKGDTAAFNNTVIEVLDVF"
     gene            5282..6388
                     /locus_tag="PLI16_02240"
     CDS             5282..6388
                     /locus_tag="PLI16_02240"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83408.1"
                     /translation="MFFDPNCPEPCNGCRHRYFTEEESLQKKSAFLQKKLSVWNDYFN
                     VIRSVHGEARRHYRKKVCLHTVYNGKEWEFGMISHRQFVATPDCPVQYERIGEVIRLL
                     SKVLPPPEKFPLHFFIQSGNQVMLVLKTACMPDNSWLGSVVEEQLRQLGVEGCWLHLN
                     PSAGNKIFMKHNFSLLFGSDVSIDEQGMCYGPMSFQQLIPELYFDSLHEAAFFLCPGR
                     HAMVIDLYCGTGYSMKKWVSAGSLTAGVELNGEAVACALKNVPGALIFRGKCQDRLPQ
                     LIQWADEHKAMKKLLYVNPPRTGLEQPVLQWIAHSYQPERMAYLSCSAGTLARDLDFL
                     CSKGFDVEKIIPYDFFPQTLHVECLVLLSRTLGK"
     gene            complement(6493..7581)
                     /locus_tag="PLI16_02245"
     CDS             complement(6493..7581)
                     /locus_tag="PLI16_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH83409.1"
                     /translation="MNAIIENYKRNIIQIASPYGTGTGFYNADNHLFITNYHVIKDND
                     EVIISGRGFKKTTAKVLYFDPLYDLAFIQAPENIDLQKALLSSVPVSEGDNIIAIGHP
                     YGLKYTATKGIVSKASRLYNEISYIQIDAAINPGNSGGPLVNMDGEVVGVNTFIIAQG
                     ESLGFSLPSKYLQETLDAYASMNKQKAVRCKSCKKILLENQIDGMYCSNCGTEIDMNI
                     LRPKPYIPAGAAYKVEQVLEKLGINVKETRIGLNTWELEHGSVKIKIIYNPDTRFIVA
                     DAWLCNLPKENIMMVYEYLLKENYDSEGLVFSVNNQAILLSFISYEDDLSIDTGCELL
                     KNLMYKSDYYDDMLIEKFQAQPITTEEN"
     gene            complement(7588..8919)
                     /locus_tag="PLI16_02250"
     CDS             complement(7588..8919)
                     /locus_tag="PLI16_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83410.1"
                     /translation="MSFFKKVFSSSDNNNTGNSSVSNNVAVGRYTEINKKPAQSEYWK
                     KSQDDFSQKKYLDAFDKLLNYMLDPAVENIKISRREDALNFELLQGSKLIFGKADEKN
                     FSAEARIARYDRPSVAFLRKLMNMNYMLQYNRFAIKDDIIYLKFDSKTIDASPNKVYY
                     ALRELALKCDKIDDNLIGEFEMLHAIDNGHTMDIPENFKEIKYKYLHLWIDEALNKIL
                     SLNEDRMSGGISYILLALLFRIDYLLQPQGALFEDIEKINGIYNAKDNKSTLERNRLM
                     IDELKRVKSKSKEEIFKNLYDVKITFGYVPATSHKQFYEFILEQFKNTNWYYDNRYPD
                     IVTSIYEWIIGYSLFYYGLFPATYDFLKLAYTVMQPAYFAEMGAPLNLYNPANNTFDK
                     TAIEKEIQKIVKAHQPEYPKIAILMENIRYNSMNDFLYTYLNELTYLNFTK"
     gene            9051..9142
                     /locus_tag="PLI16_02255"
     tRNA            9051..9142
                     /locus_tag="PLI16_02255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9085..9087,aa:Ser,seq:cga)
BASE COUNT         2512 a         1803 c         2107 g         2813 t
ORIGIN      
        1 caaacaatct attgacgatc tggtggacca gttgattgat tgcctgatag ccgacaagca
       61 aatgaaaaac gctaatatca cttcaaaaga tatcacccgt gtaaagtcgg tgttgaaaaa
      121 caggcttatc agcatgtatc atgtcaggat cgaatacccg aaaatgaatg cccaaaacga
      181 tggttcgact gaatagctga aaaattttag tttgctactt tgacagggcg ttttatggtg
      241 tcgaggctgt ttgttccagg tattccagac tatcgggccc gcagttgaac agcaggtcta
      301 taatactgag attactgatg aaaccatttt tagtatcgaa gacctgagtg tagggcacaa
      361 aacaaaattc cggttcattt ctggggttta tctttgcgcg aaggtcaccg ctatcataag
      421 tggaatgaaa gctttcggta taggtaattt ctttgtttag tcgcagttgt ttcaggcata
      481 ccgtcagcag ctcatgatta taatctatca accagctgta tttgagggta aaaaactttt
      541 caaaatcatc acgatagtaa agaaaatatg ccgacttgtt ataggctgcg gttattgctc
      601 tccagtgtat caggttccag ggcgtggcat agctgatttt tacctctttt agcggaagct
      661 gtggctttct gcaatgatct aaaggtatgc tgaggctaag tttgccgttg gcagaataga
      721 tataacagcg gtttcggtaa gtttgtttaa taaaattttc gcaggcttca atacgtattt
      781 ctttcgcttg ggaggcgtac acaaaaaacg ttatgggtgg caggtatgca gtagaaaaaa
      841 tcacaataaa aaatttccgg taaatttctg aaaatttcct tacaacagac aattttccgg
      901 ttaaaagtta cgtttcccgt ttgtaatcct tctatttaca atcccctaaa tctctgttat
      961 gtagaaaaaa tatacccgaa cccataaaaa acccctatta tgaaaaaatc ctttttatta
     1021 gttttttcgc tggtgttgag ttcgggtctt tttgccggcg aagcggtgtc aaccagtctt
     1081 tttgatgccg tacagaaaaa tcttgtcaag gtggtcttca aaggccgcgg ctacacctcc
     1141 gaaaaccgga gtgctcacta tggtaaatgt atttcacttc acctgaaaaa cgtcagctcg
     1201 caacggcttg aacttagcct cgaagccggc agaaagctgc actgttatta tgacagtgtt
     1261 caggatatga ttgtgtcgca atctgagatg tttgccctgg ggccgggggg aagcagcgat
     1321 ttcaccatca acgctttctg tatcgaaaaa aacgagactg cgccaaagga aaattccgtc
     1381 tataccatga ctgccctggc cggcggttac ctgctgcaat tggttcaact gatagagagc
     1441 cttggttgtc aggatattat gggacaacag gcggtgtggg tgcttaccga caatgtttcg
     1501 cctgaaaatg tcaaaggcgg ggaccgtgaa aaagaaaaaa agctcaaagc ctttgtggaa
     1561 tttgccttga aaaaaataca aagcggaaaa attgatggtg tcatttatga ttattccttc
     1621 cctgataaga tggcaggagg ctttaaaatt gcaggtcaga taaactggga tatgccttat
     1681 agcggcaccg ttacccttgg tgtgtacgac aatacaggga aaaaagtggc ggatattttt
     1741 gccggagttc cttacaagaa aggatttcag acttttacct atgagctggc cagctcgttg
     1801 ttcagagagg gtgaactgta ttggctgaaa gttatttcaa acggtgagcg gctcaaagag
     1861 gtggctatta ccatgaaata gcatgggagg atcattgtat attatctttt gataacggtt
     1921 ttatgcgtat gtttgcagca aatttatccc gtggaaaaag aattattgat ttatggtatc
     1981 cgtccggtga tagaagccat caatgccggc aaagaggtag aaaaggttct gatacagaac
     2041 gggcttcgtt cggaattgtt tcatgagctg agggtgttgc tcgcccaaca cagtattcct
     2101 tatcagtttg ttcctgctga gaagttaagc cgtgtgacta aaaaaaatca tcagggcgtc
     2161 atcgggtata tttccagtat ttcgtattcc gatattgaaa aaatcattcc ctttcttttt
     2221 gatcagggca agtcgccctt agtattgata ctcgaccgta ttaccgatgt gcgaaatttt
     2281 ggcgccatcg ctcgcacggc ggtttgtgcc gggtgcgatg ctattgttat tccatactac
     2341 aacagtgtgc gggtgagttc tgatgcaata aaaagctctg ccggcgcctt gcataaggtg
     2401 cctgtgtgca ggagcagcag ccttatcaaa acagttgagt ttctgaaatc gtgcgggctt
     2461 cagatcgtgg cctgcagcga gaaaacgcaa cagccgtatt atgaggcgga tatggttgtt
     2521 cctaccgcga ttattatggg gtcggaggaa gatggtataa cggatgtgct gctcaaatct
     2581 gctgatcaca aagtgaaaat tcctatggcc ggcgacattg cctccctgaa tgtttccgtg
     2641 gcctgtggca tcattttatt cgaggcctgc aaacaaagat caaaggaaaa ctaacctgcc
     2701 atcggggata ttgtcgcaag tggcattttt ctaagatttt acttgctttt ttgccttttt
     2761 ttcattatat ttgtggtacg tacgaaccca tataatgcat ccacgccatg aaaaaaattt
     2821 atgttacctt attaatttta ttcagttgtt tttcgttaaa cgctcaggtg gttaacgggg
     2881 gatttgaaaa ctggataaac gattcggtgc cggaaacctg gaatggcact atcagtgttg
     2941 atgtcataat tcagaattac gaatttaaca cggttagcaa aaccaacgac agctattccg
     3001 aatcatttgc cgctgtagta tctacaggaa aaaccattcc ctatgtggaa atccttttac
     3061 cgggtattat ttcatacgga aacaaccaaa tcaaattaaa catttctgat ttctcgctgg
     3121 taattaccag catcggcggt attcccatca atgtaatacc aacaaaaata aaaggctact
     3181 acaagtattc aggagtcaat aatgatacga tggctattta tgcggattgt ttttacaatt
     3241 ctattttaat tggacagggt gttttttctg attcctgtga ccagacactt tatgtgcctt
     3301 ttgaaatcga cattgcatac agcgaggagg aagtgcccga catgattaac attattgcta
     3361 catcatctgg cggatatatg cctcaagccg gatctatgct gtatttggac gatttgtcta
     3421 tggaatatac catggccgga atatccgaat cactgccgct ggatatgctt tccatatatc
     3481 ctaatcccac cacaggctta ttaaccctga aattaaactc cggagaaaca aacattgtta
     3541 acatttatga ttataccgga aaacttattg aaacaacagg catcgacggt ccctatttca
     3601 cactggatat gcgttcgtat gtaaatggtt cgtatttcgt cgaggtggtt aattcgggcg
     3661 gacgaaatac gcaaaaagtt gttctggcaa gataagtgtg aaaattgcct gttaaaaggt
     3721 cattccatcg tggaatggcc tttttttatg attgttccgg caatattatt tctttagaaa
     3781 aaattgacat atattgccac ttttttttaa ttttgacttg ttttaaataa ttaattaaaa
     3841 aaatcatctt atgaaaaaat tctatttatt ctttttgaca gtattttttg ttttttcagc
     3901 aacagcacag attcccaacg ccggctttga gacgtggacc aatgatacca ctcctgtttc
     3961 ctggaatgga gccatgtccg ttaccgtaat gggattttat aattttgacc ttgccactgt
     4021 tgaaaaaagc accgatgccc ataacggaac gtatgcagcc atgttaacct ccgacagaac
     4081 tattcctatc gtaggaaccc tgctgcccgg catcctgtcc tatggtaaaa atgtttatga
     4141 tattttgagc ggtattcagg tttcgggtgg tattccggta acggtaaaac cattaaaaat
     4201 taagggttat tataaatacg ccaatgtaaa caatgatact atgggcatta tgggtatctg
     4261 ctataaaagc ggcgacacca tagctaccgg tggcttaatg tcggcttcaa cagtttcttc
     4321 ttacacttat tttgaatttg atttggatta tacacaaagc ctgatgcccg atactttcaa
     4381 tattattgca gtgtcttcgg ctggcgctgc tgctcaaacc ggctccgccc tgtatctcga
     4441 cgacatcgaa gtggtaatta caggtgcagg ttttgaggaa acaatttctg tcgatgatct
     4501 ggtaaaacta tcgccaaatc ccacacaggg tttgcttact gttgaattaa tgcccaacga
     4561 aaccaacttc atcactgttt atgatttttc gggaaagccg gtccttgcgc aacaacctgt
     4621 catcaataga accaagctgg atatgagtgc attttccaac ggcgtttatt ttatcgaaat
     4681 aatcaacagc aatggccggt acaccaaaaa gctggtagtg gcaaagtaag atatgaatcc
     4741 gttttaaaag gtcattctct gctactgaat ggcctttttt attatataga aattattatg
     4801 tactgtcctg atttatagac acatgaacta gaatttatgg aagagaaaat aaagcttaaa
     4861 caaagcataa tagaaagctg ccggaacata attctggaaa cgataaatca tctgactagg
     4921 gaaatggagg agcatcagca attggccaac gactatggac ctcctaagga ccgttatgat
     4981 tcatttcggt cgcagatgct gcgccggcgc gatatgtacg cacaacagct ccataaggcc
     5041 aatcaggagt taaccgccct tgagaagatt gatgccagcg aaattaaaaa tgaggtggtg
     5101 tttggcgccg tggtaatcac cagtcagcag aagctttttg tcagtatcag tgtgggaaag
     5161 ctgtctgtcg gtgacacgct gtattatgcc gtttctccca atgtgcctgt ttacaatgcc
     5221 ttgaaaggtt tgaagaaagg cgatacggca gcgtttaata ataccgtaat agaggttctc
     5281 gatgtttttt gatcctaatt gcccggagcc gtgtaacgga tgccggcaca ggtattttac
     5341 cgaagaagaa agtctgcaaa aaaaatcagc ttttctgcaa aagaaacttt cagtatggaa
     5401 tgactatttt aatgtcatca ggagcgttca tggcgaagcg cggaggcatt accgcaagaa
     5461 agtctgtctt cataccgtgt ataatggcaa ggaatgggag tttggcatga tttcgcacag
     5521 gcagtttgtg gcaactcctg actgtcccgt gcaatatgaa cgcatcggtg aggttatcag
     5581 gttgttatca aaagttttgc caccgcccga aaagtttccg ttacattttt ttatacaatc
     5641 ggggaatcag gtgatgctgg tacttaaaac tgcctgtatg cccgataatt cctggttagg
     5701 atcagttgta gaagaacagt tgcggcagct gggtgtggaa gggtgctggc tgcacctgaa
     5761 cccctcggca gggaataaga tttttatgaa acataatttt tctttgcttt ttggtagcga
     5821 tgtttctatt gatgagcagg ggatgtgcta tggtcccatg tcttttcagc aattgatccc
     5881 ggaattgtat tttgattcgt tgcacgaagc tgcttttttt ttgtgtcccg gcaggcatgc
     5941 tatggtgatt gacttgtatt gtggtacagg ctacagtatg aagaaatggg tgtcggcagg
     6001 aagcctcact gccggagtgg agctaaacgg cgaagcggtg gcctgtgccc tgaaaaatgt
     6061 tcccggggca ttgatatttc gcggaaaatg ccaggaccgt ttgccgcaac ttattcaatg
     6121 ggctgatgaa cataaagcca tgaagaaatt actatatgtc aatcccccgc ggacagggct
     6181 ggaacagcct gttttgcaat ggatagcgca cagttatcaa cccgaacgta tggcttatct
     6241 ttcatgcagc gcaggtacct tggcgcgtga tttggatttt ttgtgttcaa aaggttttga
     6301 cgtggaaaaa attatccctt atgatttttt cccgcagacg cttcacgtgg agtgcctggt
     6361 gctgttgtcg cgcacattgg gaaagtaggt ggctttagta gcgtcgtttt tcaggtatgc
     6421 aatgatgttg gttcactggt aaaaaaatta ccgtgaaaat aagaggttgt cctattttgt
     6481 aaagaaatca gattaatttt cttctgtcgt tattggctgt gcctgaaatt tttctatcaa
     6541 catatcatca taatagtctg atttgtacat caggtttttc aatagttcgc agccggtatc
     6601 aatagagagg tcgtcttcat acgagatgaa agacaacaag atggcctgat tgtttacact
     6661 gaacaccaat ccttcactgt cgtaattttc ttttagcagg tactcatata ccatcataat
     6721 gttttctttg ggcaggttgc acagccaggc atccgccaca ataaacctgg tatccgggtt
     6781 gtagattatt tttattttta cgcttccgtg ctccagctcc caggtgttga gcccaatccg
     6841 tgtttctttc acgttgatgc cgagtttttc aagaacctgt tccactttgt aagctgcacc
     6901 ggcaggaatg tagggcttgg gacggagtat gttcatatcg atctctgtgc cgcaattgga
     6961 acaatacatg ccgtcaatct ggttttccaa cagtattttt ttacatgatt tgcacctgac
     7021 ggctttttgc ttgttcatcg atgcataggc gtcgagggtt tcctgcaggt atttcgacgg
     7081 cagcgaaaaa cccaggctct caccttgtgc aataataaag gtgttgacac caaccacttc
     7141 gccgtccata ttgactaaag gaccgccgct gttgccggga ttaatggccg catcaatctg
     7201 aatatagctg atttcgttat agagcctgct ggctttggat acaatgccct tggtggccgt
     7261 atatttcagg ccgtaaggat ggccgatggc aataatatta tcgccttcag agaccggtac
     7321 ggacgacagc agtgcttttt gtaaatcgat gttttccggc gcctggataa aagccaggtc
     7381 gtacagcgga tcaaagtaca acactttagc agtagttttc ttaaaacccc tgccgctgat
     7441 gattacctcg tcgttgtcct tgataacatg gtaattggta ataaacaagt ggttgtccgc
     7501 attgtaaaaa ccggttccgg ttccataggg cgaggcaatc tgaataatgt ttcttttata
     7561 gttttctatt attgcattca taatgtgtta cttcgtaaag tttaaataag tgagttcgtt
     7621 taaataggtg tataaaaaat cattcattga attatacctg atgttttcca taaggattgc
     7681 aatcttgggg tattcgggct gatgcgcctt gacaattttc tgaatttctt tttcgatggc
     7741 cgttttgtca aacgtgttgt ttgccggatt gtagaggttc aggggtgcgc ccatttcggc
     7801 gaagtatgcc ggctgcatga cggtataagc cagcttcagg aaatcatagg tggcagggaa
     7861 cagaccgtaa taaaacaacg aataccctat gatccattca taaatggagg ttacaatatc
     7921 cggatagcgg ttgtcgtaat accagttggt gtttttaaac tgttcgagaa taaactcgta
     7981 gaattgtttg tggctggttg ccggcacata accaaaggtg attttcacat cgtagagatt
     8041 tttgaatatc tcttccttgc ttttgctttt aactcttttc agttcatcaa tcatcaggcg
     8101 gttacgctcc agggtgcttt tgttgtcttt ggcgttgtat atgccgttaa ttttttctat
     8161 gtcttcgaat aatgcgccct gtggctgaag caggtagtcg atacggaaaa ggagagcgag
     8221 taaaatatag gaaattcctc ccgacatcct gtcctcgttg aggcttaaaa ttttgttcaa
     8281 cgcctcgtca atccacaggt gcaggtattt atattttatt tctttgaaat tttcggggat
     8341 atccatggta tggccattgt cgatagcatg cagcatttca aattcgccga tcaggttgtc
     8401 atcaatcttg tcgcatttga gggccagttc gcgcagagca taataaactt tgtttggcga
     8461 agcgtctatg gttttgctgt cgaatttgag gtaaatgata tcgtctttta tggcaaatcg
     8521 gttatactgc agcatgtaat tcatattcat cagcttgcgc agaaaggcga cgctgggcct
     8581 gtcgtagcgg gctatgcggg cttccgccga aaaatttttt tcgtcggctt tgccaaagat
     8641 gagtttggag ccttgcaaca actcgaagtt cagcgcgtct tcgcggcggc ttattttgat
     8701 attttcgacg gccgggtcca gcatataatt gagcagttta tcgaaggcat cgaggtattt
     8761 tttctgcgaa aaatcatcct gagatttttt ccagtattcg gactgggcag gttttttgtt
     8821 tatttcggta tatctgccca cggcgacatt gttagaaacc gatgaatttc cggtgttgtt
     8881 attatctgac gaagagaata cttttttaaa aaaagacata atcgaaggat ttgtaaaatg
     8941 atttgttggt tttataagac caaaagtaag aaaaatcttt ttctgatatt taaagatggt
     9001 ttcagaaaat tgctaaatca gaattaaaat gtatttttgc agtcgatttt ggagaggtgg
     9061 cagagtggtc gattgcggcg gtctcgaaaa ccgttgtccg ccatccggcg gaccgggggt
     9121 tcgaatcccc ccttctccgc caatcaaaac ttcccgcgac agcgggattt tttatttaga
     9181 agaatattca aacttgtttg caaatattat gagaaataaa aaatcaatga ctacg
//
LOCUS       DAOSFP010000043         9036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6555_length_9036_cov_3.958245, whole genome shotgun sequence.
ACCESSION   DAOSFP010000043 DAOSFP010000000
VERSION     DAOSFP010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6555_length_9036_cov_3.958245"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PLI16_02260"
     CDS             <1..580
                     /locus_tag="PLI16_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HOH83411.1"
                     /translation="RKVVAAAAINETWLNELIWREFFMMILWHFPQVVTESFKKKYDD
                     ISWRNNEEEFARWCRGETGFPIVDAGMRELHATGWMHNRVRMITAGFLVKHLLTDWRW
                     GEAYFAQKLIDYELASNNGNWQWAAGSGCDAAPYFRIFNPAEQTRRFDPQHEYIKRWV
                     PEYTTKSYAQPLVEHNFARERALNTYKRSLLR"
     gene            complement(584..2086)
                     /locus_tag="PLI16_02265"
     CDS             complement(584..2086)
                     /locus_tag="PLI16_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOH83412.1"
                     /translation="MNQEILKLKPKSVWGYFDEIMQIPRPSGKEQKITAYLKAFGQKH
                     KLETLVDEVGNVLIRKPATKGKEKLPWVVLQSHVDMVCEKNSNITHDFEKDPIKAVVE
                     KGWLIAKETTLGADNGIGVAMQMAILADISIEHGPIECLFTVDEETGLKGAFSLKPDF
                     LKSRMLINLDSEEEGEFTIGCAGGGDTVARFLYKKEPVPAAHEAFLINVGDLQGGHSG
                     EDINKNRGNAVKLLNRILWNISKKFDARISYIKAGNLRNAIAREGKCIVVIPKKNTTA
                     FAAYFNEVARNIKDEYQTNEPQLTITCTGAKKPAQVLDIKTQQTLLNCLYACPHGVIT
                     MSPDIQGLVQTSTNLASVKMKGDKKDEADDKEIPLLKDNEIKIATSQRSSIESAKQDM
                     VDMVDCAFQHKGVKISHAYGYPGWKPNLNSKMLEASKKSYKKLYGSDPLVKAIHAGLE
                     CGLIGEKYPGMDMISFGPTLMGVHAPGEKIEINTVQKCWDLLLEVLKSVK"
     gene            complement(2167..3381)
                     /locus_tag="PLI16_02270"
     CDS             complement(2167..3381)
                     /locus_tag="PLI16_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOH83413.1"
                     /translation="MKDNKILDITADVKWIGVLDPDIRTFDIVMETKYGATYNSYFIN
                     AQKKTVVEATKEKFWDVYLEKLKQVVNPEEIEYIVMNHTEPDHSGNAINLLKIAPNAK
                     IVGTGNVIRYMTDLMNMDFPHIIVKEGDTLDLGNKTLRFINAPNLHWPDSMYSYLEED
                     KVLFTCDSFGSHYCHEEMYDDKVGNFDEAFKYYFDVILKPFSKYMLKAIEKIRPLDIS
                     AICNGHGPLLTKNWKRYVDLSEQYAKEAVALSEFGRALVAYVSAYGKTKILAESIAKG
                     LSQVEGIHADLCDIEKMPAGELADKIAQASGIIIGSCTINQNTLPQVYTMFALMSPLR
                     DKGKPAGCFGSYGWSGEAQKIIEANLSALKLNLVPDNIFVKFTPSADDITRCVEYGKS
                     FAAKVLEVKCGV"
     gene            complement(3402..6323)
                     /locus_tag="PLI16_02275"
     CDS             complement(3402..6323)
                     /locus_tag="PLI16_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOH83414.1"
                     /translation="MEDEILGKNENQDTPKEEGDTPQVFPKTQHLTGMYKNWFLDYAS
                     YVNLDRAVPDIRDGLKPVQRRILHSLKELDDGRFNKAANIIGNTMKYHPHGDASIGEA
                     LVILGQKDLLVETQGNWGNILTGDSAAAPRYIEARLSKFALEVVFNPKTTIWQSSYDG
                     RNREPVTLPVKFPLLLAQGVEGIGVGLSSKILPHNFIEIIDACIKHLRNEEFELLPDF
                     PTGGMIDVSKYNDGARGGKVRIRARISQEDKKTLVITEIPFGTTTVSLIDSIVAANDK
                     GKIKIKKIEDNTAAKVEIVIHLAPGVSPDTTIDALYAFTACETSISPICCIIDDQKPR
                     FITVHEILEDSVQHTLDLLQSELEIEKAECLEQLHFSTLERIFIEKEVYQLIRKSKTD
                     KEIDDTIDKGLKPYTKLFIRKVTIEDIHRLRKIPIERISKYNSDKAEDIINSLKDDIE
                     QVDYDLDHLVDYAITYYERLKMKYSKGRERKTEIKNFDVIQATMVAIANQKLYVNRQE
                     GFIGTGIKKDEYVCDCSDIDDILTFREDGTFMLSKVADKVFVGKNIIHVAVYKRFDER
                     TIYNMVYRDGKNGNAMVKRFAVTGVNRDKEYVLTKGTADSKVLYFTANPNGEAEIVNV
                     SLRPKPRLKILSFDFDFSTLAIKGRNSIGNILSKNPVRKITLKDSGISTLSERDVWFD
                     ESVNRLNTEQRGKHLGSFAPEDKIITFMKSGHFRIYNNDLSIHFDEDLLLIEKYNPDK
                     VYTVIYFDGESGLTFVKRFTAEDSDKKQFFISETPGSQMIEFMPCKNPVVELTFELKN
                     ARSKPSEIINLVDFIGVKSFKAKGKRLSNNQILTITPVITEEPPEEPLEGTENIQMQD
                     DGPADATDEEKNEGNDAVNDDTDIEDGKEAGMDSQTDENNEVKDEEVQEKKPAVEPHE
                     KKTKTPKTIIEKASREETLPKEPEKPAPEATPPTTQKKGTKKTKGKNDFPGAIQIEFE
                     F"
     gene            complement(6337..8382)
                     /locus_tag="PLI16_02280"
     CDS             complement(6337..8382)
                     /locus_tag="PLI16_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOH83415.1"
                     /translation="MSFLNPYFLFGLLAIAIPVIIHLFNFRRYKKVFFTNVRFLKEVQ
                     QETKKKSQLRHLIILALRILAIASLVFAFAQPYIPLAQNTTNPKAVNYISIYLDNSLS
                     MQAEGGRGSLLEEAKAKASEIISAYRSADHFQLLTNDFEARHRQFVTREEFVAMLREV
                     EWSPVARSLPEVAKRQAESFSEISGASKIAYVISDFQQSTSQLALFDPDTALAYYMVP
                     VKSNKSDNLSIDSCWFESPVHLLGQKVKLFVSVKNNAATIAEKVPLKLSVNNTQKAVS
                     SFDIAPGSSAGTIITYVDKEPGIHHGLIEITDSPVTFDDKFYFSYEIKNSVPVLCING
                     LQSNVFLNSLLAEDSAFSFVNVSVNQVDYNSLASYNLIILNEIPEVSSGLSEELFKFV
                     TNGGHLLIAPDEKMNIRNYQSFLTVLNSNYYTVLNKRPQKVAELNWQHPIYSDVFESV
                     PQNIDLPQVTTFFSMSRISRSAQDYLMKLQNGEPFLSVQDCGNGKVFLLSVPLKAECS
                     NFPKHALFVPTIYKIAMMSRMQPRLYYVIGSHQPVEVPTVPGGEKDMVLKLKNTGGND
                     EIIPQLQNQNGHITLLLNDQVRTHGNYILSNSGPVAGLAFNYSKDESDMKCYTPEEIT
                     AFIDKNTMENTMMLEGKAKSLAAVVSELNQGKKLWKTFVLLALLFLASEVVLLRIWK"
     gene            complement(8648..>9036)
                     /locus_tag="PLI16_02285"
     CDS             complement(8648..>9036)
                     /locus_tag="PLI16_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH83416.1"
                     /translation="GQADLIISVEPMESLRYIPMLSPDGWLITNSEPFINIPNYPEIG
                     KVHDAIRSFPRQVLMDADKMAKEINAAKSANMVILGAASPYLGIEYEKLEKAIRTIFG
                     KKGEEVVQTNLKAMALGKEFAVANVK"
BASE COUNT         2240 a         1964 c         2053 g         2779 t
ORIGIN      
        1 cagaaaagtt gttgctgccg cggccatcaa tgaaacctgg ctcaatgagc ttatatggcg
       61 ggaatttttt atgatgatac tatggcattt tccgcaggta gttacagaga gtttcaagaa
      121 aaaatacgac gatatctcat ggcgaaacaa tgaggaggaa tttgcccggt ggtgccgtgg
      181 tgaaacagga ttccccatag tggatgccgg tatgcgggaa ctccatgcaa caggctggat
      241 gcataacagg gtacgcatga ttaccgcagg atttttggtg aagcatcttt tgacagactg
      301 gcgatgggga gaggcttatt ttgcacagaa gctgatagat tacgaactgg cttcaaacaa
      361 cggcaactgg cagtgggcag cgggcagcgg ctgcgatgca gcgccctatt tccgtatctt
      421 taacccggca gaacaaacca gaaggtttga tccgcaacat gaatatataa aaaggtgggt
      481 gccggaatat actacaaaaa gttatgccca accgttggtt gagcataact ttgcgaggga
      541 acgtgccctc aatacataca aaaggtcttt attacgataa agactattta acgcttttca
      601 gcacctcgag cagcaggtcc cagcatttct ggacggtatt gatctcaatc ttttctccgg
      661 gggcatgcac acccatcaaa gtcgggccaa atgaaatcat gtccataccg ggatatttct
      721 cgccgatgag tccgcattca aggccggcat gtattgcctt taccagagga tcggagccat
      781 aaagcttctt ataagatttt ttggacgcct ccagcatttt agagttcaga ttgggtttcc
      841 agccgggata accataagca tgggagattt tcactccttt gtgctggaat gcgcaatcta
      901 ccatatccac catatcctgt ttggccgatt cgatagaact gcgctggctg gtggcaatct
      961 tgatctcatt gtcttttaat aagggtattt ccttatcgtc ggcttcatct tttttgtcgc
     1021 ctttcatttt tacagaagcc aggttagtgg aggtttgcac caaaccctga atatccggtg
     1081 acatagtgat aacgccgtgg ggacatgcat acaggcaatt gagcagtgtt tgctgggttt
     1141 ttatatcaag cacctgggca ggttttttgg ctcccgtgca ggtaatggtc agttggggtt
     1201 cgttagtttg gtattcgtct ttaatgttcc tggccacctc attgaaatac gcggcaaagg
     1261 ccgtggtatt ttttttggga attacgacta tgcattttcc ttcgcgggct atagcattgc
     1321 gcaggtttcc tgctttgata tatgatattc ttgcatcgaa tttttttgaa atattccaca
     1381 gaatgcgatt cagcaatttc acggcattac ccctgttttt gttgatgtct tcgccggaat
     1441 ggcctccctg caaatcgccg acattgatga ggaaagcctc atgagctgcc ggaacgggtt
     1501 cctttttgta caggaatctt gccaccgtgt cgccgccgcc ggcacatccg atagtaaatt
     1561 cgccttcttc ttccgagtcc agattgatga gcatacggct ttttaagaag tcgggtttca
     1621 gcgagaaagc gcctttaagg ccggtttctt catcaaccgt aaacaggcat tcgatgggac
     1681 catgctcgat ggaaatatcg gccaatatag ccatctgcat ggcaacgccg ataccattgt
     1741 cggcgcccag ggtggtttct tttgctatca gccagccttt ttcaacaacg gccttgattg
     1801 ggtccttttc gaaatcatga gtaatgttgc tgtttttctc acaaaccata tccacatggc
     1861 tttgcaacac aacccaggga agtttctctt tgcccttggt ggccggtttg cggataagca
     1921 cattgcccac ttcatcgaca agggtttcca gtttatgttt ctgaccaaat gctttcaggt
     1981 aagctgtaat tttttgctct ttgcccgaag gcctcggtat ctgcattatt tcatcgaaat
     2041 agccccatac agacttgggt ttcagtttta aaatttcctg gttcatatta ttaaaattta
     2101 aggattaatt aatatttgag aaaggaacat ttcgtgaaat gtcctaacag caacttcata
     2161 taatcattat acaccgcatt tcacttcaag cactttcgct gcgaagcttt ttccgtattc
     2221 cacacagcgt gtgatatcat cggcggaggg ggtaaattta acaaaaatat tgtcggggac
     2281 gaggttaagt ttcagtgcag agaggttggc ttcaataatc ttttgcgctt caccgctcca
     2341 gccgtaggag ccaaagcatc cggcaggttt gcctttgtct cgcagggggc tcatgagggc
     2401 aaacatggtg tatacctggg gcagcgtatt ttgattgatg gtgcagctac cgataatgat
     2461 gccgctggct tgtgcaattt tgtcggccag ctcaccggcg ggcatttttt caatatcaca
     2521 caagtcggca tgtatgcctt caacctgcga gaggcctttg gcgatgcttt cagcaagaat
     2581 tttagttttg ccataggcag aaacataagc taccagcgcc ctgccaaatt cagagagagc
     2641 caccgcttcc tttgcatatt gttcagacag gtcaacgtag cgtttccagt ttttcgttag
     2701 caaggggcca tgtccgttgc aaatagccga gatatcgagc ggccttattt tttcgatagc
     2761 tttcagcata tacttgctga aaggtttcag gatgacatca aaataatatt tgaaagcctc
     2821 atcaaaattg ccgactttgt catcgtacat ctcttcgtgg caatagtgtg aaccgaatga
     2881 atcgcaggta aataagactt tatcttcttc cagataggaa tacatagaat cgggccagtg
     2941 caggttcggt gcattgataa accgcagggt tttgttgcca aggtcgaggg tgtcgccctc
     3001 ttttactatg atatgcggga agtccatatt catcaggtcg gtcatatacc ggatgacatt
     3061 acctgtaccg acaatcttag cattgggagc aattttcagc agattgatgg cgttgcccga
     3121 atgatcgggt tcggtgtgat tcatgacgat gtattcaatt tcctccggat tgaccacttg
     3181 tttaagcttt tcaagatata catcccagaa tttttctttg gtagcttcaa cgacagtctt
     3241 tttttgtgca ttaataaaat aggagttata agtagctcca tatttggttt ccatcacgat
     3301 atcgaaagtg cggatatcgg ggtcaagcac acctatccat ttcacgtcgg cggtaatgtc
     3361 caatattttg ttatccttca taaaaagtaa aattaatgat tttaaaattc aaattctatc
     3421 tgtatggcgc ctggaaaatc gttttttcct ttagttttct ttgttccttt cttttgtgtg
     3481 gtgggaggag ttgcttccgg agccggtttt tccggttctt tgggcagtgt ttcttccctg
     3541 cttgccttct caattatggt tttcggggtt ttggtttttt tctcatgagg ttcaacggca
     3601 ggcttttttt cctgaacttc ttcgtctttg acctcattat tttcgtcggt ttgagaatcc
     3661 atacctgcct ctttgccgtc ttctatgtcc gtatcatcat ttacggcgtc attaccttca
     3721 tttttttctt cgtccgtggc atccgccggc ccgtcgtcct gcatctggat gttttcggtg
     3781 ccttccaatg gttcttcggg aggttcttcg gtgatgacag gtgtgatagt gagtatttgg
     3841 ttgttcgaca gccgttttcc cttggcttta aaacttttta caccgataaa atcaaccagg
     3901 ttgataattt cagaaggttt actgcgtgca tttttcaatt cgaaagtcag ctctaccaca
     3961 gggtttttgc agggcataaa ttcaatcatc tgagaacctg gggtttcgct gatgaagaac
     4021 tgttttttat cagaatcttc tgctgtgaaa cgttttacga acgtcaagcc cgactcgccg
     4081 tcaaaataaa tcacagtgta caccttatca ggattgtatt tctcaatcag gaggagatcc
     4141 tcatcaaaat gtatggataa atcgttgtta taaatcctga aatgccctga cttcatgaag
     4201 gtaataatct tatcctccgg tgcgaaggag cccaggtgtt tgccgcgttg ttcggtattc
     4261 aggcggttca cactttcgtc gaaccacacg tccctttcgc tcagggtgga aatgccggaa
     4321 tctttgaggg tgatttttct caccgggttc tttgacagaa tgtttccgat agagtttcgc
     4381 cctttgatag ccagcgtact aaaatcaaag tcgaaggata aaattttaag gcgcggtttt
     4441 ggccgcaggc tcacattgac tatttcggcc tcaccattgg ggttggctgt aaaatacaac
     4501 actttggagt cggcggttcc tttggtaagc acatattcct tgtcgcggtt gacgccggta
     4561 accgcaaagc gttttaccat ggcattgcca tttttgccat cgcggtacac catattataa
     4621 atggtacgtt catcaaaacg cttataaaca gccacatgaa tgatgttttt ccccacgaag
     4681 accttgtcgg ccaccttgga tagcataaat gttccgtctt ccctgaacgt gagaatatca
     4741 tcgatgtcgg aacaatcgca tacatattcg tctttcttaa tgccagtacc aatgaatcct
     4801 tcctgcctgt tgacatacag tttctggttg gcgatggcta ccattgtggc ctgtatgaca
     4861 tcaaaattct ttatttctgt tttgcgctcc ctgcctttgc tgtatttcat tttcaggcgt
     4921 tcgtagtagg tgatagcgta atcaaccagg tggtcaaggt cgtaatccac ctgttctata
     4981 tcgtctttta aagagttgat gatatcttcg gctttatcgg aattgtattt ggatattctt
     5041 tcgatcggaa ttttgcgcaa gcggtgtata tcctcgatgg taacttttcg gataaagagt
     5101 ttggtgtaag gtttcaggcc tttatcaatg gtatcatcaa tttctttatc ggttttcgat
     5161 tttcgtatca gttgatatac ttctttttcg ataaagatgc gttcgagcgt ggaaaaatgc
     5221 aactgctcca ggcattcggc cttttctatt tcgagttcgc tttgcaggag gtcgagcgta
     5281 tgctgaacgg aatcttccag aatttcgtga acggtgataa acctgggttt ctgatcatcg
     5341 ataatacaac agatgggtga gatgctggtt tcgcaggctg taaaggcata cagggcgtcg
     5401 atggtggtat cgggtgatac tccgggcgcc agatgaatga cgatctccac ctttgctgcg
     5461 gtattatctt caattttctt gattttaatc ttgcccttgt cgttggctgc cacaatacta
     5521 tcgataagcg acacggtggt ggtgccaaaa ggtatttcgg taataaccag cgtttttttg
     5581 tcttcctgac ttattcgggc cctgattctc accttgcctc ccctggcccc atcgttatat
     5641 ttggatacat ctatcatgcc gccggtagga aaatcaggca ggagttcaaa ttcttcgttt
     5701 ctgaggtgtt tgatgcaggc gtcgatgatt tcgatgaaat tatgaggtaa aatttttgat
     5761 gacaggccaa cgcctatacc ctcaacgcct tgtgccagga gtaacggaaa ctttaccggt
     5821 aaggtaacgg gttcacggtt tcggccgtca taagacgact gccagatagt ggttttagga
     5881 ttaaaaacca cttcaagggc aaatttcgag aggcgtgctt cgatataacg tggtgcggct
     5941 gcgctgtcgc ctgtaaggat attgccccag tttccctgcg tttccaccag caggtccttt
     6001 tgtccgagaa ttaccaaagc ttcgccgatg gaagcatccc catgggggtg atatttcatg
     6061 gtattgccga tgatattggc ggctttgttg aagcgcccgt catccagctc tttgagcgaa
     6121 tgcagtatgc gtcgctgcac gggtttaagc ccgtcgcgga tgtcgggcac ggcacgatcc
     6181 aggttaacat acgatgcgta atccagaaac cagtttttgt acatgcccgt aaggtgctgc
     6241 gttttgggaa acacctgcgg ggtgtcgcct tcctctttgg gggtatcctg attttcgttc
     6301 tttcctaaaa tttcgtcttc cataatttac tgcctgtcat ttccatatac gcaaaagtac
     6361 aacttctgaa gcaagaaaca gtagagccaa caatacaaaa gttttccaca attttttccc
     6421 ctgattcagt tcgctcacta ctgccgcaag agatttggct tttccttcca gcatcatagt
     6481 gttttccatg gtatttttat caataaaagc ggtaatctcc tccggagtat aacacttcat
     6541 atcggattcg tctttgctgt aattaaaggc caggcctgca acaggcccgc tgttagaaag
     6601 gatgtaattt ccatgggtgc gtacctgatc attcaaaagc agtgtgatgt gtccgttctg
     6661 gttttgcagt tgagggatta tctcatcatt tccacctgta ttttttagtt ttaaaaccat
     6721 atctttttca ccacccggca cggtaggcac ctccaccggc tgatggctgc ctatgacata
     6781 atataaccgc ggctgcatcc ggctcatcat agctatttta tagatggtgg gcacaaacaa
     6841 agcatgtttg ggaaaattgc tacattcggc tttgaggggc acagataaca ggaatacctt
     6901 tccgttgccg caatcctgaa cgcttaaaaa tggctcccca ttctgtagtt tcatcaggta
     6961 atcctgggcc gacctgctga tacggctcat ggaaaaaaat gttgtaacct gtggaagatc
     7021 aatattttga ggcacactct caaagacatc ggaatatata ggatgctgcc agtttaattc
     7081 tgcaactttt tgtggccgct tattcagcac cgtgtaataa ttggagttga gcacggtcaa
     7141 aaaactctgg taattgcgta tattcatttt ttcgtccggc gctatcagca aatgccctcc
     7201 attggtaaca aatttgaaaa gttcttcgga caaaccggaa gacacttctg ggatttcgtt
     7261 gagaatgatt aggttataag aagccaggga gttataatcc acctgattta ctgatacgtt
     7321 tacaaagcta aaagccgaat cctccgccag cagtgagttt agaaagacat tggattgcag
     7381 tccgttaata cacaatacgg ggacagaatt ttttatttcg tacgaaaaat aaaacttatc
     7441 gtcaaaagta accgggctgt cggtaatctc aataaggccg tgatgtatgc cgggctcttt
     7501 atcaacataa gtaatgatgg tacctgctga gcttcccggg gcaatgtcga agctgctgac
     7561 cgccttttga gtgttgttga cagaaagttt gagcggcact ttttcagcga tggtagcggc
     7621 attatttttc acagaaacaa agagtttaac tttttgcccc agcaggtgca cgggcgattc
     7681 gaaccagcag gaatcgatac tcaggttgtc ggatttattt gattttaccg gaaccatgta
     7741 ataagccagg gcggtatcgg ggtcgaacag ggccagttgt gaagttgatt gttgaaaatc
     7801 ggaaattaca taggctattt tactggcacc gctgatttcg ctaaaactct cggcctgccg
     7861 tttggccacc tcgggcaggc tgcgggcaac cgggctccat tccacttcgc gtaacatagc
     7921 cacaaactct tccctggtaa caaactgccg gtgacgggct tcaaaatcgt tggtaagcaa
     7981 ctgaaaatga tcggcactgc ggtaggcaga aatgatttca gaggccttag ccttggcctc
     8041 ttcgagcagg cttcccctgc cgccttctgc ctgcatactc aacgaattat ccagataaat
     8101 actgatataa tttacggctt tagggttggt agtgttttgg gccagcggga tgtagggctg
     8161 cgcaaaagca aacaccagtg aagctatggc caaaatacgc aaggccagaa ttatcaaatg
     8221 gcgtagttgt gatttttttt ttgtttcctg ctgaacttct ttaagaaaac gtacattagt
     8281 aaagaaaact tttttgtatc gcctaaaatt aaacaggtgt ataataacag gaatagcaat
     8341 agccaacaag ccgaaaagaa aatatggatt aagaaaagac atgttatgaa taaaaggcaa
     8401 atttaaaaaa aataatgcag cgataaatta aaaaaaacca aataaaaaaa agcccctgaa
     8461 gggcttttct ttattgcttg ttagcctttt taaccttttt ttcttcgcgt ttttctttta
     8521 aggattttgt ggcttttttc ttttcttctt tttgttgctt tgatttttct ttgctcataa
     8581 tttttaagtt taaaaattaa cgtatgtaaa attaagaaac atttttattt ctgcaaaaaa
     8641 atctgattta tttcacattg gccacggcaa actctttgcc cagtgccatg gctttgaggt
     8701 tggtctgcac cacttcctcg cctttttttc caaagatggt gcgaatggct ttttcgagtt
     8761 tttcatattc tatgcccagg taaggtgaag cagcccccaa aatcaccata ttggccgatt
     8821 tggccgcatt gatttctttg gccattttgt cggcatccat caaaacctga cgcggaaacg
     8881 acctgatggc atcatgcact ttaccaatct caggataatt cggtatgttg ataaatggtt
     8941 ccgaattggt aatcagccac ccatcaggcg aaagcatggg aatgtaccgt agtgattcca
     9001 tgggctccac ggagataatg agatctgcct gtccct
//
LOCUS       DAOSFP010000044         8999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6590_length_8999_cov_3.936606, whole genome shotgun sequence.
ACCESSION   DAOSFP010000044 DAOSFP010000000
VERSION     DAOSFP010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8999
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6590_length_8999_cov_3.936606"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..862)
                     /locus_tag="PLI16_02290"
     CDS             complement(107..862)
                     /locus_tag="PLI16_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOH83417.1"
                     /translation="MPASPLFYCAVLITLFTTVSCRQSQRKYSADLSVNQSIKDSAWG
                     GIDTATPTQLLMISHGLIDIENIDTLFLLDIKYSGTDNFLKTDIYGDYNKAYLQPEVA
                     AKLLKAQDLLSEQHPGYRLIIFDAARSVSAQKMIWDALKMPAREKGKFASNPARGSLH
                     NFGAAVDLSIVDEKGNELDMGCGFDYFGPLAYPVMENKLLSEGKLTKAQVENRKLLRS
                     VMHQAGFFNIQTEWWHFNSCTRQVAKEKYKLIE"
     gene            complement(864..3191)
                     /locus_tag="PLI16_02295"
     CDS             complement(864..3191)
                     /locus_tag="PLI16_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH83418.1"
                     /translation="MNHKTFFYLICLFFLSGLIPSQAQNYAECIAIGDEAFEDGDFFT
                     ASSYYSSALWYYSTDIQLAYKCAEAYRFFNNYQQARRWYSYVINNDSKGNLPLAQLWL
                     AMMEKSLGNYTEALLQFRAYYNTNKNQAESFYIRKARAEILACQEAPNIIANKQNVLI
                     EHLNESAVNTEFSEFNAVQLSDTALVYSALRPLAQSDYDTYFPNAYISKIYLAKSTVT
                     GWAKTSEIDAKINDKESHNANICFSKDHNKVFFTRAKADNNKNLAAEIFSCENIDGKW
                     QKAKKLPDKINVDGYTSTHPCFVEYDDQSLLFFVSDRPGGFGQLDIWYSIIKDGQYQD
                     PINLGSIINTPGDEITPYYHDSTQTLYFSSDWHKGIGGLDIFYSRGKFNTWTFPVNIG
                     YPINSSCNDTYFTVNEVDNDGFFTSNRPGSLSIKSATCCYDIYSYEWQDTVKEKTVAV
                     VPIDTIGQDTVSIEENINLMLPLTLYFHNDEPDPNTLNKTTKRNYQTLLGEYYAMEDT
                     YIMEYAKGLSGHEKVKAEKDIKDFFENYVAQGFTQLKLFAELLHKDLKKGNSIKIKIK
                     GFCSPLNTTDYNLNLSMRRISSIVNYLKQYYEGILLEYINGTASNGAKLTILEEPLGK
                     STASPLVSDNPNDKRNSIYSRAAAFERKVQIILYEHEKQNDADDQKIPDIQFEDNLFD
                     FGQITQGDEAVITIRFKSTGTAPLNIAGVETSCGCTLVDWPREPFEPGREGTLKISFN
                     TTEDPGEHNETVTIYTNTKKAKYIIPVHALLLPKN"
     gene            complement(3197..4231)
                     /locus_tag="PLI16_02300"
     CDS             complement(3197..4231)
                     /locus_tag="PLI16_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOH83419.1"
                     /translation="MSLTTDSLKKVYCIIFFSVFFAGVFGQDVHYSQYYAAPLELNPA
                     QAGLIKGRMRVGLNSKRQWSSVTKPYQNVAAYFDMQAVRRKFRKDALGVGVLFDADIA
                     GDSKYSVIKAGCAVSYIKSLSYQNNNFIAVGIMPGIIQQSIDYTALYYDNQYNGNYYD
                     PAIDPGEQYGMRSFLNFDLGAGVHWFYQVNKTTQFSAGYAVGHLTKPRVGYMNDNSIR
                     LNMKHTLYGGSQFEASPDLDIIPGIMFSYQNPFAEVIFGTMLKYNRSKKYIDNTSINF
                     GLYGRYADALIVVAGMDYKNINFGVSYDINLSRLRPASKVRGGLEFSISYTYDKNRYR
                     RIREIPCPIF"
     gene            4341..4826
                     /locus_tag="PLI16_02305"
     CDS             4341..4826
                     /locus_tag="PLI16_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOH83420.1"
                     /translation="MIQRIQSLYLMLAVVAYALLFFFPIAEYNVLDATYYFTMFEIPR
                     GNSNSTLPFLIVVGLLALSCLITIFLFKKRPLQIKITAITLLVHIGLIAALFYSADNL
                     IAGSLTKWVTEQGAANSAIVPTYKAGMYISLIPIVFIVLAHKAIRKDERMVSSSDRLR
                     P"
     gene            complement(4823..5317)
                     /locus_tag="PLI16_02310"
     CDS             complement(4823..5317)
                     /locus_tag="PLI16_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOH83421.1"
                     /translation="MKRIGILSDTHCFLHEKIFDFFSSCDMIWHAGDIGNLETAERLE
                     NFKPLIAVYGNIDGQELRVRYPEKQLIKVEQHRIFITHIGGTPSKYDLKARTTIESLK
                     PTIFVCGHSHICKIQYDQRQNLLYINPGAAGKYGFHTKITLLRFVIDGNNLKDMEILE
                     LYKH"
     gene            complement(5327..5761)
                     /locus_tag="PLI16_02315"
     CDS             complement(5327..5761)
                     /locus_tag="PLI16_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83422.1"
                     /translation="MNSITRLIVFIAAILSTINLPAQEDTYHKNDSIINYQNLDSDEA
                     RQAWAAMHETWLTCCFYDCLKQNNIALSCARCEKVMLTVDFKIDSNGRATEYRIIKEN
                     RCGKPFSEKLKQCFLEYFLEATFDETLRNIVLEISIGNGLKC"
     gene            5882..6352
                     /locus_tag="PLI16_02320"
     CDS             5882..6352
                     /locus_tag="PLI16_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HOH83423.1"
                     /translation="MKYIVKQKLWSLTDQFSVKDQQDNVVFFVDSERFSVAKKLFLSD
                     NKKNVLFRIEQKLMKLLPIYEIYKGDMLYAVLKKKPGFLKAKIEIEQNGAPLLISGDK
                     LSLDFTISSDGKELAKISKKWSGLSDTYAVDISTGENDAFVIAMVICIDEMIYE"
     gene            6407..7789
                     /locus_tag="PLI16_02325"
     CDS             6407..7789
                     /locus_tag="PLI16_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83424.1"
                     /translation="MLFIFLFIYLVLGLLTIFFFDGTGDAGDSVMHYLLARYAPRHPE
                     LYFNHWAKPVYVLLVSPFAQLGFHGVKLFNLLAMMLTIILTCLSARILKINNSSVVPL
                     FIIFAPYVYAQTFSGLTEPLFALFLAAGIYLIIKDKFVPAAIVISFLPFVRSEGLIII
                     GVFALYFIYRKKWSKLLWLMCGHVCYSLAGYFVNHDLLWVFRKIPYASLASPYGSGST
                     FHFVNQLFYVLGVPVFILFCIGLLSYFPRLNNKNQDKNAENFFLILGCAIAFIAAHSI
                     FWKFGIFNSMGLKRVLIGIMPALVIIALQGFNLISEKFIAPRPVQLAVQLVTVAYILS
                     FPFTGNPAALNIKKELLPDKGQQSAREVTAYINKNFSSHGKLFYAYPYFSETFDIDHF
                     DKSKHLELNRDALNSLAQGDLVLWDDWFASQTLEISPEYLLNFPGMKKIKEFYAEDAS
                     SATFILFKYK"
     gene            7872..8732
                     /locus_tag="PLI16_02330"
     CDS             7872..8732
                     /locus_tag="PLI16_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83425.1"
                     /translation="MKKIIILIIIVFGWYSADAQSNIQKVAAQSQAAPNIATSTAGQQ
                     TTVTSAQPGNYNQTSGNNAPAYTTSSRQVNSNQYISVSQSFSRGRNSGRSRIYVKSPG
                     RNFALNAAYLVPAIKTTLVSACSINNRVAAAPMNFSSYYQCDTVHNSGILPEIRPVSK
                     GESGVGIVTNRMQSFSAAINNHRPQYRCLNNNFMENCGMGWYSIHLASFKNLDYCRAA
                     IRYIKSRYKTDVFLFEDFSVYNRYHLVMGKYRKYFVAENMLRKIRKEMPCAFVVNWDN
                     YYQRILFTQE"
     gene            8810..>8999
                     /locus_tag="PLI16_02335"
     CDS             8810..>8999
                     /locus_tag="PLI16_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83426.1"
                     /translation="MRKIIFFSVLCACILVFAACEKCYDCTKKCGICTKEGFETLAGC
                     QGDSALNGYSVEAWKLHLE"
BASE COUNT         2417 a         1819 c         1913 g         2850 t
ORIGIN      
        1 gcggccttat cggttacaat tatttcaccg tcccgcatta ccccatgcag gatcgtttat
       61 tccggtttgg cattagctgg cgttttcacg attagtggaa taacaatcat tcaattagtt
      121 tgtacttctc ctttgcaacc tgccttgtac acgaattaaa atgccaccac tcagtctgga
      181 tattaaaaaa tccggcctgg tgcatcactg atcttaataa tttgcggttt tcaacctggg
      241 ctttggttag tttcccttcg gatagaagct tgttttccat tacaggatag gcgaggggcc
      301 caaaataatc gaaaccgcaa cccatgtcga gttcgttacc tttttcgtca acgatgctca
      361 ggtccactgc cgccccgaaa ttatgcagcg aacctcttgc ggggttactg gcaaatttcc
      421 ctttttcgcg ggcaggcatt ttcagcgcat cccatatcat tttctgggca gaaacacttc
      481 gtgcggcatc aaaaataatc aaccggtatc cgggatgctg ctcgcttaac agatcttgtg
      541 ctttcaataa ttttgcggct acttccggct gtaaataagc tttattataa tcaccataga
      601 tgtctgtttt caggaaattg tccgtgccgg aatacttgat atccaaaaga aacagggtgt
      661 caatgttctc aatatcaatc aggccgtggc ttatcattaa taattgtgta ggtgtcgccg
      721 tatcaattcc gccccatgca ctatctttaa tggattggtt aacagataaa tccgctgagt
      781 attttctctg tgattgccgg cacgataccg ttgtgaacag ggttatcagc acagcacaat
      841 aaaaaagcgg tgaggctggc aaattagttt ttaggcaaca gtaaagcatg cacgggtatg
      901 atgtatttag cttttttagt gttggtgtaa atggtaacgg tttcgttgtg ttcgccagga
      961 tcttcggtgg tattgaagga aattttcaat gtgccttccc tgcccggttc aaagggttct
     1021 ctcggccagt ccaccagagt acatccgcaa cttgtttcta ccccggcgat attcagggga
     1081 gctgtgccgg tacttttaaa acggatggtg ataacggctt cgtcgccttg tgttatttgg
     1141 ccaaaatcaa aaaggttatc ctcaaactgt atatctggta ttttttggtc atcagcatca
     1201 ttttgttttt cgtgttcgta aaggataatc tgtactttac gttcaaaagc ggctgcacgg
     1261 ctgtaaatgg aattacgctt gtcgttgggg ttatcactca ccagcgggct ggcagtggat
     1321 tttcctaaag gctcttccag aatggtgagc ttggcgccgt ttgaagccgt accgttgatg
     1381 tattcaagca aaataccttc ataatattgt tttaggtagt ttacgataga ggaaatgcgt
     1441 ctcatcgaaa ggtttagatt gtaatcggtg gtgttcagcg gactacagaa tcctttgatt
     1501 ttaatcttaa tactatttcc ctttttaagg tccttatgca ggagttcggc aaacaatttc
     1561 agttgtgtaa agccctgggc aacataattt tcaaaaaaat ctttaatatc tttttctgcc
     1621 tttacttttt catggcccga caatcctttg gcatattcca tgatgtaagt atcctccatc
     1681 gcataatact cgcccagcag ggtctgatag tttcttttgg tggttttgtt cagtgtattc
     1741 gggtcaggtt cgtcgttatg aaagtataaa gtaagcggca acatcaggtt aatattctct
     1801 tcaatgctga cggtatcttg ccctatcgtg tctattggca ctacagctac ggttttttct
     1861 tttactgtgt cttgccattc ataagaatag atatcataac agcaggttgc actttttatc
     1921 gataaggatc ccggccggtt ggaagtaaaa aagccatcgt tatccacttc gtttacagta
     1981 aaatacgtgt cattgcaact gctgtttatc gggtaaccga tattaaccgg aaaagtccag
     2041 gtgttaaatt ttcctctgga atagaaaata tcgagtccgc cgatgccttt atgccagtcg
     2101 gagctgaaat acaaggtctg tgttgaatca tgataatatg gtgtgatttc atcgcccgga
     2161 gtgttgataa tgctgccgag gttgatagga tcctggtatt gcccgtcttt gatgatactg
     2221 taccaaatgt cgagttggcc gaatccgccg ggtctgtccg aaacgaaaaa cagcaggctt
     2281 tgatcatcat attcaacaaa acagggatgt gtagaggtat atccgtcgac atttatttta
     2341 tcaggcagtt tttttgcttt ttgccatttc ccatcgatat tttcacagga aaaaatctct
     2401 gctgccaggt ttttattgtt gtcggcttta gcccgggtga aaaaaacttt gttgtgatct
     2461 ttagaaaaac agatattggc gttatggctt tctttgtcat tgattttggc atctatttct
     2521 gatgttttgg cccatccggt aacggtggat ttggccaggt aaatctttga aatgtatgca
     2581 ttagggaagt aggtgtcgta atcgctctga gccagtggcc tcagggccga atataccagg
     2641 gctgtatcgc tgagctgaac agcgttgaac tccgaaaatt cggtgtttac cgcactttca
     2701 ttaagatgtt cgatgagcac gttttgtttg ttggcgatga tattgggagc ctcctggcat
     2761 gccagaattt ctgctctggc tttgcgtata taaaaacttt cggcctggtt tttattggtg
     2821 ttataataag ctctgaactg tagcaaagcc tctgtatagt taccaagcga tttctccatc
     2881 atggcgagcc aaagctgagc caggggcaaa ttccccttgc tgtcgttgtt gataacatag
     2941 ctgtaccaac gccgggcttg ctgataattg ttgaaaaacc ggtaggcctc ggcacatttg
     3001 taggccagct ggatgtcggt ggagtaatac cataaagcgc tgctgtaata ggaggaagcc
     3061 gtaaaaaaat caccatcttc aaaggcttca tcaccaatag caatacactc ggcatagttc
     3121 tgcgcctgtg atggaatcaa ccctgataaa aaaaacaaac atatgaggta aaaaaaagtc
     3181 ttgtgattca tcttttttaa aaaattggac atggtatttc tctgattctt ctgtacctgt
     3241 ttttatcgta tgtatagctg atggaaaatt ctaatccgcc cctgaccttg ctggctggcc
     3301 gcagccgcga taagttgatg tcataactta ccccgaaatt tatattctta taatccattc
     3361 cggccaccac aatcagcgca tcggcgtatc gcccgtacaa accaaagtta attgaggtgt
     3421 tgtcaatata ttttttgctg cggttatatt ttagcatagt gccaaatatc acttcggcaa
     3481 agggattctg ataactgaac atgatccccg gaatgatgtc cagatcgggg ctggcctcaa
     3541 attgcgaacc gccataaagc gtgtgtttca tgttgaggcg tatgctgttg tcgttcatgt
     3601 agcctactct gggcttcgtg aggtgaccaa cagcatagcc ggccgaaaac tgggtggttt
     3661 tgttaacctg gtaaaaccaa tggacaccgg cccccaggtc aaaatttaaa aaactgcgca
     3721 tgccgtattg ctcgccgggg tcaatagcgg ggtcataata attgccgttg tattgattgt
     3781 cgtaataaag cgccgtgtag tcgatacttt gctggataat tccgggcatg atgccgacag
     3841 caataaaatt attgttttga tacgaaagtg atttgatgta actaacggcg cagccagcct
     3901 ttatcaccga atatttggag tcgccggcaa tgtcggcatc aaacagcacc ccaactccca
     3961 gagcgtcttt gcgaaatttg cgcctgacgg cctgcatgtc gaaataggct gcaacattct
     4021 ggtaaggttt ggttaccgag ctccactgcc ttttcgaatt aagtcccacc cgcatacggc
     4081 ctttgattaa tccggcctgt gcagggttaa gctcaagcgg tgccgcataa tattgagagt
     4141 aatgcacatc ctgcccgaaa acaccggcaa aaaaaacaga aaagaatatg atgcaataaa
     4201 cttttttcaa cgaatcagtg gttaatgaca tacgcctttg ctgatgtaaa aataataatt
     4261 ataacttgaa aaaatacgga ttacctaaaa ttaacccgaa aggtaatcga ctaattgttt
     4321 atttttgtaa aaaaattatt atgatacagc gtattcaaag tctttatctg atgctggctg
     4381 tagtggctta cgcgttgctg tttttctttc ctatagccga atataatgtg ttggatgcca
     4441 cttattattt taccatgttt gaaattccca ggggcaacag caacagcacc ctgccttttc
     4501 tcatagtggt tggcttgctg gccctgagtt gcctgataac tatctttctg tttaaaaaac
     4561 gccctctcca gataaaaatt acggccatta cactgttggt gcacataggg ttgattgccg
     4621 ccctgtttta ttcagctgat aaccttattg ccggctccct cacaaaatgg gttaccgaac
     4681 agggtgctgc aaattcagct attgtgccca cctacaaagc gggtatgtat atctcactga
     4741 tacccattgt atttatcgtt ttggcgcaca aagccatccg caaagatgaa cggatggtaa
     4801 gctcttccga ccgtttgcgg ccctagtgtt tatatagttc aagtatttcc atatctttta
     4861 aattgttacc gtcaatcaca aaacgcagca gggtgatttt tgtgtgaaaa ccgtattttc
     4921 cggctgctcc cggattgata tacaacaaat tctgccgctg gtcgtattga attttgcaga
     4981 tatgcgaatg tccgcagaca aaaatggtgg gttttaatga ctcgatggta gtccgggcct
     5041 taaggtcata ttttgagggc gtgccgccaa tatgggtgat aaaaatcctg tgctgttcaa
     5101 ctttaataag ttgtttctcc ggataccgta ccctgagctc ttgtccatcg atgttgccgt
     5161 aaacggctat aaggggtttg aagttttcga ggcgttccgc agtttccaga ttcccgatat
     5221 cacctgcatg ccatatcata tcgcaggagg aaaaaaaatc aaaaattttc tcgtgaagaa
     5281 aacaatgggt gtcagataag atgcctatgc gtttcatgaa acaaaactaa cattttaacc
     5341 cgttgccaat cgaaatttct aacacgatat ttcttaatgt ttcgtcgaaa gtggcttcca
     5401 gaaaatactc cagaaaacat tgcttcaact tttctgaaaa gggcttgccg cagcggtttt
     5461 ctttaataat cctgtattcg gtggccctgc cattgctgtc aattttaaaa tccacggtaa
     5521 gcatcacttt ttcgcatcgg gcacaactta aggcgatgtt gttttgtttc aggcagtcgt
     5581 aaaaacagca tgtaagccag gtttcgtgca ttgctgccca tgcctggcgg gcctcatccg
     5641 aatccaggtt ttgataattg attatggaat cgtttttatg ataagtgtct tcctgtgccg
     5701 gcaggttgat agtgctgaga attgcagcaa taaatactat tagtcttgtg atgctattca
     5761 tcgcttgtgt ttttatttca ggtttatgca tttaccatcg ccttttttcg ggagttgggg
     5821 tagttttcta accgtttcaa tagggaatat agtgtacatt tgcactataa aagtacaaaa
     5881 aatgaagtac atagtaaaac aaaaattatg gtcgctgaca gatcagttta gtgttaagga
     5941 tcagcaggat aatgttgttt tttttgtaga ttcggaacgt ttttcggtgg caaaaaaatt
     6001 atttttatcg gataacaaaa agaatgtttt gtttagaatt gaacaaaaac taatgaaatt
     6061 actgcccatt tatgagattt ataaagggga tatgttgtat gcggtcttga aaaagaaacc
     6121 cggtttcctg aaagcaaaaa tcgaaattga gcaaaacggc gctcccttat tgatttcggg
     6181 tgataaactg tcgctggatt tcaccatatc atcagatgga aaagaactgg cgaagatctc
     6241 aaaaaaatgg tcgggactaa gcgatactta tgccgttgat atttcaaccg gcgaaaacga
     6301 tgcctttgta atcgccatgg tgatttgtat cgacgagatg atttatgaat aaatagagat
     6361 atcgtattgt aaaacaatag tttatacaat gttcaaaaaa tcttatttgc ttttcatttt
     6421 cttgtttatc tatctggttt taggcctgtt gacgatattt tttttcgatg gcaccggcga
     6481 tgcaggcgac agcgtgatgc actacctgct ggcacgttat gctccaagac atcctgaact
     6541 atattttaat cactgggcca agcctgtgta cgttttattg gtctctcctt tcgcgcaatt
     6601 gggttttcat ggtgtcaaac tttttaatct gttggccatg atgctcacaa taattcttac
     6661 ctgcctttcc gccaggattc tgaaaatcaa taatagcagt gtcgtcccct tgttcattat
     6721 ttttgctcct tatgtttatg cacaaacatt ttcgggactt accgagcctt tgtttgcact
     6781 ttttctggct gcaggtattt atctgataat caaagataag tttgtgcctg cagccattgt
     6841 tatttctttt ttgccctttg tacggtccga aggcttgata ataatcggtg tttttgcact
     6901 gtatttcatt tacagaaaaa aatggtcaaa actgctgtgg cttatgtgcg gacatgtgtg
     6961 ttattccctg gcaggatatt ttgtaaacca cgacctgctt tgggtttttc ggaaaattcc
     7021 ttatgccagc ttagcgagtc cttacggaag cggatccact tttcattttg taaatcagtt
     7081 gttttatgtg ctcggtgtcc cggtttttat tcttttctgc ataggcctgc tcagctattt
     7141 cccacgactg aataataaaa atcaggacaa aaacgctgaa aacttttttt tgattcttgg
     7201 atgtgccatt gcatttattg cggcacacag tattttctgg aaatttggaa ttttcaactc
     7261 tatgggactt aagcgggtgt tgataggcat aatgccggcg ctggtcatca ttgccctgca
     7321 gggtttcaac ctgatctcag aaaaattcat tgcaccacgg cctgtacaac tcgctgtgca
     7381 actggttact gtggcttata tactgtcttt tccttttacc ggaaatccgg cagccctcaa
     7441 tatcaaaaaa gaactgctac cagacaaagg tcagcaaagt gcccgggaag taacagccta
     7501 tattaataaa aatttttctt cccatggtaa gctgttttat gcttacccct acttttcaga
     7561 gacatttgat attgatcatt ttgataaatc aaagcatctt gaactaaacc gtgatgcgtt
     7621 aaattccctt gctcaaggcg atcttgtttt gtgggatgat tggtttgctt cacaaacatt
     7681 ggaaatttca ccagaatacc tgctgaattt tcccgggatg aaaaaaatta aagagtttta
     7741 tgccgaagat gcttcttcag ccacttttat tttatttaag tacaaataaa ttatatataa
     7801 agctcatctg gcttgatatt tgtggcaatt attttctgac tttttcattt ttgaaaccta
     7861 aaaaaaaacc gatgaaaaaa ataattatac taattattat cgtttttggc tggtatagtg
     7921 ctgatgcaca gtcgaatatt cagaaagtcg ctgcgcagag ccaggccgcc ccaaatattg
     7981 ctacttctac agccggacag caaacaacgg taacctctgc tcagcctggt aattataatc
     8041 aaacatccgg caataatgct cccgcttata ccacttcttc ccggcaggtt aattctaatc
     8101 agtatatttc tgtaagtcag tcgttcagcc ggggcagaaa ctccggtcgc agcaggattt
     8161 atgtaaaaag tccgggaagg aactttgctc ttaatgcagc ttatcttgta cctgccatca
     8221 aaaccacgct ggtttcagct tgttctataa ataacagggt agctgctgct ccaatgaatt
     8281 tctcgagtta ttatcaatgt gatactgttc ataattcggg catactcccc gaaataaggc
     8341 cggtatcaaa aggagaatcg ggcgttggca ttgttacaaa cagaatgcag tcattctcgg
     8401 cagcaattaa taatcataga ccgcaataca gatgtttaaa caacaatttt atggaaaatt
     8461 gtggcatggg ctggtatagc attcatcttg cctcgtttaa aaacctggat tattgtcgtg
     8521 ccgctatccg gtatattaaa agcagataca aaacggatgt ttttcttttt gaagattttt
     8581 ctgtttataa ccgctaccac cttgttatgg gaaaataccg gaaatatttt gtggcagaaa
     8641 atatgctgcg caaaatccgt aaagaaatgc cttgcgcttt tgtggtaaat tgggacaact
     8701 attatcaaag aatcctgttt actcaggaat gagccgcgga cagataaaag aaaaaaatca
     8761 ttcttttttg tttttgtgcg attttatgct aatattgtaa aaaaaaacca tgagaaaaat
     8821 aatattcttt tcagtacttt gtgcatgcat actggttttt gcggcctgtg aaaaatgtta
     8881 cgactgtacc aagaagtgtg gaatttgtac caaagagggt tttgaaactc ttgccggatg
     8941 ccagggcgat tctgcattaa acgggtactc ggttgaagcc tggaagttgc atttagaaa
//
LOCUS       DAOSFP010000045         8974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6616_length_8974_cov_3.804350, whole genome shotgun sequence.
ACCESSION   DAOSFP010000045 DAOSFP010000000
VERSION     DAOSFP010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6616_length_8974_cov_3.804350"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="PLI16_02340"
     CDS             <1..245
                     /locus_tag="PLI16_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83427.1"
                     /translation="DFFKDMLQKKDPQFLKETSTMMINWERETYPANIIHIHGSDDHV
                     LNIKNIKADYIIVNGTHIMTYERGREISEIIGKVLQ"
     gene            511..6708
                     /locus_tag="PLI16_02345"
     CDS             511..6708
                     /locus_tag="PLI16_02345"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOH83428.1"
                     /translation="MKTFKIIAFVMIVSVAAMAVKSFIPAFVHQKSITEMISINDDYK
                     KLWAKADSLVNKGLTRSAIEVVQVIYEKAKTENHTGNFVKAVTYKLKLESYISEDDYV
                     KEINDLTKEASEARFPIKPVIHSMIAEVYWKYYQNNRYKFLNRTETVDFIPEDIRTWD
                     LRKIVEQSIKYYQLSLQDEANLKKTSINVYDEILVKQGNDSKNFRPTLYDFLIHRAID
                     FFANEEPGLVNPVTRFEIDKEEYFSPFDNYVKVPITTSDNYSLTYYAVTLFQGLLDFH
                     KNDVNPEALIDDDLKRLDFIRQKSIHPDKDTLYLQALQALWKKFETFPSSSEVIYKIA
                     NYYYTEGNNYATANNEIDRWKLKKAYELCDIAIKKYPDAFGSKNCIALQKKIKERSLV
                     LTAENVNVPDQPFRGLLTYRNISKVHIRICKTNYEWFDKLTHEYYYDDKLIKALVKLT
                     PSKQFSVDLPDPKDYQGHSTEIKFPELEKGFYIILIGTDAGFTYNTEAVAYTDLWVSN
                     ISYMKRTLTSGNTQFVVLDRQNGQPMKGVSAQVFSEVYNYSTRRYEYHSGARYTSNED
                     GMVEVISTNNNYNNSFVEFTYNNDKLKSSDFYMYKAPKYANTKQNRTIFFTDRMIYRP
                     GQTVYFKGLLLETDGETSAIKPLTETNVILYDVNYQKVTETKLTSNEYGTFSGTFILP
                     TGSLNGQMHLADSWGSLYFSVEEYKRPKFEVKFEPVKGTYKLGEKVTVKGSAVAYAGS
                     NIDNAQVKYRVVRTVRFPYWWYYWYGYYPSSPQMEILNGSTVTNENGEFSIDFTAIPD
                     YKVSKKSDPTFTYQITADVTDINGETRSSQTIMNAGYKALLVNVDMPGEIEKSSKDTY
                     VISTTNLSGSYEYAKGNIVIHQLKQPEKLFRNRLWKEPDMKTISKNDYYLWFPYDQYE
                     NESNTSKWAKGSKLLENTFDTQKDSLLSLPNLSSWPSGQYVLEINSVDKFGEKVQSFS
                     YFTVYSNADKTIPVNDFAWFSTLKAKGEPGEEAAFLIGTKEQNVSVLMEVEVRNNIIQ
                     KQWIKLSNELRKISIPIKEEYRGNFAVHFSFVRHSRVFQYNVSVEVPYSNKELDITFE
                     TFRNKLLPGQNEEWKIKIKGKNGEKVAAEMLAGMYDASLDAYRPNSWFLSLYNHYYMQ
                     KYWDATGGFSSVGSSIYSVNWNPYVYFNYRAYDYLNWFGYDNYYYRYNRGYAYYADYD
                     GAGIDEAEINDISVTTLQSKNGRAMKKAEAAAPLAGATGHAEGDVAMANFETSATEQS
                     KDKASGGEKKDSRQDGLTDIKTRTNFNETAFFYPHLLTNENGEIVITFTVPEALTKWK
                     FMGLAHTKDLKFSQIQKEVLTQKDLMVMPNPPRFFRENDKIVFSAKITNLANDSITAD
                     ASLMLFDASTQKPIDLLMKNTDQVKCVGIPKGQSKAVEWTLSVPENIAAITYKVVAKA
                     GNFSDGEEMTIPVLTNRMLVTETLPLPIRGNQSKNFSLEKLINAYKSNTLKNYKLTLE
                     FTSNPSWYAVQALPYLIEYPYECTEQIFSRFYANSIATYIANSNPKIREVFDSWKNIT
                     PDALLSNLEKNQDLKYLMLQETPWVMEAKNESQSKRNIGVLLEAKRMQGEIEKALKKL
                     EKLQVPNGGWTWFPGMPDDRYITQHIITGFGHLDKLGVRDVRDQYKVWNMVKRGSRYL
                     DDRVREDYEWLLRYDPDHMFENHLGGIQIQYLYARSYFIKDIPIESKNKKAFDYYFGQ
                     AKKYWLPNNRYLQGMIALAMNRLNDKATATDIVKSLKENAIFNDEMGMYWKDMDYGYY
                     WYQAPIETQALLIECFDEVANDKQSVEDMKVWLLKQKQTQNWKTTKATAEACYALLLR
                     GTDWLARDPQVSISLGGMLVDPKNLPDAKQQAGTGYFRTSWFDEAIKPEMGKVKVSKT
                     DDGVAWGALYWQHFEQLDKITVAETPLKLGKKLFVERNSDHGPVITPVTEDTQLKLGD
                     KIKVRIELRVDRDMEYIHMKDMRASGFEPINVLSQYKYQGGLGYYESTGDAATNFFIS
                     FLKKGTYVFEYPLRVAQKGDFSNGVTTIQCMYAPEFSAHSEGIRVKVSG"
     gene            complement(6837..7415)
                     /locus_tag="PLI16_02350"
     CDS             complement(6837..7415)
                     /locus_tag="PLI16_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOH83429.1"
                     /translation="MKIDAIESGAVYHIFNRGNNKENLFIEEKNYKYFISLMEKYLAN
                     IADIYCYCLMKNHFHLLLKIKESTNIAEEKYRKKPHLAFSHFFNSYTKSINKAYNRTG
                     SLFQEHFHRNRITEDDFFKNVVAYIHLNPEKHGFVKDFRNYPHSSYADFFSERKSFID
                     KDFVINFFGNIANFEAWHCQKEWLLKGIIEDL"
     gene            7584..8171
                     /locus_tag="PLI16_02355"
     CDS             7584..8171
                     /locus_tag="PLI16_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83430.1"
                     /translation="MFAFGNKHPYHEVIISTQIKSNNPVALAIHDQRQYVVDGSKSAT
                     YCGNLRSGVGIPYDITTKSGKPFADDMLDVVGKALKKNGIKLSKVVTRSGLTKEAVIA
                     ELVKTQAPRSLYIRILEWKTDTYMKADLYCLVLLQVLDNNGNIVAENSVKTSREAFGG
                     SFWSPQKAAKKGALKALKMKMEALINEPNIIKAFE"
     gene            8220..8861
                     /locus_tag="PLI16_02360"
     CDS             8220..8861
                     /locus_tag="PLI16_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83431.1"
                     /translation="MAIIIIDCFACKAQESIIPIRCDSMYYVSILDSLKSSYPNCYNI
                     PPEFELAFYTAISHYPELKATNIEMKLKKFNFSMAARPAKKLLANRKKRIYRVYANIK
                     KDFTGLLPSRLNYNQRTGIIGHELGHILDYSNKNMCKMIGTGIGYMSRAYRRKLEAKT
                     DIEAIRHGLGWQLYDFNNYLYNYSDASEKYKKKKKRIYLDEKEIKELIINNSK"
BASE COUNT         3007 a         1834 c         1803 g         2330 t
ORIGIN      
        1 aagatttctt taaagatatg ctgcaaaaaa aagatccgca atttcttaaa gaaacctcga
       61 cgatgatgat aaactgggaa cgggaaacat accctgcaaa cataatacac atccatggca
      121 gcgacgatca tgtgctgaat ataaaaaaca tcaaggcaga ctacatcatt gtcaacggca
      181 ctcatataat gacctatgaa cggggcaggg agatatcgga aataattggt aaggtgctgc
      241 aataatataa ctaagagatc acagtgccta tttttgtctg aaaaaataaa aaaaatattt
      301 tttataagtc gccaaatgtg gtgtttttag ggcacatttg gcgggttata actaacaacc
      361 ccgattaaaa taaaattcca caggcaaatt gttgcccttt atccgatgaa ggggaagcta
      421 tcggatgaat tccttcaaaa aaaatttctt ttattttatg gattttttcc gatattgcat
      481 ctatcaaagc aaattcaaaa aatcttttct atgaaaactt ttaaaattat tgcctttgtc
      541 atgattgtgt cggtggctgc tatggcggta aaatcattca ttccggcctt cgttcatcag
      601 aaatccatta ccgaaatgat ttcaattaac gacgattata aaaaactatg ggcaaaagcg
      661 gattcgctgg tcaataaggg ccttacacgt tcggcaattg aagttgtaca ggtgatttac
      721 gaaaaagcca aaacagaaaa ccacaccgga aatttcgtga aagcagttac ctacaaactt
      781 aaacttgagt cgtacataag cgaggacgat tatgtaaaag agatcaacga cctcaccaaa
      841 gaagcttcag aggccagatt tcccatcaaa cctgttatac actcgatgat agcagaggtt
      901 tattggaagt actatcagaa caaccgctac aaatttctga accgcaccga aacagtggat
      961 tttatccctg aagacatcag aacatgggat ttaagaaaga tagtggaaca atcaataaaa
     1021 tattaccagc tttcattgca ggatgaagcc aatttaaaga aaacatccat caacgtgtat
     1081 gatgaaatac ttgtaaaaca aggcaacgat tcgaaaaatt tcaggcctac actttatgat
     1141 ttccttattc accgggctat tgattttttt gccaatgaag aacccgggct ggtaaaccct
     1201 gtaacaaggt ttgagattga caaggaagaa tattttagtc cgtttgacaa ctatgttaag
     1261 gttccgatca cgacttccga caactattcg ctgacatatt acgcggtgac actctttcag
     1321 ggattgctag attttcacaa aaacgacgtt aatcccgagg cactaatcga tgatgacctt
     1381 aaacgccttg attttatcag acaaaaatcc atacaccctg ataaggacac cctgtatttg
     1441 caggcgctgc aagcactatg gaaaaagttt gagacctttc cctcctcttc cgaagtaatt
     1501 tataaaatag ccaattatta ctacaccgaa ggaaacaatt atgcaacggc caataacgaa
     1561 attgaccggt ggaaactaaa aaaagcctat gaattatgcg acatcgccat aaaaaaatat
     1621 cctgatgcct ttggctcaaa aaactgcatc gcattgcaga aaaaaataaa ggagaggtcg
     1681 ctggttctca ccgcagaaaa tgtcaatgtc cccgaccagc cattccgcgg gctgcttact
     1741 taccgcaaca ttagcaaagt acatatcaga atatgcaaaa ccaattatga atggtttgat
     1801 aaactgacac atgaatatta ttacgatgac aagttaatca aagccctggt aaagcttacc
     1861 ccatcgaaac aattcagtgt ggatcttccc gatcctaaag actatcaggg acattcaaca
     1921 gagattaaat ttcccgaact tgaaaaaggg ttttatatca tccttatcgg aacggacgct
     1981 ggctttacct ataataccga agcggtggcc tatactgatc tgtgggtatc aaacatttca
     2041 tacatgaaac gaacccttac cagcggcaac acacagtttg ttgtgctgga caggcaaaac
     2101 gggcaaccca tgaaaggtgt ttcagcacag gtatttagtg aagtttataa ttactccaca
     2161 cgcagatacg aataccatag cggtgcccga tatacaagca acgaagacgg aatggttgaa
     2221 gtaatatcaa ccaacaataa ttacaacaac tcttttgtgg agtttaccta taataatgac
     2281 aagttgaaat catcagattt ttacatgtac aaagcgccca aatacgccaa tacaaaacaa
     2341 aacaggacaa tatttttcac cgacaggatg atataccggc ccgggcagac agtatacttt
     2401 aaaggtttat tgctggaaac tgatggagaa acctcagcca taaaaccgtt gacggaaacc
     2461 aatgtgattt tgtacgacgt caactaccag aaagtgaccg aaaccaagct gacgagcaat
     2521 gaatacggca ccttcagcgg cacatttatc ttacccaccg gatcgcttaa cgggcagatg
     2581 catcttgccg attcgtgggg cagcctttat ttctcagtgg aagagtacaa aagaccgaaa
     2641 tttgaggtaa aatttgaacc ggtaaaagga acttacaaac ttggtgagaa agttaccgta
     2701 aaaggttctg ctgttgccta tgcagggtcg aacatcgaca atgctcaggt aaaataccgc
     2761 gtggtgcgca ccgtcaggtt cccttactgg tggtactact ggtacgggta ttatcccagc
     2821 tcgccacaaa tggaaatcct gaacgggagc accgtaacca atgaaaatgg cgaattcagt
     2881 attgatttca cagctatacc cgattacaaa gtgagtaaaa aatctgatcc gacatttacc
     2941 tatcagatca ctgctgatgt taccgatatc aacggagaaa cacgatcttc gcaaaccatc
     3001 atgaatgcgg gatacaaagc actgcttgta aatgttgaca tgcccggtga aatagaaaaa
     3061 agcagcaaag acacctacgt tatctccaca accaatttaa gcggctctta cgaatatgca
     3121 aaaggcaaca tcgtaatcca ccagctgaaa cagcctgaaa aacttttccg caacaggtta
     3181 tggaaagagc cggatatgaa aacaatctca aaaaacgatt attacctgtg gttcccttat
     3241 gatcagtacg agaacgaatc caacacctcc aaatgggcca aaggaagcaa attactggaa
     3301 aacacttttg atacccaaaa agactcgctg ctttctcttc ccaacctttc ctcgtggcct
     3361 tcaggacaat atgtattgga gattaactcc gttgataaat ttggtgaaaa ggttcagtct
     3421 ttttcttatt tcacagtgta ctcaaatgcc gataaaacga ttcctgtgaa tgattttgca
     3481 tggttctcca cattaaaagc caagggcgaa cccggcgaag aagcagcctt tttgataggc
     3541 accaaagaac aaaatgtaag cgtgctgatg gaagtggaag tccggaacaa tattattcag
     3601 aaacaatgga taaaactcag taatgaattg agaaaaattt ccatccccat caaagaagaa
     3661 taccgtggca acttcgccgt tcatttttct tttgtgcgtc atagcagggt gttccagtac
     3721 aatgttagcg tagaagttcc atactctaac aaggaattgg acattacttt tgaaacattt
     3781 cgtaataaac tgcttcccgg acaaaacgaa gaatggaaaa tcaaaatcaa gggaaagaat
     3841 ggcgaaaagg tagccgccga aatgctggca ggaatgtatg atgcgtcact cgatgcttac
     3901 cgccctaact catggttctt aagcctttat aatcactatt acatgcaaaa atactgggat
     3961 gcgaccggtg gcttttcgtc tgttggctcg tctatttatt ctgtaaactg gaacccttat
     4021 gtgtatttca attatcgcgc ttatgattac ctgaactggt ttgggtatga caactattac
     4081 tatcgttata accgtggtta tgcttattat gccgactacg atggagcagg aattgatgag
     4141 gcagaaatta atgatatttc ggttactaca ttacaaagca aaaacggaag ggctatgaaa
     4201 aaagcggaag cagctgcacc attggcaggt gcgacaggtc atgccgaagg ggatgttgcc
     4261 atggcaaatt tcgaaacttc tgcgactgaa caatccaagg ataaggcaag cggcggcgaa
     4321 aaaaaagaca gccgccagga tggtctgaca gacataaaaa cccgaaccaa cttcaacgaa
     4381 acagcgttct tctaccctca cctgcttaca aacgaaaacg gtgagattgt cataacattt
     4441 accgtgcccg aagcgcttac caagtggaag tttatgggac tggcacacac caaagacctt
     4501 aagttttcgc agattcaaaa agaggtactc acccagaaag acctgatggt gatgcccaat
     4561 cctcctcgct ttttccgtga aaacgacaaa atagttttct ccgcgaaaat caccaacctt
     4621 gccaatgatt ccattactgc cgatgcctct ctcatgctct tcgatgcaag cacacagaaa
     4681 cctattgacc tgctgatgaa aaacaccgat caggtgaaat gtgtaggaat acccaaggga
     4741 caaagcaaag ccgtggaatg gactttatca gtaccggaaa acattgctgc cattacctat
     4801 aaggttgttg ccaaagcggg aaatttcagc gacggcgaag aaatgaccat tcccgtgctg
     4861 acaaaccgaa tgctggttac cgaaacgttg ccactgccta tccgtggcaa tcagtcaaag
     4921 aacttttcgc tcgaaaagct catcaatgcc tataaatcca acacgctgaa aaattacaaa
     4981 ctcacgctcg aatttacatc caacccttcg tggtatgcgg tacaggccct gccctatctg
     5041 atagaatacc cgtatgaatg taccgaacaa attttcagcc gtttttatgc caacagcatc
     5101 gctacctaca tagccaactc caaccctaaa atcagagaag ttttcgacag ctggaaaaac
     5161 atcactcccg atgcgctcct atcaaacctt gaaaaaaatc aggacctgaa ataccttatg
     5221 ttgcaagaaa ccccctgggt gatggaagcc aaaaacgaat cgcagtcaaa acgtaatatt
     5281 ggcgtgctac tggaagccaa acgtatgcaa ggcgaaatag aaaaagcact aaaaaaactt
     5341 gaaaaactgc aggttccaaa cggcggttgg acatggtttc ccggcatgcc tgatgacaga
     5401 tatatcaccc agcatatcat cacaggtttc ggccatctcg acaaacttgg cgtccgtgat
     5461 gtccgggatc aatataaagt atggaacatg gttaaacgag gctcgcgtta ccttgatgac
     5521 cgtgtgaggg aggactatga atggctgtta agatatgatc cggatcatat gtttgaaaat
     5581 caccttggag ggatacaaat tcagtatctt tatgcacgca gctatttcat caaagatatt
     5641 cccatagagt ctaagaataa aaaggccttc gactattatt tcggacaagc aaaaaaatac
     5701 tggttgccca acaaccggta cctgcaaggc atgatagccc tggcaatgaa tcgcctgaac
     5761 gataaagcaa cggctacaga tatcgtaaaa tcattgaaag aaaatgcaat tttcaatgat
     5821 gaaatgggta tgtattggaa agatatggat tacggatact attggtacca ggcacccatt
     5881 gaaacacaag ccctgctgat tgaatgtttt gatgaagtgg ccaacgacaa gcaatccgtg
     5941 gaagatatga aggtatggct gctcaagcag aaacagacac aaaactggaa aaccaccaaa
     6001 gccacagcgg aagcttgtta tgctctgttg ctgcggggca ccgactggct cgccagagat
     6061 cctcaggtaa gtatttcgct gggtggaatg cttgttgatc ctaaaaacct tcctgatgct
     6121 aaacaacagg cgggtacggg atatttcaga acatcctggt ttgatgaagc tattaagccg
     6181 gagatgggaa aagtgaaagt cagcaaaacc gacgatggtg tggcttgggg agctctgtac
     6241 tggcagcatt ttgaacagtt ggataaaata acagttgcag aaacgccgct caaactggga
     6301 aagaaacttt ttgttgaacg caactcagac catggtccgg tgattactcc ggtaacagag
     6361 gatacccaat tgaaacttgg agacaaaata aaagtacgta ttgaactccg cgttgaccgc
     6421 gacatggaat acatccacat gaaagatatg cgggcatcgg gatttgaacc gataaatgtg
     6481 ttgtcgcaat acaaatacca gggcgggctg ggatattacg aaagcaccgg cgatgctgcc
     6541 accaacttct ttatttcgtt tttgaaaaaa gggacatacg tttttgaata tccgctgcgt
     6601 gtggcgcaga aaggcgattt ttccaacggg gttactacca ttcagtgtat gtatgctccg
     6661 gaattttcgg cccattccga aggaatacgg gtgaaagttt cgggataagg tatatagaac
     6721 taacaggttt acaattttta ataagaacta acaggtttgc aataatagag gttttcgtaa
     6781 cttatattgt ttgtaaacct gttagttctg atttgtaaac ctgttggttt ttaatattac
     6841 aaatcttcga taatcccttt taacagccat tccttctgac aatgccacgc ttcaaaattg
     6901 gcgatattac cgaaaaagtt gataacgaaa tctttatcaa taaaactttt tctttcagaa
     6961 aaaaaatcgg catatgaaga gtgaggataa ttcctgaaat cttttacgaa cccatgtttt
     7021 tctgggttga ggtgaatgta ggccaccaca tttttaaaaa aatcatcttc tgtaatccgg
     7081 tttcggtgaa aatgctcctg aaacaggctg ccggtgcggt tatacgcttt atttatgctt
     7141 tttgtataag aattaaaaaa atgtgaaaaa gcaagatgtg gtttttttcg gtatttttct
     7201 tcagcaatat ttgtggattc ttttatcttc aacaacaaat gaaaatgatt tttcatcaga
     7261 caatagcaat aaatatcagc gatatttgca aggtatttct ccatcaagga gataaaatac
     7321 ttataatttt tttcttcgat aaaaaggttt tccttattgt ttccccgatt aaagatatgg
     7381 taaaccgccc cagattcgat agcatcaatt ttcacataca aagtttttaa ctaaaatagt
     7441 aattttttaa aggtgtcgtt aaacacctaa caggttttct aattaatatt ttcgaaatat
     7501 tttattattg aaaacctgtt aggtgtcggt ggttgatggt agaaaaaaat tactatttta
     7561 gtagttcaaa aaaatattga aagatgtttg catttggaaa taaacacccg tatcatgagg
     7621 tcattatatc aacccagatt aaaagcaata accctgttgc tcttgccatt catgatcagc
     7681 gccaatacgt tgtcgatggt tcaaaaagcg ccacctactg cggtaatttg cgctccggag
     7741 taggaatacc atacgacatc actacaaaat cagggaaacc ctttgcggat gatatgcttg
     7801 atgttgttgg caaggccttg aaaaaaaacg gtatcaaatt aagcaaagta gtaacacgat
     7861 cgggactaac caaagaggca gtcatagccg aactggtaaa aacacaggcg ccacggtcgc
     7921 tgtacatcag gatactggaa tggaaaaccg acacttatat gaaagctgac ctttattgcc
     7981 ttgtcttgct gcaagtgctg gacaacaacg gtaatattgt tgctgaaaat agcgttaaaa
     8041 caagccgcga agcgttcgga ggcagttttt ggagtcctca gaaagctgct aaaaaaggcg
     8101 cgttgaaagc gcttaaaatg aaaatggaag cactgattaa tgaaccgaat attattaaag
     8161 catttgaata agcgaatttg aaaaatttta ttaaatcatt acagcggaaa ataatactgt
     8221 tggcgatcat tattattgat tgctttgcct gcaaagctca ggaatcaatt attccgatcc
     8281 ggtgcgacag catgtattat gtctccatac ttgattcgct gaaaagttct tacccaaact
     8341 gctacaacat ccctccggaa tttgaactgg ctttttatac tgcgatttcc cactatccgg
     8401 aacttaaggc aaccaatatt gaaatgaagt tgaaaaaatt caactttagc atggctgccc
     8461 ggcctgcaaa aaaactgctg gcaaaccgca agaaacgtat ttaccgtgta tatgcaaaca
     8521 taaaaaaaga tttcaccggc ctgctgccct cacgcctcaa ctataatcaa agaacaggaa
     8581 tcatcggaca tgaactgggc cacattcttg attattcaaa taaaaatatg tgcaaaatga
     8641 tagggaccgg cattggttat atgtccagag cataccggcg taaactggag gcaaaaaccg
     8701 acatcgaagc tatcagacac ggtctcggat ggcaactata tgatttcaat aattacctgt
     8761 ataactattc ggatgccagc gaaaaataca aaaagaaaaa aaagagaatc taccttgatg
     8821 aaaaagaaat caaagaactt attattaata attcaaaata attaacggat gaaatgcttg
     8881 aacatactga attatttaat tatactgtta ataagtatta atacttattc gaacacctat
     8941 accgttgtta atacattgag taacggtgca ggct
//
LOCUS       DAOSFP010000046         8917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6676_length_8917_cov_3.745430, whole genome shotgun sequence.
ACCESSION   DAOSFP010000046 DAOSFP010000000
VERSION     DAOSFP010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6676_length_8917_cov_3.745430"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2718
                     /gene="sprA"
                     /locus_tag="PLI16_02365"
     CDS             <1..2718
                     /gene="sprA"
                     /locus_tag="PLI16_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOH83432.1"
                     /translation="LRLTDFDQSGGYAATGRIAANLADFGNVSLAGTISTPGFGSIDK
                     KLNQLQKETIISYDIATNLELGKFFPEKWGVKIPMHFDYSEMRTVPEYNPLNPDVIYR
                     EDLKTYSKTARDSVRKITQDVTTRKSLNFMNVRKTKVGASTKNHIYDLENFDFTYAFT
                     ELFHRNVDIEYDRRVTHMGGLGYNYSANPKIVKPLDKWKLISKSKWLRIIKDFNFYYM
                     PKLLSFRTDLNKQFQENLMRNKTNAIIILEPAYVKRFGWTRDYALKWDLTQNLKLDYQ
                     AHAIALIDEPPGKIEKGQPNFKQNRDSIWENVLNFGRMTNYNQNIDINYTLPINKIPA
                     FNWMNISARYGGAYSWTALPLSTDSLDNIIENPLGNVIENSNSEQLNLTANLTNLYNK
                     VKYLKKINDKRRGGGSSKPQIKTNMPGENPDSLKPKINIGKEILDNTLSLLMLVKTVN
                     ISYQESNGRLLPGFMHKPVALGMDWKHKAPTAGFVFGSPGDMFASTGDILGKAFQNNW
                     ITADTSLNTPFSVKQSKVLNYQASLEPLNGMRIDVTGNTNYAKQNQAFYRYDMDGENN
                     HSEPFSLVETGNFSMTIIGMKTAFIADNKDNSNDVFQKFKDYRIIIATRLAAQNPNST
                     QQIVDSTGFPDGYGPTSQDVLIPAFLAAYLGKSPDNISLTPFSEQILKAIPLPNWRLT
                     YNGLTKIGFIKKYFRTVTLSHTYRCTYNVSSFATNVRYKEGEDGFSFIRDQIGNFIAE
                     KEIGQISITEQFSPLVGLDLTMINSLLVKFEWKKSRNLSLSFANNQLTEVASNEYVVG
                     AGYRFKDVSFNLNLGGKRRHIKSDLNLKADFSVRQNKTTLRKLVENMDQVSAGGQIIS
                     IGVSADYQISEKFNVRLFYDHIINNPYVSSQYPNSNINGGLSLRFTLAQ"
     gene            2773..3153
                     /gene="gcvH"
                     /locus_tag="PLI16_02370"
     CDS             2773..3153
                     /gene="gcvH"
                     /locus_tag="PLI16_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOH83433.1"
                     /translation="MKIEENLLYTESHEWVRIEGENAYIGISDFAQHELGDIVFVEVD
                     TIGETINKGEAFGTIEAVKTVSDMYLPLSGEILELNPVIETNPETINKDPYGEGWIVK
                     IKLTNPEEAGNLLKPADYAKLVKA"
     gene            3315..4082
                     /locus_tag="PLI16_02375"
     CDS             3315..4082
                     /locus_tag="PLI16_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOH83434.1"
                     /translation="MISYLNDIDEIIFRNRNKEYGAYYLRKNYNRYLVVATILAVTFF
                     VLCWLVPAMTVKPPEKKLNEGALYTEYLGPPPKEIKKPDEDLLPQLQKVQKMARFVIP
                     QVVINLEDENSEISLESRPGDTASDGNSSSGSPQGALEGGGGDPDAVYTYVEEAPEFP
                     GGESARMLFLRNNIKYPQLALQNKIQGKVYISFIIERDGSVSNVKVLQGIGAGCDEEA
                     LRVTRLMPKWKPGKTQGHEVRVVINMPVNFVLQMSKT"
     gene            complement(4290..6479)
                     /locus_tag="PLI16_02380"
     CDS             complement(4290..6479)
                     /locus_tag="PLI16_02380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH83435.1"
                     /translation="MAVIGKLRKHSALIVILVGIAIAGFVLQDLFTGGKGGRRSNEIF
                     AKIGSEKLSKIDFDKKVDEQVEYYKQQAEKENLTSEENFQLMVQTWNQMEKELIMQHE
                     YEELGLAIEHDKSNKPSISPEELYELMMGKNLHPYIIRSFTDPNTGKVNTQQIQNIIT
                     NFDQLKDDEKKQWKQLEQGIKDDRLATKYNNLVEKAYYMPKVFLQRQINEASKTANLR
                     CYGIKYQTISDSAVTVTDDDMKKYYEEHKHEFEQENARDIDYVVFDVLPSKEDLAQIQ
                     EKVDTLFKDFQALDAENIENFIKANSDAPYDSNFYKKGSLPVFLDSIMFNAPLGTIVP
                     PYVDNNSYFMARLMQIQNRPDSVKASQLLVAYKDAPNPLQNITRTKEQAKRIIDSLLV
                     VANADKNNFPTLASINSDFPTAKQDGGDLGWIIDGNVETKFFFDSAYNSPVGNIKVIQ
                     STMGYHLLYVADKKASMKKVKVAILQHDIKASSETFNKYLADASAFAGESRNTEQFNK
                     NIADKGLNKRQAQFVKEMDYTLPGLTSAREIIRWAFYKETEKGMVSEQVYDCEGKYVV
                     AMLVEKREKGIAPLDQVKTYIEPLVKREKKADQIIQKINATAGASKDINQIAAKMSTP
                     VDTVNALTFSAYNFPKFGPEPELIGTIFTLKKNTLSAPIKGKMAVYQVWIDDISQAAG
                     QINPDMMQMQLASYFRQRVRGDQYGQSELFNAIKKQTEITDNRIFYY"
     gene            complement(6537..7823)
                     /locus_tag="PLI16_02385"
     CDS             complement(6537..7823)
                     /locus_tag="PLI16_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOH83436.1"
                     /translation="MDNNIILVFITILFSAFFSGIEIAFISANKLQIELDKKNRLFSG
                     RMLSGFLKNPSDFISTILLGSTITLVIYGIAMSHLLEEPIRRLLPENLHSGFTTTILQ
                     TLVSTLIILFLGEFAPKAFFRSNPNGFLKFFSFPLFIIYYLLFPLQFIFVELSEFILE
                     RIFRIKLKTEKYKFDHTDLDYYVKDLSAAEHEDESHMPEIQMFQNAIDFTSTRVRECM
                     VPRNEIIAIEENESIEELIKKFVNTGHSKILVYQKHIDNIIGYVHSYDVFRNPRSIKS
                     INKPIIIVPETLLAKEVLPMFIQQHKSVAVVVDEFGGTSGMVTLEDLLEEIIGEINDE
                     FDESAPIEKQLSENELLLSGRLEIDYLNEKYLLHLPVNDDYETLAGYIIHHLERIPAE
                     KETFHIPPYDITILKASNKRIEQICLTKRSDENILT"
     gene            complement(7827..8414)
                     /gene="lptC"
                     /locus_tag="PLI16_02390"
     CDS             complement(7827..8414)
                     /gene="lptC"
                     /locus_tag="PLI16_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOH83437.1"
                     /translation="MNTVSVLTKKLVVFSLALLMFVASCKNKIEDINALTLSDTLPVE
                     TGKNIEIIYSDSAKIQTLIKSPFYKKYAGKNPYIEMPQGIDAIFYDSAMRIKTRLTAK
                     YAIKYDKKHVMEVKNNVVVINEKKERLNTEHLIWDEQLRRIYTDEFVKITTPDKVFYG
                     DGLDADDSFMKWVIKKPRGTFYVNTDEGEDSENNN"
     gene            complement(8418..8720)
                     /locus_tag="PLI16_02395"
     CDS             complement(8418..8720)
                     /locus_tag="PLI16_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83438.1"
                     /translation="MGGEYSPRANTITSYWKLINYRAGLRYGQSYYEIRDKKINDLSF
                     SIGAGFPLPKTRTTLNLAVEAGTRGTVRNDLLQENYIRLVLGISFREFWFFRPKLN"
BASE COUNT         2514 a         1830 c         1927 g         2646 t
ORIGIN      
        1 cttcgtctga ccgattttga ccaaagcgga ggctatgcag ccaccggcag gatagctgcc
       61 aacctggccg attttggcaa tgtaagtctc gctggaacca tcagtactcc tggctttgga
      121 agcatcgaca aaaaactgaa ccaactccag aaagaaacaa ttatctctta cgatattgcc
      181 accaaccttg aattaggaaa gtttttcccg gaaaaatggg gtgtaaaaat tcctatgcac
      241 ttcgattatt ccgaaatgag aaccgtgccg gaatacaacc cgttaaatcc cgatgttatt
      301 tatcgcgaag acctgaaaac ctattcaaaa acggcccgcg actctgttag aaaaattacc
      361 caggatgtaa ccacccgtaa gagcctgaac tttatgaatg tgcgtaaaac aaaggtgggc
      421 gcatcaacca agaatcatat ctacgacctc gaaaattttg atttcaccta tgcctttacc
      481 gaactgttcc atcgcaatgt agacatcgaa tatgacaggc gggttaccca tatgggcgga
      541 ctgggatata attattcggc aaatccgaaa attgtcaaac cgctcgataa atggaaactg
      601 atatctaaat caaagtggct gcgtatcatc aaggatttca atttctatta catgcctaaa
      661 ttgctgtcgt tcagaacgga cctgaataaa caatttcagg aaaacctgat gagaaacaaa
      721 accaatgcga taataattct ggagcctgcc tatgtcaaac gcttcggatg gacacgcgat
      781 tatgcgctga aatgggattt gacacaaaat ctaaaactcg attaccaggc acatgccatc
      841 gccctgatag atgaacctcc cggaaaaata gaaaaaggac agcctaattt taaacaaaac
      901 agggattcta tttgggaaaa cgttctgaat ttcggacgta tgaccaatta taatcagaat
      961 atcgatatca attataccct gccgataaac aaaattcccg cctttaactg gatgaacatt
     1021 tcggccaggt atggcggtgc ttactcttgg acggccctgc cgttatctac cgactcgctg
     1081 gacaacatta tcgaaaaccc gctcggcaat gtaatcgaaa actccaattc cgagcagtta
     1141 aaccttacgg ccaaccttac caacctgtat aacaaggtaa aatacctgaa aaaaatcaac
     1201 gataaacgcc gcggaggtgg ttcctccaaa ccgcagataa aaaccaatat gcccggtgaa
     1261 aaccccgatt cattgaagcc caaaattaat atcggtaagg aaattcttga taatacctta
     1321 agtttgctta tgctggtcaa gaccgttaat atttcgtatc aggaaagtaa tggccgcctg
     1381 cttccggggt ttatgcataa acccgtggct ctgggtatgg actggaaaca caaggcaccg
     1441 actgccggat ttgtctttgg cagcccgggc gatatgtttg cttctacagg tgatatcctt
     1501 ggaaaagcct tccagaacaa ctggataaca gcagatactt cgctgaatac tcctttttcc
     1561 gttaagcagt caaaagttct gaactaccag gcctcgctcg aacccctcaa cggaatgcgt
     1621 atcgacgtta ccggaaatac caactatgcc aaacaaaacc aggcgtttta ccgctacgat
     1681 atggacggtg aaaacaacca cagcgaaccg ttttcgctgg tggaaaccgg aaatttcagc
     1741 atgaccatca ttggcatgaa aaccgctttt attgctgaca acaaagataa ctccaatgat
     1801 gtgttccaga aattcaaaga ttaccgtatc atcattgcaa cccgccttgc ggcccaaaat
     1861 cctaactcta cacagcagat tgttgactca acaggattcc ccgacggcta tggtcccacc
     1921 tcgcaggatg tcctgatacc ggcatttttg gccgcatatc tgggcaaaag ccccgataat
     1981 atctccctga cacctttctc ggaacagata ctgaaagcca taccattgcc aaactggaga
     2041 ctgacataca acgggttgac aaaaatcggc ttcattaaaa aatatttcag aacagtaacc
     2101 cttagccata cctatcgctg cacatataac gtaagttctt ttgccaccaa tgtcagatac
     2161 aaagagggtg aagacggttt ttcgtttatc cgtgatcaga ttggtaattt catcgctgaa
     2221 aaggaaatag gacaaatatc aatcacagag cagtttagcc cattggttgg gttagacctt
     2281 accatgatta acagcctgct ggtaaaattt gaatggaaaa agtccagaaa cctctcgctg
     2341 agttttgcca acaaccagtt gaccgaagtc gccagcaacg aatatgtcgt tggtgccggg
     2401 tatcgtttta aagatgtttc attcaacctg aaccttggcg gaaaacgccg gcatataaaa
     2461 agcgacctta acctgaaagc cgatttttcg gttcggcaga ataaaaccac ccttcgcaaa
     2521 ctggttgaaa atatggacca ggtgtcggct ggcggccaga taatttccat tggggtttcg
     2581 gctgattacc agatcagtga aaagtttaat gtgcgcctct tctacgatca tatcatcaac
     2641 aatccctatg tgtcgtcgca ataccccaat tccaacatca acggaggctt gagcctgaga
     2701 tttactttag cacaataata gttgcattaa taaaaagttg tatatttgac aaaaaaataa
     2761 atcacaatca tcatgaaaat agaagaaaac ttattgtaca ccgaatctca cgaatgggtg
     2821 agaattgaag gcgaaaatgc gtatatcggt atttccgatt ttgcacagca tgaattaggc
     2881 gatattgttt ttgttgaagt ggataccatc ggcgaaacta ttaacaaagg agaagctttt
     2941 ggcaccatag aagccgttaa aaccgtttct gatatgtatc tccctctcag tggagaaatc
     3001 ctcgaactga accccgtcat tgaaacaaac ccggaaacta ttaataaaga tccttacggc
     3061 gaaggctgga tagttaaaat aaaactgacc aatccggaag aagccggcaa tttattaaaa
     3121 ccggccgatt atgctaagtt agtaaaggcc tgattttttt aaaacatgca attttaagct
     3181 ctcacctgtt acagtgaggg ctttttttta tttatatgtg ccctctgcaa aataaaataa
     3241 taaaaccccg ttatatttat tgacttttat aattttggca ttaagattga gcaaaatttc
     3301 aataaatcaa ggcagtgatt agttacctga acgatattga cgaaataatt tttcgcaacc
     3361 ggaataagga atatggcgct tattatttgc gaaaaaacta taacaggtat ttggttgttg
     3421 ccactatcct tgccgtgacc ttttttgtgt tgtgctggtt ggtgccggcc atgacggtga
     3481 agccgcctga gaaaaagctc aacgaagggg ccttgtatac agaatatttg gggcctccgc
     3541 ccaaagaaat aaagaaacca gacgaagact tgctcccaca gttgcagaaa gtgcaaaaga
     3601 tggccaggtt tgttattcca caggtggtca taaaccttga ggacgaaaac agcgaaatca
     3661 gcctggaatc gcgtccgggt gatacggctt cggacggcaa cagttcttcg ggaagcccgc
     3721 agggagcgct cgaaggcggt ggcggtgatc ctgatgctgt atatacttat gtggaagaag
     3781 ctcctgagtt tcccggagga gaaagtgccc gtatgctgtt tttgcgaaac aatatcaaat
     3841 atccgcagtt agctctgcaa aacaaaattc aggggaaggt gtacatctct tttatcattg
     3901 aaagagacgg cagtgtcagc aatgtcaagg tattgcaggg tattggagcc ggctgcgatg
     3961 aagaagccct gcgggtaacc cggctgatgc ctaaatggaa gcctggcaag acacagggcc
     4021 acgaggtaag ggtggttatc aatatgccgg taaattttgt attgcagatg agcaagacgt
     4081 aatatccgga tacaacaaaa ttatgctaaa aaaacagtga aaagctatca ctaaacgaaa
     4141 gtgttgacat tggttgaaca aagccacccc ccgcaaattt ggcttgcaag gcggtcaaac
     4201 tttcataaat aaaaacggtt ctgactaatc atcagaaccg ttttttatgt atgggaagcg
     4261 cctgaagggc ggcacttgct ccgattattt tagtaataga atatcctgtt gtcggtaatt
     4321 tcagtttgtt ttttgatggc gttgaacaat tcgctttgtc cgtattgatc gcctcttacg
     4381 cgctgacgga aatagctggc cagttgcatc tgcatcatgt cgggattgat ttgtcctgct
     4441 gcttgtgaaa tatcgtcaat ccatacctga tacacggcca tttttccttt gatgggggca
     4501 gagagggtgt ttttctttaa ggtaaaaatt gtacctatga gttcgggttc ggggccaaat
     4561 ttcgggaagt tataggctga aaaagtgagt gcgttgacgg tatctacagg agttgacatt
     4621 ttggcggcaa tctgattgat atcttttgat gcacctgcgg tcgcattgat tttttgaatt
     4681 atctggtcgg cttttttctc gcgttttacc aatggttcga tataggtttt tacctgatcc
     4741 agcggagcaa tacctttttc gcgtttttca accagcatgg ccactacgta tttcccttcg
     4801 caatcataca cctgttccga aaccattcct ttttcagttt ctttataaaa agcccaccgg
     4861 atgatttccc gggcagaggt aagacccgga agagtatagt ccatttcttt gacgaattgt
     4921 gcctgtcttt tgtttaagcc tttatcggcg atatttttat taaattgttc ggtgttgcgg
     4981 ctttcaccgg caaaagcgct tgcatctgcc agatatttgt tgaaagtttc gctgctggct
     5041 ttgatgtcgt gctggagtat ggctactttc acttttttca tcgaagcttt tttatcggct
     5101 acgtaaagaa ggtggtaccc catagttgac tggatgactt ttatgttgcc taccggcgag
     5161 ttataggctg aatcgaagaa aaactttgtt tctacattgc catcaataat ccatcccaga
     5221 tcaccgccat cttgtttggc tgtggggaaa tcggaattga tacttgccag tgtggggaaa
     5281 ttgtttttgt cggcattggc aaccaccagt aagctgtcaa ttattctttt ggcttgttct
     5341 ttcgtacggg taatgttttg caaaggattg ggagcatctt tatatgccac gagcagttgg
     5401 cttgctttca ccgaatcagg acggttttgt atctgcatga gccgtgccat gaaataggag
     5461 ttgttatcaa cgtatggcgg tacgatagtc cccagtggtg cattgaacat gatggaatcg
     5521 aggaagacgg gcagggaacc ttttttataa aaattgctgt cgtatggggc atcgctgttt
     5581 gctttgatga agttttcaat gttttcagca tccagagcct gaaaatcttt aaacaaggtg
     5641 tccacttttt cctgtatctg tgctaaatct tcttttgatg ggagtacgtc gaaaacgaca
     5701 tagtcgatat cccgggcatt ttcctgttca aattcgtgtt tgtgttcttc ataatatttt
     5761 ttcatgtcgt cgtcagtaac ggtgacggca ctgtccgaaa tcgtctggta cttgattccg
     5821 tagcaacgga ggttggctgt tttgctggct tcattaatct gcctttgcag gaatactttg
     5881 ggcatataat aagctttttc aaccaggttg ttgtatttgg tggccagcct gtcgtctttg
     5941 ataccttgtt ccaactgttt ccactgcttt ttttcatcat ctttcagctg gtcaaaattg
     6001 gtgatgatat tttgtatttg ctgtgtattc accttgccgg tatttgggtc ggtgaaacta
     6061 cggatgatgt atggatgcag gtttttgccc atcataagtt cataaagttc ttcgggcgaa
     6121 atggatggtt tgtttgattt atcatgttcg atggccagac cgagttcttc atattcatgc
     6181 tgcataatca gttctttttc catctggttc caggtttgca ccatcaactg aaaattctct
     6241 tccgaagtga gattttcttt ttctgcctgt tgtttgtaat attcaacctg ttcgtccacc
     6301 tttttgtcaa aatctatttt gctgagtttt tcactgccta tttttgcgaa aatttcattt
     6361 gaacgacggc cccctttgcc gccggtgaat aaatcctgca gcacaaaacc ggcaatagca
     6421 atacctacca ggataacaat cagcgctgaa tgtttacgta attttcctat aactgccata
     6481 aaaatttatt tttttgagtt tgcaaatgta caacaataag tgaaatatta acagaattat
     6541 gtcaaaatat tctcatcact tcttttggta aggcaaatct gttcaatacg cttattgctg
     6601 gccttgagaa ttgtgatgtc atagggaggg atgtgaaatg tttctttttc ggcggggatg
     6661 cgttcaagat gatgaatgat gtagccggcc aatgtttcat aatcatcgtt aacgggcagg
     6721 tgaagcaggt atttttcatt caggtagtcg atttcgaggc ggcccgataa aaggagttcg
     6781 ttttcgctga gttgtttttc aatgggagca gattcatcaa attcgtcgtt tatttcgcca
     6841 atgatttctt ccaaaagatc ttccagggta accatgcctg aagtgccgcc aaactcgtca
     6901 accaccacgg ctacactttt gtgctgctgg ataaacatcg gtagcacttc tttggccagt
     6961 aatgtttccg gcacaatgat aatgggtttg ttgatggatt ttatgctgcg tggattgcgg
     7021 aaaacatcat acgaatgaac atatccgata atgttatcaa tatgtttttg gtacaccagg
     7081 atttttgagt gtccggtatt tacaaatttt tttatcagtt cttcgatgct ttcgttttct
     7141 tcgatagcta ttatttcgtt gcgcggcacc atgcattccc tgacacgggt gctggtgaaa
     7201 tcgatggcat tctggaacat ttgtatttcc ggcatatgcg attcatcttc atgttcagca
     7261 gccgaaaggt ctttaacgta ataatccaga tcggtatggt cgaatttgta tttttctgtt
     7321 ttcaacttta tcctgaaaat acgttcaaga atgaactctg ataattctac aaaaatgaac
     7381 tgcaggggga aaagcaggta atagatgata aacaatggaa aagaaaaaaa tttcaggaat
     7441 ccgttgggat tgcttctgaa aaaggccttg ggggcaaatt ctccaagaaa cagtattatc
     7501 agggtggaca ccagggtttg aagaatggtg gtggtgaagc ctgagtgcaa gttctcgggc
     7561 aacaaacggc ggataggttc ctccagaagg tgcgacatag cgataccgta aatcaccagg
     7621 gtaatggtgc tccccagcag gatcgtagaa ataaagtctg aaggattttt tagaaatccg
     7681 gaaagcatcc ttcctgaaaa caatctgttt tttttgtcca gttcaatttg aagcttattg
     7741 gcactgataa atgctatttc tatacctgaa aaaaaagcag aaaacaaaat ggtaataaat
     7801 actaaaatta tgttgttgtc cattgcttag ttgttgtttt cagaatcctc cccctcatca
     7861 gtgtttacgt aaaaagtgcc ccggggtttt tttattaccc atttcatgaa ggaatcgtcg
     7921 gcatcgaggc cgtcgccata aaatacttta tcgggtgtgg tgattttgac aaattcgtcg
     7981 gtataaattc ttctgagctg ttcatcccat atcaggtgtt cggtgttgag cctttctttt
     8041 ttttcgttga taacgaccac gttgtttttt acctccatca catgtttttt atcgtatttt
     8101 atggcatatt ttgccgtaag gcgggttttt atacgcatag cagaatcata aaaaatagca
     8161 tcgataccct gcggcatttc gatatatgga tttttgccgg cgtatttttt atagaaaggc
     8221 gattttatga gggtttgaat ttttgccgag tcgctgtaga ttatttcaat gttttttcct
     8281 gtttccacgg gcagggtatc cgaaagtgtc aacgcattga tgtcttcgat tttatttttg
     8341 caggaggcaa caaacatcaa taaagccagc gaaaaaacaa caagtttttt tgttaaaacg
     8401 gatacggtat tcatttttta attcagtttg ggcctgaaaa accagaattc cctgaaagaa
     8461 atgcccaaga caaggcggat atagttttcc tgtaagaggt cgttgcggac agtgcctctg
     8521 gtgccagctt ccactgcaag gttcagcgtt gtccgcgttt taggcagtgg gaatccggcg
     8581 ccaatactga aactcagatc gttgattttt ttatcacgga tctcgtaata actttgccca
     8641 taacgcaatc ctgcgcggta attgataagt ttccaatagc ttgtgatggt gtttgcccgc
     8701 ggggaatatt ctcctcccac cgaaataata taactgttgc ctaatgacga ggcggattct
     8761 ccgaaactgg taaaattttt ccagtttttt tgatttaacc caaattaagc aataaaaatg
     8821 cttaatcggt taaaaagttg tatttcaatg atttaaaaaa tcagcttgcc ccgtttataa
     8881 cgagaggatt aacccaacta aatcttatag tttggct
//
LOCUS       DAOSFP010000047         8895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6693_length_8895_cov_3.749548, whole genome shotgun sequence.
ACCESSION   DAOSFP010000047 DAOSFP010000000
VERSION     DAOSFP010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6693_length_8895_cov_3.749548"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2228
                     /locus_tag="PLI16_02400"
     CDS             <1..2228
                     /locus_tag="PLI16_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOH83439.1"
                     /translation="DGIDNDNDGYTDNYRGWGMGENDNNPMGTISHGTFTAGLASATT
                     DNGAGIAGTGFQCRFLPIKVCNANNLGTMTYESIVYAAEHGCSIINCSWGSTFYTGQY
                     GQDVIDYATINRGALVVAAAGNDNNTAVFFPASYNYVLSVAASDINDHKANFSNYGHF
                     IDIIAPGDNVWSTGMGGIYGSSGGTSFSAPIVSGCAALLRCKYPSLSVLQIAEKLKVT
                     ADVIDTLPANLAYRDMLGAGRLNIFRALNDSVLPSVQMFNINFENSHHQALADPGDTV
                     YITSNFINYLAPSTANLTISLTCASSHVTLLDSVFVAGNLPTSGTINNSSDPFSLLIK
                     PGITPNENLVCKFTITDNNYRAVQYYYIYANKDYIDIDTNKIATTLTSDGALGYVDGN
                     SLQGLGFRYNNGETMLAAGGFIVGRSGTQVCDVLYGDPGLYDHDFTVKTSMARVFPPE
                     QADFEGKGIFNDSLAGTSKINVEVTQRVHAWKEPPNDKFILLEYTLKNKNAYALSGLY
                     AGLYADWDIDGSSTNRADYDAETKTGYSYSISGSQYAGISLISSGTATHYAFDNTGEN
                     GSINISDGFTSSEKYVALKTNRNSAGISGYGNDVSQMLSSGPHTLAANDSTVIVFALI
                     AGDHLGDIQSSATAAHQAYFHTGIVSNEPSTADVVLLQNQPNPFKENTSIGVLVMKKT
                     FVELSYCNVSGSTSGIIFSGTLDKGFHTFTTGNNISPGIYVYTLTGDGFCLKKKMCVT
                     E"
     gene            2311..2445
                     /locus_tag="PLI16_02405"
     CDS             2311..2445
                     /locus_tag="PLI16_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83440.1"
                     /translation="MILKIIKIFLLLLVIAVLSVSCETKKNCGGMKYHNDDVKRGLAH
                     "
     gene            complement(2493..3962)
                     /locus_tag="PLI16_02410"
     CDS             complement(2493..3962)
                     /locus_tag="PLI16_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOH83441.1"
                     /translation="MGNVLSNLKPTALWKNFEDICSIPHPSTHEEKLREHVLAWAKTH
                     QLECSVDDTGNVIVRKPATPGMENRKGIILQAHLDMVPQANSDIQHDFTTDPIKPRID
                     GDWVTATGTTLGADNGIGMAAAMAVLEATDLQHGPLEMLLTVDEETGMTGAFGLKEGL
                     LKGDILLNLDTEEEGDLCVGCAGGTNANMKFYYDEKPVAGNVEALKISVKGLKGGHSG
                     VDIHLERANSNKLLNRILWYAYNNLGMRLSSIDGGSLRNAIPRESFAVVTLPKENVDK
                     FKQFIKEFTEIVINEYKNVEDNITILAESVELPKTIIDNVTTNNLLDAIYGTPNGVIR
                     MSQDMKGLVETSTNLAIVKSKDGVIDVMALLRSSVDTAKDDLENMFASIYSLACAESI
                     FDGQYPGWKPNMDSPILKEMLLAYEKQFGTKPHVSAIHAGLECGLLGGVYKNWDMISF
                     GPTINFPHSPAEKVNIPSVEKFWAFLTETLKNAPVKENA"
     gene            4114..4650
                     /locus_tag="PLI16_02415"
     CDS             4114..4650
                     /locus_tag="PLI16_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH83442.1"
                     /translation="MSLDRNIIEGCIAGNRKAQKFLFEKYKAAMMGVCLRYCKSKDEA
                     EDVLMEAFMTVLSQIQSYRGDGSIDQWIRRIVVNTSINNYRKNLKHYFHADIENIAET
                     EIEEDNTFDITDNHSVEEIMNAMQQMPQGYKIVLNLYVVEGYKHKEIAEMLNITVGTS
                     KSQLSKARKIIQDKLTNK"
     gene            4665..5630
                     /locus_tag="PLI16_02420"
     CDS             4665..5630
                     /locus_tag="PLI16_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH83443.1"
                     /translation="MNEFLNNGPKNFDDYLRTALDNYQPDVSSGVWQSLKLQLFKKDA
                     IDFLTFKKLSRSFNSSANFGAAQIKVLATYAAAACLAVGVVYGASRIYKSIVTVPVEK
                     NSTTEPQTNISTEAGIPGNTADTQVAVEEHSNSSIPGNNRFNRTVVSSGAENEAGQAV
                     QAATDNGPDNSMPATSQKAENAETHTSLQNYIEKVTSQDKPSGKETVVQNLPETIDIP
                     ETPVEEMVENETDPYAYNLEIPNVITPNGDGYNDLFKIRNLDKYTNNTLTIANRNGQV
                     VFETNNYQNNWDAQNLDAGTYYYILSYKDNKQNKGIIKGTLSVVR"
     gene            complement(5637..6023)
                     /locus_tag="PLI16_02425"
     CDS             complement(5637..6023)
                     /locus_tag="PLI16_02425"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOH83444.1"
                     /translation="MALNFFNRRNILKQLNYLEATPIRKCACETAENGLVTLVIPKFR
                     NEKVNNFLLSPRKRFFRIALDELGTSVWQHIDGTKKVMEICQQVYDIYGEKIMPVDQR
                     VTRFLTMLYEGKYISFREIEKDSSGQ"
     gene            complement(6038..6931)
                     /locus_tag="PLI16_02430"
     CDS             complement(6038..6931)
                     /locus_tag="PLI16_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH83445.1"
                     /translation="MNKKVSLVLSSGGCRGLAHIGVIEELLNDGYEIASVSGASIGSL
                     IGGIYASGNLRAFREWVCSLDEVDVYDLIDFTLTGQGFIKAEKLFKIIEEFIDCNTFE
                     ELNIPLSVVATDIIKRQEVVFDKGSLIMAIRSSVAIPTVITPVLYKDMVLVDGGVINP
                     IPVNCIQRTEGDLLVVVNVNANIPYQKPEIKTLPPPGREFSFKRKAWNYAKRHFNLLS
                     GEKKSDGTPGYLTVMDKSIDIMQDRICGLTVMQQHPDIDVKVSREAASTFEFYRAYEL
                     IEAGRAAYRLAKQHSGLSENY"
     gene            complement(7069..>8895)
                     /locus_tag="PLI16_02435"
     CDS             complement(7069..>8895)
                     /locus_tag="PLI16_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH83446.1"
                     /translation="IEPLPDTCVLAGTYLSFTVKAFDPDSGVVTLTGNGAPLLVPSSP
                     ADFPQPVQGTGHVSSQFTWQTECSHVKKSPYQMNFKVIDNDYPVQLVDFESMNITVVA
                     PAPENLTATPSGNTIILNWDRSICPNAVGYRIYRRNGYYGFIPGHCETGVPAYTGYSL
                     IASTDTITDTTYTDNNNGAGLVRGLDYCYMVIAYFADDAESYASLEVCTELSKDVPVI
                     TNVSVKNTDAVNGSLWVGWSKPTEFDTVQIPGPYKYLIWRGTGFNPATYYLIDSLANI
                     NDTVYNDTLLNTQNLPYRYRIDFYNDEPGNRFFVGSTANASSIFLMLTPSDNKLKLTW
                     QPDVPWLNDYYVVYKQNPLTTLYDSIAVVTAQTYTDTGLINGASYCYYVKSVGRYTAS
                     GFAFPLVNLSQKACGIPIDNEPPCPPLLTGEVDCENIQNFLQWALPGDACADDVVAYN
                     LYFSPGEGSDYSLIFSTTNLQDTSFLHTGMGSIVGCYYITAVDSFNNQSIPSNLVCFD
                     IDQCYLYQLPNVFTPNGDGSNDEFRPFPYDFVEKINMVIFNRWGTVVFKTEDPDINWD
                     GKDKNSKRDCSVGVYYYVCDVFEKRLGGVQKRSLTGIIHLIR"
BASE COUNT         2540 a         2014 c         1951 g         2390 t
ORIGIN      
        1 ttgacgggat agataatgac aacgacggct ataccgacaa ttaccgcggc tggggtatgg
       61 gcgaaaacga caacaacccc atgggcacca tctcgcacgg tacgtttacc gccggccttg
      121 cttcggccac caccgacaac ggcgccggca ttgcaggaac gggattccaa tgcagatttc
      181 tgccaataaa ggtgtgtaac gccaataact taggcaccat gacgtacgag agcatcgtgt
      241 atgcggcaga acacggctgc agcattataa actgctcgtg gggcagcact ttttataccg
      301 ggcaatacgg gcaagatgtt attgactatg ccactatcaa ccggggtgcg ctggttgtgg
      361 ccgccgccgg caacgacaac aacacggctg tgtttttccc tgcttcctat aattatgtac
      421 tgagtgttgc ggccagcgac atcaacgacc ataaggccaa tttttcgaac tatggccatt
      481 ttattgatat catagccccg ggcgacaatg tgtggtcaac aggcatggga ggaatttacg
      541 gcagctcagg aggcacctcg ttttcggcac ccattgtttc gggctgtgca gccttgctga
      601 ggtgcaagta tccttccctt tcggtattgc agattgctga aaaactaaaa gtaacagccg
      661 atgtcataga caccctgccg gctaacctgg cctatcgcga tatgctgggg gcaggcaggc
      721 ttaatatttt cagggcacta aacgattcgg tgctaccttc tgtacagatg tttaatatca
      781 attttgaaaa ctcgcatcat caggcccttg ccgacccggg cgatactgtt tacatcacaa
      841 gcaactttat caattacctg gcgccttcca ccgccaatct tacgataagc cttacctgcg
      901 cttcttccca tgttaccctg cttgattcgg tatttgttgc cggaaacctg cccacctcag
      961 gcaccatcaa caatagttct gatccgttca gcctgctcat aaaacccgga ataacaccaa
     1021 acgaaaatct ggtatgtaag tttacgatta cggataataa ctacagggcc gttcaatatt
     1081 actacatcta tgccaacaag gattatattg atatcgacac caataaaatt gccaccaccc
     1141 tcaccagcga cggggcgctc ggttacgtag acgggaatag cttgcagggc ctgggcttca
     1201 gatataataa cggcgaaacc atgctggctg caggaggttt tattgttggc cgttcgggaa
     1261 cacaggtttg cgatgtgtta tatggtgatc cgggcttata tgaccacgac tttaccgtaa
     1321 aaacatctat ggccagggta tttccaccgg agcaggcaga ttttgaaggc aagggaattt
     1381 ttaatgattc actggcagga acatcaaaga taaatgttga ggttacccag cgtgtccatg
     1441 cctggaaaga accgcctaac gacaagttta tcctgctgga atacaccttg aaaaacaaaa
     1501 atgcctatgc cctttcgggc ctttatgccg ggctgtatgc cgactgggac attgatggca
     1561 gcagcacgaa ccgcgccgat tatgatgccg aaacaaaaac cggctatagt tactcaatct
     1621 caggcagcca atatgccggc ataagcctga tttcgtcagg aacagctaca cattatgcat
     1681 ttgacaatac cggtgaaaat ggcagtataa atatttctga cggctttaca agcagcgaaa
     1741 aatatgttgc attgaaaacc aacagaaatt cggctggcat ttcggggtat ggaaatgatg
     1801 tctcgcaaat gcttagctcc gggccgcata ccttggctgc caacgactcg accgtaattg
     1861 tatttgcatt aatcgccggc gatcacctgg gcgacatcca aagcagcgcc acggcggcac
     1921 atcaggctta tttccacaca gggatagttt ccaatgagcc atcaaccgcc gatgtagtat
     1981 tattgcaaaa ccaacccaac ccgtttaaag aaaacacaag tatcggggtt cttgtaatga
     2041 aaaaaacttt tgtggaattg tcgtattgta atgtttccgg gagtacttcg ggaataattt
     2101 tttccggcac tcttgacaaa ggctttcata cttttaccac cggaaacaat atttcacctg
     2161 gaatttatgt ttatactctt acaggtgatg ggttttgcct gaaaaagaaa atgtgtgtta
     2221 cagaataatt tttacgaaca actgacaatt tcttcacacg caacaaacta aaagagttat
     2281 ttttacgtcc ttattttgta aacacttgtt atgatattaa agattatcaa aatatttttg
     2341 ttattgctgg taatagctgt gctgtctgtt tcatgcgaaa caaagaaaaa ttgtggtggt
     2401 atgaagtacc acaacgatga tgtaaagcgc ggattagccc attaaaaaaa aaagactgct
     2461 gaaaaagcag tctttttttt ttcttgtcag agttatgcgt tttcttttac cggggcattt
     2521 ttcaaggttt cggtaaggaa agcccagaat ttttccacag aaggaatatt gactttttcg
     2581 gccggtgaat gcgggaagtt gatggtgggg ccaaaagata tcatatccca atttttataa
     2641 acaccgccca gcaggccgca ttcgagtcct gcgtgtatgg cgctgacatg aggttttgtg
     2701 ccaaattgtt tttcgtaagc aagcagcatt tcttttaaaa tcggagagtc catattgggt
     2761 ttccatccgg gatattggcc atcaaaaatg ctttcggcac aggccaaaga ataaatgctg
     2821 gcaaacatat tttcgagatc gtccttggct gtgtcaacag agctgcgaag cagggccatc
     2881 acgtcaataa cgccgtcttt tgatttcaca atagccaggt tggtagaggt ttccaccagg
     2941 cctttcatat cctggctcat tctgattacg ccgtttggtg tgccatagat ggcatccagc
     3001 aggttgttgg ttgttacatt gtcgatgatg gttttgggca attccacaga ttctgccaga
     3061 atcgtgatat tgtcttccac atttttatat tcgttgatta ctatttcagt aaactcttta
     3121 ataaactgtt tgaatttatc cacgttttct tttggcaggg taaccacggc aaaagattca
     3181 cgcgggatgg cattgcgcag gcttccgccg tcgatgctgg aaagccgcat tcccaggtta
     3241 ttataagcat accataagat acggttgagc agtttgttgg agttagccct ttcgaggtgg
     3301 atatccacac cggagtgtcc tcctttgagt cctttcacag atattttcaa ggcttccaca
     3361 tttccggcca ccggtttttc gtcgtagtaa aatttcatgt ttgcgttggt accgccggca
     3421 caacccacgc agagatcgcc ttcttcttcc gtatcaaggt tcagcagaat atcgcctttc
     3481 agcaggcctt ccttgaggcc gaaggcgccg gtcatgccgg tttcttcatc aacagtaagc
     3541 agcatttcga gggggccgtg ctgcagatcg gtagcttcaa gcactgccat agcggcagcc
     3601 atgcctatgc cgttgtcggc gccgagggta gttcctgttg ccgttaccca gtcgccatca
     3661 atgcgtggtt ttatcggatc ggtagtaaaa tcgtgctgaa tatctgaatt ggcctgggga
     3721 accatatcaa gatgcgcctg caggataatt cctttgcggt tttccatgcc gggggtggcg
     3781 ggtttgcgaa caataacatt tcccgtatcg tctactgaac attcgagttg gtgagttttt
     3841 gcccaggcca gaacgtgttc tcttagtttt tcttcatgtg ttgaagggtg aggaatgctg
     3901 cagatgtcct caaaattttt ccataatgca gtcggtttca aattacttag tacatttccc
     3961 atattcatat aatttttaaa agttagtaac taattttgca gaacaacaaa tataacaatt
     4021 aatacagaac ttttatttta ataaggtttt tttcacaaaa aaataaaggc caaacaacct
     4081 ttttaatatt tttattgtct aaaacaatcg tagatgagcc ttgacagaaa tatcattgag
     4141 ggatgcattg ccggcaacag gaaagcgcaa aaattcctct ttgaaaaata taaggcggcg
     4201 atgatgggcg tttgtttgag gtattgtaaa agtaaagatg aggcagaaga tgtgttgatg
     4261 gaagccttta tgacggtgct gtcgcagata cagtcgtacc gtggcgatgg atcgatagac
     4321 caatggatac gaaggatagt ggttaacact tccatcaaca actaccggaa aaacctaaaa
     4381 cattatttcc atgccgacat cgaaaacatt gccgaaactg agattgaaga ggataacact
     4441 tttgatatca ccgacaatca ttctgtggag gagattatga atgccatgca acaaatgccg
     4501 cagggatata aaatagtgct gaacttatat gtggtggaag ggtataaaca taaagaaatt
     4561 gccgaaatgc tgaatatcac agtaggtacc tcaaaatcgc aactatcgaa agctcggaaa
     4621 attatacaag acaaattaac gaataagtaa gtgtacaaaa taatatgaat gagtttttaa
     4681 ataacggtcc gaaaaatttt gatgattacc tgagaacagc cctggataat tatcagcctg
     4741 atgtttcgtc aggcgtatgg cagtcgctca aactgcagtt atttaaaaag gacgctatag
     4801 attttttaac ttttaaaaaa ctcagccgtt cgttcaattc ctccgcaaac ttcggtgccg
     4861 cccagatcaa agtgctggct acgtatgccg ctgcagcatg tctggcagta ggagttgtat
     4921 atggcgccag cagaatttat aaaagcatcg ttactgttcc tgtcgaaaaa aactccacca
     4981 cagaaccgca aaccaatatc tccacagaag ccggtatccc cggtaatact gcagacaccc
     5041 aagtagcggt cgaagaacat tcaaattcaa gtattccggg aaacaatcgt ttcaaccgca
     5101 ccgtagtgtc atcaggtgcc gaaaatgaag caggccaggc ggttcaggcg gccacagata
     5161 atggcccgga caatagcatg ccggccacat ctcaaaaagc agaaaatgcc gaaacacata
     5221 cgtcactgca aaattatatt gaaaaggtaa cctcccagga taagccatcc ggcaaagaaa
     5281 ccgtagtgca aaacttaccg gaaaccatag atattcctga gacacctgtt gaagagatgg
     5341 ttgaaaatga aaccgaccct tatgcttata atctggaaat acccaatgta atcacgccta
     5401 atggcgatgg ttataatgac ttatttaaaa tcaggaatct cgacaaatat accaacaata
     5461 cgctgaccat tgccaaccga aacggccagg tggtttttga aaccaacaat tatcagaaca
     5521 actgggatgc ccaaaacctt gacgcaggca cgtattatta catattgtct tacaaagaca
     5581 acaaacaaaa caagggaatt atcaaaggaa ccttgtcggt ggtgcgctga ttgtgattat
     5641 tggcccgatg aatctttttc aatttccctg aaagagatgt atttcccctc gtacagcatc
     5701 gtgagaaacc gggttacccg ctggtcaacc ggcattattt tttctccata aatatcataa
     5761 acctgctgac agatttccat aacttttttc gtaccatcaa tatgctgcca cacggaggtg
     5821 cccagttcgt ccagagcaat tctgaaaaaa cgcttgcgcg gactgagcaa aaagttgttc
     5881 actttttcgt tcctgaattt cgggataacc agtgttacca agccgttttc ggctgtttca
     5941 caggcgcact tcctgatggg tgtagcttcc agataattta gttgcttcag tatgtttctt
     6001 cgattaaaaa aattcaatgc cactttttat aacttaatta ataattttca ctcagtccgc
     6061 tgtgttgttt tgccaatctg taagccgccc gccccgcttc tatcagttcg tatgccctgt
     6121 aaaactcaaa ggtagaagcc gcctctctcg acactttgac atcgatatcg ggatgttgct
     6181 gcatcaccgt gaggccgcat attctatcct gcattatatc aattgacttg tccattacgg
     6241 tgagatatcc cggcgtgcca tcggattttt tttcaccgga caacaggttg aaatgccttt
     6301 tcgcgtaatt ccatgctttg cgtttaaaag aaaactctct ccctggcgga ggcaatgttt
     6361 taatttccgg cttctgataa ggaatattgg cattgacatt taccacaacc agcaaatcac
     6421 cctctgtacg ctgtatgcag ttgacaggga taggatttat tacgccgcca tcaaccaaca
     6481 ccatatcttt gtacaaaacg ggggttatta ctgtagggat agccactgat gaacgtatgg
     6541 ccatgatcag gctaccctta tcaaacacca cttcctgccg ttttataata tccgtggcaa
     6601 ccaccgatag cgggatattc aattcttcga atgtattgca atcaataaac tcttcgataa
     6661 ttttaaaaag cttttctgct ttaataaagc cctgccctgt cagcgtaaaa tcaatcaggt
     6721 catacacatc cacttcatcc agcgagcaaa cccattccct gaaagccctg agatttcctg
     6781 aagcatagat accacctatc agcgatccaa tggaggctcc tgatacggat gctatttcgt
     6841 acccatcatt caataattct tcaatgacgc caatatgtgc gagacccctg cacccgccac
     6901 ttgacagaac taaggaaact tttttgttca taatgtaaaa ttaaaaaaaa atcggcttta
     6961 cacgacaaaa atattttaca caatgccatg tctaatcaaa caagtaggaa gatgtgcgaa
     7021 aatctaaacc atccagcgca acaaaaagtc ccggaagggc tgccggattt accttatcag
     7081 atgtatgatg cccgtgagcg atcgcttttg gactccgcca aggcgttttt caaaaacatc
     7141 acagacataa tagtaaaccc caacactaca atcgcgtttg gagtttttat ccttgccatc
     7201 ccaattaata tcggggtctt cagtcttaaa aaccacagtt ccccagcgat taaagattac
     7261 catattgatt ttttcaacaa aatcataggg gaacggcctg aactcgtcgt ttgatccatc
     7321 gccattgggc gtgaacacat tgggaagctg gtacaaataa cactgatcga tatcaaaaca
     7381 tacgaggttg ctgggtatac tctggttatt gaaggaatcc acggctgtaa tataataaca
     7441 cccgacgata gaccccatac ccgtatgaag aaaactggta tcctgcaggt tggttgttga
     7501 aaatatcagg gaataatcgc ttccctcacc cggagaaaaa tagagattat aagccactac
     7561 atcatcggcg caggcatcac cgggaagcgc ccattgcaag aagttttgga tattttcaca
     7621 atccacctcg cccgtgagca atggtggaca aggcggttca ttatctattg gtatgccgca
     7681 ggctttttga gagagattca ccaacggaaa agcaaagccg gaagcggtgt acctgcccac
     7741 cgatttcaca taataacaat agctggcacc gttaatcaat ccggtatcgg tataggtttg
     7801 ggctgttacc acggcaattg agtcatagag ggtggtcagc ggattctgtt tgtacaccac
     7861 ataataatca tttagccagg gaacatcggg ctgccaggta agcttcagct tattatcgga
     7921 aggtgttagc attagaaaaa tggaagacgc gttggcggta gatcctacaa aaaagcggtt
     7981 gcccggttcg tcattataaa aatctatccg gtaacgataa ggtaggtttt gagtatttag
     8041 cagcgtatcg ttataaactg tatcgttaat atttgccagg gaatcgatga ggtaatatgt
     8101 agccggatta aagcccgtgc cccgccagat aaggtattta taaggtcccg ggatttgtac
     8161 ggtatcaaat tctgttggtt tagaccatcc cacccacaac gatccattga cggcatcagt
     8221 atttttgaca ctaacattgg taattacggg cacgtctttc gacagttcgg tacagacctc
     8281 caaagaggca taactttcgg catcatcggc aaaataagct atcaccatat aacaataatc
     8341 taagccacgc accagccctg caccgttgtt gttgtcggtg taggttgtat cagtgatggt
     8401 atcggttgac gctatcagac tatagccggt gtaagcaggc actcccgttt cgcaatggcc
     8461 cgggataaaa ccataatagc cgtttcgccg gtatatgcgg tatcccacag cattaggaca
     8521 aatacttctg tcccaattaa gaattattgt gttgccggag ggagtggcgg taaggttttc
     8581 cggggcaggc gccaccacag taatattcat agattcaaaa tcaaccagtt gtacgggata
     8641 gtcgttatct atcactttaa agttcatctg gtaagggctt ttcttcacat gggaacattc
     8701 ggtctgccag gtaaactgcg aacttacatg gcccgtaccc tgcaccggct ggggaaaatc
     8761 ggcagggctg gatggcacca acaacggagc cccgttgcct gtgagcgtga caactcccga
     8821 atcaggatca aaagctttta cggtaaagga caggtaggtt cctgccagga cacaggtatc
     8881 aggcaaaggc tctat
//
LOCUS       DAOSFP010000048         8823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6758_length_8823_cov_4.291058, whole genome shotgun sequence.
ACCESSION   DAOSFP010000048 DAOSFP010000000
VERSION     DAOSFP010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6758_length_8823_cov_4.291058"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="PLI16_02440"
     CDS             complement(<1..787)
                     /locus_tag="PLI16_02440"
                     /inference="COORDINATES: protein motif:HMM:NF040218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BACON domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HOH83447.1"
                     /translation="MRITAGGYGATEVINATGLTAGTTYYVRVYDYTGSSVPPTSYTF
                     TICVTTPTPPQCAVTSFSPASIAHDPTAFSTNITVSANANCAYTVTGVCSWLSVTPAS
                     GYANISGQFTLSCVVQANTSATARTCTFYVNSTPFTVTQSGCAYNLSLASNSVSASGS
                     TYNLSISTSSPCAWTVANPCSSWVNISQSSGTGTGNISLTVSPNTSCSTRSCTLTVTP
                     GNATHTITQLANTPPTVSVSPANPFFCEGNGPVTITASGATLYN"
     gene            complement(696..>1175)
                     /locus_tag="PLI16_02445"
     CDS             complement(696..>1175)
                     /locus_tag="PLI16_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83448.1"
                     /translation="DVLSSCSLAGSHGYGTKTWQQILSHYYPNWTLASCGSVAPPSNN
                     NCSGAQTLTVYPTSACGGATTGTISGATQSIAPLACAGYTSTLCTDVWYKFTATTTAH
                     TITVVPSSGMDAVVDLRSGACNGSNIACADNGGGIRRHGGDKCHRANSRHDILCQGL"
     assembly_gap    1176..1275
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1276..1487)
                     /locus_tag="PLI16_02450"
     CDS             complement(<1276..1487)
                     /locus_tag="PLI16_02450"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOH83449.1"
                     /translation="MVNYETYCKRVLPQEWYNCWGSLTNGMNSLQAGAVAIRSYTIPR
                     MANYVSSHGGGTYDICTTTCCHVYGTT"
     gene            complement(1648..2382)
                     /locus_tag="PLI16_02455"
     CDS             complement(1648..2382)
                     /locus_tag="PLI16_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83450.1"
                     /translation="MKKLFFFFFAIFFIVSGSRAQGHISASNILNLRFLDVSTGYFVI
                     PDEVIITQRENKDFAMTITSEKISENGTISISFMNGTYDITVNAPNYFSMSTFFQLTN
                     DTLNVNFNLEPIAPLNELTDTYIQSLQAQDAVVITGVIVDEISGKPLNHVKVYLKDNT
                     ASAFSKENGYFVLSAPLPEKEAEIADRNILIFEKDDYITEIRKNFDMWPYGDAIIQIR
                     MNKGTGTHEADIILSRQSVVTLKTPS"
     gene            complement(3018..4046)
                     /locus_tag="PLI16_02460"
     CDS             complement(3018..4046)
                     /locus_tag="PLI16_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83451.1"
                     /translation="MKKINIIICTVVALMPLLSKAQNKMESTFELRYNQTSKLYEAHL
                     LVGGTLNEGGLDNFLGPSGFCIVVPASVADQPIACASHNPVSANWADKTPAYSIATYD
                     GNKDYHKFVTYGQSFIPPLTAGADVLLFTFSLPQDVCPSSVRMFGNNNPSPSPTPDVN
                     PMDLTFDNSFQTLSGETFKTATVNVPLHPVAPVIYQNGAALYTSSTSTIQWYNNNVMI
                     PGVTTTTYTPTVSGNYYIVTTDAYWCTSDTSNTISMVITNIDETEDLSGIETYPNPVV
                     DELVITNNHFKKPIDFEIYNAIGEVVYKGVLKHKTKVDTKGYAAGAYLLKLNNGKKIE
                     SREFIKEY"
     gene            complement(4337..7111)
                     /locus_tag="PLI16_02465"
     CDS             complement(4337..7111)
                     /locus_tag="PLI16_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83452.1"
                     /translation="MLKAQTMQSFLEIKYNQTSCLYEAHLHTQGNITPIDNLLATSGF
                     CIVVPASVPNTPITCTSLSPGTWTDNTPVYNVQTFDGIKDYHKFATMGAQFNPPLYAG
                     ADIILFTFSLPNSVCPDGVRMFENNSPSPSPTPDTNPPGLTFDNSLQTLTGETYTGNT
                     GSAVALPMPQITASCAFSCNLSTLYLYADGNTSLGCNTLTYQWSGPGGYTSSQQNPTA
                     NPFTVPGNYIVTVTDVNNCSATSSVPINNQPLTLQATIASITHVACYGQHTGSISATA
                     SGGNPPYSYTWNTTPVQNSATAQNLAAGTYSVTITDAMGCSSTATTKISQPASALSSS
                     ATVVNTSCQNANGSVNLTVTGGSDPYSFIWSNGITSEDLTNVAAGTFSVTITDSGNCQ
                     VTNTAVVINNGNPPLTPVITQHGDTLCSSATTGNQWYNNNGLITGEIYSTILPGTPGN
                     YYVVVTNNENCVSGPSNIISYPAGNGHTISGKTRYVAKANPGNPFPNQPTYNPVKYNI
                     NKVVVKLKTSPGGAELARDTSDASGIYSFANVPDGNYILSYDKYTADTMQMGNDINSI
                     DVAIALYFIGHDTLLDPTRNFSAKHKKAINVDNNSNINSIDIARLKSKIGQPNLPAAN
                     FPKGNWVAIDTMVTVAGANLNITLQTICYGDFNASSYKYKDSTYTWSQAKSWTKDNII
                     IQEPEALVVGQGAYFEVPLRITSAIDDFSAMDLSLTYPDENFFLTNAYMPRASKSSIP
                     LKINPTLEEVIAGNNDLLVTDTEGNIRVVYATTDFFDVYAQEEIIVLCFRARHDDKFE
                     ETVFALNGSGLLADQYGTEKENVYLSVPEIKMQKINTETKIELSAYPNPFNDNSSIYF
                     NIPESGTVSLRVFNAEGRQVAEVLNNNFDAGNHSVIFSQKNLPHGLYFFRLDFTGNKL
                     SESVSLKMMR"
     gene            complement(7321..7914)
                     /locus_tag="PLI16_02470"
     CDS             complement(7321..7914)
                     /locus_tag="PLI16_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOH83453.1"
                     /translation="MSNEEYSGIKFNLVFEEKTTPHHPLLDELKKWCAVFHEKGLAPP
                     YTGGSYGNLSFRTSNNSFIITGTCIGLKNALDNSCFVEVISCDMARKEVIVKGMRAPS
                     SESFMHHILYQSRPDIQAIFHGHNPLIIQHAGLLGISETTKKTSYGTTELAKCVKEIA
                     IDNDFIVIKEHGFVSMGATMQQAGEQTLHWLDKAALI"
     gene            complement(7914..>8823)
                     /gene="mtnA"
                     /locus_tag="PLI16_02475"
     CDS             complement(7914..>8823)
                     /gene="mtnA"
                     /locus_tag="PLI16_02475"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOH83454.1"
                     /translation="KTMIVRGAGAIGVTAGYAMAQAFMESNPYNFEEYITAAKIQIEA
                     TRPTARNLFYAVERVYNAGKKSIAHAITEAEKLALEDVESSKKIGKFGNTLIAHNSRI
                     ETHCNAGWLAFTDFGTALSPVYAAAHAGKKIFVYVDETRPRSQGARLTAWELQNENIP
                     HTIIPDNAGAYLMSQGKIDMVIVGADRIAANGDTANKIGTLEKAIAAHAYGIPFYVAA
                     PMSTFDRYCSEGKNIPIEERSPDEVLYQKGPDKNGYMHEILVCSPGSSALNPAFDVTP
                     AKYITKIITEKGIINPDTTEIKKLFT"
BASE COUNT         2273 a         1694 c         2150 g         2606 t
ORIGIN      
        1 agttgtataa tgtggcgcct gaggctgtga tcgttaccgg tccattgccc tcgcaaaaga
       61 acggattggc aggactgaca gataccgtag gcggcgtgtt ggccagttgt gtaatcgtat
      121 gagtggcatt accgggagta acggtgagag tgcatgagcg tgtactgcac gaggtgttgg
      181 gagagaccgt cagggaaatg ttccctgttc cggtaccgct gctttgactg atgttgaccc
      241 atgaagagca tggattggca acggtccagg cacatgggga tgatgtggag atagataagt
      301 tatatgttga gcctgatgca gaaacagaat tggaagcaag ggacagattg taagcacagc
      361 cgctttgggt aacggtaaac ggggtgctat taacgtaaaa ggtacatgtt cgggcagtag
      421 cactggtatt tgcctgaact acacaggaca aagtgaattg accggaaatg ttcgcataac
      481 cactggcggg tgttacgctt aaccaggagc ataccccggt tacggtgtag gcgcaatttg
      541 cattggcgct tacagtaata tttgttgaaa acgcagtagg atcatgggct atggatgccg
      601 gagaaaagga tgtaacggca cattggggag gtgtgggcgt tgttacgcaa atggtaaaag
      661 tatatgaggt gggaggcact gaagatcctg tataatcata aaccctgaca taatatgtcg
      721 tgccggctgt tagcccggtg gcatttatca cctccgtggc gccgtatccc cccgccgtta
      781 tccgcacagg cgatgtttga gccattgcag gcaccggacc ggaggtctac tacagcatcc
      841 atgcctgaag atggaaccac agtaatggta tgggctgttg tcgttgctgt aaatttatac
      901 caaacatcag tacacagtgt acttgtatat ccggcgcatg cgagtggggc tattgattgc
      961 gtagcgcctg aaatggtgcc tgttgttgca cctccgcaag cagacgtggg ataaacagtc
     1021 agcgtttgtg ctccggagca gttattattg gaaggcggcg caacagagcc acaactggcc
     1081 aaagtccagt tgggataata atgactaagt atttgctgcc atgttttggt tccgtaacca
     1141 tgagacccgg ccagactaca cgaagatagc acatcnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnngtggt gccatataca tgacagcagg tagtggtgca aatatcataa
     1321 gttccccctc catgcgatga aacataattg gccatgcggg gaatggtgta cgaccggata
     1381 gccactgctc ctgcctgcaa cgaattcata ccattggtaa gagaccccca gcaattatac
     1441 cattcctgag gaagaaccct tttgcagtag gtttcgtaat ttaccacatc aatggtgccg
     1501 gtgcatgtat gacatgaaca actgctgccg actttgatag tagttggaca agattagccc
     1561 attagttcaa ataacgtata aaaagacaag ataaggtaca aatatgtttt tttcatggtt
     1621 gatttttgtt gctgtttttg tttattttta cgaaggtgtc tttagtgtaa ccaccgattg
     1681 cctcgagagg ataatatctg cttcatgagt tcctgtgcct ttattcattc ttatttgtat
     1741 tatagcatct ccgtatggcc acatgtcgaa gttttttctg atttctgtga tgtaatcatc
     1801 tttttcaaat atcagaatat tccggtcagc tatttctgct tccttttcag gtaaaggggc
     1861 gctaagtacg aaatatccgt tttcttttga aaacgcagat gctgtattgt ctttaagata
     1921 aactttgaca tggtttaatg gtttcccgga aatttcatct acaatgactc ctgtgataac
     1981 aacagcatct tgtgcctgta aagattgaat gtaggtgtct gtcaactcat ttaagggggc
     2041 tatgggctct aaattgaaat tcacattcag ggtgtcattg gtcaattgga aaaatgtgga
     2101 catcgagaaa taattcgggg catttacagt aatatcataa gtcccgttca tgaacgaaat
     2161 tgaaattgtc ccgttttccg atattttctc tgaagttatt gtcatagcaa aatccttatt
     2221 ttctctttgg gtgattatca cctcatcagg aattacgaaa tacccggtgg aaacatccag
     2281 aaaacgcagg ttcagtatgt ttgaggcaga aatatgtccc tgggcccggc ttcctgatac
     2341 tataaaaaat attgcgaaaa agaagaaaaa cagttttttc atgatggttt tttttacacg
     2401 attattttca aaaaatcaat aaaacttttt tgtacaaaaa aacagaaaaa aaatggtttt
     2461 cgggtaagaa atttttgagt ttatcataca tttaaaacga tttttagact acacgtaagg
     2521 tgcaaaaagg gcgcattctg cccataatcc aaaaccttat actcattagc acattctttc
     2581 ctatagtgtt gggccctgca aggaaagaag ggatcatatg tgactggtgt ttgctgcgtg
     2641 aaacactttg tgtttttttg ttatgtttgg attatggatc cgcagtgatc tgtcctgact
     2701 acccggtcag gtggaatatt ctggactata aaacggggca gagacaagaa tgtttctacc
     2761 aatttttatt tgaaaggaac ctgaccgaac acgaattgtc aggactggac ttgcttttta
     2821 gaacactcca agaaatgata ctgatttgaa agatcaaaat acagcccggt tgtcccggcg
     2881 tatttgattc tgagtttaga gcttcgaaaa caatggattc cctgatttca cgtagtgtgg
     2941 atgtataaaa gaatgacttt agagccggct ctttatttta caaagagaaa tgttatagat
     3001 tgaaaatgga tagccggtta atattctttg ataaattccc gcgactctat cttcttgccg
     3061 ttgttaagct tcaataaata agcgccggca gcatacccct ttgtgtccac tttggttttg
     3121 tgttttaaca cccccttata gacaacttcg ccgatagcat tatatatttc gaaatcaata
     3181 ggttttttaa agtggttatt agttatgact aattcatcca ccaccgggtt gggataggtt
     3241 tcaatgccgc tcaaatcctc tgtttcatca atattggtga ttaccatact gatcgtgttg
     3301 gatgtgtcgg acgtgcacca gtaagcatcc gtagttacta tgtaatagtt gccggaaacc
     3361 gtgggtgtat atgtggtcgt cgtcactccg ggaatcatta cgttattatt gtaccattga
     3421 atagttgatg ttgatgaggt gtataaggca gcgccgtttt gataaatcac gggtgcaaca
     3481 gggtgcagtg ggacatttac agtagctgtt ttgaatgttt cgccgctaag cgtctgaaaa
     3541 gaattatcaa aagtaagatc catggggttc acgtcggggg tgggtgatgg cgaaggatta
     3601 ttattgccaa acatccgcac actgcttgga cacacatcct ggggcaggct gaaagtgaac
     3661 agaagcacat cggctccggc agtaagcggc gggataaacg actgtccgta agtaacaaat
     3721 ttatgataat ctttgtttcc atcgtaggtg gcaatactat aggccggcgt tttatcagcc
     3781 caattggcgc ttacggggtt gtggcttgca caggcaatag gctggtcggc taccgaagcc
     3841 ggtaccacaa tacaaaaacc ggaaggccct aaaaaattgt ccagtccacc ctcgtttaag
     3901 gtgccgccta caagcaaatg ggcttcgtaa agcttgctcg tctgattata tctcaattca
     3961 aatgtggatt ccattttgtt ttgagctttt gacaggagag gcatcagcgc aacaactgta
     4021 caaattatga tatttatttt tttcatattt tcatgaattt tgaaaacatt tttttgttta
     4081 tcactatctc ttttagagat aattatgaac aaatatacga taatttatct gaaaagttat
     4141 gaaattttat aaaaatttca cttttgcctg caatggaaaa aataatggtc atcaaatttt
     4201 gccttaacga attgattttt ttacagtttc cggctcaggg ccattgtcat gcctcatttc
     4261 agggctatgt ttggcgcctg taaaacattc tgaattatga aaaacgtgcc gttatcacaa
     4321 aacccggtac aggctgttac cgcatcattt ttagtgatac gctttcggac agcttgtttc
     4381 ccgtaaaatc aagcctgaaa aaatacaatc catgaggcag gtttttttgt gaaaaaatca
     4441 cggaatggtt accggcatca aagttattat tcaacacctc ggcaacttgc cgtccttcgg
     4501 cgttgaatac ccgcagagac accgttccgg attccgggat attaaaatat atggatgagt
     4561 tgtcattaaa gggattggga taggccgaca attcaatttt ggtttctgtg ttaattttct
     4621 gcatttttat ttccggtacg gaaaggtaca cattttcttt ttcagttcca tattggtctg
     4681 ctaacaagcc tgagccgttc agggcaaaaa cagtttcttc aaacttatcg tcatgccggg
     4741 cacggaaaca tagtacaatt atttcttcct gcgcatacac atcaaaaaaa tccgtggtag
     4801 cgtaaaccac cctgatgttc ccttcggtat ctgtaaccag cagatcgttg ttgccggcaa
     4861 ttacttcttc aagggtgggg ttaattttca acgggatgct actcttactg gcccggggca
     4921 tataggcgtt cgttagaaaa aaattctcat caggataagt cagggacaaa tccatagcgg
     4981 aaaagtcatc aatagcagag gtgattctca acggaacttc aaaatatgcg ccctgtccaa
     5041 ccactaaggc ttcaggctcc tgaatgatga tattatcctt agtccatgat tttgcctgac
     5101 tccaggtgta ggtcgaatct ttgtatttat agctggatgc attgaaatct ccgtaacaga
     5161 tggtttgcag ggtgatgttc aggttggcgc cggcaacagt aaccatggta tcaatagcca
     5221 cccaattccc ttttgggaag ttggctgccg gaagattggg ctgtcctatt ttggatttta
     5281 gccgggcaat gtcaatagaa ttgatgtttg aattattatc tacattaatg gctttcttgt
     5341 gttttgccga aaagtttctg gtaggatcca gcagggtatc gtggccaata aaatataatg
     5401 cgatggccac atcgattgaa ttgatgtcgt tgcccatttg catggtatcg gcggtatatt
     5461 tatcatagga aaggatgtaa tttccgtcag gcacattggc aaaggaataa atacccgaag
     5521 catcactggt gtctcttgcc agttcagcac ctcccgggct ggttttaagt tttacaacaa
     5581 ctttgtttat gttatacttt accggattat aggtcggttg gttgggaaaa ggattgccgg
     5641 gattagcttt ggccacgtag cgggtttttc cgctgatggt atgcccgttt cctgcaggat
     5701 aagagatgat atttgatggg ccggaaacac agttttcatt gttggtaaca accacatagt
     5761 agttacccgg ggtgccgggg aggatagtgg agtatatttc acctgtgatg aggccattgt
     5821 tgttgtacca ctggtttcct gtggtggccg aagaacacag ggtgtcaccg tgctgtgtaa
     5881 tcaccggagt caaaggaggg ttgccgttgt ttattaccac ggcagtgttg gttacctggc
     5941 agttgccgga atcggtaatg gttacactga aggtaccggc ggctacatta gtcaaatctt
     6001 cgctggttat accgttcgac cagataaaac tgtagggatc actgcctccc gttaccgtca
     6061 gatttaccga gccgttggca ttttggcagc tggtgttgac aaccgtggcc gaagaagaca
     6121 atgcgctggc gggttgtgaa atttttgtgg tcgctgtgga agagcaaccc atggcatcag
     6181 taatggtaac ggagtaggtt ccagcggcca ggttttgtgc cgttgctgaa ttttgaacag
     6241 gtgttgtgtt ccatgtgtaa ctgtaaggag gatttccgcc cgaggcagtc gccgagatac
     6301 tccctgtgtg ttgaccataa caggccacat gggttatcga agcaatggtt gcctgaagtg
     6361 tgagtggctg attgtttatc ggcacactgg aagtggcaga gcaattgttg acatcggtaa
     6421 ctgttacgat atagtttccg ggaacggtaa atggatttgc cgtcggattt tgttgtgagg
     6481 aggtgtaacc acccggtccg gaccattggt atgtcagcgt gttgcaaccc aggctggtgt
     6541 tgccgtcagc gtataggtat aaagtgctga ggttgcagga aaatgcgcaa gaggctgtta
     6601 tttgtggcat aggcagagca acagcactgc cggtattacc tgtataggtt tcgcctgtaa
     6661 gtgtttgtaa cgagttgtcg aatgttagac cgggagggtt ggtgtccggt gttggcgaag
     6721 gcgatggtga gttgttttca aacatcctga caccatcagg gcacactgaa tttggcagac
     6781 tgaaagtaaa taaaatgata tcggctccgg cgtacagagg cgggttgaac tgcgcgccca
     6841 tggtggcaaa tttgtggtaa tctttaattc cgtcaaaagt ctgtacgttg tacacaggag
     6901 tattatctgt ccaggttccg ggactaaggg atgtgcacgt tatgggcgta ttgggtaccg
     6961 aggctggcac tacaatacaa aaacccgatg tggccaacaa attgtcaata ggtgttatat
     7021 tgccctgggt gtgcaggtgg gcctcataaa ggcagcttgt ctggttgtat tttatctcca
     7081 gaaaagactg catggtttgt gcttttaaca tagtagatat tgttaaaaga aaggtaatta
     7141 ttatggtggg tattttcatg aaaaacgtct attaattgat taaaaatgaa tataaaacaa
     7201 tgtaaggcaa agataagaaa atattattaa aaaacaaaac tgtttttgta gatattatgc
     7261 cggtaagttt ttatgtgctt tttggggaag tgtagtaccg ggataaggcg aggagttttt
     7321 ttagataagg gctgctttgt caagccagtg caaagtttgc tctccggctt gctgcatggt
     7381 ggcccccatg gaaacaaaac cgtgttcttt tatcacgata aagtcgttat caatagcaat
     7441 ttcttttacg catttggcca gttcagtggt tccataagat gttttttttg tggtttcgga
     7501 tattcccagg aggccggcat gctgaatgat gagtgggttg tgaccgtgaa aaatggcctg
     7561 gatatccggg cggctctggt acaggatatg gtgcataaaa ctttcggagg aaggtgctct
     7621 cattccctta acaattactt cttttcgagc catatcacag gatattactt ccacaaaaca
     7681 tgaattatcc agggcgtttt ttaatccgat gcatgttccg gttataatga aagaattgtt
     7741 agaagttctg aagctgaggt ttccgtaaga tcctcctgta tatggggggg ccaggccttt
     7801 ttcgtgaaaa acggcacacc actttttcaa ctcgtcgagc agggggtgat gcggtgtggt
     7861 tttttcttca aaaaccagat tgaattttat gccgctgtat tcttcattgc tcatcaggtg
     7921 aaaagttttt ttatttctgt tgtatcagga ttgatgatgc ctttttcggt aattattttc
     7981 gtgatgtatt tggcgggtgt tacgtcaaag gccggattca gcgctgatga gcccggggaa
     8041 cagacaagaa tttcgtgcat gtacccattt ttgtcggggc ctttttgata caatacttca
     8101 tcgggactgc gttcttctat aggaatgttt ttgccttcgg aacaataccg gtcaaatgtt
     8161 gacataggtg cggccacata aaatggtatg ccataagcat gcgcggcaat ggctttttcc
     8221 agtgttccta ttttattggc cgtgtcgccg ttggcggcaa tacggtcggc accaacaatc
     8281 accatatcaa ttttgccctg cgacatcagg taggcaccgg cattgtcggg aatgatggtg
     8341 tgaggaatat tttcgttttg taattcccag gctgtaaggc gcgctccctg actgcgcggc
     8401 cgtgtttcgt ccacataaac aaaaatcttt tttccggcat gagcggcggc atacaccggg
     8461 gatagcgccg tgccaaaatc agtgaaggca agccatccgg cattgcaatg ggtttcgatg
     8521 cgcgaattgt gggcgattaa tgtgttgccg aatttaccta tttttttgct ggattccaca
     8581 tcttccagtg ccagtttttc tgcttcggtt atggcatggg cgatggattt ttttccggca
     8641 ttatacacgc gttccaccgc atagaacagg tttcgtgccg tggggcgggt ggcttcaatt
     8701 tggatttttg cagcggtaat gtattcttca aaattgtacg gattactttc cataaaagcc
     8761 tgagccatgg catagccggc ggtaacgcct atggcgccgg caccgcgcac tatcattgtt
     8821 ttt
//
LOCUS       DAOSFP010000049         8821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6762_length_8821_cov_4.303901, whole genome shotgun sequence.
ACCESSION   DAOSFP010000049 DAOSFP010000000
VERSION     DAOSFP010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6762_length_8821_cov_4.303901"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLI16_02480"
     CDS             complement(<1..328)
                     /locus_tag="PLI16_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83455.1"
                     /translation="MIKKILFSLVAFALCIHAEAQWTNKTMVFETLSRSYRVYKSPNY
                     NPSVPASLVITLHGLGDNMTNFSQLGFNYIADTANIIVVCPQAVNDPLVAAVFGYGTA
                     WNSGAGM"
     gene            546..1541
                     /locus_tag="PLI16_02485"
     CDS             546..1541
                     /locus_tag="PLI16_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH83456.1"
                     /translation="MQEIIDIRELNDRIQRESAFVDMVSMEMSKVIVGQKHMVERLLI
                     GLLSNGHILLEGVPGLAKTLAIKSLASAIDAKFNRIQFTPDLLPADLVGTMVYSQKKE
                     EFNVRQGPVFSNFILADEINRSPAKVQSALLEAMQERQVTIGDTTFKLPEPFLVLATQ
                     NPIEQEGTYPLPEAQVDRFMLKVVIGYPSKEEEKLIMRQNIGNKTATTSPIIKTDDII
                     KARAIVREVYIDEKIENYITDIVFATRYPDKYNLKKFQGLISYGASPRASIYLALAAK
                     AYAFIKRKGYVYPEDIRAVCHDVLRHRIGLTYEAEAENITTEEIINEILNSVEVP"
     gene            1932..2807
                     /locus_tag="PLI16_02490"
     CDS             1932..2807
                     /locus_tag="PLI16_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOH83457.1"
                     /translation="METADLLKRVRKIEIKSRGLTNQIFSGQYHSAFKGRGMAFSEVR
                     EYNYGDDIRSIDWNVTARFDHPYVKVFEEERELTVMLLIDVSGSNYFGTQQMLKTEKI
                     TEIAAVLAFSAIQNNDKVGVVFFSDRIEKFIPPKKGTAHILRIIRELVTFKSQSCGTK
                     LSEPLRFLTNAIKKRCTAFLITDFMDTGFQDAIKIASRKHDLIGIRIVDKRESELPRA
                     GLMKIRDFESNQTYWVDTSGKEFQETHKYRWQQHEKMLHDIFVKSRIDVAKVNTDEDF
                     TKPLIKLFKKRESRY"
     gene            2791..3828
                     /locus_tag="PLI16_02495"
     CDS             2791..3828
                     /locus_tag="PLI16_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83458.1"
                     /translation="MNQDIKGMKPGLRLSDFFVILLLIGLPAAVFAQKPEAVASIDSN
                     NILIGDQVKYSYKFTFPSAATPLMPVIADTLTKEVEVVSRTKMDTAVSADKKFTTFSQ
                     TLYISSFDSGTFVIPPMVFSYRQANDTTTYYASTNPIMLYVNTVSVDTTKEFKDIKMP
                     LGEPLTWREILPWALLGFAVLLITGIVIYVWRKLRRKEPIIQLPQKPKIPPYEKALTE
                     LEALRIQKLWQSGKTKEFHSGLTDILRTYMEGQFQVEAMEMITSDVIASLKAAQLPQE
                     EITRMEKILIMADMVKFAKFNPLPDEHDQSLKNGVLFVENTRPKANATAQAGTTIEPN
                     SQKPQEEPKTE"
     gene            3832..4836
                     /locus_tag="PLI16_02500"
     CDS             3832..4836
                     /locus_tag="PLI16_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH83459.1"
                     /translation="MLLSILYIYLAQPWYLLLLIIIPLLIIHYFYKHKNSTSDIGYSS
                     AAGIENIPPTLRQRLIHLPFILRMLLIALLIFIIARPQSSLDKSDIRVEGIDILISLD
                     ISGSMLAEDFKPNRLEASKEVAMQFIDGRPDDRIGLVIFSGESFLQCPLTVDHTVLKS
                     FFSQVKSGMITDGTALGDGLGLSVFHIKKSKAVSKVIILLTDGINNTGSLDPLTAAEI
                     AKKFGIRVYTIGVGKMGKAPYPFQTSFGIQYQNIDVQIDEKLLQQVAEMTGGKYFRAT
                     NKKKLEEIYDEINKLEKSKIDVTRFQNKKDEYFVFILAALGLLVLEIVLRLTVFKKIP
                     "
     gene            4844..5890
                     /locus_tag="PLI16_02505"
     CDS             4844..5890
                     /locus_tag="PLI16_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH83460.1"
                     /translation="MVAFDHLILLSLLILIPVCIVLFLLMLRWKKNTMQKFGDTGVVQ
                     RILTDHSTSRPVVKFVLLMLALAMLVFAVVNMKIGSKMSSVKRKGLDIIIALDISNSM
                     LAQDIKPSRIVRSQMAITKLVDKFEDDQIGIIVFAGKAQVLLPVTTDYAAAKMLASEA
                     NPDIMQAQGTAIGEAINLARVSFDKNSKNKKVIIVISDGENHEDDAIEAARAAANDGL
                     LTYTIGIGSASGAPIPVSMSGFNIEYKKDNSGNTVVTKLNETMLQQVAAAGKGTYVRA
                     TTNDAGLNKVFDEINKLEKKEYEARIFSEYRNTYMYFVIAALLLLIADLIILERKTRL
                     TRNVHLFEKKHIIS"
     gene            5908..6639
                     /locus_tag="PLI16_02510"
     CDS             5908..6639
                     /locus_tag="PLI16_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83461.1"
                     /translation="MTVLHSKPGIKSWCFQLTVIIVLLGITTHVPAQTAVKELRAGNK
                     LFEKNDYKSAEISYRKALEKQNNYQKALFNLGDAMYKQGKYEESEKIFQNLTESDIPK
                     EIAAKAYHNLGNSQLQQKKYQESVESYKQALRQKPNDNETKYNLEYAKRMIQKQQQQQ
                     QQQQQQQNQDQKQQQQQQQQQQQQNKDQKQDKNQQNQQNQQKQQISKEDAERMLNALQ
                     NKEKNLREKMEKEQQNQNYKTEKDW"
     gene            6919..8811
                     /locus_tag="PLI16_02515"
     CDS             6919..8811
                     /locus_tag="PLI16_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOH83462.1"
                     /translation="MKGIITKYRHCALIIVTIVFCNVPGIALAQKFTASASSASAGLN
                     NSIRITYSLSDAQAESFQPPSFSGFNASGPYNSSSSNISFIGGKSVVQSTQSWIYSLT
                     PTKAGKFTIEPAKVKVNGQWISSNSLTIEVANTGTAQGNQNQKQQQQTQQNQQSSTAE
                     ASATDLFVKVFTDKTNVMQGEQLTVTYKIYTRIPVSQYAIQKLPSFSGFWSYDLMKNK
                     EQAVQYNETVNGQKYTVAEIRKVALFPQKSGKLTVDPLEVEALAHILVKKKANNPFGN
                     FFNDPFFNDPFFQNAFTSGYQEVKKTLKSNALSINVSALPAGQPEDFCGLVGNLNMEA
                     TLDKNEVKANDAVTLTVKISGKGNLNLIDNLNIEFPPDFEVYDPQITENISSGNEITG
                     SKTFTYLIIPRTQGNFKLNPITLSYYDKNKKSYATLSSGEINLKVGKGDGSGGAYISS
                     TSKEDIKYIGSDIKFIENKDFEVFPGDYFFFGSAWYWFLLVLPVLLFIAFVILMRRYI
                     KLQSNTALLKNRRATKVALKRLKQANAFMKSNDHNNFYQELSRALWGYVCDKFSIPLS
                     ELSLDTARETLTNKNIEESIRTKFIEILDDCEFARFAPAEATKGMETLYNEAVEIISK
                     TERELK"
BASE COUNT         2908 a         1943 c         1936 g         2034 t
ORIGIN      
        1 acattcccgc tcccgaattc caggctgttc cgtaaccaaa caccgcagcc accaaaggat
       61 cattgaccgc ctgagggcat actacaatga tgttggccgt atcggcaata taattaaatc
      121 ccagctggct gaaatttgtc atgttgtcgc ccagcccatg cagggtaatc accagcgaag
      181 ccggcacgga aggattatag ttcggagact tgtaaacacg gtagcttcgg gaaagcgttt
      241 caaaaaccat agtcttgttt gtccattgcg cttcggcatg aatacacaaa gcaaatgcga
      301 ctaatgaaaa gagaattttt tttatcattt ggattgattt aaaggttttt tgcccgaata
      361 ttttgatgca caaagttata aaagcctgtg tatttaaaaa aattttttcc tgatataata
      421 aatcagaaaa aaatgagttg tatatggtgc ctgtttcagg cttacattaa cattttttaa
      481 caatatttta cttgaaataa tttttgtaag gattttaatt ttgtgcccta aaaaaacata
      541 ccattatgca ggaaataatt gatatcagag agcttaatga tcgtattcag cgcgagagcg
      601 catttgttga catggttagc atggaaatgt cgaaagtgat cgtaggacaa aaacacatgg
      661 tagagcgcct gctcatcggc ttgctgtcaa acggccacat tttgctggaa ggagttcccg
      721 gcctggcaaa aaccctggcc ataaaatcgc tggccagtgc cattgacgcc aagtttaacc
      781 gcatacagtt tacacccgac ctgctccctg ccgacctggt tggcaccatg gtttacagcc
      841 agaaaaaaga agaatttaat gtacgtcagg gccctgtttt ctccaacttc atcctggccg
      901 atgaaatcaa ccgttcaccg gctaaggtgc agagcgcctt gctggaagcc atgcaggaac
      961 gccaggttac catcggtgat accaccttta aactgcccga gcctttcctt gtgctggcta
     1021 ctcaaaatcc tatcgaacag gaaggcactt atcccctgcc cgaagcccag gtggaccgct
     1081 tcatgctgaa agtcgtcatc ggctatccaa gcaaagaaga agaaaaactt atcatgcgac
     1141 aaaatatcgg taacaaaacc gccaccacca gccctatcat taaaaccgac gatatcatca
     1201 aggctagggc catagtgcgg gaggtatata ttgacgaaaa aatcgaaaac tatattaccg
     1261 atattgtgtt tgctacccgc taccccgaca aatacaacct gaaaaaattc cagggtctta
     1321 tcagctatgg agcatcgccc cgtgcaagca tttatctggc gttggccgcc aaagcctatg
     1381 cctttatcaa acggaaaggt tacgtgtatc ccgaagacat cagggctgtg tgccacgacg
     1441 tgttacgcca ccgtattggc ctgacatacg aagccgaagc cgaaaatatt acaacagaag
     1501 agattattaa tgagattctg aatagtgttg aagtgccata ggatggaaag ggaaaagggg
     1561 aaagaggaaa caaggaaatg agttaattaa gttgattgag gaggtgaaga agggagagat
     1621 taggagatta ggagattgag agaattagtt aattaagtta attgagtgaa tgaggaaatg
     1681 gggaagatag ttaattaagt tgattgagga aatgaggaag tgagttaatt gagttgattg
     1741 aggaaatgag gaaatgagag attgagagat tgagagattg agagattgag agattgagag
     1801 attgagagat tgggggatta ggaagtgggg aaagatgata gttttgatga ataacggaat
     1861 cagatggggg attggttcgc aacggcggat cggagcattg gaagataaaa gataaaagta
     1921 gctagttgaa agtggaaaca gcagatttac ttaaacgggt cagaaaaatt gagattaagt
     1981 cgcgtggact aaccaaccag attttttcag gacagtacca tagtgccttt aagggccgcg
     2041 gtatggcatt cagcgaagtg cgcgaataca actacggtga cgacatacgc tctatcgact
     2101 ggaatgttac cgccaggttt gaccatccct atgtgaaggt gtttgaagaa gaaagggaac
     2161 ttacggtgat gctccttata gatgtcagcg gatcgaatta ttttggcaca cagcaaatgc
     2221 tgaaaaccga aaaaatcacc gaaatagctg ccgtgctggc attttcagcc atacaaaaca
     2281 acgacaaagt gggtgtggta ttttttagcg accggataga aaagtttatc ccgcccaaaa
     2341 aaggcacggc ccacatattg cgcatcatcc gcgaactggt tacttttaaa tcgcaaagtt
     2401 gcggcactaa actttcagaa cccctgcgtt ttctgaccaa tgcaataaaa aaacggtgca
     2461 cggctttcct gatcaccgac tttatggata ccggttttca ggatgccata aaaattgcct
     2521 cccgcaagca cgacctcata ggtatcagaa tagtggacaa acgcgaaagc gaactacccc
     2581 gggcaggcct catgaaaatc agggacttcg agagcaacca gacctattgg gtagacacgt
     2641 cgggcaaaga gtttcaggaa acacacaaat accggtggca gcaacatgaa aaaatgcttc
     2701 acgacatttt tgtgaaaagc cgcatagacg ttgccaaagt aaacaccgac gaggacttta
     2761 ccaaaccgct gatcaaatta tttaaaaaac gtgaatcaag atattaaggg tatgaaaccg
     2821 ggattaaggt tatcggattt ttttgtcata ctgctcctca tcgggctgcc ggcggctgtt
     2881 tttgcccaaa agcccgaagc cgtcgcctcc atcgattcca acaatatact tatcggtgac
     2941 caggtaaaat atagttacaa attcacattt cccagtgcag ccactccttt aatgcccgtt
     3001 attgccgaca ccttgaccaa agaagtggaa gtagtgtcgc gcaccaaaat ggacaccgcc
     3061 gtctctgccg ataaaaagtt taccacattt tcacaaacct tatacatttc atcattcgac
     3121 tcggggactt ttgtgatacc tccaatggtg ttctcttacc ggcaggccaa cgacaccacc
     3181 acgtattacg ccagcaccaa tccgataatg ctttatgtta acacagtgtc tgttgacacc
     3241 accaaggaat tcaaagacat aaaaatgcct ctcggcgaac ccttgacctg gcgcgaaata
     3301 ctgccctggg cgttgctcgg gtttgccgta ttgttgatca ccggtatcgt aatatatgta
     3361 tggcgcaaac tcagaagaaa agaacccatc atacagttgc cacaaaaacc caaaatacct
     3421 ccttatgaaa aagccctcac cgagctggaa gccttacgca tacaaaaact ttggcaaagt
     3481 ggcaaaacca aggaatttca ctccgggctg accgatatcc tccgcaccta tatggaagga
     3541 caatttcagg tggaagccat ggaaatgata acttccgacg tcattgcatc gctaaaagcg
     3601 gcacagctac ctcaggaaga aataacccga atggaaaaaa tactcattat ggcagatatg
     3661 gtaaaatttg caaagtttaa cccgctgccc gacgaacacg accaaagcct gaaaaacggc
     3721 gtgctgtttg tggaaaatac caggccaaaa gccaatgcta ctgcgcaggc cggtacaact
     3781 atcgaaccca atagccagaa acctcaagaa gaaccaaaaa ctgaataaga aatgttactc
     3841 agcatactat acatatatct tgcacaaccg tggtatctgc tgttgctgat catcatcccg
     3901 cttctgataa tacattattt ttataaacac aaaaacagca cctctgatat tggttattcg
     3961 tctgctgcgg gaattgaaaa catccctccc acactcaggc agcggctgat acatctgcct
     4021 ttcattctgc gtatgctgct cattgccctc ctgattttca tcattgcacg gccacaaagc
     4081 agcctagaca aaagcgacat cagggtggag ggaatagaca tccttatctc cttagacatc
     4141 tcgggcagta tgcttgccga agatttcaaa cccaaccgac tggaagcgtc caaagaggta
     4201 gccatgcagt ttattgacgg ccgccccgac gaccgcatcg gcctggtaat tttcagcggg
     4261 gagtcatttt tgcaatgtcc gctcactgtt gaccacaccg tactgaaatc atttttcagc
     4321 caggtaaaaa gcggtatgat aaccgatggc actgccctgg gagatggcct gggcctttct
     4381 gtattccaca tcaaaaaaag taaagctgta agtaaagtaa ttatactgct tacagatggt
     4441 atcaacaata ccgggtcgct cgatccgctc actgcagcgg aaattgctaa aaaatttggc
     4501 atcagggtct atactatcgg cgtaggaaaa atgggaaagg cgccctaccc tttccaaacg
     4561 tctttcggca tacagtatca gaatattgat gtgcaaattg atgaaaaatt gcttcagcag
     4621 gtagccgaaa tgacaggagg aaaatatttc agggcgacca acaaaaagaa actcgaagaa
     4681 atttatgatg aaataaataa actggagaaa tcgaaaattg atgttacccg ttttcaaaac
     4741 aaaaaagacg aatattttgt atttatcctg gcagcattgg gactgctggt actggagatt
     4801 gttcttcgtt taactgtttt caaaaaaata ccctgatacg gctatggttg catttgatca
     4861 cctcatatta ttaagcctgc tgatacttat ccctgtatgt atcgtactgt ttttgctgat
     4921 gttacgctgg aagaaaaata ccatgcaaaa gttcggcgat accggcgtgg tgcaacgtat
     4981 cctcactgac cactcgactt ccaggcctgt agtaaaattt gtgctgctca tgctggcatt
     5041 ggccatgctg gttttcgccg tggttaacat gaaaatcggt tcaaaaatga gttcggtaaa
     5101 aagaaaaggc ctggatatca tcatcgccct cgacatctcc aacagcatgc tggctcagga
     5161 tatcaagccc agccgtatcg tgcggtcgca aatggctatc acaaaacttg ttgataaatt
     5221 tgaagacgac cagatcggaa taattgtctt tgcaggtaaa gcacaggtgc tgctgccggt
     5281 aaccaccgac tatgccgcag ccaagatgct ggcctcggaa gcaaatcccg atattatgca
     5341 ggcacagggc accgccattg gcgaagccat aaacctcgcc agggtatcat tcgacaaaaa
     5401 cagcaaaaac aaaaaagtca tcattgtaat ttctgatggc gaaaaccacg aagacgatgc
     5461 cattgaggca gcgcgtgcag ccgccaacga cgggctgctg acctatacca tagggatagg
     5521 atctgcttca ggggcaccca taccggtgag catgtcggga tttaacatag aatacaaaaa
     5581 agataactca ggtaataccg tagtgaccaa acttaacgaa accatgcttc agcaggtggc
     5641 ggctgcaggc aaaggaactt atgtaagagc taccaccaat gatgccggac tgaacaaggt
     5701 ctttgacgaa atcaacaagc tggaaaagaa agaatatgaa gcccggattt tttcagaata
     5761 ccgcaatacg tacatgtatt ttgtcattgc agccttgctg ctcctgattg ccgacctgat
     5821 tatactggaa agaaaaacgc gcctgacacg caacgtacac ctttttgaaa aaaaacatat
     5881 aatttcgtag tctgatttaa tatgaaaatg acagttttac atagtaaacc aggaataaaa
     5941 agttggtgtt ttcaacttac agtaatcata gtactgttgg gtattaccac tcatgtgcct
     6001 gctcaaacgg cggtgaaaga gctaagggca ggcaacaaac tttttgaaaa aaacgactac
     6061 aaatcagctg aaataagcta ccgcaaagcc ctcgaaaagc aaaacaatta ccagaaggcc
     6121 cttttcaacc tcggcgatgc catgtacaaa cagggaaaat acgaagaatc ggaaaagatt
     6181 tttcaaaacc ttaccgaatc agatattcct aaagaaattg ccgccaaagc ataccataat
     6241 ctcgggaaca gccagcttca gcaaaaaaaa taccaggaaa gcgtggaatc gtacaaacag
     6301 gcgctgcgcc aaaagcccaa tgataatgaa accaaataca acctggagta cgccaaaagg
     6361 atgatccaga aacaacagca acagcaacaa cagcagcaac agcaacaaaa ccaggaccag
     6421 aaacaacagc agcaacaaca gcaacagcag caacaacaaa ataaagatca gaagcaagac
     6481 aaaaatcagc aaaaccaaca aaaccagcaa aaacaacaaa tatcaaaaga agatgccgaa
     6541 cgtatgctga acgccttgca gaataaagaa aaaaacctca gggaaaaaat ggaaaaagaa
     6601 caacaaaacc agaattataa aacggagaag gattggtaga ggaagggatg gagtgattga
     6661 gttgaaaagg aaataagtta attgagttaa atggggaaat aagggagtga gggaatgagg
     6721 aattgagtag attaagttaa ttgaggaatt aagttaatta agttaattga ggaaatgaga
     6781 aaatgaggaa atgagggggt aaaggggatt gaggaaataa gagattaagt ggattgagag
     6841 attaagagaa ttagatccgg tatcggttgg gagaatagga aacctggagc gtgttataaa
     6901 ggtaatgcaa caaaaaaaat gaaaggaatc atcaccaaat acagacattg tgcgttaatc
     6961 attgtaacca tagttttttg taatgttccg ggcattgctt tggctcagaa atttacggca
     7021 tcagccagtt ctgcaagcgc cggattaaac aattcgatac ggataactta ttccctgtcg
     7081 gatgcccagg ccgaaagttt ccagccacca tcgttcagcg gttttaatgc cagcgggcca
     7141 tacaattcgt cgagcagcaa catttcattc attggcggga aatcggtggt gcagagcact
     7201 cagagctgga tatacagcct tacgcccacc aaagcaggaa agtttaccat cgaacctgcc
     7261 aaagttaagg taaacggaca atggatttcg tccaacagtc ttacgattga agtagctaat
     7321 accggcaccg cccagggcaa tcaaaatcaa aagcaacagc aacaaacaca gcaaaaccag
     7381 cagtcctcca ccgccgaagc ctctgccacc gacctttttg taaaagtatt taccgacaaa
     7441 accaatgtga tgcagggcga acaacttacc gttacctaca aaatatatac ccgcatcccc
     7501 gtgtcgcaat acgccataca aaaactgccg tctttttcgg gattctggtc gtacgacctg
     7561 atgaaaaaca aagaacaggc cgttcaatac aacgaaaccg tgaacggaca gaaatatacc
     7621 gtggccgaga ttcgcaaggt ggcgttgttt ccgcaaaagt caggaaaact aaccgtggac
     7681 cctctcgagg tggaagccct ggctcatatc ctcgtcaaga aaaaagccaa caaccccttt
     7741 ggtaatttct ttaatgatcc tttctttaac gacccctttt tccagaacgc ctttaccagc
     7801 ggctaccagg aagtaaaaaa gacactcaaa tccaacgccc tctccatcaa tgtttcggcg
     7861 cttcctgccg gacaacccga agatttttgc ggactggtgg gcaatctgaa catggaagcc
     7921 actctcgaca aaaacgaagt aaaagccaat gatgcagtta ccctgacagt gaaaatcagc
     7981 ggcaaaggaa acttaaacct catcgacaac ctaaacatcg aatttccgcc cgactttgaa
     8041 gtatacgacc cgcaaatcac cgaaaatatt tcctccggaa acgaaattac cgggtcaaaa
     8101 acatttacct acctgatcat tccgcgcaca cagggcaact tcaaactaaa cccgattaca
     8161 ctgagctatt acgataaaaa caaaaagtca tacgcaacac tgagctcagg cgaaatcaac
     8221 cttaaagtag gcaagggcga tggaagcggc ggtgcctaca taagcagcac cagcaaagaa
     8281 gacataaaat acattggcag cgacatcaag ttcattgaaa acaaggattt tgaagttttt
     8341 ccgggagatt atttcttttt tggctctgca tggtactggt ttctgctggt gttaccggta
     8401 ttattgttta ttgcctttgt aatcctgatg cgccgttata tcaaattgca gagtaacacg
     8461 gccctgctca aaaacaggcg tgccaccaaa gtcgccttaa aaaggttaaa acaggccaac
     8521 gccttcatga aatccaacga tcacaataat ttttaccagg aactctcgag ggccttatgg
     8581 ggatatgtct gcgataagtt ttcgataccc ttgtcggaat tgtcgctgga caccgctcgc
     8641 gaaactctta caaataaaaa tattgaagaa agcatacgca ccaaattcat tgaaatactc
     8701 gacgattgcg aatttgcccg ttttgccccg gcagaggcaa ccaaaggcat ggaaacttta
     8761 tacaacgaag cagtggaaat aatcagtaaa acggaaagag agttgaaata aaaaataatg
     8821 a
//
LOCUS       DAOSFP010000050         8758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6838_length_8758_cov_3.588992, whole genome shotgun sequence.
ACCESSION   DAOSFP010000050 DAOSFP010000000
VERSION     DAOSFP010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6838_length_8758_cov_3.588992"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..727
                     /locus_tag="PLI16_02520"
     CDS             176..727
                     /locus_tag="PLI16_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83463.1"
                     /translation="MKDCIIMRRLLSIFLISALFFNVFAYFLAFRIALKNINRHYHGR
                     ICNFSYGSAGDFIILDDALLTDENANGLFGNDDYIYQGRPYEFVDIAETRDITLPVTS
                     GEQDNEKWNTVFNQHLTQLDKKGMPVQQPTGRLIIPVFKVPVPEKLTLHIFQQGKQYQ
                     PFFYFYSVQTAVLNTVFVPPKMS"
     gene            814..3222
                     /locus_tag="PLI16_02525"
     CDS             814..3222
                     /locus_tag="PLI16_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83464.1"
                     /translation="MKKNIILLFFICSFCFQGFSQKLFVGDLKTGQAVENVALSSKKP
                     FAVAATDAKGETDITGFTGAERIEIRHLGYKTEYLSYAELSRLEFKLFIKPVGVSLDE
                     VVVSATKWSQQANDIPGKVTVIHPKEISMQNPQTAADMLAASGKVFIQKSQQGGGSPM
                     IRGFATNRLLYSVDGIRMNTAIFRAGNLQNVISLDPFAIEEAEVLFGPGSVIYGSDAI
                     GGVMSFQTLTPRLSTTGTTLFTGNAVSRYSSANNEITAHFHAGVGWKKWALLTSITTS
                     KFGDLKMGSYGPDEYLRPFYVQRQDSVDVVVTNDDPKVQRPSGYSQINMMQKIRFTPS
                     EKWDIQYGFHYSTTSDYSRYDRHIRYKKGLPRYGEWYYGPQKWMMNALNVTHNGNNKV
                     YDQMTVRMAWQSFEESRIDRDINKDDRHIRVENVEAFSGNIDFNKSLGDKHQFFYGVE
                     AVYDDVKSTGQDEDISTGTIVDGPSRYPRSNWSLYAAYLNYQFKISKKVLLQSGVRYN
                     HYMLNADFDTTFYPFPFTTAKINKGALTGSLGVVYRPTEKWVLNLNLSTGFRSPNVDD
                     LGKVFDSEQGSVVVPNPGLKAEYAYNAEIGVAKVFGDIVKIDVTGFYTYLNDAMVRRD
                     FTMNGSDSLMYDGAMSKVQAMQNAAYAQIIGVQAGFEVKLPVGFGLSSDFTYQKGTEE
                     LDDGSKSPSRHAPPWNGVSRLSYTYKKMSLQLYSVYSGGKKFQDLPEEEKGKPEIYAV
                     DGDGNPYSPGWYTLNFKGMYDLTDNFTISAGLENMTDQRYRPYSSGIVAPGINFVIAL
                     KAKF"
     gene            3265..5004
                     /locus_tag="PLI16_02530"
     CDS             3265..5004
                     /locus_tag="PLI16_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH83465.1"
                     /translation="MKKQTLVLLLCLGSLMLQAKPVERTTAISIARTFYYSQLNIADA
                     SKQAPAVISTGEYFTGNRLSYYLVNFSNKGWVLVSADDACFPVIGFSDEGNLDVTDLP
                     PALRWWLDGQSDEAVTATGKDAAYAAYISGAWEKLSKGSLEKSHPKTLVNPLLTSRWN
                     QDAGYNFHCPEYPSGPGGRCYAGCVATTMAQIMYYHKYPEHGTGSHYYSHVYFGTLGA
                     NFEETTYDWPAMTNTLNNSSKEAISTLISHCGLSVDMNYSPLGSSSSSTNVADALKDY
                     FGYSFRVKYYEKSDFENDQWHQMLIDNLELQLPVYYSGSGSSGGHAFVCDGVKDSCYF
                     HFNWGWSGYGNGYFYLSNLNSGNGDFSAAQAAVMYIAPYDYPYCMGEKQLNSAYKSFN
                     DGSNFSYYWNDTDCSWLIAPDSADHITLTFNSFSTEENHDILSIYKGDDETDSLIGQF
                     SGNTIPAPITVSGNRLYLRFVTDGNVQAQGWTATYSTIDFSGITEETGVAEIKPYPNP
                     ASDFVSIRIPDFGSRTLITFYNLTGEHFALPALDEGKNQLKIDVKDLPQGCYFVKVVS
                     EEKTHFGKVLIQR"
     gene            complement(5097..5744)
                     /locus_tag="PLI16_02535"
     CDS             complement(5097..5744)
                     /locus_tag="PLI16_02535"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH83466.1"
                     /translation="MIKQLLLFLCTGFLCLSSLMAQETKKAPAGLGFEAGMGYNMLTL
                     QGKSLAGNDTTVFYKRFWMQPCIRLHYDIKIKDFSGGRSTLKVKTFLGYYAFGGKAKM
                     SDIGEYDIVSLSSIEAGAGLSFDIIEMVQISPLIKGQYVFAASERYIRETPKPSTDIK
                     DQTNLFSYNAGLQLRFKYKHFAIAAEGWYGLADIHKDKNKTAKENNFRLLLGYEF"
     gene            complement(5826..7781)
                     /locus_tag="PLI16_02540"
     CDS             complement(5826..7781)
                     /locus_tag="PLI16_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83467.1"
                     /translation="MKKIFTLIILAAILFETDQISAQAPMLYYKFENNLSASTPNCGY
                     PLAGNALGTLTGVTFAGGGQFDTCLFGTGAYSSGINTGWNLNLGSKGWTISMWLSIPS
                     PTTNASYFFGDPAISFRCFQGGVAGQNNLFLRGTGITDVLVTGIGPAPTVVTFVYDSA
                     TSAIKAYKNGVLDTIVSQTPLNLGAGTGFKVGGYSTNMSMTGYMDEFRLYNRALSDAE
                     VAAMWNHDSECGDANFGLLLPTPGVNSNYVSIPYVPAMNDFNKITIEAWIKPGGFTTA
                     NTVLNKGGGSFDYQLGITSAGSPFFRVQSSIISSVGLTVNAGEWTHLAVTYDSSNVRF
                     YKNGILMSVVPCTTALGVSTNEMRIGRGNNDPGSGVIEELRLWSVVRTNAEINANKCR
                     KYPSEFSSSAGLKALWHFDGNLMDSVSGFNGIRQGISGYEEFIFPDPLMTCTCPSTSA
                     SMTAEACFRLTSPSGNYVWTSSGIYNDTITNLAGCDSIITLDLTINTVDTSVSQNGII
                     LTANATGVTYQWLDCDDAYAVIPGADGQSFDPPSNGNYAVEILDGSCKDTSSCHNIAT
                     FGIAGQDPELTLKVFPNPGNGDFTITSGIVADEIRITDILDREILKFKPEKKIVKVSI
                     GSHGKGFYMIRLNFKDQQKIARVIVNR"
     gene            8114..>8758
                     /locus_tag="PLI16_02545"
     CDS             8114..>8758
                     /locus_tag="PLI16_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOH83468.1"
                     /translation="MKSNFYKLSLYLAGFLLLLVSCKPLATQYEEDEITDAEYYEAVE
                     KTPVPQPDSAVRAMTWNIRFGIGRSEWFGDACSNITVYSQEDVYPHLDAVVAKINEIK
                     PDILLLQEVDINSKRTSYINELQYILDNTYFNYAAYVPEWCAQNIPSDGLGRMDMGLA
                     ILSPWPISDAQRVQLALRGDQSGIEVYFYLRSCMITASIQIPGFEELSVVNIHAS"
BASE COUNT         2455 a         1896 c         1902 g         2505 t
ORIGIN      
        1 ttcaaagtta atcaaaatta tcaaatgaaa aaattcttca aagcctaaat cgcagacctt
       61 gtcgaaccat atgaaatctt catcatgctc aatctccggc atgcttttag gggtgagaga
      121 ataatcataa tttttattgt tcatttgtca ggcttatgta tatttgctga aatttatgaa
      181 ggattgcatt attatgcgtc gccttttatc catatttctc atatcggcat tgtttttcaa
      241 tgtttttgcc tattttcttg cttttcgcat cgctttaaaa aatattaaca ggcattatca
      301 cggacgcatc tgcaatttca gttatggaag tgccggagat tttattattc tcgatgatgc
      361 actacttaca gatgagaatg ccaacgggct gttcggaaat gatgattata tttatcaggg
      421 aaggccgtat gagtttgttg acatcgcaga aacccgtgac atcaccctgc cggtaacttc
      481 cggtgaacag gacaatgaaa aatggaatac tgtatttaat caacacctga ctcagcttga
      541 caaaaaaggg atgcccgtgc agcagcccac aggcaggctc atcatacccg ttttcaaagt
      601 gcctgttcct gaaaaactaa cccttcacat ttttcagcaa ggaaaacaat accagccatt
      661 tttctatttc tattcggttc aaacagctgt tttaaataca gtttttgttc caccgaaaat
      721 gtcataattg caatcaggaa tctcaatcta ttggcctaaa gtattttatc agccaatgtt
      781 tactattttt atcacattaa ttatcatagt actatgaaaa aaaatataat cttattgttt
      841 tttatctgca gtttttgttt tcagggattt tcgcaaaaac tgttcgtcgg cgaccttaag
      901 acaggacaag ctgttgaaaa tgtcgccctg tcgagcaaaa aacccttcgc cgttgctgcc
      961 accgatgcta aaggagaaac agacatcacg ggatttaccg gggcagagcg tatagaaatc
     1021 aggcatttag gatataagac agagtatctg agttatgccg aactgagccg tttagagttc
     1081 aaattattca ttaaacccgt gggcgtttcg ctggatgaag tagtggtttc ggccaccaaa
     1141 tggagtcagc aagccaatga tattccggga aaagtaaccg ttatccaccc taaagaaatc
     1201 agtatgcaga atcctcaaac ggctgctgat atgctggctg cttccggaaa agtgttcata
     1261 caaaaaagtc agcaaggcgg tggaagtcct atgatcagag gatttgccac caaccgcctg
     1321 ttgtattcgg tggatggtat caggatgaat acggccatct tcagggcggg caaccttcaa
     1381 aatgttattt cgctggatcc ttttgccatc gaggaagccg aggtgctgtt cgggccgggt
     1441 tctgtaatat acggcagtga tgccattggc ggggtgatga gttttcaaac cctgacaccg
     1501 cgtttatcca ctacgggaac tactctcttt acaggcaatg cggtatcgcg ttattcctct
     1561 gccaataatg aaattaccgc acattttcat gccggcgtgg gttggaaaaa gtgggcgctg
     1621 ctgaccagta ttaccaccag caaattcggc gacctgaaaa tgggaagtta cggaccggat
     1681 gaataccttc gccccttcta cgtacaacgt caggacagtg tggatgtggt tgttacaaac
     1741 gacgacccaa aggtgcaacg gccttcagga tattcgcaga ttaacatgat gcagaaaatc
     1801 cgtttcactc cttctgaaaa atgggatatt caatatggct ttcattattc cacaacctcg
     1861 gattattcgc gctatgaccg ccacatccga tataaaaaag ggttgccacg ttacggagaa
     1921 tggtattacg gcccccagaa atggatgatg aacgcgctga atgtaaccca taatggcaac
     1981 aacaaggtgt atgaccagat gaccgtgcgc atggcctggc aatcttttga agagagccgt
     2041 atcgataggg acatcaacaa ggacgacagg catataaggg tggaaaatgt agaggctttc
     2101 tccggcaata ttgattttaa taaatccctg ggcgataagc accagttttt ctatggcgtg
     2161 gaagcggttt atgatgatgt taaatccaca ggacaggatg aagacatatc cacagggaca
     2221 attgttgatg gcccttcgcg ctatcccaga tcaaactggt cgttgtatgc ggcttatcta
     2281 aactatcagt ttaaaatttc caaaaaagtc ctgctacagt cgggtgtcag atataatcac
     2341 tatatgctca atgctgattt tgacaccacg ttttatccat ttccttttac cactgccaaa
     2401 atcaataagg gtgcgcttac cggcagtctg ggcgtagtat atcgcccaac ggaaaaatgg
     2461 gtgctgaatt tgaatctttc cacaggtttc cgttcgccca acgtggatga tctgggtaaa
     2521 gtatttgatt ccgaacaggg atcggtggtg gttcccaacc ccggtctgaa agcagaatat
     2581 gcctacaatg ccgagattgg tgttgcaaaa gtgtttggcg atattgtaaa gattgatgtt
     2641 accggttttt atacgtatct gaacgatgct atggtacgac gtgattttac gatgaacggc
     2701 tcggacagcc tgatgtatga tggtgccatg agcaaagtac aagccatgca gaatgccgct
     2761 tatgcgcaga taataggtgt gcaagccgga tttgaggtaa aactgccggt gggttttggg
     2821 ctttcgtctg attttactta tcagaaaggc accgaagaac ttgacgacgg cagcaaaagc
     2881 ccctcgcgcc atgcaccacc ctggaacggt gtgagcaggt tgtcctatac ctataaaaaa
     2941 atgtcgttac agttatactc agtatatagc ggcggaaaaa aattccagga ccttcctgaa
     3001 gaagaaaaag gcaagcctga aatttatgcc gtggatggtg atggcaatcc gtattcgccg
     3061 ggttggtaca cacttaactt caaagggatg tacgacctta cagacaactt cactatcagc
     3121 gcagggctcg aaaatatgac cgaccagcgg tatcggccat acagctccgg cattgttgct
     3181 ccgggaataa atttcgttat cgcactaaaa gccaagtttt aaatgaatta tttaatattt
     3241 ttgctgttta aatttcgaaa ttttatgaaa aaacagacac ttgtcctgtt attatgtctg
     3301 ggcagcctga tgcttcaggc aaagcctgtg gaacgcacca cagccatcag tatagcccgg
     3361 actttttatt atagtcaact aaacattgcc gatgcctcaa aacaggcacc ggcagtgatc
     3421 agtaccgggg aatattttac cgggaacaga ttgtcttatt acctggtgaa tttcagcaat
     3481 aaaggctggg tattggtatc tgccgatgat gcatgttttc ctgttatagg cttttccgac
     3541 gaaggcaacc ttgatgtgac cgacctccct cctgctttgc gctggtggct tgatggacaa
     3601 tccgacgaag ccgtaacggc aacaggcaaa gatgcagcat atgctgcgta tatcagcggc
     3661 gcctgggaaa aactcagcaa gggaagcctg gaaaagagtc atcccaagac actggtaaat
     3721 ccactattga ccagccgctg gaatcaggat gccggctata attttcattg tcctgaatac
     3781 ccttcgggtc ccggcggaag atgttatgca ggctgtgtgg ctacgaccat ggcccagatt
     3841 atgtattatc acaaataccc cgaacacggc accggcagcc attattacag tcatgtttat
     3901 tttggtacgt tgggagccaa cttcgaagaa acaacttatg actggccggc tatgacgaat
     3961 acgctcaaca atagcagcaa agaagccatt tcaaccctga tttcgcattg cggattgtcg
     4021 gtagatatga actactcgcc gctgggctca tcctccagtt ctactaatgt tgccgatgcc
     4081 ctgaaagact atttcggcta ttcctttcgt gtgaaatact atgaaaaaag cgactttgaa
     4141 aacgatcagt ggcatcagat gcttatcgac aacctggaac tgcagttacc tgtgtactat
     4201 agcggctcgg gcagcagtgg cggacatgcc tttgtgtgcg acggggtaaa agactcgtgc
     4261 tattttcatt ttaactgggg atggagcggc tatggcaacg gatattttta tctttcgaac
     4321 ctgaactccg gcaacggcga tttttcagcc gcacaggcag ccgtcatgta catcgcccct
     4381 tatgactatc cttattgcat gggcgaaaaa caattaaact ccgcgtacaa atcgtttaac
     4441 gacggcagca atttttcata ttattggaac gacacagact gcagttggtt gatcgccccc
     4501 gattctgccg accatatcac gctgacattc aattcgtttt ctactgaaga aaatcatgac
     4561 atcctgagta tatacaaagg tgatgatgaa accgattcgc ttattggaca attttcagga
     4621 aatactattc ctgctcctat aactgtttcg ggcaacagac tatatttgcg ctttgttacg
     4681 gatggaaacg tgcaggcaca gggctggaca gccacttatt ccacaatcga tttttcgggc
     4741 attactgaag aaaccggtgt cgcggaaata aagccctatc ctaatcctgc ttccgatttc
     4801 gtcagcatta gaattccaga ttttggttcc cggacattaa taacatttta taatctgacc
     4861 ggcgaacatt ttgctctgcc cgcattggat gaaggcaaaa atcaactaaa aattgatgta
     4921 aaagaccttc cgcaggggtg ctattttgta aaagttgtca gcgaggaaaa aacccatttt
     4981 ggaaaagtgc tgattcagcg ttaattattt tttttattaa taaaaaaggc cttttcgggg
     5041 cctttttttt tgcaactata cgaattgccg gtattttttt tcggggaagt ccgtttttaa
     5101 aactcatacc cgagaagtaa cctgaaattg ttttctttgg cggttttgtt tttatctttg
     5161 tgaatatcag ccaggccata ccagccttcg gcagcaatgg caaaatgttt gtatttaaag
     5221 cgcagttgca ggcctgcgtt ataagaaaac aaatttgtct gatccttaat gtctgtgctt
     5281 ggttttggcg tttccctgat gtaacgttca gaggctgcaa aaacatattg gccttttatt
     5341 aatgggctga tttgtaccat ttcaataata tcaaacgaca gccctgcccc tgcttcaatt
     5401 gaagaaagtg atactatatc gtattcgccg atatcactca ttttagcttt gccgccgaag
     5461 gcataatagc ccaaaaaagt ctttaccttc aaggtgctcc ggccgccgct gaaatctttt
     5521 atttttatgt cataatgcaa tctgatgcag ggttgcatcc agaaacgctt gtaaaatacc
     5581 gtggtatcat tgcctgctaa cgattttccc tgcaaggtta gcatgttgta tcccattccg
     5641 gcttcaaaac ccagcccggc aggtgctttt ttggtttcct gggccattag tgacgaaagg
     5701 cacaaaaatc ctgtacaaag aaacagcaat aactgtttta tcataatgat gaattttact
     5761 gcaaatttag tatttctgtg ccgtaagtca gaaattcgcc ctgatttttg tgaaagtgga
     5821 ttttatcagc ggttcacaat gacgcgggcg attttttgct gatctttaaa attcaacctg
     5881 atcatataaa aacctttacc atggcttcct atgctcactt tcacgatttt tttctccggc
     5941 ttaaatttta aaatttccct gtcaagaata tcggtgatgc gaatttcatc ggctacaatt
     6001 cccgaagtga ttgtaaaatc gccgttaccc gggttgggaa atacttttag tgtcagttcc
     6061 ggatcctgac cggcaattcc aaatgtagca atattatgac atgatgatgt gtctttgcat
     6121 gatccatcca gtatctccac tgcataatta ccgttggatg gagggtcgaa actttgcccg
     6181 tcagcgccgg gaatgacggc atatgcatcg tcgcaatcca gccattggta agtaacgcct
     6241 gtggcattag ccgtaaggat aataccattt tgtgaaacac tggtgtcaac ggtattgatg
     6301 gtcaggtcga gcgtaataat actgtcgcag ccggccaggt tggtgatggt atcattatat
     6361 ataccggaac ttgtccagac atagtttccg ctcggagatg tgagtcggaa acaagcttcc
     6421 gctgtcatgg aggcgcttgt tgagggacag gtgcacgtca tcagcggatc tggaaatatg
     6481 aattcttcat atcctgaaat tccctgcctt ataccattaa aaccactcac tgaatccatc
     6541 aggtttccat cgaagtgcca cagggctttc aaccctgcag aagaagaaaa ttccgacgga
     6601 tatttccggc atttgttagc gtttatttcg gcattagttc ggaccactga ccacaacctc
     6661 aactcctcga taactcctga tccggggtca ttgtttccac gtccgatgcg catttcattg
     6721 gttgatacac ccagtgctgt ggtgcagggc accaccgaca tcagtatgcc gtttttataa
     6781 aaccttacat ttgagctgtc gtaggtaacc gccaaatgtg tccattctcc ggcatttacg
     6841 gttaatccca ccgagctgat aatactggat tgcactctga aaaacggaga accggcagag
     6901 gtgataccca attgataatc gaagctgccc ccacccttat tcagaactgt attagctgtt
     6961 gtgaagcccc ccggtttaat ccatgcctca attgttattt tattgaaatc attcatggcc
     7021 ggaacatacg gaatactcac ataattggaa ttgactcccg gtgtgggcag taataagcca
     7081 aaattggcat cgccgcactc cgaatcatga ttccacatag cggccacttc ggcatcggac
     7141 aacgctctgt tatacagcct gaattcatcc atataacctg tcatagacat atttgtacta
     7201 tagccaccga ctttgaatcc ggtacccgct cctaagttca atggagtctg gctgacaatc
     7261 gtatctagta caccgttttt atatgctttg attgctgatg ttgcagaatc atacacaaag
     7321 gttaccactg ttggagccgg tcctatgccg gttacaagaa catctgttat tccggttcct
     7381 cttaaaaaca gattgttctg tcctgcaaca ccgccctgga aacaccggaa agaaatagcg
     7441 ggatctccaa agaaatacga tgcgtttgta gttggagaag gaatggataa ccacatgctt
     7501 atagtccagc cttttgatcc aagattcaga ttccagccgg tattaatccc gctggaatat
     7561 gcacccgtac caaacaaaca agtgtcaaac tgtcccccgc cggcaaacgt tactccggtt
     7621 aatgtcccta aagcattgcc tgcaagcgga tatccacagt ttggtgttga ggctgaaagg
     7681 ttgttctcaa atttataata cagcattggt gcctgagctg atatctggtc agtttcgaaa
     7741 agaatggctg ccagaataat taatgtaaaa atctttttca tgtttttaat ataaagttgt
     7801 ttcgatgtgt aaatatataa attttattga ataaaagcaa tatttttcta ttaattaaca
     7861 attagttggt gatactattt tggatgaaaa aggcaaattt catttcagtg aaccaaaact
     7921 aacaataccg gcaaaaataa tgaaggaaaa cggctgtctc aaaagccctt tgtggtaaat
     7981 aatatgtagt gttccgtata tcgctgatga taatgttaca acccagattc aaaaacttat
     8041 ttagtaaaga taaataattt gccagcatca tttttaaata attttgaaat atctttatcg
     8101 aaaattttaa actatgaaat ctaacttcta caaattaagt ttgtacttag ccggtttctt
     8161 gttattgttg gtttcatgca aacctttagc cacgcaatac gaagaagatg aaataacgga
     8221 tgcagaatat tatgaggccg tcgaaaaaac acctgttccg caacccgatt cagcggtcag
     8281 ggccatgacg tggaacatcc gtttcggcat tgggcgttcg gagtggtttg gcgatgcgtg
     8341 cagcaacatc accgtttatt cacaggagga cgtatatcca cacctcgatg ctgtagttgc
     8401 aaaaatcaac gagataaaac ctgatatttt gctgctgcag gaggtggaca tcaattcaaa
     8461 gagaacttcg tatataaatg aacttcagta cattctggat aatacttatt ttaattatgc
     8521 cgcttatgta cccgaatggt gtgcccaaaa tattcccagc gatggccttg ggcgaatgga
     8581 tatgggactg gcgatactct ctccctggcc aatttcggat gctcaacggg tgcaacttgc
     8641 cttgaggggt gaccagagtg gcatcgaggt ctatttttat cttcgttcct gcatgattac
     8701 cgccagtatt caaatacccg gcttcgagga attatcggtg gtaaatattc atgcttca
//
LOCUS       DAOSFP010000051         8743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6851_length_8743_cov_4.221685, whole genome shotgun sequence.
ACCESSION   DAOSFP010000051 DAOSFP010000000
VERSION     DAOSFP010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6851_length_8743_cov_4.221685"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLI16_02550"
     CDS             complement(<1..247)
                     /locus_tag="PLI16_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83469.1"
                     /translation="MKTRMIFAIAFCMIFSGSFAQLRTVKNEAYQRGEYLKYKAYYDA
                     LLTGEVIAGTCTFKVKDENKKIKDRDTYHLEVIGKTKG"
     gene            complement(321..3086)
                     /gene="leuS"
                     /locus_tag="PLI16_02555"
     CDS             complement(321..3086)
                     /gene="leuS"
                     /locus_tag="PLI16_02555"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOH83470.1"
                     /translation="MDYNFVEIEQKWQRSWSEQKTFKAETDHSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYARYKRLTGFNVLHPMGYDAYGLPAEQYAIQTGQHPAVTTEK
                     NIARYREQLDKIGFSFDWDREVRTCDPHYYKWTQWTFIRLFHSWYNKKTDKAEPVSIL
                     VKEFETNGNTNINAACSDVPAFTASQWNAMSEKEQQDLLMHYRLAYLADTMVNWCPEL
                     GTVLANDEVSEGFSIRGGHPVIRKTMKQWLLRISAYADRLLNGLDTIDWPESIKEIQR
                     NWIGRSEGAMIKFPIKGHDGLFMEIFTTRPDTIFGVTYMTFAPEHEYVNLITTPERKE
                     AVDTYLNETKNRSERERIADVKRITGEFTGAYAINPFTNEEVPIWVGDYVLAGYGTGA
                     VMAVPAHDSRDWAFARHFGLPLKEVIAGGDITKEAFESKEGTCVNSGFINGLKAREGA
                     KAVIKKAEEGGFGYGKINYRLRDAIFSRQRYWGEPFPVYYKEGCPYTLDESALPLELP
                     AVDAYLPTETGEPPLARAQNWKTKEGYPLETNTMPGFAGSSAYYLRYMDPHNDKEYFS
                     KEANAYWQNVDLYIGGAEHATGHLMYSRFWNKFLFDLGLVCKEEPFKKMINQGMIQGR
                     SSYVYRIKGTNKFVSYNLKGAYDTDAMLVDINMVNNDILDIVEFKAWRGDLADAEFIL
                     EDGKYVCGSEVEKMSKSKFNVVTPDDLVEKYGADTLRLYEMFLGPLEQHKPWDTKGIE
                     GVFRFIRKLWRLFHDSNNVFCVSDETPTHDELKVLHKTIKKVREDIERFSFNTAVSTF
                     MIGVNELTELKCNKRAVLSDLTVILSPYAPHIAEELWHLLGNNYSVSTAVFPEFNPEY
                     IAANTFTYAVSFNGKMRFTIELPVDMLQADIEKNALSHKDAEKWLGGKAPRKVIIVPG
                     KIINIVI"
     gene            3442..4323
                     /locus_tag="PLI16_02560"
     CDS             3442..4323
                     /locus_tag="PLI16_02560"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOH83471.1"
                     /translation="MGHKDERFTRRRLTSSRITTIVSISLVLFAIGLAGLLLLHAKKL
                     SDHVKENIGFTVYMNKNVKEAEILQLQKELDAMEYVRSTEYISEEKAAETFQKELGDD
                     FVSFIGYNPLHTSIEVHLKADYAEDFYFTQVENMLTKKPIVKEVSYQRSLVKTINDNV
                     RKITLVLLVFGLLLLLIAVTLINNTIRLAVYSKRFLIKTMQLVGATSGFIRKPFLSVS
                     IIHGLISSFIAIGMLIVVIYISRREIPEIVSLQSIDLFLILFAFVILVGFIISVVSTF
                     IAVRKYLKLNVDHLYLQ"
     gene            4349..4714
                     /locus_tag="PLI16_02565"
     CDS             4349..4714
                     /locus_tag="PLI16_02565"
                     /inference="COORDINATES: protein motif:HMM:NF022741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOH83472.1"
                     /translation="MAQKRKPNIVKKSAQEPAIKTEPVVISKPETVKTYAKSKAAESK
                     NHGFLFDKRNYLLMLAGIVFIIIGLIVMAGGGSEDPNVFNPDIFNTQRLTVAPILILL
                     GFVIEVFAIMLKPSEKADA"
     gene            4711..5514
                     /locus_tag="PLI16_02570"
     CDS             4711..5514
                     /locus_tag="PLI16_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOH83473.1"
                     /translation="MTYLEAIIIAIVEGITEFLPISSTGHMIITEKLLGVESSEFTKA
                     FIVNIQFGAILSVVVMYLKRFFQSFDFYLKLAIACIPLILAFLLEKVIDQMLEQVLIV
                     AIALFAGGVVLIFVDKWFKDQEKSNKVMTNKNAFFIGLIQIISVIPGVSRSAATIIGG
                     MTQGLNRKQAAEFSFFLAVPTMLAASGYKLLSNYEVLFFNADKTFNTNNISLLLVGNL
                     VAFVVAMAAIKFLISFLTKHGFKLFGYYRIVVGLLLIILLMAGVDLQIL"
     gene            5511..6218
                     /gene="truB"
                     /locus_tag="PLI16_02575"
     CDS             5511..6218
                     /gene="truB"
                     /locus_tag="PLI16_02575"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOH83474.1"
                     /translation="MSQFFNFPEGEVLFIDKPYKWTSFDVVGKIRFILKHQLGIKKIK
                     VGHSGTLDPLATGLLIICTGKATKRIEEFLNLDKVYSGTFMLGATTPSFDLETAINQT
                     FDVSGLTEEDILKTSEKFIGPQDQVPPVYSAIKVNGKRAYEMARSQQECVLEPKRVHI
                     KSFTITKIDLPLVDFRVACSKGTYIRALARDFGESLGCGAHLTGLCREFIGNISLQDA
                     LSIQQFELLVSQQKPSQ"
     gene            complement(6328..8385)
                     /locus_tag="PLI16_02580"
     CDS             complement(6328..8385)
                     /locus_tag="PLI16_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83475.1"
                     /translation="MKKILLLLCIPLALSFTQCNRGDKTKTKQQMGSITQTAIDSTVN
                     NLIAKYGDQQKERITRGVSQAAALWTKTDGTENEFKEFCLQHFAGDTTALNALFGRLQ
                     TNFEVLFGNFNRISLDLKKPLHIDGGEMIPVDDMFGAYDPFAHLNDDFFANKIAFITV
                     LNFPFYTLKEKIELGPKWSRTQWAYARLGDMYTSRVPAEAQQQFSDALTKADMYISEY
                     NIYMENLIDDKGNTYFPKDMKLISHWGLRDELKANYQGENGLAKQKMIYQVMLRIIMQ
                     EIPDSAINNPSVQWNPYTNKVFKDKKELTAKPEDNNRYGHMLSLFNAIQKIDEYNPQY
                     PTYIQRAFEQGMEMPQQEVEKLFIDFVSSPEVKQVAQRISKRLNRKLEPFDIWYDGFK
                     PRSSISEDELTAKVRAKYKNVADFQNDLSNILVKLGFAKDKAEYIASKISIDPARGSG
                     HAWGTMMKGDVSHLRARFPKEGWNYKGYNIATHEFGHNVEQTLSMYDVDYYSLAGVPN
                     TGFTEALAFIFQKRDLDLLGIKNDNPNKEYLQTLDDFWGCYEIMGVSLVDMQTWKWLY
                     EHPKATKAELKEAVIANAKDVWNKYYADVLGIKDCPLLAIYSHMIDNPLYLSNYPVGH
                     LVQFQVEEYMKGKNLADETIRMFTYGRTIPEVWMKNAVGAPISIQPMLKATKEALAKT
                     EMP"
     gene            complement(8467..>8743)
                     /locus_tag="PLI16_02585"
     CDS             complement(8467..>8743)
                     /locus_tag="PLI16_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOH83476.1"
                     /translation="PFCEGSGEIRASISLIDDIENRIQYLLKEQNERKIKLLVHPYLY
                     AYLTKGLYSRHLQWFFKYKQWIKLGYSNNFHFLEYHFFNSAGEEIKL"
BASE COUNT         2256 a         1823 c         1973 g         2691 t
ORIGIN      
        1 ctcctttggt tttcccaata acttccaggt ggtaggtgtc acggtcttta atttttttgt
       61 tttcatcttt caccttaaaa gtacaggttc ccgctataac ttccccggta agcaaggcat
      121 catagtaagc tttgtatttc agatactctc cccgttgata agcttcattt tttaccgtcc
      181 tcaactgtgc gaaacttcct gaaaatatca tgcaaaaagc aatcgcaaaa atcattcttg
      241 ttttcatggt gttttggttt taacactatg caggcaaatt ctgttccaaa aaaacaattg
      301 acaatgcaat attttgaaaa tcaaatcact atgttgataa tttttccggg aacgatgatg
      361 acttttcttg gggcttttcc acccagccat ttttcggcat ctttatggct aagggcattt
      421 ttttcgatat ccgcttgtaa catatccacc ggcagttcaa tggtaaagcg cattttgcca
      481 ttgaatgata cggcataggt aaaggtattt gcggcaatat attccggatt gaattcgggg
      541 aaaacagctg tgctcacact atagttgttg cctagcaaat gccacaactc ttcagcaatg
      601 tgcggggcgt aaggggagag gataacagtg agatcgctaa gtaccgcacg cttgttgcat
      661 ttcagctccg tgagctcgtt tacgccaatc ataaaggtgc tgacggctgt attgaaagaa
      721 aaacgttcga tatcttcgcg tacctttttg atggttttgt gcaacacttt cagctcgtca
      781 tgagttgggg tttcatccga aacacaaaac acgttgtttg agtcgtgaaa caacctccag
      841 agtttgcgga taaaccgaaa aactccttct atgcctttag tgtcccatgg tttgtgttgt
      901 tcgagcggac caagaaacat ttcataaagg cggagggtgt cggcgccgta tttttcaacc
      961 aggtcgtcag gtgttaccac gttgaatttg gatttggaca ttttttccac ttccgatcca
     1021 cacacatact ttccgtcttc aagaataaac tccgcatcgg ccaggtcgcc ccgccaggct
     1081 ttgaattcaa cgatatctaa aatatcatta tttaccatgt ttatatccac cagcatagcg
     1141 tcggtatcat aggcgccttt taggttgtac gaaacaaatt tgttggttcc ttttatccgg
     1201 taaacatagc tcgaacgtcc ctgtatcatg ccctggttaa tcattttttt aaatggttct
     1261 tctttgcaaa ccagccccag atcaaacaaa aatttgttcc agaatctgga atacatcagg
     1321 tgtccggtgg catgttccgc accgcctata tacaggtcaa cattttgcca gtaggcgttg
     1381 gcttctttcg aaaaatattc tttgtcgttg tgggggtcca tatagcgcag gtagtaggcg
     1441 ctgcttcctg caaagcccgg cattgtattg gtttcgagag gatagccttc tttggttttc
     1501 cagttttgcg ccctggccag cggaggttcg cctgtttcgg taggcaggta ggcatccacg
     1561 gcaggcagct ccagggggag ggccgattcg tcgagcgtgt atgggcagcc ctctttgtaa
     1621 tatacaggaa aaggttcgcc ccagtaacgc tgacggctga atatggcgtc cctcaggcgg
     1681 tagtttattt ttccataccc gaaaccgcct tcttcggctt tttttattac agcttttgct
     1741 ccttcccggg ccttcagtcc gttgataaat cctgagttta cacaggtccc ctctttagat
     1801 tcaaaggctt ctttggtgat gtcgccaccg gcaataactt ctttgagtgg tagtccgaaa
     1861 tgcctggcaa aagcccagtc gcggctgtcg tgcgccggaa cagccattac ggcgcctgtt
     1921 ccatacccag ccagtacata atcgcccacc catattggca cttcttcatt ggtaaaaggg
     1981 ttgatggcat atgctcccgt aaattcgccg gtgattcttt tcacatcggc aatacgttcg
     2041 cgttcgctgc ggtttttggt ttcgttcagg tacgtgtcaa cggcttcttt gcgttcgggc
     2101 gtggtaatga ggttcacgta ttcgtgttcg ggggcaaagg tcatgtaggt aacaccaaaa
     2161 atagtgtcgg gacgggtagt gaaaatctcc ataaacagtc cgtcgtggcc tttgatggga
     2221 aattttatca tggccccttc cgaacggcca atccagttgc gttgtatttc tttaattgac
     2281 tcgggccagt cgatagtatc cagcccgttc aacaacctgt cggcataggc tgatatgcgc
     2341 agcagccatt gtttcatggt ctttctgatt accggatgcc ctccgcgtat tgaaaaaccc
     2401 tcacttacct cgtcgttggc aagcaccgtg cctaattccg gacaccagtt taccatggtg
     2461 tcggcaagat aagccaggcg gtaatgcatc agcaaatcct gttgctcttt ttcgctcatg
     2521 gcgttccatt gcgatgccgt aaaggcagga acatcgctgc aggccgcatt gatattggta
     2581 tttccgtttg tttcaaattc ttttaccaaa atggaaacgg gctcggcctt gtcggttttt
     2641 ttgttgtacc acgaatgaaa aagccggata aaggtccact gtgtccattt gtaatagtgc
     2701 gggtcgcagg tgcggacctc ccggtcccag tcgaatgaaa agcctatctt gtcaagttgt
     2761 tcgcggtaac gtgcaatatt tttttcggtg gtaacggccg gatgttggcc ggtttgtatg
     2821 gcatattgtt cagcaggaag tccgtaagcg tcataaccca tggggtgcag cacattaaat
     2881 cctgtaagcc ttttgtaacg ggcataaatg tctgaggcaa tataccccag gggatgtcca
     2941 acatgcaggc cggcccctga agggtagggg aacatgtcga gcacataata ttttggtttc
     3001 gaatgatctg tttcagcttt gaaagtcttt tgctcactcc aggagcgttg ccacttttgc
     3061 tctatttcca caaaattgta gtccatatct ctaaaggaaa aattaataaa taagttgcga
     3121 agttatgaaa ttctgtcgag aggataatga agcgcataga aaatgaattt tttgcggtaa
     3181 aacataaaac cggattatgc agcaataccc gcattctttt taccttacgt gccacttatt
     3241 ataaaatatt tttttattca atcgtattat aaccgcaaac actttactaa tcatatctga
     3301 attaagcagg ttaagccatc aaaaccgtac gcagtcaaca gaaaaccggg tgttactttt
     3361 atacaaagaa tgatgatatt tttacagggg aataataccg tattattaat tttactaatt
     3421 tagtccccca aataggacaa tatggggcac aaggacgaaa ggtttacacg gcgaaggctg
     3481 accagctcaa gaatcaccac catagtgagt atatcgctgg tattgtttgc cattgggctg
     3541 gcggggctgc ttttgctgca cgccaaaaaa ctttccgacc atgtaaaaga aaatattggc
     3601 tttaccgtat atatgaataa aaatgtgaag gaagccgaaa tcctgcagtt gcaaaaagag
     3661 ctggatgcta tggaatatgt gcgttctacg gagtatatca gcgaggaaaa agctgcagag
     3721 acatttcaga aagaactggg tgacgatttt gtaagtttta tcggatacaa tccgctgcac
     3781 acctcgatag aagtacatct gaaagctgat tatgccgaag atttttattt tacacaggtt
     3841 gaaaatatgc tgaccaaaaa acctattgtc aaagaggttt cttatcagcg atcgctggta
     3901 aaaaccatta acgacaatgt caggaaaatt accctggttt tattggtttt tggcctgttg
     3961 ttactgctta ttgccgttac gctaattaat aacaccatca gactggcagt gtattccaag
     4021 cgttttctta tcaaaaccat gcaacttgtc ggcgccactt cgggctttat ccgtaagcct
     4081 tttctatcgg tcagcattat tcacgggctg atatcttctt ttattgctat cggtatgctg
     4141 attgtggtga tttatatttc acgcagggag atccccgaaa tagtcagttt gcagagcatc
     4201 gaccttttcc ttattttgtt tgcattcgtt atcctggtgg gatttattat ttcggtagtt
     4261 tccactttta tcgcagtaag gaaatatttg aaacttaacg tagatcattt atatcttcaa
     4321 taattttttc agaaaaaaca tttttaaaat ggcacaaaaa agaaaaccca acatagtcaa
     4381 aaaatctgca caggaaccgg caatcaagac agaacctgta gtgatttcca aacctgaaac
     4441 cgtgaaaacc tatgccaaat caaaagcagc agaatccaaa aaccatggtt ttttgttcga
     4501 caagagaaat tacctgttga tgctggcagg gatagttttt attattatcg gcctgatagt
     4561 gatggccggc ggcggctcgg aagatcccaa cgtgttcaat cccgatattt ttaacacaca
     4621 acgacttacg gtagccccca tacttatcct cctgggtttt gtgattgagg tgtttgccat
     4681 catgctcaaa ccaagcgaaa aggcggatgc atgacctatc ttgaagcaat tattattgcc
     4741 atcgtagagg gcatcactga gtttttgccc atttcttcta ccgggcatat gattataacc
     4801 gagaaactcc tgggtgtcga aagttcggag tttaccaaag cctttattgt caacatacag
     4861 tttggagcaa tattgtcggt ggtggtcatg tacctgaaac ggttttttca gtcattcgat
     4921 ttttacctta agctggccat cgcctgtatt ccactgatat tggctttttt actcgaaaaa
     4981 gtcatcgacc agatgctcga acaggtgctg atagttgcta tagcgctttt tgccggcggt
     5041 gttgtattga tttttgttga caaatggttt aaagaccagg aaaagtcaaa caaagtaatg
     5101 actaataaaa acgcattttt tatcgggctg atacagatta tttcggtaat acccggcgtc
     5161 agccggtcgg cggccaccat catcgggggc atgacacagg gcctgaaccg caaacaggct
     5221 gccgaattta gcttttttct ggcggtgccc accatgctgg cggcctccgg atataagttg
     5281 ttgtcaaatt acgaagtgct tttttttaat gccgacaaga cttttaacac taacaatatc
     5341 tctctgctgt tggtgggcaa cctggttgct ttcgtggtgg ccatggcggc cataaagttt
     5401 ctgatttctt ttttgacaaa acacggattt aagcttttcg gatattacag aattgttgtg
     5461 ggcttgctgc tgataatttt gttgatggcc ggtgttgacc tgcaaatact atgagccagt
     5521 tttttaattt tccggaaggc gaggtgttat ttatcgacaa gccttataag tggacatctt
     5581 ttgacgtggt ggggaagata aggtttattc ttaaacacca actgggaatt aaaaagatta
     5641 aggtggggca cagcggaact cttgatccgt tggctacggg cctgctgatt atttgcacag
     5701 gtaaggccac caaaaggatt gaggagtttc ttaatctgga taaggtttat tcaggtactt
     5761 ttatgctggg tgctaccacc ccgtcttttg atctcgaaac cgcgatcaac caaacatttg
     5821 atgtttcagg cctaacagag gaagatattt tgaaaacttc tgaaaaattc atcggaccac
     5881 aagatcaggt accgccggtt tattcggcta taaaggtcaa tggcaaacga gcttacgaga
     5941 tggctcgaag tcaacaggaa tgcgtccttg agcccaaacg ggtacatatt aaaagtttta
     6001 cgattacaaa aattgatttg cccctggtgg attttagggt tgcatgcagc aagggcacct
     6061 atattcgtgc actggcacgg gattttggcg aatcgctggg gtgcggggca caccttaccg
     6121 gtctttgccg cgaattcatc gggaatattt ccctgcagga tgccttaagt atccagcagt
     6181 ttgagctttt ggtttcgcaa caaaaacctt ctcaatagtt ttttagtttc tatttataaa
     6241 cccttaggga gaattgcatt tttaaaaaaa atggcttgca gtaattacaa gccatttttt
     6301 gtgagagggt attgattatt attattttca gggcatttcg gttttagcca gggcctcttt
     6361 ggtagccttt aacattggtt gtattgaaat cggtgcgccc acggcatttt tcatccatac
     6421 ttccgggatg gtgcggccgt aagtgaacat ccgtatcgtt tcatctgcca ggtttttacc
     6481 tttcatgtat tcttccacct gaaactgtac gaggtgtcca acaggataat ttgacagata
     6541 caagggattg tctatcatgt gtgaataaat agccagcaaa gggcagtctt ttattcctaa
     6601 aacatcggca taatatttat tccatacgtc tttggcgttg gctattacag cttctttaag
     6661 ttcggccttg gtggctttgg gatgctcgta cagccatttc catgtttgca tatctaccag
     6721 cgacacaccc ataatttcgt aacaacccca gaaatcatcc agtgtctgaa gatattcttt
     6781 attcgggtta tcgtttttga tgcctagcaa gtcgagatcg cgtttctgaa agataaaggc
     6841 caaagcttca gtaaatcctg tgttaggaac acctgccaga gaataataat caacatcata
     6901 catggacaaa gtctgttcta cattgtgtcc aaattcatga gtggcaatgt tgtatccttt
     6961 gtagttccat ccttctttcg ggaaccgggc tctgaggtgc gatacatcgc ctttcatcat
     7021 ggttccccag gcatgtcctg agccgcgtgc cgggtcaata gaaattttgg aggcgatata
     7081 ttcggcttta tcttttgcaa aacctagttt aacaaggatg ttggaaaggt cgttctgaaa
     7141 atcggcaaca tttttatatt tggctctgac tttagccgtc agttcgtctt ccgatatgga
     7201 gcttctgggc ttgaatccgt cgtaccagat atcaaaaggt tccagcttgc ggttgaggcg
     7261 tttgctgata cgctgggcaa cctgttttac ttcgggcgaa gaaacaaagt cgataaacaa
     7321 tttttccact tcctgctggg gcatctccat tccttgttca aaagcacgtt gaatgtatgt
     7381 agggtattgc gggttgtatt cgtcaatttt ttgaatagcg ttgaacaggc tgagcatgtg
     7441 tccgtagcgg ttgttgtctt cgggcttggc cgtcagttct tttttgtctt taaaaacctt
     7501 gttggtataa ggattccatt gcacggacgg attgttgatg gcgctatcgg ggatttcctg
     7561 cataatgata cggagcatga cctgataaat cattttttgt ttcgcaaggc cgttttcgcc
     7621 ctgataattt gctttcagtt catcgcgcaa accccagtgg ctgataagtt tcatatcttt
     7681 gggaaaatag gtgtttcctt tatcatcgat aaggttttcc atataaatgt tgtattccga
     7741 aatgtacatg tcggctttgg taagcgcgtc ggaaaactgt tgttgtgcct cggcaggaac
     7801 gcgagaggtg tacatatcgc ccaggcgtgc atatgcccat tgtgtcctgc tccatttcgg
     7861 accgagttcg attttttctt ttaacgtata aaaaggaaaa ttcaaaacag taataaatgc
     7921 gatcttgttg gcgaaaaagt catcgttgag atgagcaaaa ggatcataag ccccgaacat
     7981 atcatccacg ggaatcattt cgccaccatc gatgtggagg ggctttttga ggtcgagact
     8041 gattctgttg aaatttccga acagtacttc aaagttggtc tgtaagcgtc cgaacagtgc
     8101 gttgagcgct gtggtgtcac cggcaaaatg ctgcaaacaa aattctttaa attcattttc
     8161 ggtgccatcg gtttttgtcc acagggctgc tgcctggcta accccgcggg taatgcgttc
     8221 tttttgttgg tcgccgtatt tggcaataag gttgttgacc gttgaatcaa tagcggtttg
     8281 cgtgatggat cccatttgtt gtttggtttt tgttttgtcg cccctgttgc attgtgtaaa
     8341 cgacagtgcc agaggaatac ataataaaag aaggattttt ttcatattac tttcaggttt
     8401 aggttaaaaa taaacgcagg cttttgaaaa ccaaaagtat gaaaaaaaag aaaataatcg
     8461 taataatcag agttttattt cttcaccagc gctgttaaag aaatggtatt caagaaaatg
     8521 gaaattgttg gaatagccaa gttttatcca ttgtttgtac ttgaaaaacc attgcaggtg
     8581 ccgtgaatag agtcccttgg tgaggtaggc gtaaagatag ggatgaacca gaagttttat
     8641 ttttctttcg ttttgttctt tcaaaagata ttggatacgg ttttcgatat cgtcgatgag
     8701 ggaaatgctg gccctgattt cgcctgatcc ttcacagaac gga
//
LOCUS       DAOSFP010000052         8605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_6993_length_8605_cov_4.122339, whole genome shotgun sequence.
ACCESSION   DAOSFP010000052 DAOSFP010000000
VERSION     DAOSFP010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6993_length_8605_cov_4.122339"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1099)
                     /locus_tag="PLI16_02590"
     CDS             complement(137..1099)
                     /locus_tag="PLI16_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH83477.1"
                     /translation="MKKILIVGAVGQIGSELTMELRRIYGNDNVIASTRKTKPSEIVA
                     DSGPFEYFDILNKQAMAEVCKKYQVDCIINMAAILSAVGEQNPLMAWDINMNGLINVL
                     ELAREMKMEKVLCPSSIAVFGPGTPLDCAPQETVLKPSTMYGITKVAGELICDYYVKK
                     YGMDVRGLRYPGIISNETLPGGGTTDYAVAIYYDAIKYGKYTCFVKEDTRLPMMYMPD
                     CLKATVDLLQADFAKLKHHSDFNVGAMSFSVKEMAENIKKYLPDFTIEYKPDFRQAIA
                     DSWPDAVDDKAAREEWGWKPSYDLDAMTQDMLKVIGEKHKKGLI"
     gene            1279..2919
                     /locus_tag="PLI16_02595"
     CDS             1279..2919
                     /locus_tag="PLI16_02595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOH83478.1"
                     /translation="MKKFIFLLIIGTLSPAMIYCQEDNTIKLNPPLNDVIIYLTGAQL
                     RYKVNVNLVKGRNVVVFENFAPGLNPQSIKIRAEENATVLSISNKVTSSPVENEIQKH
                     TRINDSIKLITKKISIINDEKNALTVQKEMLLKNQSLGGQSTGVNVAELQKAADFYQL
                     RIFDINKRLSDLATETDTQTALLSGLQRKADEINAKLNISRSEVSLLINANNTQATSL
                     DVSYVVNDAGWMPYYDLKCDDISQPITLNYRAKAYNNTGIDWNDVSLILSTTDPSLNI
                     SVPELKTWYLTNYVSQVNYGKSGWYNQAVTDNLENKETQKAEGKTQQQSVKTKQIEVP
                     ELSFDFNIKNYYTLPSDAKPYIVDIEEHSVKAGYRYVCVPVVEKSAFLMACINDWEEL
                     NLVEGYANVYLNGTYVGQSYINPNEIADTLQISLGRDSRIQVDRVKLKDFSSRVLIGT
                     KRKATYVYELSIKNNRTSPVNIEIQDQIPVSNSQEIEITVDEVSAAEHNPLTGFLKWN
                     YTIDTGKVLSLKVGYTVKYPKSYSVPMKRMRALNCPSF"
     gene            2940..3578
                     /locus_tag="PLI16_02600"
     CDS             2940..3578
                     /locus_tag="PLI16_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH83479.1"
                     /translation="MLTVKTFQFNDFGVNTYVLANGNKQCIVIDPGCYTSAEQKTFDD
                     FIETSQYHIEKIVITHCHIDHILGVTHLEDKFGVGAWIHPAGKEFLRASVGYASVFGF
                     ELDRVISPAGFVNEGEPIILGNDKIDVLYTPGHAEGSVCYLIHGQKTVFSGDVLFQGS
                     IGRSDLPTGDYDLLISSIKNKLLTLDDDYTVFPGHMAKTTIGNERKNNPYLL"
     gene            3575..4768
                     /locus_tag="PLI16_02605"
     CDS             3575..4768
                     /locus_tag="PLI16_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83480.1"
                     /translation="MMTMTRCRNIFWAIGVLLLLTVPAKGQSFFRGVLLSRQDSTVIP
                     FAIVKLAETGSYVETTIKGEFNFEISSGKTPVHLEISTFGLRETIIHQPTFKGTEKIY
                     VDQMPLVLNLVEVEGLNAEEIVKKAVLQIPANYTDSSFAAFSFYRQYEKINGKFKNLI
                     EVQAVVSFIINTVKNYKSADYGFAIEHMRKSNHTYVIDDLQYDDYDFSALLRQNPVYH
                     LAESSLNPNAFSFYEFSFDTLAEHTDDYTINYKCAGFSSEMHGVSNIREVGWQGESRE
                     EGVLIIDRKTFAFKKIERKAYRNPWYHYPKNNNYLLPSKRYYEEFVDGKLTVEYYQEK
                     DKWFPASLCYSYTNEFYKSATANKEFTITDVFEWHCDSVSHFIPSSLADIFYKDSPLP
                     LQKYT"
     gene            complement(4784..6334)
                     /locus_tag="PLI16_02610"
     CDS             complement(4784..6334)
                     /locus_tag="PLI16_02610"
                     /inference="COORDINATES: protein motif:HMM:NF033578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOH83481.1"
                     /translation="MAKACKNRASKNAYISQNQMTLEGFETPFSQKLHADNRWVKLSR
                     QIPWDLLVTTYQAQLNNSQRGAEGINPRVAIGAMILKHICNMSDRETVLQIQENMYMQ
                     YFIGYSSFSNEEPFDPSLFVEFRKRMGIEQINAINEKILGLSKKPEDNFPEDEAGSGD
                     GGDTTNEENTSIKQIAKDASLKEHRGKLITDATACPQDIAYPTDLNLLNDAREKSEQL
                     IDKLYLPQLHESKPRTDRKEARKLYLHTAQKKNKSKKEIRKAIKKQLSYLRKNLKSIN
                     LLLDVYPVIPLQQKDYKYLLVINTVYEQQQKMFSEHVHSIEDRIVNIHQPHVRPIVRG
                     KSNAYVEFGAKINVSLMNGFAFLDDLSWNAFNEGTRLMASVEKYRERFGYYPADVLAD
                     KIYCNRTNRAALKLLGITLHAKPLGRPKAVEDHISPGERNPIEGKFGQAKTAYGMNRI
                     KARLTQTSESWIATTVLVLNLVKLIGLGCYNYVIPLIFSAQRIVAHWVDFIRECTWLQ
                     TNNEYSAL"
     gene            complement(6573..8495)
                     /locus_tag="PLI16_02615"
     CDS             complement(6573..8495)
                     /locus_tag="PLI16_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOH83482.1"
                     /translation="MSESIKHECGIAFIRLLQPLEYYRLKYNTPLYGLNKLMLMMEKQ
                     HNRGQDGAGIACIKLDLEPGMRFINRSRSNSSQPITDIFSPAYTALKELRKTKPSLYS
                     DVNWLKHNADFVGELYLGHLRYGTFGKNSIETCHPFIRENSWETKNIVLAGNFNLTNV
                     DELFNKLISLGQHPVETTDTVTILEKIGHFLDEENEDLYQSYKKQGVSKKDVSALIAA
                     DLDVQRLLTRAARDWDGGFVLAGMIGHGDAFVMRDPAGIRPAYYYADDEVVVVASERP
                     AIMTTFNVAIEKVNEILPGNALIVKKDGSYRLSQFVQPAKKMSCSFERIYFSRGTDKD
                     IYLERKKLGSFLVPSILKAIDYDLENTVFSYIPNTAAVAYYGMMGALEKFCDGVKKDR
                     IMQAGNDITAKELEKILAFHPRFEKVAVKDMKLRTFITQDKDRDDLVAHVYDVSYGII
                     RDYADTIVVIDDSIVRGTTLRESIIRMLDRLNPKRIIIASSAPQIRYPDCYGIDMAKL
                     NDFIAFRAAIALLHETKQDDVINRVYKKCKAQEHLPKEKIVNYVKEIYEPFTPERIAE
                     KITQLLVPEDIRARVQIIFQSVSDLHAACPGNTGDWYFSGDYPTPGGNKVVNRSFINY
                     IEKRNERGYQAKMQGA"
BASE COUNT         2448 a         1883 c         1747 g         2527 t
ORIGIN      
        1 atctcggcac cttgcggact ttcaataata cgttcgtgtt taaaaagttt ttgttcttca
       61 agtttctgga gttcctctgc cagaaaggct ttcatttttg tttgcatagt taaatctcct
      121 tttatgattt tattttttat attaatcctt ttttatgttt ttcaccaatc actttaagca
      181 tatcctgagt catggcatca aggtcgtagc ttggtttcca tccccattct tcgcgtgcag
      241 ccttatcatc cacagcatcg ggccaactgt cggctatagc ctggcggaaa tccggtttgt
      301 actcgatagt gaaatcaggc aaatattttt taatattttc agccatttcc ttcaccgaaa
      361 aactcatggc gcccacgttg aaatcgctgt ggtgtttcag ttttgcaaaa tcggcctgca
      421 ataaatccac ggtagccttg aggcagtcgg gcatatacat catcggaaga cgggtatctt
      481 cttttacaaa gcaggtatat ttgccatatt taatggcatc gtaatagatt gccacagcat
      541 agtcggtggt gccgccgccg ggcagggttt cgttgctgat aatgccggga taacgcagcc
      601 cgcgaacatc cattccatat ttttttacat aataatcgca aatcaattcg ccggcaactt
      661 tagttattcc atacatagtt gatggtttca gcacggtttc ctgaggagca caatccagtg
      721 gcgttcccgg gccaaacaca gcgatagaac ttggacacag cactttttcc atcttcattt
      781 cgcgagccag ttcgagcaca ttgattaagc cgttcatgtt gatatcccag gccataagcg
      841 gattttgttc gcccacagcg gaaagaattg cagccatgtt aataatgcag tccacctgat
      901 actttttgca cacctcggcc attgcctgct tattcaggat gtcaaaatat tcaaaaggcc
      961 ctgagtcagc aacgatttca gaaggctttg tcttccgggt actggcaatt acattgtcat
     1021 tgccgtaaat tctgcggagt tccatggtaa gttcagaacc tatctgacca actgctccga
     1081 caataaggat ttttttcata tagattatta ggtttttaaa taaatgtttt ttttatttgt
     1141 cgcgcaaatt tagctttttt agccatactt tgtaataaac cactaaactt ttttgaataa
     1201 tattttttgc acaaaaaaac tactacattt tctaatttaa acctcgttaa atgcttattt
     1261 ttgaaaaaaa acaaatccat gaaaaaattt attttccttt taataatcgg caccttatct
     1321 cctgcaatga tttattgcca ggaagataac accatcaaac tgaatcctcc cctgaatgat
     1381 gtgatcattt acttaaccgg cgcccagttg cgatataagg tcaatgtaaa cctggttaaa
     1441 ggaagaaatg tagtggtttt tgaaaatttt gcccccggac tgaacccaca atcaatcaaa
     1501 atcagggcgg aagaaaatgc caccgtactt tccatctcga acaaagttac ttcctcgccg
     1561 gtagaaaacg aaatacagaa acatacgcgg ataaatgact ccataaaact aatcaccaaa
     1621 aaaatttcca tcatcaatga tgaaaaaaac gcattgacgg ttcaaaaaga gatgttgttg
     1681 aagaaccagt cgctgggagg gcaatcaacg ggtgtgaatg tcgcagagct tcagaaagca
     1741 gccgattttt accagttaag aatctttgat atcaataagc gtttatctga cctagccaca
     1801 gaaaccgata cacaaacagc attgctttcc gggttgcaac gtaaggctga tgaaatcaat
     1861 gccaaactaa atatttcacg gtcggaggtg tcgctgctta taaatgcaaa taatacgcaa
     1921 gctacctccc tggatgtttc atatgtagta aacgatgccg gttggatgcc ctattacgac
     1981 ctcaaatgcg atgatatcag ccagcctatc acgctgaact accgggccaa ggcttataac
     2041 aacactggga ttgactggaa tgatgtttcg ctgattctat ctacgacaga cccttctctt
     2101 aatatatccg tacctgaatt aaagacatgg tatctgacaa attacgtaag ccaggtcaac
     2161 tatggcaaat caggatggta caaccaggct gttactgata atctggaaaa taaagagacc
     2221 caaaaagcgg aagggaaaac acaacagcaa tcggtgaaaa caaagcaaat tgaggtcccg
     2281 gaattaagtt ttgattttaa catcaaaaat tattatacac ttccttctga tgcaaagccc
     2341 tatatcgttg acattgaaga acactctgtc aaggcgggct accggtatgt gtgtgttcct
     2401 gttgttgaaa aaagcgcttt tcttatggcg tgtatcaacg actgggaaga actaaatctg
     2461 gtagaaggtt atgcaaatgt ttatctcaat gggacctatg taggccaatc atacatcaat
     2521 cccaacgaaa ttgccgatac actgcaaata tcccttggcc gcgacagccg gatacaggtt
     2581 gaccgcgtaa agcttaaaga tttcagcagc agggtattaa taggcactaa acgaaaagcc
     2641 acctatgtgt atgaactttc aataaaaaac aaccgtacat cccctgtaaa tattgaaata
     2701 caggatcaga tacccgtaag taacagccag gaaatagaaa ttacagtcga tgaggtttcg
     2761 gcagcagaac acaatccgct tactgggttc cttaaatgga actatactat tgacacgggt
     2821 aaagtcctgt cgctaaaagt tgggtacacc gtaaagtatc ccaaaagcta ttccgtgccc
     2881 atgaagagaa tgagggcgct gaattgcccg agcttctaaa gatataaacc aatttaatta
     2941 tgcttaccgt aaagactttt caatttaacg attttggcgt gaacacctat gtcctggcca
     3001 atggaaacaa gcaatgcatc gtcattgacc cgggttgcta taccagcgcc gagcaaaaaa
     3061 cttttgatga ttttatcgaa acatcgcagt accatatcga aaaaattgta attacccact
     3121 gccatatcga tcacatcctg ggagtaacac atctggaaga taaatttggt gtaggggcat
     3181 ggattcaccc tgccggaaaa gaatttctga gagcttccgt aggatatgca tcggttttcg
     3241 ggttcgagct cgaccgggtg attagccctg caggctttgt taatgaagga gaaccaatta
     3301 tcctgggaaa cgataaaatt gatgtcctgt acacccccgg acatgccgaa ggcagcgttt
     3361 gctatttgat ccatgggcaa aaaacagtat tttcaggcga tgtattattt cagggaagca
     3421 ttggccgctc cgaccttcca acaggcgatt atgacctgct gatcagcagt ataaaaaata
     3481 aattattgac actggacgat gattacacag tatttccggg gcacatggca aagaccacta
     3541 tcggaaatga gaggaagaac aatccctact tgttatgatg actatgacca gatgcaggaa
     3601 catattctgg gcaataggag tcttgttgtt gctgacagtt ccagcaaaag gacaaagctt
     3661 tttcagaggc gtactgctca gccggcagga ctccactgtt ataccctttg ccatagtgaa
     3721 actggctgaa acaggtagct atgtggaaac taccatcaaa ggcgaattta atttcgaaat
     3781 ttcttcgggc aaaacgccgg tgcatttgga aatctccact tttgggctgc gcgaaaccat
     3841 tatccatcaa cctactttta agggaacaga aaaaatttat gttgatcaaa tgcctcttgt
     3901 acttaatctt gttgaggtag agggacttaa cgcggaagag atcgtaaaaa aagctgtttt
     3961 acaaatcccc gcaaactaca ccgattcaag ttttgcagcc ttttcgtttt accggcaata
     4021 cgaaaaaatc aatggtaaat ttaaaaacct gattgaagtt caggctgttg tctcgtttat
     4081 tatcaacacc gtaaaaaact ataaatcggc ggattatggc tttgccattg aacacatgag
     4141 aaaaagcaat catacctatg tcattgatga cctgcaatat gatgattatg atttttccgc
     4201 tttattaaga cagaacccgg tatatcatct ggctgaaagt tcattgaacc ccaatgcatt
     4261 cagcttttac gaattcagtt ttgacacgct cgctgaacat acagatgatt acaccatcaa
     4321 ctataaatgc gccggcttct ccagtgaaat gcacggagtg agtaacatca gagaagttgg
     4381 atggcagggc gaaagccgcg aagaaggagt tttaattatt gacaggaaaa cttttgcttt
     4441 taaaaaaatc gaaagaaaag catacagaaa cccgtggtat cattatccaa aaaacaataa
     4501 ttatttactg ccgtccaaac gctattatga agaatttgtg gatgggaagc tgacagtgga
     4561 gtattatcag gaaaaagaca agtggttccc ggcaagttta tgctacagct ataccaatga
     4621 attttataaa tcagcaacag caaataagga gtttaccata acagatgttt ttgagtggca
     4681 ctgcgacagc gtttcgcatt ttatcccgtc atcactggcc gacatttttt ataaggactc
     4741 tcccctgccc ctgcaaaaat atacttaggg cctgctgatt atatcacagt gccgaatatt
     4801 cgttgttcgt ttgcagccaa gtacattcac ggataaaatc cacccaatgc gccacaatac
     4861 gctgtgctga aaatatcaaa gggattacat agttgtagca gcccaacccg attaatttaa
     4921 ccaggttgag cactaaaacg gtggtggcta tccatgattc gcttgtttgt gtgagccttg
     4981 ctttaatacg gttcatccca tatgccgtct tggcttgtcc aaacttccct tcaattggat
     5041 ttctttcgcc cggacttatg tggtcttcca ctgccttcgg ccttcccaat ggcttcgcat
     5101 gaagcgtgat tccaagcaac ttgagtgccg ccctgttagt tctattgcaa tatattttgt
     5161 cagccaacac atctgctgga taatacccaa atcgttccct gtacttctct accgaagcca
     5221 ttagtcgtgt cccctcatta aatgcgttcc acgagagatc atccagaaaa gcaaaaccat
     5281 tcatcaatga gacatttatt tttgctccaa attcaacata cgcattggac tttcctctga
     5341 caatagggcg tacgtgcggc tgatgtatgt ttactatgcg atcttcaatg ctgtgaacat
     5401 gctcggaaaa cattttttgt tgttgctcat agacagtatt gatcacaaga agatatttgt
     5461 aatccttttg ttggagcgga ataacaggat acacatctaa aagcaggttg atgcttttaa
     5521 gattttttct cagatagctc aattgctttt ttattgcttt gcgtatctct ttttttgatt
     5581 tattcttttt ctgcgcggta tgcaggtata acttccgcgc ttctttcctg tcggttctcg
     5641 gtttggattc atgaagctgc ggcaaataca atttatcaat cagctgttca gatttttccc
     5701 gggcatcgtt cagtaaattt aaatctgttg gataagctat gtcctgcggg catgccgtgg
     5761 cgtcagtgat aagttttccc ctgtgttctt tcaatgaagc atcctttgct atttgtttta
     5821 ttgaagtatt ctcttcattc gtagtgtcgc caccatctcc tgaacctgct tcatcttctg
     5881 gaaagttgtc ctctggtttt tttgacaacc caagaatttt ttcattgatc gcattgattt
     5941 gctcaatgcc catccgcttc ctgaactcta cgaacaacga gggatcgaaa ggctcttcat
     6001 tactgaaact actgtaccca ataaagtatt gcatgtacat gttttcctgg atctgcaata
     6061 ctgtttctcg atcgctcatg ttgcaaatat gtttcagaat catggcccca atggctactc
     6121 gcggatttat cccttctgca ccccgctgac tattgttaag ttgagcttga taggtggtta
     6181 ccagcaaatc ccatggaatt tgtcgggata gttttaccca ccggttatca gcatgcaatt
     6241 tttgagagaa gggtgtctca aatccctcga gagtcatctg gttttggctg atgtatgcat
     6301 tcttggatgc acggtttttg caagcttttg ccatattttc atgcatttta tgtactacaa
     6361 atatgcagat aattcttgaa aataaagcgc tttagaattt ttcagcagac cctacttacc
     6421 atgaaaccca atgggatatt cctttgccgt ctttctgttt tttcaaaaaa gatgatgtgt
     6481 accgcgacat agaacaggaa aattctattg aaggacagtt taaaaaaaac ggcaacgaat
     6541 aataccagct actgattatt gggcatatta gttcaggcac cctgcatttt tgcctgataa
     6601 ccccgttcat ttcttttctc gatataattg ataaacgagc ggtttaccac tttgttcccg
     6661 ccgggagtgg gataatcgcc cgaaaaatac cagtcaccgg tattgcccgg acaggcagca
     6721 tgaagatcgc tcaccgactg gaaaatgatc tgtacccggg ccctgatatc ttcaggcacc
     6781 agcaactgtg ttattttttc ggcaatgcgt tccggagtaa aaggctcata gatttccttc
     6841 acataattga caattttttc tttcggcaga tgctcctgtg ctttgcattt tttatacaca
     6901 cggttgataa catcgtcctg tttggtttcg tgcaacagcg caatagctgc cctgaaagcg
     6961 ataaaatcat ttaacttggc catatcgatg ccgtagcagt cgggataacg aatctgcgga
     7021 gccgaagagg ctataataat ccttttcgga ttcaggcggt caagcatgcg gataatgctc
     7081 tcccgcaggg tggtaccacg cacaatagaa tcgtctatta ctacaattgt atccgcataa
     7141 tccctgataa ttccataaga aacatcgtac acatgggcca ccaggtcgtc acggtcttta
     7201 tcctgcgtaa taaacgtacg gagtttcata tctttcacgg ccaccttttc aaaacgggga
     7261 tgaaaagcca ggattttttc cagttcttta gctgtgatat cgttcccggc ttgcattatt
     7321 ctgtcttttt ttacgccgtc gcaaaacttt tccagagcac ccatcatgcc ataatacgcc
     7381 acggcagccg tattcggaat ataggagaac accgtgtttt ccaaatcgta atcaatggct
     7441 ttcaggatgg aaggcaccag aaaactgcca agttttttac gttcaaggta gatatccttg
     7501 tcggtacctc tcgaaaagta tatacgttca aaagagcatg acattttttt tgccggctgt
     7561 acaaactgcg atagcctgta gctgccgtct tttttaacta tcaatgcatt acccggcaaa
     7621 atttcgttga ctttttcgat ggctacattg aaggtggtca tgatggccgg gcgttccgat
     7681 gccaccacca ccacctcatc atcggcataa taataagcag ggcggatacc ggcagggtcg
     7741 cgcattacaa aagcatcgcc gtggcctatc ataccggcca gcacaaagcc gccgtcccaa
     7801 tctctggcag cgcgggtaag cagccgttgc acatcaagat ctgccgctat cagcgccgac
     7861 acatccttct tgctgacgcc ttgtttttta tatgactgat aaagatcttc gttttcctca
     7921 tcaagaaaat gcccaatctt ctcaagaata gtaacggtat cggtagtttc gacagggtgc
     7981 tggccgagac tgatcagctt gttgaacaac tcgtccacat tggttagatt gaaatttccg
     8041 gccaatacga tatttttggt ttcccagctg ttttcccgga taaacggatg gcaggtctcg
     8101 atgctgtttt taccaaaagt gccgtagcgc aaatggccga ggtacagttc cccgacaaag
     8161 tcagcgttgt gtttcagcca gttcacatcg ctgtacagcg atggttttgt tttccgtaat
     8221 tctttcagcg cggtataagc cggagaaaag atatccgtga taggttggga agagttggat
     8281 cgtgaacggt tgataaaacg cataccaggc tccaggtcaa gttttataca ggctattcct
     8341 gcgccgtcct gtccgcggtt atgctgtttt tccatcatca gcatcagctt gttcaggccg
     8401 taaagggggg tattgtattt taaacggtaa tattcgagag gttgcaataa tcggataaag
     8461 gcaataccgc attcgtgttt tatcgactca ctcatacagt tgttttttta caaaattatt
     8521 gtttcgcaaa caattatttc aaattttgca gatgagttat taacaaaaga tactgaaact
     8581 cagaagtttt tattgtcaaa attca
//
LOCUS       DAOSFP010000053         8573 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7036_length_8573_cov_3.737732, whole genome shotgun sequence.
ACCESSION   DAOSFP010000053 DAOSFP010000000
VERSION     DAOSFP010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7036_length_8573_cov_3.737732"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="PLI16_02620"
     CDS             complement(<1..726)
                     /locus_tag="PLI16_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83483.1"
                     /translation="MMKIVLISLIAIVCDPLIILGQGSFNAYFGSLHSHTSNSDGAGE
                     PEDAYNYARFTAGLDFLAVTDHLEQIGNVPLIDPEWSDCQDAADNATVVGTFVGIAGW
                     EWGSPLHGHVNVFNTADIIDDVGNLWYTTDLPEFYNWVLNHSPAFAQFNHPGEETYFT
                     NWNSFAYVSTALDNVFSLIEFQSVAQASGFYELSLNNGWRLSPVWNQDNHSADWGTKN
                     DCRTGIWATTLSRPALFEAIMAGR"
     gene            895..1224
                     /locus_tag="PLI16_02625"
     CDS             895..1224
                     /locus_tag="PLI16_02625"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH83484.1"
                     /translation="MTKNLKTIYDEEKRIDFNQLEDAANRLKIMAHPIRLAILIMITS
                     KKEMTVTEIYEALSLQQAAVSNHLKMMKIHDMLYSRRDGKNTYYYVNIKSMKDLYTLL
                     SLDKRPA"
     gene            1583..2203
                     /locus_tag="PLI16_02630"
     CDS             1583..2203
                     /locus_tag="PLI16_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83485.1"
                     /translation="MISLVACKKDKNTIHIEGKVYDPNTAAYVSDARVTIAASKLSSG
                     GIFSAGYADIATAVSDAGGNFVFTFEEEKFGGFQIRVEKENYFSTFKEISTSEIVAGT
                     TYTPTLNILPVCFIDLKIRNAAPYDTNDLVFYSFTSGFLNGSGCCGNTITYGHGLYYA
                     DTVLCKTHGNQNVTLSYNVRKEGITTLHTKTLYCTAFDTTYFVIDY"
     gene            2215..3003
                     /locus_tag="PLI16_02635"
     CDS             2215..3003
                     /locus_tag="PLI16_02635"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOH83486.1"
                     /translation="MKAIQDIVIIGAGNVAVNLAHAFRKAGKNIRCIYSRDAARARHL
                     ANSVGADFTDKFEMLPNKADLFLIAVTDSAIAEVADKLSSTEGIVAHTSGSVAISVLN
                     KRSAGQGVFYPLIHLSSENIIDFTTVPLCLEATTEKTMQALWQLAGTISEKVFSVTSD
                     DRKILHLAAVFACNFTNLNYIIAEEILKKNGLPFELLHPLIAGLARKIQTASPSTIMT
                     GPAIREDYEVIAAHLNMLESIPHYQEIYQMLTKTIIQKKKNNEL"
     gene            2990..3511
                     /locus_tag="PLI16_02640"
     CDS             2990..3511
                     /locus_tag="PLI16_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOH83487.1"
                     /translation="MMNYKELLKDINTMIFDYDGVLTDGTVITTERGEQYRTGYVRDG
                     YALHLAIKKGYNVAIISGGTSDSISKRLETLDIKDIFTHVSNKIETFHRYLEQKKVSA
                     SQVLYMGDDIPDYKVMQLAGLPVCPADAAEEIKSISKYISSYKGGHGCVRDIIEQLMK
                     IQGTWMNDDAFEW"
     gene            complement(3894..5498)
                     /locus_tag="PLI16_02645"
     CDS             complement(3894..5498)
                     /locus_tag="PLI16_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOH83488.1"
                     /translation="MKKPVFLMLLACMISTVMIHAQSLPVKSKIKDVTVFFNGAQITR
                     HGDIALPAGQSTVIFEDLTQYINAQSIQVKGEGNFDILSVVHQINYLKMQEKTPEVKK
                     IEDSIQILNRMLTSQQTILAVYQQEETMLLANKDIGGEQTGVKIAELKEATEYFRTRL
                     LDIKTNQMNIQDKINKIQKNITTLNNQLQTWNARVAEPSSEILVTVSAATPVTASLSL
                     DYYVTNAGWTPTYDLRAVNVSSPVGLDFRANVYQTTGADWSNVNLTLSTGNPMQNGSK
                     PLLNPWYLSFAYPYGMVTSQQTARKEAAPMAKVAADEGVNYEQQARSTADYTVVNETQ
                     TNIEYAISIPYTIPADGKNHMVQIQKYNLPATYEYYCAPKLSQDVFLLARVTGWDKYN
                     IISGEMNLFFEGTYVGKSYINARTTADTIEFSLGTDNNITVKREKLQDFSSSKLIGLN
                     KKETFTYQISIRNKKKQNIDIVIDDQVPLSSDKNIEIELLDISSAIYDKDYGKITWKY
                     NLKPAETKEIRLSYSVKYPKDKYLNL"
     gene            complement(5594..6607)
                     /locus_tag="PLI16_02650"
     CDS             complement(5594..6607)
                     /locus_tag="PLI16_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83489.1"
                     /translation="MAKKVTSKKISSQKPGPEKPKKEKPAQEMEHNIRYRIGDKGVFI
                     GLIVVWGIVGIFGFFTLTQPQWLVNLSAPGRTEEALTNITAGNSYLYQGKAENSELKF
                     KKALANYQKALEIDPENYEATANMGITYLYLNQLEEAKKTFEHCLKKDTIHSHHTYTY
                     LADIYERQGEITKALEYYQKSLIKHPNPAYPLRKAGLLSQRLFNFEDAIKYLKQSIEA
                     TNSFENEYRATLMDASYKLLKDNDTLNLAIIDKELHKNNYTEVLSRYDTTIFKQVLKN
                     SKDLGYAYMYLADAYFSKTQFDSAYACYQKSSQYHPALVEKFKNKQAFAFENTTKNFV
                     GIR"
     gene            complement(6609..7817)
                     /locus_tag="PLI16_02655"
     CDS             complement(6609..7817)
                     /locus_tag="PLI16_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOH83490.1"
                     /translation="MKKIPVVFINFFFFSLLVILTPFLMLQNFLQNAIGRASRAAVDL
                     DFISIPYVVIIAILVLILFFYLSRKILSKRRLLYLGFIVLFVSVGQMTADYYFNHKFY
                     DLQHNWHYIAYGLYAYVAYRRFSYKPTPTHKVIFRIFITGLVISVFDEFIQVFISNRI
                     FDLGDCGKDIWGTMTGIIFVYFYLEDGKDFPNFRLRNKKIKEYYTNPFSLLCLEVIFA
                     YVFLYVASQISDSIYKFQVLLITLLLFIIIFLLIHAGVNKIARWVIRGALLIIIIFVF
                     VAARFGTSGIQAKSPGFINYNGIPLIYFDYMIFPDGTFRTVDKKTMFTLRDKQKIEHL
                     GSDIVLVGTGMKGQGGLGWNDILTTELSYNTFKSKIYQLIKLPNRQACETYNRLKKEN
                     KKILFIIHNN"
     gene            7961..>8573
                     /gene="surE"
                     /locus_tag="PLI16_02660"
     CDS             7961..>8573
                     /gene="surE"
                     /locus_tag="PLI16_02660"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOH83491.1"
                     /translation="MMQKPLILVTNDDGVAAPGIRFLISVMRELGEVVVLAPDKPQSG
                     MGHAITVSSPLRMQKITVEDHYKEYSCSGTPVDCVKLAVDKILHRKPDLLVSGINHGL
                     NSSVNIIYSGTMSAALEGAMVNIPSAGFSLDDYAYHADFSHTAEYIKVICRNLMHHGL
                     PPKTCLNVNFPKNVKKLKGIKVCRQANAHWKEEFNERKDPHGRN"
BASE COUNT         2435 a         1788 c         1821 g         2529 t
ORIGIN      
        1 cctgcccgcc ataatggctt caaataaagc aggccggctt agtgtggtag cccaaatgcc
       61 ggtgcggcaa tcatttttgg tgccccagtc agcagaatgg ttgtcctggt tccataccgg
      121 tgaaagacgc cagccattat tcaatgacag ctcataaaaa cctgaggctt gtgccaccga
      181 ctgaaattca atcagtgaaa aaacattgtc cagggctgtg cttacatagg caaaactgtt
      241 ccagttggta aaatatgttt cttctcccgg atggttaaac tgtgcaaatg ccggtgagtg
      301 gttaagcacc caattgtaaa attcgggcag gtcggtggtg taccacaaat tgccgacatc
      361 atcaataata tcggcggtgt tgaacacatt cacatgccca tgcaggggcg atccccattc
      421 ccagccggct attcccacaa aagtaccgac caccgtagca ttgtcagccg catcctggca
      481 atccgaccac tccgggtcga tgagaggaac atttccaatc tgttccaggt gatctgtaac
      541 ggcaagaaaa tccaaacctg ccgtaaaccg ggcataatta tacgcatctt ccggttctcc
      601 ggccccgtcg gaattagagg tgtgcgaatg caggctgcca aaatacgcgt taaagcttcc
      661 ctgccccaat attatcaaag gatcacacac tatagctatt aaacttatga gaacaatttt
      721 catcataaaa attatttggc acagtttttt catgtggtgg tcaaaaattg ataaaaaatt
      781 aaaactaaga atattttttt ataaatacat atatatatta aaacttttgt tttacaaatt
      841 ctctcctgaa aataaattaa caatactata aaaataaaaa aaaccagaca aatcatgaca
      901 aaaaatctta aaacaattta tgatgaggaa aaacgaattg acttcaatca gctagaggat
      961 gctgccaatc ggttaaaaat catggcccat cctatacgtc tggctatact gattatgatt
     1021 accagcaaaa aggaaatgac cgttaccgaa atttacgagg ccctttcctt gcaacaggcg
     1081 gccgtatcaa accatctgaa gatgatgaaa attcatgata tgctgtattc ccgaagagac
     1141 ggaaaaaaca cctattacta tgtgaatatc aaatccatga aagatcttta cacgctcctc
     1201 agtctggata agcgccctgc ttaaccccag tttacccaat aacgtacttg gtcctttttg
     1261 tataaggcac gccaggcgtc cccgcttgaa ctggcagtca aaagagcacc tctgaaaact
     1321 tcaaatttct ttgttcgtca ttgttcttca aattctcata tacgctcaga ctatgcggta
     1381 ttcagaactt cgactgcccc ggactttttt atagagcggt ccgatcaatg ttattagagg
     1441 cccccataag ttaatcaggc tttctaagct tactattttt tcttccgatt attttatagt
     1501 gattgcgtaa ttttgtaaat tgcaacagaa tttattaatt atgtgttctc ctgtattccg
     1561 gataattatt ttttgtgttg gaatgatttc ccttgttgcc tgtaaaaaag acaaaaacac
     1621 tatacacatc gaaggtaaag tttatgatcc caacacggct gcttatgtaa gcgatgccag
     1681 ggtaaccatt gccgccagta aactctcttc aggaggtatt tttagtgcgg gatatgccga
     1741 tattgcaacg gctgtcagcg atgccggcgg taattttgtt tttacttttg aagaagaaaa
     1801 gttcggcgga tttcagataa gagttgaaaa agaaaattat ttttctacat ttaaggaaat
     1861 ttccacctct gaaatagttg ccggcaccac ttatacgcca acgctgaata ttctgccggt
     1921 atgtttcatt gacttgaaaa taagaaatgc tgcgccttat gataccaacg acctggtttt
     1981 ttattcgttc acatcagggt ttctcaacgg ttcgggatgt tgtggcaata ccatcactta
     2041 cgggcatggg ctttattacg ccgacacggt attatgcaag acgcacggca accaaaatgt
     2101 aaccttgtct tacaatgtca gaaaggaagg aataacgacc ctgcacacca aaaccttgta
     2161 ttgcacggca ttcgacacca cgtattttgt gattgattac taataacagg caacttgaaa
     2221 gcaatacaag acatagttat aataggcgcc ggaaatgtag cggtaaatct ggctcatgct
     2281 ttcaggaaag caggaaaaaa catacgttgt atttacagcc gtgatgctgc cagggcccgg
     2341 catcttgcga actcagtggg cgctgacttt accgataagt ttgaaatgct tccaaacaaa
     2401 gcagatttgt ttctcattgc cgttaccgac agtgctattg ccgaagtagc ggataaactg
     2461 agcagcacgg aaggtatcgt ggctcacacc tccggttcgg tcgccatctc cgttttgaac
     2521 aaacgttccg caggacaagg tgtcttttat cctttgatac acctttcttc cgaaaatatc
     2581 attgatttta caacagtgcc cttatgtctt gaagccacca ccgaaaagac catgcaagca
     2641 ctatggcagt tggcgggcac gatttccgaa aaagtgtttt ccgtaacatc ggacgataga
     2701 aagattttac atcttgcagc ggtatttgcc tgtaatttta caaatctcaa ctatattatt
     2761 gctgaagaga ttctgaaaaa aaacggcttg ccgtttgagt tgcttcatcc gctgatagcc
     2821 ggattggcca ggaaaataca aactgcctcg ccatcaacca taatgacagg gcccgctata
     2881 cgtgaagatt atgaggtaat agccgcccac ctcaacatgc tggaaagcat tccgcattat
     2941 caggaaattt atcaaatgct taccaaaacc atcatacaaa aaaagaaaaa taatgaacta
     3001 taaagaactg ttgaaagaca tcaatacaat gatttttgat tacgacggtg ttctaaccga
     3061 tggtaccgta atcaccacag aaagaggcga acaataccgc accggctatg tgcgagatgg
     3121 ttatgccctg cacctggcaa taaaaaaagg atataatgtt gccattatca gcgggggtac
     3181 atccgattcc atcagcaaac gcctcgaaac gcttgatatt aaggatatct ttactcatgt
     3241 cagcaataaa attgaaactt tccatagata cctggaacaa aaaaaagtgt cggcttcgca
     3301 agtgttgtat atgggcgatg atatccctga ttataaagtg atgcaactgg ccggtcttcc
     3361 agtttgtccg gccgatgcag ccgaagaaat aaaatccata tccaaataca tttcttctta
     3421 taagggcggc catggttgcg tgcgcgatat tatagaacaa ctcatgaaaa tacaaggcac
     3481 ctggatgaac gacgacgcgt tcgaatggta aaggaacact gctgaaattc caaatttcgc
     3541 aggtcatctt tggtattcaa atattcctgt acgttaagta cacaacagtt ttcagcacct
     3601 ttgcctcatc ggattttttt tgaaagttat ccgatcaaca tttataaaga catccccaaa
     3661 aagctgttgc tcataaattt caatatgctt accccgatta ccgcgcaaat caccctgtga
     3721 ttgaaatttt acttttacag gctgtcaaca ccgaattacg caacagacgt ggtctgttgc
     3781 gtaattcggg ttgccttgcc ggctttgcgc tgtagctatt attagctgga aaaatattcc
     3841 ggaataacgt gaatttttag tttaaaattg aagaacagga gcattgatga atactacaaa
     3901 ttcaggtact tgtccttagg gtattttacc gaatacgaaa gccttatttc tttggtttcg
     3961 gccggtttca ggttatattt ccaggtgatt tttccataat ccttatcata tatagcggat
     4021 gagatatcca acaattcaat ttctatattc ttatctgacg atagcggcac ctgatcatca
     4081 attacgatat cgatattttg ttttttcttg ttgcggatac ttatttgata ggtgaaagtt
     4141 tcttttttgt tcaggccgat taatttggag ctggaaaaat cctgtaattt ttcgcgtttg
     4201 acggtaatgt tgttatctgt tcccagcgaa aactcaatgg tatcggctgt tgtcctggca
     4261 ttgatgtatg atttgcctac ataggtgcct tcgaaaaaca ggttcatttc gcccgaaata
     4321 atgttgtact tgtcccatcc tgttaccctg gcaagcagaa agacatcctg gctcagtttt
     4381 ggcgcacagt aatactcata agttgccggc aggttgtatt tttgaatctg caccatgtga
     4441 ttctttccat cggcgggtat ggtataaggg atgctgatag cgtactcgat gtttgtctgg
     4501 gtttcgttca ccacagtgta atcggccgta ctgcgggctt gctgttcata gttcactcct
     4561 tcatcggcgg cgactttggc cataggcgcg gcttctttcc tggctgtttg ctggctcgtc
     4621 accatcccgt aagggtaggc aaatgaaaga taccacgggt tcagcagcgg tttggagccg
     4681 ttttgcatgg gatttcctgt tgacagggtg aggttgacat tgctccagtc agcgcctgtg
     4741 gtctgataga catttgcccg aaaatcaagg cctacaggac tgctgacatt gacagcccgc
     4801 aggtcgtagg tgggagtcca gccggcattg gtaacgtaat agtccaggct aaggctggca
     4861 gttaccggcg tcgccgcact gacagttacc agaatctcgc tgcttggttc ggcaaccctt
     4921 gcattccagg tctgcaactg gttgttcaaa gtggtaatat ttttttggat tttattgatc
     4981 ttgtcctgaa tattcatctg gttggttttt atatcaagca ggcgggtgcg gaaatactct
     5041 gtggcttctt tgagttcggc gattttcacg ccggtctgct cgcctccaat gtctttgttg
     5101 gcgagcaaca tggtttcttc ctgctgatat acagccagga tagtttgttg tgaagtgagc
     5161 atcctgttaa gaatctggat agagtcttca atttttttca cctccggggt tttttcctgc
     5221 attttaaggt aattgatctg gtgtaccacc gataagatgt caaaattgcc ttcgcctttc
     5281 acctgtatgc tttgcgcgtt gatgtactgt gtcaggtctt cgaaaatcac ggtgctttgt
     5341 ccggcaggca gggcaatgtc gccatgccgg gtgatttgtg ctccgttgaa aaataccgtt
     5401 acgtctttaa tttttgattt aacgggcaat gactgcgcgt ggatcatcac ggtagaaatc
     5461 atgcatgcca gaagcattaa aaaaactggt tttttcatat tgtttgtggt tttaaggttt
     5521 tttgaataga gaagtaaaaa caaattttcc acaaatttta tgaaaaattt ttttctgagg
     5581 aaagttcagt cagctacctg atgccgacaa agttttttgt cgtgttctca aaagcaaaag
     5641 cctgtttgtt tttaaatttc tcaaccagcg cgggatggta ctggctgctt ttctggtaac
     5701 aggcatacgc cgaatcaaat tgcgttttgc tgaaataggc atcagccaga tacatatagg
     5761 cataccccag gtctttgctg tttttaagga cttgtttaaa aatggtggtg tcgtacctgc
     5821 tgagcacctc agtgtaattg tttttatgaa gttctttatc aatgatggcc aggttgaggg
     5881 tatcattgtc tttaagtaat ttataactgg catccatcag ggttgcccgg tattcgtttt
     5941 caaaactgtt ggtggcttca atagattgtt tcaggtattt tatggcatct tcaaaattaa
     6001 atagtcgctg gctcagtaaa ccagctttgc gcaaaggata agcagggttg ggatgtttaa
     6061 taagggattt ctgatagtat tccagcgctt tggtaatttc gccctgccgt tcgtagatat
     6121 cggccaggta ggtgtaagta tgatgactgt gaatagtatc ttttttcagg caatgctcaa
     6181 aagttttttt ggcttcttcg agctggttga gatacaaata agtaatcccc atattggctg
     6241 tggcttcgta gttttcaggg tcaatctcga gggctttttg atagtttgcc agcgcctttt
     6301 tgaatttcag ttccgagttt tcggcttttc cctgataaag ataggaattt cccgcggtaa
     6361 tattggtaag ggcttcttcg gtacgtcccg gtgccgacaa attgacaagc cactgtggtt
     6421 gtgtcagggt aaaaaatccg aaaatcccca ctattcccca aacgactatc aggccgataa
     6481 aaacaccctt atcaccaata cggtaacgga tattatgctc catttcctgt gccggttttt
     6541 ctttttttgg tttttccgga cctggtttct gtgatgatat tttttttgat gtaacttttt
     6601 ttgccatatc agttgttatg tatgatgaaa agaatttttt tgttttcttt tttcagtctg
     6661 ttgtacgttt cgcatgcctg ccggttgggc agttttatta actgataaat tttactttta
     6721 aatgtgttgt acgacagttc ggttgtcagt atatcgttcc atcccaggcc gccctggccc
     6781 ttcatgccgg tacccaccag cacaatatcc gaaccgaggt gctcaatttt ctgtttgtca
     6841 cgcagggtaa acatggtttt cttatccact gtcctgaatg tgccgtcggg aaatatcata
     6901 tagtcgaaat atattaaggg aatgccgtta tagttgataa atccgggact tttggcttgt
     6961 attcccgatg tgccaaaacg agctgctaca aaaacaaaaa tgataatgat caggagggct
     7021 ccgcgtatga cccatcgggc gattttgttt actccggcat gtatcaataa gaaaattatg
     7081 atgaataata ataaggtaat cagcagcacc tgaaatttat atattgaatc agaaatttgc
     7141 gaggccacat acagaaagac ataggcaaaa atgacctcaa gacataatag tgagaatggg
     7201 ttagtgtagt attctttaat ttttttgttt cgcaggcgga agttcgggaa atcctttccg
     7261 tcttccagat aaaagtatac aaagatgatt cctgtcattg tcccccatat gtccttgcca
     7321 caatcgccca gatcgaaaat tctgttggaa ataaaaacct gaatgaactc atcgaaaacc
     7381 gaaatgacca ggcctgtgat aaaaattcta aaaatgactt tgtgcgtagg cgtgggtttg
     7441 tatgagaaac gtcggtatgc tacgtaggcg tagagtccgt aggcaatata atgccagttg
     7501 tgctgcaaat cgtaaaattt atggttaaaa taataatccg ccgtcatttg tcccacactg
     7561 acaaaaagca caataaaacc aaggtaaagc agccttcttt tgctcagaat cttacgcgag
     7621 agataaaaaa acagtattaa cacaagtatg gcaatgataa ccacataggg aatactgata
     7681 aagtcaagat caaccgctgc tctcgaagcc ctgcctatgg cattttgcaa aaaattctga
     7741 agcatcagaa acggggtgag tatcaccagc agcgagaaaa aaaagaagtt aatgaatacg
     7801 accggtattt ttttcataag ctaaattatg atgcgataaa gctaataaaa aaattcaaaa
     7861 tacaaggtgt gtgcatttaa aagtacatat ttttgtttcg aagggaagca tcaattgtaa
     7921 aaaaaagtat cgtaaaaaat attaattttg cataaaaaac atgatgcaaa aacctttgat
     7981 acttgtaact aatgatgacg gagtggctgc tccgggtatc aggtttctga tcagcgtgat
     8041 gcgcgaactg ggcgaagtgg tggtgctggc tcccgacaaa ccgcagtcag gcatgggcca
     8101 cgctattacg gttagttcgc ctttgcgcat gcaaaaaatc actgtcgaag accattataa
     8161 agaatattcc tgcagcggca cgccggtgga ttgtgtaaaa ctggctgtgg ataaaattct
     8221 gcaccgcaaa cctgatttgc tggtttcagg aatcaaccat ggattaaact cctcggtcaa
     8281 tatcatttat tccggcacga tgtcggcagc cctcgaaggt gccatggtaa acataccttc
     8341 cgcaggtttt tcgctcgacg attatgccta tcatgctgat ttcagccata cggcggaata
     8401 tatcaaagtg atctgccgca accttatgca ccacggcctg ccgcccaaaa catgcctcaa
     8461 cgtcaacttc ccgaaaaatg taaaaaaact caaaggcata aaagtttgcc gtcaggccaa
     8521 tgcacactgg aaagaagaat ttaacgaacg caaagacccc catggccgta act
//
LOCUS       DAOSFP010000054         8563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7049_length_8563_cov_4.372120, whole genome shotgun sequence.
ACCESSION   DAOSFP010000054 DAOSFP010000000
VERSION     DAOSFP010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8563
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7049_length_8563_cov_4.372120"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2825)
                     /locus_tag="PLI16_02665"
     CDS             complement(<1..2825)
                     /locus_tag="PLI16_02665"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83492.1"
                     /translation="MNHINITPVITSAVRRGLLVVAVLICNNFFVNAQVQKAFTQRTS
                     VYTPTKTIYNIKGDFTMIGNTNMTLEDYEDDRLNSNNVMIKVDADSDPSTINSSMATL
                     VFSSENGAIPECSNIIYAGLYWTARTNSSVTTTEKRAIKLKGPGSGSYTSFNAAVTDI
                     QYPGDNYMYAGYIEVTDYVRAHGVGQYWVADMALTTGNGGSTGYYGGWGMIVVYENSK
                     MKWRDVTIFDGYAYVVGNTSVYYDLPISGFTTVQSGQVDMKLGLMAGEGDVGIYGDYF
                     QIRKVSDATWMSLNHGGNATNNFFNSSIYTGGNTRNPNLLNNTGMDVSMFNIPNPSNT
                     VITNNQTSTTFRYGSTQDTYIIFCIAMAVDAYVPTPEGLISVNSIGGSAPSVPPVALP
                     GQEIEYKVEIKNKGTEPIINTRIVIPMPYSASYVSCSGVVNFTPLPTPNNLYFDPTLG
                     ATGSIVWDFGTLPLPADPNTVLATLTFRINVTTNCKILMNPNCPPDVPIYGTFSGTGA
                     TSSTSFTGYHFIQGFQTSGLCQGEPIYDPIHVAIDAADYIAEHCQGTPAEIALTYCNV
                     GTTIPVTEVLGNFPPGSRFYDSYPVTSSSIEYTISNPFPATSGTHTYYAVVPGIIDCY
                     FIFTITVTNITTVPSVSPLSYCLGQAAVPLTATATNPSYLLYYYTSLSSSEIPQMSIT
                     PSTAAVGSTTYYVAEGVSASCISPNRAPLVVTVNLLPVVTVTGNSPVCVGSNLFLVEN
                     GGEATSWSWTGPNGFTSTNDTAIILGAGLANDGIYTVTISNGICSNSASINADVSMDP
                     IVVEDCPDDITVCADTMYGGELGAFVDWDIPDFSMNCMAGLPGSSNNFVMGFELPEVK
                     WACWEFNKVQRIGPTVVNLWQSTGTGNPHILTPSVYIEPPLSVEMDIICEPGRNFTWS
                     VYLVRGVTESFAGSVYVNTSGHYSVAIP"
     gene            complement(2860..3369)
                     /locus_tag="PLI16_02670"
     CDS             complement(2860..3369)
                     /locus_tag="PLI16_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83493.1"
                     /translation="MMAFRRCSLFILFFAVLYFLTVQNLPAQNIKHFDEVLNALSTSD
                     QDQAKADASHFKSLIYDVQPRLYIDKNGFKNSSDSRPVCVTTDPQSYELLYETNPLFE
                     QVELITVNILNARDLNFIFDMTRLKSFSKLKYVYFLCNYNCDPALIRLQWPNLAGSVI
                     VFYQISLPE"
     gene            complement(3805..4470)
                     /locus_tag="PLI16_02675"
     CDS             complement(3805..4470)
                     /locus_tag="PLI16_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH83494.1"
                     /translation="MKNIKVLLVDDHQIILDGIQTMLSSEPDISICGACITCKDAFEY
                     ILKHRPDVIITDINMPEMSGIELIKKIKSYNITAKILVLSMFASADYIQNAVNAGANG
                     FLMKQNATRDELMTAIGQLMANKNYFNSEVANTIVTGIKPAFDDHDQFVKTHEKNLLS
                     KRELQVLKLFAEGHTNKEISEKLFISLRTVETHKTKIISKLNMKSYVDLIKYAIRNKL
                     CDL"
     gene            complement(4479..7055)
                     /locus_tag="PLI16_02680"
     CDS             complement(4479..7055)
                     /locus_tag="PLI16_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH83495.1"
                     /translation="MSKKNIKILAIDDKTENIFLLKELIVDSFRGTVFISALNGSQGI
                     KLCKSQKPDVVLLDITMPDMNGFEVCKIMKADEEIRHIPVIMITAALVGSEMRVQALD
                     SGADAFLSKPIDISEFKAQIRAMLRIKESEDHKRLEHHLLESEVKKRTRALNKELAER
                     RQAELKLKAAYNNLKKNQKITESLMNELKAEVKERLQAEIKVLHHLEEQKIISDFSSR
                     LVSLNQLEEVYQFVGQRVFENIDNAYVVVTQFNYHARSIGVKWVYGMEKFRRKIKALF
                     GFDPFLKELAVADIYYKPEELSAFRSQQFFEPGRGNNGLHFLFAFLLDKNLCNEMQKV
                     LGVKKIYTMGFTFGQKVYGGLSILMKEDVPLPQIKLFETLISETSVVLQRLFVEEKLK
                     ISEKLYRSLVITSPDGIMMLDIKGFPLAINKKCYEIFGFAEGDTVFSKRNIFNFISPV
                     DRERCRINFKKRIRGEPMPNQEYMAISKDGSEFPIDVNSSAIMDENEKPYALISVIRD
                     ISQRKRAEKLLIESEEKYRLLAHNMIDILSVFDRSGKIIYINNSIFKHLGFKPANVIG
                     MSFMDFLYMEDLPSVRQLLKRNLAMYPTQPNIVLRIRKKNKGYVWFDTNILFLKNNLG
                     RLTGFQCVSRNISEKIKSDNRLEAERKKVMSALIDGQELERQRLSMELHDGLGQRLAG
                     IKIKLENSADLSLADTRQAITAIKSEFRDVIDEIRYMSMNVSPTILTDLGLVTSLSML
                     GKQFRENNSIDFEYTVMGEFDNFSEQKAFSIYRIVQEGLNNIAKHAHATEAKLALIEK
                     EGNSLIVIEDNGNGFNKEMIARSKGNGLSNMRQRALLLNGTMSIESGINSGTVVMIKI
                     PK"
     gene            complement(7202..7696)
                     /locus_tag="PLI16_02685"
     CDS             complement(7202..7696)
                     /locus_tag="PLI16_02685"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOH83496.1"
                     /translation="MKIENIKALIFDWGDTIMRDFPEKPGPMHTWDKVEWIPGAENLL
                     KTVSKNFIMVIATNAGASDTVAMIKALRRVGADKYFSHFYSSKELGYDKPDVRFFTVI
                     AEHIGITPEHCLMTGNLYEKDIVGAKASGMKTVLFNEKKSEGDFSQADAVIHSMEELI
                     SLLG"
     gene            complement(7745..>8563)
                     /locus_tag="PLI16_02690"
     CDS             complement(7745..>8563)
                     /locus_tag="PLI16_02690"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOH83497.1"
                     /translation="GMAEHAARYRDFAAYFAGAGFAVFANDHRGHGKTAGSVENTGYI
                     AGNNSWKSILADTKLVNDHIHKILPNVPVIVYGHSMGSFYARAFLELYSGDLAGVVLS
                     STAWHPALVLGFGLTVAKIQCFTSGEKHKSKLLDKLSFGAFNKKFKPRATPFDWLSRD
                     VDVCKKYTADDYCGWVASASFFRELFRLLKFIHRSALYQLTDTKIPVLIFSGEDDPVG
                     DFSKGPRKMYEFLSKKGFTNVKLLLYPQGRHEMHNELNRLEVYKNMADWLAERL"
BASE COUNT         2548 a         1851 c         1598 g         2566 t
ORIGIN      
        1 ggaatggcca cagaataatg tccgctggta ttcacataaa cactgccggc aaaagattcc
       61 gtcacccccc tgaccagata tactgaccag gtgaaattac ggcccggttc acaaataata
      121 tccatttcaa ctgacaacgg aggctctata tatacagaag gagttaatat gtgcggattc
      181 cctgtccctg tagactgcca taggttcaca acggttgggc caattctttg cactttatta
      241 aattcccaac atgcccactt aacttcaggc agttcaaagc ccatcacaaa gttgtttgag
      301 ctaccaggca gccctgccat acagttcata ctgaaatcag gaatatccca atccacaaaa
      361 gcgcctagtt ctccaccgta catagtatct gcgcaaaccg tgatatcatc agggcaatcc
      421 tcaaccacga taggatccat actcacatcg gcattaatac tggcactgtt tgaacaaatt
      481 ccgttgctga tagttacagt ataaatccca tcatttgcca atcccgctcc caggataata
      541 gccgtatcgt tggttgatgt aaaaccattt gggcctgtcc aactccagga ggtagcttcg
      601 cccccatttt ctaccaggaa aagattactt cccacgcaaa caggagaatt gcctgttact
      661 gttacaaccg gtagcagatt aacagttaca accaaggggg ccctgttggg actgatacaa
      721 gatgccgaca caccttcggc aacataatag gtggtggaac cgacagccgc agtggatgga
      781 gtaatagaca tctgcggaat ttctgaagaa gaaagagatg tataatagta taataaatac
      841 gaaggatttg ttgccgtagc ggtaagtggt actgctgcct gcccaaggca ataggataac
      901 ggagacacag atggcactgt cgttatattt gttacagtaa tggtaaaaat aaagtaacaa
      961 tcaattattc ccggaacaac agcataatat gtatgagtac cggaagtagc tggaaaagga
     1021 ttactaatgg tatattctat tgaagaagaa gttacgggat aagagtcata aaatcgggaa
     1081 cccggcggaa aattacccaa aacttctgtt acaggtatgg ttgtgccaac attacaatag
     1141 gtaagtgcta tttctgccgg agtgccctgg caatgctccg cgatatagtc agcagcatca
     1201 atagctacat gaataggatc atagatcggc tccccctggc ataaaccaga ggtttgaaaa
     1261 ccctggataa agtggtatcc cgtgaagctg gtcgagctgg tagctcccgt cccgctgaaa
     1321 gtaccataaa taggaacatc tggcggacaa tttggattca tcagaatttt gcagtttgtg
     1381 gttacattta ttctgaaagt aagagttgcc agcacagtat tgggatcggc gggtaaaggc
     1441 agggtgccga aatcccagac aattgatcct gttgcaccca gggtcgggtc aaagtaaaga
     1501 ttatttggtg ttggtaaggg tgtaaaattg acaacgccgg agcaactgac atacgaggca
     1561 ctgtatggca tcggaatgac aattctcgta ttgatgattg gctctgtccc tttgttcttt
     1621 atttcaacct tatattctat ttcctgaccg ggtaaagcaa ccggcggaac cgacggggcc
     1681 gaccctccta tgctgttaac agaaatcaat ccctccggcg taggaacata agcatctact
     1741 gccatggcaa tacaaaatat aatataggta tcctgagttg atccgtacct gaaagttgtt
     1801 gaagtctgat tattggttat cacagtattg ctgggattag ggatgttgaa catgctcaca
     1861 tccatccctg tattgtttaa taggttcgga ttgcgggtat tgccaccggt gtatattgaa
     1921 ctgttaaaaa agttgtttgt ggcatttccg ccatggttga ggctcatcca cgtagcatcg
     1981 ctaactttac gtatctggaa ataatcccca taaatgccca catctccttc accggccatc
     2041 aatcccaatt tcatatctac ctgcccggat tgtacagtag taaaaccact tataggtaag
     2101 tcgtaataca cagaagtatt tccaaccaca taggcatagc cgtcgaaaat ggtaacatcc
     2161 cgccatttca tttttgagtt ttcatacacc actatcatgc cccagccacc ataatatccg
     2221 gtagaaccgc cattaccagt ggtaagtgcc atatcggcaa cccagtattg tccgactcca
     2281 tgcgcacgca cataatcggt tacttcaatg taaccagcat acatataatt atcaccagga
     2341 tactgaatat ccgtcactgc cgcattgaaa gaagtataac tgcctgatcc agggcctttc
     2401 aattttatgg cgcgtttctc tgtagtagtc acagagctat ttgttctggc tgtccagtat
     2461 agccccgcat atataatatt tgaacattcc ggtatggcac cattctcgct cgaaaatacc
     2521 agagtcgcca tggaggaatt gatggtcgac ggatcgctgt cggcatcaac ctttatcatg
     2581 acgttgttgc tgttgagcct gtcatcttcg taatcttcaa gtgtcatatt ggtgttacct
     2641 atcatggtaa aatcgccttt gatattatat atcgttttgg taggcgtata aactgaagta
     2701 cgctgagtaa atgctttctg tacctgggca tttacaaaaa aattgttgca aatcaacact
     2761 gcaacaacta acaagccacg tctgactgcg cttgtaataa ccggggtaat atttatatga
     2821 ttcatggcaa ggggggtatt gaatttgtac tacataatat tattcgggta aggatatttg
     2881 ataaaaaaca attacgctgc cggcaaggtt tggccattgc agcctgatta aggcaggatc
     2941 gcaattataa ttgcataaaa aataaacata ttttaatttt gaaaaacttt taagtcgtgt
     3001 catatcgaaa atgaaattga gatctcgcgc atttaagatg ttcactgtta ttaactcaac
     3061 ctgctcgaac agcgggttgg tttcgtataa aagttcatag gattggggat cggttgtaac
     3121 acaaacgggg cgtgaatcgc tgctgttttt aaatccgttt ttgtcgatat acaacctggg
     3181 ctgaacatca taaatcagac ttttaaaatg ggaggcatct gcttttgcct gatcctgatc
     3241 agaggttgac aacgcattta aaacttcatc aaaatgtttg atgttttggg caggtaaatt
     3301 ctgtactgta agaaaataca aaacagcaaa aaacaaaata aaaagactgc atctcctaaa
     3361 tgccatcata gaaaaaacaa ccttattttg cttacaaaaa aaagtgttaa aatttttcat
     3421 atttatggtt ttgttattta tacaacttat tgattattta gaacttacat tttttattaa
     3481 tttctttatt tattatatta taaggacata aaggtataat taattatacc gtcatgaaac
     3541 agtgattaac tgaaaaccat acgcgaaatg cttaaaacgg ggattagtga atcacttatt
     3601 tttcgtaata gtttgagtac aaaaaagtga atcaataatg acggaaaaaa gattttgact
     3661 ttttacgaaa aacaaaccat ataacttaca caacaatgaa agaagtacaa ctttttttcc
     3721 aaaatacggt aaatgaggga ataatacaaa cgcgactgat ttcttagata actcaatcac
     3781 gataaactat gagagaatga caaattataa atcgcaaagt ttgtttctaa tggcatactt
     3841 gatcaaatct acataagact tcatgtttaa cttggaaata atttttgttt tatgagtttc
     3901 aacagtacgc aagctgataa acaacttttc agaaatttct ttgttggtgt gaccttcggc
     3961 aaataatttc aaaacctgaa gttctctctt tgaaagtaaa tttttttcat gtgttttgac
     4021 aaattgatca tggtcatcaa aagctggctt tataccggtg acaatggtgt tagccacttc
     4081 agaattaaaa tagtttttat ttgccatcaa ctgaccgatc gctgtcatca attcatcacg
     4141 agtggcattt tgcttcatga gaaatccgtt agcccctgca tttactgcat tttggatata
     4201 atctgcagat gcaaacattg ataatacaag tatttttgct gttatattat aagatttaat
     4261 tttttttatt aattcaatcc cactcatttc gggcatattg atatccgtga taataacatc
     4321 cggtctatgc ttaagaatat attcaaaagc gtctttacat gttatacatg ctccacaaat
     4381 tgaaatatct ggttcagaag aaagcatagt ctggatacca tctaatataa tctgatggtc
     4441 atcaactaac agtactttta tatttttcat ttaataagtt atttcgggat ttttatcatg
     4501 acaactgttc cggaattgat accggattca atactcatag ttccgttaag caacaaggcc
     4561 cgttgtcgca tattggataa tccgttgcct ttgcttcggg caatcatttc tttattaaaa
     4621 ccgttgccat tatcttcgat aacaatcaac gagttgcctt ctttctcaat caaggccagt
     4681 ttagcttccg tagcatgtgc atgtttggca atattattta ggccttcctg cactatccgg
     4741 taaattgaaa aagctttttg ctccgaaaag ttatcaaact cgcccatgac agtgtattca
     4801 aaatctatgc tgttattttc cctgaactgc ttacccagca ttgacagcga tgttaccagt
     4861 cccaggtctg taaggatggt aggactgaca ttcatcgaca tataccttat ttcgtcgatg
     4921 acatccctga attctgattt tattgcagtg atagcctgcc gggtatctgc tagtgacagg
     4981 tcagccgaat tttccagttt tattttaatg cctgccagcc gttgccccag gccatcatgc
     5041 aattccatcg acaagcgctg ccgttcgagt tcctgtccgt cgattagcgc cgacattact
     5101 ttttttcttt cggcttccag ccggttgtcc gatttaattt tttccgatat attgcgcgaa
     5161 acgcactgaa atcctgttag ccggccaaga ttgtttttca gaaaaagtat gttggtatca
     5221 aaccagacat atcctttgtt ttttttgcgt atcctgagta ctatattggg ttgcgtagga
     5281 tacatcgcca ggtttctttt cagcagttgc ctgacgctgg gcaaatcttc catgtacaga
     5341 aaatccataa aactcatgcc aatgacgttg gcaggtttga atcccaaatg tttaaaaata
     5401 gaattattta tataaattat ttttcccgac ctgtcgaaca cagaaagaat atctatcata
     5461 ttatgagcca gcaaacgata tttctcttcg ctttcaatca acaatttttc agcccgtttt
     5521 cgctgactga tgtctcttat cacacttatt agcgcataag gtttttcgtt ttcatccatg
     5581 attgcagagg aattcacatc gataggaaat tcgctgccgt ctttagaaat agccatatac
     5641 tcctggttgg gcattggctc gcctctgatc cttttcttaa aattaatacg gcatcgttcg
     5701 cggtcaacag gcgaaataaa attgaaaata ttcctctttg aaaacacagt atcaccctct
     5761 gcaaatccaa aaatttcata acatttttta ttaatggcaa gaggaaatcc tttaatatcc
     5821 aacatcatga tgccatcggg cgaagtaatg accagggagc ggtacaattt ttcacttatt
     5881 tttagttttt cttccacaaa caagcgctga agcaccaccg atgtctcact aatcagcgtc
     5941 tcaaacagtt tgatttgcgg caggggcaca tcctctttca tgagaatgct caacccacca
     6001 taaacctttt gaccaaaagt aaagcccatg gtgtaaattt ttttgacacc aagaaccttt
     6061 tgcatctcat tacataaatt tttgtccagg aggaaagcaa acaaaaaatg caggccgtta
     6121 ttgcccctgc cgggttcaaa aaattgctga ctgcggaatg ctgacagttc ttcaggttta
     6181 taatagatat cggctactgc cagttctttc aaaaagggat caaaaccaaa caaagccttt
     6241 atttttctcc tgaatttttc catgccgtaa acccatttta cgccaatgct tcgtgcatga
     6301 taattaaact gggtaacaac aacataagca ttatcaatat tttcaaaaac tctttgaccg
     6361 acaaactgat aaacctcctc caactggttt aaacttacca atctacttga aaagtcgctg
     6421 ataatctttt gttcttctaa gtgatgaaga acttttatct cagcctgcaa ccgttctttt
     6481 acctcagctt tcagttcgtt catcagggat tcggtaatct tctggttctt tttaagatta
     6541 ttatatgcgg ccttgagttt tagttcggcc tggcgtcgtt cggcaagctc tttgttaagc
     6601 gctctggtac gtttctttac ttctgattcg agtaaatggt gctcaagcct cttatgatct
     6661 tcagattctt tgattcgcaa catggcccgt atctgagcct taaattcact gatatcaata
     6721 ggtttgctta agaaagcatc ggcaccggaa tccagcgcct gcacccgcat ctcgctaccc
     6781 accaaggcgg cggtaatcat gatgacagga atatgccgga tttcctcatc agccttcatt
     6841 atcttacaaa cctcaaatcc gttcatgtcg ggcatggtaa tatcaagtaa aaccacatcc
     6901 ggtttttggc tcttgcaaag tttaatgcct tggcttccgt taagggctga aataaaaacc
     6961 gttccccgaa aactatcaac aataagttct ttcagcagaa aaatattttc agtcttatcg
     7021 tcaattgcca atatctttat gttctttttc gacataaaaa aaatattaaa acttctttaa
     7081 aagaagcaaa aaagcacacc aaaacaaaaa atacggcacg ggcaaattca cagcagaaca
     7141 aatatataaa ataattgtta atttaccaaa ttaaattaaa aaaactacac caaaaaataa
     7201 cttatcccaa aagcgaaata agctcttcca tagaatgaat cactgcatca gcctgagaga
     7261 agtccccttc agactttttt tcattgaaga gtactgtttt catgccactg gccttagctc
     7321 cgacaatatc tttttcgtat agattacctg tcatcaggca gtgttcggga gtaatgccga
     7381 tgtgctcggc aatgacggta aaaaaacgca catcaggttt gtcatagccc aactccttcg
     7441 aagaataaaa gtgtgaaaag tatttatcag cccctaccct tctcaaggcc ttaatcatgg
     7501 ctacggtatc cgaagcccct gcattggtgg caattaccat aataaaattt ttactcaccg
     7561 tcttcagcag attctcagct cccggtatcc attccacctt atcccacgta tgcatgggtc
     7621 ctggcttttc gggaaaatca cgcataatgg tatctcccca gtcaaatatt aatgctttta
     7681 tattttctat tttcatagtt ttaagccatt agtgtttcta tcattacatt gtcagtgttt
     7741 tattttataa acgttccgca agccagtcgg ccatgttttt atagacctcc agcctgttaa
     7801 gttcgttgtg catttcgtgc cgtccctgtg gatacaaaag cagctttaca ttggtaaatc
     7861 cttttttact tagaaattca tacattttcc gcgggccttt cgaaaagtcg cccaccggat
     7921 catcctcgcc cgaaaatatc agcaccggaa ttttcgtatc cgtcagctga tataatgcag
     7981 aacgatgaat gaatttgagc aacctgaaca attccctgaa aaaagaggca gaagccaccc
     8041 acccgcaata atcgtcggca gtatattttt tacagacatc cacatcgcgg ctcagccagt
     8101 cgaatggggt ggcacgcggt ttgaattttt tgttgaatgc cccgaaagac agtttatcca
     8161 acagtttgct cttgtgtttt tcgccacttg taaaacactg aatctttgct accgtcaatc
     8221 caaaaccaag cactaaggca ggatgccagg cagtgctcga caataccacg cctgcaagat
     8281 ccccggaata aagttccaaa aaagcccttg catagaaaga ccccatgctg tgtccgtaaa
     8341 caattacggg aacattgggt aaaatcttat gaatatgatc atttacaagt tttgtatcgg
     8401 caaggatgct tttccaactg ttgtttcctg cgatataacc cgtattttcc accgaaccgg
     8461 cagttttccc atgtccgcga tggtcgttgg caaacacggc aaaaccagcc cctgcaaaat
     8521 aggccgcaaa atcacgatac ctcgccgcat gttcggccat tcc
//
LOCUS       DAOSFP010000055         8552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7068_length_8552_cov_4.255855, whole genome shotgun sequence.
ACCESSION   DAOSFP010000055 DAOSFP010000000
VERSION     DAOSFP010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7068_length_8552_cov_4.255855"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..347)
                     /locus_tag="PLI16_02695"
                     /pseudo
     CDS             complement(60..347)
                     /locus_tag="PLI16_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
     gene            527..1588
                     /gene="aroB"
                     /locus_tag="PLI16_02700"
     CDS             527..1588
                     /gene="aroB"
                     /locus_tag="PLI16_02700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOH83498.1"
                     /translation="MKKTGKYLFSDTDKNIFRKYLQEKIESGCSLFILMDENTKKFCK
                     SKLTDDFPILKNAGEIVIKSGEQHKTLETCNHIWLKLIAHHADRHSLLINLGGGVITD
                     LGGFAAASYKRGINFCNIPTTLLGTIDAAIGGKTGLDYQGMKNVIGCFSFAEKTYINP
                     EFLISLPERQIKSGYAELLKIALISEKLLWKQLQPQDIKDLSSLTTLIKKAVLLKDKI
                     VQQDPLEDHTRKTLNFGHTIGHAIESYSLEHDKKPLLHGEAVAIGMICESWISKKKKM
                     LSGSELDEIIFKLIRCSFSEKYKIDFDGLSAFLINDKKNSGLKINFSLLQGIGKAQID
                     NFIDEKMISEAVMFYNNLI"
     gene            1632..2885
                     /locus_tag="PLI16_02705"
     CDS             1632..2885
                     /locus_tag="PLI16_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOH83499.1"
                     /translation="MRPVIIHSDAGNIKASIKLPASKSISNRLLVIKSLCDAPFRINN
                     LSSSDDTRILGDCLNNKEHYRVFDVKDAGTPLRFLTAVFALTPGNRMLTGSPRLQERP
                     LAPLVQALNELGAIICFTDKHGFPPLVINGNDITGGSIAIDASISSQFISALLLIAPA
                     LPKGIELTLKNNTASVSYIRMTLKLMEHFDIVSKWFQDVITIRPQKYQPTDCTVESDW
                     SAAAFWFEIAALAENANIELHGLSSDSLQGDAAITEIFKNLGVQSTFENDTLRLTKCD
                     AATNFLQYDFFETPDLFPAVVATCAAKNISFRFTGLKNLAIKESDRVKAMVNELQKFG
                     FRFSSPDENTLTFEAGTPLVSAREISCATYNDHRIAMALAPLSLLGKKIFLDQPSCVS
                     KSYPSYFEDLKNSGFHVKFSENDYF"
     gene            2902..3666
                     /locus_tag="PLI16_02710"
     CDS             2902..3666
                     /locus_tag="PLI16_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOH83500.1"
                     /translation="MQIYKQISEIIRQNQEAVLCIITDTTGSSPRKAGSKMIVFPDKK
                     ITGTIGGGSIEFQAISDALDVMKTGVPTKKTYQLEEDLAMHCGGTVELFFDPIKSHPD
                     LYIFGAGHIGRVVARFARDFGFRVTLFDQRDKIFDEFDSAAYTCINENYFAAIEKATF
                     NSGTFCVIVTPKHLYDENILIKLLRKPHAYLGMIGSPAKVAVARKNLIEKEGFTEQEI
                     NMVDMPIGIKFNAVTPEEIAISITAKLIDVKNSLNK"
     gene            3668..5107
                     /gene="xdhA"
                     /locus_tag="PLI16_02715"
     CDS             3668..5107
                     /gene="xdhA"
                     /locus_tag="PLI16_02715"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HOH83501.1"
                     /translation="MNKVSFVFNDQIQEIDFANSPYTPTTSVLKYLRSLNGHKGVKEG
                     CGEGDCGACTLVLAELNGDGLRYKAVDSCLVFLPMIHGKQLITVENLADAKGDLHPVQ
                     QAMIDLYASQCGYCTPGFVMSLFALYKSPVKPVNEVILDSITGNLCRCTGYLPIIEAA
                     AKACSKKEKDIFSIREKQTINLLKQINNQDVIVITTPKQKYYKPFKLTDVLELKRKHP
                     DALIISGNTDTGLRVSKKHELLTEIIDISAVEELKSITETKQSLSIGAGVSMEEVKLA
                     TEKLFPALHKMLSVFGSKQIRNLATIGGNIGSASPIGDTLPVLMACNAVVALSSHNDS
                     RVLGIQDFIKGYRTTDLRKDEIICSIGIPFPEKDQIIKSYKISKRKDLDISTCSAGFM
                     LELNKNDHTVKNIILAYGGMAALTRRATKTEEFLAGKKWSRTTVEKAAAILYQEFTPL
                     SDARSGEEFRRLAARNLLWKFYEETTSKK"
     gene            5109..7433
                     /gene="xdhB"
                     /locus_tag="PLI16_02720"
     CDS             5109..7433
                     /gene="xdhB"
                     /locus_tag="PLI16_02720"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdopterin binding
                     subunit"
                     /protein_id="HOH83502.1"
                     /translation="MKKTSETILHHDSAALHVTGKAVYVNDMEVSGQTLYGKVVYSPH
                     AHAQIIRCDVSKARMVKGVHAVLTYKDIPGANQMGPVTHDEPCLAEKEVTAIGQAVAL
                     IAADNEETALEAEKLIAISYKPLKPILDIKTAQTADNRIAPTRTIEMGDIAKAFKKSA
                     HVLEGELHSGAQEHWYLEAQSALCVPGERDEMMVYASSQNPSETQAIVAEVLGISKKE
                     VVCEVRRMGGAFGGKETQGNHVAAWAALMANSTRKAVAVHLFRDDDQKITGKRHPFQS
                     TYKIGFDHEGRINAYTVDLNADAGHASDLSMAILERAMLHADNAYYIPNVKITATAWR
                     TNHFSNTAFRGFGGPQGMAVIECAIDRIARFLKKDATEIRVKNFYTENKGNITPYHQK
                     IDNIRLQKIYARLIKTAEYAKRKKSIDQFNKNNEFVKKGIALTPIKFGISFTTSFLNQ
                     AGALVNIYRDGSVLVNHGGTEMGQGLNTKMLQVASAELGISAKYIKVNATNTSKVPNT
                     SATAASSGSDLNGAAVKNAVDILKNRLIPVAFELLTNKFPDRKFSKNRITFCEDLVFD
                     KIYPKQALPFYEVIDKAYLKQVSLSATGFYKTPDIYFDREKGIGKPFHYFAYGMAVAE
                     VELDILTGSHKLARVDIVHDVGDSLNEPIDTGQVCGAFIQGVGWVTTEEIKWDDTGRL
                     LTCSPDTYKIPSAQDIPEIFNIQLLENAANNNTIRKSKAVGEPPFMLAFSVWMALRYA
                     VAAVRNHELDPDLAIPATNEKIVLAVEKLLKTKN"
     gene            7599..8267
                     /locus_tag="PLI16_02725"
     CDS             7599..8267
                     /locus_tag="PLI16_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOH83503.1"
                     /translation="MEMEYNTGRPLLNITEYGRNVQKMIEYTMTLEDRDERNKAARTI
                     VAIMATLCGNQKQDTAEFKQKMWDHLFMMSDYKLDVDAPFPKPDPETKENELFKCSYP
                     NKFIKYRQYGKNIENMISRAIELEEGEEKSHLVRYIANHLKKLYISWNRDSVSDEVIL
                     EHLAVLSEGKLKLNENFKLENTRDLIAVNTRKKTSGSPSDNKNNKYRDKNWKKKKQQQ
                     QQQQ"
     gene            8333..>8552
                     /locus_tag="PLI16_02730"
     CDS             8333..>8552
                     /locus_tag="PLI16_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOH83504.1"
                     /translation="MNTFEVIGGKSLNGEIVPQGAKNEALQVISAVLLTPEKVTISNI
                     PDIVDVNRLIGLLADLGVEVEKIAPDTYT"
BASE COUNT         2697 a         1975 c         1821 g         2059 t
ORIGIN      
        1 atctgtgtaa aaccttcttc ttccgcaatc gttgcaaatt cagggtacaa ttctgtttat
       61 tcttcgtttt caccgttggc agcctccacc aggttatcgg cagtgcctcc tatcattcct
      121 gccggatagg tagccgtaat ttcaacgggt cctccttcca gaaaacgaaa gaatatcttg
      181 gcatgacact cttcctgcag ggacgtcagg gcaaaaattt cggcaatctg ctcataacca
      241 tcctttttgg cttgctttgc aaaaaaagtg taacggtttt tggcctgcga ttcaccggca
      301 aaggctttta gcaggttctt ttcagtacgg gatcctttta tgttcataaa tttaatgttt
      361 tgacgtgaaa aatcttccgc ggcggataag cggcggatta atgaaaaata cggcaaatgg
      421 tgaaaagaaa tatgttaccg gcatgatggt atatcatttt tcatttggta acaaaattac
      481 ataaccttgg caaattgtgt atctttatgc cgtgtaattt ttttttgtga aaaagactgg
      541 taaatatctt ttctccgaca ctgataaaaa tattttcaga aaatatctgc aggaaaaaat
      601 agaatcgggc tgcagcctgt tcattttgat ggatgaaaac actaaaaaat tttgcaagtc
      661 taagctgact gatgattttc ccatcctgaa aaacgccgga gaaattgtga taaaaagcgg
      721 cgaacagcac aaaacgctgg aaacatgcaa ccatatctgg cttaaactta ttgcgcatca
      781 tgccgacagg cattcactgc tcatcaacct tggcggcgga gtgattaccg atctcggcgg
      841 ttttgcagcg gcctcgtata aaagggggat caatttctgc aatatcccaa cgacgctgct
      901 gggaacgatt gacgcggcca taggagggaa aacaggactg gattaccagg gaatgaaaaa
      961 tgtcattggt tgtttttcct ttgcagaaaa aacctatatc aaccctgaat ttttgatttc
     1021 actgcccgaa cgccaaataa agtcgggata tgctgaattg ctgaaaatcg ccctcatctc
     1081 ggaaaaactt ttatggaaac agttgcagcc acaagatatc aaagaccttt cctccctgac
     1141 aacactgata aaaaaagctg ttctcctgaa ggacaaaata gtacaacagg atccattgga
     1201 agatcatacc cgtaagacac tgaattttgg ccataccata ggccatgcca tagagtcgta
     1261 ttcgctggaa cacgacaaaa aaccattact tcacggcgag gcagtagcta tcggtatgat
     1321 ttgcgaatcg tggatatcaa aaaagaaaaa aatgctgagc ggttccgaac ttgatgagat
     1381 aatttttaaa ctcatcaggt gttcctttag cgaaaaatac aaaattgact tcgatgggtt
     1441 gtcggccttt ttaataaacg acaaaaaaaa ttccggtctg aaaattaatt tctcattgct
     1501 ccagggcatc ggcaaggcac agattgataa tttcattgat gaaaaaatga tttccgaggc
     1561 tgttatgttt tataataatc tcatataaac gaaaagcaaa actctccact tccaaaagaa
     1621 atcgcagata aatgaggccg gttattattc atagcgatgc aggcaatata aaggccagta
     1681 ttaagttgcc cgcatccaaa agtatcagca accgcctgct ggtaattaaa tccttatgcg
     1741 atgccccttt cagaataaac aatctttcat catcggacga tacccggatt cttggtgatt
     1801 gcctcaacaa caaggagcat tatcgggtat ttgacgtaaa agacgccggc actcccctgc
     1861 ggtttctcac ggcggtattt gcactaactc cgggaaacag gatgctcact ggctctcctc
     1921 ggctacagga aaggccgctg gcacctctgg tacaagcctt gaatgagctt ggggcaataa
     1981 tttgttttac cgataaacac ggttttcctc ccttggtaat caatggcaat gacattaccg
     2041 gcggcagcat agccatcgat gcatccatca gcagccagtt catatctgcc ctgctgctca
     2101 ttgcacccgc gctgccaaag ggaatcgaac tgacacttaa aaacaataca gcttcggttt
     2161 cttacatacg catgactttg aaactgatgg agcacttcga catcgtttcc aaatggtttc
     2221 aggatgtgat aacaatacga ccacaaaaat atcagccaac agattgcacc gtcgaatccg
     2281 actggtcggc agccgccttc tggttcgaaa tagcggcact ggccgaaaac gccaatatcg
     2341 aacttcacgg attgtcgtcc gattcgctgc agggtgatgc tgcaataaca gagattttta
     2401 aaaaccttgg tgtgcagtcc acctttgaga atgatacact tcgtttgaca aaatgtgatg
     2461 ccgcaaccaa ctttcttcag tatgattttt ttgagacgcc cgacttattt cctgccgtgg
     2521 tagccacctg tgcagcaaaa aatatttcct tccgttttac cggccttaaa aaccttgcaa
     2581 taaaagaatc ggaccgcgta aaagccatgg ttaacgaatt acaaaaattt ggctttcgtt
     2641 tttcctcacc cgacgaaaac acgctgacat tcgaggctgg tactcccctg gtttctgcaa
     2701 gggaaatttc ctgtgcaaca tacaacgacc accgcattgc catggccctg gcgccgctgt
     2761 cgttgttggg aaaaaaaata ttcctggatc agccttcctg cgtttcaaaa tcatatccct
     2821 cctattttga agacctgaaa aactcaggat tccatgtgaa gttttctgaa aatgattatt
     2881 tttgaacgac aattttttat tatgcagatt tacaaacaga tttcggaaat catcaggcaa
     2941 aatcaggaag ctgtgctttg tatcataacc gataccacag gttcttcgcc gcgcaaagcc
     3001 ggctccaaga tgatcgtatt tcccgacaaa aaaatcaccg gcaccatagg aggcggaagc
     3061 atagaatttc aagcaatctc tgatgcgctc gatgtcatga aaaccggcgt tcccacaaaa
     3121 aaaacctatc aactcgaaga agacctggcc atgcattgtg ggggtaccgt agagttgttt
     3181 tttgatccga taaaatccca ccccgacctt tacatttttg gagcaggaca tattgggcgc
     3241 gtagtagcca gatttgccag ggattttggc ttcagggtta cattatttga ccagcgagac
     3301 aaaatttttg atgaatttga ctcggcggca tacacctgca tcaatgagaa ctattttgct
     3361 gcaattgaaa aagcaacttt caactccggt acattttgtg taatcgtaac tcccaaacac
     3421 ctgtacgatg aaaacatact gataaaactc ctcaggaaac ctcatgcgta cctgggaatg
     3481 attggaagcc cagccaaagt tgctgtcgcc cgcaaaaacc tcatcgaaaa agaaggcttc
     3541 acagagcagg aaatcaacat ggtggatatg cctatcggca taaaatttaa tgccgtaaca
     3601 ccggaggaaa ttgccatcag cattaccgcg aaactaatag atgtaaaaaa cagtctaaac
     3661 aaataatatg aacaaagtaa gctttgtgtt taacgaccag atacaggaga ttgattttgc
     3721 aaattcgcca tacacaccaa ccaccagcgt attaaaatac ctgcgttcgc taaacgggca
     3781 caaaggagtg aaagaaggat gcggtgaagg cgattgcgga gcctgcacgt tggtgctggc
     3841 cgaattgaat ggcgatggtt tacggtataa agcagttgac agttgccttg tgtttttgcc
     3901 catgatacat gggaaacaac tgattaccgt agaaaaccta gctgatgcca agggagactt
     3961 acatcctgta caacaagcca tgattgattt atatgcttcc caatgcggct attgtactcc
     4021 cggctttgta atgtcgttgt ttgccctgta taaaagtcct gtaaaacctg tcaacgaggt
     4081 aattctggat tccatcacgg gtaatctctg ccgatgcacc ggctacctgc ccatcatcga
     4141 ggccgcagca aaagcatgca gtaaaaaaga aaaggatatc tttagtatca gggaaaaaca
     4201 aactataaat ctactgaaac aaatcaacaa tcaggatgtt attgttatca ctactccgaa
     4261 acaaaaatat tacaagcctt tcaaactgac tgatgtcctc gaattaaaaa gaaaacaccc
     4321 tgatgcgctg attatttcag gaaataccga caccggtctg cgggtatcaa aaaaacatga
     4381 actgttaact gaaattattg atatctcagc agtagaagaa ctgaaatcca tcactgaaac
     4441 aaagcagagt ctttctatcg gtgccggtgt cagcatggaa gaagtgaaac tggcaacaga
     4501 aaaactgttc cctgccctgc ataaaatgct gtcggtattt ggctcaaaac agatcaggaa
     4561 cctggccacc ataggaggca atatcggatc ggcctcgccc atcggcgaca ccctgcctgt
     4621 gctgatggcc tgcaacgcgg tggtggcatt gagcagccac aacgattcga gggtgctcgg
     4681 catacaggat tttatcaaag gttaccgcac caccgacctg cgtaaagacg aaatcatctg
     4741 ttccatagga atcccttttc cagagaaaga tcagattatt aaatcataca aaatatccaa
     4801 gcgcaaagac ctcgacattt caacctgcag cgccggattt atgctcgaac tcaacaaaaa
     4861 cgaccatacc gtcaaaaaca tcatcctcgc ctatggagga atggctgcat tgacacgccg
     4921 tgccacaaaa acagaagaat ttctggcagg aaaaaaatgg tcaagaacca ctgttgaaaa
     4981 agcggcagct atactctatc aggaattcac acctttatcg gatgcccgtt caggcgaaga
     5041 attccgccgc ctggccgcac gaaatctttt atggaaattt tatgaagaaa ccacttcaaa
     5101 aaagtagtat gaaaaaaact tcagagacaa tcctccacca cgatagtgca gcattgcatg
     5161 taaccgggaa agccgtatat gttaacgaca tggaagtcag tgggcaaaca ctttacggaa
     5221 aggtggttta cagcccgcat gcccacgcac agatcatacg ttgcgatgtc agcaaagcaa
     5281 gaatggtaaa aggtgttcat gcagtgttga cgtacaagga tatccccggc gccaaccaaa
     5341 tgggccccgt aacccacgac gaaccctgcc tcgccgaaaa agaggttact gcaatcggcc
     5401 aggcggtagc gctgatagcc gctgataatg aggaaacagc cctggaagcc gaaaaattga
     5461 ttgccatatc ctacaagccg ctaaaaccca tactggatat aaaaaccgcc cagacggccg
     5521 acaacaggat tgcgcccaca cgcaccattg aaatgggcga tatagccaaa gcatttaaaa
     5581 aatctgctca tgttctggag ggcgaattgc acagcggcgc acaggaacat tggtacctgg
     5641 aagctcaatc agccctgtgt gtcccgggcg aaagagacga gatgatggta tatgcctcaa
     5701 gccagaatcc atcggaaaca caagccattg ttgctgaagt gctgggcatc tcaaaaaaag
     5761 aagtggtttg tgaagtaagg cgtatggggg gcgcttttgg cggtaaagaa acacagggca
     5821 accatgtggc agcctgggct gcactgatgg ccaactcaac ccgcaaagcc gtcgccgtgc
     5881 acctgttccg cgacgatgac cagaaaatta ccggcaaacg ccatcctttc cagtccacct
     5941 ataaaatcgg gttcgaccat gaaggccgca tcaacgccta caccgtggac cttaatgccg
     6001 atgccggcca tgcctccgat ctgagtatgg ctatcctgga acgggctatg ttacatgccg
     6061 acaacgccta ctatatccct aatgtaaaaa ttactgccac agcctggcgc actaaccact
     6121 tttcaaatac ggctttccgt ggattcggag gcccgcaggg catggccgtc atcgaatgcg
     6181 ctatagaccg catagcacgt ttcctgaaaa aagacgccac cgaaatcaga gtaaaaaact
     6241 tttacaccga aaacaaaggc aacatcactc cgtaccacca gaaaattgat aatataaggc
     6301 ttcaaaagat ttatgcccgg ctaatcaaaa ccgctgaata cgcaaaaaga aaaaaatcca
     6361 ttgatcaatt caacaaaaac aatgaatttg taaaaaaagg aatagcgcta acaccaatca
     6421 aattcggcat ttcattcact acctcttttc tgaatcaggc aggagccctg gtgaacattt
     6481 accgcgatgg cagcgtgctg gtcaatcatg gcggcactga aatgggacag ggactgaaca
     6541 ccaaaatgct ccaggtggct tcagccgagt tggggatttc tgcaaagtat atcaaagtta
     6601 atgctaccaa tacatctaag gtgcccaaca catctgccac ggcggcatcc agcggatcgg
     6661 acctgaacgg cgctgccgta aaaaatgcgg ttgacatatt aaaaaaccgt ttgataccgg
     6721 tggcatttga gttattgaca aacaagtttc ctgacaggaa attttcgaaa aacagaatca
     6781 ctttttgtga agaccttgtg ttcgacaaaa tatacccgaa acaagcactg cctttttatg
     6841 aggttatcga caaagcatac ctaaagcaag tcagcctgag tgccacgggt ttttacaaga
     6901 ctcccgacat ttatttcgac agggagaaag gcattggaaa accctttcat tattttgcct
     6961 acggaatggc ggtggcagag gtggaactcg atatacttac cggctctcat aagcttgcaa
     7021 gagttgacat tgtgcatgat gttggcgaca gcctgaacga acccatagac acaggacagg
     7081 tttgcggagc atttattcag ggtgtaggct gggttactac cgaagaaata aaatgggacg
     7141 acaccggacg ccttttgacc tgttcgcccg acacatataa aattccttct gcgcaggata
     7201 tcccggagat attcaacata caactgcttg aaaacgccgc taataataac accatacgaa
     7261 aaagcaaagc cgtgggcgag cctcctttta tgctggcatt ctcggtatgg atggccctgc
     7321 gttacgcagt ggctgcagtt cgtaatcatg aattagatcc ggacctggct attcctgcaa
     7381 caaatgaaaa aatcgtgttg gccgttgaaa aacttttgaa aacaaaaaat taagcccttg
     7441 ggaaaaaggc cttgaggaaa cgttagttgt tgaaaacaca gcaggtttgc atatgactat
     7501 atgcagatgc gccgcaagcc tttctcaaag ccttgactct ctaaaaattt tcttatcttt
     7561 gctgaaaaat aaactaattt tatatttaaa ctcacagaat ggaaatggaa tataataccg
     7621 gcaggccttt gctgaatatc accgaatatg gcagaaatgt ccagaaaatg attgaataca
     7681 ctatgactct tgaagacagg gacgaacgca ataaagctgc acgtacaatc gtggctatca
     7741 tggctacgct ttgtggcaac caaaaacaag acactgccga attcaaacaa aaaatgtggg
     7801 atcatctttt tatgatgtcg gattacaaac tggatgttga cgcacctttt cccaaaccgg
     7861 accctgaaac aaaagaaaat gaactgttta agtgctctta cccgaacaaa ttcatcaaat
     7921 accggcaata cgggaaaaac atcgaaaaca tgattagccg tgccatcgaa ctggaagaag
     7981 gcgaagaaaa aagccacctg gtgcgttata tcgccaacca cctgaaaaaa ctttacatca
     8041 gctggaaccg cgactccgtt tctgacgagg ttatcctgga acaccttgct gttctctctg
     8101 aaggcaagct gaaactgaac gaaaacttca aacttgaaaa tacacgcgac ctgattgccg
     8161 taaacacccg caaaaaaacc tccggcagtc cgtccgacaa caagaataac aaataccgcg
     8221 acaaaaactg gaagaagaaa aaacaacagc aacaacaaca gcaataatca tcagccgtcg
     8281 ataacaataa atttgtatcc tgaacactaa tggttttaaa aaataaaatc acatgaatac
     8341 atttgaagtt ataggaggaa aatccctgaa cggtgaaatt gtaccgcagg gcgcaaaaaa
     8401 tgaagcttta caggttatca gcgccgtgct gctgactcct gaaaaagtta ccatcagcaa
     8461 catccccgat attgtggatg tcaacagact cataggcctg ctggctgacc tgggcgtgga
     8521 agtagaaaaa atcgcaccgg atacttatac gt
//
LOCUS       DAOSFP010000056         8485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7144_length_8485_cov_3.308660, whole genome shotgun sequence.
ACCESSION   DAOSFP010000056 DAOSFP010000000
VERSION     DAOSFP010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7144_length_8485_cov_3.308660"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..2192
                     /locus_tag="PLI16_02735"
     CDS             264..2192
                     /locus_tag="PLI16_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH83505.1"
                     /translation="MKILLISPGTPDEIDNKIIRSIPYLNAKAFIAPHAVATVAALTP
                     EEHDVVIHDEYIRGPSEDYFRGKSFDIIGISITSNQLKRSLEIAQACKKFCPDSLVVV
                     GGIGVEMLIYKQKENIDVVFHGEAEETWPRFLEDYKNRTHQKVYKSVIKPDMSKVPPP
                     RWELIKEDILLYNSVSLQTTRGCPFDCSFCDVIYTYGRKPRSKTIEQVIDEIKKLHSL
                     GVQMVFIADDNFAGDKKYVKELLRRMIALNNSFKTPIGFFTQIDITIANDDELLTLLA
                     DANFYNLMIGIESVNPEALKDMNKKQNMGISAVEAIKKIQSYGMVVLAHMIIGNESDD
                     MTVFKKTADFVMDADIIFHICHPLAAPPGTKMWYDYKRQGRVIQNEPDETSDKMDIIS
                     NIIPKQMSRQELFLGLADYWDDIYQAEKFKERAVSFIRNINRRANIKMPGFSAIWAMR
                     KMMAGVFRYFFINAEKIHRKTFMYILKSARKNLSYMMPKIVYIYTFYLIDLKRSQYDA
                     MVAREHARWEREHPEKIQIDTSEIPVYEKIREHASALIPASYEYIWKKCNKKEVLYKT
                     VHAALLEFSDRFGKDFEAPDEFYLEQMRIASERVLPSIMEAGFLCADELPEKQPGGFT
                     REILDALDNAVRYRTIYG"
     gene            2209..3231
                     /locus_tag="PLI16_02740"
     CDS             2209..3231
                     /locus_tag="PLI16_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOH83506.1"
                     /translation="MADSTYKVLKIVKIAKRWGLDWFILALVAAIVLAFLWPEAGYKE
                     SVIPLSTIATWGISVIFFFYGLKLNPQKLKSGLKNWRVHLVIQLTTFGFFPLLVIIAK
                     SIIGTAVDENLWLGIFFLACLPGTISSSVVLVSIAKGNMPSAIFNSSISTLLGIFITP
                     FWIGLYLGTSSADFDFGPVIIKLLLQVLFPVILGMLLHFKWGAAAEKYGNILAYFNQI
                     VILLIVYTSFCDSFANNIFAGYSAVMLMLLGASLILLFYIVYFVVFVAGRLLGFSREE
                     KITALFCGSNKSLVHGSLMSKVLFAGSPLAGVVLLPIMMYHALQLVMGSIIAQRFAGN
                     ISGANS"
     gene            3285..4622
                     /gene="purB"
                     /locus_tag="PLI16_02745"
     CDS             3285..4622
                     /gene="purB"
                     /locus_tag="PLI16_02745"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOH83507.1"
                     /translation="MELSPLTAVSPIDGRYHKATETLSGYFSEYALIKKRVFIETEYF
                     IELCAMIPALKKVKEREFETIRDIHRNFDEDDAIKIKEIEKITNHDVKAVEYFLREQF
                     DAMKIGAYKEFIHFGLTSQDINNTAMPLLIKEAQLNAVLPALNKLLDRLHTLAGEWSD
                     IPMLAHTHGQPATPTRIGKEIMVFAERLHGQLMHFDDITFEGKFGGASGNLNAHYVAY
                     SDKDWHGFAEKFLSRLGLKRQRYTTQIEHYDNLAAYFDTLKRICTLLIDLCRDIWLYV
                     SMDYFKQKIVKGEVGSSAMPHKVNPIDFENAEGNLGLAIALFEHLSAKLPVSRLQRDL
                     TDSTVTRNIGVPVAHLMIALKSIDKGLGKLIVNEKKITADLDQNWAVVAEAIQTILRR
                     ENYSDPYNALKDLTRNNQVITKEILHDFIDTLTVSKKVKEELKYINPRNFSGK"
     gene            4716..5771
                     /locus_tag="PLI16_02750"
     CDS             4716..5771
                     /locus_tag="PLI16_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOH83508.1"
                     /translation="MKKILFSVVFFITGVWAVAQIPPGYYDSAYYLSGESLRVALHNI
                     IKNHTAISYTQLWNAFQNTDKKPNNKVWDIYSDIPGGTPPYEFTFVSDQCGSYSSEGD
                     CYNREHSFPQNWFDDSSPVYSDLFHMYPTDGYVNNKRGNLPFGDVGSASWTSQNGSKI
                     GNCSNAGYSNTVFEPIDAFKGDLARSYFYIAVRYYGEAGSWPGSDMTINAEPKPWAIN
                     MLLAWHANDTVSQKEIDRNNAIYNIQHNRNPFIDHPDWVWNIWGPNVGVQEYADVFEV
                     SVYPNPATDYVYVSSISGQIVDRIEIYSLTSALVGSFQYPGEGQSINVSNLPEGFYTM
                     AIHSNGRVSHNKISIIH"
     gene            complement(5791..6783)
                     /locus_tag="PLI16_02755"
     CDS             complement(5791..6783)
                     /locus_tag="PLI16_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOH83509.1"
                     /translation="MKISKEVKIAVVFIVGLALLYWGINFLKGRDLFKKERTFYAVYN
                     QVDGLVKANPVLVNGYRVGQVDDIRFHPDNSGRIIVKIKVTNNDLMIPANTEARIISS
                     DILGSRAVELKRGNATTEAKDGDTLSSSLQATLTQEVNVQFQPVKQKFENVMLSLDSV
                     LVIIKSVFNENTRQNLEQSFESIRFTIRNLEHTTYNIDTLVTNQRSNLAGIIANVESI
                     SENIKNNNDKIQNIIKNFSDISDTIAQSNIASTINNADKSLKSFSEIVDKVNRGEGTL
                     GMLIYNDSLYNNLEKSSKELNELVEDIKLNPHRYLNFSVFGPSKKKNEYTPPKE"
     gene            complement(6814..7977)
                     /locus_tag="PLI16_02760"
     CDS             complement(6814..7977)
                     /locus_tag="PLI16_02760"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH83510.1"
                     /translation="MKRNNLIFVLLALMILGIPGYVTAQAIKIRKVVIDAGHGGDDPG
                     AVGKKAREKDITLSVALKIGNYIKTAFPDIEIVYTRKTDVFVELYKRAKIANDHKADL
                     FISIHCNASKSPEPVGTESWVMGLHKSQANLEVAKKENAAILYENDYSVRYDGFDPNS
                     PEANIIFSLYQNAYLDQSLTFSSFVQNQFTSKAGRFSRGVKQAGFLVLYKTTMPGALI
                     EIGFISNPTEELYLMSESGQNIIASAIYRAFKEYKANVEGVAGKQEVKTEPDTIFQKI
                     TVSDTSPVVNNTDTLSRINETKDNSPYFRVQFLSSSRKKSLDVPEFKGLDNVRYYVQN
                     GIYKYTCGNFNTLKEAITYQHVVQNKGFKDAFVVAFLNEERISPTEALKYSGN"
     gene            8134..>8485
                     /locus_tag="PLI16_02765"
     CDS             8134..>8485
                     /locus_tag="PLI16_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83511.1"
                     /translation="MMFLFFTVQAQDPVSNITTDSARIVSDTIKNDSIIDLRKQESPD
                     AIKTKIDYKAKDSIIMNLDERKSYLYREVKIDYEDITLKSGYVEIDFQKNQLFAEGLK
                     DSAGNEIERPEYEQG"
BASE COUNT         2320 a         1802 c         1918 g         2445 t
ORIGIN      
        1 gttcattaaa tgttattttt gtgcgcagca taattacctc tcaatgtgac ctgaaatttt
       61 gttattattt gttggttgta taagcaacat aattacctct cagcataact tgtatttatg
      121 cattgccata cttttgtaat aaaaaattca aaacagtttc ttacttttga taactttttt
      181 caacgacaca attacggttg cattgtgcaa tgtattaacg gacatagctt ttaaaaccca
      241 aaccctcaat aattgcattg ataatgaaaa ttttacttat aagcccggga acaccagatg
      301 agattgataa taaaattatc cgcagcatac cctatctgaa tgcgaaagct tttattgctc
      361 ctcacgccgt agccacagtg gcggccctta cacccgaaga acatgatgtg gtaatacatg
      421 atgaatacat ccgcgggcct tcggaggatt atttcagagg aaaaagtttt gatattatcg
      481 gcatttccat cacctctaat cagttgaaac gcagtctcga aatagcgcag gcctgtaaaa
      541 agttttgccc ggattcctta gtggttgtcg ggggtattgg agtggaaatg cttatttata
      601 aacagaaaga aaatatagat gtcgtgtttc acggcgaagc ggaagaaaca tggccaaggt
      661 ttttagaaga ctataaaaac aggacacatc agaaagttta taaaagcgtc atcaagcccg
      721 acatgagtaa agtgccgccg ccacgctggg aacttatcaa agaagacata cttctgtata
      781 actccgtttc gctgcagact acaaggggat gccccttcga ctgcagtttt tgtgatgtga
      841 tttataccta tggacgcaaa ccccggagta aaaccattga gcaggttatc gacgaaataa
      901 aaaaacttca cagcctggga gtacagatgg tttttattgc cgacgacaac tttgccggag
      961 acaagaaata tgtcaaagaa ctgttgcggc ggatgattgc gctgaataat tctttcaaaa
     1021 cccctatagg ctttttcacc cagatagata tcaccatagc caatgatgac gaactgctga
     1081 ccttgctggc cgacgccaat ttttacaacc tgatgatcgg catcgaatcg gtgaacccgg
     1141 aagcgctgaa ggacatgaac aaaaagcaga acatggggat ttcggccgtg gaggctatca
     1201 aaaaaattca gtcgtacggg atggtggttt tagcccatat gattatcggc aacgaatcgg
     1261 atgatatgac cgtatttaag aaaacagcgg actttgtaat ggatgccgac attatttttc
     1321 atatctgcca tcctctggcg gcgcccccgg gcaccaagat gtggtacgac tataaacgcc
     1381 agggccgcgt catacagaat gaacccgacg aaaccagcga taaaatggat atcatatcca
     1441 atatcattcc gaagcagatg agccgtcagg agctgtttct ggggcttgcc gactattggg
     1501 acgatattta ccaggccgaa aagttcaaag aaagggcagt gtcgtttatc cgcaacataa
     1561 accgccgcgc gaatattaaa atgcccggat tttcagcgat atgggctatg cgaaagatga
     1621 tggccggcgt attccgctat ttttttatca acgccgaaaa aatacaccgg aagacattta
     1681 tgtatatctt gaagtcggcc agaaaaaacc tcagctacat gatgccgaaa attgtctata
     1741 tttatacttt ttacctgatc gacctgaaac gctcacagta cgatgccatg gtggcccgcg
     1801 aacatgcgcg ctgggaaagg gaacatcctg aaaaaattca aatcgacact tcggaaattc
     1861 ccgtgtacga aaaaatccgt gaacatgctt cggcgcttat ccctgcttca tacgaatata
     1921 tttggaaaaa atgcaacaaa aaggaagtgc tctataaaac tgttcatgcg gcgctgcttg
     1981 aattcagcga ccggtttggg aaagattttg aggcgccgga cgagttttat cttgagcaga
     2041 tgcgcattgc cagcgaaagg gtactgccct cgattatgga agcgggattc ctttgtgctg
     2101 acgaactgcc cgaaaaacag ccgggaggtt ttacgcgcga gatactggat gccctcgaca
     2161 atgcggttcg gtaccgcact atatatggat aaatgttgaa aaatcattat ggctgacagc
     2221 acctacaagg ttttgaaaat agttaaaatt gctaaacggt gggggctcga ctggtttatc
     2281 ctggcattgg tcgccgcgat cgtgctggcc tttttatggc ctgaggcagg atacaaagaa
     2341 tcggtgattc ctctgtcaac cattgctacc tggggtattt cggtaatttt ctttttttac
     2401 ggactgaaac taaaccctca aaaattaaag tcgggtttga aaaactggag ggtacacctg
     2461 gtgattcagt tgaccacttt tggatttttt cccttgctgg tgataatagc taaaagcatt
     2521 attggtacgg cggttgatga aaatttgtgg ctggggattt tctttctggc ctgcctcccc
     2581 ggcactattt catcctcggt ggtcttggtt tcgattgcca aagggaacat gccttcggcc
     2641 atctttaatt ccagcatttc taccctgttg ggtatattta ttaccccttt ctggattggc
     2701 ttgtacctcg gtacgtcatc ggctgatttc gactttggac cggtaataat taaactgttg
     2761 cttcaggttt tgtttccggt aatacttggc atgttgcttc attttaaatg gggcgctgca
     2821 gccgaaaaat acgggaatat tcttgcgtac tttaaccaga tagtaatttt gctgattgtt
     2881 tatacttcct tttgcgactc gtttgctaat aatattttcg cggggtattc tgccgtcatg
     2941 ctgatgcttc tgggcgcctc cctgatactg ttgttttata tagtttattt tgtggtgttt
     3001 gttgccggca gattgttagg gttcagcagg gaagaaaaaa tcaccgcttt gttttgcggt
     3061 tccaacaaat cgttggtgca tggcagcctt atgtcaaaag ttttgtttgc aggcagcccg
     3121 ctggcaggtg ttgtgttgct ccccatcatg atgtatcatg cattgcagtt ggtgatgggc
     3181 agcatcattg cacaacggtt tgcagggaat atttccgggg caaattcatg aatttgcccg
     3241 gctgcctgaa aaaatcatta tctttgttta ataaatcaga tcctatggaa ttaagtccct
     3301 taactgccgt ttcgccgatt gatggccggt atcataaagc taccgaaaca ctttccggat
     3361 atttttctga gtatgcactc atcaaaaaaa gagtatttat tgaaacagag tattttattg
     3421 agttgtgtgc catgataccg gcattgaaga aggtcaaaga gcgggaattt gaaaccatac
     3481 gggatattca cagaaacttt gacgaggacg atgctataaa aatcaaagaa atcgagaaaa
     3541 tcaccaatca cgatgtgaaa gccgtggagt attttttgcg tgaacaattc gatgccatga
     3601 aaattggggc atacaaagag tttatacatt tcgggcttac ctcccaggat atcaataata
     3661 cggccatgcc gctgctcatc aaagaagccc agttgaatgc ggtattgcct gcgctcaaca
     3721 agctgctgga ccgcctgcat accctggcag gggagtggag cgatattccc atgttggccc
     3781 acacccatgg ccagccggcc acgcctaccc ggataggaaa agaaatcatg gtttttgccg
     3841 aacgcctgca cggtcagctc atgcattttg atgatatcac tttcgaagga aagtttggag
     3901 gcgccagcgg caacctcaac gcgcattatg tggcatactc cgacaaagac tggcacggtt
     3961 ttgcagaaaa atttctgtcc cgcctcggcc tgaaacgcca gcgttatacc actcagatag
     4021 agcattacga caacctggcg gcctatttcg acacgctgaa gcggatttgc accctgctga
     4081 tcgatttgtg ccgcgatatt tggctgtatg tttcgatgga ttattttaag caaaaaattg
     4141 tgaaaggcga ggtgggctct tcggccatgc cgcataaggt aaaccctata gattttgaaa
     4201 atgccgaggg taaccttgga ctggccatag cgctgtttga acacttatca gccaagttgc
     4261 ccgtctcgcg cctgcaacgc gaccttacgg attctaccgt tacccgtaac attggcgtgc
     4321 cggtggccca cctgatgatt gcactgaaat ctattgacaa aggcctgggc aaactgatag
     4381 ttaacgaaaa aaaaatcacg gccgatctcg accaaaactg ggctgtagtg gccgaagcca
     4441 tacaaaccat cctgcgcagg gaaaattact ccgacccgta caacgcattg aaagacctta
     4501 ccagaaacaa tcaggtcatc acaaaagaaa tactccacga ttttatcgac acactgaccg
     4561 tgagcaaaaa agtaaaagaa gaactaaaat atatcaaccc cagaaacttt tccggtaaat
     4621 aatgaccaag ggaagtttgt aaagatgaat aaataataat agatgattaa ttatgtgtgt
     4681 ttctgagata taaatactta acccaacaaa atagcatgaa aaaaatatta ttttccgttg
     4741 ttttttttat taccggtgtg tgggctgttg ctcaaattcc tccgggatat tatgattcgg
     4801 cttactacct gtcgggcgaa tcgctgaggg tggcactgca taatatcatc aagaatcaca
     4861 cggccatttc ctatacccaa ttatggaatg cttttcagaa taccgataag aaacccaaca
     4921 acaaggtctg ggatatttat tctgatattc cgggcggaac accaccctat gagtttactt
     4981 ttgtttcgga ccaatgcggc tcttattctt ccgaaggcga ttgctacaac cgcgaacatt
     5041 catttccgca aaactggttt gatgattcat cgcccgttta ttccgatctg tttcacatgt
     5101 atcctaccga tggttatgtc aacaacaaac gggggaattt gccttttggc gatgtgggct
     5161 ctgcgagttg gacatcgcaa aatggaagta aaatcggaaa ttgttccaat gcagggtatt
     5221 caaacacagt ttttgagcct attgatgctt ttaaaggcga cctggcccgt tcctattttt
     5281 atattgccgt gaggtattat ggcgaggcgg gaagctggcc gggcagcgac atgactatta
     5341 acgccgaacc caaaccctgg gccatcaata tgctgctcgc ctggcatgcc aatgatactg
     5401 tgagccagaa agagatcgac cggaacaatg ccatttataa catacagcac aaccgtaacc
     5461 cctttatcga ccatccagac tgggtatgga atatctgggg ccccaatgtg ggggtacagg
     5521 aatatgctga tgtcttcgag gtgtctgtgt atcctaatcc cgccacggat tatgtttatg
     5581 tcagcagtat cagtggacaa attgttgacc ggatagaaat atattcactc acatctgcgc
     5641 tggtcggaag ttttcaatat cccggtgagg ggcaatcaat aaatgtcagt aatcttcccg
     5701 aagggtttta cactatggcg atacacagca atggcagggt atcgcataat aaaatttcta
     5761 taatacatta aacaccagcc tgaaaaactg ttattctttg gggggggtgt attcgttttt
     5821 ctttttactt ggcccgaaaa ccgagaaatt caggtaacga tgcgggttaa gtttgatgtc
     5881 ttcgacgagt tcgttaagtt ctttggaaga tttttcgagg ttattgtaca acgagtcgtt
     5941 gtagattaac atccctaagg tgccttcgcc ccggtttacc ttgtccacaa tttccgaaaa
     6001 tgatttcagc gacttgtcag cattattaat tgtgctggcg atattggatt gtgcaatggt
     6061 atcgctgatg tcagagaagt ttttaatgat attctgaatc ttgtcgttat tgtttttgat
     6121 attttcggag attgattcca cattggcaat aatgccggcc agattgctgc gctggttggt
     6181 taccagcgtg tcgatattat aagtggtatg ttcaaggtta cggatagtga agcgtatgct
     6241 ttcaaaactt tgttccaggt tttgccgggt gttttcattg aacacacttt tgatgatgac
     6301 gagcaccgaa tccagcgaaa gcattacatt ttcgaacttt tgctttaccg gctgaaattg
     6361 cacattcact tcctgcgtga gggtggcttg aagcgaactg ctcagggtgt ccccgtcttt
     6421 tgcttcggtg gtggcattgc cccgtttcag ctcgacggcc cgggaaccaa gtatgtcgga
     6481 actgatgatc cgtgcttcgg tattggccgg tatcatcagg tcgttgttgg taactttgat
     6541 tttaacaata atgcggccgg aattatccgg atgaaaacgg atatcatcca cctgtcccac
     6601 gcggtacccg ttaaccagca ccgggttggc cttaaccagc ccgtccacct ggttgtacac
     6661 ggcataaaaa gtacgttctt ttttgaagag gtccctgcct ttcaaaaaat ttatgcccca
     6721 atacagcagt gctaagccaa caataaagac aacagcgatt tttacttctt tcgagatttt
     6781 caaaacgtat ttttttgcaa atatagttat tttttaattt ccggaatatt tcagcgcctc
     6841 ggtgggcgaa atgcgttctt cgttgagaaa ggccaccaca aaggcgtctt taaatccctt
     6901 attttgaact acatgctgat aggtaatggc ttccttcagg gtgttgaaat ttccacaggt
     6961 atatttatat atcccgtttt gaacataata tcttacattg tcgaggcctt tgaattcggg
     7021 cacatccagt gattttttcc tgctgctcga aagaaattgc accctgaaat aagggctgtt
     7081 atcctttgtt tcattgattc ggctgagggt atcggtatta tttaccacag ggctggtgtc
     7141 gctgacggta attttctgaa aaatggtatc gggttctgtt tttacctcct gtttaccggc
     7201 cacgccttct acgtttgcct tatattcttt gaatgccctg taaatagccg aggcaatgat
     7261 gttttggccg ctttcggaca tgaggtagag ttcctcggtg gggttgctga taaagccaat
     7321 ttcgatcagg gcgccgggca tggtggtttt gtacaaaacc aaaaaaccgg cctgtttaac
     7381 tcccctgctg aatctgccgg ccttgctggt aaactgattc tggacaaaac tcgaaaaggt
     7441 aaggctttgg tcgaggtagg cattctggta cagcgaaaaa ataatgttgg cttcgggcga
     7501 gttgggatca aagccatcgt aacgtacact gtaatcattt tcatacaaaa tggcggcatt
     7561 ttcctttttt gccacttcga ggttggcctg gcttttgtga agccccatca cccaggattc
     7621 cgttcccacg ggctctggac ttttgctggc attacaatgt atcgaaataa aaaggtccgc
     7681 tttatggtcg ttggcaattt tggctctttt atacaactcg acaaaaacat cggtcttgcg
     7741 ggtgtataca atctcgatgt cggggaaagc cgttttgata taattgccaa ttttcagggc
     7801 taccgacaag gtaatatctt tttccctggc ctttttgccc acagctccgg ggtcgtcacc
     7861 accgtgcccg gcatcgataa caaccttcct gatttttatg gcctgagccg ttacgtagcc
     7921 cggtatccct aaaatcataa gggccaacaa aacaaaaatt aaattattcc gtttcatcag
     7981 gatgatttat tttaattagt tttgtcaata atttcaggtt ttttgtgacg caaaattatg
     8041 cgctttttaa ttaacagaat cagcactttg ttcaatttgt caacgaaatt attaaccata
     8101 tttttatttt cccgtttttt tgctgttaca gcaatgatgt ttttgttttt taccgtccag
     8161 gcgcaggatc ctgtaagtaa cataacgacg gacagcgcca ggatagtatc tgatacaatc
     8221 aagaatgatt ccattattga tttgcgcaaa caggaatcgc ccgatgccat aaaaacaaaa
     8281 attgattaca aggccaaaga ctctatcatc atgaatcttg acgaaaggaa atcctatctg
     8341 tacagggagg ttaagattga ttatgaggat attacgctta aatcgggcta tgtggaaata
     8401 gattttcaga aaaaccagct ttttgcagaa ggccttaaag acagtgccgg gaatgaaata
     8461 gaaaggcccg aatatgagca gggcg
//
LOCUS       DAOSFP010000057         8410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7217_length_8410_cov_3.547696, whole genome shotgun sequence.
ACCESSION   DAOSFP010000057 DAOSFP010000000
VERSION     DAOSFP010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7217_length_8410_cov_3.547696"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..966)
                     /locus_tag="PLI16_02770"
                     /pseudo
     CDS             complement(1..966)
                     /locus_tag="PLI16_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
     gene            complement(977..3046)
                     /locus_tag="PLI16_02775"
     CDS             complement(977..3046)
                     /locus_tag="PLI16_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH83512.1"
                     /translation="MKRIFVIISALLIVSATEAKKVDLPLATKTGIAYYYEHAGQFTT
                     VDLKNMTVSDVFTEWDGNVPLYYIINFSSKGFVIVSADDNISPVIGYSYESNLDPAKM
                     PVNLKCFMSEVRQEIKTALAENIKADEYISSQWAYYSTAENLTVKKSKAVVPLLPSSW
                     NQDKYYNQFCPVAEGGPDGKAYAGCVATAMSQVMYYYRYPITGQGTHGGINFGTTTYD
                     WENMLDELSNYNEAVAELMYHCGKAVDMDYAADGSAASSSSCTTALKNYFKYDSNVDF
                     EMKYLYSASGWTSLLKANLDAKHPMIYSGSDGFEGGHAWNCDGYDASDNFHMNWGWGG
                     YANGYYATSNLVAAGSTFNSNQGVVRNIYPPAASYPYNCSGTKSITGVTGTVEDGSGP
                     NDYANNKDCLWLIDPAETVSRIILTFTDFSTQATTDVVTVYDGSTTASPVLGTYSGST
                     LPSAVTSTGPQMLIRFQTNASTTNTGWRASYRCTFPTYCSGITNLTAATGTISDGSAA
                     ENYTYNHLCRWLIAPANASSITLTFTAFNLATNDNVKIYDQNTNNLLDDYTGTSLPGV
                     KTYNTSKVLVLFKSDGYLNAQGFNLNYTSSSQLGIEDNGGPGMMNIYPNPANSFLNVQ
                     FTLDEAQDVNIELCSMTGMAIFREGKSHFSGEYFNTINTSGLSQGVYLLRITGEKLSA
                     CKKVIIE"
     gene            3210..4472
                     /gene="hutI"
                     /locus_tag="PLI16_02780"
     CDS             3210..4472
                     /gene="hutI"
                     /locus_tag="PLI16_02780"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOH83513.1"
                     /translation="MMLVIKNIKELVQVEPNPEKLVMKVSGKKMSELKSIKDAFLIIE
                     KGTISEFGSMKSFDATLYNTGSGNKVLDAGGRMVFPCFCDAHTHLVYAGSREIEYIDK
                     IKGLSYEEIAKRGGGILNSAKKLHNTSEEELIQQALPRLEEIMRLGTGAVEIKSGYGL
                     TTTDELKMLRVIKKLKKLSPLTIRATFLGAHAVPLEYKGRQGEYVDLIINEMIPLVAT
                     DELADYIDVFCDRGFFTVEETERMLMAGIKYGLRPKIHANELDYSGGIQAGVKYNALS
                     VDHLEHTGDDEIQALLGSETMPTLLPGAAFFLGMPYAPARKMINAGLPLAMASDYNPG
                     SSPSGNMQLILSMGCIKYKMLPEEAINAVTINGAYAMGVSDELGTIAVGKKANVFITK
                     PIPSVEFMPYAFGSNKVETVIINGKIQK"
     gene            4469..5161
                     /locus_tag="PLI16_02785"
     CDS             4469..5161
                     /locus_tag="PLI16_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOH83514.1"
                     /translation="MKTIGLLGGTTWESTQDYYRIINQQMLLKAGNRNTAKIVMYSVN
                     FHEVEVLVNAKKYDEVGSLMNKAALAIEKAGADCLLICANTMHMVADGIMAGVHIPLI
                     HIVDETIKEIKRNGYTKVGLLATRQTMEMDFYKERCRKNGVEIVIPGTEDRDFIQATI
                     FNELFNGIISESSRERMLGIMKDMEKLGTQGVILGCTEIPLLIKQEHTDIPVFDSTYI
                     HSCAAVDFALNN"
     gene            5172..7364
                     /locus_tag="PLI16_02790"
     CDS             5172..7364
                     /locus_tag="PLI16_02790"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOH83515.1"
                     /translation="MKQKFKIKKRYIILGSIALLIFLLLFFLSSIVKWYVVKNSEDLI
                     GRKVDIAGLHINYFKWTVKIEDFTMYELNKKDTFVNFQELFVNFDPWNLLHKEIAFSE
                     IRLDKPWVSIVYADSVFNFNDFLAPGDTTAADTIPEEESSDTLKYTVKNFSINGGYIS
                     YEDKSLDTKTELKNLGVKVPEISWNSKQSDLGVEFLLGEGGEVAVDGSLNQAAGKYSV
                     SLKTNNIDITPFAGYTRPFLDANINSGQLYTNLKVDGELENFLNIVVTGEAGIKNLSV
                     TELDNKPFCSFKDIYVRMDSLDIGKSNFCIDKVLLDEPNIVAVLERGNTNIQRILAPI
                     LNDTAASADTTTDTTVMHYSIDSLVIRGGNINFSDLTLNRPFNFDIQNLNVGLTGFSD
                     QATGIPVDFSMNLNGPGTFKGKGTVDMVNTSNIIFKGTIANLNLVTFSPYSEYFLARP
                     ITRGTFNYDCTLKMTPNLLDNLNNLKLAQLEFGKKTKDTSAYKLPVALALYIIKDREG
                     NIKIDLPVSGKPSDPSFKLRKIIWKTLEEFLLKAISEPFEAIGKLFGANPESIKQIPF
                     EYLQDSLTENQRGKLDKIAEIITKKSELSYTFIQTTDPGKEKAMIAVQEAKQLFVAKT
                     VQPGTDPASIKIAASKVVDADPAFLLFVGIEGEDHGNLNSRCMQLVGSEKVEGLFNNL
                     IQKRQDLIKGYLGTKELPQGSVAFKIADFSNMPEEMKSPKAIIELNVK"
     gene            7423..>8410
                     /gene="ftcD"
                     /locus_tag="PLI16_02795"
     CDS             7423..>8410
                     /gene="ftcD"
                     /locus_tag="PLI16_02795"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOH83516.1"
                     /translation="MKQLIECVPNFSEGNNMNIIKQITDVMEAVPGVKLLDVDPGKAT
                     NRTVVTIAGAPEDVVEAAFRGIKKASEIIDMRKHKGEHPRFGATDVCPFVPIAGITME
                     ETVVFAHKLAERVGNELGIPVYCYENAAFTEERRNLANCRAGEYEGLQKKLSDLHWKP
                     DFGPAKFNAHAGATAIGARDFLVAYNINLNTTSTRRANAVAFDIREKGRPKREGNPVT
                     GKIVKDAEGNNVMIPGSLKACKAIGWFIEEYGIAQISINLTNISITPVHVAFEEACIK
                     SGERGLRVTGSEIVGLIPLKAMLDAGKYFLKKQHRSVGVSQDELIKIAIKSLG"
BASE COUNT         2405 a         1846 c         1971 g         2188 t
ORIGIN      
        1 ataatacaat ggctttttgg cgttcagatg gttcaatatc agttgtttcc aattagtatg
       61 cgtagctgta tccctgaaaa tgtattgaca atcgggagca tagccaaaat aagtgtgcag
      121 ggcataagct gctttgtggt tgtacattcc tgagccggaa gaatcgtaat tcagattaac
      181 agaaactcca aggtggtaaa gcagttttgc tacggcggaa tcattctgtc gttcggtaac
      241 agccaatggc atctgatctc agcgatacac cgtattgcca aaatctgctg ccagcgaatc
      301 atacactaaa gggccaacgg tgtcgttgta cacatatgaa cctgtgcctg taacaggatg
      361 ccggtaataa ttcatgatct gtcccatggc tgtgggcaca caacctgcgt aagcacgacc
      421 gtcggggccg ccggcagcag gaggacaaaa attattataa tatttgtcct ggttccaggt
      481 agttttcagc aggggaggaa cggctgtagt ttctttgctc ataattaact gttggggagc
      541 ggctgtgagc agcctgtccc attcagcggc aacggaagta ttttgcaggg gatggttttg
      601 taatacataa agcatctgtt tttcataatc ttccatccac ctgacgaagg caggaggcag
      661 gttttgcata tcataacggc tgtcgaagac ataacacagt accggccgga aaatatcgtt
      721 ggacgaaact accacgaacc ctttattttt aaaggtaatg gtgtaaaaca gaatttggtc
      781 atcaacggtt ctgggaaaga cgctttctgt ttcaagagtt ttataatcta tggaagaaac
      841 aaggcttaac ctttcataaa agaaattttt gccggcgagt atagcctgtt gctggctgat
      901 gttttttgaa aaagcaacac tttggatcag tatgcagcag actatggcaa tgagtttttt
      961 ttgcataaaa gcgctattat tcaataataa cttttttaca tgcagatagc ttttcgccgg
     1021 tgatccttag cagataaacg ccctggctga ggccggaggt attgatggtg ttgaaatact
     1081 ctccggagaa gtggcttttt ccttctctga agattgccat accagtcatc gaacacagtt
     1141 caatattgac atcctgcgct tcgtcaaggg taaactgcac attcaggaag ctgttggcgg
     1201 gattcggata gatattcatc atgcccgggc cgccattgtc ttcgatgccg agttgactgg
     1261 atgaagtgta attaaggtta aaaccctgtg cattgaggta gccatcggat ttaaacagta
     1321 ccagaacctt gctggtattg tatgttttca cacctggcag gcttgtgcct gtataatcat
     1381 ccagcaggtt gttggtattc tgatcataaa tttttacatt gtcgttggtg gcaagattga
     1441 aagccgtgaa cgtcagggtt atggaggatg cgttggccgg agcaatcaac cagcggcaaa
     1501 ggtggttgta tgtatagttt tcagcagcgc tgccgtcgct tatcgttccg gtggcagcag
     1561 ttaaattggt tattccggag caataggtag ggaaagtgca tcggtaggag gctctccagc
     1621 ctgtgttggt ggtgctggca ttggtctgaa agcgaatcag catttgtggg cctgtagatg
     1681 taacagcgga gggcagcgtg ctacccgagt aagtgcctaa taccggcgaa gcggtggtgc
     1741 tgccgtcata aacggtgacc acatcggtgg tagcctgtgt gctgaagtct gtgaaggtca
     1801 ggatgattct tgaaacggtt tctgcaggat caatgagcca caggcaatct ttgttgtttg
     1861 cataatcgtt gggtccgcta ccgtcttcca cagtgcctgt aactcctgta atagatttgg
     1921 tgcccgaaca gttgtaggga taacttgcgg ccggagggta aatattcctg accaccccct
     1981 gattgctgtt aaaagtgctg ccagcagcca ccaggttgct ggtagcatag tatccgttgg
     2041 cgtagccgcc ccagccccag ttcatgtgaa aattatcact ggcatcgtac ccgtcgcagt
     2101 tccatgcatg tccgccttca aaaccatccg agccggaata aatcatgggg tgtttggcgt
     2161 cgaggttggc tttcagcagg ctggtccacc cggaagcaga atagaggtat ttcatctcaa
     2221 agtcaacatt gctgtcgtat ttgaaataat ttttcaatgc cgtggtgcat gaggatgaac
     2281 tggctgccga gccatcggcg gcatagtcca tatcaacggc tttaccgcag tgatacatca
     2341 gttcggctac cgcttcgtta taattgctga gttcatcaag catattttcc cagtcatagg
     2401 ttgttgtgcc gaaatttata cctccgtgag tgccctggcc ggttatgggg taccggtaat
     2461 aatacatgac ctgcgacatg gcagtggcca cgcatcctgc ataagccttg ccatcggggc
     2521 ccccttcggc taccggacaa aactgattgt aatacttatc ctgattccaa ctgctgggca
     2581 gcaaaggtac tacggccttg ctttttttca cagtgaggtt ttcagctgta ctataataag
     2641 cccactggct gcttatgtac tcatccgctt ttatattttc ggctaaggcg gtttttattt
     2701 cctgccgtac ctccgacata aagcatttca ggttgacagg catttttgca ggatcaagat
     2761 tggattcgta ggaatatcct atcaccggac tgatattatc atcggcagat actatgacaa
     2821 agcctttgga tgaaaaattt attatgtaat acaatggaac attaccatcc cattccgtaa
     2881 agacatcgct tacagtcatg ttttttaaat ccactgtggt aaattggccg gcatgttcgt
     2941 aataatatgc aattcccgtt tttgtagcca gcggcaggtc aacttttttt gcctcggtgg
     3001 cactgacaat aagtagtgca gagataataa caaatatacg tttcattctt ctattaaatt
     3061 ttaacaaaaa taataataat tttgcacgta cagaaatgca taaatttgtt attaaagctt
     3121 taacaaataa ttaactacaa tatctgttta taggtacttt ttttgcattt aactgtgttt
     3181 ttttattgtt gaaacataaa actttaagaa tcatgctggt aataaaaaac atcaaagaat
     3241 tggttcaggt ggaaccaaat cctgaaaaat tggttatgaa agtttccgga aaaaagatgt
     3301 cggaattgaa atccataaaa gatgcctttc tgattattga aaaaggaaca atcagcgaat
     3361 ttggctctat gaaaagtttt gatgcaacgc tgtataacac cggctcggga aataaagtgc
     3421 tggatgccgg cggccgcatg gtttttcctt gtttctgcga tgcacacacc cacctggttt
     3481 atgccggcag ccgtgaaatt gaatatattg acaaaatcaa gggtttgtcg tatgaagaga
     3541 tagcaaagcg gggaggaggc attcttaact cggcaaaaaa actgcacaac acttcggaag
     3601 aagaattgat tcaacaggca ctacccaggc tggaagaaat aatgagattg ggtaccggcg
     3661 ctgtggagat taaaagcggc tatgggctca ccacaaccga cgaactgaaa atgctgaggg
     3721 taatcaaaaa actcaaaaaa ttatctcccc tgaccatcag ggccactttt ttgggtgccc
     3781 atgccgtgcc tctcgaatac aaaggcagac aaggcgaata cgtggatctg atcatcaacg
     3841 aaatgatacc gttggtggct accgacgaac tggccgatta tatcgacgtg ttctgcgacc
     3901 gcggtttttt taccgtggag gaaactgaac ggatgctgat ggcaggtatc aaatacggac
     3961 tcaggccaaa aatccatgcc aacgaactgg attactccgg tggcatacag gcaggtgtaa
     4021 aatataacgc cctttcggtg gaccacctgg aacacacagg tgatgatgaa atacaggcgc
     4081 tattgggaag cgaaaccatg cctacactct tgcccggtgc tgcttttttc ctcggtatgc
     4141 catacgcacc cgcccgaaaa atgataaacg caggcctgcc tctggctatg gccagcgatt
     4201 ataaccccgg ctcgtcgcct tcgggaaata tgcagctgat actttcgatg ggttgtataa
     4261 aatacaaaat gttgcccgaa gaagccataa atgccgtaac tatcaacggc gcctatgcca
     4321 tgggtgtttc agatgaactg ggaaccatag ccgtgggcaa aaaagcaaat gtctttatca
     4381 ccaaacccat accctcggtg gaatttatgc cttatgcctt cggcagcaac aaagtggaaa
     4441 cagtaatcat taacggaaaa attcaaaaat gaaaaccata ggacttttag gcggcaccac
     4501 ctgggaatcc acccaggatt attaccgcat catcaaccag caaatgctcc tgaaggcagg
     4561 aaaccgtaac acggcaaaaa tagtgatgta ctcggtgaac tttcatgagg tggaagtgct
     4621 ggtcaatgct aaaaaatatg atgaggtagg ctccctgatg aataaagccg cattggccat
     4681 cgaaaaagcg ggcgccgatt gcctgcttat atgcgccaat acgatgcaca tggtggccga
     4741 tggcataatg gcaggtgtcc acattcccct gattcacatt gtggatgaaa ccattaagga
     4801 aatcaaaaga aatggataca ccaaagtagg tttactggct acccggcaaa ccatggaaat
     4861 ggatttttat aaggaacgtt gccgcaaaaa tggcgtggaa attgtaatac ccggaaccga
     4921 agaccgcgac tttattcagg ccacaatctt caatgaattg tttaatggaa tcatcagtga
     4981 atcatcgcgc gaaaggatgt tgggtatcat gaaagatatg gaaaaacttg gcacacaggg
     5041 cgttattctt ggttgtaccg aaattccgct gttgatcaaa caggaacata cggatatacc
     5101 ggtgttcgat tccacttata ttcattcctg tgcagcggtt gattttgctt tgaataatta
     5161 aatgtgctgt tatgaaacaa aaattcaaaa ttaaaaagag gtacatcata cttggcagca
     5221 tagccctgct gatattttta ctgttgtttt ttctctccag cattgtaaaa tggtacgtgg
     5281 tcaaaaacag cgaggacctt atcggccgca aggtggacat tgccggcttg cacatcaatt
     5341 atttcaagtg gaccgtgaag attgaggatt ttaccatgta cgagctgaac aaaaaagaca
     5401 ccttcgtcaa ttttcaagag ttatttgtga atttcgaccc ctggaacctg cttcataaag
     5461 aaattgcatt ttccgaaatc cgccttgaca agccctgggt atctatagtc tatgccgaca
     5521 gcgtttttaa ttttaacgat tttcttgctc cgggggatac cactgccgcg gacactatcc
     5581 ctgaagaaga aagcagcgat accctgaaat acaccgtaaa aaacttcagt attaatggcg
     5641 gttacataag ctacgaagac aaatccctgg acaccaaaac cgagttgaaa aacctggggg
     5701 tgaaggttcc tgagatttcg tggaacagca aacaatccga cctgggcgtt gaatttcttt
     5761 tgggcgaagg cggtgaagtg gccgtggatg gctcactgaa tcaggcagca gggaaatatt
     5821 cagtctcgct gaaaacaaac aacatagata tcacgccctt tgcaggctat accaggccat
     5881 ttcttgatgc gaacatcaat agcggccaat tatacaccaa cctgaaagtg gacggcgaac
     5941 tggaaaattt cctcaacatc gttgttaccg gtgaggccgg catcaaaaac ctgtccgtga
     6001 cagaattgga taataaacca ttttgctcat ttaaagatat ttatgtacgt atggattcgc
     6061 tggatattgg caaatccaac ttctgcatcg ataaagtttt gctggatgaa cccaatatcg
     6121 ttgctgtact cgaaagaggc aacaccaata tacagcggat actggcgcca attctcaacg
     6181 acacggcagc gtctgccgat accacaaccg ataccaccgt gatgcattat tctatcgaca
     6241 gcctggtgat aaggggtggt aacataaatt tctctgacct cacactgaac aggcctttca
     6301 attttgatat ccaaaacctt aacgtcggac tgaccggatt cagcgaccag gcgaccggca
     6361 tcccggttga tttttcgatg aacctcaacg gccccgggac ttttaaagga aaaggcacgg
     6421 tggatatggt gaacacctcc aatattatat tcaaaggaac tattgccaat ctgaatttag
     6481 tgacgttttc tccctattcg gaatatttcc tggcaaggcc gatcacccgg ggcacattta
     6541 attatgactg caccctgaaa atgactccca acctgctcga caacctgaat aaccttaagc
     6601 tggcacaact ggagttcggc aaaaaaacca aggacacctc ggcctataaa ctacctgtgg
     6661 cgctggcttt gtatattatc aaagaccgcg agggtaatat caaaattgac ctgccggtaa
     6721 gtggaaaacc ttccgatcct tcgttcaaac tgcgcaagat tatctggaaa acactggagg
     6781 aatttttgtt gaaagccatt tccgaacctt ttgaagccat aggcaaactc tttggtgcca
     6841 accctgagag catcaagcag ataccttttg aatacctgca ggacagcctt accgaaaatc
     6901 aacgtggcaa gctcgataaa atagcggaaa tcattaccaa aaagtcggaa ttgagctata
     6961 cttttatcca aaccaccgat cccggcaaag aaaaagctat gatagccgtg caggaagcca
     7021 aacagttgtt tgttgcgaaa accgttcagc ccggcaccga tcctgcatcc atcaaaattg
     7081 ctgcatccaa agtggtggat gccgaccctg cctttttatt atttgtgggt attgaaggcg
     7141 aagatcatgg gaatctgaac agccgttgta tgcaactcgt tggcagcgag aaagtggaag
     7201 gattatttaa taacctgatt cagaaacgtc aggacctgat aaaaggctac ctgggcacca
     7261 aagagctgcc acaggggtct gttgctttta agattgcgga tttcagcaat atgcccgaag
     7321 aaatgaaatc cccgaaagct atcatcgaac tgaatgtgaa ataaatcatt aggtggtcgt
     7381 tattaattag taattttgcg gcctgtaaaa ttcaaaactg ctatgaaaca acttattgaa
     7441 tgtgttccca acttcagcga ggggaacaat atgaacatca ttaaacagat aaccgatgtg
     7501 atggaagccg tgccgggtgt aaaacttctc gatgtggatc caggaaaggc caccaaccgc
     7561 accgtggtta cgattgccgg agcccccgaa gatgtggtgg aagccgcttt caggggaata
     7621 aaaaaagctt cagagatcat tgatatgcgc aagcataaag gcgaacaccc ccgttttggc
     7681 gccaccgatg tgtgcccctt tgtacccatt gctggcatta ctatggaaga aaccgtggtt
     7741 tttgcacaca aacttgccga acgggttggc aacgaacttg gcatccctgt gtattgttac
     7801 gaaaatgctg cttttaccga agaacggcgc aatctggcca actgtcgcgc cggtgaatat
     7861 gaaggtttgc agaaaaaact ttccgacctc cactggaaac cggatttcgg ccctgccaaa
     7921 ttcaatgcac atgccggagc cactgctatc ggcgcccgtg attttctggt ggcatacaac
     7981 ataaatctga atacaacatc cacacgacgc gctaacgccg tggcttttga tattcgtgaa
     8041 aaaggccgcc caaaacgtga aggcaaccct gttaccggta aaattgttaa ggatgccgaa
     8101 ggcaacaatg tcatgattcc cggttcgctc aaagcctgca aagccatcgg ctggtttatc
     8161 gaagagtacg gcattgccca gatatccatc aacctgacca atatcagcat cactccggtt
     8221 catgtggctt ttgaagaagc ctgcataaaa tccggagaac gcggactgcg ggttaccgga
     8281 tcggaaattg tcgggctgat acccctgaaa gccatgctgg atgccgggaa atatttcctg
     8341 aaaaagcaac atcggtcggt aggtgtttca caggatgaac tgataaaaat tgccataaaa
     8401 tcgctgggcc
//
LOCUS       DAOSFP010000058         8333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7317_length_8333_cov_4.036965, whole genome shotgun sequence.
ACCESSION   DAOSFP010000058 DAOSFP010000000
VERSION     DAOSFP010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7317_length_8333_cov_4.036965"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1329
                     /gene="dprA"
                     /locus_tag="PLI16_02800"
     CDS             232..1329
                     /gene="dprA"
                     /locus_tag="PLI16_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOH83517.1"
                     /translation="MLLYQIAVTLIPGIGDITGKKLIAYCGGLEGVFSEKKQHLMKIP
                     GIREVLASSIINGRASALQRAEKEILFIEKYDIQCFFFTEDAYPRRLKNCVDSPMMLY
                     HKGHTDLNNPKIISIVGTRSATEYGKDCCKKIVEGLAGSEVLIVSGLAHGIDTCAHKE
                     AVKNNMGTIGVLAHGLDRIYPAVNRNLALKMLEKGGLLTDYISETNPERENFPKRNRI
                     IAGLSDATLVIEAAKKGGALITADIANSYNRDVFAVPGKITDQYSEGCNHFIKTNKAA
                     LIQSADDIKYLMGWDTKKQKSPPVQRRLFVNLTPDEDAIVKLLELHTQLSVDALCIEL
                     QTTTSLVAAALLNLEFEGIVKNLPGKMYTLA"
     gene            complement(1980..2744)
                     /locus_tag="PLI16_02805"
     CDS             complement(1980..2744)
                     /locus_tag="PLI16_02805"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOH83518.1"
                     /translation="MILLYAEMSIVSLILIAVPLAIAPGVGLCLFIYFRDKFEKEPFR
                     LLKNSFLLGALSIVPAIIIEVLITKLGIDDNQGFLITMIYAFVVVGVTEEGCKYFVLR
                     KYIFPKPEFNEPFDGIVYSMMISMGFATVENIFYVIGSGIGTAVLRMFTAVPLHAVCA
                     VILGYYAGRAKCSGSMFRNIILGFSLAVFVHGLYDFFLFQKNIPALGILSFVALGVSI
                     GLSFVAINKHQKNSPFRKNLAGNVVVEERDNPAENS"
     gene            2865..3482
                     /locus_tag="PLI16_02810"
     CDS             2865..3482
                     /locus_tag="PLI16_02810"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOH83519.1"
                     /translation="MHIAIAGNIGSGKTTLTGILAKHFKWEAHYEDVDTNPYLNSFYE
                     DMQRWSFNLQVYFLNSRFRQVLKIRESGKTVIQDRTIYEDAYIFAPNLHAMGLMNTRD
                     FDNYSSLFELMAAFLKPPDLLVYLRAEVPTLVRQIQKRGRDYENSIRIDYLTRLNERY
                     EAWISSYNIGKLLIVDVDDKDFANNSSDLEKFIDNINGELYGLFK"
     gene            3489..4238
                     /gene="kdsB"
                     /locus_tag="PLI16_02815"
     CDS             3489..4238
                     /gene="kdsB"
                     /locus_tag="PLI16_02815"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOH83520.1"
                     /translation="MHIIGIIPARYASTRFPGKPLVTINGKTMIRRVYEQAAKSQALA
                     TVYVATDDQRIFDHVLEFGGQAMMTSDTHRTGTERCHEVVEKLEAANKFFDVAINIQG
                     DEPYIQPGQIDLLASCFTNPVIEIATLIKKLNSGEELNSPNTIKVVTANNGQALYFSR
                     APIPFVRGKENTQWLQFNTFYKHIGIYAYRTVTLKAITALQPCPLETAESLEQLRWLE
                     NGYHIFTRETCYESYSVDTPEDLLKFDNFAD"
     gene            4295..5386
                     /locus_tag="PLI16_02820"
     CDS             4295..5386
                     /locus_tag="PLI16_02820"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOH83521.1"
                     /translation="MKVLQYISELLLQHDCVIVPGFGGFIATHVPAHIQPVINNFSPP
                     KKDILFNSALKHNDGLLADYIAQKEKISFAEALDMIESFVEQSLQELKHHGRIEMLHV
                     GTIYRDDNNFYTFQQDAEQNMLLSSFGLNTFISPAIQREGYEKRVEKVLAGRKIPQAD
                     RQSTGVWAKVAMISVPAAAIFVWGFINLGPLKEASDNYSNIASIFSGNSNKSTATARQ
                     VNKTNYIIADVYENTLDVEHSKSIFKPEQKTLYPVFTDFVSEASLLVVSKSTISSDTE
                     PAIDTPDAPAMLCKFYIIGSCNRVKDLAENYKTRLISKGYANANILEPKGNGLYKVYI
                     DCYDSEEAANEGLSAIQTNENPDAWLLKM"
     gene            5394..6074
                     /gene="trmB"
                     /locus_tag="PLI16_02825"
     CDS             5394..6074
                     /gene="trmB"
                     /locus_tag="PLI16_02825"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOH83522.1"
                     /translation="MGKNKLERFAETGTFKNFVQLPYYNLSEDFQLKNNWSSGFFGND
                     HPLFLELGCGKGEYTIGLSRMYPENNYIGIDIKGARMWRGAKTALEENLSNAGFLRIH
                     INQINHFFGENEISGIWITFPDPQPQKARANKRLTSPRFLERYRQFLKKDAVINLKTD
                     NVGLFEYTLEVIEQQKLPLLTHTFDLYTSAIDSEASKIKTFYESRFLEEGKKICYLQF
                     CPNQLEKN"
     gene            6061..6426
                     /locus_tag="PLI16_02830"
     CDS             6061..6426
                     /locus_tag="PLI16_02830"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HOH83523.1"
                     /translation="MKKINEDTSFFAMVYEVVRHIPYGRVTSYGAIAEYLGSKGSARM
                     VGWAMNQLHHQKPHVPAHRVVNRNGVLTGKHYFGGTTLMQQLLENEGIVVIDDQIQDF
                     EKLFWDPVKERLFKKSSDD"
     gene            complement(6716..7606)
                     /locus_tag="PLI16_02835"
     CDS             complement(6716..7606)
                     /locus_tag="PLI16_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83524.1"
                     /translation="MENSDQTLKNGSPTANNPVGSTSNASSVQNNDSAQKTNTGGNST
                     NKPSDPKTSSTKTEPLNTSNCKWGTIITAGWIVLVTIVLFVLYEIVNKQLDEKNNVCF
                     AVYWNNSDSIRINPGPSWFLYDQKTDTLRSIIIIDDTSKSLLLGLVKDTTTTGPVDSL
                     KETSNDSYYRAIEALAFKSNSEKRSSYYLLIAIFAVCGAFGSQLRMIYRFIGVTCYKP
                     IFNIKIWWPWYFLLPLLGAVIGPILFMLVEGNLLNYSTTANYSNFLMALTIIAGFAAD
                     DFVRKLNQVSEAFWGHSNTN"
     gene            8130..>8333
                     /locus_tag="PLI16_02840"
     CDS             8130..>8333
                     /locus_tag="PLI16_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83525.1"
                     /translation="MNKLFLFIFCYYPLFLSAQSGNVTYFQNSHKPANSGIARSDMFV
                     WSIHLFGNNFYDNSGKIKENKKNN"
BASE COUNT         2607 a         1775 c         1602 g         2349 t
ORIGIN      
        1 agaagccatc ctaatttggc ttggcttctt tctttttttt atcggacaac agtgacaata
       61 aatcattatc cgcggcttta ctattgttaa cactaatggc aactatttaa gatacaatgt
      121 tttagttatt attgtttttt tggaatttct gctctttttt caaaaaaata tatcttttct
      181 gattatttta ttagttttat gcactttttt caacattgca aaacaaaaaa tatgctatta
      241 tatcagatag cggtaacctt aataccaggc atcggcgata ttacgggcaa aaagctgata
      301 gcctactgtg gcggattgga aggggttttc agcgagaaga aacaacattt gatgaagata
      361 ccgggaattc gcgaagtctt agcctcatca ataattaatg gcagggccag cgcattgcaa
      421 cgtgccgaaa aagagattct tttcatagag aagtatgata ttcagtgttt tttctttaca
      481 gaagatgctt atccacgccg cttaaaaaat tgtgttgact cgcccatgat gctttatcat
      541 aagggccata ccgatttaaa caatcccaaa attatcagca ttgttggtac gcgcagcgcc
      601 accgaatacg gcaaagattg ttgtaaaaaa attgttgaag gcctggccgg cagcgaggtg
      661 cttattgtca gcggcctggc tcatggcatt gacacctgcg cacataaaga agccgtaaaa
      721 aataatatgg gcaccattgg tgtgctggcc cacggactcg acagaatcta ccccgccgtg
      781 aacagaaacc tggcattgaa gatgctggaa aaaggaggcc tgctcacgga ttatatcagc
      841 gaaaccaatc ccgagaggga aaactttccc aaaagaaacc ggattatcgc agggctttcc
      901 gacgccaccc tggtgattga agccgcaaaa aaaggcggcg ccctgattac tgccgacatt
      961 gccaactcat acaaccgcga tgtttttgct gtgccgggca aaattacaga tcaatattca
     1021 gaagggtgta accattttat taaaaccaat aaggccgccc tcatacaatc ggcagatgat
     1081 atcaagtacc tgatgggctg ggatactaaa aaacaaaaaa gtccgcctgt tcaacgaagg
     1141 ctttttgtaa acctaactcc cgacgaagat gccattgtaa aacttcttga actccacacc
     1201 cagttaagcg tggatgccct atgcatcgaa ttgcagacca ccacaagcct ggtagctgcg
     1261 gctttgctga acttagagtt tgaaggaatc gttaaaaacc tgcctggaaa aatgtacact
     1321 cttgcttaag gacacctcag aaaaattcaa aagtatttgc cggattaccg gattttcttt
     1381 tgtcggaccg aagggtgata ttttcgtatg aatgttcagt ttacagcttc ccggtcaacg
     1441 ttatcagagg cgaatttaat gcacaaagac attgaagtgc gtcttgcggc cacgtgccta
     1501 ttttgtttat cccctacggg gaattattgc catagttgat ggtgttacaa ttctttaacc
     1561 tctacgcggt aaataataag atgcccataa atctcccctt attaatcatg cctgtagggc
     1621 atgtagtatt gtaaaaaaaa ttaccccttt tttttcctta ccccgtaggg gttatactcc
     1681 gatgacaact cttcctccct taatatcatt aatgggcacc tttataactt tcacattctt
     1741 cgtttgtgct cgttcttcaa atactcttat acgctcatta tactgaggtt ttcaaaactt
     1801 cgatcgtctc aacctttttt gaagtgcaag gcgattaact ttgcctgtgg caagcgtgga
     1861 ttttcaattt ttgtaatgat tgccggatta ccggattttc ttttgtcggc ccgaagggtg
     1921 aaaaattcgt ataaattttt caggttcgtt ctgccctttc aacattatca gatacgcacc
     1981 taactgtttt ccgcaggatt gtcacgctcc tcaacaacta cgttcccggc taaatttttc
     2041 ctgaaaggag aatttttctg atgcttattg atggctacaa acgacaggcc gatagaaacc
     2101 cctagcgcca caaaagacaa tatccccagc gcgggtatgt ttttctgaaa aagaaaaaaa
     2161 tcataaagac catgaacaaa tacggcaagg gaaaaaccca aaataatatt gcggaacata
     2221 ctgcctgagc attttgccct acctgcatag tatcccaaaa tgacggcaca tacagcatgc
     2281 aggggaaccg ctgtaaacat acgcaatacc gcggtaccaa tgcccgaacc aatcacataa
     2341 aaaatatttt cgactgttgc aaatcccatg gaaatcatca tggaatacac tatgccgtcg
     2401 aaaggttcgt taaactcagg tttgggaaaa atatactttc tcagtacaaa atatttgcag
     2461 ccttcctcgg taacgcccac caccacaaaa gcataaatca tcgtaatcag aaaaccctgg
     2521 ttatcatcta ttcccagttt ggtgatgagc acctctatta taatggccgg aacaatactc
     2581 agcgcaccca gtaaaaaact gttttttaac aaacggaagg gttctttttc aaatttatcc
     2641 ctgaaataaa tgaacaggca caggccaacg cctggcgcta tagccagcgg aacagcaatt
     2701 aatatgaggg atactatact catttcggca tacaaaagta tcaaacttca tgaaaaatat
     2761 ttttttattt tttatctcct ttatgaaaaa aaaactactt ttattcattg aatttttttg
     2821 aataatttac ctgttttaat aacacttttt aacttcaatt tatcatgcat attgctattg
     2881 ccggaaatat cggatcggga aaaacaacgc tgacaggcat attggccaaa cattttaaat
     2941 gggaagccca ttacgaagat gttgacacca atccgtacct gaacagtttt tatgaagaca
     3001 tgcagcgttg gtcgttcaac cttcaggtat attttttgaa tagccggttc cgtcaggttc
     3061 ttaagatccg tgaaagcgga aaaacggtaa tacaggacag gaccatttac gaagatgctt
     3121 acatctttgc gcccaacctg catgctatgg ggctgatgaa cacacgggat tttgataatt
     3181 acagttccct ttttgaactt atggccgcct ttctgaaacc acccgatttg ctggtatatc
     3241 tcagagccga agtgcctacc ctggtacggc agattcaaaa gcggggccgc gactatgaaa
     3301 actccatacg catcgattat cttacccggc ttaacgaacg ctacgaagcc tggatatcgt
     3361 catacaatat cgggaaattg ctcattgtgg atgttgatga taaagatttt gccaacaact
     3421 cttccgacct cgaaaaattt atcgacaaca tcaacggcga actctacgga ttgtttaagt
     3481 agttggtgat gcacatcatc ggtatcatcc ctgcacgtta cgcctccact cgttttccgg
     3541 ggaaacctct ggtaacgatc aacgggaaga ccatgatcag gagggtgtat gaacaagccg
     3601 caaaatcgca ggccctggca actgtttacg tggctaccga cgaccagcga atcttcgacc
     3661 atgtacttga gtttggcggt caggccatga tgacctccga cacccaccgt acgggcaccg
     3721 aacgttgcca tgaagtggtt gaaaagctgg aggcagcaaa caaatttttt gatgtagcca
     3781 ttaatatcca gggagatgaa ccttatatac aacccggcca gatagacctg ctggcttcgt
     3841 gttttaccaa tcccgtgatt gaaattgcca ccctgataaa aaaattaaat tcgggggaag
     3901 aactcaacag cccaaacacc atcaaagtgg ttacggccaa caacggacag gccctgtatt
     3961 tttcacgggc gcccatacct tttgtcagag gaaaagaaaa tactcaatgg ctgcaattca
     4021 acacattcta taaacacatc ggaatttatg cctaccgcac tgtaacctta aaggccatta
     4081 ctgcgttaca accttgtccg ctggaaacgg ccgaatcgct cgaacaactg cgctggctcg
     4141 aaaatggcta ccacattttt acccgcgaaa cctgttacga aagctattcc gttgacacac
     4201 cggaggactt gttaaaattt gataatttcg ccgattgatg atgttttttt tgtaatttag
     4261 catcaccgat tggcagcttc tgaaagttaa gtaaatgaaa gtacttcaat acatcagcga
     4321 acttttgctt cagcacgatt gcgtgattgt tcccggcttt ggcgggttta ttgccaccca
     4381 tgtgcctgca catatccagc cggtgatcaa caacttttct ccccccaaaa aagacatatt
     4441 atttaattcg gcccttaaac ataacgacgg cctgctggca gattatattg cccaaaaaga
     4501 aaagatttca tttgccgaag cgctagacat gattgaaagt tttgtggaac aaagcctgca
     4561 agaattaaaa catcatggca ggatagaaat gcttcatgtg ggaaccattt atcgggatga
     4621 caacaacttc tacacctttc agcaggatgc cgaacaaaat atgctgctgt cgtcatttgg
     4681 cctgaacacc tttatttcgc cagccattca gcgtgagggt tatgaaaaac gtgttgaaaa
     4741 agtgcttgca ggaagaaaaa tcccacaagc cgatcgccag tcaacaggcg tgtgggccaa
     4801 agtagccatg atatcagtgc cagcagctgc catttttgtt tggggattta taaatctggg
     4861 gcctctgaaa gaagcttcgg ataactattc caatattgct tccatttttt cgggtaacag
     4921 caataaaagt acagcaacgg caagacaggt aaacaaaaca aactacatca ttgctgatgt
     4981 atatgaaaac acccttgatg tagagcacag caaaagtatt tttaagcctg aacaaaaaac
     5041 cctttatcct gtttttactg attttgtttc agaagcatcc ctactggtgg tatcaaaatc
     5101 aaccatatcg tccgacacgg aacctgccat cgatacacct gacgcccctg caatgctttg
     5161 caaattctat atcatcggaa gttgtaaccg cgtaaaagat cttgccgaaa attataaaac
     5221 acggttgata agcaagggtt atgctaatgc aaatatcctg gagccaaaag gcaacggact
     5281 gtacaaagtt tatatcgact gttacgacag cgaagaagct gccaatgaag ggttgtcggc
     5341 catacaaacc aatgaaaacc ctgacgcctg gctgttaaaa atgtagattt tttgtgggaa
     5401 agaataagtt agaacgattt gccgaaaccg gtacttttaa aaatttcgta cagctaccgt
     5461 attacaacct ttcggaagac tttcaattaa aaaacaattg gtcttccggt ttttttggca
     5521 acgatcatcc attattcctc gagttgggtt gcggcaaagg cgaatacacc ataggcttat
     5581 cgcgcatgta tcccgaaaat aactatatcg gtatcgatat caaaggagcc cggatgtgga
     5641 ggggcgccaa aactgccctg gaagaaaacc tgtccaacgc cggttttttg cgcatacata
     5701 tcaaccagat caaccatttt tttggagaaa acgaaatcag cggtatctgg ataacttttc
     5761 ccgacccgca accccaaaaa gcaagagcca acaaacgcct gaccagcccg cgcttcttag
     5821 aaagatacag gcagtttctg aaaaaagatg cggtaatcaa cctgaaaacc gacaatgttg
     5881 gcttatttga gtacacgctg gaagtcatcg aacaacaaaa actcccgctg cttacacaca
     5941 cctttgattt gtatacttcg gctatcgata gcgaagcaag taagataaag actttttacg
     6001 aatcacggtt tctggaagaa ggaaaaaaaa tttgttacct gcaattttgc ccgaaccaac
     6061 ttgaaaaaaa ttaacgaaga cacttcattt tttgccatgg tatatgaagt agtccgtcat
     6121 attccatacg gacgggtaac atcgtatggc gccatcgccg aatacctggg cagcaagggt
     6181 tcggcgcgta tggtgggctg ggccatgaac caattacacc atcagaaacc ccatgtgccg
     6241 gcccatcggg tggttaaccg caatggggta cttaccggca aacattattt tggcggcacc
     6301 acattgatgc agcagcttct ggaaaatgaa ggaatcgtag tgattgatga ccagatacag
     6361 gattttgaaa aattgttctg ggatccggtg aaagagcgtt tgtttaaaaa gtcatcagac
     6421 gactaaacgc aacaaaatca ggcaataata ctaagtttag cagtcatcag atgactgcca
     6481 aaagttccat acaattcaaa tggaattttt acctttacac gtgaccaaat aatattattt
     6541 ctctttattc gaaggatcgt catttgttcc tttgccggca acaatgttac tattttattc
     6601 attgccggaa acagattttt accgattgca catatgtaat aagagtccaa cgaattggcc
     6661 tataacatct gttgcatcgg cattatacca taaagcccgt gcatattttt aatgtctaat
     6721 tggtattact atgaccccag aaagcttccg aaacctggtt tagttttcga acaaaatcat
     6781 ctgccgcaaa tccggcaata atagtcaatg ccattaaaaa atttgaataa tttgccgttg
     6841 tcgaataatt taaaagattc ccttctacta acataaataa tattggccca ataacagctc
     6901 ccagcaatgg cagcagaaaa taccacggcc accagatttt aatattaaaa attggcttat
     6961 aacaggttac accgataaaa cggtatatca ttcgcaactg tgatccaaaa gcaccacaaa
     7021 cagcaaaaat cgcgattaac aaataataag aagaccgttt ttccgaattc gatttaaaag
     7081 ccaatgcttc tatggcccga taatatgaat cattacttgt ctcctttaaa gaatcaacgg
     7141 ggccagtagt ggtggtatct ttaactaacc ctaataacaa agacttactt gtatcatcaa
     7201 tgattattat ggatcggaga gtatctgttt tctgatcata cagaaaccat gagggtccgg
     7261 gatttatcct tattgagtca gaattattcc aataaacagc aaaacagaca ttattttttt
     7321 catcaagctg tttgtttact atttcatata aaacaaataa tactatcgtt actaacacaa
     7381 tccatccggc agtaattatc gtaccccatt tacagttcga ggtatttaag ggttctgttt
     7441 tagtagaaga agtttttgga tcagaaggct tattagtgct atttccgccg gtattggttt
     7501 tttgtgccga atcattgttt tgcacagagg aggcattgct tgtactgcca acaggattat
     7561 tagcagtagg tgatccattc tttaatgttt ggtctgaatt ttccatattt atttattttt
     7621 tcatataatc actgattgat attttaaaga tgtaaaatta ttaaattatt attaacaaac
     7681 aaaatttctg gtattttttc aacaaaccga ttatacaaaa ggatattgcg aacaaaccat
     7741 gtttgctgaa gatatcatga caaaggttta ttacaaattc aaacgttggt catgctgata
     7801 gaaaagcata cctcaaaaag gacgaacaaa caacagaaat gattctgaaa aacgttcaat
     7861 agaactattt tttcaaaata gtattatttg attcccgtcg gcccgcaatg aacgccttgt
     7921 aagtgttcgt gcatggattg taatagaaac aagcaacatg aattgttgat tgaaaatttg
     7981 ttacttttgc aagcaatatc aattacacat ttgtaataat ccaacgaatt ggcctataac
     8041 atctgttgca tcggcattat acaataaagc ccgtacattt taaatttcta attggtataa
     8101 aatgcgtctc cgcaaaaaaa atcaaacaaa tgaataaact ttttctattc attttttgtt
     8161 attatccgtt atttttatct gcacagtccg ggaatgtaac ctatttccaa aactctcata
     8221 aacctgctaa ttcaggtatt gcgcgcagcg atatgtttgt ttggagtatt cacttatttg
     8281 gaaacaattt ttatgacaat tccggtaaga taaaagaaaa taaaaagaat aat
//
LOCUS       DAOSFP010000059         8306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7350_length_8306_cov_4.590716, whole genome shotgun sequence.
ACCESSION   DAOSFP010000059 DAOSFP010000000
VERSION     DAOSFP010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7350_length_8306_cov_4.590716"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="PLI16_02845"
     CDS             <1..494
                     /locus_tag="PLI16_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOH83526.1"
                     /translation="NKALLHYYYRSKDKLFDAIFAEAFMKFLPNISQIMMSDECFEKK
                     IRLVISHYTDMLMENPHLPLFIMHEIQRKPDMIVKILRSSGINPAVVAVQLQKEAKEG
                     KIRDIALPHLMTNIIGMCILPFIGRPILEGLLFNGNKKAYDHFLQQRKNLVADFIINA
                     VSK"
     gene            507..1775
                     /locus_tag="PLI16_02850"
     CDS             507..1775
                     /locus_tag="PLI16_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH83527.1"
                     /translation="MKKILLLFVVFPWMSLFAQQTDTISLLYCQQLALDNYPLIRQKE
                     LLSEAAGLKIKNLSTNYFPQIALNGQATYQSAVTEIPQISPMFDFPSMSKDQYKVTLD
                     VNQMVWDGGITSAQKKLETSGLQVEQMSVDVEIAKIKEQVNLIYFNILLAQQNEKVVG
                     NIQDELQSKLKKIEAGIKNEVLLQSNADVLKAEIIKTEQQLIELANAKSAALNMLAEF
                     INRPLYETTELKIPDDIAIVPVFDNKRLEMQLLDLQMNKLDISKSLVNTKVMPRFFAF
                     GQGGYGRPGFNMLSNDFDFFYIVGAKMTWNISGFFQSGREKRIIDIQKNILNTQKETF
                     DKKLKIASQKDAADITKYKQLISKDDEIIRLRQNITKTASTQLENGVITATEYVTELN
                     AEMSARLNLELHKIQMKIAEINYLNLMGKL"
     gene            1842..2726
                     /locus_tag="PLI16_02855"
     CDS             1842..2726
                     /locus_tag="PLI16_02855"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH83528.1"
                     /translation="MKKYLCIISIALLTACSGRNDKSDAYGNFEADETIISSEVSGKI
                     IQLDLEEGQEIKAGTIVGIIDTSDYQLKKEQLTAQKNAIASKSGNIASQIEVQQQQKN
                     NLLIDKARIEKLYKDGAATKKQVDDINGSISMVDKQIQSIQTQNGSVADEILAIVKQI
                     DQISQTIKKCHLSCPVSGTILNKYIEQNELVIPGKSLYKIADLSSVFLRAYISESQLA
                     NVKLGQKVEVLIDKGEDNTAKYEGTVTWISSTAEFTPKIIQTKEERVNLVYAIKIKVK
                     NDGALKIGMPGEVNFNGK"
     gene            2886..3881
                     /locus_tag="PLI16_02860"
     CDS             2886..3881
                     /locus_tag="PLI16_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH83529.1"
                     /translation="MNNVIVENISKSYKKAGTSGNTPIDKIKNARSRKKGLNAASNEI
                     KALDKVSFRVDSGELFGFIGPDGAGKTSLFRILVSLLLPDSGTATVCGFDVVKDFKKI
                     RNIAGYMPGKFSLYQDLTVEENLTFFATIFGTTIQKNYHLIKDIYCQIEPFKDRRAGA
                     LSGGMKQKLALSCAMIHKPEILFLDEPTTGVDAVSRMEFWEMLHNLKKAGITIIVSTP
                     YMDEASLCDRVALIQEGSILKIGTPEEIITDFQKPVFAIRSENTHQLIRDLEKSPECE
                     SVYPFGQYLHFIPENVNIVIDTLSKTIHESHPEAEVFQVKPDIEDCFINLMKEKK"
     gene            3866..4621
                     /locus_tag="PLI16_02865"
                     /pseudo
     CDS             3866..4621
                     /locus_tag="PLI16_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            4745..5104
                     /locus_tag="PLI16_02870"
     CDS             4745..5104
                     /locus_tag="PLI16_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH83530.1"
                     /translation="MHFFKNLEVWKLSMETIVEIYKATDAFPVKEQFGLISQINRSAV
                     SIAANIAEGCGRNTTKDFNHFLDISLGSSFELETLIIASQNIGYLSEEKSLSTINKIH
                     SIQKMLYALRRSTNKPR"
     gene            5241..6353
                     /locus_tag="PLI16_02875"
     CDS             5241..6353
                     /locus_tag="PLI16_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH83531.1"
                     /translation="MKHFFGFVRKEFHHIFRDRRTLIILFGIPVAQMLLFGYIVTTEV
                     KDARIAILDHSKDEITRKIVDKLSSSGYFIIAQHLTNEQEIGECFRKGTVKEVVVFEQ
                     GFAQKLQKEKTAPVHLIADASDANSANLVVNYTAGIINDFVVKENTSTLIPMQIVTEP
                     RMLYNPELKGVYMFVPGTMAMILVLICALLTSVSIVREKELGTMEVLLVSPLKPLQIV
                     LGKVTPYIFLSFVNVITILLLSNFVFGLPVRGSITLLLAESFLFISLALSIGILISSS
                     TSSQQMAMVASMLGLMLPSILLSGFIFPIDNMPGWIQWICYINPTTYFIIILKNIMLK
                     GLGFISVWKETAILAGMTLFLLMVSTKKFKVRLGGK"
     gene            6364..7485
                     /locus_tag="PLI16_02880"
     CDS             6364..7485
                     /locus_tag="PLI16_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH83532.1"
                     /translation="MRTILFILQKEFIQIRRNRMMLPVIFVVPLLQLLILVNAANMEM
                     KHIKICIVDNDLSSSSRLITGKFSNSKFFTLQCHTFNMEDARRQLENDRADIVLHIPA
                     GFERGLVRENKAKVQLLINAINGTVAGISNAYAAQILAGANREIIMQWYGIPKTQAAS
                     AAINIIPAFWYNPDMNYKIFMVPAILVLLVTLIGMMLATLNIVREKEMGNIEQINVTP
                     IRKIHFITGKLLPFWIIALVMLAIGLLIGKLLYDIPMVGSLPLLFVVAGIYLLAMLGF
                     GLMISNTAATQQQAMFVMFFFILIFIMMSGIFTPTDSMPHWAQTINYLNPVAYFMRII
                     RMILLKGSGFADIYRDFLILLGYGITVIIFAVIRYRKVS"
     gene            7582..7653
                     /locus_tag="PLI16_02885"
     tRNA            7582..7653
                     /locus_tag="PLI16_02885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7614..7616,aa:Met,seq:cat)
BASE COUNT         2630 a         1774 c         1651 g         2251 t
ORIGIN      
        1 tcaacaaggc tttgcttcat tattattacc gcagcaaaga caaactcttt gatgccattt
       61 ttgccgaagc tttcatgaaa tttttgccca acatttcgca aattatgatg tcggacgaat
      121 gttttgagaa aaaaatcaga cttgttattt cgcactacac cgacatgctg atggaaaacc
      181 cgcatctgcc tttgtttatc atgcacgaga tacaaaggaa acccgatatg attgtaaaaa
      241 tcctgcgcag ttcgggcatc aatcccgccg tggttgcagt tcaattacaa aaagaagcaa
      301 aagagggcaa aattcgggac atcgccctgc cccacctgat gacaaacatt atcggaatgt
      361 gcatcttgcc ttttatcgga cgaccaatac ttgagggctt attgtttaac ggaaacaaaa
      421 aggcttacga ccatttttta caacaaagaa aaaatttagt tgctgatttc attattaatg
      481 ctgtttcaaa ataaggaact tatcccatga aaaaaatact gctgttattc gttgtgtttc
      541 cctggatgtc tctttttgcc cagcaaaccg acactatcag tctgctctat tgtcaacagc
      601 tggccctgga caattacccg ctgatcagac aaaaggaact tttatctgaa gctgcaggac
      661 taaaaatcaa aaacctcagt acgaattatt ttccgcaaat cgcgctgaac ggacaagcta
      721 cctatcagtc ggcggttaca gaaattcctc agatcagccc tatgtttgat tttccttcga
      781 tgagcaaaga ccagtacaaa gttacccttg atgtgaacca gatggtctgg gatgggggga
      841 taacctcggc acaaaaaaaa ctggaaacct ccggccttca ggtagaacaa atgagtgtgg
      901 atgtcgaaat cgcgaaaata aaagaacagg taaatctcat ttatttcaac atattactgg
      961 cacaacaaaa tgaaaaggtg gtcggaaata ttcaggatga gctgcaatca aaattaaaaa
     1021 agatcgaagc cggaataaaa aatgaggtac tacttcaaag caatgccgat gttctgaagg
     1081 ctgaaattat aaaaaccgag cagcaactta tcgaactggc aaatgcaaaa agtgccgcac
     1141 tgaatatgtt ggctgaattt atcaaccgcc ccctttatga aactaccgaa ctaaaaatcc
     1201 ctgacgacat tgccatcgta cctgtttttg acaacaaacg cctcgaaatg caactgcttg
     1261 atctgcaaat gaacaagctt gacatctcaa aaagccttgt aaacacgaag gtgatgcccc
     1321 gcttctttgc tttcgggcag ggtggatatg gccgtccggg atttaatatg ttgtcaaacg
     1381 attttgactt tttctatatc gtcggagcaa aaatgacctg gaatatttcc ggatttttcc
     1441 agtcgggcag ggaaaaacgc atcatcgaca ttcagaaaaa tattctgaat acccaaaaag
     1501 agacttttga caaaaaactg aaaattgcct cacaaaaaga tgctgccgac atcacaaaat
     1561 acaaacaact gatcagcaaa gatgatgaga tcatccggct caggcagaac attaccaaaa
     1621 cagcgtcgac acaactggaa aacggtgtaa tcaccgcgac agaatatgtt acggaactta
     1681 atgctgaaat gtcagcgcgg ctcaacctgg aattacataa aattcaaatg aaaatagcag
     1741 aaataaatta tctgaatttg atgggaaaac tatagaattc agaagtagga tttcagaagt
     1801 cagaattaaa aaaatcaaaa aaaaataaaa aacaccatta tatgaaaaaa tacctgtgta
     1861 tcatcagcat tgcattatta accgcctgtt cgggcagaaa cgacaaatcg gatgcctacg
     1921 gtaacttcga agccgacgaa acaatcattt cttctgaggt aagcggaaag attattcagc
     1981 ttgatcttga agaagggcag gaaattaaag caggtactat cgttggcata attgatacat
     2041 ccgactacca gctaaagaaa gagcagctga ccgcacagaa aaatgctatt gcatcaaaat
     2101 caggaaacat tgcttcgcag atagaagtgc agcagcaaca aaaaaacaat ctgctgatcg
     2161 acaaggcccg gatagaaaaa ttgtataaag atggcgcagc taccaagaaa caagtggacg
     2221 atattaacgg gagcatcagc atggttgaca aacagataca atcgatccag acacaaaacg
     2281 gcagcgtggc tgacgagata ctcgccatcg taaaacaaat tgaccagata agccagacca
     2341 ttaaaaaatg tcacctctcc tgccctgttt caggaacgat actgaacaaa tatattgagc
     2401 aaaatgaact tgtaattccg ggaaaatccc tgtacaaaat tgccgacctc agcagtgttt
     2461 ttctccgggc ttatatcagt gagtcgcaat tagccaatgt aaaactcggg caaaaggtcg
     2521 aagtgcttat cgataaaggt gaagacaata cggcaaaata tgaaggtacc gtcacatgga
     2581 tatcatcaac agcagagttt acgccaaaaa taattcagac aaaagaagaa cgggtgaacc
     2641 tggtttatgc aataaaaata aaagtaaaaa acgacggagc attgaagata ggtatgccgg
     2701 gagaggtaaa ctttaacgga aaataagagt caggagtcat tccgccgccg cataatatta
     2761 gaaaatgaaa aaacaataaa acaattttac tataaaaaac ccgaatcaat tttacccaat
     2821 caacacaatc aacccattta acccatttaa cttatttaac ttaattccct tttctcccct
     2881 cacgaatgaa caacgtcatc gtagaaaata ttagcaaaag ttataaaaaa gccggtactt
     2941 cgggaaacac tcccattgat aaaataaaaa atgccaggtc ccgcaaaaaa ggattgaatg
     3001 ctgcttcaaa cgaaataaaa gcccttgaca aggtttcttt ccgggtggat agcggcgagt
     3061 tgtttggatt tattggcccc gacggggccg ggaaaacaag cctgttcaga atattggtat
     3121 cgctgctgct gcccgattcg ggaacagcca ccgtatgcgg atttgatgtt gtaaaagatt
     3181 ttaaaaagat ccggaatatc gcaggatata tgcccgggaa attctcactt tatcaggatt
     3241 tgacggtaga agaaaatctg actttcttcg ccacgatctt cggtactaca atacagaaaa
     3301 attatcatct tattaaagac atttactgcc agatcgaacc atttaaagac cgccgtgccg
     3361 gagctttgtc cggggggatg aaacaaaaac tggctctctc atgcgccatg atccacaaac
     3421 ccgaaatcct ttttcttgac gagcctacga cgggcgtgga tgccgtttca cgaatggagt
     3481 tttgggaaat gcttcacaac ttaaaaaaag caggtatcac catcattgtt tcaacgccat
     3541 acatggatga ggcctcactc tgcgaccgcg tggcgctgat acaggaagga agtattttaa
     3601 aaatcggaac ccccgaagaa ataattacag attttcagaa gcccgtgttt gccatacgat
     3661 ctgaaaatac gcaccaactc atccgcgacc tcgaaaaatc tccggaatgt gaaagtgttt
     3721 atcctttcgg gcaatattta cattttatcc cggagaatgt caacattgtg attgacacac
     3781 tttcaaaaac catacatgaa agtcatccgg aagctgaggt ttttcaggtc aaacccgaca
     3841 tagaagattg ctttattaac ctgatgaaag aaaaaaaatg aacggcacag aacccataat
     3901 aaaagtaaga gatcttgtga aaaaattcgg aagtttcacc gcaaatgacc atctcaattt
     3961 tgatgtgtat aagggcgaga tctttgggtt tcttggtgcc aacggcgcag gcaagactac
     4021 ggcgatgaaa atactgtgcg gattgtcgga acccacatcg ggaagtgcca gcgttgccgg
     4081 atatgacgtg tatcgcgagc caaacaaaat taaaaattgc atcggctaca tgagccagaa
     4141 gttttctcta tacgaagacc taacggtaag cgaaaatatc aggttttttg caggtatcta
     4201 cggaatggac cggaaggaaa taaaaataaa aagccggcaa ctgatcgaaa aactaggact
     4261 ttcagatgcc gccgacaaac ttattgccga ccttccgctg ggctggcgtc agaaacttgc
     4321 tttttcggta gcactggtgc atgatccgga gattgtattt ctggatgagc ccaccagtgg
     4381 cgtcgaccct atcacacggc ggcagttctg gacactgatc tatgaagcag ccagtgaagg
     4441 agtgacgatt tttttaacga ctcattatat ggatgaagcc gagtattgcg accgggtttc
     4501 gattatggtt gatggaagga tcacggcatt ggacacacct ggaaaattga aagaacaatt
     4561 taatgctgaa acaatgaatg aggtatttat aaagcttgta agagtaagaa gtgataaatg
     4621 agaggatttt gaaaaaggag aagatttagt tgtatatggt aaagagtagc atggattaag
     4681 tgatgatgat aaatgttctt tggaaaaata aaaattactg attattaatt tagaacttta
     4741 aactatgcat ttttttaaaa atttagaagt ttggaaattg tcgatggaaa ctatagtcga
     4801 aatttataaa gccactgatg catttcctgt aaaagaacaa ttcggattaa tttcacagat
     4861 aaacagaagt gctgtttcaa tagcagctaa tattgccgag ggctgcggca gaaatacaac
     4921 taaggatttc aatcatttcc ttgatatttc acttggttca agttttgaat tggaaacttt
     4981 aattattgcc tcgcaaaata tcggatatct gtctgaagag aaaagcctgt caacaatcaa
     5041 taaaattcat tccattcaaa aaatgttata cgctttgcgc agatcgacaa acaaaccaag
     5101 ataaaaccat accccaatga acaacatgtc aaccttccac ccaccctcta cctttcaacc
     5161 ctctacccgg ctaccattta cctttctacc agctaccttt ctaccctcct ccctcctaac
     5221 atctacctat ccctcctaac atgaaacact tcttcggctt tgttagaaaa gaattccacc
     5281 atattttccg cgaccgtcgg actctgatca tattgttcgg cattcccgta gcacaaatgc
     5341 tgctttttgg atacattgtc accacggaag tcaaagatgc ccgcattgct attctcgacc
     5401 attccaaaga cgaaattacc cgcaaaatag ttgataaatt aagctcctcg ggctacttta
     5461 tcattgcgca acacctcacc aacgagcagg aaatcggaga atgtttccgc aaagggacgg
     5521 taaaagaagt tgttgtcttc gaacagggtt ttgcacaaaa attacaaaaa gaaaaaaccg
     5581 ccccggtaca tcttattgcc gacgcttcgg atgccaattc ggccaatctg gtcgtaaatt
     5641 atactgcagg catcatcaac gattttgttg taaaagaaaa tacctccacc cttatcccca
     5701 tgcaaatagt tacggagccg agaatgcttt acaatcccga gctcaagggg gtgtatatgt
     5761 ttgttcccgg cactatggcc atgatacttg tactgatatg tgccctgcta acttcagtaa
     5821 gtatcgtgcg cgaaaaagaa ctcggaacca tggaagtact tcttgtttct ccgctcaagc
     5881 cgttacagat agttttaggt aaggttacac catacatttt tctttctttt gtcaatgtaa
     5941 tcaccatctt gttgttgagt aattttgttt tcggcctgcc ggtcagggga agtataacat
     6001 tactactggc tgagagtttt ttgtttatat ccctggcatt gtctattggc atacttattt
     6061 cttcctccac aagcagccag cagatggcta tggtggcttc catgctggga ctgatgcttc
     6121 cctccatatt gctttcaggt tttatttttc cgatcgacaa tatgccggga tggattcaat
     6181 ggatatgtta tataaatcca acgacctatt tcattatcat attgaaaaac atcatgctaa
     6241 aagggctggg attcattagt gtctggaaag aaacagccat cctggcagga atgacattgt
     6301 ttttgctgat ggtcagcaca aaaaaattca aggtccggct cggcggaaaa tagcgacata
     6361 accatgagaa ccatactgtt catattacag aaagaattca tacagatacg gcgcaaccgg
     6421 atgatgcttc cggttatttt tgtcgttcca ttgttacaac tgctcattct ggtcaatgct
     6481 gccaacatgg aaatgaagca tataaaaatc tgcatcgtgg acaatgacct gagcagttcg
     6541 tcgcgtctaa tcacaggaaa attcagcaat tcaaaattct ttactttaca gtgccataca
     6601 ttcaacatgg aagatgcccg gcgacagttg gaaaacgacc gtgccgacat tgtccttcat
     6661 attcccgcag ggtttgagcg gggcctggtg cgcgaaaaca aggcaaaagt ccagctgctg
     6721 atcaatgcca tcaacggcac ggtggccggc ataagcaatg cttatgccgc ccagatcctc
     6781 gcaggagcca accgcgagat tatcatgcaa tggtacggaa tcccgaaaac ccaggcagct
     6841 tccgcagcaa ttaacatcat tccggctttc tggtacaatc ctgatatgaa ttacaaaatt
     6901 tttatggtgc cggccatact tgtgctgctg gttactctca tcggaatgat gctggccaca
     6961 ctgaacattg tcagagaaaa agaaatggga aatattgagc agatcaatgt taccccgata
     7021 cggaaaattc actttattac cggtaagctg cttccattct ggatcattgc cttggtgatg
     7081 cttgccattg gtttgcttat cgggaaactg ttatatgaca ttcccatggt tggcagcctg
     7141 cctctcctct ttgttgtggc gggcatatac ctgctggcca tgcttggttt cgggctgatg
     7201 atatcaaaca ctgctgccac ccagcaacag gccatgtttg tcatgttctt ttttatactc
     7261 atttttatca tgatgagcgg catcttcacc cccaccgata gcatgccaca ctgggcacaa
     7321 accattaatt accttaatcc cgtggcctac tttatgcgga tcatcaggat gatactgctt
     7381 aaaggctccg gttttgcaga tatttacagg gacttcctga tactactcgg ttatggaata
     7441 acagtaatta tttttgccgt gatacgctac agaaaggttt cgtaacagga gccaaaagcc
     7501 ccacaaaatc aggtattaat aaaatatttt tgaaaaacag ttgctttaat ccaaagttta
     7561 tgtacctttg cactcacata ttgcgggatg gagcagtggt agctcgtcgg gctcataacc
     7621 cgaaggtcag aggttcgagt cctcttcccg ctactgacgc ggacttttcg gtttttttac
     7681 tggaaagtcc gttttctttt accgcaaaat cacttgaaat acagcgaatt agattaaaaa
     7741 aactgttgta atccttggtt agatatttcc tttttcccgt gtccaagacc aaaccgcttg
     7801 gaaagatcaa tttttgtaat cgcttttttg catcgactgg cacggacacc catattttac
     7861 tgatgttttg aaataacaaa acaacatttt ttattttatt tttttggtta gatatttttt
     7921 ttgaggatat tccaatttct tcctgaattg ccaaaatttc atccgatagc ttaccggaaa
     7981 atttatcata gagttctttt gacaatttcc catatgcaaa tctttcttcc atatcgtcct
     8041 tcgttttgta ttgctctttc agttttgcct tgagcaaagc ttcctcttcc cttctttcat
     8101 tgtattttgc atcaaacagc ttgtttagct gatctctgaa tagcggccca tatttttcag
     8161 gtatcgaaaa accatttaat aaatcaacaa acagttcgtt tgcacccttc cctttggctc
     8221 ttactgtttg gctggcattc atacagacgc ctttacatct tgggcaacaa taataatgta
     8281 cctttttttt cttgacttca taaccc
//
LOCUS       DAOSFP010000060         8298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7361_length_8298_cov_3.927454, whole genome shotgun sequence.
ACCESSION   DAOSFP010000060 DAOSFP010000000
VERSION     DAOSFP010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8298
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7361_length_8298_cov_3.927454"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="PLI16_02890"
     CDS             complement(<1..675)
                     /locus_tag="PLI16_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOH83533.1"
                     /translation="MKRVYKPVVFFLLLFCLLSCNRLKDKDETRTVFRYNESAGISTL
                     DPGFAKDQAHIWVCNQLYNGLVQPDDSMFIRPCVAKSWTVSADGLVYTFVLRDDVYFH
                     DDAVFPKGTGRQVVASDFVYSFGRIANDKIASPGAWIFNDVARDSSGIPCFSAPADTV
                     FVVKLKQPFPPFLSMLQMPYCAVVPHEAVEHYGGDFRSHAVGTGPFKLKRWIENQRLI
                     LVKNDNY"
     gene            complement(874..1782)
                     /locus_tag="PLI16_02895"
     CDS             complement(874..1782)
                     /locus_tag="PLI16_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH83534.1"
                     /translation="MSHQNASVKSILFALLANFGIAVTKTIAAIITGSGAMLAESIHS
                     FADCGNQGLLFLGLRSSKKKPDSEHPLGYGKEIYFWSFIVALILFSMGGLFSIYEGVH
                     KITAHQGLRSPVIALIVLTVSMALEAASLLGCLKQIKKIRKEKKLWQWVRGSRKSELI
                     VVLGEDIAALLGLFFAMLAVILSMITGNPVYDAMGSISIGVLLVIISFLLAFKVKSLL
                     IGQSAEDDTREEIKRFLESRPEIAMIYNIITIQLGPDIMVSVKVKMTQTSSINQMIKD
                     INNCEKELKKHFEAIRWVFFEPDNKE"
     gene            complement(1918..3447)
                     /locus_tag="PLI16_02900"
     CDS             complement(1918..3447)
                     /locus_tag="PLI16_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83535.1"
                     /translation="MKRNVLVCCFLFVTCFSGFSQTTVISHGYTPPASDQVYGWMLDM
                     ANAIRYRLGDAVVRVYNAQSGNFDYLIGSGTQNVLIFDWMTASNNFQSGFSEDAGAAL
                     FTSLIQGYLQGDFDITTLHFIGHSRGTVVNSECAERLLVAGFPVAQTTSLDAHDWGAV
                     SFFDDYDCNPAVWHSGVEAWQGISWADSYYQENLIELNGRPVEGTYQVFLGSIGHNAV
                     HDWYTETILDSTRHEGYYYSINGAGYNNRPAISGTPKTPYFTFTRDGVVNGNFERGNF
                     LNETFPGWSYNGGSGNATIDGNYLGLKSSGACNKVHNRFYIPGNALGIQFDYLIYEED
                     DENPFPEVDRLVVLLNNNPAMNPIMMDSTMTEWASVWLPVANARNSIKTLEFKLVDEC
                     GGSSEISSEVRIDNIKFIIDSTASVMDFAAYCFESMVAPNPCVGNTVVQFNLPKNDKV
                     TIKIYNLMGSEMAVLADDEYSGNSTHKVVFDASSLAAGIYIVNVRVGNSFRNHKVIVS
                     R"
     gene            3875..4741
                     /locus_tag="PLI16_02905"
     CDS             3875..4741
                     /locus_tag="PLI16_02905"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOH83536.1"
                     /translation="MFRKLLLIAALAVFSFQAAAQPNNSLLWEVSGMGLSQKSYIFGT
                     IHMIKKQDFFITPAMVSSLEKCQYFVTEIDMNISLAQQLDLAKKMYLPETKTLRDYIS
                     PDEYTNFSNYVIDSIGIKKSKLEKYIRLKPFFMSSILAKHIAGKIKSYEQELYKLSKK
                     KGMLSDGLESLDFQLSLIEDMPIEEQARTLVSDIQGYKKMEQQLTDMVAAYKNQDLDK
                     LYDFIVKYGDTSPEFNESFIFKRNQNWIPKIEEKIKDHSCFIAVGAAHLPGDKGVLNM
                     LKQKGYTVTPVK"
     gene            4835..5704
                     /locus_tag="PLI16_02910"
     CDS             4835..5704
                     /locus_tag="PLI16_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH83537.1"
                     /translation="MRTASLSIISNSVLIVLKVTIGIISGSVSIISEAIHSGMDLMAA
                     IIAFFSVRISGMPPDKNHQYGHGKFENVSGVIEALLIFIAAGWIILEAVKKIVEPTVP
                     ESLGIGAIVMGISALINFIVSQRLYKVGRETDSVALEADGLHLRTDVFTSLGVAVGLV
                     LIWLTNVAILDPIVAILVALLILRESFILLKKAYYPLLDTPLPSNEIDIIENIITSHL
                     RPGMSFHMLRSRKAGALKYVDLHLEVPGSITVKESHDLCNLIENEIEHQFENIEVYIH
                     IEPSENDPEEKGL"
     gene            complement(5701..7053)
                     /locus_tag="PLI16_02915"
     CDS             complement(5701..7053)
                     /locus_tag="PLI16_02915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOH83538.1"
                     /translation="MKDLTTGNESKVIFSFALPMLLGNVFQQSYNIVDSIIVGNYLGE
                     EALAAVGGSFPVIFVLISLVMGVSIGSTIIIAQFFGAKDFNNVKKAIDSLLIFLFFAS
                     LVLTMLGLIFAENIFRSMNMPEEIIPDAISYFNIMMYGMVIMFGFNAVSSILRGLGDS
                     RTPLYFLIIATVLNIFLVILFVRYFGWGIKGSAIATVVAQGVSFILSIIYLNRFHDII
                     KFSFFDIKFHREIFLKSLKIGIPSGLQQTFVAFGMMALLRFVNGFGTDTIAAYTVAGR
                     IDSFAMQPAMNFSMALSTFVGQNMGANKVDRVKNGYRATLLMTAVFSIVITFVAILFG
                     SQIMSLFTDSGEVVSIGHSYLVIVCSFYILFSTMITTQGLIRGAGDTLIPMFITLMSL
                     WLLRIPLSYVLSRAMGSDGIWWSIPIAWAFGMIASWIYYTTGKWKNKVVTRSIPRSEN
                     "
     gene            7187..8269
                     /locus_tag="PLI16_02920"
     CDS             7187..8269
                     /locus_tag="PLI16_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83539.1"
                     /translation="MKKTLLIICFLSIYSYVAQAQITLDFNDLAVPVKTFDFRKDTAV
                     DPSITPGNPGPNQTWNFMALHSTLDTMTIDVVNSNTIPTGGMFAGSNIATIYDSALIM
                     YFFNSSPLGLVLHGTIYDFFKTGDSIMVELSIPDTALALPGSYGDSATRYSFGDTKSH
                     CNITYDTMGYQVPVDSLRIRHAQVKTSVNDAWGTMMLPGSPNFDVIRQKDYATSQDSI
                     WGHTSFPGFAGWHFLYIRYDTVLTYTWWPKNLGIHAVKFVMGSPTAVVNAEFVYDIDN
                     SISENELSSSTIYPNPASDYITISNTAKYKEVVIFDVLGNEISRHTIGNKDLVRISVE
                     NLPNGLYFFNAINGNTKSSGKFVVKH"
BASE COUNT         2586 a         1897 c         1642 g         2173 t
ORIGIN      
        1 ataattatcg tttttgacta aaatcagccg ttggttttct atccagcgct tcaatttaaa
       61 agggccggta cccacggcat gcgaacggaa atctccgcca tagtgctcca cggcttcgtg
      121 cggtactaca gcacaatagg gcatttggag catactcaaa aaaggcggaa aaggctgttt
      181 tagttttaca acaaataccg tatcggcagg agcggaaaaa caaggtattc ctgatgaatc
      241 ccgcgccaca tcattaaata tccatgctcc gggcgaagct attttatcat tggcaatacg
      301 accaaagcta taaacaaaat ccgaagccac cacctgccgt cctgtgcctt tggggaaaac
      361 agcatcatca tgaaaataca catcatcgcg taaaacaaaa gtataaacca gcccgtcggc
      421 agatacagtc caggatttgg caacacaagg cctgataaac atggagtcat cgggttgtac
      481 caacccatta tacagttggt tgcacaccca tatatgcgcc tgatcttttg cgaatccagg
      541 atcgagggtg cttatgcccg ccgattcgtt gtagcgaaaa actgtccggg tctcatcctt
      601 atcttttaac ctattgcagg ataataaaca gaataacagc aggaaaaata ctaccggttt
      661 atataccctt ttcatgatac ctgtttaaaa gttcaaatat attccaatta tcggcacagt
      721 gcataacgca tcgtttttaa taataaacaa tgcattgtta aaactcattg aaaaattttc
      781 attacagctt acatgcagct attgacagtt tataaacttc aaccataacg aacactttgg
      841 catatcccgc tgtgcagagc ttttaccggc ggattattcc ttattatcag gttcgaaaaa
      901 cacccaccgg atggcctcaa aatgtttttt aagttccttt tcgcagttgt taatatcttt
      961 gatcatctga ttgatggaac ttgtctgcgt catttttact ttcacggaaa ccatgatgtc
     1021 aggccctaac tgtatcgtta ttatgttgta aatcattgct atttcagggc gtgattccaa
     1081 aaaacgcttt atctcttcac gggtatcatc ttcggcactt tgccctatca gcaagctttt
     1141 cactttaaaa gcaagaagaa acgaaatgat taccagcaaa acaccgatgc tgatacttcc
     1201 catcgcgtcg tacacagggt ttcccgttat catggacagt atcacggcca gcatggcaaa
     1261 aaacaatccc agcagggcgg caatatcctc gcccagcacc actatcaact cgcttttacg
     1321 gcttcccctg acccactgcc acagtttttt ttcttttctg attttcttta tttgtttcag
     1381 gcaacccaac agtgatgcag cctccagcgc catactaacc gttaacacta tgagggcaat
     1441 aacaggactt ctaagcccct ggtgggccgt aattttgtga acgccctcat aaatcgaaaa
     1501 cagtccaccc atgctgaaca aaatcagagc tactataaaa gaccagaaat atatctcttt
     1561 gccatatccc aaagggtgtt cgctgtcggg ctttttcttt gatgaccgca aaccaagaaa
     1621 cagcaaaccc tggttgccgc agtcggcaaa agagtgaatt gattcggcca gcatagcgcc
     1681 cgaaccggta attattgccg caattgtttt ggtgacagct ataccgaaat tggctagcaa
     1741 agcaaaaagt atagacttga ccgaagcgtt ctgatgagac attatggaat gttttgacag
     1801 gctatttcgt gaaacaaaat atgtttttcg tatgtttaat caattattgt tcaaatttaa
     1861 agaataatcc gaagcaaggc aacgtcacaa acaatttgta gaggttgaag tgcgagctta
     1921 ccgggaaaca ataaccttat gatttcggaa actatttcct acacgcacat taacgatata
     1981 aatcccagcc gcaagtgagc tggcatcaaa taccactttg tgagtgctgt tgccggaata
     2041 ttcatcatca gccaggacgg ccatttcgct gcccatcagg ttatagattt ttattgttac
     2101 cttatcattt ttcggtaggt tgaattggac aacggtattg ccaacacatg gattgggggc
     2161 taccatgctt tcgaagcagt aagcggcaaa atccataacc gaagcagtag agtcgatgat
     2221 aaatttaata ttgtcaatcc tcacctccga gcttatctca ctacttccgc cacattcgtc
     2281 cacaagttta aattccagtg tttttatact attccgggca ttggccacag gcagccaaac
     2341 actggcccat tcggtcatgg tgctatccat cataatgggg ttcatagccg gattattgtt
     2401 gagtaaaacc accagcctat ccacttcggg aaaaggattt tcatcatctt cttcataaat
     2461 aagataatca aactgaatac ccagggcatt gccggggata taaaaccggt tatgcacctt
     2521 gttgcaggca cctgaggatt tcaaaccgag atagttacca tcgatagttg catttccgct
     2581 accgccatta taagaccatc cgggaaatgt ttcattaaga aaattgcctc gttcaaaatt
     2641 tccatttacc acaccatcac gggtaaaggt aaagtaaggc gtttttggtg taccgctgat
     2701 ggctggcctg ttgttgtatc ccgcgccgtt tatggaataa taatatcctt cgtgccgcgt
     2761 agaatccagt atggtttcgg tgtaccagtc gtgtaccgca ttatgtccga tacttcccag
     2821 gaaaacctga tatgtgcctt ccacaggcct tccgttaagt tcaatcaggt tttcctgata
     2881 ataagagtct gcccaactta tgccctgcca ggcctccacg ccggagtgcc aaacggcagg
     2941 attacaatca taatcatcaa aaaagctgac agcgccccag tcgtgggcat ccaacgaggt
     3001 ggtttgagca accgggaaac cagccaccag caggcgctcg gcacattcgc tgttcactac
     3061 cgtaccgcgg ctgtgtccta taaaatgcaa tgtagtgata tcaaaatctc cttgcagata
     3121 gccttgaatc agcgaggtaa acaaggccgc accggcgtct tcgctaaaac ccgactgaaa
     3181 attgttgctt gccgtcatcc agtcgaagat tagcacattc tgtgtccccg agcctataag
     3241 ataatcaaaa ttgcctgact gcgcattata caccctgaca acggcatccc ccagcctgta
     3301 gcgaatggcg ttggccatat cgagcatcca gccatacacc tggtcggagg cgggtggagt
     3361 ataaccatgc gaaataacgg tagtttgtga aaaaccagaa aaacacgtta caaacaaaaa
     3421 acaacagact aatacatttc ttttcataaa acacaaatgg tataacacat caaattcaca
     3481 aaattaaata tttttaatgt gcaaaattat aattttcaaa aagagattac ttccctttgg
     3541 ctattctgga aacaacaacc atgtgcttta ccaaagaggt aatcatacaa gaaaaattta
     3601 tgagtagtta gcctgaaagg aaaacagcct cgccccgtct ttcctgccgg ccgaaacatc
     3661 agtgcacaca ggcaggcggc cttcgtgtct gccccgacgg actcagttca agtatcaaat
     3721 gtagaaagtg aaaagtatta tcatctgcta tagtttgtat atcaatgggt acaagcaata
     3781 cagaaaacct tcaaaatata atatatcata agacaacatt tcgttataaa atgcactagt
     3841 gaatttgatt aatactacct ttgtcaaaaa aaacatgttc agaaaattat tactgatagc
     3901 tgctttggca gttttttcgt ttcaggccgc cgcgcaaccg aacaactccc tgctttggga
     3961 agtttccggt atgggactga gccagaaatc ctatattttc ggaacgatac acatgattaa
     4021 gaaacaagat ttttttatta cccccgccat ggtatccagc ctggaaaaat gccagtattt
     4081 tgtaactgaa atagatatga acatttcgct ggcacaacaa ctcgacctgg caaaaaaaat
     4141 gtacctacct gaaaccaaaa ccctgcgcga ctacatatca cccgatgagt acactaattt
     4201 cagtaattat gttattgatt ccatcggaat taaaaaatca aaactggaga aatatatcag
     4261 gctgaaacct ttttttatga gttcgattct tgccaaacac attgccggaa aaatcaaatc
     4321 gtatgaacaa gaattatata agctatccaa aaagaaaggg atgttatccg atgggcttga
     4381 gtcgctcgac ttccagctat cccttattga ggatatgcct atcgaagaac aggccaggac
     4441 tttggtttcg gacattcaag gatacaaaaa gatggagcag caattgacgg atatggtagc
     4501 cgcatataaa aatcaggatt tggataaact atacgatttt attgtgaaat atggcgacac
     4561 cagccctgaa tttaatgaaa gctttatttt taaacgcaac cagaactgga tacctaaaat
     4621 tgaagaaaaa ataaaagatc acagttgttt tattgcagta ggtgcagccc atttaccagg
     4681 agataagggt gtgcttaaca tgcttaaaca gaagggatac acagttacac ctgtaaaata
     4741 aaactcctta ttagtatttt tatactttca aaataacaca acatagaatt ttataaaata
     4801 caaaatgcag cgcaagtcat tacagcgaaa aaaagtgagg acggccagtt tgtcgataat
     4861 ctcaaactcg gtgctgatag tactcaaggt aaccatagga ataatcagcg gttcggtaag
     4921 tattatctcc gaagccatcc attcgggcat ggacctgatg gctgccatca tagctttttt
     4981 ctcggtacgc atttcgggta tgcctccaga caaaaatcat cagtacgggc acggaaaatt
     5041 tgagaatgtt tccggagtta ttgaagcatt attgattttt attgccgccg gctggattat
     5101 acttgaagct gtaaaaaaaa tcgtagagcc caccgtgccg gaatcattgg gtataggggc
     5161 aatcgtcatg ggtatctccg cgttgataaa ttttattgtt tcgcaacgtc tttacaaggt
     5221 aggaagagaa accgattcgg tggcgcttga agccgacggg ctgcatttga ggacagatgt
     5281 atttacatca ttgggagtag ctgtgggact tgtactgata tggcttacca atgtagcaat
     5341 tcttgacccc atcgtcgcca ttttggtagc cttattgata ctgagagaat cattcatttt
     5401 gctgaaaaaa gcctactatc ccctgctcga cacgccgctg ccttcaaatg aaatcgacat
     5461 aattgaaaac atcattacca gccacctccg gcccggaatg agttttcaca tgttgcgttc
     5521 ccgcaaagcc ggcgccctta aatatgtgga tcttcatctc gaagtacccg gaagcatcac
     5581 cgtcaaagaa tcacatgacc tgtgcaacct gatcgaaaat gaaatcgagc atcagtttga
     5641 aaatattgaa gtttacatac atatagagcc aagcgaaaac gacccggaag aaaaaggcct
     5701 ttaattctcc gagcggggaa ttgacctggt aaccacttta tttttccact tgccggtggt
     5761 ataataaatc cacgatgcta tcatcccaaa agcccaggca atagggatgc tccaccatat
     5821 tccatcagac cccatggccc gcgaaagcac atacgaaagc ggaatcctga gcagccaaag
     5881 cgacatcagg gtgataaaca tgggtatcaa ggtatcaccg gcaccgcgaa tcagcccctg
     5941 cgtagtaatc atcgtggaaa aaagaatata aaaagaacac acaattacta agtacgaatg
     6001 tccgatgctc accacctcgc cactatcagt aaacaacgac atgatttgtg acccaaacaa
     6061 tatggccacg aaagtaatga cgatagaaaa gacagcagtc atcagcaagg tagcgcgata
     6121 tccatttttt acccggtcaa ctttgtttgc tcccatattc tgccctacaa aagtggaaag
     6181 tgccatcgag aagttcatag ccggctgcat ggcaaaagaa tcaattcgcc cggctaccgt
     6241 ataggcagcg atggtgtcgg taccaaagcc attgacaaaa cgcagcaagg ccatcatgcc
     6301 aaaagcgaca aaagtttgct gtaagccaga cgggatgcca attttaagac ttttcagaaa
     6361 aatttcacgg tgaaacttaa tgtcgaaaaa cgaaaactta atgatatcat gaaaacggtt
     6421 aaggtaaatg atgctgagaa taaaggacac cccctgcgct accactgtgg caatagcaga
     6481 gcctttgatg ccccatccaa aatacctgac aaaaagaata accagaaaga tattcaaaac
     6541 cgtggcaata atcaaaaaat acaagggtgt cctggaatcg cccaaccccc ttaaaatgga
     6601 acttacagca ttgaatccaa acatgatgac catcccgtac atcatgatat taaaataact
     6661 gatagcgtcg ggaataattt cttcgggcat attcatcgac ctgaagatgt tttcagcgaa
     6721 tatcaatccc agcatcgtca ggaccagaga agcgaaaaac agaaatatca acaggctgtc
     6781 gatggccttt ttgacattat taaaatcttt agccccaaaa aattgtgcaa taatgatcgt
     6841 agatcctatt gaaacgccca ttaccagcga aataagcaca aatataaccg ggaaagaccc
     6901 acctacagca gccagcgctt cttcgccaag gtaattaccc acgatgatac tgtccacaat
     6961 attatacgac tgctgaaaaa cattgccgag caacattggc agggcaaaac tgaaaatcac
     7021 tttactttcg ttgcctgtgg tcaggtcttt catattaggg ataaaagttt gcaaagataa
     7081 tattttccat gcggctatcc ggcactttgg tattttttgt ggaataacga agtattttca
     7141 atattttatc ataaatttgt cctgaacaat aaaaaacaca tttatcatga agaaaacatt
     7201 acttattatc tgcttcttaa gcatttattc ttatgttgcc caggcacaaa tcactcttga
     7261 ttttaacgat ttggctgtac cggtaaaaac gttcgacttc agaaaagaca ccgctgttga
     7321 cccgtcgatt acacccggca acccaggtcc taaccagacc tggaatttta tggcactgca
     7381 cagcaccctc gacaccatga ccattgatgt tgtcaactcg aacacaattc ctaccggcgg
     7441 gatgtttgcc ggcagcaaca ttgccaccat ttacgacagc gctctgatca tgtatttttt
     7501 caacagttcg ccgctgggcc tggtgttgca tggcaccatt tatgattttt tcaaaaccgg
     7561 cgactctatt atggtagaac tgagtatacc cgacacagca ctggcacttc ccggttcgta
     7621 cggcgattcc gctaccaggt atagtttcgg cgatacaaaa tcacattgca acattactta
     7681 cgacaccatg gggtatcagg tcccggtgga ttctttgcgc ataagacatg cacaggttaa
     7741 aacatcggtc aacgatgcct ggggcaccat gatgttgccc ggcagcccta actttgatgt
     7801 catcagacaa aaagactacg ccaccagcca ggactctatc tggggacata cgagtttccc
     7861 agggtttgcc ggatggcact ttttgtatat ccgttacgac accgtgctta cctatacctg
     7921 gtggccaaaa aatctgggca tccatgccgt gaaatttgta atgggatcac ctaccgccgt
     7981 tgtgaatgca gaatttgttt atgacatcga taacagcatc agtgaaaacg agttatcgtc
     8041 atcaactatt tacccgaacc ccgccagcga ttatatcacc atttccaata ctgcaaaata
     8101 taaagaagtg gttatattcg atgttctcgg aaatgaaatt tcaaggcata ccatcgggaa
     8161 caaagacctg gtgaggatat ccgttgaaaa tttaccgaat ggattgtatt ttttcaatgc
     8221 cataaatggg aacaccaaat ccagtggaaa atttgtggtt aagcattaat tcacacagaa
     8281 aataagaaaa aaaccgga
//
LOCUS       DAOSFP010000061         8267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7399_length_8267_cov_3.943497, whole genome shotgun sequence.
ACCESSION   DAOSFP010000061 DAOSFP010000000
VERSION     DAOSFP010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7399_length_8267_cov_3.943497"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="PLI16_02925"
     CDS             complement(<1..1343)
                     /locus_tag="PLI16_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83540.1"
                     /translation="MIRKVLLLLISCGSLLVTNTFAQPVITWQKSIGGSGLDFGKGLC
                     VAGDSLSYYLTGYTNSANGDMTTNLGGTDIFLSKINLSGTVLWTRTFGTSADDYAHSV
                     ISSGDHGVIICGFTRDSVTQGTHGGFDVLLIKADSSGNQQWMKFYGGSRNDGDHFAEI
                     IQTQDKGIVCITGAASFDGDLDTNYGSTDYWLFKTDSAGNLLWEKNFGGSDDEDGHNV
                     LPMPDGGFIINGHTASNDADVSGHHNPGSGIHDGWIVRVGPSGNIIWQKCIGGTGFEL
                     VNQMVMDDSGHIVMTGYTSSTDGDVSGNHGMNDVWIVTMDTLSGSLLSSEVYGGPGDD
                     YGLYLLKQAGGSLKCGGYSNSSTGEVTGNHGNFDYWVFSPDQTGGIIIWGNCYGGSSN
                     DRIGGIMQSTENGIICYGYSNSSNGDVVGNHGLFDCWVFQIDCQEPSASFTCNPSP"
     gene            1623..4931
                     /locus_tag="PLI16_02930"
     CDS             1623..4931
                     /locus_tag="PLI16_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83541.1"
                     /translation="MGKHLKIWATLSLLMLSGFSAFAQFYNGSQLNFGKNRIQFTDRF
                     WSFMRFEKFDTYFYLNGKELAVYTAKYADKYLKEIEKKLEYTLEDKIQFIIYNKFSEL
                     QESNIGLIQDVNYNIGGMTHIVGTKVFLYYNGSHTDFEKQIRAGMAQVIIDEMMYGGK
                     ITSMIKNSALMSMPAWYSQGLVSYISEEWSADIDNYVKDGILRGDYKRYNSLSGNDAI
                     YAGHSIWKYVADKYGPSAIANIVYLSKVSRNVESGFLYVLGVSFKNLAKEWLAYYEQM
                     YRDGDQGRQNYQETALLKKFKKNYVYGNIKISPDAKYAAYTTNYMGQYRVWLFDFEKN
                     KTRKILQRECRLDEKVDYSFPLLAWHPTGKMLAIIIETKGKNMMYSYMMDEKKFEKQQ
                     LFNLEKILDLSFSQNGKMIVFSGVVQGQTDIYVYNVAAHTYEPITKDIYDDLNPRFAN
                     NSTEIIFSSNRPDDTIRYDVLTYLESERDTITHLLPYTDIFIYDYKTKSRLLKRLTNT
                     PHTNEIMPLPYDSRFYSYLSDENGIYNRYLARIDSTISYIDTATHYRYYTTSFATTDY
                     SRNILQHDISPAAKKSAEVIFSDGRHRLFVNDLPQAKDFKKMTPANTFNRTIFLNNLQ
                     QEARGDNSLINTEEIEILKNKKTKTKPKKPANVLVDQLNNEPDTGAVDINNYVFSGEQ
                     KSLTATDQDSLGLKFKKTFTLPRQQNYNVEYSINQLVGQIDFNYLNTSYQQFTGGGSP
                     VYLNPGFNVFFQLGVIDLLEDYRISGGISLSLSFDNNEFFIGFENLKKRLDHGFFYYR
                     QANTGTNGYSLIKLRSNSLFYVMKWPFSRVLAIKGTVLGRYDKMIYASTDYYNLQIPD
                     VHEFWTGAKAELVFDNTQYKAINIYYGWRWKIFGEYYQKLDKKFNNLVVLGMDFRHYQ
                     KIHRTFIWANRLAASTSFGNNKLVYYMGGVDTWIIPRFNNENQVSQTQNYAYQTLATN
                     MRGFVQNVRNGPTFAVINSELRFPIVRYFSKKPLKDNLFTNLQIVGFGDIGTAWTGLN
                     PYSDENVITRQIVQQGPITVTLIKKRDPIIGGFGFGLRTKLLGYFIRADYAWGVENGL
                     LQKPVFYISLSTDF"
     gene            4949..6190
                     /locus_tag="PLI16_02935"
     CDS             4949..6190
                     /locus_tag="PLI16_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOH83542.1"
                     /translation="MKDLKSKIKELSREYAPEIITLRRHFHAYPELAFEEIKTGDIIC
                     AELDKAGIPYRRGIAKTGIVALIKGKNPDKKAVGLRADMDALPMTETTNADYSSKNEG
                     RMHACGHDVHMASMIGTAIILNRLKDSFEGSVKIIFQPSEEKYPGGAIQMIMEGVLEN
                     PKVSCMIGQHVHPSVDAGKAGFKHGKSMASTDEVYINVLGKGGHAATPELVIDPIVVA
                     SHIIVGLQQVVSRMATPTIPTVLSFGRIFANGKTNIIPDEVFIEGTFRTVDENWRKTA
                     HEKIVVAAQNIASAFGATCKIFIDKGYPFLFNDETLTHRIMHYAEDYLGNKNVQEIDL
                     RMTAEDFAYFSHTLPSCFYRLGVKDPKSKEISNLHTPGFTVAENSLETGMGLMAWAAI
                     NELNHKEEPATTKKTMPPRKH"
     gene            complement(6156..7052)
                     /locus_tag="PLI16_02940"
     CDS             complement(6156..7052)
                     /locus_tag="PLI16_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH83543.1"
                     /translation="MFSEHVGEFAALFTACCWTVTALAFESASNKVGSIAVNLLRLLL
                     AMVFLSVFTYFTRGRFFPDDAGSNTWFWLVLSGLIGFVVGDLFLFESYTIIGSRVAML
                     IMTLVPPMTAFLSWLMLGETLTLFNLFGMSLTLGGIVLVVVNKGKGEKMFSFAHSLKG
                     ILFAFIGAVGQAIGLVFSKVGMGGYNAFAATQIRIITGIIGFSIVITFWKKWQNVGLA
                     IKNVSAVKRIGLGSVFGPFLGVSFSLYAVQHANTGIASTIMAIVPVLIIPASVLFLHQ
                     KVTLKDVIGAAISVCGVALFFL"
     gene            complement(7138..>8267)
                     /gene="kbl"
                     /locus_tag="PLI16_02945"
     CDS             complement(7138..>8267)
                     /gene="kbl"
                     /locus_tag="PLI16_02945"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008025472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOH83544.1"
                     /translation="KKCLNFCANNYLGLSSHPELIKAAHKEIDNRGYGLSSVRFICGT
                     QDIHKELERKVSEFLGMEDTILFSSCFDANGAVFEPLLGENDAIITDALNHASIIDGI
                     RLCKAQRWIYKHGDMRDVEEIDPANDKMAKGLERCLKEAREKGCRFVMIATDGSFSMD
                     GDIAKLDEICDLAEKYDALVMVDDSHSTGFLGKTGRGTHEYRGVMGRVDIITTTFGKA
                     LGGASGGCISSSKEIVSWMRNKARPYLFSNTVAPSVVGATIKVIDILTKSTELRDKLE
                     RNTKYFREKMTAAGFDIIPGEHPIVAIMFGKYPDCSKLAVDFANKMLDEGIYVTAFSF
                     PVVAKGKDRIRVQISAGHEMEHIEKAIAAFTKVGKELGMLK"
BASE COUNT         2480 a         2058 c         1660 g         2069 t
ORIGIN      
        1 ggactgggat tacaagtgaa tgatgcagat ggctcctggc agtcaatctg gaacacccaa
       61 caatcgaaca gcccatggtt tcccaccaca tcgccattgc tggaatttga atacccataa
      121 catattattc cgttttcagt actttgcata atacctccaa tgcggtcgtt gctgcttccc
      181 ccgtaacaat ttccccaaat aataatacct cccgtttgat ccggtgaaaa aacccaataa
      241 tcgaaattgc catgatttcc ggtaacttca ccggtggaag agttggaata gccgccgcac
      301 ttcaggctgc ctccggcctg cttcaggaga taaagtccat agtcatctcc cgggccgcca
      361 tatacttccg aagacaacaa actccccgac agggtatcca tggttactat ccacacatcg
      421 ttcatgccgt ggttgcccga aacatctcca tccgtagagg aggtatatcc tgtcatcaca
      481 atatggcccg aatcatccat caccatctga ttgaccagtt caaagcccgt accgccaata
      541 catttctgcc agataatatt ccctgatggc cccacacgca ctatccatcc atcatgtatt
      601 ccgctgccgg gattgtggtg cccgctaaca tccgcatcat tggaagcggt gtgaccgttg
      661 atgataaaac cgccatcagg catgggcaat acgttatgcc cgtcttcatc atcgctgcct
      721 ccaaagtttt tttcccataa aagattacct gccgaatctg ttttaaaaag ccagtaatca
      781 gtacttccat agttggtatc cagatcgccg tcgaaagaag ccgcaccggt aatacacact
      841 atgcccttgt cctgtgtttg aataatttca gcaaaatgat ccccatcatt acggctgccg
      901 ccataaaact tcatccattg ctgatttccg gaagagtcgg cttttatgag caacacatca
      961 aatcccccgt gggttccttg tgttacgcta tctctggtaa acccgcaaat gatgacacca
     1021 tggtcacctg aagaaataac cgaatgtgca taatcatcag cactggtgcc aaaagtacgg
     1081 gtccataata cagttccgct aagatttatc ttactcagaa aaatatcggt tccgccaaga
     1141 ttggttgtca tatcgccgtt ggccgaattg gtgtatccgg taagataata cgacagcgaa
     1201 tcccctgcca cacatagtcc tttgccaaaa tcaaggcccg agccgcctat acttttctgc
     1261 cacgtaatta caggctgcgc aaaggtgttt gtaacaagca ggctgccaca acttattaat
     1321 aataataaaa cctttcttat cattacaaaa aaaagtatca atattatttt caaatataac
     1381 ccattttaaa gaaaaaacta aaaataattt ttgtcgtcct ttaattcagg atactgcatc
     1441 acaaaaaaac tcccggtggt tcaaacagtg catctttttc aaaattcccg gttgttgacg
     1501 caccaacagc aatacaagga acatatcaac tcccgggttt tgtccgttgc ctacttttta
     1561 aatgattttt gcaacaacaa tatgaaaagt ttgtaacttt acactattat taatagccat
     1621 tcatgggtaa gcacctgaaa atatgggcga cactgtcatt actgatgcta tcgggctttt
     1681 cggcttttgc tcagttttat aacggctcgc agcttaactt tggcaaaaac cggattcaat
     1741 ttaccgatcg tttttggtca ttcatgcgtt ttgaaaagtt tgatacgtac ttttacctca
     1801 atggcaagga actggccgtt tataccgcca aatatgccga taaatacctg aaagagatag
     1861 aaaaaaaact cgaatatacg ctcgaagaca agatacagtt tatcatctat aacaagttct
     1921 ctgaacttca ggaaagcaac ataggcctca ttcaggatgt taattacaac atcggcggga
     1981 tgacacatat tgtgggcacc aaggtttttt tgtattataa cggcagccac actgattttg
     2041 aaaaacagat acgtgccggc atggctcagg tgatcatcga cgaaatgatg tatggcggca
     2101 aaatcacctc catgataaaa aattcggcgt tgatgtccat gcctgcctgg tattcacagg
     2161 gactggtgtc gtacatttcc gaagaatgga gcgccgacat cgacaattac gtaaaagatg
     2221 gcatactgcg cggcgactac aagcggtaca acagcctttc gggcaacgac gctatctatg
     2281 ccggtcattc catctggaaa tatgtggccg acaaatacgg cccctcggca atagccaata
     2341 ttgtttacct gtcaaaagtc agccgtaatg tggaaagcgg ttttctgtat gtactcggtg
     2401 tatcctttaa aaacctggcc aaagaatggc tggcatacta tgagcaaatg taccgcgacg
     2461 gtgaccaggg acgccagaat taccaggaaa cagccctgtt gaaaaaattc aaaaaaaact
     2521 atgtttacgg aaatataaaa atcagtcccg atgctaaata tgccgcctat accaccaatt
     2581 atatggggca ataccgtgtt tggctgtttg attttgaaaa aaataaaacc cgtaaaattt
     2641 tgcaacgcga atgccggctc gacgaaaagg ttgactactc ttttcctctg ctggcctggc
     2701 atcccacagg aaagatgctg gccatcatca tagaaaccaa aggcaaaaac atgatgtatt
     2761 catatatgat ggatgagaag aaatttgaga aacaacaatt gttcaacctc gaaaaaattc
     2821 tggacctttc cttttcacaa aacggtaaaa tgatcgtgtt ttcaggggtg gtgcagggtc
     2881 aaaccgatat ctatgtgtac aacgtggccg ctcatacata cgagccaata accaaagaca
     2941 tctacgacga tcttaaccca cgttttgcaa ataattccac cgaaatcata ttcagttcca
     3001 accgccctga cgacactata aggtatgacg tgctcactta cctcgaatcg gaaagggata
     3061 ccatcactca cctgctacca tatacggaca ttttcattta cgattacaaa accaaaagca
     3121 ggttgctgaa acgccttacc aacacgcccc atacgaatga aattatgccc ctgccctacg
     3181 actcccggtt ctattcatat ctttcggacg aaaacggcat ctacaaccgt tacctggcac
     3241 ggattgacag caccataagt tatatcgaca cagcaaccca ttaccgctat tacaccacat
     3301 cctttgcaac caccgattat tcaagaaaca tcctgcaaca cgatatttcc cctgccgcca
     3361 aaaaatccgc ggaagtgatt ttttctgatg gcaggcaccg gctttttgtc aacgaccttc
     3421 cccaggccaa agactttaaa aaaatgacgc cggccaacac cttcaaccgc accatttttc
     3481 tgaacaacct gcagcaggag gcccgcggcg acaactcatt gatcaatacc gaagaaattg
     3541 aaatactcaa aaataaaaaa acaaaaacaa aacccaaaaa acccgccaat gtccttgttg
     3601 accagttgaa taatgagccc gatacgggag ccgtggatat caacaattat gtgttcagcg
     3661 gagaacaaaa atccctgaca gctactgacc aggattctct tggtttaaaa tttaaaaaga
     3721 cttttaccct gccacgtcag caaaactata acgtggaata ctccatcaac cagcttgtag
     3781 ggcagataga tttcaattac ttgaacacct cctaccagca gtttaccggc ggcggctccc
     3841 cagtgtatct caatcccggg ttcaatgtgt tttttcaact cggcgtgatt gacctgcttg
     3901 aagattacag aatttcagga ggcatcagcc tttcactgag ttttgacaat aacgaatttt
     3961 ttatcggatt tgagaacctg aaaaaacgcc tcgaccacgg atttttctat tacaggcagg
     4021 ccaacacagg cacaaatggt tattcgctca tcaaactgcg ctccaactcc ctgttttacg
     4081 ttatgaaatg gcctttttca agggtattgg ccatcaaagg aactgtgttg ggacggtacg
     4141 acaagatgat ttatgcttcc accgattatt ataacctgca aatccccgat gtccacgaat
     4201 tctggaccgg cgccaaagca gaactggtct tcgacaacac gcaatacaaa gccatcaaca
     4261 tctattacgg ctggcgctgg aaaattttcg gagagtacta ccaaaaactc gataaaaaat
     4321 tcaacaacct ggtggtgctt ggaatggatt tccgccatta ccaaaagatt caccgcacct
     4381 ttatctgggc caaccggctg gctgcaagca cctcttttgg caacaacaaa ttggtttatt
     4441 atatgggtgg tgttgacacg tggatcatcc ccaggtttaa caatgagaac caggtttcgc
     4501 aaacacaaaa ctatgcctac cagaccttag ccaccaatat gaggggattt gtgcaaaatg
     4561 tccgaaacgg tcctaccttt gccgtcatca acagcgaact gcgattcccc atcgttcgtt
     4621 atttctcaaa gaaacccctc aaagacaacc tgttcaccaa cctgcagatc gtcggtttcg
     4681 gagatatagg caccgcctgg acaggattaa acccttattc cgacgaaaac gtgattaccc
     4741 ggcaaattgt gcaacaaggg ccgattaccg taaccctcat caaaaaacgc gaccccatta
     4801 ttggcggatt tggctttggc ctgaggacaa aacttttggg gtattttatc cgggccgact
     4861 atgcctgggg cgttgaaaac ggtctattgc agaaacctgt tttttatatc tcgttaagca
     4921 ctgattttta acctacgaca ttttttctat gaaagactta aaatctaaaa tcaaagaact
     4981 gtccagggag tatgctcctg aaataataac cctcagaaga cattttcatg cctatcccga
     5041 actggctttc gaagaaataa aaaccggcga cataatctgt gccgagctcg acaaggccgg
     5101 catcccctac aggaggggta ttgccaaaac cggcattgta gcgcttatca agggaaaaaa
     5161 tcccgacaaa aaagccgtag gcctgcgagc cgatatggat gccctgccca tgaccgaaac
     5221 cacaaatgcc gactacagct ctaaaaacga aggccgtatg catgcctgcg gacacgatgt
     5281 gcacatggcc tcgatgatag gtactgcaat tatcctgaac cgcctgaaag actcctttga
     5341 aggcagtgtt aaaattatct ttcagccatc agaagaaaaa tatcccggtg gcgccataca
     5401 aatgattatg gaaggagtgc tggaaaaccc caaagtcagc tgcatgatag gtcaacacgt
     5461 acaccccagc gtagatgcgg gcaaggcggg tttcaagcac ggaaaatcaa tggcttcaac
     5521 cgatgaagtg tatatcaatg tgttaggcaa aggcggacat gctgccaccc ccgaactggt
     5581 aattgacccc atagtagtgg cttcgcacat catcgtagga ctgcagcagg tggtaagccg
     5641 tatggccacc cctaccatcc ccaccgtgct ctcattcgga cggatatttg ccaatggcaa
     5701 aaccaatatt attcccgacg aggtgttcat cgaaggtacg tttcgcacgg ttgacgaaaa
     5761 ctggcgcaaa acagcccacg aaaaaatcgt cgtagctgca caaaacattg cttcggcttt
     5821 tggagccacc tgtaaaatct ttattgataa aggctacccg tttttattta atgatgaaac
     5881 cctcacacac cgcattatgc attatgccga agattatctg ggcaataaaa acgtgcagga
     5941 aattgacctg aggatgaccg ccgaagattt tgcctatttt tcgcatacgc tgccatcatg
     6001 tttttaccgc ctgggcgtta aagatcccaa aagcaaagaa atatcaaacc tacatacacc
     6061 ggggtttacc gttgccgaaa acagccttga aacaggcatg ggcctgatgg cgtgggctgc
     6121 tatcaacgag ctgaaccata aagaagaacc ggctactaca aaaaaaacaa tgccaccccg
     6181 caaacactga tagcggcgcc aattacatcc ttcaacgtaa ctttctgatg taaaaacaac
     6241 accgaggcag ggataataag cacgggaaca atagccataa tagttgaagc tatacctgta
     6301 ttggcatgct gaaccgcata cagcgaaaat gacacgccca gaaaaggccc gaaaactgag
     6361 cccaggccga tgcgcttgac tgcagaaaca ttctttatag ccagccccac attttgccat
     6421 ttcttccaga aagtaatcac aatagaaaaa ccgataatcc cggttatgat gcgtatttgc
     6481 gtagcggcaa atgcattgta accgcccatg cccaccttgc tgaacaccag gcctatagct
     6541 tgccctaccg cgccgataaa agcaaacagg attcctttaa gagaatgagc aaatgagaac
     6601 attttttcac ctttgccttt gttaacaacc accagcacta tgccgcccag ggtgagcgac
     6661 atcccgaaaa gattaaataa tgttagcgtt tcgcccagca tcagccaact gagaaaagcc
     6721 gtcataggcg gcaccagcgt catgataagc atggccaccc gcgaaccgat aatagtgtat
     6781 gactcaaaca aaaacaaatc gccgaccaca aaacctatca ggcctgaaag caccagccag
     6841 aaccaggtgt tactgccagc atcatccgga aagaacctgc ctctggtaaa atatgtaaaa
     6901 acacttaaaa acaccatagc cagtagcaag cgcagcaggt tcaccgctat ggagcctact
     6961 ttgttgctgg ccgattcaaa agcaagggcg gtaactgtcc agcagcaggc cgtaaacaat
     7021 gcagcaaatt ctcctacgtg ttcagaaaac ataataaaag gcttttataa caaattatgt
     7081 attcagataa aaaaagggca acaagcgctg cccttttaat tttcctatct ctcgctacta
     7141 ttttaacatt cctaattctt taccaacctt tgtaaaggcg gcaatagctt tttcgatgtg
     7201 ctccatttcg tgtcctgcag agatctgcac acggatacgg tctttacctt tggccaccac
     7261 cgggaacgag aaagcagtta catagatacc ttcatccagc attttgttgg caaagtcaac
     7321 ggccaatttc gaacaatccg gatatttccc aaacatgatt gctacgattg gatgttcgcc
     7381 gggaataata tcaaaacctg cggctgtcat tttttcacgg aagtattttg tattccgttc
     7441 aagtttatcg cgtaattctg tagattttgt caatatgtca ataaccttaa tcgttgcgcc
     7501 aacaactgat ggggcaacgg tatttgagaa cagataagga cgggctttgt tacgcatcca
     7561 gcttacgatt tccttgcttg aagaaataca tccaccggaa gctccaccta aggcttttcc
     7621 gaaggtggtg gtaataatat caactcttcc catcactcca cgatattcat gagttccgcg
     7681 tccggttttg cccagaaagc ctgtggagtg gctgtcgtca accattacaa gagcatcata
     7741 tttttctgcc aggtcgcaga tctcatctaa tttggcaatg tcgccatcca tcgaaaacga
     7801 tccatcggtg gcaatcatta caaaacggca ccctttttca cgtgcttctt tcaggcagcg
     7861 ttcgagacct ttagccattt tatcgttagc cgggtcaatc tcttccacat cgcgcatatc
     7921 gccgtgtttg tatatccagc gctgagcttt gcacaggcgg ataccgtcga tgatggaagc
     7981 atggttcagt gcgtcggtga taatggcatc gttctcacca agaaggggtt caaaaacggc
     8041 accattagca tcaaaacatg aagaaaacaa aatagtgtct tccatgccaa ggaattcgga
     8101 aaccttgcgt tccagttctt tgtggatgtc ctgtgttccg caaataaaac gtaccgatga
     8161 caatccgtaa ccacggttat caatttcctt atgtgcggct tttataagtt cagggtgtga
     8221 tgaaaggcca aggtaattat tggcacagaa gttcaggcat tttttgc
//
LOCUS       DAOSFP010000062         8265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7401_length_8265_cov_4.582460, whole genome shotgun sequence.
ACCESSION   DAOSFP010000062 DAOSFP010000000
VERSION     DAOSFP010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7401_length_8265_cov_4.582460"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..133)
                     /locus_tag="PLI16_02950"
     tRNA            complement(59..133)
                     /locus_tag="PLI16_02950"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(96..98),aa:Pro,seq:tgg)
     gene            complement(155..246)
                     /locus_tag="PLI16_02955"
     tRNA            complement(155..246)
                     /locus_tag="PLI16_02955"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Ser,seq:gct)
     gene            complement(377..1087)
                     /gene="trmD"
                     /locus_tag="PLI16_02960"
     CDS             complement(377..1087)
                     /gene="trmD"
                     /locus_tag="PLI16_02960"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOH83545.1"
                     /translation="MRIDIITIFPEIFESPFSHSIIKRSKEKGLVTIHLHQLRDYAKN
                     KYKSVDDYPYGGGAGMVMMIEPIAACIEKLKLERTYDEVIYTSPDGELLAQPVANHLS
                     TKENIIILCGHFKGIDERIRERYITREISIGDYVLSGGELAAAIICDSIIRLIPGVLG
                     DETSALSDSFQDGLLAPPVYTRPADFQGMKVPAVLLSGNEAEITKWRYEKSLERTKKR
                     RPGLLNDQNFFSEPTQER"
     gene            complement(1237..4224)
                     /locus_tag="PLI16_02965"
     CDS             complement(1237..4224)
                     /locus_tag="PLI16_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH83546.1"
                     /translation="MKKSATLLVILLFICSFTSAQTKGTRQLLDSAFIYFAMENYHDA
                     LPLFLKLENADPGNANMSYHAGVCYLHMPSEKHNAIPYLKKAADSVSKDYVDNPEGRK
                     APLRAFFDLGTAYLLHYDLDKAIETTEKFKSFLDENVEADKNMRKEADRQIAMCHVAR
                     EMVNHPIPITVENLGKHINTSYPEYSPAIVLDGNTLLFTARRGVNVGDLIEDNGYYFE
                     DVYLSKKSENSEWSEATNIATINTPDHEASISLSPDKKQLFIYKDDGGDGNIYVSNHN
                     EKEWQAPKKLPAPINTKSWETHASLSSDGNTIFFTSDRKGGLGGLDIYKCTKNPDGKW
                     GEAINLGSTINTEYNDDGPFLLYDEILYFCSQGHKTMGGYDIFYSRLKSDSTWGEPVN
                     IGYPINTTDDDLFYTPVDSLTAYFASVRAEDSYGTPGFGDLDIYSLAIHPIMIKGVAY
                     DKNTKKCLPGARINLLNENMELMEVAVTDSNGAFSFPADYNRNYILKVKEDSYEEATN
                     KVSTFDIGDKKEVVADLYPERIFAVSLKIHVVDERTTQPLDGVIVELKDVLAGTSDTL
                     TTDANGDAYKSLGNKKYNDSLHFDLRFTKQGYLAENLEFRHLITKPGEIEIPADLLSE
                     NAQPVSLRIKVLDAKTNEPLKDVKIVLKDKESGTIENLVTDNNGEVTKKLENKKINDS
                     LKYDMVLSKESYVSKDVEYRHLILKPNEILVSETIGKMEVGTDLAKIIQIKNIYFDFD
                     KYYIRPDAKVELDKIVKIMKEYPTIMVELGSHTDCRNTYAYNEALSEKRAQSSVAYIV
                     SKGIDRSRITGMGYGEYQLFTNCPCEGTKESECSEEDHQLNRRTEFRITGFIDGIGQV
                     NIKSEKGTNIHTDPKPGKRLKKSNDAMQQENYQQESDDNAAGQGAGNTTANQDLVYRI
                     QIMACDKDLDTGDPFFKGLEDIYKYWHKGLYKYAWGKSATKEGAEELRIKMVDTGFPD
                     AFIVPFYKNRRITMEEVETLDKK"
     gene            complement(4238..5410)
                     /locus_tag="PLI16_02970"
     CDS             complement(4238..5410)
                     /locus_tag="PLI16_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOH83547.1"
                     /translation="MPKILVIDDEKSIRNTLREILEYEKYQVDDAPDGAEGLQFVDKE
                     KYDVILCDIKMPRMDGIEVLKAIMSKTDTPVVMISGHGNIETAVESIKLGAYDYIAKP
                     LDLNRLLITLRNAMDKSTLMGETKVLKKKISKTWEMIGESPAIKQIKDMIDRVAPTDA
                     RVLITGENGTGKELVARWLHEKSNRAGHPFIEVNCAAIPSELIESQLFGHEKGSFTSA
                     IKQRKGDFEQADGGTIFLDEIGDMSLSAQAKVLRALQENKITRVGGEKDIPVNVRVIA
                     ATNKNMDEEISKKNFREDLYHRISVILIQVPPLNQRLEDIPLLVTHFVKDICGSQGCP
                     LLKVHPSAIEELQKIQWTGNIRELRNVVERLIILCDKEITGQDVKLYAHPLKQNKI"
     gene            complement(5463..7136)
                     /gene="ettA"
                     /locus_tag="PLI16_02975"
     CDS             complement(5463..7136)
                     /gene="ettA"
                     /locus_tag="PLI16_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOH83548.1"
                     /translation="MADDKKIIFSMVNVSKTFPPNKQVLKDIYLSFYYGAKIGVIGLN
                     GSGKSTLLKIIAGIEKSFNGEVVFSPGYSVGMLMQEPQLDDAKTVREIVQEGVQPVVD
                     LLKEYEEVNNKFAEPMSDDEMNKLINRQGELTELLDQHDAWELDNRLERAMDALRCPE
                     SDALVENLSGGERRRVALCRLLLSEPDILLLDEPTNHLDAESVEWLEQHLQQYKGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKTNRLKMEEKTESKRAKTLER
                     ELEWVRMAPKARQAKGKARLKAYESMLNEDVKQKDEKLEIFIPNGPRLGDVVIEAHKV
                     SKAYGDKLLFENLEFMLPPGGIVGVIGPNGAGKTTLFRLMMGLEKPDAGIFKVGETVK
                     IAYVDQAHVDIDPEKNVWEVISEGNENIILGNRTMNSRAYVSRFNFSGPDQGKKAGVL
                     SGGERNRLHLALTLRQEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVIISHDRWF
                     LDRIATHILAFEGDSQVYYFEGTYSEYEENKKKRLGDLAPKRIRYKKLI"
     gene            complement(7376..>8265)
                     /locus_tag="PLI16_02980"
     CDS             complement(7376..>8265)
                     /locus_tag="PLI16_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83549.1"
                     /translation="NCNIEKVFSFSKKSLPMKNFIPAGLLFICLPLVMLAQIKSPDKE
                     QLDAFLKSKTYVVLEDNPFSAFNAFVGDGFSGVWKITPYEVITYEEFDKKMGDSRSSF
                     LFVSQAQMGKNEMFDYSIINLTMGDASKNLNKMPELCIVPVSYAEVDEESYEYRFPVL
                     IKFIDYFISYSQKNQGKDIRQMVDDNAAEMKNYELWLVKNDLAPDVNTLEKIKKYYPY
                     AVKIVTTDEIETAIKNGNPQVAILHKVGPEETQSASSTVYKFIITVKEGKPLYFGDYK
                     TGTGKPDALLEEDFKKMAK"
BASE COUNT         2072 a         1869 c         1765 g         2559 t
ORIGIN      
        1 gcttagaagg ctgttgctct atcccctgag ctacgggacc ttttccggat cttgtatgtc
       61 ggggtagcag gattcgaacc tgcgaccccc tggtcccaaa ccaggtgcgc tagccgggct
      121 gcgctatacc ccgaaaatca tcaaaaaaac atagtggcgg agagaggggg attcgaaccc
      181 ccggtaccag ttacccagta cgacagttta gcaaactgtt ggtttcagcc actcacccat
      241 ctctccaaag atgtttataa tgttgaatta gacaatcaaa aataaaataa agaacatttc
      301 ccatatcaaa aacgggagtg caaaagtatg aaaatgtttt atattgtcaa aaaataattt
      361 ttcaataatt aaaatttcaa cgctcctgtg ttggctcaga gaaaaaattc tgatcgttca
      421 aaaggccggg gcgtcgtttt ttggtacgtt cgagggattt ttcataacgc cattttgtta
      481 tctcggcctc attgcccgaa agcaacacgg cagggacttt catcccctga aaatcggccg
      541 gtctggtata taccggcgga gccagcaagc catcctgaaa agaatcggac aaggcagatg
      601 tttcgtcgcc cagcacaccg ggtataagcc gtataatact atcgcaaatg attgccgcag
      661 cgagttcgcc gcccgacagc acataatctc caatggaaat ttcgcgggta atataccgtt
      721 cgcggatacg ttcatctatc cctttgaaat gtccacacaa aataatgatg ttttcctttg
      781 ttgacaaatg gttggccacg ggttgtgcaa gcagttcgcc gtcgggagag gtgtagataa
      841 cctcgtcgta agtacgttct aattttaatt tttcaatgca ggcagcaata ggctctatca
      901 tcatcaccat accggcaccg cctccatagg ggtagtcgtc cacgctttta tatttattct
      961 tcgcatagtc gcgcaactga tgaagatgta tagtaaccag gcctttttct ttggatcttt
     1021 taatgatgga atggctgaaa gggctttcga aaatttcagg gaaaatggtg ataatatcga
     1081 ttcgcatctt ggtgcttttt ttacaaaaat atccaaaaat tattttaaag ccttcccaat
     1141 caaacaaaac aaaaagtgac cgtatataaa tcaatgctta ctgcataatc atcatctatc
     1201 ccgcatgcgg cagtataaaa ttatgcacag cggtaactat tttttatcaa gcgtttccac
     1261 ttcttccatg gtaattcttc tgtttttata aaagggaaca ataaaggcat ccgggaagcc
     1321 agtatccacc atcttaatac gaagttcctc tgccccttcc ttcgtcgcag attttcccca
     1381 ggcatatttg tacaatccct tatgccagta tttataaata tcctccaatc ctttaaagaa
     1441 aggatctccg gtgtctaaat ccttatcgca ggccattatt tgtattctgt acaccaaatc
     1501 ctggttagca gtggtattgc cggcaccctg gccggcagca ttatcgtcgg attcctgctg
     1561 ataattttcc tgttgcatgg catcattact ttttttcaat cgttttcccg gtttgggatc
     1621 ggtatggatg ttggtacctt tttcgctttt gatatttacc tggccgatac catcgatgaa
     1681 tccggtgatt ctgaattcgg tgcggcggtt gagctggtga tcttcttcgg aacactccga
     1741 ttccttcgta ccctcacagg gacagttggt aaacagctgg tattcgccat aacccatgcc
     1801 ggtaattctt gagcggtcaa ttcccttgct cacgatatag gcgacactgg actgggcgcg
     1861 tttttccgac agggcttcat tataagcgta cgtgttgcgg cagtcggtat gtgagccaag
     1921 ttccaccata attgtcggat actctttcat gattttaacg atcttatcca attctacttt
     1981 ggcatcggga cgaatataat atttgtcgaa gtcgaaatag atatttttta tctgaatgat
     2041 tttagccagg tcagtaccaa cttccatttt tccaatggtt tcgctgacca atatctcgtt
     2101 gggttttaaa ataaggtgtc tgtattcaac atctttcgaa acatagcttt ctttgctaag
     2161 caccatatca tatttcaggc tgtcgttgat ttttttgttt tcgagctttt tggtcacttc
     2221 tccgttattg tctgtaacca ggttttcgat agtgccagac tccttgtctt taagcacaat
     2281 ttttacatct ttcagcggtt cgttggtttt ggcgtccagt acttttattc tcagcgacac
     2341 gggctgtgca ttttctgaca acaggtcggc aggtatttca atttctcccg gcttggtgat
     2401 caggtgccga aactcaagat tttccgccag atagccttgt ttggtaaacc gcaggtcgaa
     2461 gtgcaggctg tcgttatatt ttttgtttcc aagtgattta taggcatcgc cattagcatc
     2521 tgtggtaagt gtatcggagg tacctgcaag cacgtctttc aattccacga taacgccatc
     2581 gagcggttgg gttgttcttt catcaacaac atgtatcttc agactaacag cgaatatcct
     2641 ttcggggtac aggtcggcca caacttcttt tttatcgccg atatcaaagg ttgaaacctt
     2701 gttggtggcc tcttcatagg aatcttcctt tacctttagg atgtaattcc ggttatagtc
     2761 ggcagggaaa gaaaaagcgc cgttagagtc ggtaacggcc acttccatta attccatatt
     2821 ctcgttcaac aggtttatac gtgctcccgg caagcatttt ttagtgtttt tatcataggc
     2881 cacacctttg atcatgattg gatgaatggc aagactataa atatccaggt cgccaaagcc
     2941 gggggttccg tagctgtcct cggcacgaac cgaagcaaaa taggcagtga gcgaatccac
     3001 cggggtgtaa aacaggtcgt cgtcagtggt gtttatagga tagccaatat ttacaggttc
     3061 gccccaggta gagtcacttt tcaggcggct gtaaaaaata tcgtagcctc ccatagtttt
     3121 atgtccctga gaacagaagt acaatatttc atcatagagc aaaaaaggtc cgtcatcgtt
     3181 atattcggta ttaatcgtgc tgccgaggtt tatggcctcg ccccattttc catcaggatt
     3241 ttttgtgcat ttatagatat ccagtccgcc caaaccgcct ttccggtcgc tggtgaaaaa
     3301 tatggtattc ccatcagacg acaggctggc atgtgtttcc cacgatttgg tgttaatggg
     3361 cgccggaagt tttttgggcg cttgccattc cttttcatta tgattactga cataaatatt
     3421 tccatcgcca ccatcgtcct tgtaaataaa taattgtttt ttatcaggcg ataggctgat
     3481 agaggcttca tggtcgggag tattgatggt tgctatgttg gtggcttctg accattcaga
     3541 gttttcactt tttttcgaca aatacacatc ttcaaaataa tacccgttat cttcgattaa
     3601 atcgcccaca ttcacgcccc tgcgtgctgt gaacagcagg gtgttgccat cgagcacaat
     3661 agccggcgaa tattcggggt agctggtatt gatatgcttt cccaaatttt caaccgtgat
     3721 aggtatcgga tggttcacca tctcacgagc cacatgacac atagctatct ggcggtcggc
     3781 ttcttttctc atatttttat ccgcttccac attttcatcc aggaatgatt taaatttttc
     3841 agtggtttct atagctttgt ccaagtcata atggagcaga taggctgttc ccagatcaaa
     3901 aaaagcacgc aagggagctt ttcggccttc gggattatcc acatagtctt tggaaacgct
     3961 gtcggcagct tttttcaggt atggaattgc attatgtttt tcggaaggca tatgaagata
     4021 gcagacacct gcatgataag acatattggc attccccgga tcagcgtttt caagttttaa
     4081 aaacagcggt aatgcatcat gataattttc catggcaaag taaataaatg ccgaatcgag
     4141 caattgccgg gtaccttttg tttgagcaga tgtaaaggaa cagataaaca ataatattac
     4201 gagcaatgta gcgctttttt tcatagtgtt agtgtattta tattttgttt tgttttaaag
     4261 gatgggcata aagtttcaca tcctgtcctg taatttcttt atcacaaagt attatcaagc
     4321 gttctacgac gtttctgagt tcacggatat tgccggtcca ttgtattttc tgcagttctt
     4381 cgatggctga gggatgaact tttaacagcg gacagccctg gctgccgcaa atatctttga
     4441 caaagtgggt taccagcaaa ggaatatctt ccagccgctg gttgagaggc ggtacctgta
     4501 tgagtataac gctgatacga tggtagaggt cttcacgaaa attctttttg cttatctcct
     4561 cgtccatatt tttgtttgtg gcagcgataa ccctgacatt gacagggata tctttttcgc
     4621 ctcccacacg ggtaattttg ttttcctgca gggcgcgcaa cacctttgcc tgggcagaca
     4681 ggctcatgtc gccaatttcg tcaagaaaaa tagtaccacc gtcggcctgt tcaaaatcgc
     4741 ccttgcgctg ttttattgca gaggtaaaag atcctttttc gtggccgaaa agttggcttt
     4801 ctatcagttc ggagggaata gcggcacaat tcacttcgat aaaaggatgg ccggcgcggt
     4861 tgcttttttc gtgcaaccag cgggccacca attcttttcc cgtaccattt tcgccggtta
     4921 tgagcacgcg ggcatccgtt ggggctacgc ggtcaatcat atctttgatc tgtttgatgg
     4981 ccggcgattc cccaatcatc tcccaggttt tgctgatctt ctttttcagc actttggttt
     5041 cacccatcag ggtggacttg tccatggcat tacgcagggt aatcagcagg cggttcaggt
     5101 cgagcggctt ggcgatataa tcataagcgc ccaattttat tgattccacg gctgtttcga
     5161 tattgccatg gcctgatatc atcaccaccg gagtgtccgt tttactcata atggccttga
     5221 gcacctcaat accgtccatt ctcggcattt ttatatcaca taggatgaca tcatattttt
     5281 ctttgtcaac aaattgcaag ccttcggcac catcaggagc atcgtcaacc tgatattttt
     5341 catattcgag gatttcccgc agtgtgttgc ggatactttt ttcatcgtcg attactagta
     5401 ttttgggcat ttggagaagg ggtttagact gttaggggtt acgctgttag aaggttgtgg
     5461 ggttatatta gttttttgta acggattctt ttgggggcca ggtctcctaa tcgttttttc
     5521 ttattttctt catattccga ataggtgcct tcaaaataat atacctgtga atcgccttca
     5581 aaagcaagga tatgtgtggc aatacggtcg aggaaccagc ggtcgtggga gatgatgacg
     5641 gcacatccgg caaaattttc gaggccttct tccagggcgc ggagggtgtt gacatcaata
     5701 tcgttggtgg gttcatcgag cagcagcaca ttggcttcct ggcgcagtgt aagagccaaa
     5761 tggagccggt tgcgttctcc gcctgacagc actccggcct tttttccctg gtcggggcca
     5821 ctgaaattga aacgggagac ataagccctt gagttcatgg tacgatttcc gagaataata
     5881 ttttcattgc cttcggagat gacttcccat acattttttt cggggtcaat atccacatgg
     5941 gcctgatcta cataagcaat ttttactgtc tccccaactt taaaaattcc ggcatcgggt
     6001 ttttccagtc ccatcatcag gcggaacagg gtggttttgc cagcaccgtt agggccaata
     6061 acgccgacaa tacctcccgg aggaagcata aattcaaggt tttcaaataa taatttatcg
     6121 ccgtaggctt tggacacctt atgggcttcg ataaccacat cgcccaggcg ggggccgtta
     6181 gggataaata tttcgagttt ttcgtctttc tgtttaacat cctcgttcag catgctttcg
     6241 taggctttga gcctggcttt gccttttgcc tggcgggctt tgggagccat acgcacccat
     6301 tccagctcac gttcgagggt tttggcacgt ttactctcgg tcttttcctc catcttcagc
     6361 ctgttggttt tctgctccag ccacgaagag tagttgcctt tccaggggat accttctccc
     6421 ctgtcgagct cgagtatcca accagccacg ttatcgagaa aatagcggtc gtgggttaca
     6481 gcgataacag tccctttgta ctgctgcaga tgttgttcca gccactccac cgactcggca
     6541 tcgaggtggt tggtaggttc atcgagcaac aaaatatccg gctctgagag caacaatcgg
     6601 cacaaggcca cacgacggcg ttcgccaccc gaaaggtttt caacaagggc atcgctttca
     6661 ggacacctca gggcatccat ggcgcgttcc aatctgttgt caagttccca ggcatcatgc
     6721 tgatcgagca attccgtaag ttctccctga cggttgatga gcttgttcat ctcgtcatcc
     6781 gacataggtt cggcaaattt attgttgact tcttcgtatt ctttaagcaa atctacaaca
     6841 ggctgcaccc cttcctgcac aatctccctg acggttttgg catcatcgag ctggggttcc
     6901 tgcatcagca tgcccaccga ataaccgggt gagaaaacca cttccccgtt gaaagatttt
     6961 tcgatgcctg caattatctt caacaatgtt gatttcccgg aaccgttaag ccctattaca
     7021 cctatcttgg caccatagta aaaggacagg taaatatctt tcagtacctg tttgtttggt
     7081 ggaaacgttt tgctgacgtt caccatggaa aaaatgattt ttttgtcgtc tgccatgtaa
     7141 taataagagt ttatacgctt tttcttttaa aaagttcgca ttttgtgtaa aacaaaaata
     7201 caaattatta ccagacctaa taacctgtgc gcaaataaga ttaagattat taaaaaaatt
     7261 attaggttac aacaatcaaa gccctgatta tcatttacaa atcaaaatat aagaatatct
     7321 gccttgatta tttttctgaa ataaaagtca gtaatattac ctattcttgg gatttttact
     7381 tcgccatctt tttaaaatcc tcttccagca aggcatcagg tttgccggtt ccggtcttat
     7441 aatcgccgaa atacaggggt ttgccttctt ttaccgtgat gataaactta taaaccgtac
     7501 tgctcgcgct ttgtgtttct tcaggcccta ccttatggag aattgccacc tgtggattcc
     7561 catttttgat ggcggtttca atttcatcag tggtaacaat tttcacagca tagggataat
     7621 attttttaat cttctccaaa gtattgacat cgggggcaag gtcgtttttt accagccata
     7681 gttcataatt tttcatttcc gccgcattat catccaccat ctgccgtata tccttaccct
     7741 gatttttttg tgagtaactg ataaaataat caatgaattt tatcagcacc gggaacctgt
     7801 attcataaga ttcttcgtcc acctcggcat aggaaacggg cacaatacac agctcaggca
     7861 tcttgtttag gtttttggaa gcatcgccca tggtaaggtt aataattgaa taatcaaaca
     7921 tttcattttt tcccatctgg gcctgcgaca caaagaggaa cgagctgcgg ctgtctccca
     7981 ttttcttatc aaattcttca taggtaatca cttcgtatgg agtaatcttc caaactccgc
     8041 taaacccatc gccaacaaag gcattgaatg ccgaaaacgg gttatcctca agaaccacat
     8101 aagttttgct tttcaggaat gcatccaact gttctttgtc gggggattta atctgtgcca
     8161 acatgacaag aggcaggcaa atgaacaata ggcctgccgg aataaaattt ttcatgggta
     8221 aacttttttt tgaaaacgaa aaaaccttct caatattaca atttc
//
LOCUS       DAOSFP010000063         8249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7423_length_8249_cov_3.760312, whole genome shotgun sequence.
ACCESSION   DAOSFP010000063 DAOSFP010000000
VERSION     DAOSFP010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7423_length_8249_cov_3.760312"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="PLI16_02985"
     CDS             complement(<1..1690)
                     /locus_tag="PLI16_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83550.1"
                     /translation="MKICNYFFLLIMLVGSICLSAQTSVIKGTVSGLTAASDQLKKEK
                     LPFATIYWINRGTSTTADKNGKFSIQFDGKEASKLVASFVGYKSDTILIRQGQNDIDF
                     VLISNATLEAVEIKEKVDDYVSKLKPIKTEIITETGLQKLACCNLSESFERTATVDVG
                     YADAVSGARRIQMLGLSGIYSQLLTENFPAVRGLTSSYGLTYIPGAWMHSIQVSKGTS
                     SVTNGYESITGQINVEYKKPETNKEPLFLNVYGNSDGRFEANLNSTIKINDRWSTMLL
                     GHGSYFGFMTDKNKDSFLDTPKSWTANFMNRWQYEVEGKMHVQFGIGYLQDNKQGGQS
                     GYYGSDEQQKPFFYGIGMDAKQYSAFVKTGFFVGDHGHSSLGIIGSFNRYEMNSFYGM
                     NTYDGIQNSGYFNLIYQSIILNTNHKFSTGGSFMLDDYRETLNDSVFKRTEYVPGLFF
                     QYSYTYPEKLNVIVGLRGDYNSQYGIFFTPRVHVKYDITDKLTLRASAGRGYRSPNII
                     AENTSLLASSRKIVLESDIKAEEAWNYGLNITKEFLLTETLKASLGADFYHTAFV"
     gene            complement(1769..2239)
                     /locus_tag="PLI16_02990"
     CDS             complement(1769..2239)
                     /locus_tag="PLI16_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83551.1"
                     /translation="MKKFFFKSILFVSLVAFLMASNGVFYINHYCFKSEKNSTSFVRY
                     YEDNCCESIDKCCVKDESHHRGVCCANRHNERTPDVSIGNEKCCKDQNYYLKVYSSYL
                     LPDNSLLDFQSFLYACEIQEQIEITPYSHGIMSCNSPPPELNIPVFLKNRILRL"
     gene            2470..2841
                     /gene="rplS"
                     /locus_tag="PLI16_02995"
     CDS             2470..2841
                     /gene="rplS"
                     /locus_tag="PLI16_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOH83552.1"
                     /translation="MNKTEIMNFVEEQFSKKPEYPAFKAGDTITVSYKIKEGNKERIQ
                     QFQGVVIQRSGEGLKETFTVRKMSGNVGVERIFPIASPFIDKIDVNKKGMVRRARIFY
                     LRDLTGKKARIKEKKKSQSDK"
     gene            complement(2962..4245)
                     /locus_tag="PLI16_03000"
     CDS             complement(2962..4245)
                     /locus_tag="PLI16_03000"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOH83553.1"
                     /translation="MKQLTAVLLLIICMVASGQQINYNYLPVKVNGKYGFVDTAGKYL
                     IRPDYDFLGCLVEGRARVNVGGKPDDHGFMEGGFWTFMDSLEQPVSAPVYEAVSDFCE
                     GRARVMKHGLYGFIDKNGLEVIPCQYPNITDFHNGMAQFEKDGLWGSIDTDGNMVISP
                     RFDKVYYFNEDRAVVGHPFYKAYINKLGNFITDSIFFEAKSFENGLAIVVSGDSVRYG
                     MLDAFGAKVIPCIYGYITRFSEGLAAVNLNGVFVAEKNEVTGGHWGFIDKGNRLVIPA
                     DYDNAGAFSENLCPVKKNEKWGYIDKDNKLRISFKYQMAYLFSCDRARIVKKSTTGFI
                     DNSGTVIIKPGFEEAEDFSSGLAIVRQGSAGNKKNRQPGQTGKYGIINKAGEFVLYPE
                     FDAITRFGNKTFRVYKGSGWGYYNTKGECVFSISE"
     gene            complement(4245..5489)
                     /locus_tag="PLI16_03005"
     CDS             complement(4245..5489)
                     /locus_tag="PLI16_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH83554.1"
                     /translation="MYYDVIVVGGGASGLMCAGIVAGRGLSVLLLEKMPLPARKLRIS
                     GKGRCNLTNIASKERFMEHIGPDARFLHNAFGAFFAQDLIDFFESLGVKNVVEQGGRV
                     FPESGKATDVVDALTAWAKSSGAVLKCSAPVRELIIENNMVTGVETTTHEHYAAGAVV
                     VATGGLSYPATGSTGDGYRFARLTGHTVFPLRPMLVPLTSHDAFVQELNGLHLKNIGI
                     AVKVNDSKKHYITGEMEFRDNTLAGPIILSLSRQFIDDIRAGKKVVFCFDFKPGLDYE
                     KLDARLLRDLDALGKAGCVELLKGLLPGQLIPVFKKILAIAGDKKSSQITAAERKKIA
                     ALLKEFPVNIAAHRDYNEAIITGGGVSTKEVNPKTMESRKIKNLFFTGEVLDLDADTG
                     GYNLQIAFSTGYLAGISIQKKL"
     gene            complement(5542..>8249)
                     /locus_tag="PLI16_03010"
     CDS             complement(5542..>8249)
                     /locus_tag="PLI16_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOH83555.1"
                     /translation="TDSVQADLFRLHESGRGNGIFTYNMQQQTNYNQAVDFTDSDNYW
                     NNVNANLDEAATDAHWGAEMTYDYYWDTFGRNSIDGDGFALYNYVHYDQAYGNAYWNG
                     DCMTYGDGENNNPFCALDVCAHEITHGLDEFTANLDYVDESGALNEGFSDIFGTAVEY
                     FAKPAQANWTCGENIGFIIRNLQNPNAEGDPDTYLGLNWDPAQEVHRNSTVLSHWYYL
                     LCQGGSGTNDNNDVYSVTGIGMNKASDIAFRMLTVYLINTSDYQDARFFAILAASELY
                     GYCSTEVASVTNAMYAVGLGDAYTAHPAIQFFASNAQVCSVPLTVNFENQTINANHYM
                     WYFGDGATDTTANPSHTYTSFGNFDVKLVATGAACGIDSLTQTGLVSIDAANSNYASM
                     PVTGNGQALTCCTGTLFDSGGLGDYSNNTNGTVTIAPTGASNVTLNFTSFDFESGYDY
                     LYIYDGPTTASTLIGQYDGTNLPNGGTIQSSTGQITLRQTTDQGLTTPGFELTWHCSI
                     PSTPPVTNFMASETTSCTGVIQFLDLSFNGPVTWHWDFGDGDTSNQQNPLHAYQAEGT
                     YTVTLTTTNNFGDSTKTLADYIVVQNLPAVPSVTPGAACDSSSVILTASGTGQINWYD
                     METGGTLLYVGDTFQTPVLYQNTTFYVENALVNPDEYVGKTSKSNDAGLHNNNNYFLI
                     FDCYTPMILKSVKVYAGDEKYRIIELRDSNGIVLQTINALVPAGESRVTLNFNLPVKN
                     NMSLACGTDDPNMYRDQSGITFPYNLAGKISIKGTNAAPNVRYYYYYDWEVQEPPCIS
                     PRVPVLASVYACNGLMEPENINNSIVYPNPANGKFFVEFTSTGQDDYIITVHDVIGKE
                     VYSQQSTSVNGRNSHAIDCSSFNSGIYVISLTAGNYNYRQKIIVH"
BASE COUNT         2365 a         1876 c         1684 g         2324 t
ORIGIN      
        1 taacaaaagc cgtatgataa aaatcagctc cgagactggc tttgagtgtt tccgtcagca
       61 aaaactcttt ggtaatattc aatccgtaat tccatgcctc ttcggctttg atatcgcttt
      121 ccagaacaat ttttcttgaa ctcgccagca gacttgtgtt ttcggcaata atgttgggtg
      181 aacggtatcc cctgcctgct gaggcacgaa gcgttaattt gtcggtaatg tcatatttga
      241 catgtactct tggtgtgaaa aaaattccgt attgagagtt ataatctccg cgcaaaccaa
      301 caatcacgtt tagtttttca ggataggtat aggaatactg gaaaaacagg cccggaacat
      361 attcggtcct cttaaaaaca gaatcattga gggtttcacg ataatcatcc agcataaaag
      421 agcccccggt gctgaatttg tgattggtat ttaagatgat tgactgatat atcagattaa
      481 agtaaccgct gttttgaatt ccgtcataag tattcatgcc ataaaaggaa ttcatttcat
      541 accggttaaa agagccaatg atacccagac tggaatgtcc atggtcacca acaaaaaaac
      601 ctgtttttac gaaggcgctg tattgctttg catccatacc gataccataa aagaatggtt
      661 tttgctgttc atcggaacca taatacccgg actgtcctcc ctgtttgtta tcctgcaggt
      721 atcctatgcc aaactgcaca tgcattttgc cttccacttc atattgccag cggttcatga
      781 aatttgctgt ccacgatttg ggtgtatcca gaaaagagtc tttgttttta tcggtcataa
      841 agccgaaata agagccgtgt ccgagcagca tggtgctcca cctgtcatta atcttaatgg
      901 tagagttgag gttggcttca aaacggccat cgctgtttcc ataaacattt aaaaacaaag
      961 gttccttgtt ggtttcaggc ttcttatact ccacgttaat ctgtcccgtg atggattcat
     1021 agccgttagt aaccgatgag gtccctttag aaacctggat ggaatgcatc catgcacctg
     1081 gaatataagt cagaccgtaa gaagaggtca atcccctgac agccggaaag ttttcggtaa
     1141 gtaactggct gtaaataccc gacaaaccca gcatctgaat acgtctggcg cctgacacgg
     1201 catcagcata gcccacatcc acggtagcgg tacgttcgaa actttcggaa aggttgcaac
     1261 aggcaagttt ctgaaggcct gtttctgtaa tgatttccgt ctttatgggt tttagttttg
     1321 aaacataatc atccactttt tcttttattt ccacagcttc gagcgtggca ttggatatca
     1381 ggacaaagtc aatatcattc tggccctggc ggataagtat ggtatcgctt ttatagccca
     1441 caaagctcgc aacgagtttc gaggcttctt ttccatcaaa ttgtatggaa aactttccat
     1501 ttttgtcggc ggtggttgaa gtgccgcggt taatccaata aatggttgca aaagggagtt
     1561 tttctttttt cagttgatca gaagctgcgg ttagtccgct cacagtacct ttaattactg
     1621 atgtctgagc cgacaaacat atactgccaa caagcattat cagcagaaaa aaataattac
     1681 aaattttcat aaataaattt ttattacaat taatacaaat caattccaat aagactttta
     1741 gccttaggtg acgaaattgt aataaaaact ataaccttag aatcctattt tttagaaaaa
     1801 ccggtatgtt cagttccggt ggcgggctgt tacaggacat gatcccgtgt gaatagggag
     1861 taatctctat ttgctcctga atttcacatg cgtacaaaaa actttgaaaa tccaacagac
     1921 tattatccgg caagaggtaa gaactatata ctttcagata ataattttga tccttacaac
     1981 acttttcatt cccgatgctc acatcgggtg ttcgttcatt gtgtctgttg gcgcaacaca
     2041 cacccctgtg atgactttcg tctttaacgc agcatttatc aatactttcg caacaattat
     2101 cctcgtaata cctgacaaaa ctggtgctgt ttttttctga cttaaagcaa taatggttaa
     2161 tataaaacac accatttgaa gccatcagaa aggccactaa cgaaacaaac aatatgctct
     2221 tgaaaaaaaa ttttttcatt ggagtgcaaa gataatttaa aatcattcta attaaaacaa
     2281 tcacgatcga tttaacaagt atttaactgc acaaatgcct tttgacaaca atatccagtc
     2341 ttctgattta tttgcaaaac aaacgggaaa catataattg tgcgctaaat ttaaaaaaat
     2401 ttgccgtgaa attagaaagt atgaaaaatt aatgtatctt tgcactccaa tttctaaaaa
     2461 aacacagtga tgaacaagac ggagataatg aattttgttg aagagcaatt cagcaaaaag
     2521 cccgaatatc ctgcattcaa agccggagat actattacag taagttataa aatcaaagaa
     2581 ggaaacaagg aacgtattca gcagtttcaa ggcgttgtaa ttcagcgttc aggcgaaggc
     2641 ttaaaagaaa ccttcactgt aagaaaaatg tcaggcaacg taggtgtaga aagaattttc
     2701 cccattgctt caccttttat tgacaaaatt gatgtcaaca aaaagggtat ggtacgcagg
     2761 gcacgcattt tctacctaag agaccttacc ggtaagaaag cccgtatcaa agaaaaaaag
     2821 aaaagtcagt cagacaaata atattgaaac cccgcaaatg cggggttttt tgtttcacaa
     2881 aaattcaata cggcctgtcg tttggccgat cagattggcg ttcagacaaa caatattata
     2941 gttcggaata taataataag gttattcgga aatcgaaaaa acacactcgc ccttggtatt
     3001 atagtacccc caaccacttc ctttatacac cctgaaggtt ttgttcccaa aacgggtaat
     3061 tgcatcaaat tcgggataca gaacaaactc ccctgccttg tttattatac cgtatttgcc
     3121 cgtttggccg ggctgccggt tttttttgtt tcccgcactc ccctgcctga caatggcaag
     3181 gccgctgctg aaatcttccg cctcttcaaa cccgggtttt ataatgacag tgcctgaatt
     3241 atcgataaaa ccagtagtac ttttttttac aatgcgcgcc ctgtcgcagg aaaacagata
     3301 tgccatctga tacttaaaag agatacgcag tttattgtct ttatcaatat aaccccattt
     3361 ttcatttttt ttgaccgggc ataaattttc ggaaaaagca ccagcattat cataatcagc
     3421 cggaatcacc agcctgttgc ctttatctat aaatccccaa tggccacctg ttacctcatt
     3481 cttttccgcc acaaacacac cgttcagatt cacggcagcc aggccttcac taaagcgggt
     3541 gatatatcca taaatacagg gtattacttt tgcgccgaaa gcatccaaca tgccatagcg
     3601 gaccgaatcg cccgacacca caatggcaag gccattttca aaacttttcg cttcaaaaaa
     3661 aatggaatca gttataaagt ttcccaattt gttgatatat gccttgtaaa acggatggcc
     3721 aaccaccgca cggtcttcgt taaaataata aactttatcg aaacggggcg atatcaccat
     3781 attgccgtct gtatcgatgc ttccccaaag cccgtccttt tcgaattgtg ccattccatt
     3841 atgaaaatct gtaatattcg gatattgaca tggaataact tccagcccgt ttttatcaat
     3901 aaagccatac agcccatgct tcataaccct tgcccggcct tcacaaaaat cactgacagc
     3961 ttcataaaca ggtgcggaca ccggctgttc caatgaatcc atgaaagtcc aaaaaccacc
     4021 ttccatgaag ccatgatcat cgggtttgcc gcccacattc acccgggccc tgccctcgac
     4081 caggcacccc aaaaaatcat aatcaggcct tataaggtac ttacctgcag tatccacaaa
     4141 tccatatttc ccgttgactt ttacggggag gtaattgtag tttatctgtt ggccgctggc
     4201 aaccatgcaa atgataagta acagcacagc ggtcagttgc ttcattataa ttttttttga
     4261 attgaaatcc ccgccagata gcctgttgaa aaagcaatct gaagattata tccgccggtg
     4321 tcggcatcca aatcaagcac ttcgccggta aaaaacaaat tttttatttt tcgggactcc
     4381 atggttttgg ggtttacctc tttggtagaa acgccgcctc ctgtgatgat agcctcgtta
     4441 taatcacgat gtgcggcaat attcacagga aactctttca acaaagcagc aatttttttt
     4501 cgttcagccg cagtaatctg gctgcttttc ttatctccgg caatcgccag tatttttttg
     4561 aataccggta tcaattggcc gggcagcagg cctttgagca attccacgca accggctttg
     4621 ccaagagcat ccaggtcgcg cagcaaacga gcatccagtt tttcgtaatc caaacccggt
     4681 ttaaaatcaa aacaaaacac tacctttttt ccggccctga tatcatcaat aaattgcctg
     4741 cttagagaaa gaataatggg ccctgcaagc gtgttatccc tgaattccat ttccccggtt
     4801 atgtaatgtt ttttactatc atttaccttc acggcgatac cgatattttt aagatgcaac
     4861 ccatttaatt cctgcacaaa agcatcatgt gacgtcagtg gcaccagcat aggacgaagt
     4921 ggaaacacgg tgtggcctgt cagacgggca aaacgataac catcgcccgt agaacctgtt
     4981 gcagggtatg aaaggccccc ggtagccacg accacagctc cggctgcata gtgttcatgg
     5041 gtagtggttt ctacgccggt aaccatatta ttttcaataa tcagttcacg gacaggggcc
     5101 gaacatttga gtaccgcacc cgagcttttt gcccaggctg tcagtgcatc taccacatcg
     5161 gtagcctttc cgctttcggg aaaaactctt ccgccttgtt ctaccacatt tttcacacct
     5221 aaactttcaa aaaaatcgat gagatcctgt gcaaaaaacg ccccgaaagc attatgcaaa
     5281 aaacgggcat ccggcccaat atgctccata aagcgttctt ttgatgcgat attagtcagg
     5341 ttgcaacggc cttttccgct gatacgcaac ttgcgggcag gtaacggcat tttctccagc
     5401 aaaagtactg acaaaccacg ccctgcaaca attccggcgc acatcaaccc ggaagctcct
     5461 cctccgacaa caattacatc ataatacatt gacgctgatt tttcaaagct atttgttgca
     5521 ggacattttt gaaagggctt ctcagtgaac aataattttt tgcctgtaat tataatttcc
     5581 ggcagttaaa ctaataacat aaatgccgct gttaaaagag ctgcaatcta ttgcatgtga
     5641 attacgcccg ttaacggaag tactttgttg cgaataaacc tctttcccga tcacatcgtg
     5701 tacggtaata atataatcat cctgcccggt gctggtaaat tccacgaaga atttgccatt
     5761 agcaggattg gggtacacaa tactgttatt gatattttcc ggttccatga ggccattgca
     5821 tgcgtacacc gaggcaagta cgggtacccg gggacttatg cagggtggtt cctgcacttc
     5881 ccagtcataa tagtagtaat agcggacgtt gggagcagca ttggttccct ttatggaaat
     5941 tttgccggca agattataag ggaaagtaat tccactctga tcgcggtaca tattgggatc
     6001 atccgtacca caggcaaggc tcatattatt ttttacaggc aaattaaaat tgagcgttac
     6061 cctgctctcg ccggctggta ccaatgcgtt gatggtttgc aacactatcc cgttgctgtc
     6121 tctgagttcg ataattctgt atttctcatc gccggcatac actttcacgg attttagaat
     6181 cattggcgta taacagtcaa atataaggaa atagttgttg ttgttgtgaa gtccggcatc
     6241 gttggatttg ctggtttttc ccacatactc gtcgggatta actaaagcat tttccacata
     6301 aaaagtagta ttttggtaca acaccggggt ttgaaatgta tcgccgacat acaggagggt
     6361 tcctccggtt tccatatcgt accagttgat ttgtccggtg ccggaagctg taagaataac
     6421 actggaggag tcgcaggctg cacccggtgt tacggaaggc accgcgggca ggttctgaac
     6481 aacaatgtaa tctgccaggg tttttgtact gtcaccaaaa ttatttgttg tagtaagtgt
     6541 taccgtatag gttccttcgg cctgataggc gtgcagggga ttttgctgat tcgaggtgtc
     6601 gccgtcgcca aagtcccagt gccaggtaac aggcccgtta aaacttaaat ccagaaattg
     6661 aattactccg gtacagctag tagtttcaga agccatgaaa ttggttaccg gcggtgtgct
     6721 tggaatactg caatgccagg tcaattcaaa tcccggtgtg gtaagtccct ggtctgtggt
     6781 ctgccggagg gttatttgtc cggtggatga ctgaatagtt ccgccgttgg gcaggttggt
     6841 cccgtcatat tgtccgatca atgttgatgc tgttgtaggg ccatcgtaaa tatagaggta
     6901 atcatagccc gattcaaaat caaaagaggt aaaattcagt gtaacattgg aagcaccggt
     6961 aggcgcaata gttacagtac cattggtatt attggaataa tcgcccaggc cgccgctgtc
     7021 aaacaaagta ccggtacagc aggtaagtgc ttgtccattt ccggtgacag gcatcgaagc
     7081 ataattggaa tttgcggcgt caatactaac caatcccgtt tgtgtcagcg aatcaatacc
     7141 gcaggcagct cctgtagcca ccagttttac gtcaaaatta ccaaaagaag tataagtatg
     7201 agaaggattg gcagtggtat ccgtagcgcc gtcaccaaaa taccacatgt agtggttcgc
     7261 gtttattgtc tgattttcaa aattaaccgt caatggcaca gagcaaacct gtgcgttgga
     7321 tgcaaaaaac tgtatcgcag gatgtgccgt ataggcatcg cccaaaccca cagcatacat
     7381 agcattagta accgacgcca cttcggtgct acagtatccg taaagttcag aggccgccag
     7441 aatagcaaaa aaacgggcat cctggtagtc tgaagtgttt atcaggtaaa cggtcaacat
     7501 tctgaaagca atatccgaag ctttgttcat gccgataccg gttaccgagt aaacatcgtt
     7561 attgtcgttg gtgccgcttc ctccctgaca aagcaggtaa taccaatggc tcagcacggt
     7621 gctgttccgg tgaacctcct gtgccggatc ccagttcaat cccaggtagg tatcgggatc
     7681 tccttcggca ttgggatttt gaaggttacg gattataaaa cctatgtttt cgccacatgt
     7741 ccagtttgcc tgagccggtt tggcaaagta ctctactgcc gtaccaaaaa tatcactgaa
     7801 cccttcattg agtgcccccg attcgtcaac ataatcgagg ttggcagtaa actcgtcgag
     7861 tccgtgagta atctcatgag cacacacatc caaagcgcaa aaaggattgt tattttcgcc
     7921 atcgccatag gtcatacaat cgccgttcca gtatgcatta ccataagcct gatcgtagtg
     7981 tacatagttg tataaggcaa agccatcgcc atcaatactg ttacggccaa aggtgtccca
     8041 gtaataatca taagtcatct cagcgcccca gtgagcatcg gtggccgcct catccaggtt
     8101 ggcgttcaca ttgttccagt aattgtcaga atccgtaaaa tctacagcct gattgtagtt
     8161 ggtttgttgc tgcatattgt aagtgaatat cccgtttcct ctcccgcttt cgtgcaaacg
     8221 aaaaaggtca gcttgcaccg aatcggtga
//
LOCUS       DAOSFP010000064         8248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7424_length_8248_cov_4.010619, whole genome shotgun sequence.
ACCESSION   DAOSFP010000064 DAOSFP010000000
VERSION     DAOSFP010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7424_length_8248_cov_4.010619"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..736)
                     /gene="ruvA"
                     /locus_tag="PLI16_03015"
     CDS             complement(134..736)
                     /gene="ruvA"
                     /locus_tag="PLI16_03015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOH83556.1"
                     /translation="MIHFIEGTLVEKNPVYAVIEAGGIGYFLNISLNTYTKLGEEGTK
                     CRLLTYMAIKNEAATPVGVTLYGFYTAEERDMYINLISVNGVGANTARLILSSLTTGE
                     IVSALANGQVATFEKVKGIGTKTAQKIIIDLKNKYSKLALEQDVVMGVNNTNKQEALS
                     GLMILGFNKNIAEKAIDKILRSDPSIDAVEELIKQALKIL"
     gene            complement(761..2215)
                     /locus_tag="PLI16_03020"
     CDS             complement(761..2215)
                     /locus_tag="PLI16_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOH83557.1"
                     /translation="MEKILLDLSNLDRAFPENFTQEQIAKAKTLFLKNLAETAHSYYE
                     GKIQTLPKAPVIGFNWFNVWYTPGVSKVSTTIRDQVDTSFHLTNRGNLVAVVSDSTRV
                     LGDGDCGPSGGLGVMEGKAFLMKYLGGCDGVALCIDSRNEKGEHDPDKIIQFVKMVQH
                     SFGAVNLEDISQPNCFKVLDVLREECDIPVWHDDAQGTACVTLAGLTNALKLVDKKIS
                     EIKIVLFGAGASNTTIARLIIADGGDPKKMVMFDTKGSLHAGRDDIKNDPRFYRKWEL
                     CAKTNPEKILSIEEAIKDADVLISLSTPGPNTIKKEWVSAMAPKSIVFACANPVPEIY
                     PYAAKEAGAYIVATGRGDFPNQVNNSIGFPGILKGALMVRAKKITDTMAIAAAHSLAD
                     YAEKRGIHPENIVPSMSEAAVFPQEAADVAMQAIRDGVARINISHQEAFARAEKDIRE
                     ARAVTQALVDEGFIQKPQQEMVQKAIDWAIQQVL"
     gene            2552..3037
                     /locus_tag="PLI16_03025"
     CDS             2552..3037
                     /locus_tag="PLI16_03025"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOH83558.1"
                     /translation="MPAKPKSIISVLLFAFLFCCFFLLPNFSTAQNSYGSDALCGEWW
                     TPERQGRIIFFKNGSTYSGKVAWMKKSIDPETGKPMLDKHNPNPSLRNRPITGMVLFY
                     DFKYNASKDKYVDGSLYDSDHGNTYSCWIKLIDKDVLELHGYIGFSLIGKSVYFTRVK
                     K"
     gene            3046..3729
                     /locus_tag="PLI16_03030"
     CDS             3046..3729
                     /locus_tag="PLI16_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOH83559.1"
                     /translation="MKTIVVLTGAGISAESGIKTFRDSDGLWEEHRIEDVATFDAWKK
                     NRSLVLDFYNQRRRQLATVQPNAAHRALARLEEKFHVQIITQNVDNLHERAGSSNILH
                     LHGELTKARSSLDESLIYDIGYNDIREGDKCSKGSQLRPHIVWFGEAVPNIMTAATLA
                     KHADILLVIGTSLVVYPAAGLLNYTPSLIPKYYVDPNAANTGHFNNLTVIKEKAGTGV
                     PALVKQLLS"
     gene            complement(3799..4380)
                     /gene="pth"
                     /locus_tag="PLI16_03035"
     CDS             complement(3799..4380)
                     /gene="pth"
                     /locus_tag="PLI16_03035"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOH83560.1"
                     /translation="MTQSESKKFLITGLGNVGDEYVNTRHNIGFVVLDALAQKLNVAF
                     ASGRHGQVAIARFKGKTLLLLKPSTFVNLSGKAVKYWLATEDIPRENALVVVDDLDLP
                     TGTLRMKSKGGDGGHNGLIDITTSLNSQEFARLRVGIGSDFARGFQVDYVLGKWTKNE
                     EKILLERIPVAVDAILSFICEGVNAAMNKYNNK"
     gene            complement(4444..5109)
                     /locus_tag="PLI16_03040"
     CDS             complement(4444..5109)
                     /locus_tag="PLI16_03040"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOH83561.1"
                     /translation="MRTVSMSGFLRGNVGKKDAKKIRRDGSVPCVVYGGKEQMHFFTD
                     TKNFQKIVFTPEVCFVKLTIEGKEMDVILKDIQYHPVTDNIMHADFMELKADKPIVMN
                     IPVKIFGTAPGVLKGGKVVQKYRKLKVKALPGNMPEVIDINIGSLEIGQNIKIADIPA
                     ENYSLLDNKNNTIVGVVVTRAVEEAATAAPTAEAGAPAAGAAAPAAGAAAPAADAKAK
                     AKK"
     gene            complement(5158..6111)
                     /locus_tag="PLI16_03045"
     CDS             complement(5158..6111)
                     /locus_tag="PLI16_03045"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007412001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOH83562.1"
                     /translation="MAQGISIFSGRATRYLSEKIALSYGKKLGDVSYACFSDGEFRPS
                     FDETIRGDDVFIIQSTFPPAENLLELFMMIDAAKRASARNIIAVIPYFGFARQDRKDQ
                     PRVPITSKLIANLLTAAGISRLITMDLHADQLQGYFDIPVDHLYASSIFVPYLQSLNL
                     NNLAMSSPDTGGTRRAGVYAKYLDADLVICFKQRPKPNQVGNMVVIGDVRDKNVILVD
                     DIIDTAGTITKAANLIKEQGAASVRAMCTHPVLSANACEKIEKSAMTELIVSDTIPLN
                     EECKKLSKIKVLSTANLFADVINSVVNCESISSHFKFTSLQ"
     gene            complement(6341..6853)
                     /locus_tag="PLI16_03050"
     CDS             complement(6341..6853)
                     /locus_tag="PLI16_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOH83563.1"
                     /translation="MLSKKMEKALNEQINAEFFSSYLYLSMAAYFERLNLTGFANWMR
                     VQEKEENAHGMIFFNYVNGRGGKVELLAIEKPASDWKNPIKVFEEVLKHEQKITALIN
                     NLVNIAIQEKDHATNNKLQWFIAEQVEEEANASALLEQLKLVEGNGQGLLLLDRELKT
                     RVFVDPTLQA"
     gene            complement(6937..7821)
                     /gene="atpG"
                     /locus_tag="PLI16_03055"
     CDS             complement(6937..7821)
                     /gene="atpG"
                     /locus_tag="PLI16_03055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOH83564.1"
                     /translation="MSGLKEVRIRIASVNNTKQITSAMKMVSAAKLRRAQGAILKLRP
                     YANKLSGLLSDLSVSTGLPEDIAYFKKRKVQKVLLVVINSNRGLCGAFNSNVIKAAVS
                     RINSEYNELNNTGNLHILSAGKTASDFFTKRKYNVIANYDSIYDNLTYEKSCELAEKL
                     MEYYANGTYDRIELVYNQFKNAVSQILTIEQFLPVEKLGQNGQSTLNQTDYIFEPEKS
                     IILKELVPKSLKIQTYKALLDSWASEHGARMSAMQKATDNAEELIKALKLSYNKARQA
                     AITKEIIEIVSGVNALKG"
     gene            complement(7853..>8248)
                     /locus_tag="PLI16_03060"
     CDS             complement(7853..>8248)
                     /locus_tag="PLI16_03060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012527.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOH83565.1"
                     /translation="QIKPMKKIAGTLKLDQAQYRELESFLKFGSDLDAATKAVLDKGA
                     RNVEILKQPQYTPMKVEHQIAIIFCGTKGLMQKVPVKSIIDFQEEFLHHLDLYHKELL
                     EKLGKGTLTDEMMAELEKAAKDIIPKYEA"
BASE COUNT         2129 a         1825 c         1735 g         2559 t
ORIGIN      
        1 gctgccctgt cgctgataaa tttataatca ggcgccatgc taacactgcc ggcaatggcg
       61 atcgacaata taaaggctgc agcaaaaatc tgaaatatgt atcgggccag tttcaatttc
      121 ggtaagtctt cggttataaa attttcaaag cttgttttat taattcttcc acagcatcta
      181 tcgacggatc tgaccgtaaa attttatcaa tggctttctc tgcaatattt ttgttgaaac
      241 ccaatatcat taatccagac aatgcttcct gtttgttagt gttattcacg cccataacga
      301 catcctgttc taacgctaat tttgaatatt tatttttcag gtcaatgatt attttttgtg
      361 ccgtttttgt gcctatacct tttacctttt caaaggtggc tacctgccca ttggcaaggg
      421 cggatacaat ctcgccggtt gtcagcgagg acagtatcag cctggcggta ttagccccga
      481 caccattgac tgaaatcaga ttgatataca tatcgcgttc ttcggcagta taaaacccat
      541 acagggtaac acctaccggt gtggctgctt cattttttat tgccatatac gtcaaaagcc
      601 ggcattttgt tccctcttca cccagctttg tataggtatt caaagagata ttcaagaaat
      661 acccaatacc cccagcctcg attactgcat acaccgggtt tttttcgaca agtgttcctt
      721 caataaaatg aatcatagat aaaataaaaa gggtacaaag ttacaaaact tgttgaattg
      781 cccaatcaat agctttttgt accatttctt gctgcggttt ttgtataaac ccctcatcaa
      841 caagcgcttg tgtaacagcc cttgcttcgc ggatgtcttt ttcggctctg gcaaaagcct
      901 cctggtgaga aatatttatg cgtgccactc catcgcgtat tgcctgcatg gccacatcgg
      961 cggcttcctg tggaaaaacg gccgcctcgc tcatggaagg tacaatattt tcgggatgaa
     1021 tgcctctttt ttcggcataa tccgccagtg agtgtgccgc agcaatagcc atagtgtcgg
     1081 taattttttt ggcacggacc atcagggcgc cttttaggat gcctggaaat ccaatggagt
     1141 tattcacctg attggggaaa tctcctcttc cggtagccac aatataggca ccggcttctt
     1201 tggcggcata ggggtatatt tcggggacag gattggcgca ggcaaacact atggattttg
     1261 gcgccatagc gctgacccat tcttttttta tggtgttggg gccgggagtt gacaaagaga
     1321 taagcacatc ggcatctttt attgcctctt ctatgctaag gattttttcg gggttggtct
     1381 tggcgcacag ctcccatttg cggtaaaagc ggggatcgtt ctttatgtcg tctcttccgg
     1441 catgcagcga gcctttagtg tcgaacatca ccattttttt cggatcgcct ccatcggcaa
     1501 taataagtcg tgcaatggtg gtattggaag cccctgctcc aaacaggact atttttattt
     1561 ccgaaatttt tttgtccaca agtttcaagg cgttggtaag gccggccagg gtaacgcaag
     1621 ccgtcccctg ggcatcgtcg tgccataccg ggatatcgca ttcttcgcgc agcacgtcca
     1681 gcaccttgaa acagttgggt tgcgagatat cttcgaggtt gacagcacca aaactatgct
     1741 gcaccatttt cacaaactga ataattttat cgggatcgtg ttctcctttt tcgttacggc
     1801 tgtcgataca caaggccact ccatcgcatc cgcccaggta tttcatcagg aatgccttgc
     1861 cctccatcac gcccagccct cccgaggggc cgcagtcacc gtcgcccagc acccgtgttg
     1921 aatcgcttac caccgccacc aggttgccac gattggtaag gtgaaacgag gtatccacct
     1981 ggtcgcgtat ggtagttgac acttttgaca cacccggcgt gtaccacaca ttaaaccagt
     2041 taaaacctat gacaggagcc tttggaaggg tttgaatttt tccttcataa tagctgtggg
     2101 ctgtttctgc aagatttttt aaaaacagtg tctttgcttt agcaatctgt tcctgggtaa
     2161 aattttcagg aaaagcccta tcaaggtttg ataaatccaa tagtattttt tccatagcct
     2221 taggtttaaa tttttccaat aatgtgtaaa agtaagtttt ttttcttaaa aacttcacaa
     2281 tattttataa gacaaaaaaa ccggttaaat caattttttg atgtatcaaa aataaattaa
     2341 gatttctcat ttcgcttcgc tctgttttgt agatattatg gttttcgcat ctgagaggag
     2401 gaaagcaggc gaagtataat gttaatttgc agaatgttat acgtattttt gtattaaaat
     2461 gatttttccc aacagttcaa aaaggttggt aaaaacacat tggctcagct gatttttcct
     2521 tatttttgta cactatatca actgtttcat tatgccagct aagcccaaaa gtatcatcag
     2581 cgtactgctt tttgcctttt tgttctgttg ttttttctta ttgccgaatt tctcaacagc
     2641 ccagaattcg tatggcagcg atgccctttg cggagaatgg tggactcccg aacgacaggg
     2701 aaggattatt ttttttaaaa acggcagcac atattcgggc aaggtagcct ggatgaaaaa
     2761 aagtattgac cccgaaacgg gaaagcccat gctggacaag cacaatccca atccgtcatt
     2821 acgtaaccgt cctattacag gcatggtact gttttacgat ttcaaataca acgcttccaa
     2881 agacaaatat gttgacggaa gcctgtacga ttccgaccac ggcaacacat attcctgctg
     2941 gataaaactt attgataagg acgtgcttga actgcatggt tatataggat tttcacttat
     3001 aggaaaatca gtatatttta cacgggtaaa aaaataaaag ccactatgaa gaccattgtt
     3061 gtattgacgg gggcgggcat cagtgcggaa agcggcatca aaaccttccg cgacagcgat
     3121 ggcttatggg aagagcaccg tattgaagat gtggctactt ttgatgcttg gaagaaaaac
     3181 cgttcgcttg ttttggattt ttacaaccag cgccgccggc agcttgcgac agtccaaccc
     3241 aatgcggcac atcgtgccct cgccagactt gaagaaaaat ttcatgtcca gattattacc
     3301 caaaacgttg acaaccttca tgaacgagcc ggttcaagca atattctgca cctgcacggc
     3361 gagcttacca aagcacgaag ttcacttgac gaatcgttga tttatgatat tggatataac
     3421 gacatacggg agggcgacaa atgctcaaaa ggctcgcaat tacgcccgca tattgtctgg
     3481 tttggcgaag cggtgccaaa tatcatgact gcagccacgc tggctaaaca tgccgacatt
     3541 ttgctggtga ttggcacctc gctggtcgtt taccctgctg cggggctgct gaattatact
     3601 ccttctctca tcccgaaata ttatgttgat cccaacgcgg ctaacacggg gcattttaac
     3661 aacctaactg tgatcaaaga aaaagccgga accggagtac cggctttggt gaaacagctc
     3721 ttatcctaac agaccttgtt gttgaatgca tggccgcaca acagggctta cctgccggca
     3781 cacagggcct ttaaatcctc acttattgtt atatttattc atggcagcgt ttacgccttc
     3841 gcagataaag ctgaggatag cgtcaaccgc tacaggtatg cgttccagta aaattttttc
     3901 ttcatttttg gtccattttc ccaacacata atcaacctga aatccccggg caaaatcgct
     3961 gcctattcca acacggaggc gcgcaaactc ctgactgttc agggaagtag taatatctat
     4021 caggccattg tgtccgccgt caccgccttt tgatttcatg cgcaaagtgc cggttggcaa
     4081 atcaagatca tcgaccacta ccagggcgtt ttctctggga atatcttcgg tggcgagcca
     4141 gtatttgaca gcctttccgc taaggttgac aaaagtcgat ggcttaagca gcagcaaagt
     4201 ttttcccttg aacctggcta tggccacctg tccatggcgc cccgaagcaa aggccacatt
     4261 taatttttgt gctaacgcat ccaacaccac aaaaccaatg ttatgccttg tattgacata
     4321 ttcatcgcct acattgccca agccggtaat caaaaacttt ttggattcgc tttgtgtcat
     4381 ctttttttaa taaaaaagct gccgaatact attttcggca gctttagtat tgtcggtcaa
     4441 aagttatttt tttgcttttg ccttggcatc agcggccggg gcggcagcac cggcagcggg
     4501 agcagcggcg cctgcagcag gtgcgccggc ttcagcagta ggagcagctg tggcagcttc
     4561 ttcgacagct ctggtaacca caacacccac gatggtgtta tttttattat caagcaacga
     4621 ataattctcg gcaggaatgt cagcaatctt tatgttctgg ccaatttcca gcgaaccaat
     4681 gttgatatca ataacttcgg gcatgttgcc aggcaaagct tttactttca gttttctgta
     4741 tttctgaacc acttttccac ctttaagaac gccaggggcc gtgccgaata tctttacagg
     4801 aatattcatg acaatgggct tgtcagcttt caattccatg aagtcggcat gcatgatatt
     4861 atccgtaaca ggatgatatt gaatatcctt caaaataaca tccatttctt tcccttcgat
     4921 ggtaagcttt acaaagcaca cttccggggt gaagacgatc ttctgaaaat tttttgtgtc
     4981 ggtgaaaaaa tgcatttgtt cttttccgcc ataaaccacg caaggtacgc taccgtccct
     5041 gcggattttt ttagcatctt ttttccctac gtttccgcga aggaaaccgc tcattgatac
     5101 tgttctcata atttttaatt gttaattgtt aattgtaagt gatatgttta tagattgtta
     5161 ttgcaaagag gtaaacttaa agtgtgaact gatggattcg caattgacca cgctgtttat
     5221 cacatcagca aacagattgg ccgtggacaa cacttttatc ttgctgagtt ttttacattc
     5281 ttcgttcagg ggtatggtat cggagacaat gagttctgtc attgccgatt tttcaatttt
     5341 ttcgcaggca ttggccgata ataccggatg tgtgcacata gcccgtacgc tggcggctcc
     5401 ctgttctttg atcaggttgg cggctttggt aatggtgccg gcggtgtcga taatatcatc
     5461 taccaggata acatttttgt ccctcacatc gccgatgacc accatattgc ctacctgatt
     5521 gggtttgggc cgctgtttaa aacaaattac cagatcagcg tcaaggtatt ttgcatacac
     5581 tccggcccgg cgggttccac cggtatcagg tgatgacatg gctaggttgt tcaggttaag
     5641 actttggaga taaggtacaa aaatgcttga tgcgtaaaga tgatccacag gaatatcaaa
     5701 atacccctgc aactgatcag catggaggtc catggttatg agccggctga ttccggcagc
     5761 ggtgagcagg ttagctatca gttttgaagt gatgggaacc ctgggttggt cttttctgtc
     5821 ctgacgtgca aatccgaaat atggaatgac ggcgataatg tttcgtgctg atgccctttt
     5881 ggccgcatca atcatcatga acaattcgag caggttttcg gcagggggaa aagtggattg
     5941 aataataaaa acatcatctc cgcggatggt ttcgtcgaaa gaaggacgaa attcgccatc
     6001 gctgaagcag gcataagaaa catcaccgag tttttttccg tagcttaagg ctatcttttc
     6061 tgaaagataa cgcgttgccc ttcctgaaaa tattgatatt ccctgtgcca taactgttta
     6121 ttttatgtct gcaaaattag gaattttttt actagtaaac cagccaaact aataatttta
     6181 ttattattca ttgattatta ttcagttatg ataatatttc caagaggaca atttccagat
     6241 atgtgtatgg tgtccggcgt tttttacagg cttactaaaa atacgacagg ctgccaaatt
     6301 ttgaaaaagg gcagcctgta gcaaaaaaat aatcctttta tcaggcctgt aatgtcgggt
     6361 caacaaaaac ccgtgttttc aactccctgt cgagaagcag cagtccctgc ccattacctt
     6421 ccaccaattt cagttgttcc agcaaggccg aagcatttgc ttcttcttca acctgttcgg
     6481 ctataaacca ctggagttta ttgttggttg cgtggtcttt ttcctgaatg gcaatattaa
     6541 caaggttatt gatcaaggcc gttattttct gttcatgttt taatacttct tcaaaaactt
     6601 ttatagggtt tttccagtca gaggccggtt tttcaatggc cagcaactca acctttccgc
     6661 cgcgtccgtt gacgtagtta aaaaatatca tgccgtgagc gttttcttct ttttcctgaa
     6721 cccgcatcca attggcaaaa ccggttaaat taagccgttc aaagtaagca gccatggaaa
     6781 ggtataagta tgaagaaaaa aattcagcgt tgatttgttc gttcagcgct ttttccattt
     6841 tttttgataa catgatgtcc tcctttttta ttatatattt taattgtaca aaattacata
     6901 atttctttta cataattggg tattaaaata tttattttaa cctttcaagg catttacacc
     6961 actgacaatt tcaataattt ctttggtaat ggcagcctgc cgggctttgt tgtaggagag
     7021 tttcagcgcc ttaatcagtt cttcggcatt atcggtggct ttttgcatgg ccgacatacg
     7081 ggcaccatgc tccgatgccc atgaatcgag gagcgcctta taagtttgaa ttttcaatga
     7141 tttaggcacc aattctttca ggataatact tttttccggt tcaaatatgt aatctgtttg
     7201 atttaaggtg ctttgcccgt tttgtccgag tttttcaacc ggcagaaatt gttcgatggt
     7261 cagtatctgc gatacggcat ttttgaactg attgtacacc agttctatcc tgtcgtaggt
     7321 gccattggca tagtattcca tcaatttttc ggccagttca cagctttttt cataggtaag
     7381 attatcgtaa atactgtcgt aattagctat tacattatat ttacgtttgg taaaaaaatc
     7441 gctggcggtt tttcctgccg acaaaatatg aagattgcca gtgttgttga gttcgttata
     7501 ttcactatta attcttgaca ccgcagcctt gataacatta ctgttaaaag cgccacagag
     7561 tccccggttg gagttgataa caaccaacaa tactttttgt acttttcttt ttttaaagta
     7621 ggcaatatct tcgggcagac ctgtacttac actaagatca gaaagcagtc cgctaagttt
     7681 gttggcatag ggccgcaact taaggatggc tccctgagcc ctcctcagtt tggcagccga
     7741 caccattttc atggcgctgg ttatctgctt ggtgttatta acagatgcta tccgtattct
     7801 tacttctttt aatcctgaca tgaatctttg ctattacagt cttcaaaaaa aatcaagcct
     7861 catatttggg aatgatatcc tttgcggctt tttccaattc ggccatcatt tcgtcggtaa
     7921 gggttccttt accaagtttt tcaagcagtt ctttgtgata gagatccaga tggtgtaaaa
     7981 attcctcctg aaaatctatt atacttttta caggaacttt ttgcatcaag cctttggttc
     8041 cgcagaaaat gatggctatc tgatgttcca ccttcatagg ggtgtactgt ggctgtttca
     8101 ggatttccac gtttctggcg cctttgtcca atacggcttt ggtggcagca tcgaggtcgg
     8161 aaccgaattt cagaaacgac tccaactcgc ggtattgtgc ctggtcgagt ttcagtgtcc
     8221 ctgcaatttt tttcataggt tttatctg
//
LOCUS       DAOSFP010000065         8162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7532_length_8162_cov_4.549278, whole genome shotgun sequence.
ACCESSION   DAOSFP010000065 DAOSFP010000000
VERSION     DAOSFP010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7532_length_8162_cov_4.549278"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            348..2963
                     /locus_tag="PLI16_03065"
     CDS             348..2963
                     /locus_tag="PLI16_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOH83566.1"
                     /translation="MIPLKQRLEDPKLKKRLKKTIVTLLVTLSVLGAAGVFVGFFYED
                     TVKRIIITELNKRLNTEIVVQDVENDIQFSIFRKFPYASVSFSNVKVMDAIKTQKPKG
                     ILLEASSLSLQFSILDILFGRYKIKKIEAEEGNVSIKIYKNRSDNYHFWKPSADTSAS
                     DFALDLQKIALDDMGLSYVDYGRKQDYKVFARDVILKGKFSDTEYTLNIDGRVFVYLI
                     KLEQDIYLANQPADLDVILYVNSGNDYVTFKEGGIDIGKLVFDITGTINYAESNTLLD
                     LRVEGKKMKIQSLINQLPSRYRELLGDYEFKGDFNFIALVKGYTGEKTNPLVSVAFGL
                     KDGDIIQKQNSITLSHVCFDAEFTNGSGHQKATTTLSVRGFKSQLNDGNIAGSIQIKN
                     FIKPEIALKLAASLDLKDIFLFVKTDTVQSASGKILLNTDIKGVIERPDGFTVRDFIA
                     STSTGTLEVQDAGIELKGGRHNFSGLNGLFTFSNNDIETGQFSGKYMATDFTMRGKFK
                     NIIPYLFIENQSLLIDAGFHSDFIDLGEILEYSSGKKDTVYRMEMPENVDFSLGIDVG
                     KLTFKKFTATNITGKVTMKDKQFVAHDISLKAMNGNIIFSGLVDGAAPDKLLISCDAS
                     IKKVDIRRLFGELENFGQTSLQERNLKGQLSATLQFASVWSSTFKVEKPTIYAKADIV
                     IENGALIDYEPLSGLSKYLKGRDLKNVSFETMKNTIEIKNEVIQIPTMEINSNAIDFQ
                     VNGTHGFDQKIDYHLSVLLSDLGTVKEKNMQAEDIGVITDDGLHKERYFFRITGTVDE
                     PVYHTIDKEGYKANIKTNLAKEKESLKEILNREFGWFKKDTVITKEKGQKPKEKYEFN
                     VVWDEDEDSNKKEVE"
     gene            complement(3013..3408)
                     /locus_tag="PLI16_03070"
     CDS             complement(3013..3408)
                     /locus_tag="PLI16_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOH83567.1"
                     /translation="MFNFFDKVFFLKFIKFGVVGGSGVLVDFGTTYVCKEWLKIPKYI
                     SNAMGFTVAATWNYIFNRIWTFESKNPEIAWEFFRFFSVSLVGLGINTLILWLLVSKF
                     KMNFYLSKLFAIGVVMIWNFALNLLVTFR"
     gene            complement(3497..5095)
                     /locus_tag="PLI16_03075"
     CDS             complement(3497..5095)
                     /locus_tag="PLI16_03075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH83568.1"
                     /translation="MNKRFAIILPLFFALTLIAGILAGKYLLPGAGNATSLFSGSHYN
                     KLNDVMNYIENDYVDTINKSKLLENAISGLLETLDPHSVYIPAEEFNEANAPLAGNFD
                     GIGVQFRLIRDTIVVINTVAGGPSEKEGIHAGDRIVKIDGKNVAGKKITNDEVMKKLK
                     GPRGTKVKVGIFRRDEHKMMDFMITRNVIPTYSVDVAYMVNGNIGYIKLNTFSATTTD
                     EVYDAISSLLSQGMKKLIFDVRDNGGGYLDAAINIADQFLPDKNMIVYTEGSHRARKN
                     NYSKNGGLFENGSLVILINEFSASASEILAGAVQDNDRGTIIGRRSFGKGLVQEQIKL
                     RDGSAIRLTVARYYTPTGRCIQKPYSSDIYEYYMDFYKSLLDEDSEASDSLNFNDTVR
                     YKTPKGKIVYGGGGIMPDIYIPNKSEFVSAYYRKILSKSSLLSDFAFDYADQNRKKLR
                     QYQNAKNFVALFQISDDLFNDFVAYAASKGVARDDEGIKPAAPRLKVLLKANIGRNIF
                     DDPAFYPVYLQDDITFKKALEVLK"
     gene            5364..5882
                     /locus_tag="PLI16_03080"
     CDS             5364..5882
                     /locus_tag="PLI16_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOH83569.1"
                     /translation="MFQLLFDKEIYDKFILEKISSAKKFIWIGTADIKDMYIARRTDM
                     VPFLEILAALIRKKVEVRLIHAKEPGPAFRKDFDKYPELFSGLERVLCPRVHFKMIII
                     DGVVAYCGSANLTGAGMGAKSEHNRNFENGFMTDDVKLIGPLMEQFDSVWRGAHCKKC
                     GRKEFCLDCPLE"
     gene            complement(6010..6276)
                     /locus_tag="PLI16_03085"
     CDS             complement(6010..6276)
                     /locus_tag="PLI16_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOH83570.1"
                     /translation="MQGFLYILFSNKINKYYVGSTNDLERRLYEHNIGHSVFTKTGIP
                     WTLVFSKPFDTLENARQEERRIKKCKSRRYIENYVNSAVEHPDT"
     gene            complement(6280..6352)
                     /locus_tag="PLI16_03090"
     tRNA            complement(6280..6352)
                     /locus_tag="PLI16_03090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6317..6319),aa:Thr,seq:cgt)
     gene            complement(6490..7101)
                     /locus_tag="PLI16_03095"
     CDS             complement(6490..7101)
                     /locus_tag="PLI16_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83571.1"
                     /translation="MASRVTSFFKKIIPENKIARFIIVFLLIFLPWHFIDDYMQMAHY
                     GPIFVNAWFFAYHLLLKSLMFLSIHLTSLFTDVAITTSYRVIYLGGYGSLSIGRPCLC
                     ADVMMLLVAFVVAYPGPWKKKLWFLPVGLASIHLMNVLRISALCLTKIYRPEWMYFNH
                     NYVFNFIILGFTFMLWVIWIAKISPDNNNKLKAGNKKENRNAD"
     gene            complement(7108..8091)
                     /locus_tag="PLI16_03100"
     CDS             complement(7108..8091)
                     /locus_tag="PLI16_03100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH83572.1"
                     /translation="MSIEKLKKDDYIHGGTQKDFYDENQREACRCPLCDADDYTILGT
                     ERGLSVVKCNSCKLIYTNPRANNAQENYFGDADTFYNEARLIFKGKKVHHRDRNYEYE
                     VKKIKKYKNGGKLLDVGTNMGFFLRKAREAGFETEGVEPSPALAKIATENWGLKIHNS
                     FLEQATLPENNYDVITLIDVFEHVTNPKELLKKNYSLLKSDGIMAIKVPNGDYNHFKM
                     KLGQLTGKDKKMDIWDCYEHVVHYTPATFSKMAESCGYKIKKCFIPLPIHSPVWANLV
                     GHYYQYPSPVILDWKRILLRNIFYHLGKIELVLRGKTRFGLDLMFIIEKKQ"
BASE COUNT         2425 a         1570 c         1769 g         2398 t
ORIGIN      
        1 cagcaacagc atcattacgg cacttaaaac atattttttc atagaatttt ttacaaaatt
       61 atattaaata aataaaacca tagaaatccc tgcccgggat ttattaacaa atttacaaaa
      121 aatagacatt agcagacttt ttttttgttt ttgtggcgaa aaatgtgaaa aatgtggttt
      181 ttcactatga aaaatcatgg catcaatcac cctattgagc tcattgatat ttcaatttga
      241 tattattgat aatctgtcgg aggatataaa acttctattg tgaataataa accccaaaaa
      301 aatcgttttg ttatataagc aagtattaat tttgtaaaaa gattattgtg atccccctga
      361 aacagcggct tgaagacccg aaattaaaaa aacgtttaaa gaaaacaata gtgacgctat
      421 tggtcacgct atccgtgttg ggcgctgcag gggtttttgt ggggtttttt tatgaagata
      481 cggtaaaacg tattatcatc acagaattaa acaagcgact taataccgag attgtggtgc
      541 aggatgttga aaacgatatt caattttcga ttttcaggaa gtttccctat gcgtcggtaa
      601 gttttagtaa tgtgaaagtg atggatgcca taaaaacgca gaagcctaag ggtattctgc
      661 tggaggcatc gtcgctgagc ctgcagttca gcatactgga tatacttttt gggaggtata
      721 aaataaaaaa aattgaggcc gaagagggaa atgttagcat aaagatatat aaaaaccgca
      781 gtgacaatta tcatttctgg aagccatccg ctgacacttc ggcaagtgat tttgccctcg
      841 accttcaaaa aatcgcactg gacgatatgg gtcttagtta cgtggattat ggccgaaagc
      901 aagattataa agtatttgcc agagatgtta tcctgaaggg caaattttca gatacggaat
      961 ataccttgaa tattgacggc cgcgtgtttg tgtatcttat aaaactggag caggatatct
     1021 acctggcaaa tcagccggcc gaccttgatg taatccttta tgtgaattcc ggcaacgatt
     1081 atgtaacatt caaagaagga ggcattgata tcggcaaact ggtgtttgat atcacgggca
     1141 ctatcaatta cgccgaaagc aacacccttt tggatttgcg cgtggaaggt aaaaagatga
     1201 aaattcaatc gctcattaac cagttgccgt cacggtaccg tgagttgttg ggcgattatg
     1261 aatttaaggg cgattttaac ttcatcgcat tggtaaaagg atataccggg gaaaaaacca
     1321 atccactggt gtccgttgcc tttggcttaa aagacggaga catcatacaa aaacaaaatt
     1381 ccattacgct aagccatgtt tgtttcgatg cggaattcac caacggatcc ggccaccaga
     1441 aagccacgac aaccctgtcc gtcaggggct ttaaatcaca attaaatgat ggcaacattg
     1501 cgggcagcat ccagataaaa aattttatca agccagagat agccctgaaa cttgcagcca
     1561 gccttgatct taaagatatt ttcctttttg tgaaaactga tacggtgcag tcggcttcgg
     1621 gaaaaatcct tttgaataca gatatcaaag gtgttatcga acgccccgac ggtttcacgg
     1681 taagggattt tatagccagt acttctacag gaactctcga agtacaggat gccggcattg
     1741 aacttaaagg cggaaggcac aatttttcgg ggcttaacgg cctgtttacc ttcagcaata
     1801 atgatattga aaccgggcaa ttcagtggca aatatatggc tacggatttc acgatgaggg
     1861 gcaagttcaa aaatatcatt ccttatcttt ttatcgaaaa tcaaagcctg ttaattgatg
     1921 ccggttttca ttccgatttc attgatttgg gcgaaattct tgaatattct tcggggaaaa
     1981 aagacacggt ttatcgaatg gagatgcccg aaaatgttga tttcagcctg gggattgacg
     2041 ttgggaaatt gacctttaaa aagtttactg ccaccaacat cacgggcaaa gtaactatga
     2101 aagacaagca gtttgtggca catgatattt cgttgaaggc gatgaacggg aacataattt
     2161 tttcaggtct ggtggatggt gccgctccgg ataagcttct tatcagttgc gatgcttcta
     2221 taaaaaaagt ggacattcgg agacttttcg gagagttgga gaatttcggg cagaccagcc
     2281 tgcaggaacg aaaccttaaa ggccagcttt ctgccacatt gcagtttgct tcggtgtggt
     2341 cttcgacatt taaggtggaa aaacccacta tttatgccaa ggctgatatt gttattgaaa
     2401 acggagccct cattgattat gaacctctca gcggtttgtc gaaatacctc aaaggccgcg
     2461 acctgaaaaa tgtttcattt gaaactatga aaaacaccat agaaattaaa aatgaggtca
     2521 tccagattcc cacaatggag atcaacagca acgccattga ttttcaggtg aacggaacac
     2581 acggttttga tcagaagata gattatcatc tgagcgtgtt gttgtcggat ctgggtacgg
     2641 tgaaggagaa aaacatgcag gctgaagata tcggggttat taccgatgac ggattgcata
     2701 aagaaaggta ttttttccgc ataaccggca ctgtcgatga gcccgtgtat cataccattg
     2761 acaaggaagg gtacaaggcc aacataaaga ccaacctggc aaaagaaaaa gaatcactga
     2821 aagaaatact caaccgcgaa ttcgggtggt ttaaaaaaga cacggttatt accaaagaaa
     2881 aaggtcagaa accaaaggaa aaatacgagt tcaatgtggt gtgggacgaa gatgaggata
     2941 gtaataaaaa ggaagtggaa taacatcagg cagggcattg ccctttcgga gttttttttc
     3001 agattgtttt ttttagcgga aggtaaccaa caaattcagg gcaaagttcc atatcattac
     3061 cacaccgatg gcaaagagtt ttgacaggta aaaattcatt ttaaatttgc ttaccagcag
     3121 ccaaagtatg agtgtgttga tgcctaagcc aaccagggaa acactgaaaa accggaaaaa
     3181 ttcccaggct atttcggggt ttttgctttc aaaggtccat atcctgttaa agatataatt
     3241 ccaggtagca gccactgtaa aacccatggc gttggagata tatttcggta ttttcaacca
     3301 ttctttgcac acataagtag tgccgaaatc caccaaaacc ccggagccgc ccaccacacc
     3361 gaatttgatg aatttcagga aaaaaacttt atcgaaaaaa ttaaacataa cgtgaaagtc
     3421 ttcgtctgca aaaataggtt gtttttacgg tttgtgaaac aaaaactttt tcgtgttctg
     3481 ctccaaaaag aaatttttat ttgagtactt ccagggcttt tttaaaggta atgtcatctt
     3541 gtaaatatac cggataaaat gccggatcat caaaaatatt acggcctata ttggccttaa
     3601 gcaggacttt cagccggggt gccgcaggct ttattccctc gtcgtcacgc gcaacacctt
     3661 ttgatgctgc ataagcaaca aagtcgttaa aaaggtcatc agatatctgg aagagggcaa
     3721 caaagttttt ggcattttga tattgtctca gttttttgcg gttttggtcg gcatagtcga
     3781 aggcaaaatc gctgagcagg ctgctcttac tgagaatttt tcgataatat gccgaaacaa
     3841 attcggattt gttggggata taaatgtcgg gcatgatgcc accgccgcca tacactattt
     3901 ttccttttgg tgttttatac ctcacggtat cattaaagtt caggctgtcg cttgcctcgg
     3961 agtcttcatc gagcaggctt ttatagaaat ccatataata ttcgtaaata tcggaggaat
     4021 aaggcttttg tatgcagcgg ccggtagggg tataatagcg tgcaacagtc aggcgtatgg
     4081 ctgatccgtc gcggagtttg atttgttcct gaaccagtcc tttgccaaaa gaccgcctgc
     4141 cgatgatggt tcctctgtcg ttgtcctgaa cggcgccggc caatatttcg ctggctgagg
     4201 ctgaaaattc gttgataagt atcaccagac tgccgttttc gaagaggcca ccgtttttcg
     4261 agtaattatt tttgcgtgcc cggtgtgatc cttcggtgta cactatcata ttcttgtcgg
     4321 gcaaaaactg gtcggcaatg ttgatggccg catcgaggta tcctccaccg ttgtcgcgaa
     4381 catcaaaaat caactttttc attccctgag aaagtaagga tgagatggca tcatagactt
     4441 cgtcagttgt tgttgccgaa aatgtattca atttaatata accaatattt ccgtttacca
     4501 tataagctac atctacggaa tacgtgggaa tgacattccg ggtaatcata aaatccatca
     4561 ttttgtgttc atctctgcgg aagatgccca ccttaacctt ggtgccgcgc gggcctttta
     4621 gttttttcat tacctcatcg ttggtaattt tttttccggc tacatttttt ccgtcaattt
     4681 tgacgatacg gtcaccggca tggattcctt ctttttctga tgggccgcca gctactgtgt
     4741 ttatcaccac aatcgtgtcg cggatcaggc gaaactgcac gcctatgccg tcgaaattgc
     4801 ctgccagcgg agcgttggcc tcattaaatt cttcggcagg gatataaaca gaatgagggt
     4861 cgagtgtttc gagcaatccc gagatggcat tttcaagcag cttgcttttg ttgatggtat
     4921 ctacataatc gttttcgatg taattcatca catcattgag tttattgtaa tggctcccgc
     4981 tgaaaagcga tgtggcattg ccggcgccgg ggagtagata tttcccggcc agaatacccg
     5041 cgatgagtgt gagtgcaaag aataatggga ggattattgc aaaacgttta ttcatttaag
     5101 aattgttctt tagaattaaa agatgcggtg gattgtgaat gcaaaattat gctttttggt
     5161 gttaatagtt ccggattaat tcacgatcca atcatttttt tgaagtgccg gcagagatta
     5221 ttttattgta aataaacaac tttcgcaatt caaacaaaaa aaccgtttca aaacttatac
     5281 ttccacccgc tacgccccag ttcactgcct ttctattgcg taatttgtga ttaatactat
     5341 atttgcatga aatataaggc gctatgtttc agttgctttt cgacaaggag atttacgata
     5401 aatttattct ggaaaaaatt tcttccgcaa aaaaatttat ctggatcggc accgccgaca
     5461 tcaaggacat gtacatagcc cgccgaacgg atatggtgcc ctttcttgag atattggctg
     5521 cattgataag aaaaaaagtg gaggtgcgtc ttatccatgc caaagagccg ggaccggcat
     5581 ttcgtaaaga ctttgacaaa taccccgaat tgttttcagg actggagagg gtcttgtgtc
     5641 cgcgtgtaca tttcaaaatg attatcatcg atggtgtggt tgcctattgt ggtagcgcca
     5701 atcttaccgg tgccggtatg ggtgcaaaaa gcgaacataa ccgaaatttt gaaaacggat
     5761 ttatgaccga tgatgtaaaa ctgataggtc ctttaatgga gcagtttgat agtgtgtggc
     5821 gcggagcaca ttgcaaaaaa tgcggcagga aagaattttg cctggattgt cctttggagt
     5881 gaattctttt atagtgtgcg gagagaaagc ctcaactatt tacttgttaa ttttattact
     5941 gaaaagaatg tacagaaacc cttgcatcag agccactaat gggactcgaa cccacgacct
     6001 tcccgacact taggtgtcgg gatgctcaac cgcggagttc acataatttt cgatgtatct
     6061 tctgctttta cactttttaa ttcgtcgttc ttcttgtctg gcgttttcaa gagtgtcaaa
     6121 gggtttagaa aaaaccagtg tccatggtat tcccgttttg gtaaatacag aatgcccaat
     6181 gttatgctca tacaaacgtc tttccaggtc gtttgtagac cccacataat acttgttaat
     6241 tttattactg aaaagaatgt acagaaaccc ttgcatcaga gccactaatg ggactcgaac
     6301 ccacgaccta cgcattacga atgcgttgct ctaccaactg agctaaagtg gcaagaaaaa
     6361 ataacagaac gttgatgcaa aagtagaaaa aattttttaa aaaaactgtt acttgagcaa
     6421 atatcagtca tattttgttc cgggggtatc aaatggatca ttttccttgc cattcaatgt
     6481 gagcctggtt tagtccgcgt ttcggttttc ttttttattg ccggctttga gtttattgtt
     6541 attatcgggc gagattttag ctatccagat tacccacagc ataaaggtaa atcccaaaat
     6601 gataaagttg aagacataat tatgattaaa atacatccat tcggggcggt agattttagt
     6661 aaggcataag gcagaaatcc ttaacacgtt catcaaatga atagaagcca atcccacagg
     6721 caggaaccat agttttttct tccagggccc gggataagca acaacaaagg cgacgagcaa
     6781 catcataaca tcggcacaaa ggcatggccg tccgattgac agagagccgt agccgcccag
     6841 ataaatgaca cgataagatg tggtgatagc aacatcggtg aacaatgaag taagatggat
     6901 gctcaaaaac atcagacttt ttaacaacaa gtggtatgca aaaaaccatg cgttaacaaa
     6961 gataggcccg taatgagcca tttgcatata atcgtcaata aaatgccagg gaaggaaaat
     7021 gagaagaaag acaatgataa accgggcaat cttgttttct ggtattattt ttttaaaaaa
     7081 acttgtaacc cttgatgcca ttggtagtta ttgttttttt tcaatgataa acatcagatc
     7141 gagtccgaaa cgggttttgc ccctgagcac taactcaatt ttaccgaggt gatagaaaat
     7201 gtttctcaat aaaattcttt tccagtccag aataacgggt gaggggtatt gataataatg
     7261 tcccactaag ttagcccata cgggagagtg aataggcagc ggaataaaac atttttttat
     7321 tttgtatccg caactttctg ccattttgct gaaagtggcc ggtgtatagt gtaccacgtg
     7381 ttcgtagcaa tcccagatat ccattttttt gtcctttcct gtgagctggc ctaacttcat
     7441 tttaaaatgg ttgtaatcgc cgttgggcac tttgatggcc attattccat cagatttcag
     7501 cagggagtaa ttttttttta gcagttcttt ggggttggta acgtgttcaa aaacatcgat
     7561 cagtgtaatg acatcataat tgttttcggg cagtgtagct tgttccagaa aagaattatg
     7621 aattttcagc ccccagtttt cggtggcgat tttagctaag gcaggactgg gttcaacgcc
     7681 ctcggtttca aaaccggcct ccctggcttt tctcaggaaa aatcccatgt tggtgccaac
     7741 gtcaaggagt tttccaccat ttttatattt tttgattttt ttaacttcat actcataatt
     7801 gcggtcgcgg tgatgaactt tttttccttt aaaaatcaag cgggcttcat tataaaaagt
     7861 atcggcatca ccgaaatagt tttcctgtgc attattggcg cgggggttgg tatatattaa
     7921 tttgcagctg ttgcatttaa ccacagaaag tcctctttcg gtgcccaata tagtgtaatc
     7981 atcggcgtcg cacaagggac accggcaagc ctcacgttgg ttttcatcat agaaatcttt
     8041 ttgagttcct ccatgaatgt aatcgtcttt tttaagtttt tcgatactca ttttttggga
     8101 ttttaagatg cacagccaat ttcaggtaac aacatgagca aacagaaaac gatctgcttg
     8161 tc
//
LOCUS       DAOSFP010000066         8024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7717_length_8024_cov_4.350734, whole genome shotgun sequence.
ACCESSION   DAOSFP010000066 DAOSFP010000000
VERSION     DAOSFP010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8024
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7717_length_8024_cov_4.350734"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLI16_03105"
     CDS             complement(<1..343)
                     /locus_tag="PLI16_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83573.1"
                     /translation="MSITVISGLFLLISVIIIGLGLLMLILGIIQKKTGQWVSGILIT
                     LFALVFCIIGLVWLVGKKINTACDNSKQSEYYDQPYDDDMEENYGDTVYHGDESMATD
                     ENYISGFIQDDD"
     gene            complement(349..1551)
                     /locus_tag="PLI16_03110"
     CDS             complement(349..1551)
                     /locus_tag="PLI16_03110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH83574.1"
                     /translation="MNYLSDRINNLSESETLAMTQKSRELSAQGFDVINLSIGEPDFN
                     TPEFIKTAAKEAIDKNFTHYPPVPGYPELRQAICKKLKRDNDLDYAPEQIVVSTGAKH
                     SIANVMLALVNPGDEVIVPAPYWVSYKEIVKLADGKSVIIPGGIDTNFKVTPEQIEAA
                     ITEKTKVFIFSSPCNPTGSVYSKEELFAMAKVFERHKNIFIISDEIYEYINFVGKHES
                     IAQFDFIKDQVIVINGVSKGYAMTGWRIGYIAAPLPIAKACNKLQGQVTSGACSIAQK
                     ASFTAIDTDPKLTPEFKTMLDAFRERRDLLLGLLKEIPGIITNTPDGAFYVFMDVKYY
                     FGKTDGTATINGSNDLSMYLLNTAHVAMVPGTAFGDPNCLRFSYATSKEKITEAVKRI
                     KETLAKFK"
     gene            1885..3096
                     /locus_tag="PLI16_03115"
     CDS             1885..3096
                     /locus_tag="PLI16_03115"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOH83575.1"
                     /translation="MVSVENWKAKLSDSLKFYFQEDYKLMQRLIVLLAFAGFIGHFLF
                     YFVLSDIMGYWESLVLRIIVALLFVSLLLLPRHRKLSTFGMWYYEMVYVLVFPVFFAI
                     NLIENDINVYWSISVMFSAILYGLLTHPPKAVVLYPLSVFITLLVMNYIRGGLPQLGE
                     AIIMHFPAYFMVILLGLIQTTIRRAYAAAERERQHSEKLLENILPRKIINILKTNPDT
                     IAEKYDHCSILFVDVVDFTPFAEKSAPEEVVNILNEIFTLFDRLTGKYKLEKIKTIGD
                     AYMVVSGVPEPRPDHSEAIADMALDMLRVVDEYNIRSGNHFRVRLGINSGPAIAGVIG
                     KMKFAYDIWGDTVNTASRMESLGVPGRIQTTEVTYEMLKNKFNFEYRDVLDVKGKGKL
                     KTYFLESRKMA"
     gene            3237..3758
                     /locus_tag="PLI16_03120"
     CDS             3237..3758
                     /locus_tag="PLI16_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83576.1"
                     /translation="MEDFLDILKYTIPSGVVFATAFFILRAFLQNGTQKKLLELKLSN
                     QNVITPVRLQAYERVILLLERISPSSLVLRVARPGMSAVELQAALLQTVRDEFEHNLS
                     QQIYMSGTAWDMTRNAKEEMIKIINLASTRLNDEATAADLGSVILELAMSQDKLPVNM
                     AIDYIKKEARQLY"
     gene            complement(3782..4855)
                     /locus_tag="PLI16_03125"
     CDS             complement(3782..4855)
                     /locus_tag="PLI16_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOH83577.1"
                     /translation="MKKHILSCILSLVCLTIYAQIEKIEKTIELKTTPVKNQAMSNTC
                     WSFATLSFFESELLRMNKGEYDLSEMYYVRMAYPIKANNYYHFQGHSNFGPGGQAHDI
                     TQLFKKFGAMPETVYSGLNGKKNHEQLLMDEALEHIMDSVVKKAEGSADKKWELMLGS
                     IMDIHLGPIPSEFSYNGNTYSPESFAASLGLNADDYVELTSFSHHPYYESFVLEIPDN
                     WMFEKYYNLPLEDLMAVMENSLNKGYTLVWDGDVSDKKSFANNGGVAETGNEKEKVSQ
                     ETRQQAFEDFTVTDDHLMHITGLAASKDGAKYFLVKNSWGEKKGNEGYWYLSENYVRL
                     KTIAIMVHKDVLPAPIKTRLNID"
     gene            4928..5341
                     /gene="arfB"
                     /locus_tag="PLI16_03130"
     CDS             4928..5341
                     /gene="arfB"
                     /locus_tag="PLI16_03130"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOH83578.1"
                     /translation="MMDIEALLKEFSFRTSRSSGKGGQHVNKVSTKVEVIFNVANSML
                     LSNDEKEKILAGAGKHGRDDGTVFMICSEGRSQYLNKKKAIEKIIRLLEKCLAPVKSR
                     VPTAVPDEEKEKRMAEKKKTAEKKRQRKNNPEDQF"
     gene            complement(5375..6151)
                     /locus_tag="PLI16_03135"
     CDS             complement(5375..6151)
                     /locus_tag="PLI16_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH83579.1"
                     /translation="MVKSKHKYKTGLVLSGGGLRGFAHAGIIKALNEAGIFPDVVSGT
                     SAGSIVGAFYADGWKPDEIFKVFANKSTYKFLEFIVPNKGLLRMTGLFKIMLQHLKAR
                     TFEELQIPLFMAVTNLDDACVEYISKGDLAKYVIASCTVPIVFQPTAIDGVTYVDGGV
                     LDNLPIAPIENDCEFLIGANVNPVLPENKYNGMTNVADRVVHMAINKVNESKIGKFDI
                     FVEPAELHKYSMMDTSKGPKMFEIGYKETIRILKEKGFLN"
     gene            complement(6284..6646)
                     /locus_tag="PLI16_03140"
     CDS             complement(6284..6646)
                     /locus_tag="PLI16_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83580.1"
                     /translation="MSTHLNIKLLTNEAIINSWDVYYTFDDGQSFFGLITITNKRLLF
                     DTKIIGAAEIMLETSPLLKNHISDYVILSKKHISSVEILKDSSGNKVVMTMDNGEKHT
                     LDRKMLAVDQIAEALNFN"
     gene            6838..>8024
                     /locus_tag="PLI16_03145"
     CDS             6838..>8024
                     /locus_tag="PLI16_03145"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOH83581.1"
                     /translation="MKKVLILGAGMVANPIIQHLLSKNIRLTVAALNYDEAKRMVGQA
                     ENGKAVHWTVDDQAGLNTMVAENDVVVSLIPYAFHVMVAKCCLKHKKHLVTASYAKPE
                     MYALDAAAKEAGVIFLNEVGLDPGIDHMSAMRIIDHVHGKGGKIEAFYSLCGALPAPE
                     SCDNPFNYKFSWSPKGVVLAGNNDAVYLMKGKKVEIPTADLFKNPMIVDFPEVGPMDV
                     YPNRDSLSYVDIYGIPETKTMFRGTFRFKGWCETLDVMKALKCTLPEKHDFSGKTFAG
                     AVALVNGLSDEKNIKKQVAALLHIDENAYALNAMEWLGLFSNTPMNRTEDSMYEITSD
                     LMIEKMMITENDRDMVVMMHVFKAVYPDGTAEIIKSRMLDYGTLKTDTSIARTVALPA
                     ATAV"
BASE COUNT         2220 a         1577 c         1797 g         2430 t
ORIGIN      
        1 tgtcgtcgtc ttgtataaat ccggagatat agttttcatc tgtagccatt gattcatcgc
       61 catgatatac agtatcgccg tagttttctt ccatatcgtc atcatacggc tgatcataat
      121 actccgactg tttagaattg tcacaagccg tgttaatttt ttttcctacc agccatacca
      181 ggcctatgat acaaaatacc agtgcaaaca gcgtgatcag gatgccggaa acccattggc
      241 cggttttttt ttggatgatg ccaagtatga gcattaacag ccccagtccg ataatgatga
      301 cggaaattaa taaaaataat ccgctgataa ccgttattga catagagttt atttaaattt
      361 agctaaagtt tctttgatgc gttttacggc ttcagttatc ttttctttgg atgttgcata
      421 cgaaaacctc aggcagttgg ggtctccgaa agctgttccc ggaaccatgg ccacatgtgc
      481 cgtgttgagg aggtacatgc tcaaatcgtt actgccgttg atggtagcgg ttccgtcggt
      541 ttttccgaaa taatatttta catccatgaa aacataaaaa gcgccgtcgg gggtgttggt
      601 gatgataccg ggaatttctt tcagcaggcc gagcagcagg tcgcggcgtt cgcgaaaagc
      661 gtccagcatg gttttaaatt ccggagtgag tttgggatca gtgtcgatgg ccgtaaagga
      721 tgctttttgg gcgatggagc aggctcccga agtaacctgt ccctgcagct tattacaggc
      781 cttggcaata ggcagcggtg ctgcgatata gccgatacgc cagcccgtca tggcataacc
      841 tttcgaaaca ccattgataa caataacctg atctttaata aaatcaaact gggcgatgct
      901 ttcgtgttta ccgacaaaat tgatgtattc ataaatttcg tccgaaatga taaagatgtt
      961 tttatggcgt tcgaaaacct ttgccatggc aaaaagttct tctttgctat acaccgagcc
     1021 tgtaggattg caaggactgc tgaaaataaa cactttggtt ttttcggtga tggcggcttc
     1081 aatttgttcc ggagtaactt tgaaattggt atcgatgcct cccggaataa taacagactt
     1141 gccatcagca agttttacaa tctctttgta ggaaacccag tagggcgcag gcacaatcac
     1201 ttcgtcgccg gggttgacca gtgccagcat cacgttggcg atagagtgtt ttgctccggt
     1261 agaaaccaca atttgttcgg gggcataatc aaggtcatta tcgcgtttca gttttttaca
     1321 aatggcctgg cgcagttcgg gatatcccgg caccggtggg taatgtgtaa agtttttgtc
     1381 aatagcctct ttggcagccg ttttaataaa ttcaggtgta ttaaaatccg gttcaccgat
     1441 gctgaggttg ataacgtcga agccctgcgc acttaattcg cggcttttct gagtcatagc
     1501 caatgtttcc gactctgata aattgttgat tctgtccgat aaatagttca tagttcaatt
     1561 ttttttcaaa aataacaatt aatattatgt tggcagctat tgaaaagaga tttttaggtt
     1621 aattttgaaa tggtaccggt tccgggttaa cctggcgttt cattatgttg aaacgatcat
     1681 tatttattca tatcgtgagt aatattttta atttttacgg agttttaaaa ttaactattt
     1741 ttgaacaggg aaactgtggt gtcgtgtcaa gtgatatacg cgcaatgcca aacaaataat
     1801 caaatataat tttatctgta tgtaaggctt gatgcgtagg ggaagtattt tagggataat
     1861 aattttttaa gaaatcatca ttttatggta tctgtagaaa actggaaagc aaaattatcg
     1921 gacagcctga aattctattt tcaggaggat tataaactga tgcaacggct gattgtcttg
     1981 cttgcttttg ctggttttat tgggcatttt cttttctatt ttgtattatc agacatcatg
     2041 ggttattggg aatctcttgt cttacggatt atcgtcgcct tactctttgt atccctgttg
     2101 ctgttaccca ggcacagaaa gctgagcacc tttggtatgt ggtattacga aatggtttat
     2161 gtgctggttt ttcctgtttt ttttgcaatc aaccttattg aaaatgatat taatgtctat
     2221 tggtctattt ctgtgatgtt ttcggctatt ttgtatggat tgcttacaca ccctcctaaa
     2281 gcagtggtgc tatatccgtt gagcgttttt atcacactcc tggttatgaa ttacatccgg
     2341 ggcgggttac cgcagctggg agaggctatt atcatgcatt ttcccgccta ttttatggtg
     2401 atccttctgg gcctcattca gaccacaata cgcagggctt atgccgcagc tgaacgtgaa
     2461 cggcagcatt ccgaaaaatt gcttgaaaac atacttccca ggaagatcat aaatatattg
     2521 aaaaccaatc ccgacactat agccgaaaag tacgatcatt gttcgatctt atttgttgat
     2581 gtggttgatt ttactccatt tgccgaaaaa tcagccccgg aggaagtggt taatattctt
     2641 aatgaaattt ttacactttt tgaccgcctg accgggaaat ataagcttga aaaaatcaaa
     2701 actattgggg atgcttatat ggtggtaagt ggtgtgcccg aaccaaggcc ggatcacagc
     2761 gaagctattg cggatatggc gctggatatg ctccgggttg ttgatgagta taatatcagg
     2821 agcggcaatc atttcagggt caggctgggt attaattcag gtcctgctat tgccggtgtc
     2881 atcggcaaaa tgaaatttgc ttatgatatc tggggcgata cggtgaatac ggccagcaga
     2941 atggaatcgc tgggtgtgcc gggtagaata caaactaccg aggtaaccta tgaaatgctg
     3001 aaaaataaat ttaacttcga ataccgtgat gtcctcgatg tgaaaggtaa agggaagctt
     3061 aaaacgtatt ttcttgaaag caggaaaatg gcttaataag cccggaacga tttgaaagga
     3121 gatgctataa agcgagttgt cagcaggcac agaaaaaaat cagtttcttt gtgatatgaa
     3181 gataaagcta ttattgaaaa tagacgtatc tttgctacag caataaaaaa tattttatgg
     3241 aagattttct cgatatacta aaatacacaa ttccttcggg tgtagtgttt gctacggcgt
     3301 tttttatttt acgtgctttt ttgcaaaacg gaacacaaaa aaaactattg gaactaaagc
     3361 tgtccaacca aaatgtgatt acgcccgtgc gcctgcaggc gtacgaaaga gtgatcctgc
     3421 tccttgagag gatttctccg tcaagtcttg ttttgagggt tgcccggcca ggtatgtcgg
     3481 cggttgagtt acaggcggcg ctgctgcaaa cggtgcgtga tgaatttgag cacaatctgt
     3541 cgcagcaaat ctatatgtca ggcactgcct gggatatgac acgcaatgca aaagaggaga
     3601 tgataaaaat tatcaacctg gcttcaaccc gtctcaacga tgaggctacg gctgccgacc
     3661 tgggctctgt tatactggag ttggccatgt cgcaggataa actacctgta aatatggcta
     3721 ttgattatat aaaaaaggaa gcccgtcagc tatattagtc cggacaggag tgcctgactt
     3781 atcagtcaat gttcagccgg gttttgatgg gtgccggcag cacatctttg tgtaccataa
     3841 tggctatggt tttcagacgg acataatttt cagacaagta ccaataccct tcattgcctt
     3901 ttttttctcc ccatgaattt tttaccaaaa aatattttgc tccgtctttt gaggcagcaa
     3961 ggcctgtgat gtgcatcagg tgatcgtcgg taacggtaaa atcctcgaaa gcctgctgcc
     4021 tggtttcctg gctaaccttt tctttttcgt tgccggtttc ggccacgccg ccgttattcg
     4081 caaaactttt tttgtcgctt acatcgccgt cccataccag ggtgtatcct ttgttcaggg
     4141 agttttccat gacagccatc aggtcttcga gagggaggtt ataatatttt tcaaacatcc
     4201 agttgtcagg tatttcaaga acaaaagatt catagtaggg gtggtgcgaa aaagaagtca
     4261 attctacgta atcatcggcg ttgaggccta agcttgccgc aaaactctcc ggcgaataag
     4321 tatttccgtt atacgaaaat tccgatggaa ttggccccaa atggatatcc atgatgctgc
     4381 ccagcattaa ttcccatttt ttgtctgcgc ttccttcagc tttttttact accgaatcca
     4441 tgatatgttc aagtgcttca tccataagca actgctcgtg gtttttttta ccgttcaggc
     4501 cactatacac ggtttcgggc atagcaccaa attttttaaa cagctgagta atatcgtggg
     4561 cttgcccgcc gggcccgaaa ttgctgtgcc cctgaaagtg ataatagtta ttggccttaa
     4621 tgggataggc catgcgtaca taatacattt ccgacaggtc gtattcgcct ttgttcatgc
     4681 gcaacaactc gctttcaaaa aatgacaggg tggcaaaact ccagcaggta ttggacatgg
     4741 cctggttttt cacaggagtg gttttcaact cgatggtttt ttcaattttt tctatttggg
     4801 catatattgt caaacaaacc aatgacaaaa tacacgatag gatatgtttt ttcataagcc
     4861 gcttttattt tgcaaattta aaatttttaa cttcgtacag gtctttgcaa aataaaagtg
     4921 attatttatg atggatatcg aagcattgtt gaaagaattt tcttttcgca cctcgcgaag
     4981 ttcaggcaag ggcgggcaac acgttaataa ggtttctaca aaagtggaag tgatatttaa
     5041 tgtggcaaat tctatgttgc tttcaaatga tgagaaagag aagatattgg caggggctgg
     5101 caaacacggg agagacgacg gcaccgtgtt tatgatctgc agcgaaggac gttcgcagta
     5161 tctcaacaaa aagaaagcta ttgagaaaat tatccggctc ctcgaaaaat gcctggcacc
     5221 agtcaagagc agggtgccaa ctgcagttcc ggatgaagaa aaagaaaagc gtatggccga
     5281 aaaaaagaaa acggccgaaa aaaagcgtca aagaaaaaat aatcctgaag accagtttta
     5341 agccggtatc ctggtttgcc gaagatattt tgtattagtt cagaaaccct ttttccttaa
     5401 gtatcctgat ggtttcctta taaccgattt cgaacatttt tggccctttg gaggtgtcca
     5461 tcatcgaata cttatgtagt tcggcaggtt caacgaagat gtcaaatttc ccgatttttg
     5521 attcgttgac tttgtttatt gccatgtgca ctactctgtc ggctacattg gtcatgccgt
     5581 tgtatttatt ttctggcaat accgggttta cattggcccc gataagaaat tcgcagtcgt
     5641 tttcgatggg ggcaatgggc aggttgtcca acacgccgcc gtccacgtag gtaactccgt
     5701 cgatggctgt gggctggaat actatcggga cagtgcagga agctatcaca tacttggcta
     5761 aatctccctt tgaaatgtat tccacgcagg catcgtcgag gttggtaaca gccataaaca
     5821 acggaatctg gagctcctca aaggttcttg ctttcaggtg ttgaagcata attttaaaca
     5881 aaccggtcat acgcaacagc cctttgttgg gaacaatgaa ttcgagaaat ttataagtgc
     5941 ttttgttggc aaaaactttg aaaatctcgt ccggtttcca tccgtcggca taaaaggcgc
     6001 caacaataga tcctgcgctg gttccggata ccacatccgg gaatatcccg gcttcgttca
     6061 gggcttttat gatgcctgca tgggcaaacc cgcgtaggcc gccaccgcta agcaccagcc
     6121 ctgtcttgta tttgtgtttg gattttacca tctgctgttt ttttagtgct gcaataaaaa
     6181 atttcttgca gcgtaatcaa atcattactt ccacaaaaat actaatcaag tttcaataag
     6241 cagtaaaatc gcagagaatt agcgaaattt ttattatttg gtattagttg aaattcaggg
     6301 cttccgcaat ttggtctacg gccagcatct tccggtcaag tgtatgtttt tcgccattat
     6361 ccatagtcat gacaactttg ttgcccgaag agtctttaag gatttccacc gacgaaatgt
     6421 gtttttttga taagatcaca tagtcggaaa tatggttttt gagcaaggga gaggtctcaa
     6481 gcataatttc ggcagctccg atgatttttg tatcaaacaa tagccttttg ttcgtaattg
     6541 ttattaatcc aaaaaaactt tggccatcat caaaggtata ataaacatcc catgaattta
     6601 ttattgcttc attagtgagt agttttatat ttagatgggt gctcatatta aaatgcaaaa
     6661 taatgtacaa aggtagtaaa aaaatacaca taagtcaagt tttttttaaa gtatgtgtca
     6721 aataattttt agtagattct atcttttgct tgtacgggtt tttaaaagag ttctttatta
     6781 atcacatcga ttatttgtat aaattcgcac aaaaattgat aacaaaaaaa atataaaatg
     6841 aaaaaagtac tgatcttagg tgctggaatg gttgccaatc caataattca gcatttgtta
     6901 agtaaaaata tcaggcttac cgtagccgcg ttgaactatg atgaagctaa gcgtatggta
     6961 ggacaggccg aaaacggaaa agccgttcac tggacagtgg atgaccaggc aggcctcaac
     7021 actatggttg cagagaatga tgttgtggta agtcttattc cgtatgcttt tcacgtaatg
     7081 gttgccaagt gctgtcttaa gcataaaaaa cacctggtaa cggcttcgta tgccaagccc
     7141 gaaatgtatg cacttgatgc ggcagccaaa gaggctggcg tgattttttt gaacgaggtc
     7201 ggtctcgatc ccggtattga ccacatgtcg gccatgcgca tcatcgacca cgtgcatggc
     7261 aaaggcggaa aaattgaagc tttttattcg ctttgcggtg ccctgcctgc ccccgaatcc
     7321 tgtgataatc cgttcaacta taaattttcc tggagtccca agggcgtggt attggctggt
     7381 aataacgacg ctgtttacct gatgaaaggt aagaaagtgg aaatccctac tgccgacttg
     7441 ttcaaaaacc caatgatcgt agattttcct gaggttggcc caatggatgt ttaccccaac
     7501 cgcgactcat tgtcgtacgt ggatatttac ggtattccgg aaacaaaaac catgtttcgc
     7561 gggacttttc gtttcaaagg gtggtgcgaa acgctcgacg taatgaaagc actgaaatgt
     7621 acccttcctg aaaagcatga tttcagcgga aaaacttttg ccggtgctgt tgctttggtc
     7681 aatggattat ccgatgaaaa gaatattaaa aagcaagtgg ccgccctgtt gcatattgat
     7741 gaaaatgctt acgccctgaa cgccatggaa tggttgggat tattcagcaa tacgcctatg
     7801 aaccgtaccg aggattcgat gtatgaaatc acttccgacc tgatgatcga aaaaatgatg
     7861 attacggaaa atgatcggga tatggtggtg atgatgcatg tttttaaggc ggtttacccc
     7921 gatggaacag cggaaatcat taaatcgagg atgctcgact atggtaccct caaaacggat
     7981 acatctattg cacgaaccgt ggctctacct gctgctactg ctgt
//
LOCUS       DAOSFP010000067         7955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7831_length_7955_cov_4.123038, whole genome shotgun sequence.
ACCESSION   DAOSFP010000067 DAOSFP010000000
VERSION     DAOSFP010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7831_length_7955_cov_4.123038"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2385
                     /gene="dnaE"
                     /locus_tag="PLI16_03150"
     CDS             <1..2385
                     /gene="dnaE"
                     /locus_tag="PLI16_03150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOH83582.1"
                     /translation="VVVYCLGITDLDPLRYGLLFERFLNPDRISMPDMDIDFDEDGRE
                     KVINWVVEKYGKSRVAQIITFGTMASKMAIRDVARVENLPLPDADRLAKMVPVGVKDL
                     NEAITSTKELKQAKDSTNPLIAKTIKYAVELEGSVRHTGIHACGVIIGKDDLIDHIPL
                     CTSKDSDLMVTQYEGAHVESVGLLKMDFLGLKTLSIMKDCLENIKHSQGIDFDLNAIP
                     PDDAETFELFSKGNTTAVFQFESDGMKKHLRELKPNRFEDLIAMNALYRPGPMDNIPS
                     YIRRKFGKEQVVYDHPLMEESLKDTYGITVYQEQVMELSRKMGNFTRGEADQLRKAMG
                     KKNISLMNELHPKFLKGCEDNGISAAIVNKVWDDWKAFAQYAFNKSHSACYAITAYRM
                     AYLKAHYQNEFMAAVLSRNVSNIDEITFLIDECKKLGIQVLGPDVNESQIKFTVNSKG
                     EIRFGLAAIKGLGNAAVDTIIDERRANGDFKNIFDFMNRVSLRTVNKKSIEALAMAGA
                     FDSMDIHRAQFFYKSPNEDTTFLEKVIKHITLIQNKQNTLQQSLFGESEDTAIPDPEL
                     PACEPWGTVEKLNHEKNIIGFYMSGHPLDNYKIELKFFTNFTLDKCDNLKAMAGRDIR
                     FGGMIINAGHKINKNNKPFGYFELEDYTGTTKLNLWGENYDKYKHLLEIGKCIFVSGK
                     IDQRGWNNKDKNPDQPIEYEIKVVSIEPLYSLMEKKAKEVTLLVSLDTISPQFISYMK
                     QLVATYPGQVKLKIQIVDEDESIALDMLSRKYKVSCDGFLKELETINGIKYKLN"
     gene            2426..2743
                     /gene="trxA"
                     /locus_tag="PLI16_03155"
     CDS             2426..2743
                     /gene="trxA"
                     /locus_tag="PLI16_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH83583.1"
                     /translation="MAHEITDNNFDQMVSNSDKVVMLDFWAEWCGPCRMIGPIVEELS
                     REYDGKAIIGKVNVDENPGITAKLKIRNIPTLLFFKNGAEVDKIVGAASKASIASKLN
                     SLL"
     gene            2768..3703
                     /locus_tag="PLI16_03160"
     CDS             2768..3703
                     /locus_tag="PLI16_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOH83584.1"
                     /translation="MPVTIDQKLVQSFGNLELIAKQVVEGFITGLHKSPFHGFSVEFS
                     EHRPYNQGESIRHIDWKLFGRSDKLYTKRFEEETNLRCQIIIDNSSSMYYPVTKDISV
                     SNPNKIIFSIYAAASLIYLLRLQRDAVGLSIFSEKVEEHLSAHSTITHQKVIYQSLEK
                     MMHPFIEGGNKKTFAAEALHDIAEMIHKRSLVVIFSDMMESNTETNELFAALQHLKHN
                     KHEIILFHVTDKSKELDFSFDNRPYRFIDMESAEEIKLNPAEIKDRYLEAINHFRNEL
                     KLRCSQYHIDLVEADINQGFHHVLLSYLLKRKRMF"
     gene            complement(3735..4856)
                     /locus_tag="PLI16_03165"
     CDS             complement(3735..4856)
                     /locus_tag="PLI16_03165"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOH83585.1"
                     /translation="MISIAWHSRAFSMFISILFVLLWIWPARLVSQTLTDTLPHNKNA
                     VLEEFTAVRCYYCPLGHDLLDSLLLENPGRIVPVAMYPSNVSGLLTVPYTGSPDLRRT
                     FVNEFFTMPFVHDSVRFFPGAFINRRQWQPARREQSRENWRSCAGAILNEQSPVNIGI
                     SAEYDQSAFQLNIEVEIYFTDSVNDSCALYVFLTEDSLIAEQYNGGVNYVHNHVFREA
                     LCLQWGDTLCSQVNKGTLITRNFVFDNSGSQYAMHHAQVAAYVRNSANEEIITGSRVA
                     VSNLVVTASTNTPQHNTLVLFPDPSDGRFFIIPQGEHDMTSITKVKIFTATGILCYAA
                     DMAVGEGQLLDFSFLRQGLYLVYFETTKDFFAAKLIIDH"
     gene            complement(4876..6111)
                     /gene="pepT"
                     /locus_tag="PLI16_03170"
     CDS             complement(4876..6111)
                     /gene="pepT"
                     /locus_tag="PLI16_03170"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOH83586.1"
                     /translation="MKEKVLERFLRYIKIDTQSNENSETTPSTEKQFALARVLKQELE
                     DLGLQDISLEDKCYLMATLPANTSETIPTIGFIAHMDTSPDMPGQVENPNFVYDYNGG
                     DIVINKEKKLVLSVRDFPELADYIGKTIITTDGNTLLGADDKAGIAEIMTAMEFLVNN
                     PQIKHGTLRVGFTPDEEIGRGVDHFDVKKFNADFAYTLDGGGIGELEYENFNAAGAKV
                     FIQGRNIHPGYAKDKMINAMLVAMEFNALLPANQRPEHTVDYDGFYHIVKMEGTVENA
                     MVQYIIRDHNKVKFELKKKLFADCVEFLKSRYGAEVLKLEIKDQYYNMKEMVLPVFHV
                     VETAQKAMELVGVKPKVVPIRGGTDGARLSYMGLPCPNIFAGGHNFHGKFEYLPVESM
                     EKAVDVILKIIELYSTKTA"
     gene            complement(6171..>7955)
                     /locus_tag="PLI16_03175"
     CDS             complement(6171..>7955)
                     /locus_tag="PLI16_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOH83587.1"
                     /translation="IAVGASAAAKRKDALGQNVIIQSIGASSGVIVAGAVFTIPGLYI
                     LQAKYPEIQVNFWQIFFSSLLGGFLGILFLIPFRKYFVKDMHGKLPFPEATATTEILM
                     TGEKGGKQAKLLILSGLIGGAFDFCFSALRLWSDEFTSRIIPIGETLATKVKMVFKFN
                     VSALIFSFGYLIGLRYALIITMGSLLSWFVLIPLVNEIGAFVAQSGGGINPFEALSAE
                     DIFKAYVRPIGIGAIAMAGIIGIIKSSSVIGSAFKLAFGFGKKTHAEVKVERTQKDLK
                     MSFVMLFILLTLIAIFVFLLFGVKITLVQAVVALLTVLIISFLFTTVAANAIAIVGTN
                     PVSGMTLMTLILSSVILVSVGLKGWQGMVSSLIIGGIVCTALSMAGGFITDLKVGYWL
                     GTTPAKQQSFKFLGTLLSAATVGIVIFILNDAYGFVETAKHTKPMVAPQANAMAAIIE
                     PLMSGSGVSWVLLGVGAIISLIVNWLKVSPLAFALGMFIPLPLNTPLVVGGLLNHWFT
                     KSTKDEKLANARIQRGILISSGFIAGAAIFGVIGALIIFFTDDPNAVNLKVWDNDTGV
                     GAQFTALIAFLALIAYFVWESFRAKKED"
BASE COUNT         2427 a         1827 c         1686 g         2015 t
ORIGIN      
        1 gtggtggttt attgccttgg catcaccgat ctcgacccac ttcgatacgg attgcttttc
       61 gagaggtttc tcaatcccga tcgtatcagc atgcccgata tggatattga ttttgatgaa
      121 gacggccgtg aaaaagtaat caactgggtg gtggaaaaat atggaaaatc gcgggtggca
      181 caaatcatca ccttcgggac catggcctca aagatggcta tcagagacgt tgccagggtg
      241 gaaaatcttc cgctaccgga tgctgacagg ctggcaaaaa tggttcctgt cggagtaaaa
      301 gatttaaacg aggctattac ctccaccaaa gaattaaaac aagctaaaga ctccaccaac
      361 ccgctgatag ccaaaaccat taaatatgcc gtggaactcg aaggatccgt ccggcacacc
      421 gggatacacg cctgcggggt tatcattggc aaggatgacc tgatagacca tattcctttg
      481 tgcacctcca aagattcaga tttgatggtt acccaatacg aaggcgccca cgtggaatct
      541 gtcgggttgc tcaaaatgga tttcctgggc ttgaaaacct tatcaatcat gaaagactgc
      601 ctggaaaaca tcaagcattc ccagggtatt gattttgacc tcaacgctat tcctcccgac
      661 gatgccgaga cctttgaatt gttcagcaaa ggaaatacga cagccgtttt tcagtttgag
      721 tcggacggta tgaaaaagca ccttcgcgaa ctgaaaccca accggtttga agatcttata
      781 gccatgaacg ccctttaccg cccggggcca atggataaca tcccaagtta tatccgccgg
      841 aaattcggca aagaacaagt tgtgtacgat catcccctga tggaggaaag cctcaaagat
      901 acatatggta ttacagtata tcaggaacaa gtcatggaac tatcgcgcaa gatgggaaac
      961 ttcacccgcg gtgaagccga ccagcttcgc aaggccatgg gcaagaaaaa catttccctg
     1021 atgaatgaac tacacccaaa atttctcaaa ggatgcgagg ataatgggat atcggccgct
     1081 atagtaaaca aagtatggga cgattggaaa gcttttgctc aatatgcttt caacaaatcg
     1141 cactcggcat gctatgccat caccgcatac cgcatggctt atctgaaggc acactatcag
     1201 aacgaattta tggctgcggt gctaagccgc aatgtttcca atatcgacga aatcaccttt
     1261 ttgatcgatg aatgtaaaaa gcttggcata caggtacttg gacctgatgt gaacgaaagt
     1321 cagattaaat tcactgtaaa cagtaaaggc gaaattcgct tcggcctggc cgccatcaaa
     1381 ggcctgggaa atgccgccgt tgacactatt attgatgaac gcagagccaa tggggatttt
     1441 aaaaatattt tcgatttcat gaaccgtgtt agtctgcgca ctgtgaataa aaaaagcatc
     1501 gaagcccttg cgatggcggg agccttcgac agtatggaca tccaccgcgc acagtttttt
     1561 tacaaatcgc ccaacgaaga cactaccttt ctggaaaaag tcatcaaaca cataaccctc
     1621 atacaaaaca aacaaaacac cttgcagcaa tcgcttttcg gcgaaagtga agataccgcc
     1681 atccccgacc ccgaacttcc tgcctgcgaa ccgtggggca ccgttgaaaa actcaaccac
     1741 gaaaaaaaca tcatcgggtt ttacatgtca ggccaccctc tggacaacta caaaattgaa
     1801 ttgaagtttt tcaccaattt tacactcgac aaatgcgata atcttaaagc gatggccggc
     1861 agagacatac gctttggcgg catgataata aacgccgggc ataaaataaa caaaaacaac
     1921 aagcccttcg ggtattttga actggaagac tataccggaa ccacaaaact aaatctgtgg
     1981 ggcgagaatt atgacaaata caagcacctg ctcgagattg ggaaatgcat atttgtatcc
     2041 gggaaaatag accagcgcgg ttggaataac aaagataaaa atcccgacca gcccatagaa
     2101 tacgaaatca aagtcgtttc gattgaaccg ctttacagtc ttatggaaaa aaaagctaaa
     2161 gaagtaaccc ttttggtgag tctcgatacg atttccccac agtttatcag ctacatgaaa
     2221 caacttgtag ccacatatcc cggccaggta aaactaaaaa tacaaattgt ggatgaggac
     2281 gaaagtattg cgctggatat gctatcgcgc aagtacaaag ttagctgcga cggattttta
     2341 aaggaactgg aaacaattaa cggcatcaaa tataaactga attgataaat agttattttt
     2401 gtaaaaaatt aaacaaacaa aatttatggc acacgaaatt accgacaaca actttgacca
     2461 aatggtttca aattcagata aagtggttat gcttgacttt tgggctgaat ggtgcggccc
     2521 ctgccggatg attggcccta ttgttgaaga attgagcagg gaatatgatg gaaaggctat
     2581 tattggtaaa gtgaatgtag atgaaaaccc cggtattact gcaaaattaa aaatacggaa
     2641 catccccacc ctgttgtttt ttaaaaatgg cgccgaagtg gataaaatag tcggagccgc
     2701 ttcaaaagca agtattgcaa gcaaactaaa cagtttatta taacattttt gcacagaggc
     2761 aaacaacatg cctgtcacca ttgatcagaa acttgtccaa tcttttggca accttgaatt
     2821 aatagccaaa caggttgtag aaggttttat taccggcttg cataaaagtc cgtttcatgg
     2881 cttttctgta gagttttcgg aacaccgccc ttacaaccag ggagagtcga tacgtcatat
     2941 cgactggaaa ctttttggcc ggtcggataa gctgtacacc aaacgctttg aagaagaaac
     3001 caaccttcgt tgccagataa tcatcgacaa ctcttcttca atgtattacc cggtgacaaa
     3061 agatatttct gtatccaatc ccaataaaat cattttttca atttatgcag cagcctcact
     3121 gatatatctg ttgcggctcc agcgtgatgc cgtcgggttg agcatatttt ccgaaaaagt
     3181 agaggaacat ctgtcggctc actccaccat aacacatcag aaagtcatct accaaagtct
     3241 cgaaaaaatg atgcaccctt ttatagaggg cggaaataaa aagacttttg cagcagaagc
     3301 gctgcacgac attgccgaaa tgatccacaa acgctcgctt gtggttattt ttagcgatat
     3361 gatggaaagc aataccgaaa caaacgaact ttttgctgcg ttgcaacatc tgaaacacaa
     3421 caaacatgag attatcctgt ttcatgttac cgacaaaagc aaagaacttg attttagttt
     3481 cgataacagg ccataccggt ttattgatat ggaaagcgcc gaagaaataa agctaaaccc
     3541 cgcagagatc aaagaccgct atcttgaagc catcaatcat ttccgcaacg aattaaaatt
     3601 acggtgcagt caataccata tcgacctggt agaagccgat atcaaccagg gatttcacca
     3661 tgtactgcta tcctacctgc tgaaaagaaa aagaatgttc tgatgccttt tatttctgct
     3721 ggcaagaacc aaggttaatg atcaatgatt agcttagctg caaaaaaatc ttttgtagtt
     3781 tcaaaataaa ccagatacaa tccctgtctc aaaaaagaaa agtcgagcaa ctgcccttct
     3841 ccgacagcca tatcggcagc ataacacaaa ataccggtag cggtaaaaat tttcaccttc
     3901 gttattgatg tcatatcgtg ttcgccttgc gggattataa aaaatctgcc gtccgaaggg
     3961 tcgggaaaca gcacaagcgt gttatgctgt ggtgtattag tactggccgt aacaacaaga
     4021 ttgctgacag caaccctgct ccctgttatg atttcttcgt tggctgaatt tcgcacgtat
     4081 gcagcaacct gggcatggtg catagcatat tgactccctg aattatcaaa aacaaagttc
     4141 cgggtaatca atgttccttt gtttacctgc gaacataggg tatcgcccca ctgcaggcaa
     4201 agcgcttcgc ggaaaacatg attatgcaca taattaacgc ccccgttgta ttgctctgca
     4261 ataaggctgt cctctgtcag aaacacatat aaggcacaac tgtcatttac ggaatccgta
     4321 aaataaattt ccacctcaat attcaactga aatgctgact gatcatattc ggcgctgatc
     4381 cctatgttta caggcgattg ttcgttaaga atggcgcctg cacatgaccg ccaattttcc
     4441 cttgattgtt cgcgccgggc aggttgccac tgccgtcggt taataaaagc tcccgggaaa
     4501 aaccttaccg aatcatgcac aaaaggcata gtaaaaaatt cgttgacaaa agttcttcgc
     4561 aggtcgggac taccggtata gggcaccgta agcagcccgc tcacattgga aggatacatg
     4621 gccacgggca cgatcctgcc cgggttttcc agcaacaaac tgtcgagaag gtcatgtccc
     4681 aaagggcagt agtaacaacg caccgcggtg aactcctcca gcacggcatt tttattatgt
     4741 ggcagggtat cagttaatgt ttgcgaaacc aatcgtgcag gccatatcca taataacacg
     4801 aacagaatgc tgatgaacat tgaaaaagca cgactatgcc acgcaatgct tatcatgctt
     4861 tatttttttt cagtgttatg cagtctttgt tgaatacagc tcaataattt tcaggatcac
     4921 atcaacagct ttctccatgg attctacagg aaggtattcg aactttccgt gaaaattatg
     4981 gccgccggca aaaatattcg gacatggcag acccatgtat gaaaggcggg cgccatcggt
     5041 gccgccccgt ataggaacaa ccttgggttt tacaccaacc agctccatag ctttttgagc
     5101 tgtttccact acgtggaata ccggcaaaac catttctttc atattatagt actggtcttt
     5161 tatttccagt ttaagcactt cggcaccata acggctcttc agaaactcca cacaatccgc
     5221 aaaaagtttt ttcttcaatt caaatttcac cttattgtga tcgcggataa tatattgtac
     5281 catagcattt tcaacggtgc cttccatttt cacgatatga taaaagccat cataatcaac
     5341 ggtgtgttcg ggccgttgat tggcaggcag caacgcatta aattccatgg ccaccagcat
     5401 ggcattaatc attttatcct tagcataacc gggatgtatg ttgcgtccct ggataaaaac
     5461 cttggcgccg gctgcgttga aattttcgta ttccagctca ccaattcctc ctccatcgag
     5521 ggtataggca aaatcagcat tgaatttttt cacatcaaaa tggtcaacac cacgtccgat
     5581 ttcttcatcg ggagtaaagc caacacgcag ggttccatgc tttatctgag gattgttcac
     5641 caaaaattcc atggcggtca ttatttcggc tataccagct ttgtcgtcgg cacccagcaa
     5701 ggtatttcca tcggtagtta tgatggtttt gccgatgtaa tcggcaagtt ccgggaagtc
     5761 cctaactgac agcaccagtt ttttttcctt attgataacg atatccccgc cgttgtaatc
     5821 ataaacaaag ttcgggtttt cgacctggcc gggcatatcg ggactggtgt ccatgtgtgc
     5881 aataaatcca attgttggta tggtctcgct ggtgtttgcg ggtagcgttg ccatcaggta
     5941 gcacttgtcc tcaagtgaaa tatcctgaag tccgaggtct tccaactctt gtttcagtac
     6001 cctggccagt gcaaattgtt tttcggtgct gggggtggtt tccgaatttt cattcgactg
     6061 ggtatcaatt tttatataac gtaaaaaacg ttccagaact ttttctttca tattatcaga
     6121 ctttttcaat taatcaaaaa caaacttttg ttaaaatgtt gacggaccaa ttaatcttcc
     6181 ttttttgccc tgaatgattc ccatacaaaa tacgctatca gagccaggaa ggcaatcaaa
     6241 gccgtaaact gagcgcccac gccggtatcg ttgtcccata ctttaaggtt aacagcgttg
     6301 gggtcatcgg taaagaagat tatcaacgcg ccgataactc caaaaatagc cgcgccggca
     6361 ataaaccctg aagatatcaa aatcccgcgc tggatacggg cgttggcaag tttttcatct
     6421 ttcgtagatt tggtgaacca gtggttcagc aatccgccca ccaccagagg agtgtttaac
     6481 ggtaaaggaa taaacatacc cagtgcaaag gcaagcgggg aaactttcag ccagtttaca
     6541 atcagtgaaa taatggcgcc tacgcccaaa agcacccagc tgacaccgga tccggacatc
     6601 aatggttcga tgatggctgc catcgcgttg gcctggggtg ctaccatggg ttttgtatgc
     6661 ttcgctgttt ctacaaaacc ataagcatca ttaagaataa aaatcacaat gccaacggtt
     6721 gctgcagaaa gcaaggtgcc gaggaatttg aatgattgct gtttggcagg agtagtgccc
     6781 agccagtaac ctactttcag gtcggtgatg aagcctccag ccatggataa ggctgtacag
     6841 acaatgcctc cgataataag tgagctcacc atgccctgcc atcctttcag gcccacggaa
     6901 accagaatga ccgaagacaa aataagtgtc atcagcgtca tgcccgaaac agggttggtt
     6961 ccgacaatag cgatggcatt ggctgcgaca gtggtaaaca ggaaagaaat aatcaatacg
     7021 gtcaacaagg caacaacagc ttgtacaaga gtaatcttaa caccaaacag caggaagaca
     7081 aagattgcaa taagtgttaa caagataaac aacatcacaa aagacatttt gaggtctttc
     7141 tgtgtgcgct ctaccttaac ctcggcatga gtttttttgc cgaagccaaa tgccagcttg
     7201 aacgcacttc caataactga cgaggattta atgattccga taattccggc cattgcaatt
     7261 gcgccaatgc ctatagggcg aacataggcc ttaaaaatat cttcagccga caaggcctca
     7321 aaaggattta tcccgccgcc gctctgcgca acaaaggcgc caatttcgtt gaccagcgga
     7381 atcaggacaa accacgacaa cagggatccc atagtgatga tgagggcata acgtaagcca
     7441 atgaggtatc caaaactaaa aataagggcg ctgacattaa atttgaaaac catttttact
     7501 ttggtggcaa gagtctctcc gatagggatt atgcgacttg taaactcatc gctccatagc
     7561 ctgagagccg aaaaacaaaa atcgaaggca ccgcctataa ggccgctaag aattaacagt
     7621 tttgcctgct tgccgccttt ttctcccgtc ataagtattt cagtggtagc cgtagcttcc
     7681 ggaaaaggaa gttttccatg catgtctttc acaaaatact tgcggaaagg gattaaaaaa
     7741 agtatcccca gaaaacctcc cagcagcgac gagaaaaata tctgccagaa attaacttgt
     7801 atttcaggat attttgcctg caggatatat aggccgggaa tggtaaaaac ggcccctgca
     7861 acaataacac ctgagcttgc gccaatagat tgaataatta cattttggcc cagtgcgtct
     7921 tttctttttg ccgcagccga cgcgccaaca gcgat
//
LOCUS       DAOSFP010000068         7921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7881_length_7921_cov_3.701373, whole genome shotgun sequence.
ACCESSION   DAOSFP010000068 DAOSFP010000000
VERSION     DAOSFP010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7921
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7881_length_7921_cov_3.701373"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2293)
                     /locus_tag="PLI16_03180"
     CDS             complement(158..2293)
                     /locus_tag="PLI16_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOH83588.1"
                     /translation="MNHAEKISKGLGLQQQQVENTIYLLDNGATVPFIARYRKELTGS
                     LDEVQISDIRDMNHQLIELDKRRTTILESVREQEKLTPELEKSIMEAQSMSELEDLYL
                     PYRPKRKTRATIARTKGLEPLATIILKQDNTDIDLKAMEFVDEEKGVQSVDEAIVGAC
                     DIIAEWINEDNQIRSRIRILFEKESVISSRVVKGKEIEGRNYEMYYEMNEPLVKSPSH
                     RVLAMFRGETEGFLKISIEPAAEKALAIIERNVIKARNKASDLVIKAIADSYKRLLQP
                     SMETEMRQLAKERADNEAIRVFAGNLKQLLLSPPLGQKTVLAIDPGFRTGCKVVVLDK
                     NGKLLHNETIYPHPPHNEMKQAINKIESLVDAYKIEAIAIGNGTAGRETENFVRKIKF
                     KKDVIAIVVNESGASVYSASAVAREEFPDYDVTVRGSVSIGRRLMDPLAELVKIDPKS
                     IGVGQYQHDVNQNALQKSLEDVVVSCVNSVGVDLNTASKELLTYVSGVGPALAKNIVE
                     FRNKNGSFSGRGGLKKVPRFGEKAFEQAAGFLRITNAENPLDASAVHPESYEIVNKMA
                     YTLNCTVPDLMKDESLRKQIKLQEFITEKAGLPTLNDIMQELAKPGRDPRQKFDLFEF
                     DKNIHDIKDLIPGMELPGIVTNITAFGAFVDIGVHQDGLVHISQMADRFITNPNEVVK
                     LNQKVRVKVVSVDADRKRIQLSMKGVTSK"
     gene            2524..3582
                     /locus_tag="PLI16_03185"
     CDS             2524..3582
                     /locus_tag="PLI16_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83589.1"
                     /translation="MKCKLFLTFDHELPLGGLRTSARDALFDPTDKVLDIAEQTGVRV
                     TLFTDILCAYRFNEWDKNNFFYPYEDQLHKALCNDHDVQLHLHPHWLTTAFDGHTFSP
                     SKDFSLADFMGHQEWNIGKIIKHGVNFLTELCSVPLSDYQCLAFRAGGYHLDPGSAEI
                     FKNLTAQGIRYDSSVCKNYRFSSGLSTIDFGGMPKKPNWFIDAGGNYKQDAGTGILEI
                     PIAGIPKTIFEIPTRFKLKKYASRAPINHGNQIHAGRPATLKNKITQLFSSRMLSFDN
                     YTFSADYLMRILDHCLRKYRMSDEVLLCAIGHPKTMGEYSMGMLKEFILKVQKKYPRQ
                     VEFYTYRQLFGEQKNQRP"
     gene            complement(3602..4909)
                     /locus_tag="PLI16_03190"
     CDS             complement(3602..4909)
                     /locus_tag="PLI16_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH83590.1"
                     /translation="MLQVYSSQISPRVTFIFDLIFKDILGLEYYLTDNSEAFQNFTGP
                     RLSYSGSRISNEFFIEAHGLLFENDVKPFSIHVSTWNGTKIFFETSAGSDMPFDVFAA
                     SFWLVTRYEEYLQSSRDQHNRFMAAESLAFKNDFLQKPVVNSWAAMLKYLLLKQFPGL
                     VFTPPEYSYISTFDLDNVYCYKGKSWWRNAGGAVKSLMRMDFAGIAERIRVLSGNMSD
                     PYDQYEFHRSLQEQYGFPVIYFLLYAKKSRFDGAVSPASKAYQALIETLKKYAETGIH
                     PSYYTVENPELLQSEVFRLSKALKEPVTKSRQHFLRLSFPATYQNLVKSGITEDYSMG
                     YQSHPGFRAGICTPYFFYDLETEAATSLKIFPFCIMDSMLYDYMKLSPAEAWQHIQNL
                     VGEVKKINGTLITIWHDRTFCNTGKYKGWKELFAETVKLAYKQ"
     gene            complement(4893..5762)
                     /locus_tag="PLI16_03195"
     CDS             complement(4893..5762)
                     /locus_tag="PLI16_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOH83591.1"
                     /translation="MVEQHIAYTKIIDGLDIHPGDVLFVTSDISRLAFHTIRAEGSFD
                     ANLFIDSIQQKLGSEGTLLFPAYNFLLESGDKFDAKKTSPFLCGALSLTAWQRHDFRR
                     TKHPLHSFMVWGKYSGQLCALENKSSFGNDSPFAFMHRQKARMLCIDIDLWGSFTFIH
                     YIEQMEKVGYRFRKKILIDYTDESGATSTNDYCVFKKIPGIEMVFHRMEKILKEQQVF
                     TDVYVNKSLFRLIPLDDNTFSFFINDIRYNKGRNIARFNAVYFLKDIARPVLKKLRFF
                     TPLNEKISDASGI"
     gene            complement(5762..6256)
                     /locus_tag="PLI16_03200"
     CDS             complement(5762..6256)
                     /locus_tag="PLI16_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH83592.1"
                     /translation="MNDITIHAVTEKDLDIIARVKELFTAMYHEYAGMGLSITLPGNS
                     AALWMNSVLPALNRMNMLYVALVEGEVAGFIFGYFNFTRDYLGATKTGVISETYVVPH
                     LRSKGVGRILLGAMEKWFIEKGIATVELQTLYRNEKAVAFWEKAGYTKESIHFRKLSL
                     THGN"
     gene            complement(6253..6519)
                     /locus_tag="PLI16_03205"
     CDS             complement(6253..6519)
                     /locus_tag="PLI16_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH83593.1"
                     /translation="METKENEIYGFIVTRLAEKLNRVGFSEKDLTPGFNLTKSGLLDS
                     MAFVNLVADIEKNYNIEVDFEEAFQNPDFTSVSGLIAVVEKQIK"
     gene            complement(6519..>7921)
                     /locus_tag="PLI16_03210"
     CDS             complement(6519..>7921)
                     /locus_tag="PLI16_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH83594.1"
                     /translation="SGILGTLLAGKAYVPLNHKFPTERTAYMTGFAGVDTYIVGQECY
                     EYFVSLLSKIPQKITAIFPETVNLHTLREEYPQHTFYDSNDVHAVQKNSIHEGGTTDG
                     TAYLLFTSGSTGKPKAVPISNRNATAYIDYMFGLFEFNHEDRFYQASDMTFDLSVQDI
                     FLCWKAGACLCAPKDNTLAAIPRFIKDKKLTVWLFVPSASLLFSKMRLLKPNAFPSLR
                     YSMFCGEALPETTAAAWQAAAPNSVVVNLYGPTETTVAIAHYQWKTNKEENNCINGIV
                     PLGKVFATQQYCVVDESLTPVEKGQVGELCLSGSQVCKDYFRSSEKDEKSFIKIPSLS
                     DETWYRTGDRVMADQSDCLYFFGRNDSELKIKGYRVNLLEIDHAIGSFFSKAVVATVA
                     LADRDNGTNFMVTFISNTDKENKLDEVAVLTMCKQILPHYMLPERVVFIDEMPLNSNG
                     KIDRNALIRKYETTCL"
BASE COUNT         2139 a         1830 c         1692 g         2260 t
ORIGIN      
        1 aaaacaagat tgagcttgac gcgtcaatca tcccggatgg tatttatttc tactcggtag
       61 cttacaaggg tcagaaacta accaaacgaa tggtaattac caaataaagg ataattattg
      121 aaaacaaaaa aaggcagccc gcgggctgcc ttttttatta ctttgatgtt acacctttca
      181 tggaaagttg aattcttttt ctgtccgcat caacacttac gaccttgact ctaactttct
      241 gatttagttt taccacttca ttgggatttg taataaaacg gtcggccatc tggctgatat
      301 gcaccaggcc atcctgatga acaccaatat caacaaaggc gccaaaggcg gtaatgttag
      361 ttactattcc cggcagttcc ataccgggga taaggtcttt tatgtcgtgg atatttttat
      421 caaattcaaa gaggtcgaat ttctgccgcg ggtcacggcc gggttttgcc aattcctgca
      481 tgatatcatt aagagtgggc aggcctgctt tttcggtaat aaactcctgc aattttattt
      541 gttttctcag gctttcgtcc ttcataaggt ccggcacggt acaattaagt gtgtatgcca
      601 tcttatttac tatttcatag ctctcgggat gcacggcact ggcatccagc gggttttcag
      661 cattggtaat acgcaaaaat ccagcagcct gttcaaatgc tttttcaccg aaacgcggca
      721 cttttttcaa tcctccccgg ccgctgaaag agccgttttt attacgaaat tcgacaatat
      781 tttttgccag cgcaggtcct acacccgata cataagtcag cagttctttg ctggccgtat
      841 tcaaatcaac gcccacagaa ttcacgcaac tcaccaccac atcttccagg ctcttctgaa
      901 gggcattttg gtttacatcg tgctggtatt ggccaacgcc aatagatttc gggtcgattt
      961 ttaccagttc ggccagggga tccatcaggc gccgtccaat tgaaacactg cccctgacgg
     1021 taacatcata atcaggaaat tcttcgcggg ctacggcaga cgccgaatac acagaagcgc
     1081 cgctttcatt taccacgatt gcaataacat ctttcttgaa ctttattttc cggacaaaat
     1141 tttcggtttc gcggccggcc gttccgtttc cgatggctat ggcttcaatt ttataggcat
     1201 ccaccaggct ttcaatctta ttgatggctt gtttcatctc attatgcggc ggatgcggat
     1261 aaatagtttc attatgcaat agtttaccat ttttatcgag taccaccacc ttgcaacccg
     1321 tacgaaagcc ggggtctatg gccagcacgg ttttttgccc taagggcggc gacagcaaca
     1381 attgtttgag gtttcccgcg aacacgcgta tggcttcatt atcggcccgt tctttggcca
     1441 gttgtctcat ttctgtctcc atcgaaggct gtaaaagccg tttgtacgaa tcggctatcg
     1501 cctttattac caggtcggaa gctttgttcc gggctttaat cacatttcgt tcgatgatgg
     1561 ccagcgcctt ttcggctgca ggctcaatag aaatttttaa aaaaccctcc gtttcgccgc
     1621 ggaacatggc caatacgcgg tgtgaaggcg acttgacgag gggttcattc atttcataat
     1681 acatctcgta attcctgcct tctatttcct taccctttac caccctggaa ctaattaccg
     1741 attccttttc aaaaagtatc ctgatccgtg accttatctg attatcctcg ttaatccatt
     1801 cagcgatgat gtcgcaagcc ccgacaatag cttcgtccac agattggaca cctttttctt
     1861 cgtcaacaaa ttccatagct ttcaggtcga tgtcggtatt atcctgcttt agaattatgg
     1921 tagccagagg ctccaggcct ttagtccggg caatggtggc gcgggtttta cgtttcggcc
     1981 tatagggcag gtacaggtct tccaattccg acatgctttg tgcttccatg atggattttt
     2041 ccagctccgg ggtgagcttt tcctgttccc tgacggattc gaggatagta gtacggcgtt
     2101 tatcaagttc gataagctgg tggttcatat cgcggatatc gcttatttgc acctcgtcca
     2161 gcgaaccggt aagttcttta cggtaacggg caataaaagg cactgtggcg ccattatcga
     2221 gcagataaat ggtgttttcc acctgctgtt gttgcaagcc caggcctttt gaaatttttt
     2281 cggcgtgatt cataagaaat gtttgacttc aaaattaaaa aaattattac gaacatacga
     2341 gcattttcat ttaaggacaa ttcataaggg agaagttccg aagcccggct tgtgcctgga
     2401 gcataccgct ccaaataacc atggcacaca gaaagaaatg tatgtaattt aataaagtat
     2461 agttaacttt gtagataatt gaaccggtga ttattggcga gcggctgaat aaaaatcaaa
     2521 gcaatgaagt gtaaactgtt tttgactttc gatcacgaat tacctttggg aggcttaaga
     2581 acttcggcaa gggatgcttt gtttgaccct accgacaagg tgctggacat cgcagaacag
     2641 acgggtgtcc gtgttacctt gtttaccgat attttgtgtg cctacaggtt caacgagtgg
     2701 gataaaaaca atttttttta tccttacgaa gaccagcttc ataaagccct gtgcaacgat
     2761 cacgacgtgc agcttcacct gcacccccac tggctaacaa cggcgtttga cggacacact
     2821 ttttcaccat ccaaagattt cagcctggca gattttatgg ggcatcagga gtggaacatc
     2881 gggaaaatta ttaaacacgg agtgaatttc cttacggaac tatgttctgt tccgctgagc
     2941 gattaccaat gcctggcgtt tcgtgcaggc gggtaccatc tcgatcccgg cagtgccgaa
     3001 atttttaaaa accttacggc acaaggaatc cgctatgaca gctcggtttg taaaaactac
     3061 aggttcagtt ccggattatc aactattgat ttcgggggca tgcccaaaaa gcctaattgg
     3121 tttattgacg ccgggggcaa ctataaacag gatgccggta cgggaatcct ggagatacct
     3181 atcgccggca taccaaagac aatttttgaa atacccacgc ggttcaagtt aaaaaaatat
     3241 gcttcacgcg ccccaatcaa tcacgggaat cagatacatg cggggaggcc ggctacgctg
     3301 aagaacaaaa tcacgcagtt gttttcgagc cgaatgttaa gtttcgacaa ttatactttt
     3361 tcggcagatt accttatgag aatactggat cattgcttac ggaaataccg tatgtctgat
     3421 gaagtgctgc tatgcgctat cggccatccc aaaaccatgg gagaatattc catggggatg
     3481 ctgaaagaat ttattttaaa agttcaaaaa aaatacccca gacaggttga gttttatacg
     3541 tatcgccaac ttttcgggga gcaaaaaaat caacgaccct gaaaataaca ggcaccaata
     3601 cttactgctt ataagccagc ttgactgttt ctgcaaataa ttctttccac cccttgtact
     3661 tgccggtatt gcagaatgtg cggtcgtgcc atatcgtgat cagtgtccca tttatctttt
     3721 tcacttcgcc taccaggttt tggatatgct gccatgcttc agcgggactg agtttcatgt
     3781 agtcgtacag cattgaatcc atgatacaga aagggaaaat tttaagactg gtggctgctt
     3841 cggtttccag gtcatagaaa aaatatgggg tgcaaatacc tgcacggaat ccggggtgcg
     3901 actggtatcc catggaataa tcctccgtga tgccggactt caccaggttt tggtaagtag
     3961 ccggaaaaga cagcctgaga aaatgctgac gactttttgt aacgggttct ttcaaggctt
     4021 tggacaaccg aaacacttcg gactgcaaca actcggggtt ttcaacggta taatacgatg
     4081 gatgaatacc tgtttcagca tattttttta aggtttcgat aagggcctga tatgccttac
     4141 tggcgggaga tacagcccca tcaaaacggg attttttagc gtaaagcaaa aaataaatta
     4201 ccggaaaccc atattgctcc tgtaaactcc ggtgaaattc gtattgatcg taaggatctg
     4261 acatgttacc ggataacacc cttatccgct cagcaatccc agcaaaatcc attctcatga
     4321 gcgatttgac agctccgccg gcgtttcgcc accagctctt tcctttgtag caatacacat
     4381 tgtcaagatc aaaagtcgaa atataagagt attccggcgg agtaaaaacc aatccgggaa
     4441 actgtttcaa cagcaaatac ttcagcatgg ccgcccaact attcaccacg ggtttttgca
     4501 aaaaatcatt cttaaaggcc agactttcgg cagccataaa acgattgtgc tgatcgcgtg
     4561 aagattgcag gtactcctcg tagcgtgtta ccagccagaa agacgccgca aaaacatcaa
     4621 acggcatatc ggagccggct gaggtttcaa aaaaaatttt agtgccgttc catgtcgaaa
     4681 catgaattga aaaaggcttt acatcatttt caaaaagcaa cccgtgcgct tcaataaaaa
     4741 attcgttgct gatcctggag cccgaataac ttaacctcgg ccccgtaaag ttctgaaacg
     4801 cctcgctgtt gtcggtcagg taatactcca gtcctaaaat atctttgaaa attaaatcaa
     4861 aaataaaagt aacccgcggg cttatttgtg agctatatac ctgaagcatc gcttattttt
     4921 tcatttaaag gggtaaaaaa cctgagtttt ttcaacaccg gccgggctat atctttcaaa
     4981 aaataaacag cattgaaccg ggcaatattc cggcctttgt tgtacctgat atcattgata
     5041 aaaaatgaaa atgtattatc atcaagcgga atcaacctaa acaagctctt gttgacatac
     5101 acatcggtaa aaacctgttg ttcttttaaa attttttcca ttcgatgaaa aaccatttcg
     5161 atgcccggaa tttttttaaa gacacaataa tcgttagttg atgtagcgcc tgactcatct
     5221 gtataatcaa tcaggatttt tttccggaaa cggtagccga ccttctccat ttgttcaatg
     5281 taatgaataa aggtaaaaga gccccacagg tcgatatcta tgcatagcat cctggctttt
     5341 tgccggtgca taaaggcaaa gggcgaatca ttgccgaaag aacttttgtt ttccagcgca
     5401 cagagttgtc ctgaatattt tccccatacc ataaaagagt gaagcggatg tttggtccgc
     5461 cggaagtcgt gacgctgcca tgccgtcaga gacaaggccc cgcaaagaaa ggggctggtt
     5521 tttttagcat caaatttatc acccgattcc agcagaaaat tataagcagg aaacagcagg
     5581 gtcccctcgc tgcccagttt ttgctgtatg ctgtcgataa acaggttggc gtcaaagctc
     5641 ccctcggctc ttatggtatg aaaggcaagc cgtgagatat ccgatgtaac gaataagaca
     5701 tcgcccgggt ggatgtccag cccgtcaata attttcgtgt aagcgatgtg ttgttccacc
     5761 atcagttgcc gtgggttaaa ctaagtttcc tgaagtgaat gctttccttt gtatagcctg
     5821 ctttttccca gaaggcaaca gccttttcgt tgcggtataa cgtctgcaat tccacagtgg
     5881 caatgccttt ttcaatgaac catttttcca tggcgccaag cagtatgcgg cccacacctt
     5941 ttgaccgcag gtgcggcacc acataggttt cagagataac acctgttttt gttgcaccca
     6001 ggtagtctct ggtaaaatta aaatatccaa aaataaatcc cgcaacctcc ccttctacca
     6061 gcgccacata aagcatattc atcctgttga gggcgggcaa caccgaattc atccacaacg
     6121 ctgcactatt gcccggaagt gttattgaca aacccatacc ggcatactca tgatacatcg
     6181 ctgtaaatag ttctttgaca cgggcaatga tgtccaggtc tttttctgtt acggcatgta
     6241 tggtgatatc gttcatttta tttgtttttc cactaccgcg atcaggcctg aaacactagt
     6301 gaaatcggga ttctggaaag cctcttcaaa atccacctca atgttgtaat ttttttcaat
     6361 atcggctacg aggttcacaa aggccataga atcgagcaac ccggacttgg tcaggttgaa
     6421 acccggtgtc aggtcttttt ctgaaaagcc taccctgttt agtttttcgg caaggcgggt
     6481 aacaataaat ccataaattt cattttcttt tgtttccatc acaggcaggt tgtttcatat
     6541 tttcttatca gcgcgtttct atcaattttc ccattgctgt tcagtggcat ttcatcaata
     6601 aatactacac gctccggtaa catataatga ggtaaaattt gtttgcacat agttaaaacg
     6661 gcgacttcgt ccaatttatt ttctttgtcc gtattactta taaaagtaac cataaaattt
     6721 gttccgttgt cacggtcggc cagggccaca gtggcaacaa cggccttgct gaaaaaactg
     6781 ccaatagcat gatcaatttc caacaggttg accctgtaac cttttatttt taattcgctg
     6841 tcgtttcttc cgaaaaaata caagcaatca ctctgatctg ccatgaccct gtcgcccgtc
     6901 ctgtaccagg tttcatcaga cagtgagggt attttaataa aagatttttc gtctttttcg
     6961 ctactgcgga agtagtcttt gcacacctgc gacccgctga gacaaagttc gccaacctgt
     7021 cccttttcca caggagtcag gctttcatct acaacgcagt attgttgggt agcaaaaact
     7081 tttcccaggg gtacgatacc gttgatgcaa ttattttctt ccttgtttgt tttccattgg
     7141 tagtgggcaa tagccactgt cgtttcggta gggccataga ggtttaccac caccgagtta
     7201 ggagcggcag cctgccaggc agcagcggta gtttccggca gggcttcccc gcaaaacatg
     7261 ctgtatcgca aggaggggaa tgcgttgggt ttcagcaacc tcattttgga aaacaacagg
     7321 gacgcggagg gcacaaacag ccatacggta agtttcttgt ctttgataaa acgcggtatg
     7381 gctgccaggg tattatcttt gggggcgcac agacaggcac cggctttcca gcataaaaaa
     7441 atatcctgta cgctgaggtc gaaggtcata tcggaagcct gataaaacct gtcctcatgg
     7501 ttaaattcaa aaagcccgaa catataatcg atataggccg ttgcattcct gttggaaata
     7561 ggcaccgctt taggttttcc ggtacttccg gaggtaaata gcaaataagc cgtaccatcg
     7621 gttgtcccgc cctcatgaat actatttttt tgaacagcgt gaacatcatt gctatcataa
     7681 aaagtatgct gagggtattc ttcccgcaaa gtatgcaaat tcaccgtttc gggaaatata
     7741 gccgttattt tttgaggtat ttttgaaagc aacgaaacaa aatactcata acattcctgg
     7801 ccgacaatat aagtatccac acccgcgaaa ccggtcatat acgctgtgcg ttccgtggga
     7861 aatttatggt taagaggcac ataggccttt cctgccagca gggtgcccag tatgccgcta
     7921 t
//
LOCUS       DAOSFP010000069         7869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7956_length_7869_cov_4.266957, whole genome shotgun sequence.
ACCESSION   DAOSFP010000069 DAOSFP010000000
VERSION     DAOSFP010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_7869_cov_4.266957"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..862)
                     /locus_tag="PLI16_03215"
     CDS             complement(119..862)
                     /locus_tag="PLI16_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOH83595.1"
                     /translation="MRVFGLIGYPLKHSFSEKYFTEKFRREKISDAKYFLFPISSFSE
                     FHSLRQRYSFAGLNVTIPFKQSIISHLDELDENAAIAGAVNTIKFLKKDNLVLTKGYN
                     TDIDGFEALLAACPLPKDVSALILGTGGASKAVAYVLKQKNIAYHFVSRNPDNETVFS
                     YNTLSGKDIAEHELIINTTPLGMFPDIESFPAIPYGSVSKNHLCIDLVYNPEKTVFMA
                     RCEQQQALVVNGLEMLWVQAEKSWEIWNQ"
     gene            complement(865..1557)
                     /locus_tag="PLI16_03220"
     CDS             complement(865..1557)
                     /locus_tag="PLI16_03220"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOH83596.1"
                     /translation="MLILQKIVQAADTAKMTVATEAHTGLTAWIADMATGFIESAGYT
                     GIMVLMMLESMVAPVPSEAVMPFAGFAIYEGTLTWPGVILFSTIGSIIGSLIGYWVGA
                     KGGRPLVEKWGKYLLLDKHDLDITEKWFNKKGDMTVFVCRFIPVVRHVISIPAGMGRM
                     NLMKFSIYTVLGACMWNTILSIAGYYLKENWDQIMKYSHIIDYVIVAVLAIIVIYYGY
                     KLYQNRKKSKQQ"
     gene            complement(1628..3043)
                     /locus_tag="PLI16_03225"
     CDS             complement(1628..3043)
                     /locus_tag="PLI16_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83597.1"
                     /translation="MSEYFDEFEGFDDIDDMEVSSLISRFEQMQQQNDPVFFDVYECE
                     DIIDYYFARENLKQTGQALQYAIKLHPDSDALLLRQAMLHASANESEKALQLLKKVKP
                     DAEEDPEIHFAKAGIYSQLKMYSQAVSEYKKLLDVWDDPGEIYFNIAFEYENAGNYNK
                     ALNYLKIALEKDPSNTNLLYELGYCFEILNRTEESAKFFTNYLKNNPYSDVAWFNLAI
                     AYGNLGLFEKSIDANDYVIAINPKFTSAYYNKAHALNELGRFEEAVQVFEDLIAADEP
                     DALTYYYLGECYEKMKAWDKALENYHRALELDEFMGEAWMGLGNVHDELCNYCKALLY
                     TRKAVDVEPINPEYWYMLGDLLFKMNQKEQALEAYNKVYELDHSNRDILLDISEVLFD
                     SGKADVAILTTSKGIEEQPENISLYYRLAALLYLTGKEKESLVVFEQAVSLDKEGVAE
                     FFDFDTTLKDKAEFIEILNHY"
     gene            3195..4595
                     /gene="glmM"
                     /locus_tag="PLI16_03230"
     CDS             3195..4595
                     /gene="glmM"
                     /locus_tag="PLI16_03230"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOH83598.1"
                     /translation="MPLIKSISGIRGTIGGKAGEGLSPLEVIKFTSAFAAFIAESKSK
                     CKVVVGRDARISGDMVNRLVCGTLMSMGIDVVDIGLTTTPTAEIAVIELKAQGGIIIT
                     ASHNPRNWNALKLLNARGEFLTNQEAADFLKIAEKGDLTYAEVNNLGLYSVNDTLIDL
                     HIEKILKLPLIDTEAIKKARFNITIDCVNSTGGIALPRLLKALGVAEINELYCVPDGK
                     FPHNPEPLPENLGALAESVVKNKSHVGFVVDPDVDRLAIVCEDGEMFGEEYTLVSVAD
                     YVLSQTPGNTVSNLSSTSALRDITGKYGHKYFTSAVGEVNVVEVMKNNQAVIGGEGNG
                     GVIFPPLHYGRDALAGIALFLSHLAKSKCSCSTLRAKYPDYFISKNKIEAGTDVDIYE
                     ILNQIKEKYKSHPVNTSDGVRIDIENGWVHLRRSNTEPIIRIYAESDSMIKAETLAKK
                     LLDDIRELINSNSSDD"
     gene            4635..5759
                     /locus_tag="PLI16_03235"
     CDS             4635..5759
                     /locus_tag="PLI16_03235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOH83599.1"
                     /translation="MIYLDNAATTPLDAEVAETMYRIIKDYYGNPSSSHATGRNSRVL
                     IEESRSLIADLLGVKPSEIYFTSCATEAIATAIQGVVSACGIKKIITTAVEHHAVSHA
                     IDNLVKSAAVQVSYVHIDKNAHLDLQHLEELLNEDEKSFVVIMHANNETGTLFPVQEI
                     SDICKEKRAIFFSDMVQSMGKFENRLSEHSPDITCCSAHKLHGPKGIGFLYLNSRVKI
                     APLICGGGQERNMRSGTENIYGIAGMAKSFEVAYRDMAKNREHISKLKSYFSDKLKLY
                     FPELIFNAGCDHQGLFNILNISVPAAYNNNLLLEKLDMQQIAASGGSACNAGIVKPSH
                     VLTALGADTDLPAIRFSFSKYNTIQELDHVLEILKEILIK"
     gene            complement(5770..7581)
                     /locus_tag="PLI16_03240"
     CDS             complement(5770..7581)
                     /locus_tag="PLI16_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOH83600.1"
                     /translation="MIRLTTLSLVCLVLAWCTKVSAQEVQISTKKETIGNTEYYLHTV
                     NPGQTIYGISKAYTVSMDEIMKSNPELKKGLRAGDVIKIPVLSSNQAKKFISHEVVRG
                     ETLYEISFNYNVKVSEILSCNPGLSEKLKPGSTIQIPVAEKKIKDTVPATLGLYIVRQ
                     GETIYSIARLYATTIDELKKINPGLTETIQVGQQIRLPFGAVIGKTAVKDSAVKKDSV
                     ILIDCGKSGMNNTYRIALLIPFYLDRTGSIDTADGKTPISSHKSLNFIQFYEGVRIAI
                     DSLRQSGLSLMIYVYDVHENTDAQDFVNQKTEMKNMDLIIGPFFMNNFTHFADWAKNR
                     GINIVNPFTKKESALENNPHAFKIVASDSSQAAKVLGFIAETYPGSNVIVVHRNADSS
                     LVRAFDANASAINQGRSLFIYHSINYSGKGFAALSDGLKSDVVNVVITLAQGEAFVSA
                     YIRNLNEAAHKYKIVLFGMPSWEQYPSLDLEYLMNLNLHIFDNYFIDYNKPETADFIK
                     KFRRKYSTEPDYYGYQGYDLMMYFSNALGKYGENFQQCLEQYNPALLETEFVFRKTMS
                     GGYENTGCMIYRYEDYKIINALLNPKKEINLVEKKRH"
     gene            complement(7583..>7869)
                     /locus_tag="PLI16_03245"
     CDS             complement(7583..>7869)
                     /locus_tag="PLI16_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HOH83601.1"
                     /translation="IEGLKSQGLYDKVWQAGAMLLPVQSVGVMGDERTYEHVVALRAV
                     NSTDGMTADWSQLPYEFLAKVSNDIINKVKGVNRVVYDISSKPPATIEWE"
BASE COUNT         2256 a         1765 c         1615 g         2233 t
ORIGIN      
        1 tcgaacccgc gaccccgtgc gtgacaggca cgtattctaa ccaactgaac taccgaacct
       61 gatttgagag tgcaaagata cactattttt gttttcaaca aaacaaaaaa ataaaaaatt
      121 attgattcca gatttcccac gatttttcag cctgcaccca caacatttcc aagccattga
      181 ctaccaaggc ctgctgttgt tcacacctgg ccataaagac ggttttttcg ggattgtaaa
      241 ccaggtcgat gcaaaggtga ttttttgaga cagacccata gggaatagcg ggaaaacttt
      301 caatatcagg aaacatcccc aatggggttg tattaataat cagctcatgc tctgcaatat
      361 cctttccgga aagggtgtta tacgaaaaaa ccgtttcatt gtcagggttt cttgaaacaa
      421 aatgataagc tatgtttttc tgcttcaaaa cataagccac agctttggat gcaccgcccg
      481 tacccagtat cagcgcgctg acatcttttg gcaacggaca tgcggcaagc agggcctcaa
      541 aaccatcaat atcggtatta tagccctttg tcagcaccaa attatctttt ttcaggaatt
      601 ttatggtatt cacagcccct gcaatagccg cattctcatc caattcatcg agatggctta
      661 taatgctttg tttgaaagga atggtaacat tcaaacccgc gaatgaatag cgctgcctca
      721 atgaatgaaa ttcgctgaac gaacttatag gaaaaagaaa atacttagca tcgcttatct
      781 tttcgcgcct gaatttttca gtaaaatact tctctgaaaa ggaatgtttc aagggatagc
      841 cgatgaggcc aaacaccctc ataactattg ttgtttggat tttttacggt tctgatacaa
      901 cttgtaaccg taataaatca ctattatagc gagcacagcc acaatcacat aatcaataat
      961 atgactgtat ttcattatct gatcccagtt ttctttcagg taataaccgg caatggaaag
     1021 tatggtgttc cacatacagg cgcccagcac ggtataaatt gaaaacttca tcaggttcat
     1081 ccgtcccatg cctgctggga tagaaataac gtgccgcacc acaggaataa aacggcagac
     1141 aaagacggtc atgtcgcctt ttttgttaaa ccacttctcg gtgatatcca ggtcgtgttt
     1201 atcgagcaac aaatattttc cccatttttc caccaacggt ctgcctcctt tagcgcccac
     1261 ccaataacct atcaatgagc caattatact tccaatagta ctaaaaagaa taactccggg
     1321 ccaggtaagt gtgccttcgt agatagcgaa tcctgcaaag ggcatcacgg cttcgctggg
     1381 cacaggagcc accatacttt caagcatcat aagcaccata atgccagtgt acccggcaga
     1441 ttcaataaat cctgtagcca tatctgctat ccaggcggtt aggcccgtat gcgcttcggt
     1501 ggccacggtc attttggcag tatcagcggc ctgaacaatc ttttgtaata ttagcataaa
     1561 ataatttttc gcaaaattag gttttttctg ctaatactgt ataacaaaaa aatgacatca
     1621 aaatctctta ataatgattg agaatttcaa taaattcggc cttgtccttt aaggtggtat
     1681 cgaaatcgaa aaattcggca accccttctt tatcaagaga caccgcctgt tcgaacacca
     1741 cgagtgattc tttttctttt cctgtcaggt acaacagtgc ggccaaacga taatacaaag
     1801 aaatattttc gggttgctct tcaatgccct tgcttgttgt aagaatagct acatcagcct
     1861 tacccgagtc aaacaacact tccgagatat ccaataaaat atcgcggttg ctgtggtcca
     1921 actcataaac cttattataa gcttccagtg cttgttcttt ctgattcatt ttgaacaaca
     1981 ggtcgccaag catataccaa tattccgggt ttattggctc tacatcaaca gctttgcggg
     2041 tgtaaagcaa tgccttacaa taattacaca actcgtcgtg aacatttccc agacccatcc
     2101 aggcttcacc cataaattca tccagttcca gtgcgcggtg ataattttcg agggccttgt
     2161 cccatgcttt cattttttcg taacattcgc ccaggtaata gtaagtcagg gcatcgggtt
     2221 cgtcagcggc aataaggtct tcgaaaacct gcacggcttc ttcaaagcgt cccagctcat
     2281 tcagcgcatg cgctttatta taatatgctg acgtaaactt gggattgatg gcaatcacat
     2341 aatcgttggc atcaatagat ttctcaaaca atcccaggtt accataggca atggcaaggt
     2401 taaaccaggc cacatccgaa tagggattgt ttttaaggta gtttgtaaaa aacttggccg
     2461 attcttcagt acggttcaat atctcgaaac aataccccag ttcatagagg aggttggtat
     2521 ttgacgggtc tttctccagg gcaattttca gataattcag cgctttgttg taattaccag
     2581 cattctcata ttcaaaggca atgttgaaat atatttcgcc gggatcatcc catacatcca
     2641 atagtttttt atattcgcta accgcctgtg agtacatttt caactgactg taaatgcctg
     2701 ctttagcaaa atggatttcc gggtcttcct cagcatcggg cttaactttt ttgagtaatt
     2761 gcagtgcttt ttccgattca ttggcagagg catgcagcat agcctgacgc agcaggagtg
     2821 catcggaatc gggatgcagt ttaatggcat attgcaaagc ctgtccggtt tgcttcaggt
     2881 tttcacgggc aaaataatag tcaattatat cttcgcattc ataaacatca aaaaaaacag
     2941 ggtcattctg ctgttgcatc tgttcaaaac gagaaatcaa agacgacact tccatatcat
     3001 cgatatcgtc aaaaccctca aattcatcaa aatattccga catcttatct ttcttttttc
     3061 tttagcaaat ttaagaaata atatttaatt aaggcctttt tttcgaaaaa atcatctgac
     3121 aacggccggt taaaaatttt tcggccataa ttacaacatt cgagtaattt tgcataagaa
     3181 taaaattttt ctttatgcca ttaatcaaat ctatttcggg aatccgcggg accatagggg
     3241 gtaaagcagg cgaaggcctc agtccgctgg aagtaataaa atttacaagc gcttttgccg
     3301 cttttattgc tgaatctaaa tcaaaatgca aggtagttgt gggacgcgat gcccgcattt
     3361 cgggcgatat ggtaaatagg ctggtgtgcg ggacgctgat gtcgatgggg atagatgttg
     3421 ttgacatcgg gcttaccacc acgccgacag ccgaaatagc cgtgatagaa ctgaaagccc
     3481 agggcgggat aattattacg gcaagccata accccaggaa ctggaatgct ctgaaactgc
     3541 tcaatgccag gggcgaattt ctcacaaacc aggaagctgc ggattttttg aagatagccg
     3601 aaaaaggcga tctgacctat gccgaggtaa ataaccttgg tttatacagc gtaaacgaca
     3661 cccttatcga cctgcatatt gaaaaaattc tgaaattacc cctgatagat accgaagcca
     3721 tcaaaaaagc ccggtttaat attaccattg attgcgtgaa ttctaccggt ggcatagccc
     3781 ttccccgttt gttaaaagct ctcggcgtag ctgaaattaa cgaattatat tgtgttcctg
     3841 acggaaaatt cccacacaat cccgagccgc tgcctgaaaa tctcggagct ctggccgaga
     3901 gtgtagtaaa aaacaagtcg cacgtaggtt ttgtggtgga tcccgatgtg gatcgcctgg
     3961 ccattgtgtg cgaagatggc gaaatgttcg gcgaagaata caccctggta tctgtggctg
     4021 attacgtgct gagccaaact cccggaaata ctgtttccaa tctctcctcc acttcggccc
     4081 ttcgcgacat tacaggaaaa tatggccata agtattttac ttcggcagtc ggcgaggtaa
     4141 acgtggtgga ggtaatgaaa aataatcagg ctgtcatagg cggcgaaggc aacggcggtg
     4201 tgatatttcc cccattgcat tatggccgtg atgccctggc tggcattgcc ttgtttttat
     4261 cgcatctggc taaatcaaaa tgctcatgtt ctaccctgcg agccaagtat cccgactatt
     4321 ttatctcaaa gaacaaaatc gaagccggaa cagatgtcga tatttatgaa atacttaacc
     4381 agataaaaga aaagtacaaa agccatccgg tgaacacctc tgacggggta cgtatcgata
     4441 ttgaaaacgg atgggtacat ttgcgccggt caaataccga accgattatt cgtatctatg
     4501 ccgaaagcga ttccatgatt aaagcagaaa ccctggctaa aaaactactc gacgatatcc
     4561 gggaacttat taatagcaat tcatccgacg attagtcaat ctataggaaa gcttttttct
     4621 taaaaaaaac gctcatgatt tatctggaca atgctgctac cacgccactg gatgccgaag
     4681 tagcggaaac catgtaccgc ataataaaag attattacgg caatccgtca tcatcacacg
     4741 ccaccgggcg caacagccgg gtgctcatcg aagagtcccg atcgcttatt gctgatttgc
     4801 ttggggttaa gccatcggaa atctatttta cctcctgcgc caccgaggcc attgccaccg
     4861 ctattcaggg ggtggtttcc gcctgtggaa tcaaaaaaat tattaccaca gccgtggaac
     4921 atcatgccgt aagccatgct attgataatc ttgttaaaag cgccgcggta caggtgtctt
     4981 atgtccatat tgataaaaat gcccatctcg acctgcagca cctcgaagag ttgttgaatg
     5041 aagatgaaaa atcttttgta gtaattatgc atgccaacaa cgaaaccggc actctttttc
     5101 ctgttcagga gatttctgat atctgcaaag aaaaacgagc catctttttc tccgatatgg
     5161 ttcagagtat gggaaaattt gaaaacagac tatcggaaca cagcccggat atcacctgct
     5221 gctcggctca caaattgcac ggacccaaag gcataggttt tttgtacctc aactcgcggg
     5281 taaaaattgc ccccctgata tgtggcggag gccaggaacg caacatgcgt tcgggaaccg
     5341 aaaacatcta tggcatcgcc ggtatggcaa aatcatttga ggttgcttac cgtgatatgg
     5401 caaaaaacag ggaacatatc tcaaaattga aatcatactt ttctgacaaa ctgaagttat
     5461 acttccctga gttgatcttt aacgcgggtt gcgaccatca ggggttgttc aatattttaa
     5521 acatttcggt acccgctgca tacaacaaca acctcttact tgagaagttg gatatgcaac
     5581 agattgccgc ctccggagga agtgcctgta acgcgggtat agtaaagcca tctcacgtat
     5641 taacagcatt gggtgctgat acggacttgc ctgccatcag gttttctttc agcaaataca
     5701 acactataca ggaactcgat catgtattgg agattttgaa agagatactg ataaaataaa
     5761 aaagtaaaat cagtgtcttt tcttttccac caggttgatt tcctttttgg gattcagcag
     5821 ggcattaata atcttgtaat cttcgtaccg gtatatcata cagccggtat tctcgtaacc
     5881 acccgacatg gttttccgga atacaaattc cgtttcgagc aacgcaggat tgtattgttc
     5941 cagacattgc tgaaaatttt ctccatattt acccagtgca ttggaaaaat acatcataag
     6001 gtcatagccc tgatacccgt aataatcagg ctccgtcgaa tatttacgcc tgaatttttt
     6061 tataaaatcg gcggtttctg gtttgttgta atcaataaaa taattatcaa agatatgaag
     6121 gttaaggttc attaggtact caagatcgag gctgggatat tgctcccacg agggcattcc
     6181 gaaaagcact attttgtatt tatgagccgc ttcattcaag tttcgtatgt aagccgaaac
     6241 aaatgcttca ccctgagcca gcgtaattac cacattgacg acatcgctct taagcccgtc
     6301 ggataaagca gcaaaccctt tgccggaata gttgatgcta tgatatatga acaggctccg
     6361 gccctgattt atcgcactgg cgttagcatc aaaagccctt accagactgc tgtccgcgtt
     6421 acgatgcacc acaatcacgt tggagccggg ataagtttcc gcaataaaac caagcacttt
     6481 agcggcttgc gaagaatcgg aggcaacaat cttgaaggca tgcggattgt tttccagcgc
     6541 cgactccttt ttggtaaaag gatttacaat attaattcct cgattttttg cccaatcggc
     6601 aaagtgggtg aaattattca tgaaaaacgg gcctataatc agatccatat ttttcatttc
     6661 ggttttctga tttacaaaat cttgtgcatc ggtattttca tgaacatcat atacgtaaat
     6721 cattaaagat aagccgcttt gcctgagtga atctattgca atgcgaaccc cttcgtagaa
     6781 ttgaataaaa ttcagcgatt tgtgggaaga tatcggagtt ttgccatcgg ctgtatcaat
     6841 actgccggtg cggtcgaggt aaaacgggat gagcagggct atacggtaag tattattcat
     6901 tcctgatttg ccacaatcaa tcaggatgac cgaatctttt tttaccgccg aatctttgac
     6961 ggcagtttta ccgataacgg caccaaacgg cagcctgatc tgctggccta cctgtatggt
     7021 ttcggtaagt cccgggttta ttttctttaa ctcatcgata gtggtggcat aaaggcgtgc
     7081 tatagaataa attgtttcgc cctgcctgac aatatacagc cctaaagtgg cgggaactgt
     7141 atctttgatt tttttttcgg ctacaggaat ctggatggtg ctgccgggtt taagcttttc
     7201 tgacaggccc gggttacagc tcaatatttc gctgactttt acattataat taaaggaaat
     7261 ttcgtatagt gtttcgcccc tgacaacttc atgagaaatg aatttttttg cctgattgct
     7321 tgataataca ggaattttga tgacatcacc ggcgcggagc ccttttttta gttcaggatt
     7381 ggatttcatg atctcatcca tgcttacagt gtaagccttg gaaataccat agatggtttg
     7441 tccgggatta acggtatgta agtaatactc agtattgcca atagtttcct tttttgtgct
     7501 gatctgaact tcctgagctg aaacctttgt acaccaggct agcaccaaac aaaccaaaga
     7561 taatgtcgtt aatctgatca ttttattccc attctatcgt tgcaggcggc tttgagctaa
     7621 tatcgtaaac aacccggttg acacctttaa ctttattgat aatgtcgttg gaaacttttg
     7681 ccagaaattc gtaaggcagc tgcgaccagt cggctgtcat gccatcggta gagttgactg
     7741 ccctcagcgc gaccacatgt tcatacgttc tttcgtcgcc catcacgcct accgactgca
     7801 cgggaagcag catggcgccg gcctgccaca ccttgtcgta taatccctgt gatttcaatc
     7861 cttcaataa
//
LOCUS       DAOSFP010000070         7869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7957_length_7869_cov_4.045559, whole genome shotgun sequence.
ACCESSION   DAOSFP010000070 DAOSFP010000000
VERSION     DAOSFP010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_7869_cov_4.045559"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLI16_03250"
     CDS             complement(<1..195)
                     /locus_tag="PLI16_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83602.1"
                     /translation="MKRIIVVFFILFCIVLAGEFFIFSKINTFSHVSDDRYFDAGKRF
                     YKIFNVKIPGQLVFAGEEVPI"
     gene            complement(197..1309)
                     /locus_tag="PLI16_03255"
     CDS             complement(197..1309)
                     /locus_tag="PLI16_03255"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOH83603.1"
                     /translation="MKTSELYLVLAYFFFFIVVLIFSLLINKILLKFSGNLGVRGNIQ
                     CQVRFNSQSKPSFGGISFFIIFLLSITALAFVFSENNLLLNFKLLGIIVATTLGFAMG
                     LFDDAYNTRVSIKLLSQICCGVILIVSGTYISVFESNTLNYILTVLWVVGLMNSINML
                     DNMDAVATLVSIFILLGIFIQNILAHGLSTNLNIVILGIISSLIGFLVYNWPPSKMYM
                     GDTGSQFLGVVLATFSIIFIWDFKEYNLDEIPTKQICAVLMFFALPIIDTATVSIKRI
                     LKGKSPFIGGKDHTTHHLAYLGLSEKQVAALIALLSAVSVALGIICLNIHQWTHGYTA
                     IFVAYFLILFTVLFTIANFNKDKSNPIGTPADQEEK"
     gene            complement(1346..1897)
                     /gene="rfbC"
                     /locus_tag="PLI16_03260"
     CDS             complement(1346..1897)
                     /gene="rfbC"
                     /locus_tag="PLI16_03260"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOH83604.1"
                     /translation="MKKIPTNIPGVLVIQPDVFEDKRGYFFESFNREKFADLDIQYEF
                     VQDNESLSAKDVLRGLHFQKPPFEQGKLVRVIKGAVLDVAVDIRQGSPFYGKWVAQEL
                     TEQNKLMLWIPPGFAHGFVTLAEQTIFSYKCTGFYNKNSEGVIAWNDPDINIHWLCEK
                     PVLSERDKMGVAFKDFVSPFIYK"
     gene            complement(1904..4303)
                     /locus_tag="PLI16_03265"
     CDS             complement(1904..4303)
                     /locus_tag="PLI16_03265"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOH83605.1"
                     /translation="MGFLIMGLLIAFLYLRYTLPLFETSAVIQIDRNDEARSMFGLVG
                     SYEEDNFMAKKVELIRSAVFLQRVTEKLPLSESYFEKGTILDYEQFQTSPYRVEYTVK
                     SDAIYGIPISVEFEDNAKCLLKYEIEKGNEQKLEIPLTSKAVFPEIDVSLANINYSRL
                     EEERRFLSDKKYYFIINNPNQIIPQIVSKLHVEPLNQAGNTIMIRYQDNCAEKATAIV
                     NTIAEEFQSYDIEKQAESANKMLEFIESRMDIIYDTLVKSQEDIINFKKSYGIDSVGT
                     SNLLPSLTSRLNDYQNQLVEIELESNKLQEIEKNIKVASNSDLTRLIVLTSGSDFRGS
                     ISSLLTSLQGMVITREKLLYDLTENSGQIKELDYQIALQKKLIRESIEYLNINLNERK
                     KEIEEKIALYQGKLGVDGESYNPVDLASLERISKINESYYTQLVNKKAEFQLAKAGYV
                     SQNVILQRSGIPTQPISPIRNTIYIIAVALSLFLGMAIIIVRYVLFNNILSLNDIFKY
                     TDISVIGLVPRYPLKIPVSQLIVDEHPRSLIAEALRSIRTNLQFLNNEIGPKIIAFSS
                     TISGEGKTFVAINLAGVIAFSDKKVVVIDLDLRKPKIHIGFGVDNHKGMSTILSHKTE
                     IDECIKHSAIKNLDFITAGPVPPNPSELIFSQDTDEVLKYLQTKYDFIIIDNPPVGLV
                     TDGIKALSIADYPLYVIKANYSKKYYILDLERMVEDSKIKKISIVLNAAEPVNNTYKY
                     GKKYGSKNRGYGYGYGYGYGYGYGYGYGYGMEDESQIAKKHKRKKSMFSRILGFFKVK
                     K"
     gene            complement(4393..5133)
                     /locus_tag="PLI16_03270"
     CDS             complement(4393..5133)
                     /locus_tag="PLI16_03270"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOH83606.1"
                     /translation="MKRYICYLLVFVVFASGCKVFMPYQMLRQGDYPVSVFPDSLRKQ
                     EYRIAPNDILEIRILTNNGEKMVDPVSGDKVGSQLTYLVEYDGQIKLPILNRIPIAGK
                     SISEAEKMLEEKFSVYFNNPFVQIKVTNSRVTIFPGGEGSEPMVVILDNTNTTLFEAL
                     AKIGGIKDGKAHKIKLIRGDLRNPQVYLIDLSTVRGMTEADLVLQANDIIYVEPRAKV
                     PQKLLEALAPFTSLLTSLLMIYQVFVRQ"
     gene            complement(5176..6360)
                     /locus_tag="PLI16_03275"
     CDS             complement(5176..6360)
                     /locus_tag="PLI16_03275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83607.1"
                     /translation="MPRVLRIINRFNLGGPTYNAAYLTRYMCPEFQTLLIGGMKEESE
                     ENSEFIVRNLGIVPITIPEMHRSINPLKDIVALRKICEFIEDYKPDIVHTHASKAGAL
                     GRRAAYKMKVPVIIHTFHGHVFDAYFSGVKSHLYQHIERALARKSTKIIALSPIQRDD
                     LVHKYRICSEDKVRVIPLGFDLDRFRENKEEKRMRFRNDYLLDEDEIAIGIVGRIVPI
                     KNHSLFLEGIKHVVDKSEKRIRAFIVGDGEEKINMMKKCRELGLSTVDFTIEKRKAEV
                     TFTSWIKDVDVVNAGMDIITLTSLNEGTPVSLIEAQASGKPIITTNVGGIENIVIPGH
                     TALLSDNKVPGDYQKKLLSLVEDEQMRNEFSMAGWEYVYQKFSYNRLVTDMKNLYNEL
                     LT"
     gene            complement(6370..>7869)
                     /gene="asnB"
                     /locus_tag="PLI16_03280"
     CDS             complement(6370..>7869)
                     /gene="asnB"
                     /locus_tag="PLI16_03280"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOH83608.1"
                     /translation="LDDTVFIARDRMGVKPLLIYQDNERLCFASEMKALLAMCIPKAL
                     DFVSLLAYLQMNYVPANFSMINQVVKLEPGTYLMIKQGVVEQKRFYSIPYQPRAGNTM
                     DDYETACARVRDLMTDAVEKRMISDVPLGAFLSGGVDSSVIVALASKFTRHLNTFSIG
                     FSDAVFFDETSYAQIVANKFKTNHTVFSLTNDDLFGALYHVLDYLDEPFADSSAIAVY
                     ILSRQTRQHVTVALSGDGADELFGGYLKHEAELRIRNANSIVKLLKYAAPLINLLPQS
                     RDTALFNKFRQAAKYANGLNLSEKERYWRWCSFITGQEAGRMLCGTVNNIEYLRRKES
                     IISNITHKGSLNDVLLADMKHVLVNDMLTKVDLMSMANSLEVRTPFLDYRLVDYVFSL
                     PAAYKIQGNIRKKILQDAFKDILPEEIYHRPKHGFEVPMLSWLRNELFPLINNDLLND
                     DFIVEQNIFEVAETKKLKARLQTNNPGDVHAQVWALIVFQYWWKKYFIN"
BASE COUNT         2294 a         1656 c         1551 g         2368 t
ORIGIN      
        1 gataggaact tcttctcctg caaaaacaag ttgaccggga atcttaacat tgaatatctt
       61 gtaaaatctt tttcccgcat cgaaatacct gtcgtcggag acatgactga aggtgtttat
      121 ttttgaaaag ataaaaaact ctcctgccaa aacgatacaa aataagataa aaaaaaccac
      181 gattatcctc ttcatattat ttttcctcct gatctgccgg ggtgcctata ggattgcttt
      241 tatctttgtt aaaattggca atagtgaaaa gcaccgtgaa tagaatcagg aaatacgcta
      301 caaaaatggc ggtataacca tgagtccatt ggtgtatgtt caggcaaatt attcccaggg
      361 ccacggagac ggcagaaagt aaggcgatga gcgctgccac ttgtttttct gatagtccaa
      421 gatatgccag atggtgtgtt gtatgatcct taccgccaat gaaaggcgat ttgcctttga
      481 gtattctttt tatggaaacg gtagctgtat caataatagg cagggcgaaa aacatcaaaa
      541 cagcgcaaat ttgttttgtc gggatttcgt cgaggttata ttctttaaag tcccagataa
      601 aaataatact aaaggtagcc agtactacgc ccaaaaactg gcttccggtg tcgcccatat
      661 acattttgga gggaggccag ttataaacca ggaaccctat cagggaagaa ataataccca
      721 ggataacaat attcaggttg gttgacaagc catgtgctaa aatattctga ataaaaatgc
      781 cgagtaaaat aaaaattgag acgagtgtgg caaccgcatc catattgtcg agcatgttga
      841 tagaattcat taggccgacg acccaaagta ccgttaaaat ataattcagg gtatttgatt
      901 caaaaacgga tatataagtt ccggaaacta tcaaaattac tccgcaacat atctgggaaa
      961 gaagttttat tgaaacccgg gtgttgtagg cgtcatcaaa aagccccatc gcaaaaccca
     1021 gtgtcgtggc cacgataatg cccaacaatt taaaattcaa caggaggtta ttctcgctaa
     1081 aaacaaaagc aagtgcagtt attgaaagta aaaagataat aaaaaatgaa attcctccga
     1141 aagaaggctt ggactggcta ttaaaccgca cctgacattg aatatttccc cggacgccca
     1201 gatttcccga aaattttaga agaattttat tgatcagcaa agagaaaatg agcaccacaa
     1261 taaaaaagaa gaaataggct aatactaaat aaagctcact agttttcaaa acagaatatt
     1321 ttagcctgca aaaatactaa tattttcatt tgtaaataaa tggagaaacg aaatccttga
     1381 aggccacccc cattttatca cgttcggaga gaacgggctt ttcgcacaac caatgaatat
     1441 tgatatccgg gtcgttccag gcaataacgc cttctgaatt tttattataa aaaccggtac
     1501 atttataaga aaaaatggtc tgttcggcaa gggtcacaaa accgtgtgca aatccaggag
     1561 gaatccacaa cataagcttg ttttgttcgg taagttcctg cgccacccat tttccgtaga
     1621 aaggagatcc ctggcggata tccactgcca catccagcac ggcgcctttg attacccgca
     1681 ccagctttcc ctgttcgaaa ggaggtttct gaaaatgcaa tccacgaagc acatctttgg
     1741 cagacaggga ttcattatcc tgaacaaatt cgtactgtat gtcaaggtct gcaaacttct
     1801 ccctgttaaa tgattcgaaa aaataacctc ttttgtcttc aaaaacatct ggctgaatca
     1861 caagaacacc gggaatattg gtaggtatct ttttcacgta aaattatttt ttaactttaa
     1921 aaaaaccgag aatcctgcta aacatacttt tcttacgttt atgttttttt gctatctggc
     1981 tttcgtcttc cataccataa ccatagccgt aaccatagcc atagccgtaa ccataaccat
     2041 aaccgtaacc gtagcctctg tttttcgagc cgtatttttt accgtatttg taagtattgt
     2101 tgacgggttc agctgcattt aaaacgatgg atattttttt aatcttacta tcttccacca
     2161 tacgttccag gtcgaggatg tagtattttt ttgaataatt agccttgatg acatataaag
     2221 gataatcggc aatcgacaag gctttaatgc catcggtaac cagtcctacg ggcgggttgt
     2281 cgatgattat aaaatcatat tttgtctgga gatatttcag cacctcgtcg gtatcctgac
     2341 taaaaatcaa ttcggaggga ttgggcggca caggccctgc cgtaataaaa tcaagatttt
     2401 ttatggcact gtgttttatg cactcatcta tttcggtttt gtgcgaaagt atcgtgctca
     2461 ttcctttatg gttgtccact ccaaagccaa tatgtatctt gggttttcgc aaatccaggt
     2521 cgattaccac cactttttta tccgaaaaag caatgacacc ggccaggttg atggcaacaa
     2581 atgttttgcc ttcgcctgaa atagtggatg aaaaggcgat aatctttggc cctatttcat
     2641 tgttcaagaa ctgcaggttt gttctgattg accgcagtgc ttcggcaatt aacgaacggg
     2701 ggtgttcgtc aactatcagc tgggatacag gtatttttaa tggataccgc ggcaccaaac
     2761 caataacgga gatgtcggta tatttgaata tatcgttgag gctaaggata ttgttaaaca
     2821 aaacatagcg tacaataata attgccatgc ccaaaaacaa cgacaaggca acggcaataa
     2881 tgtatatggt atttcttatg ggagaaatag gctgggtggg aatgccggac cgctgtaata
     2941 tcacattttg tgaaacatac ccggcttttg caagctgaaa ttcggccttt ttgtttacga
     3001 gttgggtata ataggattcg tttatttttg aaatacgttc gaggcttgcc aaatccacag
     3061 ggttatagct ttcgccgtct acgcccagtt tgccctgata cagggcgatt ttttcttcaa
     3121 tttctttttt tcgttcattc agattgatat tcaggtattc aatactttcc cttatcagtt
     3181 ttttctgcag agcgatctga tagtccaatt cttttatctg tccgctgttt tcggtaaggt
     3241 cataaagcaa cttttcgcgg gtgattacca tcccctgtaa cgacgtaagc agcgaggata
     3301 tagatcctct gaaatcgctg ccggaagtca acactatcaa cctggtcaaa tcggagttgc
     3361 tggccacttt gatatttttt tctatctcct gcagtttatt cgattcaagc tcaatttcca
     3421 ccaactgatt ctgataatcg ttgagccgcg aggtcaggga aggcaacaag ttgctggttc
     3481 caacagaatc gatgccgtat gattttttaa aattgatgat atcttcctgt gatttcacaa
     3541 gggtatcata gataatatcc attctcgact cgataaactc gagcattttg ttggcacttt
     3601 cggcctgttt ttcaatatcg tagctttgga attcttcggc aatagtattg acaatggcgg
     3661 tagctttttc ggcgcaatta tcctgatacc gtatcataat ggtattacct gcctgattta
     3721 aaggctccac atggagtttt gaaacaatct gcggaataat ctggttagga ttattgatta
     3781 tgaaataata ctttttatcg ctaagaaatc tacgttcttc ttcaagacgg gaataattga
     3841 tattggcgag ggaaacatca atttcgggaa aaacagcttt ggatgttagc ggtatttcca
     3901 atttttgttc gttacctttt tcaatttcat atttcagcaa acacttggcg ttatcctcaa
     3961 actctacaga tatcgggatg ccatagattg cgtccgattt gacagtgtac tctacccggt
     4021 acggagatgt ttgaaactgc tcataatcca ggatggtacc tttttcgaaa tatgactcgg
     4081 aaagaggcaa tttttcagtt accctttgaa gaaaaactgc agaacggatt agctcaacct
     4141 tcttggccat aaaattatct tcttcgtatg agccaacaag gccaaacatg cttctggctt
     4201 catcattgcg gtcgatttgg ataacagcgg aagtttcaaa aagcggcagt gtatatcgta
     4261 aatacaaaaa cgcgataagc aggcccatta ttaaaaaccc cacaggaaaa taaaagtttt
     4321 tcctgaaaat atataagaat agctttaagt ctatctcatc atttatcggt gatatttttt
     4381 tctttttggc cattattgtc taacaaaaac ctgataaatc attaataatg aggtcaataa
     4441 ggaggtaaaa ggcgccaatg cttccaacag cttttgaggc actttagccc gtggttcaac
     4501 ataaatgatg tcgttggcct gcaacacaag gtcggcttcg gtcattcccc gcacagttga
     4561 aaggtcgatt aaataaacct gtggatttcg caaatcgccc ctgataagtt ttattttatg
     4621 tgcttttccg tctttgatac ctccaatttt tgccagggct tcaaaaaggg ttgtattggt
     4681 attgtcgaga ataactacca tgggttcgct accttctccg cccggaaaaa ttgtaactcg
     4741 gctattggta acttttatct gaacaaaggg attgttgaaa tacactgaaa atttttcttc
     4801 aagcattttt tcggcttcac ttatagattt cccggcaata ggtatccggt ttaagattgg
     4861 gagttttatc tgtccgtcgt attctaccaa ataagtcaac tgactgccaa ccttatcgcc
     4921 gctcaccggg tcgaccattt tttctccatt attagtcaga attcttattt ccagtatatc
     4981 attaggagca attctatact cttgttttcg gagcgaatcg ggaaaaaccg acacgggata
     5041 atcgccttgc cgcagcatct gataaggcat aaatacctta caaccagaag caaacaccac
     5101 aaaaacaagc aagtagcaga tataacgctt cattattatt tttttacaaa attaatattt
     5161 tattgcttta tttgactatg taagcaattc attatacaaa tttttcatgt cggtaaccaa
     5221 cctgttgtaa gaaaattttt gataaacata ttcccaccca gccatactaa attcatttct
     5281 catttgttca tcctcaacca gcgaaagcaa ttttttctgg taatcgcccg gcactttatt
     5341 gtctgatagc aaggctgtgt gcccgggtat gacaatgttt tcgataccgc cgacattggt
     5401 tgtaataatg ggttttccgg aggcctgggc ttcgattagg ctcaccggtg tgccttcatt
     5461 taacgaagtc agggtaatga tgtccatgcc ggcattcacc acatccacgt ctttaatcca
     5521 ggaagtgaat gttacctccg cttttctttt ttcaatggta aagtcaacgg tgctcagtcc
     5581 caactcacga cattttttca tcatgttaat tttttcttcc ccatcgccaa caataaatgc
     5641 cctgattcgt ttttccgatt tatcgaccac atgcttaatg ccttccaaaa aaaggctatg
     5701 attctttatt ggaactattc tcccaacgat tccaatggca atctcatcct cgtcaaggag
     5761 atagtcattc cgaaaacgca tacgtttttc ctccttgttt tcccggaacc ggtcaaggtc
     5821 aaatcccagg ggaattaccc tcactttgtc ttcgctgcaa atccggtatt tgtgcaccag
     5881 atcatcgcgt tgaataggac tcagtgcaat aattttggtg gattttcggg ccaaagcacg
     5941 ttcaatatgc tgatataaat gtgatttaac accactaaaa taggcatcaa aaacatggcc
     6001 gtgaaaagta tgaattatca ccggaacttt cattttatag gccgccctcc tgcctaatgc
     6061 tcctgctttg gaggcgtggg tatgcacgat atccggcttg taatcttcaa taaattcaca
     6121 gatttttctt aaggcgacaa tatcctttag tggatttatg gagcgatgca tctcgggaat
     6181 tgttatggga acgattccca gattcctgac aataaattcc gaattttctt ccgattcctc
     6241 tttcatgccc cctatcagca aggtctgaaa ttcaggacac atataccggg tcaggtaggc
     6301 cgcattgtaa gtgggcccgc caagattaaa acgattgatt attcgtaaaa ctctcggcat
     6361 ttttttagat tagtttataa aatatttttt ccaccagtat tgaaaaacta tcagggccca
     6421 gacctgcgca tgcacatcgc cggggttgtt agtctgtaaa cgtgctttaa gtttttttgt
     6481 ttcagcaact tcaaaaatat tctgctcaac gataaaatca tcatttaaca gatcgttgtt
     6541 gataagcgga aacaattcat tgcgcaacca cgacagcatg gggacttcaa aaccgtgttt
     6601 cggacggtgg taaatttcct caggcagtat atccttaaat gcgtcctgca agattttttt
     6661 tctgatattc ccctgaatct tatatgcggc aggcaatgaa aaaacgtaat cgaccaggcg
     6721 gtaatccaga aacggcgttc ttacctccag ggaatttgcc atagacatca aatccacttt
     6781 tgtaagcata tcattcacca aaacgtgctt catatcagca agtagaacgt cattcaaact
     6841 tcctttatga gtaatgttcg aaataatact ttctttgcgc ctcaggtatt cgatattatt
     6901 caccgttccg cataacattc gtccggcttc ctgtccggta atgaatgagc accagcgcca
     6961 gtaacgttct ttttcggaca aattcaggcc gttagcgtat ttggcagcct gcctgaattt
     7021 gttgaacaag gcggtatcgc gcgactgcgg aaggaggttt attaatggtg cagcgtattt
     7081 tagaagtttt actatgctgt tggcgtttct gatcctgagt tcggcttcat gtttgaggta
     7141 tccgccgaac aattcgtcgg caccatcacc cgataaggcc accgtaacat gttggcgggt
     7201 ttgcctactt aggatataca ccgctatggc cgacgaatca gcaaaaggct catcaagata
     7261 atccagcaca tgatacaagg cgccaaaaag atcatcattg gtaagggaaa aaacagtgtg
     7321 attggttttg aatttattgg ctacaatttg cgcataggac gtttcgtcaa aaaaaacggc
     7381 atcgctaaag ccaatagaaa atgtgttcag gtgtctagta aactttgatg ccaacgccac
     7441 aataactgaa gaatcgacgc ccccgctgag aaaagcgcct aaaggcacat cggaaatcat
     7501 ccttttttct actgcatccg tcatcagatc ccttaccctg gcacaggcgg tctcgtaatc
     7561 atccatggta ttgccggcac gcggttggta aggtatggaa taaaaccttt tttgttcgac
     7621 cacaccttgt tttatcatca gataagtgcc aggttcgagt ttcaccacct ggtttatcat
     7681 tgaaaaattg gccggtacgt aattcatctg aaggtatgcc aaaagagaaa caaaatcaag
     7741 tgcttttggg atgcacatgg ccagcaacgc tttcatttcg ctggcaaagc agagtctttc
     7801 attatcctgg tatatcagca gcggtttaac acccatacgg tcgcgggcaa taaacacagt
     7861 gtcatccag
//
LOCUS       DAOSFP010000071         7868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7964_length_7868_cov_4.160758, whole genome shotgun sequence.
ACCESSION   DAOSFP010000071 DAOSFP010000000
VERSION     DAOSFP010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_7868_cov_4.160758"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="PLI16_03285"
     CDS             complement(<1..1081)
                     /locus_tag="PLI16_03285"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOH83609.1"
                     /translation="MLQSLEKILTDTRFTEICGKAGIGISHISYSSRACAANSVFVAV
                     KGVNADGHKFIADAVKHGATAIVCEVLPAERDPEVTYVKVPDSSYALGIICDNFFGHP
                     SAKLKLVGVTGTNGKTTIATLLYNAFRQLGYKSGLLSTICYKVNDTVYPATHTTPDPF
                     VINKLLSEMVETGCEFAFMEVSSHAVVQHRIAGLTFAGGIFTNITHDHLDYHKTFESY
                     IEAKKGFFDNLPAAAFAITNTDDRNGGVMIQSTRARRFTYGLRQMADFKCRMIESCID
                     GMQLNIDGTDLWCKLSGTFNAYNITAIYAAAILLGQQKDQVLTVLSGLDPAEGRFESI
                     KDNHNVVAIIDYAHTPDALKNVLSTI"
     gene            complement(1089..3455)
                     /locus_tag="PLI16_03290"
     CDS             complement(1089..3455)
                     /locus_tag="PLI16_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOH83610.1"
                     /translation="MDQKNEKKKSILMRAYGIYFLVWIVAIAIMVKTFSLQFINDDYW
                     KSQATTESTRKEIIEPTRGNIYAENGELMATSIPLFQVNLDLDSTAIHDTVFNKHVDS
                     LGICLSMFFDKTPNNKYKYVNELKKARKKYNRNFLLVRKATYAELQQIKKFPLLRRGK
                     YGGLVITKKEKRIRPIKNCAAMTIGYERLAEYNVDINLKAGKVNKQRFDQYVDTLADC
                     FYSTFNDYNKSYYEKILKETYEKSVSSASIRKRMNVLQLAEIKRFPLIAEDTNRILNI
                     QKLTQSYFVGLEGHYKEQLRGTRGVVYLKRVGSNSWKQISEEAMVEPQSGCDLYTAID
                     VNLQDVAQSALRKSLDTTHADWGCVVLMEVKTGYIKAIANLTKDKEGNYKEMRNYAVE
                     ELYEPGSTMKLASVIAVLEDGKYDTNQIVHTGKIRYPDYKKEVVDSHEDGYGDVSMAK
                     AFEKSSNVGITQITRMAFLSKEKIKKFEEYLRKMGLKDRSGIDLDNEPSPYLPIKYED
                     LTAAAFGYSIKLTPLQVLGLYNAVANNGKYMKPQLVKEIRRKGVVVKKNEPEVLINQI
                     CSDATLQKVRSLLEGVVTRGTARNIRKAPYKVAGKTGTARIWDEVTRGYIDRYIASFC
                     GYFPADDPKYSCIVVEYKMQGGEVYGSQVAAPVFKEIADKVFATRVDIKSRELKMPDT
                     LSAPLLDFAYRQDVEDVYTGLNVNLIFKGGASDWVSTTRSDDYKRIYVNDMSVKNSKI
                     PDVKGMTAKDAVYLLEKAGLDVVVKGAGWVKEQRNNLENKTVELILSI"
     gene            complement(3462..3878)
                     /locus_tag="PLI16_03295"
     CDS             complement(3462..3878)
                     /locus_tag="PLI16_03295"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOH83611.1"
                     /translation="MEKNEFSPAPAEEQKPTQGKKKDHKDKRQNLGSVFRSILNGEIL
                     TREAMLKLMPFGLFLCLLAVLYIANSYNYEKNIRKTTQLRDELIELKDEYITTQSDLT
                     HASQQSEIAFRLDSLGTGIKESVVPPMKIQEDNSIK"
     gene            complement(3881..4774)
                     /gene="rsmH"
                     /locus_tag="PLI16_03300"
     CDS             complement(3881..4774)
                     /gene="rsmH"
                     /locus_tag="PLI16_03300"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOH83612.1"
                     /translation="MYHDPVLLQAAVDGLLIRPGGTYVDVTYGGGGHSKEILKRLQGG
                     RLIAFDQDADVEANRIDDDRLTLLNHNFRYLKNLLQYHRLIPVDGIIADLGVSSHQFD
                     VAARGFSFRFGAPLDMRMNQNQKLSAADILNEYSTGQLMEIFRQYGELSNSSQLANKI
                     AAFRKQQKIITCDDLRSAISGLIEKRHEDKYLAKVFQALRIETNSELDALKEFLMQSL
                     VVLKKSGRLVVISYHSLEDRLVKNFMKAGNFDGIIESDLFGNTDTPFELISRKAITPD
                     EKEISQNKRARSAKLRIAEKK"
     gene            complement(4764..5237)
                     /gene="mraZ"
                     /locus_tag="PLI16_03305"
     CDS             complement(4764..5237)
                     /gene="mraZ"
                     /locus_tag="PLI16_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOH83613.1"
                     /translation="MEKLLGEYDCKIDGKGRLMFPAALKRKLPESSGYRFVLNRGFEP
                     CLTLYPIDTWEKIVHDISQLNLYIKKNRDFLRYFYRGATEVEMDATNRILIPKRLFEY
                     AKIDKDVIVFAYVDRIEVWNREIYEKLLTDAPGDFSDLAELTMGNHPSKPQEDVP"
     gene            5672..6739
                     /gene="lpxK"
                     /locus_tag="PLI16_03310"
     CDS             5672..6739
                     /gene="lpxK"
                     /locus_tag="PLI16_03310"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOH83614.1"
                     /translation="MKRLLKILLLPFSAIYGSITFIRNKFYDWGWLPSQAFDLPLISV
                     GNISTGGTGKSPAVEYLIRLLQKQSISIATLSRGYGRQTRGYILADTGATAATIGDEP
                     HQFFRKFNDVAVAVCEDRVKGIAHLLRRVPAPGVILLDDAFQHRRVKPGLSILLTRYN
                     NIYSHDFVLPAGRLREFRCGAARVDIIIVTKSPEQLPETAKDKIIKSLRPLPNQVVLF
                     SHIEYNDFQPFFKDQCQKYLSSTEDFELLLFTGIADARPLITRLEALCKKLHIMHFGD
                     HHVYSQKDIRTISGKFRNIVTEKKAIVTTEKDFSRLAHQDICNELSDFPVFYIPVEMG
                     FSENDKKIFENKIVDYVAKNQ"
     gene            6720..7532
                     /locus_tag="PLI16_03315"
     CDS             6720..7532
                     /locus_tag="PLI16_03315"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOH83615.1"
                     /translation="MLQKINETVAYIKSKTSFEPSIGIILGTGLGNLANEIKVHSVIP
                     YAEIPNFPVSTVTGHKGQLVFGTLNNINVVAMQGRFHYYEGYAMKEVGFPVRVLKYLG
                     IKTLVLSNASGGVNPGFEVGDIMIITDHINLMGTNPLIGKNDDEIGPRFPDMHVAYNK
                     DLINKALKIAEKNNIHCQQGVYAAVTGPCFETPAEYKYVRTVGADCVGMSTVPEVIVA
                     HHMGLPVFAVSIITDLGVEGKIVEVSHEEVIQAANAAEPKMTTIIKELIATI"
     gene            complement(7654..>7868)
                     /locus_tag="PLI16_03320"
     CDS             complement(7654..>7868)
                     /locus_tag="PLI16_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83616.1"
                     /translation="SLMAFGGLFFGLDVVNNVINGDRPVVRADVTLISAAVVATGGIL
                     QLFTSRKCEVSKDKWRLKIIDQIHVK"
BASE COUNT         2016 a         1822 c         1657 g         2373 t
ORIGIN      
        1 tgatagtgct cagcacattt ttgagggcat cgggcgtatg ggcataatca ataatggcca
       61 ccacattatg gttatctttt atgctttcga agcgtccttc agccggatca agtccgctga
      121 gcacagtgag cacctggtcc ttttgctgtc cgagcagaat agccgcagca tatatagccg
      181 taatattata ggcgttgaaa gtgcccgaca gtttgcacca gagatcggtc ccgtcaatat
      241 ttagttgcat cccgtcgata caactttcta tcatgcggca tttaaaatcg gccatctggc
      301 gcaacccata ggtaaaacgc ctggccctgg tgctttgaat catcaccccg ccgttgcggt
      361 catcggtatt ggtaattgca aaggccgcgg cgggcagatt gtcgaaaaag ccttttttgg
      421 cttcgatgta actttcaaat gtcttgtgat aatccaggtg atcgtgggtg atattggtaa
      481 aaatgccccc ggcaaacgtt aatcctgcta tgcgatgctg aaccaccgcg tgcgaactta
      541 cctccataaa cgcaaattca cagcccgttt ccaccatttc ggacagcagt ttgtttatca
      601 caaacggatc gggcgtggta tgcgtggccg gatatacagt atcattcact ttataacaaa
      661 tggttgacaa caatcccgat ttatagccca gctggcgaaa cgcattatac agcagggtag
      721 ctatggtggt tttgccattg gttccggtaa ctcccaccag tttgagtttg gctgagggat
      781 gaccgaaaaa attatcgcag ataatgccca aggcataact gctgtcgggt actttcacat
      841 aggtaacttc agggtccctt tcggcgggca acacttcgca cactatggcg gttgctccgt
      901 gttttacagc atcggcaata aatttatggc catcggcatt tacgcctttt actgcaacaa
      961 aaacactgtt ggcggcacat gcccttgaac tatagctgat atgcgaaata ccaatgccgg
     1021 cttttccgca tatttcggta aaccgggtgt ccgttaatat tttttccagt gactgtaaca
     1081 ttgtttcttc agatagataa gattaattcc actgttttgt tttccaaatt attccgctgt
     1141 tctttcaccc agccggcgcc tttgaccacg acgtccaacc cggctttttc cagcaggtaa
     1201 acggcatctt tagctgtcat gcctttcaca tcgggaattt tactgttttt caccgacatg
     1261 tcgttcacat aaattctttt gtaatcatcc gaacgggtag tgcttaccca gtcggaagcg
     1321 ccgcctttga atatcagatt gacattcagg ccggtgtaca cgtcctccac atcctgacgg
     1381 taggcgaaat ccagcaatgg agccgacaag gtatcaggca ttttcaattc tcttgatttt
     1441 atatccactc tggtagcaaa cactttatcg gctatttcct tgaaaaccgg cgcggcaacc
     1501 tggcttccgt acacttcgcc gccctgcatt ttgtattcca caacgatgca agaatatttc
     1561 gggtcatcgg cagggaagta gccgcaaaat gaggcaatat accggtcaat ataccctctg
     1621 gtaacttcat cccagatcct ggcggtacct gtttttccgg ctactttgta aggtgctttt
     1681 ctgatattgc gggcggtgcc tctggttacc acgccttcga gcagtgacct gactttttgt
     1741 aaggtagcat cggaacaaat ctgattgatt aatacctccg gctcattctt ttttaccacc
     1801 acgccttttc ttcgtatttc cttaaccagc tggggcttca tatattttcc gttgttggcc
     1861 acggcattgt acagcccgag tacctggagg ggtgtgagtt ttatggaata tccgaacgca
     1921 gcagccgtga ggtcttcata ctttatcggc aaatagggtg atggttcatt atccagatca
     1981 attcctgagc ggtctttcaa gcccattttt ctgaggtatt cttcaaattt tttgattttt
     2041 tctttgctca ggaacgccat cctggttatt tgtgttatgc ccacattgga agatttttca
     2101 aaagccttgg ccatcgaaac atcaccataa ccatcttcgt ggctgtccac cacttctttc
     2161 ttgtagtcag gataacgtat tttgcccgta tgtactattt gattggtatc atatttccca
     2221 tcttccagca cagcaatgac agaagcgagt ttcatcgtag atcccggctc ataaagttct
     2281 tctacagcat aatttctcat ttctttataa ttaccttctt tgtctttggt aagatttgct
     2341 attgccttaa tgtatccggt ttttacttcc attagtacta cacaacccca gtcggcatgc
     2401 gtagtatcca gactttttcg taaagcactc tgggctacat cctgcaaatt aacatcgatg
     2461 gcggtataaa ggtcacagcc gctctggggt tccaccatag cctcctcact gatttgtttc
     2521 cagctattgc ttcccaccct tttcaggtaa acgactcctc tggtgccccg caactgctct
     2581 ttatagtgac cttcgagccc cacaaaataa gattgggtta atttctggat gttgaggatg
     2641 cggttggtgt cttcagctat tagagggaaa cgtttgattt cggccagctg cagcacattc
     2701 atcctttttc ggattgatgc cgacgaaaca gatttttcat aagtctcttt cagtatcttt
     2761 tcataataag atttattgta atcattaaat gtgctgtaaa agcaatctgc cagggtatcc
     2821 acatactggt cgaaacgctg cttgttgacc ttgccggctt tcaggttgat atccacattg
     2881 tattcggcca aacgttcata acctatggtc atggcggcac aattttttat gggtctgatt
     2941 ctcttttctt tttttgtaat caccaggccg ccatattttc cgcgtcgtaa caacgggaat
     3001 ttttttatct gctgaagttc ggcataggta gcttttctca ccagcaaaaa attgcggtta
     3061 tatttcttgc gggctttttt cagctcgttg acatacttat atttattatt gggagttttg
     3121 tcgaaaaaca tactcaggca gatacccaga gaatctacat gtttattaaa cacggtatcg
     3181 tggatagcgg tggaatcaag gtcgagatta acctggaaca acggtatgga agtggccatc
     3241 agttcgccgt tttcggcata gatgtttccg cgggtgggct caataatttc ttttcgggtc
     3301 gattcggtag tggcctgaga tttccagtaa tcgtcgttga taaattgcag cgaaaatgtt
     3361 tttaccataa tggctatagc cactatccac accaggaaat aaattccata ggcccgcatc
     3421 aaaatggatt ttttcttttc gtttttctgg tccattggtt cttattttat gctgttgtct
     3481 tcctggattt tcataggggg caccactgat tccttgattc cggttcccag gctatcgagc
     3541 cggaaggcaa tttcagactg ttggctggca tgtgtcaggt ccgactgcgt ggtaatatat
     3601 tcgtctttga gttctataag ttcgtcgcgc agctgtgttg tcttacggat atttttttca
     3661 taattatatg aattggcgat gtacagcacg gccagcagac acaggaaaag cccgaaaggc
     3721 atgagtttca gcatggcttc gcgggtgagt atctctccgt tgagaatact ccggaaaaca
     3781 gacccaaggt tttgtctttt gtccttgtgg tctttcttct ttccttgagt cggtttttgc
     3841 tcttcagcag gagccggact aaattcattt ttttccatta ctattttttt tcagcgattc
     3901 taagttttgc actgcgtgct cttttgtttt gcgaaatttc tttttcatca ggagttatcg
     3961 ctttgcggct gataagttca aagggggtgt cggtattgcc aaacaagtca ctctcgatta
     4021 tcccgtcgaa gtttccggct ttcataaagt tttttaccaa ccggtcctcc agcgaatggt
     4081 aggagataac taccagcctt cctgattttt tcagtaccac caggctttgc atcaaaaatt
     4141 ctttcagggc gtcgagttcg ctgttggttt caattcgcag tgcctgaaag acttttgcca
     4201 ggtatttgtc ttcgtgtctt ttttcaatga gtccggaaat cgccgatctc aggtcatcgc
     4261 aggtaattat cttttgttgc ttgcgaaaag ctgcaatttt attagccagc tggctgctgt
     4321 tggacaattc gccgtattgc ctgaaaatct ccataagctg tcccgtagaa tactcattga
     4381 gtatatcggc cgccgatagt ttctgatttt ggttcatacg catatccaaa ggcgccccaa
     4441 accggaacga aaatcccctg gcggccacat caaactgatg tgaagatacg cccaggtcgg
     4501 caatgatgcc atctaccgga ataagccggt gatactgtaa taaattcttc aaataacgaa
     4561 aattgtgatt gagcagggta agccggtcat cgtctatcct gttggcttcc acatcagcat
     4621 cctgatcaaa agcaatgagc cgtccgcctt gtaatctttt tagtatttct ttcgagtgtc
     4681 ccccgccgcc ataagtaaca tctacgtaag tcccccctgg tctgatgagc aatccgtcaa
     4741 ccgctgcctg cagcaacacc ggatcatggt acatcttcct gaggttttga tggatggttt
     4801 cccatggtca attcagcgag gtcggaaaaa tccccgggtg catcggtcag cagtttttca
     4861 taaatttccc tgttccatac ttcgatgcgg tcaacatagg caaatacgat aacatccttg
     4921 tcgattttgg catattcaaa taatcttttc gggatgagta ttctgttggt tgcatccatt
     4981 tccacttcgg tggccccgcg ataaaaatat cttaaaaagt ccctgttttt ctttatatat
     5041 aaattcagtt ggctgatatc atgaacaatt ttttcccagg tatctatcgg atatagtgtc
     5101 aggcagggtt caaaacccct gttcaacaca aaacgatatc ctgaactttc gggaagcttc
     5161 cttttcaggg cagcaggaaa catcagccgg cccttaccgt caattttaca atcgtattcc
     5221 cctaataatt tttccatgat ttcagtcacg aagaatttct gcgtaaagat ataaacattt
     5281 gcacgatact gcactatatt gcaccaaaaa aagttttcaa caatttttgt taagttaaaa
     5341 tattgattta gtgtgtttta tatagtgcac taatttttta acaaaaaaaa tttataattt
     5401 tttgactacc catcttaaag ttttcaacaa ttttttgcta cagtttgcac acatttaaat
     5461 atttttaatt aaaatcgaag aattacgata aaaaaaccgt aaaccagagg caaaaaaacc
     5521 ttattatact aaggattaca ccaatgggcg gcggtttttc gctataaaat ccgtttgttt
     5581 ttgtattttt gcctctcata aatacatcct gaaataccca tgctttacaa acacttaatg
     5641 atgcgataac ccggcaggag gccgacaccc gatgaaacga ctgttaaaaa tacttctgct
     5701 gcctttttct gccatttacg gaagcatcac ttttatccgg aacaagtttt acgattgggg
     5761 ctggttaccc tcgcaggcct ttgatctgcc gcttatcagc gtcgggaaca tctccacggg
     5821 aggcacagga aaatcaccgg ctgttgaata ccttatccgc cttttgcaaa agcaatccat
     5881 cagcatcgcc accctgagcc gcggttatgg ccggcagacc aggggataca tccttgccga
     5941 caccggcgct acagccgcca ctatcggcga tgaaccacat cagtttttcc ggaaatttaa
     6001 tgatgtggca gtggctgttt gcgaagaccg tgtcaaaggc atagcccatc tcctcagacg
     6061 ggttcctgct cccggagtta tcctgctcga cgacgctttt cagcacaggc gtgtaaaacc
     6121 cggactcagc atattgctca cccggtacaa taatatatat tctcatgact ttgtgctacc
     6181 ggcaggccgg ttgcgggagt tcaggtgcgg tgccgcaagg gtggatatta ttatcgtaac
     6241 caaatctcct gagcaacttc cggaaacagc aaaagacaaa atcattaaaa gtttgaggcc
     6301 attgcccaat caggtggttt tgttttcgca tattgagtac aacgactttc aacccttttt
     6361 taaagaccag tgtcaaaaat atttgtcttc gacagaagat tttgaattat tattattcac
     6421 aggcatcgcc gatgccaggc cgctcatcac gcggctggag gccttgtgca aaaaacttca
     6481 catcatgcac tttggcgacc atcatgtgta ttcacaaaaa gacatacgaa ccatttcggg
     6541 gaaatttcgt aatattgtga cagaaaaaaa agccatcgtt accactgaaa aagatttttc
     6601 gaggctcgct catcaagaca tatgtaacga actgtcggat tttcctgttt tttacatccc
     6661 tgtcgagatg ggtttttcag aaaatgataa aaagatattt gaaaataaaa ttgtagatta
     6721 tgttgcaaaa aatcaatgaa accgttgctt acattaaaag caaaacttcc ttcgaaccca
     6781 gtattggcat tattctgggc accggcctgg gcaacctggc caacgaaatc aaggtacact
     6841 cggtgatacc gtatgctgaa attcccaatt ttcccgtatc tactgtaacg ggacataaag
     6901 gccagcttgt gttcggcaca ctgaacaaca tcaacgtggt ggccatgcag ggtcgttttc
     6961 attattatga aggatatgcc atgaaagaag tgggctttcc tgtgcgcgtg ctgaaatacc
     7021 tcggaataaa aaccctggtg ctgtcaaacg ccagcggcgg tgtaaatcct gggtttgaag
     7081 taggcgatat catgataatt accgaccaca tcaacctgat gggaaccaat ccgctgattg
     7141 gtaaaaacga tgatgaaatc gggccgcgtt ttccggatat gcatgttgcc tataacaaag
     7201 acctgataaa taaagccctg aagattgctg aaaaaaacaa tatccattgc cagcaaggtg
     7261 tttatgccgc tgttaccggc ccatgttttg aaacccctgc tgaatacaaa tatgtaagga
     7321 cagtgggcgc cgattgcgtg ggcatgtcaa cagttcccga ggttatagta gcgcaccata
     7381 tgggcctgcc tgtgtttgcg gtttcgatta ttaccgacct gggcgtggaa ggcaaaatag
     7441 tggaagtgtc gcatgaagaa gtgatacagg ccgctaacgc tgcagagcca aagatgacga
     7501 caatcatcaa agaactgatt gcaacgatat aataattttg ttaaacggat tttttgagta
     7561 aaaaatccca ggtcagcact tcaaggccca aaattccaga caaaacctcc tccccaggag
     7621 ttctcttcct ttggaatttt gggcatttta ttatcattta acgtgaatct ggtcgattat
     7681 ttttagccgc cacttatctt tgctcacttc gcatttccgg gaggtaaaca attgtaaaat
     7741 gcctccggtg gctaccacag ccgccgatat caaagttaca tccgctctaa cgacagggcg
     7801 gtctccgttg attacattat tgaccacatc caaaccaaaa aacagtcctc cgaaagccat
     7861 taacgatg
//
LOCUS       DAOSFP010000072         7857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_7981_length_7857_cov_4.111125, whole genome shotgun sequence.
ACCESSION   DAOSFP010000072 DAOSFP010000000
VERSION     DAOSFP010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7981_length_7857_cov_4.111125"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1087
                     /locus_tag="PLI16_03325"
     CDS             176..1087
                     /locus_tag="PLI16_03325"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOH83617.1"
                     /translation="MIPLFPVFKNLEMSDRQAVEKITSGFPPYAEYQFTTMCCWEIHQ
                     PFQLSTINGNLLLKQTNCLTGEYFFSLIGKNNLTATVRQVLDFLSVNKLPTVLKCLPE
                     EMLEHLDQSQFNICEDRDNFDYIYPVAGFHEARGSSYKVFRHKCAAFKTNYPDIITRS
                     FDLTLPQNSSQILTLYDTWASAKAAGNKDFESAGEHYALKKLLSMARNLDSVGAIGLF
                     DRQTMIAFSIFEINDTKFITGLFCKANTNYKGVYQYLMKEVVALSHNRGIEYLNWEAD
                     LGLESLRKSKMELHPAFFLKKYILEPV"
     gene            1142..1777
                     /locus_tag="PLI16_03330"
     CDS             1142..1777
                     /locus_tag="PLI16_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83618.1"
                     /translation="MKHWFFLACFLPALPVMVNAQEQDSLFHDAGIYTTVQWSAGEPA
                     IVTPQFWVYIGDYYLETRYNYENSETASLYFGRSFSFGENTSVEITPILGGVVGATNG
                     ISPGFNFNLEYKSFATSTQCQYTFNLKDFPGSFFWDWTNFSVNFSEHFGMGGAIQLYI
                     PQSGEKNISVGPMIVLGYKKFSLEMYVYNFWECQPTYAVGLVYDFMPSSDD"
     gene            complement(1836..4769)
                     /locus_tag="PLI16_03335"
     CDS             complement(1836..4769)
                     /locus_tag="PLI16_03335"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOH83619.1"
                     /translation="MKKSSLIILLFLVLVTAVCAQQKDYTEYVNPFIGTGGHGHTYPG
                     ACAPYGMVQLSPDTRLTGWDGCSAYHYSDQVIYGFSHTHLSGTGCSDYGDILLMPVSG
                     DVNLKDYAYASSFSHKNEKAKPGYYAVKLDKHNIQVELTATQRAGMHRYVFAGTTQNS
                     IILDLEHRDQVIGSYVEFVNNTTVRGMRTSRAWAQKQILYFEIQFSKPFSHYGLCEID
                     SSGNIKKTSTGKASGKQVKAFFTFEGQKEILVKIGLSATSAEGAAKNLAAEMSSWDFT
                     SVYLQTKKEWNKELGKIEVEGGTKKMTTTFYSALYHCMITPNIYCDVDGKYLGRDLKV
                     HDSKGSDYYTVFSLWDTYRAEHPLLTIIDQKRTNDFINTFISQYLEGGRLPVWELSAN
                     ETFCMIGYHAVPVIYDAYTKGIKNFNTAKAYEAMKTSAMSDDFGLKFLKQYGYVPGDK
                     EHESVSKTLEYAYDDWCIAQMAKALGKTDDYKKFIKRAQFYKNIFDTQTGFMRPKMNG
                     AWQTPFDPAEVTFQYTEANAWQYSFYVPQDLSGLIQLYGGKANLANKLDELFNTKQQL
                     TGREQSDITGLVGQYAHGNEPSHHMAYLYNYLNQPWKTQQLCHKIMTTLYHDKPDGLC
                     GNEDCGQMSAWYVLSALGLYQVCPGNTQFAIGTPLFAEAVIHLENGNTFTIKAKKASL
                     KKFYIQSAKLNEQPYTRSFLTYEDIMNGGKLEFVMGAKPNKRWGSADEDIPVTAINDH
                     LLLPTPYVTHTSKTFTDSIEIELKNILSGTGIYYTTDGTQPDKTKTKYEGPFTIKETA
                     KIKAVSFNPAYDASYVMVSDFIKINLNRKIKLLSKYSSQYTGGGDNALIDGIRGGENF
                     RLGEWQGYDSVDFEAIVDLGNKQKINTISTGFLQDIGSWIWMPVYVEYSVSDDGVTFK
                     PVAEIKNTVPINDYKCTIHDLTSDVKTEGRYVKVKAYNIGTIPEWHLGAGYSGWIFVD
                     EIMID"
     gene            complement(4782..7325)
                     /locus_tag="PLI16_03340"
     CDS             complement(4782..7325)
                     /locus_tag="PLI16_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HOH83620.1"
                     /translation="MLKKQYRHLLLFIAFCSTNIIAQNTNKVFLINDWQFSESGAKMC
                     HAARVPGTIHTDLLENNLIKDPYYACNEKDLQWIEAKDWEYKTVFPADEKLLKNEHIV
                     LVFEGLDTYARVYLNNTLVITADNMFRRWTTEVKPLLKKDSNELKVVFESTVNKAKEQ
                     AAGLRYTLPGDEKVFVRKAQYQFGWDWGPRYVTCGIWRPVYLEFWNNAKITNTQVVQK
                     SVGKESAELSFVNTILAEKNCETEIQIEDTKTDVLYVSQKINLKKGSNKVAVNIKINR
                     PELWWTNGLGKPFLYSFKISLLEGKTTLDESTVRTGIRTLELVSEKDDIGESFYFKLN
                     GVPVFMKGANYIPQDNFVPRVKEEEYRDLILQAKESRFNMLRVWGGGIYENDIFYDLC
                     DEHGILVWQDFMFACAMYPGDSLFTANVREEVLQNVVRLRNHPCLALWCGNNEIEEGW
                     NNWGWQKQYKYSDADSLEIWNNYLKLFRKDIPKIIQLADSGRFYRPSSPKYGWGRDQS
                     LTDGDSHYWGIWWGMQPFETYTAKVGRFASEYGFQAFPEKSTFQKVALPDEWVLDSEA
                     LKCHQKHPTGYETIKTYMQREYKVPCNIDDYIYVSQLLQAYGIKTAIEAHRNAKPRCM
                     GSLYWQFNDCWPVVSWSGMDYYHNKKALQFFVKKAFNDIVVTLTNNEKNITVQLISDK
                     TEFVPSQVELKLMDFHGKVRWEEKRFVNIKPGMNKVIEKIDKRLLLADVSLHKSLVLS
                     ARVIVDDRVVSQNYLYFKKPKDLALEDPEISYEVSKLPEGLEIKISTKLLAKNVFISF
                     ENCDSRDIQLSDNFFDMLPGSTVVILAKTMENPDEIIKNLRVRTLFDVH"
     gene            complement(7331..>7857)
                     /locus_tag="PLI16_03345"
     CDS             complement(7331..>7857)
                     /locus_tag="PLI16_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HOH83621.1"
                     /translation="QSYVIRTIEKFVNSKGKSIIGWDEILEGGLAPNAAVMSWRGADG
                     GIAAAQLKHYVVMCPGEFCYLDHYQGDPATEPLAFGGYTPLEKVYSYNPMPDSLPENL
                     KKYILGAQGNLWTEYITDFSHLQYMALPRMIALSEVLWTNPKIRDYERFYLRLQKHTE
                     TLDLLKVNYRKPTK"
BASE COUNT         2033 a         1761 c         1649 g         2414 t
ORIGIN      
        1 catggtaata aagtgtacac aagaattgca taaaatgaca aaagtattat ttaccgctta
       61 gctgttaaag tgtttaaaac atataaaatt ataccaggat ttttgcttat ttttgggttg
      121 acatctgctg gctaacgaca aaaataatgg attacatttt atcaaactat caattatgat
      181 ccctttgttc ccggtgttta agaatctgga gatgtcagac aggcaagctg ttgaaaaaat
      241 cacttccggg tttccacctt atgccgaata ccaattcacc accatgtgct gctgggaaat
      301 tcaccagcca tttcaactct caacaataaa tggcaacctg cttcttaagc aaaccaactg
      361 cctgacaggc gagtattttt tttcactgat cggcaaaaac aatctgacag ccaccgtgag
      421 gcaggtgctg gattttttgt ctgttaataa gcttcccaca gttttaaaat gcctgccgga
      481 agaaatgctg gagcaccttg accaatcgca gttcaatatc tgcgaagatc gcgataattt
      541 tgattacatt tatccggtgg ccggcttcca tgaagcccgc ggaagcagct ataaagtttt
      601 ccgccacaaa tgcgctgcct ttaaaaccaa ttatcctgac atcattacgc gttcttttga
      661 tcttacttta cctcaaaaca gtagtcagat attaactctt tacgatacct gggcaagcgc
      721 taaggctgcc ggcaacaagg attttgaatc cgccggcgaa cattacgcat taaaaaaact
      781 attgtcgatg gcccgtaatc tcgattccgt tggtgccatt ggactttttg accgacaaac
      841 catgattgca ttttctatct ttgaaatcaa tgatacaaaa tttattaccg ggcttttctg
      901 caaggctaac actaactaca agggcgttta ccagtatctg atgaaagaag tagtcgcctt
      961 atcgcacaac cgcggaattg aatatcttaa ctgggaggcc gatctggggc tggaatcctt
     1021 acgtaaatca aaaatggaat tgcatccggc atttttttta aagaaataca tcctggagcc
     1081 ggtataatca tttgagcctt tctgccagct tctatcaaat aaaaaatcat atttttacat
     1141 tatgaaacac tggttttttt tggcttgttt tctgcctgcg ttacccgtaa tggtgaatgc
     1201 acaggaacag gacagcctgt ttcatgatgc cggcatttac accacggttc aatggtcggc
     1261 cggagagcct gcaatagtta caccccagtt ttgggtttat attggcgatt actaccttga
     1321 aacacgatac aattatgaaa actctgaaac ggcatccctg tattttggcc ggtcgttttc
     1381 ttttggggaa aacacttcgg tagaaataac gccaatactg ggaggcgttg tcggcgccac
     1441 caacggcata tctccggggt ttaatttcaa tctggaatat aaatcatttg ccacttcgac
     1501 acagtgccag tacaccttta acctgaaaga ttttcccggc agttttttct gggactggac
     1561 aaatttcagt gtcaactttt ctgaacactt tgggatgggc ggcgccatac agttgtatat
     1621 tcctcagtcc ggagaaaaaa acatttcggt tgggcctatg atagtgcttg gttacaagaa
     1681 gttttcactg gaaatgtatg tttataattt ctgggaatgc cagcctacct atgccgtggg
     1741 attggtttat gattttatgc cgtcatcgga tgactaaaaa caataaaaca ttgcaataat
     1801 tttgatttgg gccgtcatac gatgatcgct ttaaattaat caatcataat ctcatcaaca
     1861 aatatccatc cgctataccc cgcacccagg tgccattcgg gaatggtgcc aatattatat
     1921 gctttcactt ttacgtagcg gccttcggtt tttacatcag atgtcagatc atgaatcgta
     1981 cacttgtaat cattaatggg aaccgtgttt ttaatttccg ctacgggctt aaaagtaacc
     2041 ccatcgtcgg aaacgctgta ttccacgtaa acaggcatcc atatccagga accaatgtcc
     2101 tgcaaaaagc ccgtcgaaat cgtattgatt ttttgtttgt ttccgagatc aacaatagct
     2161 tcaaagtcaa cgctgtcgta tccctgccac tcgcctaacc tgaagttttc gcctccgcgt
     2221 atcccgtcaa tgagtgcatt atcgcctccg ccggtatatt gcgaactgta tttggataaa
     2281 agttttatct ttctgttgag attaattttt ataaaatccg ataccatcac atagctggcg
     2341 tcataggcag gattgaacga taccgctttg atttttgccg tttccttaat cgtaaatggt
     2401 ccctcgtatt ttgttttcgt tttatcgggt tgggttccgt cagttgtata atatatcccg
     2461 gtgccgctga gaatattttt taattctatc tcgatgctgt cggtaaaagt ttttgatgta
     2521 tgagttacat aaggtgtagg gagcagcaga tggtcgttta tggcagtaac aggaatgtct
     2581 tcatcggcag agccccagcg tttatttggt ttcgcaccca tgacaaattc gagctttccg
     2641 ccattcatta tgtcttcata ggtgagaaaa cttctggtat agggctgttc gttgagtttt
     2701 gccgattgaa tgtagaattt tttcagggaa gctttttttg cttttatggt aaacgtattg
     2761 ccgttttcga gatgaattac ggcttcggca aacaggggag tgccgatggc aaattgcgta
     2821 tttccgggac aaacctgata taaccccagg gcgctcagca cataccacgc cgacatctgc
     2881 ccgcagtctt cgttgccgca taaaccgtcg ggtttgtcgt ggtataaagt agtcataatt
     2941 ttatgacaca actgctgtgt tttccatggc tgattaaggt aattatagag gtacgccatg
     3001 tgatggctgg gttcgttgcc gtgtgcatac tgccccacaa gtcctgtaat atccgattgt
     3061 tcacgtccgg taagttgttg ttttgtgttg aacaattcat cgagtttgtt tgccaggttg
     3121 gcttttccgc catacaactg tataagtccg ctaaggtcct gaggtacata aaaactatat
     3181 tgccaggcgt tagcttcggt atattgaaaa gtaacctctg cggggtcaaa gggtgtctgc
     3241 caggcgccgt tcatctttgg ccgcataaag ccggtttgtg tgtcgaagat gtttttataa
     3301 aattgtgccc gtttgataaa ctttttataa tcatcggttt ttcccaaagc tttggccatt
     3361 tgcgcaatgc accagtcgtc gtatgcatac tccagcgttt tggaaaccga ttcgtgttct
     3421 ttgtcgccgg gaacatagcc atactgtttg aggaacttca ggccaaaatc gtcactcatg
     3481 gcgcttgttt tcatggcctc ataagctttt gcggtgttaa agttttttat tcctttggtg
     3541 taagcatcat aaatgacggg aacagcatgg tatccgatca tgcaaaaagt ttcgttagcc
     3601 gaaagctccc acacgggcag ccggcctcct tcaaggtact gactgataaa tgtattaata
     3661 aaatcattgg ttcgcttttg gtcgataatg gtcagcagag ggtgttccgc gcgataggta
     3721 tcccaaaggg aaaacaccgt gtaataatca gaacctttgc tatcgtggac ttttaaatcc
     3781 ctgccaagat attttccatc cacatcgcaa tatatgttgg gtgtaatcat gcagtgataa
     3841 agagccgagt aaaaagtggt ggtcattttt tttgtgccgc cctctacttc aatttttccc
     3901 agttctttgt tccattcttt ttttgtttga aggtaaactg aagtaaagtc ccagctactc
     3961 atttcggcgg caagattttt ggccgctcct tcagccgaag tggcgcttag gccgattttt
     4021 accagtattt ctttttgtcc ctcgaaagta aaaaacgctt tcacctgttt gcccgaggct
     4081 ttgccggttg atgttttttt tatgttgccg ctggaatcaa tctcgcacag accataatga
     4141 ctaaatggtt ttgaaaattg tatttcgaaa tacagaatct gtttttgtgc ccaggcccga
     4201 gaggttcgca tgccacgcac ggtggtgttg ttgacaaatt ccacatagga gccgatcacc
     4261 tgatcgcggt gttcaaggtc aagaatgatg ctgttttgtg ttgttccggc aaacacatac
     4321 ctgtgcatac cggcgcgttg agtggccgta agttcgacct gaatgttgtg cttatcgagt
     4381 tttacagcat aataaccggg tttcgctttt tcatttttgt ggctgaaaga ggaggcgtat
     4441 gcataatctt tcagatttac gtcgccactg acaggcatca gcaagatatc gccataatcg
     4501 gagcagcccg tgccactcag gtgggtgtgc gaaaaaccat agataacctg gtcggagtaa
     4561 tgatatgccg aacagccgtc ccagccggta agacgggtgt cgggactgag ctgcaccatg
     4621 ccatatgggg cgcaggcgcc gggatacgta tgcccgtgtc cgcccgtgcc aataaaagga
     4681 ttgacatact cagtgtaatc tttttgctgg gcgcaaactg cagtaaccag caccaaaaaa
     4741 agaagaatta tcaatgaaga ttttttcatg ccggaaagtt tttaatgtac atcaaacaag
     4801 gttcgtaccc gcagattctt aattatttca tcagggtttt ccatcgtttt tgctaaaatt
     4861 accacagtac ttcccggtag catgtcgaaa aaattatcag atagctggat gtcgcggcta
     4921 tcgcagtttt caaaactgat aaacacgttt tttgccagca gctttgtgct gattttaatt
     4981 tccagaccct ccggcaactt actaacttca taactgattt cagggtcttc aagtgccaga
     5041 tccttaggct ttttaaagta taaatagttt tgagaaacaa cccggtcatc aactatcact
     5101 cttgccgaga gtaccaggga cttgtgtaac gaaacatcag caagaagaag tcttttgtct
     5161 attttctcta ttaccttgtt cattcccggc tttatgttta caaatcgttt ttcttcccac
     5221 cgcactttgc cgtgaaaatc catcaacttc aattccacct gcgagggtac aaattccgtt
     5281 ttgtcggaga ttagctgtac cgtgatattt ttttcattat tggtcagcgt tacaacgata
     5341 tcattaaatg cttttttcac gaaaaactgc agagcttttt tgttgtgata ataatccatg
     5401 cccgaccagg aaaccacggg ccagcaatcg ttgaattgcc agtagagcga ccccatacag
     5461 cgcggcttgg cattacgatg ggcctcaata gcggttttaa ttccgtatgc ctgcaacaac
     5521 tggctaacat aaatataatc gtcaatattg cagggaacct tgtattcgcg ctgcatatat
     5581 gtttttattg tctcatatcc tgtcgggtgt ttctgatgac atttgagggc ttccgaatcc
     5641 aatacccact catccggtaa ggcaactttc tgaaaagtac ttttttcggg gaatgcctga
     5701 aatccgtatt cactggcaaa acgcccgact tttgcagtat aagtttcaaa tggctgcatg
     5761 ccccaccata tgccccagta atgtgaatcg ccatcagtaa ggctttggtc tctgccccag
     5821 ccgtatttcg gcgatgatgg ccggtaaaac cttcccgaat cggccaattg gattatttta
     5881 ggtatgtcct tcctgaaaag tttcaggtaa ttattccata tttccaggct atcagcatcg
     5941 ctgtatttgt actgtttctg ccatccccag ttattccagc cctcctctat ttcgttgttg
     6001 ccgcaccaca aggccaggca gggatgattt cgcaagcgca caacgttttg caaaacttct
     6061 tcgcgaacat tggctgtaaa taaactgtcg ccagggtaca tggcacaggc gaacataaaa
     6121 tcctgccaca caaggatccc gtgttcatca cacaaatcat aaaaaatatc gttttcgtaa
     6181 atccctccgc cccacacacg caacatgttg aacctcgact ctttggcttg caaaattaaa
     6241 tcccggtatt cttcctcttt tacgcgcggt acaaagttgt cctgcgggat ataatttgcc
     6301 cctttcataa atacgggcac tccgttcagc ttaaaataaa acgattcgcc aatgtcatct
     6361 ttttcagaaa ccagctccag agttcggatt ccggtacgga cagtagattc gtcaagggtt
     6421 gttttcccct ccagaaggct tattttaaaa gaatataaaa aaggcttgcc caaaccattg
     6481 gtccaccata attccgggcg gtttatttta atgttgacgg caactttgtt gctccctttt
     6541 ttcagattga ttttttgtga aacatagagc acgtcggtct ttgtatcttc aatttgtatc
     6601 tccgtttcgc agtttttttc agccaggata gtgttgacaa aagatagttc cgcagattct
     6661 ttgccgaccg atttctgaac aacctgcgta ttggtaattt tggcattatt ccaaaattca
     6721 aggtaaacag gcctccatat tccacacgta acataacggg gaccccagtc ccagccaaac
     6781 tgatattgcg ccttacgtac aaaaactttt tcgtcgccgg gcagggtata gcgcaggccg
     6841 gcggcttgtt ccttagcttt atttaccgtc gactcaaaaa caaccttaag ttcgttgctg
     6901 tcctttttca ataaaggttt gacttcggta gtccaccggc gaaacatatt gtcagcagtg
     6961 atcaccaggg tattgtttag gtaaactctt gcataagtat ccaggccttc aaaaaccaac
     7021 acgatgtgct catttttcag taatttttca tccgccggaa aaaccgtttt atactcccag
     7081 tctttagctt ctatccactg caggtctttt tcgttgcagg catagtaagg atcttttata
     7141 agattgtttt cgagaagatc ggtgtgaatg gtgcctggaa ccctggcggc atgacacatt
     7201 tttgctccgc tttctgagaa ttgccagtca tttatgagga aaaccttatt cgtgttttga
     7261 gcaataatgt tggtggaaca aaaagcgata aacaacaaca aatgtcggta ctgttttttc
     7321 agcatatttt ttattttgta ggcttcctgt aattcacttt cagcaaatcc agtgtttcgg
     7381 tgtgtttttg cagccttaaa taaaaccgct cgtaatcgcg gattttcggg ttggtccaca
     7441 acacttcact cagggcaatc atgcgtggaa gagccatgta ttgcaggtga ctgaaatcgg
     7501 taatatattc tgtccacaaa tttccctgtg ctcccagaat atattttttc agattttccg
     7561 ggaggctgtc gggcatggga ttgtatgaat aaactttttc caggggcgta tagccgccaa
     7621 aggccagtgg ctctgtggct gggtcgccct gataatgatc caaataacaa aattccccgg
     7681 gacacatcac aacataatgt ttcagttggg ccgcagcaat accgccatcg gctccgcgcc
     7741 acgacatcac agcggcattg ggcgccagtc cgccttcgag aatctcgtcc cagccgataa
     7801 tactttttcc cttgctgttt acaaattttt ctattgtcct gattacataa ctctgaa
//
LOCUS       DAOSFP010000073         7832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8029_length_7832_cov_4.503022, whole genome shotgun sequence.
ACCESSION   DAOSFP010000073 DAOSFP010000000
VERSION     DAOSFP010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8029_length_7832_cov_4.503022"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLI16_03350"
     CDS             complement(<1..1065)
                     /locus_tag="PLI16_03350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH83622.1"
                     /translation="MKRFIILVLSVFFCGMVNAQVNDEVLLTVAGEKITKTEFLNVYK
                     KNNIKGDLMDKKSLDEYLNLYINFKLKVKESEELKLDTFTSFKNELAGYRKQLAQPYL
                     TDKQVDDDLLKEAYDRLQWDIRASHILIKVNKDATPEDTLAAYKKVLKIRERLLKGED
                     FATVAKETSDDPSARDREEPDSKSIFKGNGGDLGYFSVLDMIYPFENGAYNTKVGQVS
                     LPVRTDYGYHLIKVVNRKPAMGRAVVAHIFIEVPKDAKPENLATYKSKIDEAYAKLKG
                     GETFENVVKEYSDDKGSAAKGGVLQGFGVNRMVPEFIEAVAKLNLNEYSEPVQTVYGW
                     HIIKLIERTPVKSFDEEKTQL"
     gene            complement(1131..2594)
                     /gene="guaB"
                     /locus_tag="PLI16_03355"
     CDS             complement(1131..2594)
                     /gene="guaB"
                     /locus_tag="PLI16_03355"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOH83623.1"
                     /translation="MESEKFLGEGLTYDDVLLIPAYSDVLPRMVDVSTLFTRNIKLKI
                     PIVSAAMDTVTESHMAIAIAQEGGMGVLHKNMPIADQANEVKKVKRAENGMIIDPITL
                     KKDAYVKDALDLMREYKIGGIPVIDKKNRLVGIITNRDLRFERNHNRPISEVMTKENL
                     ITTTEFTDFEKAADILQEHKIEKLPVVDKDFKLIGLITYKDIIKIKARPNACKDERGR
                     LRVAAGVGVTKDMLDRMSELVKAGVDAIVIDTAHGHSKGVIDAARLFKKNFPEIDMVV
                     GNIATAEAAKDLIKAGADAVKVGIGPGSICTTRVIAGVGVPQLYAIYSIAKAIKGSGV
                     PLIADGGIRFSGDIVKAIAAGAHSVMLGSMLAGVDESPGETVIFEGRKFKTYRGMGSI
                     EAMQRGSKDRYFQESEDDALKLVPEGIVGRVPYKGTLSEVIHQMVGGLKAGMGYCGAK
                     NIEKLQKAKFIKITPASVAESHPHDVIITREAPNYSR"
     gene            complement(2825..6223)
                     /locus_tag="PLI16_03360"
     CDS             complement(2825..6223)
                     /locus_tag="PLI16_03360"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH83624.1"
                     /translation="MIKPVKTFLLLILLLGSQLFTDGNLLASHYMGGEITWECLSNGR
                     FRFIMKLYRECNGITYSTGEIMHIQGYPGLTQITMALKPGANPHDIDDGILDGKTDIS
                     PDCWNYDSEIKCLPTPSTANTGAIEEWYFTSDVAYPAGVLLSGVPPTSGWIFYNDGCC
                     RNPSANMVNPTSEDWFLRAIMYPYNGQNTNPCYDNSPVFAEVPSTVICTGYPFKYNHN
                     ATDKELDSLAFSWAPALQNATTNMGYMGTYTYNSPLPGTAQNPLNVPATMNPYNGEIS
                     YTSYTSGAFVTVTKVTAYRCGVKIAEIFREMQIVLLACPGSNTPPSVAGPFYNANLGI
                     YEFSDTVYAGDTVDVDITGIDYGVLPNNNPQTVTLEASGQDFGAGYVSETSGCPRPPC
                     ATLTPPPTLSAMMYVSTHFHWRTTCDHLTHPASITGIPGICGTMFNVHNFVIKVYDDF
                     CPAPGIKIATISIVVLPTPVLPPPEPKCTRVHPNGDITLNWIPPIDTMNSFNGFFVYH
                     ASTPAGPFVKIDSIFDINQTTKTYSGLGGNAGQQYFYMTTRSGCYGLYQSAPSDTVST
                     IYLNVTAIGGGPIAQLNWNPVHNPLFTSSSQYYHIYREFPAGSWNFLDSTNQLTYLDT
                     VTICSAFINYRIEIADSSGCVSVSSVDGEQLHDGFPPDPTMLDSVSVDFATSKAILGW
                     QPNTSPDAVKYYIYRKDIGTGAWFIRDSVDVPATSYMDMTSTPQNNSETYCIAAVDSC
                     KKLSPMSPEHKTIYLSPPIINACADRITLQWTSYINFVNPGLQGYRLLVSENGGPYTL
                     LADLPANGLSYEHTGLVNGSNYCYLVRAYDSLSQKTSTSNSQCVVVTKPNQPKFIYLR
                     YATVQNNDFIRLGFYVDSTAYITKFKILRSVDGINYDTIDQLPPNNPYSNVTYDDNSV
                     NVNEKSYYYKVVVSDSCSLDMLTSNVGRSIFLEGTVPEYLLNNLYWNHYEDRVPVAYN
                     LWREVDQYEPYTKIISLVMNESAYTDNVENYTESGGRFKYMIEAELYDVYDSTYVFED
                     KVLSNEIVLLQPPRLYVPNAFAPYGLNNLFKPVGVYTEKDGYQMIIYDRWGRKIFETT
                     DYETGWDGTFEGKICALGVYTYYVKITNAFNKTFIKRGIVTLVR"
     gene            complement(6367..7653)
                     /locus_tag="PLI16_03365"
     CDS             complement(6367..7653)
                     /locus_tag="PLI16_03365"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH83625.1"
                     /translation="MINRKQPPALKVAHKLHIPEPRNEKLVNNIPVYLFDLGETAITK
                     IDFIFEAGNLHQPAPLLAFYTNKMLTEGTLSYSSFEIAQKLDYYGAWLNKTVSKDHAV
                     VSLSVLNKHLKYVLPVLNEIINHPKFSDDELKTHTRKDKQAFIDDGRKVNEVAGLHFN
                     HQLFGKHHPYGSLTTAADFDRQQAAKLQAFHDQRYTAEKCNVVVSGKLSKDILPLLNY
                     FFGYSKKGKQKSENRASSVICPGKQKQFIEMPSAVQSAIRIGKISINKTHEDFMKLYI
                     LNTILGGYFGSRLMKNIREDKGYTYGIYSMMMSFQQAGVFLIASEVGAEVCQRAVDEI
                     YKELKILNTELIVQDELELVKRYMAGSVLRSFDGPYQVADRFKSLLKYGINYKKYYMD
                     FMNTINAVTAEDLLHTSQKYLQESSMLELIVGKAGK"
     gene            complement(7650..>7832)
                     /locus_tag="PLI16_03370"
     CDS             complement(7650..>7832)
                     /locus_tag="PLI16_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOH83626.1"
                     /translation="RALRLAVSELLGDVDLVNSEAQNYSRVTAADIQEAANKVLVDSN
                     CSVMYYMAAAKRKKNR"
BASE COUNT         2078 a         1737 c         1716 g         2301 t
ORIGIN      
        1 aagctgggtt ttttcttcgt cgaaagattt caccggggtt ctttcgatga gttttatgat
       61 gtgccatcca taaaccgttt gcacaggttc tgagtattca ttaagattga gtttggccac
      121 ggcttcgata aattcaggca ccatacggtt tactccaaaa ccctgcagca caccgccttt
      181 tgcagcagag cccttatcat ctgagtattc ttttaccacg ttttcaaaag tttctccgcc
      241 ttttaatttc gcataagcct cgtcaatttt agatttgtag gtagccagat tttcgggttt
      301 agcatcttta ggcacttcaa taaagatatg agccaccaca gccctgccca tggccggttt
      361 tctgttcact actttaatca ggtgatagcc ataatcggtg cgtaccggca gtgaaacctg
      421 acccactttg gtgttgtagg ctccgttttc aaaaggataa atcatatcca gcacggagaa
      481 ataaccgagg tcgccgccat tgcctttaaa aattgatttt gagtcgggtt cttcgcggtc
      541 acgtgccgaa gggtcgtcgg aagtttcttt ggctacggtg gcaaagtcct cgcctttgag
      601 caggcgttcg cgtattttca gcactttttt ataggctgcc agggtatctt caggagtggc
      661 atctttattg acctttatca ggatatggct ggctctgata tcccactgca gcctgtcata
      721 ggcttctttt aaaaggtcat catcaacctg tttgtcggta aggtaaggct gagccagttg
      781 cttgcggtat ccggcaagtt cgttcttaaa tgaagtaaac gtgtcaagtt tcagctcttc
      841 agattctttt actttgagtt taaaattgat atacaggttc aggtattcat caagtgattt
      901 tttgtccatt aaatcgcctt tgatattgtt tttcttatac acattcagga attctgtttt
      961 tgtaatcttt tcgccggcta cggtaagaag tacttcgtca ttaacctgtg cgtttaccat
     1021 tccgcagaaa aatacggaca atactaaaat aataaacctt ttcatagttt tcgatctgat
     1081 tttataaaat aaagtgcaaa aatatgtttt cttggaacaa attcaaaaaa ttatctgcta
     1141 taatttgggg cttcgcgggt aatgatcaca tcatgcgggt ggctttcggc cacactggca
     1201 ggtgttattt ttatgaattt ggctttttgt agtttttcga tattcttagc gccacaatac
     1261 cccattcctg ctttcaggcc tcccaccatc tgatggatca cctccgacaa ggttcctttg
     1321 tagggcaccc gtcctacaat gccttcgggc acgagtttca gggcatcgtc ttcactttcc
     1381 tgaaaataac ggtctttaga accgcgttgc atggcctcga tagaccccat tccgcgatag
     1441 gttttgaact tgcggccttc aaaaatcacg gtttcgcccg gcgactcatc cactccggcc
     1501 agcatagaac ccaacatcac tgaatgtgcg cctgccgcaa tagcttttac aatgtcgccg
     1561 ctgaaacgta tgccgccgtc ggctattaaa ggcacgccag agcctttgat ggctttggct
     1621 atagaatata tggcatacag ttgaggaaca cctacaccgg caattacccg agtggtgcag
     1681 atggaccccg gaccgatacc cactttaacg gcatcggcgc ctgcttttat caggtctttg
     1741 gcggcttcgg ccgtggctat attgcccacc accatatcaa tttccggaaa atttttctta
     1801 aatagtctgg cagcatctat tacccccttg gagtgtccgt gggcagtgtc aataacaata
     1861 gcatccaccc cggctttcac caattcactc atgcggtcaa gcatatcttt ggtaacgccg
     1921 acccctgcag ctacccggag ccttccccgc tcatccttgc aggcattggg gcgtgctttg
     1981 atcttaatga tatccttata ggtgatcagg ccaatcaatt tgaaatcctt atccacaaca
     2041 ggcaacttct ctattttatg ttcctgcaat atatcggcag ctttttcaaa atcagtaaat
     2101 tcggtggtgg ttatcaggtt ttctttggtc attacttccg atatcggccg gttgtgattt
     2161 cgctcaaacc gcaaatcgcg gttggtaatt atacctacca ggcggttttt tttatcaata
     2221 accggtatgc cgccgatttt atattccctc atgagatcga gggcatcttt aacataagca
     2281 tcctttttga gggttatcgg atcgattatc atcccgtttt cggcacgttt tacctttttc
     2341 acctcatttg cctggtccgc aataggcatg tttttgtgaa gtacgcccat gcctccctcc
     2401 tgggcaatgg caatagccat gtgcgactca gtaacagtat ccatggcagc agacactatg
     2461 ggtattttta attttatgtt tctggtgaac agcgtggaaa catccaccat acggggcagt
     2521 acatccgaat aggccggtat taataataca tcatcatatg ttaatccttc gcctaaaaat
     2581 ttttccgatt ccataaatta tacattgttt tgagttttgc aaaaatatga aaaatagttg
     2641 gttattacca ttttaacaaa atagtctttt aaaaaagggg ctgtctcaaa aggtttactt
     2701 tgtgattttc accgccttct ttcccctccc taaagggtgg aaatcgctga aaatcagcac
     2761 tccctttagg gatggggtaa tgatgatttt cagcaaaagc tagttttgat acagcctctt
     2821 ttttttacct caccagggtg acgattcccc ttttaatgaa agttttatta aaagcatttg
     2881 ttatcttcac gtaataggtg taaacaccca gtgcacaaat ctttccttcg aaagtgccat
     2941 cccatcctgt ttcatagtca gtcgtttcaa agattttgcg accccagcga tcataaataa
     3001 tcatctggta tccgtccttt tcggtatata ccccaaccgg tttaaagaga ttattgagtc
     3061 cataaggtgc aaatgcattg gggacatata acctgggagg ctgcaacaaa actatttcat
     3121 tcgaaagcac cttatcttca aatacataag tactatcata aacatcatac agttccgctt
     3181 cgatcatgta tttaaagcgg ccgccgcttt cggtatagtt ttctacgttg tcggtatatg
     3241 ccgattcgtt cattaccagg gaaataattt tggtataagg ctcatactga tctacttcgc
     3301 gccataagtt ataggccacc ggtactctgt cttcataatg gttccaatac aagttgttca
     3361 acaggtactc cggaactgtc ccttcaagaa aaatggaacg tcccacatta gaggtgagca
     3421 tatccagcga acagctatcc gaaacgacca ctttgtaata ataactcttt tcgttcacat
     3481 tcaccgagtt gtcgtcgtag gtcacgttag aatatggatt gttgggaggg agctggtcga
     3541 tggtatcata attaatgcca tctactgacc ggagaatttt gaattttgta atgtatgcag
     3601 tggaatccac ataaaatccc aggcgtataa aatcgttatt ctgcacggtg gcatatctga
     3661 gataaataaa cttcggctgg ttgggctttg taaccacgac acattgtgaa ttggaggtgg
     3721 aggttttctg gctaagagag tcatacgctc taaccaggta acagtaattg gagccgttaa
     3781 ccaatccggt atgctcataa ctcaacccat ttgccgggag gtcagccagc aacgtatagg
     3841 gtccgccatt ttcactcact aaaagccggt agccttgtaa tccggggtta acaaaattga
     3901 tgtatgaagt ccactgaagc gtgattctat ctgcacaggc attgattatt gggggtgaca
     3961 ggtagatagt tttgtgctcc ggactcatgg gacttagctt tttgcaacta tctacagcag
     4021 caatgcaata agtttcagag ttgttctgcg gagtagaggt catatccata taagaagtag
     4081 ccggcacatc cacggaatcc ctgataaacc aggcaccagt accgatatct ttgcggtaga
     4141 tatagtattt taccgcatcg ggtgatgtat ttggttgcca gcccagaatc gcttttgagg
     4201 ttgcaaaatc aacacttacc gaatcgagca ttgtgggatc gggcggaaag ccgtcgtgaa
     4261 gttgttcgcc atccacagag gatacggaga cacatcccga tgaatcagca atttcgatgc
     4321 ggtagttgat gaaggcgctg caaatagtaa ccgtatcgag gtaggtcaac tgattggttg
     4381 aatctaaaaa gttccacgat ccagccggaa attcacggta gatatgataa tactgtgaag
     4441 aactggtgaa caaggggttg tgcacaggat tccagttcaa ttgggcaatt gggcctccgc
     4501 cgatagctgt cacatttaga taaatggtgc tcactgtatc gctgggagcc gattggtaca
     4561 agccataaca tcccgaacgg gtagtcatat aaaaatactg ttgcccggcg tttccgccta
     4621 atccgctgta ggttttagtg gtctgattga tatcaaaaat actgtcgatt ttgacaaaag
     4681 gacctgcggg tgtggatgca tgatatacga aaaatccgtt aaatgaattc atggtatcaa
     4741 tgggaggaat ccagttcagg gtaatatcgc cgttgggatg cacacgcgtg catttcggct
     4801 ccggaggcgg taaaacggga gtaggcagca ccacaataga tatggtggct atttttatgc
     4861 caggcgccgg gcaaaaatca tcgtaaactt ttataacaaa attatgcacg ttgaacatgg
     4921 tcccgcaaat gccgggaatg ccggtgatag aagccgggtg tgtcaagtga tcgcacgtgg
     4981 ttcgccagtg aaaatgtgtg gaaacataca tcatggcaga taatgtcgga ggcggtgtga
     5041 gggtggcgca aggtggccgc ggacagccgg aggtttcgct cacataacca gcaccaaaat
     5101 cctgtccgga agcttccaga gtaaccgtct gcggattgtt attgggcagg acaccgtagt
     5161 caatacctgt gatatcaaca tccacggtat ctccggcata cactgtatcg gaaaattcat
     5221 agatgcccag gttagcatta taaaatggcc cggccacgga gggtggggtg ttagaccccg
     5281 gacaggcgag cagtacaatc tgcatttccc tgaaaatctc ggcaattttt actccgcaac
     5341 ggtaggccgt aactttagta actgtaacaa aagcgccact ggtataggag gtataggata
     5401 tttcgccatt gtacggattc atcgtagcag gtacgttcag cgggttttga gccgtgccgg
     5461 gcaagggact gttataagta tacgtgccca tataacccat attggtggta gcgttttgca
     5521 aggcaggagc ccaggaaaaa gccagagaat ccagctcttt atcggtagca ttatgattat
     5581 acttgaaagg gtatccggta caaataaccg tggaaggaac ttcggcaaat accgggctgt
     5641 tatcgtagca ggggttggta ttctgaccat tgtatggata cattatggcc ctcaggaacc
     5701 agtcttcgct ggtggggttt accatattag cgctgggatt acggcaacag ccgtcgttat
     5761 agaaaatcca gccgctcgtt ggaggcacgc cactaagtaa cacccctgca ggataagcga
     5821 catcggaggt aaaataccat tcttcaatgg ctcctgtgtt agctgttgaa ggcgtaggca
     5881 ggcatttaat ctcggaatca taattccagc aatccggtga gatatcggtt ttcccgtcca
     5941 ggataccatc atctatatca tgaggattag cgcctggttt cagggccatg gtaatctgag
     6001 tcagaccggg atagccctga atatgcataa tttcgcccgt gctataagtg attccgttgc
     6061 actcgcggta tagcttcata ataaacctga aacgaccgtt tgacaggcac tcccacgtaa
     6121 tctcacctcc catataatga gaggccaaca aattgccatc ggtaaacaac tgacttccaa
     6181 gtagtagaat tagtaaaaga aaagttttaa cgggttttat cattgtttta attttatctc
     6241 gtattattat gcaaagtaac gaaaaaactg acacattttc atcattttat caaaataaaa
     6301 atgccattca tactgaataa cggtatttat taaaaaaaaa tttggtaaac gaaaaagtat
     6361 agtgaattat tttcctgctt tgcccacaat cagctctaac atagatgact cctgaaggta
     6421 tttttgggat gtgtgcagca aatcttctgc agtaacggcg tttatggtat tcataaaatc
     6481 catataatac tttttataat taatcccata tttcagcagg cttttaaagc gatcggcaac
     6541 ttggtagggc ccgtcaaaag atctaagcac ggagccggcc atataccttt taaccagttc
     6601 caattcatcc tgtacaatta attccgtatt taatatcttt aattctttgt aaatttcgtc
     6661 aaccgccctt tggcatactt ccgctcctac ctctgaggca atcagaaata ccccggcctg
     6721 ttgaaaagac atcatcatcg aatatatccc ataggtatag cccttgtcct ctctgatatt
     6781 tttcataagc cgcgacccaa agtatcctcc aagaatagta ttcaaaatat ataacttcat
     6841 aaaatcctca tgcgtcttat tgattgaaat ttttccaatc ctaatggccg actgcacagc
     6901 cgagggcatt tcaataaact gtttttgctt tcccgggcat ataactgaag acgccctgtt
     6961 ttcacttttt tgctttcctt ttttggaata gccaaaaaaa taattcaaca ggggtaaaat
     7021 atctttgctc aatttgccgg aaactaccac gttgcatttt tcggcagtat atcgctggtc
     7081 gtgaaaagcc tgaagttttg cggcttgctg ccggtcaaaa tcggccgccg tagtgaggga
     7141 accataagga tgatgtttcc cgaaaagctg atggttaaaa tgcaacccgg ctacttcgtt
     7201 cactttgcgg ccatcgtcga taaaagcctg tttatcttta cgggtgtggg tttttaactc
     7261 atcatccgaa aattttgggt gattgataat ttcgtttaag acaggaagca catatttcag
     7321 gtgtttgttg agcaccgata aagaaacaac agcatgatcc ttggacacgg ttttgttgag
     7381 ccaggcgcca taatagtcga gtttttgtgc gatttcgaaa gaggaataac tcaacgtgcc
     7441 ttcggtgagc attttattgg tgtaaaaagc aagcaaaggt gcgggctggt gcaggtttcc
     7501 cgcctcgaag ataaaatcaa ttttcgtgat ggcggtttcg cccaggtcaa aaagatagac
     7561 aggaatatta ttaacgagct tttcatttcg cggctcggga atatgaagct tatgcgcaac
     7621 tttaagcgcc ggtggctgtt ttctgtttat catctgttct tttttctttt ggcggccgcc
     7681 atataataca tcacggaaca gttgctatcc accagcacct tgttggcagc ctcctgtata
     7741 tcggcggcag tgaccctgct gtaattttgc gcttctgaat ttaccaaatc aacatcgccc
     7801 agcaattccg ataccgccag tcttaaggcc cg
//
LOCUS       DAOSFP010000074         7780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8095_length_7780_cov_3.444790, whole genome shotgun sequence.
ACCESSION   DAOSFP010000074 DAOSFP010000000
VERSION     DAOSFP010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7780
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8095_length_7780_cov_3.444790"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2348
                     /locus_tag="PLI16_03375"
     CDS             <1..2348
                     /locus_tag="PLI16_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH83627.1"
                     /translation="TANITVTPTINTTYTVTGTTATGCTGNTTVTVSINPNPAISANA
                     SPTSICQGSSTTLTASGANTYVWMPGSLNGTTVSVSPSAITTYSVTGTDAAGCTGSTT
                     VTVSMNPNAVISATASPASICLGTSSTLTASGASTYTWMPGALSGTTVTVTPTVTTIY
                     TVSGTTAAGCTGANTITVTVKPLPTVTAVPNQTYCAGVTAPMTSLSGPLTGTTFAWSN
                     SNAAVGLGSGGPGNPPAFTATNTTASSITSTVTVTPTLNGCVGTVATYTITVNPSPVA
                     TVPLSFAVCHGDVVPASAFTSATTGAVFSWTNSQTSIGLAASGSDNVPSFTASNPGIT
                     PVAATIAVTATANSCTGTPATYTITVNPLPVITFSVFPQLCTTSPAFNLAQASPAGGT
                     YTGAGITGATFDPAVAGIGTHTVVYDYTNPVTGCSNTDSSTIEVMGALNITVTPNSPF
                     ICPENSILLMANGAYTFEWLPADGLSSTTGSNVVATPAAGTTYTVTGTNPDGCVGSTT
                     VTVGVYTIPVLAIFAEPKEGCSPLDVHFSYGPVGTIDTNTMLWNFGDLSSAGNTSAMA
                     NPSHIFNYNGNYPIYLTALTPDGCPVSAIDTVRAFIRPVADFYNNPEVAYSDNPQMTF
                     IELSSNAITWTWDFGDPGSYNYNSSNLRNPVHIFSDSGTYVVQLIVSSSQSCADTVSK
                     PVKIYPEILVYIPNAFTPNDDGLNEAFKPVFTGIDPNNYDFYIFDRWGNIQFHSNDPE
                     IPWDGKNNEKLCESGVYVYFIIYHSVTGKKFKLRGNVTLVR"
     gene            2411..2668
                     /locus_tag="PLI16_03380"
     CDS             2411..2668
                     /locus_tag="PLI16_03380"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOH83628.1"
                     /translation="MENNKTEFTVKGEQLLDKIKQLLHEGNIRRIIIKDVKGNVFMEI
                     PLTIGVVGTALLPVLAAVGALAALVSDFTIQIIKRDDPDIK"
     gene            2672..3502
                     /locus_tag="PLI16_03385"
     CDS             2672..3502
                     /locus_tag="PLI16_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH83629.1"
                     /translation="MEVNKIQDVEELRKISSNIRRNILRSLAASGSGHTGGSLGLADV
                     FTCLYFSVMNHNPQNPQWEDRDRLILSIGHVAPVLYCTLAQAGYFPEGELLTLRKLGS
                     RLQGHPGRDHGLPGIELSAGSLGQGLSVATGMALAAKYDKKNHKILCVMGDGELQEGS
                     VWEAAMSASHYHLDNLCVIVDRNKLQIDGSTSDVMEIEPLADKWQSFGWKVIACNGND
                     HEELLSSFSENEKSSGRPVVIIANTLMGKGVKAIENNYRWHGKAPSKEEAEQFIAALE
                     "
     gene            3517..4473
                     /locus_tag="PLI16_03390"
     CDS             3517..4473
                     /locus_tag="PLI16_03390"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOH83630.1"
                     /translation="MYFENRGNRATKAGFGQGVLEAGKLHPELMVLGADITASVGLDL
                     FAREFPDRFFSLGIAEQNCAGVAAGLALSGKIPVFSTYCVFAALRSADQIRVSLCYNN
                     LHVIIGGAHAGLSVGPDGATHQALEDIAIMRALPKMTVLSPCDATQATLLTKMAVKDC
                     KGPVYIRFGREAVPDFIPENIETTVGKGQLIKDGKDISIIATGHMVWEALQAAKMLEM
                     KGVKPRVINMHTIKPLDKEIIIHAARETKKIVTVEEHQAFGGFGSAVAEVVAQHCPVK
                     MKIIGVQDTFGESGQPDELMEKYGLNAESIFKEAMLLMNDSQ"
     gene            4481..5326
                     /locus_tag="PLI16_03395"
     CDS             4481..5326
                     /locus_tag="PLI16_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOH83631.1"
                     /translation="MKIFKGTGIFMAITLVLLCSYYIVISKNKAAAIQAKEEATVTRY
                     GTVEKLVPADTADNDTIVSVEFLTGKFDPAKEKSFTECDLEYANRSGIFIGQETYKAF
                     IKMFYAAKNDGVQLKIISGARNFRYQKSIWEQKWNGGRLVDGKNLATTVKDPVERARI
                     ILKYSSMPGTSRHHWGTDVDINSVDNKYFETAAGKKTYEWLCQNAHKFGFCQPFTALG
                     DKRQGGYQQEKWHWSYLTLAKTFLKEYLEKVTYDHITGFAGSQTAKDLKVIDNYVMSI
                     NKDCE"
     gene            complement(5531..5887)
                     /locus_tag="PLI16_03400"
     CDS             complement(5531..5887)
                     /locus_tag="PLI16_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83632.1"
                     /translation="MRKKKLAAFLGMVSIITILTSCKKLDLAVDVPDCLEKKIEQIKD
                     EPVRNPPAEVWQWKADGKTYYYITSDCCDQYNYLYDDGCNQICAPDGGISGLGDGNCP
                     DFSGSVEKVLVWKDKR"
     gene            complement(5895..6773)
                     /locus_tag="PLI16_03405"
     CDS             complement(5895..6773)
                     /locus_tag="PLI16_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83633.1"
                     /translation="MIRGLAYFQAEDFDNAKIDFDKLIRLDKKYYVLYSMWSSYLANS
                     GECDEAADYCRLALNNCHDRVTYFYAAQYYLNCEKADSALFFINKTLDSTRYIDAYLL
                     RASIKYELEDFEGYEKDLDTVIFDIDKVIKLIPYNMDLYQFKYQVLMSYDEFYSALQV
                     LDTMLLYQPSAETYLQKYYLCKRTAMDKEAEAALNKAIEMDSTNSHVITFMIETYTEQ
                     QKYSDVIKLCNKIIDQTLNKYGPSDVAYAYRKRGNAKYLSGKKAEGCEDIQKAASMGD
                     IDAKIIMEQTCKLIKK"
     gene            complement(7248..>7780)
                     /locus_tag="PLI16_03410"
     CDS             complement(7248..>7780)
                     /locus_tag="PLI16_03410"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HOH83634.1"
                     /translation="AYNLNSLLPDDIVIHQIIKVKEDAHARFSAVRRTYKYYINTCRT
                     PFGKEYEFFMHRSPDIALMNHACEILKEYKDFSCFSKSHTQVNNFFCTIFEAGWQQSE
                     SRYVFMISANRFLRNMVRAIVGTMLDVGLHKTDLEEFRNIIEKGSRSNAGMSVAAKGL
                     FLTRVEYPEDLFEKDE"
BASE COUNT         2179 a         1734 c         1688 g         2179 t
ORIGIN      
        1 ctactgctaa cataacagta acccctacta ttaataccac ttatactgtt acaggcacta
       61 ccgccaccgg gtgtaccggt aacactaccg ttactgtatc cattaatccc aatcctgcaa
      121 tttcagccaa tgcttcacca acgtccatct gtcagggaag ctcaaccacg ctgacagcct
      181 ccggagccaa cacttatgta tggatgccgg gatccctgaa cggcactacg gtgagtgtgt
      241 cgccatctgc aatcaccaca tattctgtta ccggaactga cgcggctgga tgtaccggct
      301 ccaccaccgt caccgtatca atgaatccta acgccgtaat ctcagccacg gcttcgccgg
      361 cgtccatctg cctggggacg tcttcgaccc tgacagcatc cggggcctcc acttatacct
      421 ggatgcccgg ggcacttagc ggaactacag ttacggttac accgacagta acaaccatat
      481 atacagtcag cggtaccaca gccgcaggat gtaccggcgc aaacactata accgttacgg
      541 tgaaaccgtt gcccacggtg accgcagttc ccaaccaaac ctattgtgcc ggtgttacag
      601 cacccatgac ttcactctcg gggccattga caggcacgac ctttgcctgg tccaacagca
      661 acgccgctgt aggcctcgga tcaggtggtc cgggcaatcc tcctgctttc acggctacca
      721 ataccaccgc atcttccata acatcgacag taactgttac accaacgctc aacggttgtg
      781 tgggcactgt agctacctat accatcaccg ttaacccgtc tccagtagca acagtacctt
      841 taagttttgc tgtttgtcac ggcgatgtag tgcccgcttc tgcctttacc agcgctacca
      901 caggcgcagt gttttcatgg acaaactctc agacctccat tggtctggca gccagcggat
      961 ctgataatgt gccatccttt acggcatcca atccggggat aactccagtg gccgcaacaa
     1021 tcgctgttac ggcgacagcc aattcctgca cgggtactcc tgccacttat accatcactg
     1081 tgaatccttt gccggtaatc acattttctg tttttccgca gttgtgcact acttcgccgg
     1141 cttttaacct ggcacaggca tctccggcag gaggcactta tacgggagcc ggtatcacag
     1201 gagctacctt tgatccggct gtcgctggca ttggtacaca tacggttgtt tatgactata
     1261 ccaatcctgt aaccggctgc agtaatacag attcttcaac cattgaggta atgggtgcgc
     1321 tgaatatcac ggtaacgccc aacagtccat ttatctgtcc ggaaaacagc atattgttga
     1381 tggccaacgg ggcgtatact tttgaatggc tcccggctga cggtttgtct tccaccacag
     1441 gttccaatgt ggtagctacg cctgctgcag ggaccaccta taccgttacc ggcaccaacc
     1501 ccgacggttg tgtgggatca actaccgtta ccgtgggcgt ttataccata cctgtgttag
     1561 ctatttttgc tgaaccaaaa gaaggatgta gtcctttgga tgtacatttc agttacggcc
     1621 cggtgggcac tatcgatacg aataccatgc tctggaattt tggggatctg tcatcggctg
     1681 gtaacacttc tgccatggca aatccttccc acattttcaa ttataacggc aattatccga
     1741 tttatctcac agcactgact cctgacggct gcccggtgtc ggctatcgat acggtcaggg
     1801 cttttatcag gcctgtcgcc gatttttata ataatcccga agttgcctat tccgataatc
     1861 cgcaaatgac ctttatagaa ctttcttcca acgctatcac atggacgtgg gattttggcg
     1921 atccgggatc atataactat aactcctcaa atcttagaaa tccggtgcac atattctccg
     1981 attcgggaac ctacgttgtg cagttgattg tttcaagcag ccagagctgt gctgataccg
     2041 tgtcaaagcc ggtaaagatt tatccggaaa tcctggtata tattcctaat gctttcacgc
     2101 ctaacgatga cggactaaat gaagcgttta agccggtttt tacaggaatt gaccctaaca
     2161 attacgactt ttatatattt gaccgctggg gcaacataca atttcattcc aacgaccctg
     2221 agataccctg ggatggcaag aataatgaaa aattatgcga gagcggggta tatgtgtatt
     2281 ttattattta tcatagcgtt accggtaaga aatttaaact taggggtaat gttaccttag
     2341 tcagataaac taccggctaa aaaaaagtgg tatttttatc tcgcggaata atacaaatat
     2401 tttatttgtt atggaaaaca ataaaaccga attcacggtt aagggtgaac aactgttaga
     2461 taagatcaaa caactgttac acgaaggcaa tattcgccgt atcattatca aggatgtgaa
     2521 aggcaatgtt tttatggaga taccgcttac cattggggtt gttggcacgg ccctacttcc
     2581 ggtgctggct gccgtagggg cgttggctgc cctggtgagt gattttacca tacagattat
     2641 caaaagagat gaccctgata taaaatagcg tatggaagta aataaaattc aggatgttga
     2701 ggaattgaga aagatttcct ccaatatccg caggaacatc cttcgcagtt tggctgcttc
     2761 gggttcaggg cataccggcg gttcgctggg cctggccgat gtttttacct gtttgtattt
     2821 ttcggtgatg aaccataatc cgcaaaatcc gcaatgggaa gatcgcgacc gcctgatatt
     2881 atcgataggc catgtagcac ctgtcttgta ttgcactctg gcacaggcag gatattttcc
     2941 cgaaggagaa ttgctgacat tacgcaagct gggttcgcgg ctgcagggcc atcccggcag
     3001 agatcatggc ctgccgggta ttgaattatc tgccggttcg ctgggacagg gcttgtcggt
     3061 tgctacaggt atggcactgg ctgcgaaata tgataaaaaa aatcacaaga ttttgtgtgt
     3121 catgggcgac ggagaactac aggaaggatc ggtatgggag gctgccatga gtgcatcgca
     3181 ttaccacctg gacaaccttt gtgttattgt ggatcgcaat aaactgcaga ttgacggcag
     3241 tacttctgat gtaatggaga ttgagcccct cgcagataaa tggcaaagtt tcggctggaa
     3301 agtcatagca tgtaatggta atgatcatga agaattatta tcatcgtttt ccgaaaatga
     3361 aaaatcttcg ggaaggcctg tggtgataat tgcaaatacc ctgatgggca aaggggtaaa
     3421 ggccatagaa aacaattatc gttggcatgg taaagcccca tcaaaagaag aagctgaaca
     3481 atttatagcg gcattggaat aaaaaaactt agaaaaatgt attttgagaa cagaggcaac
     3541 agggctacaa aagctggttt tggacaaggg gtactcgaag caggcaaact acatcccgaa
     3601 ctgatggtat tgggtgccga cattactgct tccgttggat tggatttgtt tgccagggaa
     3661 tttcctgacc gttttttttc attgggaatt gccgaacaga actgtgcagg tgtggctgcc
     3721 ggccttgctc tcagcggtaa gatacctgtt ttttctacct actgtgtttt tgctgccctt
     3781 cgctcggccg atcagatccg ggtttcgcta tgttataaca acttacatgt catcattggc
     3841 ggagcacatg cagggctttc tgtaggccct gacggcgcca cccatcaggc actggaagat
     3901 atagccatca tgcgggcact tcccaaaatg acggtgcttt cgccatgtga tgctacgcag
     3961 gcaaccctgc tcaccaaaat ggctgtaaaa gactgcaaag gaccggtata tatccgtttt
     4021 ggacgcgaag ccgtgcccga ttttatacca gaaaatatag aaaccacagt agggaagggc
     4081 cagctcataa aagatggcaa agatatcagt attatagcca ccggacacat ggtctgggag
     4141 gcactgcagg cagccaagat gctggagatg aaaggcgtta agccccgggt aatcaatatg
     4201 cataccataa agccgcttga taaggaaatt atcatacatg ccgccaggga aacaaaaaaa
     4261 attgttaccg tggaggagca tcaggcgttt ggtggctttg gcagcgccgt ggccgaagtg
     4321 gtggcgcagc attgcccggt aaagatgaag attatcgggg tacaggacac tttcggcgaa
     4381 tcaggccaac ctgatgaatt gatggagaaa tatggcttaa atgctgaaag tattttcaag
     4441 gaagccatgc tacttatgaa cgatagccaa tgatgatgtt atgaagattt ttaaagggac
     4501 cggaatattt atggcaataa ctcttgtgtt gctttgttct tattatatag taatcagtaa
     4561 aaataaggcc gcggcaatac aagctaagga ggaggccact gtgaccagat acggcacagt
     4621 agaaaagttg gtgcctgccg acaccgcaga taatgacacc attgtcagtg tggaatttct
     4681 aacaggaaaa tttgatccgg caaaggagaa atcgtttacg gaatgcgatc ttgaatatgc
     4741 taaccgtagc ggaattttta tcgggcaaga aacctacaag gcttttatca aaatgtttta
     4801 cgcagccaaa aacgatggag tgcaactgaa aattatctcg ggagctcgaa acttccgtta
     4861 tcagaaatca atatgggagc agaaatggaa tggtggcaga ctggtggatg gaaaaaatct
     4921 ggcaactact gtaaaagatc ctgtggaacg ggcgcgtatc atacttaagt acagttctat
     4981 gcccggaacc tcgcggcacc actggggaac cgatgtggac atcaacagtg ttgataacaa
     5041 atattttgag actgcggcgg ggaaaaaaac ttatgaatgg ctttgccaaa atgctcataa
     5101 attcgggttc tgccagcctt ttactgccct gggcgataaa cgtcagggcg ggtatcagca
     5161 agaaaagtgg cactggtcgt accttacgct ggctaaaact tttttaaagg aatacctgga
     5221 aaaagtaacg tatgatcata tcaccggttt tgcaggcagc cagacagcca aagatcttaa
     5281 ggtgatagac aactatgtga tgagtattaa taaggattgt gaatgatgtc ctgtcagtat
     5341 tattaaatca cactggcttt ttaaaatatt tgtttagcca tgctcattca atggggagct
     5401 tcccacatgg atcacgtgga tttatggaca atcattacat tcgaagattt ggggatggcc
     5461 cgcggtgccg ttgtatcagc accaacagcc ttaaaattta ggatatttca tatatggcaa
     5521 agaaaatctt ttacctttta tctttccata ccaggacttt ttccacggag cccgaaaaat
     5581 caggacaatt tccatcgcca agccctgaaa tgcctccatc aggcgcacag atttgattgc
     5641 atccatcatc ataaagataa ttatactgat cgcaacaatc agatgttata taataatacg
     5701 ttttaccatc tgctttccat tgccacactt cggcaggagg atttcttaca ggttcatcct
     5761 tgatttgttc tatttttttt tcaaggcaat caggcacatc aactgccagg tctagctttt
     5821 tgcatgatgt gagaatggtt attatcgaga ccatccctaa aaatgcggct aactttttct
     5881 ttctcatacc tatattattt tttgattaac ttacatgttt gttccataat gatctttgca
     5941 tctatgtccc ccatggatgc agctttttga atatcttcgc aaccttctgc ttttttacct
     6001 gataaatatt tcgcatttcc tcttttgcgg taagcatagg cgacgtcaga tggtccatat
     6061 ttattaagcg tttgatcgat tattttatta catagtttaa tcacgtcgct atatttttgt
     6121 tgttctgtat aagtttcgat cataaaagta atgacatgac tgttggtaga atccatttcg
     6181 attgccttat ttaatgcggc ttcagcctct ttatccatgg cagttctttt gcataaataa
     6241 tatttttgta aatatgtttc ggcagacggc tggtacaaaa gcatcgtatc caaaacctgc
     6301 aatgcgctat agaactcatc ataagacatg agcacctggt atttgaattg gtataagtcc
     6361 atgttatatg gaattagttt aattaccttg tcaatatcaa aaataacggt atccaaatcc
     6421 ttctcatacc cttcaaaatc ttccaattca tatttgatag aagccctaag caagtatgcg
     6481 tcaatatacc ttgtggaatc gagggtttta tttataaaga ataaagctga atcggccttt
     6541 tcacaattca aataatattg ggcagcataa aaataggtga cgcgatcgtg gcagttattt
     6601 agtgccagcc tgcaatagtc cgcggcttca tcacactctc cgctgtttgc caaataactg
     6661 ctccacatgg aatataacac ataatacttt ttatcaagtc ttatcagctt gtcgaaatct
     6721 atttttgcat tgtcaaagtc ttccgcctga aaataagcca accctcttat catcaataat
     6781 tcatttaaat ctgcgtatcc caggaagtga gtgtcctcag ctcttcaaat aactcgtcaa
     6841 ccttagtgaa agacgttgtc gagtcaataa atagcagaat attatacgga tcattagcaa
     6901 aatttgaaga tctggaactt ttatacttta ctacattaca ataataatca tcaatctgtg
     6961 cgaaattatc tatactttgt actcccaaaa tacttgatag ctggatggaa tcattcttaa
     7021 tataaatgtt aaaaagaata ctatcgtaat tatatcccgg tgcttgtctg gtataaagtg
     7081 cttttggaat atttggcacc aatatgttct gacataatcc aattattggt acaagaaagc
     7141 ataagataat tgaaactgtt ttttttattt gtaaatgatt cattacctga aaagttatgg
     7201 atttatcata tttgtcgctc aaataaatcg gcaaatcctt tttaatttta ctcatctttt
     7261 tcaaataaat cctcaggata ttcaaccctg gttaaaaaca atccctttgc cgctaccgac
     7321 atgccagcat tagaacgtga acccttttca atgatattcc tgaattcctc caggtcagtt
     7381 ttatgcaggc ccacatccag catggtgccg acgatggctc tgaccatgtt tcgcaaaaaa
     7441 cggttagccg aaatcataaa cacataccgg ctttcggatt gctgccaccc ggcctcgaaa
     7501 atcgtgcaaa agaaattatt tacctgtgtg tgcgacttcg aaaaacacga aaaatccttg
     7561 tattccttca aaatctcaca tgcgtggttc atcaaagcaa tatcaggact gcggtgcata
     7621 aaaaactcat attctttgcc aaaaggcgta cgacaggtgt tgatatagta tttataagtt
     7681 ctccgcactg ccgaaaaacg cgcatgggca tcttctttca ctttaatgat ctgatgtatg
     7741 acaatgtcat cgggcaagag gctgttcaga ttatatgccg
//
LOCUS       DAOSFP010000075         7754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8128_length_7754_cov_3.656839, whole genome shotgun sequence.
ACCESSION   DAOSFP010000075 DAOSFP010000000
VERSION     DAOSFP010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8128_length_7754_cov_3.656839"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..538
                     /locus_tag="PLI16_03415"
     CDS             293..538
                     /locus_tag="PLI16_03415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOH83635.1"
                     /translation="MNAYYLEKAKERMPNIPLLVNVVSKRVRQLNAGQRPLIKPDTLD
                     MDHLDLALKEIAEGKLSAELGALPTPQAEVGRSLLAL"
     gene            563..1141
                     /gene="smpB"
                     /locus_tag="PLI16_03420"
     CDS             563..1141
                     /gene="smpB"
                     /locus_tag="PLI16_03420"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOH83636.1"
                     /translation="MTSRQDAGKGALATNRKARHEYHILDSWEVGLSLTGGEVKSCRA
                     GHASLAEAYAAFENGELFIYSMRIQPYAAAASSFNTHEPDRPKKVLMHRRELDRLAGL
                     LQQKGHSLIPLKLYLARGKIKMELGLGKGKQLFDKREALRKKIHDRDNNIIYEYCKDG
                     KAGPISTKLYNRLLAIQYGDEADKFGWTHVVE"
     gene            complement(1189..2037)
                     /gene="pyrF"
                     /locus_tag="PLI16_03425"
     CDS             complement(1189..2037)
                     /gene="pyrF"
                     /locus_tag="PLI16_03425"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004375142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOH83637.1"
                     /translation="MNRKELISNIKKKRSFLCVGLDTDRKKIPEHLLDKEDPVFEFNK
                     SIISATIDYTVAYKPNIAFYEAMGVQGWISLQKTIDYLEPYRNEVFLIADAKRGDIGN
                     TSAQYAKTFFDKKISGFDFNAVTVAPYMGEDSVSPFLAHQNKWVVLLALTSNKGAADF
                     QYLQLAGENKKFLFQQVLETSSKWGTSDNMMFVVGATQAEMLREVRKIVPDHFLLVPG
                     VGAQGGSLQQVAEYGMNADCSLLVNASRSILYASAEKDFDTKAREEAKKLKEEMAQYL
                     ADYSKI"
     gene            complement(2052..3125)
                     /gene="prfA"
                     /locus_tag="PLI16_03430"
     CDS             complement(2052..3125)
                     /gene="prfA"
                     /locus_tag="PLI16_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOH83638.1"
                     /translation="MLDKLKAINDRWEEIGRQMNEPDVMTDVKRFIRLSKDYKELTPI
                     VEAYKRYDTIINHIASAKDVLYNEKDEDMRSLAKEEVDNLSEEKERLEEEIRVMLIPA
                     DPQDGKNAIVEIRAGTGGDEASIFAGDLYRMYVRFAEIKGWKTELVDLTEGTVGGYKE
                     IIFNINGENVYGQMKYESGVHRVQRVPKTEAQGRVHTSAASVVVLPEADEFDVELKDS
                     DIRKDTYCSSGPGGQSVNTTYSAVRLTHVPTGIVVTCQDEKSQVKNLEKAMSVLRSRI
                     YESEYQKYLDEISSKRKTMVSTGDRSAKIRTYNYPQSRVTDHRIHLTLYNLQTIMEGN
                     GVQELIDALQLAENAERLKAAGE"
     gene            complement(3164..4333)
                     /locus_tag="PLI16_03435"
     CDS             complement(3164..4333)
                     /locus_tag="PLI16_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOH83639.1"
                     /translation="MTDQEKYNQRGVSASKEDVHNSLKNISKGLFPRSFCKIIPDYFG
                     QDDAYCSLMHADGAGTKSSLAYLYWKETGDLSIWKGVAQDAIVMNTDDLLCVGATQNI
                     LLSSLIGRNKFLIPGEVISAIINGTEEVLQMMRDHGLSIYSTGGETADVGDLVRTIVV
                     DSVVSCRMKREEVITNEKIAHGDVIVGLSSCGQATYENEYNSGMGSNGLTSARHEVLH
                     KMYAQKYPESFDGLIPENLVYSGGYKLTDVAPGMPVNIGKALLSPTRTYTPLIIQILT
                     QFRNDIHGMIHCTGGAQTKILHFISNLHIVKNNMMAVPPLFRIIQEQASYDWKEMYKV
                     FNMGHRLEIYTTEEIARQIISLAQEFHIDAQVIGYCESAPKASLTIRSEFGDFDY"
     gene            complement(4406..6931)
                     /locus_tag="PLI16_03440"
     CDS             complement(4406..6931)
                     /locus_tag="PLI16_03440"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH83640.1"
                     /translation="MSKKIYLLGFVAFLFLLFVQATPSVAQRNYSSDADEAFETEQYT
                     VAIDLYKQAYNKIKGNKPEKGRVLFRLAECYRKTGNHKQAINWYTRVVKSNYPDPIAH
                     LYLADALKADEQYAEAIVEYKEYSKLAPSDTRGTSGVESCEYSIKWKDDPTRYVVENV
                     KKLNSKQSDFSPYWADKNYKALLFTSTRDGSTGKSTDGWTGQSFSDIYITTLDKKGAW
                     SEPLPADENINTDVNEGTPWLNKKANVIYFTRCPYLKKSKMGCQVYFAKKQGRGWSTP
                     DTLKLYDDLSMHIGHPALSEDELTIYFAADLPGGIGGKDIWVAKRTKKTKPFDKAVNL
                     GPTINTTGDEMFPYLRDESTLYFSSNGHEHLGLGGLDIFETKLEGGKWSKPENLKYPL
                     NSAGDDFGIIFKADKNEGFLTSNRKGGKGFDDLYSFYLPPLLFTLQGVVRDDSTKQII
                     PGALVRLEGSDGTIIEDSTDATGTYQFGKTQIRENTSYELNVSKDNYFAAVGRETTVG
                     LKSSKDLIHDFNLAPIPKAPIVLPDVLYVIGQWYLTPQAMDSLNWLVDVLNQNERWVI
                     ELSSHTDIRPIAMTNDTLSQRRARSAVHYIVDSMGIHPGRIFAKGYGANRPRILDRDK
                     TVFLDGAKYPQCKEKPFFFPKGTELTEAYIKSLKTTCEKEAAHQLNRRTEFTILSEDF
                     MATDLNDSTATNVVIQVNPMDNVLTILPSGSGTFEGRSVINGISSDFKYEADEEGMLI
                     SEKLVMRLLTEYRVTKSDFKDKDKAFKEDGSIVEDAVVTIKKMTIAKKTQEFLTAKVV
                     KDLTNDVIIGSKVLTKFGEYYIDDEKKQLIFGTAPVVPENPEK"
     gene            complement(7126..>7754)
                     /gene="secF"
                     /locus_tag="PLI16_03445"
     CDS             complement(7126..>7754)
                     /gene="secF"
                     /locus_tag="PLI16_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOH83641.1"
                     /translation="LSDKAGKLLGKLSSNKVGPTIADDIKLSSMYAIIIALFVIFVYI
                     AIRFKKWQYGLGGVISLTHDALIVISMYSLFWGILPFGMEVDQAFIAAILTIIGYSIN
                     DSVIIFDRIREYIGLYPKRDLKENMNAAMNSTLGRTINTSGTTVITLLAMFILGGEMI
                     RGFIFALLVGIVVGTYSSIFNATPLAYDFIMMQKKRKEKKELKLQAKK"
BASE COUNT         2034 a         1853 c         1598 g         2269 t
ORIGIN      
        1 cgattcaatc tgttgccggc gcaagggaat atcatcctgt tccttgcgga accgacgaat
       61 ctcctgatca caagtctgca aaaccaacaa ttgctcaatc gccttagcca ctcgcctgcc
      121 tcacctttct atgcggtcgt taaaagaact aaccctcctt atatacgccc gccgcccacc
      181 actggcaagt gttttacctt cgcattttcg ccattgactc ccccgccccg cctccctata
      241 gtgcgtcctc ttgttaattt gattgcaccc tttttgtatc tggaggaatc ccatgaacgc
      301 gtattatttg gaaaaagcca aggaacgaat gcccaacatt ccgttgctcg tcaacgtggt
      361 ttccaagcgc gtccgccaac tgaacgccgg ccagcgcccg ctgattaagc cggacacgct
      421 ggatatggac cacctcgacc tggcgctcaa ggaaattgcc gaaggcaagc tctccgccga
      481 actgggcgcg ctgccgactc cccaggcgga agtgggccgc tcgctcctcg ccctttaagc
      541 gcccccggcg ccccaccgcc ccatgacttc gcggcaagat gccggcaagg gcgctctcgc
      601 caccaatcga aaagcccgcc acgaatacca catcctcgac tcgtgggaag tcggcctctc
      661 cctcaccggc ggcgaagtca aaagctgccg cgccggccac gccagcctcg ccgaagccta
      721 cgccgccttc gaaaacggcg aactcttcat ctacagcatg cgcatccagc cctacgccgc
      781 cgccgcctcc agcttcaaca cccacgagcc tgaccgcccc aaaaaagtcc tcatgcaccg
      841 ccgcgaactc gaccgcctcg ccggcctcct gcaacaaaaa ggccacagcc tcattcccct
      901 caaactctat ctcgcccgcg gcaaaatcaa aatggaactc ggcctcggca aaggcaaaca
      961 actattcgac aaacgcgaag ccctccgcaa aaaaattcac gaccgcgaca acaacatcat
     1021 ctatgaatac tgtaaagacg gcaaagccgg ccccatctca accaaactgt acaacagatt
     1081 gcttgctatc caatatggcg acgaagccga caaattcggc tggacacacg ttgttgaata
     1141 aacaaaaaaa aagctccgaa aggagctttt tttttcacct ttcttcactt aaatttttga
     1201 ataatcagcc agatactgag ccatttcttc tttgagtttt ttggcttctt cacgtgcttt
     1261 tgtatcaaaa tctttttccg ccgatgcata taagatactt ctcgaagcat tgactaacag
     1321 gctgcaatcg gcattcatgc catattctgc cacctgctgt aaactgcctc cctgggctcc
     1381 cacaccggga accagcagga aatgatccgg tacaattttt cttacttctc tcaacatttc
     1441 ggcctgggtg gcgccaacca caaacatcat attgtcggag gtgccccatt ttgacgatgt
     1501 ttccaaaacc tgctggaaaa gaaatttttt attttcgccg gcaagctgta aatattgaaa
     1561 atcagcggca cccttgttgg atgtaagagc aagcaacact acccatttat tttgatgggc
     1621 aagaaaaggg cttacagaat cttctcccat atatggggca acagtaacag cattaaaatc
     1681 aaatcccgaa atttttttgt caaaaaatgt tttggcatat tgggccgatg tattgcctat
     1741 atcgccacgt ttggcatcag caattaaaaa cacctcgttg cggtagggtt cgagataatc
     1801 aatagttttc tgcagactta tccaaccctg aacgcccata gcctcataaa aggcaatatt
     1861 cggcttatat gccaccgtat aatcaatggt agcactgatg atactcttat tgaattcaaa
     1921 aaccggatct tccttatcca gcaggtgctc tggtattttc tttcgatcgg tgtctaatcc
     1981 tacacaaaga aaggatctct tttttttgat gttggatatt aattcttttc ggttcataag
     2041 tatggttttt attattcgcc ggccgctttg aggcgttcag cattttcggc cagttgcagg
     2101 gcatcaatca actcctgaac gccattacct tccataattg tttgcaggtt atacagggtg
     2161 agatgtatcc gatggtcggt aacccggctt tgagggtagt tataggtgcg aattttggca
     2221 gagcggtcgc cggtagagac catagttttt cgtttagacg aaatttcgtc aaggtatttt
     2281 tgatattccg attcatagat gcggctacga agcaccgaca tagctttttc gagattttta
     2341 acctgcgatt tctcgtcctg gcaggtaacc acgattccag tgggcacgtg ggtgagtcgt
     2401 acggcgctgt aagtcgtatt aaccgactgt ccgccggggc cggaagagca ataggtgtct
     2461 ttccgtatat cggaatcttt taattccaca tcaaactcat ccgcttcggg caacaccacc
     2521 accgaagctg ccgaagtgtg cacccgcccc tgtgcttcgg tttttgggac acgttgcaca
     2581 cgatggaccc ccgactcata tttcatttgt ccgtaaacat tttcgccgtt gatgttgaaa
     2641 atgatttcct tatagccacc aacagtccct tcggtaagat ctaccagttc ggttttccag
     2701 cctttgattt cggcaaacct gacatacata cggtacaggt cgcccgcaaa aattgatgcc
     2761 tcgtcgccac cggtaccggc acgaatctcg acgatggcgt tttttccatc ctgagggtct
     2821 gccggaataa gcattacacg tatttcttcc tccagccttt ccttttcttc tgaaaggttg
     2881 tccacctcct cttttgccag cgaccgcata tcctcgtcct tctcgttgta aagaacgtct
     2941 tttgcagaag caatatggtt tattatggta tcatatcttt tataggcctc cacaattggt
     3001 gtaagttctt tataatcttt actcaggcgg ataaaccttt taacatcggt catcacatcg
     3061 ggctcgttca tctgccggcc tatttcttcc cagcgatcat taatcgcctt gagtttatca
     3121 agcatatctt aaaaatttca caaaattaat aataatagcg cagctaataa tcaaagtcgc
     3181 caaattccga acgtatggtc agtgaagcct taggggcgga ctcgcaatag ccgataacct
     3241 gagcatctat atggaactcc tgggcgaggc ttattatctg ccgggcaatc tcttcagtgg
     3301 tataaatttc caggcggtgt cccatgttga acaccttata catttctttc cagtcgtaag
     3361 aggcttgttc ctgaataatc ctgaataacg gcggtaccgc catcatgttg tttttcacta
     3421 tgtgcaggtt ggagatgaaa tggagaattt ttgtttgtgc gccgccggta cagtgtatca
     3481 tcccgtgaat atcgttgcga aactgagtga gtatctgaat tatcagcggg gtataggtcc
     3541 tggtaggcga caacaacgct ttacctatat tcacaggcat tccgggggcc acatcagtaa
     3601 gcttataacc gcccgagtac acaaggtttt cggggatcaa tccatcgaaa ctttccggat
     3661 atttttgtgc atacatcttg tgcagcacct cgtgccgggc cgatgtgagt ccattgcttc
     3721 ccatgccgct gttgtactca ttttcgtaag tggcttgtcc gcatgatgac aaaccgacaa
     3781 ttacatcgcc gtgggcaatt ttttcattgg taatgacctc ctcacgtttc atcctgcagg
     3841 aaacaacgct atcaacaact atggtgcgca ccaggtcgcc cacatcggcg gtttcgcctc
     3901 ctgtggaata aattgacaat ccatggtcgc gcatcatctg taatacttct tccgtaccgt
     3961 tgataatagc cgaaatcact tcaccaggta taagaaattt attacggcca atgagagatg
     4021 ataatagaat attttgggtg gcgccaacac atagcagatc atcggtgttc attacaatgg
     4081 catcctgagc cacacctttc catattgaca aatcgcctgt ttccttccag taaaggtagg
     4141 ccagggatga ttttgtcccg gcaccatcgg catgcataag agagcaatac gcatcatcct
     4201 gaccaaaata atcaggaata attttacaga aagatcgcgg gaacaatcct tttgaaatgt
     4261 ttttcaaaga attatgtaca tcttcttttg aggcagatac accccgctga ttgtattttt
     4321 cctggtctgt catttcattt taataaaaaa agcatcccga tagctatcgg gacgcttttt
     4381 taaatattat ttattaatac aaatgttatt tttcaggatt ttccggaacg accggagcag
     4441 ttccgaaaat aagttgtttc ttttcatcat caatatagta ttcgccgaat ttggtcagca
     4501 ccttagaacc gataataaca tcattagtaa ggtccttcac caccttggcg gttaagaatt
     4561 cctgtgtttt tttggcgatg gtcattttct tgatggttac cacagcatct tcaacgatag
     4621 aaccatcttc cttgaaagct ttatccttat ctttaaaatc tgatttggta acacggtact
     4681 ctgtcagcag gcgcataacg agtttttcgg aaatgagcat accttcttca tcggcttcat
     4741 atttgaagtc ggatgaaata ccgttgatga cgcttcggcc ttcgaaagtg ccgctaccgc
     4801 tgggcaggat cgtaagcaca ttgtccatgg ggtttacctg aatgactacg ttagtagctg
     4861 tagaatcatt aaggtcggta gccataaagt cttcacttaa aatagtaaac tctgttcttc
     4921 tgttcaactg gtgcgcagct tctttttcgc aggtagtttt cagcgatttg atataagctt
     4981 ctgttaactc ggttcctttg gggaagaaga atggtttttc cttacattgc ggatatttgg
     5041 caccatcaag aaacactgtt ttgtcgcggt cgagtatgcg cggcctattg gcgccatagc
     5101 ctttggcaaa aattctcccc gggtgtatgc ccatagagtc aacaatataa tgaacagcag
     5161 accgcgccct gcgctgcgac agggtgtcgt tagtcatggc tattggcctg atatcggtat
     5221 gtgaagacaa ttcaataacc cagcgttcgt tttggttaag cacatccacc agccagttga
     5281 gcgagtccat tgcctgtggg gtaagatacc actgtccgat tacataaagt acgtcgggca
     5341 atacaatagg tgctttgggg ataggcgcca ggttaaaatc gtggataagg tctttgctgc
     5401 ttttcagtcc aactgtggtt tcgcggccga cagcagcaaa ataattatct ttagaaacgt
     5461 tgagttcgta agatgtattt tcacgtatct gggttttacc aaactggtag gttcctgttg
     5521 catcagtaga atcttctata atggttccat cagatccttc gagccgcact aaggctccgg
     5581 gaataatttg tttggtgctg tcgtctctga ctacgccctg aagagtaaat aataaggggg
     5641 gcagatagaa agaataaaga tcatcaaagc cctttccacc cttacggttt gatgttaaaa
     5701 acccttcgtt tttgtcagct ttgaaaataa taccaaaatc atcgccggct gaattcaggg
     5761 ggtatttcag gttttcgggt tttgaccatt taccgccttc aagtttggtt tcaaaaatat
     5821 cgagtccgcc aagtccaagg tgttcgtgtc cgttagagga gaaatacaaa gtactttcat
     5881 ccctaaggta aggaaacatt tcgtcacctg tagtattaat ggttggcccg aggttcacag
     5941 ccttatcgaa aggttttgtt tttttggtgc gcttagccac ccagatatct tttccgccta
     6001 ttccgccggg cagatcagca gcaaaataaa ttgtaagttc gtcctcgctc aatgcaggat
     6061 gtcctatatg catactcaga tcgtcgtaca gtttgagtgt gtcgggtgtg gaccatccgc
     6121 ggccttgttt tttggcaaaa tatacctggc agcccatttt ggatttcttg agataggggc
     6181 accttgtaaa ataaataaca ttggcttttt tgtttaacca gggagttcct tcattaacat
     6241 cggtattgat attttcatca gccggcaacg gttcgctcca ggcacctttt ttatctaatg
     6301 tagttatata aatgtcggaa aaactctggc ctgtccatcc gtcggttgat ttgccggtag
     6361 agccatcgcg agttgaggta aataataatg ctttatagtt tttatcggcc caatatggtg
     6421 aaaaatcaga ttgtttactg ttaagttttt ttacattttc aaccacatac cgggtggggt
     6481 catctttcca ttttattgaa tattcgcatg attccacacc ggatgttccg cgggtatcgc
     6541 tgggggccag cttgctgtat tccttatatt ctacaattgc ctcggcatat tgttcatcag
     6601 ctttcagagc atctgcaaga tacaaatgag ctataggatc cgggtaattg gatttaacaa
     6661 cccgggtgta ccagtttata gcttgtttgt ggttgccggt ttttcggtaa cattctgcca
     6721 aacgaaacaa tactcttcct ttttcgggtt tatttccttt aattttgtta taggcctgtt
     6781 tatacaaatc aatagcaaca gtatattgtt ctgtttcgaa cgcctcatcg gcgtcggatg
     6841 aataattacg ctgcgcaaca gatggcgtgg cctgcacaaa taagagaaat aaaaatgcaa
     6901 cgaagcctaa tagatatatt ttcttgctca tataaaataa tattaaaccc taataatagt
     6961 ttgctaattt aaaaaaataa ttaacactaa aaaaaagaac atttatatat tttttaaaaa
     7021 aaatactaca atgcttatta tcaactaatt agcattgtaa tattttctta tcaatatttt
     7081 tctttggctg agatttctta actttttaaa ttttttaatt atacgttatt tctttgcctg
     7141 aagtttcaat tctttctttt ctttacgttt tttctgcatc ataataaaat cgtaggccag
     7201 aggggtagca ttgaaaatag aagaatatgt acctactaca ataccaacca ataaggcaaa
     7261 aataaatccc ctgatcattt cgccacccaa aataaacatg gcgagcaagg taatcactgt
     7321 cgttcctgag gtattaatgg ttcggcccag agtactattc atagcggcat tcatattttc
     7381 cttcaggtcg cgtttgggat aaagcccgat atattccctg attcggtcga aaatgattac
     7441 ggagtcgtta atagagtacc cgataatagt aagaatagcg gcaataaacg cctggtcgac
     7501 ctccatgccg aaaggtagta ttccccagaa cagcgaatac attgatatta ctattaaagc
     7561 atcgtgggtt aatgatatca caccacctaa tccgtattgc cattttttaa atctgatggc
     7621 aatgtaaaca aatattacaa aaagagctat gatgatggcg tacatggacg ataatttgat
     7681 atcatcagct atagtgggcc ccactttatt ggaacttaat ttccccaata gtttaccggc
     7741 tttatcactc agga
//
LOCUS       DAOSFP010000076         7742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8144_length_7742_cov_4.410693, whole genome shotgun sequence.
ACCESSION   DAOSFP010000076 DAOSFP010000000
VERSION     DAOSFP010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8144_length_7742_cov_4.410693"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2769)
                     /locus_tag="PLI16_03450"
     CDS             complement(<1..2769)
                     /locus_tag="PLI16_03450"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOH83642.1"
                     /translation="MLKNFKLLNNILGWIVFVVASGVYLLTIEPTASWWDCGEYISTA
                     FKLEVGHPPGAPLFQLMGRFFSLFAGGDVTKVAMMVNSMSAILSGFAILFLFWSITAI
                     AKKLFVKSSSYDLTVPQTLIVLGAGLVGSLAYTFTDSFWFSAVEGEVYATSAFFTAVV
                     FWAILKWEVVADEKYANRWIVFIAFMMGLSIGVHLLNLLCIPAMTMLYYFRKGKQSTK
                     NFILVLILSVIILGLVQSGIIPWTVKLAGYFELFFVNTLHLPFNSGTIIYFVLLIGLI
                     AFGLIYSKKHGKTILNTVILCFTFIIIGYSSFVVLVVRANANTPINENAPKDALTLLT
                     YLNRDQYGDWPVLYGQYFNARVVDTKEGAYNYIKGEDEYIPTTRKVSYQYDPQGCGLF
                     PRMWSEQDKHIRAYRAWTGTKGKTKPTFGENLAFFFKNQIGNQYLRYFMWNFVGRQND
                     IQGFGIDEAGNKDVANGNWISGIKFLDAAHLGNQDKLTDKMLTNKGRNTYYFLPLLLG
                     LAGLAFHFWKNKKDAFVVTLLFLLTGLAIIVYLNPTPYQPRERDYAYAGSFYAFAIWI
                     GIGVVALYHGLSKKIPQIASVIIAVVASFLLVPTIMAKENWDDHDRSDKYACRDYAKN
                     YLASCAPNAILITNGDNDTFPLWYVQEVEGFRTDVRVVNFTLASGDWYIHQLFNKIYE
                     SAPLPFTIPSHKYTAGTNDFVPYYEKDANNVWDLKKLINFINSDKEDSKVQLQDKRRV
                     AYMPTKNLSLRVDSAKVCNNGTVPRYLADRVVKAITWKVNKNTLYKNDLMLLDLIATN
                     NWERPIYFASPSSVEDFINITDYCFLDGCVYRFLPVKGDARRGGVLTNETYDCLMNKF
                     VYGNINDPTIYVDKESYGMSLYLRNNFARVAQGLLAEGQKAKAIKALDRGAELLPDYA
                     VPYDLYMIG"
     gene            2876..3658
                     /locus_tag="PLI16_03455"
     CDS             2876..3658
                     /locus_tag="PLI16_03455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH83643.1"
                     /translation="MDTLFRLKDFLRYRHKAGTAFKVHSPFAYSFYTEVVRSKQKFPA
                     ALLAAEQIRKTCLHSPEKIFVNDLGTGGVSLPQERKIKDIMRRYACSKKEAFLLYRMI
                     NYLKPVTILELGTSLGISTIYMALANQDIALTSLEGCPQTAAAARKNFEKLSLNINLI
                     VGNFDETLPVALKKMQVAGLVFFDGNHTKEATLRYFDECLKHTNNQSVFVFDDIHWSP
                     GMYDAWTTICANPEVTTSMDLYQLGIVFFNKKISKQHFVLKY"
     gene            3689..4432
                     /locus_tag="PLI16_03460"
     CDS             3689..4432
                     /locus_tag="PLI16_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH83644.1"
                     /translation="MKQEIIRLTNISRHYKVGTHLVKALQDISLTIYKNEYVALMGPS
                     GSGKSTLMNILGCLDTASGGTYILNGKDVSKLDDNELAEIRNKEIGFVFQTFNLLPRS
                     TALDNVTLPLVYAGYGKNQRTERAMQVLEQVQLADRMTHKPNELSGGQRQRVAIARAL
                     INKPSIILADEPTGNLDSKTSIEIMGLFEEIHEQGNTIILVTHEEDIARHAHRIIRLK
                     DGLVDTDLPNENFRKMEFYDRENISEEEE"
     gene            4429..4992
                     /locus_tag="PLI16_03465"
     CDS             4429..4992
                     /locus_tag="PLI16_03465"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOH83645.1"
                     /translation="MKATKTKIYTKKGDAGQTSLLGGERVDKADPRLEAYGTLDELNS
                     YIGLVRDFAIDIPVKRFLLSIQEQLFTAGSILAAGNDKVLSKIPSINKDDLLALEREI
                     DRMCEKIPPQKQFILPGGHPIISYCHIARCICRRAERAIARLDLLEKKQRLTLTYVNR
                     LSDYLFVLARFLAAELGIKENFLENTK"
     gene            5173..5394
                     /locus_tag="PLI16_03470"
     CDS             5173..5394
                     /locus_tag="PLI16_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOH83646.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAMRTGLPSEVIENLKEIE
                     DEGEIYESIEDIWPDYPTKEDFFFHEDEY"
     gene            5520..6515
                     /gene="trpS"
                     /locus_tag="PLI16_03475"
     CDS             5520..6515
                     /gene="trpS"
                     /locus_tag="PLI16_03475"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOH83647.1"
                     /translation="MEIVVSGIRPTGNLHLGNYLGALQNFVKMQEENNCYFFIADYHS
                     LTTHPTPADLHGNVRNVLVEYLASGLDPEKATIYIQSDVPETAELYLILNMNAYKGEL
                     ERCTSFKEKARTQPENINAGLLTYPTLMAADIIIHKAAMVPVGKDQEQHLEMTRTFAK
                     RFNRLYNVDYFPEPVAYNFGKNLIKIPGLDGSQKMSKSDNENSCIFLADTADMIRKKV
                     MKAVTDAGPTAPNSPKPQVIENLFSLMKVVSTEDTIKHFDEQYNNCSIRYGDLKKQLA
                     EDMIAFVSPITERIKALASDSDYLHKVACMGAEKARASAAKTVKEVREIIGFKKF"
     gene            complement(6552..6815)
                     /locus_tag="PLI16_03480"
     CDS             complement(6552..6815)
                     /locus_tag="PLI16_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOH83648.1"
                     /translation="MSKLVLDLHEIYNKGRLIDKALNDIISEAVDKKIDWVEIIPGKG
                     SGQLKKKVLRFLDTSEIKQQYHRIEKDRDNFGKIVVYFRHKKR"
     gene            6888..7232
                     /locus_tag="PLI16_03485"
     CDS             6888..7232
                     /locus_tag="PLI16_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83649.1"
                     /translation="MEPVKAFFSLSTWGLRLAVLVLIYIIFYGTLSTLEYKNTDFLIA
                     AGFALFGILLFIGGFMNKSTMTILSSIILMLGCAYEIVMHYGFQKGRFVAIYFVLGAI
                     TLFFFANGNKKK"
     gene            7295..>7742
                     /locus_tag="PLI16_03490"
     CDS             7295..>7742
                     /locus_tag="PLI16_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOH83650.1"
                     /translation="MNPPDNTKERAILIGLITPTQNKEKALVYLDELSFLLKTAGGEV
                     VEKFLQKIDRPDPKSYVGSGKIEEIRNYVQENNINLAVFDDELSPSQIRNIEAALKCR
                     VIDRNNLILDIFAFRARTSYARTQVELAQYQYLIPRLTGMWTHLEKQ"
BASE COUNT         2375 a         1725 c         1585 g         2057 t
ORIGIN      
        1 cccaatcatg tataaatcgt agggcactgc atagtcggga agcaactcag cacccctgtc
       61 aagcgcctta atggcttttg ctttttgtcc ctcggccaac aatccctgtg ccacgcgggc
      121 aaaattattt ctcagataca acgacatacc ataactttct ttgtccacat aaattgtagg
      181 atcgttaatg ttgccataaa caaatttatt catgaggcag tcgtaggtct cattggtaag
      241 cacaccaccc ctgcgggcat cgcctttcac aggcaaaaaa cggtaaacgc agccatcaag
      301 gaaacaataa tcggtgatat tgataaaatc ttcaacactc gatgggctgg caaaatatat
      361 ggggcgttcc cagttgttgg tggcaataag gtcaagcagc atcaggtcgt ttttatataa
      421 agtgttttta ttgactttcc aggtgatggc ttttaccacc ctgtcggcca gataccgcgg
      481 caccgtgcca ttattgcaca ccttggcaga atcgacacgg agcgaaagat ttttggtagg
      541 catataagct accctgcgtt tatcctgcag ttgcacttta ctgtcttcct tatcactatt
      601 gatgaaatta atcagttttt tcaaatccca gacattattg gcatctttct cgtaataagg
      661 gacaaagtcg ttggtccctg ctgtatattt atgcgaaggt atagtgaaag gcaggggggc
      721 tgattcataa attttgttga acagttgatg aatataccag tcaccggagg ccaaggtaaa
      781 attcactacc ctgacatcgg tacgaaaacc ttcaacctcc tgaacatacc atagggggaa
      841 agtgtcgtta tcaccattgg taatcaggat ggcattcggt gcacatgaag ccaagtaatt
      901 ttttgcataa tcgcggcagg catatttatc ggagcggtcg tgatcatccc agttttcctt
      961 ggccatgatg gtgggaacca gcaaaaaact ggccacaaca gcaattatca cgctggcaat
     1021 ctgtggaatt tttttggaca gcccgtggta taatgccacc acacctatcc ctatccagat
     1081 tgcgaaggca taaaacgatc ctgcataggc ataatcacgt tcgcgcggct gatagggtgt
     1141 aggattcagg taaacgatga tggccagtcc tgtaagcaaa aaaagcaaag tgaccacaaa
     1201 ggcatctttt ttgtttttcc agaaatggaa tgccagtcct gccaatccca gcaacagcgg
     1261 caggaaataa taagtattcc ggcctttatt ggttaacatt ttgtcggtaa gtttatcctg
     1321 attacccaag tgggcagcat ctaaaaactt gataccactt atccagttgc cattggcaac
     1381 atccttgttt ccggcttcat cgatgccaaa gccctggata tcgttttgtc gtcctacaaa
     1441 attccacata aaatagcgca ggtactgatt tcctatctgg tttttaaaga aaaacgccag
     1501 attttcgccg aaggtaggtt ttgttttacc cttggtcccc gtccacgccc tgtacgcccg
     1561 gatatgtttg tcctgttcac tccacatacg cgggaaaaga ccgcagccct gaggatcata
     1621 ttgataggaa actttccgtg tggtgggaat gtactcgtct tcacccttaa tgtagttata
     1681 agctccttct ttggtatcga ccacgcgggc attgaaatac tgcccgtata gtacgggcca
     1741 gtcgccatat tgatcgcgat tcagataagt cagcaaggta agggcatcct tgggggcgtt
     1801 ttcattaatg ggagtgttgg cattagcgcg aaccaccagc acaacgaatg aagaataacc
     1861 gataataata aaggtaaaac ataaaatgac ggtatttaag atagttttac cgtgtttttt
     1921 tgaataaatc aggccaaaag caatcagacc aataagaagc acaaaataga taatagttcc
     1981 tgaattaaaa ggcaggtgaa gagtatttac aaaaaacaat tcgaaatatc cggccagttt
     2041 aaccgtccag ggaataatgc ccgattgaac caatccaaga attattacag aaaggataag
     2101 cacgaggata aaatttttgg tagattgttt gccttttctg aaataataaa gcattgtcat
     2161 ggcggggatg caaagcaggt tgagcaagtg aactccgatg gacaagccca tcataaaggc
     2221 gatgaacact atccagcggt tggcgtattt ttcgtcggcc accacttccc atttcaaaat
     2281 tgcccagaaa acgacggcgg tgaaaaatgc agacgtggca taaacctcgc cttcaacagc
     2341 agagaaccag aaagaatcgg taaaagtata tgctaaagag ccaaccaggc ctgctcccag
     2401 tacgatgagt gtctgtggta cggtaagatc gtaactgctg ctttttacaa acaacttttt
     2461 ggcaatggcg gtgatggacc aaaacaaaaa caagattgca aaaccgctta atatagccga
     2521 catactgttg accatcatgg ccaccttagt aacatcgccg ccggcaaaca gcgaaaagaa
     2581 tcttcccatc aactgaaaca agggtgctcc cgggggatgg cccacttcaa gtttaaaagc
     2641 agttgagata tattcgccac aatcccacca actggccgta ggctcgatgg tgagcaaata
     2701 aacgccagag gccaccacaa atactatcca gccaaggata ttgttaagta acttaaagtt
     2761 tttcagcatg ttcttaggtt tttcattatt attttaatcg acgaaattag taattatgac
     2821 attatatagc tgcatttttt agaaaaaaat tacttttacc gcaacaaatc atcttatgga
     2881 cactcttttc cggcttaaag attttttacg ataccggcac aaggcaggaa cagcatttaa
     2941 ggtacactcg ccttttgcct attcctttta tactgaggtg gtacgttcaa aacaaaaatt
     3001 tcctgcagca ttgcttgccg cagagcaaat caggaaaaca tgcctgcatt cgccggaaaa
     3061 gatttttgta aatgatctgg ggacaggtgg ggtatctctg ccgcaggaaa gaaaaatcaa
     3121 ggatataatg cggcggtatg cctgttcaaa aaaggaagct tttctgcttt accgaatgat
     3181 caactacctt aaacccgtca ccatccttga attgggcacc tcgttgggaa taagcaccat
     3241 ctatatggcc ctggctaatc aggatatcgc cctgacctcc cttgagggat gtccccaaac
     3301 ggccgccgct gcccgcaaga atttcgaaaa actttcgtta aacatcaatc ttatcgtggg
     3361 aaattttgat gagacattgc ctgtggcatt aaaaaaaatg caagttgccg gattggtttt
     3421 ttttgatgga aaccacacca aggaagccac attaagatat tttgacgaat gcctgaaaca
     3481 taccaacaac cagtcggtat ttgtttttga cgacatccac tggtcacccg gcatgtatga
     3541 tgcatggacc acaatatgcg caaatcctga ggttaccacc agcatggatt tgtaccaatt
     3601 gggaatagtt ttttttaata aaaaaatcag taaacaacat tttgttttga agtattaaac
     3661 tacctttgtt tattattact ttcattaaat gaagcaggaa atcatcaggc ttacaaatat
     3721 ttcgagacat tataaggtag gcacccacct tgtaaaagca cttcaggata tttcgctgac
     3781 aatttacaaa aacgaatatg ttgccctgat gggaccttcg ggttccggca aatcaaccct
     3841 gatgaacatt ctgggctgcc tcgatactgc ttcaggaggc acctatattt tgaatggaaa
     3901 agatgtaagt aaattggacg acaatgagct ggccgaaatc cgcaacaaag aaattgggtt
     3961 cgtgtttcag accttcaatt tgctgcctcg ttccactgcc cttgataatg ttaccttgcc
     4021 cctggtatat gccgggtacg gtaaaaacca aaggacagaa cgcgccatgc aggttttgga
     4081 acaggtgcag cttgccgacc gcatgacaca taagcccaac gaactgtcgg gcggacaaag
     4141 gcaacgcgtt gctatcgccc gggcattgat aaacaagcca tccatcatac tcgccgacga
     4201 gcccaccggt aacctggatt caaaaacttc cattgagatt atggggcttt ttgaagaaat
     4261 acatgaacag gggaatacca tcatcctggt tacccatgaa gaagatatag cccgtcatgc
     4321 acaccgtatc atcagattga aagacgggct ggtggacacc gacctgccca acgaaaactt
     4381 cagaaaaatg gaattttatg acagggagaa catttcagaa gaagaggaat gaaagcaaca
     4441 aaaacaaaaa tatatacaaa aaaaggtgat gccggacaaa cttcgctatt gggtggagag
     4501 cgggtggaca aggcagaccc cagacttgaa gcctacggca ccctggatga attaaattct
     4561 tatattggtt tggtacgcga tttcgccatt gacatccctg tgaaaagatt tttactaagc
     4621 atacaggaac aactttttac agccggctct attcttgcag ccggaaacga caaagtttta
     4681 agcaaaatcc cctcaatcaa caaagacgac ctccttgccc tggaaaggga gattgaccgc
     4741 atgtgcgaaa aaattcctcc ccaaaaacaa tttatattac ccggcggaca tccgatcatt
     4801 tcatactgcc atattgcccg ctgtatatgc cgcagagcgg aacgtgccat cgcccggctc
     4861 gacctgttgg aaaaaaaaca acggctgacg ctgacttacg ttaatcgctt atccgactat
     4921 ttatttgtcc tcgcccgttt tcttgctgcc gaacttggca taaaagaaaa ctttcttgaa
     4981 aatacaaaat aattttaccc ttactcaata ttttgcacaa tattctcgtt atgcttgaaa
     5041 tactggggtt attgactatt tttatccggg aataatttgt taataaataa aaataattaa
     5101 ggataacaaa ataatacttg actttttaat atcaattatt atatttttgc aaaaaatttt
     5161 aaaccaggaa atatgtattg gactttagag ttagcatcaa aattagaaga tgcgccctgg
     5221 ccggcaacca aagacgagtt gattgattac gccatgcgga caggattgcc atcagaagtt
     5281 attgaaaatc ttaaagaaat tgaagatgag ggcgagattt acgaaagcat tgaggatatc
     5341 tggcccgatt atccaaccaa ggaagatttt ttctttcacg aagacgaata ttaataattc
     5401 ttaaattaac cataataaaa agcttccgga agggagcttt tttgttttta ggcaacaagt
     5461 tttctggaaa aattcacaaa gctgtgtaac tttgcagtgt ttcaccttat taaaacatta
     5521 tggaaattgt tgttagcggc atcaggccta ccggaaacct tcacctgggt aattacctgg
     5581 gcgctttaca gaactttgta aaaatgcagg aagaaaataa ctgctacttt tttattgccg
     5641 attaccattc gcttaccaca catcccactc ctgctgacct tcacggcaat gtccgcaatg
     5701 tgctggtaga ataccttgcc agtggcctcg atcccgaaaa agccaccata tatattcaaa
     5761 gtgatgtgcc ggaaaccgct gaattatacc ttattctcaa catgaatgct tataagggtg
     5821 aactggaacg ctgtacatcg tttaaagaaa aagcacgtac ccaaccggaa aatatcaacg
     5881 caggcctgct cacctatccc accctgatgg ctgctgatat tatcatccac aaagccgcca
     5941 tggtgcccgt gggcaaggac caggaacagc atctcgaaat gacacggacc tttgccaaac
     6001 ggtttaacag gctttataat gtggactatt ttcccgaacc tgtggcgtat aatttcggaa
     6061 aaaaccttat taaaattccc ggattagacg gcagccagaa aatgagtaaa tcggacaacg
     6121 aaaattcctg tatttttctg gccgacaccg cagacatgat acggaaaaaa gtgatgaaag
     6181 ccgttaccga tgcaggcccc actgcgccaa actcgcctaa gcctcaagtc atcgaaaacc
     6241 tgttcagcct gatgaaggtg gtctcaaccg aagacactat caaacacttt gacgaacagt
     6301 acaataattg cagcatacgt tatggcgacc tgaaaaaaca attggccgaa gatatgatag
     6361 cctttgtgtc tccgattacg gaaagaatca aagcgctggc ctccgatagc gattacctgc
     6421 acaaggttgc ctgtatgggg gcagaaaaag cacgggcaag cgctgccaaa acagttaaag
     6481 aagtcagaga aattattgga ttcaaaaagt tctaaggcta tttctcacgg gcaccaaaac
     6541 atcaattaca attatcgctt tttgtgccgg aaataaacca ctatttttcc gaaattatcc
     6601 ctgtctttct caattcggtg atactgttgt tttatttcac tggtgtctaa aaaacgcaat
     6661 actttctttt taagctgacc gctgcccttg ccgggaatta tttccaccca gtctattttt
     6721 ttgtctaccg cctcactgat aatatcgttg agcgccttat caataaggcg acctttatta
     6781 tatatttcat gcaaatccaa tacaagtttc gacatggcgg caaggatttt caacaaaggt
     6841 aaaccaatgt aaccaagttt aattaatttt gttctaaaaa taaaactatg gaaccggtaa
     6901 aagccttttt ttcattatca acgtggggat tgagattagc tgttttggtg ctgatatata
     6961 ttattttcta cggcactctg agcactcttg aatacaaaaa taccgacttt ctgatagctg
     7021 ccggatttgc acttttcggc atacttttat ttatcggtgg ctttatgaat aaaagcacca
     7081 tgaccatcct ctcttcgatt atccttatgc tgggttgtgc ttatgaaata gtgatgcatt
     7141 acgggtttca gaaaggccgg tttgtggcta tttactttgt tttgggcgcg atcaccctgt
     7201 tcttttttgc aaatggcaat aaaaagaaat aaacaaaccg ataccaactt aagtccctat
     7261 ccatctaaac ctccaacctc ctaacctccg ccccttgaac cctcccgaca acaccaaaga
     7321 acgtgcaatc cttatcggac taataacgcc aacacaaaat aaagaaaaag ccctggttta
     7381 cctcgacgaa ctgtcctttt tgctaaaaac ggcaggaggc gaagtcgtag aaaaatttct
     7441 gcagaaaatc gatcgtccgg atccgaaaag ctatgtgggg tcaggcaaaa tagaagaaat
     7501 cagaaattat gtacaggaaa acaacatcaa tctggccgtg ttcgacgacg agctctcccc
     7561 ttcgcaaatc cgcaatattg aagcggcact caaatgcagg gtaatagacc gcaacaatct
     7621 tattcttgat atttttgctt ttcgggcacg cacctcgtat gcacgcacac aggtagaact
     7681 ggcacaatac cagtatctga tcccccgcct gaccggcatg tggacacacc tcgaaaaaca
     7741 ac
//
LOCUS       DAOSFP010000077         7643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8279_length_7643_cov_3.673564, whole genome shotgun sequence.
ACCESSION   DAOSFP010000077 DAOSFP010000000
VERSION     DAOSFP010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8279_length_7643_cov_3.673564"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLI16_03495"
     CDS             <1..863
                     /locus_tag="PLI16_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH83651.1"
                     /translation="VSDAIRRSRAGLQDSKKPIGSFLFMGTTGVGKTELAKTLAEYLF
                     SDENALVRIDMSEYQERHTVSRLVGAPPGYVGYEESGQLTEAVRRKPFSVVLLDEIEK
                     AHPDVFNILLQVLDDGRLTDNKGRTADFRNTIIIMTSNIGSHVIQEKFAGINDENRDR
                     IIDNTRSEIISLLKQTIRPEFLNRIDEIIMFRPLSREEIKQIVSLQLSGVQKMLEQNG
                     IHLTVTEAALSKIAIMGYDPNYGARPVKRVIQQEVLNKLATQIISGAVQKDSEVELRL
                     KNDDFEFVNL"
     gene            1123..1578
                     /locus_tag="PLI16_03500"
     CDS             1123..1578
                     /locus_tag="PLI16_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOH83652.1"
                     /translation="MGKMYSAGNKKEIQQNKAKKRQEKEKKRLERKENSRQGGGLDDM
                     IAYVDEFGNITSEPQDLSKREELNIDDIAIKTQRKNDAEPQNKIRTGIVTFFNESKGF
                     GFIKDIDTQESIFFHMKGLLEPVKENNKVIFEVEKGVKGLNAFNVKIDR"
     gene            2139..4430
                     /locus_tag="PLI16_03505"
     CDS             2139..4430
                     /locus_tag="PLI16_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH83653.1"
                     /translation="MKKILFILFLCFSMHICTRSQQIALPQTNATNAFLSLPASIENI
                     DSSPIKKAVHDSLIGDVLTDLNADSIMSYIQHLQAYGTRFLLAPGHKDISLWLQHKLI
                     SFGYTNAVLDSFEITVEWPVGSGNMITNIQYNVIAELSGLISPDVQYVIGAHYDDILQ
                     FGGHDPYLVCPGADDNASGTAAVMEIARVLKERNFSSPATLRFVFFDAEELGTFGSRH
                     FVEDIMAGIEKPEIMFNLDMVGHEPADTAWLLHLNNYVGNEWICSMGAEIAAEFTQLG
                     TFNLFFNEPIGSDSDPFFLAGLPAIFLQEDDFSPYYHTLADVDSTINGLYCREIARLA
                     CAMLMNSSLVPTLVDFEISNPGDGHSLVPRWRPNPENNIAGYRVYFGTLPSTYDTVYA
                     TADTSLIFSGLSTDTLYYISVSAVNTEGREGIKNELNDRPAIVNLDQGILIVDDSEGG
                     FFDPPDSVVDAYYRYLCSDFNVTEYDATQNGPLTLAGLGPYSSVVWHINRANIIPKLR
                     SSLPELRQYMQLGGNVFFTIFQPSKMLDYTDDLQHTWQEGSFLHDMLKTDSTKYLTGA
                     VFIGGLPLITGYHMLDVDTNKTYPAYNHHLFNIQAVYPSVEGSVVYSYDTDYDSTTAQ
                     GIMKGLPVGVEYRGDYNAVLLSFPLYYMDSIQSKIVMQYVLQDVFGELPVLIDEKKPG
                     SLAVNVFPNPAQGSFVIRTTDDFLQKKSLDIYDVTGRQILSVTGMQGNENKIDVSRLQ
                     SGIYFLRITGGNRDYNIPMVIVN"
     gene            complement(4436..5464)
                     /locus_tag="PLI16_03510"
     CDS             complement(4436..5464)
                     /locus_tag="PLI16_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83654.1"
                     /translation="MKTHFGSLVDFFSSLFKDVFHTLLILFRIMIPVSIVVKILQMIG
                     FIQVLGQLFYPVMKYTGLPGEMGLVWATGMITNLFGGIIAFLNISGTMELTVAQITTI
                     SVMMLVAHTFPIELTIARKTGVRLISMFFIRLIFAYLFGFIIFHLYALLGLNTEIAQI
                     AWHPETMPDDSLATWAVSELKNYLIIIAFIFCLMLLLRILKKIGFIDLITKALNPFLK
                     VLGIGKEVSTIALVGLTLGVVYGGALIINEVKSNPAIPKKDIFYAMVLMGLCHSVIED
                     TILMVSLGASFTGILVIRLLLSMLVLFVLVKLTRNASDKFMYKVFLRSEKTKFRNKTD
                     QENYSQEL"
     gene            complement(5517..7064)
                     /gene="lepB"
                     /locus_tag="PLI16_03515"
     CDS             complement(5517..7064)
                     /gene="lepB"
                     /locus_tag="PLI16_03515"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOH83655.1"
                     /translation="MSLYAILTIIFFIATLAGLWGLFAKAGQKGWIVLIPFYNFYIWL
                     KIIKKPLWWYVFILVPFINVFTIMLMIVELVKCYNKNGLLAQALSILIPFAYLPYLAF
                     FSKEKYTHPDKQKPYKKSAIREWLDAIVFAVIAATIIRTFFIEAYTIPTSSMEKSLLI
                     GDYLFVDKISYGPRVPMTPLAFPFAHHTLPLTKFTKSYLEWIKLPYYRFPGFTKIKNN
                     DVVVFNYPDGDTVALNMQNQSYYALVRNFGHREVWKDKRKFGNIVARPVDKRENYIKR
                     CIGIPGDSLEIKDQVVYINGQALPEIGIKEYKYLVQTDSSMIPTRIYEKYDITEQVET
                     NGSDLYLMTLTAESAENIKKLNFVRRVEKQVFPKGEWHSYTFPFDSNYAWNEDNYGPL
                     YIPKAGATIPINTKNISLYQRCIEIYENNKVQVSGDKVLINDQPATSYTFKYDYYWMM
                     GDNRHNSADSRFWGFVPVNHIVGRAVFVWLSLEGINCDNQQLNTVRSQQSFFQRLRWN
                     KMFRIIR"
     gene            7327..>7643
                     /locus_tag="PLI16_03520"
     CDS             7327..>7643
                     /locus_tag="PLI16_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83656.1"
                     /translation="MNSKVITSRFWFVVTVILAGAAMRLIPHWPNVTPIAAMALFGGA
                     CLSRKYLAFIIPLAALFLSDLLLGFHSTMPAVYIGFIITVVLGMYMLRNPKLLNVVGA
                     SLSS"
BASE COUNT         2178 a         1486 c         1678 g         2301 t
ORIGIN      
        1 ccgtgtcgga tgccattcgc cgcagccgtg ccggcttgca ggactccaaa aaaccgatag
       61 gatctttttt gtttatgggt accaccggcg taggcaaaac cgaactggcc aaaacgctgg
      121 ccgaatacct tttcagcgac gaaaatgcgc tggtgcgtat cgatatgtct gagtaccagg
      181 aaaggcatac tgtgtcgcgc ctcgtgggtg cgcctccggg ttatgtgggc tacgaagaaa
      241 gcggccagct gaccgaagcc gtgcgccgga aacctttttc ggtggtgttg ctcgacgaaa
      301 ttgaaaaagc ccatccggat gtgtttaaca tcctgcttca ggtgctcgac gatggcaggc
      361 taaccgacaa caaaggccgt accgccgact tcaggaatac catcatcatc atgacttcca
      421 acatcggatc gcacgtcatc caggaaaaat ttgcaggcat caacgatgaa aaccgcgaca
      481 gaatcattga caacacacgg tctgaaatca tcagcctgct gaaacagacg atccgtccgg
      541 aatttctcaa ccgtatcgat gaaattatta tgttcaggcc actgagcagg gaagaaataa
      601 aacagattgt aagtctgcag ttgagcggtg tacagaagat gctcgaacaa aatggtattc
      661 acctcaccgt aaccgaggcc gccctgtcga agattgccat catgggttat gatcccaact
      721 atggcgcgcg ccctgtaaaa agggtgattc agcaggaggt gctcaacaag ctggctacgc
      781 agataatttc aggagctgtg cagaaagaca gcgaggtgga attgcgctta aagaatgatg
      841 attttgagtt tgtaaatttg taaggcgttt ttttgtgaac accagaagca gctaacatcg
      901 cctgccggca ggtatttcat attgcagcgc tttgtcaaat cagtattttt gatttcatat
      961 ttgttttttt gttacctttg gactcgataa caattgaggc agactatttg ttttcattta
     1021 ttccatcctg cggtttttaa atcagcatat ttgttatact tgccagtggt ttgccggata
     1081 ttttgatttt aataatttta tcagataaaa aaattctacg aaatgggcaa aatgtactca
     1141 gcaggtaata aaaaggaaat tcaacaaaac aaagcgaaaa agagacagga aaaggaaaaa
     1201 aaacgacttg aaagaaaaga aaacagcagg cagggtggtg gtttagatga tatgattgct
     1261 tatgtggacg aatttggaaa cattacttcc gaacctcagg atttgtcaaa aagagaagaa
     1321 ttaaatatcg acgacatcgc aataaaaact cagcgaaaaa atgatgcaga accgcaaaac
     1381 aaaattcgta cagggattgt tacatttttt aatgagtcaa agggttttgg gtttataaag
     1441 gatattgaca cccaggaaag catttttttc catatgaaag gattactgga gcctgttaaa
     1501 gaaaacaata aggtgatttt tgaagtagaa aaaggagtta aaggcctgaa tgcttttaat
     1561 gtgaaaattg accgttagca ttgaatttgc tatcacctgt caaaataacc ccggtacaag
     1621 attgatcgcc ccaaatgctg aaaactacaa aacaaccatt ttccggttga attatcccga
     1681 attacgcatt ataatattct aacgcgtagc attagaacac atttttcata atatacttat
     1741 ttttggcata ttagctaaac atttttttgc gtaaacgaca aagaaaatgt aatcccaaca
     1801 aagaaaaatt gtcgggaaac attttctaag tcgggataat cccaactaaa gcaatagatt
     1861 gatgaatcat ttggacaagt tcgattacct ctattgcata aattgggatt atcaaaaact
     1921 gcaaagtata ttttggaggg taatcagcat ttaaatttgt aatgccctga tttgatttgc
     1981 ttatttttat tcaatacctc cggttaatct taaggtttgt ttttgatttt caccgatttt
     2041 taaccggtaa cttgaaaata tttaccctaa actgttttat tatttcacaa ggtgttcaga
     2101 aatcatattt ttattttata ataaataaat gaatatctat gaaaaaaata ttattcattt
     2161 tgtttttgtg cttcagtatg catatttgca ccaggagtca acaaattgct ttgccgcaaa
     2221 caaacgcgac aaatgcattt ctttccttgc cggcatccat tgaaaatatt gattcaagtc
     2281 ccataaagaa agcagtccat gattccctga tcggtgatgt gcttacggat ttgaatgccg
     2341 attctataat gagctacatc cagcatctgc aggcatatgg tactcgtttt ttgcttgctc
     2401 ccggccataa agatatttct ctctggctgc aacacaaatt gatctctttc ggttatacca
     2461 atgcagtgct tgattcgttt gaaataactg tagaatggcc agtgggtagc ggtaacatga
     2521 tcacaaacat tcagtataat gttattgcgg aactcagtgg gctgatcagt cctgatgtgc
     2581 aatatgtcat cggggctcat tatgatgata tcctgcaatt tggtggtcac gatccctatc
     2641 ttgtttgtcc cggtgctgac gataatgcct ccggaactgc tgcagttatg gaaatagcaa
     2701 gagtgctgaa agaaaggaat ttttcttcgc cggccacttt aagatttgtt ttttttgatg
     2761 cagaagagtt gggaacattc ggcagccgtc attttgttga ggacattatg gccggtattg
     2821 aaaaaccgga aattatgttc aatctggata tggtcggtca cgaacctgcc gatacggcct
     2881 ggctgcttca ccttaataat tatgtgggta atgaatggat ttgcagtatg ggcgcggaaa
     2941 ttgctgcaga atttacacag ttgggcactt ttaatctttt ttttaatgaa ccgattggtt
     3001 ccgacagcga ccctttcttt cttgcaggct taccagccat atttctgcag gaagatgatt
     3061 ttagccctta ttatcatacg ttggctgatg tcgacagcac tatcaatggc ctttattgca
     3121 gggagatagc ccgattagct tgtgctatgc ttatgaactc atccttagtt cccacactgg
     3181 tggattttga aatttccaat ccgggcgacg gacattcgtt ggttccaaga tggaggccga
     3241 atccggaaaa taacattgcc gggtacaggg tatactttgg aaccttgcca tcaacttacg
     3301 acactgttta tgctactgca gacacttcac ttatcttttc cggtttatcc acagataccc
     3361 tttattatat ttcggtttct gcagtgaata ctgaaggacg cgaagggatt aaaaatgagc
     3421 tgaatgaccg gcctgctatt gtgaacctcg atcagggaat actgattgtc gatgattcgg
     3481 aaggcggttt ttttgatccg ccggattcgg tagtcgatgc ctattatcgt tatttgtgca
     3541 gcgatttcaa tgttaccgaa tatgatgcca cgcaaaatgg cccgttgact cttgccgggc
     3601 ttgggccgta ttcatcggtg gtgtggcata ttaatagagc gaatatcatc cccaagcttc
     3661 gcagttcact cccggaattg agacaatata tgcaattggg aggtaatgtt ttttttacca
     3721 tttttcagcc ctcaaaaatg ttagattata ccgatgactt gcaacatacc tggcaggaag
     3781 ggtctttttt acatgatatg ctgaaaaccg actctacaaa atatttgacc ggtgctgtat
     3841 ttattggcgg gttgcctctt ataaccggtt atcacatgct ggatgtggat acaaataaaa
     3901 catatccggc ctataatcat catttattta atatacaagc tgtgtatccc tcagttgagg
     3961 ggagtgtggt ttattcatac gatactgatt atgacagcac aaccgcccag ggtattatga
     4021 aaggattgcc ggtaggcgtg gaatatcgcg gtgattataa tgcagttttg ttgtcgttcc
     4081 ctttgtatta tatggacagc atacaatcga aaatagtgat gcaatatgtt ctgcaggatg
     4141 tgttcggcga gctccctgtt ttaattgatg aaaaaaagcc tggctcattg gccgtaaatg
     4201 tctttcccaa cccggcacaa ggatcatttg tgataagaac caccgatgat tttttacaga
     4261 aaaagtcttt ggatatctat gatgtaacag gccggcaaat attatctgtt acaggcatgc
     4321 agggaaatga aaacaagata gatgtttcaa ggcttcaaag cggaatatat tttctgagaa
     4381 ttacaggcgg gaatcgtgat tataatattc cgatggtcat tgtcaattga tttgtttaca
     4441 attcttgtga atagttttcc tgatcagttt tatttcggaa tttggttttt tcagacctca
     4501 aaaacacttt atacataaat ttatctgagg catttcgggt tagtttaacc aatacgaaca
     4561 aaaccagcat actcagcaac aggcgtataa caagaattcc tgtaaacgat gctcccagcg
     4621 ataccatcag gatggtgtct tcgatcacgc tgtgacacag tcccatgagc accatagcat
     4681 aaaaaatgtc ttttttggga atggcagggt ttgattttac ttcgttgatt atcagtgcgc
     4741 cgccgtaaac aacgccaagg gtcagcccaa ccagcgcgat agtgcttact tctttgccaa
     4801 tgcccaaaac ttttaaaaaa ggattcaacg cttttgtaat aaggtcgata aatcctattt
     4861 tttttaaaat tctcagcagc agcataagac agaagataaa ggcaatgatt ataaggtagt
     4921 tttttagttc ggaaacagcc catgtggcaa gggaatcgtc gggcatggtt tcgggatgcc
     4981 atgctatctg agctatttcg gtattcaaac ccagcaaggc gtacagatgg aagataataa
     5041 acccaaaaag gtaggcaaaa atcaggcgga taaaaaacat ggaaatcagc cgtacgcccg
     5101 ttttgcgcgc tatggtcagt tcaatgggaa aggtatgggc aacgagcatc attacgctga
     5161 tggttgttat ttgagcaacg gtaagctcca tggtgcctga gatgttcaga aaagcaataa
     5221 tgcctccaaa aagatttgtt atcatccctg tggcccacac caggcccatt tcaccgggca
     5281 aacccgtgta tttcatcacg ggatagaaca gttgtcccaa tacctgtata aatcctatca
     5341 tctgcaggat ttttaccacg atggaaaccg gtatcatgat acggaacagt atcagcagcg
     5401 tgtgaaagac atctttgaac agcgaagaaa aaaaatcaac cagagaacca aaatgggttt
     5461 tcattggcct gctcttttaa tggttgatcg gatcatgctt gcagcaaaag ctggtattac
     5521 ctgattatcc tgaacatctt gttccaacgc agccgctgga agaaactttg ctggctccgg
     5581 acggtgttca gttgttggtt gtcacagttg atgccttcga gagaaagcca cacaaatacc
     5641 gctctgccca cgatgtgatt aacgggaaca aacccccaga atcgcgagtc ggcggagtta
     5701 tgccggttgt cgcccatcat ccagtagtag tcgtatttga atgtgtatga tgtggccggc
     5761 tgatcattaa tgagcacttt gtcgccgctg acttgtactt tattgttttc atatatttcg
     5821 atgcagcgct ggtacaggct gatgtttttt gtgttgatgg gaatggtggc tccggctttc
     5881 gggatatata acggaccata gttgtcttca ttccatgcgt agtttgagtc gaatggaaag
     5941 gtataactgt gccattcgcc tttagggaat acttgttttt ccaccctgcg gacgaagttt
     6001 aatttcttta tattttcggc tgattcagcc gtgagtgtca tcaggtacag gtcggatcca
     6061 ttggtttcta cttgttcggt gatgtcgtat ttttcataga tgcgtgttgg gatcatggac
     6121 gaatcggtct gcaccaggta tttatattct ttgatgccga tttcgggcaa tgcctgtccg
     6181 ttgatataaa ccacctgatc ttttatttca agggaatcgc cgggtatccc tatacagcgt
     6241 ttgatatagt tttcgcgttt atccaccggt cgtgccacga tatttccgaa ttttcttttg
     6301 tctttccaca cttcgcggtg tccgaaattc ctgaccagcg catagtagct ctggttttgc
     6361 atgttcaagg ctacggtgtc accgtcggga taattaaaaa caaccacatc gttatttttt
     6421 attttggtaa atcccggaaa acgatagtag gggagtttta tccattcgag gtacgacttg
     6481 gtgaatttgg tcagcggcag ggtatggtgg gcaaaaggaa acgcaagcgg tgtcatcggc
     6541 accctcggcc cgtagcttat cttgtccaca aagagataat cgcctatcag caacgatttt
     6601 tccatggaag aggtaggaat agtgtaggct tcgataaaaa atgtgcggat gatggtggct
     6661 gctatcactg caaacacaat ggcatcaagc cattcgcgta tggccgactt tttatagggc
     6721 ttttgtttgt cggggtgcgt gtatttttct tttgagaaaa aagccaggta cggcaggtaa
     6781 gcgaaaggta tcagaatgct cagcgcctgc gccagcagac cgtttttgtt atagcatttt
     6841 accagttcca ctatcataag cattatggta aatacattaa tgaagggtac aaggatgaaa
     6901 acataccacc acaatggttt ttttatgatt ttcagccaga tatagaaatt atagaaaggt
     6961 atcaaaacta tccagccttt ttggcccgct ttggcaaaaa gtccccaaag ccccgctaat
     7021 gttgcaataa agaaaattat tgtcaggatg gcgtataaac tcatgttgat tgttgattaa
     7081 tgattaattg tctttttttt agatgattgg gatattactt tgatgcttta tcagaaaatt
     7141 gttacaaaag tcagcaaaaa aaacgaaaaa aaacaaataa aagtatcggg gaatatcgct
     7201 gtatcataaa atattaatgt attttttttg tttataaaca tttttattgt tgataaatgg
     7261 aaataaaaaa agttgagaat taaaaatgtt ttctattttt gcacttagaa attaaaacta
     7321 tttacaatga attcaaaagt tatcacctcg cgtttttggt ttgtagttac tgtgatttta
     7381 gccggagccg ctatgcgtct catcccgcat tggcccaacg taactccgat tgctgcaatg
     7441 gccctgtttg gcggcgcttg tcttagcaga aagtaccttg cttttatcat tccattggct
     7501 gcattgtttt tgagtgacct gttgcttggg tttcacagca ccatgcctgc cgtgtatatt
     7561 ggctttataa tcaccgtggt tttaggtatg tatatgctgc gtaatcccaa actactcaac
     7621 gtggtaggtg cttcattgtc ttc
//
LOCUS       DAOSFP010000078         7460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8553_length_7460_cov_4.190412, whole genome shotgun sequence.
ACCESSION   DAOSFP010000078 DAOSFP010000000
VERSION     DAOSFP010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8553_length_7460_cov_4.190412"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            601..1923
                     /locus_tag="PLI16_03525"
     CDS             601..1923
                     /locus_tag="PLI16_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH83657.1"
                     /translation="MKDTPINHDVVSRLIAESQIPCIGKATIREVKKLINEIEKETGQ
                     KFVRMEMGVPGLPATQIGVDAEIQALKNGCAAIYPEIDGLPELKTEMARFVKLFMDVD
                     VTPENCIPTVGSMQGGYAAFMTVNRMYKEKDTTLMLDPCFPVHKQQHAVIGTKFEAFD
                     VYDYRGDKLKEKIESYLKKGNIASIMFSNPNNPSWICFTDKELKIIADLANQYNVLVL
                     EDLAYFGMDFRKDYSKPGVPPFQPSVAKYTDNYIMFVSGSKVFSYAGQRIGMMVISPK
                     VFNTHAPDLKRYYASDKFGKAMVYGVIYSLSSGTAHSAQYALAAMLKAANDGKFNFVE
                     EVKEYGEKARIMKKMFTDNGFKIVYDKDENDPLADGFYFTFSYPGFTGEQLLEELMYY
                     GISAISLAITGGRTEGMRACVSLVLRHQFPDLEYRLKKFYEHHPVNSD"
     gene            1994..2227
                     /locus_tag="PLI16_03530"
     CDS             1994..2227
                     /locus_tag="PLI16_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH83658.1"
                     /translation="MAKLKGAIEIDIERCKGCEVCTTACPESVIIMNPGVNSKGYHYA
                     MMGNDNCVGCANCAVVCPDGVITVYRVKVTTEV"
     gene            2246..3331
                     /locus_tag="PLI16_03535"
     CDS             2246..3331
                     /locus_tag="PLI16_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOH83659.1"
                     /translation="MMKELRLMKGNDAIAEAMIRAGSDGYFGYPITPQSEVLEYLMAQ
                     RPYETTGMVVLQAESEVAAINMVYGGASCGKKCITSSSSPGISLKQEGISYIAGAELP
                     CLIVNVVRGGPGLGTIQPSQGDYFQATKGGGHGDYRLIVLAPASVQEMSDFVELGFDL
                     AFKYRNPVMILSDGVIGQMMEKVELKEQKSRWTKEEIVKISPWATTGKTPDRERNVIT
                     SLDLDPARQERFVMKLDRKFKEIEEKEVRYETLNCEDAEYLFVAFGSSARICQKAMDI
                     AREKGIKVGILRPITLWPFPKKPLAELAGKIKGILTVEMNLGQMVEDVRLAVNCSVKV
                     EQYGRVGGIIPTPDEVVSVLETKIIGG"
     gene            3344..4111
                     /locus_tag="PLI16_03540"
     CDS             3344..4111
                     /locus_tag="PLI16_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOH83660.1"
                     /translation="MSREQIIKPENLIYKKTSLLTDKVMHYCPGCGHGTVHRMVMEVV
                     DEMGIQAETIGVAPVGCSVFAYDYMNIDMQEAAHGRAPALATAIKRLMPEKYVFTYQG
                     DGDLAAIGTAETIHACNRGENFVIIFINNGIYGMTGGQMAPTTLPGMKASTCPFGRDT
                     QIMGNPLKITELVATLPGTCYVTRHSVHTPANVRKTKRAIRKGFELSMAKKGLSFIEV
                     VSNCNSGWKMTPVQANKWMEENMFPFYPLGDIKVPAE"
     gene            4159..4710
                     /locus_tag="PLI16_03545"
     CDS             4159..4710
                     /locus_tag="PLI16_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOH83661.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGVMQNKEVSWMPSYGPEMR
                     GGTANVTVIVSDERVSSPILTVFDTAIILNQQSMDKFENSVKPGGLLIYDGNGITRHP
                     ERKDINIYRVDAAEEASKRGMNKVFNMMVLGAYLKMKPIIQPEFIRKGLEKSLPERHH
                     KLIPDNEKAIEIGKEIIREVQKV"
     gene            4797..5741
                     /locus_tag="PLI16_03550"
     CDS             4797..5741
                     /locus_tag="PLI16_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOH83662.1"
                     /translation="MILDKKAIETAAGRIQPFINRTPVLTSQSLNGMFETELFFKCEN
                     FQEAGVFKSRGACNAVFSLSSLSTNKGVATHSSGNHAQALARAAKLRNMKAYIVMPEN
                     SSKVKIDAVKAYGGLITFCEPTLEARESTLKEVIDRTGAVEIHPYDNYQVIAGQGTAT
                     KELLEEIPGLDYIVCPVGGGGLLSGTLLATKIFSPSTQVIAAEPEQANDAFLSFKSKS
                     FVPSVNPDTIADGLRTSLGKLTFPIIMQYLYDIFTVSEQDIIDSMKLIWERMKIVVEP
                     SSAVTLAVVKNNAGLFRKKKTGIILSGGNVDLTRLPWM"
     gene            complement(5755..6348)
                     /locus_tag="PLI16_03555"
     CDS             complement(5755..6348)
                     /locus_tag="PLI16_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83663.1"
                     /translation="MKRLFILAAILLTIGIVPRSLAQKHEVKSPWFKNGSVLTYHVVN
                     GAKEYNYIVSDLDLSKDISFRWKMTEPVNYNGSIKIFDAALDTAMKMVNYFENGSALD
                     MNNKTTAMFSRKAYKMLIGQQPVSVNIDNAQETVGFERYENYHITVDGVQQQVEVMVA
                     STATGKKFWILDNPKFPLIIKMELAFTIDLRTVNTAD"
     gene            complement(6431..>7460)
                     /gene="glpK"
                     /locus_tag="PLI16_03560"
     CDS             complement(6431..>7460)
                     /gene="glpK"
                     /locus_tag="PLI16_03560"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOH83664.1"
                     /translation="AEQGILLFGTIDTYLIWKLSGGKMHITDYSNASRTMLFNIRQLC
                     WDKKLCALFGIPDSMLPQVVDSSGVHGHTQASLFDGAEILIAGIAGDQQAALFGQTCF
                     EEGMIKNTYGTGCFLLMNTGGKPVQSHNGLLTTIAWSIDRQVEYAIEGSVFMAGALIK
                     WLRDNLQMLGSAAESEDFALQALDNGGVYFVPAFSGLGAPYWDMDAKGIITGLTLSSE
                     KKHLIRAALESLAFQTRDVVNAMQLDSGISLKVMNVDGGASSNNFLMQFQANILGVPV
                     IRPFNIESTAMGAAFLAGLAVGLWTREELKLLRKPDKCFAPTMDASERSLLYDAWQKA
                     VAQARNRN"
BASE COUNT         2188 a         1595 c         1632 g         2045 t
ORIGIN      
        1 tgttttatca gagttccgca gtcgttgtgc ttgtgtttgc cttgtagatg taattcttca
       61 caaattcttt caagcctcgt gagtgtttca aaaaaatcgt ttaactcggg tttggtcttg
      121 aagtttttca gcttctctaa acaatcaaaa aattctttgt caaatgccat gatgagatat
      181 tttaatgtta taagtataat ttgcaataat ttgtgaaaaa tacaagttac tgcaataatt
      241 tattaatact cttgccaatc gcattgaatt atgcttatct aaagctatat caattaaaaa
      301 agtattacct tatttatttt cagcacaaat gtaaaactaa gatttgaaaa aaacaaataa
      361 aatttggaaa attattgata aaaagtattt tttcagggac aaaataaaac cacatatttt
      421 tcgttattgc attcaatctc aattatttct gtttacatag ttgattgaat atcaaatttt
      481 tatattttcc ttgctttaat atggcttttt tctcaaattt attttgaagt aaactttgtt
      541 ttttaacaaa ataaatcggt tatttgcaga atagaaaact attaataaac tcaagttaca
      601 atgaaagata cccctatcaa tcacgatgtt gtaagccggc tgattgctga aagccaaatt
      661 ccctgcattg gcaaagcaac catccgcgaa gtgaagaagt taatcaacga aattgaaaaa
      721 gaaacaggcc agaaatttgt ccgcatggaa atgggtgttc ctggtttacc ggccactcaa
      781 attggcgtgg atgctgaaat acaggcattg aaaaatggct gtgccgctat ctaccccgaa
      841 attgacggtt tgccggaatt gaaaaccgag atggcccgtt ttgttaaact ctttatggat
      901 gtggatgtaa cgcctgaaaa ctgtatcccg accgtgggct ctatgcaggg aggttatgct
      961 gccttcatga cagtcaaccg catgtacaaa gaaaaagata ccacgctgat gctggacccc
     1021 tgctttcctg ttcataaaca gcaacatgcc gttatcggca ccaaattcga agcgtttgat
     1081 gtttacgatt atcgtggcga taaactaaaa gaaaaaatag aatcctatct gaagaaaggc
     1141 aatatagcct ccatcatgtt ttctaatccc aacaaccctt catggatttg ttttaccgat
     1201 aaagaattaa aaatcattgc cgaccttgcc aaccagtata atgtattggt tctcgaggat
     1261 ctggcttatt tcggcatgga tttccgtaag gattattcca agcctggtgt accgccattc
     1321 cagccgtcgg tggcaaaata taccgataat tacatcatgt ttgtttccgg ttcaaaggtg
     1381 ttcagttatg ccggacagcg tatcggcatg atggttattt ctcccaaagt gtttaatacc
     1441 catgcccccg acctgaaacg ctattacgcc tccgacaagt ttggcaaggc tatggtgtat
     1501 ggagtcatct attccttgag ttccggaaca gcccacagtg cccaatatgc cctcgccgcc
     1561 atgctgaaag cagctaatga tggtaaattc aattttgtgg aagaagtaaa agagtatggc
     1621 gaaaaagcca gaatcatgaa gaaaatgttt accgacaatg gtttcaaaat tgtgtacgac
     1681 aaagacgaaa atgatcctct ggctgatggt ttttatttca cattttccta tccgggtttt
     1741 accggcgaac aactgctcga agaactgatg tattatggaa taagtgccat atcattagcc
     1801 atcaccggtg gccgcaccga aggcatgcgt gcctgcgttt cactggtgct gcgtcatcag
     1861 ttccccgatc tcgaataccg tctgaaaaaa ttttatgaac accatccggt aaattctgat
     1921 taataattgg taatttggcg gacagtttta atcctgaaaa taattaataa cattttataa
     1981 tatacttaaa aacatggcaa aattaaaagg agcaatagaa attgatattg aacgctgcaa
     2041 aggttgcgaa gtatgcacca cggcgtgccc cgaatcggtg attattatga atcccggggt
     2101 taattccaaa ggataccact atgcaatgat gggaaacgat aattgtgtag gctgtgcaaa
     2161 ctgcgccgtt gtatgtcccg acggcgtaat tactgtgtac agggtaaagg tcacaactga
     2221 agtttaataa ttttaaaatt aaaacataat gaaagaacta agattaatga aaggtaatga
     2281 tgcgatagca gaagctatga tacgtgctgg ctctgacggc tatttcggct atcccattac
     2341 tccacagtcg gaagtattag aatatctcat ggcccaaagg ccttatgaaa ctaccggtat
     2401 ggtggtgctt caggccgaaa gcgaagtggc tgccatcaat atggtttatg gaggcgcttc
     2461 gtgcggaaaa aaatgcatta cctcatcgtc aagccccggc atcagcctca aacaggaagg
     2521 tatttcttat attgccggag ccgaattgcc ctgtctcatc gtgaatgtcg tcagaggagg
     2581 ccccggatta ggaaccattc agccttcaca gggcgactat tttcaggcta ccaaaggcgg
     2641 cggacatggc gattaccgtt tgatcgtcct ggcaccggcc tcagtgcagg aaatgtctga
     2701 ttttgtggaa ttaggttttg acctggcttt caaataccgt aacccggtaa tgatactctc
     2761 tgacggggtt attggtcaga tgatggaaaa agtagaactt aaggaacaaa aaagccgctg
     2821 gaccaaagag gaaatcgtaa aaatatctcc ttgggcaact actggaaaaa cccctgaccg
     2881 cgaaaggaat gtgatcactt ctctcgacct cgatcctgcc cgtcaggaaa ggtttgtcat
     2941 gaaattggac cgcaagttca aagagataga agaaaaagaa gtccgctacg aaacattaaa
     3001 ctgcgaggat gccgaatatc tttttgtggc tttcggatcc agcgcacgta tctgccagaa
     3061 agctatggat atagctcgtg aaaaaggcat taaagtaggc attctccgcc cgattacctt
     3121 gtggcctttc cctaaaaaac ctctggctga actggccggc aaaataaaag gcatactcac
     3181 cgtggagatg aaccttggtc aaatggtgga agatgtccgt cttgccgtaa actgcagcgt
     3241 gaaggtggaa caatacggac gcgtgggagg aattattcct actcccgacg aagtagtatc
     3301 agttttagaa actaaaataa ttggaggtta acccatggca acaatttcaa gagaacaaat
     3361 cataaaaccc gaaaacctga tttataaaaa aacaagcctg cttaccgaca aggtcatgca
     3421 ctattgtccg ggttgcggtc atggtacagt gcaccgtatg gtaatggaag tggttgacga
     3481 gatgggtata caagctgaaa ccattggggt ggccccggtg ggttgctcag tatttgccta
     3541 cgactacatg aacattgata tgcaggaagc tgctcacggc cgcgctcctg ctttggctac
     3601 agccatcaaa agactcatgc ccgaaaaata tgtcttcact tatcagggcg atggcgacct
     3661 ggctgccatc ggtacagccg aaactattca tgcctgtaac cgcggcgaaa attttgtgat
     3721 aatattcatc aataatggta tctatggaat gaccggcgga caaatggctc ccaccacact
     3781 gcccggcatg aaagcatcta cctgcccgtt tggacgagat acacaaatca tgggcaaccc
     3841 attgaaaatt accgaattgg ttgcaactct ccctggtact tgctatgtaa cacgtcattc
     3901 tgttcatacc cccgccaatg tacgcaaaac caaaagagcc atcagaaaag gatttgaact
     3961 gtcgatggcc aaaaaaggac tatcattcat tgaagtggta tctaactgta actcaggatg
     4021 gaaaatgact ccggtacagg ccaataagtg gatggaagaa aatatgttcc ccttctatcc
     4081 tttgggagac attaaagttc ctgcagaata ataatcggta aaacgttact agtttgaaat
     4141 aatataaaat attgaaaaat gactgaagaa attataatag ccggctttgg aggacaaggg
     4201 gttttatcca tgggtaaaat tcttgcctat tccggtgtca tgcaaaataa agaagttagc
     4261 tggatgcctt cttatggccc cgaaatgcgc gggggtacag caaatgtgac agttatcgta
     4321 agcgacgaac gcgtcagttc cccgatactt acggtgttcg atactgccat aattctgaat
     4381 cagcaatcaa tggacaagtt cgaaaactca gtaaaaccgg gcggcttgct tatatacgat
     4441 ggcaacggca ttaccaggca tcctgaacgc aaagacatca acatctaccg cgtagatgct
     4501 gctgaagaag cctcaaaacg gggaatgaat aaagtgttca atatgatggt gctcggcgct
     4561 tatctgaaaa tgaaacccat tattcagccc gaatttatcc gtaaaggcct cgaaaaatcc
     4621 ctccccgaac gtcatcacaa gctgataccg gataatgaaa aggccattga aattggtaaa
     4681 gagattatca gggaagtaca aaaagtgtaa ttccggccgt tttttggttt aaaatttttt
     4741 ttgaaaaggc ttgcgacttt ttgtttcagg cctttttcaa tttttatatg cataggatga
     4801 ttctcgataa aaaagctatt gaaacagccg ccggccgcat acagccattc ataaaccgga
     4861 caccggtgct gacatcacaa agcctgaatg gcatgtttga aacggagttg ttttttaaat
     4921 gcgaaaattt tcaggaagcc ggcgttttta aatcacgcgg tgcatgcaat gccgtttttt
     4981 cgctcagtag cctgtcaacg aacaagggcg tggctacaca ttcctccgga aaccatgccc
     5041 aggccctggc cagggcggca aaactccgaa atatgaaagc gtatattgtt atgcccgaaa
     5101 actcttcaaa agtaaaaatt gatgcggtaa aagcttatgg cggcctgatt actttttgtg
     5161 aaccaacgct ggaggcccgc gaatccactc tgaaagaagt aattgacagg acaggagctg
     5221 tggaaataca cccttacgac aattaccagg tgattgcagg acagggcacg gcaaccaaag
     5281 aattgcttga agaaatcccc ggcctcgatt atatcgtttg tccggtagga gggggaggtt
     5341 tactcagcgg gacactgctg gcaacaaaaa ttttttcacc ctcaacccaa gtcattgctg
     5401 ccgagcctga acaggccaac gatgcttttc tgtcgttcaa atcaaaaagt tttgtgcctt
     5461 cagtaaatcc cgatactatt gctgatggac tcaggacatc tctgggcaaa ttgacctttc
     5521 cgattattat gcaatacctg tatgatattt tcacagtgtc ggaacaggac attatcgact
     5581 ccatgaaact tatctgggaa cgtatgaaga ttgtggttga accttcgtcg gcagtaacac
     5641 tggctgttgt aaaaaacaat gccggtcttt tcagaaagaa aaaaaccggc atcatactct
     5701 ccggaggcaa tgtggatctg acccgattgc cctggatgta aatcctggta cgtcctaatc
     5761 ggcagtattt accgtccgca gatcaatagt aaaggcgagt tccattttta taatcaatgg
     5821 aaatttggga ttgtcaagaa tccagaactt ttttcctgtc gctgtcgaag ctaccattac
     5881 ttccacctgc tgttgtacac catcaactgt gatgtggtag ttttcataac gttcaaaacc
     5941 aactgtttcc tgtgcattgt caatgttgac actaaccggc tgctggccta tgagcatttt
     6001 atacgcttta cggctgaaca tagcagtggt cttattgttc atgtctaagg cagatccgtt
     6061 ttcaaaataa tttaccatct tcatggcagt gtccaatgcg gcatcaaaaa tcttaatact
     6121 gccgttataa ttaacgggtt ctgtcatctt ccaacggaag gatatgtctt ttgacaggtc
     6181 aaggtcactg acaatataat tgtattcttt ggcgccattt accacatgat aggtaagcac
     6241 cgaaccgttt ttaaaccacg ggcttttaac ttcatgtttt tgtgcaaggc tacggggaac
     6301 tatcccgatt gtaaggagta tagcggctaa aatgaatagt cttttcatgg ttgaaatttt
     6361 tatgattaat aaacaaaggt acaatttttc tgttgccttt gtattgtttt tttaaaaatt
     6421 gtttttactg tcagtttcta tttcttgcct gtgcaacggc tttctgccag gcatcataca
     6481 gcaggcttct ttcgctggca tccattgtgg gtgcgaaaca tttgtcgggt tttcgcaaga
     6541 gtttcagttc ttccctggtc caaagcccga cagccaaacc ggccaggaaa gcagctccca
     6601 tggctgtgga ttcgatatta aacggtcgta tcaccggcac accgagaata ttggcctgaa
     6661 attgcatcaa aaaattattt gacgaggcgc cgccgtccac attcatcact ttcaggctga
     6721 tgcccgaatc gagctgcatg gcgtttacca catcgcgggt ttggaaagcc agcgattcaa
     6781 gggctgctcg gataaggtgt tttttttccg aggacagggt gagtcccgta atgatacctt
     6841 tggcatccat gtcccaataa ggtgcgccaa gtcctgaaaa ggcagggaca aaatatacgc
     6901 ctccattgtc aagtgcctga agggcaaagt cttccgattc ggctgccgaa ccaagcattt
     6961 gcaagttgtc gcgcagccat tttatgagtg caccggccat aaaaacactg ccctctatgg
     7021 cgtattctac ctggcggtcg atgctccatg caatggttgt cagcaggcca ttgtgcgact
     7081 gcacgggttt gccaccggtg ttcatcagca aaaaacatcc cgtgccatag gtgtttttta
     7141 tcatgccttc ttcaaaacag gtttgtccga acagtgcagc ctgctggtcg ccggcaatgc
     7201 ctgctatcag aatctctgcg ccgtcaaaga ggctggcctg tgtatgtccg tgaacacccg
     7261 aagaatccac cacttgcgga agcatactat cggggatgcc gaacagggca caaagttttt
     7321 tatcccagca cagttgcctg atattgaaca acatagtgcg tgaagcattg gaataatcag
     7381 tgatgtgcat tttgccaccg cttagcttcc atatcaggta ggtatctatg gtgccaaaaa
     7441 gcaaaatgcc ctgctcagcc
//
LOCUS       DAOSFP010000079         7438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8603_length_7438_cov_4.059867, whole genome shotgun sequence.
ACCESSION   DAOSFP010000079 DAOSFP010000000
VERSION     DAOSFP010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8603_length_7438_cov_4.059867"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PLI16_03565"
     CDS             <1..1041
                     /locus_tag="PLI16_03565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83665.1"
                     /translation="TSRPPVVSTLYDIQRLLTASRRLHKKYGFALVHCRSYIAAFAGL
                     YLKKQQGVRFVFDMRGFWADERVEGGIWNIKNPVYRVIYKYFKRKEREYLLRADHVIT
                     LTEKAKQLIQTHFGLHNKTPEISVTPCCADTQFFSENNIDHAEKVALARQLKITHNDL
                     IITYSGSLGTWYMLDEMLDFFKQLSAVFPAAKFLIITHDNPEKIFTKAKIKNIDTEKI
                     LIHRAFYKQMPLALSLTKIALFFILPVYSKQASSPTKMGEIMSMGIPFITNSGVGDVD
                     AFVKRTGTGLLVKDFTDNSYKETTAKIPVLLNADAAKIRRAALEEYSLDYGISVYYKV
                     YERLTATPNPQS"
     gene            1041..3485
                     /locus_tag="PLI16_03570"
     CDS             1041..3485
                     /locus_tag="PLI16_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOH83666.1"
                     /translation="MHSCDKNENKTGFDQIRQLVSGYCLSEPGKQQLGRIAFSDNYHQ
                     ILHELDLSNEMKTVLTTDDPFPSDDYYDLSDELSRLITPGTFIDIEEMNQLRLSYITI
                     QEIHNYIKQRQQKYPFLFQLTANTVIHDDLICNINRILNEKGEIRDDASEELKAIRQE
                     MTAVQSQIGKRTRQILMSLKKDDIIHPDTEMTVRNGRCVIPMAAADKRKIKGFVHDES
                     ATGQTVFIEPAEIFEMNNQLRELENAERKAIIKILILFTDYIRPFIGELIQEYEFLGR
                     IDCIRAKSLFAIDTNCIKPVLLPTPGINWLRAVNPLLYLHLKEKNKTVVPYDISLHSD
                     QRIVVISGPNAGGKSVCLKTIGLLQYMIQCGFLIPVNEGSEAGIFSDIFIEIGDEQSI
                     ENDLSTYSSHLLNLKHFIENANERTIFLIDEFGSGTEPVLGGAIAQATLEALNEKKSF
                     GAVTTHYSNIKEFAAKAEGVINASMLFDTEKLQPLFKLITGHAGSSYALEIADSIGFP
                     ANITERATNIAGKEHISFEKQVKNFQADKEELLRQQEKVRMADDFFSEMIDKYQKLLS
                     ELETQKKTIITEARQQAALLLQNANKTIENTIRQIKESGAEKEKTKLVREELQELKEK
                     IIADKKPEEVTTKPAASKKQKPTMAAIKAEKKITIPQKGSYVCLPGQKNIGEVLEVNN
                     GQARVLFDNIKITLPLEQLEEVESARYKDFIKKKTRTNYPDISMQLTEKVAGFKTTLD
                     VRGKRAEEITEIIEKYIDEALLLKVYNVRILHGKGSGVLRAIIHRLLSGHPNVVAFEP
                     EHVEHGGDGITVVSLQ"
     gene            3526..3936
                     /gene="trxA"
                     /locus_tag="PLI16_03575"
     CDS             3526..3936
                     /gene="trxA"
                     /locus_tag="PLI16_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH83667.1"
                     /translation="MKNLTLILLLAFIFAIPTNISGNISTKKQNSTNGVLILTDKNFD
                     KTIKKGVVLVDFWATWCGPCRILSPIVEEVASEIGKKAVIGKMDTDQNNITSLKYNIR
                     YLPTIIIFKDGVPMQRFVGMQTKETLINAINQLQ"
     gene            complement(5096..5950)
                     /locus_tag="PLI16_03580"
     CDS             complement(5096..5950)
                     /locus_tag="PLI16_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOH83668.1"
                     /translation="MSPPIALLIGIALSIFGIKHENIHKYTSKILQASIVLMGFGMSL
                     SAVISASKTGFIETTISVTTVMILGVLLGKLLKVDKNTSLLIASGTAICGGSAIAAVA
                     PILKSKSYQTSFALIVVFVLNAVALFLFPFIGNKLGLSQEMFGNWAAIAIHDTSSVVG
                     AGAVYGEKALQVATTVKLIRALWIIPLSLVIAFTNKNDEKKSIKFPWFILLFVLAIVF
                     ANLFPGFLESYEHFNWSGKRGMVIALFLIGSNITLSEIKKTGARSFVFGIALWLLISI
                     GSLFFLTR"
     gene            complement(6129..7016)
                     /locus_tag="PLI16_03585"
     CDS             complement(6129..7016)
                     /locus_tag="PLI16_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOH83669.1"
                     /translation="MDYRDTVFISVAENLSFSKAAHNLNISQPAVTRHIKELEERYKT
                     NLIERKGNKIYLTKAGENVYNAFKEIAKQYRDLDFEIGQLHSSISGEFKIGASSTISQ
                     YVIPKVIASFHKRYPKIQIYLMNGNSFEMEKLLLDNQVDIALVENLSSQSGIRYLDFL
                     DDELIVITGKNSVYAKRETITKDDLMQIPIVLREHGSGTLEVIKQMLSRQNIKFENLN
                     TLIHLGSTESIKNFLQDFDGLAIVSEKAVQNELYMKTLVKLKVSGFTIPRKFRLAYKQ
                     GHKSQQVELFENFLLQYNI"
     gene            7161..>7438
                     /locus_tag="PLI16_03590"
     CDS             7161..>7438
                     /locus_tag="PLI16_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOH83670.1"
                     /translation="MTLTFFKRISEFSSLVKIAHTVFSLPFAVIGFFLAWQQQGASFD
                     VKTFLLMLLCVFFARNAAMSFNRIADRHIDKKNPRTQHRDLPSGKMNTA"
BASE COUNT         2374 a         1618 c         1460 g         1986 t
ORIGIN      
        1 acttcgcgcc caccggtagt ttccactctg tacgacattc agcggttgct gacagcctcc
       61 cgccgcctac ataaaaaata tggttttgcg ctggtgcatt gtcgcagtta tattgcagca
      121 tttgccggat tatacctgaa aaaacaacag ggcgtacgtt ttgtttttga tatgagaggt
      181 ttctgggcag acgaacgggt ggaaggcggc atctggaaca tcaaaaaccc cgtttaccgg
      241 gtgatataca agtattttaa acgcaaagaa cgcgaatacc tgctccgggc cgaccatgta
      301 atcacgctta ctgaaaaggc aaaacaattg atacaaacgc attttgggct gcacaacaag
      361 actcccgaaa tcagcgtaac tccctgttgt gccgacacac aatttttttc tgaaaacaat
      421 attgaccacg cagaaaaagt ggcactggcc cgtcaattga aaattacaca caacgacctg
      481 ataatcacct attcgggctc gctgggaacc tggtatatgc tcgacgaaat gctggatttt
      541 ttcaaacaat tatctgcagt ttttcctgct gcaaaatttc tgattatcac tcacgacaat
      601 ccggaaaaaa ttttcactaa agccaaaata aaaaatattg atacagaaaa aatattgatt
      661 caccgggctt tttacaagca aatgcctctg gcactttccc tgacaaaaat tgcgctgttt
      721 ttcatacttc ctgtgtattc caaacaagcc tcctccccga ccaaaatggg cgaaatcatg
      781 agcatgggta tcccgtttat cacgaacagc ggtgtaggcg acgttgatgc ctttgtgaaa
      841 agaaccggga cagggctgct tgtcaaagat ttcacagaca attcgtataa agaaaccact
      901 gcaaaaattc cggtgctgct gaatgccgac gccgcaaaaa tcaggcgtgc ggccctggaa
      961 gaatactccc tggactacgg catcagcgtt tattacaagg tatatgaacg ccttaccgca
     1021 acacctaatc cccagtcata atgcactctt gtgataaaaa tgaaaacaaa accggtttcg
     1081 accaaatccg gcaacttgtg tcgggttatt gcctgagtga acccggaaag caacaactgg
     1141 gacgaattgc cttttcggat aactatcatc aaattctgca tgaactggac cttagcaacg
     1201 aaatgaaaac ggtgttgaca accgacgacc cgtttccctc agatgactac tacgacctca
     1261 gcgatgaact ttccaggcta atcactccgg ggacattcat cgacattgag gaaatgaacc
     1321 agttacgact gtcctacatc accattcagg aaattcataa ttacatcaaa caaagacagc
     1381 aaaagtatcc ttttctgttc cagctcaccg ccaataccgt tattcacgac gaccttatct
     1441 gcaacatcaa caggatactc aacgaaaaag gcgaaatcag ggatgatgct tcagaagagt
     1501 taaaagccat tcgtcaggag atgaccgcgg tacaatcaca gatcgggaaa cgtacccggc
     1561 aaatattgat gtcgctaaaa aaagacgata tcatacatcc cgacactgaa atgacggtgc
     1621 gcaacggacg ctgcgtgata cccatggctg cagccgataa gcgcaagata aaaggttttg
     1681 ttcatgatga atcagcaaca ggtcaaacgg tgtttatcga acctgccgaa atatttgaaa
     1741 tgaacaacca attgcgtgaa ctcgaaaatg cggaaagaaa agcgataata aaaatactca
     1801 tactgtttac cgattatatc aggcccttca tcggagagct gatacaggaa tatgaatttc
     1861 tgggcaggat agactgtatc cgcgccaaat ccctgtttgc catcgacacc aattgtatta
     1921 agccggtttt attgccaacg ccgggcatca actggctgag agccgttaac ccactgttgt
     1981 atctgcattt aaaagaaaaa aataaaaccg ttgttccata cgacatttcg ctgcattccg
     2041 accaacggat agtggtaatt tcggggccca acgccggagg aaaatcggta tgcctgaaga
     2101 ccatcggact gctgcaatac atgatacaat gcggattcct gataccggta aatgaaggct
     2161 cggaagccgg tattttttcg gatattttta ttgaaatcgg cgacgaacaa tccatcgaaa
     2221 acgacctcag cacctacagt tcacacctgc taaacctgaa acattttatt gaaaatgcca
     2281 atgagcgtac catctttttg attgatgaat tcggcagtgg caccgagccg gtacttggcg
     2341 gagccatagc ccaagccacc ctggaagcgc ttaatgaaaa aaaatctttt ggcgctgtaa
     2401 ctacgcacta ttcaaatatt aaagagtttg cggcaaaagc cgaaggtgtt attaacgcat
     2461 ccatgctgtt cgacacggaa aaactccaac ccttgttcaa gcttatcact ggccatgcgg
     2521 gaagttctta tgccctcgaa attgccgaca gtatcgggtt tccggcaaac atcaccgaaa
     2581 gagccaccaa cattgccgga aaagaacata tctccttcga aaaacaggta aagaattttc
     2641 aagccgacaa agaagaactg ttgcgccagc aggaaaaagt gagaatggcc gacgattttt
     2701 tttcggaaat gattgacaaa taccaaaaac ttctgtcgga attagaaacg cagaaaaaaa
     2761 caattataac cgaggcaaga caacaagctg ccttactgct gcaaaacgcc aataaaacca
     2821 tcgaaaacac catacggcag ataaaagaat cgggggcaga aaaagaaaaa acaaaactag
     2881 taagagaaga acttcaggaa ctgaaagaaa aaattattgc agataaaaaa cctgaagaag
     2941 ttacaacaaa accggcggct tcaaaaaaac aaaagcctac aatggccgcc atcaaagccg
     3001 aaaagaaaat caccataccc caaaaaggca gttatgtctg cctgccagga caaaaaaata
     3061 tcggggaggt gctggaagtg aataatggcc aggcgcgggt gctgtttgac aatatcaaaa
     3121 tcaccttacc gttagaacaa cttgaagagg tagaatcagc cagatacaaa gactttataa
     3181 aaaagaaaac ccggacaaac taccctgaca tcagcatgca actgactgaa aaagtagccg
     3241 gatttaaaac caccctcgat gtgcgtggca agagagccga agaaattacc gaaatcatcg
     3301 aaaaatatat tgacgaagcc ctgttgctta aagtttacaa tgtgaggata cttcatggca
     3361 agggatcggg cgtcctgcgc gccatcatcc accgactatt gtcggggcac cccaatgtgg
     3421 tggcttttga gcctgagcat gtagaacatg gcggcgatgg cattaccgtg gtcagcttac
     3481 aataagtttg gactgattat tgataaaaac cttaaaaatt attttatgaa aaatctaacc
     3541 ttaatactgt tactggcttt tatttttgcc atacccacca atatttcggg aaacatcagc
     3601 accaaaaaac aaaacagcac caatggcgtg cttatattaa cggacaagaa ctttgacaag
     3661 acgataaaaa agggagttgt tctggttgat ttttgggcaa cctggtgtgg tccatgcagg
     3721 atactttctc ctattgttga agaagtggct tcagaaattg gcaaaaaagc cgtcattggc
     3781 aaaatggata ccgatcaaaa caacattact tcgctgaagt acaacatccg gtatttgccc
     3841 accatcatta tttttaaaga tggtgtgccc atgcagcgtt ttgtaggaat gcaaaccaaa
     3901 gaaacgctga taaatgctat aaaccagttg caataaattt tttgacaagg ttttcaaccc
     3961 ttataaccag ctttagcatg tttggctggt ttactaactt tttttataaa aaaaattaca
     4021 tgaacaggta tttgccctta ataccagttg acgaagctta gcagtggaag tgggcgtatg
     4081 actgcgcgtg gccatgctct gaagaagatc tttctcccac tgaaaacata atgctttttt
     4141 attgtacttt tagcttgacc caaaagtacc caaaaggtca agaaaatata aggcgaaccc
     4201 tgcctggccg gcaggcaggc ttcacacagg ccactccctg acaccgccat attttctggc
     4261 cagcgcactg ttgctgctgt gcagcaattc gaaacgctcc aacaaacatt ttgcttaata
     4321 gcaaacgttt atcatttttt atagtcttta aaaatgtttg cacatagcac aaatggctat
     4381 taatagcgcg cgcggtggcc cgtcaaaacg gcgagcctgc gcaagagtga atcatcagaa
     4441 cggtgaaaat cagcaaaggc agcccggcta accgggcttt ttgtctgaaa tggcctgcac
     4501 taatcgcaat ggactttaca cttttttcgt cccaaataat cttgcaaaaa gttcttctcc
     4561 tttgaggcca tttcgggcac tactttgcat gattttcccg ttctgatgag agcgggcgga
     4621 agcataagtt ttgacaagtt tttttggtga cttttttttc aaaaaaagcc acttcaatca
     4681 taaaaaatat tgttcctgta aaaaaccact attgtccgat taaatatatc aaactcgcta
     4741 aaatcattga aaatgttaca tccctaacag gacttaatct atctgactta cttatcattc
     4801 tatagatatt cagtccttac aggactgtcc cgatagggac aatatatttg taaaaacaag
     4861 atataaaatg aaattaagtc ccgtttggaa cgaaataata ttcactggac aatatggata
     4921 aaaaacacag gggaaagtgc attttttgaa caatttttat attacctccc catacaggtt
     4981 gttcttctaa acggacactt tcgcacaagc catcattgaa ttccggctac caacaaggtt
     5041 tttatattaa gagtccgatt aactgtttgc acatagtaca aatgttttgc attgcttatc
     5101 gtgttagaaa gaacagtgaa ccaatagaga tcaaaagcca taaagcaatt ccaaacacaa
     5161 aacttcttgc tcctgtcttt tttatttcac ttaatgtaat attagaacct atcagaaaca
     5221 aagcaatgac catccctcgt ttacctgacc agttaaaatg ctcataactt tccagaaaac
     5281 cgggaaaaag attggcaaaa acaatggcaa gcacgaaaag taaaataaac caggggaatt
     5341 taattgattt tttctcatcg tttttgttcg taaatgcaat aaccaacgat aatggaataa
     5401 tccacaacgc acggataagt tttaccgttg tagcgacctg caaagctttt tcgccataaa
     5461 ccgcccctgc cccaacaacc gaacttgtgt cgtgaatggc aatggccgcc caatttccaa
     5521 acatttcctg gctaaggccc aatttattcc cgataaaagg gaataaaaac aatgctacgg
     5581 catttagtac aaagaccact ataagtgcaa acgacgtttg ataactttta ctctttagaa
     5641 ttggggccac tgcggcgatg gcacttcccc cgcaaatggc cgtgcctgaa gcaataagca
     5701 atgaagtatt tttatcaact tttaaaagtt ttccgagcaa aacaccgagt atcatcacgg
     5761 tggttacaga gattgtcgtt tcaataaaac ccgtttttga tgccgaaatt acagcgctca
     5821 aactcattcc aaaacccatt aaaacaatgg aagcctgaag aattttggat gtgtatttat
     5881 ggatgttttc gtgttttatt ccaaaaattg aaagcgcaat cccaatcaat aaagcgattg
     5941 gcggagacat aaatggcaaa aaacatgtgc aaataataat tgaataaagc cagttgctgt
     6001 tttttaactt tcccttgttg ccacccagcg tgttgaagaa ctttccgttt atcatgccag
     6061 tactttttgc gcacaaaagt attggttcat tataattaaa gcaaatacca aatatttata
     6121 gtcgataact atatgttata ctggagtaag aaattctcaa acaactccac ctgttggctt
     6181 ttatgtcctt gtttgtatgc taaccggaat tttcgtggaa tggtaaatcc tgacactttt
     6241 agtttgacaa gtgttttcat gtagagctcg ttttggacgg ccttttccga tacaatggct
     6301 aatccgtcga aatcttgcaa aaagttttta atcgactcgg tacttcccag atgaatcaac
     6361 gtattgaggt tttcaaattt gatattttgt cgcgatagca tttgctttat cacttccaaa
     6421 gtccccgaac catgttcgcg caacacaatc ggtatctgca tcaaatcgtc tttggtaatg
     6481 gtttcgcgtt tagcataaac actgtttttg ccggtaataa caatcaactc atcatcaaga
     6541 aaatccaggt atcggatgcc tgattgcgac gaaaggtttt caaccaaagc aatatctacc
     6601 tggttatcca acagcaattt ttccatctca aacgagttgc cgttcatcag gtaaatttga
     6661 atttttggat atcgcttatg aaacgatgcg attaccttgg gaataacata ttgtgaaatg
     6721 gttgaactgg cgccaatttt aaattcgccc gaaatgctgc tatgcaattg tcctatttca
     6781 aaatcgagat cacggtattg ttttgcaatt tctttaaaag cattatatac attttcgccg
     6841 gccttggtca ggtaaatttt gttccccttt cgttcaatta ggtttgtttt gtaacgttct
     6901 tccaattctt taatatgtcg tgtaaccgca ggctgactta tattgaggtt gtgggccgct
     6961 tttgaaaaac tgaggttttc agcaactgat ataaaaacgg tatctctgta atccatataa
     7021 aattatatac tttcaaaatt aaacaaaatg gtcaacgagg aattgagggg ttatggcaat
     7081 gtgcttaatg cactgataaa aataggaaaa atttgtattt caacgggctt attgttttct
     7141 ttgctgaagc aaaagaataa atgacgctta cattttttaa acggatttcg gaattttcgt
     7201 cgctggtaaa aattgcccac accgtgtttt cattgccttt tgctgtcatt ggcttttttc
     7261 tggcgtggca gcagcaaggc gcatctttcg atgtaaaaac ttttttgctc atgctgctgt
     7321 gcgtattttt tgcccgtaac gccgccatga gtttcaaccg tattgccgac cggcatatcg
     7381 acaaaaaaaa tccccggacc caacaccgcg acctcccatc ggggaaaatg aacacggc
//
LOCUS       DAOSFP010000080         7411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8651_length_7411_cov_3.312262, whole genome shotgun sequence.
ACCESSION   DAOSFP010000080 DAOSFP010000000
VERSION     DAOSFP010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_7411_cov_3.312262"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLI16_03595"
     CDS             complement(<1..834)
                     /locus_tag="PLI16_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J/K"
                     /protein_id="HOH83671.1"
                     /translation="MANTKVIVGLGSCGVAAGAAKIFDKLALLKKAENLSFELNKTGC
                     IGMCFREPLVEVIDKTGSFLYGNVDENKVIDIVEKHLKNNEPVKEFVVHSNMFATADD
                     YFFEAQVKIALRNCGFINPESIEEYESRDGYNAIKKILNEKMSNEDVIKTMVDSGLRG
                     RGGGGFPTGMKWKFAFNNKADQKYIICNADEGDPGAFMDRSLLEGDPHSVLEGMIIGA
                     YTIQATSGVIYCRAEYPLAIKRLNIALEQARAKGYLGKNIFGVDGFDFDIYIKEGAGA
                     FV"
     gene            complement(855..1373)
                     /gene="nuoE"
                     /locus_tag="PLI16_03600"
     CDS             complement(855..1373)
                     /gene="nuoE"
                     /locus_tag="PLI16_03600"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOH83672.1"
                     /translation="MRIARLPQHKIAEYHSDTDLALLEPVLKKYQNVKGNLIPILQHT
                     QDIFGYIPREAFIKIAETTGLKLSDMYGVATFYAQFRLKPVGKHIIRVCHGTACHVQN
                     SNKITDSLQEALNVKDGDTTEDGLFTLESVACLGCCSLAPVMMIGVETFGKLTGSEAV
                     RIIKEIQIKESN"
     gene            1884..3278
                     /locus_tag="PLI16_03605"
     CDS             1884..3278
                     /locus_tag="PLI16_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOH83673.1"
                     /translation="MKTKYIDLIQQTFDFPQEEFKVEENELYFNGICLTDLIKQYGTP
                     LRISYLPKITSQIQKAKKLFNVAFAKADYQGEYHYAYCTKSSHFQFILHEVLKNDVHL
                     ETSSEFDISIIDSLYKEKKIDKEIYIICNGYKKQGYIEEICRLINSGFVNTIPVLDNK
                     KELEHIGELVKKKCKLGIRIASEEEPRFDFYTSRLGIRYNDIVDFYKANLKNNPKFEL
                     KMLHFFINTGIRDNAYYWSELAKCVNVYCELKKICKDLDSLNIGGGFPVKNSLAFDYD
                     YEYMAEEIVLQIKSICQQNNVVEPNIFTEFGSYTVGESGAIIYSILGQKQQNDRELWD
                     MIDSSFMTTLPDTWAINQRFVLLAVNHWDREYQRVHLGGLTCDSHDFYNAEAHANAVF
                     LPKYEEAETLYIGLFHTGAYQESISGYGGIQHCLTPALKHILLERDEDGEVYPRLFAK
                     EQSHKSMMKILGYM"
     gene            3414..4106
                     /locus_tag="PLI16_03610"
     CDS             3414..4106
                     /locus_tag="PLI16_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83674.1"
                     /translation="MKKNISAFVIIMSVALIFGINSFAQKTFKGIITYTIAYSGNIDP
                     ATAAQQPKTMILSIYENKQKMNLPLGPINIDVITDGDNKTSTTLIDMMGDKKFYKITK
                     EELDKKIAEEPVPAIAYKDETKTIAGYVCKKAEYAISKDGETNTTMVYYTEELGNESL
                     NYGGQFHSLKGVPLEYVITTPDGIITTWTASEVKKGKVKETDFLIPDDYVELSAEEKE
                     QMMKMFSGEGEE"
     gene            4178..6709
                     /locus_tag="PLI16_03615"
     CDS             4178..6709
                     /locus_tag="PLI16_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOH83675.1"
                     /translation="MPRREQTFSKNTFKEEPLAEEPVSSKPKKEPSKKEKTKKVKKEK
                     IDSQRAVGLIETVKSEKFQKVTGLFFLLFAFFLLVAFTSYFLTWKTDDNIFDNSIWRV
                     LGDNSLQSENWMGKIGAVFSFLFIKNSFGIASALFVAWLVILGSKMLFNAQIFPLAKG
                     TVYTLFGLFWISVTLAFVLPNEKNLILSGAFGYQINVWLNSVIGKIGTGILLFFLMVS
                     FLIVEYNLHKLFGKKPKEIAEELPAKDTETESEKTFAKNQFSEAEHETPEDDVEFEVT
                     KKPGPVVEQQPIELVNNTTTKTQKEPGKDEIPFTITPPEEITDTEKVFTPETEEESEE
                     HITIDTPYDPRLELSSYRFPGLDLLKEYDSGNISVQNEELEANKNRILTTLNNYSIQI
                     DKINATIGPTVTLYEIVPSPGIRISKIKNLEDDIALSLSALGIRIIAPMPGKGTIGIE
                     VPNQNPRIVSMRSILASEKFQNNSYDLPIGLGKTISNTSYVADLAKMPHLLMAGATGQ
                     GKSVGLNAIIASLLYKKHPAELKFVLVDPKKVELTLFAKIERHYLAKLPDSEEAIITD
                     VRKVVRTLNSLCILMEMRYDLLKLAGVRNIKEYNTKFIARKLLPTEGHDFMPYIVVIV
                     DEFADLIMTAGKEVEAPITRLAQLARAIGIHLIVATQRPSVNVITGTIKANFPARLAY
                     RVISKIDSRTILDTGGADQLVGRGDMLLSTGNEMVRLQCAFIDSPEVEILTDYIGQQR
                     AFPDAFDLPECPDEESNNDKNLDSSERDEMFDEAARVIVIHQHGSTSLLQRKLKLGYN
                     RAGRIIDQLEAAGIVGPFEGSKAREVRYKDMEALEQFLNTLRNNN"
     gene            6716..7360
                     /locus_tag="PLI16_03620"
     CDS             6716..7360
                     /locus_tag="PLI16_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOH83676.1"
                     /translation="MKKIFVTALLISVAAVINAQNNTQAKTILDGVSQQTKSYSSIDI
                     SFSFTVQNTAKKTKDTKSGFASMKGEKYWSDFAGQEVMCDGKTVWTYLKESNEVQINT
                     NEPDNDQALNPLNLLNDYSKSYTPKFIKEETRGSRIFQILDLTPVKAKSYYKVRLEID
                     KAAKHIVNAIIYDKNGVNTYTYCITKFVTNKTIPESKFTFKASDHPGVEVIDLR"
BASE COUNT         2395 a         1678 c         1394 g         1944 t
ORIGIN      
        1 cacaaatgcg ccggcgcctt ccttgatata aatatcaaaa tcaaagccgt caaccccgaa
       61 aatgtttttt cccaggtatc ctttggcgcg ggcctgctcc aacgcaatat tcagcctttt
      121 tattgccaga ggatattccg cgcggcaata aataacaccc gaggttgcct gtatggtata
      181 agcaccaata atcatccctt ccagaacaga atgcggatca ccttccagca acgaacggtc
      241 cataaaagca ccggggtcgc cttcgtcggc attgcagata atatatttct ggtcagcctt
      301 gttgttaaac gcaaatttcc atttcatgcc ggtaggaaat ccgccgcccc ccctgcctct
      361 aagacctgaa tctaccatag tctttatgac atcttcatta ctcatcttct cattcagtat
      421 ctttttaata gcattgtatc cgtcccgact ttcgtactct tcgatgcttt ccggattgat
      481 aaaaccgcaa ttacgcaggg caatcttcac ctgagcctca aaaaaatagt catcagcggt
      541 agcaaacata ttggaatgaa ccacaaattc ttttacaggt tcattgtttt ttaaatgctt
      601 ttcaacgatg tcaataacct tattttcgtc cacattacca taaagaaaag acccggtttt
      661 atcgataact tccacaagcg gttccctgaa acacattcca atgcaacctg ttttgttcag
      721 ttcaaatgac aggttctcgg ctttcttcaa caacgctaat ttgtcgaata tttttgcggc
      781 accggcagct actccgcaac tccccaggcc cactattact tttgtatttg ccataattat
      841 atcagatttg gctattaatt cgattcctta atttgaattt cttttataat cctgacagct
      901 tcgctcccgg tgagtttgcc aaaagtttct acgcctatca tcatcaccgg ggccagcgag
      961 caacagccca ggcaagccac cgactccagc gtaaaaagtc catcttcggt ggtatcaccg
     1021 tcttttacat ttagtgcttc ctgcaacgaa tcggtaattt tattggaatt ttgcacatgg
     1081 caagcggtac catgacacac cctgatgata tgttttccaa caggtttcag ccgaaattgg
     1141 gcatagaaag tagccacacc atacatatca cttaatttca atccagtcgt ttcagcaatt
     1201 tttataaagg cttctctggg aatatatcca aaaatatcct gtgtatgctg cagtatggga
     1261 atcaaatttc ctttaacatt ttgatatttt ttcaggacag gttccaacaa agcaaggtcg
     1321 gtatctgaat gatattcagc aattttatgc tgagggaggc gtgcgattct catgccgatt
     1381 gatttttaac aaatttaatt atttcttaac aaaatcagaa attttttttt attttttttc
     1441 aggcgaacct gacacacatt acgtgctttt gacaaaacaa agtccacagg gtcatcaaaa
     1501 ttaaaaaaaa gctgacttgt agtaaattaa cgataaactc atggaaaaac ggaagcgaaa
     1561 atcaaaaaac tcatcttata gttaagaaag attcctggca aggtgttaat atgtatctga
     1621 aaaggacctt gcggatctca taaacaaggg gcagtaaaat caattatcac aaaataatgg
     1681 tgtcggtcat acacatacaa actgcgaaaa aattttgaaa acagtgcaca gaacaaggat
     1741 aacttttgcc aatataaata atttttatgt ttttctaaat aaaataattt ttttaatcag
     1801 aattatatac ttttgcacgt tttttgaaat ttaaatcact tacttttata tttttttgtc
     1861 aaaccaacta acttacttta gaaatgaaaa caaaatatat cgaccttatt cagcaaacat
     1921 ttgacttccc ccaggaagaa ttcaaagtag aagaaaatga actttatttt aatgggattt
     1981 gccttactga tttgattaaa cagtacggaa cacccctgcg aatcagctat ttacccaaga
     2041 tcacctcgca aatacaaaag gcaaaaaaac tcttcaatgt ggcttttgcc aaagcagact
     2101 atcagggcga ataccattat gcctattgca ccaaaagctc tcactttcag tttattttgc
     2161 atgaagtact caaaaacgat gtacacctgg agacatcgtc cgagtttgac atcagcatta
     2221 tcgacagcct ttataaagag aaaaaaattg ataaagaaat ctacattatc tgtaacggtt
     2281 acaaaaagca gggatacatt gaagaaatat gccggctgat taacagcgga tttgttaaca
     2341 ccattccggt tcttgacaat aaaaaagaat tagaacacat tggcgaacta gtaaaaaaga
     2401 aatgcaaact cggaattcgt attgcttcgg aagaagaacc ccgttttgat ttttacactt
     2461 cccggcttgg catacgatac aacgacattg ttgattttta taaagccaac ctcaaaaaca
     2521 acccgaaatt cgagttaaag atgttacatt tctttatcaa cacaggaata agggataatg
     2581 cctattactg gagtgagtta gccaaatgtg taaatgttta ttgcgagttg aaaaaaatat
     2641 gtaaggatct cgactcattg aatatcggcg gaggatttcc tgtaaaaaac tcactggcct
     2701 ttgattacga ttatgaatac atggccgaag aaattgtatt gcagataaaa agcatctgcc
     2761 agcaaaacaa tgtcgtcgaa cccaatattt ttaccgagtt tggctcatat actgttggtg
     2821 aaagtggcgc aatcatttat tccatacttg gacaaaagca acaaaatgac cgtgagttgt
     2881 gggatatgat cgacagctct tttatgacta ccctgcccga cacctgggct atcaaccagc
     2941 gctttgtcct gctggccgtt aatcactggg accgggaata tcagcgggta catctggggg
     3001 gacttacctg cgacagccat gatttttaca atgccgaagc ccatgccaac gctgtgttcc
     3061 tgccaaaata cgaagaggct gagacactgt acataggcct ttttcatacc ggagcctacc
     3121 aggaatctat cagcggttat ggcggtattc agcattgcct cacaccagcc ctcaaacata
     3181 tcctgctcga acgcgatgaa gacggcgaag tatatcccag gctttttgcc aaagaacaaa
     3241 gccataaatc catgatgaaa atactgggct atatgtgatt tgcactgtta atttctttat
     3301 tgataacttt tattgacagg cagtatgtac aaatttttta accgatacct ttgtgaaaaa
     3361 ataattggta ttatatttgc aaacacgttg taaatcttaa aaaaaactta accatgaaaa
     3421 aaaacatcag tgcatttgtt atcatcatgt ccgttgcatt aatcttcggc atcaacagtt
     3481 ttgcacaaaa aacatttaaa gggatcatca cttacaccat tgcttattcc ggcaatattg
     3541 accctgccac ggcagcacag cagcccaaaa cgatgatctt gtcaatatac gaaaacaaac
     3601 aaaaaatgaa ccttcccctg ggtcctatta atatcgacgt aattaccgac ggcgacaata
     3661 aaaccagcac taccctgatt gatatgatgg gcgataagaa gttttataaa attacaaaag
     3721 aagaactgga caaaaaaatc gccgaagaac cggtacccgc tattgcttat aaagatgaaa
     3781 caaaaaccat agcgggatat gtctgtaaaa aagccgaata cgctatttcg aaagacggcg
     3841 agaccaacac caccatggtt tactacacag aagaactcgg taacgaaagc ctgaactacg
     3901 gcggacagtt ccattcattg aaaggtgttc ctttggaata tgttattacc actcctgatg
     3961 gcatcattac cacatggaca gccagcgaag ttaaaaaagg caaagtgaaa gaaacagatt
     4021 tccttatccc cgatgattat gttgaactct cagcagaaga aaaagaacaa atgatgaaaa
     4081 tgttttccgg agaaggagaa gaataaaaat aatgaaccgc taaataaaat cctctgaagt
     4141 caaacagggg attttatttt tttaagctat tgagaaaatg ccccgaagag aacaaacttt
     4201 ttcaaaaaat actttcaagg aagaaccgct tgctgaagaa cctgtgagct ctaaaccaaa
     4261 aaaggagccc tcaaaaaaag aaaaaaccaa aaaagttaaa aaagaaaaaa tcgactcaca
     4321 aagggccgtc gggctgattg aaactgtcaa aagtgaaaaa ttccaaaaag taacaggact
     4381 ctttttcctg ctttttgcct tttttctgtt ggtagctttt acttcctact ttcttacctg
     4441 gaaaaccgac gacaatatct ttgataattc aatctggagg gtgcttggcg acaacagcct
     4501 gcaatccgaa aactggatgg gaaaaatagg cgctgttttt tcttttttgt ttataaaaaa
     4561 ctcgtttggc atcgcctctg ccctgtttgt tgcctggctg gtcattcttg gcagcaaaat
     4621 gttgtttaat gcccagatct tcccccttgc aaaaggaacc gtgtacaccc ttttcggatt
     4681 attctggatt tcggtaaccc ttgcttttgt attacccaac gaaaaaaacc tcatcctgag
     4741 cggcgctttt ggttatcaga tcaatgtctg gctaaacagt gtcattggaa aaattggcac
     4801 agggatattg ctttttttcc tgatggtcag cttcctgatt gttgaataca acctccataa
     4861 acttttcggg aaaaaaccca aagaaattgc tgaagaactt ccggccaaag atacagaaac
     4921 tgaatcggag aaaacttttg caaaaaatca attcagtgag gcagaacatg aaacccctga
     4981 agatgatgtg gaatttgaag taaccaagaa gcccggaccc gtcgttgaac agcaacctat
     5041 tgaacttgtt aacaacacca ccacaaaaac tcaaaaagaa cctggcaagg atgaaattcc
     5101 ctttactatt acgcctcccg aagaaataac tgatactgaa aaggttttta ccccggaaac
     5161 agaagaggaa tccgaagaac acattaccat cgataccccg tatgacccca ggcttgagct
     5221 ctcctcatac cgctttccgg gtctcgacct gctgaaagaa tatgattcag gcaatatctc
     5281 ggtgcaaaac gaagaactcg aagccaacaa aaaccgcatc ctcacaaccc tgaacaatta
     5341 ttccatccag attgacaaaa tcaacgctac catcgggcct acggtaacac tctatgaaat
     5401 tgtaccctcg ccaggcatac gcatctcaaa aattaaaaac ctcgaagacg atatcgccct
     5461 gagtctgtcg gccctgggaa tacgcatcat tgcccccatg cccggaaaag gaaccatcgg
     5521 tatcgaagtc cccaaccaaa atccgcgcat tgtctccatg cgctccattc tggcttccga
     5581 aaaatttcag aacaacagct atgatctgcc gataggcctc gggaaaacta tttcaaacac
     5641 cagttatgtg gccgacctgg ccaagatgcc ccacctgctg atggccggcg ccaccgggca
     5701 gggaaaatcg gttggcctca acgccatcat cgcctctttg ttatacaaaa aacaccctgc
     5761 agaacttaaa tttgttttgg ttgaccccaa aaaggtggag ttgacgcttt ttgctaagat
     5821 agaacggcat tatttagcca aacttcccga tagcgaagag gccatcatca ctgatgtgcg
     5881 caaggtggta cgcaccctta actccctctg tattttgatg gaaatgcgtt atgacctctt
     5941 gaaactggcc ggtgtaagga atatcaagga atacaacacc aaattcatcg cccgcaagtt
     6001 actacctact gaaggccacg actttatgcc ttatatagta gtcatcgtgg atgaatttgc
     6061 cgaccttatc atgacggccg ggaaagaagt ggaagccccc atcacgcgcc ttgcacagct
     6121 tgcaagagcc attggcattc acttgatagt ggccacccaa cgtccttcgg tcaatgtgat
     6181 taccggcacc ataaaggcaa acttccctgc ccgcctggcc taccgtgtca tttccaaaat
     6241 cgactcccgt actattctgg ataccggcgg agccgatcaa cttgtaggac ggggcgatat
     6301 gctgctgtca accggaaacg aaatggtacg tctgcaatgt gcctttatcg actcacctga
     6361 agttgaaatc cttactgact atataggtca gcaacgcgcc ttccccgacg ctttcgactt
     6421 accggaatgc cctgacgaag aaagcaacaa cgacaaaaac ctcgactctt cggaacgcga
     6481 cgagatgttt gacgaagccg cgcgtgtcat cgtgatacat cagcatggtt ctacatccct
     6541 gctgcaacgc aaactaaaac taggatacaa ccgtgcagga cgcatcatcg atcaactgga
     6601 agccgccggc attgtcgggc cttttgaagg aagcaaagcc agggaagtac ggtacaaaga
     6661 catggaagct ttggaacagt ttttgaatac cttaagaaac aacaactgac acaccatgaa
     6721 aaaaatattc gtcaccgccc ttttgattag tgtagcggct gtgataaatg cacagaacaa
     6781 cacacaagca aaaacaatac tggatggggt ttcgcaacaa accaaatcct actcaagcat
     6841 tgatatttcg tttagtttta ccgttcaaaa cacagcaaaa aaaactaaag acaccaagtc
     6901 aggattcgcg agcatgaaag gcgaaaaata ctggagcgat tttgcagggc aggaagtcat
     6961 gtgtgatgga aaaaccgtgt ggacctacct gaaagaaagt aacgaagtac aaataaacac
     7021 caacgagcct gacaacgatc aggccctgaa tccactaaat ctgcttaacg attattcaaa
     7081 aagctatacg ccaaagttta tcaaagaaga aacccgcggc tcacgcatat ttcagattct
     7141 ggatctcacc ccggtaaaag caaagtcata ttacaaggta aggctcgaaa tagataaggc
     7201 tgccaaacat attgtcaatg ccattatcta cgataaaaac ggcgtgaaca cctataccta
     7261 ttgcatcacg aagtttgtta ccaataaaac aatacccgaa agcaaattca ctttcaaagc
     7321 ctcagaccat ccgggtgtgg aagtgattga cctaaggtaa cccggtttcc agttgagaca
     7381 ttagcctgtc atgttttttg aagtttaata a
//
LOCUS       DAOSFP010000081         7368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8723_length_7368_cov_4.416382, whole genome shotgun sequence.
ACCESSION   DAOSFP010000081 DAOSFP010000000
VERSION     DAOSFP010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8723_length_7368_cov_4.416382"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PLI16_03625"
     CDS             <1..495
                     /locus_tag="PLI16_03625"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOH83677.1"
                     /translation="FSANTPESLESLRKALFGEELSYATSNHSYNRTGKTEGVLTGGN
                     LSILYSLSGSASDIETNGKILFIEDLEEYLYHIDRMMVQLKRSGKLQNLAGLIVGGMN
                     NMNDNTIPFGKTAYEIIAEHVSAYDYPVCFGFPAGHLDDNRALILGRKITLSVADQTD
                     VVFG"
     gene            complement(573..2003)
                     /locus_tag="PLI16_03630"
     CDS             complement(573..2003)
                     /locus_tag="PLI16_03630"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOH83678.1"
                     /translation="MAAKNGLFLYNWKEYIEISPENYFFISLYRNRYLIAGNVNGFLY
                     IDLDAFYSYNPDCWKFFDHNNGFSGIECGQNGTCIDSRGNVWIPTSESVVKFMPGRLT
                     KNKVPPFLILYSFEISGRNLMWEMAGDESNDTNQTIQIDYTYRNIRIAFHGVSYSCPE
                     KVLYKTRLLGFNDAWSDSTAENSVVFTNLSYGHYVFEVLACNADGVWTEKPMKVHFVI
                     HAAFWQTEWFITIMSLIFAFVIGIVVYLIMQAKRKKELQQLAIEKELISLRVKTINAQ
                     IDPHFVFNAITAIGAEVQVNNVEKAYSYFVKVSNLLRNSLQNNDKILRPLYDEIDFIN
                     NYLTLQKFRFGERFDYTVNVSNHVDMKFEIPKMCVQTFVENALKHGLEHKKSLGRLKI
                     EITMDNRYLVAEITDDGVGREKAGKLNIKSTGIGLKTLKKFFDIYNSYNTENAGFEIT
                     DLFDKMGEAAGTTVKLTIPLSYKFVI"
     gene            complement(2034..3509)
                     /locus_tag="PLI16_03635"
     CDS             complement(2034..3509)
                     /locus_tag="PLI16_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH83679.1"
                     /translation="MRNAIRYIFGVLILLYGSAAFAQQYTYRHYTVKDGLAQNQVTTL
                     YLDKQGFVWVGTKGGASRFDGMKFKNFTMEDGLTDNYIERFLELNGTLFCLTLKGVSV
                     YQNEKFNIIAKFPKSNLNRIVLSSDSSKAYVSFGNKILMTNGKTHEIIYTHDTKDDIY
                     NLVLDPGSNNLIFSVEKKLFCLEPGKKPQIIFDNSHYKLFHDNHRVYFIAEVNQTDVD
                     KNGLYKLVGRQAVKIFDNKNSTLGECHFIASKGHIFMYNNNTCWMEIDTAGHVVDRDS
                     LHGRFFWAFICDKEGNYWLGSETGLFRTQSFAFRNYDEKSGMPLYIWSILESPDSAMV
                     FAGFNGDLSVLKDNKISIIPPAAYPLERRNRFYMNGFCSGKGMWMLPTDGGYVLTFDG
                     KKFDRLFFNIADKYPAILCMHEDTAAHRIYLGTNEGLIIYDTDKKTFKVHPTEKHNVL
                     FVEGDKYGRKWICTNKKIYLFEKDSLKELNLRGFPNDAGVI"
     gene            3737..5434
                     /locus_tag="PLI16_03640"
     CDS             3737..5434
                     /locus_tag="PLI16_03640"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOH83680.1"
                     /translation="MKRVFVAFTCFFIAAFCLQQPAYACTNFIITKGATTDGSVMICY
                     TADSHQLYGELYFRAAQDYPEGALMDVYEWDTGEYRGKIKQARHTYSVVGNMNEHQLA
                     IGETTYGGRSELEDSIRSGIMDYGSLIYIALQRCKTAREAIKLIGELIAEYGYCSSGE
                     SFSIADKNEAWIFEMIGKGVGNKGAVWVARRIPDGYISGHANQARITTFPLADGKTSI
                     TNLQFKKIDLPTIECIYSSDVIEFARTKGYFTGKDAEFSFADAYAPVDFGAARFCEAR
                     VWAGFNRFSKEMAQYQDYAMGENLKNRFPLWIKPDRKLSVYDVMEIMRDHFEGTPMDM
                     TNDLGAGPFKCPYRWRPMTWSQDGGDYIHERATSTQQTGFSFVAQCRGWLPDAIGGII
                     WFGVDDTYSTVYAPMYCGITKVPESYAVGLGDMVTFNDNAAFWVFNQVSNFAYTRYCD
                     IIPEIRTLQKELETKYIQTEVPETDKNALALYKENPAKALELITQYSVKTGNNLVARW
                     KELYKYLFVKYLDGNVKYPGKTKESSNVDHPRYSDDWYRMIIKETGDRYKVKSGSAQ"
     gene            5462..7186
                     /locus_tag="PLI16_03645"
     CDS             5462..7186
                     /locus_tag="PLI16_03645"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH83681.1"
                     /translation="MRLFYTPMPLLDLQHLNIDFKGENGVVHAVSDVSFTLEKGKTIG
                     IVGESGSGKSVTSLALMGLLPSGISKITSGQALFTDKTGNITDILALPEKNKRKFRGA
                     ELAMIFQEPMTSLNPVYKCGRQVDETIMLHQHVSKKEAKRLTLEWFKEVMLPDPVKAY
                     RSYPHELSGGQRQRVMIAIAMSCTPSLLIADEPTTALDVTVQKNIIELMKHLQQKYQM
                     SIIFISHDLGVIAEIADYVLVMYKGIVVEQGAVDTIFENAQHPYTKGLLACRPKLGIR
                     YKQLPTVERFMKNNIDGIFDEIPPEEHRQELEKKYENKPILEAVNLKTHFSIKRGWFQ
                     TPSVFKAVDDVSFTVFPGETLGIVGESGCGKTTLGRTLLRLVEPASGKIVFFGSEISS
                     YSKSKLRKLRKDFQIIFQDPYSSLNPRLTVGKAISEGMRIHGLGANATERKKTVLDIL
                     NKVNLGDTIYDRYPHELSGGQRQRISIARALALRPKFIICDESVSALDVSVQAQVLNL
                     LNELKKEYRFSLIFISHDLSVVKYMSDRMMVMKNGKIVEYGPADEIYKNPKTDYTKNL
                     IASIPKGL"
     gene            complement(7181..>7368)
                     /locus_tag="PLI16_03650"
     CDS             complement(7181..>7368)
                     /locus_tag="PLI16_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005850133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOH83682.1"
                     /translation="FVNEKYDAGAVIFQARCPVTPEDTAETIAHKVHQLEYRYFPEII
                     EKVIAGKAGRPSASSVL"
BASE COUNT         2181 a         1673 c         1481 g         2033 t
ORIGIN      
        1 ttctctgcca acactcctga atctttggag agcctcagaa aagccctgtt tggagaagaa
       61 ctttcttacg ccacatcgaa ccatagctat aacaggacag gtaaaaccga aggtgtatta
      121 actggtggca acctgtcaat tttatattcc ctgagtggtt ctgcttctga tatagaaacc
      181 aatgggaaaa tactttttat tgaggacctt gaagaatatc tttaccacat cgatcggatg
      241 atggtacaac taaaacgcag cggcaagctg caaaaccttg cagggcttat tgttggcggg
      301 atgaataata tgaacgacaa caccatacct tttggaaaaa cggcctatga aattatcgcc
      361 gaacatgtgt cagcctatga ttatccagta tgtttcgggt ttcccgccgg gcatcttgac
      421 gacaaccgcg cgctgatttt aggacgaaaa ataactcttt ctgttgcaga ccaaacagat
      481 gtagtgtttg gctgaatcca gattacccgg atgcaacgca caatccctcc cactgcacaa
      541 tagttcacta tgttcctttt gcgcaatctg ggttaaataa caaatttata gctcaacgga
      601 atggttagtt tcaccgtggt tccggcagct tctcccatct tatcaaacaa atcggtgatt
      661 tcaaagccgg cattttcggt attgtatgaa ttataaatat caaaaaactt cttcagggtt
      721 tttaacccaa tccctgttga ttttatattc aacttccccg ctttttccct cccaactcca
      781 tcatcggtaa tttcggccac cagataccta ttgtccatag taatttcaat tttcagccgc
      841 ccgagcgact ttttatgctc caagccgtgt ttcaaagcat tttcaacaaa agtttgtacg
      901 cacatcttgg gaatttcaaa tttcatgtcg acgtggttcg aaacgtttac cgtataatca
      961 aaacgttcgc caaacctgaa tttctggagg gtaagataat tattaataaa atcaatttca
     1021 tcatacaacg gacgcagaat tttatcgttg ttttgcaatg aattgcgcaa caaattggac
     1081 actttaacaa aatatgaata ggccttttcc acattgttta cctgtacttc ggcaccgata
     1141 gcggtgatgg cattaaatac aaagtgggga tctatctgtg cattaatcgt tttaacccgc
     1201 aaactgatca gttctttttc tattgccaat tgctgaagtt cttttttcct tttggcctgc
     1261 attattaaat aaacaacgat accaatcaca aacgcgaaaa ttaacgacat gatcgtaatg
     1321 aaccactcgg tttgccaaaa tgccgcatgt ataacaaaat gcaccttcat cggtttctct
     1381 gtccacacgc catcagcatt gcatgccaaa acctcaaaaa cataatgtcc atacgaaaga
     1441 ttggtaaata ccacgctatt ttcggcggtt gaatccgacc atgcatcatt aaaccccaat
     1501 aatcttgttt tgtacaatac tttttcggga catgaatacg aaacgccatg gaaggctatc
     1561 ctaatattgc gatatgtata atcaatttga atggtttggt tcgtatcgtt tgactcatcg
     1621 ccagccattt cccacataag atttcttccg gatatttcga aagaataaag aataagaaaa
     1681 ggcggaactt tgttttttgt aagccgcccg ggcataaatt ttaccacact ttctgaggta
     1741 ggtatccaca cattgccacg gctatcgata catgttccgt tttgtccgca ttctatcccg
     1801 gaaaagccgt tgttatggtc gaaaaatttc cagcaatcgg gattataaga ataaaaagca
     1861 tccagatcga tatatagaaa accattaaca ttgccggcta ttaaataacg gttgcgataa
     1921 agactgatga aaaaataatt ttcgggtgaa atttctatgt actctttcca gttataaaga
     1981 aaaaggccat ttttggctgc cacccacata ttgccccgcg aatctctttt acactaaatt
     2041 acccctgcat catttggaaa acctcgcaaa ttcagttctt tgagggagtc tttttcaaac
     2101 aaataaattt ttttgttggt acaaatccat tttctgccat atttgtcgcc ttccacaaac
     2161 agcacattgt gtttttctgt aggatgaacc ttaaaggttt ttttatctgt atcataaatt
     2221 atcaaccctt cgttggtgcc cagatatata cggtgtgcgg cagtatcttc gtgcatacac
     2281 aaaatggcag gatatttatc tgcaatatta aaaaacagcc tgtcaaattt tttcccatcg
     2341 aaagtaagca cataacctcc gtcggtgggc aacatccaca ttcccttgcc ggagcaaaac
     2401 ccattcatat aaaaccggtt cctcctttct aatgggtatg cagcgggagg gatgatagaa
     2461 attttattat cttttagcac gctcaaatca ccgttaaacc cggcaaaaac catggcgctg
     2521 tcgggacttt ccaaaatact ccagatataa agtggcatgc ctgatttttc gtcatagttt
     2581 ctgaaagcaa atgattgtgt gcgaaaaagg ccggtttcgg atcctaacca ataattgcct
     2641 tctttatcgc aaataaaggc ccaaaaaaac ctgccgtgta aagaatctcg gtcaacaaca
     2701 tgcccggcag tgtcaatttc catccagcat gtgttattgt tgtacatgaa aatgtgcccc
     2761 ttggatgcaa taaaatgaca ctctcccaat gtggaatttt tattatcgaa tatttttacc
     2821 gcctggcggc caaccaattt atataatccg tttttgtcca catcggtctg gtttacctct
     2881 gctataaaat aaacccggtg gttgtcatga aaaagtttat aatgcgaatt gtcaaatatt
     2941 atttgcggct tttttcccgg ctccaggcaa aataattttt tctcaactga aaaaatcaaa
     3001 ttattgctgc ccggatccaa cactaaattg taaatatcat cctttgtatc gtgggtataa
     3061 ataatttcat gggttttgcc attggtcatt aaaattttat ttccgaagct cacataagcc
     3121 ttgcttgaat cgctggacag cacaattctg tttaaattac tttttgggaa tttcgctatg
     3181 atattaaatt tctcgttctg ataaaccgaa acacctttta aggttaaaca aaacagcgtg
     3241 ccgtttaatt ccaaaaacct ttcaatataa ttatccgtca acccatcttc catggtgaaa
     3301 tttttaaact tcatcccgtc gaaacggctg gcgccgcctt tggtgccaac ccacacaaac
     3361 ccctgcttat ccaggtataa agtggttacc tggttttgcg ccaggccatc cttcactgta
     3421 taatgcctgt aagtatattg ctgcgcaaag gcagcagagc catataataa aatcaatact
     3481 ccaaaaatgt atcgaatggc gtttcgcata atcggcaaat ataaaatatt tgagtttatt
     3541 ttaactcatt cattcgtaat aaataaaaaa atttatataa attacaaata taattgtttc
     3601 cttgactatt tcgccgacaa gactgctgtt tcgcggggaa gcatatcctc aaaataaaaa
     3661 atcatcgttc ctgtagcgaa aaaataagta tttttggaga gaattatcaa agttattaat
     3721 caaacccaca atgcttatga aaagagtttt tgttgcattt acctgttttt tcatcgctgc
     3781 tttttgctta caacagcctg cttatgcatg cactaatttt atcatcacca agggagctac
     3841 taccgatggc tcggtgatga tttgctatac cgccgactcg caccagcttt atggtgaatt
     3901 gtatttccgg gctgcacagg attaccccga aggcgccctg atggatgttt atgaatggga
     3961 taccggcgaa taccgtggaa aaataaaaca agcccgccat acttattccg tggtgggcaa
     4021 tatgaatgaa caccagcttg ccattggcga aaccacctat ggaggcagat ccgaactgga
     4081 agattccatc agatcgggaa ttatggacta tggcagcctg atatacatag ccctccaaag
     4141 atgcaaaaca gcccgtgaag ccataaaact tatcggcgaa ttgattgccg aatacggcta
     4201 ttgtagttcg ggagaatcat tttctattgc cgataaaaac gaagcctgga tatttgaaat
     4261 gattggtaaa ggtgtaggta ataaaggcgc cgtatgggta gcccggcgta ttcctgatgg
     4321 ttatatcagc ggccatgcca accaggcacg tatcaccacg tttccgctgg ccgatggcaa
     4381 aacgtctatc accaacctgc aattcaaaaa aattgacctg cccaccattg aatgtatcta
     4441 ttcttcagac gttattgagt ttgcccgcac taaaggttat tttaccggca aagatgccga
     4501 attcagtttt gccgatgctt atgctccggt agattttggc gctgcccgtt tttgcgaagc
     4561 ccgtgtttgg gccggattta accgtttcag caaagaaatg gcgcaatatc aggattacgc
     4621 gatgggcgaa aacctcaaaa acagatttcc gctatggata aaacccgaca gaaaactctc
     4681 ggtttatgat gtgatggaaa ttatgcgcga ccattttgaa ggcaccccga tggatatgac
     4741 caatgatctc ggcgccggcc cctttaaatg cccttaccgc tggcggccta tgacctggag
     4801 ccaggatggc ggtgattata tccacgaaag agccacctcc acacaacaga cagggttctc
     4861 ttttgtggca caatgcagag gctggttacc cgatgctatc ggtggtatca tctggtttgg
     4921 cgttgacgac acctacagca ctgtatatgc acctatgtat tgcggcatta ccaaagtccc
     4981 tgaatcctat gctgtcggcc tcggcgatat ggtaaccttt aacgacaatg ccgcattctg
     5041 ggtattcaac caggtttcaa actttgccta tacccgttat tgcgacatca tccccgaaat
     5101 acgaaccctg caaaaagaac tggaaacaaa atatattcaa accgaggttc ctgaaacaga
     5161 taaaaacgct ttggctcttt acaaagaaaa tccggccaaa gctttagaac taatcacaca
     5221 gtattctgtg aaaaccggaa acaatctggt ggcccgctgg aaagaacttt acaaatacct
     5281 atttgtaaaa tacctggacg gaaacgtgaa atatcccgga aaaaccaaag agtcgtctaa
     5341 tgtagatcac cctaggtaca gcgacgactg gtaccggatg attatcaagg aaaccggcga
     5401 ccgttacaaa gtaaaaagcg gcagcgctca gtaaaaataa aaccatacag ccgcaacata
     5461 gttgcggctt ttttatacac ctatgcccct gctcgacctg caacatctga atattgattt
     5521 taaaggcgaa aacggcgtgg ttcatgccgt cagcgatgtt tcttttaccc ttgaaaaagg
     5581 caagaccatc ggcatagtgg gcgaatccgg atcgggaaaa tcagtaacct cgctggcatt
     5641 gatgggcctg ttgccgtccg gcatttcaaa aatcacttcc ggacaagccc tgtttaccga
     5701 taaaacaggc aacattacgg acatcctggc cctgcccgaa aaaaacaaaa ggaaattcag
     5761 gggcgccgaa ctggccatga tttttcagga gccgatgacc tcgcttaatc ccgtttacaa
     5821 atgtggccgg caggtggacg aaacaattat gctgcatcag catgtgagta aaaaagaagc
     5881 caaacgattg actcttgagt ggttcaaaga agtcatgctg cccgaccccg tgaaagccta
     5941 tcgttcctat cctcatgagt tgtcgggagg acaacgtcag cgcgttatga tagccatagc
     6001 catgagttgc acgccttcgc tgctgattgc cgatgagccc accaccgcac tggacgtaac
     6061 cgtgcagaaa aacatcatcg aactgatgaa gcacctgcag cagaaatacc agatgagcat
     6121 aatttttatc tcgcacgacc tgggtgttat cgctgaaata gcggactatg tgctggtgat
     6181 gtacaaggga atcgtagtgg aacaaggcgc tgtggataca atttttgaaa acgcccaaca
     6241 tccttacacc aagggactgc ttgcctgccg tcccaaactg ggaatacggt acaagcaact
     6301 gcccacggta gaacgtttta tgaaaaacaa tattgatggt atttttgatg aaataccacc
     6361 cgaagaacac cggcaggagc tcgaaaagaa atatgaaaac aagcccatac tggaagctgt
     6421 aaacctgaaa acacattttt ccataaaacg gggatggttt cagactcctt ctgtttttaa
     6481 agccgtggac gatgttagtt ttacggtatt ccccggcgaa actcttggta tcgttggcga
     6541 atcgggttgc ggcaaaacca ccctgggaag aaccctgttg agactggtgg agccggcatc
     6601 agggaaaatt gtttttttcg gaagtgaaat cagttcttat tccaaaagca aactgagaaa
     6661 gctgcgtaaa gattttcaga taatttttca ggacccctac tcttcgctta acccgcggct
     6721 gaccgttggc aaggcaatta gcgaaggtat gcgcatacat ggattgggcg ccaatgcaac
     6781 ggaaagaaaa aaaactgttt tagacattct caataaagtt aaccttggcg acaccattta
     6841 cgaccgctac ccgcacgaac tctcgggcgg ccagcgccag cgcatctcca tagcaagggc
     6901 gctggccctc aggccaaaat ttattatctg cgacgaatcg gtttcggcgc tggatgtctc
     6961 agtacaggca caggtattga acctgttgaa tgagttgaaa aaagaatacc ggttttcgct
     7021 gatatttatt tcgcacgatc tttcggtggt aaaatatatg tcggaccgca tgatggtgat
     7081 gaaaaacggt aagattgtcg aatacggccc tgccgacgaa atttacaaaa acccaaaaac
     7141 agactatacc aaaaacctga tagcctccat cccaaaaggc ttataaaacg gaacttgcgg
     7201 agggccttcc tgctttcccg gcaattacct tctcgattat ttcgggaaaa taccggtatt
     7261 ccaactgatg aaccttgtgc gcaattgttt ctgccgtatc ctccggtgta acagggcaac
     7321 gcgcctgaaa tattacagct cccgcatcgt atttttcatt gacgaaat
//
LOCUS       DAOSFP010000082         7272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8900_length_7272_cov_3.648192, whole genome shotgun sequence.
ACCESSION   DAOSFP010000082 DAOSFP010000000
VERSION     DAOSFP010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8900_length_7272_cov_3.648192"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="PLI16_03655"
     CDS             complement(<1..1000)
                     /locus_tag="PLI16_03655"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83683.1"
                     /translation="MKKIYSIILLFFALYSQSQVVVDSTDMAVPGDILKFRTVNNLSG
                     FNYQQTDTAYTWDFSALAGTSETIDTFISVNSTNIAYVAVYANPLDQANKATVAQPVK
                     MQTIPMVSITESFNFYKNNFSRFSMLGMGTKVNNIPVPLKFDHPDVLYYFPLTYGAKD
                     TSDSEVHANIPGLGYYGHTVHRENFVDGWGTLYLPADTFEVIRVKSKVIYYDTIYYDS
                     IGFGGGTTRNVTEYKWLADGFHEPVLQITKSGQNNVSAKYFNHIPVDLAVETIDNIGN
                     LDIYPNPASDFITLEIPVTMDEICLTISDITGRQITKRSFLRQQRIELPVGQLQKGI"
     gene            complement(1116..1664)
                     /locus_tag="PLI16_03660"
     CDS             complement(1116..1664)
                     /locus_tag="PLI16_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH83684.1"
                     /translation="MEKRAKKTTKQLIKVAITGPESTGKSMLAEQLAAYYHTVFAPEY
                     ARNYIDRINREYTFEDLAIIAKGQIAAERDAEKNADKILFCDTELTVIKIWSEHKYNM
                     CPEWVAANLTKFTYDLYLLCDIDLDWKYDPLREHPHLRSYFFDWYYKELKDRNVPFKV
                     VKGKGAERLKNALFFTEELLSK"
     gene            complement(1628..2212)
                     /gene="pnuC"
                     /locus_tag="PLI16_03665"
     CDS             complement(1628..2212)
                     /gene="pnuC"
                     /locus_tag="PLI16_03665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOH83685.1"
                     /translation="MNWITENYIEIIAAILGIAGVYLTTKQKIWCWPVGLANVMLSIY
                     VFFVSKLYADVVLQIFYLAMTLYGWYFWAFGGEKKYELPVRKIRPKELSVMLVLGFVC
                     SFVVGYLFANYTDASFPYWDSLLTVWGIIATYAMGKKIIDHWLMWIVIDINCTVLYFI
                     KGLYAFAPLYFIFTLLAIYGYFTWKKELKKQQNS"
     gene            complement(2650..2895)
                     /locus_tag="PLI16_03670"
     CDS             complement(2650..2895)
                     /locus_tag="PLI16_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83686.1"
                     /translation="MKISVEISYYPLKEEFKPHIIDFLRRINAYKELTAQTNGMSTQI
                     FGEYKYVMNALTNEIEKSFALPHSVFVMKIINADLKA"
     gene            3050..3604
                     /locus_tag="PLI16_03675"
     CDS             3050..3604
                     /locus_tag="PLI16_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83687.1"
                     /translation="MKRIFLFLAIGVFAMFLVSCLTVEKKQYTYEFTGKGGGKLTIKY
                     INIMSSEDSAGYSRVEDFDDLLGSYINGSKIEEAYPEATNISKRLFEENGVLCGEVIM
                     DFASLSAARLYQFDKKSPYMFYASSVDGETYVESNGTYGGENMPVVMFPSKLKKLVIT
                     TSVSQPSDDSYVSLLGEYVKWKKK"
     gene            complement(3669..4172)
                     /locus_tag="PLI16_03680"
     CDS             complement(3669..4172)
                     /locus_tag="PLI16_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83688.1"
                     /translation="MKTKKSTETILVLFVSACLMICSGNLFAQNTGNKNGDPEKKEKI
                     KAMKVAYISQMINLNTAEAEKFWPLYNEFQDKREGFQKEHQKKMKIMKTGKIEELTEE
                     QADEIINSELQKEQKQLDLKKEYYPKFKNVIGSRKMVGVYKAEKEFNRMLLEKLKGNG
                     PKPGLDD"
     gene            complement(4194..4742)
                     /locus_tag="PLI16_03685"
     CDS             complement(4194..4742)
                     /locus_tag="PLI16_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83689.1"
                     /translation="MKKKHHITHNTDMHEDFSENQQKFTPGGNPFKVPENYFEELPQR
                     IQERVSMSTRTDTKKTPVVKLLLYSAATAAAIGAVMLVFWITRPPSNEKKLISHESTI
                     DADQITAYLEDEVDENTLIEVCSDRAAIFSPEEIPDISLPAYENETPQNPEPAITFDT
                     SINKNDIIDYLISENIEPETFQ"
     gene            complement(4779..5327)
                     /locus_tag="PLI16_03690"
     CDS             complement(4779..5327)
                     /locus_tag="PLI16_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH83690.1"
                     /translation="MQPVPDKEIIQLIKNDASRHQGFELLVRGYSEKTYRLIRRIVIT
                     HEDSDDVVQNVFIKIWNNIDNFRADAQLSTWIYRIAVNEALIFLKHKKMFHFIPYFNV
                     EKKLANTLKDDNFFKADEVEMHLHKCILSLPERQRLVFNMRYYEEFSFEEISKILNTS
                     TGSLNASYHSAYQKIKKNLTAL"
     gene            5436..5840
                     /locus_tag="PLI16_03695"
     CDS             5436..5840
                     /locus_tag="PLI16_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83691.1"
                     /translation="MKKLFLFTIASLLIVACTSKHEKAVELNKSGIRKMYSQQVDEAL
                     KDFDKAIELDPLFDQPYYYRGNIKYGATDYQGAQEDYSKAIELNPGFADAYYNRGNLY
                     ESINQRDKACADWLKAKENGKANLDDKLRFCR"
     gene            complement(6145..7242)
                     /gene="ychF"
                     /locus_tag="PLI16_03700"
     CDS             complement(6145..7242)
                     /gene="ychF"
                     /locus_tag="PLI16_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOH83692.1"
                     /translation="MGLNCGIVGLANSGKTTIFNCMSSTKAQITNFAFSSNKSNIAIV
                     NVPDERLYVLEKLVKAAKVVHATVEIVDIPGLTKGSSKGEGMGNSFLADIRQTDAIIH
                     VIRCFDDENLPHIEGSVNAVRDKEIIDLELQINDLEQVERKMQRTEKAAKTGDKEAKH
                     LLEVLTKYKNHLESFQSARTLDIADEERRLVKDLCLLTEKPVLYVCNVDDGSAVGGNK
                     YSEALTEAVKNENTEVLVIAGKLEAEISELETTEDRMAFLNDVGLTEPSVNKLIKSAF
                     TLLNLRTFLTAGPKEVRAWTIKTGMNAQQAAGAIHSDLERGFIRAEVIKYHDFVKYGS
                     ENACKEAGKFNVEGKTYIVEEGDLMNIRFNV"
BASE COUNT         1991 a         1436 c         1520 g         2325 t
ORIGIN      
        1 atattccttt ttgaagttga cctacaggga gttcaattct ttgctgcctg agaaaacttc
       61 tttttgttat ttgtctgccg gtgatatccg aaatggtcag gcatatttca tccatggtaa
      121 caggaatttc gagggtaata aaatcgctgg cagggttggg atagatgtca aggttgccaa
      181 tgttgtcaat ggtttcaacg gcaaggtcaa ccggtatgtg gttgaaatat ttggccgaaa
      241 cattattttg cccgcttttg gttatttgca acactggttc gtgaaaacca tcggcaagcc
      301 atttgtattc ggttacattg cgcgtggttc cgcccccaaa tccgatggaa tcataataaa
      361 tggtgtcata gtaaattact tttgatttta ccctgattac ttcaaaagta tcagccggaa
      421 gatacaaagt tccccatccg tcaacgaaat tttcgcggtg cacggtatgc ccgtaatagc
      481 ctaatccggg aatattcgca tgaacctcag agtctgaagt gtccttggct ccgtatgtca
      541 gcggaaaata atacagcaca tcgggatggt cgaatttcag aggaacaggg atattattga
      601 ctttcgtgcc cataccaagc atactgaagc gggagaaatt atttttatag aaattgaagc
      661 tttcggtgat agataccatg ggaatagtct gcattttcac tggttgtgct actgtggctt
      721 tgttggcctg gtcgagcgga ttggcatata cggcaacgta ggcaatgttg gtggaattga
      781 cactgatgaa agtatctatg gtttcggaag tccctgccag tgccgaaaag tcccaggtat
      841 aagccgtatc ggtttgttgg taattaaacc cgctgaggtt gtttactgtc ctgaacttta
      901 atatgtcgcc aggcacagcc atatcggtgc tgtcaacaac cacctgcgac tgactataca
      961 atgcaaaaaa cagtaagatt atggagtaga tttttttcat aaaaaggttt tttcaaaggt
     1021 aataatatta ttgtgattat tgataagcag gtaagcaaca gaaaaatatg tcagctacag
     1081 ccgcttgatt ttgcgatgac ttaggcagta cctggttatt tggaaaggag ttcttcagtg
     1141 aaaaacaggg cgtttttcaa tctttcggca ccttttcctt ttaccacttt gaacgggaca
     1201 tttctgtctt ttagttcctt ataataccaa tcaaagaaat aggagcgaag gtgcgggtgt
     1261 tcgcgcagcg ggtcgtattt ccaatccaaa tcaatgtcgc acagcaggta caggtcatac
     1321 gtgaatttgg tcaaatttgc tgctacccat tccggacaca tgttgtactt gtgttcgctc
     1381 caaattttta ttaccgtgag ttccgtgtcg caaaacagga ttttatccgc gtttttttca
     1441 gcatcccttt cggcggcaat ctggcctttt gcaataatgg caaggtcctc gaaagtatat
     1501 tccctgttaa tccggtcgat gtagttacgc gcgtattcag gggcgaagac ggtatggtaa
     1561 tacgccgcca attgttctgc cagcattgat tttcctgtcg attcagggcc ggtgatggca
     1621 acctttatta gctgttttgt tgttttttta gctctttttt ccatgtgaaa tacccgtaaa
     1681 tggccaacaa ggtaaaaata aaatacagag gcgcaaaagc atagagtcct ttgatgaagt
     1741 ataatactgt acagttgatg tcaatcacta tccacatcag ccagtgatcg atgatttttt
     1801 tgcccatggc ataggttgca atgatgcccc acacggtgag taatgaatcc cagtaaggaa
     1861 atgatgcatc ggtatagttg gcaaaaagat acccgacaac aaaagaacat acaaatccca
     1921 gaaccagcat gacgctaagt tcttttggcc ggattttccg caccggaagc tcgtattttt
     1981 tttctccccc aaatgcccag aaataccatc cgtaaagcgt catagcgagg taaaaaatct
     2041 gaagcaccac gtcggcataa agttttgaaa caaaaaaaac ataaatcgac agcatgacgt
     2101 tggcaaggcc tacaggccaa caccaaatct tctgtttagt ggtcagatat acgcctgcaa
     2161 taccaaggat ggcggcaata atctctatgt aattttcggt gatccagttc ataggggaaa
     2221 aaagggaagt aaaaagttta aagttgcaag taataagttg aaagtagcaa gttaaaagtt
     2281 ataagttgtc agaagtcaga attcagaatt atgaagtcag aattatgaat ttagtaatca
     2341 ttaatcatta gtcattaatt agaagtcaga attatgaagg cagaattaag aagtcattaa
     2401 tcagttgaaa gttgaaaata acaagttgaa aataacaagt tgaaagtgac aagttaaaag
     2461 tgacaagtta aaagtagtca ttccgatagt ctctagtctc caatctatag tctccaatct
     2521 ccaatctcta atctctaatc tctaatctct aatatccaat cttcaatttc tgttctctgt
     2581 aacctgttca ttgtccattg tgctccctgt tcattgttca ttgttaatcg agtcggggct
     2641 ttagtgatgt tatgctttta aatcggcatt gattattttc attacaaata cggagtgtgg
     2701 cagggcgaag gatttttcaa tttcgttggt cagggcattc atcacatatt tgtattctcc
     2761 aaagatttga gtactcatgc cgttggtctg cgctgtaagc tctttatatg cattgatacg
     2821 tctcaggaaa tcaatgatat gaggtttaaa ttcttctttt agcggataat aacttatttc
     2881 tacagaaatc ttcataaaaa attttttgag caaaggtaca tcgaaaaaaa ttactatttt
     2941 tgaaaagtaa atcctgaatg aataaatccc tgcggtaaaa ttatgtataa aaggtaagat
     3001 gttgcattac aggaattggg attgcatccg gccttataaa accaaacaga tgaaaagaat
     3061 atttttattt ctcgctattg gagtatttgc catgtttctg gtaagttgcc ttaccgtaga
     3121 aaagaaacag tacacttatg agttcaccgg taaaggaggt ggaaaactta ccataaagta
     3181 cattaatatc atgtcgagtg aggattctgc cggatattca agagttgaag actttgatga
     3241 tttgcttggc agctatatca acggttcgaa aatagaagaa gcatatcccg aagctactaa
     3301 catttcgaaa cggctcttcg aagaaaacgg agttttatgc ggagaagtca tcatggattt
     3361 tgccagcctc agcgctgccc ggctttatca gttcgacaaa aaaagtccct atatgttcta
     3421 tgccagctct gtggacggcg aaacctacgt ggaatcaaac ggaacctacg gcggcgaaaa
     3481 tatgccggtg gtcatgtttc catccaaact gaaaaaactg gttattacca ccagcgtcag
     3541 tcagccatcc gatgatagtt atgtcagcct gttgggcgaa tatgtcaaat ggaagaaaaa
     3601 ataatagcgg aaataaataa aaaaggctgc ccgttgttct caggcagcct tttttttgga
     3661 attatttctc agtcgtcaag gcctggcttt ggcccgttgc ctttgagttt ttccagcagc
     3721 attctgttaa attctttttc agctttgtaa acgccaacca tcttcctgct gccaataaca
     3781 tttttaaact ttggataata ttcttttttc aggtcaagtt gcttttgctc tttctgtaat
     3841 tccgagttaa tgatctcatc ggcttgttct tcggtgagct cttcaatttt accggttttc
     3901 atgattttca tttttttctg atgttctttt tgaaaacctt ctcttttatc ctgaaactcg
     3961 ttgtataaag gccagaattt ttctgcttca gcggtgttca ggttgatcat ctggctgata
     4021 taagccactt tcatggcctt tatcttttct tttttttccg gatcaccgtt tttgtttcct
     4081 gtgttttgtg caaagaggtt tccgctgcat atcatcaggc atgctgatac gaacaaaacc
     4141 aaaattgttt ctgttgattt ttttgttttc atgatggttt attttaaatt gttttattga
     4201 aatgtttccg gttcaatgtt ttcgcttatc aagtaatcga tgatgtcatt tttattgata
     4261 gaggtgtcga atgttatggc aggctccgga ttttgcggcg tttcattttc ataagccgga
     4321 aggctgatgt cgggaatttc ttcaggacta aaaatggcag cacggtcgct gcaaacctca
     4381 ataagagtat tttcatcaac ctcgtcttcc aggtaggcag tgatttggtc ggcatcgata
     4441 gttgactcat ggcttatcag ttttttttca tttgatggcg gcctggtaat ccaaaaaacc
     4501 agcataacag ctccaatggc ggccgctgtg gctgccgaat acaataacaa tttcacaacc
     4561 ggtgtttttt ttgtgtcagt cctggtggac atgctcactc tttcctgtat tcgttggggc
     4621 agttcttcaa aataattttc aggcacttta aacggattgc cgcccggggt gaatttttgc
     4681 tgattttcag aaaaatcctc gtgcatatcc gtattgtgtg ttatgtgatg tttctttttc
     4741 atgccggtga taataagatt aatgaaacgg taaaaggttt aaagggctgt aagatttttt
     4801 tttattttct gataggcact gtggtaggaa gcgttcagtg agcccgtgct ggtattaagg
     4861 atttttgaga tttcttcaaa agaaaattct tcgtaatacc tcatattgaa caccaggcgt
     4921 tgtcgttcag gcaaggaaag tatgcattta tgcaaatgca tttcaacctc atcggcttta
     4981 aaaaaattat cgtctttcaa ggtgttggca agtttttttt ccacattaaa ataggggatg
     5041 aagtgaaaca tttttttgtg tttaagaaaa atcagcgcct cgttcacagc tatcctgtat
     5101 atccaggttg acaattgcgc gtctgcacga aaattatcaa tattgttcca tattttgata
     5161 aagacatttt gtaccacatc gtcgctgtct tcatgcgtaa taacgattct tcgtatcagc
     5221 cggtaagttt tttcggagta tcccctgacc agcaactcaa aaccctgatg tcgcgatgcg
     5281 tcgtttttta tcagttgtat gatttcctta tccggcacgg gctgcatttt gttttttttc
     5341 tttagatgtt tgtttctgcc aaaggtttaa aacggcaaat tttttttttg aaaaagttta
     5401 ttgttacttt gcaggtttat tttataacgt atctgatgaa aaaattattt ttatttacta
     5461 tagccagcct gctgattgtt gcctgtactt cgaagcacga aaaagccgtt gagctgaaca
     5521 aaagcggtat ccgtaagatg tacagccaac aggtggacga ggccctgaag gattttgaca
     5581 aagccattga attagaccct ttgtttgacc agccttatta ttacaggggg aatataaaat
     5641 atggggccac cgattatcag ggtgctcagg aagattacag caaggccatt gagctaaatc
     5701 ccggctttgc cgatgcgtat tacaatcgcg gaaaccttta cgaatccatc aatcagcgcg
     5761 acaaggcctg tgccgactgg ctaaaagcca aggaaaacgg taaggccaac cttgatgaca
     5821 aattgcggtt ttgccggtag gcattgcaaa taggcaccgt taataaaatc gaacgggcaa
     5881 agttaacctg aaaaattcat acgaattttt cacccttcgg gccgataaaa gaaaatccgg
     5941 caatcacttc aaaactaaaa tggttaatcg aaattcgtac atctttaatt cctctacaat
     6001 atcgaacacc gattaacgaa tgatgattta agaagtggcg aatcacttca aaaatctaat
     6061 ttcgttaatc gaaattcgta catcattttt ttttaatatc gaacaccgat taacgaatga
     6121 tgatttaaga tttatagagg tgctctaaac attgaacctg atgttcatca ggtcgccttc
     6181 ttccacaata tatgtttttc cttccacatt gaactttccg gcttctttac aggcgttttc
     6241 agagccgtat ttcacaaagt cgtggtattt gattacttct gcacggataa agccgcgttc
     6301 caggtccgaa tgtatagctc ctgcagcctg ctgggcattc atgccggttt tgatggtcca
     6361 ggcacgcact tcttttggtc ctgcagtgag gaaggtgcga aggtttagca gtgtaaatgc
     6421 cgattttatc agtttgttca cgctgggctc ggtaaggcct acatcattca ggaacgccat
     6481 gcggtcttcc gtggtttcca actccgaaat ctctgcttcc agttttccgg caatcaccag
     6541 cacttcggta ttttcgtttt ttacggcttc tgttagcgct tcggaatatt tattgcctcc
     6601 tacagccgat ccatcgtcca cattgcagac ataaagcact ggcttttcgg tcagcaggca
     6661 caaatctttt accaggcggc gttcttcatc ggcaatgtca agggttcgcg ccgactgaaa
     6721 agattccagg tggtttttat attttgtgag gacttccaat aagtgtttgg cttctttatc
     6781 gcctgtttta gcagcttttt cggtacgttg catcttacgt tccacctgtt ccaaatcgtt
     6841 gatttgcagt tccagatcaa tgatttcctt atccctcacg gcattgaccg atccttcgat
     6901 atgaggcagg ttttcatcat caaaacaacg tatcacatga atgatggcat ctgtctgcct
     6961 gatgtcggcc aaaaaagagt ttcccatgcc ttcgcctttg ctggaacctt tggtcagtcc
     7021 cggtatatcg actatttcca ccgtggcatg aaccactttg gccgctttta ccaatttttc
     7081 caggacatac aggcgttcat cgggcacatt gacaatggca atatttgatt tgttagagct
     7141 aaaggcaaaa ttggtgattt gcgctttggt gctcgacata cagttgaaaa ttgtggtctt
     7201 gccactattg gccaatccca ctatgccgca attcagtccc atgtgttttt tttgacaaag
     7261 gtataacttt tc
//
LOCUS       DAOSFP010000083         7268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8908_length_7268_cov_4.553445, whole genome shotgun sequence.
ACCESSION   DAOSFP010000083 DAOSFP010000000
VERSION     DAOSFP010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_7268_cov_4.553445"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLI16_03705"
     CDS             <1..470
                     /locus_tag="PLI16_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HOH83693.1"
                     /translation="AGFIFIGLMNVGGNPYVIEYNVRMGDPETESVLPRVKNDLVELL
                     LATASGTLHEKQLDIDDRYAVTVMMVSEGYPGKYKKGKKIAGLNALDGSTVFHAGTTV
                     DAEGNIITNGGRVLGVMSYGATLTEAREKSYKNTAIIDFEGKYYRHDIGNDLM"
     gene            484..1473
                     /locus_tag="PLI16_03710"
     CDS             484..1473
                     /locus_tag="PLI16_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83694.1"
                     /translation="MFTRIFKYHTNFQIVFILLSALALWLPAFFKGVDMMGTIDLSYG
                     YNTLFFKTYSIPLLYMGLAFILLLAEALVLNTMLVEIKISPKNSYLPAFLYILLMSSS
                     PKLLTLHPCLIVNAFIMIVLMLLFEIKNKDDGYREIFLCGILTGLGSLFYFKAVGLLI
                     IIWIFILLYRPFTWREWVISLLGVATVYVYLFTYFLLTDQLSAVLFDYRRVFSSMSFG
                     GDFRTLSLYQYITLALIVVLFLISSVKMFVLSHEKVIHVRKALYAIFWLLAVNTLSVL
                     FLVKNLMYEFSYTLLPLSILVAYYYLNLKKKLFGEIILGLFIVSIILQKIFIS"
     gene            complement(1466..1948)
                     /locus_tag="PLI16_03715"
     CDS             complement(1466..1948)
                     /locus_tag="PLI16_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83695.1"
                     /translation="MKKALYLILLLVFCTVQIQAQEVPEINKKIIDYVNTVIGKKVDY
                     GECWDLAYRAVTKNNCEWDGKYKWGQEIDPATDSVFPGDILQFYNVTLKYKDGNKIYK
                     EIFKLHTAIIYSVKGKGHYEIAHQNNAFSGRKVGLSDLDIRNKISGTIEFYRPVRKTN
                     "
     gene            2096..3574
                     /locus_tag="PLI16_03720"
     CDS             2096..3574
                     /locus_tag="PLI16_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH83696.1"
                     /translation="MANPIRKLAGQTAIYGVSTIVARLMNYLMVPIHTRIFAPPDYGI
                     VGEMYAYVSLLIVLLTYGMETAFFRFYEKNNPDRERVYNAANLSLIVSSTVFILLVFW
                     VAQPLADMIRYPQHSEYVRWFALIIAFDALSTIPFAKLRAINRPKMFAFVKIFNISVY
                     VALNLFFLLLCPYLGKNGLLTGLTNLVYNPSIGVGYIFIANLAASGITLVMLYIIVPI
                     SKFRMDTAVWRKMIRYALPLLIFGLAGIINETIDRILLKYLSPPDIAMSQVGIYSACY
                     KISIMMTIVIQAFKYAAEPFFFSQAKESNAKMLYADVMKYFVIVCSFIFLAIMMYIDV
                     VKYFVGKEYFEGLVVVPILLLANMFLGIFYNLSIWYKLTDKTRFGAYLSIMGAIITLV
                     LNYLLIPLIGYLGSAWATFFCYTSMMVASYFIGQKHYHVNYNIKRILLYIMVPVALYY
                     GSTYIVTDMQWVHWTVNTILLLSFVVMVIIIEKPKILNLIRK"
     gene            3574..4008
                     /gene="dut"
                     /locus_tag="PLI16_03725"
     CDS             3574..4008
                     /gene="dut"
                     /locus_tag="PLI16_03725"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOH83697.1"
                     /translation="MNVKIVNKSKHPLPKYETDASAGMDLRANLDSPVVLKPLQRVLV
                     PTGIFIEIPVGYEAQVRPRSGLAIKKGVTVLNTPGTIDADYRGEVMVILVNLSSEDFV
                     INDGERVAQMVFARHETASWIEVDVLQKSDRGAGGFGHTGDK"
     gene            4177..4413
                     /locus_tag="PLI16_03730"
     CDS             4177..4413
                     /locus_tag="PLI16_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH83698.1"
                     /translation="MSDITARVKAIIVDKLGVDENEVAPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDEEAEKIGTVGDAIKYIENNTK"
     gene            4425..5678
                     /gene="fabF"
                     /locus_tag="PLI16_03735"
     CDS             4425..5678
                     /gene="fabF"
                     /locus_tag="PLI16_03735"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOH83699.1"
                     /translation="MELRRVVVTGLGAITPIGNNLNEYWDALVRGVSGCASITRFDTS
                     KFKTKIACEVKNYDAGNYFDRKEAKKLDLYAQFALIAAQEAMADAAFDLTAINLNRAG
                     VIWGSGIGGLDTFTDEMLEYAKGDGTPRFNPFFIPKMIADIAGGNISIKYGFKGTNYS
                     TVAACASSTHAIIDAFNYIRMGKEDVMISGGSEAAIGIGGIGGFNAMHAISVRNDDPA
                     TASRPFDKDRDGFVLGEGAGTLVLEELNHALKRGTKIYCEIVGGGLSADAYHITQPHP
                     EGEGAYRVMLNALQDAGMQPQDIDHINTHGTSTPLGDIAESKAIVAAFGEHAYKMIVN
                     STKSMTGHLLGAAGAAESIACIMAINNGIIPPTINHFTDDPDVDNRINFTFNKAQNRE
                     INTALCNTFGFGGHNASLIFRKFNG"
     gene            5730..6398
                     /gene="rnc"
                     /locus_tag="PLI16_03740"
     CDS             5730..6398
                     /gene="rnc"
                     /locus_tag="PLI16_03740"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOH83700.1"
                     /translation="MLGFKPGNINLYQLAFLHRSAAIEVIGGHKNSNERLEYLGDAVL
                     SSIIADFLFRKYPLKDEGFLTEMRSKLVSRPQLNKLSQKLGIDKLITSEHDSGSVYKS
                     ISGDAFEALVGAIYLDKGYTFTTKIIINQIIKCLIDIDTLEKTELNYKSALLELAQKE
                     KKPLDFKVVQESGKGFNKQYVVEVSFDNKILAKGYGFSIKEAEQNGAESAFSILNSQE
                     NTKP"
     gene            6457..>7268
                     /locus_tag="PLI16_03745"
     CDS             6457..>7268
                     /locus_tag="PLI16_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOH83701.1"
                     /translation="MNKGKKTIERLLIWRLKHLSDRNFLMILSVFCGFISGIFAVFTK
                     NLVFFIESLTLFDKNPGQLHYEYFFYPIIGIWLTILFKKYVVKKQLIYGIPGVLRSIS
                     RESGYMSPKSIYSPAVASSITLGFGGSVGMEGPAVNMGGAIGSNVGRWLGLKYNHIVL
                     LMACGSSAAIAGLFKAPIAGVVLALEVLMIDLSLASLVPLLIAAVTGTLTSYFFLGQN
                     NIYSIAPFDPIDFSNLLFFAVLGIFCGFISVYFTRVLKFFDKHLNKIKNTYI"
BASE COUNT         1997 a         1556 c         1555 g         2160 t
ORIGIN      
        1 atgccggttt tatttttatc ggcctgatga atgtgggcgg caacccatac gtgattgaat
       61 ataacgtgcg catgggcgac cctgaaacag aaagcgtatt gccccgcgta aaaaacgacc
      121 tggtggaact gctgctggca acggcaagcg gaaccctgca tgaaaaacaa ctggacattg
      181 atgatcgcta tgccgttact gttatgatgg tgtcggaagg gtaccccgga aaatacaaaa
      241 aaggcaagaa gattgccggc ctgaacgccc tcgacggcag tactgtcttt catgccggaa
      301 caaccgtgga tgcagaagga aacataataa ccaacggagg ccgggtgctg ggagtgatgt
      361 catatggcgc cacgctgacg gaggcaaggg aaaaatcata caaaaacaca gctatcattg
      421 attttgaggg aaagtattac agacacgata tcggtaatga tttgatgtaa aaccggcaac
      481 actttgttta cacgcatttt taaatatcat accaattttc agatagtgtt tatcctgctg
      541 tctgccttgg cgttgtggct gccggcattc tttaaaggcg ttgacatgat gggcactatt
      601 gacctttcct atgggtacaa cacgctattt tttaaaacat acagcatccc attgctttat
      661 atggggttgg cttttatctt attgttggcc gaagccctgg tgctgaatac aatgcttgtc
      721 gagatcaaaa tttcgcccaa aaactcttac cttccggcgt ttttgtatat tttgctgatg
      781 agcagcagtc caaaattgct aacgctgcat ccttgtctta tcgtcaacgc ctttatcatg
      841 atcgtattaa tgctgctgtt tgagataaaa aataaagacg acggctaccg ggagattttt
      901 ttgtgcggca tactgacggg ccttggttcg ctgttctatt ttaaggctgt gggcttattg
      961 ataataattt ggatatttat cttgctttac cggcctttta cttggcggga atgggttatc
     1021 agcctgttgg gagttgccac ggtgtatgtg tatttgttta cttatttctt gcttacggat
     1081 caattgtcgg cggtattgtt tgattaccgg agggtttttt cctccatgag ctttggcggg
     1141 gatttccgca cgctttcctt atatcagtac ataaccttag cgctgatagt ggtcttattt
     1201 ttgatttcct cggtgaagat gtttgtgttg tcacacgaga aggtgattca cgtcagaaaa
     1261 gccctatatg ctatattctg gctacttgcc gttaacaccc tgagcgtgct gttccttgta
     1321 aaaaatctga tgtatgaatt ttcttacacc cttcttccgc tgtcaatact tgtcgcttat
     1381 tattacctga atctgaaaaa aaaactattc ggcgaaatta tcctcggcct tttcattgtt
     1441 tccataattc ttcagaaaat ctttatcagt tagtttttct gacaggcctg taaaactcta
     1501 tggtgcccga tattttattc cggatatcaa gatcgctcag ccccacctta cgaccagaaa
     1561 atgcattgtt ctggtgggca atttcatagt gtccttttcc ctttacgctg tatatgatgg
     1621 cggtgtgcag tttgaatatc tctttgtaaa ttttgttgcc atctttatat tttagtgtta
     1681 cgttataaaa ttgcagaatg tcgccgggaa aaacactgtc ggttgcagga tcaatttctt
     1741 ggccccactt gtattttccg tcccattcgc agttgttttt tgttaccgcc cggtacgcca
     1801 gatcccaaca ctcaccataa tcaacttttt tcccaatcac agtgttcacg taatcaataa
     1861 tttttttatt tatctccggt acctcctgcg cctgaatttg aacagtgcaa aataccaata
     1921 gcagtataag atataaagct tttttcatga cttgccagac cattattact aataacgtaa
     1981 gaatacctcg aaataatact tacaagccaa aaaaccggag ataaaataaa aaattgtatt
     2041 ttatgttttt atcactaatg agcattataa ttataaattt gcaaaaaaaa atagagtggc
     2101 caatccaatc agaaaattag ccggacagac agccatttat ggcgtaagca ccatagttgc
     2161 ccgcctgatg aattacctga tggtgcctat acatacccgc atatttgcac ctcccgatta
     2221 tggcatagtg ggcgaaatgt acgcttatgt ttcgttgctg atagtgttgc tcacctatgg
     2281 catggaaacg gcttttttca gattttacga aaaaaacaat cccgacaggg aaagagttta
     2341 taatgcagca aacctttcgc taatagttag ttccacggtt tttatcctgc tggtattctg
     2401 ggttgcccaa ccgctggccg atatgatcag gtatcctcaa cacagcgaat atgtccggtg
     2461 gtttgccctc atcatcgcct ttgatgccct gtccaccatt ccttttgcca aactcagggc
     2521 catcaaccgg ccaaaaatgt ttgcctttgt aaaaattttc aacatctcgg tttatgtggc
     2581 gctcaacctg ttctttttgc tgttatgccc ctacctgggt aaaaacggct tattaaccgg
     2641 cctgaccaat ctggtgtata acccctccat tggtgtcggg tacatattta ttgccaacct
     2701 ggctgccagt ggaattacct tagtaatgct ttatatcatt gttcccatca gcaaattccg
     2761 catggatacg gccgtctgga gaaaaatgat ccgttatgcc ctgcctctgc tgatatttgg
     2821 cctggctggg attataaacg aaaccatcga ccgcatactg cttaaatatt tgtcgccacc
     2881 cgacattgcc atgtcgcagg taggtattta ttcagcctgt tacaaaattt ccatcatgat
     2941 gaccattgtg attcaggctt ttaaatacgc cgccgaacct tttttctttt cacaggccaa
     3001 agaatccaat gccaaaatgt tgtacgccga tgtgatgaag tattttgtga ttgtttgttc
     3061 ctttattttt cttgccatca tgatgtatat cgacgtggtg aaatattttg tcgggaaaga
     3121 atactttgag gggttggtcg tggtacccat actattgtta gccaatatgt ttctgggaat
     3181 tttttataat ctttccatat ggtacaagct aaccgacaag acaaggtttg gcgcttatct
     3241 gtccatcatg ggggccatca taacactggt actgaattat ttgctaattc cacttattgg
     3301 ctatctgggc tctgcgtggg ccacattttt ctgctacacc tctatgatgg tggcctcgta
     3361 ctttatcggg caaaaacact atcatgttaa ttataatata aaaaggatac ttttatatat
     3421 tatggtgcct gttgcattat attacggcag tacttatatt gtaaccgata tgcaatgggt
     3481 gcattggact gtaaacacca tactgcttct aagctttgtt gtgatggtta ttattattga
     3541 aaagccaaaa atattaaacc taatcaggaa gtaatgaacg taaaaattgt caacaagtca
     3601 aaacatcctt tgcccaagta cgaaaccgat gcttccgcgg gaatggacct gagggcgaac
     3661 ctcgatagtc ctgttgtatt gaaacccttg cagcgggtgc ttgtgcctac cggcatattt
     3721 attgaaatcc ccgtgggcta tgaagcgcag gtacggccgc gcagcggact ggccataaaa
     3781 aaaggggtta ccgtacttaa cactcccggc accattgatg ccgactaccg aggagaagta
     3841 atggttattt tggtcaatct ttcctccgaa gattttgtga ttaatgatgg cgaaagggtg
     3901 gctcagatgg tatttgcccg gcacgaaaca gcctcctgga tcgaagtgga tgtgctgcag
     3961 aaatccgacc gcggagcagg cggttttggc catacgggcg ataaatagca aggcaaattc
     4021 tcttaaaatt tgttaaaata actctccgat aataatttat atgcgcctgt atttcagatt
     4081 attataataa aaaacaatct ctccaggcaa aaaaaatctt tgccatgtct gaattaattt
     4141 tttcctttgc aacttgttta ataaaaaatt ataattatgt cagacattac agcaagagta
     4201 aaagctatca tagttgataa gcttggcgta gacgaaaacg aagtagcccc cgaagcaagt
     4261 ttcaccaacg atctgggtgc agattctctt gacactgtag aattgatcat ggaatttgaa
     4321 aaagaattca acatagccat tcccgacgaa gaagctgaaa aaatcgggac agttggcgat
     4381 gccattaagt atatcgaaaa taatacgaag taagactttt tttaatggag ttacgtcggg
     4441 ttgttgttac cggacttggc gcaataaccc ctataggcaa caatctcaat gagtattggg
     4501 atgcgttggt caggggggtt agcggatgcg cgtcaattac acgctttgac acatcaaaat
     4561 ttaaaacaaa aattgcctgc gaagtaaaaa actacgacgc aggtaattat tttgatcgca
     4621 aagaagcgaa aaaacttgac ctgtatgcac aatttgcctt gatagctgca caggaggcaa
     4681 tggccgatgc tgctttcgac ctcacggcca ttaatttaaa cagggccgga gtaatctggg
     4741 gttcgggaat tggcggattg gatactttta ccgatgaaat gctggaatat gccaaaggag
     4801 acggcacacc gcgttttaat ccgtttttta ttcccaagat gatagccgat attgccggcg
     4861 gcaatatttc cataaaatac ggatttaaag gtaccaacta ttctacggtg gcagcttgtg
     4921 cttcatccac ccatgccatc atcgatgcct ttaactatat ccggatgggt aaagaagacg
     4981 tcatgatcag cggcggttcc gaagcagcca ttggcattgg cggtataggc ggctttaatg
     5041 ccatgcatgc catttcggtg cgcaacgacg atcctgcaac ggcctcacgt ccttttgaca
     5101 aagacagaga tggctttgta cttggcgaag gagctggcac attagttttg gaagaattaa
     5161 atcatgccct gaaaagaggc accaaaatct attgcgaaat agtgggcgga ggcttatccg
     5221 cagatgccta ccatatcacg cagccacatc ccgaaggcga gggcgcttac cgtgtaatgt
     5281 taaatgccct gcaggatgca ggcatgcaac cccaggacat tgaccatatc aatacccacg
     5341 gcacttccac ccctctgggc gatattgccg aatcaaaagc tattgtagcg gcctttggcg
     5401 agcatgctta caaaatgatt gtcaactcga caaaatccat gaccggacat ttgttgggtg
     5461 ctgccggcgc cgctgaatcc attgcatgca tcatggccat aaacaatggt attattcctc
     5521 cgaccatcaa ccactttacc gacgaccccg acgtggacaa ccgcattaat tttactttca
     5581 acaaagctca aaacagggaa ataaataccg cactgtgcaa tacctttggc tttggcggac
     5641 ataatgcttc attgattttc aggaaattta acggttaatc tcttctggct acgacaactg
     5701 ataaagaatt atccaaggca atacaaaata ttctcgggtt taagcccggc aatattaatc
     5761 tgtatcaact tgcttttttg caccggtcgg cagccatcga agtcattggt ggacacaaaa
     5821 actccaacga acgccttgaa tacctgggag atgccgtctt aagctccata atcgccgact
     5881 ttcttttcag aaaatatcct ctgaaagatg aaggttttct gaccgaaatg cgctcaaagc
     5941 tcgtcagcag acctcagttg aataaacttt cgcaaaaact cggcatcgac aaactgatta
     6001 cttcggaaca cgacagcggc agcgtgtaca agtcaatttc cggtgatgcg tttgaagccc
     6061 tggtaggcgc catatacctc gacaaagggt acacttttac aacaaagatt atcatcaacc
     6121 agatcatcaa atgcctgata gatattgaca ccctggaaaa aaccgaacta aactataaga
     6181 gcgccttgct tgaattggcc caaaaagaaa aaaaaccgct tgatttcaag gtggttcagg
     6241 aaagcggcaa gggttttaac aagcaatacg tcgtggaggt gtcttttgac aataaaatac
     6301 tggcgaaagg atatggtttc tctatcaaag aagccgagca aaacggagcc gaaagtgctt
     6361 tttccatcct gaattctcag gaaaatacaa aaccgtaact atttttctta ttgtaaaaaa
     6421 tgtatttttg cctttctttg tgaatgtatt ttttcaatga ataagggtaa aaaaacgata
     6481 gaacgcctgc ttatctggag attaaaacac ctcagcgatc gaaattttct tatgattttg
     6541 agtgtttttt gtggttttat ctccgggatt tttgccgtat ttaccaaaaa cctggtattt
     6601 tttatcgaaa gccttaccct ctttgataaa aatccggggc aacttcatta tgaatacttt
     6661 ttctacccga taattggcat ttggcttacc attttgttta agaagtatgt ggtaaaaaaa
     6721 cagttaattt acggcatccc cggagtgttg cggtccattt caagggaaag cgggtatatg
     6781 tcgccaaaaa gtatttattc gccggctgtt gccagttcca tcaccctggg ttttggcgga
     6841 tctgtgggta tggaaggccc tgcagtgaac atgggcggag ccattggttc caacgtgggg
     6901 cgctggcttg ggttgaaata taatcatatc gtgttgctca tggcttgcgg gtcatctgca
     6961 gccattgccg gcctgtttaa agctcccatc gcaggagtgg tgcttgctct cgaggtgcta
     7021 atgatcgacc tgtccctggc ctcgctggtg ccgctgctca tcgctgcggt aaccggaact
     7081 ctcacctcct atttttttct gggacaaaac aatatttata gcattgctcc ttttgatcct
     7141 attgattttt ccaacctgct gttttttgct gttctgggaa ttttttgcgg gtttatctcg
     7201 gtatatttta cccgggtatt gaaattcttt gataagcatt taaataaaat aaaaaacacc
     7261 tatatcag
//
LOCUS       DAOSFP010000084         7253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8931_length_7253_cov_3.702834, whole genome shotgun sequence.
ACCESSION   DAOSFP010000084 DAOSFP010000000
VERSION     DAOSFP010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8931_length_7253_cov_3.702834"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /locus_tag="PLI16_03750"
     CDS             <1..2070
                     /locus_tag="PLI16_03750"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOH83702.1"
                     /translation="RSPKSYNSQVGVPLSVWQMQPEHELAIFEAGISEPEEMKILQPI
                     ISPTIGIFTNIGTAHDKNFLNVRQKIGEKLNLFRRIETLIYCSDNYDIQERIIKSGIQ
                     PQKCFFSWGSKHKADLTIKSIEKNQNHCIISAVYKSENITVQIPFTDDASIENAIHCW
                     ALMLYLDYPHETIAARLALLTPVAMRLELKAGINNCSVINDSYSSDLNSLNIALDFMC
                     QQKQHSKKTVILSDILQSSLTDHELYGEIANLLVSKGVTRFIGIGPSISKNEKLFPME
                     KSFFIDTDAFLAKVPGSAFHNETILIKGARIYAFEQISRMLQQKSHETLLEINLNALI
                     HNLNFYQNKLKKGTKVMAMVKALSYGSGGYEIANVLQYHNIDYLAVAYADEGIELRKA
                     KITTPIMVMNPEEESFDLMKKFSLEPEIYNFRTLTLLIESITAYESKEPYKMPVHIKI
                     DTGMHRLGFLPDEIDKLLAVLKQNDSIIVRSVFTHLAASENNNEDEFSRQQIKTLETV
                     CARMKEEIKTPFMMHVLNSAGISRFPEAQFDMVRLGIGLYGISFSEEEQQYLQNVSSL
                     SSIISQIKTISARDTVGYGRKYIAGGDARIATIPIGYADGLNRKLGNGNGFMIVNGKK
                     APIIGSVCMDMCMLDITGLEAEEGDIVYVFDDAATIKEIADRLDTIPYEILTSVSSRV
                     KRVYYYE"
     gene            complement(2061..3149)
                     /locus_tag="PLI16_03755"
     CDS             complement(2061..3149)
                     /locus_tag="PLI16_03755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOH83703.1"
                     /translation="MKKILFIVAHRPGRSPGQRFRFEQYLDYLANKDYSYEFSFLLTE
                     NDDKVFYSKGKYFSKFLIFIKSILLRFRDLRRVRNYDIVFIYREAVMFGSTFFEKKFK
                     KKGALIILDFDDAIWLMDVSEGNKKLSWLKRPSKTADILKLSDMAFVGNSYLATYAQQ
                     YNPNVKIVPTTLDTRKMKTKHQMVSEKICIGWTGSHTTLKHFELAIPFLKKIAEKYPV
                     KIKIISDIPLNSNELRFDFCRWNKDSETEDLSEIDIGIMPLPDNDWARGKCGFKGLQY
                     MAVGIPAVMSPVGVNTEIIQDGENGFLAANEDEWIEKLSLLIESPELRKKIGATGRKT
                     VEEKYSFNAWKDTYVGYFEELLTKKNHS"
     gene            complement(3156..3851)
                     /gene="purQ"
                     /locus_tag="PLI16_03760"
     CDS             complement(3156..3851)
                     /gene="purQ"
                     /locus_tag="PLI16_03760"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOH83704.1"
                     /translation="MKFGVVIFPGSNCDHDLLYVLKKILKQEVVALWHKDKDLQKCDF
                     IFLPGGFSYGDYLRSGAIARFSPIMEKIIDYANNGGYLMGICNGFQILCEAQLLPGAL
                     LSNNNQQFICKNVFIKPVSQKAAPVKYLDPEKALKIPVAHAEGRYYADKKTIKQLYEH
                     DQILFKYCDAAGHITEDSNPNGTIDNIAGICNRTRNVFGMMPHPERACDKELSNTDGK
                     LIMDSLLKTLTKK"
     gene            complement(3925..4878)
                     /locus_tag="PLI16_03765"
     CDS             complement(3925..4878)
                     /locus_tag="PLI16_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83705.1"
                     /translation="MSNQDFQEALEQNDIDLLAKIPKSDLHNHAALGSRIEFLEKWAK
                     QKIIRPPQEMKKFSDFEDYLEKAFNKFVKAPKFFEYAKTATLEQARLDGVKVLQISLD
                     SRFFSGSDKDCREKIDIVQGAFRRISPGIYFVPQLGLQRNQDQKKLFYDAERLLDTGY
                     FRSIDLYGDELYGDVESYIPLYRKAKKQGMVLTAHAGEYGTAVSVKKAVELLELDQVQ
                     HGIAASESKEVMRWLADNKIILNVCPTSNVMLCRVPSMAKHPVRILYDHGVRVTINTD
                     DLMVFNQSVSQEYLNLYRAGVLGIGALDEIRQNGLHAFKGQ"
     gene            5299..6825
                     /gene="gltX"
                     /locus_tag="PLI16_03770"
     CDS             5299..6825
                     /gene="gltX"
                     /locus_tag="PLI16_03770"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOH83706.1"
                     /translation="MMTEKIRVRFAPSPTGPLHIGGVRTALYNYLFAKKHHGDMILRI
                     EDTDQTRFVPGAEEYIIESLKWCGINYNEGIEAGGPHMPYRQSERKAIYRQYAQQLVD
                     NGYAYYAFDSAEALDTLRKSHEAEKKTFQYDQKNRLQLNNSLTIPESKTIELLESGAP
                     YVIRIKIPENEKVTINDLIRGSVTFDSSLLDDKVIFKSDGLPTYHLANVVDDHLMEIS
                     HVIRGEEWLPSAPLHVLLYRYLGWENDMPYFAHLPLLLKPDGNGKLSKRDGDRLGFPV
                     FPLQWTDPKSGEISSGYRESGYIPDAFINMLALLGWNPGTEQEIFSMDQLIDAFSLER
                     VGKSGSKFSPEKAKWFNHQYIQNKNDEELAKLFGRELAGRNINADWASVTRIISLIKE
                     RITFTHELWAQSSYFFEAPVSYDAKSVKDRWKADSAEKIKEISQVINQCAIWTADEIK
                     QKIMDYINEKQYHTGQIMNALRVVIVGAAMGADLCTIIEIIGKDETLKRIEAGISKLG
                     "
     gene            6844..>7253
                     /locus_tag="PLI16_03775"
     CDS             6844..>7253
                     /locus_tag="PLI16_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOH83707.1"
                     /translation="MTNPSDWLARTELLIGPEKLERLRRAHVLVLGCGGVGSAAAEML
                     CRAGVGKLTIADADIVQPSNRNRQLIALLSTEGLKKTQVLADRLRDINPGIEVITVDK
                     FLKEDPMIELLDHPYDYIVDAIDSLSAKAYMLMYA"
BASE COUNT         2097 a         1668 c         1488 g         2000 t
ORIGIN      
        1 cgcagcccca aaagttacaa ctcacaggtc ggggtgccgt tatcggtatg gcaaatgcaa
       61 cccgaacacg aactggccat ttttgaagcg ggaatttcgg aaccggaaga aatgaaaatc
      121 ctgcaaccga ttattagtcc cactatcggt atctttacca atattggcac cgcccacgat
      181 aaaaactttc tgaatgtcag gcaaaaaatc ggtgaaaaac taaacctctt ccgacgcatc
      241 gaaaccctca tttattgttc ggataactat gatatccaag aacgtataat taaatccggc
      301 attcaacctc aaaaatgttt tttttcctgg ggctccaaac acaaagccga tttgaccata
      361 aaaagcattg aaaaaaatca aaaccattgc ataatctctg cggtttataa atcagaaaat
      421 atcacggtac aaattccctt taccgatgac gcctccatag aaaatgccat acattgctgg
      481 gcgttaatgc tttatctgga ctatcctcac gaaaccatcg ccgccagatt agccctgctg
      541 acacccgtgg caatgcgtct cgagctgaaa gccggcataa acaactgttc tgttattaac
      601 gacagctata gttcggacct caattcgctg aacatcgccc tggattttat gtgtcagcaa
      661 aaacagcaca gcaaaaaaac cgtgattttg tcggatatcc tgcaaagttc ccttacggat
      721 cacgaactct atggtgaaat agctaacctg ttggtatcta aaggggtaac gcgttttatc
      781 ggtatcgggc cttccatttc gaaaaacgaa aagcttttcc cgatggaaaa gtcatttttt
      841 attgacactg atgcctttct ggcaaaagtt cccgggtcgg cattccataa cgaaacaata
      901 cttatcaagg gcgcacgtat ctatgctttc gagcagataa gccgtatgtt gcagcaaaaa
      961 tcgcatgaaa cgcttctgga aattaaccta aacgctctta ttcataacct gaatttttac
     1021 cagaacaaac ttaaaaaagg caccaaagtc atggcgatgg taaaagccct ttcatacgga
     1081 agcggcggct acgaaattgc caacgtattg caataccaca acattgatta cctggctgtg
     1141 gcttacgctg atgaaggcat agaactccgc aaggctaaaa ttaccacccc tatcatggtc
     1201 atgaaccccg aagaagaaag ttttgacctg atgaaaaagt tcagtcttga accggaaata
     1261 tacaattttc gcacgctcac gctgctcatc gaatccatca ccgcctacga atcaaaagag
     1321 ccttacaaaa tgccggtgca cataaaaata gacaccggta tgcaccgcct gggtttttta
     1381 cctgatgaaa ttgacaagtt gttggctgtg cttaaacaaa acgatagtat tattgtccgg
     1441 tcggtgttta cgcacctggc ggccagcgaa aacaacaacg aggatgagtt ttcgcggcaa
     1501 caaataaaga cactcgaaac ggtttgtgca cgtatgaaag aagaaatcaa aaccccgttt
     1561 atgatgcacg tgttgaactc tgccggaatt tcccgctttc ctgaggcaca attcgatatg
     1621 gttaggttgg gaatagggct gtacggcatt tcattttcgg aagaagaaca acagtatctg
     1681 cagaatgtca gcagcctgag ttctatcatt tcgcagatta aaaccatttc ggcccgcgat
     1741 acggtaggtt acggacgaaa atacattgcc ggcggagacg cacgcattgc cactattccg
     1801 ataggttatg ccgatggcct caaccgcaag ctgggcaacg gcaacgggtt tatgatcgtg
     1861 aatggcaaaa aagcgcccat tatcggcagt gtgtgcatgg acatgtgtat gctcgacata
     1921 acaggccttg aagccgaaga aggcgatatt gtttatgttt tcgatgatgc tgccaccatt
     1981 aaagaaattg ccgaccgcct cgacactatc ccatacgaaa ttctcacgtc cgtttccagc
     2041 cgggtaaagc gtgtgtatta ctatgaatga tttttcttcg taagcaattc ttcaaaatac
     2101 ccgacgtagg tgtctttcca ggcattgaaa gaatattttt cttccactgt tttacgaccg
     2161 gtggcaccta ttttttttct cagttcgggc gattcaataa gcagggataa tttttcgatc
     2221 cattcatctt catttgccgc cagaaaacca ttttccccgt cttgaattat ctccgtattg
     2281 acaccaaccg gcgacataac cgccggaatc ccgaccgcca tatattgcaa acccttaaac
     2341 ccgcattttc ctcgggccca gtcgttatct ggcaagggca ttatcccaat atcaatttct
     2401 gaaaggtctt ccgtttcgga atccttattc cagcgacaaa aatcaaaacg taactcattg
     2461 gagttcagtg gtatgtcaga aatgattttt atttttacag gatacttttc cgctattttt
     2521 ttcaaaaacg ggatagctaa ttcaaaatgt tttaatgtgg tgtgcgaccc tgtccagcca
     2581 atacaaatct tctcggagac catctggtgt ttggttttca tttttcgggt atcaagggtg
     2641 gtagggacaa ttttcacatt gggattatac tgttgggcat aggtggctaa ataagaattt
     2701 ccaacaaaag ccatatcgga aagtttcaaa atatcagctg tttttgaagg ccttttaagc
     2761 caactcagct ttttgttgcc ttccgacaca tccataagcc agatggcatc atcaaaatca
     2821 aggataataa gggcgccttt ttttttaaat tttttttcaa aaaaagtact gccaaacatc
     2881 acggcttccc ggtaaataaa tacgatatcg tagttccgca cccgcctcaa atccctgaat
     2941 ctcaataaaa tgctttttat aaaaatcaga aatttgctaa agtattttcc cttgctgtaa
     3001 aaaaccttat cgtcattttc ggtaagcaga aaagaaaatt cgtaagaata atccttgttc
     3061 gcgaggtaat caagatattg ttcaaaacga aacctttgcc ctggcgaccg gcccggccgg
     3121 tgagcaacga taaacaaaat ctttttcaaa acaacttatt tttttgtcag ggttttcaac
     3181 agcgaatcca taatcagttt gccgtcggta ttggaaagct ctttgtcgca agccctttcg
     3241 gggtgaggca tcatcccaaa aacattcctg gtacggttgc agatacccgc aatattatca
     3301 attgtgccat taggatttga atcctcggta atatgtccgg cagcatcgca atatttaaaa
     3361 agtatctggt cgtgttcgta aagctgcttt atggtttttt tatcggcata gtaccggccc
     3421 tcggcatgtg ccacgggtat tttcagcgct ttttccggat caaggtattt gaccggtgca
     3481 gctttttgcg aaaccggttt gataaaaaca tttttacaga taaactgctg gttgttattg
     3541 ctcagcaagg cccccggcag caattgcgct tcacacaaaa tctggaaacc attacaaatc
     3601 cccatcagat aacctccatt attggcataa tcaattattt tttccataat gggcgaaaaa
     3661 cgcgcaatgg ctcctgaacg caggtaatca ccataagaaa atcctcccgg aagaaaaata
     3721 aaatcacatt tttgcaggtc tttatcttta tgccacaggg caacaacctc ctgcttaagg
     3781 atttttttta atacatacaa caagtcatga tcacaattag atcccgggaa tattacgaca
     3841 ccaaatttca ttttttttac ttttgttaag caaagatata ccctttaatt aaatttcggc
     3901 cttgttgaca aaaaaaactg tgcattattg tcctttaaaa gcatgtaggc cgttctgccg
     3961 gatttcatcc agcgccccga tacccaatac tccggcccta taaaggttga gatattcctg
     4021 acttacggat tgattgaaca ccatcaaatc atcagtatta atggtaaccc ttaccccgtg
     4081 atcataaagt atgcgaacag gatgttttgc catagagggc acccggcaca acattacatt
     4141 gctggtaggg cacacattga ggatgatttt attgtcggca agccagcgca tcacttcttt
     4201 cgattccgat gcggcaatac cgtgctgcac ctgatcgagt tccagtaact caacggcttt
     4261 tttcaccgag acagccgttc catactcacc agcatgagct gtgagaacca ttccttgttt
     4321 tttggctttc cggtagagtg gaatataact ttctacatcg ccatacaatt catcgccata
     4381 taaatctatt gaccggaaat agcctgtgtc aagaagtctc tcggcatcat aaaaaagttt
     4441 tttctggtcc tgatttcgtt gaagaccaag ctgcggtaca aaatatatcc cgggagatat
     4501 ccgcctgaaa gcgccttgaa caatatcaat tttttcccgg caatctttgt ctgaaccact
     4561 aaaaaaacgc gagtcgaggc taatttgaag cacctttacg ccatcgaggc gggcctgttc
     4621 aagggtggcc gtttttgcgt actcaaaaaa cttcggggcc ttaacaaatt tattaaatgc
     4681 tttttccaaa taatcctcaa aatcagaaaa ttttttcatt tcctgcggtg gcctgataat
     4741 tttttgtttt gcccatttct ccagaaattc tatgcggctt ccaagtgctg cgtggttatg
     4801 gaggtcgctc tttggaattt tggccagcag atcaatatcg ttttgctcta gtgcttcttg
     4861 aaaatcctga ttcgacattt ccgttatttt tttaagccaa aattaaaaaa aaagccaccc
     4921 cgttaagagt ggcttggttt aaaaatttgg cttgctcttg gtttgcggct acattttgta
     4981 ctcaatcagc atcattactg caaaaatcgc aagcatccat gcagcaaaga tgatccacag
     5041 tacgtatggt tttgctgttt tcatagttaa attattttta gtgtttgaca tcgcttgctc
     5101 ctttcctgtt tgttttttta tgtaacgtaa aattataccc cggcactggg ttaataccca
     5161 ctaaatcagt aaacggtatc aattttcaag tttacggcca taccggatgt tttatcgttt
     5221 ttacaacccg tggagaactt aatgacagtt cacatggggc ttgttaacct aaaatacgat
     5281 atttttgcaa aaatttttat gatgacagaa aaaatcaggg ttcgctttgc gcccagcccc
     5341 accgggcctc ttcacatagg aggcgttcgc acagctttat acaactacct ttttgcaaaa
     5401 aaacaccacg gcgacatgat actccgtatc gaagacacag accaaacccg ttttgttccc
     5461 ggggcggaag agtacatcat cgaatcgctg aaatggtgcg gaataaatta taatgaaggc
     5521 attgaggctg gcggcccgca catgccctac cgccaatccg aacgcaaagc catttaccgg
     5581 caatatgctc agcaattagt tgataacggc tatgcttatt atgcttttga ttcggctgaa
     5641 gccctggaca ccttaagaaa aagccacgaa gccgaaaaaa aaacctttca gtacgaccaa
     5701 aaaaaccgtt tgcaattaaa caactcacta actattcccg aatcaaagac catagaattg
     5761 cttgagtccg gtgctcctta tgtgatacgg ataaaaattc ccgaaaacga aaaagttaca
     5821 atcaacgacc tgattcgcgg ctctgtgact tttgattcct ctttgcttga cgacaaggtt
     5881 attttcaagt ccgacggctt gcccacctat cacctggcca atgtggtaga cgaccacctg
     5941 atggaaatat ctcatgtgat acgcggcgaa gagtggctgc catcggctcc cctgcacgta
     6001 ttactatacc gttatctcgg ctgggaaaac gacatgccgt attttgccca tttgccgctg
     6061 cttttgaaac ccgacggcaa tggcaaactc agcaagcgcg atggcgaccg gctgggattc
     6121 ccggtatttc ctttgcaatg gaccgatccc aaaagcggag aaatctcgag tggctaccgc
     6181 gagtccggat atatccctga cgctttcatt aatatgctgg ctctgcttgg ctggaacccc
     6241 ggaaccgaac aggaaatttt ttcgatggac cagcttatcg atgctttttc gctggaacgt
     6301 gttggaaaat ccggctcaaa atttagtcct gaaaaagcca aatggttcaa ccaccagtac
     6361 atacaaaaca aaaatgatga agaactcgcg aaactgtttg gccgggaact ggctggccga
     6421 aatattaacg ccgattgggc ttcggtgacg agaattattt cgctgataaa agaacgtatt
     6481 acctttaccc acgaattatg ggcccaatca tcctattttt tcgaagcgcc ggtaagctat
     6541 gatgccaaat ccgttaaaga ccggtggaaa gcagattctg ctgaaaaaat caaggaaatt
     6601 tcgcaggtaa tcaaccaatg tgctatatgg actgccgacg aaattaagca gaaaataatg
     6661 gactacatca atgaaaaaca gtatcatacc ggacaaatta tgaatgcgct ccgggtggtt
     6721 attgtaggag cagccatggg ggctgatctg tgtaccatta ttgaaattat tggaaaggat
     6781 gaaaccttga agcgtattga agcgggtatt tctaaacttg ggtaaatcct ttcgttacaa
     6841 caaatgacaa atccttcaga ctggctggct cgcaccgagc tgctgatagg ccccgaaaag
     6901 ctcgaaaggc ttcgcagggc gcatgtcctg gtgctgggct gcggtggtgt gggcagcgct
     6961 gccgccgaaa tgctctgccg tgcgggcgtt ggaaaactca ccattgccga tgccgatatt
     7021 gtacagccct cgaaccgtaa ccggcaacta atagcgctgc ttagcaccga ggggctcaaa
     7081 aaaacacagg tgttggctga tcgtctgcgc gatattaacc ccggaataga agtgataacg
     7141 gtggataaat ttctgaaaga agaccccatg atcgagcttc ttgaccatcc ttacgattat
     7201 attgttgatg ctatcgactc gctgtcggca aaagcatata tgctgatgta tgc
//
LOCUS       DAOSFP010000085         7251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_8934_length_7251_cov_4.121595, whole genome shotgun sequence.
ACCESSION   DAOSFP010000085 DAOSFP010000000
VERSION     DAOSFP010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8934_length_7251_cov_4.121595"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="PLI16_03780"
     CDS             <1..1467
                     /locus_tag="PLI16_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH83708.1"
                     /translation="AAIASFLLQKGVDVVSSESLLLSSSPKVNFIVSLLRYVDNPADE
                     LAAAYILNFLQHNYPADYDLNALIAQYQSVKSAGKSKYPLNEIFAIFQKDIECVKMSG
                     TPLFDLVESLVRTFGFHHQSDPFMVYFLDAISEFVSTKTPCLPDFIAWWDEKGATRSI
                     VVPEQLNAVKIMTIHKAKGLEFPVVIYPFADEMPGSKGASAWVMNSFDYCPEIPAFPI
                     AISAKGLEGSSFSDYFEQEQSLARLDMMNICYVALTRPEEQLYVLTKKYKENGHSFGS
                     LLKKYLMHRELWSEENDVYEFGSIAAVTTKEKHTSKGQTVVPENILSSDWAARMLIRH
                     DSTVLWADDETATTRGWGNIIHKILSEIKQAEDAIGVTTGMLLEGIISQADAEKITKY
                     IEQLMANPHFKSFFEPGLPVLTEAEILTPGNKVYRPDRILFEKDKNVVVDFKTGVQKD
                     DHKTQIDNYAAILTEINNKPTEKFLIYLHEEPLIIKVD"
     gene            complement(1929..2540)
                     /locus_tag="PLI16_03785"
     CDS             complement(1929..2540)
                     /locus_tag="PLI16_03785"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOH83709.1"
                     /translation="MFTGIVETTGKVVSIERDRTNIHITIETPIAKELKIDQSMCHDG
                     VCLTVVSVDYDKSQYVVTAIQETLDKTNLGHWQTGYEVNLERSMRMEARFDGHIVQGH
                     VDQTAVCNRVEEMDGSWKFWFDYDASKKNITVEKGSISVNGVSLTVVDSQPGQFSVAI
                     IPFTYQVTNFHNFKKGTVINIEFDVFGKYVAKLLQQYLDQKGL"
     gene            2639..3073
                     /locus_tag="PLI16_03790"
     CDS             2639..3073
                     /locus_tag="PLI16_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HOH83710.1"
                     /translation="MKFLSSQFYLILTATVILVVFPLSCKTTSGKENKPAAVAVKFLK
                     HLRAFEFDEARKLGTESTGKMLDMFSMMLELSKEKGKDPSKDKKDVNINVVKTAIDGK
                     NAVVTYKDENGKEQQLDLVKVKGKWLVNMKKETPALSPSGKN"
     gene            complement(3287..5344)
                     /locus_tag="PLI16_03795"
     CDS             complement(3287..5344)
                     /locus_tag="PLI16_03795"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HOH83711.1"
                     /translation="MKKLFVTICFVWFCTIIIFGQNTWSRLNPAVPQNDPLQSVFFVD
                     SNTGYSVGLGGIILKTVNGGGVWKVLTSGISDDLNSVYFTAADTGYAVGQSGRILKTS
                     DGGDSWIAQISGISNNLFSAYFIDDTTGYISGSGGKILKTTNGGTTWTIMTSGTTNDL
                     RSIYFPATDTGYAVGTYGIILKTNNGGLNWAAQTSGITVNLNSVCFADADTGYAVGYS
                     RMFLKTSDGGITWTYQQLSEDLLSVFFINADTGYVAGEAGTILKTTNAGDTWTLKTTN
                     TSYDLYAVYFTGISTGYAVGNNGIIKKTTDGGNSWNSQTVERDIFLRSVCFPVSDTGY
                     TVGNYGVILKTTDGGATWSTQTSGTTTDLNSVYFIDADTGYAVGNLVGFDGMAIILKT
                     IDGGMNWSIQPCLSAANLYSVYFVNSSIGYVVGHTGRILKTEDGGATWTLQTSGTQFV
                     LYSVYFVDSDTGFAVGDYGVSLKTTNGGELWTGQTITAVSSFSGVYFTDAHTGYAVGT
                     DGAIMKTTDGGFNWAFQTTNTTSNLHAVCFPDENTGYAVGAQGIILKTTDGGINWISQ
                     LHNSEYINNLWSASFPNAFIGYAVGDYEAIIKSTDISYGISSIITNDRIHIYPNPANH
                     FLFIENDTHHTTVSIYDLSGRRLLNRQITGNQIDVGNLPQGTYLLKITTPKGSATAKF
                     VKE"
     gene            5673..6329
                     /locus_tag="PLI16_03800"
     CDS             5673..6329
                     /locus_tag="PLI16_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOH83712.1"
                     /translation="MGNDLQSKERKTMWVVLLTAVTMIFEITFGLITKSMALLADGIH
                     MGSHVLAIGLSLGAYIAVRRLEKKDTFTGNSKKILSLSGYSSGLMLLIFAIFILVEAL
                     KRFFNPVEIQFKEAIIVAIAGLFINLISALILHHDHTHSDHNIKAAYFHVLADALTSI
                     IAIIALFGAMLWQIYWLDAAGAFIGSFVIIKWSAGLLKESGKVLLDIHAENDNADNNN
                     "
     gene            6593..>7251
                     /locus_tag="PLI16_03805"
     CDS             6593..>7251
                     /locus_tag="PLI16_03805"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOH83713.1"
                     /translation="MKKLLIPFLLLTGFCVLFGNHRTTAQNSWNTAAVFNGSCYHKEM
                     VTALNSTSGEMTVEFWIKLANYASNYAIIGKDQFRIMTGAKKIRIQVDGSTLLYSNAE
                     LDTGRWTHVAVTFSNTNNQIKVYLNGSLDNTLSTFTGAFTATSDTLFVGANVYVDTLQ
                     GELDEVRIWKTVRSASEIQQYYKIHLGWYGLTYYDNNLVYCQTYDFDVYSPGFYLPHG
                     GS"
BASE COUNT         2173 a         1591 c         1441 g         2046 t
ORIGIN      
        1 gccgctattg cctccttttt gcttcaaaag ggcgttgacg tggtatcaag cgaatcgctg
       61 ctgctgagtt cttcacccaa agtcaacttt atcgtatcgc tactacggta tgtcgacaac
      121 cctgccgatg agctggcggc cgcttatatc cttaatttcc tgcagcacaa ttaccctgca
      181 gactatgacc tgaacgccct gattgctcaa tatcagtcgg taaaatcagc cggtaagagc
      241 aagtatcctc tgaacgaaat ttttgccatt tttcagaaag atatcgaatg tgtcaagatg
      301 tccggcaccc cgctattcga cctggtggag tcgctggtca ggacattcgg gtttcatcac
      361 cagtccgatc cttttatggt ttatttcctg gatgctatca gcgaatttgt gagcacaaaa
      421 actccctgcc tgcccgattt tatagcatgg tgggacgaga aaggcgcaac gcgcagcatt
      481 gtagtgcccg agcaactcaa tgctgtaaag attatgacca ttcacaaagc caaaggactc
      541 gaatttcctg tggttatcta cccgtttgcc gatgaaatgc ccggcagcaa aggcgccagc
      601 gcatgggtga tgaactcttt tgactattgt cctgaaattc ccgcattccc aatagccatc
      661 agcgcaaaag gattagaagg atcttcattt tcggattatt ttgaacaaga acagagtctg
      721 gcacgcctcg atatgatgaa catttgttat gtagcgctca cacgtcctga agaacagctc
      781 tatgtgctca caaaaaagta caaagaaaat ggtcacagtt ttggctcact gctgaaaaaa
      841 tatttgatgc atcgtgagct gtggtccgaa gaaaatgatg tgtatgagtt tggaagcatt
      901 gcggctgtta ccacaaaaga gaagcacacc agcaaaggac aaacagtggt tccggaaaat
      961 atcctgtcct ccgattgggc cgccaggatg cttatacgcc acgattcgac cgtattatgg
     1021 gccgatgatg aaacggccac aacccgcggc tggggtaaca tcattcataa aatcctgtcg
     1081 gagatcaaac aagccgagga tgccattggt gttaccaccg gcatgcttct ggaaggcatc
     1141 atcagccagg ccgatgctga aaagataacg aaatacattg aacagctaat ggccaacccg
     1201 cattttaaga gctttttcga acccggacta ccggtgctca ccgaggcaga gatacttacc
     1261 ccgggcaata aggtgtaccg tcccgacagg atcctgtttg aaaaagacaa aaatgtggtg
     1321 gtggacttta aaaccggcgt ccaaaaagac gatcacaaaa cacaaattga taattacgcg
     1381 gccatattaa ccgagatcaa caacaaacct accgaaaaat tcctgatcta tttgcacgaa
     1441 gagcctttga taataaaggt ggactaaaag agcatgttca tcaattcaaa tatcgaacac
     1501 cgatcaacga agaatgattt aagaagtttc ttcttcaaaa atcgatattc ttcaatcgat
     1561 attcgtacat cattaaattc cttttaatat cgaacattga ttaacgaatg atgatttaag
     1621 agtggcatat cacttcaaaa atctaaaatc cctgcttgcc gtaggcaatg ctaatcgaaa
     1681 ttcatatatc acttaatccc ttttaagatc gaacaccgat taacgattga tgattccagt
     1741 acttcaaaaa tcgaaactcg tcaatcgaaa ttcatacatc actgaattcc ttttaatatc
     1801 gaacatcgat caacggatga tgatttacaa agtggccctt cacttcaaaa atctatccga
     1861 cctgctgccc gcagtgtcca cacagtttca ttaatttcaa tcgtcattta caaacaggca
     1921 aggctagatt acaggccttt ctgatcaaga tattgttgca aaagtttggc cacgtatttt
     1981 ccaaacacat caaactcaat attgataaca gttccttttt tgaaattatg aaaatttgtt
     2041 acctggtaag taaaagggat gatagctacc gagaactgcc cgggctgcga atcaaccacg
     2101 gtgagactca cgccgttcac agaaatggac cctttttcca ctgttatatt tttctttgac
     2161 gcatcgtagt cgaaccagaa tttccagctc ccatccattt cttcaacacg gttgcatact
     2221 gccgtctggt caacatgccc ctgcacgata tgtccgtcga aacgagcttc cattctcatg
     2281 ctgcgctcaa ggtttacttc gtaacccgtc tgccagtggc caaggttggt cttatccagc
     2341 gtttcctgta tggcggtaac cacatactgt gatttgtcgt agtccacgct taccacggtc
     2401 aggcacaccc cgtcgtggca catgctttgg tctatcttaa gctctttggc aataggggtt
     2461 tcaatggtaa tatgaatatt ggtgcggtcg cgttcgatgc tgacgacttt gccggtggtt
     2521 tcgactattc ctgtaaacat tgtttttggt ttttattgtt tcctgcaaag gtagcaaaaa
     2581 agaagatttc atatcaggat gggataattt gcctattttt gaaacaaata tttcgaaaat
     2641 gaaatttctc tcatcacaat tttatcttat actcactgct accgtgattt tggtagtgtt
     2701 tccgctatcg tgcaaaacaa cttccggaaa agaaaataaa ccggccgctg tggcagtgaa
     2761 atttctgaaa cacctcaggg cttttgaatt cgacgaagcc cgcaaacttg gcacggaaag
     2821 caccggaaag atgctcgata tgttttcgat gatgctcgaa ctaagtaaag agaaaggcaa
     2881 agacccctca aaagataaaa aagacgtgaa tattaatgtg gtaaaaacag ccattgatgg
     2941 gaaaaatgcc gtcgttacat acaaggatga aaatggcaag gagcagcagc ttgatttggt
     3001 aaaggtaaaa ggcaaatggc ttgtaaatat gaaaaaggaa acgccggcct tatctccttc
     3061 cggcaaaaat tgatgtttta gctcaatccc ttgttgagtt tgcatgatag gtataatctc
     3121 tatttgcctt agcttaaagt tttgtatcag aaaaaaatat taaaacgcaa aggaaatgcg
     3181 gtttgtgttt aagtgatatt atgattggac aaggaatgaa attccgtgac agcggggatt
     3241 cggaaacttc tcacattcaa gcatcaattc cgtgcgcgat aatacgctat tctttaacaa
     3301 atttagcggt tgcagatcct tttggagtgg taattttaag aaggtacgta ccttgtggta
     3361 aattgccaac gtcaatttga ttaccggtaa tttgcctgtt gagtagccgc ctcccgctta
     3421 aatcatatat agaaacagtg gtatggtgag tatcattttc tataaacaaa aaatggtttg
     3481 ccggattcgg ataaatatga atacgatcgt tagtaataat agaggatata ccatatgaaa
     3541 tgtcggttga tttaataatc gcttcataat caccaacagc atagccaata aatgcattag
     3601 gaaagctggc cgaccataga ttattaatat attcactatt atgaagttga gaaatccaat
     3661 ttatgccgcc atctgttgtt ttaagaatta taccctgagc acctacagca tagccggtat
     3721 tttcatcagg gaaacagaca gcatgtaaat tcgatgttgt attcgttgtc tgaaacgccc
     3781 agttaaaacc gccatctgtt gttttcatta tggctccgtc tgtcccaaca gcataacctg
     3841 tatgggcatc cgtaaaatat actccggaaa aagagctgac tgctgtgata gtctgaccag
     3901 tccataattc accgccatta gttgttttca ggctcacacc ataatcacca actgcaaagc
     3961 ctgtatcaga atccacaaaa tataccgaat ataaaacaaa ttgcgtcccg cttgtctgaa
     4021 gtgtccaggt agcaccgcca tcttcagttt tcagtatacg tcctgtatgg ccgacaacgt
     4081 atccaatgct cgaattaaca aaatagacag aatataaatt tgctgccgac aggcatggtt
     4141 ggattgacca gttcatccct ccatctatcg ttttcaggat gatcgccatg ccatcaaaac
     4201 ccactaaatt acctactgca taaccagtat cagcgtcgat gaaatacact gaatttaaat
     4261 ccgtcgttgt tccgcttgtt tgagttgacc atgttgctcc tccatcggtt gttttaagga
     4321 tcaccccata attacccacc gtataaccgg tgtcagatac cgggaaacaa actgaccgta
     4381 aaaaaatatc tcgttctacc gtctgtgaat tccagctatt gccgccatcc gttgtttttt
     4441 ttataattcc attgttacca acggcataac cggtgcttat gccggtgaaa tatacagcat
     4501 ataaatcata agaggtatta gttgttttca acgtccaggt gtcacccgcg ttggtcgttt
     4561 tcagtattgt ccctgcttct cctgccacat aacctgtatc cgcattgata aaaaatactg
     4621 ataataaatc ttcagataat tgctgatatg tccaggttat cccgccatcg cttgttttta
     4681 aaaacatacg gctatagcca acagcatagc cggtatccgc atccgcgaag cacaccgaat
     4741 taagattcac agtaatgcca ctcgtttgag cggcccagtt caagccacca ttatttgttt
     4801 tcaggattat gccgtaagta ccgacagcat agcccgtatc agttgccgga aaataaattg
     4861 acctcaggtc attcgttgtt ccgctggtca taattgtcca agttgtacct ccatttgttg
     4921 ttttcaggat ctttccgcca ctgccggaga tatatcctgt tgtatcatct atgaaataag
     4981 ccgaaaataa attattagat attccgctta tctgggcgat ccagctatca cccccatcgg
     5041 aagtttttaa aatccttccc gattgaccga cagcatagcc ggtatcagcg gcagtaaaat
     5101 aaaccgaatt taaatcatcc gatatcccac tcgttagaac tttccatact ccgccgccat
     5161 taactgtttt taatataata ccccctaacc ccacagagta gcccgtattg ctatctacaa
     5221 aaaacacgga ctgtaaagga tcattttgag gcactgccgg atttagccgc gaccatgtgt
     5281 tttgcccaaa aataatgata gtgcaaaacc aaacaaaaca aatagtaaca aacaattttt
     5341 tcatttttca ttaattgaaa atacttaata atattttata gcaaaaatat gtcaaaataa
     5401 taatccaagc aaaaaaaaac cgcattttaa tttgcacgtt agcaaagcgc acatagatgc
     5461 attccaaaat tctgcgcttc gacaaaatca ccataaatga tgatgcggca gggcaatgag
     5521 ttagcaggct cttttttgtt aacgattgta tttaaaaaaa aaccaaggct ttatacaatc
     5581 aaaaattgca ataccttagc cgcctgaaat cagcaaatgc aattcagagg acaaatagca
     5641 gagaactaat tgtatttcaa attataaaac atatgggtaa cgatcttcag tctaaagaaa
     5701 gaaaaaccat gtgggtagtt ttgctgacag cggttacaat gattttcgag ataacctttg
     5761 gccttatcac aaaatcaatg gcgttgctgg ctgatggtat tcacatgggg tcgcatgtgc
     5821 tggccattgg cttaagcctt ggagcttata ttgcagtcag gcggcttgaa aagaaagaca
     5881 cttttacggg taattcaaaa aaaatacttt cactttcagg ttatagcagc ggattaatgc
     5941 tgctgatctt tgcgatattt atcctggtgg aggcactgaa gcgttttttt aaccccgtag
     6001 aaatccagtt taaagaggcc attattgtgg caatagcagg cttattcatc aatttgatca
     6061 gtgcccttat tctgcaccac gaccatacgc attccgatca caacatcaaa gccgcctact
     6121 ttcacgtgct ggctgatgcc ctcaccagca taatagccat aattgcgtta ttcggagcca
     6181 tgctatggca aatttactgg ctggacgccg caggcgcttt catcggttcc tttgtaatta
     6241 tcaaatggtc agcaggacta ctaaaagagt cggggaaagt tttgctggat atccatgctg
     6301 aaaacgataa cgctgacaac aacaattaat gagtaagtct cgcattattc acgctttttc
     6361 agaatgtggt ttttcttgat cattgcaggt gcttttagtg ctattgttcg gaaatagcaa
     6421 cgtgctgcca gtcaaaaccg cgggcggtga aaatcagcaa aggcatgatt ttttccgtcc
     6481 tgctgagagc gctcgcaagc atacataact ttaattatta acaatttctt gatttttctc
     6541 cctcaatttc gtatcttcgc aataacaata caaccccata aaaatatata taatgaaaaa
     6601 attacttata ccatttttat tgctgacagg tttttgtgtt ttgtttggca atcaccggac
     6661 cacggcccaa aatagctgga acactgcggc tgttttcaac ggcagttgtt atcacaaaga
     6721 aatggtcact gcgctcaaca gcacttcggg agagatgaca gtggagtttt ggattaaact
     6781 ggcaaattac gcctcgaatt atgcaatcat tggaaaggac caattcagaa taatgacggg
     6841 ggcgaaaaaa atccgcatcc aggtggatgg atctaccctc ctctattcaa acgccgagtt
     6901 ggatacaggc aggtggacac atgttgctgt tacgtttagc aataccaaca accaaatcaa
     6961 ggtgtattta aacggaagtc tggataatac tttatcgact tttaccggcg ctttcactgc
     7021 tacttccgac acgctgtttg ttggtgctaa cgtctatgta gataccctgc aaggtgagct
     7081 ggacgaagtc agaatatgga aaacggttcg ttcggccagc gaaattcaac aatactacaa
     7141 aatacatctg ggctggtatg gattaaccta ttatgataac aacctggttt attgtcagac
     7201 ctatgatttt gacgtgtaca gccccggatt ttatttaccg catggaggct c
//
LOCUS       DAOSFP010000086         7023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_9337_length_7023_cov_3.810706, whole genome shotgun sequence.
ACCESSION   DAOSFP010000086 DAOSFP010000000
VERSION     DAOSFP010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9337_length_7023_cov_3.810706"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /locus_tag="PLI16_03810"
     CDS             complement(<1..1231)
                     /locus_tag="PLI16_03810"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HOH83714.1"
                     /translation="MSHPKDSISSGDLRFVENKGQWDPQIKYKADLDGGYLQCLQNKI
                     QFVFRDQAKLDWLLSYKLRAKNQHLVVNPDEFIINYHAYAASFINANPYTEIIPAGCG
                     SDYTNYYLGNDKSKWASRVRNYKMLTYKNLYDNIDMEFFEEKFLLKYQFTVHQGANPN
                     KILIEYQGATDISIQQSNLIIKTTVNQVTELAPVAFQIIDGEKVSVPCKFALQRNRVS
                     FKFPEGYNKNYDLIIDPVLIFSTYTGSLADNWGFTATYDEFGNTYSGGICFNDITNGY
                     PVSPGAFQVNFAGGEGTYNGGCDIALIKYDATGQNRLFATYLGGDRNDLPHSLIVDIN
                     NNLLVFGTTGSANFPIAGSAFDPTFNGGTDITYDYVLTFNLGVDMYVSKLKSDGTQLY
                     ASTFLGGTQNDGNNFFAP"
     gene            complement(1420..2175)
                     /locus_tag="PLI16_03815"
     CDS             complement(1420..2175)
                     /locus_tag="PLI16_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HOH83715.1"
                     /translation="MDTIDTIFNHRTIRKYKRNNIPEDLLTGILQAGTRASTTGNMQV
                     YSIIVTRDEEKKKKLCEYHFNQKMVLEAPVLLTFCADYHRFNQWCRQRNAEPGYDNFL
                     SFFTAAIDALLAAQNVALAAEANGLGICYLGTTTYNADKLIEFFGCPDGVVPVTTLVL
                     GYPDEEPGLTDRLPLEAIVHNEQYETYTPEKIDKLYKEKEELPLTKSLLIENQLETLA
                     QIFTRKRYTKNDNIHFSKSLLDVIHKQGFMNNE"
     gene            complement(2180..2869)
                     /locus_tag="PLI16_03820"
     CDS             complement(2180..2869)
                     /locus_tag="PLI16_03820"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH83716.1"
                     /translation="MKETQDILHFLQEHSNPSNVKGMARFGIATGQAFGVPLPALRQK
                     AKSYKKNRALALDLWQSGHHEARIMATMIDDCKQVTEAQMEQWAHDFDSWDVCDQCCS
                     NLFDKTAFAINKSTEWTTRHEEFVKRAGFTMIACLAVHAKKMDDRQFIEFLPLIVRES
                     TDDRNFVRKAVNWALRQIGKRNTALYPEALAVAQKLMLSENKTARWIGTDASKELQNE
                     KIINRIKSKEK"
     gene            complement(2880..3851)
                     /locus_tag="PLI16_03825"
     CDS             complement(2880..3851)
                     /locus_tag="PLI16_03825"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH83717.1"
                     /translation="MKYPTAFFFILVISTGFLFSQPADFTCKSYDTLNSDIPDIVIKT
                     VAIDNDDNIWFATGDCFYGGLLVKYDGARYTVFDSSNSGLNDRFVTSILISRNGDKWV
                     GSRFHGLYKYDDREWINYTPANCPLPNPHITDLAEDRQDVLWVASEHGLASHDGKNWN
                     VFTAENSGLPSNTVNAVCVDAHNNKWVATANGFAKFDGRDWQVFNTANSQLPNNQVNS
                     IYTDQSGGVWVVYWSGIIKINDTGWTVYNHENSGLPQFCDYKVTGDKNNVFWLATNTG
                     LIRWGETGWKLYTPLNSCVLSFFVNKVAIDSKNRKWVATNDGLMMVE"
     gene            complement(3851..4867)
                     /locus_tag="PLI16_03830"
     CDS             complement(3851..4867)
                     /locus_tag="PLI16_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83718.1"
                     /translation="MKKLPDILIFTIIISVFFYSCTCNRNQEEKNLTELKDTTVQISI
                     KRYEKSLFNLKKEDLQNELKKIKPDYSFFLEGDLDDQNNIEQIRSYLNDPQMIENFTA
                     SQEKFANVTNLEKQLSSSLTYFKYYFPDKKIPGVYTYICGMDYEHPVIYADSVLLIAL
                     DMYLGSGYPLYQALALPLFIRKTMSEEYIVRDCMMAMAEYYCYQEMKDATCLDQMIYE
                     GKKHYFIDAMMPEINDTIKFKYSKKQLDWCYQNEAQMWAFFIDNKLLYSKDDLSYKKF
                     FNEAPFTTSFSKNSPPRTGSWIGWRIVSAYMKKTKATNLNELIRNNNSQEILTQSGYK
                     PKRP"
     gene            4984..5766
                     /locus_tag="PLI16_03835"
     CDS             4984..5766
                     /locus_tag="PLI16_03835"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH83719.1"
                     /translation="MKQSVKYLILSTFLVFILSAAAQAQDRKFNFGIHIAPNVSWMKP
                     DVDGGEFYKYEKNGGRFLMGYGADFNYFFIPNIGIGTGVNVNYNGGKLKYPTVYYARD
                     PNTGIVIDTIQSNLSRKYKLQYLEIPLVLIGSTGDLMGKFSIYGKFGIGTAFKLRARA
                     DDEYVVRNNVEKSYTQKNVNIGDSTLFIRESLIIGVGGTYKFEKIISINFGINFNSGF
                     TNVFSDSYNEEVELAYRNNPNIKRVKESAKANYIEMYVGILF"
     gene            5792..>7023
                     /gene="nadE"
                     /locus_tag="PLI16_03840"
     CDS             5792..>7023
                     /gene="nadE"
                     /locus_tag="PLI16_03840"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOH83720.1"
                     /translation="MKIALAQLNYHIGNFELNTKKIIETIGKAKAEGASLVVFSELAV
                     CGYPPRDFLQYDYFISSCEQAIRQIAAACEGIAAIVGAPSKNPHPAGKNLFNTAFFLA
                     GGQVKFMQHKTLLPNYDIFDEYRYFEPNRSFGIVEYENQRIALTICEDLWNISPDPLY
                     VANPMEKLAGLNPDIVINIAASPFSYTQHEKRKEVLENNVTRYGIPIVYVNHVGAQTE
                     LIFDGGSMAMDRRGKIALQMPFFEEKVSYFDTEQAGQPEETAGSFTRTQLIFDALVMG
                     IKDYFGKMGFSKAILGLSGGIDSALTLVLAAEALGKENVFAVLLPSQFSSEHSVADAI
                     ALAENLGCRYEIIPIAPAFHTVSELLKPVFEDRPFNVTEENIQARLRAVILMAISNKF
                     GYILLNTSNKSEAAVGYGT"
BASE COUNT         1828 a         1458 c         1604 g         2133 t
ORIGIN      
        1 gcggtgcgaa aaagttattg ccgtcatttt gtgttcctcc gagaaaggtt gaggcataaa
       61 gttgtgtgcc gtcggactta agtttggaaa catacatatc aacaccaagg ttgaaagtca
      121 gcacatagtc gtaagtaata tctgtccctc cgttaaaggt ggggtcaaaa gcgctgccgg
      181 caatgggaaa gttggctgag cccgtggtgc caaacaccag caggttgttg tttatgtcca
      241 ctatcaggct gtgtggcagg tcattcctgt ctcctccgag gtaggtggca aataacctgt
      301 tttggcctgt ggcatcatat tttatcaggg caatatcgca gccgccgtta taggtgcctt
      361 cgccaccagc aaagttgacc tgaaaagctc ccggtgatac cgggtagcca ttggttatgt
      421 cattaaaaca aattccgcct gagtaagtgt tgccaaattc gtcgtaggtg gcggtaaaac
      481 cccagttgtc ggccaaagaa cccgtatagg tcgaaaaaat cagtaccgga tcgataatca
      541 ggtcgtagtt tttattgtat ccttcgggaa atttaaaact gacacggttt ctttgtaagg
      601 caaatttgca gggtacactg actttttctc cgtcaatgat ctgaaatgcc acaggggcta
      661 gttcggtaac ctggtttacc gtggttttga tgatcaggtt gctttgttgt atcgaaatat
      721 cggtggctcc ttggtattca ataagtattt tgtttggatt ggctccctgg tgcactgtga
      781 actgatactt taaaagaaat ttttcttcga aaaattccat gtcgatattg tcgtataagt
      841 tcttataagt caacattttg tagttgcgta cgcgggaagc ccattttgat ttatcgttgc
      901 ccaaatagta atttgtataa tcgctgccgc aacctgccgg gatgatttcg gtgtaagggt
      961 ttgcattgat aaagctggca gcataagcgt ggtaattaat aataaattca tcaggattca
     1021 cgaccagatg ctgatttttc gctcttaatt tataactgag cagccagtcg agttttgcct
     1081 gatcgcgaaa tacaaactga attttattct gcaaacattg gagatatcct ccatcaaggt
     1141 ctgctttgta tttaatttgc gggtcccatt gccctttatt ttcaacaaac ctcaggtctc
     1201 ccgaactaat ggaatccttt ggatgggaca tcgcggccca acttatcagc acaaaaactg
     1261 ttaaaagaga aaacttccgc atgcacaatg caatttgaaa acaaatataa tcaaaagtct
     1321 ggagatattt tgttcgtcgt aagtttttta cgtggtcacc cggttttttt gtatggtttt
     1381 tcagtttgtg ttacccctgc acctcgaatt atggcggatt tattcgttgt tcataaaacc
     1441 ttgtttgtgt atcacgtcta ataaactttt ggagaaatgg atattgtcat tttttgtgta
     1501 acgtttccgg gtaaatatct gtgccagggt ttcaagttga ttttcgatga gaagactttt
     1561 tgtcaatggc agttcttctt tttctttgta aagcttgtcg attttttcgg gggtatatgt
     1621 ttcatactgt tcattgtgga cgatggcctc caacggcagg cgatccgtaa gtccgggttc
     1681 ttcgtcggga taaccaagaa ccaatgtggt aaccggtact accccgtcag ggcacccaaa
     1741 aaactcaatg agcttgtcgg cattgtaggt ggtggtgccc aggtagcaaa tacccaatcc
     1801 gttggcttct gcggcaaggg ccacattttg ggcggcgagc agggcatcaa tggctgctgt
     1861 aaaaaacgaa aggaaattgt cgtagcccgg ttcggcattg cgctggcggc accattggtt
     1921 gaaacggtga taatcggcac agaaggttag cagcacaggc gcttccagca ccattttctg
     1981 gttgaaatga tattcgcaaa gtttcttttt tttctcttcg tcgcgtgtaa caatgatgct
     2041 gtatacctgc atgtttcctg tggtagaagc ccgtgttcca gcttgcagaa tcccggttaa
     2101 caagtcttcg ggaatgttgt ttcttttgta ttttctaatg gtccggtggt tgaaaatagt
     2161 gtcgatggtg tccataggtt tatttttctt ttgattttat tctgttgatg attttttcat
     2221 tctggagttc tttactggca tcggtaccta tccaccgggc ggttttgttt tccgaaagca
     2281 tcagtttttg ggccacggcc agggcttccg ggtacagcgc ggtgtttctt tttccaattt
     2341 gtcgaagcgc ccagttgacg gccttgcgga caaagttgcg gtcatcggtg gattctctta
     2401 ctatcagcgg aagaaattca ataaattgcc ggtcatccat tttttttgca tgaacagcca
     2461 ggcaggctat catagtaaaa ccggcccttt taacgaattc ttcatgcctg gtagtccatt
     2521 ctgttgattt attgatggca aacgctgttt tatcaaataa attactgcag cattggtcgc
     2581 aaacatccca tgaatcaaaa tcatgcgccc attgttccat ttgtgcttct gttacctgtt
     2641 tgcagtcatc tatcatggtg gccatgatcc gtgcttcatg atgcccgctt tgccacaggt
     2701 ccagggcgag ggcgcgattt tttttgtacg atttggcttt ttgccggagc gccggcaacg
     2761 gcaccccgaa agcttgtcct gtggcgatgc caaagcgcgc catacctttc acattggaag
     2821 gattgctgtg ttcttgcaaa aagtgcagga tatcctgagt ttctttcatg gctaccggtt
     2881 tattccacca tcatcaggcc gtcgttggtg gcaacccatt tacggttttt ggagtcaata
     2941 gctactttat tcacaaagaa actcagcacg catgaattta acggcgtata taatttccat
     3001 cccgtttcgc cccaccggat aaggcccgtg ttggtagcca gccagaagac attgtttttg
     3061 tcgcccgtaa ccttatagtc gcaaaactgc ggtagtcctg aattttcgtg gttatagact
     3121 gtccaaccgg tatcgtttat tttaattatt ccgctccaat aaaccaccca cacaccaccc
     3181 gactgatcgg tgtaaatgga attgacctga ttgttgggca attgcgaatt agccgtatta
     3241 aatacctgcc agtcacggcc gtcgaatttt gcaaagccgt tggcagtggc aacccatttg
     3301 ttgttatggg catcaacgca caccgcattt accgtattgg agggcagtcc ggaattttcg
     3361 gcggtgaaga cattccagtt tttcccatca tgactggcca gcccgtgttc gctggcaacc
     3421 cacaaaacat cttgccggtc ttcagccagg tcggtaatat gagggttggg caggggacag
     3481 ttggcgggag tataattaat ccattccctg tcgtcatatt tgtaaagtcc gtgaaagcgg
     3541 ctgcctaccc acttatcgcc gttccggctg attaaaatag acgtaacaaa cctgtcgttt
     3601 aacccggaat tgctcgaatc aaacacagtg tagcgggcac catcatattt caccagcaat
     3661 cccccataaa agcagtcgcc cgtagcaaac cagatgttgt cgtcgttatc aatggcaacg
     3721 gtttttatca cgatatccgg aatatcagag ttgagcgtat cgtatgattt gcaggtgaaa
     3781 tctgcaggct gtgaaaataa aaatccggtt gagattacca gaatgaaaaa aaatgccgtt
     3841 gggtatttca ttagggtctt ttgggtttat atcctgactg agtaaggatt tcctgtgaat
     3901 tgttgttccg gatcagttca ttgagattgg tggcttttgt ctttttcata taagcgctga
     3961 caatacgcca tccaatccag cttccggtgc gcggcggcga gtttttcgaa aacgaagtgg
     4021 tgaaaggtgc ttcattaaaa aatttcttgt aggaaaggtc atccttactg taaagaagtt
     4081 tgttgtcgat gaaaaatgcc cacatctgtg cttcgttctg ataacaccag tcgagttgtt
     4141 ttttcgaata tttgaatttt atggtgtcgt tgatttcggg catcatggca tcgatgaagt
     4201 aatgtttttt tccttcataa atcatctgat caagacaagt ggcatctttc atttcctggt
     4261 agcagtaata ttcggccata gccatcatgc aatctctgac gatatattct tccgacatgg
     4321 tttttcggat aaacagcgga agggccaggg cctggtacag cggatacccg ctgcccaggt
     4381 acatgtccag ggcaatcaaa agaacgctgt cggcataaat caccgggtgt tcgtagtcca
     4441 taccgcagat ataagtatat accccgggga tttttttatc aggaaaataa tacttgaaat
     4501 aggttaacga ggacgaaagt tgtttttcaa gattggtaac gttcgcaaat ttttcctgac
     4561 tggccgtaaa gttttcaatc atctgcgggt cgttcagata gctgcggatc tgctctatat
     4621 tattctgatc atccagatcg ccctccagaa aaaaactgta atccggtttt atttttttta
     4681 attcattttg caaatcttct tttttcaggt taaaaagact tttttcgtat cgtttgatgc
     4741 taatttgcac cgtagtgtct ttcaactcag ttaaattttt ttcttcctgg ttccggttgc
     4801 aggtgcagga ataaaaaaat acagatataa tgattgtaaa tatcagaata tcaggaagtt
     4861 ttttcataca ggaaagtttt tttgaagtca aaattaactt gttttaggga agttttttga
     4921 caaaaatatc attttttagc agtaattcat tcataaatgt agtatttttg tattaaaaaa
     4981 aatatgaaac agtcagtaaa atacctcatt ttaagcactt tcctggtttt tatactaagt
     5041 gctgctgccc aggcgcagga tcgtaaattt aattttggca ttcatatcgc gcccaatgtt
     5101 agctggatga aacctgatgt ggatggcggg gagttttata aatatgaaaa aaacggcgga
     5161 cgctttctga tgggctatgg cgctgatttc aattactttt ttatcccgaa tatcggaata
     5221 ggtaccggtg tcaatgtaaa ttacaacggc ggcaaactga aatatcctac ggtgtactat
     5281 gcacgcgacc cgaataccgg aatcgtcatc gatactattc agagtaatct gtcccgcaag
     5341 tataaactgc aatacctcga aataccctta gtattgattg gcagtacggg cgatctgatg
     5401 ggaaaattct ccatctatgg caagtttggc atagggacag cttttaaact cagagcacgt
     5461 gctgatgatg agtatgtggt caggaataat gtggaaaaat cgtacaccca gaaaaatgtc
     5521 aatataggcg acagcacctt gtttatcagg gaatcgctga taataggcgt ggggggtacc
     5581 tacaagtttg aaaaaattat ttctataaat ttcggaatca actttaacag cggttttacc
     5641 aatgttttca gcgacagcta taacgaagag gtggaacttg cttaccgcaa caatcccaac
     5701 atcaagcggg taaaagaaag cgctaaagcc aattatattg aaatgtacgt gggaatttta
     5761 ttctgatacc gcctttagat aattccttac tgtgaaaata gctttggcac aactcaatta
     5821 ccatatcggt aattttgagt tgaatacgaa aaaaattatt gagactatcg gaaaagcgaa
     5881 agccgaaggc gccagtttgg tggtgttttc tgagctggct gtctgcggtt atccgccccg
     5941 tgattttctg caatacgatt attttatctc ttcctgtgag caggccataa ggcagatagc
     6001 agcggcatgc gaaggtatag ccgccatcgt gggcgctcct tcgaaaaacc ctcatccggc
     6061 cggtaaaaat ttattcaata cggctttttt tctggccggt ggacaggtga aatttatgca
     6121 gcataaaacc ttgctgccca attatgatat ctttgacgag taccgctatt ttgagccaaa
     6181 ccgcagtttc ggtattgtcg aatacgaaaa ccaacgcata gcccttacca tctgcgaaga
     6241 tctttggaat atcagtcccg acccgcttta tgtagcaaac cccatggaaa agcttgccgg
     6301 tcttaatccg gatattgtca ttaacatcgc cgcttcaccc ttctcctata cacagcatga
     6361 aaaacgcaaa gaagtgttgg aaaataacgt tacacgatat ggaattccga ttgtatatgt
     6421 caatcatgtg ggtgcgcaaa ccgaactgat ttttgacggc ggatcgatgg cgatggacag
     6481 gaggggaaaa attgcattgc agatgccttt ttttgaggaa aaagtatctt attttgacac
     6541 ggaacaggca ggccagcccg aagaaaccgc tggttcattt acgcgcacac agttgatttt
     6601 cgatgcgctg gttatgggca taaaagatta tttcgggaaa atgggatttt ccaaagctat
     6661 actgggtttg tcgggcggca ttgattcggc actcaccctg gtgctggctg ccgaagccct
     6721 gggcaaagaa aatgtgtttg ccgtattgct cccttcgcaa ttttcgtcgg aacattcggt
     6781 tgccgatgcg atagccctgg ctgaaaacct gggatgccgg tacgagatta tcccgatagc
     6841 ccccgctttt catacggtat cagaactgct aaagccggtt tttgaagacc gtccttttaa
     6901 tgttaccgaa gaaaatatcc aggcccgcct gcgtgcggtg atattaatgg ctatttccaa
     6961 taaatttggt tacattttac tcaatacatc caataaaagt gaggcggccg tgggatatgg
     7021 aac
//
LOCUS       DAOSFP010000087         6971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_9432_length_6971_cov_4.098034, whole genome shotgun sequence.
ACCESSION   DAOSFP010000087 DAOSFP010000000
VERSION     DAOSFP010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9432_length_6971_cov_4.098034"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..638)
                     /locus_tag="PLI16_03845"
     CDS             complement(66..638)
                     /locus_tag="PLI16_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83721.1"
                     /translation="MKKILFLLFMLTWYLPLTGQNFSYRAGKCNTKSDFCPKIALFHQ
                     TSYNFGSLGVNSVNVDFIVFCNRNYMASIRAGINYLSFPRAHAAGAPLEFNFMIGRGA
                     WLFEAGLGVDYLYFYKEYPKFEGIVNANFSYLAATGMIGVRYESINGLFLRVGYNPHI
                     TVLGFDKAEPLIKHPFVYMAAVGIGYTFDN"
     gene            807..3119
                     /locus_tag="PLI16_03850"
     CDS             807..3119
                     /locus_tag="PLI16_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83722.1"
                     /translation="MIVTRFRYFLLFVLLSLVFESVVAQKASIKGFVYDKETGEPAIF
                     TSVYLHKTTIGATTDINGFFAITQIPPGDYILMATFIGYDTVKMPVTLGKGENLNKKL
                     YLQKLAIEMEVVNIQGEAVDKKTETNVSKDVVNIEKIERIPAVGGTPDLAQYLQVLPG
                     VVFTGDQGGQLYIRGGSPVQNKVLLDGMIVYNPFHSIGLFSVFDTDILKNADVYTGGF
                     GAEFGGRISSVMDITTRDGNKKRMSGKVDVNTFGAKLLLEGPIVKQKEDDKANASFIF
                     SAKNSYLKQTSKVLYQYVDTVGLPFNYTDLYGKISVNAPNGSKVNFYGYNFNDRADYR
                     QVATFKWNSFGAGANFVIIPARASMLIDGAFAYSSYNISLTDQTELPKSSRINGFNLG
                     LNFTSFMGKNELRYGIEVLGFGTNFNFYNSALRLIEQKESTTEFALFVKYKWILGKFL
                     IEPSFRAQEYASLSTFSPEPRLAVKFNATKYLRFKLAGGMYAQNLISSTSDLDVVNLF
                     YGFLSGPDNLQDEFDGKEVTHALQKASHVILGVEIDILKNLTLNIEGYYKWFTQLTNI
                     NRSKVYNDSPEYSSKPDVLKKDFIIEKGDAEGVDFTLKYEYRSLYLWAVYSYGFIHRY
                     DGYDTYYPHYDRRHNVNLVGSYNFGKSLTWGVNVRWNLGSGFPFSQTQGFYELLDFSG
                     GVNTDYTTDNGTMGIIYAKYDSGRLPYYHRLDVSFNKVFHFGKNMMLDINLGVTNVYN
                     RKNIFYFDRITYKRIDQLPIMPSLGISFKF"
     gene            3164..4291
                     /gene="hemW"
                     /locus_tag="PLI16_03855"
     CDS             3164..4291
                     /gene="hemW"
                     /locus_tag="PLI16_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOH83723.1"
                     /translation="MAGIYIHIPFCKRKCHYCNFYSVPSLRYKDELIAALHNELLLQK
                     NYLDETVETIYFGGGTPSLLAVDDVKRIIDTVRDNYLLHSNPEITLEANPDDVDAQKV
                     MELKNSEINRVSIGVQSFFHEDLKYLNRIHTSEQAEYAIKALQDAGLENLSIDFIYGM
                     PSLGQEHWRKNLSRAVDMQVPHISAYAITVEPNTTLETLILSQKKQPVSEAEGASEYR
                     YVMKYLPENNYIHYEISNFCLPGFQSKHNSMYWENKPYLGIGPSAHSFNLKTRSWNIN
                     NIEAYLAAINNKTVPFESEELTVNQQFNEFVLMGLRTNRGVSVKAVGDLFGPEKSGYF
                     QKILKKYENKDLLAVNGDNYQLSDEGKLFADRIISDFFVVD"
     gene            complement(4263..5474)
                     /locus_tag="PLI16_03860"
     CDS             complement(4263..5474)
                     /locus_tag="PLI16_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83724.1"
                     /translation="MKTLKRIFRVLILLLLIILIGFGIFLFYFTQKDYQPVPVEETMI
                     YNRKPVQLIDSVPLTFISWNIGYCGLSKEMDFFYDGGSMVRPDKNMLQKNLNGVFDFL
                     SKNDSVDFLLIQEIDSSSRRSYYTNQVSIFGESVKSHCRSFATNYHVGFVPLPVLKPM
                     GKVLSGLATFSKYTPEICERYAYPVNYSWPMKIFMLDRCFMMQRHKLSFSDKELVIIN
                     LHNSAFADADLLRQYETWMLRGFLLSEYEKGNYVIAGGDWNQNPSAYDQLKFISGYAK
                     KTGMPKVMKDLFPASWKFVYDTVLPTNRDNNTPYRHGNTPTTIYDFFVVSPNITVEQI
                     NVIDSRFEFSDHQPVYMRVLLNCDPINICADTVKRYVTGLQDSIQKLNERYCPGSLKA
                     KPLSPPQKNQK"
     gene            5638..6183
                     /locus_tag="PLI16_03865"
     CDS             5638..6183
                     /locus_tag="PLI16_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83725.1"
                     /translation="MKIFEKIKLTFLILLVVCLVGCVIFRTGTFCWISIISFIALSIV
                     SLLQAALFFNIEYKFWLLKYFLAITLSIHSFCFVALMLSYQQIGDWKNILIVTNVFFW
                     INFLFLLIFFKHFLEKEIKKIFLINILIPALVIFFLGVIPLVVPESELYDNFNRERNH
                     QTYDEYIERNDNHNPDQILTF"
     gene            6275..>6971
                     /locus_tag="PLI16_03870"
     CDS             6275..>6971
                     /locus_tag="PLI16_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83726.1"
                     /translation="MDKKKILLFAVIGLFIILVVFFIAKSRSQTLVKINPAFKEYIAA
                     FTSGVISTESTIKVRLNLDLADSSMFEQPVEEALFDFSPGISGKTFWIDTRTIEFRPE
                     KKLTPGKLYTASFFLHKIINVPDSMKTFVFQFHTAKQDFEVSVDNHKAYDYNVPGWEK
                     VTGSLVLADVAEPDDVQSLLEASQGGRRLNVKVFPGSTKKNFSFTIDSVNRGVSASVV
                     TINWNGKAIDSKTK"
BASE COUNT         2029 a         1458 c         1456 g         2028 t
ORIGIN      
        1 ctaatttcgg ggtccattat attctaatgc tacgcgttag aaaatcttta atgcgtaatt
       61 tggatttaat tgtcgaaagt atatcctatt ccaactgccg ccatatagac aaaggggtgt
      121 tttatcagcg gttctgcctt gtcgaagccc agcactgtaa tatgagggtt gtatcccacc
      181 cttaaaaaca aaccgttgat gctttcataa cgcactccaa tcatcccggt ggcggccaga
      241 taggaaaaat tggcatttac tattccttcg aatttggggt actctttgta aaaataaaga
      301 tagtcaacgc caagccctgc ctcaaaaagc caggccccgc ggccaatcat aaaattgaat
      361 tccagtggcg caccggcggc atgtgcacgg ggaaaagata aataattgat cccggcccgt
      421 atgctggcca tataattacg gttgcaaaaa acaataaaat ccacattgac agagttgact
      481 cccaggctcc caaaattgta ggaagtttgg tgaaacaagg ctattttggg gcaaaaatca
      541 ctcttggtat tgcatttacc cgcgcggtat gagaagttct ggcccgttaa gggcaggtac
      601 caggtcaaca tgaacagcaa aaacaggatt tttttcatgg tgtctttttt ttgtgccgtt
      661 ttttttgaaa aatttgttcg taagcaaaaa ctgtttgaaa ataatgtttt ttaagtaata
      721 caaacgcaaa ataactgttc tttattcaga tatttccaaa aaatatctct aattttgaca
      781 aaattatagg gtttgatatt tattttatga tagttacaag attccgctat tttttactct
      841 ttgttttatt atcgctggtc tttgagtcgg ttgtagcaca aaaagcttcc atcaagggtt
      901 ttgtttatga taaagaaacc ggcgaaccgg ccatttttac cagcgtatat ctgcacaaaa
      961 ccaccatcgg ggccactacc gatatcaatg gatttttcgc tataacacaa attccgccgg
     1021 gcgactatat tctgatggct accttcatag gttacgatac cgtgaaaatg cccgttactt
     1081 tgggcaaagg cgaaaacctg aacaagaaac tctatcttca gaaactcgct atcgagatgg
     1141 aagtggtaaa catacagggc gaagccgtgg acaagaaaac cgaaaccaat gtttccaaag
     1201 atgtggttaa tatcgaaaaa atagaacgca taccggccgt tggcggtacc cccgacctgg
     1261 cgcaatacct tcaggtgttg cccggtgtgg tgtttaccgg tgaccagggc ggacaactat
     1321 atatccgggg cggttcaccg gtccaaaaca aggtgctcct ggacggtatg atagtgtata
     1381 accccttcca ttctattggt ctcttctctg tttttgacac ggatatcctg aaaaatgccg
     1441 atgtttatac cggtggattt ggcgctgagt ttggcggacg gatctcctcg gtgatggata
     1501 ttaccacccg agacggcaat aaaaaacgca tgagcggaaa agtggacgtc aacactttcg
     1561 gggcaaaact acttctcgaa gggcctatcg ttaaacaaaa agaagacgac aaggccaatg
     1621 cctcctttat tttctctgct aaaaattctt acctcaaaca aacatcaaaa gtgttatacc
     1681 aatatgtgga caccgtggga ctgccgttta actataccga cctgtacgga aaaatttctg
     1741 tgaacgcacc caatggcagt aaggtgaatt tttacggata caatttcaac gatcgggccg
     1801 attacaggca ggtggccacc tttaaatgga attctttcgg agccggagcc aactttgtta
     1861 ttatccccgc acgggcctcc atgcttattg atggcgcttt tgcctattct tcctataata
     1921 tttcactcac cgaccaaacc gaactgccca aatcctcccg catcaacggg tttaacctgg
     1981 gactcaactt tacttctttc atgggtaaga acgaactgcg ctatggcata gaagtgcttg
     2041 gttttggaac gaattttaat ttttataact cagctctccg gctgatagaa caaaaagaat
     2101 cgaccaccga atttgccctg tttgtaaaat ataaatggat acttggtaaa ttcctcatag
     2161 aaccaagctt cagggctcag gaatacgcct cgctgtccac cttctcgcct gaaccccgcc
     2221 tggccgtgaa atttaatgct accaaatacc tgcggttcaa actggcggga ggtatgtatg
     2281 cgcaaaacct tatcagctct acctccgacc tggatgtggt taacctgttc tacggcttcc
     2341 tttcgggccc tgacaacctg caggacgagt ttgacggcaa agaggttacc catgccttgc
     2401 aaaaggccag tcacgtgata ctgggcgtgg aaatcgacat cctgaaaaat ctgacattaa
     2461 atatcgaagg ttattacaaa tggtttacgc aattgaccaa tatcaaccgc agcaaagtgt
     2521 ataatgattc tcccgaatat tcgtccaaac ccgatgtgct gaaaaaagat tttattatcg
     2581 agaagggtga tgccgaaggt gtggacttta ccttaaaata tgagtaccgt tcgctatacc
     2641 tctgggcggt gtattcttat ggttttatcc atcgttacga cggctatgat acatattacc
     2701 cgcattacga ccgcaggcat aatgtgaatt tagtggggtc ttataatttt ggaaaatcac
     2761 tcacatgggg tgtcaatgtg cggtggaacc tgggatcggg attcccattt tcgcaaacac
     2821 agggatttta tgaattactg gatttttcag gcggtgtcaa caccgattat acaaccgaca
     2881 acggaaccat gggtattatc tatgccaaat acgacagcgg aaggcttccc tattatcaca
     2941 gactcgacgt gtcttttaac aaagtgttcc atttcggaaa aaatatgatg ctcgatataa
     3001 accttggtgt aaccaatgtt tacaaccgga aaaatatttt ctattttgac aggataacct
     3061 acaaacgtat agaccaattg cccattatgc ccagtcttgg catcagtttt aagttttaga
     3121 aacctgtatt agtagcagcg ttttgtaaac aatacttctc cctatggcag gaatatatat
     3181 tcacatacct ttttgtaaaa gaaaatgcca ttactgcaat ttttattctg tcccctcgct
     3241 gagatacaaa gatgaactta tcgccgcctt gcacaacgaa ctcctgttgc agaaaaacta
     3301 cctggacgag acggtcgaaa ccatttattt tggaggagga acaccttcgc tgcttgctgt
     3361 tgacgatgta aaacgcatca tcgatacggt cagggataat tacctgctgc atagtaatcc
     3421 tgaaatcact ttggaagcca accccgatga tgtggatgca caaaaggtca tggagctgaa
     3481 aaactccgaa attaaccgtg tgagcatcgg tgtgcaatcg ttttttcatg aggatttgaa
     3541 atacctcaac cgcatacata cttccgaaca agccgagtat gcaataaaag ccctgcagga
     3601 tgcaggcctg gaaaatttaa gtatagattt tatttatggc atgccctccc tgggccagga
     3661 acactggcgc aaaaatctca gcagagccgt agatatgcag gtaccgcaca tttctgcata
     3721 cgccatcacc gtggagccca ataccacact ggaaactctc atccttagcc agaaaaagca
     3781 accggtttct gaagccgaag gagccagtga gtaccgctat gtgatgaaat acctgccgga
     3841 aaataattat atacattacg aaatatcgaa tttctgtctg cccggctttc aatcaaaaca
     3901 caattcgatg tattgggaaa acaagccgta tttgggcatt ggcccttcgg cacactcttt
     3961 caatctgaaa acccggagct ggaacataaa taatattgag gcctatttag ccgccatcaa
     4021 caacaaaact gttccttttg aatcggagga gttaactgtc aatcagcagt ttaatgaatt
     4081 tgtgctgatg ggactgcgca ccaacagggg cgtgtccgtc aaagcagtgg gagacttgtt
     4141 cggaccggaa aagtccggct attttcaaaa gatattgaaa aaatacgaga acaaagacct
     4201 gctggcggta aacggtgata attatcaact ttctgatgag ggaaagctat ttgctgaccg
     4261 tattatttct gatttttttg tggtggactg aggggctttg cctttaggga acccgggcag
     4321 tacctttcat ttagtttctg tatggaatcc tgcaggcctg tcacatatct tttgaccgtg
     4381 tcggcacaga tatttattgg gtcgcaatta agcaaaaccc tcatatatac aggctgatga
     4441 tcggaaaact caaagcggct gtcgataaca tttatctgtt caacagtgat attgggcgat
     4501 accacaaaaa aatcgtaaat ggtagtcggg gtgttcccgt gccggtaagg cgtattgttg
     4561 tcgcggttgg tgggcagcac cgtgtcatat acaaatttcc aggaggcagg aaaaagatct
     4621 ttcatcactt tgggcatacc ggtttttttg gcataaccag atataaactt tagctgatca
     4681 tacgcggatg ggttttggtt ccagtcgccc ccggcaatga cataattgcc tttttcgtac
     4741 tcgctgagca aaaatccccg cagcatccag gtttcgtatt gccttagcaa atcagcatcg
     4801 gcaaaggcag agttgtgcag attgataata accagctctt tgtcgctgaa tgacaatttg
     4861 tgcctttgca tcataaaaca acggtcgagc ataaagattt tcataggcca ggaatagttt
     4921 acggggtatg cgtaccgttc acaaatttcc ggggtgtatt tcgaaaatgt cgccaggcct
     4981 gataaaacct ttcccatagg tttcaacacg ggcagcggca caaaccctac atggtagttg
     5041 gtagcaaaac tgcggcaatg gctttttacc gattcgccaa aaatggatac ctggttggtg
     5101 taatagctgc gtctcgagga ggagtctatc tcctgtatca acaaaaaatc cacactgtcg
     5161 tttttgctca gaaagtcaaa aaccccgttc aggtttttct gaagcatgtt tttgtcgggg
     5221 cgaaccatac tgccgccgtc gtaaaagaaa tccatttctt tgctgaggcc gcaataacca
     5281 atgttccagc ttatgaatgt cagcggcaca ctgtcaataa gttgtaccgg tttgcggttg
     5341 tagatcatcg tttcttcaac tggtactggc tggtagtctt tttgggtaaa ataaaataga
     5401 aaaattccga aacctattaa gattatgagg agtaaaagta tcagcacacg aaaaattctt
     5461 tttagtgttt tcatcgacaa aaatttttat taaaagtaca aaaaagtatt aatacgtacc
     5521 actgagtgaa aataataatt gtgtgttttt tattgttaat aatcttgcaa ttttataaaa
     5581 tttaattaat tttgcataaa aaaaacaata aagtgttgtt ttttaagatg gtgatggatg
     5641 aaaatctttg aaaaaataaa gcttactttt ctgatactgt tggttgtatg ccttgtgggg
     5701 tgtgttattt ttcggaccgg gactttttgt tggatcagta tcattagttt tattgccctg
     5761 agcatcgttt ctttgctgca ggcagcatta ttctttaata tcgaatataa attctggctg
     5821 ttaaaatact ttcttgccat taccctctct atccatagtt tttgttttgt tgccctgatg
     5881 ctcagctatc agcagatcgg tgattggaaa aatatcctta tcgttaccaa tgtgtttttc
     5941 tggattaatt ttctttttct tcttattttt ttcaaacatt ttttggagaa agaaataaaa
     6001 aaaatatttc tgataaatat acttatcccg gctctggtca ttttttttct tggggtcatt
     6061 cccctggttg ttcccgaatc cgaattgtat gataatttca acagggaacg caatcatcag
     6121 acttacgatg aatacattga aagaaacgac aatcataatc ccgatcagat acttactttc
     6181 tgataactac agctaaaaaa ataatttttg ttgttttaaa gaaaacggta aatatttttt
     6241 aattttaccc ataacaactc taataattta acttatggat aagaaaaaaa ttttactgtt
     6301 tgcggtaata ggccttttca taattcttgt agtgtttttt attgcgaaat ccagatcaca
     6361 gacactggta aaaattaatc cggcgtttaa agaatatatt gccgctttta catcgggagt
     6421 tatctcaact gaatccacta taaaggtacg cctgaatctc gacctggcgg attcctccat
     6481 gtttgaacaa ccggtcgaag aagcgttgtt tgatttttcg cccggcatta gcggaaaaac
     6541 tttctggata gatacccgta caatagagtt tcggcccgaa aaaaaactga cccccggcaa
     6601 actatatacg gccagttttt ttcttcataa aataattaat gtgcccgaca gcatgaaaac
     6661 ttttgttttc cagtttcata ctgccaaaca ggactttgag gtcagtgttg ataatcataa
     6721 ggcttacgat tataatgttc cgggatggga aaaggttacc ggctcgctgg tattggccga
     6781 tgttgccgaa ccggacgatg tgcaatcctt gcttgaggca agtcagggcg gacgaaggct
     6841 caatgttaaa gtttttccgg gcagcaccaa aaaaaacttc tcgttcacta tcgacagcgt
     6901 taaccggggc gtatcagctt cggttgttac catcaattgg aacggcaaag ccattgactc
     6961 taaaaccaaa g
//
LOCUS       DAOSFP010000088         6864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_9631_length_6864_cov_3.942135, whole genome shotgun sequence.
ACCESSION   DAOSFP010000088 DAOSFP010000000
VERSION     DAOSFP010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6864
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9631_length_6864_cov_3.942135"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLI16_03875"
     CDS             <1..383
                     /locus_tag="PLI16_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83727.1"
                     /translation="ALPEAVERVAAILEKYIENDIHVIKVGLHPSDEYAPGGKLVAGP
                     FHTSLREMAMTKVWKRNLIQHILPEQKNSSRQDITITVEDSQYNYAIGYKSENKNYLK
                     QYFNRVRFRRSSEMQKIQFDVDYC"
     gene            367..1059
                     /locus_tag="PLI16_03880"
     CDS             367..1059
                     /locus_tag="PLI16_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83728.1"
                     /translation="MWIIVDKRMPVQAKNKLKTFGDLIEFESSGIVYEAISGHPDIFM
                     CPSGNELILAPNIPEIYPQIFSGNSIPFAFGTTPLGNKYPRTAHYNAAVSDKYLIHNI
                     KHTDEALLQKCKNKTVINTRQAYTRCNLLVLDDTSFITSDSFTGNLLHKLKLNVCYVS
                     PENIVLPGFPNGFFGGCCGIHQKKLYISGSLTYLNAKNNVIDFVEKCGYDIIELYNGP
                     LVDTGGLFFINS"
     gene            1120..3087
                     /locus_tag="PLI16_03885"
     CDS             1120..3087
                     /locus_tag="PLI16_03885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83729.1"
                     /translation="MKLSVIIVNYNVKYFLEQCLHSVIKAGQGIDMEVFVVDNNSVDG
                     SVAMVREKFPEVKIIENSDNKGFSKANNQAIRQSCGEYVLLLNPDTVVEEDTFSRVVG
                     FMDAHPEGGGLGVKMLDGKGHFLPESKRGLPTPAVAFYKVFGLARLFPKSKIFGKYHL
                     GYLDKDKIHEVDILSGAFMLLRKKALDNAGLLDEEFFMYGEDIDLSYRITQAGYKNYY
                     FPETRIIHYKGESTKKSSVNYVLVFYNAMIIFARKHFSKKNASYLSFLIKAAIFFRAF
                     LAIAHRFLKHVFTPLLDATIIFMGIYFIKNYWESHVIFREGGTYPIEFIAIAVPVYIL
                     TWLLSVYFSGGYDKPVRPLKIIQGVALGTVLILTVYALLGEQQRFSRALILLGAGWAA
                     ASMVSLRFLFHLLHFKQFKLGSEDNKRFIIIGEEEEAQRVAALLQKTLISYGFIGLVN
                     TNEKYDATKGFIGNISQLADIIYIYKIDEVIFCSKSMPPQLIIDKMTELQHTNTDYKI
                     APPESMTIIGSNSINTGGELYVLNVNAISKVSSRRSKRLLDIISGLLLLGLFPLAAFV
                     VERPLGFFKNIFFVLAGAKSWVGFCKSETGELRKLPAIRKGVLNPTDTLKLQHISNEI
                     ADSLNILYAKDYRISTDISIMLKGFRSLGRK"
     gene            3201..4940
                     /locus_tag="PLI16_03890"
     CDS             3201..4940
                     /locus_tag="PLI16_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83730.1"
                     /translation="MLLKKGFSCCFGLFFFLLLSANNAFTQKNVEASGDEKIARQFFL
                     FGDYATALKEYQFLYARDSNNPDYLYPLGICYLNTNIDKLKSIFFLEEVTRQKEYDPE
                     AMYQLGVAYMVAYRFEEAIECFQQFKKLISNNKDLNYIPLERQIEMCENAMELIKNPV
                     NVTFENAGARINSAYPDYNPYVNKKETVIYFSSKRQGNIGNLLDYDGYYTSDIFYAEN
                     KYGQWEKSKRMVTTINTPLVEECCGLSADGSYLFAFLDNLETKMQAAMAVKEGKSFQR
                     FTVLGPNINTSKSSATSVTISNDKKTLFFASAREESIGGSDIYMAKLLPSGQWGAPKN
                     LGDEVNTPYDEDYPYLAPDGKTLYFASIGHNSMGGYDIFKTTWNKEDNTFSTPVNIGY
                     PINTPEDNTTISFTGSGRYAYVSALRKDTYGNLDIYRVIFNDVKAAYTTLVGSLVESD
                     SLSIFENFYQTMKSTADSLKEVTDSAYIIQNGISDSLAKVYKQNLTIAENKLEKGPAV
                     SIVVHDNISKKLIGTYKPNKRTGRFIIILPPGEFDIKINCEDYNELTETIKIADREIP
                     AAQITQAFRLTKK"
     gene            4945..5775
                     /locus_tag="PLI16_03895"
     CDS             4945..5775
                     /locus_tag="PLI16_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH83731.1"
                     /translation="MKLKLSKPLAIFDIETTGLNIATDRIVEICIIREDVNGETLVKT
                     MRLNPTIPIPPMVTAIHGITDEDVKDCPTFNDIAHDLNQFLDNCDMAGYNSNKYDIPL
                     LVEEFLRAGIEFNLANRRFVDVQNIFHRMEPRNLKGAYKFYCQKDIQNAHSAEADTRA
                     TYEILMAQLDRYLDVEFTDNDGKVSKPIINDVQALHNFSFKSQNVDLVGHIVYNENNV
                     EVFNFGKHKGKPVEEVFQKEPSYYDWMSKAEFPLSTKKVINAIKLRGFNKSSVKISPK
                     "
     gene            5772..6383
                     /locus_tag="PLI16_03900"
     CDS             5772..6383
                     /locus_tag="PLI16_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOH83732.1"
                     /translation="MKIICIGRNYSKHIKELNNRPPDEPVFFMKPDTALVVRNRPFFY
                     PDFSTDIQHEVEIVFRICKVGKYIQEKFARTYYNEIAVGLDFTARDLQAEAIKNGLPW
                     EKTKSFDGSAPISSFVPLDNFTDIKNISFSLKKNDMVVQQGNTSEMIFSIDKIIAHVS
                     RFITLKMGDLIFTGTPAGVGPVKIGDKLTAYIEDKKMLTCNIR"
     gene            complement(6440..6787)
                     /locus_tag="PLI16_03905"
     CDS             complement(6440..6787)
                     /locus_tag="PLI16_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83733.1"
                     /translation="MKSNRIIVILISLGIFFVSCEGPKTFSVTVIDKATRQPVDSVLV
                     VVKVKAGNKEKSAYNLEGLTDSSGKFMRDEMIGYGLSLKRWHFYMEYDKKGYVHKTET
                     DRTEGVVELEPAQ"
BASE COUNT         2058 a         1549 c         1412 g         1845 t
ORIGIN      
        1 tagcgctccc tgaagccgtg gaacgcgtgg cggcaattct tgaaaagtac atagaaaacg
       61 atatccatgt gataaaagtt ggcctgcacc caagcgatga atatgctccc ggcggaaaat
      121 tagtggcagg acctttccat acttcgttgc gcgaaatggc tatgacaaaa gtgtggaaaa
      181 ggaatcttat tcaacacatt ttaccggaac aaaaaaacag tagccggcag gatatcacaa
      241 tcacagtgga agattctcaa tataactatg caattggtta taagtctgaa aataaaaatt
      301 atctaaagca atactttaat cgggtgcgtt tccggcgttc aagtgaaatg cagaaaattc
      361 aatttgatgt ggattattgt tgacaaacgc atgcctgtcc aggcaaaaaa caaactgaaa
      421 actttcggag acctgattga gtttgagagt tccggcatag tttacgaagc catcagcgga
      481 caccccgaca ttttcatgtg cccttccggc aatgaattga ttcttgcacc caacattccc
      541 gaaatctatc ctcaaatttt ttccggaaat agcatccctt ttgcttttgg cactacacct
      601 ttgggcaaca aatatccccg cacggcacat tataatgcag ccgttagtga taaatatctg
      661 attcacaaca taaagcacac tgatgaagcc cttttgcaaa aatgcaaaaa taaaaccgta
      721 ataaatacgc ggcaggcata tacccgatgc aacctgctgg tcctggatga caccagtttt
      781 attacctccg acagcttcac cggcaacctc ttgcacaaac tgaagctgaa tgtttgttac
      841 gtcagtcccg aaaacattgt gctcccggga tttcctaacg gtttctttgg aggctgctgc
      901 ggcatccatc aaaaaaaatt gtacatcagc gggtcgctca catatttaaa tgcaaaaaac
      961 aacgttattg actttgttga aaaatgtggt tatgatatta ttgagctgta caatggccct
     1021 ttggttgata ccggaggact gttttttata aattcataaa acattttgtc aatatttgag
     1081 taaaaaagta cttttgaagc ctgattagta attttcgaga tgaaactgtc ggtaataatt
     1141 gtaaactaca acgtaaaata cttcctggaa caatgccttc attcggttat taaagccgga
     1201 caaggtattg atatggaggt ttttgtggtg gacaacaatt ccgttgatgg ctcggtggcc
     1261 atggtgcgtg aaaagtttcc ggaagtgaaa atcattgaaa acagcgataa taaaggtttt
     1321 tcaaaagcca acaaccaggc catcaggcaa agttgtggcg agtatgtgct gttgttaaat
     1381 ccggacaccg tggtggaaga agatactttt tcgcgggttg tcggctttat ggatgctcat
     1441 ccagagggcg gcgggctggg agtgaagatg ctcgacggca aaggacattt tctgcccgaa
     1501 tcaaaaagag ggctgcccac gccggcagtt gcattttata aagttttcgg gctggccaga
     1561 ctctttccca aatcaaaaat tttcgggaaa taccatctgg gataccttga caaagacaaa
     1621 attcacgaag tggatatatt atcgggcgct ttcatgctgt tgcggaagaa agcccttgat
     1681 aatgccggat tgcttgacga agagtttttt atgtatggcg aagatatcga cttgtcttac
     1741 cgcattacac aagccggcta taagaattat tacttccccg aaacccgcat cattcattac
     1801 aaaggcgaga gtaccaagaa aagcagcgta aattatgtac ttgtttttta taatgcgatg
     1861 attatttttg cccgaaagca cttttcaaag aaaaatgcca gctacctgtc atttctgatc
     1921 aaggcagcca tttttttcag ggcttttctg gctattgccc atcgtttttt aaaacatgtt
     1981 tttacaccgc tgctcgatgc cacgataatt ttcatgggta tttatttcat taaaaattat
     2041 tgggagtccc atgtgatttt tcgcgaaggc ggaacgtatc ctatcgagtt catcgccatt
     2101 gccgtgccgg tttatatttt aacctggctg ctctctgtgt atttcagcgg gggttacgat
     2161 aagcccgttc gtccgctgaa gataatccag ggtgttgcct taggcaccgt gttgatactc
     2221 accgtatatg ccttgctggg tgagcagcag cgtttttcaa gggcgctgat cctgttgggc
     2281 gcaggatggg ccgccgcatc gatggttagc cttcgctttt tgtttcacct gctgcatttt
     2341 aaacagttta agctgggctc ggaagacaat aagcgcttta tcattatagg tgaagaggaa
     2401 gaagcacaac gagtagctgc cttgttgcaa aagaccctta tcagctatgg ctttatcggt
     2461 ttggtaaata ccaacgaaaa atacgatgcc accaagggct ttatcggcaa tatatcccaa
     2521 ctggccgaca tcatttatat ttataaaatt gatgaggtga ttttttgctc caaatccatg
     2581 ccgccacagc ttatcatcga taagatgacc gagttgcagc ataccaatac cgattataaa
     2641 attgctcctc ccgaaagcat gacaataata ggcagcaact ccataaatac cggtggcgaa
     2701 ctttatgtgc tcaatgtcaa tgccatcagc aaagtttcca gccgccggag caaacgactg
     2761 ctcgatatta tttcaggatt gctgctgttg ggtttgtttc cgctggcagc ctttgttgta
     2821 gaacgccccc ttggattttt taaaaatata ttttttgtgc tggcaggagc aaaatcatgg
     2881 gtgggctttt gcaaatcaga aaccggagag ctacgcaaac tgccggctat ccgcaaaggc
     2941 gtcctcaatc ccaccgacac cctcaaactt cagcatatct caaacgaaat cgccgacagt
     3001 ctcaacatcc tgtacgccaa agactacaga atatccacag atatcagtat tatgctgaaa
     3061 ggctttcgca gcctcggacg aaaataagtt cgcaaagacc attggcgtgt gtttataact
     3121 tctgataaaa acattgtcac tgacttcgag tttttatatt tttgcctgaa gattttttta
     3181 aacaaaacca ccatattcag atgctattaa aaaaaggatt ttcatgctgc tttggattgt
     3241 tttttttctt gcttctttca gctaataatg cctttactca aaagaatgtc gaagcctcag
     3301 gcgatgagaa gattgcccgg cagttttttc ttttcggcga ttatgccacc gcgctcaaag
     3361 aataccagtt tctgtacgcc agagattcta acaaccctga ctatctctat ccgttgggta
     3421 tctgttatct gaacaccaat atcgacaagc tgaaatccat cttttttctg gaagaagtta
     3481 cccgtcagaa agaatacgac cccgaggcca tgtaccagct cggcgttgcc tatatggtgg
     3541 cctatcgctt cgaagaagcc atcgaatgtt tccagcaatt caaaaaactt atcagcaaca
     3601 acaaagacct gaactacata cccctcgaac ggcagattga gatgtgtgaa aatgcgatgg
     3661 aactgataaa aaatccggtt aatgtcactt ttgaaaatgc cggagcccgc atcaactcgg
     3721 cctaccctga ttacaaccct tacgtaaaca aaaaagaaac ggtgatttat ttttcatcca
     3781 aacgccaggg caacatcggc aacctgctgg attacgacgg atattacacc tcggatattt
     3841 tttatgccga aaacaaatac gggcaatggg aaaaatcaaa gagaatggta actaccataa
     3901 acactcctct ggtggaagaa tgttgcggcc tgtctgccga cggctcttac ctttttgcat
     3961 ttcttgataa cctggagact aaaatgcagg ccgccatggc tgtgaaggaa ggaaaaagtt
     4021 ttcagcggtt cacggtgctg gggcctaata tcaacacatc caaatcaagc gccacctccg
     4081 taacaatttc taacgacaaa aaaacacttt tctttgcctc agccagggaa gaaagcattg
     4141 gtggcagcga catctatatg gcaaaactgc ttccgtcagg acagtggggc gctccaaaga
     4201 acctcggcga tgaagtcaac acaccctacg atgaagacta tccttatttg gctcccgacg
     4261 gaaaaaccct gtattttgcc tctatcggcc ataacagcat gggaggctat gatattttta
     4321 aaacgacctg gaacaaggaa gacaacacct tttcaacgcc tgtcaatatc ggatatccga
     4381 taaatacgcc tgaagacaat accactattt cctttaccgg atcaggcaga tacgcctatg
     4441 tatcggcatt gagaaaagat acctatggga acctggatat ctaccgtgtc atctttaacg
     4501 atgtaaaagc ggcctatact acccttgtcg gcagcctggt ggagtcagac tccctgagta
     4561 tcttcgagaa cttttatcag accatgaagt caacagccga cagcctgaaa gaagtcaccg
     4621 actctgccta cattatccag aacggcatca gcgattcgct ggccaaagtc tataaacaaa
     4681 acctgacaat cgctgaaaac aaattagaaa aaggcccagc ggtttctatc gttgttcatg
     4741 ataacatctc caaaaaactg atcggtacct acaagcccaa caagcgtacc ggcagattta
     4801 tcattattct tcccccggga gagtttgata taaaaataaa ctgcgaagat tacaacgaac
     4861 tgaccgaaac cataaaaatt gccgacaggg aaattcccgc cgcacagata acacaagcat
     4921 tcaggctcac aaaaaaataa aaccatgaaa ttgaaacttt ccaagccgct ggctattttc
     4981 gatatcgaaa ccaccgggct caacattgct actgatcgta tagtagagat ctgtataata
     5041 cgcgaggatg tgaacggcga aactttagtc aaaactatgc gcctgaatcc tactatacct
     5101 attccgccaa tggttacggc cattcatggc atcaccgacg aagatgtgaa agattgccct
     5161 acatttaacg atattgccca tgatctaaac cagtttctcg acaactgcga tatggctgga
     5221 tataattcca acaaatacga cattcccttg ctggtagaag aattcttgcg tgccggcatc
     5281 gaatttaacc tggccaacag gcgttttgta gatgtgcaaa atatctttca ccgcatggag
     5341 ccccgcaacc tgaaaggagc ctataagttt tactgccaga aagacattca aaatgcacac
     5401 tctgctgagg ccgacaccag ggcaacctat gaaatactga tggcacagct cgacaggtat
     5461 ctcgacgtgg aatttaccga caacgatggc aaggtcagca aacctattat caacgatgtc
     5521 caggcgcttc ataacttctc ttttaaatcg cagaatgttg atttggtagg acatatagtg
     5581 tacaatgaaa acaatgtgga agttttcaat ttcggaaaac acaaaggcaa accggtggaa
     5641 gaagtctttc agaaagaacc gtcgtactac gactggatgt cgaaagccga atttccttta
     5701 tctaccaaaa aggtaatcaa tgccatcaag ttgaggggat tcaacaagtc atcggtaaaa
     5761 atctcgccca aatgaaaatc atctgtattg gccgcaatta cagcaagcac atcaaagagt
     5821 taaacaaccg ccccccggac gaaccggtgt tttttatgaa acccgatact gcgctggtgg
     5881 tgcgcaaccg tccgtttttc tacccggatt tttccaccga tatccagcac gaagtggaaa
     5941 ttgtttttcg aatctgtaaa gtaggcaaat acatacagga aaagttcgcc cgtacctatt
     6001 acaatgaaat tgccgttggt ctcgatttta ccgcccgcga cctgcaggcc gaagccatta
     6061 aaaacggact gccctgggaa aaaaccaaaa gctttgacgg atcagcgcca atcagcagct
     6121 tcgtgcctct ggataacttc actgacatca aaaacatcag tttttctttg aaaaaaaatg
     6181 acatggtcgt tcaacaaggc aatacttctg agatgatttt cagtatcgac aaaatcattg
     6241 cccacgtatc acgctttatt accctgaaaa tgggcgacct gatatttacc gggaccccgg
     6301 cgggtgtagg gcctgtgaaa ataggggata agctgaccgc ctacattgaa gacaagaaaa
     6361 tgctgacttg caatatcagg taagacagtg aagcctgccc cggaattaca atacgttata
     6421 tgaagattta tttgtcattc tattgtgctg gctctaactc caccacacct tccgtgcggt
     6481 cggtttccgt tttatggaca tagccttttt tatcatattc catataaaaa tgccagcgct
     6541 tcaatgaaag gccatagccg atcatttcgt ccctcatgaa tttcccggat gaatcagtaa
     6601 gaccttctaa attataagca cttttctctt tgttgccagc cttgactttt acaacaacaa
     6661 gcacactgtc aacaggctgc ctggttgctt tgtcgataac ggttaccgaa aaagttttcg
     6721 gcccctcaca ggaaacaaaa aatattccta aactaattaa aatgacaatg attctattgc
     6781 ttttcatttt atatgccttt atatgcggtt ttttgatttt ttataagaat tgtttttatc
     6841 agaacaccaa atttatagat taat
//
LOCUS       DAOSFP010000089         6720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_9904_length_6720_cov_4.496324, whole genome shotgun sequence.
ACCESSION   DAOSFP010000089 DAOSFP010000000
VERSION     DAOSFP010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9904_length_6720_cov_4.496324"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="PLI16_03910"
     CDS             <1..868
                     /locus_tag="PLI16_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HOH83734.1"
                     /translation="DWMMNLVEEMVEKIAYDTNGNTQIKVGDNIIDFKRPWKRFTMFE
                     AIKHFTGIDISAMDEPALRQTATKLGVETTEAMGKGKLIDEIFGATCEPKLIQPTFIT
                     DYPVEMSPLAKKHRSKEGLVERFEAIVNGKELCNAFSELNDPIDQRQRFEAQLELGRR
                     GDDESMVLDEDFLRAIEYGMPPTAGLGMGIDRLAMIMTNSHSIQDVLFFPQMKPEIKS
                     VVISAEDFVAKGIPAEYAELIMKAGFPTLEALKTAVPNKLFNSICGLRKKLKLDIKAP
                     TIEEVAAWINNA"
     gene            931..2214
                     /locus_tag="PLI16_03915"
     CDS             931..2214
                     /locus_tag="PLI16_03915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH83735.1"
                     /translation="MHPTKTPYLLYSDDKGNIFEDTSLLVCGRTANKTVELNAEDLIA
                     LPEGSDFFHLPGRRAIGYDPKTNKFRTCEKGWAVAAFAAPAYTLTHHAVWKTEAGAPR
                     LPLYAYAAIGWLDQKFYSTIFRIDPDIRQDCDQFNQFYVEKNAKRMLKKQPANRLLKH
                     LSHCALVYFCPAARNYFMNRWEAPLPTSPTCNSRCVGCISYQPKDHDICSSQNRITFV
                     PTPEEIAEIAVEHLNTAPNPVVSFGQGCEGEPLLVWETIREAIKLIRSKTQKGIINLN
                     TNASKPEAVEELCKAGLQSIRVSMNSAQEKNYQKYYNPTNYSFADVMKSIQIARKYNR
                     WVSVNYFVFPGYTDEKNEIQALKNLIRNYDISMIQWRNFNIDPEWYNEIVLPEKDSNC
                     YGIKNLIEEIRKDFPKIYHGYFNPGEEIISTRLNP"
     gene            complement(2247..2669)
                     /locus_tag="PLI16_03920"
     CDS             complement(2247..2669)
                     /locus_tag="PLI16_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83736.1"
                     /translation="MKHLLKSVLMLACVFSLMLPGCKKYEDGDTFSLLCKMYVVNKWK
                     IEKQFMYNSGADITPANNDWLHLKSDFRYVQYQSDTVASEGNWALDGKKEEFIMIPDD
                     YVLGTQIGMVYTIVRLENKRFRIRSNSTNPIETHYIPR"
     gene            2844..3368
                     /locus_tag="PLI16_03925"
     CDS             2844..3368
                     /locus_tag="PLI16_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOH83737.1"
                     /translation="MNPKEERFRKIVEEQQQRIRSICRYYSRSDEDTQDLQQEVLINI
                     WRSLDNFRGDSAISTWIYRIAVNTSLNYIMKENRRSQFNLTLNDQHFNILMQDDEHEH
                     KIETEKKLDGLHEIINQLNVIDKLLITLSIEKLTTKEISDIIGITEPNVRTKLHRIRE
                     ELKQKIQGGNYEKQ"
     gene            3355..3954
                     /locus_tag="PLI16_03930"
     CDS             3355..3954
                     /locus_tag="PLI16_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83738.1"
                     /translation="MKNNNNIAPELHEETQHAANFSNTENLIARMRKEDNRNKKIMFG
                     MFILYLVFTIFYAILFIFNPDPKLSLTDRMAGHSYVLAFIIGTFYFIWEYLIYKKVDY
                     STPLLTLLKKTVRRYRFFSIKWIPVMIVVVIISYGFTISYVERLATWDTGICTKILTI
                     QAVYWGIMVIAGLIGYLIWRKRSRLIWKDAKTLLEELKN"
     gene            4711..>6720
                     /locus_tag="PLI16_03935"
     CDS             4711..>6720
                     /locus_tag="PLI16_03935"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOH83739.1"
                     /translation="MNSEKKLYLLDAMALIYQAFYAYRDRPLINSKGVNTSAVYGFAN
                     ILYKLLKEEKPTHIGVAFDTMAPTLRHDEYIHYKANRQETPEDLVSSIPYIKRLIEGF
                     NIPALYVDGYEADDVIGTLAKEAEQAGYTVYMVTPDKDFGQLVSDNVFIYKPARFGDK
                     ATIMGPREVCERFGIQKPEQVIDMLGIWGDASDNIPGIYGIGEVGARKLVNEFGSLEK
                     ILENTDSITPEGLKQKVIAGRDLAIQSKALATIILNVPIAFEENKLIMEHPNENILKE
                     LFEELEMRTFAKRVFTDISLQKQGAAQAQLNSQPDLFSEVPQENEPQTEGSLKNIYNT
                     PHEYILVDTAEKRNALIAELANCKEFCFDTETTGIDPNAAEMVGLSFCMQPHKAYFIR
                     LPNEYHDACQLVQEFKHLFENQEKLKIGQNLKFDMEMLKWYDVEVKGPLFDTMLAHYL
                     IEPDMRHNMDYLSQSYLNYEPVPIEKLIGKKGKNQLSMAIISDEEIKEYAAEDADVTF
                     QLKQIFEPLLKEANVTSLFKDVEIPLIPVLASMETEGVRLDKTALNDYSAQLQTEIQQ
                     LEKEIFELAGLEFNISSPKQLGDVIYGHLKIIENPKKTATKQFSTAEDVLIKLVNKHP
                     IIQKLLDYRSLTKLKSTYVDSLPELINTRTGRIHTSYNQAVAATGR"
BASE COUNT         2188 a         1488 c         1405 g         1639 t
ORIGIN      
        1 cgattggatg atgaacctgg tggaagaaat ggtggagaaa attgcttacg acaccaacgg
       61 aaatacgcag ataaaggtag gcgacaatat tattgatttc aaacgtccat ggaaacgttt
      121 tacgatgttt gaagctataa aacactttac aggcatagat atttctgcca tggacgagcc
      181 ggcactgagg caaacagcaa ccaagcttgg cgtagaaacc actgaagcca tgggaaaggg
      241 caaactgatt gacgaaatat tcggcgccac ctgcgaaccc aaattgatac aaccgacatt
      301 tatcaccgac tatcctgtgg aaatgtctcc cctggcaaaa aaacaccgca gcaaagaagg
      361 cctggtggaa cgttttgaag ccatcgtgaa cggcaaagag ctctgtaatg ccttttcgga
      421 actcaacgac cccattgacc agcggcaacg ttttgaagcg cagctggagc tgggcaggcg
      481 aggcgatgac gagtcgatgg tgctcgacga agattttttg cgggccattg aatacggcat
      541 gccgcctacc gctgggttag gcatgggcat cgaccgcctg gccatgatca tgaccaactc
      601 gcactccata caggatgtat tgttcttccc ccagatgaaa cctgaaataa aatcggtggt
      661 aatttcagct gaagattttg tagccaaagg catccctgcc gaatatgccg aactgataat
      721 gaaagccggg ttcccgacgt tggaagcctt gaaaacagca gtccccaaca aactctttaa
      781 cagcatctgc ggattacgaa aaaaactaaa actcgacatc aaagcaccta ctatagaaga
      841 agtagccgcc tggataaaca atgcgtgaaa accacggagg cacagatcca ccgcgacaga
      901 aaatcctgac tttctaatta acgtaaaaaa ttgcatccta caaaaactcc atacttatta
      961 tatagcgacg acaagggaaa tatctttgaa gatacttcat tgttggtttg tggaagaaca
     1021 gcaaacaaaa ctgtagaatt aaatgccgaa gaccttatcg cattacctga aggcagcgat
     1081 ttttttcact tgcccggcag gcgtgctata ggttacgatc caaaaacgaa caaattccgt
     1141 acttgtgaaa aaggctgggc cgtggcggct tttgcagctc cggcatatac gcttactcac
     1201 cacgctgttt ggaaaactga agccggcgca ccccgtttgc cactctacgc atatgccgct
     1261 atcggctggc ttgaccaaaa attttattcc actattttcc gtatcgatcc cgatatcagg
     1321 caggattgcg accagtttaa ccagttttat gttgagaaaa atgctaagcg catgctcaaa
     1381 aaacaacccg ccaatcgctt attgaaacat ctttcgcatt gtgcgctggt gtatttttgt
     1441 cctgccgccc gcaactattt tatgaaccgc tgggaagccc cgctacccac ttcgcccaca
     1501 tgcaattcgc gatgtgttgg ttgtatttcc tatcagccca aagaccacga catatgttct
     1561 tcgcaaaacc gtatcacctt tgttcccacc ccggaagaaa tagccgaaat tgccgttgag
     1621 cacctgaaca ccgccccaaa tccggtggtc agttttgggc agggctgcga aggagagccc
     1681 ctgctggttt gggaaactat tcgcgaagcc atcaaactga taagatcaaa aacacaaaaa
     1741 ggcatcatca acctcaacac caatgccagc aaacccgaag cagttgaaga attatgcaag
     1801 gccggcctgc aaagcatccg tgttagcatg aacagtgcac aggaaaaaaa ctaccagaaa
     1861 tattacaacc ccaccaatta ttcttttgcg gatgtgatga aaagcataca aattgccaga
     1921 aaatacaaca ggtgggtttc ggtcaattat tttgttttcc cgggctacac cgacgaaaaa
     1981 aatgaaattc aagccctgaa aaaccttatc agaaattatg acatcagcat gatacagtgg
     2041 cgcaatttca acatcgatcc cgaatggtac aacgagattg tgctacccga aaaagacagc
     2101 aactgttacg gtataaaaaa tttaattgaa gaaataagaa aagactttcc aaaaatatat
     2161 cacggatatt tcaatcccgg cgaagaaatt atcagcacaa ggctgaaccc ttaattgata
     2221 taaaagacaa tacctgctcc cgataattac ctggggatat aatgggtttc gatgggattg
     2281 gtactgttcg acctgatgcg gaacctttta ttttcgaggc ggacgatggt ataaaccatc
     2341 ccgatttgag tccccagaac ataatcatcg ggtatcatga tgaactcctc tttctttccg
     2401 tccaaggccc aattaccctc agatgcaacc gtatcggact gatactgaac ataacgaaaa
     2461 tcgcttttca aatgtagcca atcgttgttg gccggggtga tatcggctcc gctattgtac
     2521 ataaattgtt tttcaatctt ccatttattt accacataca ttttgcatag cagtgaaaag
     2581 gtatcgccat cctcgtattt cttacatccc ggcagcatca aagaaaacac gcaggcaagc
     2641 atcaacactg atttgagcaa atgtttcata aaaattgatt tttggggtta ctacaaaaat
     2701 aaacattttt caaagaaaaa ttttctgcat catgttgttt ccaaccatga aagctaaaaa
     2761 agttaacttt acagggagaa aaaattacag aaaatgtaac attttttcct taaaatcatc
     2821 aaaaacccaa acactaaatc acgatgaatc ctaaagagga gagatttcga aagatagtgg
     2881 aggaacagca acaacgcatc cggagtattt gccggtatta ttcacggtcg gatgaggaca
     2941 cccaggacct gcagcaggag gtacttatca atatctggcg cagcctcgac aacttccggg
     3001 gcgattcagc catcagcacc tggatatacc gcattgccgt caacacctcc ctgaactata
     3061 tcatgaaaga aaaccgcagg tcgcagttca acctgactct caacgaccaa cactttaata
     3121 tactgatgca ggacgatgag catgaacata agatagaaac agaaaagaaa ttagacgggc
     3181 ttcatgaaat catcaaccag ctcaacgtca tcgacaaact gctgataacc ctatccatag
     3241 aaaaactaac tacaaaagaa atatcggaca tcatcggcat caccgaaccc aatgtacgaa
     3301 ccaaacttca ccgtatccgc gaagaactaa agcaaaaaat tcaaggaggg aattatgaaa
     3361 aacaataaca atatagcacc tgaattgcat gaggaaactc aacatgctgc caacttttcc
     3421 aacaccgaga atcttatcgc caggatgaga aaagaagaca acagaaacaa aaaaattatg
     3481 tttggcatgt ttatcctgta cctggttttt accatctttt acgccatact ttttatcttc
     3541 aatcccgacc cgaaactttc gctcactgac cgtatggcgg gacacagcta tgtgctggct
     3601 tttatcattg gaacctttta ctttatctgg gaatatctga tatacaaaaa ggtagattat
     3661 tctacaccac tgctgacact attgaaaaaa accgtgagac ggtacagatt tttcagcata
     3721 aaatggatac cggtgatgat agtggttgtc attataagct atgggtttac tatttcctat
     3781 gttgaaagac tcgcaacctg ggataccggc atctgtacca aaattttaac catacaggct
     3841 gtttattggg gaataatggt cattgcagga cttataggct acctgatatg gcgcaaacgc
     3901 tcaagactca tctggaaaga tgcaaagaca ctgcttgagg agttgaagaa ttagtaagtt
     3961 agtcgattga gtacagagtt gattaagtta attgagtaca gagttgattg agtaaagagt
     4021 tgatcaagtt aattgattac aaagttgatt aaattgattg agtgcagtgt taattgagtt
     4081 gattaggttg gaaagcttaa gttttgcgta aataagctaa aaagcagaat cttcgatgcg
     4141 agcataaaat atttcccgca gatagcgcag atagccgcag atgaaaaatt aataaatctg
     4201 cgtaaatcag cgggatcagc gggagaaaat agagaagttt ttccgcaaat ggcacagata
     4261 accgcagatg aaaaattaat aaatcagcgc aaatcagcat gatcagcggg agaaaaatga
     4321 gaagttttcc cgtagatagc gcagatagcc gcagatgaaa aaattaataa atcagcgtaa
     4381 atcagcgaga tcagcgggag aaaatagaga agttttcccg cagatggcgc agataaccac
     4441 agatgaaaaa attaataaat cagcgtaaat cagcgatccg cctaggcgga aacgggagaa
     4501 aaatgaagaa tggttcccgt agatggcaca gattgatgca gaaaaacaaa aaataaatca
     4561 gcgtaatcag caggatctat gggagaaaaa cgcatccttt acagcaattt tatgaatttg
     4621 tcccttattt taataaatca ttttccattc agggcatttc gtgaaatatc ccttcttttt
     4681 cctattttta ccgtaaataa tttataatac atgaactcag aaaaaaaact ctacctcctc
     4741 gatgccatgg cgcttatcta ccaagcgttt tatgcttatc gcgaccggcc gctcatcaat
     4801 tctaaagggg taaatacttc cgctgtttat ggctttgcca acattttata caaactgctg
     4861 aaagaagaaa agcccacgca tatcggcgtt gccttcgaca ccatggcccc taccctgcgc
     4921 cacgatgaat acatacacta caaagccaac cgtcaggaaa ctcctgaaga ccttgtcagt
     4981 tctattcctt atataaaaag actgattgag ggatttaata ttcctgcact ttatgtggat
     5041 ggttatgaag ccgacgacgt gataggaacc cttgccaaag aggccgagca ggccgggtat
     5101 accgtttata tggtcacacc cgataaggat ttcgggcaat tggtcagtga taacgtgttt
     5161 atctataaac ctgcacgctt cggggataag gcgactatca tgggccccag ggaagtatgt
     5221 gaaaggtttg gcatacaaaa gccggaacag gtaattgaca tgctcggtat ctggggcgat
     5281 gcctccgaca atattcccgg gatatacggt attggagaag ttggcgcccg taaactggtt
     5341 aacgaattcg gttctctcga gaaaatcctc gaaaacaccg attctattac tcctgaaggg
     5401 ctgaaacaaa aagttatcgc aggccgcgac cttgctattc agtcaaaagc cctggccacc
     5461 atcatcctga atgttcccat tgccttcgaa gagaacaaac ttatcatgga gcatcccaac
     5521 gagaatatat taaaagagct ctttgaggaa ctggaaatgc ggacttttgc aaaacgggta
     5581 ttcaccgaca tttccttaca aaagcagggt gcggcacagg cccagctaaa tagtcaaccg
     5641 gatctgttca gcgaagttcc gcaggaaaat gagccgcaaa ccgaaggttc actaaagaat
     5701 atttacaaca caccgcatga atacatttta gtagatactg cggagaaaag aaatgcgctg
     5761 atcgctgaac ttgccaattg caaggagttt tgttttgata cggagacaac cggtattgac
     5821 ccgaatgctg cggaaatggt tggcttatca ttttgcatgc agccccacaa agcatatttt
     5881 atcaggctcc caaatgaata ccacgacgcc tgtcagttgg ttcaggaatt caaacacctt
     5941 tttgaaaacc aggaaaaatt gaaaataggt caaaacctga agttcgacat ggagatgctg
     6001 aaatggtatg atgttgaggt gaaaggtccg cttttcgata ccatgctggc acattacctt
     6061 attgaacccg acatgaggca caacatggat tatctgtcgc aaagctattt gaattacgag
     6121 ccggttccta tcgagaaact cattgggaaa aaaggcaaga atcaactctc tatggccatt
     6181 atcagcgatg aggaaataaa agaatatgct gctgaagacg ccgatgttac ttttcagtta
     6241 aaacaaatct ttgagccact actgaaagaa gccaatgtaa cttcactatt taaagacgtt
     6301 gaaattcccc tgattcctgt gctcgcctcc atggaaaccg aaggcgtccg gcttgataaa
     6361 acagcgctta atgattattc tgcacagttg cagacagaaa tacaacaact ggaaaaagaa
     6421 atttttgaac tggctggcct tgaatttaat atttcttcac ccaaacaact gggtgacgtt
     6481 atttacggac acctgaagat cattgaaaac cccaaaaaaa cagccaccaa acagttttcc
     6541 acggccgaag acgtgctgat aaaactggta aacaaacatc cgatcatcca gaaattgctg
     6601 gactatcgtt cgctcacgaa gctaaaatcc acctatgtgg atagccttcc agagctcatc
     6661 aatacacgta ccgggcgtat ccacacttcg tataaccagg cggtggctgc cacgggacgg
//
LOCUS       DAOSFP010000090         6698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_9947_length_6698_cov_4.250188, whole genome shotgun sequence.
ACCESSION   DAOSFP010000090 DAOSFP010000000
VERSION     DAOSFP010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9947_length_6698_cov_4.250188"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="PLI16_03940"
     CDS             complement(<1..1476)
                     /locus_tag="PLI16_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83740.1"
                     /translation="MNNAYSALIKKLDLFIRKYYKNQILKGILLFLSFSLALFLILTI
                     LENFAYFNSTVRTVLFYTYIVVNVFVLIQFIIIPLFRLFKIGKVISYEQAAKIIGEHF
                     TEVKDKLLNTLQLKQISDTDTTSLELINAGIEQKISTLSPVQFNAAVDFKVNKKLLKF
                     LLPPAIILLVILIASPAVLTEPTKRYYNYSTFYEKQAPFQFVILNKSLEAVQQQDFVL
                     NVKLTGDDIPGELFVVYNENEYSLNKENVVVFNYTFKNLQKDLTFYLKADEYRSRSYT
                     LKVIPKPIILDFEVQLNYPAYTGKKNEVVENSGDLSVPAGTNICWKLLTKDTKKILLK
                     FVDKSLLLEPVKNEVFLCSRNFLTSQNYTVIPQNQFIKNNDSLTYSINVIPDAYPMVD
                     AKEYRDSVYYSHLYFKGFIKDDYGFNKLLFKYRILKQDDDDTGAELSQNITINKQINQ
                     GEFYHYFDVSALQVSPGDQIEYYFEIWDNDGVNGSKAARSSK"
     gene            1694..5059
                     /locus_tag="PLI16_03945"
     CDS             1694..5059
                     /locus_tag="PLI16_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOH83741.1"
                     /translation="MRKFTLLIVILLFSGLQMAFAQQKTITGKVTSKDDGTTLPGVTV
                     VVKGTTIGTVTDVNGKYTILVPPKYDVLIFSFVGMKSQEIKVTGSATIDVILESDVMN
                     IEGVVVTALGIAREKKSLGYSTQEIKGDLVQTVKSDNFINSLSGKVAGVQVKKNTNIG
                     GSTNVLLRGNKSLTGDNQVLFVVDGVPVNNSNFNTSSQIQGGMGYDFGNMASDINADD
                     IESINVLKGAAATALYGSRAAGGVIMITTKKGTTAGSGVGTEIKKKGIGITVNSGVTM
                     GFVDKSTFIKYQKDYGAGYGHYYEGDPNDEFWYFRDVNNDGVDEQWVVTSEDASYGAP
                     FDASRYVYQWNAVDPESPWYMTATPWIAAENGPITFFQKPLTFNNSVAIDNAGPKGSY
                     RLAYTNFTQKGLLPNSKLNKDNVLLNGSWNVTDKLTVSGQGNYTNTRAVGRNSTGYSD
                     NIMTSFRQWYQTNVDIQQQRELYELTGRNITWNYADPTVAAPIFWDNPYWVRYENYEN
                     DNRNRFTGYMAVDYKVLKWLSLFGRFSADTYTEMQEERKAVGSIPGPFGLGSGSDGSL
                     GRSDQGSGYLRRDINFTEYNFDLMANINKDFGKNWNLRGVVGYNARRTNFNRIISATN
                     GGLAVPGLYSLQNSVGPLPLSKELASKVGVNGIYASASIGYKSLIYLDGTIRRDHSST
                     LPVGNSVYYYPSVAASFVFSNLIPENKVLSYGKVRLNYAMVGNSASYDQLTDNYNIIT
                     PLNSPITSVPGTKKNSNLLPEKTRSIEGGLEMYFFGRRIGFDAAYYNSNTSNQILPLA
                     VSTATGYTYKIINAGQIQNQGVEISLNATPVKTKKFQWNMTINWAKNWNKVLSLAEGV
                     ENLQLGSYQGGVSINARVGQPYGIICGTDYITINGNPVGESDSARLINPANGRFYKTA
                     TSDNVIGNINPDWTGGWLNSFSWGGWNLSFLVDVSMGGDIFSLDMYYGLATGLYDETS
                     YLNDLGNPVRDPITWVDPNDHSLGYASNSGGFINEGVNPDGSENTTRIAANNYGAFGY
                     VRNPDRAFVYDASFVKLREVSLSYSLPAKWLQKCFLTGVTLSAVASNPWIIYKNLPYA
                     DPESGLSAGNLQGYSVGSLPSTRDFSFNVKLTF"
     gene            5071..6504
                     /locus_tag="PLI16_03950"
     CDS             5071..6504
                     /locus_tag="PLI16_03950"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOH83742.1"
                     /translation="MKKILALLLVILMFSGCKKLEDLNENTKDPTDVTGESLFTGAQK
                     NVFDQMVSTNVNLNIFRMFAQYWTETTYTDEANYDLVTRTIPDNHWDILYRDVLKDLD
                     ESARIIQETTYGGDASPSIKKNKLAIIEIMKVYTYSVLVETFGNIPYTEALDIEIPAP
                     KYDDGLTVYKDLIIRLNAAIAELDPAWGSFEGADNMYGGNVNQWLKFANSLKLRMAMV
                     ISDVDPGFAQTQAVAAAQNVIASNADNGSLIYLSAQPNCNPIYDDLVASGRHDFVAAN
                     TLVDAMVALNDPRLPFYFTMAPDTNTYIGGVYGASNDYTQYSHVADMIQLPTFEGIIM
                     DYAEVEFLLAEAVEKGFAVGGTAEDHYNNGITASITYWGGTSADAATYLANPAVAYAT
                     AAGTWQQKIGTQAWIGLYNRGYEAWTSWRKLDFPVLVAPPDAVSDIPVRFTYPIAEQT
                     LNGTNYGAASSAIGGDDVATKLFWDLH"
BASE COUNT         1988 a         1405 c         1431 g         1874 t
ORIGIN      
        1 ttttgatgac cgggcggcct tgctgccatt tacgccgtcg ttgtcccaga tttcaaagta
       61 atactctatt tgatcccctg gtgatacctg aagtgccgac acatcaaaat aatgataaaa
      121 ttccccctga tttatttgtt tgttgatggt gatattttgc gatagttcag cacccgtatc
      181 atcgtcatcc tgttttagta tcctgtactt gaaaagaagt ttgttaaacc cgtaatcgtc
      241 tttaataaat cctttaaaat acaggtggct gtaatacacg gaatcgcggt attctttggc
      301 gtcaaccatg ggataggcat cgggaataac attaatggaa taggtcagcg agtcgttgtt
      361 ttttatgaat tgattttggg gtattacagt gtagttctgg gaagtgagaa agttgcggct
      421 gcacaaaaac acttcgtttt taaccggttc gagcaacaag gatttatcca caaatttcaa
      481 caggattttt ttagtgtctt tggttaacaa tttccagcaa atattggttc cggccggtac
      541 gctgaggtcg ccggaatttt caactacttc attctttttt cctgtatagg caggataatt
      601 caactgcacc tcaaaatcga gaattatggg cttgggtatt actttcagtg tatagctgcg
      661 cgatctgtat tcatcggctt tcagataaaa agtaaggtct ttctgtaaat ttttaaatgt
      721 ataattaaac actaccacat tttctttatt gaggctatac tcattttcgt tgtacaccac
      781 aaataattcg ccggggatat cgtcaccggt gagttttacg tttaagacaa aatcctgctg
      841 ctgcactgct tcaaggcttt tgtttagtat tacaaactga aaaggcgcct gtttttcgta
      901 aaaagtggaa tagttgtaat accttttggt gggttcggtt agcacggcgg gcgaagcaat
      961 aagaatcacc agcaaaataa tggctggcgg caataaaaat ttcaataatt ttttgtttac
     1021 cttaaaatca acagccgcat tgaattgaac cggagacaag gtacttattt tttgttcaat
     1081 tccggcatta atcagctcca gactggtcgt gtcggtatcc gaaatttgct ttagctgcaa
     1141 ggtgttcagc agtttgtcct tcacttcggt aaaatgttct ccgatgattt ttgccgcctg
     1201 ttcatatgaa atcacctttc cgattttaaa gagcctgaaa aggggaataa taataaattg
     1261 tatcaaaaca aaaacattga ccactatgta agtataaaac aaaacggttc ttacggtgga
     1321 attgaaatag gcgaaatttt caagaatggt aagtattaaa aacaaggcta aagaaaaaga
     1381 caaaaacagc aaaatccctt tcagaatttg gtttttgtaa tactttctga taaagagatc
     1441 cagttttttt atgagtgcag agtaggcgtt attcataatg tttacatccc ggcaaaatat
     1501 aacggtttta tgaaagacat attttatttt atgcaaattt acaaaaattt tatggcttta
     1561 gatttctctc gtaatctctt taattcttgt aatttaatat ttattaataa aaatttttaa
     1621 taataaattt ggtttttatg attacatata gtaatattga actcgaattt aactttaacc
     1681 taaaacaaat cttatgagaa aatttacatt gttaattgta attctgttat tttcaggatt
     1741 gcagatggcc tttgcccaac aaaagaccat cacaggtaag gtaaccagta aagatgatgg
     1801 cactactttg cccggtgtta cggtcgtggt taagggcacg accataggaa cggttaccga
     1861 cgtaaatgga aaatacacta ttttggttcc gccgaaatat gatgtgttaa tattttcctt
     1921 tgtggggatg aaatcgcagg aaattaaagt aaccggaagc gcaaccattg atgtgatttt
     1981 ggaatccgac gtaatgaaca tcgaaggtgt agttgttacc gcacttggca ttgccagaga
     2041 aaagaaatct cttggctatt cgacgcagga aataaagggc gacctggtgc aaaccgttaa
     2101 atcagacaac ttcatcaatt ctctttcggg aaaagttgcc ggtgttcagg taaaaaagaa
     2161 taccaatatc ggcggctcca ccaacgtttt gctgcgtggc aacaaatcgc tgaccggcga
     2221 caatcaggtg ctttttgtgg ttgacggcgt tcctgtcaac aacagcaact tcaacacctc
     2281 ctcacagata cagggtggta tgggatacga ctttggcaat atggcttcgg acatcaacgc
     2341 cgatgacatt gagtcaatca acgtactgaa aggtgctgct gctaccgccc tttacggctc
     2401 acgtgctgcg ggcggtgtta tcatgattac caccaaaaaa ggtactactg ccggcagcgg
     2461 tgttggtact gaaataaaaa agaaaggtat cgggattacg gtaaattccg gggtaaccat
     2521 gggctttgtt gacaaaagca cattcattaa gtaccagaaa gactacggcg caggctacgg
     2581 acactattat gaaggcgatc ctaacgatga gttttggtat ttccgtgatg ttaacaatga
     2641 tggcgtggat gagcaatggg tagttacctc ggaagatgct tcctatggtg ctccttttga
     2701 tgccagccga tatgtgtatc agtggaatgc cgttgacccc gaatctccct ggtatatgac
     2761 agccactccc tggattgctg ccgaaaacgg tcctattacc tttttccaaa aaccgctgac
     2821 gttcaacaat tcagttgcta ttgataatgc agggcctaaa ggctcctacc gtctggccta
     2881 taccaatttt acgcaaaaag gtcttttgcc taacagtaaa ttaaacaaag ataatgtgtt
     2941 gttgaatggt tcgtggaatg ttacggataa actcactgtg tcggggcaag gcaattacac
     3001 caacacccgg gctgtgggcc gtaactctac cggatacagc gataatatca tgacttcatt
     3061 ccgtcagtgg taccaaacca atgttgacat acagcaacaa cgggaacttt atgaattaac
     3121 cggaagaaat attacctgga actatgccga cccaaccgtg gccgcaccta ttttctggga
     3181 caacccctat tgggtaagat atgaaaatta tgaaaacgac aacagaaacc gttttacggg
     3241 ctatatggct gttgattata aagtgctgaa atggttaagc ctgttcggac gtttttcagc
     3301 cgatacctat acggaaatgc aggaggaaag aaaagctgtg ggcagcatac ccggcccatt
     3361 cggacttggt tccggctccg acggcagcct tggacgcagc gatcagggtt caggctatct
     3421 gagaagagac attaatttca ccgaatacaa cttcgacctg atggccaata tcaataaaga
     3481 tttcggtaag aactggaacc tgagaggcgt agttggatat aacgccagaa gaaccaattt
     3541 caacagaatt atttctgcca caaacggcgg acttgctgta cctggtttgt attcactgca
     3601 aaacagtgtc ggcccactgc cgctttcaaa agaactcgct tccaaagtag gtgtaaatgg
     3661 catctatgcc agtgcatcaa taggctacaa gagcctgata taccttgacg gaactatacg
     3721 gcgtgaccac tcttccacat tacccgttgg aaacagcgtt tattactatc cttcagtggc
     3781 cgccagcttt gtattctcta acctgattcc ggaaaataaa gtgttatctt atggtaaggt
     3841 acgtttgaat tatgctatgg tgggtaatag cgccagttat gaccaattaa ccgacaatta
     3901 caatattatc accccgctga actccccgat tacttcggtg cccggcacaa agaaaaacag
     3961 caatttactg cccgaaaaaa caagaagtat agaaggcgga cttgaaatgt atttctttgg
     4021 aagacgtata ggttttgatg ctgcttatta caacagcaac acttccaacc agattctgcc
     4081 actggccgtt tccacagcta cgggctacac ctacaaaatt atcaacgccg gacaaattca
     4141 aaaccagggt gtggaaatct ctttaaatgc cacaccggta aaaaccaaaa aattccagtg
     4201 gaacatgacc atcaactggg caaaaaactg gaacaaagtc ctttctttag cagaaggggt
     4261 tgaaaacctg cagttgggtt cataccaggg tggtgtttcc attaatgcca gggtaggaca
     4321 gccttacgga ataatctgcg gaaccgacta tataaccatc aacggtaatc ctgttggcga
     4381 atccgacagc gcccgtctga tcaatcccgc caacggcaga ttttataaaa cagccacttc
     4441 tgacaatgtc atcggaaata tcaaccccga ctggacaggt ggctggctaa actccttctc
     4501 ctggggtgga tggaacctga gtttcttagt tgacgtttct atgggtggag acatcttctc
     4561 gctggatatg tattatggac ttgctaccgg attgtacgac gaaacttctt atctgaacga
     4621 tttaggaaac cctgtaagag atcctattac ctgggttgac cccaatgacc acagcctggg
     4681 ctatgcttcc aatagcggtg gttttattaa tgaaggcgta aatcccgacg gatcagaaaa
     4741 tactaccaga attgccgcca acaactatgg agcatttggt tatgtaagaa atccggatag
     4801 agcctttgta tatgatgctt cctttgttaa actgagagaa gtttccttat cgtacagcct
     4861 gcccgccaaa tggttgcaaa aatgtttcct caccggagtt accttgtcag cagtagcttc
     4921 caacccatgg ataatctaca agaaccttcc ttatgccgac cccgaatcag ggttaagtgc
     4981 cggaaatctt cagggctatt cagtagggtc gctgccatca acccgtgatt ttagtttcaa
     5041 tgttaaatta accttttaaa taatacaacc atgaaaaaga tattagcatt attactcgta
     5101 atcctaatgt tttccggctg caaaaagttg gaagatctga atgaaaacac aaaagatcct
     5161 accgatgtta ccggcgaatc actatttacc ggtgcacaga aaaatgtttt cgaccagatg
     5221 gtgagcacca atgtaaacct caatattttc agaatgtttg ctcaatactg gacggaaacc
     5281 acctatactg atgaggccaa ttatgacctg gttacccgaa ccattccaga caatcattgg
     5341 gatatcctct accgcgatgt actcaaagac ctcgatgaat ctgcccgaat catacaagaa
     5401 acaacctatg ggggagatgc cagtcctagt attaaaaaga acaagttagc cataattgaa
     5461 attatgaaag tttatactta ttctgtactc gtggaaactt ttggcaacat cccttacaca
     5521 gaagctctgg atatagaaat tcctgcgcca aaatacgatg atggccttac tgtttacaaa
     5581 gatttgatta tccgcctgaa tgccgctatc gcagaactgg atcccgcctg gggcagtttt
     5641 gaaggcgcag acaatatgta tggaggcaat gtaaaccaat ggttaaaatt tgccaattcc
     5701 cttaaattaa gaatggctat ggtgatttcg gatgttgacc ctggttttgc acagacacag
     5761 gcagttgctg cagctcagaa tgtaatagct tcaaatgccg ataacggaag cctcatatac
     5821 ttatctgccc agccaaactg taatcctatc tatgatgacc tggtagccag cggaagacat
     5881 gattttgttg ccgccaatac cctggtggac gccatggtag cattaaacga tccgcggttg
     5941 ccattttact tcactatggc acccgacacc aatacgtata ttggcggtgt atatggagct
     6001 tcaaacgatt acactcaata ttcgcatgtg gctgatatga ttcagcttcc tacatttgaa
     6061 ggaatcatta tggattatgc cgaagtagaa tttctcctgg cagaagctgt ggaaaagggg
     6121 tttgctgtgg gtggcaccgc cgaagaccat tataacaacg gaattacggc ctccattact
     6181 tactggggcg ggacaagtgc cgatgccgcc acatacctgg ccaaccccgc tgttgcttat
     6241 gccactgcag ccggcacctg gcagcagaaa atcggaacac aggcctggat aggcttgtac
     6301 aaccgaggct atgaagcgtg gacaagctgg agaaaattag acttccccgt gctggtagct
     6361 ccgcctgatg ccgtatccga tataccggta aggtttacgt atcccatagc cgaacaaacc
     6421 ctgaacggaa ccaattacgg tgccgcctcc agcgccatag gtggtgatga tgtggctacc
     6481 aaactattct gggatttaca ttaagttttt ttagctgtgt aaatcagcac ctttattatc
     6541 aaaggggatt ccggccggaa tccccttttt tattcaaaaa taaacccgta aataaatttg
     6601 gtttataaag aaaacctata actttgtaga aatttttata caaattccta tgacacaaaa
     6661 gaaaaataag gcagtaacaa aagaggatgt tgtggtta
//
LOCUS       DAOSFP010000091         6647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10053_length_6647_cov_3.543234, whole genome shotgun sequence.
ACCESSION   DAOSFP010000091 DAOSFP010000000
VERSION     DAOSFP010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10053_length_6647_cov_3.543234"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..241)
                     /locus_tag="PLI16_03955"
     CDS             complement(14..241)
                     /locus_tag="PLI16_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83743.1"
                     /translation="MPKTYHVIGLMSGTSLDGLDIAYCRFVLSANKWSGEIIKASTNK
                     YPEVWKNKLSQAACIPAPDFMLVHNEYGSYC"
     gene            complement(250..1533)
                     /gene="murF"
                     /locus_tag="PLI16_03960"
     CDS             complement(250..1533)
                     /gene="murF"
                     /locus_tag="PLI16_03960"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOH83744.1"
                     /translation="MNIEQLYNIFLEHPAISTDTRKLTPDSLFFALKGEHFNGNKFAK
                     QALDQGVAYAIIDEKEYVTGDKTILVDNTLECLQKLAAFHRSKLTIPVIGISGSNGKT
                     TSKELIHTVLAEKFNVFSTQGNLNNHIGVPLSVLQITPTHEIAVIEMGANHVGEIQQL
                     CTISQPNYGLITNVGKAHLEGFGNFENVIKAKTELYRYLEQQDGLVFINFNNATLKAH
                     AHNIRQYSYGTNQQANIHTTFIVANPYVKLYWEDKKTIIESKLIGKYNFENILCAIAV
                     GDYFQVSPEQIKHAVEIYFPSNSRSEFIHKEDNTIILDAYNANPTSMIAALENFNLMQ
                     STNKVVMLGDMLELGAYKKMEHEKIIEFLNNSNYNQVFLVGSNFYEANTRNDFLTFKS
                     SQLLRDHLKAHPLHDALILVKGSRGTQMEHVLSAL"
     gene            complement(1602..3074)
                     /locus_tag="PLI16_03965"
     CDS             complement(1602..3074)
                     /locus_tag="PLI16_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH83745.1"
                     /translation="MYIKRLIVFTTVALVATFFLNSCKKEVSNTTAWEYNNPKNGGFE
                     VVPYEEQETGPGLVLIEGGTFMMGRTEQDVLYDWDNYPRRITVSSFYMDQTEVRNLDY
                     CEFLYWTRRVFGVDYPEVLQKRLPDTLVWRDRLAYNEPYVDYYLRHPAYRDYPVVGVN
                     WVQASDYCNWRTDRVNEYILMREGILKMDPNQQNEENFNTDAYLAGQYEGMVKNDLYD
                     MDPNGSGTRKVRMEDGILLPKYRLPTEAEWEFAALGLIGNTIYERVLERRIYPWNGHI
                     ARNDRLNNLGDFVANYKRGRGDNMGVAGNLNDAADITAPVYSYWPNDYGLYCMAGNVS
                     EWVMDVYRPLSFEDYADFRSFRGNVFQTQLRDADGVLEDKDSLGRIQMRDVTVDESKD
                     RRNYRQADNRNYLDGDLTSSMLVGGDWTAEDVETPIMYDYGVNSMINDKARVYKGGSW
                     RDRQYWISPGNRRFLDENLSTDCIGFRCAMTRVGSPVGNY"
     gene            complement(3105..4007)
                     /locus_tag="PLI16_03970"
                     /pseudo
     CDS             complement(3105..4007)
                     /locus_tag="PLI16_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
     gene            4378..>6647
                     /gene="porU"
                     /locus_tag="PLI16_03975"
     CDS             4378..>6647
                     /gene="porU"
                     /locus_tag="PLI16_03975"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOH83746.1"
                     /translation="MMFKKFLLLTCFVFSLSTIYAQQKAITPQNDYKIHWNKPAKLFI
                     TELTKKEFLSFDGAVYADSTDIPYFFIALDVIKSSSVPEVVIKNPVYEELPAADLQLV
                     NAGKIMIGEQINIISRVSYQNKKPLLLVSFVPLRKNSFNGKTERLVSFSLDFSYGKSK
                     ETAGIVKAQQYATHSVLQSGDWYRVDVIRSGIHKITYDDLVSLGMDVSGINPKNLRVY
                     GNGGKMLPENNADFRHDDLAENAIFVYGEDDNVFSQGDYILFYAQGPEYWTLDTAKGV
                     FNHKINKFCNSSGYFITASLGAGKRIGPAQLSAAAPTHTVTTFNNYAFHENDSINLIK
                     SGREFYGEVFDLTTSRTFGFSFPNVVSGSQMYLRTGLLARSVNIGTSFRVYANNSSIG
                     LVIIQAASPVYTSDYARITHAEMNFAAASSLNLRLDYNKGANASAIGWLDYIELNADC
                     YLTFTSGQLAFRDKSSIGQVSEFVLGNASSGITIWDVTDPTGVTRVDATLNASNLVFK
                     MNTDSLKEFVAFDGTSFLPVKVQGKIQNQDLHGLAQYDMIIVAYPDYTGEASRIADMH
                     RNNDNMTVVVTTPQLIYNEFSSGNQDVTAIKGFVKMFYDRAASLSEQPKYLLLFGDAS
                     YDYKNRLENNTNQVPTYESSNSLEPSGSFLTDDYFGFLDNADSGPYNNLLDIAVGRIP
                     VQNIDEAKAVTDKILNYNARYDLISSATSCSGFSDAISNLADWRNVLCFVADDSDLAG
                     EDFLGQSESIANYVDTT"
BASE COUNT         1758 a         1370 c         1510 g         2009 t
ORIGIN      
        1 tttttacctg ttatcaacaa taacttccgt attcattgtg gactaacata aaatcgggcg
       61 ccgggatgca ggctgcctgc gataatttgt ttttccacac ttccgggtat ttattagtgc
      121 tggctttgat gatttccccc gaccatttgt ttgccgaaag cacaaagcgg caataggcaa
      181 tgtcaagccc atccagtgat gtgcccgaca ttaagcctat aacatgataa gtttttggca
      241 tgttcggaat tataaagcgg aaaggacatg ttccatctgg gttccgcggg agccttttac
      301 caaaatcagc gcgtcgtgca atggatgtgc cttcagatgg tctcgtaaca gctgggaact
      361 tttaaaagtc agaaagtcgt tacgggtatt tgcctcataa aagttggagc ccaccagaaa
      421 aacctggttg taattagaat tgttcaaaaa ttcgatgatt ttttcgtgct ccattttttt
      481 gtaggctccc agctcgagca tatcacccaa catcaccact ttgttggtgg attgcatcag
      541 gttgaaattt tccagcgcgg caatcatact ggtggggttg gcattatagg cgtcgagtat
      601 aatggtatta tcttctttgt gaataaattc cgacctggag ttggaaggaa agtagatttc
      661 cacggcatgt tttatctgtt ccgggcttac ctgaaaataa tcgcctaccg caatggcaca
      721 taagatgttt tcaaaattat atttgcctat cagttttgac tctataatgg tttttttatc
      781 ttcccagtaa agttttacat aggggttggc tacaataaaa gttgtgtgga tattggcttg
      841 ctggtttgtg ccgtatgaat actgcctgat gttatgggca tgtgctttga gggtagcgtt
      901 gttgaagttg ataaaaacca ggccgtcttg ttgttcaagg taacggtaaa gttccgtttt
      961 ggctttaatc acattttcaa aatttccgaa cccttcaaga tgagcttttc cgacattggt
     1021 aatcaggccg tagtttggtt gtgaaatagt acaaagctgt tgtatttcgc ctacatgatt
     1081 ggctcccatt tctatcacag cgatttcgtg ggtgggggta atctgcaaaa ccgacagcgg
     1141 aactccgatg tggttattca ggttgccctg ggtggaaaac acattgaatt tttcagcgag
     1201 cacagtatgt ataagctctt tagaggtggt ttttccgttg ctgccgctga taccgataac
     1261 aggaatagtg agtttcgacc tgtgaaatgc cgcgagtttt tgcaggcatt ccagtgtgtt
     1321 gtccaccagg atggttttgt caccggtaac atattctttt tcatctatta tggcataagc
     1381 cacaccctgg tcgagtgcct gtttggcaaa tttgtttccg ttaaagtgtt cgcctttcag
     1441 ggcaaaaaac aatgaatccg gtgtgagttt tctggtgtcg gtagatatgg ccggatgttc
     1501 aaggaagatg ttgtataatt gttctatatt catatttgta tgtaataaaa aaccccgctt
     1561 gcaaaataaa cggggttttt tgaaaataaa aagtattttt tctaatagtt accaacaggg
     1621 ctgccaacac gtgtcatggc gcaacggaat cctatgcagt cggttgaaag gttttcgtcc
     1681 agaaaccttc tgttgcccgg gctgatccag tattgtctgt ctctccatga gccgcccttg
     1741 taaacgcggg ccttatcgtt gatcatagaa tttacgccgt agtcatacat aattggtgtt
     1801 tcaacatctt cagcagtcca gtcgcctcca acaagcatgc tggaagttaa atcgccgtca
     1861 agataatttc tgttatcggc ctggcggtaa tttcttctgt ctttgctttc atccacggta
     1921 acgtcgcgca tctgtatgcg gcctaagcta tctttatctt cgagcacgcc atcggcatct
     1981 ctgagttgtg tctggaaaac atttcctctg aacgacctga aatcagcata atcttcaaat
     2041 gacaacggtc tgtaaacatc cataacccat tccgaaacgt tgccggccat gcaatacaag
     2101 ccatagtcgt tgggccagta tgaataaacg ggcgctgtga tatcagcagc gtcgttgagg
     2161 tttcctgcaa cacccatgtt gtcgccgcgg ccgcgtttgt agttggctac aaaatcgccg
     2221 aggttgttga gcctgtcgtt acgggcaatg tgtccgttcc agggataaat tctgcgttcc
     2281 agtacgcgtt catagatggt gttcccgata agtcccagtg cggcaaattc ccattcggct
     2341 tcggtcggca ggcggtattt cggaagaaga ataccatctt ccatacgcac tttacgggtg
     2401 cctgatccgt tgggatccat atcataaagg tcgtttttta ccatgccttc atattggcct
     2461 gccaggtatg catcggtgtt gaagttttct tcgttctgct ggttggggtc cattttcagg
     2521 ataccttcac gcatcaggat gtattcgttt acacggtcgg ttctccagtt gcaatagtct
     2581 gaggcctgta cccagtttac accaaccacc ggataatcgc ggtatgccgg atgacgcaga
     2641 tagtaatcta cataaggttc attataggcc agacgatcgc gccacaccag ggtgtcgggg
     2701 aggcgtttct gcaggacttc aggataatct acaccaaata cccgtctggt ccagtaaaga
     2761 aattcacaat agtccagatt gcgaacttcg gtttggtcca tataaaaaga ggatacagtg
     2821 attctgcgcg ggtagttgtc ccaatcgtaa agtacatcct gttctgtacg gcccatcata
     2881 aaggtaccgc cttcaatcaa tacaaggcct ggtccggttt cctgttcttc gtaaggaact
     2941 acttcaaaac cgccgttttt cgggttgttg tattcccagg cagtcgtatt ggaaacttct
     3001 tttttacatg agttcaggaa gaatgtggca accagagcca cggtcgtaaa aacgattaat
     3061 cttttaatat acattgttta tcggtttttt gtttctatat ataactaaaa agaggggcaa
     3121 tttattgctc tgactctttt tgagggtggt ttgcaattaa actgaagcgc gaaagacact
     3181 tcgtgggcac cgcctgtagc gtttgtcagg tttgaaatag tgaggtcata gctgtatcca
     3241 accttgaaca tactcacctg taatcctacc ataaatatta tagcgtcaga atttttaaag
     3301 ttttgtctga accacagacc gccaacaaac ggatacctgt taaagtataa tccgtaattg
     3361 atctgctgga agttttgttg ctgcataaaa agaatgttcg gcgacaaggt aggtgtatgg
     3421 tcgttgcttc tgcggcgaac tttttttacc aggttgataa tacctccggc atggacggta
     3481 aattttatgg gcatgcggct tttgctgata aagccttcgt cgggcatggt gagatggtgt
     3541 gcggcaaagc ccacaaaata attttcaccg tatcccagga tgccggctgc aaaatctgcg
     3601 tttcttttgg tcaggcgtga agggggcact tcgttggtgt tgaacacaaa tccgtgcctt
     3661 ttgtcaatca tatcaggaaa agtgagtttc tgccagtcga ggtgtttttg aaaataagct
     3721 gcctgtaagg caagtttcat ggaaaatgac cggtttacct ccagacggaa agaatacata
     3781 aacgaaaccg ttgtggtgtt taatattcct tgtccggcgc ggtcggtatt gagcaggaca
     3841 ccaataccgc ccgataattt ttcgatgtgc tgatcgtagg aggcattgta ggtaacataa
     3901 gtccccgaga tggagggcca ctggtcgcgg aaatttagga tgactctcgg acacaaggcc
     3961 gtgccggcaa aagccggatt aaggtataag gggtttgcat agtattgtga aaaatgcgga
     4021 tcctaagcaa aactgacact tcccatcatt accaacacaa aaacaataca ggtgtaaatc
     4081 tttttcatat cgagtatttt ttagttctga acattataac gaaactttcg gtcattgtat
     4141 tggcaaaaga gtaaaaaatt tacaaaaccc tgcataacaa taaaaatagt cttgttccgt
     4201 tataatgtga tattttaaca agcaaaaata atatattttt aattacacaa aatttccagc
     4261 gacctttttt ttaacatttt tactcatatt taattgtaaa ggttacaaaa tgattatttt
     4321 ttttgataaa atatggtttg gaaaatttta aataatttta cacggttcaa aaaacgtatg
     4381 atgtttaaga aatttttgct acttacctgt tttgtattct cattatcaac aatatacgct
     4441 caacaaaaag cgataacgcc ccaaaacgat tataaaattc attggaacaa gccggcgaaa
     4501 ttatttatta ccgaactcac caaaaaagag tttctgagtt ttgatggtgc ggtatatgcc
     4561 gatagcacgg atattcctta tttttttatc gcactggatg tcatcaaatc atcttctgta
     4621 cccgaggtcg ttatcaaaaa ccccgtatat gaagaactgc ctgctgccga tctgcagctt
     4681 gtgaacgccg gcaaaattat gattggtgag caaattaaca ttatttcccg tgtatcctat
     4741 caaaacaaaa aacctctgtt gctggtaagt tttgttccgt tacgcaaaaa cagtttcaac
     4801 ggaaaaaccg aacgtctggt ctcatttagc cttgattttt cgtatggcaa gtcaaaagaa
     4861 acggccggaa tcgtcaaggc gcagcagtat gccacccatt cggtattgca atcgggcgat
     4921 tggtacaggg tggatgtaat tcgttcaggt attcataaaa ttacatatga tgatttagtt
     4981 tccctgggca tggatgtttc cggtataaat cccaaaaatc ttcgtgtcta tggaaatgga
     5041 ggtaagatgt tgcctgaaaa taatgctgat ttccggcacg atgacctggc cgaaaatgcc
     5101 atttttgtct atggcgaaga tgataatgtt tttagccagg gcgattatat tttattttat
     5161 gcccagggcc cggaatactg gacattggat acggctaagg gggtatttaa ccataaaata
     5221 aataagtttt gcaatagttc cggatatttt ataaccgcca gtttaggtgc cggcaaaagg
     5281 atagggccgg ctcagctgtc ggcagcggcg cctacccata ctgttaccac ctttaataat
     5341 tatgcgtttc atgaaaatga ttccataaat ctgattaagt cgggacggga attttacggg
     5401 gaagtttttg acctgaccac ctcgcggacc tttggcttta gttttccaaa tgttgtcagc
     5461 ggctcacaaa tgtatttgag aaccggtttg ctggcgaggt cagtcaatat tggcacttct
     5521 ttcagggtat atgccaacaa cagcagtata ggtctggtta tcattcaggc ggcttcaccg
     5581 gtatatactt cggactatgc ccgcattacc catgccgaaa tgaattttgc cgccgccagc
     5641 tccctgaacc ttcgtctgga ttataataag ggcgctaatg cttcggccat aggctggtta
     5701 gactatatcg aactgaatgc cgattgttac cttactttca ccagcgggca gttggctttt
     5761 cgcgacaaat catccatcgg ccaggtgtcg gaatttgtgc tgggcaatgc ctcttcgggt
     5821 attaccatct gggatgttac cgaccctaca ggagtgacaa gagtggatgc taccctgaat
     5881 gccagtaacc tggtttttaa aatgaatact gattcactga aggaatttgt ggcttttgat
     5941 ggtacctcat ttcttccggt gaaagtgcag gggaaaattc agaaccagga tttgcacggc
     6001 ctggcgcaat atgatatgat tatagtagcc tatcctgatt atacgggcga agcttcgcgt
     6061 atagccgata tgcaccgcaa caacgataac atgacggtgg tggttaccac tcctcaattg
     6121 atctataatg agttttcttc cggtaatcag gatgttactg cgataaaagg ctttgttaaa
     6181 atgttttacg acagggcggc aagcctcagc gaacaaccta aatacctttt attattcggc
     6241 gatgcttcgt atgattataa aaaccgcctg gagaacaata ccaatcaggt gcccacttat
     6301 gaatcaagca actcactgga accctcagga tcgtttttga cagatgatta ttttggtttt
     6361 ctggataatg ccgacagcgg gccttacaac aacctgctgg atattgccgt gggcaggatc
     6421 cctgttcaaa acatcgacga agcgaaggcc gttaccgata agatattgaa ttataatgca
     6481 aggtatgatt tgatttcgtc cgctacatcc tgttcgggat tttccgatgc tatttccaat
     6541 ttggccgact ggcgcaatgt gctgtgtttt gtagctgatg attctgatct ggccggggag
     6601 gattttttgg gacagtccga aagtattgct aattatgtgg ataccac
//
LOCUS       DAOSFP010000092         6582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10197_length_6582_cov_4.394668, whole genome shotgun sequence.
ACCESSION   DAOSFP010000092 DAOSFP010000000
VERSION     DAOSFP010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10197_length_6582_cov_4.394668"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLI16_03980"
     CDS             <1..430
                     /locus_tag="PLI16_03980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83747.1"
                     /translation="WTNNALNTSVTKNGLSLINGQTYYLSVKTENGAGLKTICSSDGA
                     ILFTPTGISENADESVIFASPNPFNKTTVITYFSASEREITVILTDMYGRELVLSEKT
                     ISKGKNTLTIDAEELKLLKGIYVLKIRAHDFSSSLELINY"
     gene            461..4642
                     /locus_tag="PLI16_03985"
     CDS             461..4642
                     /locus_tag="PLI16_03985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOH83748.1"
                     /translation="MKKFLLFLLAFSALTTYAQLSTDEFLNTKITTTKTKDGVFVQRD
                     LTRYIGVVASMTNQNFYSGDEGQQWLKYFNRPHPNVTTLEKYFKRAGKEFGVPAELLM
                     VIGQVENNWTQVGPSIDKGWGIMHLVDNAYCSTLNEAAALLKLDPQILKDNALQNIRG
                     AAALIKKYGGNKKHKKTEDWFDAVKKFSGLINEELREQQARRYYDVLKNGVEAPTVWN
                     EAITIAAHPQINIDSKITKNTNPAKSSDYSPAISDFISCNYTTGRGGVDIDTWVHHYV
                     GVGTYAGAISWFHNCSAEASAHFVIRSSDGQITQCVAVANKAWHCGASGYPLNNSRSI
                     GVEHEATASNPSLWNSTPMLQASATMVCYFADLYDIPASFHNSPGICGHQEMPGTSTS
                     CPGAMPWTTWINYFNACSGTAPTNPTNLATSQSACPDDNVTFSWTNSGTGWHIDVSTS
                     SSFSTYYWKFVSGLTSYTGPTGFVDHADGVTPLVFQQGTTYYWRITCGSSTYSGPSFT
                     MHTCTTLPANPTVTVAACPATAVNFSWSNSGSGWHIDVSTSSSFSTYYWKFVSGLTSY
                     TGPSGFVDHTDGVTPWTIQDGVTYYWRVTSGSNYVPGTPFVKPACDVTAPTTVVVAGP
                     DWQSKDFITSFTDTDNSGGSGVSKKYYQVMDYNGTEWRANGMNGYFNDNFNSAVNAEW
                     QTTTGAGNWTVTSGQLEQTDESLYNTILSAAVAQTSANEYMYQWTGNITGTGTDRRAG
                     LHFFASEATSANRGDSYLVWFEADLGNCKIYKSLSNSLTLINTYPYAINAGTAYDYKV
                     TYSPSSGQIKIWINTTLVASWTDPSPITSGNYISLRTGNCDGFFDNVKVRKSRGSSVT
                     ITVGPYNNKDVRYESPDSTMEACRINTIINDVHGNWSPQAAKNVKIDWTGPVTSVNTT
                     GTWQTDDFTASFTDTDSLSGVQEQFYNVSYFDGTEWSANGANGFCRDDFDSTINPIWQ
                     IPAGGGTWEIISNKLNQTDDTNTNSNLYSPLAQTSGYTYLYHWTGTTTGSSATLKRFG
                     LHFFVDDPTQTNRGNSYLAFFRFDQNKIEIQKGTNNVLAFVDTVTYDLTPGITYDFKV
                     VYNPASGLINVYLNNILMLNWTDPSPITGGSYISLRSADCNAVYDNLRVYRSRGSSQL
                     ITVGAGANKDVMFENADTAQPSCIIYTLAIDKINNISSPGVLNANIDWTAPLCAMVND
                     GTGSDISETNSLTTLSANWMASADTNSGISRYWYAIGTTQGGTDVIGWTDNGQNTSIT
                     ESALTLSAGQTYYFSIKSENGAGLMSVCNSNGVVVIDASDIEESKHNPLVTVFPNPFG
                     DNATVSFELTCSQHIEISLVDLLGRSILLADQMFAAGKHTIEINSRSISISKGLYYLE
                     FKGDEHSWVEKVVHY"
     gene            complement(4846..>5125)
                     /locus_tag="PLI16_03990"
     CDS             complement(4846..>5125)
                     /locus_tag="PLI16_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOH83749.1"
                     /translation="GSLLVLGVFVFFILRILFIAERQRSAFSRIYGYGLASIIFFHVV
                     INIGMTIGLAPVVGIPLPFISYGGSSLLAFTLLLFVFIKQDASRMQVL"
     assembly_gap    5126..5135
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5136..6116)
                     /gene="rodA"
                     /locus_tag="PLI16_03995"
     CDS             complement(<5136..6116)
                     /gene="rodA"
                     /locus_tag="PLI16_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOH83750.1"
                     /translation="MSTSRNTKANIDWVLIALYVFFIFIGLMNIYSAGYSEEHKAFFD
                     FSQEHGKQMIWMAAALLVALCVMVIDARFFSTFAYIIYGLAILLLLITLFIGKEIHGS
                     CSWIVITESVRFQPLELAKFATNLALAKFLSSIDIRIQDLSTRVKAFAMLAVPVIIIL
                     LQNETGSMLVFAAFIFVLYREGLSGNVLIIGFAMIFLFIFSLLINKFIVSGFILAVAV
                     LLFFFIRRSRKNIMNYSLIVAGIIAFVFSVNLFYEHALQPHQQKRIDVLLGKISDVKD
                     AGYNVNQSKIAIGSGGFFGRGFLHGTQTKFKFVPEQNTDFIFCTIGEEWGF"
     gene            complement(6113..>6582)
                     /locus_tag="PLI16_04000"
     CDS             complement(6113..>6582)
                     /locus_tag="PLI16_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH83751.1"
                     /translation="TPLQLANFAVVIANRGYYYTPHIVKAIGKETNYTKKFLKKHKAT
                     VDPHYFDYIVEAMYAVVEGGTAHNVRMDSIKVCGKTGTAQNAGKNHSIFIAFAPKDNP
                     QIAIAAVVENSGYGATWAAPVVSLMIEKYLTRKVKRPDLEKEIMESKLIDNNQ"
BASE COUNT         1953 a         1623 c         1381 g         1615 t
ORIGIN      
        1 atggacgaat aacgcgctga acacttcggt tacaaaaaac ggcttgtcgc ttatcaatgg
       61 gcagacttat tacttaagcg taaaaactga aaacggagca ggattgaaaa ccatttgcag
      121 ttctgatgga gctattttgt ttacgcctac cggcatttcg gaaaacgctg acgaaagcgt
      181 tatttttgcg tctccgaatc cgtttaacaa gaccaccgtg ataacctact tttcagccag
      241 cgaacgcgaa atcaccgtaa tactaaccga tatgtatgga agagaattag tgttatcgga
      301 aaaaactatt tccaaaggca aaaacacgtt aacgatagat gctgaagaac tcaagctgtt
      361 aaagggaatt tatgtattaa aaatacgcgc acacgacttt tcatcttcat tggaattaat
      421 caattattaa tagttttatg ctaccaaata tttttcgaat atgaaaaaat ttttattgtt
      481 tcttttggca ttttcagcct taactaccta tgcacaactg agcacagatg agtttctcaa
      541 cacaaaaatt accacgacca aaaccaaaga tggcgttttt gtgcaacgcg acctgacacg
      601 ctatattggt gttgtagcca gtatgacaaa ccaaaatttt tattccggcg atgaagggca
      661 gcagtggctt aaatacttta accgacctca ccccaatgtt actacgctgg aaaaatattt
      721 taaaagagcc ggcaaagagt ttggcgtgcc cgccgaactg ctcatggtta tcggccaggt
      781 tgaaaacaac tggacacagg taggcccctc catagacaag ggatggggca ttatgcacct
      841 ggtggacaat gcctattgct ctacgcttaa cgaagcagcg gcgttgctaa aactggaccc
      901 gcaaattctt aaagacaacg ccctgcaaaa catccgcggg gcggcagcac tcatcaaaaa
      961 atatggcggg aacaaaaaac ataaaaaaac cgaagactgg tttgatgccg tgaaaaaatt
     1021 cagcgggctg attaatgaag aactccgcga acagcaggct cgacgttatt atgatgtatt
     1081 gaaaaatggt gttgaggctc ctaccgtatg gaacgaagcc ataacgattg cagcccaccc
     1141 tcagattaat attgactcaa agattacgaa aaacactaac cccgccaaat catctgacta
     1201 cagcccggcc atttctgatt ttatttcctg taattacacc accggcagag gcggagtgga
     1261 cattgacacc tgggtacatc attatgtggg ggtgggcacc tatgccggcg cgatctcctg
     1321 gtttcacaac tgcagcgctg aagccagcgc tcactttgta atacgttcat ccgatgggca
     1381 gataacacaa tgtgttgccg tagccaacaa ggcctggcac tgcggcgcca gcgggtatcc
     1441 cttgaacaac agccggtcga taggcgttga acacgaagca acggcatcca atcccagcct
     1501 ctggaacagc acgcccatgc tacaggcttc ggcgacaatg gtttgctatt ttgccgacct
     1561 gtacgacatc ccggcatcgt ttcacaactc acccggcatt tgtggacatc aggaaatgcc
     1621 cggcacatca acatcctgtc ccggcgctat gccctggacg acctggataa actattttaa
     1681 cgcatgcagc ggcactgccc ctaccaatcc tacaaatctg gccacctcac aatctgcatg
     1741 tcccgacgac aatgttacct tttcatggac taattctgga accggctggc atattgacgt
     1801 ttcaacaagt tccagttttt ccacctatta ctggaaattt gtatcaggac ttacttccta
     1861 cacaggacca accggttttg tggaccatgc cgacggagtt accccccttg tttttcagca
     1921 aggcactact tattactgga gaataacatg tggctccagc acttatagcg ggccgtcttt
     1981 tactatgcac acctgcacta cgctaccggc aaacccaacg gttacagttg ccgcgtgtcc
     2041 tgccacggct gtaaattttt cctggtcaaa ttcgggcagc ggctggcata ttgatgtttc
     2101 gacaagctcc agtttttcta cttattactg gaaatttgtt tcgggactta cctcctacac
     2161 cgggccttcc ggttttgttg accataccga cggggttacc ccatggacca tccaggacgg
     2221 agtaacttac tattggaggg tgacttcagg atcaaactat gtgccgggaa caccatttgt
     2281 taaacccgcc tgtgatgtta ccgcaccaac caccgttgtt gtagcagggc ctgactggca
     2341 atccaaagat ttcatcacct cttttaccga taccgacaat tcgggcggaa gcggggtaag
     2401 caaaaaatat taccaggtga tggactataa cggcaccgaa tggcgcgcta acggaatgaa
     2461 cggatacttc aacgataact tcaacagtgc ggtaaatgcc gaatggcaaa cgaccaccgg
     2521 cgcaggcaac tggacagtaa ccagcggaca gctggaacaa accgatgaat cgttatacaa
     2581 tacaatcctt tcggcagcag tggcccagac atcggcaaat gaatacatgt accaatggac
     2641 gggtaatata accggcacag gcaccgacag acgcgcaggg ctacactttt ttgccagcga
     2701 agccacctcc gctaaccgcg gagactccta tctggtatgg tttgaagccg acctcggcaa
     2761 ctgcaaaatc tataaatcac tttcaaactc gctgacgttg atcaatacct atccatacgc
     2821 tatcaacgca ggaactgcct atgactataa agttacctac agcccgtcat cagggcaaat
     2881 caaaatatgg ataaatacta ccctggttgc ctcgtggaca gatccttcgc ccattacctc
     2941 aggcaactat atctcgctgc gaaccggtaa ttgtgacggt ttttttgata atgtaaaagt
     3001 gcgcaaaagc cgcggcagca gcgttactat cactgttggg ccttacaaca acaaagatgt
     3061 gcgctacgaa agtcccgatt ctaccatgga agcatgcagg ataaacacaa taataaacga
     3121 tgtacacggc aactggtcac cacaggcagc caaaaatgta aaaattgact ggacagggcc
     3181 tgtaacaagc gttaacacaa ccggaacatg gcagacggac gattttaccg catcatttac
     3241 agatacagac tccctgtcag gtgtccagga acaattctat aatgtgagtt attttgatgg
     3301 cactgagtgg agcgccaacg gggctaacgg tttttgccgc gatgattttg acagcaccat
     3361 caacccaatc tggcaaatcc cggccggagg cggcacctgg gaaatcatca gcaacaagct
     3421 caaccagacc gatgatacta atacgaacag caatctttac agcccgttag cccaaacttc
     3481 agggtacacc tacctgtatc actggacggg cactaccacg ggcagcagcg ccaccctaaa
     3541 acgtttcgga ctgcactttt ttgttgatga ccccacgcaa acaaaccgcg gtaattccta
     3601 ccttgcattt ttccgtttcg accaaaacaa gatagaaatt cagaaaggaa caaataacgt
     3661 gcttgcgttt gtcgatacgg ttacttatga ccttacaccc ggaatcacct atgactttaa
     3721 ggttgtgtac aatcccgcct caggattgat caatgtttac ctgaataata tcctcatgct
     3781 taactggaca gatccttctc ccatcactgg cggttcttac atttcattac gcagcgccga
     3841 ctgcaatgca gtatatgaca acctcagggt ttaccggtca cgcggctcgt cgcagcttat
     3901 tacagttggg gcaggagcca acaaggatgt tatgtttgaa aacgcggata ccgctcaacc
     3961 ctcatgcatc atttatacat tagcaattga taaaatcaat aatatttctt cccctggtgt
     4021 tttaaatgcc aacattgatt ggacggcacc tctgtgcgct atggtaaatg acggtaccgg
     4081 cagcgatatc agcgagacaa attctcttac caccctttcg gccaattgga tggcctctgc
     4141 cgacaccaat tcaggaattt ccaggtattg gtacgccata ggcaccacac agggaggaac
     4201 ggatgtgata gggtggactg ataacggaca aaacacctcc attactgaat cggcattgac
     4261 actgtcagcc ggacagacct attatttcag cataaagtcc gaaaatggcg ccggtctgat
     4321 gtctgtgtgc aattcgaatg gagtagtggt tatagatgct tctgacatcg aagaaagcaa
     4381 gcacaatccg cttgttacgg tcttccccaa tcctttcggc gacaacgcca ccgtgtcgtt
     4441 tgaattaacg tgcagccaac atatagaaat ttcgcttgtt gaccttcttg gccgaagcat
     4501 actattggcc gaccagatgt ttgctgccgg caaacacacc atagagatca attcaagaag
     4561 cattagtatt tcaaaaggcc tatactatct tgaatttaaa ggtgacgaac attcgtgggt
     4621 tgaaaaagtg gttcactatt gacccggatt cggaataagg atattgtgtt tttataccaa
     4681 aatgaaatat caatacgtcc tatgggacta attgatattt cttatttggt tatgccgatg
     4741 gtcaaaataa atagtgcaaa tgaatgaagc gaagtgtgga ctataacatc tgttgcatcg
     4801 gcactatacc ataaggcaat acatatatta aatttctaac aggtattaca gcacctgcat
     4861 cctgctggca tcctgtttta taaacacgaa caacagcagg gtaaaagcca gcagcgaaga
     4921 accgccatag cttataaaag gcaaaggtat ccctacaacg ggagctaatc cgatggtcat
     4981 cccaatattt atcaccacgt gaaaaaatat tatcgaagcc aggccataac cgtaaattcg
     5041 gctaaaagca gaccgttgtc tttctgcaat aaaaagtatg cgaaggataa aaaacacaaa
     5101 aacacctaaa accagcaggc tgcccnnnnn nnnnnaaagc cccattcctc tccgatagtg
     5161 caaaaaataa aatcggtatt ctgttccggt acaaatttga atttggtttg tgtgccatgc
     5221 agaaagcctc tgccaaaaaa accgcccgaa ccaatggcta tcttcgactg attcacatta
     5281 taaccggcat ctttcacatc cgatattttg cccagcaaca catcgatacg tttttgctga
     5341 tgcggctgca gcgcatgctc atagaataga ttcacgctaa aaacaaaagc tatgatacct
     5401 gcgacaatca aagaataatt catgatattt ttccggctcc ttctgataaa aaagaacaat
     5461 agcacagcca cggccaaaat aaaaccgcta acaataaact tattgatcaa caatgaaaag
     5521 ataaacaaaa atatcatggc aaaacctatt atcaggacgt ttcccgacaa tccttcgcgg
     5581 taaagcacaa aaataaacgc ggcaaaaacc aacattgacc cggtttcgtt ttgcaacaag
     5641 atgatgatta ccggcaccgc cagcatggca aaggctttca cgcgcgtaga caagtcctgt
     5701 atcctgatat ctatactgct caaaaatttc gccagcgcca ggttcgtggc aaatttggca
     5761 agctccagag gctgaaacct caccgattcg gtaatgacaa tccacgagca ggagccatgt
     5821 atctccttgc cgataaacag ggttatcagg agcaagagta tggccagccc ataaatgata
     5881 taggcaaaag tggaaaaaaa cctggcatca ataaccatta cgcataatgc cactaacagc
     5941 gcagcagcca tccatatcat ttgtttgccg tgttcctgcg aaaaatcaaa aaatgcctta
     6001 tgctcctcag aataaccggc tgaataaata ttcatcagtc ctatgaagat aaaaaacaca
     6061 tacagcgcta tcagcaccca gtctatattg gccttggtat ttcgcgatgt gctcattggt
     6121 tattgtcaat aagtttcgac tccatgattt ccttttccag gtcgggccgt ttcaccttgc
     6181 gggtaagata cttttcaatc atcaggctga ccaccggcgc agcccacgta gcgccataac
     6241 ccgaattttc gactacggcg gctatcgcaa tctgcggatt atctttcggg gcaaaagcaa
     6301 taaatataga gtgatttttg ccggcattct gagccgtacc cgtcttcccg cataccttga
     6361 tgctgtccat ccgcacatta tgtgccgtac cgccttctac cacagcgtac atggcctcga
     6421 cgatataatc gaaatagtga ggatctacgg tggccttgtg ctttttcaga aattttttag
     6481 tataattagt ctcttttcca atggctttta caatgtgcgg cgtgtaataa taacccctgt
     6541 tggcaataac gaccgcgaaa ttggcaagct gcaaaggcgt aa
//
LOCUS       DAOSFP010000093         6579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10205_length_6579_cov_4.675506, whole genome shotgun sequence.
ACCESSION   DAOSFP010000093 DAOSFP010000000
VERSION     DAOSFP010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10205_length_6579_cov_4.675506"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..668)
                     /locus_tag="PLI16_04005"
     CDS             complement(132..668)
                     /locus_tag="PLI16_04005"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOH83752.1"
                     /translation="MKKEITLQQVKDSIRNVPDFPKPGILFKDITTALKDPDIFNYIV
                     DTIAGYYKNSGITKVVGIESRGFIMAGALAARLNAGFVPIRKPGKLPAPTFSKSYTLE
                     YGENILHIHQDALEKDDVVLLHDDLLATGGSARAASELIRLFPIKKLKINFICELGFL
                     NGKSIIDDDIEVFSLVTY"
     gene            complement(665..1699)
                     /gene="rlmN"
                     /locus_tag="PLI16_04010"
     CDS             complement(665..1699)
                     /gene="rlmN"
                     /locus_tag="PLI16_04010"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOH83753.1"
                     /translation="MKNLSQEELVVFIKNAGEQAFRAKQISEWLWRNRCVSFDEMTNI
                     SASLREQLKEAFICSSIQSFDEYVSADGTRKYTLLLTDGNIIECVLIPSDTRVTACIS
                     SQAGCPLACEFCATGQLGFSRSLDTSEIHDQIMLLMNFSKEKYQTELDNIVVMGMGEP
                     LLNYNNVMSALGKISDKNLLNFSPQRITLSTAGIVEGIKQMADENSPFHLALSLHSAI
                     EHKRKSMMPIARKYTLEQLIEALRYYHSKTNNRITIEYILFDKLNDTLEDAEALAAFC
                     RNVPVKINIISYNATAGNIYRKSKAAATTAFINFLKNKNIIVNLRASKGNDIMAACGQ
                     LAMNKNKNKL"
     gene            complement(1726..2748)
                     /locus_tag="PLI16_04015"
     CDS             complement(1726..2748)
                     /locus_tag="PLI16_04015"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOH83754.1"
                     /translation="MNNISEFIRNIPKAELHLHIEGTFEPEQMFEFAARNYITLEYHN
                     IEELRNAYQFSNLQEFLDIYYTGAYVLRSEQDFYEMTMAYLKKAAGQNIVHTEIFFDP
                     QTHLHNGIPFEVFFNGICKAVTDAEKMHGIHTGIIMCFLRHLSEESAFNTLDLSLPFK
                     DKILGVGLDSSEVGNPPEKFSRVFAKARDLGFLTVAHAGEEGPAAYIREALDLLKIDR
                     IDHGNNSKDCELLMNEIAERRIPLTMCPLSNLKLAVVKDLKDHPLKKFLDKNIAVTIN
                     SDDPAYFGGYLNENLIAIQQALDLTHDDIVTLVKNSFEASFISDEQKEKYLQSVDNFV
                     AEFVNN"
     gene            complement(2821..3366)
                     /locus_tag="PLI16_04020"
     CDS             complement(2821..3366)
                     /locus_tag="PLI16_04020"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HOH83755.1"
                     /translation="MKKSAFISVFVILSAFFVMPAISQTATMPIDEDTKLITYKEVVW
                     QEGESAKLYTTAISWINANYKNPNEVTSVREPENGKLVLNPRFKIFNVDKKGVQTDGG
                     IIKYTLKLEFKPNRYRYVFTDFNLQAVSKYPLERWLDKKDPQYTPACDNYLYQVDTTV
                     NGIIKSLKKGMVPKVTKVDEW"
     gene            complement(3475..5199)
                     /locus_tag="PLI16_04025"
     CDS             complement(3475..5199)
                     /locus_tag="PLI16_04025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH83756.1"
                     /translation="MDGKRLFFLIIFLLNSTWFAFSQKIIPKNYFIPPLDIKPYVSGT
                     FGELRPDHFHSGLDFATQNKTGLNVYCIADGYVSRIKVAAGGYGKVIYITHPNGYVSV
                     YAHLNEFNVVVEDYVKRKQYEKKSFEIELFPNRSLFHFKKGDIVGYSGNTGSSSGPHL
                     HFEIRNEITERPINPRLFGYPIPDETSPGIFTVCLYPKSPGSSVDNNKQKVCLTVEKK
                     GSSYQLAHSDTLRVSGGISFGVETYDQQYSYGKNGIYSLEILIDSTPFFKVSFDSIDF
                     SEGRYINTLIDYPEYVASRKRIIQTYISRGNRLKMYASGDNRGIYFFSDTLLHTVRII
                     ASDFNKNASSLTFPVKSVAPADSEPQTPESTLPLFRFGLKNHFENKNIILDIPSTALY
                     DSLYFEYYLKPRTKNTYSGIHQVHNSYAPLHTAATLMIKPDSIPGDTVFSKLTLARVN
                     SNYFSYVKSKQTNGYFSAKIRQFGNYAIVADTTAPVINPIGNLKDRKILSGATISFKI
                     SDDFSGINTYTLTINSAWVLAEYDPKKNLLTYIADPQHFTSGINNLELNVTDNLNNKT
                     VFKTTLLF"
     gene            complement(5266..6306)
                     /locus_tag="PLI16_04030"
     CDS             complement(5266..6306)
                     /locus_tag="PLI16_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83757.1"
                     /translation="MKKYVLSALIMLLLTLPALSQDKTSVLNKYSPSIAVIVSDAVPV
                     GYGFFVTNNILVTTINTIGRFKNAQVLLNNGKYYDILGYTASDIDYDLVLLKINYDSA
                     SPVILNSLNPVIGQKVFLVNKTNENLLELVDATLQDIKDFGYVKLLSVQAADQIKICG
                     MPVFALSGNVIGMSVMPIVDNPGINFSIPAEKIEKLLASQDELKKLILLMPLYEDIKT
                     RSVTNGDKSVAVKEFLDQGLLRYKNRDYKGAIEKYNMVIRISPNDADAMVFRGQAKYM
                     LMQYNDAMEDFNKAIILQPEFAEAYDLRGLCKAELGDIDGACDDWWQSFEKGYNPAFK
                     LLENFCDLEKMK"
BASE COUNT         1792 a         1219 c         1501 g         2067 t
ORIGIN      
        1 aatttgtcag cagcattgtc caatttttcc aaattaattt tctctatttt cagcatagtt
       61 gtttttttat acaaatgtat ataaaaatta tgtagcaaac aagtaaatta tttgttttca
      121 ctactttata cctagtaggt taccagagaa aatacttcta tatcgtcatc aataatagat
      181 ttgccattta aaaatccaag ttcacagata aagttaattt ttaatttttt tattgggaaa
      241 aggcgtatga gttccgatgc agcccgggcc gaacctccgg tggcaagcaa atcatcgtgt
      301 aagagcacca catcgtcctt ttcaagggcg tcctggtgta tatgcagaat gttttcgccg
      361 tattccaggg tatatgattt tgaaaatgta ggggcgggga gtttcccggg tttgcggata
      421 ggcacaaaac cggcgttaag cctggcggcc agcgctcctg ccataataaa gccacgcgac
      481 tctatcccca ctactttggt aatacctgaa ttcttgtaat agccggcgat agtatccact
      541 atgtaattga aaatgtcagg atctttcagc gcggtggtta tatctttgaa aagtattccc
      601 ggcttgggaa aatcaggtac atttctgatg gagtccttga cttgctgtaa cgtgatttct
      661 tttttcataa tttatttttg tttttattca tggccagttg gccgcaggca gccataatgt
      721 cgttgccttt gctggcacgt aaatttacaa taatattttt gtttttcagg aaatttataa
      781 aggcggttgt tgctgctgct ttgcttttac ggtaaatgtt tcctgcagtg gcattgtagg
      841 aaatgatatt gatttttacc ggaacattcc ggcaaaatgc cgccagggct tccgcgtctt
      901 caagagtatc attgagtttg tcaaacaaaa tgtactcaat agtgattctg ttgttggttt
      961 tgctgtgata atatctcaat gcttctatca gttgttccag cgtgtatttt ctggctatgg
     1021 gcatcatcga ttttcttttg tgttcgatgg ccgaatgcag cgacagggcc agatggaaag
     1081 gactgttttc gtcggccatt tgctttatgc cttctacgat accggctgtg gacagggtga
     1141 tacgttgcgg ggaaaaattc agaaggtttt tgtctgaaat ttttccgagg gctgacatca
     1201 cgttgttata attcagcagt ggctctccca tgcccataac cacaatatta tccagttcgg
     1261 tttggtattt ttcttttgag aaattcatca gcaacattat ctgatcatgg atttcgctgg
     1321 tgtcgaggct tctgctgaag ccgagttgtc cggtagcgca aaattcgcat gccagcgggc
     1381 atcctgcctg gcttgagatg caggctgtta cccgggtatc ggaagggata agcacacatt
     1441 cgatgatgtt gccgtcagtg aggagcaggg tatattttcg ggtgccgtcg gctgacacat
     1501 actcgtcaaa agattgaatg gagctgcaaa tgaacgcctc tttcagttgt tcgcgaagcg
     1561 aggcggaaat gtttgtcatc tcgtcaaacg atacacaacg gttacgccag agccattccg
     1621 aaatttgttt agcccggaag gcttgttccc cggcgttttt aataaaaaca accagctcct
     1681 cctgggagag atttttaata tgttttttgt tatttgtcag gaaaatcagt tgttaacaaa
     1741 ttctgctacg aaattatcca ccgattgcag gtatttttct ttttgctcat cgctgataaa
     1801 ggaagcttca aaagagtttt ttaccagggt tacgatatcg tcgtgggtga ggtcaagcgc
     1861 ttgttggatg gcgatcaggt tttcgttgag gtagccccca aaataagcgg ggtcgtcgga
     1921 gttaatggta acagcaatat ttttgtcaag aaactttttg agggggtggt ctttcaggtc
     1981 ttttactacg gctagtttga ggtttgacag ggggcacatc gtcaggggta ttcgtctttc
     2041 agcaatttca ttcatcaaca gttcgcagtc tttggagttg ttgccgtggt cgatacgatc
     2101 tattttcaac aagtccagcg cctctctgat gtatgccgcc gggccttctt caccggcatg
     2161 ggccacggtt aagaatccca ggtcacgggc tttggcaaat acacggctga atttttcggg
     2221 aggatttcct acttccgagc tgtccaatcc cactccgaga attttatctt taaatggcaa
     2281 cgacaggtct aaggtgttga atgcggactc ttcgcttaaa tggcgcagaa aacacatgat
     2341 gatacctgta tgtatgccgt gcattttttc agcgtctgtt acagctttgc aaattccgtt
     2401 gaaaaacact tcgaatggaa tgccgttgtg caaatgtgtc tgcgggtcga aaaatatttc
     2461 ggtatgtacg atgttttgtc cggcagcttt tttcaggtat gccatcgtca tctcgtaaaa
     2521 gtcctgttcg ctgcgcaata cgtaagcacc ggtataataa atatcaagga actcctgcag
     2581 gttgctgaac tgataagcgt ttcggagctc ttcaatgttg tggtattcga gcgtgatata
     2641 attacgggcg gcaaactcaa acatttgttc aggttcaaaa gtgccttcga tgtgcagatg
     2701 aagttctgcc ttggggatat tgcgaataaa ttcagagata ttgttcatag gtcttagttt
     2761 gtacaaagat aaaaaaagcg gatgtaaaat ccgctaagta ctgacaaaaa atatgaaaaa
     2821 tcaccactca tcaaccttcg tcaccttggg aaccatacct tttttgaggc ttttaataat
     2881 tccgttaacg gtggtatcta cctgataaag ataattatcg caggccggag tgtattgggg
     2941 gtcttttttg tcgagccagc gttccagggg atattttgat acggcctgaa ggttgaagtc
     3001 ggtaaaaaca taacgatatc tgttgggttt aaattcaagc tttaacgtgt atttgatgat
     3061 tccaccgtcg gtttgcacac cttttttgtc cacattaaat atcttaaaac gcgggtttag
     3121 caccagtttg ccgttttcgg gctcccgcac ggaggtaacc tcgttggggt ttttgtaatt
     3181 ggcgtttatc caggaaatgg cggtagtata taacttcgca gattcgcctt cctgccacac
     3241 gacttcttta taggtaatca atttggtgtc ctcatcaata ggcatggtgg cggtttgcga
     3301 aatggcgggc attacaaaaa atgccgaaag aataacaaat acagaaatga aagccgattt
     3361 tttcatgatg tcaaatttta aaacaaaaat aaattttcct ggagtattcg caataatcaa
     3421 accaaatttt taataacaga attcgacggg aatttttcta ttacgtaatt cgggctaaaa
     3481 aagcagggtt gttttaaata cggttttatt gttgaggttg tcggtaacat tgagttcaag
     3541 attgtttatg cctgatgtaa agtgttgcgg gtcggcaata taggtcaaca gattcttctt
     3601 cgggtcgtat tcggcaagca cccacgcgct gttaatggtg agggtgtagg tattgatgcc
     3661 tgaaaaatca tcagaaattt taaaggaaat tgttgcgccc gataaaattt ttctgtcctt
     3721 caggttgccg atgggattaa ttacaggggc agtggtgtcg gccacaatgg cgtaattgcc
     3781 gaactggcgt attttggcag aaaaatatcc attagtttgt ttacttttta cataagaaaa
     3841 ataattactg tttacacggg caagagtgag ttttgaaaaa acggtatcgc cggggatgct
     3901 gtcgggtttt atcatcagtg tggcggcagt gtgcaggggc gcataagaat tatgtacctg
     3961 atgaataccc gaataggtgt ttttggtgcg gggtttcagg tagtattcaa aataaagtga
     4021 atcatacagg gcggtggagg gtatgtcgag tatgatattt ttgttttcaa aatgattttt
     4081 aagtccaaaa cggaataggg gcagggtgct ttccggagtt tgtggttcgc tgtcagccgg
     4141 cgcgacagat ttgaccggga atgtgagtga tgacgcattt ttattaaaat ctgatgcgat
     4201 tatcctgacg gtatggagca gggtgtcgga aaaaaaataa atgccgcggt tatcgccgga
     4261 ggcatacatt ttaagcctgt ttccccggga gatataagtt tgtattattc ttttacggct
     4321 ggccacatat tcaggataat caatcagggt gttgatgtag cggccttcgc tgaaatctat
     4381 ggaatcaaaa gaaactttga aaaaaggagt gctgtcgatc aggatttcca gcgaataaat
     4441 gccatttttt ccatacgaat actgctgatc ataggtttct acaccaaacg agatgccgcc
     4501 cgatacccgc agtgtatcac tatgggcaag ctgatatgaa ctcccctttt tttccacggt
     4561 taagcaaact ttttgtttgt tgttatccac gctgctgcct ggtgatttgg ggtacaaaca
     4621 aacggtaaat ataccgggac ttgtttcgtc ggggatggga tagccgaaaa gtctcggatt
     4681 tataggccgt tcggtgattt cgttgcgtat ttcaaaatgc agatgcgggc cggatgaaga
     4741 gcctgtattc cctgaatagc cgacaatatc accttttttg aaatgaaaca gagaacggtt
     4801 gggaaaaagt tctatttcaa aggatttttt ttcatattgt ttgcgtttta cgtaatcttc
     4861 aaccactaca ttgaactcgt tgaggtgggc ataaaccgat acataaccat tcggatgggt
     4921 gatgtaaata acttttccgt agccgccggc agcaactttt atccgtgaaa catagccatc
     4981 ggcgatgcaa taaacattaa gtccggtttt gttttgtgtg gcaaaatcaa ggcccgagtg
     5041 aaaatgatcg gggcggagtt caccgaaagt gcccgacaca taaggtttga tatcgagtgg
     5101 cgggataaaa tagtttttag gaataatttt ttgggaaaat gcaaaccatg tgctgttcag
     5161 caggaagata atcagaaaaa aaagccgttt gccatccaca ggaataaaag attttttggt
     5221 cggaactatg ataatgatct tttataaacg gcagtaaatt gttttttatt tcattttttc
     5281 caggtcgcag aaattttcga gcaatttgaa ggcggggtta taaccttttt cgaaagattg
     5341 ccaccagtcg tcgcatgcgc cgtctatatc gcccagttct gccttgcaaa ggccgcgcag
     5401 gtcgtatgct tcggcaaact cgggctgcaa aatgatggct ttgttaaaat cttccatagc
     5461 atcgttgtat tgcataagca tatatttagc ctgccctctg aaaaccatgg cgtcggcatc
     5521 atttgggctt atgcggatta ccatattgta tttttcaatg gcgcctttat aatctctgtt
     5581 tttgtacctc agcaagccct gatccaaaaa ttcttttact gccacacttt tgtctccatt
     5641 ggtcacggag cgcgttttaa tatcttcata aaggggcatt agcagtatga gtttttttaa
     5701 ttcatcctgt gaggctaata gtttttctat tttttcggct ggtatagaga agttgattcc
     5761 cgggttatct actattggca ttacagacat cccgatgaca ttacccgaaa gcgcaaaaac
     5821 cggcatgccg caaattttta tttgatcagc ggcttgcact gataatagct ttacatagcc
     5881 gaaatcttta atatcctgca aggtggcatc caccaattca aggaggttct cattggtttt
     5941 gtttaccaga aaaacctttt gaccaattac cggattcagg gagtttaata tgacgggact
     6001 ggcactgtcg taatttattt ttagaagcac cagatcataa tcaatatctg aggcggtata
     6061 tccgagaata tcatagtact ttccgttgtt taacaatacc tgcgcatttt tgaagcggcc
     6121 aatagtattg atggtggtaa ctaaaatgtt gttggtaaca aaaaaaccgt aacctaccgg
     6181 cacggcatca gatacgataa cagcaatgga cggagaatat ttattcagta ccgatgtttt
     6241 gtcttgtgat agtgccggca aggtcagcaa cagcattatt aaggcgctta aaacatattt
     6301 tttcataaaa tttcacacaa aattatatta aataaataaa actatgaaaa acacaagtta
     6361 ataattactg acaaatttaa ttaaaaaaga agcacggctg tgtttttttt gagttggaag
     6421 cgaaaaagat gaaaaatgtg gaacaagttt atatgaattc agttttttaa gttgtcttgt
     6481 ttgagagaga ttctattagc aggtattaat ttttttttgc ataataaacc taaaaaaaat
     6541 cgttttaacc taaaaaaagg tatagaaaat gttttaaaa
//
LOCUS       DAOSFP010000094         6579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10206_length_6579_cov_4.571122, whole genome shotgun sequence.
ACCESSION   DAOSFP010000094 DAOSFP010000000
VERSION     DAOSFP010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10206_length_6579_cov_4.571122"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLI16_04035"
     CDS             <1..390
                     /locus_tag="PLI16_04035"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOH83758.1"
                     /translation="LSTINQAHQGEGRIITVVGAGGDRDKTKRPEMGKIAAEMSQQVI
                     LTSDNPRTEDPEKILDDIMSGIGITEKKKVLVITNRKEAIKTAYVMAQPGDIILIAGK
                     GHEKYQEIMGVKHPFDDKQIIKDLMDK"
     gene            392..1651
                     /gene="mraY"
                     /locus_tag="PLI16_04040"
     CDS             392..1651
                     /gene="mraY"
                     /locus_tag="PLI16_04040"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOH83759.1"
                     /translation="MLYYLFDYLDKQFNFPGAGVFQYISFRAAMSIITSLFISLIIGK
                     RLIRYLQKKQVGEVVRDLGLQGQIEKQGTPTMGGIIILSAILVPTLLFAKLHNVYIIL
                     MIITTIWLGTIGFIDDYIKVFKKNKKGLAGKFKILGQIGLGLIVGLTMYYNDSVVIRE
                     KANRPAAKEVSTENIFKMENFSPSTKVYSEKPVKSTKTTIPFFKNNEFDYGSLLSWLG
                     DGYQKWAFLIFIPIIIFIIVAVSNGANLTDGLDGLATGTSAIIGVTLAVLAYVSGSVV
                     FANYLNIMYIPNTGELVIYISAFVGACIAFLWYNSYPAQIFMGDTGSLAIGGIIAVFA
                     IIIRKELLIPLLCGIFLAQSLSVIMQVSYFKYTKKRFGEGRRIFKMSPLHHHYQKVGY
                     HEAKIVMRFFIVGIMLAILTIVTLKIR"
     gene            1666..3006
                     /gene="murD"
                     /locus_tag="PLI16_04045"
     CDS             1666..3006
                     /gene="murD"
                     /locus_tag="PLI16_04045"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOH83760.1"
                     /translation="MQTRIVILGGAESGIGAAILAKKKGFEVFVSDAGEIKKNYKDVL
                     LHHAISFEEGKHTENLCLRAQEVIKSPGIPDNAPVIRKMKEHGVPVISEIEFAARYTE
                     AKKICITGSNGKTTTSMLTFHILKKAGLHVGLAGNIGKSFAWQVAENDFDYYVLEISS
                     FQLDYMFRFKADVAVITNITPDHLDRYQNRFQDYIDAKFRILRNQGAGDFFIYCADDP
                     VVGQELGRRSITSSKIPFSLAEPPDGKGAYMKDNKLNINILSNKITMGVENFALPGKH
                     NLYNSMAAGIISRIFDIRKDVIKESMSEFQGVEHRLEFVTKVHGIEFINDSKATNVNS
                     TWYALESMTHPVIWIVGGVDKGNDYSCLKPLVKDKVKAIICLGTDNDKLIKEFGDLVS
                     EVKEARSAEEAVQISYALGNPGDVVLLSPACASFDLFNNYEDRGMKFKRAVYDL"
     gene            3019..4224
                     /locus_tag="PLI16_04050"
     CDS             3019..4224
                     /locus_tag="PLI16_04050"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOH83761.1"
                     /translation="MKKFFQNFKGDKVIWAVVVIIALFSILAVYSSTGTLAYRSKKAA
                     GNTEFFIMKHFFTLIMGIALMYVSHLIKYTHYSKISQILVFIAIVLLPITLFTGSSVN
                     EASRWLTLPIIDLKFQTSDLAKLALIMYLAREISRRQEKMDDIKTGFLPIVIPIVLIC
                     VFILPANLSTAAILFMTCVILMIVGRVKIKYIVYLFGIMLVAGGLLVLILKNTPYESR
                     VSTWTNRLATYFGEEKEQNPDDVYQITQSKIAVAQGGFFGKFPGNSTQRNVLPQAYSD
                     FIYAIILEEYGFLGGLVVMLLYMILLYRGIRLATKCDKIFGALLGLGVSFAIVFQAVI
                     NMGIVVGLFPNTGQPLPLISMGGTSIWFTSIGIGILLSISRANDESEKQRQEAAQNND
                     LKTVTADAN"
     gene            4214..5320
                     /gene="murG"
                     /locus_tag="PLI16_04055"
     CDS             4214..5320
                     /gene="murG"
                     /locus_tag="PLI16_04055"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOH83762.1"
                     /translation="MQTKNYKFIISGGGTGGHVFPAIAIANALKKSCPDAEILFVGAN
                     GRMEMDKVPEAGYKIIGLNISGLRRSLSPQNIATLFKLLSSLQKAKKIIRQFKPDAVI
                     GVGGYASGPVLKIAASKKIPTLIQEQNSYPGITNKILSKKVNKICVAYEGMEKFFPKE
                     KIILTGNPVRQDMTELQGKREPGQEFFGMDTVRKTILVVGGSLGARTINNAILEKLEL
                     FKQNNIQLIWQTGKTFFETAQKACEKYKDNGINCHAFISKMDYAYAAADVIVSRAGAI
                     AISEMCLVGKPAILVPSPNVTEDHQTKNALALSENDAAILVKDAEAAEKLAAEAIALV
                     QDEARAVRLSSNIARFAHKDVADRIAGIILDMIK"
     gene            5326..>6579
                     /gene="murC"
                     /locus_tag="PLI16_04060"
     CDS             5326..>6579
                     /gene="murC"
                     /locus_tag="PLI16_04060"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOH83763.1"
                     /translation="MTSGTSKTYYFLGIGGIGMSALARFFKRQGHRVAGYDKTPTKLT
                     QQLIDEGIDVHFHENINSLPSGIDLVVYTPAVPVEHAEYRYFIENKVPLKKRAVVLGE
                     VVKNNFLIAVAGTHGKTTITGMIAHIMQSSGIPVNAFIGGIVKNFQSNLVYTENAVVA
                     VTEADEYDRSFLSLDPDIAIISAIDADHLDIYADKKNLVDSYHAFAGKVNPIGTLIIK
                     NGLPEPASHEYNLFHYDKNAAADFYAKDVYIENARYHATICLQQEEVQVVLNVPGWHN
                     VENAVAAAAACYVFGAGAAEIKQGLETFEGVSRRFDVRFQHGNTTYIDDYAHHPEELR
                     AFIGTVRELYPDRKITGIFQPHLYSRTRDFAAEFAAVLDNLDEAILLDIYPARELPIE
                     GVTSGIILEKMKIADAHEEVPVVLRN"
BASE COUNT         1896 a         1488 c         1541 g         1654 t
ORIGIN      
        1 ctgagcacta tcaaccaggc acaccagggt gaaggaagaa taattaccgt ggtgggcgcc
       61 ggcggtgacc gcgacaaaac caaacgtccc gaaatgggaa aaattgctgc cgaaatgagc
      121 caacaggtga tactgacctc cgacaacccg cgcacggaag accccgaaaa aattctcgat
      181 gatataatgt ccggcatcgg gatcaccgaa aagaaaaaag tattggttat cacaaatcgc
      241 aaagaagcca taaaaaccgc ttatgtcatg gcgcagccgg gcgatatcat ccttattgcc
      301 ggcaaaggcc acgaaaaata ccaggaaata atgggcgtga agcacccttt tgatgataaa
      361 caaattatta aagatttaat ggataaataa tatgctgtac tatctttttg actatctgga
      421 taaacaattt aatttccccg gcgccggagt ttttcaatat atttctttcc gtgccgccat
      481 gtcaattatc acttcccttt ttatttccct tattataggt aaaaggctga taaggtattt
      541 gcaaaaaaag caggtaggcg aggtggtcag agacctgggc ctgcaggggc agattgaaaa
      601 acagggcaca cccaccatgg gtggcatcat tatactcagc gccattcttg tcccgacact
      661 cttgtttgcc aaactccaca acgtgtacat catactgatg atcatcacca ccatatggct
      721 gggaaccatc ggttttattg acgattatat caaagttttc aagaaaaata aaaaaggcct
      781 ggccgggaaa tttaaaatcc tgggacaaat cggcctgggt cttattgttg gcctgaccat
      841 gtattacaac gactcggtgg ttatcaggga aaaagccaac aggcccgcgg ccaaagaggt
      901 gagcacggaa aatattttta aaatggaaaa tttcagcccc tccaccaagg tatattcgga
      961 aaaacctgtg aaatcgacaa aaaccactat cccgtttttt aaaaataatg aatttgacta
     1021 tggctcatta ctttcctggc tgggcgatgg ctatcaaaaa tgggcctttt tgatctttat
     1081 tcccattatc atatttatta tcgtggctgt atccaacggg gccaacctca ccgatggctt
     1141 ggacgggctg gccacgggca cttcggccat catcggggtt acgctcgccg tgctggccta
     1201 cgtatcgggc agcgtggtgt ttgccaatta tctcaacatc atgtatatcc ccaataccgg
     1261 cgaactggta atctatatca gcgcctttgt gggagcctgt atcgcgttcc tgtggtacaa
     1321 ctcctatccg gcgcagatct ttatgggcga taccggaagc cttgccattg gcggtattat
     1381 agctgttttt gccatcatca tccgcaaaga actgctgata cccttgctat gcggtatttt
     1441 cctggcacaa agcctttcgg taattatgca ggtaagctat tttaaatata ccaaaaaacg
     1501 atttggcgaa ggcagaagga ttttcaagat gtcgcccctc caccatcact accagaaagt
     1561 gggatatcac gaagcgaaga tagtgatgcg cttttttatt gtgggaatta tgctggccat
     1621 attgacgata gtaacattga aaatcagata gaaatgccat aaaaaatgca aacccgcatt
     1681 gtcatacttg gaggggccga aagcggaata ggagccgcaa tactggcaaa gaaaaaaggc
     1741 ttcgaagtgt ttgtatccga tgcaggtgag attaaaaaaa attataaaga cgttctttta
     1801 catcatgcaa taagttttga agaagggaaa catactgaaa acctttgttt acgggcacag
     1861 gaagtgatta aaagtccggg aatacccgac aacgccccgg taatcaggaa aatgaaagag
     1921 cacggtgtcc ctgttatttc cgagatagaa tttgccgcca gatatacgga ggccaaaaag
     1981 atttgcatca caggcagcaa tggaaaaaca accaccagta tgctcacgtt tcacatcctg
     2041 aaaaaagcgg gacttcatgt cggccttgcc ggaaacatcg gtaaaagctt tgcctggcag
     2101 gtggctgaaa acgattttga ttactatgta cttgaaatca gcagctttca actggactat
     2161 atgtttcgtt ttaaagccga tgtagccgtg attaccaaca tcacccccga tcatctcgac
     2221 cggtatcaga acaggtttca ggattacatc gatgcgaaat tcaggatact gcgaaatcag
     2281 ggtgccggcg atttttttat ctactgtgcc gacgacccgg ttgtagggca ggaattaggc
     2341 aggcgcagca tcacttcttc aaaaatacct ttttcgttgg cagaaccgcc tgacggaaaa
     2401 ggtgcttata tgaaagacaa caaattgaat attaatatat tatcaaataa aataaccatg
     2461 ggagtagaaa attttgcatt accggggaaa cacaatcttt acaattcaat ggctgccggg
     2521 attatttccc gcatctttga tatccgtaag gacgtaatta aggaaagtat gtccgaattt
     2581 cagggggtgg aacatcgcct ggagtttgtg accaaggtgc acggaataga gtttatcaac
     2641 gattcaaaag ccaccaatgt aaactctaca tggtatgccc tcgaaagtat gacacatcct
     2701 gtaatctgga ttgtgggcgg tgtggataag ggcaacgatt attcctgcct gaaacccctg
     2761 gttaaggaca aggttaaggc tatcatttgc ctgggcaccg ataatgacaa actgataaaa
     2821 gagtttggag acctggtgtc tgaggtaaaa gaagccagat cggcggaaga agccgtacag
     2881 atttcttatg ccctcggaaa tccgggagat gttgtgctgt tgtcgccggc ctgtgccagt
     2941 ttcgacctgt ttaataacta cgaagacagg ggcatgaaat tcaaaagagc tgtttacgac
     3001 ctataaaaaa tagtaaaaat gaagaaattt tttcaaaatt tcaaaggcga taaagttatc
     3061 tgggctgtag tggtcataat tgccctgttt tctattctgg cggtgtacag ttcaaccggt
     3121 acgttggcat accgcagtaa gaaagctgcc ggtaatacgg agttttttat tatgaaacac
     3181 ttttttacct taatcatggg tatagccctg atgtatgttt ctcacctgat aaaatacacg
     3241 cattactcaa agatctcgca gatattggtt tttattgcca tcgtgctgct gccaatcacc
     3301 ctgtttacgg gaagttccgt gaatgaggct tcgcgttggc tgacactgcc aatcatcgac
     3361 ctgaagtttc agacttccga ccttgccaag ctggccctga tcatgtatct cgccagagaa
     3421 atctcgcgaa ggcaggaaaa aatggacgac atcaaaacag gttttcttcc aatagtaata
     3481 cctatagtcc ttatttgcgt gtttattttg cctgccaact tatccaccgc tgccatttta
     3541 ttcatgacct gtgtcattct gatgatcgtc ggcagagtga aaataaaata catcgtttat
     3601 ctttttggta tcatgcttgt tgccggtgga cttttggtgc tcatactaaa aaatacgcct
     3661 tacgaaagcc gtgtttctac ctggaccaac cgtctggcta cctactttgg cgaagaaaaa
     3721 gaacaaaacc ccgacgacgt ctatcagatc actcaatcga agatagccgt ggctcagggc
     3781 ggtttcttcg ggaaatttcc cggcaacagc acgcaacgca acgtgcttcc gcaggcgtat
     3841 tctgacttta tttatgccat cattcttgaa gaatacggct ttttgggcgg gctggtggtg
     3901 atgctgttat atatgatttt gttgtaccgc gggatacgcc tggccaccaa atgcgacaaa
     3961 atttttggcg ccttgcttgg gctgggggtg agttttgcca tagtgtttca ggcggtaatc
     4021 aatatgggca tagtggtggg actgtttccc aatacagggc agccgctgcc gctgataagc
     4081 atgggaggta cttctatctg gtttaccagt atcggcatcg gcatcttact cagcatcagc
     4141 agggccaacg atgagtccga aaaacaacgc caggaggcag cacaaaataa tgacttaaaa
     4201 actgtaacag cagatgcaaa ctaaaaacta caaatttatc atcagcggag gcggtaccgg
     4261 aggccatgtt tttccggcga ttgctattgc caatgccttg aagaaaagtt gtccggatgc
     4321 cgaaattctg tttgtcggtg caaacggccg aatggagatg gacaaagtgc ccgaagccgg
     4381 atacaaaatc ataggattga acatcagcgg gctgcgccga agcctctcgc cgcaaaatat
     4441 cgccaccctg tttaagctcc tctccagcct gcagaaagcc aagaaaatta tcaggcaatt
     4501 caagcctgat gccgttatcg gtgtcggcgg ctatgccagc gggccggtat taaaaattgc
     4561 cgcatccaaa aaaataccca ccctcataca ggaacaaaat tcgtatccgg gcattaccaa
     4621 taaaatatta tccaaaaaag ttaacaaaat ttgtgtggct tatgaaggca tggaaaagtt
     4681 tttccccaaa gaaaaaatta tccttacggg caaccccgtg cggcaggata tgactgagct
     4741 gcagggcaag cgggaaccag gccaggaatt cttcgggatg gatactgtgc gcaaaaccat
     4801 tcttgtggtg ggcggcagcc tgggtgcgcg caccattaac aacgcgattc tggagaaact
     4861 cgaactattt aaacaaaaca atattcagct gatctggcaa accgggaaaa ctttttttga
     4921 aacggcccaa aaagcatgcg aaaaatacaa ggataacggg ataaactgcc acgcgtttat
     4981 ttcgaagatg gattatgcct atgccgccgc cgatgtgata gtgtcgcgtg ccggagccat
     5041 cgccatttca gagatgtgtc ttgtgggtaa acccgccatc ctggtgcctt cgcccaatgt
     5101 aacggaagac catcagacaa aaaatgccct ggccctgtcg gaaaacgatg ctgcaatcct
     5161 ggtaaaagat gccgaagcgg ccgaaaaact ggcagccgaa gccatagccc tcgtgcagga
     5221 cgaggcgcgt gctgtccgtc ttagcagcaa tatagcgcgg tttgcccata aagatgtggc
     5281 cgaccgtatt gccggaataa ttttagacat gattaagtga gcggtatgac aagcggaaca
     5341 agtaagacat attatttttt aggtatcggt ggcatcggta tgagcgccct ggcacgcttt
     5401 tttaaaagac agggtcatcg ggtggctggt tacgacaaaa ccccgaccaa actaacccaa
     5461 cagcttatcg atgaaggcat cgatgtgcat tttcatgaaa atatcaattc cctgccttcc
     5521 ggcattgacc tggtggtata tacgccggcc gtacccgtgg aacatgctga ataccggtac
     5581 tttatcgaaa acaaggtgcc tttaaagaaa agagccgtgg tgctgggcga ggtggtaaaa
     5641 aataattttc tgattgctgt agcaggaacc catggcaaaa ccaccattac cggcatgata
     5701 gcacatatta tgcaatcgtc cggcatacca gtcaatgcat ttataggcgg catcgtgaaa
     5761 aattttcaat ccaacctggt ttataccgaa aatgccgtgg tagccgtaac cgaagccgac
     5821 gaatacgacc gttcattttt atctctcgac cctgatatcg ccatcatttc tgccatcgat
     5881 gccgaccacc tggatattta tgccgacaaa aaaaacctgg tggattccta ccatgccttt
     5941 gccggcaaag taaaccctat cggcaccctg atcataaaaa atggcttgcc cgaaccggca
     6001 tcgcacgagt ataacctgtt tcattatgat aaaaacgccg ccgcagattt ttatgcaaaa
     6061 gacgtgtata tcgaaaatgc caggtatcac gccacgattt gtctgcaaca ggaggaggta
     6121 caagttgtct tgaacgtccc cggatggcac aatgtggaga atgcggtagc tgccgctgcc
     6181 gcctgctatg tcttcggagc aggcgctgct gagatcaaac agggattgga aactttcgaa
     6241 ggcgtgagcc gtcgctttga tgtcagattt caacacggaa ataccaccta tatcgatgat
     6301 tacgcgcatc atccggaaga gctccgcgcc tttatcggga cggtaaggga actttatccc
     6361 gaccggaaga ttaccggcat atttcagccg catttgtact ccaggacaag agattttgcc
     6421 gctgagttcg ctgctgtact ggataatctt gacgaagcga tcctgctcga tatttatcct
     6481 gcacgagaac tgcctatcga aggcgttacc tccggtatta ttcttgaaaa aatgaagata
     6541 gccgatgccc atgaagaggt cccagttgtt ttgcgcaac
//
LOCUS       DAOSFP010000095         6547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10282_length_6547_cov_3.554991, whole genome shotgun sequence.
ACCESSION   DAOSFP010000095 DAOSFP010000000
VERSION     DAOSFP010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10282_length_6547_cov_3.554991"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLI16_04065"
     CDS             <1..214
                     /locus_tag="PLI16_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83764.1"
                     /translation="KWEYAGGIIFMIIGIGLSPFIFMFNYRMNHSVWMSLGIIMAITI
                     PFAIIGFLFILSHFLKKKNRTKDKTS"
     gene            226..993
                     /locus_tag="PLI16_04070"
     CDS             226..993
                     /locus_tag="PLI16_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HOH83765.1"
                     /translation="MDTTLKQKFIELWEKYFGDAELPITFYYSEGTGDTKPAEKPTGW
                     KCLIGALAAVRKGSSLHFNAGTLGCGGALRYLGYTTKMRPDFEYFLSCGNEKMEGERY
                     KRTPGIVLEMMKNQQQLPVNGKNIVFKRWDKLTEDDQPEAVIIFARPDVISGLFTLAN
                     FDQVDNEQVIAPFGAGCSSIVHYPYLESLSDKPKAVIGTFDPSARPYVWANEISFAVP
                     FSKFEKMIGYMEESFLITETWTTLKKRMDKASVKNDQ"
     gene            complement(1060..2146)
                     /gene="gcvT"
                     /locus_tag="PLI16_04075"
                     /pseudo
     CDS             complement(1060..2146)
                     /gene="gcvT"
                     /locus_tag="PLI16_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
     gene            2338..4203
                     /locus_tag="PLI16_04080"
     CDS             2338..4203
                     /locus_tag="PLI16_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOH83766.1"
                     /translation="MHITKLNIPRWLILLIDIFICGFSVVLTYLLRFNFNVPEIESHD
                     LPYVLAFTVGIRFITFLIAKTYTGIIQYTSTGDAIRIVFVTATGSIVFVITDLITYFF
                     INGVNFIPYSIIIIEFITTTFLLAAFRILVKITYMEISNPSRDKTNVLIYGAGESGLI
                     SKRSLDRDAGTKYKVLAFIDDDAKKIGMKIEDIPIYGPDRLEKLLEDNHIEHLIISIQ
                     NIKALKKQEIIEKCMAYNTKVLSVPPVSKWINGELSFKQIKKINIEDLLERDEIRLDE
                     EKVRRELLDKCILVTGAAGSIGSEIVRQIVRFSPKKIILLDQAESPMYLLELELNEKY
                     PKANIEVIICDINHQVRMRKIMETFSPQIIYHAAAYKHVPMMENNPVEAVITNVFGTK
                     TIADLAVEFKAEKFVMVSTDKAVNPTSIMGATKRIAEIYTQSLNKKNVTRFITTRFGN
                     VLGSNGSVIPLFREQIEKGGPVTITDPEVTRFFMTIPEACQLVLEAGTMGHGGEIFIF
                     DMGKSVKILDLAKKMIKLSRLTLGKDIQIKYTGLRPGEKLYEELLNNKENTLPTYHPQ
                     IMIAKVAESDFELISKQVMELESLLDSHDNIQIVKKMKEIVAEFKSQNSVYAKLD"
     gene            complement(4293..5486)
                     /gene="sucC"
                     /locus_tag="PLI16_04085"
     CDS             complement(4293..5486)
                     /gene="sucC"
                     /locus_tag="PLI16_04085"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOH83767.1"
                     /translation="MNLHEYQAKELLKTFGVSVPEGIVANTPDEAMNAALIIGAGTGS
                     EKFAVKAQIHAGGRGKGGGVKIASGSNEVFEHARHIIGMQLITPQTKAEGKKVHKVLV
                     EQNIYYPGPQPIREFYIGILLDRHTGKNVIMYSPRGGMDIEKVAEETPEQIFKEEIHP
                     GVGIRDFQCRRIAFNLGLEGEAYKKMLPFVKNLYKAYTGCDLTLLEINPVIKTSDDKI
                     FAADAKMVADNNALFRHCIIANMRDLAEENPVEVEAMQYGLNFIKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGSPANFLDVGGTANAKRVEQAFRIILSDENVKAILVNIFGGIVRC
                     DRVANGIVDAYKSLGEIKVPVIVRLQGTNAEEAKEIIDNSGLKVFSAIKLQEAADLVN
                     KVLKD"
     gene            5614..>6547
                     /locus_tag="PLI16_04090"
     CDS             5614..>6547
                     /locus_tag="PLI16_04090"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOH83768.1"
                     /translation="MKSDYKIFCLFLFILMFCLPAAMAQKKTAYATRITAPPDIDGVL
                     TDACWRLCDSADSFRQYVPVYDVNPTQRTVVRIAYNDEAIFVGAMMYDNAPDSILTEF
                     GVRDDELNADRFAIQFDTYHMQTDAYTFAVWASGTQADWRHTDETYNAVWQSAVKIND
                     KGWACEMKISYSALRFPKTAVQEWGLQIFRGIRRHRETDQWALEVKSNANFLTDWGKL
                     EGIRDVKPALRLSLTPFVSLYGDHYPYNEKGKSNFSYSFSGGLDLKYGINESFTLDIT
                     LLPDFSQVQSDYPVKNISAQETVYEEYRPFFTEAI"
BASE COUNT         1892 a         1489 c         1375 g         1791 t
ORIGIN      
        1 gaaatgggaa tatgcgggcg gtatcatttt tatgatcatc ggcataggcc tcagcccgtt
       61 catctttatg tttaattacc ggatgaatca ttcggtatgg atgagcctcg gcatcattat
      121 ggcaattacc attccctttg ccatcatagg ctttttgttt attttgagcc attttctgaa
      181 aaagaaaaac agaacaaaag ataaaacatc atgacaatat aaaatatgga tacgacctta
      241 aaacagaaat ttattgaatt atgggaaaag tatttcggtg acgccgaact gccaataacc
      301 ttctattata gcgaaggtac cggggacaca aaacccgccg aaaaacccac cggatggaaa
      361 tgcctgattg gcgcactggc agccgtaagg aaaggctcct ctttacattt taatgcaggc
      421 accctgggct gtggcggcgc cttacggtat ctcggttata caacaaaaat gcgtccggat
      481 tttgagtatt ttctttcgtg cggtaatgaa aagatggagg gggaacgtta caagcgcaca
      541 cccggtattg tgctggagat gatgaaaaac cagcaacagt tgcccgttaa tgggaaaaac
      601 atcgttttta aaaggtggga taaacttaca gaggacgatc aacccgaagc agtgatcatt
      661 tttgcaagac cagacgtgat ctctggattg tttacattag ccaattttga tcaggtggac
      721 aacgagcagg tgatagcacc ttttggagcc ggctgtagct ctatcgtgca ctacccctat
      781 ctcgaaagcc tgtctgataa gcccaaagcc gttatcggca cgttcgatcc ttcggcaaga
      841 ccttatgtct gggctaatga gatttccttc gcggttcctt tcagcaagtt tgagaaaatg
      901 attggctata tggaagagag ttttctgatt acagaaacat ggaccactct gaagaagaga
      961 atggataagg cttccgtgaa aaatgatcag taactgaaaa ataaaaaaag ccgctccaaa
     1021 ggatagacgg ctttttaaag agttagtcaa tacggtaact tatcctttat aaaaaggtaa
     1081 tttcaccact tttgctttaa tcagtttttc acggatctta acataaatct cggtatccac
     1141 ttttgaaaaa gcagttttta cataacccat cccaacgttt ttctgcagtg atggagccat
     1201 agtacccgag gttacttcgc ctattttatt gccgtcagca tcgcaaattt cataataatg
     1261 acgggggata cctctgtcaa tcatttcaaa acccactaat ttccggctta cgccctcggt
     1321 ttttaatttc aggttatatt ctttcaataa aattcttttc atcggtgaat ttggtgatcc
     1381 agcccaaacc agcttctata ggtgaggtag tatcgttgat gtcgttgcca taaagacaga
     1441 atcccatttc gagacgcaac gtatcgcggg cagccagtcc tgttggtttt attccaaatt
     1501 cttttccggc ttccatcacg gcattccaca tcacttcggc atactgattc gggaaataaa
     1561 tttcacatcc acccgaaccg gtatagcctg tagtggagaa aatcacatca tcaacacctg
     1621 caaatttcac gactttaaaa gtgtaatatt ccatatcagt aaccgtcgta tcggtgagtt
     1681 tctgcatggc tttcagcgca agcggcccct ggatggctaa ctgtgaggtt tcgtcggaag
     1741 cattggtaag tttagcacca aactggttat ttttatttac ccaggcccaa tctttgtcga
     1801 tgttggctgc attaaccacc agtaaatatt tttcagcatt gaagcggtaa acgagcagat
     1861 catccacaat gcctccctgg ccattgggat agcaggaata ttgaactttg ccatccacca
     1921 gtttcgatgc atcattggaa gtaacatact gaatgaaatc caatgcttta gggccttcca
     1981 cccaaaactc gcccatgtgc gaaacatcaa aaacgcctaa cgctttgcgg acattttcgt
     2041 gttcaataat taagccctca tactgaatag gcatataaaa accggcaaac tcaaccattt
     2101 tggctccggc agcttcgtga attttacaaa atgctgtagt tttcatcatt atagaatttt
     2161 aaattaatta attggtttct gttttattgt caaatcagat tatcttatca caaagaataa
     2221 caagacaaaa atataaataa taactgatga cagaactttt tgaaccataa taattactac
     2281 ctgcatttag cgctaatttt atttttatct ttggcaccag catttaattt agtaataatg
     2341 cacataacga aattaaacat cccgcgctgg ctcatattgc tgatagacat ttttatctgt
     2401 ggtttttcgg tagtattgac ctatctgctg cgcttcaact tcaacgtacc tgagatcgaa
     2461 tcgcacgatt taccctatgt tcttgccttc actgtcggca tccggttcat tactttcctc
     2521 atcgccaaaa cgtacacagg tatcatccaa tacaccagca caggcgatgc catacgaatt
     2581 gtatttgtta ccgccaccgg ttccatagtg tttgtcatta ccgaccttat cacctatttt
     2641 ttcattaacg gtgttaattt tataccgtat tccatcatca tcattgaatt tatcaccacc
     2701 acgttcctgc ttgccgcatt caggattttg gtcaagataa cttatatgga aatcagcaat
     2761 ccctcgcgcg acaaaaccaa tgtgctgatt tacggtgccg gtgaaagcgg acttatctca
     2821 aaacgctcgc tcgaccgcga tgcaggaacc aaatataaag tgcttgcctt tattgacgat
     2881 gatgcaaaaa agattgggat gaaaatagaa gacatcccca tttatggtcc ggatagatta
     2941 gaaaaactcc ttgaagacaa ccacattgaa cacctgataa tttccataca gaatattaaa
     3001 gccttgaaaa aacaggagat catcgagaaa tgtatggctt acaataccaa agtacttagt
     3061 gtaccgccgg taagcaagtg gataaacgga gagctgagct ttaaacagat aaaaaaaatc
     3121 aatatcgaag acttgctcga gcgtgatgag ataagacttg atgaagaaaa ggtcaggcgc
     3181 gaattattgg ataagtgtat tctggtaaca ggcgctgccg gttcgatagg cagcgaaatt
     3241 gtaaggcaga tagtacggtt ttcgcctaaa aagattatcc tgctcgacca ggccgagtca
     3301 cccatgtatc ttctcgaact ggaactaaac gaaaaatacc ccaaggctaa tattgaggtc
     3361 attatttgcg acatcaacca tcaggtgagg atgcgtaaga ttatggagac tttttcgccg
     3421 caaatcatct accatgccgc ggcttacaag cacgtgccca tgatggagaa caatcctgtg
     3481 gaagccgtaa taaccaacgt gttcggcaca aagaccatag ccgacctggc ggtagaattt
     3541 aaagcggaaa aatttgtgat ggtttccact gataaggctg taaacccaac cagtatcatg
     3601 ggcgctacca aacgcattgc cgaaatctat acccaatcgc tgaacaaaaa aaacgtaacg
     3661 cgctttatca ccacacgctt tggcaatgta cttggctcta acggctcagt gatacccttg
     3721 ttccgcgaac aaattgaaaa gggcggcccg gtaaccatta ccgacccgga ggtaacccgg
     3781 ttttttatga ccataccgga agcgtgccag ttggtgttag aggcaggcac catgggccac
     3841 ggaggagaaa tttttatttt cgacatgggt aaatcagtaa aaattctcga cctcgcaaaa
     3901 aagatgatta aactgtcgcg cctgaccttg ggcaaagaca tacagataaa atatacaggt
     3961 cttcgtccgg gcgaaaagtt atacgaagaa ctgctcaaca acaaggaaaa caccctgcca
     4021 acgtatcatc ctcaaattat gattgccaaa gtggcagaaa gtgattttga gttgatttcg
     4081 aaacaagtaa tggaactgga gagtctgctc gacagccacg acaacattca gatcgttaaa
     4141 aaaatgaaag agattgttgc tgagtttaag agccagaatt cggtgtatgc aaaattggat
     4201 tagtgacagg ttaaaaattc ataagtaagt gtcggggtga ctgctcttgg gctatgctgc
     4261 tgcaagtcac ccagacactt tcacaaaaaa aatcaatcct tcagcacctt attcaccagg
     4321 tctgcggctt cctgcaactt tatagctgaa aatactttca ggccggaatt atcaataatt
     4381 tctttggctt cttcggcgtt ggtaccctgc aagcggacta ttaccgggac ttttatttct
     4441 cctaaacttt tgtaggcatc cactatgccg ttggctaccc tgtcgcaacg cacaatacct
     4501 ccgaaaatat ttaccaaaat ggctttcaca ttttcgtcgc tgagaataat gcgaaatgcc
     4561 tgttccacgc gtttggcatt ggcggtgccg cccacatcaa gaaaattggc cggcgagccg
     4621 ccgctgagtt ttatgatgtc catggtagcc atggccagtc cggcgccatt taccatacac
     4681 cccacgttac catccagttt aatgaaattc aggccatatt gcatggcttc cacttcaacc
     4741 gggttttctt ctgccaaatc gcgcatattt gcaataatac aatggcggaa gagagcattg
     4801 ttgtcggcca ccatcttggc gtcggcggca aaaatcttat cgtctgaagt ttttattacc
     4861 gggttaatct ccagcagagt caggtcgcag ccggtataag ctttgtaaag gttttttaca
     4921 aagggaagca tttttttata agcttcgccc tccagcccca ggttaaaagc aatacggcgg
     4981 cattgaaagt ctctgatgcc tacacccggg tggatttctt ccttaaaaat ctgttccggt
     5041 gtttcttcgg ccaccttttc aatatccata cctccgcgcg gcgaatacat gatgacattc
     5101 ttccctgtat gccggtccag cagaatacct atataaaact ctcttattgg ctgggggccg
     5161 ggataataga tattctgttc cacaagaacc ttatgcactt ttttgccttc ggctttggtt
     5221 tgcggggtta tcagctgcat cccgataatg tgccgggcat gctcaaagac ttcgttggag
     5281 ccggaagcga ttttcactcc cccgcccttt cccctgcctc cagcatgtat ctgtgctttg
     5341 accgcaaatt tttccgatcc tgtgccggcg ccaataatca gggccgcatt catggcttcg
     5401 tcaggagtgt tagccacgat gccttcaggg acactgaccc cgaaagtttt caaaagttct
     5461 ttggcttgat attcatgaag attcataaaa atattttact aattgataat caaagataat
     5521 gaaaaatgcc ttcgaaaaaa agtgtgtaca aaaaaaccta attaaaaacg tcaagcatct
     5581 atttgtttag attaaataat tattttcgca agaatgaaat cagactataa aatattttgc
     5641 ctgtttttat ttattctgat gttttgtctg cctgcagcca tggcacagaa aaaaaccgcc
     5701 tatgccacaa gaataacggc acctcccgac attgacggtg tccttaccga tgcatgctgg
     5761 cgcctgtgcg attcggctga cagtttcagg caatatgttc ccgtttacga cgtaaacccc
     5821 acacaacgta ccgtggtacg cattgcgtat aacgacgagg ccatctttgt cggcgccatg
     5881 atgtacgaca atgcgccgga cagcattctg acagaattcg gggttcgcga cgacgaactt
     5941 aatgctgacc gatttgccat acaatttgac acctatcaca tgcagaccga tgcatacacc
     6001 tttgccgtct gggcttcggg cacacaggcc gactggcggc acaccgacga aacctataat
     6061 gctgtatggc aaagcgctgt taagattaat gacaagggct gggcctgcga aatgaaaatc
     6121 agctactcgg ccctgcgttt tcccaagaca gcagtgcagg aatgggggct tcagattttc
     6181 cgggggatac ggcgccaccg cgaaacagac cagtgggcgc tcgaagtaaa aagcaatgcc
     6241 aatttcctga ctgactgggg aaaactggaa ggtatcaggg atgtaaaacc cgcgcttcga
     6301 ttatcactga cgccttttgt ttctctgtat ggcgaccatt atccatacaa tgaaaaaggg
     6361 aaaagtaatt tctcgtattc tttcagtggc gggttagacc tgaaatacgg tataaatgaa
     6421 agcttcacgc tcgacatcac cctgctgccc gatttcagcc aggttcagtc ggattatcct
     6481 gtcaaaaata tttcggcaca ggaaaccgtg tacgaagagt accggccgtt ttttacggaa
     6541 gccattg
//
LOCUS       DAOSFP010000096         6501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10378_length_6501_cov_3.918864, whole genome shotgun sequence.
ACCESSION   DAOSFP010000096 DAOSFP010000000
VERSION     DAOSFP010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10378_length_6501_cov_3.918864"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="PLI16_04095"
     CDS             <1..1479
                     /locus_tag="PLI16_04095"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOH83769.1"
                     /translation="PVVLFSVGCAYGIHVYNRFRMVVRTPENKLRQSAEALSRIGTAV
                     LLSGITTVMGFLSFIFGAYLNIIFEFGVFAALGVLFILILSITFIPALLSYFPVEKIH
                     KINSRYRIYNPLDTFLTKLADVIISHPKKILVVSGIFLLVMAAGIPFLKNKIDILNYF
                     RPHTDIRVSASIMNNEFGGSLPLMVRVRGDIQDPVTLIKMKKVKDFLEKQPEIRNASY
                     VGDFLEEMNDCMGEGKCLPDTREKVANLLFLIEGDEMLKQMINADKTEAIIQAYMRNV
                     ETKRYKELFSSVNTLLSELNSPEVNLAQTGMPGVYSNFDDSLMKNLLQSLILALALIF
                     ICMVFLLRSVRSSFVGMIPLLFTIIIIFGFMGISGIALDICTILIASITVGAGIDYAI
                     HFITEYKHHIIQGKSVEDAIAFAVRISGKPICINVITVMLGFLVLIFANLIPLLNFGI
                     LIAITMFFSGLGAITILPAVISLFNLKLVKDSDIKITRIEKF"
     gene            1501..2232
                     /locus_tag="PLI16_04100"
     CDS             1501..2232
                     /locus_tag="PLI16_04100"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HOH83770.1"
                     /translation="MFLIPVLSAALLLLHGKVSAQDAAGILQKVDQILNAPKDQKIMT
                     TITLYDKQSRQSLRTMSMVQKGSDKRLVKILSPADQKGIGFLSLPNDNLTVYLPAYGK
                     TRKISSSVKNSKFAGTDFTYEDMEAKKYSQKWTPKLINTTGEHFVLQLNPRDGVISDY
                     SKLIMYVRKSDNYPIKVEHFDKSGKLYKVLTDNNVVKTDGYQIAKELVMEDKKSGTKT
                     KMTVTSVTFDNGLKDDYFSERELIK"
     gene            2251..3810
                     /locus_tag="PLI16_04105"
     CDS             2251..3810
                     /locus_tag="PLI16_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83771.1"
                     /translation="MKKKFFFGFCILLSIGLKAIAQQDTSFIVQPDTSGNLFADTSAV
                     FPEAGVMAPDDGLLPEEPKLSINGYIQADDRLKFDGGKLYRQEYRLDVKLDYKPFDRG
                     KLFAEVWLRTYKHPEIYNVGDLSDKRKILNFDADVREAYFDIYGLFTKNLDIRIGRQR
                     IAWGTADRLNPTDNLNPYDMEDIWDFGRHLGSNAIKLIYYAGTFTFTGVAIPWFTPSV
                     LPGSDWTQAFMPEVKIPSMITDTSFMPGLKIPIYLTPRSISDTLVMPQKDIRHYPSFG
                     LKAKKTLRNFDISLSYVYGRDALPLPTELYTNITIDTFAVFPEVRADATVDIRSKVEF
                     PVMQVVGLDFAGSIKDVGIWGEAACFLPGKNYMKRYLHYDFPDYGISKDSVLSDSLVL
                     DSKPYVKFVVGLDYTFKHGFYLNFQYLHGFVHERGHKELNDYYMLAFEWTSGNGRIKI
                     SILNGGIQINQYKGLKNNLAWIYMPEISYKPVDNAELILGAHLLDGSPTTSFGKVTKN
                     DDIYLKLKYSF"
     gene            3935..5701
                     /locus_tag="PLI16_04110"
     CDS             3935..5701
                     /locus_tag="PLI16_04110"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOH83772.1"
                     /translation="MSKANENKVYDLGVSSHAGQVMQENQDCYTDFECINGHVFIVCD
                     GMGVHEAGAVASQLAVESIRAYLENHYFDMPEDALKAAIEFANSVVYKRSKLKPAYHG
                     MGTTIVMAMLRNNKFHYAHVGDSRVYIFSDGKLHRLTRDHSQVQDMVDNGLLSDGEAR
                     SHPDRKKITRALGSGLNIEIEFCTAPALPALNDFILMCTDGLFFMLDDIEISQVLSNT
                     DPVQEKAEKLIELTNAKGGPDNITALLIYFVGIANKKAVFSPVFYVPKEMKPLIKKEA
                     PQENFDTATSEITPKEPAEQEEYTSGKPLDTVFMQNEIIDEEPEEEEPVGEAPVEEEY
                     LDEETVDEQPLQETTPFETTGITEKTEDDFVKKSIPKEEQPAYHEETKTQEPAKEIYP
                     EEASAERTERTENVTITTFLNRFGFYRKLKEKPDLMRKIRIGAIILGVLFVAYVFWDI
                     FIKQSAPTQVTEEKKKPAAVTEEEPKDSVPATSQTVAQPDTIWISYSVKKGEFLGTIA
                     SKFGVTVDFIKKKNKLPNDNIREKQKLEIPTRANHTVKAGESFASIAKKYNLQAKSVM
                     RANDIKDEKSIKTDKSLIIPFK"
     gene            5709..>6501
                     /locus_tag="PLI16_04115"
     CDS             5709..>6501
                     /locus_tag="PLI16_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83773.1"
                     /translation="MAAATPKKHQKIPAGRKQDTAKPTGRVSIAEAAMPDKYIKWIVF
                     AFALLLYANTLDLKYALDDSLMITDNNFTKKGASGIKDILTNDAFVGFLGKNNLLPGG
                     RYRPLPQVMFAIEKEFFGFNPFVGHLINILLYAFTCMLLFIILNRLFINYKTTNWYLG
                     IPFTATMLFTAHPLHTEAVANIKGRDEILCLFFCLLVVLFTLQYLEKKKIYLLIANLF
                     LFLLALLSKENALTFAAIIPLILFVFFQPRLKDYLIVSAPLLGGIA"
BASE COUNT         1925 a         1490 c         1413 g         1673 t
ORIGIN      
        1 ccagtggtat tgttttcggt gggttgcgca tacgggatcc atgtgtacaa tcgtttccgg
       61 atggtggtgc gcactcctga aaataaactc cggcagtcgg ccgaagcact gagcaggata
      121 ggaaccgccg tgctgctgtc aggcataacc actgtgatgg gctttttatc ctttattttt
      181 ggggcctacc tgaatattat ttttgagttt ggtgtttttg ccgccctggg cgtgttgttt
      241 atcctcattc tttccattac atttattcct gcgcttttgt cgtattttcc tgtcgaaaaa
      301 atccataaaa taaattcacg ctacagaatc tataatccgc tcgacacatt tctgaccaaa
      361 cttgccgatg tcataatctc tcaccccaaa aaaatactgg tggtgtccgg cattttcctg
      421 ttggtgatgg cagcaggtat tcctttcctg aaaaacaaaa ttgatatact gaactatttc
      481 aggccgcata ccgacatccg tgtttctgcc tccataatga ataacgagtt tgggggctca
      541 ctgcccttaa tggttcgtgt gcgcggcgac atacaggatc ctgttacgct gataaaaatg
      601 aaaaaagtaa aggattttct cgaaaaacag cccgagatcc ggaacgcttc gtatgtaggt
      661 gattttcttg aagaaatgaa cgactgtatg ggcgaaggca aatgcctgcc cgacacccgc
      721 gaaaaagttg ccaacctgtt gtttcttatc gaaggcgacg agatgctgaa acaaatgatt
      781 aatgcggata aaaccgaggc catcatacag gcatatatgc gtaatgtgga aacaaaaaga
      841 tataaagagt tgttctcatc ggtcaacacg ttgctttcgg aattaaattc accggaagta
      901 aatctcgccc aaaccggcat gccaggtgtc tattcaaatt ttgatgacag cctgatgaaa
      961 aacctcctgc aaagccttat cctggcactg gctcttattt tcatctgcat ggtgtttttg
     1021 ctgcgttcag tgcgcagttc ttttgtaggg atgattccgc tattatttac tatcatcatt
     1081 attttcggat tcatgggtat ttcgggcatt gcgcttgaca tttgcaccat actcatagcc
     1141 agcattacgg ttggtgcggg catcgattat gccattcatt ttataacgga atacaaacac
     1201 catatcatcc agggaaaaag tgttgaagat gccatagcct ttgcggtgag aatctccggc
     1261 aaacctatat gtatcaacgt gataacggtg atgctgggat ttctcgtact gattttcgcc
     1321 aacctgattc ccctgctgaa tttcggaata ctgattgcca tcaccatgtt cttcagcggc
     1381 ctgggcgcca taaccattct gcccgcggta atcagtttgt ttaatttgaa actggtgaaa
     1441 gatagcgata taaagattac acgaattgaa aaattttaaa cttttacgac catgaaaaaa
     1501 atgtttttaa ttcccgtcct ttcagcagca ttactgctgt tgcacgggaa agtatccgca
     1561 caggatgctg ccggtattct gcaaaaagtt gaccaaatcc tcaatgcgcc aaaagaccag
     1621 aagataatga ctaccatcac cctctacgac aagcaatcgc gacagagcct gcgaacgatg
     1681 agcatggtac agaaaggcag cgataaaaga ctggtaaaga tactctcgcc tgccgaccag
     1741 aaaggcatag gatttttgtc gttacccaac gacaacctca cggtgtatct gccggcctat
     1801 ggcaaaaccc gcaaaatttc ctcctcggtg aaaaactcaa aatttgccgg taccgatttt
     1861 acctatgagg atatggaagc aaaaaaatac tcacaaaaat ggacacccaa gcttatcaac
     1921 actaccggcg aacactttgt ccttcagctt aatccgagag acggcgttat cagcgactac
     1981 tcaaaactca tcatgtatgt aagaaaatcg gacaattacc ccataaaggt ggaacatttc
     2041 gacaaaagcg gtaaactcta caaggtgcta accgataata atgtagtgaa aaccgatgga
     2101 taccagatcg ccaaagaact ggtaatggaa gataaaaaat caggcaccaa aaccaaaatg
     2161 acggttactt cggtgacttt cgacaacggg ttgaaagatg attatttttc agaacgcgaa
     2221 ctgataaaat aaaacaccgg attacccatt atgaaaaaaa agtttttttt cggtttttgt
     2281 atattgctgt ctataggtct taaggccatt gcccaacagg acacatcgtt tatcgtgcag
     2341 cctgacacgt caggaaatct gtttgctgac acctcggcag tgtttcccga agccggtgtt
     2401 atggcacctg atgacggact attacccgaa gagcccaaac tgtctattaa cggatacata
     2461 caggctgatg acaggctgaa attcgatgga ggcaagctct accgacagga ataccgcctg
     2521 gatgtaaaat tagattacaa accttttgac cgcggaaaat tatttgccga agtgtggctg
     2581 cggacgtata agcaccctga aatttataac gttggcgact tatcggataa aagaaagatt
     2641 ttaaattttg atgccgatgt acgtgaggcc tattttgaca tttacggatt gtttacaaaa
     2701 aatctcgata tcaggatagg caggcagcgc attgcatggg gcacagccga ccggctgaat
     2761 cccacggaca acctgaaccc ctatgacatg gaagacatct gggactttgg ccggcacctg
     2821 ggttccaacg caataaaact tatttattat gcgggaacat ttacctttac gggggtggca
     2881 atcccatggt ttacaccttc ggtattgccc ggcagcgact ggacacaagc ctttatgccc
     2941 gaagtaaaga ttccttccat gattaccgac acttcgttta tgcccggcct caaaatcccc
     3001 atctatctaa caccacgtag catctcggat acgcttgtaa tgccgcaaaa agatatcagg
     3061 cattatccgt ctttcgggct gaaagcaaag aaaacgctga gaaatttcga catctcgctg
     3121 agctatgtgt atggccgcga tgcgctgccc ttgcccaccg aactatacac caatattacc
     3181 atcgacactt tcgccgtttt tcctgaagta cgcgccgacg ctacggtgga tatcaggtcg
     3241 aaagtagagt ttcccgtgat gcaggttgtg ggacttgact ttgccgggtc catcaaagat
     3301 gttggtatct ggggcgaggc agcctgtttc ctgccgggaa aaaactacat gaaacgttac
     3361 ctgcattacg attttccgga ttatgggata agcaaagatt cggtgcttag tgattctctg
     3421 gtactcgaca gcaagccgta cgtgaaattt gttgtagggc tggattatac atttaaacac
     3481 gggttttacc tcaattttca atacctgcat ggttttgttc acgaacgcgg gcataaagaa
     3541 ctaaacgatt attatatgct tgcctttgaa tggacctcgg gtaatggccg gataaaaatc
     3601 agcatattaa atggcggcat acaaataaac caatacaaag gcctgaaaaa caaccttgcc
     3661 tggatatata tgcctgaaat ttcgtacaaa cccgtggata atgccgaact catattagga
     3721 gctcacctgc tggacggcag ccccaccaca agctttggca aggttaccaa aaacgatgac
     3781 atctacctga aactgaaata cagtttttga tatttttctg ttgttggccc tgctgtttta
     3841 ctaacaatca tgttgttgaa attcagttta tacttctgct gctaaccatt ttttctttag
     3901 tatatttgtc cgcgaacttt taagcaaaaa aagtatgtca aaggcaaatg aaaataaggt
     3961 ttatgatttg ggtgtttcca gccatgccgg ccaggtaatg caggaaaacc aggattgtta
     4021 tacagatttt gaatgtatca atggtcatgt ttttatagtg tgcgacggca tgggtgttca
     4081 tgaagccggt gcggtagctt cgcagcttgc cgtggaaagt atccgcgcat acctggaaaa
     4141 ccactatttc gatatgccgg aagacgcctt aaaagccgcc atcgaatttg ccaattccgt
     4201 ggtgtataaa cgatcgaaac tcaaacccgc atatcatggt atggggacca ctatcgtgat
     4261 ggccatgctg cgcaacaaca aatttcatta tgcacatgtg ggcgacagcc gggtatatat
     4321 cttttcggac ggcaaactgc accggctcac acgcgaccac tcgcaggtgc aggacatggt
     4381 cgataacgga ttgctgtccg acggggaagc ccgctcgcat cccgatcgaa aaaaaattac
     4441 ccgtgcgctg ggttccggcc tcaacattga aatcgaattt tgcacagccc ctgccctgcc
     4501 ggcgctgaat gattttattc tgatgtgtac tgatggactg ttttttatgc ttgatgacat
     4561 tgaaatatca caagtgctca gcaacacgga cccggttcag gaaaaagcag aaaaacttat
     4621 tgagctcacc aacgccaaag gaggaccaga taatatcacg gctctgctca tttattttgt
     4681 tggcatcgct aacaaaaaag cagtgttttc cccggtattt tatgtaccaa aggaaatgaa
     4741 accgctaatc aaaaaagaag cgccacaaga aaatttcgac accgccacaa gcgaaataac
     4801 ccctaaagag cctgcagaac aggaagaata tacttccggc aaacctttgg ataccgtgtt
     4861 catgcaaaac gaaataattg acgaagaacc ggaagaagaa gagccggttg gtgaagcacc
     4921 cgttgaagaa gaatatcttg atgaagaaac ggttgatgag cagcccctgc aggaaacgac
     4981 accttttgaa acaacgggca tcactgaaaa aacagaagat gattttgtga agaaaagcat
     5041 tccgaaagaa gaacagcctg cttatcatga agaaacaaaa acccaggaac ctgccaaaga
     5101 aatctatcct gaagaagcat cggctgaacg aactgagcgc acagaaaatg ttactatcac
     5161 cacgtttctg aaccggttcg ggttttacag aaagctgaaa gaaaaaccgg atttgatgcg
     5221 caaaataagg ataggagcca ttattctcgg ggtcttattt gtggcctatg ttttttggga
     5281 tatctttatc aaacaaagcg cacccacgca ggttaccgaa gaaaaaaaga aacccgcggc
     5341 agttacagaa gaagagccca aagacagcgt cccggctaca agccagacag tagcccaacc
     5401 cgacaccatc tggataagct attcggtgaa aaaaggagaa ttccttggca ccatcgcttc
     5461 caaattcggt gttacggtag attttatcaa aaagaaaaac aagcttccta acgacaatat
     5521 ccgcgaaaaa caaaagctgg agatacctac cagggccaac cacaccgtaa aagccggaga
     5581 atcatttgca agcattgcca aaaaatataa ccttcaggca aaatcagtga tgagagccaa
     5641 cgatatcaaa gacgaaaaaa gcatcaaaac ggataagagc cttattatcc cttttaaata
     5701 aaagcccgat ggctgctgcc actccgaaaa aacaccagaa aattcctgcc ggcaggaaac
     5761 aggatacagc gaagccaaca ggcagggtta gcattgctga agccgccatg cctgacaaat
     5821 atatcaaatg gatagtcttt gctttcgccc tgttgcttta tgccaacacg ctcgacctga
     5881 agtatgccct ggacgactcc ctgatgataa ccgataacaa ctttaccaaa aaaggagcct
     5941 ccggcataaa agatattctt accaatgacg ctttcgtggg cttcctgggc aaaaacaacc
     6001 tattgcccgg cgggcgctac cggccgctgc cgcaggtgat gttcgccatc gaaaaagagt
     6061 tcttcgggtt caaccctttt gtagggcatc tgataaatat cctgctgtat gctttcacct
     6121 gcatgctgct gtttatcatc ctcaacagac tttttattaa ttataagacc acaaactggt
     6181 accttggcat tcctttcaca gcaactatgc tgttcacggc gcaccccttg catactgagg
     6241 cggtagccaa cataaaaggc cgcgacgaaa tactttgttt atttttctgt ttactggtgg
     6301 tgttgttcac attgcaatac ctggagaaaa agaaaatata cctgttgata gccaacctgt
     6361 tcctgtttct gctggcactg ttatccaaag aaaatgcgct cacctttgca gccattatac
     6421 ccttgattct ttttgtattt tttcagccga ggctcaaaga ttaccttata gtttcggccc
     6481 ccctgctcgg tggcattgca g
//
LOCUS       DAOSFP010000097         6479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10438_length_6479_cov_3.108811, whole genome shotgun sequence.
ACCESSION   DAOSFP010000097 DAOSFP010000000
VERSION     DAOSFP010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10438_length_6479_cov_3.108811"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3073
                     /locus_tag="PLI16_04120"
     CDS             <1..3073
                     /locus_tag="PLI16_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HOH83774.1"
                     /translation="KDNQSINPETIFTVNKSEFGLSQADQMVIKRTEADQLGFTHHRY
                     QQYYKGISVEGGEFIIHSAAGKAITGNGKIITGLNLATTPALSPQQAVDKALAFTHAE
                     KYMWEDPGSEAMLKKARKDPAATYQPKAKLVVIDKKYGKDASKYILAYKVEVYAAKPL
                     SYKNIYVNANSGAVYHTMDRLHLTDVPALAHTKYSGTQNITVDSTGPGSYRLHETGRS
                     GVETYNMNSGTSYSAAVDFTHPDTDWTLTNAQQDEVGLDAHFGAEATYDFYWNSFGRD
                     SYDDNGAPLISYVHYDVDYDYAFWNGTVMTYGDGDGSTTGPFTALDVCAHELTHGVTE
                     YTANLIYQDEPGALNEAFSDIFSAAVEFYATPSQADWFVGEDFDLSGGNGFRNMSDPN
                     EDDQPDTYLGTNWYSGTMDNGGVHYNNSVGNYWFYLLSVGGSGTNDLGNYFSVDSIGI
                     EKAARIAYRTLSVYLTENSQYADARMASIQAATDLFGHCSNEMIQTVNAWYAVGIGMA
                     VADNDVYISEVVNPVTACGMTYEPVSVNMIYNGCSQPLNANDTIKFFYQVDGGTVVGD
                     TMILANTINGGDTINFTFSVPADVQALGMHSITCWLNYANDTVNYNDTLASYSFENRL
                     YQNSDVGVTGIVSPESACNLSASEDVTIKLGSFACDFLPAGAAIPVGYSINNGPYVYD
                     TITILSDMYPDSVILHTFSIPADLSATGISYTIKAKTFFADDSLSTNDEYAGYVVINP
                     ASLSDTIVTFEQANASDMYLVTLGDYANASIANAAHHTGTKGFRMTGGNALSYYTMLE
                     FPDGFNTWTINDFLSAKITFCVDATAWSNAYLRFSLKQTFGQAGYEYILGQGYDYSIA
                     SNFRVLVNDVTQIGGTYNPTTANSDPFVSHFLDLSTYAGSKFTVTFETRNICKDTLIF
                     TMDNAYVDNVKFMNAIDDIGIEAEDISNYLDIFPNPVSEMLNVNLFSNEQQAVNIEVF
                     DIQGKIMESKTENAIKGQNFFKLDMNGKPAGMYFIRISTNKGIFNDKIMKK"
     gene            complement(3076..3447)
                     /locus_tag="PLI16_04125"
     CDS             complement(3076..3447)
                     /locus_tag="PLI16_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83775.1"
                     /translation="MINSCKTSEVQRSFMLGKGGGFTGKYEIFLVKSNGEVFKVINEQ
                     PAGEVAVKIDKKQIAEVFSEFDKLNIAGDSFSYPGNMTYFIRYTDNNKTYEIKWGSPN
                     VAPPQNYVAFFEKTWSLIRKK"
     gene            complement(3635..5458)
                     /gene="argS"
                     /locus_tag="PLI16_04130"
     CDS             complement(3635..5458)
                     /gene="argS"
                     /locus_tag="PLI16_04130"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOH83776.1"
                     /translation="MILLQRMEQQFRLEPLIINTVSQAFKQLYGQEPETRLISIQKTN
                     LQFRNEADFTIMVFPLVRLSKKSPEMTAKELGDYLQEHLDFVENFNVVKGFLNIKISS
                     KCWLDFLRKHIGYMHYGMQFPDSAQPPVIVEYSSPNTNKPLHLGHIRNNLLGDSISRI
                     LKANGKNVVQANLVNDRGIHICKSMLAWQKWGRGETPESSDMKGDKLVGKYYVEFDKH
                     YKEEVKQLVATGMEEEEATKAAPLMQEAQEMLRQWEQGSQEVRTLWQMMNSWVYQGFE
                     QTYARLGISFDKVYYESNTYLLGKEVVAEGLNKKVFLKKDDGSVWVDLTAEGLDEKLL
                     LRADGTSVYMTQDLGTACMRYEEFHPEKMIYVVGNEQNYHFDVLKKVLARAGYPWAEN
                     IRHFSYGMVELPEGKMKSREGTVVDADDLIDEVTLSARQITSELGKIDNFDSEEAEQL
                     FRMIGLGGLKYFILKVDPVKNMLFNPAESIDFDGNTGPFIQYTHARIKSLLRKSGKDM
                     ASLTFDDFNGLLDEERELLFLCYDFRRILSLAADELSPAQIANYVYELAQQYNKFYQR
                     IPVLKDEDVNRVNFRLALSFFTANIIKNAMDLLGISVPEKM"
     gene            5721..>6479
                     /locus_tag="PLI16_04135"
     CDS             5721..>6479
                     /locus_tag="PLI16_04135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH83777.1"
                     /translation="MNQQDRKKKTAYTGKAKKTAIKQEKIANKRWSSYVLSALLIITA
                     AIFFQAIKFDFLTTWDDNFYVTENPDIKALTWSNIQLLFTKSYMNMYQPLTMVLYAFE
                     YQLGGGNAAIFHFVNVLFHVLNTWLVYVFIKKITKGNQVVALFTAAFFGVHPMHVESV
                     AWISETKDVLYSFFFLLSLITYHNYLKDNKIKHLIWACMLFLLSSMSKSAAVILPLVM
                     LLMDYYTGRKFSMKMLVEKIPFFIISLVFGLISLK"
BASE COUNT         1807 a         1443 c         1358 g         1871 t
ORIGIN      
        1 caaagataat caatctatta atcctgaaac gatttttaca gtcaacaaaa gcgaatttgg
       61 cctgagtcag gccgaccaga tggtgataaa acgcacggaa gcggaccaac tgggtttcac
      121 gcatcaccgg tatcagcaat attataaagg catttcggta gaaggtggtg agtttattat
      181 tcacagtgct gccggcaagg ctataacggg taacggaaaa atcattaccg gcctgaacct
      241 ggccactacc cctgccctgt cgccgcaaca ggctgttgac aaagccctgg catttaccca
      301 tgctgaaaaa tatatgtggg aagatcctgg cagcgaagct atgctgaaaa aagccagaaa
      361 agaccctgct gccacttacc agccgaaggc aaaactggtt gttatcgata aaaaatatgg
      421 taaagatgct tcaaaatata tcttagctta taaagtggag gtttatgcgg ccaaacccct
      481 gtcgtataaa aatatttatg tgaatgccaa ttcgggggct gtttaccata ccatggaccg
      541 tctgcacctt accgatgtgc cagcattggc gcacacaaaa tacagcggca cacaaaatat
      601 taccgtggac tctaccggac cggggtctta ccgtttgcac gaaacaggac gcagcggtgt
      661 ggaaacttat aatatgaact cgggtacctc gtacagtgct gccgtggatt tcacccaccc
      721 cgacaccgat tggacactca ccaatgcaca gcaggacgaa gttgggctgg atgctcattt
      781 tggcgctgaa gcgacctatg atttttattg gaacagcttc ggtagagaca gttacgacga
      841 caacggtgca cctttaatca gctatgtcca ttatgatgtt gattacgact atgctttctg
      901 gaatggcacc gtgatgacat acggcgatgg cgacggctcc acaacaggcc cttttaccgc
      961 cctggatgtg tgtgcacatg agctcactca cggcgttact gaatacaccg ccaatctgat
     1021 ctatcaggat gagccgggtg ccctgaatga ggccttcagc gatattttca gcgctgcagt
     1081 tgaattttac gccactcctt ctcaggccga ctggtttgta ggggaagact ttgacctgag
     1141 cggtggcaac ggattccgca atatgtccga ccccaacgaa gacgatcagc ccgacactta
     1201 cctgggcacc aactggtatt ccggcaccat ggataatggc ggtgtacatt acaataacag
     1261 cgtgggcaac tattggtttt acctgttgtc ggttggcggc tcagggacca acgatcttgg
     1321 aaattatttt tcggtggaca gcatcggcat tgaaaaagcc gcacgtattg cctaccgcac
     1381 cctttcagta tatcttaccg aaaattcgca gtatgctgat gcccgcatgg cttctataca
     1441 ggctgccacc gacctgttcg gacattgctc caatgaaatg atacaaactg taaatgcctg
     1501 gtatgccgtc ggtataggta tggctgtggc cgacaatgat gtttatatca gcgaagtagt
     1561 caatcctgtt accgcctgtg gtatgaccta tgagcctgtt tctgttaaca tgatatacaa
     1621 tggctgcagc cagccactca atgcaaacga caccattaaa ttcttttatc aggttgatgg
     1681 tggcactgtg gtgggcgata ccatgattct ggccaacact attaacggcg gcgacaccat
     1741 taactttaca ttttcggttc ctgccgatgt tcaggcactt gggatgcaca gcatcacttg
     1801 ttggttaaac tatgccaacg atacagtaaa ttataatgac accctagcct cttatagctt
     1861 tgaaaacagg ctttaccaaa acagcgacgt gggagtaaca ggaatagttt cgcctgaatc
     1921 agcgtgcaac ctgagtgctt cggaagatgt taccatcaaa cttggttcct ttgcctgtga
     1981 tttcctcccg gccggtgccg ctatccctgt ggggtacagc attaataacg ggccctatgt
     2041 gtacgacacc attaccatac tttcggatat gtatcccgat tcggttattc ttcatacttt
     2101 ctctattcct gcagaccttt ccgcaacggg tatcagttat accataaaag caaaaacatt
     2161 tttcgccgac gacagtttaa gcaccaacga tgaatatgcc ggctatgttg tcataaaccc
     2221 cgcatcgctt tccgacacta ttgtaacctt tgaacaagcc aacgcttcag acatgtacct
     2281 ggtaactcta ggggattatg ccaatgcctc tattgccaat gccgcgcatc atacggggac
     2341 taaaggattc cgcatgacag ggggaaatgc attaagctat tacaccatgc tggaatttcc
     2401 ggatggattt aacacctgga ctatcaacga ttttctgtcg gccaaaatta cattctgcgt
     2461 ggatgctaca gcctggagca acgcctacct gcgattttcc ctgaaacaga ccttcggcca
     2521 agccggatat gaatacattc ttggacaagg ctatgattat tctattgcca gcaacttcag
     2581 ggtattggtc aatgatgtga cgcagatcgg cggaacatac aaccccacca cagccaacag
     2641 tgatcccttt gtatcgcatt ttctggattt gagtacctat gccggctcaa aatttacggt
     2701 gactttcgaa acccggaata tttgcaaaga cacgctgatt ttcaccatgg acaacgctta
     2761 cgtagataat gtaaaattta tgaacgccat tgatgatatc ggtattgaag ccgaggatat
     2821 cagcaactac ctggatatat ttccgaaccc ggtcagtgaa atgctcaatg taaatctgtt
     2881 ttcgaacgaa caacaagctg taaatatcga agtctttgat attcagggaa aaattatgga
     2941 aagtaaaacg gaaaatgcta tcaagggaca gaactttttt aaactggata tgaacggtaa
     3001 accagccggc atgtatttca tcagaatatc aaccaacaaa gggattttca atgataaaat
     3061 catgaaaaaa taggtttatt tttttcggat aaggctccag gtcttttcga aaaaagctac
     3121 ataattttgg ggaggcgcca cattggggct tccccatttt atttcatagg ttttgttatt
     3181 atcagtatag cgaataaagt aagtcatatt gcccggataa gaaaaactgt ctccggcgat
     3241 gttcaactta tcaaactcac tgaacacctc agcaatttgc tttttatcaa tttttactgc
     3301 cacttcgcca gccggctgct cattgattac tttgaaaact tcgccattgg actttaccag
     3361 aaatatttcg tactttcccg taaacccacc tccttttccg agcataaaag aacgttgaac
     3421 ttcacttgtc ttgcaagaat ttatcattat tataaatatg attataaata atttaatata
     3481 tttcatttgc agttttagtt taaattaaat cggtttggat tttactgttt tttgcagata
     3541 tgagagcctg ttaaaatctt ttcaaaggac taaatggagt tctctatttt aatagccaaa
     3601 tgggtttacg tcctgttggt ttattatctt attattacat tttttcaggg acagaaattc
     3661 ccagcaaatc catagcgttt ttaatgatat tggctgtgaa aaacgacaag gcaaggcgga
     3721 aattaaccct gttgacgtcc tcatctttta aaacagggat acgttggtaa aacttattgt
     3781 attgctgtgc caactcatac acgtaattgg ctatctgggc gggacttagt tcgtcggcag
     3841 ccagcgacaa aatacgtctg aaatcataac acaagaacag caactcgcgt tcttcgtcga
     3901 gcaagccatt aaaatcatca aaagtgagtg aggccatgtc tttacccgac ttacgcaaca
     3961 aggactttat gcgggcgtgg gtgtactgga taaatggacc ggtgttccca tcaaaatcaa
     4021 tggattcggc aggattaaac aacatatttt tcacgggatc cactttcagg ataaagtatt
     4081 taagaccgcc caggcctatc atccggaaaa gctgttcggc ttcttcgcta tcaaagttgt
     4141 caattttacc gagttcgctg gtaatttgcc gtgccgacag ggttacttca tcaataaggt
     4201 catcggcatc tactacggta ccttcgcgcg atttcatttt gccttcgggc aattccacca
     4261 tgccgtaaga aaaatgtctg atgttttcgg cccagggata gccggcgcgg gctaacactt
     4321 ttttgagcac atcaaaatga taattttgtt cattacccac cacgtatatc attttttcgg
     4381 ggtgaaattc ttcgtagcgc atacatgcag tgcccagatc ctgagtcata tatacagagg
     4441 tgccgtcggc tctcagcagc agtttctcgt ccaatccttc cgcggtaagg tcaacccaca
     4501 ccgagccatc gtcttttttc aggaacacct ttttattcaa gccttctgcc actacctctt
     4561 tacctaaaag gtaggtattt gattcataat acactttatc aaaagatatc ccgaggcgtg
     4621 cgtaagtttg ttcgaaaccc tgatacaccc atgagttcat catctgccaa agcgtcctga
     4681 cttcctgtga gccctgctcc cattggcgca acatctcctg tgcctcctgc atcagcggtg
     4741 ccgctttggt tgcttcttcc tcttccattc ctgtggcaac caattgcttc acctcttcct
     4801 tataatgttt gtcaaactca acataatact tgcctaccag cttatcgccc ttcatatcag
     4861 acgattcagg agtttcgccc ctgccccatt tttgccaggc cagcatcgat ttacagatat
     4921 gtatgccgcg gtcattcacc aggttggctt gtaccacatt tttcccgttg gctttcaaaa
     4981 tacgtgaaat ggaatcgccc agcaggttgt tacggatatg gcccagatgt aagggtttgt
     5041 tggtatttgg cgaagaatat tcaacaatta caggtggttg ggcactgtca ggaaactgca
     5101 tgccatagtg catatatcct atatgctttc ttaaaaaatc aagccagcat ttcgacgata
     5161 ttttaatatt gagaaatccc tttactacat taaaattctc aacaaagtcg aggtgttcct
     5221 gaagataatc gcccaactcc tttgcagtca tttcgggaga ctttttggag aggcgtacca
     5281 atggaaacac catgatggta aaatcagctt cgtttcggaa ttgtaaatta gtcttctgaa
     5341 tactgataag ccgggtttcg ggttcctgcc catataattg tttaaaggct tggctgaccg
     5401 tgtttatgat aagtggttcc aacctgaatt gctgttccat acgctgtaat aatatcatgc
     5461 aaagatataa attgttcggt ttttcaggca ctttgttggt aaaaatgcct gttttattgt
     5521 gacagctcct gtatcataaa acctctggtc cgacactatg aatcggttgt ttgaaaaagt
     5581 tttgttggca ccgtctctga ataaaaatat tatccgctgc ttgtatagta ataacgatgg
     5641 aattatattt gtcaaaaaaa acaggctgag caacagacta ataattatgt aactgaataa
     5701 caagaaggca atcagcattt atgaatcaac aagatcgtaa gaaaaagacg gcatacaccg
     5761 gcaaagccaa aaaaacagcc ataaaacagg aaaaaattgc taataagcgt tggagttctt
     5821 atgtattgag cgcattgtta ataattacgg cggcaatatt tttccaggct ataaaatttg
     5881 attttctgac tacctgggat gataattttt atgtaacaga aaatcctgat ataaaagcgc
     5941 ttacatggtc aaatatccaa ttgctcttta caaaatccta tatgaatatg tatcaaccgc
     6001 tgaccatggt gttgtatgcg tttgagtacc agttaggagg cggtaatgcg gccatttttc
     6061 atttcgtaaa cgtactgttt catgtattga atacctggct ggtttatgtt tttattaaga
     6121 aaataacgaa aggtaaccag gtggttgcct tgttcactgc cgcatttttc ggggtgcatc
     6181 cgatgcatgt ggaatcggtt gcatggatat ctgaaaccaa agatgttttg tattcgtttt
     6241 tctttttgct gtcgctgata acatatcaca actatctcaa agacaacaaa ataaaacacc
     6301 tcatctgggc ctgcatgctg tttctgttgt catctatgag taagtctgca gcagtgatat
     6361 tgccgctggt gatgctgttg atggattatt acacaggcag gaaattcagc atgaaaatgc
     6421 tggttgaaaa aatcccgttc tttattattt ctctggtttt cggcttgatt tctctcaag
//
LOCUS       DAOSFP010000098         6438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10533_length_6438_cov_4.228889, whole genome shotgun sequence.
ACCESSION   DAOSFP010000098 DAOSFP010000000
VERSION     DAOSFP010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10533_length_6438_cov_4.228889"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PLI16_04140"
     CDS             complement(<1..361)
                     /locus_tag="PLI16_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH83778.1"
                     /translation="MKITFHGAAKEVTGSKHLITTNDGKKILLDCGMFQGKGLETDQE
                     NRALGFNPPDIDYVILSHAHIDHSGLLPYLVKNGFEGMIISTHATRDLCAVMLQDSGR
                     ITAHRSRVAWVEMIIPTH"
     gene            complement(445..2319)
                     /locus_tag="PLI16_04145"
     CDS             complement(445..2319)
                     /locus_tag="PLI16_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83779.1"
                     /translation="MKKVLFLAVTLAIGFSAFAQMKPAHISNDYRNVKAVKPARATHE
                     TMNFSTEVNQSVAPAPSKSVSEYQIGTTRYDLQSNACVANRIYLHSDGTIAATFTYGI
                     TEPSFADRGSGYNYYDGSAWGSTPSARLEANRSGWPSYAPLGATGEIVVSHNGSTGLL
                     INKRDPKGTGAWTQSVLVGPTTTGGTTALLWPRMITTGNTIHIIACTDQAESPAVWYY
                     EGLALALVYIRSTDGGTTWDAPRILPGMDSASIVSFVNAGFGGDSYGWANPVGDTIAF
                     FVNEGWMGMFVEKSYDGGYTWTKVNVFSVPAPTAWPTPKFCTTDGYGAVALDDQGKAH
                     VAFGRMRVNMSVQDLDSSFYYPYTDGLIYWNENMPVLDTTQLGDVDGLYAAGQWIGSM
                     LDYNGDTTINYPTPPAGELPFGKYFSSATSFPQILVDGSVVYVTYSQLREDKIDATST
                     KLLRHLFATKLEGGVWADGMDLTLDIIHDYDECVFGSLSQTSDDNLHIVYQADPIAGL
                     AVRGDETAYGDNNIYYIAFPKSDFAGVKENNTAVTMNIYPNPADEYSYIDINLTKSSK
                     VNLTVTNLVGQTVFTKTYGNLASGNQKLVVNVSNLNSGIYFFTIQAGNERLTKKIIVE
                     "
     gene            complement(2450..4504)
                     /locus_tag="PLI16_04150"
     CDS             complement(2450..4504)
                     /locus_tag="PLI16_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOH83780.1"
                     /translation="MKIRNLLFMGSIAILAASCSNNKQEAKNDEQPDTFKYKTEQFAD
                     VKILRYQIPGFNELSLNQKKLLYYLSQAALSGRDIIYDQNFKYNLIIRRTLEAIVDKY
                     NGDRNAEEFGKFMTYTKRVWFCNGIHHHYSSDKFLPEFSQEYFRQLVQNSPDAKFPLQ
                     KGETVEQLLEKIIPVMFDAGLYPKRINLDPSKDLIASSACNFYENLTQHEVEKYYEAI
                     IDTKNETPVSYGLNSKLVKEGGKIIEKPYKTGGLYSQAIEKIVYWLEQAKTVAENENQ
                     KAGLEKLIEYYNSGDLKTWDDYNVLWVKDLTSSIDYVNGFIEVYGDPLGMKATWESVV
                     NFKDVEATKRAETISQNAQWFEDNSPVDPRFKKKEVKGVSAKVITVVQLGGDCHPSTP
                     IGINLPNANWIRKQHGSKSVTMDNIMYSYDRSSMGSGSLEEFCYSGEEIERAKKYGYL
                     AGNLNTDLHECVGHGSGQLLPGVSSEALKNYHSALEEARADLFALYFTMDQKMIDLGL
                     VPSLEVAKAEYDTYIRNGLMTQLVRIELGKDIEQAHMRNRQLIAKWVYEKGEKDNVIE
                     KKIKEGKTFFVINDYQKLRGLFGELLKEIQRIKSEGDFTAGKEMVEKYAIKIDPVLHK
                     EVKTRYDKLKLAPYAGFINPVLVPVMKNNEIVDIKLKYPTDFAKQMMDYSKKYSFLPD
                     MN"
     gene            complement(4670..5656)
                     /locus_tag="PLI16_04155"
     CDS             complement(4670..5656)
                     /locus_tag="PLI16_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HOH83781.1"
                     /translation="MKQHTKIITIIIAFLLFAGLPTKSFSFGFYAHKKINRMAVFALP
                     PEMIGFFKKHIDYIIEHATDPDKRSRGVVGEAEKHYIDVELYGDSALSEIPKFWKAAI
                     EKFTEDTLRANGINPWWTSKMVYNLILAFKDEDLDRILFNAANLGHYIADACTPLHTS
                     TYYDGKILEQKGVHAFWETRLPELYSDNFNFLVGRAEYIDKPYEFIWQLVEESHACVD
                     TIFAVETDLRLNYPQDRIFVYDEKGTVIKKQFSKEYCDEFNKRTNNQVERRMRRAVYA
                     VASLWFTAWVNAGQPDLSRLENKEISKEHKKQLEETEKMWETGKPIGRPDPD"
     gene            complement(5653..6378)
                     /locus_tag="PLI16_04160"
     CDS             complement(5653..6378)
                     /locus_tag="PLI16_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOH83782.1"
                     /translation="MPCHSERNIEVCFSPALTNHFDITKKNVVLIDILRATSTICTAF
                     ARGVAEIIPVATEEEALKYKKSGHIVAGEHNGKTLAFADYGNSPLSLMAIDLKGKSMI
                     FCTTNGTKAVCAAAQARRLIFGSYLNFSAVAAFLEKNNKDTVLLCSGWNNNFSLEDTL
                     FAGALCSKLIDEKKFRLCDDAAMAALDLWNQAKNNLLCYIKKGTHYKRLIDLGFVDDI
                     KYCHTFDTTDKVPFLQKNTIVCI"
BASE COUNT         1646 a         1315 c         1454 g         2023 t
ORIGIN      
        1 catgggtagg tatgatcatc tctacccatg ccacgcgcga cctatgtgct gttatcctgc
       61 ccgaatcctg cagcataaca gcacataggt cgcgcgtggc atgggtagag atgatcatac
      121 cttcaaagcc attttttaca aggtagggca acaagcctga atgatcgata tgggcgtgtg
      181 aaagaatcac ataatcaatg tcgggcgggt tgaagcctaa ggcacggttc tcctggtcgg
      241 tttccaggcc tttgccctga aacatgccgc agtctaaaag gatttttttg ccgtcgttag
      301 ttgttatcag atgtttactt ccggttactt ctttagcggc gccgtggaaa gttattttca
      361 tatatttagt gttttattag caaaggtaat gaataaaaaa aacccgatat tgagtatcgg
      421 gtttttttta taaaaacagt tgtattattc aacgataatt tttttggtta acctttcgtt
      481 gcctgcctga atggtgaaga aataaattcc ggaattcagg ttgcttacat taacaactaa
      541 tttctgattg cctgatgcaa gattaccgta tgtctttgtg aaaacggttt gcccgacaag
      601 gttcgtaacg gttaaattaa cctttgaact cttggtcaga ttaatgtcaa tataagaata
      661 ttcatcagca ggattaggat agatgttcat ggtaacagcg gtgttatttt ctttcacgcc
      721 ggcaaaatca cttttgggga atgcaatata atagatgttg ttgtcgccat aagcagtttc
      781 atcgcctctg acagccagtc cggcgatcgg gtcggcctga taaacaatgt gcaggttgtc
      841 atcgctggtt tgtgataatg atccgaaaac acattcatca taatcgtgta taatgtccaa
      901 tgtcaggtcc attccgtcag cccaaacgcc accttcaagt tttgttgcga ataagtgtct
      961 taaaagtttt gtgctggtag catcgatttt gtcttcgcgc aattgcgaat aggtaacata
     1021 aactacactg ccatcaacaa gaatttgcgg gaaactggta gcagatgaaa aatattttcc
     1081 gaaaggcaat tcgccagctg gaggggtggg gtagttgata gtggtgtctc cattataatc
     1141 caacatgctg ccaatccatt ggccagctgc gtataatccg tcaacatcgc cgagttgagt
     1201 agtatccaaa acaggcatgt tttcattcca gtaaataagt ccatcggtgt aaggataata
     1261 aaatgatgag tcgagatcct gaactgacat atttactctc attctgccaa aagcaacatg
     1321 agcttttccc tgatcatcga gtgctactgc gccatatcca tcggtggtgc agaatttagg
     1381 ggttggccat gcggttggtg caggaacgga aaagacatta actttcgtcc aggtataacc
     1441 accgtcgtat gatttttcaa caaacatacc catccatcct tcattgacaa agaatgcaat
     1501 ggtgtctccc actggatttg cccatccgta tgaatcgccg ccgaagccgg catttacaaa
     1561 actaacaatt gatgccgaat ccattcccgg aagaatgcgg ggggcatccc aggtggtgcc
     1621 gccgtctgtt gatctgatat ataccagtgc cagcgcgagg ccttcatagt accatactgc
     1681 aggcgattct gcctggtcgg tgcaggcaat tatatggata gtgttgcctg tggtaatcat
     1741 acggggccaa agcagggctg ttgtaccgcc agtggtggtt ggtcctacca gtactgactg
     1801 tgtccaggcg ccggtacctt ttgggtcacg tttgttaatt aaaaggccgg tactgccgtt
     1861 atgagaaact acgatttcgc cggtggctcc cagtggtgcg tatgaaggcc agccgcttct
     1921 gttggcttca aggcgtgctg aaggagtaga accccatgct gaaccatcgt aatagttgta
     1981 gcccgaacct ctgtctgcaa aacttggttc ggtaatgcca taagtaaaag tagcggcaat
     2041 ggtaccatcg ctgtgcaaat aaattctgtt agcaacacat gcattggatt gcaggtcgta
     2101 acgggtggtt ccgatctgat attcggaaac actttttgat ggagccgggg ctactgattg
     2161 gttaacttct gtgctgaagt tcatggtttc gtgtgtggct cttgccggtt taacggcctt
     2221 cacatttctg taatcattcg aaatgtgcgc aggtttcatt tgtgcgaagg cggaaaagcc
     2281 aatcgccaat gttactgcta aaaataaaac ttttttcata aatctgggtt ttggttaata
     2341 attaatttta ttttattgat tatttcagtt gacaaatata taaaattttt tgtttgaaga
     2401 aaaaaaacat tttatatcta tccctctgat gtctggaaaa ctatgaaaat tagttcatat
     2461 caggcagaaa tgaatatttt tttgagtagt ccatcatctg tttggcaaaa tctgttgggt
     2521 attttagttt tatatcaact atttcattgt ttttcatcac gggtaccagc acggggttga
     2581 taaaccctgc ataaggcgcc agcttgagtt tgtcatagcg tgttttcact tctttgtgca
     2641 gcaccggatc gatttttatg gcatattttt ccaccatttc tttgccggca gtaaagtcac
     2701 cttcggattt gatacgttga atttctttga ggagctctcc gaaaagtccg cgaagtttct
     2761 ggtaatcgtt gatgacaaaa aaggtttttc cttctttaat cttcttttct attacgttgt
     2821 ctttttcgcc tttctcatac acccatttag cgatgagctg gcggttgcgc atatgggcct
     2881 gttcgatatc tttgcccagt tcaattctga cgagctgtgt catcagtccg ttgcgtatgt
     2941 atgtgtcgta ttctgctttg gccacttcga ggctgggaac aaggcccagg tcaatcattt
     3001 tctggtccat ggtaaagtat aacgcgaaaa gatctgcacg ggcttcttcc agagctgaat
     3061 gatagttttt caaagcttcg gagctgactc ccggcagcag ttgtcccgaa ccgtgcccaa
     3121 cgcattcgtg caggtcggtg ttcaggttgc cggcaaggta tccgtatttt tttgcgcgtt
     3181 caatttcttc tccggaataa cagaattctt cgagggagcc actgcccatg gatgaacggt
     3241 cgtaggagta catgatgtta tccatggtta ccgatttcga cccgtgctgt ttacgtatcc
     3301 aatttgcgtt gggcaggttg atgccgatgg gcgtactggg atggcaatct ccgccaagtt
     3361 gtaccactgt gattactttt gctgaaacgc ccttgacttc tttcttttta aaacgcgggt
     3421 caacggggga gttgtcttca aaccattgtg cgttttggct gatagtttct gctcttttgg
     3481 tggcttcaac atccttgaag ttgaccactg attcccaggt ggctttcata cctaacggat
     3541 cgccatatac ttctataaag ccatttacat aatcaatgga tgaggtcaga tcttttaccc
     3601 acaaaacatt atagtcatcc caggttttga ggtcgccgga attgtaatat tcaatcagtt
     3661 tttccagacc ggctttttga ttttcatttt cagccaccgt tttcgcctgc tccagccagt
     3721 acactatttt ttcgatagcc tgtgaataaa gaccacctgt tttatatggt ttttcaataa
     3781 ttttccctcc ttcctttacc agtttggaat ttaatccgta agatacgggg gtttcatttt
     3841 tggtatcaat gatggcttca tagtactttt caacttcatg ttgagtcaag ttctcgtaga
     3901 aattgcaggc cgatgaagca atcaggtctt tggagggatc aagattaatg cgtttgggat
     3961 ataatccggc atcgaacatt acagggataa tcttttcaag taactgctca acggtttcgc
     4021 ccttttggag tgggaatttt gcatccggag agttttgaac cagttgcctg aaatattcct
     4081 gcgaaaactc gggtaaaaat ttgtcgcttg agtaatggtg gtggatgccg ttgcaaaacc
     4141 aaacacgctt ggtgtatgtc ataaatttac caaactcctc agcgttgcga tctccgttgt
     4201 atttgtcaac tatagcctcg agcgtgcggc gtataatcaa gttgtactta aaattctggt
     4261 cataaataat gtctcttccg ctcagtgccg cctggctgag atagtagagc agcttcttct
     4321 ggtttaacga caattcgttg aatccgggaa tctggtagcg cagaatttta acatcggcaa
     4381 actgttccgt tttgtatttg aaagtgtccg gctgttcatc gttcttagct tcttgtttgt
     4441 tgttgctgca tgaagcagct aatatggcaa tagaacccat aaataataaa tttctgattt
     4501 tcatggtaat gaattaattt tttaggacaa acataaggaa aaaaattttt gcttacaatc
     4561 aggggacgac ttttaacaat attttaatat gtatgtcgtt gcgatatgcg aaaagactgc
     4621 aattatcggg gtacaggtaa gaatggtgtt cccgtcgagt tgtcttatat caatccggat
     4681 caggacggcc aatgggtttg cctgtttccc acattttttc ggtttcttcc agttgttttt
     4741 tatgttcttt ggagatttct ttgttttcca aacggctcag gtcgggttgc ccggcgttta
     4801 cccaggctgt gaaccatagg cttgcaacag cataaaccgc cctgcgcata cgcctttcca
     4861 cctgattatt ggtgcgtttg ttaaattcgt cgcaatattc ttttgaaaat tgttttttta
     4921 ttaccgtgcc tttttcatcg taaacaaaaa ttctgtcctg cggatagttt agacgcaaat
     4981 cggtctcaac agcaaaaatc gtgtccacgc aggcatggct ttcttccacc aattgccaga
     5041 taaactcgta gggcttatca atgtattcag cacgtccaac caggaagttg aagttgtcgc
     5101 tgtatagttc gggcaggcgt gtttcccaga atgcgtgaac tcctttttgc tcaaggattt
     5161 taccgtcata atatgtactg gtgtgcagag gtgtgcacgc atcggcaata tagtgtccaa
     5221 ggtttgccgc gttgaaaagg attctgtcga gatcttcatc tttgaaagcc agtataaggt
     5281 tgtacaccat tttgctggtc caccagggat tgatgccatt ggccctcagg gtatcttcag
     5341 taaatttttc aatggctgct ttccagaatt ttggaatctc cgacaaagcg ctgtcgccat
     5401 ataattcaac atcaatatag tgtttttccg cttctccaac cactccccgc gaacgtttgt
     5461 cgggatcagt agcgtgttca ataatgtagt ctatatgttt tttgaaaaaa ccgatcatct
     5521 cgggcggaag agcaaaaacc gccatacggt tgattttttt gtgggcataa aatccaaaag
     5581 agaatgattt cgtgggaagg cctgcaaaca ataaaaaggc gatgattatt gtgatgattt
     5641 ttgtgtgttg tttcatatgc aaacaatggt gtttttttgg agaaagggta ctttgtctgt
     5701 tgtgtcaaag gtatgacaat attttatatc atcaacaaaa cctaagtcga taaggcgttt
     5761 gtaatgtgtg ccttttttta tatagcacag caggttgttt tttgcctgat tccatagatc
     5821 caatgcggcc attgcagcat cgtcgcaaag gcggaatttt ttttcatcaa taagtttgct
     5881 gcacaaagcc cctgcaaaca gggtgtcttc aaggctgaag ttattgttcc accccgaaca
     5941 taataatacg gtgtccttat tatttttttc gagaaaggct gccactgctg aaaaattgag
     6001 ataggagcca aagataagcc gcctggcctg tgctgcggca caaacggctt tggtgccatt
     6061 agtggtgcaa aaaatcatgg atttaccttt tagatcaata gccatcaaac tcaaaggaga
     6121 attgccgtaa tcggcaaaag ctaaggtttt gccgttgtgt tcgccggcaa cgatatggcc
     6181 tgatttttta tatttcaggg cctcttcctc cgtggcaacc ggaataatct ctgcaacacc
     6241 ccgggcaaaa gccgtgcaga tggtagaagt ggcacgaagg atgtctataa ggaccacatt
     6301 tttttttgta atatcaaaat gattggtcag ggcgggagag aagcagactt cgatatttct
     6361 ttcagaatga caaggcatta tgttggtctt ttcagtgtaa caaaattaca aaaaaatgtg
     6421 agaggggaag gctaaata
//
LOCUS       DAOSFP010000099         6418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10592_length_6418_cov_4.119598, whole genome shotgun sequence.
ACCESSION   DAOSFP010000099 DAOSFP010000000
VERSION     DAOSFP010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10592_length_6418_cov_4.119598"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLI16_04165"
     CDS             complement(<1..614)
                     /locus_tag="PLI16_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOH83783.1"
                     /translation="MENYRNILVPYDFNEQAEIALSQSYNLARMLKLDIMLLYVHEET
                     KMLGKLFSADQSDDMVKKIEAELAAFAEQKSKETGLTINHMVARGKVYAKITEVAEMV
                     QAQFIILGKESSINTIEPTVGANTSRVIRNAKCPVITISADMHYNGCRSILLPIDLTQ
                     ESRQKVKWAIELAKMFNSTVKVLTILWSVNHKDIKNQLLTKMAQV"
     gene            757..1635
                     /locus_tag="PLI16_04170"
     CDS             757..1635
                     /locus_tag="PLI16_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006951604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOH83784.1"
                     /translation="MKTYFEESELILTAEKKIYHLNLHPDEIAGNIIVVGDPGRVPAV
                     SKHFDSIDVKVQNRELVTHTGYLNNMRITAMSTGMGPDNIDIVVNELDALANIDLNQR
                     TPKSGHRKLNIIRLGTSGAMQGDIPVDSFVASAYGLGIDGVLNFYKKTSEIVDKEMND
                     EFIRQTNWPEFLAKPYIVKASGDLLNKVAFDMIQGVTATAPGFFGPQGRELRGELAIP
                     DLNERLTGFAFNKNRITNFEMETSALYGLGKILGHDTLTICVIIANRIAKQFSKDYKP
                     LMEKLITTVLERLTAR"
     gene            1711..2670
                     /locus_tag="PLI16_04175"
     CDS             1711..2670
                     /locus_tag="PLI16_04175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOH83785.1"
                     /translation="MRKIYCFGEAVYDIIFKDEKPVEAKPGGAMLNVAISLGRLGLPV
                     CFVGDFANDRVGNIIKKFLEENNVDTSYITMYTNAKSRIALAFLDEKNNADYSFYKIR
                     IEDKPYIRFPELQPDDLLMFGSYYAIKPEIRSMVSEFIHNCNNKGAIVVYDPNFRLAH
                     LKMLDQLKPFIEENIAVADITKGSDEDFRNIFHTEDAEKTFSVLDHLGCNSMIYTRNR
                     YGVDLHTADYQHHYDAIGVKPVSTVGAGDTFTAGMAYWLYKNNICKSNLRNLSPEKYE
                     QMINTAILFATNVCKSYDNYISFEFINELKESFITQNSNNDKK"
     gene            2803..3372
                     /locus_tag="PLI16_04180"
     CDS             2803..3372
                     /locus_tag="PLI16_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH83786.1"
                     /translation="MNPGKDMKEMIKLCREGNSFAQEKLYKMYYGAMMNICLRYAKNS
                     DDAVEILNTGFLKVFQNMDKYQGDGEYLSAWIRKIMVNTALDRIRFEKNRYNNTVPLN
                     INNDSVHIENDAISRLGEADLIRLIEELPPASRAVFNMYVFENYNHKEIGDKLNITES
                     TSQWHLLNARRILMNRIQKLNSKKAIING"
     gene            3365..5092
                     /locus_tag="PLI16_04185"
     CDS             3365..5092
                     /locus_tag="PLI16_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOH83787.1"
                     /translation="MDKKNQIDDLFHKKLGEYEPPYVPEHWQMMKSALANPGNTGLKT
                     TGKSISGIIMIISAVFLITSGIVTYIALERSRQAELASGQLTTSSAISNSQLTDQNSF
                     NHQVVTPKDTKDLTEAQEPQPAERLNNVSGTNNLQAVNRPSNEKTTAQNHTKSTLPTK
                     NNNKTDNNRSNSAAVKVKMPDNNSDNFVLTEKEYPVVQKADEANFVNGVKVDYPNLGE
                     EKGASLNNVAITDKDKLFAGYSEAELDTTLSEENQGQSTVMDDYNTKQANAKENKRQA
                     NKNKNQIKKNLEAMPDFKVGVVNAIALNPAYTGYNQRHTIAVSTMVYKPLYRPGNNFN
                     VPFEYGFAYDFNFGKRKNCGLGINYQRFIGAAEGIMNVDLTFSYRFNLAKYHNLRVGA
                     SLSYFTSGINSDNLSFPDMIDANHGFVFGTSEAFPGKTNKNSMDIGLGVWYSWKSLYA
                     GVSAMHLTSPNLGIISEYNIPREYMLSAGYNYSINDYFGMLPVAELRYNEKTFNFSPG
                     MLFTYKKWLLFGAEFRNLKDAGLVLGFNMKDNVIINIRSGIPMSKVLMNNFGIIDYAG
                     VNVRLQFGN"
     gene            5111..5707
                     /locus_tag="PLI16_04190"
     CDS             5111..5707
                     /locus_tag="PLI16_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH83788.1"
                     /translation="MKKLFSPALLIFILIGVFISCKKQDVPPSETLYDTLKVNSAYSV
                     FTMGENTVLTLDATAPGAVAYQWMPDFYTTPMITITQEGNYSVKVTTHDNEFDYAVLV
                     LFSGSDCYIPNSFSPNNDGVNDYWHPYFANTSSGNFLLNIYDNENLKLFSSNDVDAKW
                     DGYHNGSLMPAGYYYYVLSYQTIDGETKNRNGMIQLIL"
     gene            complement(5743..>6418)
                     /locus_tag="PLI16_04195"
     CDS             complement(5743..>6418)
                     /locus_tag="PLI16_04195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HOH83789.1"
                     /translation="EKKLTNGTIVGGTIANFSSYGPTYDGRLKPDISAPGVGVVSSVS
                     SFTNQALSTPSASINFGGRTYDFVPFSGTSMSAPMVTGIVALMLQANPTLTPQQIKEI
                     IIETAREDNRTGNIPDTGSYVWGWGKVHAWRAIKTAYAGISEPEGDTEQVLVWPNPAK
                     EVVYLMNETATGPMTVNIYNTFGRLVASQTITQNRLNVGHLSAGIYFLEIISKNNSSM
                     VKLVKQ"
BASE COUNT         1952 a         1298 c         1377 g         1791 t
ORIGIN      
        1 acctgggcca tttttgtcag tagctggttt tttatgtctt tgtggtttac cgaccataaa
       61 atggtgagaa ctttcacggt agagttaaac atcttggcga gttctattgc ccatttaact
      121 ttctggcggc tttcctgtgt caaatcgatg ggtagcaaaa tactgcggca tccgttgtaa
      181 tgcatatcgg cgctgatagt aattaccgga catttggcgt tacggattac cctggaggta
      241 ttggcgccta cagtgggttc aatggtatta atactgcttt cttttcccaa aattataaat
      301 tgggcctgta ccatttccgc tacttcagtg atttttgcat aaactttgcc acgggccacc
      361 atgtggttaa ttgtcaggcc ggtttctttt gatttttgtt cagcaaaggc ggcaagttcg
      421 gcctcaattt ttttaaccat gtcgtccgac tgatcagccg aaaaaagttt tcctaacatc
      481 ttggtttctt catgtacgta aagtaacatg atgtcaagtt ttaacatacg tgcaagattg
      541 taggactggc tgagggctat ttcggcctgc tcgttgaaat cataaggaac taaaatgttt
      601 ctgtaatttt ccataaatat ttataatttt gtgcagtttg taaaaatagt aataaaaacc
      661 cgctaaaaca aaaatgattt ttattaaccg ggcaaaaata attaattttt aaaattgttg
      721 ttaagcggcc gcaaagatag atttaaattt tattttatga aaacatattt tgaagaatct
      781 gaattgattt taactgctga aaaaaagatt tatcacctga atctgcaccc tgacgaaatt
      841 gccggtaaca ttattgtggt gggcgacccg ggcagagtac ctgcggtttc gaaacatttc
      901 gacagcatag atgtaaaagt tcaaaacagg gaactggtta cgcataccgg atatttgaat
      961 aatatgcgaa tcactgcaat gtctacgggt atgggccccg acaatattga tatcgtggtg
     1021 aacgagcttg acgcccttgc caatatcgat ctgaatcaac gaacacccaa atccggacac
     1081 cgaaagttga atatcatccg cctgggaact tccggtgcca tgcagggaga tattcccgtg
     1141 gattcatttg tggcctctgc ctatggattg ggtatcgacg gggtgttgaa tttttataaa
     1201 aaaacatcgg aaatagtcga taaagagatg aatgatgaat ttatcagaca aaccaactgg
     1261 ccggaatttc ttgcaaaacc atacatcgta aaagcttcgg gtgatctgct caacaaagta
     1321 gcctttgata tgatacaggg tgttactgcc actgcaccag gattctttgg cccgcaggga
     1381 agagaactcc ggggtgaact ggccattccc gacctcaacg aacggctgac gggttttgca
     1441 ttcaacaaaa acaggattac caatttcgag atggaaacat cggccctgta tggcctggga
     1501 aaaattctcg ggcatgatac cctgaccatt tgtgtgatta ttgctaaccg tattgccaaa
     1561 cagttcagca aagattacaa gccgttaatg gaaaaactga taactaccgt actggaaaga
     1621 ttgacagccc gttagcacgc ggctgagaat tattaattca acatcttagt tttttcattt
     1681 ttaaactgta tttttgaaaa caaaaaatac atgagaaaaa tctattgttt tggcgaagcc
     1741 gtttacgata taatctttaa agatgaaaaa cctgtagaag ccaaacccgg cggcgccatg
     1801 ctcaacgtgg ccatctcgct cggacgcctg gggttgccgg tgtgttttgt gggagatttt
     1861 gctaacgaca gggtgggcaa catcatcaaa aaatttcttg aagaaaacaa tgtagatact
     1921 tcttacatta ccatgtacac caatgccaaa tcgcgtatcg ccctggcttt tctggatgag
     1981 aaaaataatg ccgactattc attttataaa ataaggatag aagataaacc ttatatcagg
     2041 ttccctgaat tgcagcctga tgatttgctg atgttcggat catattacgc cataaagcct
     2101 gaaatcaggt ctatggtttc tgaattcatc cacaattgta ataataaagg cgccatcgtg
     2161 gtgtatgacc ctaacttcag gttggcgcat cttaaaatgc tggatcagct caaacccttt
     2221 atcgaagaaa atattgctgt tgctgacatc accaaaggat cggacgaaga tttcagaaac
     2281 atttttcata cggaggatgc agaaaaaact ttttcggtgc tggaccacct cggatgtaac
     2341 tccatgattt atacccggaa tcgctatggg gttgatctgc atacagctga ttatcagcat
     2401 cattatgacg ctattggggt gaaaccggtg agtaccgtgg gcgccggtga tacgtttacg
     2461 gcaggcatgg cttattggct atacaaaaac aatatctgca aaagcaattt gcgtaacctc
     2521 tctcctgaaa aatacgaaca aatgattaat actgctatcc tttttgctac caatgtgtgc
     2581 aagagttatg ataattatat ttcatttgaa tttattaatg aattgaagga gagtttcata
     2641 acccaaaaca gtaataacga caaaaaataa attgtttttc gtatatttgt ataaaatact
     2701 gggtgataga gatagtagtc ataaagaaat atcagacggt aaaaaaatca tcccgtaccc
     2761 aagtttttat gaaaacatgc gttttataat taaagtgagc atatgaatcc cgggaaagat
     2821 atgaaggaga tgataaaatt atgcagggaa ggaaattcat ttgctcagga aaagttatat
     2881 aaaatgtatt atggtgcgat gatgaatatt tgcttgagat atgccaagaa tagcgatgat
     2941 gctgtggaaa ttctgaatac tggctttctg aaagtttttc agaatatgga taagtatcag
     3001 ggcgatggag agtacctcag cgcctggata cgcaaaataa tggttaatac cgcgctggac
     3061 agaatacggt ttgaaaaaaa tcgctacaat aatacggtac ctctcaatat taacaacgat
     3121 tcggtacata tcgaaaacga cgctatcagc aggcttggcg aagccgacct tatcaggttg
     3181 atagaggaac tgccaccggc aagccgcgcc gtattcaata tgtatgtctt cgaaaactat
     3241 aaccataaag aaatcggtga taaactgaac atcaccgaaa gtacctccca gtggcattta
     3301 ttgaatgccc gccggatact aatgaaccga atacaaaaac ttaattccaa aaaggctatc
     3361 ataaatggat aagaaaaatc aaatagacga cttattccac aaaaaacttg gcgagtacga
     3421 gcctccctat gtgcccgaac actggcaaat gatgaaatcg gcactggcta acccggggaa
     3481 taccggcttg aaaactaccg gaaaaagtat ctccggcatt ataatgatta tctccgcagt
     3541 atttttaatt acttcgggta ttgttactta tatagcactg gaaagatcaa gacaggccga
     3601 acttgccagc ggacaactga ctaccagttc agccatttcc aacagccagc ttaccgatca
     3661 aaattctttc aaccaccagg ttgtgacgcc aaaggataca aaagacctga ccgaagcaca
     3721 agaaccccag cctgcagaac gactaaacaa tgtgtcagga actaataacc tacaagccgt
     3781 aaacagacca tccaacgaaa aaacaactgc tcaaaaccat actaaatcta ctcttccaac
     3841 taaaaacaac aacaaaaccg ataataatag gtctaattcc gcagcagtaa aggtgaaaat
     3901 gcctgataat aattcagaca acttcgttct cacggaaaaa gagtatcctg tagtacaaaa
     3961 ggctgatgag gcaaactttg tgaatggggt gaaagtggac tatcccaatt taggtgaaga
     4021 aaaaggtgct tctctaaaca atgttgccat cacggataag gacaagcttt ttgcgggcta
     4081 cagcgaggca gagctggata ccaccctttc agaagaaaat cagggacagt ccacagtgat
     4141 ggatgactat aatactaaac aagccaacgc taaagaaaac aaaagacaag ccaacaaaaa
     4201 caagaatcag attaagaaaa acctcgaagc catgccggat tttaaggtgg gtgtggtaaa
     4261 tgccatagcc ttgaatcctg cctatacagg gtacaatcag cggcatacta tcgccgtttc
     4321 caccatggta tataaaccat tgtacaggcc tggaaacaat tttaacgtgc cattcgagta
     4381 tggttttgct tatgatttta acttcggaaa aaggaaaaac tgtggattgg gcatcaatta
     4441 ccaacgtttt atcggtgctg cagagggaat tatgaatgtg gaccttactt tttcatatcg
     4501 ctttaacctt gccaaatacc ataacctgcg ggtgggcgct tccctgtcgt attttaccag
     4561 cggcataaac tccgacaacc tcagtttccc ggatatgatc gatgctaacc atggttttgt
     4621 ctttggcact tccgaagcat ttcccggcaa gacaaacaag aattccatgg atataggact
     4681 cggcgtgtgg tatagctgga agtcgttgta tgcaggagtt tctgctatgc acctgacttc
     4741 gccaaatctg ggtatcatca gcgagtataa cataccccgc gaatatatgc tgtcagcagg
     4801 atataactat tccataaacg attactttgg catgttgcct gttgcagaat taagatacaa
     4861 cgaaaaaaca tttaattttt cgcccgggat gttatttacc tacaaaaaat ggctgctttt
     4921 tggtgctgag ttccgtaatc tgaaagatgc cgggctggtg ctgggattta acatgaaaga
     4981 taatgtcatt atcaatatcc gcagcggaat tcctatgagc aaagtactca tgaataattt
     5041 cggaattatt gattatgccg gagttaatgt gcgtttgcag tttggcaatt aatggtaata
     5101 gtttttcttt atgaaaaagt tattttcgcc tgctttattg atatttatcc tgataggagt
     5161 ttttatttcc tgtaaaaagc aggatgtgcc tccatccgaa acgctttatg atacattaaa
     5221 agtaaattca gcatatagcg tatttaccat gggcgaaaat accgtgctga ctctggacgc
     5281 taccgctccc ggtgcggtgg cctatcagtg gatgccggat ttttacacta cgcctatgat
     5341 tacaattact caggaaggta attattcggt gaaggtaacc acccacgaca acgaattcga
     5401 ttatgcggtg ctggttcttt tttcgggcag cgattgttat attcccaatt ctttttcgcc
     5461 caacaacgac ggggtgaacg attactggca cccgtatttt gcgaacacct cctccggaaa
     5521 ttttttgctc aacatttatg ataatgaaaa ccttaaactg ttttcatcaa acgatgtgga
     5581 tgccaaatgg gatggctatc ataatggatc cctgatgcct gccggttatt attattatgt
     5641 gctgtcgtac caaaccattg acggggaaac caaaaaccgc aacgggatga ttcagttgat
     5701 tctctgacaa ccaattagtt caaaacgcaa gaccgggcat gtttactgtt ttaccagttt
     5761 taccatactg gaattgttct tcgaaataat ttccaggaaa taaattccgg cagacaggtg
     5821 gccgacattt agcctgttct gtgttatggt ttgcgaagct accaggcggc cgaatgtgtt
     5881 atatatatta acagtcatcg ggccggtcgc agtttcattc ataagatata caacttcttt
     5941 ggcagggttt ggccaaacca acacctgctc agtatctcct tcaggttcgc tgatgcctgc
     6001 atacgctgtt ttaattgctc tccaggcgtg cacttttccc cagccccata cgtagcttcc
     6061 ggtgtcggga atgtttccgg tacggttgtc ttcacgggct gtttcgatga ttatttcttt
     6121 tatctgttgc ggtgtgaggg tggggttggc ctgcaacata agcgccacga tgcctgtaac
     6181 cataggtgcc gacatggatg tccccgaaaa aggaacaaaa tcataggttc ttccaccgaa
     6241 atttatggag gccgaaggcg tagacagggc ctgattggtg aatgaagata cagaagaaac
     6301 aacgccaacg ccaggggcag agatgtccgg tttaagccgg ccgtcgtaag tagggccata
     6361 gcttgagaaa ttggcaatgg tgcctccgac aatggttccg ttggtcagtt ttttttcc
//
LOCUS       DAOSFP010000100         6401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10623_length_6401_cov_3.551686, whole genome shotgun sequence.
ACCESSION   DAOSFP010000100 DAOSFP010000000
VERSION     DAOSFP010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10623_length_6401_cov_3.551686"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3754
                     /locus_tag="PLI16_04200"
     CDS             <1..3754
                     /locus_tag="PLI16_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH83790.1"
                     /translation="SLNRESLSLSRGEAQRLRLANQAVSGLRNVLYILDEPSAGLHPS
                     EHRGLLNVIRMLVSTGNTVMLVDHDEQSIREADWIIDIGPGAGKAGGEILYNGPAADF
                     FACDQPQSITRKYLSGKQILLPEKHKTGKLDWFTVVQANKNNLRNISPRFLINAFNVI
                     TGVSGSGKTSLVNFMLENTCRQKKGGTEIFGKIIHIDSSPIGRTPKSNPATYTGLSDH
                     VRDLLASLEESRKKGFKKGQFSFVVKGGRCETCGGAGVQQIGMHFLGNVEVVCDACNG
                     RRFNEETLEIKYKGLNIYDILELTVDEACDFFREHKKISAIAEILSSLGLGYLKLGQP
                     STTLSGGEAQRIKLAAELSRSAAQHTLYILDEPTTGLHMADVEILLKALKKLIAKGHT
                     LLCVEHDPALILHADWIVDLGPGSAADGGNIVAEGFINEVINHQQSVTAHELRKYLER
                     DATFSRTQNLPCSKETIEAPISICGVETNNLKNIDISFPIDKISAVTGVSGSGKSSLV
                     FGSLYAESHRRFLEGMSSYMQQFTKKAGIPIVKQSHGLVPSIALQKKNTIKNPRSIIS
                     TYTGLYDLYRLMFSRLGKSDSDTDRPLSTAFSFNSEEGACPECRGLGSITVCDAEKLI
                     TNPEKPLTAGAMDNSKTGKFYGDPHGQYIATLLTIGRLHGVDFAVPYQSLTAQTKDLA
                     MLGCGDTIFEVDWHYKRGALEGVHKLKTAWPGFLKLVETEYIRKHSDARREVILDLMK
                     ALQCNHCKGFRLKPDMLHYTINGLHIGQLTAMSADEAIEWFSGDFTALFQTSLEKEAA
                     GVFRTNIMERLDALVKSGLGYIATDRIVGTLSGGEFQRLQLAGLVRAPLTGVMYILDE
                     PSFGLHHSDLIPISDLIQKLNANGNTIIMVDHSPLLLEKSDFLIKMGPGAGSKGGQIV
                     FSGKMEEYPPETTYHTDIKTNHTKPDTGLKIEKAHANNLRNINIELPKESMTVISGVS
                     GSGKTSLLDKVIFGSYQKHTPTFCEDISGFENFNSLIYIEQPTPETKHSTTTGEKLGI
                     SEIIAGIFSGADTSMKQHYKPVHFTRGSRESRCPGCEGSGINTVSMDYFTDVVTPCER
                     CGGSGYKDEIMQITVEDKNIFEIHQLTFTELAEFAGRYLHGKPKNKAFHILQLLEKTG
                     LGHLSSGRLLKTLSTGELQRIKLVAGLLTKTADNNLILLDEPTGGLHPADTAQLLTLF
                     EELLLAGNTLVCVSHEPMMLQAASVVIELGPGGGKQGGMIVSHTTK"
     gene            3817..5997
                     /locus_tag="PLI16_04205"
     CDS             3817..5997
                     /locus_tag="PLI16_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HOH83791.1"
                     /translation="MKVCIAEKPSVAKEIARILQANSRKDGYYEGNGYRITWTFGHLC
                     TLKNPDDYTPEWKFWKLNTLPMIPPRFGIKLIENDGIKKQFNTIKNLVSECSEVINCG
                     DAGMEGELIQRWVLTKAGCSKPVKRLWISSMTDEAIKEGFEKLRDGRDFDRLYSAGNA
                     RAIGDWLLGMNASRLFTLKFADGKGVLSIGRVQTPTLALIVRRYEEIQNFVPKPYWEI
                     KTNYREVIFTSTIGRFSTAEEAAQVIEEIKDNLFEIVSFEQKKGNEAPPKLFDLTALQ
                     VECNKKFSFTADDTLKYIQNLYEKKMVTYPRVDTTFLPNDMYPKIEGILRKMEPYANF
                     TAPLLDKPIRKTKKVFDDAKVTDHHAIIPTGVRPTGLILEEKKVYDLIARRFIAAFFP
                     DCVVSNTTVIGKVDRHEFKATGKVILEDGWRALYPRKENEKDGENDAGEEQIMPHFEK
                     GEKGPHKPDLLEKQTQPPKMHTEATLLRAMETAGKQVEDEELRELMKENGIGRPSTRA
                     NIIETLFKRKFIIREKKNLLPTQTGIELIHTIRNELLKSAELTGQWEKKLRQIEKGEY
                     NPQNFLDEMKEMVKQLVEEVKRENGKTIAIEQEAKPEEKEAVATTKPQPAKKEPSSEK
                     KVAAKTVDIATLTCPKCNMGSILKGKAAYGCSEYKNGCTFRVLFEQYGVTLTDKQIFS
                     LIQKGKTPKIKGLNVDGAKKDALLTFDGLYQVVAKRDKAEGKEE"
BASE COUNT         2009 a         1450 c         1466 g         1476 t
ORIGIN      
        1 ttcgctaaac cgcgaatctc tttcgttaag cagaggtgag gcccagcgtt tacgtcttgc
       61 taatcaggca gtgagcggat tacgcaacgt gctttacata ctggacgaac ccagcgccgg
      121 ccttcaccct tcggaacacc gcggattgct caacgtcata cgcatgttgg taagcaccgg
      181 caataccgtg atgcttgtag atcatgatga acaaagcata cgtgaagccg actggatcat
      241 tgatataggc cccggcgcag gaaaagccgg aggagaaatt ttgtataacg gccctgcagc
      301 agactttttt gcctgtgacc agccccaaag catcaccagg aagtacctta gcgggaagca
      361 aatattgttg ccggaaaaac acaaaaccgg caaattagat tggtttacgg tggtgcaggc
      421 caataaaaac aacctcagga acatatctcc ccggttcctg atcaatgcct ttaacgtcat
      481 caccggagta tcggggagcg ggaaaaccag ccttgtgaat ttcatgctcg aaaacacctg
      541 ccggcaaaaa aaaggaggaa cagaaatttt tggtaaaata atacatatcg actcctctcc
      601 tatcggccgc acaccaaaaa gcaatcctgc aacctacacc gggttgtcgg atcatgtacg
      661 cgacctgctg gcatctttgg aggaatcccg gaaaaaagga tttaaaaaag gccagttttc
      721 gtttgtggta aaaggcggac gttgcgaaac atgtggtggc gccggtgtcc aacagatagg
      781 aatgcacttt ctcggaaatg tggaagtggt ttgtgatgcc tgcaatggac ggagattcaa
      841 tgaagagaca ctggagataa agtacaaagg attaaacatt tacgacatct tagaactaac
      901 ggttgacgaa gcctgcgact ttttcaggga acacaaaaag atcagcgcca tagcggaaat
      961 actgtcatca ctcggcctgg gttatctgaa actaggccag ccatccacca ccttgtcggg
     1021 aggcgaggcc cagcgcataa aactcgccgc cgagttgtcg aggtcggctg cacagcacac
     1081 cctgtatatt ctggatgaac caaccacggg cctgcatatg gccgatgtgg aaatactgct
     1141 taaagcgctg aaaaagctta ttgctaaagg acataccctc ttatgcgtag aacacgatcc
     1201 ggcccttatt ttgcatgcgg actggatagt ggacctgggc ccgggcagcg ctgccgatgg
     1261 cggaaatatt gttgccgaag gatttataaa tgaagtaatc aatcatcagc agtcagttac
     1321 ggcacatgaa ctccgaaaat acctcgaacg ggacgccact ttttccagaa cacagaacct
     1381 gccttgcagc aaagaaacga tagaggctcc catcagtatt tgtggcgtgg aaacaaataa
     1441 tctgaaaaac attgatatat cgtttcccat cgataaaatt tcagcggtaa caggagtttc
     1501 gggaagcggc aaatcttccc tggtttttgg ctcactttat gccgaaagtc accggcgttt
     1561 tctggaaggg atgtcatctt atatgcaaca gtttacgaaa aaagccggca tccccatcgt
     1621 aaaacaaagc cacggcctag tcccgtcaat cgcactacag aaaaagaata ctatcaaaaa
     1681 cccaaggtca ataatatcaa cctatacagg cctttacgac ctttaccggc tgatgttcag
     1741 caggctggga aaatcagatt ctgacactga caggcccctg tccaccgctt tttcttttaa
     1801 cagcgaagaa ggcgcctgcc ccgaatgccg tggcctgggc agtattacag tgtgcgatgc
     1861 agaaaaactg attaccaatc cggaaaagcc tttgacagca ggagcaatgg ataacagcaa
     1921 aacaggcaaa ttttacggcg atcctcacgg gcaatatatt gccacattac tcaccattgg
     1981 ccgcttacac ggagtagatt ttgctgttcc ttaccaatcc ctgactgcac agaccaaaga
     2041 tctggccatg cttggatgtg gtgatacaat ttttgaggta gactggcatt acaaacgagg
     2101 agctcttgag ggagttcaca agttaaaaac cgcctggccg ggatttttaa aacttgtgga
     2161 gaccgaatac atacgaaaac acagcgatgc ccgccgcgaa gtcatactcg acctgatgaa
     2221 agcactacaa tgcaatcact gtaagggatt caggctgaaa ccggacatgc tgcactatac
     2281 cattaacggc ctgcacatag gacaattaac ggcaatgagt gctgacgaag caatagaatg
     2341 gttttcggga gattttaccg cattgttcca aacaagcctt gagaaagaag ctgccggagt
     2401 gttccgcaca aatattatgg aacgccttga cgcattggta aaatcaggat tgggctatat
     2461 cgccacagac aggatcgttg gaacgctttc tggcggagaa tttcagaggc tacaacttgc
     2521 cggacttgtc agagcccctc tcaccggagt aatgtatatc ctcgatgagc cttcattcgg
     2581 cctgcaccac agcgacctga ttcctatcag cgatctgata cagaagttaa atgcaaacgg
     2641 caacactatc attatggtgg accactcccc cctgctgctc gaaaagtcag actttttaat
     2701 aaaaatggga cctggcgccg gaagcaaagg cgggcagata gttttttcag gaaagatgga
     2761 agaatatcct cctgaaacaa cttatcacac agatataaaa acaaaccata caaagcctga
     2821 cactggcctg aagattgaga aagcacatgc caacaatctg cgtaatataa acatcgaact
     2881 tcccaaagaa agtatgactg taataagcgg agtgagcggc agcgggaaaa ccagcctgct
     2941 cgacaaagtg atatttggca gctaccagaa acatacacca acgttttgtg aagatatctc
     3001 gggatttgag aatttcaaca gcctgatata tattgagcaa cccacacccg aaacaaaaca
     3061 cagtaccacc acaggcgaaa agcttggcat ttcagaaatc atcgcgggaa ttttttccgg
     3121 tgcagatacc agcatgaaac aacattataa accggttcat ttcacaagag gttcacgcga
     3181 aagccgttgt cccggttgtg aaggttccgg catcaataca gtaagcatgg attacttcac
     3241 tgatgtggtt acaccatgcg aacgatgcgg cggcagcgga tataaggacg agattatgca
     3301 gattacagtg gaggataaaa acatttttga aatccatcag cttacgttca cggaattagc
     3361 tgaatttgca gggagatacc tgcatggtaa gccaaaaaat aaggcattcc acattttaca
     3421 acttttggaa aaaacaggct taggacattt atccagcggc cgtttgctca aaacgctttc
     3481 gacgggggaa ttgcagcgaa taaagctggt tgccggcctg ttgaccaaaa cagcagacaa
     3541 caaccttatc ctgctcgacg agcccacagg gggcctgcac cctgccgaca cggcacagct
     3601 ccttacactt tttgaagaac tcctgcttgc cgggaatacc ctggtatgtg tttcgcacga
     3661 accaatgatg ttgcaggccg cttcggtagt cattgaactt ggcccgggcg gtggcaaaca
     3721 aggtggaatg attgtcagcc acactacaaa ataatttgat ccgctatcga aaatacgcct
     3781 gttttattaa atttgcattg aaaagcatcc cgcaccatga aagtttgtat agccgaaaag
     3841 cccagcgtgg ctaaggaaat cgcacgcata ttacaggcaa acagccgaaa agacggttat
     3901 tacgaaggta atggatacag gataacctgg acattcgggc atttgtgcac actgaaaaac
     3961 cctgatgact atacgcccga gtggaaattc tggaagttga acaccttacc gatgattccg
     4021 ccccggtttg gcataaaact catagaaaac gacggaataa aaaaacaatt caacaccata
     4081 aaaaatctgg tttccgaatg tagcgaagtc atcaactgcg gtgatgccgg catggaaggt
     4141 gaactgatac aacgctgggt acttaccaaa gccgggtgct caaaacctgt aaaacgtctg
     4201 tggatttcat cgatgaccga cgaagctatc aaagaaggtt ttgaaaaact cagggatggc
     4261 agagactttg accgcctgta ttcggccggc aacgcccgtg ccattggtga ctggctgctt
     4321 ggaatgaatg ccagccgcct ttttacacta aaattcgccg acggcaaagg tgttctttct
     4381 atcggaaggg ttcagactcc caccctggca ctcatcgtac gccggtatga agaaatccag
     4441 aatttcgttc cgaaacccta ttgggaaata aaaaccaatt accgtgaggt gatttttact
     4501 tcaacgatcg ggaggttcag tacggcagaa gaagctgcac aagttatcga agaaattaaa
     4561 gataatctgt ttgagattgt ttcattcgaa caaaaaaaag gcaacgaggc gcctcccaaa
     4621 ctgttcgacc tgaccgcatt gcaggttgaa tgtaacaaaa aattcagctt taccgccgat
     4681 gacaccctta aatacataca aaatctttat gagaagaaaa tggtgacata tccccgcgtg
     4741 gataccacat ttcttcctaa cgatatgtat cccaagattg agggtatttt gagaaagatg
     4801 gaaccctatg ccaatttcac tgccccgctg cttgacaaac ccatacggaa gaccaaaaaa
     4861 gtgttcgacg atgccaaagt taccgaccat cacgctataa tccccacggg tgttcggcct
     4921 acggggctga tactggaaga aaaaaaagtg tacgacctga ttgcacgccg cttcatagcc
     4981 gctttctttc ccgactgcgt tgtatcaaat acgacggtga tcgggaaggt ggaccggcac
     5041 gaattcaaag caacgggtaa agtgatactg gaagatggct ggcgggcgct ttatcccagg
     5101 aaagaaaatg aaaaggatgg cgaaaacgat gccggtgaag aacagattat gccgcatttt
     5161 gaaaaaggcg aaaaagggcc gcataaaccc gaccttctcg aaaagcaaac ccaaccgcca
     5221 aagatgcata ccgaagccac cctgctgcga gctatggaaa ccgccggtaa acaggtggaa
     5281 gacgaagaac tccgcgaact tatgaaggag aatggcattg gacggccatc cacccgcgcc
     5341 aacatcattg aaacactgtt caaacgaaag tttattatcc gcgaaaagaa aaacctgctg
     5401 cctacccaaa caggtataga acttatacac accatcagaa atgaattgct gaaatcggcc
     5461 gaactgaccg gacagtggga aaagaaactc cgccagatcg aaaaaggaga atacaatcca
     5521 caaaattttc ttgatgagat gaaagaaatg gtgaagcaac tggtggagga agtaaaacgc
     5581 gaaaacggca aaaccattgc cattgagcag gaagcgaaac ctgaagaaaa agaagctgtc
     5641 gcaaccacaa agccgcagcc agccaaaaaa gagccatctt ctgaaaaaaa agttgccgcg
     5701 aaaaccgtgg atattgccac cctcacctgc cccaaatgca atatgggaag catattgaaa
     5761 ggcaaggctg cctatggttg cagcgaatat aaaaacggat gcacgttcag ggtattattt
     5821 gaacaatatg gggtaacgct cacagataaa cagatattct cactgataca gaaagggaaa
     5881 actccaaaaa taaaaggtct gaatgttgat ggcgccaaaa aagatgccct actgactttt
     5941 gatgggctgt atcaggtggt ggccaagaga gacaaagcag agggaaaaga ggaataaacg
     6001 gcattttacc acacaacgtt caaaaatcca cttttttcag ttagtccaca acttttggca
     6061 ttgaaccatt tttaatgcgt aacctggatt tatttatttt ttattatatc ttgatttttt
     6121 tgtattttag cattgaatat tgcaatgaga taattaatgt taaagatcgg gtactatctt
     6181 gttgatcccc ggaatctttt cttctattga aacaatgtat ggtttgattt gtttttatat
     6241 gagaactttt tctttctttt gtattataag tttctgacag aattctcaaa gttttttacc
     6301 caagaaattt gtattcatta taggatgttg aagttagtta taaatacatt actgatgcgt
     6361 taatatttga ttgaaattaa ataaattaag ttgttcattg a
//
LOCUS       DAOSFP010000101         6375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10696_length_6375_cov_3.978797, whole genome shotgun sequence.
ACCESSION   DAOSFP010000101 DAOSFP010000000
VERSION     DAOSFP010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_6375_cov_3.978797"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="PLI16_04210"
     CDS             <1..1177
                     /locus_tag="PLI16_04210"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOH83792.1"
                     /translation="KDFDGVAYDTLVDIIDLAIDPRDNTHVFAASLYRGVVELKNGYI
                     TNVYGVNNSALELPFGSVYYWLGIGGVAFDANNNLWAVNTNCNNLLKVRRTDGTWLSY
                     STIPYLSQGNGGKVMVDQTGQKWVLLMNGAGLLVFNENGTWTDNSDNVTRLLTTSVGN
                     GNLPSNSVYSFAEDLDGQIWIGTDKGVAVFYDPENITLGGNFDAQLITVMQDGTAQHL
                     LAFEKVTAIAVDGANKKWFGTENAGVFLMSEDGQEQLFHFTEDNSPLLSNTITSIAID
                     EKTGVVYFGTSNGLVSFRAYATKGEEVFTNVYAYPNPVRENFSGMIGIKGLVKDATVK
                     ITDISGTLVYETLSEGGQAVWNGKNFHGESVRSGVYFVFCSSEDCTEKLVTKIMVIN"
     gene            1193..1921
                     /gene="recO"
                     /locus_tag="PLI16_04215"
     CDS             1193..1921
                     /gene="recO"
                     /locus_tag="PLI16_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOH83793.1"
                     /translation="MISTTKAIVLKTIPYSETSVVVKMYTELFGLKSFLIRGVRKKHA
                     RISANLLQPLSINEVVFVNKEKDQLHIPKEMASCYQFTSLPFDVTKSSQALFINELLY
                     RSIREEEANPEFFNFIFQNILLLDQLANTRGNLHIAFALHLTRYLGFYPLGNFSPQTP
                     YFLLKEGVFGKYKPADDFFLNQQEAEVFDRFLQTSVEDAVQIKLTASQRNTLLEKIIL
                     YYQIQHIGFGEIKSLAVLTQLFHE"
     gene            1940..3352
                     /locus_tag="PLI16_04220"
     CDS             1940..3352
                     /locus_tag="PLI16_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH83794.1"
                     /translation="MESYKLYVGGNFKETASKITVVNSYSNQVFAEVSLASANELETA
                     IKAAQDVKKTIAHLASFHRYAILMDIAGLLRTERQRLANVLAMEACKPVKLALAEVDR
                     AIQVFVVAAEESKRLPKEYISMDWTPAGQGREGLVKYFPVGLVAGISPFNFPLNLAVH
                     KIAPAIAAGCPIILKPSSSTPLSALELARLVDKTELPKGALSVLPMDRKTGNLLVTDE
                     RFNLLSFTGSPEVGWKMKSDAGKKKVVLELGGNAGVIITASADIGKAVGKCVAGGFSY
                     AGQVCIHTQRIFVHKSIVEDFTALFVEKVKQLRQGAPEHPETDISAMIDEKNAMRVES
                     WVNEAVAGGARLLCGGQRKGSFFEPTVISSAKNSMNICCCEIFGPVVVLEPYEDFEKA
                     VEEVNNSRYGLQAGVFTDSVAEMDYAFDNLEVGGVIINDVPTFRTDHMPYGGIKDSGL
                     GREGVKYAILDMMEPRLLVK"
     gene            3566..4684
                     /locus_tag="PLI16_04225"
     CDS             3566..4684
                     /locus_tag="PLI16_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83795.1"
                     /translation="MKKTYVLIMLIFSIGFTKANPISPPPIISELYIINDSTWFLEIV
                     FLQGNPFINYANLDGFRITTSMGTSYLKNGIPIINDSIIVITQDSLQSPLQIFRNGDF
                     ILIKDISGLQLDQIYFGNISGTQISAPLLGQSIVNLAHPCWDTYLNQPGTVNKLVKDN
                     HPTIGYSPFQPFSAIGIFTGVVYDSGHQPVAGIKIGNSHYIDLAPTNICANFYYFTLT
                     DSLGSFTSQEYSGSRYVELFFQSSSVLADSIINIEPDSINYYEFTIDTLLTGVQQNYF
                     QTGITLLCYPNPSTGETFISFSIPAGIHYSKAIIKIYDSNSEMVRILPVNTSNSQNNY
                     SIKWDGLCYNNATASGTYYCNLELDGRKSASNKIIIIK"
     gene            4681..5256
                     /locus_tag="PLI16_04230"
     CDS             4681..5256
                     /locus_tag="PLI16_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83796.1"
                     /translation="MKFIKISIFFSLILLHGIVCSQNLQFYREDLTFEIKDGFFYVNG
                     IYNFCNNGDKEIQQVLFYPFPVDSLYGRVDSINAFDVNAKSQNIILNITEKGFLFNIG
                     LNPYGIGKYKISYRQKLLKNKAEYILVSTQKWGAPFESSNYKLISYDNTRITATSYIP
                     DSIKQINDKGIYYWSKKDFMPDKNMIFYFDR"
     gene            complement(5501..>6375)
                     /locus_tag="PLI16_04235"
     CDS             complement(5501..>6375)
                     /locus_tag="PLI16_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOH83797.1"
                     /translation="YVDALPELINPRTGRIHTSYNQAVAATGRLSSNNPNLQNIPVRT
                     ERGREIRKAFVPRNEDYVLVSADYSQIELRVIASISEEANMIEDFRQGHDIHAATASR
                     VYNVALADVTKEMRRNAKMVNFGIIYGISAFGLAERLNIQRKEAAEIIEQYFIKYPAI
                     KKYMEDTISSARKKGYVETILGRRRYLRDINSANAVVRGYAERNAINAPIQGSAADII
                     KIAMINIYKQMTEMQTRSRMIMQVHDELVFDVYKPELETMLPLVQAQMQNAIIIKVPL
                     DVDISSGNNWLEAH"
BASE COUNT         1837 a         1328 c         1395 g         1815 t
ORIGIN      
        1 caaggatttt gatggcgtcg cttacgacac actcgtagat attattgatc tggctatcga
       61 tcccagggac aatacgcatg tgtttgcggc ttcgctatac aggggtgtcg tggagttaaa
      121 aaacggctat atcaccaatg tttacggggt gaataactcg gcgctggaat tgcctttcgg
      181 aagtgtttat tactggcttg gcatcggcgg ggtggctttc gacgcaaaca acaacctgtg
      241 ggcagtaaat accaattgca ataacctgct gaaggtgcgc agaaccgacg gaacctggct
      301 ctcctacagc actatcccat acctgtcgca gggtaatggt ggaaaggtaa tggtggacca
      361 aaccggccag aaatgggtgc tgctgatgaa cggcgccggg ttgctggtgt ttaacgaaaa
      421 cggaacatgg accgataata gcgacaatgt taccaggcta ctcaccacca gtgtgggcaa
      481 cggaaacctg ccaagcaact cggtgtacag ctttgccgaa gacctcgacg gacaaatatg
      541 gataggcacc gataagggag tggccgtgtt ttatgacccg gaaaatatca ctctcggcgg
      601 aaactttgat gcccagctga taacggttat gcaggacggc actgcacaac acttgctggc
      661 tttcgaaaaa gtaacggcca ttgcagtgga tggggccaac aaaaaatggt ttggaaccga
      721 aaacgccggc gtattcctta tgagcgaaga tggccaggaa cagcttttcc attttaccga
      781 agataacagc ccgttgttgt ccaacaccat taccagcatt gccattgatg aaaagaccgg
      841 ggtggtgtat ttcgggacct caaacggact ggtgtctttt cgtgcttatg ccaccaaagg
      901 cgaagaagtg ttcacaaatg tctatgctta tcccaatccc gtgcgcgaaa acttttcggg
      961 tatgattggc atcaaaggac tggttaaaga tgctaccgtc aagattaccg atatcagtgg
     1021 caccctggtg tacgaaacat tatcggaagg cggacaggcc gtgtggaacg gaaaaaactt
     1081 ccatggcgaa agtgtcagaa gcggcgttta ctttgtcttt tgcagcagcg aagattgtac
     1141 cgaaaaatta gtgaccaaaa ttatggtcat caattaagct gccacttttc ctatgatttc
     1201 aaccaccaaa gccattgtcc ttaaaacaat accgtattcg gaaaccagtg tcgtggtgaa
     1261 aatgtacacc gaacttttcg gattgaaatc ttttttgata cgcggtgtcc ggaaaaaaca
     1321 tgccaggata agtgctaacc tgcttcaacc ccttagtatc aacgaggtgg tctttgtcaa
     1381 caaagaaaaa gatcaactgc acattccgaa agaaatggca tcctgctacc agtttacctc
     1441 cctgcctttt gatgtgacaa aaagctcaca ggcattgttt atcaacgaac ttctataccg
     1501 ctccatcaga gaggaagagg ccaaccccga attttttaat ttcatttttc aaaacatatt
     1561 actgctcgac caactggcca atacccgggg aaacctgcac atcgcctttg ccctgcacct
     1621 gacacgttac ctcgggtttt atccccttgg aaacttttcg ccgcaaacac cttattttct
     1681 tttaaaagaa ggcgttttcg gcaaatataa gcctgccgat gattttttcc ttaaccagca
     1741 ggaggctgaa gtgtttgaca ggtttttgca gacatctgtt gaagatgctg tacaaataaa
     1801 acttacagca tcccagagga ataccctcct ggagaaaatt attctttatt accagataca
     1861 gcatattggg tttggagaaa taaaatcatt ggcggtgtta acccaacttt ttcacgaata
     1921 aaagccacaa acaaaaatta tggaatcata taagctttat gtcggaggta attttaagga
     1981 aacggcgagc aaaattactg tcgtgaattc ttattcaaac caggtatttg cagaggtttc
     2041 gcttgcttcg gcaaacgagt tggaaacagc tatcaaagca gcccaggatg tgaaaaaaac
     2101 tatagcacac ctggcttctt ttcaccggta tgctatcctg atggatattg ccggactctt
     2161 gcgtacagaa cgccaaaggc tggccaatgt cctggccatg gaggcctgca agcccgtgaa
     2221 actagctttg gctgaagtgg acagggcaat acaggttttt gtggtggccg ccgaagagtc
     2281 aaaacggctg ccaaaggagt atatcagtat ggactggact cctgcgggcc agggcaggga
     2341 aggcctggta aaatatttcc ccgtgggact ggtagcaggc atttcgcctt ttaattttcc
     2401 gctgaacctg gccgtgcata agatagctcc cgctattgcc gcgggctgcc ctattatcct
     2461 gaaaccttcc tcatccacgc cactttcggc gcttgagttg gcccgtttgg ttgataaaac
     2521 cgaactgccc aagggcgcat tgtctgtgtt gcctatggac aggaaaacag gtaatttact
     2581 ggttacggac gaacggttta atttattgag tttcacggga tcacccgaag taggatggaa
     2641 aatgaaaagc gatgccggaa agaaaaaagt ggtgctggag cttggcggta atgccggtgt
     2701 gataataacg gcaagcgctg atataggcaa ggcagtggga aaatgcgtcg cgggaggatt
     2761 ttcctatgcc gggcaggtgt gcatacatac ccagaggatc tttgttcaca aaagtatagt
     2821 tgaggatttt accgcgcttt ttgtagagaa ggtaaaacag ctcaggcaag gcgctcctga
     2881 acaccccgaa actgatatct ccgcgatgat tgatgagaaa aatgctatgc gcgtagagtc
     2941 atgggttaac gaggcggtag ccggcggagc ccggctgttg tgcggcggac agcggaaggg
     3001 cagttttttc gaacccacgg taataagctc ggcaaaaaat tccatgaaca tttgctgctg
     3061 cgaaattttc ggtcccgtgg tggtgctgga accttatgag gattttgaaa aagccgttga
     3121 agaggtcaat aacagccgct atggcttaca ggcaggggta tttaccgata gcgtggccga
     3181 aatggactat gcctttgata acctcgaagt cggcggggtc attattaatg atgtgccgac
     3241 ttttcgcacc gatcatatgc cgtatggcgg cataaaggat tccgggctgg gtagggaagg
     3301 cgtgaaatat gctattctcg atatgatgga gccgcggctg ctggtgaaat gatgatcatt
     3361 cgatcatcaa cgatgtcata gacaaagaac ccatcaccag tttgtcgttg aaataggtta
     3421 tagtttcgtt tgattgtttt aatcccaatg catataacag cggcaggtag tgttccggtg
     3481 ttggaacggc taattttagg ctatgataga atgttacatg aaattgtcat gaaaattatt
     3541 tatatttgtg aaaatacagc gaactatgaa aaagacgtat gtgctaataa tgttaatatt
     3601 ttcaatcgga tttacaaagg caaatccaat ttctccccct cctataatca gcgagttata
     3661 tattattaac gattctacat ggtttcttga aatagttttt ctgcaaggta atcctttcat
     3721 aaattatgca aatcttgatg gattcaggat aacaacttca atgggtacct cttatttaaa
     3781 aaacggtatc ccgatcatca atgatagtat tattgttata actcaggata gcttacaaag
     3841 tcccttacag atattcagga atggagattt tatattaatc aaagacattt caggactaca
     3901 attggatcag atatattttg gcaatatttc aggcacccag atatctgctc cgttattagg
     3961 acaatccata gtcaaccttg ctcatccatg ttgggacact tatcttaatc agccgggtac
     4021 agttaacaaa ttagtaaaag ataatcatcc tacaatcgga tatagtcctt ttcagccttt
     4081 ttctgctatt ggcattttca caggagttgt ttatgacagc gggcatcaac ctgttgccgg
     4141 gataaagata ggaaacagcc actacattga tcttgctccc acaaatattt gcgccaattt
     4201 ttattatttt actttaaccg actcgttggg aagttttaca tctcaggaat acagtggaag
     4261 tcgctatgtt gagcttttct ttcaatcttc atcggtatta gccgattcaa taataaatat
     4321 tgaacccgac tcaataaatt actatgaatt tactattgac acgcttttga ccggagttca
     4381 acaaaattat tttcaaacag gcattacctt attatgctat cccaaccctt caaccggcga
     4441 aacatttatt tcattttcaa tcccagcggg catacattat tcaaaagcta ttattaaaat
     4501 atatgattca aacagcgaga tggttcgtat tttacctgta aatactagta attcccaaaa
     4561 caattattcg ataaaatggg atggtttgtg ctacaataat gctacggcat ctggaactta
     4621 ttactgtaac ctcgaattag acggacgaaa atctgcttct aataaaataa ttattattaa
     4681 gtgaaattca taaaaatatc tatttttttt agcttaatcc ttttacatgg aattgtgtgt
     4741 tctcaaaacc tgcaatttta tcgtgaagac ttaacctttg aaataaagga tggatttttc
     4801 tatgttaacg gaatctataa tttttgtaat aacggagaca aagaaataca acaggttttg
     4861 ttttatccat ttcctgttga tagtctttat ggtcgggtag attccattaa tgcatttgac
     4921 gtaaatgcaa aatctcaaaa tattatttta aatataactg aaaaaggatt tttattcaac
     4981 atagggctta acccttatgg gataggtaaa tataaaatct cttacagaca aaaattattg
     5041 aaaaataagg ctgaatacat tttagtatca actcaaaaat ggggtgcccc atttgaaagt
     5101 tccaattaca agttaataag ctatgataac acgagaatta cagccacctc atatatcccg
     5161 gatagcataa agcaaataaa cgacaaggga atttattatt ggagtaaaaa agattttatg
     5221 ccagataaaa atatgatatt ttattttgac agatagatta tctcagtaaa gagatcgtgc
     5281 taatttccta taataaagaa atacccggtg gtgcgaagtt tagcaaaaca ttaaaaataa
     5341 tatcctcaat actacgaata atttttttag atagttcggg cctgccgggt gcagggtatg
     5401 ttcataattc tgacttatgc cttctcaatt ctgacatatg cattctcaat tctggcttct
     5461 gccttcttaa ttctggcttc tgccttctta attctgactt ttagtgtgct tccagccagt
     5521 tattcccaga gcttatatcc acatctaagg gaacttttat gatgatggcg ttttgcattt
     5581 gtgcctgtac caagggcagc atggtttcca gttccggctt atagacatca aacaccaact
     5641 catcgtgcac ctgcatgatc atgcggcttc ttgtctgcat ttctgtcatc tgtttgtata
     5701 tattgatcat cgcaattttg atgatatcgg cagcagagcc ttgaatcggg gcattgatgg
     5761 cattgcgctc ggcataaccc cttaccacgg cgttcgctga attgatgtcg cgcagataac
     5821 gcctgcggcc cagtatcgtt tccacataac cttttttccg ggcagatgaa atggtgtctt
     5881 ccatatattt ttttatcgca gggtatttta tgaaatattg ttctatgatc tctgcagcct
     5941 ctttacgctg gatattcagc cgttcagcta atccaaaagc cgaaatgccg tatatgatac
     6001 caaaatttac catcttggca ttacgccgca tttctttggt aacatcagcc aaagccacgt
     6061 tgtaaaccct cgaagccgtg gccgcatgaa tgtcgtgccc ctgcctgaaa tcttcaatca
     6121 tattagcttc ttcacttata gaggcaataa cacgcaattc gatctgtgaa tagtcggcgg
     6181 aaaccagcac gtagtcttcg ttacgcggga caaatgcctt gcggatttca cggcctcttt
     6241 ctgtccttac tggaatattc tgcaggttcg gattgttgga actcaaccgt cccgtggcag
     6301 ccaccgcctg gttatacgaa gtgtggatac gcccggtacg gggattgata agctcaggca
     6361 gcgcgtcaac atagg
//
LOCUS       DAOSFP010000102         6354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10756_length_6354_cov_3.770916, whole genome shotgun sequence.
ACCESSION   DAOSFP010000102 DAOSFP010000000
VERSION     DAOSFP010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10756_length_6354_cov_3.770916"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="PLI16_04240"
     CDS             complement(<1..1239)
                     /locus_tag="PLI16_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOH83798.1"
                     /translation="MNRELIIDSGPSEVLIALLENKKLVELHREKNSNTFSVGDIYLG
                     KVKRIVQGLNAAFIDIGYEKDAFLHYLDLGPQIHSLMKYTKGAVQGKALPLTSNEITF
                     DKNIEKSGKISQIIAVNQEIPVQIAKEPISNKGPRITSELAIAGRYLVLVPFSDRISI
                     SQKIKSSEDRNRLKRLVTSIKPKNFGIIIRTVAENKKVAELIADLEDLMQKWESLVAR
                     LGNAKAPKKILSEIDRSSVILRDILNETFNSIHVNDAAIFDELRNYIKTIAPEKADIV
                     KYYKGKDPIFEHFGIDKQIRNAFGKKVYLKNGAYLIIEHTEAMHVIDVNSGHRSNTDN
                     NQEANAMEVNMDAAGEIARQLRLRDMGGIISIDFIDLHVPTNRRMLFEKMRLEMDADH
                     AKHTVLPPSKFGVVQITRQRV"
     gene            complement(1471..1755)
                     /locus_tag="PLI16_04245"
     CDS             complement(1471..1755)
                     /locus_tag="PLI16_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH83799.1"
                     /translation="MTKAEIVAEIANKTGIEKLTVQTSVEAFMDAVKAAMQKGENVYL
                     RGFGSFIVKKRAQKTGRNISKNTTIVIPAHNIPAFKPAKVFTNEVKKSVK"
     gene            1918..2985
                     /gene="mutY"
                     /locus_tag="PLI16_04250"
     CDS             1918..2985
                     /gene="mutY"
                     /locus_tag="PLI16_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOH83800.1"
                     /translation="MKFSDNLISWYHQHKRDLPWRNTTDAYKIWVSEVILQQTRVEQG
                     QAYYERIIAKYPDIFSLANAREEDLLKLWQGLGYYTRARNMLKAAKEIVSNYGGEFPP
                     FYDKMIKIRGIGKYTAAAVLSFAYNMPYPVMDGNVKRVLSRYFCLTGDITSATANNEL
                     ENKLQKVFDKNRPADFNQAIMEFGALHCKIKNPECEKCVLKTGCKAFKQDKVAGLPEK
                     SKRTIVKTRYFYYLVPWQTVRGQKYTFLQKRETQDIWRHLFQFPLIETAKKLSWDRLA
                     VSAEFKDFLKNDCYEVLAVSDVYSHKLSHQTICAVFVQLQVFNGLHGEKINKVSAKNL
                     RKYPTSRLIEKYLEEHIFVDV"
     gene            3029..3382
                     /locus_tag="PLI16_04255"
     CDS             3029..3382
                     /locus_tag="PLI16_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83801.1"
                     /translation="MNSCKVLLFFVFLTLIACQSVREPQSGGQANSPSSVLSTGRVSH
                     QYRATGCLTVIIVDKPGESLVLIPYKPLAAQFDKDGLVLKFDYRLLKMPNPKGCEVGI
                     PAELSNVAIKQVPTK"
     gene            complement(3609..4157)
                     /locus_tag="PLI16_04260"
     CDS             complement(3609..4157)
                     /locus_tag="PLI16_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH83802.1"
                     /translation="MKHLSRLVLKLFGWRPVYQLPEGISNCILLVAPHTSFWDFPIGR
                     FSASAFKLHVRFLIKKEYFRFPLKHFLMWIGGIPVDREHGSNVAKHIVDVFKSAKNLC
                     LVITPEGTRKYTERWKKGYYFIATAAQVPIVLCYVDYKEKVCCVSDVVIYPSGNYEED
                     FKIIEKFYKGRVAKYPEKFNLS"
     gene            4230..6017
                     /gene="lepA"
                     /locus_tag="PLI16_04265"
     CDS             4230..6017
                     /gene="lepA"
                     /locus_tag="PLI16_04265"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOH83803.1"
                     /translation="MKNLRNFCIIAHIDHGKSTLADRMLEITGTVSARDFQNQVLDNM
                     DLERERGITIKSHAIQMTYEFEGQQYFMNLIDTPGHVDVAYEVSRSIAACEGALLVVD
                     ATQGIQAQTISNLYQALDHELEIIPVLNKMDMESAMPEEVKDQIVDLIGCRREEIIEA
                     SARTGKGITEILEAIVKRIPCPKGDPEKPLQALIFDSVFNSYRGIIAYFRIFNGELRN
                     NDFVKFFATDHEYHADEIGILKMDFLPREVMRAGEVGYIISGIKTAKEVKVGDTITHV
                     ERPCDVAVSGFQDVKPMVYAGVYPVDADDYEELRTSLEKLQLNDASLHFEPESSAALG
                     FGFRCGFLGLLHMEIVQERLFREFDMDVITTVPNVSFKVFTTKGEEVDVHNPSGLPPV
                     TTIEHIEEPYILAQIISKSEYTGQLMTLCIEKRGIMKNQVYLTSDRVELTFEMPLSEI
                     VFDFYDKLKSISRGYASFDYHIIGNRPATLVRLDILLNGEMVDALSTLIHRDYAYDFG
                     RKMCEKLQDLIPRQQYEVAIQAAIGAKIIARETIRPVRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGNIEVPQSAFLAVLKLG"
     gene            6094..>6354
                     /locus_tag="PLI16_04270"
     CDS             6094..>6354
                     /locus_tag="PLI16_04270"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOH83804.1"
                     /translation="MSGHNKWSTIKRKKGALDSKRSKIFSRIIKEINIAVKEGGPDPD
                     GNPKLRLAIANAKGANMPKDNVQRAINKASDKDASTYAEVTYE"
BASE COUNT         1777 a         1291 c         1439 g         1847 t
ORIGIN      
        1 tacgcgttgc cgggtaatct gtaccacccc gaatttgctg ggcggcagta ccgtatgttt
       61 ggcgtggtcg gcatccattt cgaggcgcat tttttcaaag agcattctgc ggttggtggg
      121 aacatgcagg tcgataaaat caatggaaat gataccgccc atatcgcgca gcctaagctg
      181 acgggcaatt tctcccgctg catccatatt gacttccatg gcgttggctt cctggttgtt
      241 gtcggtattg ctgcgatggc cgctgtttac gtcaatcaca tgcatggcct cggtgtgctc
      301 aatgatgagg taggcgccgt ttttcaggta cacttttttg ccgaaagcgt tacgtatctg
      361 tttgtcgatg ccgaaatgtt caaaaatggg gtctttgccc ttgtaatatt ttacgatgtc
      421 tgccttttcg ggggcaatgg ttttgatgta gttgcggagt tcgtcgaaga ttgccgcatc
      481 attaacatgt atgctgttga aagtctcgtt caggatgtcg cgcaagatta ccgaactccg
      541 gtctatttcg ctcaaaatct tttttggagc cttggcattg cccaaacgtg ctaccagcga
      601 ttcccatttc tgcatcaggt cttccaggtc ggctatcagt tcggcaactt ttttgttttc
      661 ggctaccgtg cggataatga ttccgaaatt ttttggtttt atgctggtta ccagtcgttt
      721 gagacggttg cggtcttcgc tgctttttat tttctgcgag atggaaatgc ggtcggaaaa
      781 tggaaccagt accaggtatc tcccggcaat agccaactcg gaagtgatgc ggggaccttt
      841 gtttgagatg ggttctttgg caatctgtac cggaatttcc tgattcacgg caatgatctg
      901 cgaaattttt ccggattttt caatattttt atcaaacgta atttcattgc tggtcagagg
      961 caatgctttt ccctgcacgg cacctttggt gtatttcatc agggaatgta tctgcggacc
     1021 cagatcgagg taatgaagaa aagcatcttt ttcgtaacca atgtcaataa aagcagcatt
     1081 cagtccctgt actatccttt taaccttgcc aagataaatg tcgcccacag aaaaagtgtt
     1141 gctgtttttt tctctgtgaa gttcaacaag ttttttgttt tcaagtaatg caatcaatac
     1201 ttcggaagga cccgaatcga tgataagttc cctgttcaaa gacaataagt tttaatgctt
     1261 taaaagtgtg ttgtggaaat ttgtagtggt atgcggaaga aacaaaaaaa acacctgccc
     1321 taaatcatat ctgtttaagg cagtgtgttt tacgaaatag ttatttcttc ttatgtctgt
     1381 tctttctcaa gcgttttttg cgcttgtggg ttgacatttt gtgtcttttt ctttttttac
     1441 cgcttggcat atttttaaaa tttagtaata ttatttaaca ctctttttta cttcgttggt
     1501 aaagaccttt gccggtttga aagcggggat attatgagcc gggataacga tggtggtatt
     1561 ttttgaaata tttcttccgg ttttttgtgc gcgttttttt acaataaaac tgccaaagcc
     1621 tctgagataa acgttttcac ctttttgcat agctgctttt acagcgtcca tgaaggcttc
     1681 aacggaagtt tgaacggtta gtttttcgat tcctgttttg ttagcaattt ctgctacgat
     1741 ttctgctttt gtcatgatga aaagaatttt tttataagtt aaataataag gttttaatta
     1801 gttgtcctct atttttaaat cggggtgcaa atataatctt attttttcaa aaccaatttt
     1861 ttttctcaac attatttttg ggaaaatgac ttattttgca ttaataatta acgggttgtg
     1921 aaattttcgg ataatttgat aagctggtat catcagcata aaagagattt gccctggcgt
     1981 aataccactg atgcttataa gatatgggtg tcggaggtta tacttcaaca aacgcgtgtt
     2041 gaacagggcc aggcatatta tgaacggata attgccaaat atcccgacat tttcagtctg
     2101 gcaaatgcca gggaggagga tttgctgaaa ttgtggcaag ggctgggtta ttatacacgg
     2161 gcacgcaata tgttgaaggc cgcaaaagaa attgtgtcta attacggcgg cgaatttccc
     2221 ccattttatg ataagatgat aaaaatcaga ggaataggaa aatataccgc ggcagcagtt
     2281 ttgtcatttg catacaatat gccctatccc gtgatggatg gcaatgtgaa acgggtattg
     2341 tcgcgttatt tttgcctgac cggcgatatt acttctgcaa ctgccaataa tgagctggaa
     2401 aacaaactgc agaaggtgtt cgacaaaaac aggcctgcgg atttcaacca ggccatcatg
     2461 gaatttgggg ccttgcattg caaaattaaa aatcccgaat gcgaaaagtg cgtgctgaaa
     2521 accggatgca aagcctttaa acaggataag gttgccggat tgcctgaaaa atcaaaaagg
     2581 acaatcgtaa aaaccagata cttttattac ctggtgccat ggcaaacagt acgcggccaa
     2641 aaatatactt ttctgcaaaa gcgcgaaaca caggatatct ggagacactt gttccagttt
     2701 ccgctcatcg aaactgcgaa aaagttatca tgggatcgtc ttgccgtatc tgcggaattt
     2761 aaagattttc tgaaaaatga ctgttatgag gtgctggctg tttctgatgt ttattcacac
     2821 aaactgagcc atcagaccat ctgtgctgta tttgtacaat tacaggtgtt caacggttta
     2881 catggtgaaa aaataaataa agtttcagcc aaaaatctga gaaaatatcc cacctcgagg
     2941 cttattgaaa aatacctgga ggaacatatt ttcgtggacg tatagaaaag cactactttt
     3001 actgagaact acatataagt ttttcaaaat gaatagttgt aaagtcctgt tgttttttgt
     3061 ttttctgact ttaatagctt gtcagagcgt cagagagccg cagtcaggag gtcaggccaa
     3121 ctccccttct tctgtcctgt caaccgggag ggtctcgcac caatacaggg ccaccggctg
     3181 tttaaccgta ataattgttg ataaacccgg cgaatcgttg gtgctgattc cttataaacc
     3241 attggctgcg caattcgata aggacggcct ggtgttgaaa tttgactacc ggcttttgaa
     3301 aatgcccaac cccaaggggt gcgaagtggg gattccggct gagttgagta atgttgccat
     3361 aaaacaggtg ccgacaaagt aaggctgtgg tgccgttaca ttgccaaaaa ggttttgcaa
     3421 ggagtcgtga ttccactccg aaataaattt tgtaaaaatc aaccttagcc cggccatgat
     3481 ggtttgttga tcttcaatga attccctttt gtgtcaaagc aaaaagtcgt tgaaaattaa
     3541 ttaattgtac tttttattgc ggactcaatc gtgaatttcc aaaagggtag gtgtgccaat
     3601 taattacatt atgagagatt gaatttttcg gggtatttgg ctactctgcc tttgtaaaac
     3661 ttttcgatga ttttaaaatc ttcttcatag ttgccgctcg gataaatgac cacatcggac
     3721 acacagcaca ctttttcctt gtagtccaca tagcacagca caatgggaac ctgggcggct
     3781 gtggcaataa aataatagcc ctttttccag cgttcggtgt atttgcgcgt tccttcgggc
     3841 gtgatgacca ggcaaagatt tttggcgctt ttaaaaacat caactatatg tttggctacg
     3901 ttgctcccgt gttcacgatc aacgggaatt ccgcctatcc acatcagaaa gtgttttaac
     3961 gggaaccgga agtattcttt tttaatcagg aatcttacat gaagtttaaa ggccgatgcc
     4021 gaaaaccgtc caatgggaaa atcccagaaa ctggtatggg gcgccaccag caagatgcag
     4081 ttgctaatgc cttcaggcag ttgatatacc ggtcgccagc cgaaaagttt taataccagg
     4141 cgtgataaat gtttcatgtt cttgccgcac aaaaattagg ggctgcaaaa ataataataa
     4201 aaaagcaaaa aagtagtaat tttgtgccga tgaaaaacct tagaaacttt tgtatcattg
     4261 cccatatcga ccatggcaag agcacactgg ccgaccggat gctcgaaatc acaggcactg
     4321 tctcggcccg cgattttcaa aatcaggtac tcgacaatat ggacctggaa cgcgaaaggg
     4381 gtatcacgat aaaaagtcat gccattcaaa tgacctatga atttgaagga cagcagtatt
     4441 ttatgaacct gattgataca cccgggcatg tggatgttgc ctatgaagtt tcccgttcga
     4501 tagctgcctg cgaaggcgcc ctgctggtgg tagatgccac acaggggata caggcacaaa
     4561 ccatctcaaa tctttatcag gcgctggatc atgaattaga gattatcccg gtgctgaaca
     4621 aaatggacat ggaaagtgct atgcccgaag aagttaaaga ccagatcgtg gaccttatcg
     4681 gctgtcgccg cgaagaaatc attgaagcca gcgcgagaac cggcaaggga ataactgaaa
     4741 tcctggaggc catcgtaaaa cgaataccct gcccgaaagg tgaccccgaa aaacctttac
     4801 aagccctgat atttgattcg gtgtttaatt cctaccgcgg cattattgcc tatttcagga
     4861 tttttaacgg cgaactcagg aacaacgact ttgtgaagtt ttttgccacc gaccacgaat
     4921 atcacgccga cgaaataggc attctcaaaa tggattttct cccgcgcgag gtgatgcggg
     4981 ccggcgaggt aggctatatt atctcgggaa taaaaacagc taaagaggta aaagttggcg
     5041 ataccattac tcatgttgaa agaccttgtg atgtggcggt ttccggattt caggatgtga
     5101 agcccatggt gtatgccgga gtatatccgg tagatgcaga tgattacgaa gaattacgca
     5161 cctcgctgga aaaactccag ttgaacgatg cctccctgca ttttgaaccc gaatcttctg
     5221 ctgcactggg atttggcttc cgctgcggct ttctgggcct gttgcacatg gaaattgtcc
     5281 aggaacgtct gttccgtgag ttcgacatgg atgtcatcac cacggtgccc aacgtttcct
     5341 ttaaagtctt taccaccaaa ggggaggagg ttgatgttca caacccttcg ggtttgccgc
     5401 cggtaaccac cattgaacac atagaggaac catacatatt agcgcaaatc atctccaaat
     5461 cggaatatac cggacaactg atgaccttgt gtattgaaaa gcgcggtatc atgaaaaacc
     5521 aggtttacct taccagcgac agggtggagc tgacttttga aatgcctctg agtgaaattg
     5581 tgttcgactt ttacgataaa ctgaaaagta tttcgagggg ctatgcctct ttcgattatc
     5641 atataattgg caacaggccg gcaaccttgg tccgccttga tattttactc aacggcgaaa
     5701 tggtggatgc gctgtccacg ctcattcatc gtgattatgc ctatgatttt ggccggaaaa
     5761 tgtgtgaaaa acttcaggat ctcataccgc gccagcagta cgaagtggcc atacaagccg
     5821 ccattggagc caaaattatt gcccgcgaaa ctatcaggcc ggtgcgcaag gatgttaccg
     5881 ccaaatgtta tggcggcgat atcacccgta aacgaaagtt gctcgaaaaa cagaaaaaag
     5941 ggaaaaaacg catgaggcaa ataggcaata tcgaagtgcc gcagtcggct tttctggcgg
     6001 ttttgaagct gggatagtta tttatttttt taagacagta atattttgta aatttgcact
     6061 ttgaaactca aacaaaaaac aaaaaattaa tatatgtcag gacataataa gtggtcaact
     6121 attaaaagga aaaaaggagc cctcgactca aaaagatcga aaattttttc cagaattatt
     6181 aaagaaatca acattgccgt aaaagaaggc ggccccgatc ccgacggcaa tcccaaactc
     6241 cgtctggcta ttgccaatgc aaaaggcgct aatatgccta aagataatgt gcagcgggct
     6301 attaacaaag cctctgataa agacgcatct acctatgctg aagttactta cgaa
//
LOCUS       DAOSFP010000103         6339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10793_length_6339_cov_4.078771, whole genome shotgun sequence.
ACCESSION   DAOSFP010000103 DAOSFP010000000
VERSION     DAOSFP010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10793_length_6339_cov_4.078771"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLI16_04275"
     CDS             complement(<1..352)
                     /locus_tag="PLI16_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83805.1"
                     /translation="MKTLRHFFLLFIMLSLLLSCNNKNNVADTPSESEQGYMLPRVLI
                     VTTGSNLGNGVLAEGVNIAMQTFNRLGAFVTLKSRDILLDPQELNRYNIMILPTAAGY
                     HDADRRYSLTFMADE"
     gene            complement(352..3600)
                     /locus_tag="PLI16_04280"
     CDS             complement(352..3600)
                     /locus_tag="PLI16_04280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH83806.1"
                     /translation="MKAKYLIILFLVMLLAFVVYQMLKIEDARTKKESLIQEEETELP
                     VELLSKEELVKLRHKVNYYAIANGNYFKIRQPRVVDGSRKSVWKDIYLKGVNIGTAMP
                     GYFPVEFSLSFDDYLQWFRMIGKMNSNVIRVYTILPPEFYDALAYYNLNNQNRCLYIL
                     QGIWTEEPLDRNYLNAGFMKSYQKEIRKAVEVINGKAYLKPKAGHASGVYSSDVSKYV
                     IGYLLGREWEPKSVMFTNAVNASVTKYPGLFVSINEGSAMEVFLAKMMDYLVHYETQT
                     FLKQHPVSFVNWLPLDPMYHNYEFIDNDKIREYDNDLVSIDFTKFQSSELFVPDIFAS
                     YHVYPYYPDYIFMEEKYRTTKNNRGQNDNFLGYLKDLKSRNEGIPLLIAEYGLPSSRG
                     NSHFSSQGFHQGGHSELAQAHLSSILTTDIHESACAGGLYFEWTDEWYKHNWLVMDFQ
                     QPAERRKLWHNMENPEQNYGILAVESRTKTVDGKLNDWKEIPAGNKEFIVADADAAYF
                     YLTLRLPTFNLNQNNLLIAFDTYDKKKGNHKLPGIEKEISNGIEFLLKIESVDNAKIL
                     VDDQYLLYTDVVKDIIPVYASKYNNNGIYAEQLLLANRGKETIMGEKIDSVCFNQGTL
                     HHGNSSDPRYSNADWYWDNQTKTLEIRLPWLLLNVSDPSSRSVLDDVPGTEAIESSVT
                     PGFKIFYYVTDKKGNTTAKTNISRPYKYTWATWEQPSYTTRLKPIYDTLKTLFAHLDV
                     IADTTPKTKDPATKFSICPYYGNRAGAVSITFDDADYSQYEYALPTLGKYNLKANFGL
                     VDSWLHETPSLIAEEGSFAIKRLGIKQVHDIIYEGHEISFHGNEHKKYAGMSAAQIIK
                     DFQESKKSLEAKLGINISVIHYPYSNSPDIVVNAAKNAGFLFGRTGEATPGKNANNDF
                     MKLNSVVFLNSENPSITILDSILANNKNSWTVLLYHHIFPKISKEYKLYESHNVTNNY
                     TVTPADFVKQIRLVRNSGYWIAPISAIGKYSKQRNSAVIKTSAHENAIFLRVDCPLNP
                     EIYNHPLTIRLETKNKKFRITNCAADGIYNARSNVLFFNIYPNQDVTIEILD"
     gene            complement(3600..5144)
                     /locus_tag="PLI16_04285"
     CDS             complement(3600..5144)
                     /locus_tag="PLI16_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83807.1"
                     /translation="MRFNKLKTGIILLLLGNFFSLYGQDATDSTSVSYLINKGQEACA
                     LGNFELSNQYYTKALLIDTANYDAKFGIANNHYRLKQYDKALEGYERLLHQYPDNTDI
                     LNGMARCYTKQESYPKAAACLKRSLAVNNQDADVYADMAFIHIVNNRLDSATLAYKKM
                     LEIDSTLAEAWAGIGKMYYWQDKPSSALKFYKKALSLDPGNQDYITKYTNVKSELASS
                     VSATLMYITEEEPSAIKDTPAYKISAFVQKYAFNKRLTDHFSLTVSTLWDYSQRDNLY
                     LSDVRRWFDNTLINPLFILKSHRISVYAGASINDSRITSFGGTWSFAYSIKKFKIRNY
                     LTTAYDYFYYWNKVGHNFAENTLILSYRGISLGGMYRYSVIRDNSVFVNESRDTIEKR
                     NNTNTRFKIELKYELKEFLKNPNITFGVNLYDMDYKYVSPLYYSPSNRTILGAMISEF
                     YSYKGFYNYFEFMYGRDNYNTNQYNGTLELGYEKNKTSFSLGGSYFYNKYYQSMNIRF
                     SVKQSF"
     gene            complement(5147..>6339)
                     /locus_tag="PLI16_04290"
     CDS             complement(5147..>6339)
                     /locus_tag="PLI16_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83808.1"
                     /translation="DRTLQTMLNSFSFTETGFELQGGIKTGTIKKVYRDSSNKILLID
                     KENGGKADSVNAGLNISSKKYICTIDADSILDDQALKMVLQPMIENPHVFVTGGFLAA
                     SNGLVISEGKISTRKMPRNIWVLWQIVEYMKSFMIARLSLSKMNMLLIMSGAFSLYRK
                     EDLLNIGGLLTGFNDHPYIVKTAGLRSKTVCEDMEVVIRLWRYYRENKIKGKVTFLPY
                     PVCWTEVPDNHKNLFKQRARWHQGLGESLKLHRKIMFEPKYGATGLLAMPYYFIFEFL
                     APFMKIFSLVFLIVLSLVKDIDAQWILLLLISVLLITTIIMSVITVVLETKSKNHLSI
                     DRKTLRYNTFGDWLLLLVTSIIGSVSYEFFKMFAQLKGIAYIMQKKSNWNKFERKGIK
                     TFEN"
BASE COUNT         1683 a         1306 c         1337 g         2013 t
ORIGIN      
        1 cttcatctgc cataaaagtc aatgaatacc tgcggtcggc atcatggtaa ccggcagcgg
       61 tggggagtat cattatattg taacggttca gctcctgcgg atcaagtaaa atatccctgg
      121 attttagcgt aacaaaggct ccaagcctgt tgaaggtctg catggcaata ttaacgcctt
      181 cagcaagcac cccattcccc agatttgatc ccgtggtaac aatcaatacc ctcggcaaca
      241 tatagccctg ctcgctttca gagggcgtat cagccacatt gtttttgttg ttgcacgata
      301 acaacaggct taacattata aatagcaaga aaaaatgacg tagtgttttc atcagtctaa
      361 tatttcaatg gttacatcct gattaggata aatgttgaaa aataaaacat tgctcctggc
      421 attatatatt ccatcggctg cacaattggt gatcctgaat tttttatttt tcgtctccag
      481 cctgatggta agcgggtggt tatatatttc aggattcagc gggcaatcca cacgaagaaa
      541 aatggcattt tcgtgtgccg aggtttttat aacggcgctg ttccgttgtt tactatattt
      601 accgatagcc gaaatggggg caatccagta gccggagttt cttaccaggc ggatttgttt
      661 tacaaaatcg gcaggggtaa cggtgtagtt attggtaaca ttatgcgatt cgtacagctt
      721 atactccttt gaaatctttg ggaaaatatg atggtaaagc aacaccgtcc acgaattttt
      781 attgttggcc aaaattgaat ccagtatggt aattgaaggg ttttccgagt ttaggaatac
      841 cacgctgttg agtttcataa agtcattatt ggcatttttt cctggtgtgg cctcgcctgt
      901 cctgccgaac agaaatcccg cgttttttgc cgcgttgacc actatgtcag gcgaatttga
      961 atatgggtaa tgtataaccg aaatgttgat tcctaatttg gcctcaaggc ttttcttact
     1021 ttcttgaaaa tcttttatta tctgggctgc agacatgccc gcgtatttct tgtgttcgtt
     1081 gccatgaaaa gaaatttcgt gaccttcata aatgatgtca tgtacctgtt ttatgccaag
     1141 tctttttatg gcaaagcttc cttcttcagc aatcagtgag ggcgtttcgt gcagccacga
     1201 atctaccagc ccgaagtttg ccttcaggtt atattttcct aatgttggca gcgcgtattc
     1261 gtattggctg taatccgcat catcaaaggt aatggatacg gcgccggccc ggttgccgta
     1321 gtaagggcaa atggaaaatt ttgtggcggg gtctttggtt ttaggcgttg tgtcggcaat
     1381 cacatccagg tgggcaaaaa gtgtttttag cgtgtcgtaa atgggcttta accgtgttgt
     1441 ataggatggc tgttcccagg ttgcccaggt gtatttgtac ggcctgctga tgttggtttt
     1501 ggcagttgtg tttccttttt tgtcggttac gtaataaaaa attttaaagc ccggtgtgac
     1561 agaactttca atagcttcgg tacccggcac atcgtcaagg accgaccggg aggaaggatc
     1621 gctgacatta agcagcagcc atggcaaacg gatttcaaga gtttttgtct ggttgtccca
     1681 ataccagtcg gcattggaat acctggggtc actgctattg ccatgatgca atgtaccctg
     1741 attaaaacag acagaatcaa ttttttcgcc catgatggtt tcttttcctc tgttggctaa
     1801 cagcaattgt tcagcataaa taccgttatt gttgtatttt gaagcgtaaa caggaatgat
     1861 gtctttcaca acatcagtat atagcaaata ttgatcgtca accaatattt ttgcattgtc
     1921 aacggattca atttttagga gaaattcgat tccattgctt atttctttct caattcctgg
     1981 caatttgtga ttcccctttt ttttgtcata ggtgtcaaaa gcaatcagga ggttgttttg
     2041 gttcagattg aatgtgggca ggcgcaatgt aaggtaaaag tatgcggcat cagcatcggc
     2101 aacaataaat tctttgtttc ccgcgggaat ttctttccag tcattcagtt tgccgtcaac
     2161 agtctttgtg cgtgattcca cggccagtat cccataattt tgttcgggat tttccatatt
     2221 gtgccacagt tttcttcttt cggcgggttg ctgaaaatcc atgacaagcc aattatgttt
     2281 gtaccattcg tcagtccatt caaagtagag gcctccggca catgccgact cgtggatgtc
     2341 tgtggtcagg attgatgata agtgggcttg tgccagttcg ctgtggccgc cctgatgaaa
     2401 gccctgcgag ctgaaatgac tgtttcccct gctggagggc aggccatatt ctgctataag
     2461 caagggtatg ccttcgttac ggcttttgag gtcttttaaa tatccaagaa aattatcatt
     2521 ttgtccccgg ttgttttttg tagtacggta tttttcttcc ataaagatat aatcaggata
     2581 atagggataa acatgatagg aggcaaaaat gtcgggcacg aacaactccg aggattggaa
     2641 ttttgtaaaa tcaatgctta ccagatcgtt gtcgtattcc ctgattttgt cattgtcgat
     2701 aaactcgtag ttatgataca tcggatcaag aggcagccag tttacaaagg aaaccgggtg
     2761 ttgtttcagg aaggtctgtg tttcgtagtg aaccaaataa tccatcattt ttgccaaaaa
     2821 tacttccatg gccgaacctt catttattga gacaaacaat cccgggtatt ttgttaccga
     2881 tgcattcact gcattggtaa acataaccga ttttggttcc cattccctcc ccagcagata
     2941 cccaataaca tatttggaaa catcggacga atataccccg ctggcatgtc cggctttagg
     3001 tttgagatat gctttgccat taatgacctc cacggctttc ctgatttctt tctgatatga
     3061 tttcatgaag cccgcattca ggtaatttct gtcaaggggc tcttcggtcc atattccctg
     3121 caggatataa agacaccggt tttggttgtt cagattataa taagcaaggg cgtcgtaaaa
     3181 ttcggggggc agtatggtat atacccgtat tacattcgag ttcattttcc ctatcatgcg
     3241 gaaccattga aggtaatcat caaaagataa tgaaaactca accgggaaat aaccgggcat
     3301 agcagtgcca atattcaccc ccttcagata aatgtctttc cagacacttt tacgactgcc
     3361 gtccaccacc cggggctgtc ttattttgaa gtaatttccg ttggctatag cataataatt
     3421 cactttgtga cgcaacttga ccaattcttc ttttgataac aattccaccg gaagttcagt
     3481 ttcttcttcc tgaatcagcg attccttttt tgttcgcgca tcttctattt tcagcatttg
     3541 atacacaaca aatgcaagca gcattaccag aaacagtatt atgagatact ttgctttcat
     3601 cagaacgatt gtttaaccga aaaccggata ttcatgcttt gataatactt attataaaaa
     3661 tagcttccgc ccagcgaaaa actggtttta tttttctcgt atcctaactc taatgtgcca
     3721 ttgtactgat tggtgttata gttgtcccgt ccatacatga attcaaaata attgtaaaat
     3781 cccttatagc tatagaattc tgatatcatg gcgcccagta tggttctgtt ggaaggggaa
     3841 taatacaatg gcgaaacata tttgtagtcc atgtcataaa ggtttacgcc gaaggttatg
     3901 ttgggatttt tcagaaattc cttcaattca tatttgagtt caattttaaa ccgggtgttg
     3961 gtattgttcc ttttttcaat ggtatcgcgg gattcgttta caaatacact gttgtccctg
     4021 atgacgctgt atcggtacat acctcccagg ctaatgcccc tgtaagacaa tattagtgtg
     4081 ttttcggcaa aattatgccc cactttattc cagtaataaa aataatcgta ggcggttgtc
     4141 aggtaatttc tgattttaaa ttttttaatt gaataagcaa aactccaggt gcctccgaac
     4201 gaagttatcc gggaatcatt gatgctggca cctgcatata cgctaatacg gtgagatttt
     4261 aggataaata agggattgat cagggtattg tcgaaccacc ggcggacatc ggacagatac
     4321 agattgtcgc gctgcgaata atcccagagc gtgctgacgg taagcgaaaa gtggtcggtc
     4381 aggcgtttgt tgaatgcata tttctggaca aatgccgata ttttgtatgc cggcgtatct
     4441 ttgatggccg aaggttcttc ttcggtaatg tacatcaggg tggcgcttac tgaggaggct
     4501 aattcacttt ttacattggt atacttagtg atataatcct gattcccggg atcaagtgat
     4561 aatgcctttt tataaaattt caaggcggag gaaggcttgt cttgccagta atacattttc
     4621 cctataccag cccatgcctc ggccagggtg ctgtctattt ccagcatttt tttatatgcc
     4681 aaagttgccg aatccaggcg gttgttgaca atatgaataa aagccatatc ggcgtaaaca
     4741 tcagcatcct gattgtttac cgccaacgac cgttttaaac aggccgccgc tttggggtag
     4801 gattcctgtt tggtataaca ccgtgccatt ccattcagga tgtctgtatt atcaggatat
     4861 tggtgcagca acctctcgta tccttcaagt gccttatcat attgtttcaa acggtaatga
     4921 ttgtttgcaa taccaaattt tgcatcataa ttggctgtgt ctatcagcaa tgcttttgtg
     4981 taatattgat tcgacaattc aaaattaccc aaggcacagg cctcctgccc tttgtttata
     5041 aggtaagaaa cggaggtgct atctgttgca tcttgtccat acaacgaaaa aaaattgcct
     5101 aacaacaata aaatgattcc tgtttttaat ttattaaaac gcatattcaa ttctcaaatg
     5161 ttttaatccc ttttcgttca aatttattcc agttgctttt tttctgcatg atgtaggcga
     5221 taccctttaa ttgtgcgaac atcttaaaaa attcataaga gacactgccg attatgctgg
     5281 taacaagcag caacaaccaa tcaccaaaag tgttataccg caatgtcttt ctgtcgatgc
     5341 tgagatggtt tttgctttta gtctctaata caacggtaat aacgctcata ataatggttg
     5401 taattaatag tacactgata agcagcaaaa gtatccattg ggcatcaata tctttcacca
     5461 gtgagagcac gatgagaaat acgaggctga atattttcat aaagggtgcc agaaattcaa
     5521 aaataaaata ataaggcatg gccagcaatc ctgtggctcc atatttgggt tcaaacataa
     5581 tcttccggtg aagcttcagt gattccccca atccctgatg ccatcgtgcc ctttgcttaa
     5641 aaaggttttt gtggttgtca ggaacttctg tccagcacac aggataagga agaaaggtta
     5701 ccttgccttt tattttgttc tcacggtaat aacgccacaa cctgatcacc acctccatgt
     5761 cttcgcagac ggtcttactt cgcaatccgg cggttttaac tatataggga tggtcgttga
     5821 atccggtaag caacccgccg atattcagca ggtcttcttt tcggtataaa gaaaaagcac
     5881 ccgacatgat cagcaacata ttcattttac tcaaactaag ccgtgcaatc atgaaagatt
     5941 tcatatattc aacaatctgc cacaataccc agatattgcg gggcattttt ctggtggata
     6001 ttttgccttc tgaaatgacc aggccgttgg atgcggccag aaaacctcct gtaacaaaga
     6061 catggggatt ttctatcatg ggctgaagca ccattttcag tgcctgatcg tcaaggatgg
     6121 aatccgcatc aatggtgcaa atgtattttt ttgaggatat gtttagcccg gcattaacgg
     6181 agtcggcctt gcctccgttt tccttgtcaa tgagtaaaat tttgttgctg ctgtcacgat
     6241 aaactttttt gatggtcccg gtttttatgc cgccctgtaa ttcaaacccg gtttctgtaa
     6301 aactaaatga attcagcatc gtttgcagcg tcctgtctt
//
LOCUS       DAOSFP010000104         6328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10817_length_6328_cov_4.540411, whole genome shotgun sequence.
ACCESSION   DAOSFP010000104 DAOSFP010000000
VERSION     DAOSFP010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10817_length_6328_cov_4.540411"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..813)
                     /locus_tag="PLI16_04295"
     CDS             complement(415..813)
                     /locus_tag="PLI16_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOH83809.1"
                     /translation="MKKIFILIWFCVLAVPLFSQDPAEDRSRYQYFDTINFPDGIKSI
                     TVYIKPDCGRCESVTSVLDDAVIPYTPVIINNDSVNRILEKKFAEAVKTTGIGWGMKY
                     PVLEINGTLYYVIANHWYFVNQLKDFYCKE"
     gene            1061..4222
                     /locus_tag="PLI16_04300"
     CDS             1061..4222
                     /locus_tag="PLI16_04300"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH83810.1"
                     /translation="MKKFFLFFLIILSTNYFLKGQVVINEYSCSNVSSFMDNFGEYED
                     WLEIYNPMAITVSLDGWYLSDKKTNPTKWRITSATIPSHGFLRIWASGRDIKTGTLHA
                     NFKLTQCLPEAIVIADASGDIVDSVTLKPTLEGHSRGRTTNGAATWGVFLTPTPNAGN
                     TNPYAEYATRPVMSVSAGFYTAAQNVAITSPDPGVTIRYTTNGSTPTAASAAYTSPVP
                     ITATTVLRAKAFSSSPNVPASFVESNTYFINSSHTVEVVSIFGDAVMALMNGTQSEPY
                     TGVEYFDHNGQFVTESYGNTNKHGNDSWAYSQRGIDFVSHDQYGYNYALLVQIFNSKP
                     RTEFQHIIFKAAANDNYPFSTAGSAHIRDSYVHTLSQKAHLHMDERTWAPCIMYVNGQ
                     YWGMYDTREKVDDKDFMEYYHDTKEDSLQMLKTWGSTWSEYGGAQAQTDWDNLRNFIV
                     TNNMADPANYHYVDSLYSIKSLADYVILNSFAVCSDWLNWNTIWWRGLNVEADKKKWR
                     YCLWDEDATFNHYINYTGIPNTNINADPCDPNSLNNPGGQGHIPVLNALLQNQDFYQY
                     YIMRYFDLLNGPLTCNRMTTLLDSMINVIQPEMQQHINRWGGTYSEWWTNYQTLRTFI
                     QDRCNMVVQLFDNCWPVTGPFRLVVDVDPPGAGTIELNSLTLTNFIWSGQYPGNMNIL
                     MKAMPSSNYCFDHWEMHHHTPVPGINDTAISFLFTTGDTVIAHFSGSTAPTAVATPST
                     ICVGDSSALQVSSGSSFLWSPATGLSCTTCANPIASPDVTTTYTVTVSGACGSGTASV
                     TVTVEQYGVSNPTVSATYTTLCLGDTTLLSCSNANTYSWSPAGSLSCTDCPNPLAVPT
                     STTTYTVSVTGYCSYGSASITIAIAPPPVLTHSGDITICPGSSTQLIASGGTTYTWSP
                     ADGLSCTNCANPLASPDMTTTYTVLAANGPGAGCQDSGALTVLVTGVCPDLYVPTGFS
                     PNGDHNNDIYLIFGDLEAMELVIYDRWGHEIFKSNDQSVGWDGTFPYNGKDAPTGVYA
                     YKLTVTDRKGEIIKKAGNITLVR"
     gene            4219..5256
                     /locus_tag="PLI16_04305"
     CDS             4219..5256
                     /locus_tag="PLI16_04305"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOH83811.1"
                     /translation="MRKKHFLIAVFILTGVSMLKAQDIHFSQISQTPLLLNPASTGAY
                     DGFYRGIINFKNQWVEMGKPYQTFMGSYDMPIYINKNRKSAAYLGAGALLFSDKAGNS
                     HLTTTHFGTAISCIVPVGSSQKLSAGILGGVTYSSIDLSAIQWPNQYNGQSYDPNLPS
                     YEVAKKSSYLFYDLAAGVLYHWQRPLNSFSGRQMASFDFGAALFHATKPKQRYFSLAN
                     ERKYPRVVFHADGRYDFEDTRVGIIPSALFMFQGASYELEAGMLVRILTNSVTNYTGY
                     TTESALSVGVHYRHKDAIIPQVYFEIHDFGVGLSYDINISSFSRASKLKGGLEISVKY
                     AKMRGALYRNR"
     gene            5370..>6328
                     /gene="purD"
                     /locus_tag="PLI16_04310"
     CDS             5370..>6328
                     /gene="purD"
                     /locus_tag="PLI16_04310"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOH83812.1"
                     /translation="MNILLLGSGGREHAFAWKLSQSPLVDKLYIAPGNAGTAFCGENI
                     DCSPLDFEKIALLVKEKKIGLVVVGPEDPLVQGIYDYFQAHAELKHIPLIGPSQKGAQ
                     LEGSKDFAKAFMQKYNIPTAAYRTFTRETLEEGLSFIDTLKPPYVLKADGLAAGKGVV
                     ILNNAGDARAELEQMLACSKFGKAGEKVVIEEFLKGIELSVFVLTDGHHYLVLPAAKD
                     YKKIGEGDTGPNTGGMGAVSGVPFADEVFMDKVHERIIKPTIKGLQQEKITYAGFIFI
                     GLMNVGGNPYVIEYNVRMGDPETESVLPRVKNDLVELLLATASG"
BASE COUNT         1773 a         1471 c         1406 g         1678 t
ORIGIN      
        1 gtttttattg tacttttaaa agattaaata tttttaaacg catcactact aaatttcaat
       61 ataaattaat tgattattgt aaaaataata atctgccgat ttcaagggcc catataaatc
      121 aacgtattgg accaataata attctttgcc atttttataa gcggtttcag taagattatg
      181 attggtgtta gtcgcgtcca aaatgctgtc ggtttcgctt ttaattttat gataattgtt
      241 cagattgctt ttaatgtcaa aaacaatttt atcaatttta gaaatagttt atttttcagt
      301 ttgagcaaaa attccctgaa ttaatgctaa gctgataaaa atgacagtta ctatgttttt
      361 catatttgtc aattcctctt ttaaattatt acagcaaccc tcagcgcgga aaagctattc
      421 cttacaataa aagtctttta gctgattgac gaaataccag tgattagcga taacataata
      481 taatgtcccg tttatttcca gcacaggata tttcattccc cagcctatac cggttgtttt
      541 gacggcttcg gcaaattttt tctctaaaat ccggtttacc gaatcattat taattattac
      601 cggagtataa ggaattactg cgtcgtcaag cacactggta accgattcac agcgtccgca
      661 atcaggttta atataaacag tgattgattt tataccatcc ggaaaattta tggtgtcaaa
      721 atattgataa cggctgcggt cttcggccgg atcctgcgaa aaaagaggaa cagctaacac
      781 acaaaaccaa atgagaataa aaattttttt cataatcata caaaattaac tgaaaaatca
      841 tcagaaaaat gttttccgaa aaggttcttt caatttttat accataatcg gtctgatggc
      901 gggcagatat cccttgaaaa actaaaaaaa gaagaaagag atttcctcgg ttttgcatta
      961 tctgaaacat tatgtataaa tacaaatctc cggaaaaatt tgtttgcaaa aaccctttaa
     1021 aatgcgtata ttcgtaatag tttttccaaa ctgcctgaga atgaaaaaat tttttctttt
     1081 tttcttaata attctgtcaa caaattactt tctcaaaggc caggttgtca tcaatgaata
     1141 ttcctgctct aacgtcagtt cgttcatgga taattttggt gaatatgaag actggttaga
     1201 gatatataac cctatggcta ttaccgtaag tttggatggc tggtatctga gcgataaaaa
     1261 aaccaacccc accaaatggc ggataacctc tgccaccata ccctcgcatg gttttctgcg
     1321 catctgggct tcgggcaggg atataaaaac cggcacccta catgccaatt ttaaacttac
     1381 gcaatgcctg cccgaagcga ttgtcattgc agatgcttcg ggagacattg ttgactcggt
     1441 aactttaaaa cccactctgg aaggacattc aagaggaagg accaccaacg gggcggccac
     1501 ctggggtgtg tttttgacac ccacgcccaa cgccggcaac accaacccgt atgccgaata
     1561 tgccactcgt cctgtgatga gtgtttcagc cggtttttat acggcagccc aaaatgtcgc
     1621 gattacctcg cccgaccccg gcgtaactat acggtacacg acaaatggaa gcacgccaac
     1681 agcggcatct gcggcgtaca caagcccggt ccccattacc gccaccacag tattacgggc
     1741 caaagctttc agctcctcac ctaatgtccc cgcgagtttt gtggagagca acacttattt
     1801 tatcaacagc agccatacgg tggaagtggt ttccatattc ggggatgccg tgatggctct
     1861 gatgaacggc acccagagcg aaccgtacac cggggtggaa tatttcgacc acaacggaca
     1921 atttgttacc gaaagttatg gcaacaccaa taaacatggc aacgattcct gggcttattc
     1981 gcagcggggt atagactttg tgagccacga ccagtacggc tataattatg ctttgttggt
     2041 acagatattc aattccaagc cgcgcaccga gttccaacac atcattttca aagcggccgc
     2101 caacgataat tacccgtttt ccacagcggg ctctgctcac atacgcgact cgtatgtaca
     2161 cactctttcg caaaaagccc atctacacat ggatgaaaga acctgggcgc cctgcattat
     2221 gtatgtaaac ggccaatact ggggcatgta cgatacccgc gaaaaagtgg atgataagga
     2281 ttttatggaa tattaccatg ataccaaaga agactcactc caaatgctga aaacctgggg
     2341 aagcacctgg agcgaatacg gcggggccca agcacagacg gactgggaca acctccgcaa
     2401 ctttatagtt accaacaaca tggccgatcc ggccaactat cattatgtgg acagtttgta
     2461 cagcataaaa agccttgccg attacgtgat attaaactcg tttgccgtat gttccgattg
     2521 gttgaactgg aacactatct ggtggcgcgg gcttaatgtg gaagccgaca aaaagaaatg
     2581 gcgttattgc ctgtgggacg aggatgccac gtttaatcat tacatcaact acacaggcat
     2641 acccaacacc aatatcaatg ccgacccatg cgaccctaat tcgctgaaca atcccggagg
     2701 acagggacat attcctgtgc tcaatgccct gctgcaaaac caggattttt atcagtacta
     2761 catcatgcgg tatttcgacc tgctgaacgg gccgcttacc tgcaaccgca tgacaaccct
     2821 gctcgacagc atgatcaatg tcattcagcc cgaaatgcaa cagcatatca accgatgggg
     2881 aggcacatac agcgagtggt ggacaaatta tcaaactttg agaaccttta tacaggaccg
     2941 ctgcaatatg gtggtacaac tgtttgacaa ctgctggcct gttacaggcc cctttcggct
     3001 ggtggtggat gtagatccgc ccggagccgg caccatagaa ctcaattcgc tgacattaac
     3061 caactttatc tggtcgggac aatacccggg caatatgaat atcctgatga aagccatgcc
     3121 atcttcaaat tattgttttg accactggga gatgcaccac cacacaccgg tacccggcat
     3181 aaacgacacc gcgatttcct ttctttttac cacgggcgat accgtgatag cacatttctc
     3241 gggcagcact gctcctactg ccgttgccac gccatcaaca atatgtgtcg gcgactcttc
     3301 ggcgctgcag gttagttcgg gaagttcgtt tttatggagt cccgcaacgg gcttaagttg
     3361 caccacctgt gccaacccta tagcctcgcc ggacgttaca actacctata cggtaaccgt
     3421 gtccggtgct tgcggaagtg gcaccgcgtc ggttaccgtt accgttgagc aatatggtgt
     3481 gtcgaaccca acggtatccg ctacttacac aactttgtgc ctcggcgata ccacgctgtt
     3541 aagttgcagc aacgccaata cctattcctg gagtccggcc ggcagtttga gctgtaccga
     3601 ctgtccaaac cctttggctg tacccaccag tacaaccact tataccgtat cggtaaccgg
     3661 atattgcagt tatggctcgg caagcattac catcgccatt gcaccgccgc cggtattaac
     3721 acatagcggc gatattacca tttgtcccgg aagcagcacc caattgattg cttcgggcgg
     3781 aacaacttat acctggtcgc ctgccgacgg gctgagctgc actaattgcg ccaatcccct
     3841 ggcttcaccg gatatgacaa ccacctatac agtgcttgct gctaacggac cgggagctgg
     3901 ttgccaggat tccggagccc tcaccgtatt ggtaacaggt gtgtgtcctg atttgtatgt
     3961 acccaccggt ttttctccaa atggtgacca caacaacgat atctatctga tttttggcga
     4021 tttggaagct atggagctgg tgatttacga ccggtgggga cacgaaattt ttaaaagcaa
     4081 cgatcagtcg gtgggatggg atggtacctt tccatataac ggcaaggatg cgccaacagg
     4141 ggtttacgca tataagctca cggtaacaga ccgcaagggc gagataataa aaaaggcagg
     4201 aaacataaca ctggtaagat gagaaaaaaa cattttctga tagcggtgtt tattcttaca
     4261 ggcgtgtcca tgctgaaggc acaagacatt catttttcac agatcagcca gactccgtta
     4321 ctattaaatc ctgcttccac cggggcttat gatggatttt accggggaat tatcaatttt
     4381 aaaaaccagt gggtggaaat gggtaagccc taccagacct tcatggggtc atatgatatg
     4441 cccatctata tcaacaaaaa tcgaaaaagt gcagcttatc tgggtgcagg ggctttattg
     4501 ttttccgata aagcaggcaa ttctcatttg accaccaccc attttggtac agctatatcc
     4561 tgtattgtac ctgtagggtc gagccagaag ttatccgcag gcatcctggg tggtgttact
     4621 tacagctcta tagatctttc agctatccaa tggcccaatc aatacaacgg acagagttac
     4681 gaccccaacc tgccatccta tgaggtggcg aaaaagtcat cctatttgtt ttacgacctg
     4741 gctgccggcg tgttgtatca ctggcaacgg cctttaaatt ccttcagtgg aaggcaaatg
     4801 gcgtcttttg atttcggggc agccttgttt catgccacca agccgaaaca aaggtatttt
     4861 agtcttgcca atgaaaggaa gtatccgcgc gttgtatttc atgccgacgg acgttatgat
     4921 tttgaggata ccagagtggg catcataccc tcggcccttt ttatgtttca gggggcgtct
     4981 tacgaattgg aagccggcat gcttgtaagg atacttacca acagcgttac caactatacc
     5041 ggatacacca ccgaatcggc cttatccgta ggggtgcatt accggcataa agacgctatc
     5101 attccgcagg tttattttga aatccacgat tttggcgtcg gactgtctta tgatatcaac
     5161 atttcttctt tttcacgtgc atcaaaacta aaaggaggcc tcgagatatc agttaaatat
     5221 gcaaaaatga ggggtgcttt gtaccgaaac aggtagccgg gataattgat ggtgatcctg
     5281 caggttaaat tcgtgaattt atcatgcagg tatcaatacc gcctcaagaa tttgttttaa
     5341 ttttgggcct gaatacagac taccattata tgaatatttt actcctggga tccggcggac
     5401 gcgaacatgc ttttgcctgg aagctttcac aaagtccatt agtggataag ctttacattg
     5461 cccccggaaa tgccggtacg gccttctgcg gcgaaaacat tgactgcagc ccgttggatt
     5521 tcgaaaaaat cgcgctgttg gtcaaagaaa aaaaaatcgg gctggtggtg gtagggcctg
     5581 aagatccttt ggtccagggt atctatgatt atttccaggc acacgctgaa ctaaaacaca
     5641 ttcccctgat agggccttcg caaaaaggtg cacaactcga aggaagcaag gacttcgcca
     5701 aggcgtttat gcaaaaatat aacatcccta ccgctgccta ccgtacattt accagggaaa
     5761 cactggaaga aggactttct tttattgaca ccttaaaacc tccttacgtt ttaaaggccg
     5821 acgggctggc tgccggaaaa ggcgtggtaa ttttaaacaa tgccggtgat gccagggcag
     5881 aactggaaca aatgctagcc tgttcaaaat ttggtaaggc cggcgaaaaa gtagttatcg
     5941 aggaatttct taaaggtatt gagctctctg tttttgtgct taccgacggc catcattacc
     6001 tggtgttgcc cgctgccaaa gattataaaa aaatcggcga gggcgatacc gggccgaaca
     6061 cgggcggtat gggggctgtt tcaggtgttc cctttgccga tgaagtgttt atggataagg
     6121 tgcatgaaag gattattaaa ccaactatca aggggctgca gcaggagaaa atcacgtatg
     6181 ccggttttat ttttatcggc ctgatgaatg tgggaggcaa tccatacgtg attgaataca
     6241 atgtgcgcat gggcgaccct gaaacagaaa gcgtgttgcc ccgcgtaaaa aacgacctgg
     6301 tggaactgtt gctggcaacg gcaagcgg
//
LOCUS       DAOSFP010000105         6320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10833_length_6320_cov_4.717638, whole genome shotgun sequence.
ACCESSION   DAOSFP010000105 DAOSFP010000000
VERSION     DAOSFP010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10833_length_6320_cov_4.717638"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1024
                     /gene="era"
                     /locus_tag="PLI16_04315"
     CDS             146..1024
                     /gene="era"
                     /locus_tag="PLI16_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOH83813.1"
                     /translation="MHKAGFVNIIGSPNVGKSTLMNALVGEKLSIITRKAQTTRHRIM
                     GIINGEDFQIVYSDTPGIIKPHYKLHEAMMTQVAAALSDADIILYMVEAGEKISEQTD
                     IIGQINKTDIPILLLINKIDTTDAAVVDSCEQIWAAQFKNAKVFRISALQKINIQQVF
                     DAVLALLPESPPYYSKDDLTDKPVRFFVSEIIREKVLLLYSKEVPYSVEVFIESFKEE
                     PDIVRIAATLYVARETQKAIILGHKGTGIKRLGIEARKDIEHFVGNKVFLDLTVKVNE
                     NWRDNAAMLQRFGYEL"
     gene            1114..2418
                     /gene="der"
                     /locus_tag="PLI16_04320"
     CDS             1114..2418
                     /gene="der"
                     /locus_tag="PLI16_04320"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOH83814.1"
                     /translation="MSNILAIVGRPNVGKSTFFNRLTGERNAIVDPTSGVTRDRHYGK
                     SDWNGISFSVIDTGGMVMGSEDIYEDEINKQVSLAIEESDAIAFMLDVMDGLTPLDED
                     MAVMLRKIKKPVFLLINKVDTHERIAAAAEFYSLGFERVFTISSVNGAGTGEFLDELM
                     KYFTVEEKQEEPDLPRIAVVGRPNAGKSSLVNVLLGTERNIVTPLAGTTRDSIYTRFN
                     SFGFDMLLVDTAGLRKKQKVTEDIEFYSTLRAIRAIENSDVCLLMIDAGKGIGAQDMH
                     IFSIIEKNHKGVVIVVNKWDLIEKETQTHLTFEAEIKEKIAPFTDVPVVFASALTKQR
                     IHKTLDTAISVYKNRTQKISTSKLNELLLPLVTMSPPPSVKGKFPKIKYIQQLPLHYP
                     AFAFFCSHSQYVKDTYKRFLENKLRTHFDFSGVPIEIFFREK"
     gene            2420..2794
                     /locus_tag="PLI16_04325"
     CDS             2420..2794
                     /locus_tag="PLI16_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOH83815.1"
                     /translation="MEEAKIRIDKWLWAVRIYKTRSLATEACRNGKVHIGDQAVKAAR
                     EVKPNDIIHIHQHQIIKTVKVIALLEKRVSAKLVAQFMEDLTPPSEYEKVEMIRAVSF
                     VYRPKGTGRPTKKERRDIEKFK"
     gene            2805..3236
                     /locus_tag="PLI16_04330"
     CDS             2805..3236
                     /locus_tag="PLI16_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOH83816.1"
                     /translation="MKRLTLLVLLLFTIAVQAQEVSEKNRYEIVAVLTHQQKCWNNGD
                     IDGYMDGYWKSDSLKFITKNGVTAGWEQTLSSYKQHYTSREMMGTLVFDEVSMLQMNT
                     VTVFVMGRWTLQRTNETVGGRFTQLVKKIGGKWKIIIDHTS"
     gene            3370..4344
                     /locus_tag="PLI16_04335"
     CDS             3370..4344
                     /locus_tag="PLI16_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH83817.1"
                     /translation="MTSLKEITRPIDINLAEFNSLYRETMHSNSALLDKVLGYILKTK
                     GKQIRPILVLLSAGLTGKINKSTYTAAILIELLHTATLIHDDVVDESDRRRGFFSVNA
                     LWKNKVAVLAGDYLLSKGLLVSLESKEYKYLHLGSDAIQNMSEGELLQIKKSRTMNID
                     EETYITVIRKKTASLFAACCSAGTASTTNDNDLIEKMKAFGENLGMAFQIKDDLLDFD
                     LTNKTGKPAHNDLKDRKFSLPLIYTLKNISFTEKRSLLGILKNHTQSKDKIEHLIQRI
                     REEKGFEYAEARMNEYKNMALQTLTDFPDSEYRKSLENLVTFITDRVY"
     gene            4399..6261
                     /locus_tag="PLI16_04340"
     CDS             4399..6261
                     /locus_tag="PLI16_04340"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOH83818.1"
                     /translation="MKKFLALMLCMNIFFAAFSQEKKTEYWKNNKKKGEGAVINGLKD
                     GKWIYWYEDGKKWCEGAYKNDKQTGQWIYWHVNGNKWEDINYENGLNTRYHSNGQKEY
                     EVNIVNGQRDGKWTEWYKNGKVKEEGAYVNGLKDGLWSNFYEDGAKQAQRYFSQDKFT
                     GKWTYWFPDGKLWKEISTENGPVTIYDSLGHKTSEWSVKNGKKDGRSLTWFTDGKTKS
                     ETTFVNDLREGVQTIYHPNGQKMLEGQFKNDEKSGKWLQFYENGKKWKELNYDNGLNI
                     AWYEDGTKQEEGYVKNGEKDGVWTKWYANGKMQEKINYKNGLREGPAQTWFDNGNKEM
                     EGFYIQDSLNCYARWWRNNGNKEMEGKIRGDLQDSVWIFYHENGAKGSTGNFARGKQQ
                     GAWVYYYETGEKWKDGYFTDGKKSGLWTTYYEDGKKQREGTFENDKENGLWTSWYRNG
                     QKQDMGTFNDGKMHLKWEGWFEKGNKKYIAEYNDGQRSGKYTLWWENGNMRTEQMYKN
                     DKKNGASSEWYENGKITESGYYTNDLKSGYWSFYDQYGVLFQEQGFSQGRPDGKFVEY
                     HQNGQKSSQGLYVTALREGTWYYWDKDGKLLYKVSFTKGKKVSEENAGKPEPKK"
BASE COUNT         2100 a         1237 c         1465 g         1518 t
ORIGIN      
        1 tttttcataa accctatttt aacatttttt aatagtacat aattttatgc tattcataat
       61 gccaaataaa ccgggtgaat gtacccggca atacacaaac atacacaatt cagtattatt
      121 ggttatcttt gcagatattt gtattatgca taaagcaggt tttgtaaata ttatcggtag
      181 tcccaatgtg ggaaaatcaa cactgatgaa tgcgctggta ggagagaaac tatccatcat
      241 cacgcgaaaa gcccaaacca cacggcaccg tattatgggc atcatcaatg gtgaggattt
      301 tcagattgtt tattccgaca cccccggtat tataaagcca cattataagt tgcatgaagc
      361 catgatgaca caggtggctg cggctctttc cgacgcagac attatattat atatggtaga
      421 agccggcgaa aaaatttctg aacaaacaga tataattggt cagattaata aaaccgatat
      481 accaatattg ttgttaatta ataaaataga tactacggat gccgcagttg ttgattcctg
      541 tgaacaaatt tgggctgcac aatttaaaaa tgcgaaagtg ttcagaatct cggctttaca
      601 aaaaataaat attcagcaag tttttgatgc agttttggct ttattgccgg aaagtcctcc
      661 ttattattca aaagacgacc tgaccgacaa acccgtacgt ttttttgttt cggaaatcat
      721 acgcgaaaaa gtgcttttgc tgtattcaaa agaagtgcct tattctgttg aagtatttat
      781 tgagtcgttt aaggaagagc cggatattgt ccgtattgca gctacattgt atgtggcaag
      841 agagactcaa aaagcaataa ttctgggaca taagggtacg ggaatcaaac gactgggtat
      901 tgaagcccga aaagatattg aacattttgt agggaataaa gtttttttgg accttacggt
      961 aaaagtaaat gaaaactggc gcgataatgc ggcgatgttg caacggtttg gatatgaact
     1021 ttagttggaa gttgcaagtt gaaagtggtt ccgattccga taactatcgg aagtgagaag
     1081 aagttatagt gattaggaaa aaaatattat aagatgtcga atatattagc tattgttggt
     1141 aggcctaatg tggggaaatc aacatttttc aaccgcctta ccggcgaacg caatgccatt
     1201 gtagacccca ccagcggtgt aacccgcgac aggcattatg gaaaatccga ctggaatggc
     1261 atttcttttt cggtgataga tacgggaggc atggtcatgg gctccgaaga catttacgaa
     1321 gacgagatca acaaacaggt cagtcttgcc attgaagaat ccgatgccat agcttttatg
     1381 cttgatgtca tggatggcct cacaccactt gatgaagaca tggccgtgat gttgcgcaaa
     1441 attaaaaaac cggtttttct gttgataaat aaggtggata cccatgaacg gatagccgct
     1501 gccgccgaat tttacagctt gggcttcgaa agggtgttta ctatttcatc ggtaaatggt
     1561 gccgggacag gggagtttct ggacgaactt atgaaatatt tcactgttga agaaaaacag
     1621 gaagagcctg acctgccaag gatagccgtt gttggccggc ccaatgcggg taagtcctcg
     1681 ctggtaaatg tactgctcgg aacggaacgc aatatcgtta cccccctggc cggaacaaca
     1741 cgcgacagta tctatacccg tttcaacagt ttcggctttg atatgctgct ggttgatacc
     1801 gccggattgc gcaaaaaaca aaaagttact gaagacatag agttttattc caccctgcgg
     1861 gccatacgcg ccatagaaaa cagcgacgta tgcctgctga tgatcgatgc cggaaaaggt
     1921 ataggcgcac aggatatgca tattttcagc attatcgaaa aaaaccacaa aggtgttgtt
     1981 atcgtggtca acaaatggga tctgattgag aaagaaaccc aaacccattt aacttttgaa
     2041 gccgaaataa aagaaaaaat tgctcctttc acggatgtgc cggtggtttt tgcttcggcc
     2101 ctgacaaaac aacgtattca caagacgctc gacaccgcca ttagcgttta taaaaaccgc
     2161 acccagaaaa tttccacatc aaaactcaac gaactgttgc tcccgttggt taccatgagc
     2221 ccccccccct ctgtgaaagg gaaatttccc aagataaaat acatacaaca gttgccgctt
     2281 cattatccgg cttttgcttt tttctgtagc catagccagt atgtaaagga tacctacaag
     2341 cgctttctgg aaaacaaact aagaacgcat ttcgacttca gcggggtgcc gatagagatt
     2401 tttttcaggg aaaaataata tggaagaggc caaaatccgc atcgacaaat ggctgtgggc
     2461 agtcaggata tacaaaaccc gcagtctggc caccgaggcc tgccgcaatg gaaaggtgca
     2521 tattggcgat caagccgtaa aggctgcacg tgaagtaaaa cccaacgata taatccacat
     2581 tcatcagcac caaataataa agactgtcaa agtaattgcc ctgctcgaaa aacgtgtttc
     2641 ggccaaactg gtagcacaat ttatggaaga cctcacaccc ccttccgaat acgaaaaggt
     2701 agaaatgata agggctgttt cttttgtgta ccgccccaaa ggcaccggga ggccgaccaa
     2761 aaaagaacgg agggatatag aaaaatttaa ataatccata agccatgaaa cgtttgacat
     2821 tattggtttt gttgcttttt accatcgctg ttcaggcaca agaagtcagc gaaaaaaacc
     2881 gttacgaaat tgttgcggtg cttacccatc agcaaaaatg ctggaacaat ggagatatcg
     2941 atgggtatat ggacggatac tggaaatcag attcgctgaa gtttatcaca aaaaacggtg
     3001 ttactgccgg ttgggaacag acactctctt cttacaaaca gcattacacc tcccgggaaa
     3061 tgatgggaac cctggtattt gacgaagtga gtatgttgca gatgaatacc gtcacggttt
     3121 ttgttatggg cagatggaca ctgcaaagga ccaatgaaac tgttggcggc cggtttaccc
     3181 aactcgtgaa aaaaatcggc ggaaagtgga aaatcatcat tgatcacacc agttgatacg
     3241 tagtgccacc caaacgtaca aacagttgcc aacgaagtct tttgacatga aaaactgccg
     3301 gtggaagaat attatttttt tatttataag gaactatttt cttacttttg aattcgagaa
     3361 tgacagcgga tgacctcact taaggaaata accagaccta tagatatcaa cctggccgaa
     3421 tttaacagcc tttaccggga gactatgcac agcaattcgg ctttgctgga taaggtgctg
     3481 ggttatatcc tgaaaaccaa aggaaagcaa atccggccta ttttagtttt gttatcagcc
     3541 gggttgacgg gcaaaataaa caaatctacc tataccgcag ccattttgat agaactattg
     3601 cacacagcca cactgataca cgacgatgtg gtggatgagt cggacaggcg gcgcggtttt
     3661 ttttctgtca acgccctctg gaaaaataaa gtggctgtgc tggccgggga ctacctgctc
     3721 tcgaagggac tgctggtttc tcttgaaagc aaggaataca aatacctgca tctgggctcc
     3781 gatgccatac aaaatatgag cgaaggcgaa ctgttgcaga taaaaaaatc acggacgatg
     3841 aatatcgatg aagagacata tatcaccgtt atccgtaaga aaacagcctc actttttgcc
     3901 gcctgttgct cggcaggaac cgcttcaacc accaacgata atgatcttat tgaaaaaatg
     3961 aaggccttcg gtgaaaatct tggtatggct tttcagataa aagatgacct gcttgacttt
     4021 gacctgacca acaaaaccgg gaagcccgca cataacgacc tcaaagaccg aaagttttcg
     4081 ctgccactga tctacaccct gaaaaacatc agttttacag aaaaacgcag ccttcttggc
     4141 atactgaaaa accataccca aagcaaagat aaaattgaac atctgattca gcgtatcagg
     4201 gaagaaaaag gttttgaata tgccgaagcc cgcatgaatg agtataaaaa tatggcgctt
     4261 caaaccctta cggattttcc cgattcggaa tacagaaaat cactggaaaa cctcgttact
     4321 tttatcaccg accgtgttta ttagatttgg caagatgcac ctgacacaaa atttgaaaat
     4381 aaaaaccact aaccgactat gaagaaattt ttggcattga tgctttgtat gaacattttc
     4441 tttgctgctt tttcgcagga gaagaaaaca gaatactgga aaaacaacaa aaagaaaggt
     4501 gaaggcgccg tgataaacgg cctgaaagat ggtaaatgga tttattggta tgaggatggc
     4561 aaaaaatggt gcgaaggtgc ctacaaaaat gacaagcaaa cagggcaatg gatttactgg
     4621 catgtgaatg gaaacaagtg ggaagacatc aattatgaaa acggattgaa caccaggtac
     4681 cactccaacg gacaaaagga atatgaagta aatattgtta acggccagcg cgacggcaaa
     4741 tggaccgagt ggtacaaaaa cggcaaagtt aaggaagaag gcgcttatgt aaatggcctg
     4801 aaagacggct tgtggagcaa tttttatgag gacggagcca aacaggctca acgttatttt
     4861 tcccaagaca agtttaccgg caaatggaca tactggtttc ctgacgggaa actctggaaa
     4921 gaaatatcta ccgaaaacgg tcctgtaacc atttacgatt ctcttggtca taaaacaagc
     4981 gaatggtccg tgaaaaacgg aaaaaaagat ggccgttcgc ttacctggtt tactgacgga
     5041 aaaacaaaaa gcgaaacaac ttttgtgaac gacctgcgtg aaggtgtgca aacgatctat
     5101 catccgaacg gccaaaaaat gctggaagga caatttaaaa acgacgaaaa atcgggcaaa
     5161 tggcttcagt tttatgagaa tggcaaaaaa tggaaagaac tgaattacga taacggcctg
     5221 aacatagcct ggtatgaaga cggaacaaaa caggaagaag ggtatgtcaa aaacggtgaa
     5281 aaggatggcg tatggaccaa atggtatgcc aacgggaaga tgcaggaaaa aatcaattat
     5341 aaaaacggcc tcagggaagg gccggcacaa acctggttcg acaacggcaa caaagaaatg
     5401 gagggttttt acatacagga ttcgctgaat tgttatgccc gctggtggcg aaacaacggc
     5461 aacaaagaga tggaaggcaa aataagaggt gacctgcagg acagtgtttg gattttttat
     5521 catgaaaacg gcgccaaagg cagcaccgga aattttgccc ggggcaaaca gcaaggcgcc
     5581 tgggtgtatt attatgaaac cggcgaaaaa tggaaagatg gctattttac tgatggtaaa
     5641 aaatcgggtt tgtggacaac atattatgaa gacggcaaaa aacaacgtga aggaacattt
     5701 gaaaatgata aggaaaacgg cttgtggacc agctggtatc gtaatggaca aaagcaggat
     5761 atgggcacat ttaatgatgg taaaatgcac ctgaaatggg aaggatggtt cgaaaaagga
     5821 aacaaaaaat atattgctga atacaacgac gggcaaaggt cgggaaaata cacactctgg
     5881 tgggaaaacg gcaatatgcg caccgagcag atgtacaaaa acgataaaaa aaatggtgct
     5941 tcgagcgaat ggtacgagaa cggaaaaatt acagaaagcg gctattatac caacgacctg
     6001 aaaagcggct actggagttt ttacgatcaa tatggtgtgc ttttccagga gcagggtttc
     6061 agccagggac gtcccgatgg aaaatttgtt gaataccatc agaatggcca gaaatcaagt
     6121 caggggctgt acgtgactgc cctgcgcgaa ggaacttggt actactggga taaggacggc
     6181 aaactgttat acaaggtttc gtttaccaaa ggtaagaagg ttagcgaaga aaacgccggc
     6241 aagccggaac cgaagaaata gcatgtaacg gtaattccgg ccgggaaaaa tcatgaattt
     6301 aacctgacgg aacgtcaacc
//
LOCUS       DAOSFP010000106         6313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_10852_length_6313_cov_4.357622, whole genome shotgun sequence.
ACCESSION   DAOSFP010000106 DAOSFP010000000
VERSION     DAOSFP010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10852_length_6313_cov_4.357622"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1386)
                     /locus_tag="PLI16_04345"
     CDS             complement(<1..1386)
                     /locus_tag="PLI16_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH83819.1"
                     /translation="MTSLRTLLYGALAFACLTSVTYGQKTVNLKDSIPVDPAVLIGKL
                     DNGLTYYIRQNKKPENRVELRLAVNAGSILENHSQRGLAHFTEHMCFNGTKTFSGNKM
                     IDYLQKYGITFGKEINAYTGFDQTVYMIQLPADNVSLLDTGMLIIEDWAHNVTFDSKE
                     IDKERGIITEEWRMGLGADDRMMKKWFPVVFKNSKYAERLPIGDIDVIKNAPYDTLRA
                     FYKSWYRPNLQAVVVVGDIDVKKMEEKVRQLFGGIQNPANTRPRPNIDIPDNTEPLIS
                     IVTDKEATNSMIFVIYKHKASVDYTLEDYKVALTADLYNEMLNARLKEITVQPDAPFI
                     MAQADYGNFLARSTDAYQLFAYPKENQIEKAFETLLLENERVRQFGFTATEFERTKEQ
                     MLNDFEKNSKEFDKTESENYADDYVENFLSQAIIPGAKSEFKLAKKFLPEISLDDVNA
                     LAAKWITNDNMA"
     gene            complement(1555..3249)
                     /locus_tag="PLI16_04350"
     CDS             complement(1555..3249)
                     /locus_tag="PLI16_04350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOH83820.1"
                     /translation="MENFIVSARKYRPATFDTVVGQHSITSTLKNAIKSQQLAQAFLF
                     CGPRGVGKTTCARILAKTINCLNLNENFEACNQCVSCKSFNELTSFNIHELDAASNNS
                     VDDIRTLVEQVRIPPQAGKYKVYIIDEVHMLSAAAFNAFLKTLEEPPAYAKFILATTE
                     KHKILPTILSRCQIFDFKRITVNDMANYLAYVANCENIEAEPDALHLIAQKADGAMRD
                     ALSIFDQIAISSDNKITFRAVLENLNILDYEYYFKMTVLILENKTFEALNLIDTVLEN
                     GFEGQVFLSGFAAHLRDLLVCKDDVTIKLLEASENIKNKYLMLARQCPEAFLTRNLEI
                     ANRFDFEYKNSNHKRLHLEMAFIKMCSNAQHVAVTDTSQNTEIKKKTEPVSTEVPKIP
                     EAPQKTQATQAIPSQPTVKKAVASAKSVISIKDETDDKKTEVNDVIINYGNEPYDLEK
                     IHQLWPEFISKFDKHPSFYTALKGDLPTQDHDGNIKFVFENNLTLNEFVSKKPEILEF
                     FRSALHNSSFDMYGEVAVKEDLQRKPYTPDEKYNEMNRKNPELKNFKEQLDLDIDY"
     gene            3437..5122
                     /locus_tag="PLI16_04355"
     CDS             3437..5122
                     /locus_tag="PLI16_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOH83821.1"
                     /translation="MLETKNNATTIKVRCLNNGVIRDIPRGTSLMQIAEILNVKLDYP
                     ILGALVNNCVEELSYEVYQPKKIGFIDITHPDGMRMYLRSLSFVLYKAVNDLFPNARL
                     RINHNISKGYFCSINHMEKELSDADILKIKARMHEIIEADIPFNNFVEETEVVIKKFE
                     ASGLTDKINILRHRGENYAKYYEIDGCIDSFYGYLVPSTGYVKVFDLNRYYDGMLLQL
                     PKKDDPGRVDTLIMQEKMFEILKEFSEWNQILDVTNVGELNKYISEGKSELLIKVSEA
                     LHEKKISQIADMINTRKDEVRVVLISGPSSSGKTTFGKRLAIQLLVNGIKPLNLSLDN
                     YFVSRDKTPLDENGQYDFEALEAIDIELFNNNLTELLQGNRIKLPKFSFESGSRFYNG
                     ETMQMQPENILIIEGIHGLNPQLTSTIESRAKFKIYVSALTTLNIDDHNRISTTDNRL
                     IRRIVRDFRYRKYSALDTISRWPSVVKGEEKHIFPFQEEADIMFNSAQIYELAVIKQY
                     AEHILMEVQSNNPEYSEAKRLLKFFSYFKSMDSRGIPPTSILREFVGGSSFLY"
     gene            complement(5339..>6313)
                     /locus_tag="PLI16_04360"
     CDS             complement(5339..>6313)
                     /locus_tag="PLI16_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOH83822.1"
                     /translation="LTQIDPVTGKALEGALSVFYEAGIANEVFPLLIFIAIGAMIDFS
                     PLFKNPLLLLFGAAAQFGIFLTMMGATLLGFDLKEAASIGIIGAADGPTSIFVSSRFA
                     PNLLGPISVAAYSYMALVPIIQPPVIRLLTTKKERCIHMEAHAKSVSKTSLIVFPIVV
                     TIFAGIIAPSSLALIGFLMFGNLIRECGVLNKLSLAAQNELSSIVTILLGISIASTMT
                     ADRFVRLDTLMIIGLGLVAFIFDTFGGVMFAKLMNIFLPSRKKINPMVGACGISAFPM
                     SARVIHGMGQKEDPTNYLLMHAVSANVGGQIGSVVAGGLILSLIPLFC"
BASE COUNT         1827 a         1320 c         1351 g         1815 t
ORIGIN      
        1 ggccatatta tcattggtaa tccatttagc agccagggcg ttgacatcgt cgagtgaaat
       61 ttcgggtaag aactttttgg ccagcttaaa ctcgcttttt gcgcccggga tgatggcctg
      121 gcttaaaaaa ttctccacat aatcatcggc ataattttct gattccgttt tatcaaattc
      181 tttggaattc ttttcaaagt cattcagcat ctgctctttg gtgcgttcaa actcggtagc
      241 tgtaaatcca aactggcgga ctctttcgtt ttcgagaagc agcgtttcaa aagctttttc
      301 gatttgattt tctttgggat aggcaaacaa ctggtaggca tcggttgaac gcgccagaaa
      361 attgccatag tcggcctgcg ccataataaa aggagcatcg ggttgtacgg tgatttcttt
      421 caggcgggcg ttgagcattt cattatacaa atcggctgtc agggctactt tataatcttc
      481 cagcgtataa tccacactgg ctttatgctt atagatgaca aatatcatcg agttggtggc
      541 ttctttgtcg gtaacaatgc tgatcagtgg ttctgtattg tcgggaatat caatgttggg
      601 ccgtgggcgg gtattggccg ggttttgaat gcctccgaac aactgcctga ctttttcttc
      661 catttttttc acatcgatat cacctaccac aaccaccgcc tgaaggttgg ggcggtacca
      721 tgatttataa aatgccctca gcgtatcgta aggcgcgttt ttaatcacat caatatcccc
      781 tatgggcagg cgttcggcat atttagagtt tttgaaaacc accgggaacc attttttcat
      841 catacgatcg tcggcgccaa gacccatgcg ccactcttcg gtaataatgc cccgttcctt
      901 atcaatctcc ttgctatcaa aggtaacatt gtgagcccaa tcttcgataa tcagcatccc
      961 ggtatcaagc aggctcacat tatctgcagg caactgaatc atatacactg tctgatcgaa
     1021 accggtatag gcattaattt ctttgccaaa ggtgattccg tatttctgca aataatcaat
     1081 cattttatta cctgaaaaag tcttggttcc gttaaaacac atatgttcgg taaaatgtgc
     1141 cagtccccgc tgactgtggt tttcgagaat tgatccggca ttgacggcca gccgcagttc
     1201 tacacggttt tcaggttttt tgttctgacg gatataatag gtcagcccgt tgtcgagttt
     1261 tccgattaac acagcagggt caacaggaat actgtctttc agattgactg ttttttgccc
     1321 gtaggtaacc gatgtgaggc acgcaaaagc aagtgcgcca taaagtaatg ttcgtaaact
     1381 tgtcatgtta aaaatttatt tttattattc gtttaatttt ttcgcagagg ttttaatgtg
     1441 tttcttttga tgtggctgga ctgtttttgt tacaattttt ctaacaatca cagacacact
     1501 attatccggc atcaccaagt gaaaatcata tcgcgggaag tttttttgaa aaaatcaata
     1561 gtcaatatcc aggtccagtt gttctttaaa gtttttcagt tcgggatttt tccggttcat
     1621 ctcattatat ttttcatccg gagtataggg ctttctttgt aggtcttctt tgacggccac
     1681 ctcgccgtac atatcgaaag aactgttatg cagggcgctt ctgaaaaatt caagtatttc
     1741 cggctttttc gaaacaaact cgttcaaagt caggttgttt tcaaaaacaa atttaatatt
     1801 gccatcgtga tcctgtgtgg gtaaatctcc tttgagagcc gtataaaaac tggggtgttt
     1861 atcaaatttg ctgataaatt cgggccacaa ttgatgaatt ttctccaggt cgtaaggctc
     1921 attaccataa ttaatgatga cgtcatttac ctcggttttt ttatcatcgg tttcgtcctt
     1981 tatggagatc acagatttgg ccgatgccac cgctttttta acagtcggtt ggctcggaat
     2041 ggcttgtgtg gcttgagttt tctgaggcgc ttccggtatt tttggaactt cagtgcttac
     2101 aggttcagtt ttttttttga tttccgtgtt ttgcgaggtg tccgttacag caacatgttg
     2161 cgcattactg cacattttaa tgaaagccat ttcgaggtgc aggcgtttat gattgctgtt
     2221 tttatattcg aaatcaaacc ggttggctat ttcaaggttt ctggtcagaa acgcctccgg
     2281 gcactgccgg gccagcatca aatatttgtt tttaatattt tcactggcct cgaggagttt
     2341 tatagtaaca tcgtccttac acaccagcag gtcgcgaaga tgcgctgcaa atcccgaaag
     2401 aaaaacctga ccttcgaagc cattttccaa cacagtatct atgagattga gcgcttcaaa
     2461 agttttattt tcgaggataa gcactgtcat tttaaaataa tattcataat ccagaatatt
     2521 aaggttttcg agtaccgccc ggaatgtaat tttattatcg gaagaaatag caatctggtc
     2581 gaaaatggaa agggcatcac gcatggcgcc atcggctttc tgagcaatca ggtgcagggc
     2641 gtcgggctcg gcctcaatgt tctcgcaatt agctacataa gccaggtaat tggccatatc
     2701 attgaccgtg attcttttaa aatcgaaaat ctggcaacgc gacaatatag taggaagaat
     2761 tttatgtttt tcggttgtgg ccagaataaa ttttgcatag gcaggaggct cttccagcgt
     2821 tttgagaaag gcattgaaag cggcagccga aagcatgtgc acctcatcga tgatatacac
     2881 tttgtattta ccggcttgcg gaggaatgcg cacctgttcc accagggtgc ggatatcgtc
     2941 aacagagttg ttggaggcag catcaagctc atgtatattg aatgaggtaa gctcgttgaa
     3001 tgatttgcag gaaacacatt ggttacaggc ctcaaaattc tcgttaaggt tcaggcagtt
     3061 gatagttttt gccagaatgc gggcacaggt ggtttttcct accccgcgcg gcccgcaaaa
     3121 taaaaacgcc tgcgccagct gctgcgattt aatggcattt tttagcgttg aggtgatgga
     3181 atgctgtcca acaaccgtat caaaggtagc aggacggtat tttcgggctg aaacaataaa
     3241 attctccatg cgaaagtttt aagccatagt tgatgagtac aaaaatatga aattttttcc
     3301 aatcaggatt cattcgctca aggtccggca taaaaaccgg atacttattt tgtttaaatt
     3361 tgcaaaatta ttgtaacacg aactacacaa aatacaaaac atattttata ttgccattta
     3421 attattattt atcaacatgt tagaaactaa aaataacgcc accactatta aagtcaggtg
     3481 cttaaacaat ggagtcatcc gcgacattcc ccggggtact tcacttatgc aaattgctga
     3541 aattttaaat gtcaaactag actatcccat acttggagcc ttggttaaca attgcgttga
     3601 agaactgtcc tacgaagtgt atcagcccaa aaaaatcggt tttattgata ttacccatcc
     3661 tgatggtatg cgcatgtacc tgagaagtct ttcgtttgtc ctgtacaaag cagttaatga
     3721 tttatttccc aatgcccgtt tacgcatcaa ccacaatatt tccaaaggat atttttgcag
     3781 tattaaccac atggaaaaag aactcagcga tgccgacatc ctgaaaataa aagcccgcat
     3841 gcatgaaatt attgaagcgg atatcccttt taacaacttt gtggaagaaa cggaagtggt
     3901 gataaaaaaa tttgaagcca gcggactgac agacaagata aatatcctga ggcaccgggg
     3961 tgagaactac gcaaaatatt atgaaattga tggctgtata gattcctttt atgggtatct
     4021 ggtgccatcc acgggatacg ttaaagtttt tgacctgaac aggtattacg acgggatgtt
     4081 gctgcaactt cctaaaaaag acgaccccgg aagagtggac accctgatca tgcaggaaaa
     4141 aatgtttgag attttaaaag aattctctga atggaatcag attctcgacg ttaccaacgt
     4201 gggtgaactc aataagtata tttcagaagg caaatcggaa ctattgataa aagtgtcgga
     4261 agcattgcat gagaaaaaaa tcagccagat agccgacatg attaatacac gtaaagacga
     4321 agtcagggtg gttcttatca gcggcccatc gtcgagcgga aaaaccacgt tcggaaaaag
     4381 gctggccata caactgcttg tcaacggtat taaacctttg aatttatcgc tcgacaatta
     4441 ttttgtgagc cgcgacaaaa ccccgctgga tgaaaacggc cagtacgatt tcgaagccct
     4501 ggaagccatt gatatagaat tatttaacaa taaccttaca gagcttttgc aaggcaacag
     4561 aataaaatta ccaaaatttt cttttgaatc gggttcgcgt ttttataacg gtgaaaccat
     4621 gcagatgcaa cccgaaaaca tcctgataat tgaaggtatc cacggtttga accctcaact
     4681 aacctccacc attgaatccc gggctaaatt caagatttat gtttcagccc tgaccacctt
     4741 aaacattgat gaccacaacc ggataagcac cacggacaac cggctgatac gacgaatagt
     4801 gcgtgatttc aggtaccgga aatattcggc cctggacacc atttcgcgct ggcccagcgt
     4861 ggtcaaagga gaagaaaaac atatttttcc ttttcaggaa gaggccgaca tcatgtttaa
     4921 ttcggcccag atctatgaat tggctgttat caaacagtat gctgagcaca ttctgatgga
     4981 ggtacagtca aacaaccctg aatattcgga agccaaaagg ctgctgaagt ttttcagtta
     5041 cttcaaatcc atggattctc gcggcattcc gccaacatcc atattaaggg agtttgtggg
     5101 aggcagtagt tttctgtatt aacaaacgca tcgctacgcc acctgtcagg gcataattct
     5161 attaatgaac caggacaaaa aggtattaat cagccttttg tttatgggga aataacttat
     5221 ctattgcctg aatggcaaag tagaaaatta tcataaaaat aaatatccct aacattccaa
     5281 ggccggtaac ttccaatgct gcttcaaaat tattcatcat agtttttatc ctccgtattt
     5341 agcaaaataa aggaattaat gaaaggatga gaccgccggc caccacggat ccgatttgtc
     5401 cgcccacgtt ggcgctaacg gcatgcatca gcaaataatt ggtggggtct tctttttgtc
     5461 ccataccatg aattacacgg gccgacatgg ggaaggcaga aataccgcag gcacccacca
     5521 tcgggttgat tttttttcgt gaaggcagga agatgttcat aagcttggca aacatcacac
     5581 ctccaaaagt atcgaagata aaagcaacca gacccaggcc tatgatcatc agggtatcca
     5641 gccttacaaa gcggtcagca gtcatggtgc tggcaatact gattccaagc aatatggtta
     5701 cgatactgga taattcattc tgagctgcca acgagagctt attcagcacg ccgcattctc
     5761 tgataaggtt tccaaacatc agaaatccga taagtgccag cgatgaaggc gcaataatac
     5821 cggcaaagat ggttacaaca ataggaaaaa ctataagaga ggttttcgaa acacttttgg
     5881 catgtgcttc catatgaatg caacgttctt tcttggtagt cagcagacgg attaccggag
     5941 gctgaatgat aggtactaag gccatgtagg aataagcggc cacagaaata ggacctaaca
     6001 aattgggtgc aaacctcgac gagacgaaaa tggatgtggg gccatcggct gcaccgataa
     6061 tacctataga agcggcttcc ttcaggtcga atccgagcaa tgtggcaccc atcattgtca
     6121 ggaatatccc aaactgggcg gccgcaccga ataataataa caaaggattt ttgaataaag
     6181 gcgaaaaatc tatcatcgcc ccgatagcaa tgaaaataag caacgggaac acttcattgg
     6241 caataccggc ttcgtaaaaa acagacaaag ctccttccaa cgcttttccg gttaccggat
     6301 caatttgtgt aag
//
LOCUS       DAOSFP010000107         6252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11005_length_6252_cov_3.472648, whole genome shotgun sequence.
ACCESSION   DAOSFP010000107 DAOSFP010000000
VERSION     DAOSFP010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11005_length_6252_cov_3.472648"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="PLI16_04365"
     CDS             complement(<1..975)
                     /locus_tag="PLI16_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOH83823.1"
                     /translation="MLNSKIFSIVFILLFGLIYAKTNDLPDNNPDNSPSVNKWVDSVF
                     DKLTPEQRIAQLFMVAAYSNQSLKANELVTELIVKYNIGGICFFQGGPVTQARYTNYW
                     QSLAQTPLLIAIDGEWGLGMRLKDSTMSFPKQMTLGAIRDNKLIYAMGEEIGKQCKRM
                     GVSMNFAPVVDINSNPQNPVINYRSFGEDKKNVTNKALEYMKGMQNRGILATAKHFPG
                     HGDTDKDSHTELPTIHHSKELIDSVDLYPFRKLAQNNLAAVMAGHLFVPALDTTSDQP
                     SSLSPKIIHDVLSVEMKFKGLIITDALGMKGASEKAKPGEIELKALLAG"
     gene            complement(1193..2779)
                     /locus_tag="PLI16_04370"
     CDS             complement(1193..2779)
                     /locus_tag="PLI16_04370"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH83824.1"
                     /translation="MKKKLLFTFLFCGFALLSQAQWTIQASGFPVQNRGIKHLCAVDT
                     NVIWATAYDGSVANAPQIQEFTRTTNGGVKWKYGAIPGYAGHGLSMISAVDSMNAWIP
                     LWYSTGGGTILHTADGGATWTPQTTATFDSPNGFPNVVHFWNVNEGFCMGDPNGGYFE
                     IYTTVDGGTTWSRVPEADIPPNSAQEYGTTGYYDVVGNVVWFTTNKGRIYKSIDKGYH
                     WTVGTIPNVTDQMQISFRDINYGIVRANMSPFNTYFTNDGGNTWQQLFWNGNFYNNDF
                     CYVPGTTNTFISAGADYTNNFMGTSISTDGGFNWNTIPLSDTTQFLTVDFVDINHGWA
                     GAFNTDSVTGGMWKYTGNLFVEDPCAGLVAMYVKSADTLDLNISGVCDFTDLSSGNPS
                     GWSWDFGDGGSANVQNPSHTYTAAGTYNIELTVTKDTCTEVYSETIVVVNTAGISTYS
                     EGPAVEIWPNPAKDYIYVASPAEVHRVEIYNSIGQSAIHSINNEKFIKINISDYLPGV
                     YFVRIFTSQGRTGGRFITTK"
     gene            complement(2920..3558)
                     /locus_tag="PLI16_04375"
     CDS             complement(2920..3558)
                     /locus_tag="PLI16_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83825.1"
                     /translation="MKKLFLVLIGIFAMVQYGYSQANEGSAEFDKVERPAVIGEFAYT
                     ADVVEESIIADLKEKGIKKYSTKKGFMIFEEAVFTQISANKINFYVKVEEKKKEKDKS
                     IVTFLVSYGLDNFISSAMDANIINNTKSYMNGLLPKFAATKLGRDIDAQQKVYDKAVK
                     DYENLVGDGESLAKKKADIENDITKNKSDQEAQKAVVAKEKANLESLQGQRK"
     gene            complement(3617..5449)
                     /gene="mutL"
                     /locus_tag="PLI16_04380"
     CDS             complement(3617..5449)
                     /gene="mutL"
                     /locus_tag="PLI16_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOH83826.1"
                     /translation="MPDIIKILPDNVANQIAAGEVIQRPASAVKELLENAIDAGADDI
                     QLIIKDAGKTLIQVVDNGCGMSETDARLCFERHATSKIYEAGDLFKIHTYGFRGEALA
                     SIAAIAQLEMKTKKKSDEVATSILIEGSVVKEHTSCSAADGTSIAVKNLFFNVPARRN
                     FLKSDATETSYIIEEFNRVALVYPEKSFALYNNGKLVSKLEKMPLRQRINAVFGHNYG
                     ERLISIDESTTVAEIKGFIGKPEFARKTRGEQYFFVNRRFIKHPYLNHAVDKAYAELI
                     PEQSYPSYFIYITIDPKNIDVNIHPAKTEVKFYDEKTLYSILKAGIRKAFGSFNITPS
                     IDFDVDRALDFGATNFNREEGIKFPTVKINPDYNPFRNNPHSSTSDEVSKQHDKNLKN
                     WETLYELGKEISASKNADEHTNPEIAIPMAHENASFFCFGKKYLLTPMKSGLMFIDIV
                     QARERILYEKFLETAENQNPVCQRLLFPQTISLNPADAELLADILKEMRLLGFDIETF
                     GKNTFVVNGLPADFSDQDINDTIERLLESYKKNQLEASYEKKVNLIRSLAKNMAPRTT
                     LQLTDEAMQALAGQLFACSMPDTSPSGKKILFILSNEEIEKKFK"
     gene            5652..>6252
                     /gene="aqpZ"
                     /locus_tag="PLI16_04385"
     CDS             5652..>6252
                     /gene="aqpZ"
                     /locus_tag="PLI16_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HOH83827.1"
                     /translation="MKKLIAEFIGTLWLVLGGCGSAMLAAGYPELGIGFAGVALAFGL
                     TVLTMVYAIGHVSGCHLNPAVSLGLWIAGRFDKKELLPYIAAQVLGALAGAGILYLIV
                     SGKEGFELGSFAANGYGELSPGGYDMIAALVTEIAMTFMFLIVILGATHAKAPKGFAG
                     VAIGLALTLIHLISIPVTNTSVNPARSTSQAIFAGTEYLS"
BASE COUNT         1635 a         1388 c         1342 g         1887 t
ORIGIN      
        1 gccggccaat agggctttca attctatttc accgggtttt gctttttcgc tggcgccttt
       61 catgcctaag gcatcagtaa tgataaggcc cttgaatttc atttctaccg acaacacgtc
      121 atgaataatt ttcggtgaca gggaggatgg ctggtccgaa gtggtgtcaa gggccggaac
      181 aaacaaatgt ccggccatca cagccgccag gttattttgt gctaattttc ggaatgggta
      241 caggtcaaca ctgtctatta gttctttgct gtgatggatg gtgggcaact ccgtatgcga
      301 gtctttatcg gtatcgccgt ggcccggaaa atgtttggct gtggcgagta tgcctctatt
      361 ctgcatgcct ttcatgtatt ccaaagcctt gttggttaca ttttttttat cttcaccaaa
      421 actgcgatag ttgataacag ggttctgggg attggaattg atatctacca cgggagcgaa
      481 attcatgctt acccccattc ttttgcattg tttacctatc tcttcgccca tagcatagat
      541 gagcttattg tcgcggatgg cgcccagtgt catttgcttg ggaaaagaca tggtgctgtc
      601 tttcaaccgc atacccaatc cccattcgcc gtctatggca attaacagag gggtctgggc
      661 gagcgattgc cagtagttgg tgtaacgcgc ctgtgttacc ggaccgccct gaaaaaaaca
      721 aataccgccg atattatact ttacaataag ttcggtaacc agttcattgg ccttaaggct
      781 ctggtttgaa taggccgcca ccataaacaa ctgagctatt cgctgttcgg gagtaagttt
      841 atcaaaaaca gaatcgaccc atttattaac agaaggggaa ttatcaggat tgttgtcagg
      901 aagatcattg gttttcgcat aaatcaatcc aaacaacaaa ataaagacta ttgaaaaaat
      961 tttggaattc aacattgcta attattgaag tggtaaaaat atcatttcat aatgatagtt
     1021 ttaagtacag aacaaatatt tattaacttt tgaatgttgt taacctgcct gctgcccgca
     1081 acgtccgcac aaattcatca ttttcacttc gtgcatttag gaatagtcat gctaaaatgt
     1141 aaaacaaaaa aagctgcagt ttcctacagc ttttctctct ggttaatgtg ctttattttg
     1201 tagtaataaa gcgccctccg gtgcgcccct gcgaggtaaa gatccttaca aaataaaccc
     1261 ccggcaggta atcgctgata tttattttaa tgaatttttc attattaatg gagtgaatag
     1321 ccgactgtcc tatgctgtta tatatttcca cacgatgaac ctctgccggc gatgcaacgt
     1381 aaatataatc ctttgccgga ttaggccata tctccacagc cggtccttcc gagtaggttg
     1441 atattcccgc agtgttgaca accactattg tctcggaata tacttcagtg caagtatcct
     1501 tcgttactgt aagttcgata ttataagtgc cggcggctgt ataagtgtgg ctaggatttt
     1561 gcacattggc cgaacctccg tcaccgaaat cccatgacca tcctgaagga ttgcctgagc
     1621 tcaggtcagt aaaatcgcat actccggaaa tattcaggtc gagggtgtcg gcacttttga
     1681 catacatggc caccaggccg gcgcagggat cctcaacaaa aaggttgccg gtatatttcc
     1741 acatgcctcc cgtaacgcta tcggtgttga aagcgccggc ccatccatga ttgatatcaa
     1801 caaaatcgac ggtaagaaac tgcgtggtat ctgacagggg aatggtattc cagttaaaac
     1861 cgccgtcggt gctgatggaa gtccccataa aattattggt gtaatcagcc ccagcagaaa
     1921 taaaggtatt ggtagtcccc ggcacataac aaaaatcgtt gttgtaaaaa ttcccgttcc
     1981 agaacagttg ctgccaggtg ttgccgccgt cgttggtaaa atatgtatta aaaggcgaca
     2041 tattggctct tactatgcca taattgatgt ccctgaatga aatctgcatc tggtcggtaa
     2101 cgttgggtat agtgcccaca gtccagtggt agcccttgtc gatggattta tagatacggc
     2161 ctttgttggt ggtaaaccac actacgttac ccaccacatc ataatacccc gtagtgccat
     2221 attcctgtgc agaattggga ggaatatcgg cttcaggaac cctgctccag gtagttcctc
     2281 catcaacagt ggtatatatc tcaaaatacc cgccattggg gtcgcccata caaaaacctt
     2341 catttacatt ccagaaatga accacattgg gaaacccgtt gggtgaatca aaggtggcgg
     2401 tagtctgtgg agtccatgtg gcacctccgt cagcggtatg cagtattgta ccgcctccgg
     2461 tggaatacca caagggtatc caggcattca tcgagtcaac ggcgcttatc atcgacaagc
     2521 catggcctgc atatccgggt atggcaccgt atttccattt tacacctccg ttggtggtac
     2581 gcgtaaactc ctgaatctgg ggagcattgg caacactgcc gtcgtatgcc gtggcccata
     2641 tcacattggt gtcaacggca cacaggtgtt ttatgccgcg gttttggaca ggaaaaccac
     2701 tggcttgtat ggtccactga gcctgggaga gcaaagcgaa accacagaat aaaaaggtaa
     2761 aaagtaattt tttcttcata atgtttttta ttggttgatg tgataaaaat aaaacaaaaa
     2821 acgtttgcag tcaaataaaa aatatttctt cggtgtttta acaaaaaaaa ccgccggaat
     2881 accatcccgg cggtttttct gtttattata accaaaaggt tactttctct gtccctgtaa
     2941 tgattccagg ttggcttttt cttttgccac aacagctttc tgcgcctcct ggtctgattt
     3001 gtttttggta atgtcattct caatatctgc ctttttcttg gcaagcgatt cgccatctcc
     3061 aacaaggttt tcgtaatcct tcacggcctt atcataaact ttctgctgcg catcgatgtc
     3121 cctgccaagt ttggtagcgg caaatttggg taataagcca ttcatgtagc ttttagtgtt
     3181 gttgataata ttggcatcca tggcgctgct gataaaatta tccaggccat aactcacgag
     3241 gaaagtaact attgacttat ctttttcttt tttcttctct tcgactttta cataaaagtt
     3301 gattttgttg gctgagattt gagtaaaaac tgcttcttca aaaatcataa aacctttttt
     3361 tgtactgtat ttctttatgc ctttttcttt caggtcggcg ataattgatt cttctaccac
     3421 atcggcggta taagcaaatt ctccaatcac ggcagggcgt tccactttat caaattctgc
     3481 gctgccttca tttgcctggg agtacccgta ctgcaccatg gcaaaaattc cgattaacac
     3541 taagaataac tttttcataa ttcaagattt tttaatttaa cacaaaaata catgattttt
     3601 ataaatatta tcaggtttat ttgaatttct tttcaatttc ttcattactt aaaatgaaaa
     3661 gtatcttttt tcccgaaggc gatgtatccg gcatagagca ggcaaacaac tgtcctgcca
     3721 acgcctgcat ggcttcgtca gtaagttgca gtgttgtcct gggagccata ttttttgcaa
     3781 gagagcgtat caggtttact ttcttttcat aggatgcctc cagttgattc tttttataac
     3841 tttccagcag tcgttcgatg gtatcattaa tatcctgatc agaaaaatca gctggcagtc
     3901 cattcaccac aaaagtgttt ttgccaaaag tttcgatatc aaaacccaac aggcgcattt
     3961 ctttcagaat atccgccagc agctcggcat ccgcaggatt caacgagata gtctgcggaa
     4021 atagcaggcg ctgacacacc ggattttggt tttcggcagt ttcaagaaat ttctcgtaca
     4081 atatacgttc acgggcttgc acaatatcaa taaacatcaa gccggatttc atgggtgtaa
     4141 gcaaatattt tttgccaaaa caaaaaaatg atgcattttc atgcgccatc ggaatcgcta
     4201 tctcaggatt agtatgttca tctgcatttt tacttgccga gatttctttt cccagctcat
     4261 aaagcgtttc ccagtttttc agatttttgt cgtgctgttt cgacacctca tcagaagtgc
     4321 tgctgtgagg gttgtttctg aaaggattgt aatccgggtt gattttcacc gtcggaaatt
     4381 ttattccttc ctccctgttg aaattggtgg cgccaaaatc cagggcgcgg tccacatcaa
     4441 aatcaatgct gggagtaatg ttaaacgacc cgaacgcctt tctgatgccg gcctttaaga
     4501 tgctatacag tgttttttcg tcgtaaaact tcacttcagt ttttgcgggg tgaatattta
     4561 catcaatatt tttgggatcg atggtaatgt aaataaaata cgaaggataa ctctgctcgg
     4621 gaatcagttc ggcataggcc ttatccacgg catgattcag gtagggatgt ttgataaagc
     4681 gcctgttgac aaaaaaatac tgctctcccc ttgtcttgcg tgcaaattcg ggtttaccta
     4741 taaaaccctt gatttcagcc actgttgtgg attcatcgat ggagataagc cgttcgccgt
     4801 aattatgacc gaacaccgca ttgatacgtt gcctcagcgg cattttttca agttttgaaa
     4861 ccagctttcc gttgttatac aaggcaaaac ttttttcggg atacaccagt gccacccggt
     4921 taaattcctc tatgatgtaa ctggtttcgg tggcatccga cttcagaaag ttgcgccgtg
     4981 ccgggacatt aaaaaagagg tttttcaccg caatggaagt gccatcggca gcactgcagg
     5041 aggtatgttc cttaacaaca gaaccttcaa ttaaaatgct ggtagccacc tcgtcggatt
     5101 tctttttggt tttcatttcc agttgggcta tcgcagcaat agaggccagc gcttcaccgc
     5161 gaaacccgta tgtgtggatt ttaaacaagt cgcccgcttc atagattttt gaggtagcat
     5221 ggcgttcaaa acacaaacgg gcatcggttt ccgacatgcc gcaaccgttg tccaccacct
     5281 gtatcaaggt tttgccggcg tctttaatga taagctgtat atcatcagca ccggcatcta
     5341 tggcattctc cagcaattct tttaccgccg aagccggtcg ttgtattact tcgccggcgg
     5401 caatctggtt ggctacatta tcaggcaata ttttaataat gtcgggcata taaacaatta
     5461 tattgcaaaa ttacaatttt tttccgtatc acgacacggc agacattatc tgcaaagaca
     5521 ctgatgataa agtaccatag tgcctttctg cctgaaaaac agttacgatg tgatatgttt
     5581 gctaaaaaaa ttttttttct taatgaaaaa aatatacttt tgttatataa agtaaccaaa
     5641 aaagtaaaat catgaaaaaa cttattgctg aatttatcgg aaccttatgg ctggttcttg
     5701 gcggctgcgg aagcgccatg ttggcagctg gttatcccga actgggtatc ggctttgccg
     5761 gagtggctct tgcttttggg cttactgtac ttaccatggt atatgccatc ggccatgttt
     5821 cgggttgcca cctcaaccct gctgtatcgt tagggttatg gattgcggga cgattcgaca
     5881 agaaagaact gctgccatac atagctgccc aggtgttggg tgctttggcc ggcgccggta
     5941 ttttgtatct tatcgtcagc ggaaaagaag gttttgagtt aggatccttt gcggccaacg
     6001 gttatggcga actttcgccc ggcggctatg atatgatagc ggctttggtg acggaaattg
     6061 ctatgacctt tatgtttctg attgtcatcc tgggtgccac tcatgccaaa gcgcccaaag
     6121 gttttgccgg tgtggccata ggccttgcat taacccttat ccacctgata agcatcccgg
     6181 ttaccaacac ctcggtaaac cctgcccgaa gcaccagtca ggccatattt gcgggcacgg
     6241 aatacctgag cc
//
LOCUS       DAOSFP010000108         6216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11093_length_6216_cov_3.571660, whole genome shotgun sequence.
ACCESSION   DAOSFP010000108 DAOSFP010000000
VERSION     DAOSFP010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11093_length_6216_cov_3.571660"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /locus_tag="PLI16_04390"
     CDS             complement(<1..1847)
                     /locus_tag="PLI16_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HOH83828.1"
                     /translation="MKKDTAQTKLFDEFPPIDTQLWEEKIQADLKGADYEKKLIWKTI
                     EGLKIKPYYRAEHTENLSHLQALPGEYPFVRGYRANSNVWRIREDIVVTDVAKANADA
                     LKAISRGATAITFKVKELAYREEMATLLKGINMEEIAVHFFSSFSYSLLANLLIEYVK
                     NSGFKPDKIKGSFNFDSIGYYLLHGEYYNSKEDNFNEAAALIKQMKANLPQFRIINVN
                     GVHFMNAGATAVQELAYVLASANEYAAQMSDRGLDIDDVFADIQLTFGIGSNYFMEIA
                     KLRAARLLWARMAEQYKPARPASLTVAIHACSGTWNKSVFDPYVNLLRLTTECMSAAI
                     GGANAITVLPFNNTYAEADEFSARIARNIQIILKEEAYLDKVIDPAAGSYYLESLTDE
                     LAQLSWEYFKRIEEKGGMLTIMESGSLKQEIEYTAAQRNMDIAMRKTPVLGTNIFPNL
                     KETMLDQIQISRGADEHPYLKCYRGAEMFEYIRLSTEQFVKKGNKRPKVFLATYGNLA
                     MRKARAGFTTNFFGCAGYEISDEFVMKDIAATVQDIKNYDGNIVVICSSDEEYAQQAG
                     ELVSAVKQFNPQIKVIVAGYPKELTEQLKSAGVDDFIHVRVNVAETLAG"
     gene            2149..3129
                     /locus_tag="PLI16_04395"
     CDS             2149..3129
                     /locus_tag="PLI16_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOH83829.1"
                     /translation="MNDLYPLKFTPVFKEKIWGGHKIDQVLKQHHPHLNNCGELWAIS
                     GLTDSPSMVSNGFLRGNSLNDLVEVYMGDLVGDGIYDKFGDEFPLLFKFINAEDFLSV
                     QVHPGDQLAMRRHSCSGKAEMWYVVAADPGAELISGFNRKTSKEEYLQHLAGKSLKDI
                     LNFEKVGVGDVFNVPAGHVHAIGPGILLAEIQQASDITYRIFDWDRIDQAGMTRELHT
                     DLALDAINFDEKESYKSHPPVTKNRPVLLQETEFFTVNLLDLDNALEQDFYEKDSFVA
                     YMCLEGAFTVDCGESRENVSIGESLLVPAMIKKFTIIPQPSCRILETYVK"
     gene            3137..3991
                     /locus_tag="PLI16_04400"
     CDS             3137..3991
                     /locus_tag="PLI16_04400"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOH83830.1"
                     /translation="MKIRKPVLKRKSQPVMEGAGVRLRRALGYHDVPAFDPFLMLDDF
                     RNDHPEDYIKGFPWHPHRGIETITYMLKGEVEHQDSMGNKGVISAGDVQWMTAGSGII
                     HQEMPQPGGDGVMLGFQLWANLPASRKMMKPRYQEVKSSEIPEIVTSGNCSVRVICGN
                     FDSTAGPVKDIITDPVFLDVSVSAAGEFMLPTPADDNVFCYVIEGDGYFGEEGSGVEK
                     AENLDLLLFSEGGQFRVHTLGNAVRFLFLMGRPIKEPVAWGGPIVMNTQEELQTAFEE
                     FDNGTFIK"
     gene            complement(3997..5973)
                     /locus_tag="PLI16_04405"
     CDS             complement(3997..5973)
                     /locus_tag="PLI16_04405"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH83831.1"
                     /translation="MPRNNKNILLLLILLNLGMFSFAQDENPCTELANKKAKKVYNEA
                     FENFRNRRFIDASAQFKEVLEYEPQCGQCYFFLGMINFKKADYNLTAAQNYFEKSVEL
                     CPDFDVYVYYYLGQIYYGAEKWEQAENALKKFLKDPEKIENDAELNDASAMYKWAVFY
                     NKAYSNPVPFNPRYIKGVSSVSDEYIPSISPDGEYMFYVRVIELPPNKNDLVPKPVYK
                     EKFYYSEKKNGQYDNGKEMPYPFNVNDYEGGATLTIDNNELYYSVRKYKGSYFNGDIY
                     YSRKDESGTWSEIAPVENINNPDTWESMPSISSDGKTLYFVSDRPGGIGGLDLYRSVK
                     GEDGIWGKPENLGPQINTKNNEKTPFLHTDSQTLYFSSDSPDLMGLGGFDIYYSRIQA
                     DGSWKKPANLGYPINSAADDAGFFVSLDGKTAFFSSTKLVGVGGWDVYEFDLYADARP
                     EEMKLVKGDVKDETSEQPLTARIEIKNVETKKITKIPVDSLTGKYVIAINTRENFVLT
                     VKKDDYAYESKLIPANINERSEPVQELNFEIKPIEVGKSYQLNDIYFASNSYELNHES
                     EAILDGFIEFLTENPKIKTAIHGHTDNIGKDEDNMLLSDNRSKSVYNYLTEHGIAASR
                     LSYKGFGETKPVADNDTEAGRAKNRRTEFVILEK"
     gene            complement(5957..>6216)
                     /locus_tag="PLI16_04410"
     CDS             complement(5957..>6216)
                     /locus_tag="PLI16_04410"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004337314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOH83832.1"
                     /translation="TNNSGGIQGGISNGEDIYFSVAFKPVPGIKIKQKTVNRQAQTVE
                     FSTGGRHDVCFVPRAIPVVEAMTALVVTDSLFKQQVYAEKQ"
BASE COUNT         1518 a         1362 c         1510 g         1826 t
ORIGIN      
        1 tatccggcca gtgtttctgc aacattgaca cgtacatgga taaaatcgtc cacccctgcg
       61 gattttaatt gttctgtgag ctctttggga tagccggcaa caataacctt aatctgtggg
      121 ttgaattgtt tcaccgcgct gaccagttcg cccgcctgct gggcatattc ctcatcggag
      181 ctgcagataa ccacgatatt gccatcatag ttttttatgt cctgcaccgt ggcggcaata
      241 tctttcatca caaattcatc gctgatttcg taacctgcac agccgaaaaa gttggtggta
      301 aaaccggctc ttgcttttcg catggccagg ttgccgtagg tggccagaaa aactttcggc
      361 cgcttatttc ctttttttac aaattgttcg gtggaaagcc ggatgtattc aaacatttcg
      421 gcaccacggt agcatttcag gtaagggtgt tcgtctgcac cacggctgat ttgtatttga
      481 tcaagcattg tttccttcag gttggggaag atgttggttc ccaacacagg agttttgcgc
      541 atggcgatat ccatgtttcg ctgggcggcg gtatattcga tttcctgttt caggcttccc
      601 gactccatga tggtgagcat accgcctttt tcttcgatgc gtttaaagta ctcccatgat
      661 aattgcgcca gctcatcggt aagcgattct agataataag agcctgcagc agggtcgatg
      721 accttgtcga ggtaagcttc ttcttttaaa atgatctgga tgttacgggc aatacgtgcg
      781 ctgaattcgt cggcttccgc ataggtatta ttaaaaggca gcacggtaat ggcgtttgct
      841 ccgcctatgg ctgccgacat acattcggta gtcagacgca gcaggtttac ataaggatcg
      901 aacaccgatt tgttccaggt gccgctgcat gcatgaatgg ccacagtgag ggaggcaggc
      961 cgggcgggtt tgtattgttc ggccatgcgg gcccacaaca accgtgcagc gcggagtttg
     1021 gctatttcca tgaaataatt ggaacctatg ccgaaagtga gttgtatgtc ggcaaaaaca
     1081 tcgtcaatgt caaggcctcg gtcgctcatc tgggcggcgt attcgttggc tgaggcaagc
     1141 acataagcca gttcctgaac agcggtagcc cctgcattca taaaatgaac accgttgacg
     1201 ttgatgatgc ggaactgcgg tagattggcc ttcatctgtt ttatcagggc ggcagcttcg
     1261 ttgaagttgt cttctttgct gttatagtat tcgccatgca gcaagtaata tcctatggaa
     1321 tcaaagttga aagagccttt gattttatcg ggcttaaaac ctgagttttt cacatattca
     1381 atcagcagat tggccagcag ggaataggaa aacgatgaaa agaaatgtac ggcaatttct
     1441 tccatgttga tgcccttcag cagggtagcc atttcttctc tgtaggcaag ctctttcact
     1501 ttgaaagtaa ttgcggtggc gcctctcgaa atcgctttta atgcgtcggc gttggctttg
     1561 gcaacatctg ttactactat atcttcgcgg atgcgccata cgttggagtt tgccctgtag
     1621 ccgcgaacaa aaggatattc gccgggtagc gcctgcagat gcgaaaggtt ttcggtgtgt
     1681 tcggcccggt aataaggttt tattttaagg ccttctatgg tcttccagat aagttttttt
     1741 tcataatctg cgcctttcag gtcggcctga attttttctt cccacaattg ggtgtctatg
     1801 ggaggaaact catcaaaaag tttggtttga gcagtgtctt ttttcattgc gtaatttttt
     1861 aacggtattt cgtcagaaat tcaaggcaaa aatacaaata tattagtgca aaaaacaggt
     1921 ttattgatat taaaatatta tttatttatt cttgatcttg tgagattctg gatttatagc
     1981 gttttcaggc tttcggaaga gacttgtcag tttcagttaa caattttcta tgatgatatt
     2041 accatatcgc actgatgtat gacaaaccaa ctaaaaacat ccaaaaagta caagacagta
     2101 ttttctatac ccgccctttt tactatcttt gctttataaa cttattttat gaatgattta
     2161 tatcccttga aattcactcc tgtttttaaa gaaaaaattt ggggcggcca caaaatcgac
     2221 caggtgctga agcagcatca tcctcatttg aataattgtg gcgaattgtg ggccatctcc
     2281 ggactgactg attcgcccag catggtttcc aatggatttc tccgtggcaa ttccttgaat
     2341 gatttggtgg aggtatatat gggtgatttg gtgggcgatg gcatatatga taaatttggc
     2401 gatgagtttc ccttgttgtt taagtttatc aatgccgaag attttctttc ggtgcaggta
     2461 catcccggtg accagcttgc catgcgccgc cactcatgca gcggcaaggc cgaaatgtgg
     2521 tatgtggtgg cagccgaccc cggcgccgaa ctgatcagcg gatttaacag gaaaaccagc
     2581 aaggaggaat acctgcaaca cctggccggc aaatcactta aagatatcct gaactttgaa
     2641 aaagtcgggg tgggcgatgt gtttaacgta ccagctggac acgtacatgc tatcggaccg
     2701 ggtattttgc tggccgaaat ccagcaggca tccgacatca cttaccgcat cttcgactgg
     2761 gaccgtatcg atcaggcggg aatgacccgc gagctgcata ctgacttggc tcttgatgca
     2821 atcaattttg atgaaaaaga gtcttataaa tcccatccgc ctgttacaaa aaaccggccg
     2881 gtactgctgc aggagactga atttttcacg gtcaacctgc tcgacctgga taatgctctt
     2941 gaacaagatt tttatgaaaa agattcgttt gttgcttata tgtgcctgga gggagctttt
     3001 acagtggact gtggcgaaag ccgcgaaaac gttagtattg gagaatcgct gctggtgccg
     3061 gccatgatta aaaagtttac cattatccct cagccaagtt gcagaatcct tgaaacatac
     3121 gtcaaataaa aacattatga agataagaaa acccgtatta aaacgaaaaa gtcaacccgt
     3181 tatggaaggc gcaggcgtta ggttgcgcag ggcattagga taccacgatg taccggcttt
     3241 cgatcctttt ctgatgctgg atgatttccg caacgaccat cctgaggatt acataaaagg
     3301 atttccctgg catccgcacc ggggaataga aacaatcacc tatatgctga aaggcgaagt
     3361 ggaacaccag gacagcatgg gcaacaaagg ggtgatttcg gccggcgatg tgcagtggat
     3421 gacggccggc agcggtatca tccatcagga aatgcctcag ccaggaggcg atggcgttat
     3481 gctcggcttt cagctttggg ctaatcttcc ggcctcacgc aaaatgatga aaccccgcta
     3541 ccaggaagtc aaaagcagcg aaatacctga aattgttacc tccggcaatt gcagtgtacg
     3601 ggttatctgc ggaaattttg acagtactgc agggccggta aaagacatta ttactgatcc
     3661 ggtattcctg gatgtgagtg tgtcggcagc aggcgaattt atgttgccaa ctcctgctga
     3721 tgacaacgtt ttttgttatg ttatcgaagg tgacggatat ttcggcgaag aaggttccgg
     3781 tgttgaaaaa gctgaaaacc tggatctgct cctgttttcg gaaggtggcc agtttagggt
     3841 gcataccctc ggaaacgcag tgcgttttct gtttctgatg ggtaggccca taaaagaacc
     3901 ggtggcctgg ggagggccca tagtgatgaa cacgcaggaa gaactgcaga ccgctttcga
     3961 agaatttgac aacgggacgt ttatcaaata aactttttat ttttcaagta tcacaaactc
     4021 tgtgcggcgg ttttttgccc tgccggcttc ggtatcgttg tctgccacgg gcttggtttc
     4081 tccaaaaccc ttgtaggaaa gtcgtgaggc cgctatgcca tgttcagtaa ggtagttata
     4141 aaccgatttg gaacggttgt ccgaaagtaa catattgtcc tcgtctttgc ctatattgtc
     4201 ggtgtggccg tggatggcgg tttttatctt cgggttttcc gtcaggaact cgatgaaacc
     4261 gtcaagtatg gcttctgatt cgtggtttag ctcataagag ttggaagcaa aatatatgtc
     4321 gttgagctga tatgatttgc caacttcaat gggttttatc tcaaaattca gctcctgcac
     4381 gggttccgac cgttcgttga tgttggcggg gatgagtttc gactcgtagg cgtagtcgtc
     4441 tttttttaca gtcagcacaa agttttcgcg ggtattgatg gctatcacat atttccccgt
     4501 gagcgaatcc accggtattt tggtgatttt tttggtttcc acatttttta tctctatacg
     4561 cgccgtgaga ggctgctcgg aggtttcatc ctttacgtcg cccttcacca gtttcatttc
     4621 ttcgggacgg gcatcggcat agagatcaaa ctcatagaca tcccagccac ccacgcccac
     4681 cagcttggta gaagagaaaa aagccgtttt gccgtcgagg ctcacgaaaa aaccggcatc
     4741 gtcggcagcg gagtttatcg gataaccgag gttggcaggc ttcttccaac tgccatcggc
     4801 ctgaatacgc gagtagtaga tgtcaaagcc gcccagtccc atcaggtcgg gcgaatccga
     4861 cgaaaagtac aaggtctggc tgtcggtatg cagaaaaggt gttttttcat tgtttttggt
     4921 gttaatctga ggcccaaggt tctcgggttt tccccaaata ccatcctctc cttttaccga
     4981 acgatataag tcgaggccgc ctatgccccc gggtctgtca ctgacaaaat acagtgtctt
     5041 gccatcggaa gaaatgctcg gcatactttc ccaggtgtcg gggttattga tgttttcgac
     5101 aggcgctatc tcgctccagg tgccggattc gtcttttctg gaataataga tgtcgccgtt
     5161 gaagtaggag cctttatact ttcgtactga gtagtatagc tcgttattgt cgatagtcag
     5221 cgttgcgcct ccttcataat cgttcacgtt gaacgggtat ggcatttctt tgccattgtc
     5281 gtattgcccg tttttctttt cgctgtaata aaacttttct ttatataccg gtttgggcac
     5341 caggtcgttt ttgttgggcg gcaattctat cacccgcaca taaaacatat attctccgtc
     5401 gggcgagatg gagggtatgt attcatccga aacggacgac acgcccttga tatatctggg
     5461 attaaaaggc accgggttgc tgtaggcctt attataaaac accgcccatt tgtacatggc
     5521 cgatgcatcg ttcaactctg catcgttttc aatcttttcg ggatctttga ggaacttttt
     5581 taaagcattt tcggcttgtt cccatttttc ggcaccataa tatatttgcc ccaggtaata
     5641 gtacacgtaa acatcaaaat cagggcagag ttccacggat ttttcaaagt aattctgtgc
     5701 tgctgtcagg ttgtaatcag cttttttgaa gttgatcatg cccaggaaaa agtaacattg
     5761 tccgcattgt ggttcatatt cgagtacttc tttaaactgg gctgaagcat caataaaacg
     5821 gcggtttctg aaattttcaa aggcttcatt gtacaccttt ttggcttttt tgttggccag
     5881 ttcggtacac gggttttcgt cctgagcaaa tgaaaacatg ccgaggttca gcaaaataag
     5941 caggagcaga atatttttat tgtttctcgg catagacctg ttgcttaaaa agactatcgg
     6001 taactaccag ggcggtcatg gcttcaacta ccggtatggc gcgtggaaca aaacatacat
     6061 catgccttcc accggttgaa aactcaacgg tttgtgcctg acggttcacc gttttttgct
     6121 ttatttttat cccgggcacg ggtttaaaag ctacggaaaa atagatgtct tctccgttgc
     6181 ttatcccgcc ctgtattccg cccgagttgt tggtgg
//
LOCUS       DAOSFP010000109         6189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11150_length_6189_cov_4.277633, whole genome shotgun sequence.
ACCESSION   DAOSFP010000109 DAOSFP010000000
VERSION     DAOSFP010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11150_length_6189_cov_4.277633"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..960)
                     /locus_tag="PLI16_04415"
     CDS             complement(286..960)
                     /locus_tag="PLI16_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOH83833.1"
                     /translation="MQKCEHCTTKCGAASSLSPEQLEVMENNSVEVHFKKGDNIFRQG
                     ALSLNIAFLKNGIAKIHMHGPVREKILRIVRAPSYLGLPTSLGDKINQFSATALADTS
                     VCFIDNNLFRKLIYNNGQFAYEIIVELCRNELNDYQRYTSQSQKQIPGLVAETLLCMS
                     EKIFENSQFPFPLTQNELGDLVGTSRESISRVLSDFSTNKIIEFTGKQFTILDKETLN
                     QISEKG"
     gene            1106..1651
                     /locus_tag="PLI16_04420"
     CDS             1106..1651
                     /locus_tag="PLI16_04420"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOH83834.1"
                     /translation="MSNELEQQILDLQKKIGKIEKGTKDQLSMVVFSGDLDKILAAMI
                     ISTGAAAYDMKVKLFFTFWATAALRDPRKSVTGKTFLGKMFGMMLPKGMNKIKLSKMH
                     MMGMGTSMIKDLMKKKKVASLPEMFKTAGELGVEINICEMSMDLMGFKREEMIDYPQM
                     KVCGVATFLADAQESKVQLFI"
     gene            1797..2048
                     /locus_tag="PLI16_04425"
     CDS             1797..2048
                     /locus_tag="PLI16_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOH83835.1"
                     /translation="MTTEELKNLNVAQVVDARGTACPGPLLAAKKAIGEIESGEIMEV
                     LSSDEGTKHDIPRWCEKMEHEFLGIIEEDSYSRLFLKKA"
     gene            2313..2747
                     /locus_tag="PLI16_04430"
     CDS             2313..2747
                     /locus_tag="PLI16_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOH83836.1"
                     /translation="MADETYRPKILVFSTDKISDPGIDQAGLRKIHYSPSVYVISMPC
                     SSGIKPKWIMHALEKGFDGVFIAADGHECSYSPKCAEHTNKIITDAQALMKEKNISPK
                     RIRMAAICSVCAEPFASHMDNFSRILNDLGPVKDELANTTNQ"
     gene            2752..4083
                     /locus_tag="PLI16_04435"
     CDS             2752..4083
                     /locus_tag="PLI16_04435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH83837.1"
                     /translation="MQDEQKIFDTLVIGGGIAGQETALSLAEMDYQVLLVEKDLSIGG
                     KMIQLSKVFPTLDCAACITTPKMSETARHHNITLKLNTIIDDIRKVKDGYFEVSINEK
                     PRYVDPSLCTGCQECEFVCPQVINDHYNHNMVGRKAIYIPFNLANPKIAAIDIENCIH
                     CMRCVRACAPKAINFKEEERKTTVKAKSVVVCTGFRLFPGAGKPDYGYGQYPNIVDSL
                     QMDRLIAPTRPFNSLVRPGDGKAPENIAYVLCAGSRDSGIENKECAVGCFNNPICSQI
                     CCMYSIKQAQLLMGALPMADITIYYIDIRAFGKGYEEFFQQSNGMGVNFVKGKVAKIK
                     ENSNGSGDLVLQYEDVHSGVYKEAKHDMVVLSVGVLPEDNVTGLFSNEKLELDDFKFV
                     KQTDPFLNPALTSIAGVFVAGMASGPKDIPDSILSAGCAATEVAGYLAKIK"
     gene            4251..4628
                     /locus_tag="PLI16_04440"
     CDS             4251..4628
                     /locus_tag="PLI16_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOH83838.1"
                     /translation="MTENELSKIIVNTAYQIHHKLGPGLLESVYEEIMYYELSKQGVN
                     VERQKGIPVIWDELRMEIGFRADLIVEKKVIIELKSVEAIAPVHPKQLLTYLKLTGLK
                     LGLLINFNEPLIKNGITRIVNNL"
     gene            4686..>6189
                     /locus_tag="PLI16_04445"
     CDS             4686..>6189
                     /locus_tag="PLI16_04445"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOH83839.1"
                     /translation="MNKRIGVYICHCGGNISDYVDVAEAQRLMQDEDGVLISKHVMFA
                     CSDATQTEMVNDIKENQLDAIVVASCSPKLHTHTFRGVADRAGLNKYNYIQVNIREQC
                     SWPHSDKPLDATHKAIGLIRAGIKKAALSEALESVEIEAYDAMMVVGAGVSGLKAALE
                     LAKNGHQVYLLEKEKKVGGKLVENGSLFMTGEHGNQLVERLLEEIKNNTKITLFTESE
                     VEKVNGSIGNFNIDVNVGMGGVKTEKMTLAVGSVLVATGYDYYQPKENEFAFGMSPRV
                     ITLPELKKRMEKNNGVIIHNNKPVKSLAFVYCVGNRQRKGENKYCSRICCTTAIHTGL
                     LLKEKNKDLKIIHLYRDIRTYGKQELLYEQSSKQGDVYMKFDEKEPPQVDVKGDSLLV
                     KVKDYLTQKKEIEFSPDLLVLVTGAVARSDSSDIAAKFKIPVGADRFFNEIHPKLKPV
                     ETVINGVLIGGACQSPKNVSESVQSSLSAVSKMLSLIKNNRIRLEPIIARV"
BASE COUNT         1993 a         1213 c         1355 g         1628 t
ORIGIN      
        1 gcaattacaa caatacggta gaaaacgttt gtagcaagga agacaaagat gaactttcgg
       61 gcaagtttta cgaatgcctc gaagaataga ttgtttttac ctcagagtgg ttgcactata
      121 attttttgca atgatttgaa gattcctcat cctgcgaaag tagcgggatt tggaatgaca
      181 tgatgattag tttactccct ccccgatgcg tcatacaaag ctatcatcct gagcggagtg
      241 caacgtagcg aaggatctca aaaataaata cggtttccct tcaatttacc ctttttcgct
      301 gatctggttt aaagtttctt tatccagtat ggtaaactgt ttccctgtaa attcaatgat
      361 tttgtttgtg ctgaaatccg acagtaccct gctgatactc tcgcgcgagg tgcccacaag
      421 gtctcctaac tcattctgag tcagcggaaa cggaaattgc gagttctcaa atatcttttc
      481 tgacatacaa agcagggttt cagccaccag gccgggaatc tgtttttgcg attggcttgt
      541 ataacgctgg taatcgttta gttcgttccg gcataattcc acaatgattt cataggcaaa
      601 ttggccattg ttataaataa gctttctgaa aaggttgttg tcaataaagc aaaccgaggt
      661 atctgccagt gcggtggccg aaaattgatt gattttgtca cccagactgg tcggaagacc
      721 taaataggaa ggcgccctga caatacgaag gattttttca cgtaccggcc cgtgcatgtg
      781 tattttagcg ataccgtttt tcaggaaagc tatgttgagc gacaaagctc cctgcctgaa
      841 aatattatct ccttttttga aatgtacctc cacactattg ttttccatta cctccaattg
      901 ctcgggcgac aggctgcttg ccgctccgca ttttgtggtg caatgttcac atttttgcat
      961 gatcattggt tttaaatgct taaagataat aaaaaatcgc actatgtgat ttaaatcaca
     1021 ttattttttg caaaacctca catgcaattt tgtcaaatca caaacaaaca taactatctt
     1081 tgttacaaac caaaaacaaa aaaatatgag taacgaatta gaacaacaaa tacttgattt
     1141 acaaaaaaaa atcgggaaaa ttgaaaaagg cacgaaggat caactttcta tggttgtttt
     1201 ctcgggcgat ctggacaaaa ttctggcagc catgattatc tccacaggag cggctgctta
     1261 tgatatgaag gtaaaactat tttttacttt ctgggctact gccgcgttgc gcgatcccag
     1321 gaaatcagta accggcaaga cttttttagg caagatgttt ggcatgatgt tgcccaaagg
     1381 aatgaacaaa ataaaattgt caaaaatgca catgatgggc atgggtactt ctatgatcaa
     1441 agacttgatg aaaaagaaaa aggtagcatc cctgcccgag atgttcaaaa ccgccggtga
     1501 attgggcgtg gaaataaata tttgcgaaat gtcgatggac ctgatgggtt tcaaacgcga
     1561 agaaatgata gactacccgc aaatgaaggt ttgtggcgtg gccactttcc ttgccgatgc
     1621 ccaggaaagt aaagtgcagt tatttattta attactaatt aatcgcaaag tacgctaagg
     1681 aatgtacgaa tgaaaatcac ttgccgaacc ctttgatagg gaacaatatc tatttcatat
     1741 cttagaactt ctttgcagta aaaacaaaaa ttattaactc ataaaaaata aaaatcatga
     1801 caacagaaga attaaaaaat ttgaatgtag cacaagtagt tgacgcacgc ggaaccgcct
     1861 gcccgggacc tttattggca gcaaagaagg ctattggcga aatcgagtcc ggcgaaatca
     1921 tggaagtttt gtcttcagat gaaggaacaa aacatgatat tccgaggtgg tgcgaaaaaa
     1981 tggagcacga atttctggga ataatagaag aagacagcta ttcgaggctg tttttgaaga
     2041 aagcgtagca agggacaagt aacaagtggc aagtagaaag taacaagtgg caagttgcaa
     2101 gttgcacaaa acaacgaact ttccactttc aactttccac taataactat ttgactaatg
     2161 accagtgact tgtgctcaat gaccagtgac cagtgacgaa tgaccactga ctacttcctt
     2221 cttcccactt tcaactttca actttccact atttgactaa tgactaatga ccattgacca
     2281 ttgactaatt atttatcctt aatttaattt gtatggcaga cgaaacgtac cgaccgaaaa
     2341 tacttgtgtt ttcaaccgac aaaatctccg acccgggcat cgaccaggca ggtttgcgca
     2401 agattcatta ttcaccttca gtgtatgtga tcagcatgcc gtgttcttca ggtatcaagc
     2461 caaaatggat catgcatgcc ctggaaaaag gttttgatgg cgtatttatc gcagccgacg
     2521 ggcacgaatg ttcttacagc cctaagtgtg ccgagcatac caataagatc atcaccgatg
     2581 cacaggcact gatgaaagag aaaaatatca gtccaaaacg tatccgcatg gccgccatct
     2641 gttcggtatg tgccgaacca tttgccagcc acatggacaa tttctcccga atacttaatg
     2701 atttgggtcc cgttaaagat gaattagcca acacaactaa tcaataatat tatgcaggac
     2761 gaacaaaaaa tattcgacac cctggtcata ggtggaggta ttgccggcca ggaaacggct
     2821 ctcagcctgg ccgaaatgga ttatcaggta ttgcttgtcg aaaaagacct ttcaattggt
     2881 ggtaaaatga ttcaactgag taaggttttt cccacactcg attgtgctgc ctgcattact
     2941 actcccaaaa tgtcggaaac agccagacac cataatatta ccctcaaact aaacaccatt
     3001 attgatgata tccgaaaagt caaggatggc tattttgaag tcagcataaa cgaaaagccc
     3061 agatatgtcg acccctcgct atgtaccggc tgtcaggaat gtgagtttgt ttgtccgcag
     3121 gtaatcaacg accactacaa ccacaatatg gtgggacgaa aagctatata cattcctttt
     3181 aatttggcta accccaaaat tgctgccatt gatattgaaa actgcataca ttgtatgcgc
     3241 tgtgttaggg cctgtgctcc gaaagccatt aacttcaagg aagaagaacg taagactacc
     3301 gtcaaggcaa agtcggtggt ggtttgtacc ggttttcgct tgttcccggg ggcaggtaag
     3361 cccgattacg gctatggtca atacccaaat attgtggatt ccctccagat ggatcgcctt
     3421 atagccccca caaggccttt caatagtttg gtgaggcctg gcgacggcaa agccccggag
     3481 aatatcgctt atgtgttgtg tgccggctca cgcgattcgg gcatagaaaa caaagaatgt
     3541 gctgttggat gctttaacaa cccgatatgt tcacagattt gctgcatgta ttccattaaa
     3601 caggcacaac tgcttatggg tgctttgcct atggccgata tcacaatcta ctatattgat
     3661 attcgggctt ttggaaaagg gtacgaagaa ttttttcagc aatccaatgg catgggtgtg
     3721 aattttgtca aaggaaaagt agccaaaatt aaagaaaata gcaacggaag cggtgacctt
     3781 gtgctacaat acgaggacgt tcatagcggc gtctacaaag aagccaaaca tgatatggtg
     3841 gttttgtccg ttggtgtgct tcccgaagat aatgttaccg gcttgtttag caatgaaaaa
     3901 ctggaactcg atgattttaa atttgttaaa caaacagatc cttttttaaa ccctgccctc
     3961 accagtattg ctggagtgtt tgtggccggc atggcttcgg gtccaaaaga tatccccgat
     4021 tcgatattat ctgccggttg cgctgctaca gaggtggcgg gttatttagc aaaaataaaa
     4081 taatataaaa atttatctcg caaagacgga aaggcgcaaa gaaaaaagac ggaaaggcgc
     4141 aaaggaaaaa gacggaaagg cgcaaaggaa aaagacggaa aggcgcaaag gaaaaagaca
     4201 aaaagacaca aaaaaaatac gcaaaggagc aaaaaaaaga ataattaggt atgacagaaa
     4261 acgaattatc aaaaattatt gtaaacactg catatcagat tcatcataaa cttggcccgg
     4321 gtttactgga atcggtttat gaagaaataa tgtattatga attgagtaaa cagggggtga
     4381 atgttgaacg acaaaaaggg atacctgtaa tttgggatga attgagaatg gaaattggtt
     4441 ttagagcaga tttaattgta gagaaaaaag taatcattga attaaaatca gttgaagcga
     4501 ttgccccggt tcatccaaaa caattgctga cttatttaaa attaaccggt cttaaactgg
     4561 gattactaat taatttcaat gaacctttaa taaaaaatgg aattaccaga attgtaaata
     4621 atttataaaa gctatgcgcc tttgcgcctt tgcgagaaat aaaaaaacat ttgaaaatta
     4681 taaatatgaa caaaagaata ggagtatata tctgccattg tggaggtaac atttccgatt
     4741 atgtagatgt ggctgaagct caacggctga tgcaggatga agatggtgtc cttatctcta
     4801 agcatgtgat gtttgcttgt tcggatgcta ctcaaaccga aatggtgaac gacattaagg
     4861 agaaccagtt ggatgccatt gtggtagctt cctgttcacc caaattgcat acacacacat
     4921 tcagaggtgt ggccgaccgt gcaggtctta acaagtataa ctacatacag gtaaatattc
     4981 gtgaacaatg ttcatggcca cattccgaca aacccctcga tgctactcac aaagccattg
     5041 gcctgatacg tgctggtata aaaaaggcgg ccctgtccga agccctcgaa agtgttgaga
     5101 ttgaggctta tgatgctatg atggttgttg gcgcaggcgt gtcggggttg aaagcggccc
     5161 ttgagttggc aaaaaacggc catcaggttt atctgttaga aaaagaaaaa aaagtgggag
     5221 ggaaactggt ggaaaacggc tcgctattca tgacaggaga gcatggaaat caacttgttg
     5281 aacgcctgct ggaagaaata aaaaacaaca caaaaattac actctttacc gaatcagaag
     5341 tagaaaaagt taacggcagt atcggaaatt tcaacattga tgttaatgtg ggcatgggag
     5401 gcgtcaaaac tgaaaaaatg acgctggcag ttggctcggt gctggtagcc acgggatatg
     5461 attattatca gcctaaagaa aacgaatttg cttttggcat gtcaccacgg gttattaccc
     5521 ttcccgaact gaaaaaacgc atggaaaaaa acaacggcgt gattatacat aacaacaagc
     5581 ctgtcaaatc tttggctttt gtttattgtg ttggaaaccg ccaacgcaaa ggcgaaaata
     5641 agtattgttc acgcatctgc tgtactaccg ccatacacac cggcctgctg ctcaaagaaa
     5701 agaataagga tttgaaaatc attcacttgt atcgcgatat ccgcacctat ggtaaacagg
     5761 aactgcttta tgaacaatca tcaaaacaag gtgatgtata catgaagttt gatgagaagg
     5821 agccgccaca agtggatgtg aagggcgaca gtttgctggt caaggtaaaa gattatctga
     5881 cacagaaaaa agaaatagaa ttcagccccg acctgttggt gttggttacc ggagctgtgg
     5941 cacgttccga cagcagcgac atagccgcta aattcaaaat tccggtaggc gccgacaggt
     6001 ttttcaatga aatacatccc aaactaaaac ccgtggaaac cgtgataaac ggtgtgttga
     6061 taggaggtgc ctgccagagc ccaaaaaatg tcagtgaatc agtacagtca tccttgtcgg
     6121 cagtttccaa aatgttgtcc ctgataaaga acaatcgcat tcgtcttgag ccaatcattg
     6181 caagggtca
//
LOCUS       DAOSFP010000110         6161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11217_length_6161_cov_3.521291, whole genome shotgun sequence.
ACCESSION   DAOSFP010000110 DAOSFP010000000
VERSION     DAOSFP010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11217_length_6161_cov_3.521291"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLI16_04450"
     CDS             complement(<1..557)
                     /locus_tag="PLI16_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOH83840.1"
                     /translation="MKKIFLIALALVGGLLCQAQGKKEIILPVANTEGGKPFMETLKD
                     RHTTRSFSEEKLSDQQLSNLLWAAFGINRPESGKRTAPSAMNNQETDIYVCLSSGVFL
                     YDAKKHSLILVVEEDYRKNMGRQDFVASAPVILLFVADYSKMGEKVSAEKKDFYSAVD
                     VGYISQNVYLYCASENLGTVVLGSV"
     gene            complement(566..1153)
                     /gene="coaE"
                     /locus_tag="PLI16_04455"
     CDS             complement(566..1153)
                     /gene="coaE"
                     /locus_tag="PLI16_04455"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.2"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOH83841.1"
                     /translation="MIRLGLTGGIGTGKSMVADIFSHLDIPIFYGDQEAKKAYADPEI
                     LLKVKQVFGNTVFDNDKVNYRKLAAVVFPHPEMLEKLNSIIHPFLISRFERWSGQQHR
                     APYIIMEAAILFEASLQQFFDATVAVSAPRELCIERVMNRDGLQRDEVIRRMDNQWPD
                     AQKTAMANYVVINDGEHLLIPQVLAIHQSVLNSIK"
     gene            complement(1150..2154)
                     /locus_tag="PLI16_04460"
     CDS             complement(1150..2154)
                     /locus_tag="PLI16_04460"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOH83842.1"
                     /translation="MTSGSYDISLDEQKDNQKRSKRKLNIFIICFVIASISWLMIKLS
                     GTYSHNVIVSITYKGIKNNRIMLPSSDTVLIANIQSSGYNIIYNRLLNEDYRFQLDLS
                     QYPARRNGDIYEINIETANLNYRLGNYFKTGEKIVSIYPANMRVRLERAYTKKVPVKA
                     NVDITYKKQYAGYKHIYLEPDSVYITGSKAVIDKISFVETEKNEQKNLSKNTMLELSL
                     KNPLNKFLLRYSADKVKMYIPVAEYTEEYVDIPLTFETVPNGYQLIAYPDKIRCYFNV
                     SIPDYDKISPEAFKAGINEKSLFNSKHNTAKVVLKKVPRYARVQRIEPESIEYLIRKK
                     "
     gene            complement(2191..2514)
                     /gene="yajC"
                     /locus_tag="PLI16_04465"
     CDS             complement(2191..2514)
                     /gene="yajC"
                     /locus_tag="PLI16_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOH83843.1"
                     /translation="MNLLSVFLMMGQQGQQGSGSMVSTIVMMVLIVVVFWLFFIRPQS
                     KKNKELQKFREGLKRGDKVITAGGIHGKILEVLDTTVIIETEGLGKLKVEKSSIASSV
                     AGNGE"
     gene            complement(2526..2981)
                     /locus_tag="PLI16_04470"
     CDS             complement(2526..2981)
                     /locus_tag="PLI16_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH83844.1"
                     /translation="MRRPVYIFFIASLLFFSCGNNTDTLNTDLVNNPNTADGKADMSG
                     LPAFDFVTEEHDFGRITDGEKVSFTFKFKNSGKSDLIISEAKATCGCTVADYPRTPIK
                     PGAESGISVSFNSEGRKGIQHKTVTLVANTQPSTKVLTIKAEVVNLGIN"
     gene            complement(3027..3956)
                     /gene="nusB"
                     /locus_tag="PLI16_04475"
     CDS             complement(3027..3956)
                     /gene="nusB"
                     /locus_tag="PLI16_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOH83845.1"
                     /translation="MQALYAFFQSDNDRVDVAVRNLQKNIYGIYNLYIYQMSFLIEIV
                     DFARETIEIKKNKYFPTEEEANPNTRFVNNSIILKIENSPDFKRKHEELKINWADQEV
                     LIRKTYQQITEADFFQAYMKNQEDSSDNDFRVLEQIVDTFIAENEALQQYFEDINVFW
                     ADDFDVACFMLVKSLKYLKNSTEKNPFLPTLFGNQQGDDEKEEIKFLTDLFSKTVMHR
                     DEFDDLIEARVDNWEIDRIALMDSLLIKMALAEFTEFPTIPTKVTINEYIEISKMYST
                     PKSKIFINGLLDKLLFELTEKKKIKKTGRGLIG"
     gene            4149..4514
                     /locus_tag="PLI16_04480"
     CDS             4149..4514
                     /locus_tag="PLI16_04480"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOH83846.1"
                     /translation="MEEKDFNERNDREEIFSKAVRAGKRTYFFDVKSTRSEDYYLTIT
                     ESKRRFSNESGKFFYEKHKLFLYREDFEKFLKGLDVVMKFIETGEVPAPDPEDDQLAA
                     EEYQENKENKTEEDSEGYK"
     gene            4664..5479
                     /locus_tag="PLI16_04485"
     CDS             4664..5479
                     /locus_tag="PLI16_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH83847.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLSASLAVLEFKTLLIDADPQAN
                     ATSGVGFDPKNIKTSIYECIIEDVEPKNIILNTKTPNLDLLPAHIDLVGAEIELINQP
                     HREKMMRKVISKIKDNYDFIIIDCSPSLGLIVVNSLAAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQQHMNPDLDIEGILLTMYDQRLRLSNQVVEEVKTHFQQMVFNTIIQRNTK
                     LGEAPSFGETIIMYDADSKGAINYLNLAREILQKNELTRIKQSDKIINTEDDK"
     gene            5469..>6161
                     /locus_tag="PLI16_04490"
     CDS             5469..>6161
                     /locus_tag="PLI16_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOH83848.1"
                     /translation="MTNKKRSTVIGRGLSSLLENPDTDITSKSDVMGKYVVGSVAEIS
                     VSQIEANPFQPRDQFDEIALEELAESIKTHGLIQPVTVRKLGFDKYQLITGERRFRAA
                     QIAGLKEIPAYIRIADDEQMLEWALIENIHREDLNPIEIALSYQRLVDECKLTQAELS
                     EKVNSKRSTVTNYLRLLKLPEIIQKALKEEQLSMGHARAIAGIEEAERQLRIFNMIVD
                     QGLSVREAEKLTK"
BASE COUNT         1747 a         1278 c         1290 g         1846 t
ORIGIN      
        1 tcaactgatc ccagcactac ggtgcccagg ttttcggaag cgcaatacag gtaaacgttc
       61 tggctgatat agcccacatc tactgccgaa taaaaatcct ttttttctgc ggaaaccttt
      121 tcccccattt ttgaataatc tgccacaaat aacaaaatta ctggcgcact ggctacaaaa
      181 tcctgcctgc ccatattttt acggtagtct tcttcaacta ccaaaatcag gctgtgcttt
      241 ttggcatcgt aaagaaacac accggaactc aggcagacat agatgtcggt ttcctgatta
      301 ttcatagccg aaggcgccgt gcgttttccc gattcggggc ggttgatgcc aaaggcagcc
      361 cataagagat tggaaagttg ttgatccgat agtttttcct cgctaaaact ccgggttgta
      421 tgcctgtctt ttaaggtttc cataaaaggt ttgcctcctt cggtattggc aactggcagt
      481 attatttctt tttttccctg tgcctggcat aacagacctc ctaccagcgc taatgcaatt
      541 aaaaagattt ttttcataac attttttatt ttatagagtt aagtactgat tgatgaatgg
      601 cgagcacctg cggaatgagc aggtgttcgc catcatttat aacgacataa ttagccatgg
      661 ctgtcttttg ggcatcaggc cattggttat ccatgcgccg aatcacctca tccctttgca
      721 ggccgtcgcg gttcattacc ctttcaatgc aaagttcgcg gggagcgctg acggcaaccg
      781 tggcgtcaaa aaattgctgc agcgaagctt caaaaagtat agctgcttcc attatgatgt
      841 aaggtgcacg atgctgctgg cccgaccatc tttcgaaccg gctgataaga aaaggatgaa
      901 ttattgaatt taacttttcg agcatctccg ggtggggaaa aaccacggcg gcaagttttc
      961 tatagtttac cttgtcgttg tcgaaaactg tgttgccgaa gacctgtttg actttaagca
     1021 atatttccgg gtcagcataa gccttttttg cttcctgatc gccataaaag atggggatgt
     1081 caagatgaga aaatatatcg gccaccatgg atttgccggt gccaatgccc ccagtaagtc
     1141 ccaacctgat cattttttac gtattaaata ttcaatactt tcgggttcaa tacgctgcac
     1201 cctggcgtag cggggaactt ttttcaggac gacctttgcc gtgttatgct tggagttgaa
     1261 caggcttttt tcgttgatgc ctgccttgaa tgcttcagga gaaattttat cgtaatcggg
     1321 gatgcttaca ttaaaatagc atctgatttt atcgggatag gcaatcagct gatagccatt
     1381 cggcaccgtt tcgaaggtaa gaggaatgtc cacgtactct tcggtatatt cagccacagg
     1441 aatgtacatt tttaccttgt cggccgaata tctcaataaa aatttgttta acgggttttt
     1501 gagcgataac tccagcatgg tatttttgga caaattcttt tgttcgtttt tttcggtttc
     1561 aacaaaagaa attttatcaa tcacagcctt gcttccggta atatacaccg agtcaggttc
     1621 caggtaaata tgcttgtacc cggcatattg ttttttatag gtgatatcca cattggcttt
     1681 taccggaact tttttggtat aggccctttc gagccgcacg cgcatattgg caggataaat
     1741 ggaaactatt ttttcgcctg ttttaaagta atttccaagc cggtaattga ggttggcggt
     1801 ttcgatattt atttcataaa tatcaccatt tctacgtgcg ggatattggc tcagatccag
     1861 ttgaaatctg tagtcttcgt tcagcaggcg gttataaatg atattgtaac ccgatgattg
     1921 gatgttggcg atgagtacag tatcagacga aggcaacata atccggttat ttttgatgcc
     1981 tttgtaagta atagatacta tgacattatg ggagtatgtt cccgaaagct ttatcatcag
     2041 ccagcttatg gaggcaataa caaagcagat aataaaaata ttcagttttc tttttgaacg
     2101 cttttgatta tctttttgtt catcaagaga tatatcataa cttcctgatg tcaaggccgg
     2161 aaatttatta ggttatattt tccgtgtttt ttattcaccg tttccggcaa ctgaagaggc
     2221 aattgaactt ttttcaacct taagttttcc taaaccttct gtttcgatga tcactgtggt
     2281 gtccagcact tcgaggattt ttccgtggat accgccggcg gtaataactt tatcgccacg
     2341 tttgaggcct tctctgaact tttgcagttc cttattcttt tttgattgcg gacggataaa
     2401 aaacaaccag aaaactacca cgatcaaaac catcataacg atggtgctaa ccatactgcc
     2461 acttccttgc tggccttgct ggcccatcat taaaaaaaca cttaataaat tcattttgtt
     2521 ttcgtttaat ttatacctaa atttactact tctgctttaa tagttaatac tttggtgcta
     2581 ggctgggtgt tagccaccag ggttactgtt ttatgctgaa ttcctttgcg gccttcgcta
     2641 ttaaaagaaa ccgagatgcc gctttcggcg ccgggtttta tgggagtccg ggggtaatcg
     2701 gctactgtgc acccgcaggt agcttttgct tcggagatga tcaggtcgga ttttcctgag
     2761 tttttaaatt taaaagtaaa acttactttt tccccatcgg ttatccggcc aaagtcgtgt
     2821 tcttcggtaa caaaatcaaa agcaggcagg ccactcatgt ccgccttgcc gtcggccgtg
     2881 tttggattgt tgaccaggtc ggtgttgagg gtgtcggtgt tgttaccgca tgaaaaaaac
     2941 agcagggatg caataaaaaa aatataaacc gggcgacgca taaaactaat tttttacaaa
     3001 aatacaaaaa ttatgaaaga aaaaatttat cctatcaatc ccctgcctgt ttttttgatc
     3061 tttttctttt ctgtcaattc gaataagagc ttatcaagca acccgtttat gaatattttg
     3121 cttttggggg tgctgtacat tttcgatatc tcgatatact cgttgatggt tacttttgta
     3181 gggatggtag gaaattcggt aaattcggcc agggccattt taatcagcag cgagtccatc
     3241 agggcgatgc ggtcaatctc ccagttatct acgcgggctt ctatcaaatc gtcaaactca
     3301 tcgcggtgca tgactgtttt tgaaaaaagg tcggtaagaa attttatttc ttccttctcg
     3361 tcatcgcctt gctggttgcc aaagagcgtt ggcaggaacg gatttttttc agtagagttt
     3421 ttgaggtatt tcagggattt taccaacata aaacaagcca catcaaaatc atcagcccaa
     3481 aatacattta tatcttcaaa atactgctgc agtgcctcgt tttcggcaat aaaagtatcc
     3541 actatctgtt ccaggaccct gaaatcattg tcggaggaat cctcctggtt tttcatatat
     3601 gcctggaaaa agtccgcttc ggttatttgt tggtaagtct tgcggatcag cacttcctgg
     3661 tcagcccagt tgattttcag ttcttcgtgt tttcttttaa aatcaggact gttttcgatt
     3721 tttaaaatga tgctgttgtt gacaaaacgg gtatttgggt tggcttcttc ctcggtggga
     3781 aaatatttgt ttttcttgat ttcaatggtt tcgcgtgcga aatctacaat ttcaatcaaa
     3841 aaagacattt ggtaaatata gaggttgtaa ataccgtaaa tatttttttg caggtttctt
     3901 acagcaacat ccacgcggtc gttatcagac tgaaagaacg cataaagggc ttgcatcacc
     3961 tttattctta gtactcgtcg gtttaacatt tatctgaagg ctcaaatttt gcacgaaagt
     4021 acacaaaaat attttttatg tcagtttttt tttcaggtga acaaccattg ccgtaaaaat
     4081 tttgtcataa atactaattg aaatattacg gaaagttttt atttttgtaa caataaaaca
     4141 aggactatat ggaagagaaa gattttaatg aaagaaacga ccgtgaagag attttctcta
     4201 aagcagtaag ggcagggaaa agaacctatt tttttgatgt aaaatcaacg cggagcgaag
     4261 actattacct gacgattact gaaagcaaaa gacgtttcag caacgagtcg ggaaagttct
     4321 tctacgaaaa gcacaaactt tttttgtatc gtgaagactt tgaaaaattc ctcaaaggtc
     4381 tcgacgtggt gatgaaattt attgaaaccg gtgaagtgcc agcccccgat cccgaagatg
     4441 atcaactggc agccgaagaa tatcaggaaa acaaagaaaa caaaactgag gaagattctg
     4501 agggttataa ataaaccttt tcttcatttc atattttttg ttaaaataaa ccacaatatt
     4561 ctggtttata atgttttgtg ttttcacttt tagtgcaaaa ctttatttat taattcggat
     4621 aaaaggtggt atttttgtaa tctgaaaaaa tcgaaagttt tctatgggaa aagtcattgc
     4681 tattgctaac caaaaaggag gagtaggaaa aaccactaca gccatcaatc ttagcgccag
     4741 cctcgctgtg cttgagttca agacattgct gattgatgcc gacccgcagg ccaatgccac
     4801 ttccggagtc gggtttgacc caaagaatat caaaaccagt atttatgagt gtatcatcga
     4861 agatgtagag cccaaaaata tcatcctgaa cacgaaaaca cccaacctgg acctgctgcc
     4921 tgcccacatc gacctggtgg gcgccgagat tgaactgatt aaccaaccgc accgcgaaaa
     4981 aatgatgcgc aaggtcatca gcaaaataaa ggacaattac gattttatca tcatcgactg
     5041 ttccccttct ctcggcctga tcgtggtcaa ctcgctggcg gcggccgatt cggtcatcat
     5101 ccccgttcaa tgcgaatatt ttgctctgga aggactcgga aaactgctga ataccataaa
     5161 aattgtgcag caacacatga acccggacct ggacattgag ggcattttgc tcaccatgta
     5221 cgaccagcgt ctgcgcctgt ccaatcaggt ggttgaagaa gtaaaaacgc attttcaaca
     5281 aatggtgttc aacaccatta tccagcgcaa taccaaactt ggtgaagccc ccagctttgg
     5341 cgaaactatc atcatgtatg acgccgacag caagggagct atcaactacc tgaacttggc
     5401 cagggaaatt ttgcagaaaa acgaattaac caggataaaa caaagcgata aaataatcaa
     5461 taccgaagat gacaaataag aaaagaagca ctgttattgg ccgaggcctc agttccctgc
     5521 tcgaaaaccc cgataccgat attaccagca aaagcgatgt gatgggaaaa tatgtggtgg
     5581 gttcggtagc cgaaatcagc gtatcgcaga ttgaagctaa tccttttcag ccccgcgacc
     5641 agtttgatga gattgccctg gaagaattag ctgaatccat taaaactcac ggactcattc
     5701 agccggttac agtgcgcaaa ctgggttttg ataaatacca gcttattacc ggcgaacgcc
     5761 gtttcagagc cgcacaaata gcagggttga aagaaatacc tgcctatata cgcattgctg
     5821 atgacgaaca gatgctggaa tgggcactta tcgaaaatat acaccgtgaa gaccttaacc
     5881 ctatcgaaat tgctctttcg taccagcgac tggtggatga atgcaaactg actcaggccg
     5941 aattaagcga aaaagttaac tccaaacgtt ctaccgttac taattatctg cggctcctta
     6001 aactgcccga aattattcag aaagccctga aggaagaaca actcagcatg ggccatgccc
     6061 gcgccattgc gggtattgaa gaggctgaaa gacaattgcg catttttaac atgattgttg
     6121 atcagggtct ttcggtgcgc gaagctgaaa aacttaccaa a
//
LOCUS       DAOSFP010000111         6155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11230_length_6155_cov_3.803934, whole genome shotgun sequence.
ACCESSION   DAOSFP010000111 DAOSFP010000000
VERSION     DAOSFP010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11230_length_6155_cov_3.803934"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="PLI16_04495"
     CDS             complement(<1..1339)
                     /locus_tag="PLI16_04495"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOH83849.1"
                     /translation="MEKKYTLIIVFLITIGLSLKTEQHAQAQLAVAQGAAIGLTPLQL
                     VQQVLVGSGVTVSNATFNGSAALINSNQVGNFTTAGGATTQLGFTGGIILASGNAADA
                     IGPNSSGSSGVGTGSGSDPDLQSLVTSTIYDKAVLEFDFIPLSDTIKFRYVYGSEEFD
                     EFCNSSYNDAFGFFISGPGITGPFTGNAKNIAVMPNNPANYVTINNVCNAGATYSWLN
                     SGVYLQYDRLTYIYTAWCLVQPCQTYHIKLAIGDAGDSAFDSGVFLEENSFTSNAVSH
                     TTAFSSSVDTIAVEGCNNAVVTFHLANPATSPVTINHTIGGTAIEGVDYLDIPNNFVI
                     PTGQDSYTVTITPIADGIAEGTETVTITYTNSVCGTTDMVVILIRNNNPLSVTTTPDV
                     YSCSGLPVTISATVSGGITPPAYTYQWSVGAGNTASVVVDPATPTMYYVTVTDI"
     gene            complement(1788..3515)
                     /locus_tag="PLI16_04500"
     CDS             complement(1788..3515)
                     /locus_tag="PLI16_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83850.1"
                     /translation="MKHNNKMNKHIMKTPKYIVAVMLIAIVCGTLQAQKPYDEKVTVI
                     APYQPTISDANKINVQPVHPDTIFVTPRMSYQTLTRKYPTTYTTDPIKPARVGDINLA
                     KLYKFLVKAGFGNYTSPYGEFFVNNTYSRSYSVGGHLKHHSYLGKFKDWAYPGNSENL
                     AEFYGKKFFKQHELGGGIGYKRNVYHYYGFKPGDYPDIISVLPLDRDLYKQRFNLINA
                     DVYFKTIHHPDSLKLNYSAGFKYYFLNDYYKTSENAIDFTADINKDLHLFKITSSQFV
                     GLQAQVKYCFDQDSLYEKNAGLISLKPYIKTTFKGFNFNLGVDISISAANNAKLYFYP
                     IADVQFNIVKNILIVFAGIKGHLQQNNFYSTISENPYINSYIPLSYTSTTYDIFGGIK
                     SDISKTMNFSAAVSYSRIKNLPLYVTDTTLVFRNKFNVIHDSARLLNVRGEFVYHKDE
                     KFKVLVGGNFYHYKMDNELKAWHKPIFDAYVDFWYNIRDKFIITAGVTGRSHIYTKTF
                     KRTDLGIEAGKKTFKGYADVSLGFEYRYSKILSAFINVNNIANIKYYTWNNYRAYGIN
                     MMAGVTYAF"
     gene            complement(3528..4802)
                     /locus_tag="PLI16_04505"
     CDS             complement(3528..4802)
                     /locus_tag="PLI16_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83851.1"
                     /translation="MMSLIDKYPKSNYRVSAIYELANTYQNLNNNGKAIDYYDMLTRE
                     YPNSGYVSQALLKKGMIYYNEQKDEQALQVLKKVVSDFPGTPESKEALVSIRNVYVDM
                     DRVEDFFVYVKDLPFAAVSNAEQDSITYIALENRYMRNDCDNAMAGFKDYITKFPNGA
                     YLIDAHFYLAECQYRAKLTDEALQNYNFVIDKPRTKFTEKSLLNAAAINFSRKNYEAA
                     LTNYTDLEKYAEYKENILTAQTGQMRCTYLLNLYSTAISASQKLLLADKLPEDLMVEA
                     HLTIAKSALASKDTTLALTEFETVTKLSKSESCAEAKYSIAEIYYLQNLTEASEKVAF
                     DLINQVPSYDYWVAKAFILLADNYVKTGNRHQAKYTLKSIIDNYEGADLIKIAEEKLN
                     AILEAEKIEEQKKAEELLKIQNEPGPLLNEIK"
     gene            complement(4812..>6155)
                     /locus_tag="PLI16_04510"
     CDS             complement(4812..>6155)
                     /locus_tag="PLI16_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83852.1"
                     /translation="QDYANAKKSFFEIIDKESKYTAPANYYYAHIAYTEKNYESALKA
                     FSKLLNDENYSKIAPFYMIQIYYFQQKYDEIIALAPVLLDSAGNSKRTPEIARIAAEA
                     YYHTERYKEALPYLEMFLQKNTAPLLRKDHYMIAYTYYKAEEYGKAIENFKKISTAEN
                     DSLSQLACYHQAQCYLATGQKQFAMNMFASAYKMDADKQLQENAMFSYAKLAYETAFN
                     PYNEAVNVFNEYIEKYPESENLDEAYEYLSNIYLTTRNYKDALSSLEKIKKRDNKLNK
                     AYQKIAYLRGIELFNNNEYNEAIAMLAQSNKYPIITEYEIQTYYWTAEAYYRQQKFDS
                     AIYNYQLLQSTTGAFGNEPYLNSYYGLGYSYFKKASYTSALSNFKKFTAEAKNATPRI
                     INDAYNRTGDCYFMNKDFANAVENYDKSLQMNLVDADYALYQKALAQGASSKLET"
BASE COUNT         1559 a         1166 c         1510 g         1920 t
ORIGIN      
        1 atatatcggt aaccgtcaca taatacatgg taggcgtagc agggtcaacc accacggagg
       61 ccgtgtttcc ggcgcccacg ctccattgat aggtatatgc cggaggagtg atacctcctg
      121 aaacggtggc gctgatggtt accggtaacc cactgcagga atagacatcg ggagtggtgg
      181 taacgctgag cgggttattg ttgcgtatga ggataaccac catatcggtg gttccgcaaa
      241 cactgttggt ataggtaata gtaacagttt cagtaccttc agctatgccg tcggcaatgg
      301 gagtgatggt tacggtgtag ctatcctggc ctgtgggaat cacaaagttg tttggaatgt
      361 ccaggtaatc aactccttca atagccgtcc cgccaatggt atggttgatt gttaccggag
      421 aggtggcagg attggccaaa tgaaaagtta caacggcatt gttacatcct tcaacagcta
      481 tcgtatctac acttgaagaa aaggctgtgg tatgcgacac ggcattggaa gtaaaactgt
      541 tttcttcgag aaagacacct gaatcaaaag cggagtcgcc ggcatcgccg atggctaatt
      601 tgatatgata agtctggcag ggctgaacca aacaccatgc tgtgtatata taggttagcc
      661 ggtcgtattg aaggtaaaca ccgctgttta accacgagta ggtagctcct gcattacata
      721 cgttattgat ggtaacatag ttggctggat tgtttggcat taccgcaata tttttggcat
      781 taccggtaaa tggcccagta attccggggc cgctgataaa aaagccaaaa gcatcgttat
      841 atgatgagtt gcagaattcg tcaaattctt cggagccata aacatacctg aatttgatag
      901 tgtccgacag gggaataaaa tcaaactcca atacagcttt atcatagatg gtagatgtta
      961 ccagcgactg tagatcaggg tcggagccgc tgcccgttcc tacgcccgag ctgcctgaag
     1021 agttaggccc gatagcatcg gcagcgttgc ccgaagcaag tattatgcct cctgtaaagc
     1081 ctaattgagt ggtggcgcca ccggcagtgg taaagttacc tacctgattt gaattgataa
     1141 gtgctgccga gccgttgaat gtggcattgc tcacggtaac cccgctgcct accagcacct
     1201 gttgaaccag ctgaagcggg gttaatccta tagcggcgcc ctgagcaact gctaattgag
     1261 cctgggcatg ctgctcagtt tttagcgaca acccaattgt tatcaaaaat acaatgataa
     1321 gcgtatattt tttttccatt ggaataggtt tttgtcgtca tagaaaataa ctttttttaa
     1381 gcgttaaata tacgaaataa atcagctaaa tgcaatatca aaggactact ttttatctac
     1441 aaacatataa acgcaataaa agtgcatttt atgagtgaaa acaataaata tatattaatt
     1501 ctgcctgata aaatattttt tcaacttaca atttcttttt cctgttatct tggtgatcag
     1561 ataaaaaaaa tgtagaatgg aaaactacgc caggcgacat gtacaataat atttcccccg
     1621 cgtcttcgta tcaggaaatg aggctatcgt aaaagttttg gtgcaccttt gcaataagaa
     1681 cctttgcctg aaccaattag aaacaccaca ttcgcctgac cacaaagcca ggtgcatatt
     1741 catttttatc agtgtagcgg gctgaaccgg cttaacacaa atttgcgtta aaacgcgtag
     1801 gtcacaccgg ccatcatatt aatcccgtat gcacggtagt tgttccaggt ataatacttg
     1861 atgttggcga tgttgttgac gttgataaag gccgataaaa ttttggagta gcggtattcg
     1921 aagcccaggc ttacatcggc gtatcctttg aaagtctttt ttccggcttc aattcccaaa
     1981 tctgttcttt taaaagtctt ggtataaata tgcgaacgcc cggtaacacc tgccgttatg
     2041 atgaatttgt cgcggatatt gtaccaaaag tccacataag catcaaagat gggtttgtgc
     2101 caggccttaa gctcgttgtc cattttgtaa tgataaaagt tgccgccgac cagcactttg
     2161 aatttttcat ctttgtggta aacaaattca cccctcacat tgagcaggcg ggcgctatcg
     2221 tggattacat tgaacttgtt gcgaaatacc agggtagtat cggtaacgta aagcgggagg
     2281 tttttgatgc gcgaataaga cacggctgca gaaaaattca tggttttgct gatatcgctt
     2341 tttatgcctc cgaaaatatc ataggttgta ctggtgtatg agagcgggat gtaggaattg
     2401 atatatggat tttcgctgat ggtgctgtag aagttgtttt gttgcaggtg gcccttgatg
     2461 cctgcaaaaa ctatcaggat attcttcaca atgttgaact gtacgtcggc gatagggtag
     2521 aagtacaact tggcattgtt ggcggccgat atcgaaatat ctacaccaag attgaaatta
     2581 aaacctttga aagttgtttt tatgtatggt tttaatgaga tgagcccggc atttttttca
     2641 tacagtgaat cctggtcgaa acaatatttt acctgggcct gcaggccaac aaactgtgag
     2701 ctggtgattt taaacaaatg aaggtctttg tttatgtcgg cagtaaaatc aattgcattt
     2761 tcagaggttt tgtaataatc gttcagaaaa tagtatttaa aacctgccga ataatttagt
     2821 ttcagtgaat cgggatggtg tatggtctta aaatacacgt cagcattgat caaattgaaa
     2881 cgttgtttgt aaaggtctct gtcgagcggc agcacggaga tgatatcggg ataatctccg
     2941 ggtttgaatc cataatagtg gtacacattt cttttgtaac caatgcctcc gcctagttca
     3001 tgctgcttaa aaaatttctt tccgtaaaac tcagccagat tttcactgtt acccgggtag
     3061 gcccagtctt tgaattttcc caggtaagaa tggtgtttca ggtgtccgcc aactgaatag
     3121 gaacgagagt aggtattgtt tacaaaaaac tctccgtaag gtgaagtata atttccgaaa
     3181 ccggctttta ccagaaattt gtacagctta gccaggttga tgtcgccaac ccgtgcgggt
     3241 tttatgggat cggtagtata ggtggtgggg tatttgcggg tgagtgtctg gtacgacatc
     3301 ctgggagtaa caaaaatggt gtcgggatgc accggttgca cgttgatttt gttggcgtcg
     3361 gaaattgtgg gctggtaagg ggctattacc gttacttttt catcataggg tttttgtgcc
     3421 tgcagggtgc cgcacacgat ggcaatcagc atgacggcga ctatatattt tggtgttttc
     3481 atgatgtgtt tgttcatttt attgttgtgt ttcatgaaat ttgttttcta ttttatttcg
     3541 ttgagcaggg ggcctggctc gttctgaatt ttcagcaact cttccgcttt cttttgttct
     3601 tcaatttttt cggcctcaag gatggcgttg agtttttctt cagcaatctt gatcaggtcg
     3661 gcgccttcgt aattgtcgat gatgcttttc agggtgtatt ttgcctggtg gcggttgcct
     3721 gtcttcacat agttgtcggc aagcagtata aaggctttgg caacccaata atcataggag
     3781 ggtacctgat ttatcaggtc gaaggccact ttttccgatg cttccgtaag gttttgcagg
     3841 taatatattt ctgcaatact atatttggct tcggcgcagg attcgctttt cgacagtttg
     3901 gtaaccgttt caaattcggt gagtgccagg gtagtgtctt ttgaggcaag cgccgatttg
     3961 gcaatggtaa gatgggcttc caccatcagg tcttcgggta gtttatcggc aagcaaaagt
     4021 ttttgcgaag cactgatagc cgtggaatac aggttgagca ggtaggtgca acgcatttgc
     4081 cctgtctgtg ctgtcagaat attttcttta tactcggcat atttttccag gtcggtatag
     4141 ttagtcaggg cggcttcgta gtttttcctg ctaaagttga tggcggcagc attcagcaag
     4201 gatttctcgg tgaacttggt tctgggtttg tcaatgacaa agttgtagtt ttgcagcgct
     4261 tcgtcggtaa gtttggcacg atactgacat tcggcaaggt aaaaatgtgc gtcaatcagg
     4321 taggcaccat tgggaaattt tgtgatataa tctttaaatc ccgccatggc attgtcgcag
     4381 tcgttacgca tataacgatt ttcaagggca atgtacgtga tagagtcctg ttcggcattg
     4441 gataccgcag caaaaggaag gtctttgacg tacacaaaga aatcttccac gcggtccata
     4501 tcaacataaa cgttgcgaat gctcaccagt gcttctttcg attcgggggt gcccggaaaa
     4561 tcactgacca cttttttgag tacctgcaaa gcctgttcgt ctttttgttc attataataa
     4621 atcatccctt ttttcagcaa ggcctggctg acatagcccg agttgggata ttcccgtgtg
     4681 agcatatcat aatagtcaat ggctttgccg ttgttgttca ggttttgata ggtattggcc
     4741 agttcataga tggcggagac acggtagttt gattttgggt atttgtcgat gagcgacatc
     4801 atggatgaag cttaagtttc gagtttggaa gaagcaccct gtgccagagc tttctgataa
     4861 agggcatagt cggcatcaac caggttcatc tgtaaacttt tatcgtaatt ttcgacggca
     4921 ttggcgaaat ccttgttcat aaaatagcag tcgccggtgc ggttatatgc atcattgatg
     4981 attcggggtg ttgcattttt ggcttccgca gtaaattttt tgaagttcga cagggcagat
     5041 gtataactgg cttttttgaa atacgaataa cccagtccat aatacgaatt gaggtacggt
     5101 tcgttgccga aagctccggt ggtggattgc aagagctgat aattgtaaat ggccgaatcg
     5161 aatttttgct gacggtaata ggcttctgct gtccaataat aggtttgaat ttcgtattcg
     5221 gtgatgatgg ggtatttatt tgattgggct aacatggcga tggcttcatt gtattcattg
     5281 ttattgaaaa gctctattcc gcgcaggtaa gcgatttttt ggtaggcctt gttgagtttg
     5341 ttatcacgtt ttttgatttt ttccaacgat gacagggcgt ctttgtagtt tctggtggta
     5401 aggtatatgt tcgataaata ctcataggct tcatcgaggt tttccgattc ggggtacttt
     5461 tcaatgtatt cattaaagac attcacggcc tcgttgtatg ggttgaaagc cgtttcatag
     5521 gccagtttgg cgtaactgaa catggcattt tcctgcagtt gtttgtctgc atccattttg
     5581 taggccgagg caaacatatt catggcaaat tgtttttggc cggtggcgag gtagcattgt
     5641 gcctggtgat aacaggccag ttgcgaaagg gagtcatttt ccgcggtgct gatttttttg
     5701 aaattttcta tcgctttgcc atattcttct gctttgtagt aggtgtaagc aatcatataa
     5761 tgatctttac gcaggagtgg cgccgtgttt ttttgcaaaa acatttcgag gtagggtagg
     5821 gcttccttgt agcgttcggt atggtaatag gcctcggcgg caatgcgtgc aatttcggga
     5881 gttcttttgc tgttacctgc agaatcgagc aggacaggag ccagggcgat gatttcgtcg
     5941 tacttttgtt gaaaataata gatttgaatc atgtaaaagg gcgctatttt gctgtagttc
     6001 tcgtcgttaa gcaacttgct gaaagctttg agcgcacttt cgtaattttt ttcggtatag
     6061 gcaatatgcg cgtaatagta gttggccggc gccgtatatt tggattcttt gtcgatgatt
     6121 tcaaaaaatg atttcttggc attggcgtaa tcctg
//
LOCUS       DAOSFP010000112         6070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11438_length_6070_cov_4.655362, whole genome shotgun sequence.
ACCESSION   DAOSFP010000112 DAOSFP010000000
VERSION     DAOSFP010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11438_length_6070_cov_4.655362"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PLI16_04515"
     CDS             complement(<1..906)
                     /locus_tag="PLI16_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOH83853.1"
                     /translation="MFSASVYTERRKELKNLFKNGLLLFTGNTELPMNYPQNAYHFRQ
                     DSTFLYFFGLDEPGMAAIIDIDNGKEIIFGDDVDIEDIIWTGPLPLLTEKVKQVGITD
                     SAPFAAYAKYILEAKKQNRCLHFLPPYNPNTKIFLAQLLDIPTEKLKENASMELIQAV
                     IKLRCMKGPEEIGTIEKGMETAYEMFTKAIKMVKPGIYEYEVAGKMEGIARANNGALS
                     FPTILSVRGEILHGHDHSNHMKEGDLLLIDAGFETPDHYATDHTRTVPVSGSFTQKQK
                     EIYQIVLDSQLAAIAAIKPGVKYQEV"
     gene            complement(998..2287)
                     /locus_tag="PLI16_04520"
     CDS             complement(998..2287)
                     /locus_tag="PLI16_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOH83854.1"
                     /translation="MRYKQIPSQLFIRNRQKLARKLKPNSLAIILSSDEYQRNGDQFH
                     PYRQNSDMFYLSGLDQEKCILCLFPDHPREEMRELVFTVQTNDKMVTWYGHKYTLEQA
                     RNVSGVSNVKWLDSFEDMLKDLVLRAENIYLNVYENARFSADVPSNDLRFSRRLKEEY
                     PLHNFERLHPLITECRLCKEPEEIDMIQQACDITEKAFRRMLDRLRPGMMEYEAEAEI
                     THEFVRNGASGHAYAPIVASGINACVLHYIKNDIEIKDGDLVLFDIGAEYGNYAADLS
                     RTIPANGKFTKRQKDCYNAVLRVQKQAKNLIVPGTTIEKINKAVTLLLEEEMINLGLF
                     SRQDVQNQDPDNPLYFKYYMHGNSHFIGLDVHDVGTRQIELKPGMVLSCEPGIYIKEE
                     GIGIRIENDILVSEKGPVDLMKNIPVEADDIERLMMK"
     gene            2433..3098
                     /locus_tag="PLI16_04525"
     CDS             2433..3098
                     /locus_tag="PLI16_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOH83855.1"
                     /translation="MSKFFMYSSITKKILMGIFGLFLALFLLIHLTVNLLMLAGDDGR
                     LFTEASHFMTTNILIKIVEYVLFASFFLHILIGIVLFIQNWFSRPVGYYVVNKSHTEF
                     FSKYMIYTGIIIFIFLVLHFMDFFFIRMEWVQPPAGVDKHDFYRMAVALFQNKAYSFV
                     YIAFFIFLGFHLNHAIMSGFQTLGLNHTTYNKAIRIVSFVYALVIAVGFSTIPVYFMF
                     FYQ"
     gene            3146..5086
                     /locus_tag="PLI16_04530"
     CDS             3146..5086
                     /locus_tag="PLI16_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOH83856.1"
                     /translation="MTKLISKIPDGPLAEKWTTYKSGCKLVNPNNKRKLDIIVVGTGL
                     AGASAAASLGELGYNVKSFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTI
                     KGGDYRAREANVYRLAEVSNAIIDQCVAQGVPFARDYGGLLDNRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYGAMSRQIKKGTVTLYNRRDMLDIVIIDGKARGIIVRNLVSGEIEKH
                     FGHAVVLATGGYGNTYFLSTNAMGSNATPIWRAYKKGAFFSNACFTQIHPTCIPVHGD
                     FQSKLTLMSESLRNDGRIWVPKKKEDVEKLRKKIIMPADIAEEDRDYYLERRYPAFGN
                     LVPRDVASRAAKERCDAGYGVGHTGLAVYLDFKDAISRLGRHVIEERYGNLFQMYEQI
                     IDENPYETPMMIYPAIHYTMGGLWVDYELMTTVPGLYAIGECNFSDHGANRLGASALM
                     QGLADGYFILPYTIGNYLSAEIHTKRIPTDAPEFVEAENEVKMQLQRLKNIKGKKSVD
                     SIHKKFGKLMWENCGMGRNEESLKKGLSMIPEIKKEFWNDLFIPGEMDEYNPELEKAG
                     RVADFIELGELIMRDALQRKESCGGHFREEMQTEDGEAKRDDENFAFVSAWEYKGEDV
                     EPEMHKEELSFEVAKPSQRSYK"
     gene            5196..5504
                     /locus_tag="PLI16_04535"
     CDS             5196..5504
                     /locus_tag="PLI16_04535"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH83857.1"
                     /translation="MEKIDSYKDLKIWQLGIELTVDIYKLSKDFPMEERFGITVQMRR
                     ASSSVPINIAEGWGRKSTKSYSLFLKQARGSLYELETELIIANKIGYLKENPEELLTK
                     "
     gene            5616..>6070
                     /locus_tag="PLI16_04540"
     CDS             5616..>6070
                     /locus_tag="PLI16_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOH83858.1"
                     /translation="MDITLKIWRQKNAKAKGKFETYKVTGVSPDSSFFEMLDTLNQQI
                     IEQGNDPVAFDHDCREGICGTCSLYINGRPHGPKRGITTCQLHMRSFKDGDTIVIEPW
                     RAKPFPIVKDLIVDRSALDLIIQAGGYVSVNTGGIPDANAIPIPKIKSE"
BASE COUNT         1659 a         1253 c         1382 g         1776 t
ORIGIN      
        1 aacctcctgg tatttcactc cgggcttgat agccgcaatg gctgccagtt gtgaatccag
       61 cactatctgg tagatttctt tttgtttttg cgtgaatgag ccgctgacag gcaccgtgcg
      121 ggtgtgatcg gtggcgtagt gatcaggtgt ttcaaagccg gcatctatca gcagcaggtc
      181 cccctccttc atgtgattgc tgtgatcatg tccatgcaat atctcacccc tgaccgacaa
      241 tatggttggg aatgataaag ccccattgtt ggcgcgtgca atgccctcca tttttccggc
      301 tacttcgtat tcgtaaatgc caggttttac catttttatg gctttggtaa acatctcgta
      361 agcggtttcc atgccttttt caatggtgcc tatctcttca gggcctttca tacaccgtag
      421 ttttattacg gcctgaatca gttccatgga ggcattttcc tttagttttt ccgttggtat
      481 gtccagcaac tgggccagaa aaatttttgt gttagggttg tagggtggca gaaaatgcag
      541 acagcggttt tgtttttttg cttccaggat atattttgcg tatgctgcaa aaggtgcgga
      601 atcggttatg ccaacttgtt ttactttttc ggtcagcaga ggcagcggcc ctgtccaaat
      661 tatgtcctct atatctacat catcgccgaa aattatttcc ttgccgttgt caatatcaat
      721 gatagcggcc attccgggct cgtccaggcc aaaaaagtac aaaaacgtac tatcctgcct
      781 gaaatgatag gcgttttgag ggtagttcat cggcaattcg gtattgcctg taaataggag
      841 cagtccgttt ttgaacaggt tctttaattc ttttctcctt tcggtataaa cactggctga
      901 aaacatattt ttaagtttta gaatgataaa taattagttg aattttgatg tgttgatatt
      961 attccacttt cggacttctc cgggctgtca tgtcggctta cttcatcatg agtctttcaa
     1021 tgtcgtcggc ttccacagga atgtttttca tcaggtccac aggccctttt tcgcttacca
     1081 gaatgtcatt ttcgatgcgg atacctatcc cttcttcttt gatatatatg ccgggttcgc
     1141 agctcaggac catcccgggt ttcagttcta tttgccgtgt gcccacatcg tgtacatcca
     1201 gcccgataaa atgagaattg ccatgcatat aatatttaaa atacagtgga ttatccggat
     1261 cctggttttg cacatcctgg cgtgaaaaca gtccgaggtt tatcatttct tcttccagga
     1321 gcaaggtaac agctttgttg attttttcga tggtggtgcc ggggacgatg aggttttttg
     1381 cctgtttttg cacacgcaac accgcattat agcaatcttt ctgacgtttg gtaaatttgc
     1441 cgttggcagg gatggtacgc gaaaggtcag cagcgtaatt gccatattcg gcgccgatat
     1501 caaataatac caggtcgccg tctttgattt ctatatcatt ttttatgtaa tgcaacacgc
     1561 aggcattaat ccccgaggcg acaataggag cataggcgtg gcctgacgca ccgttacgca
     1621 caaactcatg ggttatctcg gcttctgctt cgtattccat cattcccgga cgcagcctgt
     1681 cgagcatcct tctgaaagct ttttcggtga tatcgcaggc ctgctgtatc atgtcaattt
     1741 cttcgggctc tttgcaaaga cggcattcgg taataagagg gtggaggcgc tcaaaattat
     1801 gcaaagggta ttcttctttc aaccgccttg aaaaacgcag gtcgttggac ggtacatcag
     1861 cagaaaaccg ggcattttca taaacattga ggtatatgtt ctcggcccgc agcacgaggt
     1921 ctttcagcat gtcttcaaaa ctgtcaagcc attttacatt gctaactcca gaaacattgc
     1981 gggcctgttc cagggtgtat ttgtggccgt accaggtaac cattttgtcg ttggtctgca
     2041 ccgtgaaaac aagttcgcgc atttcttccc tgggatgatc aggaaaaaga cacaagatgc
     2101 acttttcctg gtcgagaccg ctcagataaa acatgtcgga gttttggcgg taaggatgaa
     2161 actggtcgcc atttctttgg tattcatcac tcgaaagaat aatagccagc gaatttggct
     2221 ttaacttccg ggcaagtttt tggcggttgc ggatgaataa ttgagagggg atttgtttgt
     2281 aacgcattta taaaaatttt tttattaata aaaaattcac tttttatttg gaaaagtcat
     2341 tgtaacagat atctttgtat cgtaaaatac aaagaaaaat aatttttagt aatatagcac
     2401 gttttttcaa tattttttca aaacagaatg ttatgagcaa atttttcatg tattcttcca
     2461 ttacaaaaaa aattttgatg ggtatctttg ggcttttttt agcccttttc ttactgatac
     2521 atctcacggt taaccttttg atgctggccg gcgacgatgg caggctgttt actgaagctt
     2581 cgcatttcat gactaccaat attcttatta aaattgtgga atatgtattg tttgcgtcgt
     2641 tttttctaca tatactgatt ggtatagtat tatttattca aaattggttt tcgagacctg
     2701 taggctatta tgtggttaat aaatcccata ccgaattttt ttctaaatac atgatttata
     2761 ctggaataat tatcttcatt ttcctggtgc tgcattttat ggatttcttt tttatcagga
     2821 tggaatgggt gcaaccgcct gccggagtgg acaagcatga tttttacagg atggcggtag
     2881 ctttgtttca aaacaaggcc tactcttttg tttacatcgc tttttttatt tttctgggct
     2941 ttcatctcaa tcatgctatt atgtcgggat ttcaaacgct tggactgaac cataccacct
     3001 acaataaggc aatcagaatt gtcagttttg tgtatgctct tgtcatagcg gtgggatttt
     3061 ccactatccc ggtatatttt atgttttttt atcaataatt ctattgctgc aatcattttt
     3121 tatacatcaa taatgcaatc cggttatgac aaaactaatt tccaaaatac ccgatggccc
     3181 cttggctgaa aaatggacta cgtataaatc aggctgtaaa ttggtaaatc ccaacaataa
     3241 acgcaagctc gacatcattg tggtaggtac gggacttgca ggggcctccg cggcagcttc
     3301 cctgggcgaa ttaggttata atgtgaagtc gttctgctat caggatagtc cccgccgtgc
     3361 ccatagtatt gctgcccagg gcggtatcaa tgcagctaaa aattatcaga acgacggcga
     3421 cagtgtgtac cgtttgtttt acgataccat aaaaggcggc gactaccgtg cccgcgaggc
     3481 taatgtgtac cgcctggcag aggtgagtaa cgctatcata gaccaatgtg ttgcacaggg
     3541 ggtgcccttt gctcgcgatt atggcggttt gctcgataac cggtcgtttg gcggggcaca
     3601 ggtttcgcgt accttttatg cccgaggtca gacagggcag cagctattgc tgggcgctta
     3661 cggtgccatg agccgccaga taaaaaaagg tacggtgacc ctttacaacc gccgcgatat
     3721 gctggatatt gtcatcatcg atggcaaagc ccgaggcatc attgtcagaa acttagtatc
     3781 cggtgaaata gaaaaacatt tcggccatgc ggtggtgctc gccacgggcg gctatggcaa
     3841 cacttatttt ctttcaacca atgccatggg ctccaatgct acacctatat ggcgcgctta
     3901 caaaaaggga gcgttcttca gcaatgcctg ttttacacag atacacccta cctgtatccc
     3961 ggtgcatggt gattttcagt cgaaacttac cctgatgagc gaaagcctgc gtaacgacgg
     4021 caggatatgg gtgcccaaaa agaaagaaga cgtggaaaag ctgcgcaaaa aaatcattat
     4081 gcctgccgat attgctgaag aagaccgtga ctattacctc gaacgaagat accctgcctt
     4141 tggcaacctg gtgccgcgcg atgtggcttc gcgtgctgcc aaggagcgct gcgatgccgg
     4201 ttatggcgtg ggacataccg gactggcggt ctacctggat tttaaagatg ctatctcgcg
     4261 attgggaagg catgttatag aggaacgcta cggaaacctt ttccagatgt atgaacaaat
     4321 catcgacgaa aacccttatg aaaccccgat gatgatttat ccggccattc attatacaat
     4381 gggcgggttg tgggttgact acgagttgat gaccacggta ccgggacttt atgccatcgg
     4441 tgagtgtaat ttttcagatc acggagccaa ccgtctgggc gcctccgcct taatgcaggg
     4501 gctggcggac ggatatttta tcctgcccta tactatagga aattatttgt cagccgaaat
     4561 tcataccaaa cgcattccga ccgatgctcc cgaatttgtc gaggctgaaa atgaagtaaa
     4621 aatgcaactt cagcggctga aaaatatcaa aggcaaaaaa tctgtggatt ccatacataa
     4681 aaaattcggc aaactgatgt gggaaaactg cggcatgggg aggaacgaag aaagcctgaa
     4741 aaagggattg tcgatgatac ccgaaataaa aaaagagttc tggaacgacc tttttatacc
     4801 cggcgagatg gatgaatata atcctgagtt ggaaaaagcc ggaagggtgg ctgattttat
     4861 cgaacttggc gagctgatta tgagagatgc cttacaacgg aaagaatcat gcggcggtca
     4921 ctttcgcgaa gaaatgcaaa ccgaagatgg tgaagcaaaa cgtgacgatg aaaattttgc
     4981 atttgtttct gcatgggaat acaaaggcga ggatgtagaa cccgaaatgc ataaggaaga
     5041 actgagtttt gaggtggcaa agccttcgca gaggagttat aagtagctat tagcgattgg
     5101 ccattagcca ttagccatta gccattagtc attagccaat agcaaaaagt aagaagccga
     5161 tagctaatta ataagggtag taggaaaaaa ataaaatgga aaagattgat tcatacaaag
     5221 atttaaaaat atggcagctt ggcattgagt tgactgtgga tatttataaa ttaagtaagg
     5281 attttccgat ggaagaacgt tttggaatta cagttcagat gagaagagca tccagttcag
     5341 ttccgataaa tattgctgag ggatggggca gaaaatccac aaaatcgtat tctctatttt
     5401 tgaaacaagc aagaggctca ttatacgagc ttgaaacaga actaataatt gcaaataaaa
     5461 ttggttacct aaaagaaaac cctgaagagc ttttaacaaa atagtttcac tctcaaaaat
     5521 gattaataca ttaattaaaa ctttagaaaa taaaaccaat gataaatagt ggcttatggc
     5581 ctatggccat tggctgaaaa ataaaataat taatcatgga cataacctta aaaatctggc
     5641 gtcagaaaaa tgctaaagca aaaggaaaat ttgaaaccta taaagttaca ggagtgtctc
     5701 ccgacagctc tttctttgaa atgctcgata ccctgaacca gcaaatcatt gaacagggga
     5761 acgatccggt ggctttcgac cacgattgcc gcgaaggaat ttgtggcacc tgtagtctat
     5821 acatcaatgg caggccacac ggtcccaaac gtggtattac cacctgccag cttcatatgc
     5881 ggtcgttcaa agacggtgat accattgtga ttgaaccctg gcgggccaag cctttcccaa
     5941 ttgttaaaga ccttatcgtg gaccgttcgg cgcttgatct gattatacag gccgggggat
     6001 atgtctctgt gaataccggc ggtatccccg atgccaacgc tattcccata ccaaaaataa
     6061 aatcagaaat
//
LOCUS       DAOSFP010000113         6045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11512_length_6045_cov_3.914858, whole genome shotgun sequence.
ACCESSION   DAOSFP010000113 DAOSFP010000000
VERSION     DAOSFP010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11512_length_6045_cov_3.914858"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="PLI16_04545"
     CDS             complement(<1..1129)
                     /locus_tag="PLI16_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83859.1"
                     /translation="MKRILFVLALLSYFSGSFAQFQVGHRTITFQDPARSNRNIETEV
                     YYPGTTAGDNAALANGQFPIVVYGHGFLMGVSAYENVWTVLVPMGYIVALPKTESGTP
                     DHLEFGRDLAFLINQLKSEGTNSSSPFYQKVSATSAIMGHSMGGGAAFLACENNSVPN
                     VMVTFAAANTNPSSIHAATNVSIPAMVIAGEMDCVAPPDEHQTPMFDSLSSPCKTFIS
                     IKNGGHCNFADYNFICTTGEESCHPGGVPLSRAEQQLTAMNFVMPYLDYFLKGNNASW
                     NNFLDSLNNSTKVTRIHSCTINPSAINTNECSLPAMTLVPNPTSGQLNISFAEDKEVH
                     IEITGITGNKLFNTCHKGRQVCIDISHFCQSIYWITLDDENG"
     gene            complement(1197..2726)
                     /locus_tag="PLI16_04550"
     CDS             complement(1197..2726)
                     /locus_tag="PLI16_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOH83860.1"
                     /translation="MFKNHPKGLLSAALANMGERFGFYTMMAILVLFLQAKFGLSGPN
                     AGIIYSIFYFSIYILAFVGGLIADKTKNFKGTILIGLIMMAVGYFLIAIPTSTPVPEN
                     SFNLLLGMTCGGLFVIAFGNGLFKGNLQALVGQMYDNEKYGKLRDSGFSIFYMFINVG
                     AIFAPLAAVGVRNWWVTSHGFEYNSAMPELCHSQLNNTISAEGLTKLQEFAAQFNYAG
                     TDITTFANKYLNVFATGFHYAFAVAICAMLISLIIYIANKKKFPNPKTKEEVANANIK
                     EMDAKEVKQRLYALFAVFAVVIFFWFSFHQNGLTLTYFAKDYTDLSKISINLGFTEIK
                     GAELFQSINPFFVVFLTPVILAVFGALRARGKEPSTPKKIAIGMGIAASAYLLMALGS
                     MGIPTKSELTEMGGLPDAQRITPFLLLGTYFILTVAELFISPLGISFVSKVAPPKYQG
                     MMQGLWLCATAVGNSLLFIGAVLYDIIPISGVWTVFIVACCISMGTMFFMLRWLERVA
                     K"
     gene            complement(3054..4358)
                     /locus_tag="PLI16_04555"
     CDS             complement(3054..4358)
                     /locus_tag="PLI16_04555"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH83861.1"
                     /translation="MGLNKYIPKGFTAIKHIFSSLHSRNYRLYFIGQGVSLIGTWMQN
                     IALSWLVYRLSGSVFLLGLVGFTSQIPSFILSPFSGVISDRYNRLKLMKLAQIFFMLH
                     ALAMAVLVLTGAIQVWHIVTLSIVFGIINAFDAPARQSLVIDLIDNPKDLGNAIALNS
                     AIFNAGRLIGPAIAGITIAIVGEGICFLLNALSFVAVIAALMQIRIPARQQSIIPGKF
                     KKSFSEGFHYTFHSMPIRTLITMLAVLSLIGLPVVVLLPAYAKEILHGNADTLGYLMS
                     ALGAGALCGALYMASRKTVLGLGRIISVSIGIYGLALTLASFSHIAYLSMMIFFFAGL
                     TMVLSLSSINTMLQTIADEDKRGRVMSFYAMALMGTTPIGNLLSGTLASGIGIPYTLL
                     ISGTITILSGVWLWVNLKALRKYVRPIYVNKGILPGLPHDIN"
     gene            complement(4657..4947)
                     /locus_tag="PLI16_04560"
     CDS             complement(4657..4947)
                     /locus_tag="PLI16_04560"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOH83862.1"
                     /translation="MTSSEREKKTVYLMIGMYCKKQRHHAEMCEECRQLTLYAYEKID
                     QCRYNPKKPKCSKCTTHCYQKDKREKIRQVMRYSGPRMILYHPLVALRHMLR"
     gene            complement(4962..>6045)
                     /locus_tag="PLI16_04565"
     CDS             complement(4962..>6045)
                     /locus_tag="PLI16_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83863.1"
                     /translation="VISINPQNLLAYFYRAEIRQQQNDLIGAIDDYSKVISLYPAFYH
                     AYYMRSMLYRQINDYDGYRRDKMMAEHLMNSTDTTLYNETQLNSFKSLFELRTEVENA
                     DTSSGKIQYKSYEISIKPLYYLMAADPAQQAALPLTPKDYNAQLAELNSLSEILLHLE
                     FIHNDQQAGKEKIIKERDRLQEITLKENNNEALLIWSSILSASLFNYSEALTLLTGIS
                     DTSGFSYLAWFIRGNIHYDLGQNLEQNQKNMDLISGNDTKDRNDYYYKALEDYTAAIE
                     KNNKFTFAYFNRAYVKTMLNDYSGAILDYSVSIFYNTSFAEAYFSRGLLYLFTGNIEK
                     GCEDISKAGELGLKESYNLLFKHCKK"
BASE COUNT         1755 a         1266 c         1338 g         1686 t
ORIGIN      
        1 gaccattttc atcatccaag gttatccaat atatactctg gcagaaatgg ctgatgtcga
       61 tacatacctg ccggccttta tgacaggtat taaacagttt gttcccggtg atacccgtaa
      121 tttcaatatg tacctcttta tcttctgcaa agctaatatt cagctggcct gaagtggggt
      181 tggggactaa ggtcatggca ggcagcgagc attcattggt gtttatcgct gatggattga
      241 tggtgcaact gtggatgcgg gtaactttcg tggagttgtt taatgaatca aggaagttgt
      301 tccatgaagc gttgtttcct tttaaaaagt aatccagata aggcataaca aaattcatgg
      361 ccgtaagctg ctgttcggcc cgcgacaagg gtacaccgcc gggatgacag ctttcttcac
      421 ctgtagtgca tataaaattg tagtcggcaa agttacagtg tccgccgttt ttgatgctga
      481 taaaggtttt acatggtgaa gaaagcgaat cgaacatggg tgtctggtgt tcatcgggtg
      541 gcgccacaca atccatttcg cctgctatta ccatggccgg gatgctaaca ttggtggccg
      601 catgaattga cgatgggttg gtattggcag cggcaaaggt aaccatcacg ttaggtacgg
      661 aattgttttc gcaggccagg aatgcagcac cgccacccat cgaatgcccc ataatggcgg
      721 aggtggcgct gactttctga taaaaaggcg acgagctgtt tgtcccttct gattttaatt
      781 gattgataag gaaagcaagg tcacgaccaa attccaaatg atctggtgtt ccgctttctg
      841 ttttaggcag ggccacgata tatcccatgg gaacgaggac cgtccataca ttttcatatg
      901 ccgatacacc catcaggaag ccatggccat aaacaactat ggggaattgt ccgttggcaa
      961 gggcagcatt gtcgcccgcc gtggttcccg gataataaac ttctgtttcg atattgcggt
     1021 tgctacgcgc cggatcctgg aaagtgatgg tgcggtggcc aacctgaaat tgtgcaaaag
     1081 aacctgaaaa ataagataac agggcaagaa caaataaaat tcttttcatt gttttgatat
     1141 tttcgggttt aggaattaat aaaaaaaaaa gaggccgtta agcctctttt ggaattttat
     1201 ttggcaactc tttccagcca tcgtagcatg aagaacattg tccccatcga aatacagcag
     1261 gccacgatga aaacggtcca tacgccggaa ataggaataa tgtcgtacaa tacagctccg
     1321 ataaacagca gcgagtttcc gacagcagtg gcacaaagcc ataatccctg catcataccc
     1381 tgatatttgg gaggagccac tttcgaaacg aaggatattc ccaacggcga aatgaagagt
     1441 tcggccacag tcaggataaa ataagtgcct aacagcagga agggtgtgat acgctgagca
     1501 tcgggcaatc ctcccatttc tgtaagttcg ctttttgtag gaattcccat tgaacccagt
     1561 gccattaaca gataggcact tgcagcgata cccattccga tggcaatctt tttaggagtt
     1621 gaaggttctt ttcctctggc ccgtaaagcg ccgaaaacag ctaaaatcac cggtgtaagg
     1681 aatacaacaa aaaatggatt aattgactgg aacaattctg caccttttat ttcggtaaac
     1741 ccaaggttaa tactgatttt gctcaggtct gtgtagtctt ttgcaaaata agtaagtgtt
     1801 aacccattct gatggaatga gaaccagaag aaaataacca cggcaaaaac tgcaaacaat
     1861 gcataaaggc gctgcttgac ttctttagca tccatttctt taatattggc attggcaact
     1921 tcttccttgg ttttaggatt cgggaatttc tttttgttgg caatgtagat aatcagtgaa
     1981 attaacatag cacagattgc tactgcaaaa gcataatgaa aacctgtagc aaacacgtta
     2041 aggtatttat tggcgaaagt tgttatgtcg gttccggcat aattaaattg tgcggcgaat
     2101 tcctgaagtt tggttaggcc ttctgcgctg attgtgttat tcagttgtga atggcacaat
     2161 tccggcattg cagagttgta ttcaaatcca tgagacgtta cccaccagtt ccttacgcct
     2221 acagctgcaa gtggcgcaaa gatggcaccc acattgataa acatataaaa tatggaaaat
     2281 ccagaatcac gtaatttccc atatttttcg ttgtcgtaca tctgaccgac aagagcctgc
     2341 aggttgcctt tgaacaaacc gttaccgaag gcaataacaa acaatccgcc gcaggtcatc
     2401 ccaagtaaaa gattaaaaga attttcagga accggagttg atgtagggat agcaatcaga
     2461 aaatacccga cagccatcat aattaagcct ataaggatag tgcctttaaa gttcttggtt
     2521 ttatcagcaa tgagtccgcc aacaaaggca agaatgtaaa tcgaaaagta aaaaatggaa
     2581 taaatgattc cggcattggg gcctgaaaga ccgaattttg cctgaagaaa cagcacgagg
     2641 atggccatca tggtgtaaaa cccgaaacgc tctcccatat tagccaacgc tgccgacaat
     2701 aaacccttag gatgattttt aaacatagtt ttttagtttt aaattataag gtgcaaacat
     2761 agtcaaattt tttaattaga caagcataaa ttttatgttt ccttgaaata tgtggtttat
     2821 aaatgtttat tgattacgac aatactgtaa tcccttaacc tgaaaaattc ataagaattt
     2881 ttcacccttc gagcagacaa aagaaaatag ggtaaccctg caatgattgg cttctttata
     2941 tattttcttt gtcgggattt acctaacctg ctgcccacat cgacctccca aataaacaaa
     3001 tttcacttct ttcatttgtg aaaaggtctg gttaaaaatg caatttttcc aaattaatta
     3061 atatcatgtg gtaatcccgg gagtatgcct ttattaacgt atatagggcg cacatacttc
     3121 ctgagcgctt tgaggtttac ccaaagccat acgcctgata aaatcgttat ggttcctgaa
     3181 atcagcaatg tgtaaggtat accaatccca ctggccagcg ttcctgaaag gagattgcca
     3241 atgggtgtcg ttcccatcag cgccatagca taaaaactca tcacccgccc ccgtttatct
     3301 tcatcggcaa tggtctgaag catcgtgttt attgaagata atgaaagaac catagtgagc
     3361 ccggcgaaga aaaaaatcat cattgaaaga taggctatgt gcgaaaatga tgctaacgtt
     3421 aaagccaggc cataaattcc gatgcttaca gaaattattc ttcccagccc cagcactgtt
     3481 ttgcgtgatg ccatatacag cgcaccgcac agtgcgccgg cgcccagggc ggacataagg
     3541 tatccaagag tatcagcatt tccatgaagg atttctttgg catatgccgg taaaagaact
     3601 accaccggaa ggccgatcag gcttaaaaca gccaacatgg taatcaaagt gcgtatcggc
     3661 atggaatgga aggtataatg gaacccctcc gagaaactct ttttaaattt tccgggaatg
     3721 atggactgtt gtctagccgg aatcctaatc tgcatcagtg cggcaataac cgcgacaaaa
     3781 ctcagggcat tcaacagaaa acagatgccc tcaccaacta tggcaatggt aatacctgca
     3841 atagccgggc ctattaaacg cccggcgttg aaaattgctg aattcaatgc gatggcgttc
     3901 cccaggtctt tggggttatc aatcagatcg atgaccagtg attgccgggc cggtgcgtca
     3961 aaagcgttga taataccaaa aacaatactt aaggttacga tatgccatac ctgtatggcg
     4021 ccggtcagta ccaaaacagc catagccagt gcatgaagca tgaaaaaaat ctgtgcaagt
     4081 ttcattaatt taagccggtt atacctgtca ctgatcaccc ctgaaaaagg tgaaagaata
     4141 aaactgggta tctgactggt aaatcccacc agacctaaaa gaaataccga tcccgaaagc
     4201 ctgtaaacca gccagcttaa tgcaatattc tgcatccatg ttccaatcag ggaaacacct
     4261 tggcctatga aataaagccg gtagtttcgc gaatgcagcg atgagaaaat gtgctttatc
     4321 gccgtaaaac ctttcggaat atatttgttg agccccatgg ccggtaataa ttaatgtaag
     4381 tgtgttatga accgcataca tttttgtatg acaaagttcc gaataaatct ttagttgata
     4441 ggaattatat cagacaatta tctaatgcta gtgcctataa gaaaactcgt gtttgtacaa
     4501 caaaacgtca agtttgaggc ctgcctgact gaagtcattc aggcaggcct gcgaagcgac
     4561 aaaaagcgga gtatactggt gcaaatgagc atttttgtcg caagcgtaac cctgcccgat
     4621 gcaggcgggg aaaaaattga cgttttccta caaacactat ctaagcatgt gccggagcgc
     4681 taccaacggg tgatacaaga tcatcctggg ccctgaataa cgcattacct gccttatttt
     4741 ctcccgttta tctttttggt agcaatgagt tgtgcacttg ctgcattttg gctttttagg
     4801 gttgtatcgg cattgatcaa ttttttcata agcataaagt gtgagttgcc ggcattcttc
     4861 gcacatttcc gcatgatggc gttgtttttt acaatacatc ccaatcatga gatacactgt
     4921 ttttttttcc cgttcggaac ttgtcatcat aaacacacga tttatttttt acaatgtttg
     4981 aacaagaggt tataggattc ttttagcccc aattctccgg ctttactgat atcctcgcat
     5041 cctttttcaa tattgccggt aaatagatat aacaacccac ggctaaagta ggcttcggca
     5101 aatgaggtgt tgtaaaaaat acttacggaa taatcgagta tggccccact gtaatcgttc
     5161 agcatggtct tgacataggc acggttgaaa tacgcaaatg taaatttgtt attcttttct
     5221 atggctgccg tataatcttc cagggcctta taataatagt cgtttctgtc tttagtgtca
     5281 ttgccggaaa tcagatccat atttttctgg ttctgttcca ggttttgccc caggtcataa
     5341 tgaatattgc ccctgataaa ccatgccagg tatgaaaatc cggaagtgtc gctgatgccg
     5401 gtcagcaggg tcagggcttc gctgtaatta aacaacgagg cagataaaat actgctccag
     5461 atcagaagcg cctcgttgtt gttttcctta agggtaattt cctgcagccg gtcacgctct
     5521 ttaatgattt tttctttgcc tgcttgttgg tcgttatgaa tgaattcaag gtgcagcagg
     5581 atttcactta acgagttaag ttcggcaagt tgcgcattgt agtccttggg cgtgagcggt
     5641 aaggcggcct gttgcgccgg atcggcagcc atcaggtaat aaagcggctt aatggaaatc
     5701 tcataagatt tgtattgaat tttaccgctg gaggtatcag cgttttcaac ctcggtgcgt
     5761 agctcaaaca aacttttgaa cgagttgagc tgggtttcgt tgtacaaggt ggtatccgtg
     5821 ctgttcatca ggtgctcggc catcatcttg tcgcggcggt atccatcata atcattaatc
     5881 tgacggtaaa gcatagagcg catataatac gcatgataga atgcggggta caggctgatg
     5941 acttttgagt aatcatcaat tgcgcctatc aggtcgttct gttgctgtct tatttcggca
     6001 cggtaaaaat aggcaagcag gttttggggg ttgatgctga taaca
//
LOCUS       DAOSFP010000114         6018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11601_length_6018_cov_3.757672, whole genome shotgun sequence.
ACCESSION   DAOSFP010000114 DAOSFP010000000
VERSION     DAOSFP010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11601_length_6018_cov_3.757672"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PLI16_04570"
     CDS             <1..817
                     /locus_tag="PLI16_04570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH83864.1"
                     /translation="FGNYFLSRPEPQACWNKSLKQAEWEKLAHAKFVKTAGKESLGEK
                     GQWHFLKKMDEQWWVGYVYKSMKLRFRLGLTAFKHVGLFPEQADNWNYIFDSVSEIKE
                     IHPRVLNLFAYTGGASLAARAAGADVAHVDSVRQVVNWSRENMEASGLDGIRWLVDDA
                     MKFVKREVRRGNKYHGIILDPPAYGRGPDGEKWVLEEQINELIKNCSELLSEEKNFLI
                     LNLYSMGFSALVAENLICDYVKNIKTKEFGELFFADHSGKRLPLGTFFRMRSL"
     gene            complement(831..2021)
                     /locus_tag="PLI16_04575"
     CDS             complement(831..2021)
                     /locus_tag="PLI16_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83865.1"
                     /translation="MKNYLIVLIAVLSIHTLSKAQPFLPEETVVVFQTSTPAPGMTNK
                     CLLFKAGNDFGWIDPNGVLHKSKILKITAGFLLVDSDTVFPADIKSLALSGPAAFFLS
                     KNQERFVPVKSDSTGLFRVMSFQEFEKHSKTEFLKEAKEHNDPFYKHIPAEKYAADMD
                     KKMKRRKNMFAALDTCPLRYGLSTNLVRDLTNEINLNFELPVKRNFCIEFGAGILYAK
                     PDASRYDFAYFLTTLTNIRGRHITMFDQSYLYRKGFSLEAIPKFFLSKKKHLYLGPQL
                     GFRYYYYTDKLIFLDADGSDYYHISSYAIQSEKSAAVNLNMILGVQTPQIKRFLFNAF
                     FSIGMMYRGGVVSRSEHIEYHHEGSVKEYYDPPIKLKDGGIFLSVQLGFKIGWRFGKA
                     KLYG"
     gene            2361..3971
                     /locus_tag="PLI16_04580"
     CDS             2361..3971
                     /locus_tag="PLI16_04580"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOH83866.1"
                     /translation="MAAFIYEKPFQTEKDTTRYRLLTKDYVELVDAGGRKILKVDPKG
                     LELLAKEAIFDLSFFLRTSHLEKLAKILDDPEATDNDRFVAYTMLKNTVISAGGELPW
                     CQDTGTAIVVGEKGEQVWTCGHDAESLSKGIHATYQEKNLRYSQVVPFTMFEEKNSGT
                     NMPAQIDIYATEGISYKFLFITKGGGSANKTFLYQQSKSLLNEESLEKFIREKIRDLG
                     TSACPPYHLAFVIGGTSAEATLTAVKKASAGYYDLLPTMGNASGRAFRDIEWEKKIEN
                     ICRDYGVGAQFGGKYFAHDVRVIRMPRHAASCPLGMGVSCSADRNLKAYINENGIFIE
                     ELEKNPERFMPAKAPELEKAVEINLDRPIKEVLAELTKYPVKTRLSLTGTLIVARDIA
                     HAQIKKLLDEGKPMPEYFKNHPIYYAGPAKIPQGMPSGSFGPTTAGRMDSYVDLFQSL
                     GGSLIMVAKGNRNRAVIDACKKYGGFFLGSIGGPAALLAAENIKSVEVVAFEDLGMEA
                     IRKIRVENMPAFILTDDKGNDFFDEYNK"
     gene            4779..5174
                     /locus_tag="PLI16_04585"
     CDS             4779..5174
                     /locus_tag="PLI16_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83867.1"
                     /translation="MKNILLIIICALFTINSQASTITTHTTISAEELIISSDPPLYGF
                     VVDTTITGFGTLTYPNGEIFVGKFRKGLKHGNGYIKFPDGREYHGDFKNDKIEGYGTM
                     VYPNGDKYVGNFKAGKYEGNEALTTVTGK"
     gene            complement(5236..>6018)
                     /locus_tag="PLI16_04590"
     CDS             complement(5236..>6018)
                     /locus_tag="PLI16_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH83868.1"
                     /translation="HSVSGKLAKNASLISVRPFRSPHHTISDVALVGGGSFPQPGEIS
                     LAHNGVLFLDELPEFKRTVLEVLRQPVEDRVVTISRAKFSVEYPASFMLVAAMNPCPC
                     GYYNHPEKECVCGPGIVQKYLNKVSGPLLDRIDIHVEVTPVPFKELSKAQASEKSAVI
                     RERVVMARRIQETRFGEIQGVYCNAQMKTKHLQTYCAIGETGLTLLKNAMEKLSLSAR
                     AYDRILKVARTIADLDNSAEIQNHHLAEAIQYRSLDRENWAG"
BASE COUNT         1818 a         1261 c         1263 g         1676 t
ORIGIN      
        1 tttcggaaac tattttttat cgcgcccgga accccaggcc tgttggaaca aatctttaaa
       61 acaagccgaa tgggaaaaac tggcccatgc aaaatttgtt aaaaccgcag gcaaagaaag
      121 tcttggagaa aaaggacaat ggcatttcct aaaaaaaatg gacgaacaat ggtgggtggg
      181 ttatgtgtac aaatcaatga aattaaggtt ccgtcttgga ttgaccgcat tcaagcatgt
      241 gggcttgttt cccgaacagg ccgataactg gaattatatt tttgattcgg tttccgaaat
      301 aaaagaaatt catcccaggg tcctgaattt attcgcttat acaggcggcg cttcgcttgc
      361 ggcacgggct gcaggggccg atgtggctca tgtggattcg gtgaggcagg ttgtcaactg
      421 gtcgcgcgaa aacatggagg caagcgggct tgacggcata cgatggctgg tggatgatgc
      481 catgaagttt gtaaaacgtg aggtacggcg cggaaacaaa taccacggga tcatccttga
      541 cccaccggct tacggccgcg gacctgacgg cgaaaaatgg gtgctcgagg aacagatcaa
      601 cgaattgata aaaaactgtt ccgaattatt atccgaagaa aaaaacttcc tgatcctgaa
      661 cctttactcc atggggtttt cagcccttgt tgcagaaaac ctcatctgcg attatgtaaa
      721 gaatataaag acaaaagaat tcggggaatt gtttttcgcc gaccatagtg gaaaaagact
      781 tccgctgggg acattcttca gaatgaggtc tttgtgaaaa aatgtttttt tcatccataa
      841 agttttgcct tcccaaaacg ccagccaatc ttaaatccca gttgcaccga taaaaatatc
      901 cccccgtctt tgagcttaat aggcggatca taatactctt taacagaacc ttcatgatga
      961 tattctatat gctcgcttct gctgacaaca ccacctctgt acatcatgcc tattgaaaaa
     1021 aaagcattga ataaaaacct ttttatttgt ggggtttgaa cacccaggat catattgaga
     1081 ttaaccgcag cgcttttttc actttgaatc gcataagaag atatgtggta ataatctgaa
     1141 ccatcggcat ccagaaagat caatttgtca gtatagtaat agtagcgaaa accaagctgc
     1201 gggcctaaat acaaatgttt ctttttcgaa agaaaaaatt tgggaatagc ttcaagggaa
     1261 aatcctttgc gataaagata agactgatca aacatggtaa tgtgtcgtcc cctgatattt
     1321 gtgagtgttg ttaaaaaata tgcaaagtca taacggctgg cgtctggctt tgcatagaga
     1381 atacctgcac cgaattcaat acagaaatta cgcttaaccg gtaattcaaa attcaggttg
     1441 atttcgttgg ttaggtctct gacaagattt gtacttaagc cgtaacgtaa cggacaggta
     1501 tcgagtgccg caaacatatt ttttctccgt ttcatttttt tatccatatc ggcagcatat
     1561 ttttctgcag gaatatgttt ataaaacgga tcgttatgtt ccttcgcttc tttgagaaat
     1621 tccgttttgg aatgtttttc gaattcctga aagctcataa cgcgaaagag tcccgtgctg
     1681 tcggatttaa caggaacgaa cctctcctgg tttttagaaa gaaaaaaagc agccggccct
     1741 gataatgcca ggctctttat atcggcggga aaaacagtat ctgaatctac cagtaaaaaa
     1801 ccggcagtga tttttaaaat tttggatttg tgtaaaacac cattaggatc aatccaccca
     1861 aaatcattcc ctgctttaaa tagtaagcac ttgttcgtca tgcccggagc tggagttgat
     1921 gtctgaaaaa ccacaaccgt ttcttccggc aaaaatggct gggcttttga caaagtatga
     1981 atcgacaaaa ctgcaattaa aacaattaaa taatttttca ttgaaagcca tttttttaat
     2041 acctaatctt catttctaaa tactcttacc ccttttttca tttaaaaatt gaacaaaatg
     2101 taatattagt aatttttttt aattgaatac gacttttttc aatctttcaa tccgaggagt
     2161 ataattatct aacacgtacc agatattcat gagccgccgt atagacttac tttcctgaca
     2221 tttatcaaaa agaaaaattc atgaaaaata ttttggccgg aatatttttt tattgttaaa
     2281 aattttttta tgtttgagct gtcgttaatt ttttattaat attttaatat tcaataaatt
     2341 gttttataaa tcactgaatt atggccgcat ttatttatga aaaacctttt cagactgaaa
     2401 aagacaccac ccgatacagg ctattgacaa aagattatgt tgagcttgtt gatgccggcg
     2461 gaagaaaaat actcaaagtt gatccaaaag gcctagagtt acttgccaaa gaagcaatct
     2521 ttgatctttc attttttctt cgcacttccc atctggaaaa gctagccaaa atccttgatg
     2581 atcccgaagc caccgacaac gaccgttttg tggcttatac catgctgaaa aataccgtca
     2641 tttcggccgg aggagaactt ccctggtgcc aggatacagg aacggcaatt gttgtcggag
     2701 aaaaaggcga gcaggtgtgg acctgcggcc acgatgccga atcgctttcg aaaggcatcc
     2761 atgctacata ccaggaaaaa aatctgaggt attcacaggt ggtgcccttc actatgtttg
     2821 aagaaaaaaa cagcggtacc aatatgcccg cgcagataga tatttatgcc actgaaggca
     2881 tttcttacaa gtttcttttt atcacaaaag gcggtggctc ggcaaacaaa accttccttt
     2941 accaacaaag caaatcgctg ctcaatgaag aatcgctgga aaaatttata cgtgaaaaaa
     3001 tacgcgacct gggcacctct gcctgtcctc cttatcacct ggcctttgtc attggcggta
     3061 cctctgcaga agccacgctc acagccgtca aaaaggcttc cgccggctat tatgatctcc
     3121 tccctaccat gggaaatgcc agcggaaggg ctttccgcga tatcgaatgg gaaaaaaaga
     3181 ttgaaaacat ttgtcgtgat tatggtgttg gtgcgcagtt tggcggtaaa tactttgccc
     3241 acgatgtgcg tgtcatccgg atgccgcgcc atgcggcttc ttgtccttta ggaatgggcg
     3301 ttagctgtag cgccgacaga aatttaaaag cctatataaa cgaaaacggc atttttattg
     3361 aagaactcga aaagaatcct gaacgtttca tgcctgctaa agccccggaa ctggaaaaag
     3421 ctgttgaaat aaacctggac aggcccataa aagaagtgct tgccgagctt acaaaatatc
     3481 ctgtaaaaac gcgattgagc ctgacaggaa cactaattgt tgcgcgggac attgcccatg
     3541 cacagataaa aaaacttctt gacgagggaa aacccatgcc ggagtatttt aaaaatcacc
     3601 ctatttatta tgccgggccg gcaaaaattc cgcaaggcat gccatcagga agcttcgggc
     3661 caaccacggc ggggcggatg gactcttatg tggacctctt tcagagtctg ggcggctcgc
     3721 tgatcatggt tgccaaagga aacaggaaca gggcggtgat tgacgcctgc aaaaaatacg
     3781 gaggtttttt cctcggttca ataggtggcc cggctgccct gcttgctgct gaaaatataa
     3841 aatcggtgga agtggtagcc tttgaagacc ttggcatgga agcaatcaga aagatcaggg
     3901 tggaaaatat gcctgccttt atacttaccg atgacaaagg caatgacttt tttgacgagt
     3961 acaacaaata aaaaaaaatc ctgactgaat aaaatcaggc cttatatctg tttcccaatt
     4021 tatcggccca gcgttcagga aaatacctga aataacggtt gcttaccctg gttgaaatcc
     4081 gggagagtat agaaagcagc agggaaggaa ccccaattaa aaataaatac agaggcccca
     4141 gcaaataacc ctgtatagtg tgtccatact catgaagaat cgtattcttt tcaaatattc
     4201 tatcggaaac aatgataaac ttacaagtga aataccgcct atcaaaaaca aattttccga
     4261 cctgaccagg catacatagt taaaccggct tttttcgagc agacggccgc tgacttttac
     4321 ataaaggaat atagccaggc ccactaatga ctgaggcaaa caccatgtcc aaaatatcag
     4381 gttgaaaaga aataatttaa ttcttgaagg ctgtcgcatt attttttttt ggatgttttc
     4441 tatcaaaaat tgcgcccgtt tttgacctgg tctaattacc aataaacgca gtgatattga
     4501 tgaatgggtg tggacaacgc ggaatgcagt ttcggttagc ttttagaact gctgcaccat
     4561 catcattcgt caatcgatgt tcgatattga aatgaattaa atgatgtatg aattttgaca
     4621 atagatgttc gattggcaga ttaccacatt ttcttttttc ggcccgaagg gtgaattttt
     4681 attgtaaatc tttcagatta aaattttttt tgaaaatgtt ttttgtttca aaaaaatgtg
     4741 tacctttgcc ggccttttca aaattaaaac ataaatcgat gaaaaacata ctgttaatta
     4801 ttatttgcgc cttattcacc ataaatagcc aggccagtac aataaccacc catacgacta
     4861 ttagcgctga agaattaatt atcagcagcg accctccttt atatggtttt gtggttgaca
     4921 ccactatcac cggttttgga acacttactt atccgaacgg cgaaatattt gtcggcaaat
     4981 tcaggaaagg acttaaacac ggcaatggat atattaagtt tccggatggc cgcgaatacc
     5041 atggcgactt taaaaatgac aaaatagaag gttatggcac catggtttat cccaacgggg
     5101 ataaatatgt cggtaacttt aaagctggca aatacgaagg taacgaagca ctgaccacgg
     5161 ttacaggtaa ataagaacaa ggattattca aaaacaactc cattttagaa taaacagcgg
     5221 tctttcctgc cgaatttatc ccgcccaatt ctccctgtca agactacggt actgtatggc
     5281 ttcggccagg tgatggtttt gtatttcagc cgaattatcc aaatcggcaa tggtgcgggc
     5341 aaccttcagg atacgatcat aggcccttgc tgataaactc agtttttcca tagcattttt
     5401 caacagggtt aatccggttt cgccgatagc gcaatatgtt tgcaggtgtt ttgttttcat
     5461 ttgtgcatta caatacacac cctgaatttc gccaaagcgc gtttcctgta tccgacgggc
     5521 cattaccacg cgttctctta taacagcact tttttcggag gcctgggctt tgctcagttc
     5581 tttgaagggt acgggagtaa cctccacatg gatgtcgatg cggtccagta aaggccccga
     5641 caccttgtta agatattttt gcacaatgcc cggtccgcag acacattctt tttccggatg
     5701 attatagtat ccgcagggac atggattcat ggccgccacc agcataaagc tggcagggta
     5761 ttccaccgaa aattttgccc ttgatattgt taccactcgg tcttccacgg gctgtcgcaa
     5821 cacttccagc accgttcttt taaattcggg cagctcgtca agaaacaaca cgccattatg
     5881 ggcaagagag atctcgcccg gttggggaaa actgccgccc cccaccaaag ccacgtcact
     5941 gatggtatga tgcggcgacc ggaaaggacg caccgaaatc agggaagcat tttttgctag
     6001 ttttccggaa acggaatg
//
LOCUS       DAOSFP010000115         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11693_length_5983_cov_4.893387, whole genome shotgun sequence.
ACCESSION   DAOSFP010000115 DAOSFP010000000
VERSION     DAOSFP010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11693_length_5983_cov_4.893387"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1511)
                     /locus_tag="PLI16_04595"
     CDS             complement(<1..1511)
                     /locus_tag="PLI16_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83869.1"
                     /translation="MTIKKLQSLCKNIFLLLGFCVTNSVYGQGYYNQWFFGEGAGLDF
                     NSCTPVTVAGNVYSIEGCASICDYYGNLLFYTDGVSVWNGNHALMPNGSGLLGHQNAA
                     QSSLIVPMPGDSNKYYIFTTPWYNDTGAIRYSIVDLSLDGGHGDVTATKNFMLHPVSE
                     ERVTAVRAANGTDFWILGHEPSNNVFFAYLLTAGGINPVPVLSTVGVTHPDIFASQGY
                     LKPNHHGDKLAMSVWFPHQLEIFDFDKTSGVISNPITFPTSLVSYGLEFSSDDNLFYF
                     SGLTACDLYQVNMNAGSDSLIIASTTLIASPPGPTCALLLGPDGKIYAAKYGVPYLGV
                     INNPNTQGYFCNYVDNQILLSTGISQSGLPNFFEMTVCTAPDVDLGNDTTLCAGQSLT
                     LSVGPANSYLWSDSTTGPSITVTAPGTYWVEACIAPCLARDSITVNFVPAAVDILTND
                     TAVCEGQSVMLVATGMSAYTWAPQESLNISSGDTVIAGPTSTTTYTVTGSSSG"
     gene            1662..1787
                     /locus_tag="PLI16_04600"
     CDS             1662..1787
                     /locus_tag="PLI16_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83870.1"
                     /translation="MFFYLCNKDFTLCGHKADACWAVLQGARKIKKLKRYDIPGS"
     gene            1855..2919
                     /locus_tag="PLI16_04605"
     CDS             1855..2919
                     /locus_tag="PLI16_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOH83871.1"
                     /translation="MSRKTVLTLIGILIITTVFGQTDSSLVYFTKDISAEGMLKVFSF
                     IEKEVSGKVAFKVHFGEEGNQNYLPAGMMKPLVKKLDATLVETNVLYVSKRHYTKSHI
                     QLAKDHGFTYAPIDILDSEKDTGIVTGTKHFDTIYTGSHFFNYDTYVICSHFKGHGSA
                     GFGGAIKNVSMGLAAIQGKRALHANDVPATHPNKCIQCGACLKECPSGAITLNPLTID
                     RSKCIGCGKCISTCPVQSFSAPRGSAKEIVFLEHLVEYAKVFTGQKKMVYINVLANIS
                     KSCDCAGTAPKPFLVDIGILASTDIVAIEKASHDMVDNACQCDDAFVKAGNPSGKHQI
                     DYAAELKMGNPVYKLITVDK"
     gene            complement(2937..3389)
                     /gene="dtd"
                     /locus_tag="PLI16_04610"
     CDS             complement(2937..3389)
                     /gene="dtd"
                     /locus_tag="PLI16_04610"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOH83872.1"
                     /translation="MRVVVQRVNEASVLINNTQRKSIGKGLLVLLGIEDSDWDEDIEW
                     LCGKVARLRIFNDNQGVMNLSVTDISGEVMVISQFTLHASTKKGNRPSYIRAAKPETA
                     IPLYEKFIRQMETEINKSITSGEFGAYMQVELENDGPVTIIIDSKDKE"
     gene            complement(3519..4334)
                     /locus_tag="PLI16_04615"
     CDS             complement(3519..4334)
                     /locus_tag="PLI16_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOH83873.1"
                     /translation="MKNKIIVPTDLTPIAGQAIKQAVAIALKTNFGLTLLHVLTEKAE
                     SKEEVTKKLLAEAENIYSHSGIRCDILVREGSIFDIIPYEACERNYDLMVIGTHGIKG
                     IKQMLFGANILKLAEKICIPLLVVQEKSQAIKSFKKIVLPVGSHDTFNDAVEAVLFFA
                     GVFDAEVHLYSIRKNGFDWPDSLINNIENAVRRFESRGIQLVRVKEDQNVYSLGYAKQ
                     TLKYAQSVGADLICTMSVPSKEYYYFADSDKENLLLNEYHIPILCAGGGKTEK"
     gene            4690..5211
                     /locus_tag="PLI16_04620"
     CDS             4690..5211
                     /locus_tag="PLI16_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83874.1"
                     /translation="MKKTQTSFKNKAALISIIRNTLVGGIIVCCVAAGNAQVTTTRAS
                     EGLSEQKATPKNQSVSTRPDITLQRIQPEATREKMGEHAPDFNDENFQTKKAEWIKNY
                     PEEYEAFLKKTATSGARKVQTPQAVKPHQKIAEHAPFLDDPDYAAKKAEWMKNYQQEY
                     EQVMQEGQHSKIK"
     gene            5255..>5983
                     /locus_tag="PLI16_04625"
     CDS             5255..>5983
                     /locus_tag="PLI16_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83875.1"
                     /translation="MKKTIRIIISAVYLFSAIVLSDKIQAQPYTQNKTISAQKDVLKS
                     SATNDFLNNKPASHTITSEPRGQLSFADRNWRGEGRYAAFTPEQEDLSKRDENSKHFL
                     KADGTLTAVISQGPVHYKDNAGNWQDIRTDIRPNTSGKMTDYPYAVTENKHQVYIPQT
                     LDEGYLVQFTNGGVKMGMQGEVRLLSADYTPLKSLTRASANGTINDNTMKYTGTYPFG
                     YDELTLGWMGVEHDIILNEKPALLD"
BASE COUNT         1844 a         1265 c         1239 g         1635 t
ORIGIN      
        1 catccgcttg acgatcccgt aaccgtataa gtggtagtgg aagtcggtcc ggctattaca
       61 gtatcccctg aactaatgtt taatgactcc tgcggagccc aggtataggc agacatgccc
      121 gttgcaacca gcataacgga ctgtccttcg caaacagccg tgtcattggt caggatatca
      181 acagcggcag gtacaaaatt cacagttatc gaatccctgg caaggcaggg agcaatacag
      241 gcttccaccc aataagttcc aggggcggta accgttattg acggtccggt ggtgctgtcg
      301 gaccacaggt acgaatttgc cggaccaaca gataaagtaa ggctttgtcc tgcacacagc
      361 gtggtatcat taccgaggtc aacatcaggt gcggtacaga cggtcatttc gaagaaattg
      421 ggcagccccg actgagaaat accagtagat aaaagaatct gattgtccac ataattacag
      481 aaatagccct gagtattggg attattgatt acgccgaggt agggcacacc atatttggcg
      541 gcataaattt taccatcggg acctaagagc agggcacagg tgggtcccgg agggctggct
      601 atcagggtag tagaggcaat gatcaggctg tcgctgccgg cattcatgtt tacctgatac
      661 aggtcgcaag ccgtgagacc actgaaataa aacaggttgt cgtcggaaga aaactccaat
      721 ccataagaaa caagactggt tggaaaagta atcggattag aaatcacccc ggaggtttta
      781 tcaaaatcaa aaatttcgag ttggtgggga aaccaaaccg acatggccag cttatcgcca
      841 tgatggttag gctttagata accctgactt gcaaagatat ccgggtgagt aacacccacc
      901 gtgcttaaca caggaacagg attaatgccg ccagcagtca gcaggtaggc aaaaaaaaca
      961 ttgtttgatg gttcatggcc gagtatccag aaatcggtac cattggctgc gcgcacagca
     1021 gtgacacgtt cttccgagac aggatgcagc ataaagtttt tggttgccgt tacatcgcca
     1081 tggcctccgt cgagagagag gtcaacaatg gaatatctta tggcgccggt atcattatac
     1141 cagggagtgg tgaaaatata atacttattg gaatcgccgg gcatgggtac gatcaacgac
     1201 gactgggcag cattctgatg ccccaatagc ccactgccat ttggcatcag cgcatgattg
     1261 ccgttccaga cacttactcc atccgtgtaa aaaagaaggt tgccataata atcgcatatt
     1321 gaagcacatc cttcaatcga atatacattc cccgccacgg ttacgggtgt gcatgaattg
     1381 aagtcaagac cggcaccttc accgaaaaac cattggttat aataaccctg accatatacg
     1441 gaatttgtaa cacaaaatcc taataaaaga aagatatttt tacaaagaga ttgcagtttt
     1501 tttatggtca taacaaaata attagtgaaa acaattacca acaatttgtg cttcctaaat
     1561 ttatttcttg tgatagagga tcactcattg cttatttatt cagttttttt tgcgaaaaaa
     1621 cagttttgag caaatgtata aaatatcctg taaaaaacac tatgtttttt tatctgtgca
     1681 acaaagattt tacgctctgc ggccataagg ctgatgcgtg ctgggcggta ttacagggag
     1741 ccagaaaaat aaaaaaattg aaacgatatg atatccccgg atcataattg ttccctgcat
     1801 attttgtcat caggctgcta agtaatattt ttgcacccaa ttaataaatt ttttatgtcg
     1861 agaaaaactg ttttaacctt aattggaatt ttaataatca ctacggtatt tggacaaaca
     1921 gattcgtcgt tggtttattt cactaaagac atcagtgctg aaggcatgct gaaagttttt
     1981 tcttttattg agaaagaagt cagcggcaag gtagctttta aagtgcattt tggcgaagaa
     2041 gggaatcaga actacctgcc tgccggaatg atgaagcccc tggtaaagaa gctggatgcc
     2101 accctggttg aaaccaatgt gctgtatgtc agcaagaggc actacaccaa gtcgcacata
     2161 caattggcca aagatcatgg ctttacctat gccccgattg atattcttga ttcggaaaaa
     2221 gatacgggta tcgttaccgg aacaaaacat tttgatacca tatacaccgg gtcgcatttt
     2281 tttaattatg atacgtatgt aatatgctcc cactttaaag gtcacggaag cgctggattt
     2341 ggcggggcca tcaaaaatgt cagtatggga ttggccgcta ttcagggcaa aagagcttta
     2401 catgccaatg atgttcctgc cactcatcct aacaagtgca tccagtgcgg ggcatgcctt
     2461 aaagagtgtc cgtcgggggc aattacacta aatccgttga ctattgacag aagcaagtgt
     2521 attggatgcg gaaaatgcat cagcacatgt ccggtacaat cattttcggc tccacgcgga
     2581 tcagcaaaag aaattgtttt tctggagcat cttgtagaat atgccaaagt gtttaccgga
     2641 caaaaaaaga tggtttatat caatgtactg gctaatattt caaaatcttg cgactgcgca
     2701 ggtactgccc ccaagccatt ccttgtggat atcgggattt tggcttctac ggatatagta
     2761 gctattgaaa aagccagcca cgacatggtg gataacgctt gccaatgtga tgatgctttt
     2821 gtaaaagccg gtaatcccag tggaaaacac cagatcgatt acgccgccga actcaaaatg
     2881 ggaaatccgg tatataagct gataactgtt gataaataag agtgtaacac tcctttttat
     2941 tctttatcct tcgaatcgat aattattgta acgggcccgt cgttttcaag ttccacttgc
     3001 atataggcgc caaattcgcc actggtaata cttttattta tttcggtttc catttgccgg
     3061 ataaactttt cgtatagagg gatggctgtc tcgggttttg ctgcacggat atatgatgga
     3121 cggttgccct tttttgtgct ggcatgcaag gtaaattgac taataaccat tacttctcct
     3181 gaaatatctg tgactgaaag gttcattacc ccctggttgt cgttgaaaat tcgcaaacgg
     3241 gcaactttcc cgcagagcca ttcgatatct tcatcccaat cggaatcttc tatgccgagc
     3301 aaaacaagca aacccttgcc gatgcttttt ctttgggtgt tgtttattaa aaccgaagcc
     3361 tcattaacgc gttgaacaac caccctcatc tttattacgt attaaatcag aaaaattcat
     3421 ttttttcggg gtatctttat ttccagacca agaccgttta cctgcctgac atttcatttc
     3481 agattcggta acaatttacc gatcggccat taacaatctt atttctctgt tttaccgcca
     3541 ccggcacaaa gtattggtat gtggtattca ttcaaaagca ggttttcttt gtcggagtct
     3601 gcaaaatagt aatattcttt ggatggcacc gacatggtgc atataaggtc ggcaccgaca
     3661 gattgtgcgt atttcaacgt ttgctttgca tatcccaggg agtaaacatt ttgatcttcc
     3721 ttcacacgaa ccaactggat gcctcttgat tcaaaacggc gaactgcgtt ttcgatattg
     3781 tttatcaggc tgtctggcca gtcaaagcca ttcttgcgta tagagtacag atgaacctcg
     3841 gcgtcgaaaa caccggcaaa aaacaatacg gcctccaccg catcattgaa agtatcgtgg
     3901 ctgcccaccg gtaaaacaat ttttttgaaa gattttattg cctgcgattt ttcctgtact
     3961 accaacaagg gtatgcaaat tttttctgcc agtttaagaa tgtttgcacc gaacagcatt
     4021 tgtttgattc ctttgatgcc gtgtgttcct ataaccatta ggtcataatt gcgttcgcat
     4081 gcctcataag gaataatgtc aaaaatagat ccttcgcgaa ccaaaatatc acaacggata
     4141 ccgctatggg aataaatatt ttccgcttct gccaacagtt ttttcgtcac ctcttctttg
     4201 gattctgctt tttcggtaag cacgtgcagc agtgtcaggc cgaaattggt tttcagcgca
     4261 atagcaacgg cctgctttat tgcctggcct gcaattggag ttaaatcggt aggtacaatt
     4321 attttatttt tcattttctt attatcaaca ttgttattag caaacttacg gaatattcat
     4381 gaaataaata ttcacaaaaa aataaaaagt atttatcatg caactaaaat tttacaaaga
     4441 gttttgccat attgctacat ttccgatttc tcccggataa ttaattaaca ttcgcctaaa
     4501 attaaaaatt gaaggtagaa atatttttat caggtaaatg ggaaaataca aataataaat
     4561 acggtatatt tcaaattaac aatagtatat tggtaaaaat tgtaatattg cagacttttt
     4621 tcagactttt ttaaatttaa aacaaattcg aaaggtcaaa aaaccaaatt aatcaataaa
     4681 aaccaagata tgaaaaaaac acagactagt tttaaaaaca aggcagccct gatttcgatt
     4741 atccgcaata cattagtagg cggaataatt gtttgttgtg tcgctgccgg aaatgcgcag
     4801 gttactacaa cacgcgccag tgaaggactt tctgagcaga aagcaacccc aaagaatcaa
     4861 tccgtcagca ccaggcctga tatcactttg caaaggatac aaccggaagc aacccgggaa
     4921 aaaatgggcg aacacgctcc ggattttaac gatgaaaatt tccagaccaa aaaggcggaa
     4981 tggataaaaa attatcctga agaatacgaa gcatttctga aaaaaactgc aacttccgga
     5041 gctcggaaag ttcaaacacc acaagctgta aaaccccatc aaaaaattgc agaacatgct
     5101 ccttttcttg acgatcctga ttatgcagcc aaaaaagccg aatggatgaa aaattatcag
     5161 caggagtacg aacaggtaat gcaagaaggc cagcattcca aaataaaata ataaccaaga
     5221 ggttcatgtc taaccaacga taataaaacc gacaatgaaa aaaaccatac gcataattat
     5281 ttcggccgtt tatctttttt cggcaattgt tttgtctgat aaaattcagg cacaacctta
     5341 cacacagaac aaaaccataa gtgctcaaaa agatgtttta aaatcatcag ccaccaatga
     5401 ctttttaaat aataagcctg catcccatac tattacgtca gaaccccgtg ggcaactttc
     5461 ttttgcggat cgcaactggc gcggcgaagg gcggtatgct gcctttacac cagaacagga
     5521 agacctttcc aaacgcgacg aaaactccaa acattttttg aaagctgacg gcacgctcac
     5581 cgcggttatt tcgcaggggc ctgtacatta caaggataat gccggcaact ggcaggatat
     5641 cagaacggat atccggccaa atacttctgg aaaaatgact gattatccat acgctgtaac
     5701 cgaaaacaaa catcaggttt atattccgca aacccttgat gaaggatact tagtgcaatt
     5761 taccaacggt ggagtcaaga tggggatgca gggtgaagtg cgcttgcttt cggcagatta
     5821 tactcccttg aaatccctta cccgtgcttc ggccaacggc accatcaacg ataacaccat
     5881 gaaatatact ggcacatatc cttttggata cgatgagttg actttaggct ggatgggtgt
     5941 cgaacacgat attattttaa acgaaaaacc tgcactgctc gac
//
LOCUS       DAOSFP010000116         5968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11726_length_5968_cov_4.262303, whole genome shotgun sequence.
ACCESSION   DAOSFP010000116 DAOSFP010000000
VERSION     DAOSFP010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11726_length_5968_cov_4.262303"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLI16_04630"
     CDS             complement(<1..248)
                     /locus_tag="PLI16_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH83876.1"
                     /translation="MKELSININYKELASSDELDVADAYLLARATEACNNAYAPYSLF
                     QVGAALLLENGEIVCGNNQENAAYPSGLCAERVAMFAAS"
     gene            complement(266..1846)
                     /locus_tag="PLI16_04635"
     CDS             complement(266..1846)
                     /locus_tag="PLI16_04635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOH83877.1"
                     /translation="MRLQRSDIHRYIYILGLTGMAMSLTLSLYVMSVAQFVLLANWLA
                     EGRLAAKFKAFFHNKAALVFTLLYLLHVVGLFCTHDFETALAELRIKLPLLALPVILS
                     TSPALSGKTLNNILLAHIAGTFISGVYSFYLFHSGPVQDIRDIFIISHIRYSLNVCTD
                     IFILLYFIFYKNNYKTGIKIFFSVLSAWFIYYLFFMESFTGMVLLITTLAVVLVYFVF
                     KQSKPVYKISLLAFFMVMVSGSALYVHHIYEKYINIDSVNPQKLEQFTRHGNPYVHDL
                     KNPMTDNGHYTWIYVCESELKEAWDKRSKIKYGENDAADSPVKYTLVRYLTSKGLKKD
                     SYGVNSLSDEEIKLIEQGVANVDDAKKSNFENRLKNLLWEIEIYKFTRDPRQQTLMQR
                     VELWRTSARLIKDHFWFGLGTGDVKNVFAAKLELDDSLLKNSGLRPHNQYLTIMLEFG
                     VFGLLLFLFCLFYPSLRQKNFFDFLYLTFFIVVVFSMLTEDTFETQAGVTYFAYFSCL
                     LLFVQKNALSENKTLILE"
     gene            complement(1890..3578)
                     /locus_tag="PLI16_04640"
     CDS             complement(1890..3578)
                     /locus_tag="PLI16_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOH83878.1"
                     /translation="MKKIKILFLTLTLLSVPALVGRAQDQVQNDFEISKNIDIFVSLY
                     KQLNTYYVDDLNPGQLMKTAMDAMLKSLDPYTVYIPESDIEDYKFMTTGQYGGIGALI
                     HKRGNYVFISEPYENSPADKAGLKAGDKILEINGKTARGKTVDEVSTALKGQPGTNVS
                     LLLERDSAQAAFTKEITRQEIKIDNVPYYGMVADSIGYINLSEFKQNAGNDVKNAFVK
                     LNENKKLKGVIIDLRGNGGGLLNEAVNIVNIFMDKGQFVVSTKGRIKDQNHIHKTQGA
                     PVDTEIPLVILVDGGSASASEILAGSIQDLDRGIIIGQRTFGKGLVQNIIPLSYNSEM
                     KITIAKYYIPSGRCVQAMDYFNKDEDGNAPVLPDSLRKAFTTKNGRVVYEGSGIEPDI
                     IEEIPELSNIALALIEKNHFFDFAVYFYRKTPSIAPAADFVVTDEIYNDFISYLKNKD
                     YSYSTESELVLDDLKKAAETEKYYDAIKNDFDLLKEKITKEKSNDLTANKEQIKQMMK
                     MYIIPLYYYQKGRIESSLKDDPDVMKAIEILKDTSRYKSILNGTHSETGKKSKK"
     gene            complement(3593..3814)
                     /gene="yidD"
                     /locus_tag="PLI16_04645"
     CDS             complement(3593..3814)
                     /gene="yidD"
                     /locus_tag="PLI16_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOH83879.1"
                     /translation="MKKYIGKFFILLIKIYQYGISPYLMPSCRYTPSCSAYGVEAIQK
                     YGPFKGGWLTLKRILSCNPWGGSGYDPVP"
     gene            complement(3811..4185)
                     /locus_tag="PLI16_04650"
     CDS             complement(3811..4185)
                     /locus_tag="PLI16_04650"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOH83880.1"
                     /translation="MPSFCKDERLCGKKSISLLMAEGKKYFQYPFAVKWIDLPDTQQP
                     PVQLLVVVAKRHFKKAVDRNKIKRYVREAYRNNKELLSVSLKEKNKHIALMLSYSSEK
                     TSAYREIEAKVQLILKYLAQNI"
     gene            complement(4203..4955)
                     /locus_tag="PLI16_04655"
     CDS             complement(4203..4955)
                     /locus_tag="PLI16_04655"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOH83881.1"
                     /translation="MKVKNIIISQPQPPNYEKSPYYDLAKKHNLNILFKKFICIEPVS
                     AQEFRREKINILDHTAIIFTCNNAVDHFFRLAKEMRLTIPDTTKYFCVSEKTAFYLQK
                     YVQFRKRKIFNGKEKPEELITIIKKHLQEKFLLPCTEGHKQEIVNLLQTNKIPVRKAM
                     IYRTIPNMEVKQDINIAKTDMLVFFSPFGIKALFENFPDFKQNKMVIATWGNTTFNAA
                     IDAGLNISVFGPSQSFTSMPMAIDAFLDKVKK"
     gene            complement(4988..>5968)
                     /locus_tag="PLI16_04660"
     CDS             complement(4988..>5968)
                     /locus_tag="PLI16_04660"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOH83882.1"
                     /translation="SNMLAKMCNPISPYFANFAQFMVGDSLTYNVNALPQENASFFKT
                     FIRSQDEVSAQLGKTCFVGHSKDNLPATYVKQTSSKSDTAYCTSVFYTGQDPVIQIVP
                     KEIPVRHSDSFFYLLLFCLVLGTLILFVKHRRISQLFKAFYLPYFTNQLMREGLIQRE
                     FFSFPLLLVYYISLSMFIAQGLHFFYQMEIDFYFVLEILGVTVALFLFRSFLIILIKI
                     AFKTPGETAEYNTNNFLFSIITGIFLTPMVFVIHYMNAPGAKILFFAVLFITSLLFLY
                     RLVRSFLIGMSNEGHRLYYFILYLCTIEILPLVICVKLLVNIYFKGVLIV"
BASE COUNT         1750 a         1219 c         1224 g         1775 t
ORIGIN      
        1 gatgctgcaa acatggccac ccgttcggca cacaatcccg acgggtaggc agcattttcc
       61 tggttgttgc cacaaactat ctcaccgttt tcgagcagca gggctgcgcc cacctgaaat
      121 aacgaataag gggcataggc attattacag gcttcggtag ccctagccaa caaataagca
      181 tcagccacat caagctcatc cgatgatgct aactccttat aattgatatt tatcgaaagc
      241 tctttcatgg gttgtttcct gaaatttact ccaaaattag tgttttattt tcagacaggg
      301 catttttctg cacaaaaagg agcaagcaac taaaataggc aaaataagtt acgccggcct
      361 gagtctcaaa agtatcttct gtgagcatgg aaaatactac gacaataaaa aatgtcaggt
      421 acagaaaatc aaaaaagttc ttttgtctta aagacggata aaacaagcaa aaaagaaata
      481 acaacaatcc aaacactcca aactccaaca ttatagtaag gtactgatta tgtggacgaa
      541 gcccggaatt tttcagcaag gaatcgtcca gttcgagttt ggcggcaaat acatttttca
      601 catcgccggt gcccagtcca aaccaaaaat ggtctttgat aagacgcgcc gaggtgcgcc
      661 ataactccac ccgctgcatc aacgtttgtt gccgcggatc gcgggtaaac ttatatattt
      721 ctatttccca taacaagttt ttcagcctgt tctcaaagtt gctttttttt gcgtcatcaa
      781 cattggccac cccttgttca atgagtttaa tttcttcatc ggaaaggctg ttaacgccgt
      841 agctgtcttt tttcaacccc tttgaggtaa ggtaacgcac cagagtatat ttcactggtg
      901 aatcagctgc atcattttct ccatacttta ttttactccg tttatcccag gcctctttca
      961 gttcggattc acacacataa atccaggtat aatgtccatt gtcggtcatg ggatttttca
     1021 ggtcatggac gtacggatta ccatgcctag taaattgttc aagtttttgg gggttgacag
     1081 agtcaatatt tatatacttt tcataaatat ggtgaacata cagagcgctg ccggatacca
     1141 tcaccatgaa aaaggccagc agggatattt tatacacagg ctttgattgt ttaaaaacaa
     1201 aataaaccag cacgacggcc agcgtggtaa tcagcagtac catcccggta aacgactcca
     1261 taaagaaaag ataataaata aaccaggctg acaggacaga aaaaaatatc tttatccctg
     1321 ttttgtaatt gtttttgtaa aaaataaaat agagcagaat aaaaatgtcg gtacaaacat
     1381 taagcgaata ccggatatgg gaaattataa aaatatcgcg gatatcctgc acagggccgg
     1441 aatggaacaa atagaaactg tagactcccg aaataaaggt tccggcaatg tgggcaagca
     1501 aaatattgtt tagcgttttt cccgacaagg ccgggctggt agatagaatt acgggcagcg
     1561 ccagcagggg cagtttgatg cgcagctccg cgagtgcagt ttcaaaatca tgggtacaaa
     1621 acagaccaac aacatggagt aaataaagca aggtgaatac cagggcggct ttgttatgaa
     1681 aaaaagcttt gaatttagcg gccagcctgc cctcagccaa ccagttggcc agaagcacaa
     1741 actgggcgac gctcatgaca taaagcgaca aagtcagcga catagccatg ccggtaagcc
     1801 ccaaaatgta aatatacctg tgaatgtcag atctctgtag ccgcataact cacaaaagaa
     1861 aaatttctat gccggaaagg tccggatttc tattttttgc tttttttccc tgtttcgcta
     1921 tgggtgccat taaggataga tttatatcgg gaagtatctt ttagtatttc gatggccttc
     1981 atcacatcgg gatcatcctt cagcgacgat tcaatacgac ctttctgata ataatacagc
     2041 gggatgatat acattttcat catttgtttt atctgctcct tgttcgcagt caggtcgttg
     2101 cttttttctt ttgtaatttt ttctttcagc agatcaaaat cattttttat ggcatcataa
     2161 tatttttcgg tttcggctgc ttttttcaga tcatccagca ccagttcgct ttccgtcgaa
     2221 taggagtaat ctttattttt caggtagctg ataaaatcat tataaatctc gtccgtaacc
     2281 acaaaatcgg ctgccggggc aatggagggt gtctttcggt aaaaataaac ggcaaagtcg
     2341 aaaaaatgat ttttttctat cagggccagg gctatattgc ttaattcggg tatttcctca
     2401 ataatatccg gttctattcc gctgccttca tacactaccc tgccattttt tgtagtaaat
     2461 gctttgcgca gcgaatcggg cagtacggga gcgtttccgt cttcgtcttt attaaaatag
     2521 tccatcgcct gaacacaacg gccgctggga atataatact tggcaatggt aattttcatc
     2581 tccgaattgt acgacagggg gataatattt tgtaccaggc cttttccgaa agtccgttga
     2641 ccaataataa tgccccggtc gagatcctga atggatcctg caaggatttc tgatgcagaa
     2701 gccgagcctc catccaccag gataaccaga ggaatttcgg tatcaacggg tgctccctga
     2761 gttttgtgaa tatgattctg gtctttgatt cttcccttgg tgctgacaac aaactgcccc
     2821 ttatccataa aaatattgac aatgttcact gcttcattga gcagtccgcc gccattgccc
     2881 ctcaggtcga tgatgacgcc tttgagtttt ttgttctcgt tgagtttaac aaaagcattt
     2941 tttacgtcat taccggcgtt ttgtttgaac tcgctcagat taatgtaacc gatgctgtcg
     3001 gccaccattc cgtaataggg gacattatca atttttattt cctgtctggt aatttctttg
     3061 gtgaaagctg cctgtgcaga atcgcgttcg agcaacaaag aaacattggt tcccggctgg
     3121 cctttcagag cggtgctaac ttcgtccacg gtttttccgc gggcagtttt tccgttgatt
     3181 tcaagtattt tatctccggc tttcaatccg gctttgtcgg caggtgaatt ttcataaggt
     3241 tccgagataa acacataatt acccctttta tgtatcaagg cgccaatgcc gccatactgc
     3301 ccggtggtca taaacttata atcttctata tccgattcgg ggatgtacac cgtgtaaggg
     3361 tcgagggatt tcagcatagc atccatggct gttttcatca actgtcccgg attcaggtca
     3421 tcaacataat aggtattgag ttgcttataa agagagacaa atatgtcgat gtttttggaa
     3481 atctcaaaat cgttctgaac ctgatcctgc gcccggccca ccaatgccgg cacagatagc
     3541 agtgtcagcg ttaaaaaaag tatttttatt ttcttcattt tatatttatc aattacggga
     3601 ccgggtcata gccgctgccg ccccaggggt tacacgaaag gattcttttc aaggtaagcc
     3661 atccgccctt gaatggccca tatttttgta ttgcttctac tccataagcg gaacacgatg
     3721 gtgtgtaacg gcacgaaggc atcaggtaag gtgatattcc gtattgataa attttaatca
     3781 acagaataaa aaattttccg atatactttt tcatatgttt tgtgccagat actttaaaat
     3841 taactgaact tttgcttcta tttcccggta agcggatgtt ttttcactgc tgtaagaaag
     3901 catcaacgca atatgtttat ttttttcttt caaagaaacg gacaacaact ccttattatt
     3961 gcgataggct tctctgacat acctttttat tttgttgcgg tccacggctt ttttaaaatg
     4021 tcttttggca accaccacca gcaactgtac cggtggctgc tgagtgtccg gcaggtcaat
     4081 ccatttaacg gcaaagggat actgaaaata tttttttcct tctgccatca ggaggcttat
     4141 gctttttttt ccgcacaagc gttcatcttt gcaaaaggag ggcattgtcg ggaggtttct
     4201 gattattttt tcaccttatc gagaaaggcg tcaatagcca tcggcataga ggtaaaactt
     4261 tgcgagggtc cgaaaacact tatattcagg cctgcatcga tagcggcgtt aaaagttgta
     4321 tttccccagg tagcaattac cattttgttt tgtttaaaat ccgggaagtt ttcgaaaagc
     4381 gctttgattc cgaacggact gaaaaacacc agcatatctg tcttggcaat gttgatatcc
     4441 tgtttgactt ccatattggg gatggtgcgg taaatcatgg ccttacgaac gggtatcttg
     4501 ttggtctgaa gcaggttgac gatttcctgt ttgtggcctt ccgtgcaggg taaaagaaat
     4561 ttttcctgca aatgtttttt tatgatggta atgagttctt cgggtttttc tttgccgttg
     4621 aagatttttc tcttccggaa ctgcacatat ttttgaagat aaaaagccgt tttttcggag
     4681 acacaaaaat atttggtggt atcgggaatg gtcaggcgca tttctttggc cagcctgaaa
     4741 aaatgatcta cggcattgtt gcaggtaaaa attatggccg tatgatccaa aatattgatt
     4801 ttttcccttc tgaactcctg agctgaaacg ggctcaatac agataaactt cttaaatagg
     4861 atgttaagat tatgcttttt cgccaaatcg taatagggag atttttcgta attgggaggt
     4921 tgcggttgcg aaattattat gtttttaact ttcaatgcgg tgttttttaa gttaataaat
     4981 aattttctca gacaatcaaa acccctttaa aataaatgtt aaccaacagt ttaacgcaaa
     5041 taacaagggg caaaatttcg atggtgcaaa ggtacaaaat aaaataatac aaacgatgtc
     5101 cttcgtttga catcccgatt aaaaaacttc ggacaagccg gtataaaaac aacagggagg
     5161 tgataaacaa aaccgcaaaa aagaggattt tggcgccagg ggcattcata tagtgtatca
     5221 caaataccat aggcgtcaaa aatatgccgg taataattga aaaaagaaaa ttattggtat
     5281 tatactctgc ggtttccccg ggggttttga aagcaatttt tatcaatatg atcagaaaac
     5341 tcctgaacaa aaataatgcg acggtaacac ccaaaatctc caaaacaaaa taaaagtcta
     5401 tttccatctg ataaaaaaaa tgcagtcctt gcgctataaa catggaaagg gaaatataat
     5461 agaccaacag caggggaaaa gaaaaaaact cacgttgtat cagcccttcc cgcataagct
     5521 gattggtaaa atacggcagg taaaaggctt taaataattg agaaattcgt ctgtgtttca
     5581 caaaaagaat tagcgttccc aaaacaagac aaaacagcaa cagataaaaa aacgaatcac
     5641 tgtgcctgac gggaatttct ttaggcacaa tttgtatcac ggggtcctgt cctgtgtaaa
     5701 aaaccgaggt gcaataagca gtatccgatt ttgacgaagt ttgttttaca tatgttgctg
     5761 gcaggttgtc tttggaatgg cccacaaaac aggttttgcc taattgtgcg gatacctcat
     5821 cctgtgagcg gataaatgtt ttaaaaaacg aggcgttttc ctgcggcaga gcattgacat
     5881 tatatgtaag actatctcca accataaatt gtgcaaaatt agcaaaatat ggcgaaattg
     5941 ggttgcacat ttttgccagc atatttga
//
LOCUS       DAOSFP010000117         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11769_length_5950_cov_3.832909, whole genome shotgun sequence.
ACCESSION   DAOSFP010000117 DAOSFP010000000
VERSION     DAOSFP010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11769_length_5950_cov_3.832909"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /gene="rsgA"
                     /locus_tag="PLI16_04665"
     CDS             complement(<1..668)
                     /gene="rsgA"
                     /locus_tag="PLI16_04665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOH83883.1"
                     /translation="MTTLKGIVIKSTGSWHTVRSSEAKEYLCKLKGQYRIRGLKATNP
                     LTVGDHVEFYLSGAEEVGIIDKILPRKNYIIRKSKKLSKQVHIIAANIDMAYLVVTPV
                     QPRTSTGFIDRFLITAEAYHIPATLIFNKIDLYDSGLWQQHQDLVRIYTGAGYPCIEV
                     SALRGDNIDVLRDKLKDTINLFSGHSGVGKSALINAVDPTKNLKTGAISEMFLKGKHT
                     TTFT"
     gene            complement(665..934)
                     /locus_tag="PLI16_04670"
     CDS             complement(665..934)
                     /locus_tag="PLI16_04670"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH83884.1"
                     /translation="MQQITIQQLSEEEIQKRNIRSWPVWTKEISRFDWYYDSEEECLI
                     LEGEITVETADNKYTISAGDFVTFKKGLRCVWNVTKPVRKHYNFK"
     gene            1036..1815
                     /locus_tag="PLI16_04675"
     CDS             1036..1815
                     /locus_tag="PLI16_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83885.1"
                     /translation="MKKSLLYFVFSVLFVFMTGFAFGQCTPDPTVTDPEGDGRMDPDT
                     IEATVNVPTNVTATIICPDTASVGVAGHINLHHIKVKSLLNQPAWLSYVCNPSNCEFP
                     AGEAKCVLVTGTPPAGTTPGYYLIDVIVDVYSQPIVPGGQPILAVSDFNSGMQLVVWV
                     HEEGYNVAEFEHKGFGLLAPQPNPFRSTTKLGCFTEKAQTVSLRIFDMVGKEVYNEKL
                     NTSAGENYFVFDGSNLNDGVYFYTVIDAQNRVITKKMIKSR"
     gene            complement(1895..2596)
                     /gene="tsaB"
                     /locus_tag="PLI16_04680"
     CDS             complement(1895..2596)
                     /gene="tsaB"
                     /locus_tag="PLI16_04680"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOH83886.1"
                     /translation="MALILHIETATNICSVALSDKEEIIGLKETGKHNAHSEMLTIFV
                     RDVLSDAGTSPAALDAVSVSSGPGSYTGLRIGVSVAKGLCYALDKPLISVGTLAAMAY
                     GALEKKDIGQKVVWLCPMLDARRMEVYCAIYNNMMQEVQPPSAKIFDAHTFTEVPPDQ
                     PLILFGNGMDKCRHLFEGNPMISFLDHYEVSARNMVLPALAKFYAQDFENVAYFEPHY
                     LKDFIAGKPNVKGLK"
     gene            complement(2609..3982)
                     /locus_tag="PLI16_04685"
     CDS             complement(2609..3982)
                     /locus_tag="PLI16_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH83887.1"
                     /translation="MKKKKIIRFSLIGAVVIIILLAVGKKTGCIGKSNAINVATEKVI
                     KRTIVESVSASGKIQPEVEVKLSPDVSGEVVELLVKEGDKVSKGDLLAKIKPDIYESN
                     YEQMQAALNSQKASEANAKARLAQVKSQFLNARLTYERNETLFKQKVISQAEFDAAKT
                     TFDVAQEEVTAAEELYKAAQYNVQSTNATVKEARENLAKTNIYAPVDGTISKLNIELG
                     ERVVGTTQFAGTEIMRIANLNEMEVSVEVNENDIVRVSLGDTAIIEVDAYQNRKFKGI
                     VTEIANTAESTGTITVDQVTNFTVKIRILRESYLDLVRPEHPNLSPFRPGMSATVDIQ
                     TETVHNVLSLPIQAVTTREDTSGRVFTAGKKTDEENDDSGEKDKKTEHSTKQQEYVFL
                     FTDGKAKMVKVTSGIQDNMYIQIVTGLKENDEVITSPYTAITKTLKDDAAVKKVDKKE
                     LFKEEKK"
     gene            complement(3992..5413)
                     /locus_tag="PLI16_04690"
     CDS             complement(3992..5413)
                     /locus_tag="PLI16_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH83888.1"
                     /translation="MKKFFTAVFLLFLWCCSHVFSQKIWTLEDCISHALENNIQVKQQ
                     QLNAALSKLQYNQSIAELFPSVNATANHVYNNGQTVDMYTNTFASQTVQSNNFYLSSN
                     VVLFSGLRLLNGLKQKQYDFLASSYDLEKMKNDISLTIATAYLQILYNLELLETSKNQ
                     LELSRQQLKRTEALFDVGTVAKGTVLQMEAQAVADELQVVTAQNQLDLSYLSLVQFLD
                     LQTTQGFDIEKPVFSVPETSLLSQQVEEIYEKACSIQPDVKSAEMKVQSAKKGLQFSR
                     GMMSPSLMLSGSYGTGYSGASKSIADISLSGYETIGATESGELVYGPTFDYTYEKVKF
                     WDQLENNLNSSVGLSLNIPIFNKWQTNTMIGTSKIAVSSAELSLQNTKNQLFKTIQQA
                     YADARGALNKYHASLKSVEALSESFNYTQQKFDAGMVNTLDYNDAKNKLAAANSEMLK
                     AKYEYVFRIKVIDFYQGKPLTLK"
     gene            5541..>5950
                     /locus_tag="PLI16_04695"
     CDS             5541..>5950
                     /locus_tag="PLI16_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83889.1"
                     /translation="MKKLITGLLLLFYIATASYAAAPAKAISDTIDVLNYCINVNLSH
                     ISTKSLSGSATLEFIPRHDNVSQVKLYLLKLNVDSIFYENNLLTTFSYNDTIIEINLP
                     QPIHLNDTVSLTVFYHGMPQTDASGWGGFYFSAD"
BASE COUNT         1531 a         1360 c         1253 g         1806 t
ORIGIN      
        1 tcggtgaatg tggtggtatg tttgcctttc agaaacattt cggaaatagc gccggttttg
       61 aggttcttgg tcggatcaac ggcattgatc agggcgcttt tgcccacacc cgaatgtccc
      121 gaaaaaagat tgatggtatc tttcagctta tcacgcaaca catcaatatt atcgccgcga
      181 agcgccgaga cttctatgca tgggtagccg gccccggtat atattctcac taaatcctga
      241 tgttgttgcc agaggccgct atcataaagg tcaattttat taaaaatcag ggtggccggg
      301 atgtggtaag cctcggctgt gattagaaaa cggtcgataa agccggtaga tgtacgaggc
      361 tggacagggg ttaccacaag gtaggccata tcgatattgg ccgcaataat atgtacctgt
      421 tttgaaagtt tttttgattt gcgaatgata tagtttttac ggggcaggat tttatcaata
      481 atgcctacct cttcggctcc ggagagataa aattccacat gatcaccaac tgtcagcgga
      541 ttggtggctt tcaaccccct gatgcggtat tggcctttca gcttacatag atattcttta
      601 gcctcgctgc tccttacggt gtgccagcta ccggttgatt taatgacaat tccttttaat
      661 gttgtcattt aaaattgtaa tgttttctga caggctttgt tacattccac acacatcgta
      721 atcctttttt aaaggtcaca aaatcgccgg cactaatggt atatttgttg tccgcggttt
      781 caactgttat ttcaccctcc agaatcaggc attcctcctc gctgtcgtaa taccagtcga
      841 aacgcgaaat ttcttttgtc cagacaggcc aggaccgaat atttcttttt tgtatttctt
      901 cttctgaaag ttgttggatg gttatttgtt gcatgaattt cagatggtta cataatttag
      961 gatcaacaaa aaggccgtta ttgatttttc aatagtattt cgtatctttg caccaaaatt
     1021 aataaaatta acattatgaa aaaatcatta ctttattttg tgttttcagt tttgtttgtc
     1081 tttatgacag gctttgcctt cggccagtgt actcccgatc ctaccgttac cgatcctgaa
     1141 ggtgatggca gaatggatcc cgatactatc gaagctacgg taaatgttcc tacgaacgtt
     1201 accgccacta ttatttgtcc cgataccgct tctgtaggcg ttgcaggaca cattaacctc
     1261 caccacataa aagtaaaaag cctgcttaac caacccgcat ggttgtcgta tgtgtgcaat
     1321 ccctccaatt gtgaatttcc tgccggagaa gccaaatgcg tgttggttac cggaactcct
     1381 cctgccggaa caactcctgg atactacctg atagacgtta tcgttgatgt ttattctcag
     1441 cccattgtcc cgggaggtca gcctatttta gccgtgtcgg attttaacag cggtatgcag
     1501 ttggttgtgt gggtgcacga agaaggctac aacgtggcag aatttgaaca caaaggcttt
     1561 ggcctgcttg ctcctcagcc caatcccttc aggtcaacta ctaaactcgg ctgtttcacc
     1621 gaaaaagcac aaaccgtttc attaagaatt tttgatatgg ttggaaaaga agtgtataat
     1681 gaaaaactca atacttcagc cggtgaaaac tattttgtat ttgatggcag caacctgaat
     1741 gacggcgtgt atttttatac agtaatcgac gctcaaaatc gcgtaattac caaaaaaatg
     1801 atcaaatcaa gatagttgta caaaacactc aaaaaaagcg gcccagacgg agccgctttt
     1861 tttatgggta taataccgaa tgtatgctgt tgaactattt caagcccttg acattcggtt
     1921 tgccagcaat aaaatctttg aggtaatgtg gctcgaaata ggccacattt tcaaaatcct
     1981 gagcataaaa tttcgcgagg gcgggcagaa ccatattcct ggccgaaact tcgtaatggt
     2041 caagaaagct tatcatgggg ttgccttcaa acagatgcct gcacttgtcc atgccattgc
     2101 caaaaagtat caacggctga tcgggcggca cctccgtaaa agtatgagca tcgaaaattt
     2161 ttgctgaagg cggctgcact tcctgcatca tgttattata tatggcacaa taaacctcca
     2221 tacgccgggc atcgagcata ggacaaagcc aaacgacttt ttgaccaata tctttttttt
     2281 ctaaggcgcc gtaagccatg gcagctaatg tgcccacgct tatcaggggc ttgtctaaag
     2341 cataacacaa gcccttggcg acagaaacgc caatgcgcaa accggtatag gagccaggcc
     2401 ccgaactgac actcacggca tccagagctg caggagatgt gccggcatcg gacaacacat
     2461 cccgtacaaa aatggtgagc atttcagaat gtgcattgtg ctttccggtc tcttttaaac
     2521 caataatttc ttccttgtct gacaaagcga cagaacaaat attcgtcgca gtttcaatat
     2581 gtagaattaa tgccacagga gtctgccgtt attttttctc ttctttaaac aactcttttt
     2641 tatccacttt tttcaccgcg gcgtcgtcct taagggtttt tgtgatcgct gtatagggcg
     2701 atgtaattac ttcgtcattc tctttcaggc cggtaacaat ttgtatatac atattgtcct
     2761 gtattcctga agttactttt accattttcg ccttaccgtc ggtgaataaa aaaacatatt
     2821 cctgctgttt tgtagaatgt tctgtcttct tatccttttc cccggagtca tcattttcct
     2881 cgtctgtttt tttacccgca gtaaaaaccc tgccactggt atcttccctg gtagtaacag
     2941 cctgtatggg caacgacagc acattgtgta cagtttcggt ttgtatgtca acagtggccg
     3001 acattcccgg cctgaaaggc gaaagattgg ggtgttcggg gcgtaccagg tccaggtagc
     3061 tttcgcgcag gatgcggatt tttactgtaa aattggttac ctggtccact gtaatggtgc
     3121 cggtgctttc ggcagtgttg gctatttcgg taacgatacc tttaaatttc cggttctgat
     3181 aagcatccac ctcaatgatg gcggtatctc caaggctaac ccggacaatg tcgttttcgt
     3241 ttacttccac gctaacctcc atctcattca ggttggcaat gcgcataatt tccgtgccgg
     3301 caaattgggt ggtacccacc acgcgttcgc ccagttcgat gttcagcttt gaaatagtac
     3361 cgtccacagg cgcataaata tttgttttcg ccaggttttc gcgggcttcc tttaccgtag
     3421 cattggtgct ctgtacgttg tactgtgcgg ctttgtacaa ttcttctgcc gcggtaactt
     3481 cctcctgcgc cacgtcaaaa gttgttttgg ctgcatcaaa ctctgcctgc gaaataactt
     3541 tttgtttgaa cagagtttca ttgcgttcgt aagtaagccg ggcattgaga aattgcgatt
     3601 ttacctgtgc cagtctggct ttggcgttgg cctcgctggc tttctgcgaa ttaagcgccg
     3661 cctgcatctg ctcataattg gattcgtaga tatcgggttt tatctttgcc agcaaatcgc
     3721 ctttagatac tttgtcgccc tcttttacca gaagttccac cacttcgccc gagacatcag
     3781 gcgaaagttt tacttccact tccggctgta ttttgccgct agccgaaaca gactcaacga
     3841 tggtcctctt gatgactttt tccgtagcca cattaatagc attgcttttt ccgatgcagc
     3901 ctgttttttt gcccacagcc aacaggataa tgatgactac cgcgccaatt aatgaaaaac
     3961 gtatgatctt ctttttcttc atagaaaaat attatttaag ggtaaggggt ttcccctgat
     4021 aaaagtctat cacttttatc ctgaaaacat attcatattt tgcctttagc atttccgagt
     4081 ttgcggcagc gagtttgttt ttagcatcgt tgtaatccag agtgtttacc atccctgcat
     4141 caaatttttg ctgtgtataa ttaaatgatt cgctgagtgc ttcaactgat ttgaggctgg
     4201 cgtggtactt gttgagagcg ccgcgggcat cggcataggc ctgttgaatg gttttaaaca
     4261 actggttttt tgtattctga agcgagagtt cagcgctgct tacagcaatt ttggatgtgc
     4321 ctatcatcgt attggtttgc catttgttaa aaatgggaat gttgagcgat aagcccacag
     4381 agctgttcag attgttttcg agctggtccc agaacttaac tttttcatag gtataatcaa
     4441 aagtaggtcc gtaaaccagt tctcccgatt cagtggcccc gatagtttca tagcccgaca
     4501 ggctgatgtc cgcaatgctt ttgctggcgc ctgaataccc tgtgccgtac gagccgctga
     4561 gcatgagcga cggactcatc atgccccgtg aaaactgcaa accctttttg gcgctttgta
     4621 ctttcatttc cgcacttttg acatcgggtt gaatagagca ggccttttca taaatctcct
     4681 ctacctgctg tgataacaaa cttgtctcag gcaccgaaaa tacaggcttt tcaatatcaa
     4741 agccctgggt ggtttgcaaa tctaaaaact gtaccagcga caggtacgac aagtcaagtt
     4801 ggttttgtgc cgtcaccacc tgcaactcat cggcaacagc ctgtgcctcc atctgcaaca
     4861 cggttccctt ggctacggtg ccgacatcga acagggcttc ggtgcgtttc aactgctgcc
     4921 gggataactc aagctggttt tttgaagtct ccagcagttc caggttgtat aatatctgaa
     4981 ggtaggccgt agcaattgtc agcgaaatat cgtttttcat tttttcaagg tcatagctgc
     5041 tcgcaagaaa atcatattgt ttttgcttta atccgtttaa caaacgcagc ccgctaaaca
     5101 acaccacatt agaactcagg tagaaattat ttgattgaac ggtttgtgac gcaaaagtgt
     5161 tggtgtacat atccacggtt tggccattat tgtacacatg attagccgtg gcattcaccg
     5221 atgggaacaa ctcggctatg ctttggttgt actgaagctt cgacaaagcg gcattcagct
     5281 gttgctgctt cacctggata ttattttcca gcgcatgcga aatacaatcc tctaaagtcc
     5341 aaatcttttg tgaaaacaca tgggaacaac accacaaaaa gagcaaaaaa accgcggtaa
     5401 aaaacttttt catacaaatc ccaataagtt acaaagataa aaccttttta ggaagcattg
     5461 aaaaagcttg tgccaaatta tttgttatga agtcatgcca aaagaatatt gtgactattt
     5521 ttgtataata tcaaaaacct atgaaaaaac taattaccgg cctgctgttg ctcttttata
     5581 ttgccacggc ttcctatgcc gccgcgccgg caaaagcaat aagcgacact atcgacgtcc
     5641 ttaattactg tattaatgtt aatttgtcgc atatatccac taaaagcctg tcgggctctg
     5701 ccacccttga gtttataccg cggcacgaca atgtatctca ggtaaaactt tatttactga
     5761 aactgaatgt tgactccata ttttacgaaa acaacctcct gacaactttc tcctataacg
     5821 atactattat tgaaataaac ctgccccaac caatccatct gaacgatact gtttccctga
     5881 cggtgttcta tcacggcatg ccgcaaaccg atgcatcggg ctggggtgga ttttatttca
     5941 gcgccgacag
//
LOCUS       DAOSFP010000118         5908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11878_length_5908_cov_5.156330, whole genome shotgun sequence.
ACCESSION   DAOSFP010000118 DAOSFP010000000
VERSION     DAOSFP010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11878_length_5908_cov_5.156330"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2248
                     /locus_tag="PLI16_04700"
     CDS             <1..2248
                     /locus_tag="PLI16_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83890.1"
                     /translation="RYSLKVNPGKVTITYKYIGYTPVVRTYDLKPGQTKIEDIELYEE
                     SMVIEGIVVSAGKFEQKLSDVTISMAVLKPDRIEKQNTNNLSEVINKIPGIDVFDGQP
                     SVRAGSGYSYGAGSRVMVMVDDMPLLSPDAGDAKWNFMPIENISQVEVIKGASSALFG
                     SSALNGVINLRTAFPKDKPQTKITINEGVYLNPKRKELIWWDEYRPDYSNTLMAKIIN
                     PLSLLGMKNPGFGGVNFSHLQKFGQFDLGVGGNFFEDQGFRYPNGECRGRANVNLRYR
                     VKKIEGLSFGLNANVMYAATSNFFLWGNADSAAYMQREDAITHNQGLRANVDPYFMYF
                     NKRGSKYSMKLRWFRTSNLFPNEPDKNNISDIIFGDYQYQHNFKGKHNLTAGASGSYS
                     KSQADLFGEHDAVNFSIYAQYDAKFWKKLTLSLGIRGEYFRIDTAESESTFSYKIKGK
                     IHTLPIMPVFRAGLNYQAAKYTFIRASFGQGYRFPTVAEKFVETNLGGLNLFPNKQLK
                     PETGWAAELGIKQGIKIGKWNGYIDLAGFWTEYKNMMEFTFNDYRLDGRADTNYTGFE
                     EYINKFLGFKSLNVGQARIYGVDATFTGQGNLFGFPATILAGYTYTYPRNMDYDASDT
                     TGNSVKILKYRYLHSVKADLDVTIHFFTLGIGMNYTSHMINIDKAFEGELIPGIASSD
                     LLPGLKAYREEHNKGYFVMNLRGLFDITETQRVGVYVNNLLNTEYMTRPGFIEAPRNI
                     AVQYSLTF"
     gene            2254..3330
                     /locus_tag="PLI16_04705"
     CDS             2254..3330
                     /locus_tag="PLI16_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH83891.1"
                     /translation="MPEKKNILICPLNWGLGHASRCIPLIKILNQAGHHVVIAADGPA
                     ARLLKNVFPDNESVIFGDYNIRYSKGGHLVRTILWQIPKILFRIAAEHHKVKSIVKKY
                     NIDTLISDNRFGLWNRKIRTVYVTHQLFIKSPAGNRLVEKWLQKIHFFFIKKYDVCWI
                     PDLTGDFKLSGELSGKHPLPANARFIGLLSDFCDAPLQTTTGYDVCAVISGPEPQRSI
                     FQRLLLEQLKNSGKKAVVILGKPAEKSISTTDKNITVFNYLTADEIKKYFMCSEYVIA
                     RAGYSTIMDLAVTGRKAILVPTPGQTEQEYLAGYLKQKKIYYSTKQEGFDVNTALKKA
                     ANYSGISHCCDMQKLEEIILNTIS"
     gene            3374..4468
                     /locus_tag="PLI16_04710"
     CDS             3374..4468
                     /locus_tag="PLI16_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOH83892.1"
                     /translation="MLKRNKWLLIILGVLIIGFLIWYFWKIVVYILVAAVLSVMGKPL
                     VVLFDRIRIGKKKFPHALSALLTLFALYGVIFGFFALVVPMLISQITTLSSVGSDELL
                     LGLQEPINWLENFFVRFQIMKPGDSIEALVSAKLMSFISFANAGDLAGTLISFAGGFF
                     VAVFAIGFITFFFLKQERMLIKFIMLLTPVHLQTEIKHVYLKIIKLLSKYFLGLTLDL
                     VIVMTLITIGMTIFGYKNALMIGVFAGMMNVVPYVGPIIGCIIALVFASTGIIEAGGT
                     LETVPLILEVCGSLMVINAIDAYVFQPYIYSNVVKAHPLEIFLVILIAGSTAGILGMV
                     LAIPSYTVIRIIAKQFLIKYPVIKRLTQNI"
     gene            4534..5472
                     /locus_tag="PLI16_04715"
     CDS             4534..5472
                     /locus_tag="PLI16_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOH83893.1"
                     /translation="MNYLRLKGSYFLSILLQRPVHRGSPSFLSVEPGAFCNLRCPECF
                     TTQANFSRPRGFMTMDTFEKIMEQTAGSVFYLNLYFQGEPFLNDNIFDFIACAKKNGC
                     YTAVSTNGHFLDEANSNKLICSGLDRLIVSLDGTDAETYREYRRNGDFEKVVQGIKTV
                     VEQRKKTKSKHPFIEIQFVLTRKNQSQQHEIKKIGKQLGADKTTVKSFQLLNYERAGE
                     WLPDSNSRYLIADDGTVKTKNKLHNRCFRMWSSCVITWDGVVVPCCFDKNASYPLGNI
                     LHQPLNEIWDSETSREFRKKIFSQRKNIDICMNCCE"
     gene            5489..>5908
                     /locus_tag="PLI16_04720"
     CDS             5489..>5908
                     /locus_tag="PLI16_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOH83894.1"
                     /translation="MKKIAIFASGNGSNAENIINHFAGSQSVEIALILCNNSKAYVIE
                     RAKNHRIPCMIFKSKELRETPVVLNKLKELNIDFIVLAGFLLLIPTAIIRAFHNRIIN
                     IHPALLPKYGGKGMYGGFVHEAVRNSGDTETGISIHFV"
BASE COUNT         1745 a         1257 c         1264 g         1642 t
ORIGIN      
        1 ccggtactcg ctaaaggtca atcctggtaa agtaactatt acttataagt atatcgggta
       61 cacgccggtc gtgagaacct atgacctgaa acccggacaa acaaaaattg aagacattga
      121 gttgtatgag gaatcaatgg taatagaagg gattgttgtt tctgccggaa aatttgaaca
      181 gaaattgtcc gatgttacca tctcgatggc cgtattaaaa cccgaccgca ttgaaaaaca
      241 gaacaccaac aacctgagtg aggtaatcaa caaaatcccc ggaattgatg tgtttgacgg
      301 ccagcccagc gttcgtgccg gaagcggcta tagctacgga gccggcagcc gcgtaatggt
      361 aatggtggac gacatgccgc tgttatcgcc cgatgccggt gatgccaaat ggaactttat
      421 gcctattgaa aatatttcgc aggtagaagt gataaaagga gcctcctctg ccttgttcgg
      481 ctcttcagcc cttaacgggg ttatcaacct gaggaccgct tttcctaaag acaaacccca
      541 gaccaaaatc accatcaacg aaggcgtata tcttaaccca aaacgtaaag agcttatctg
      601 gtgggacgaa tacaggcccg attattccaa tacattaatg gctaaaatca ttaacccctt
      661 gtcgttactg ggcatgaaaa atcccggatt tggcggagtt aatttttcgc acttgcagaa
      721 attcggccag ttcgacctgg gcgtgggcgg caactttttc gaagatcagg gtttccgtta
      781 tcctaacggc gaatgccggg gccgtgccaa cgtaaacctg cgttaccgtg tcaaaaaaat
      841 tgaggggctt tcttttgggc tgaatgctaa tgttatgtat gcggccactt ctaacttttt
      901 cctgtgggga aatgccgatt cggcagccta tatgcagcgc gaagatgcga taactcataa
      961 tcagggctta cgagccaatg tggacccgta ttttatgtat ttcaacaaaa gaggcagcaa
     1021 gtatagcatg aaattaagat ggttccgcac atctaacctt tttcccaacg aacccgataa
     1081 aaacaatatt tccgacatta ttttcggcga ctaccaatac cagcataatt tcaaagggaa
     1141 acacaacctt actgccggcg catcgggcag ctactcaaaa tcgcaagctg atcttttcgg
     1201 agaacacgat gccgtcaact tttcaatcta tgcacaatat gatgccaaat tctggaaaaa
     1261 actcacatta tctttaggaa ttcgcggaga atacttccgc atagataccg ccgaatcgga
     1321 atccactttt tcatataaaa tcaaaggcaa aatacataca cttcctatca tgcctgtgtt
     1381 ccgtgccgga ttgaattatc aggcagcaaa atatacgttt atcagggcat catttggcca
     1441 gggataccgt tttccgactg tagcagaaaa atttgtggag accaacctgg gcggattaaa
     1501 cttatttccc aacaagcagc ttaaaccaga aacaggatgg gctgccgaat tgggaatcaa
     1561 acaaggtata aaaatcggaa aatggaatgg ctatattgat ttggccggat tctggactga
     1621 atataaaaac atgatggagt ttaccttcaa cgattaccgg cttgatggca gggccgatac
     1681 caattacacg ggttttgaag aatacatcaa taagttttta gggtttaaat cgttaaatgt
     1741 tggccaagca cgtatttatg gcgtggatgc tacatttaca gggcagggca atttgtttgg
     1801 ctttccggct actatcctgg caggatacac gtatacatat cccagaaaca tggattatga
     1861 tgcctcagat accacaggca acagcgtaaa aatacttaaa tacagatacc tgcattcggt
     1921 taaagccgac cttgatgtta ctatccattt ctttacgctc gggataggca tgaactatac
     1981 cagccacatg atcaatattg ataaggcctt tgaaggggaa ttaattccgg gcattgcttc
     2041 gtccgacttg cttccgggac ttaaagccta tcgcgaagaa cacaacaaag gttatttcgt
     2101 gatgaacctt cgcggattgt ttgatattac cgaaacccaa agagtgggtg tctatgtaaa
     2161 caacctgctc aataccgaat acatgacgcg cccgggtttc atcgaagcac ccagaaatat
     2221 tgccgtacaa tacagcctga ctttctgata atattgcctg aaaagaagaa tattcttatt
     2281 tgtccgctaa attggggact aggacatgcc agccggtgta tcccgctgat aaaaatttta
     2341 aatcaggccg gccaccatgt agttatcgca gctgacggac ctgctgccag gctgctaaaa
     2401 aatgtatttc ccgataatga aagtgttatt ttcggggatt acaatatcag gtattcaaaa
     2461 ggaggccacc tggtgagaac catcctttgg caaataccaa aaattttgtt ccgtattgca
     2521 gcggaacacc ataaggtaaa atcgattgta aaaaaataca acattgacac attgatttcc
     2581 gacaaccgtt ttggcctgtg gaaccgaaaa atccgtacag tgtatgttac tcaccagcta
     2641 ttcatcaaaa gccctgcggg aaatcgcctg gttgaaaagt ggctgcaaaa gattcacttc
     2701 ttttttatca aaaaatatga tgtgtgctgg atacctgatt tgacgggcga tttcaaactt
     2761 tcgggcgagc tgtccggaaa gcatccctta cccgccaatg cgcgttttat tggtctcctt
     2821 tccgattttt gcgatgcccc tctgcaaacc accacgggct atgatgtgtg tgccgtgata
     2881 tccggccccg agccgcaacg cagtattttt cagcgcctgc tgctggaaca attgaaaaat
     2941 tccggcaaga aagcagtggt cattttgggt aagcctgcag aaaaatcaat tagtacgaca
     3001 gacaaaaaca ttaccgtatt taattatctg acagccgacg agattaaaaa atattttatg
     3061 tgctcggaat atgttattgc cagggcggga tattccacca ttatggattt ggcggtaaca
     3121 ggccggaaag ctatacttgt gccaacgccc ggtcaaaccg aacaggaata cctggccggg
     3181 tatttaaaac agaaaaaaat ttattattcg accaaacagg aaggttttga tgtaaacaca
     3241 gcactaaaaa aagctgccaa ttattccgga ataagccatt gctgcgatat gcaaaaactg
     3301 gaagaaataa ttttgaatac aatttcataa ctattgtttt ctttttactt aattttgaca
     3361 caacgtattt gagatgctta agcgaaataa atggctgctg ataattctcg gagtccttat
     3421 cattggattt ctgatatggt atttttggaa gatagtcgtt tatattttgg ttgctgccgt
     3481 gctttcggta atgggtaaac cgctggtggt gctgttcgac cgtatccgca taggcaaaaa
     3541 aaaattccct catgcgctga gtgccttact tacattgttt gcactttatg gtgttatctt
     3601 tggttttttt gcgttggttg tacctatgct gatatcgcaa attaccaccc tgtcgtcggt
     3661 tggatccgat gaattattgc tcggactaca agagcctatt aattggcttg aaaacttttt
     3721 tgttcgcttt cagataatga aaccgggcga cagtattgag gcgctggtct ctgcaaaact
     3781 gatgtctttt atcagttttg ccaatgcagg cgatctggca gggacattga ttagttttgc
     3841 cggcggattt tttgtggctg tctttgccat aggatttata accttcttct ttctgaaaca
     3901 ggaacgtatg ctgataaaat tcatcatgct gctcacacct gtccacctcc aaaccgaaat
     3961 caaacacgta tacctgaaaa tcatcaaact gctttcgaaa tacttcctgg gactaaccct
     4021 cgacctggtc attgttatga cattgattac aatcggcatg acaatattcg gatataagaa
     4081 cgctttgatg ataggggttt ttgccggcat gatgaatgtt gtcccctatg taggccccat
     4141 tatcggctgt attatcgccc tggtatttgc ttccacagga atcattgaag caggcggaac
     4201 ccttgaaacg gtaccactga tacttgaagt atgcggatcg ttgatggtaa ttaatgccat
     4261 tgatgcgtat gtatttcaac cctatatcta ctccaacgtg gtaaaagccc atcctcttga
     4321 gattttcctg gtaatattga ttgccggcag tacagcagga atattgggaa tggtgctcgc
     4381 gatcccatca tataccgtga ttcgaatcat cgcaaaacag ttcttaataa aatatccggt
     4441 catcaaacgc ctgactcaaa acatctgacc gtgctgaatt catgcatgaa tgatttttat
     4501 catctgtcag ccgcgatgtc cggaaaaaaa ataatgaatt acctgcggct gaaaggctca
     4561 tattttttat cgatattgct tcaaagacct gttcacaggg gctccccttc ttttttatca
     4621 gtggagccag gggccttttg caacctgaga tgtcccgaat gttttactac ccaggctaac
     4681 ttttcgcgcc cccgaggttt tatgaccatg gatactttcg aaaaaattat ggaacaaact
     4741 gcaggaagcg ttttttatct caacctgtat tttcagggag agccgtttct taatgacaat
     4801 atttttgatt ttatcgcctg tgccaaaaaa aatggttgtt atactgcggt ttctactaat
     4861 gggcattttt tagacgaagc aaactcaaat aaactgatct gcagcggcct cgaccggctg
     4921 attgtttctt tagacggaac cgatgctgaa acatatcgcg aatacagaag aaacggagat
     4981 tttgagaaag tcgttcaggg gattaaaaca gtggtagaac aacgaaaaaa aacgaaatcc
     5041 aaacatcctt ttattgaaat acaatttgtg ctgacacgca aaaaccagtc acaacagcac
     5101 gaaattaaaa aaatagggaa acaactcggc gccgataaaa ccaccgtaaa atcttttcaa
     5161 ctgttgaatt atgaacgtgc cggcgaatgg ttgcccgata gcaactcccg gtatttaatt
     5221 gctgatgacg gcactgtgaa aacaaaaaac aagcttcata accgttgttt tcgtatgtgg
     5281 agttcctgtg tgattacatg ggatggagtg gtggttccct gttgtttcga caaaaatgcc
     5341 tcatatcccc tgggaaacat tcttcatcag ccgttgaacg aaatatggga cagtgaaact
     5401 tcccgtgagt tcagaaaaaa aatcttttcg caaaggaaaa acattgatat ttgcatgaat
     5461 tgttgcgaat gatttagtaa accaacaaat gaaaaaaata gccatttttg cctcgggcaa
     5521 cggaagcaac gccgaaaata tcatcaacca ctttgccggc agccaaagcg ttgaaattgc
     5581 tttgatactt tgcaacaaca gcaaagctta tgttattgaa cgcgcaaaaa atcaccgtat
     5641 tccctgtatg atttttaagt caaaggagct tagagaaaca cctgttgttt taaataaatt
     5701 aaaagaatta aatattgact tcatcgtgct ggcaggattt ttactgctga tccccaccgc
     5761 tattatccgg gctttccaca ataggatcat aaatatccat ccggctctgt tgccgaaata
     5821 tggcggcaaa gggatgtacg gcggttttgt tcatgaggct gtccgtaatt cgggcgatac
     5881 ggaaacgggt atttccatac atttcgtc
//
LOCUS       DAOSFP010000119         5890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11930_length_5890_cov_3.853642, whole genome shotgun sequence.
ACCESSION   DAOSFP010000119 DAOSFP010000000
VERSION     DAOSFP010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11930_length_5890_cov_3.853642"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..2557)
                     /locus_tag="PLI16_04725"
     CDS             complement(113..2557)
                     /locus_tag="PLI16_04725"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOH83895.1"
                     /translation="MIKRTLQSITAMLLIIMPGLTFGSEADLKIPELSASQNNILLFG
                     FLICFIGLSFGLYQFVKVKKLKAHQSMLDVSKVIFETCKTYLIQQGKFLILLFVFIAL
                     CIGFYFGFLQHTGPGGVLLIISWTVVGILGSYGVAWFGIRMNTLANSRMAFASLERKP
                     IKLLNIPLNAGMSIGVMLVCVELIMMLIILLFVPRELAGASFIGFAIGESLGASALRI
                     AGGIFTKIADIGSDLMKVVFKIGEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVT
                     GVALVSFIVLAVTNVDFQMELLTWIFVMRILMVITSIVSFYANRAISQSLYGKSDDID
                     FEKPLTNLVWITSIMSIAATFLASYFMIGPNSSIGATNGNLWLILSTIISFGTLGAAL
                     IPEFTKIFTSPKSKHVNEIVQASREGGASLTILSGLVAGNFSAFWKGMVFFGLMFGAY
                     YASTFGLVDLMIYPSIFAFGLVAFGMLGMGPVTIAVDSYGPVTDNAQSIYELSLIEEQ
                     KDIEKDIEQNFGFKPDFEKAKYYLEANDGAGNTFKATAKPVLIGTAVVGATTMIFSLI
                     LVIKNVVGIEPEQILNILNPYSILGLITGGAVIYWFTGASMQAVTTGAYRAVEYIKKH
                     INLDPNAPKIASVDKSKEVVKICTRYAQNGMFNIFIGIFAFALAFAFFSAPGDLDSTS
                     DMTQKLNLAAPVSFFVCYLISIAVFGLFQAIFMANGGGSWDNAKKVVEVDLKEKGTPL
                     HDATVIGDTVGDPFKDTSSVALNPIIKFTTLFGLLAMEIAISENFRQVAPIIGSIFFV
                     IALYFVYRTFYNMRIK"
     gene            2709..3320
                     /locus_tag="PLI16_04730"
     CDS             2709..3320
                     /locus_tag="PLI16_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOH83896.1"
                     /translation="MEKIRLDVVGITKSQTQSGAFAVILEDTIGKRRLPILIGYSEAQ
                     SIAIALENIKPPRPLTHDLFKSFCDSFNVKILEVIISKLSEGVFYSKLICFDGIRQVE
                     IDSRTSDAIALALRFDCRIFAYKPVMEAAHNIEGEEDEKEQEEKDTPRSDKKPDKELP
                     DESHDTEFEKYSLNELQDMMKEAIANEDYERASRIRDEINKRK"
     gene            3355..4611
                     /locus_tag="PLI16_04735"
     CDS             3355..4611
                     /locus_tag="PLI16_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOH83897.1"
                     /translation="MYLKQRFIAMNFLQFFIWGSWLISIGRFWFDYKEWSSSEFGIIF
                     STMGIASLFMPAIAGIIADRWVNAERLYGIFHLCGAIVLFILPNITSPTSFFWIMLLN
                     MIFYMPTIALAITVCYSSMKNAGLDIIKDYPPIRVFGTIGFIVALWIINLLRWGDTPN
                     QFYLASAASLLLGLYAFTMPKCPPLGKGHQTTFVSALGLNAFKLFKSYKMALFFIFSM
                     LLGAALQLTNAYGDTFIADFAKDPAYKDSWAVKYSEIIMSVSQISETLFILAIPFFLR
                     KLGIKKVMLMSMIAWVLRFGLFGFGTPEGWGLVLIILSCIIYGMAFDFFNISGSLYVE
                     TQSEPKIRASAQGLFMMMTNGVGAVAGSLLAGFIIQEYFTPDGVNKDWPGIWFTFAAY
                     ALVVAVLFAVLFKHKHDPKAMQSIKH"
     gene            4699..5703
                     /gene="gap"
                     /locus_tag="PLI16_04740"
     CDS             4699..5703
                     /gene="gap"
                     /locus_tag="PLI16_04740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOH83898.1"
                     /translation="MSVKIGINGFGRIGKLVFRLLQEKPEIEIVGINDPAEITVLTHL
                     LKYDSAHGRFTGTVEISGSHLIVNGKPIQVYHERIPANIPRTMHGADIVIEASGLFTT
                     REKLSQHLSSGARKVILTCPPQDVLDKTIIIGINQNTLAAADCLVSNASCTANCAAPL
                     LRILHENFGIEKVFMNTVHPATNNQRLIDAPHTDLRRSRTALNNIIPTTSSAIPVIKS
                     VMPFLNDCFDGLATRVPVDDGSLMELTAVVRKNTNIQEVNNAFLAASEDYLKGIVEYT
                     SDPIVSSDIIGNSHSAIFDSRATKVLSQNFIQIIGWYDNEYGYSSRVVDLIGIMKNLL
                     "
BASE COUNT         1672 a         1319 c         1264 g         1635 t
ORIGIN      
        1 gtttttattg tacttttaaa agattaaata tttttaaacg catcactact aatttaaaat
       61 gactttttat taacaatcat tttattaaaa ttaaaatagt tagctttttt tcctatttaa
      121 ttctcatgtt atagaaggtg cggtacacaa aatatagcgc aatcacaaaa aatatgctgc
      181 caataatcgg cgctacctgg cggaagtttt cagaaatggc tatctccata gccaataaac
      241 caaacagggt ggtaaatttt atgatagggt tgagggctac cgacgaggtg tctttaaagg
      301 gatcgcctac ggtgtcgcct atcactgtag catcgtgcag cggtgtgcct ttttctttca
      361 ggtccacttc cacaactttt ttggcgttat cccaggagcc gcctccattg gccataaaaa
      421 ttgcctggaa caacccaaat actgcaatgg aaatcaggta gcatacaaag aaagataccg
      481 gtgcagccaa attaagtttt tgagtcatat cagaagtgct gtccaggtct cctggtgcag
      541 aaaagaaagc aaatgccagg gcaaaagcaa atatgcctat gaagatgtta aacataccgt
      601 tttgagcata gcgagtacat atttttacca cctctttcga cttgtcaaca gaagcaattt
      661 tcggtgcatt ggggtcaagg ttgatgtgtt ttttgatgta ttccaccgca cggtaagctc
      721 cggtggtaac ggcctgcatc gaagcgccgg taaaccagta tatcacggcg cctcctgtaa
      781 ttaatccgag gatagaatac gggttcagaa tatttagtat ctgttcgggt tctatgccta
      841 ctacattttt gattaccagg atcagcgaaa aaatcatggt ggtggcaccc actacggcag
      901 ttccgatcag taccggcttg gcggtggctt taaaggtatt gcctgcgcca tcattggctt
      961 cgagataata cttggctttt tcaaagtctg gtttaaatcc gaagttttgt tcaatatcct
     1021 tttcgatatc tttttgctct tcaatcagcg agagctcata gatcgactgg gcattgtcgg
     1081 taacaggacc atagctgtcc accgcaatgg taacggggcc catgcccagc ataccgaagg
     1141 ccaccaggcc gaatgcaaag atcgaaggat aaatcatcag gtcaaccaat ccgaaggtgc
     1201 tggcataata agcgccgaac atcaggccga aaaataccat gcctttccag aaagcactga
     1261 aattgccggc taccagcccg gataaaatgg tcagggaagc gcctccttcg cgcgaagcct
     1321 gtacaatttc gttgacatgt tttgattttg gactggtaaa gatcttcgtg aattcgggta
     1381 tcagcgcggc acctaacgtt ccgaaactga tgatggtgga taatatcagc caaaggttgc
     1441 cgttggtggc gccaatagag ctgttggggc ctatcataaa ataactggca aggaaagtgg
     1501 cagcaatcga catgatggag gttatccaaa ccaggttggt aaggggcttt tcaaaatcta
     1561 tatcatcgct tttgccatat aatgactggc tgatagcccg attggcataa aacgaaacaa
     1621 tggaggtaat caccatgagg atacgcatta caaagatcca ggtcagcagc tccatctgaa
     1681 agtccacgtt ggtaacggcc agcacaataa atgataccag ggctacgccg gttactccat
     1741 aggtttcaaa cccgtcggcg gtggggccta cgctgtcacc ggcgttatca cccgtacaat
     1801 cggctattac acccggattg cgtggatcat cttcgccgat tttaaacact accttcatca
     1861 ggtcggagcc tatgtcggca atcttggtga aaatacctcc tgcaatacga agggccgaag
     1921 cgccaagaga ttcaccaatg gcaaagccga tgaaactggc accggcaagt tctctcggga
     1981 caaagagtag gatgatgagc atcataatca actccacgca aaccagcatg acacctatac
     2041 tcataccggc attcagcggg atgttcaaca gttttatggg tttacgctcc aacgaggcaa
     2101 aagccatgcg gctgttggcc agcgtattca tgcggatacc aaaccatgcc actccgtaag
     2161 atcccaggat gcctaccacg gtccaggaaa taataagtaa aacacccccg gggccggtat
     2221 gttgcaaaaa cccaaaataa aatccgatgc aaagggcaat aaaaacaaac agcaggatta
     2281 aaaattttcc ctgctgtatc agataagtct tacatgtttc aaaaataact ttggaaacat
     2341 caagcatgga ttgatgcgcc tttagttttt ttaccttgac aaattgatat aagccgaatg
     2401 acaaaccaat aaaacatatt aaaaaaccaa acagtaaaat gttattctgg ctggccgaca
     2461 attccggaat ttttaaatcg gcctcgctgc cgaaagttaa tccgggcata atgatcagca
     2521 acattgcagt aattgattgt aatgttcttt ttatcataat ttattattta aatttaggca
     2581 caaatataat aatttcacaa ttcaggacac ggtttttttt gtccttattt gtttcaaaac
     2641 aattttgcac attattcaga atggttatct ttgcatcata taatttgtcc actttataca
     2701 tcaattaaat ggagaaaatc aggcttgatg ttgttggaat aaccaaaagt caaacacagt
     2761 caggtgcttt tgcggtaatc ctcgaagata ctatcgggaa aagacggttg cctattctta
     2821 ttggttatag tgaagcccag tctattgcca tagctctcga aaatattaaa ccgccccgcc
     2881 cgctgacaca cgaccttttt aaatcgtttt gcgattcttt caacgtgaaa attcttgaag
     2941 taataatatc aaaactctct gaaggcgttt tttattcgaa actaatctgt tttgacggta
     3001 taagacaagt ggagatcgat tcccgcactt ccgacgccat cgctctggcc ttgcggtttg
     3061 attgccggat tttcgcatac aaaccggtga tggaagcagc tcataacatt gaaggtgaag
     3121 aagatgaaaa agagcaggaa gaaaaagata cgccgcgttc ggacaaaaag cccgacaaag
     3181 aattgccgga tgaatctcat gacacagaat ttgaaaaata ttccttaaac gaattgcagg
     3241 atatgatgaa agaagctatt gccaacgaag actatgagcg tgcttcgaga atccgcgatg
     3301 aaattaataa acgtaaataa ctctatactt ttttctaact aaaaccattt acttatgtat
     3361 ttgaaacagc gctttattgc tatgaacttt ttacagttct ttatttgggg ttcctggctg
     3421 atctctatcg gacgattttg gtttgattac aaagaatggt ccagctccga attcggaatt
     3481 attttctcca ctatgggcat tgcttctctt tttatgccgg ccatagcggg tattatcgcc
     3541 gacaggtggg tcaacgccga acggctctat ggcatctttc atctatgcgg agccattgtg
     3601 ctttttattt tgcctaacat aaccagtcca acatcctttt tctggatcat gctgctcaat
     3661 atgatttttt atatgcccac cattgcatta gcaatcacgg tttgctactc ttctatgaaa
     3721 aacgctggcc tcgatatcat taaagattat ccgccaatac gcgttttcgg aactataggt
     3781 tttatcgtgg ccttatggat tatcaatttg ctgcgctggg gcgatactcc caaccagttt
     3841 tatctggctt ctgcagcatc attgttgtta ggcctctatg cttttaccat gcccaaatgc
     3901 ccgccgctcg gcaaggggca tcaaaccacc tttgtgagcg cactgggatt aaatgcattc
     3961 aaacttttca aaagttataa aatggcgttg ttctttattt tctccatgct gttgggcgcc
     4021 gcactgcagc ttaccaatgc ctatggcgat acttttattg ccgactttgc caaagatcct
     4081 gcatacaaag attcgtgggc tgtgaaatat tccgaaatca ttatgtctgt ttcgcagata
     4141 tccgaaacgc tgtttatcct tgctatacca tttttcctga ggaagctcgg aataaaaaag
     4201 gtgatgctca tgagtatgat tgcctgggtg ctccggtttg ggttgttcgg atttggcacg
     4261 ccggaaggct ggggcctggt gctgattatt ctgtcgtgta ttatttacgg tatggccttt
     4321 gattttttca atatttcagg atcactttat gtggaaacac agagcgaacc taaaattcgt
     4381 gccagcgcac agggactttt tatgatgatg accaacgggg ttggtgcggt ggctggcagc
     4441 ttattggcag gatttattat tcaggagtat tttactcccg acggtgtgaa caaagactgg
     4501 ccggggatat ggtttacttt tgcagcctac gcacttgttg ttgccgtatt gtttgccgtg
     4561 ttgtttaaac acaaacacga ccccaaagcg atgcagtcca taaaacactg acaccaatca
     4621 gcattaccaa tggatgaaat tgcaatgaaa tgaactccgt tattacggac acctgttttt
     4681 tattaatatc ataaaagaat gtcagtaaaa atcggaataa acgggtttgg caggataggc
     4741 aaactggttt tcagactgct ccaggagaag ccggagattg aaattgtggg tattaacgac
     4801 cccgccgaaa ttaccgtgct tacccacctg ttgaaatacg attcggcaca tggccgtttt
     4861 accggaacag tggagatttc gggcagccac cttattgtca acggtaagcc catacaggtc
     4921 tatcacgaac gaatacctgc caacataccc cgcactatgc acggggccga tatcgtaatc
     4981 gaagcctccg gattgtttac cacacgcgaa aaactttcgc agcacctgag ctccggagca
     5041 agaaaagtga ttcttacctg tcctccccag gatgtgctcg acaaaaccat aattatcggt
     5101 ataaaccaaa acactctcgc cgccgccgac tgtctggtgt ccaatgcttc ctgtacggct
     5161 aactgcgccg cacccttgct gcgtattctt cacgaaaact ttggtatcga aaaagttttt
     5221 atgaatactg tgcacccggc tacaaacaac cagcggctta ttgacgcacc ccataccgac
     5281 ctccggcggt cacgcacagc tctcaataat atcattccca ccacctccag cgctattccc
     5341 gtgataaaaa gtgttatgcc tttccttaac gattgtttcg atggcctggc cacgcgggtt
     5401 cctgtggacg atggctcatt gatggaatta acggctgtgg tcaggaaaaa tactaatatc
     5461 caggaggtaa acaatgcttt tctagcggcc tctgaagatt acttgaaagg aattgtggaa
     5521 tacaccagcg atcccattgt ttcaagcgat atcattggta attcacattc agcaatattc
     5581 gacagccgcg ccaccaaagt gctcagtcaa aactttatcc agatcattgg ttggtatgat
     5641 aatgaatacg gttattcgag ccgggtggtg gacctgattg gtattatgaa aaatctcctg
     5701 tagaacattt aagtctatat ccattgacga ccaacaacta caacgaaaca ctgctgatcc
     5761 gcggatttct tgattccgcc ggcaaattcg gtagccggcc agccctggat attgataaca
     5821 gccagactac ctatgacgaa ttgttgctga ttgccggaaa gatagccaca ctcatcgctg
     5881 ccaatacgca
//
LOCUS       DAOSFP010000120         5884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_11952_length_5884_cov_4.438840, whole genome shotgun sequence.
ACCESSION   DAOSFP010000120 DAOSFP010000000
VERSION     DAOSFP010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11952_length_5884_cov_4.438840"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLI16_04745"
     CDS             complement(<1..717)
                     /locus_tag="PLI16_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018572241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOH83899.1"
                     /translation="MTTYEKTVAYMFDCLPMFHRIGAAAYKANLDNTLALSKLCGKPE
                     LDFPSIHIAGTNGKGSVSHMIASIMQEQGLKTGLYTSPHLKDFRERIRVNGKMIPKHK
                     VVQFVNKHKKDFEAILPSFFEITFAMAMEYFSEQKVDIAIVETGMGGRLDSTNIVSPV
                     ITVITNIGYDHTQFLGDTLPKIAAEKAGIIKPGIPVIIGITQAETKTVFTETASKNNS
                     KIIFADTLLHLKNFRFVQEPP"
     gene            complement(1407..2138)
                     /locus_tag="PLI16_04750"
     CDS             complement(1407..2138)
                     /locus_tag="PLI16_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOH83900.1"
                     /translation="MKLDKYQKGGIAGTLLFHALLLVCFIFFGFTTPLPLPEEEGVEV
                     NLGYSDDGMGDIEPTDPFSEKSVSYTNPNNADENITQNTEESATLNNKNNNKNTTPQE
                     EIRIDKNIAYNKNKNSDGGSQGITGKPGNQGNPNGNPNSDNYNGNSPYGDGISFKLSD
                     RKSKSLPKPNYNSKDEGSVVVNIWVDKQGNVTKAQAGARGTTTTSTALWKLAEDAAKR
                     SKFDTNPSAPEEQKGTITYNFVNLN"
     gene            complement(2170..2583)
                     /locus_tag="PLI16_04755"
     CDS             complement(2170..2583)
                     /locus_tag="PLI16_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOH83901.1"
                     /translation="MAIQMRNKINPEFSLSSMSDLVFLLLIFFMLTSTLVSPNAIKLL
                     LPNSNSKTIAKQTTTVYINDQYQYFIEDRPMAEAALVPAIEAKLGGQNDATIVLRTDK
                     SVPVQYVVNVIDAVNQINDKNNSKHKVILATQPKD"
     gene            complement(2597..3328)
                     /locus_tag="PLI16_04760"
     CDS             complement(2597..3328)
                     /locus_tag="PLI16_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOH83902.1"
                     /translation="MLGIILQITQSGTIADTAAAVAQQAQQLPQVAAPTSETLSIWEL
                     TMKGGWIMIPIALLSVLAIYIFIERFMATQKAAKEDPGFMNNIRDFIHQGKLDAALSL
                     SKNTDTPIARMLHKGLLRLGKPMSDINVTIENVGKQEIAKLEKRITLLAMAAGGAPML
                     GFLGTVTGMVRAFYNMSKAGNNIEIATLSGGIYEAMITTVGGLIVGIIAYFCYNIIVA
                     RIQKIVNMLELRASEFMDLLNEPAN"
     gene            complement(3442..4425)
                     /locus_tag="PLI16_04765"
     CDS             complement(3442..4425)
                     /locus_tag="PLI16_04765"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83903.1"
                     /translation="MKKHLLVILLIFTVLLATGQQKHVVTYPDGKVHYEYETKNGLLD
                     GPFVSYYENGNKKAEGKYVLNQRKGQWKVWDSRGLRRAERVYGNSFDYRITGLWDSTG
                     NKCKVRQKATKKYSTVDPAFGYVPYYPVTEKEVLWCKRLWREIPADSLINQQLFLNNR
                     LFGLIMEAIMTNKISAFRDEEYEHALPYDSVQEYKNLEVACYRIKEDFFFNKTLSLTE
                     YRTIGIGPAMKKSGKTKTLFWLYYPEIRGLLASQQPETETPNTMIPNYESLFFKRYYH
                     SIIYKESNVYDREIKDYKKGKDIGKEAEIIELTAIDLEFEWWYNNTCPCKK"
     gene            complement(4432..5739)
                     /locus_tag="PLI16_04770"
     CDS             complement(4432..5739)
                     /locus_tag="PLI16_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008765136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOH83904.1"
                     /translation="MRHLLIVLTIILSLPFGLTGETQAQPSGTDLYNKLKSLPGVQEV
                     QALKHSTPFKEKYLVLLKQWLDPMDTAAGTFVQRVFVSHYAFDAPTVLVTEGYAGTYA
                     EREDYTEELTGLFKTNQILVEHRYFGKSTPAIKDWKYLTATNAAADHHRVVSLFKNVY
                     KNKWINTGISKGGQTALIHRALYPGDVDISVCYVGPLCFNVEDGRHEPFISSKVGTAS
                     DRQKIQDFQMEVLKRRNKIFPLFKKFCNEKKYVFHLPLQEIYDYCVLEFSFSFWQWGW
                     KPSSIPSTSASDSDLFDYLIRVCGPDYFADDGQDYSQSFFVQAARELGYYGYDTEPFK
                     KYLKIKTAKGYLGKIFLPEGLKIDFDSTLSLKCQHFLDTQDEKMIFIYGGYDPWSAPA
                     VEFSGKKNMFKAVCPGGSHATRIRSFGEKEKQEIIDRIKTWLE"
BASE COUNT         1451 a         1185 c         1336 g         1912 t
ORIGIN      
        1 tggaggctcc tgcacaaaac gaaaattttt caggtgcagg agtgtatcgg caaaaataat
       61 tttgctgtta ttttttgaag cagtttcggt aaataccgtt ttggtttctg cctgcgttat
      121 tccaatgatt acaggtattc cgggctttat gatgccggct ttttcggcag caatttttgg
      181 gagcgtatcg cccaaaaact gggtgtggtc ataaccaatg ttggtaatta cggtaatcac
      241 cggactaaca atattggtgg aatcgagacg gcctcccatg ccggtctcca caatggcaat
      301 atcgactttt tgttccgaaa aatattccat agccatggcg aaagtgatct caaaaaatga
      361 aggtagtatg gcctcaaaat cttttttgtg tttgtttaca aactgtacta ccttatgttt
      421 gggtatcatt tttccgttga cacggatgcg ttcgcgaaag tctttcagat gcggggaggt
      481 gtacaggcct gtttttaatc cctgttcctg catgatggaa gcaatcatgt gcgaaacgga
      541 ccctttgccg ttggtgcctg caatatgaat ggagggaaaa tccaattccg gttttccgca
      601 cagtttgctg agtgcaaggg tattgtcgag gttggctttg tatgcagcgg ctcctatacg
      661 gtggaacatc ggcaggcagt cgaacatgta ggcaacggtt ttctcgtaag tggtcattta
      721 tttgtaattc aggaatgcaa aattacatca atctgcccat taccgttcaa aattatgtac
      781 taaattaaac acggatttgt cactagaaac gatgatcgct aatgacaagt gtgttagctg
      841 gaccgttgta tttcatcaac cttaataaaa ctaactatat ttcactaaat tcaaaattta
      901 atgaatgatc tgtagttatt caaacatttt attttttcgt agaaaaaaag tgcgttggcc
      961 tgcccagacg gcgggccagc tcaggcgtga atcagaaaat atttgaaaat cttcaaaggc
     1021 cccgcagatt gcggggtttc ccgacgcatg gcgtcgggcc tactttgatt agattttcaa
     1081 tattttctga gagcgggctg aagcattgtc tgggctgtct ttttctttgg ttcatttctt
     1141 ttgcacaagc aaaagaaatg aacgtccaaa tcaatacaca atcattttaa aagttagatg
     1201 ttttattcta ctttttcaaa gtcttgaaat ccgggataac cctggcttag tgtgtgttag
     1261 attttctttc aaggtttata tatcgcaaat cctataaaca cttagtcgct gtatagcaga
     1321 aaatcgtata atgacgattt taggggaacc cattttagac acgatagtac ttaaagcact
     1381 tcgtgcacta cactaagtaa tcctttttaa ttcaggttta cgaaattata ggtaatggta
     1441 cctttttgtt cttccggagc ggaagggtta gtgtcgaatt tcgaacgttt ggcggcatct
     1501 tcggcaagtt tccacagggc ggtactggta gtggtggtac ccctggcgcc tgcctgtgct
     1561 ttggttacat tgccctgttt atccacccaa atgtttacta ctaccgaccc ttcatctttc
     1621 gagttgtagt ttggtttggg caaagatttg gatttgcggt cggaaagttt aaaagaaata
     1681 ccgtcaccgt atggactgtt gccattgtag ttgtcagagt tcggattgcc gttgggattc
     1741 ccctggttgc ccggctttcc tgtgattccc tgcgaccctc cgtcggaatt tttattcttg
     1801 ttgtaagcta tgtttttatc aatccttatt tcttcctgag gggtggtatt tttgttgttg
     1861 tttttattgt tcagggtagc gctttcttcg gtgttttggg tgatattttc gtcggcgtta
     1921 tttgggttgg tgtagcttac agatttttcc gagaagggat ctgttggttc tatgtctccc
     1981 attccatcat cgctgtatcc gaggttaact tccacacctt cttcttcggg caggggcagg
     2041 ggcgtggtaa agccaaaaaa tataaaacac accaaaagca gggcgtgaaa caatagggtg
     2101 cccgcaattc ctcctttttg atatttatct aatttcatgg ctgttcgtat ttatttttta
     2161 tacgaaaaat taatcttttg gttgtgtggc caggattact ttgtgttttg aattgttttt
     2221 gtcgttaatc tggttcactg catcgatgac attgaccaca tattgcacgg gaacactctt
     2281 gtcggttctc agcacaatgg tggcgtcgtt ttggcctccc agtttggctt caatggcggg
     2341 aaccagtgct gcttcggcca tgggcctgtc ttcaatgaaa tattgatact ggtcgttgat
     2401 atataccgtg gtggtctgtt tggcgatggt tttgctgttg ctgttgggta aaagtaattt
     2461 tatggcattt gggcttacaa gtgtggaagt aagcatgaaa aatatcagca gcaggaatac
     2521 caggtcagac atggatgaca agctaaattc cggattaatt ttgttgcgca tttgtattgc
     2581 catagtcgga atgtttttaa ttggcaggtt cgttaagcag atccataaat tcggaagctc
     2641 ttaattcgag catattcaca attttctgaa ttcgggcgac gatgatgtta taacagaaat
     2701 aggcgataat tccaacgata agaccaccca ccgtggttat catggcttcg taaatacctc
     2761 ctgacagggt ggcaatttca atgttgttgc cggccttgga catattatag aaggctctca
     2821 ccataccggt tacggttccc agaaatccca gcataggggc gcctccggca gccatggcaa
     2881 gcagcgtaat gcgtttttca agttttgcaa tttcctgttt tcctacattt tctatggtca
     2941 cgttaatatc gctcatcggt ttgcctaaac gcagcaatcc tttatggagc atccgggcaa
     3001 tcggtgtatc ggtgtttttg gaaagggata gtgctgcatc aagtttgccc tgatggataa
     3061 aatccctgat attattcata aacccgggat cttctttggc ggctttttgt gtggccataa
     3121 atctttcgat aaaaatataa atggctaata ccgacagtaa agcaataggg atcatgatcc
     3181 atccgccttt cattgttaat tcccatatcg aaagggtttc tgatgtggga gcagcaacct
     3241 gcggcagttg ttgtgcctgt tgtgctacag ctgccgctgt atccgcaatg gttcccgatt
     3301 gggtgatttg caaaattatt ccaagcataa aaaaatattt ataaatatgt aaaggtagcg
     3361 gaattgccta tgatggtagt ggtaaagggt gtttattttt taacacgcgg ttgttaatat
     3421 tacaacgctg caagtggcga tttatttttt acaggggcaa gtattattgt accaccattc
     3481 aaattcaagg tcaatggctg tcagctcgat gatttcggct tcttttccta tgtctttgcc
     3541 ttttttataa tccttgattt ctctgtcata aacattgctt tccttataga taatgctgtg
     3601 atagtatctt ttgaaaaaca ggctctcata gttgggtatc atggtattgg gtgtctctgt
     3661 ctcaggttgt tgcgatgcca gcaagccgcg tatttcaggg taataaagcc agaaaagtgt
     3721 ctttgttttg cctgattttt tcatggccgg tcctattcca atggtacggt attccgttag
     3781 gctcagggtc ttgttgaaaa aaaaatcttc ttttatcctg tagcaggcta cctccaggtt
     3841 cttatattcc tgcaccgaat catacgggag cgcatgctca tattcttcat cacggaaggc
     3901 cgaaatctta ttcgtcatga ttgcttccat gatcaggccg aacagcctgt tgttcaggaa
     3961 tagttgctgg tttatcagac tgtcggcagg tatttcgcgc cacagccttt tgcaccaaag
     4021 cacttctttt tcggtaacgg ggtaataagg tacgtagcca aatgcaggat cgactgtaga
     4081 atactttttg gtggccttct gtcttacttt gcatttattt ccggtgctgt cccaaaggcc
     4141 tgtgattcgg tagtcaaaag aatttccata aaccctctct gccctcctga ggccacggga
     4201 gtcccatact ttccattgtc ccttgcgctg gtttaatacg tattttcctt cggccttttt
     4261 gttgccgttc tcataataac tgacaaaggg tccgtcgagc aatccgtttt tggtttcgta
     4321 ttcataatgt accttgccat caggatatgt gacgacgtgt ttttgctgtc ccgtggccag
     4381 caagaccgtg aaaatcagta aaataacaag caggtgtttt ttcatgattg attattcgag
     4441 ccaggttttt atgcggtcga tgatttcctg tttttctttt tcgccaaaag aacgtatgcg
     4501 ggtggcatga cttcctccgg gacacaccgc cttgaacatg tttttctttc cgctgaattc
     4561 cacagccggt gccgaccagg gatcgtatcc gccatagatg aaaatcattt tttcatcctg
     4621 ggtgtccaga aaatgctgac acttgagaga aagggtgctg tcgaaatcaa tttttaaacc
     4681 ttccggcaaa aatattttcc cgagataacc ttttgccgtt tttattttca ggtatttttt
     4741 aaaaggttcg gtgtcataac cgtagtaacc cagttcccgg gcagcctgaa caaaaaaaga
     4801 ttgcgaatag tcctgaccat catcggcaaa atagtcggga ccgcacaccc ggatgaggta
     4861 atcaaacaga tcgctgtcag aggcgcttgt tgaaggaatg gaggatggct tccaacccca
     4921 ttgccaaaac gaaaacgaaa attccagcac acaatagtcg tatatttcct gtaatggcag
     4981 atggaagacg tatttttttt cattgcagaa ttttttgaac agggggaaaa ttttgttgcg
     5041 tcttttcagt acttccatct ggaagtcctg gatcttttgc cggtcacttg ctgtgccaac
     5101 tttgctgctg atgaatggtt cgtgcctgcc gtcttccaca ttaaaacaca atggcccgac
     5161 ataacatacc gaaatatcca catcaccggg atacaatgca cggtgtatca gggccgtttg
     5221 ccctcctttg ctgatgccgg tgtttatcca tttattttta taaacgtttt tgaagaggga
     5281 taccacacgg tggtggtctg ctgcggcatt ggttgccgtg aggtatttcc agtctttaat
     5341 agccggcgta gatttcccaa aataacggtg ttcgaccaga atttggttcg tcttgaaaag
     5401 accggtaagt tcttcggtgt aatcttcgcg ttcggcatag gtgcctgcgt atccttcagt
     5461 taccagcact gtaggggcat caaaagcata atgcgaaaca aaaacccgtt gcacaaaagt
     5521 gccggcggct gtatccatgg ggtcgagcca ttgttttaac aatacaagat acttttcttt
     5581 aaacggtgtt gagtgtttta atgcctgaac ttcctgcacg cccggcagtg atttcagttt
     5641 attatataaa tcagttccgc tgggttgggc ttgtgtttct cctgtcaggc caaaaggaag
     5701 ggacaaaatg attgtcaaca caatcaacag gtgtctcata gctttttgtg tcattggtat
     5761 ggtttatttg ggtttaattt gtcgagtaag aaccaaaatt cgaatgtatt agtaagagtt
     5821 gattgctatt atcaacttaa atcacgaatg cgtttcaggt caaatttagg gttttaagtt
     5881 gata
//
LOCUS       DAOSFP010000121         5860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12017_length_5860_cov_4.274591, whole genome shotgun sequence.
ACCESSION   DAOSFP010000121 DAOSFP010000000
VERSION     DAOSFP010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_5860_cov_4.274591"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="PLI16_04775"
     CDS             complement(<1..863)
                     /locus_tag="PLI16_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOH83905.1"
                     /translation="MKRLIETIKNIYRIEDLRKRIIYTLGIILIYRVGSYVVLPGIDP
                     NMLGALQRQTDDGLLGLLNMFSGGAFANASIFALGIMPYISASIVIQLLGMAIPYFQK
                     LQKEGESGRKKINQITRFLTVIITAAQAPAYIGNITSQLPSEAIYPFMPETAPTVFFW
                     VSSVIILVSGTMFVMWLGERITDKGIGNGISLIIMIGIIARLPFALVGEFISRMEQQG
                     GGLIIFVVELVVLVLVILVCILLVQGTRRIPVQYAKRIVGNKQYGGVRQYIPLKVNAA
                     GVMPIIFAQAI"
     gene            complement(867..1313)
                     /gene="rplO"
                     /locus_tag="PLI16_04780"
     CDS             complement(867..1313)
                     /gene="rplO"
                     /locus_tag="PLI16_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOH83906.1"
                     /translation="MDLSNLKPAKGSIKSSKRIGRGQGSGKGGTSTRGNKGAQARSGY
                     SKKLAFEGGQMPLIRRIPKFGFNNISRVEYSGINVDTLQKLAEEKNLTAIDVNTLIEN
                     GLASKNELIKILGRGELKLKLDVKAHAFSATAVKIIEAQGGTATKI"
     gene            complement(1339..1518)
                     /gene="rpmD"
                     /locus_tag="PLI16_04785"
     CDS             complement(1339..1518)
                     /gene="rpmD"
                     /locus_tag="PLI16_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOH83907.1"
                     /translation="MKKIRVTQVKSGIDRPAIQKKTLLALGIKKMHQSVELEATPQVE
                     GMVRKVKHLVTREEI"
     gene            complement(1543..2061)
                     /gene="rpsE"
                     /locus_tag="PLI16_04790"
     CDS             complement(1543..2061)
                     /gene="rpsE"
                     /locus_tag="PLI16_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOH83908.1"
                     /translation="MSNANIKRVKSSEIEFKDRLVSIQRVTKVTKGGRTFSFSAIVVV
                     GNENGVVGYGLGKAKEVTAAIAKGIDDAKKNLVKVPVYNGTVPHEQYGKYSGAYVFLK
                     PATPGTGVIAGGAMRAVLESVGVKNVLAKSKGSSNPHSVVKATMDALVNMKDPYTVAQ
                     LRGVTLGQVFNG"
     gene            complement(2068..2430)
                     /gene="rplR"
                     /locus_tag="PLI16_04795"
     CDS             complement(2068..2430)
                     /gene="rplR"
                     /locus_tag="PLI16_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOH83909.1"
                     /translation="MAIKSNKEYRRFRIKQRIRKIVEGTVERPRMSVFRSNTNIYVQL
                     IDDVNGKTLVAASSLDPEIAAAKVDKKEKAKMVGKVIAQKAIEAGLNAVVFDRGGYLY
                     HGRVKSLADSAREAGLKF"
     gene            complement(2457..3008)
                     /gene="rplF"
                     /locus_tag="PLI16_04800"
     CDS             complement(2457..3008)
                     /gene="rplF"
                     /locus_tag="PLI16_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOH83910.1"
                     /translation="MSRIGKLPIALPQNVQLNVSDDNIVTVKGPKGELKQKINPSVKI
                     KVENNHIILERENDEARAYHGLYRALVSNMVHGVSEGFKLVQEIIGVGYKADAQNNLL
                     ELSLGYSHNIFIEMPPDVKVTTINERGKPPTIILESIDKQLLGQVASKIRSLRKPEPY
                     KGKGIRFQNEVLRKKAGKSAVQK"
     gene            complement(3026..3424)
                     /gene="rpsH"
                     /locus_tag="PLI16_04805"
     CDS             complement(3026..3424)
                     /gene="rpsH"
                     /locus_tag="PLI16_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOH83911.1"
                     /translation="MNTDPISDYLTRIRNAVMANHRMVEIPASNLKKSITKILFEKGY
                     ILNYKFEDNPKPGTIKIALKYHPTMKTSAISTLERVSSPGLRKYVNADELPRVLNGLG
                     IAIISTSKGIMTDKEARQQNVGGEVICYVS"
     gene            complement(3461..3730)
                     /gene="rpsN"
                     /locus_tag="PLI16_04810"
     CDS             complement(3461..3730)
                     /gene="rpsN"
                     /locus_tag="PLI16_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOH83912.1"
                     /translation="MAKESIKARELKREQLVEKYKEKRLQLKKEGDYIALQKLPKNAS
                     PVRLHNRCKITGRPKGYMRQFGISRINFREMALAGLIPGVKKASW"
     gene            complement(3732..4286)
                     /gene="rplE"
                     /locus_tag="PLI16_04815"
     CDS             complement(3732..4286)
                     /gene="rplE"
                     /locus_tag="PLI16_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOH83913.1"
                     /translation="MDYKPRLKEKYDKEIVPALIKQFQYKNNMQVPRLLKISLNQGVG
                     GAIADKKMIETGIDEMSLVSGQKAVQTKSKKDISNFKLRKGMPIGVRVTLRNEKMYEF
                     LDRLISVAIPRIRDFRGINDKGFDGKGNFSFGITEQIIFPEITIDKVSKINGMNITMV
                     TSAKNDQEAYALLKEFGLPFKNQK"
     gene            complement(4286..4615)
                     /gene="rplX"
                     /locus_tag="PLI16_04820"
     CDS             complement(4286..4615)
                     /gene="rplX"
                     /locus_tag="PLI16_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOH83914.1"
                     /translation="MRPKIHVKKGDTVTVIAGDSKGQQGKVLKVIVEKNKVLVEGVNL
                     ISKHTKPNAKNTKGGILKKEASIHVSNLMVNDSSGKPTRTGRKIDEKSGKLVRYSKKS
                     EKKETIK"
     gene            complement(4627..4995)
                     /gene="rplN"
                     /locus_tag="PLI16_04825"
     CDS             complement(4627..4995)
                     /gene="rplN"
                     /locus_tag="PLI16_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOH83915.1"
                     /translation="MIQQESRLIVADNSGAKEVLCIRVLGGTKRRYASVGDKIIVTVK
                     SAIPAGNIKKGTVSKAVIVRTSKEVRRNDGSYIRFDDNAVVLLTANDELRGTRIFGPV
                     ARELREKQYMKIISLAPEVL"
     gene            complement(4997..5251)
                     /gene="rpsQ"
                     /locus_tag="PLI16_04830"
     CDS             complement(4997..5251)
                     /gene="rpsQ"
                     /locus_tag="PLI16_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOH83916.1"
                     /translation="MERNLRKEKIGVVVSNKMQNTIVVNVERKVMHPKYGKFVKKNTK
                     FMAEDAKNECSIGDTVKIAETRPLSKNKCWRLVEIIEKVK"
     gene            complement(5261..5500)
                     /gene="rpmC"
                     /locus_tag="PLI16_04835"
     CDS             complement(5261..5500)
                     /gene="rpmC"
                     /locus_tag="PLI16_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOH83917.1"
                     /translation="MKQQVISELSTNELRERLEEEKKQYLKLKMNHAISPLENPMKIK
                     DYKRTIARMLTELRKRDLAGDTQQVEVVTKKNSKK"
     gene            complement(5507..>5860)
                     /gene="rplP"
                     /locus_tag="PLI16_04840"
     CDS             complement(5507..>5860)
                     /gene="rplP"
                     /locus_tag="PLI16_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOH83918.1"
                     /translation="QISFGTFAIKTLDEYWITGRQIEAARQAITRHMKREGQLWIRIF
                     PDKPVTKKPAEVRMGKGKGALEYYVARVTPGRIMFEIDGVPFDIAKKALELGAQKLPV
                     HTRFVIRKDYIEDQK"
BASE COUNT         1382 a         1231 c         1129 g         2118 t
ORIGIN      
        1 ataatggcct gtgcaaaaat gataggcata acaccggcag cattcacttt taatggaata
       61 tactgacgta cgccgccata ctgtttgttt cctacaattc tcttggcata ttgtacggga
      121 attcttctgg ttccctgaac aagcaggata cacactaaaa taaccagaac caaaacgacc
      181 aattccacaa caaaaattat gaggccacca ccctgctgtt ccatgcggga aataaactcg
      241 ccgactaaag cgaaaggcag acgggcgata ataccgatca taatgattaa tgaaataccg
      301 ttgccgatac ccttatcggt gatgcgttcg ccgagccaca taacaaacat ggttcctgaa
      361 acaagaatga ttaccgatga cacccagaaa aacactgtag gagccgtttc gggcataaaa
      421 ggataaatgg cttcgctggg cagttggctg gtgatgttgc cgatataggc gggtgcctga
      481 gctgctgtaa tgattactgt taagaaacgt gtgatttggt tgattttttt cctgcctgat
      541 tcgccttctt tttgcagttt ctggaaatag ggaattgcca taccaagcaa ctgaataacg
      601 atagaggcag agatatacgg cataatacca agggcaaaaa tggatgcgtt ggcaaatgca
      661 cctccggaga acatattaag aagtccgagc aggccatcat ccgtttgcct ctgcaatgcg
      721 cccagcatat tggggtcaat gcccggcaat accacataag aacccacgcg gtaaatcaga
      781 ataattccca gagtatagat aatcctcttg cgaagatctt ctatcctgta aatattttta
      841 atagtttcta tcagtcgttt cattcattaa atttttgttg cggttccacc ttgtgcttca
      901 ataattttta cggcagtagc tgaaaatgca tgagctttca catcaagttt taatttcagt
      961 tcgcctctgc ctaaaatttt tatcagttcg tttttggatg caagtccgtt ttctatcaat
     1021 gtgttaacat caatagctgt aaggtttttt tcttcggcaa gtttctgaag tgtgtccaca
     1081 tttattccac tgtattccac cctgctgata ttattaaatc caaattttgg aattcttctg
     1141 atcaaaggca tctgaccgcc ttcaaaagct aattttttag aataaccgga tcgtgcctga
     1201 gcgcctttat tacctcttgt ggaagttcct cctttaccag agccttgtcc tcttccgata
     1261 cgttttgaac tttttatcga tccttttgcc ggtttcagat tacttaaatc catagtatat
     1321 taattatttt tttcttaact aaatttcttc tcttgttact aaatgtttga cttttctgac
     1381 cataccctcc acttgtgggg tggcttcgag ttcaacggac tggtgcattt tctttattcc
     1441 taatgccagc aaggtttttt tctgtatagc cggtctgtct attccgcttt ttacttgtgt
     1501 taccctgatt tttttcatat tcattgtatt tatagtagcc tattaaccat taaaaacctg
     1561 acctaatgta acacctctca gttgtgccac tgtataaggg tctttcatgt ttaccaatgc
     1621 atccatggtt gctttcacca cgctgtgcgg atttgaggat cctttggatt ttgccagcac
     1681 gtttttcacg ccaacgcttt ccagtacggc acgcatagct ccaccggcaa taacaccggt
     1741 accaggggta gcaggtttga gaaacacgta tgcaccgcta tattttccat attgttcgtg
     1801 aggcacagtg ccattataga cgggtacctt gacgaggttt ttcttggcat cgtcaatacc
     1861 tttggcgata gctgcggtaa cttcttttgc ttttcccagt ccataaccta ccacgccgtt
     1921 ttcgtttcct acgacaacga tagcagaaaa actaaaggtc cttcctcctt tggtaacttt
     1981 ggttactctc tggatgctta ccaacctgtc tttgaactct atttcgctcg acttaactct
     2041 tttgatgttt gcgtttgaca tatattatta aaattttaaa cctgcttctc ttgccgaatc
     2101 agctaatgat tttactctac catgatataa gtatccgcct ctgtcgaata caacagcgtt
     2161 aagaccggct tctatagctt tttgtgctat tactttgcca accatttttg ctttttcctt
     2221 tttgtcaact tttgctgcag caatttcggg atcgagtgat gaagctgcta ccagagtttt
     2281 tccgttcacg tcgtcaatca gttgaacgta aatattggta ttacttctga agactgacat
     2341 tctgggacgt tcaaccgtac cttccacgat ttttctgatc cgctgtttta ttctgaatct
     2401 tctgtactct ttattacttt taatagccat tactctgtga gttttaacgt tttacattat
     2461 ttttggacgg ccgattttcc ggctttcttt ctaaggactt cgttctggaa tcttatacct
     2521 ttacctttgt atggttccgg ttttctgagt gatctgattt tggaagccac ctgaccaagc
     2581 agttgtttat cgatagattc gaggatgatt gtagggggtt ttcctctctc gtttatggta
     2641 gtaactttca catcgggggg catttcaata aatatgttat gcgaataccc aagagaaagt
     2701 tcgagtaaat tattctgtgc atcggctttg tagccaacgc caataatttc ctgaaccagt
     2761 ttaaatcctt cggatacgcc gtgtaccatg tttgaaacaa gtgcgcgata aagtccgtgg
     2821 taggctctgg cctcatcgtt ttctctttcg agaatgatgt ggttgttttc cacttttatt
     2881 tttacggaag ggtttatctt ttgtttgagt tcgcctttag ggccttttac ggttacgata
     2941 ttatcatcag aaacgttgag ttgaacattc tgaggtaatg caatcggtag ttttccaata
     3001 cgtgacatgt tgttttctcc ttttattaac tgacgtaaca aattacttca ccgccaacat
     3061 tttgttgtct tgcttccttg tcggtcataa tgcctttgga ggttgaaata atagctattc
     3121 caagcccgtt taaaacacgc ggaagctcat cagcgttgac atattttctt aatccggggc
     3181 tgctgactct ttccagggta gagatggcgg atgttttcat cgtgggatgg tacttcagcg
     3241 ctatttttat ggttcccggt ttggggttgt cttcaaattt atagttcagg atatatcctt
     3301 tttcaaaaag aatttttgtt atactttttt tcagatttga agcaggaatt tccaccattc
     3361 tgtgattcgc catcacggca tttctaattc ttgtcaggta atctgaaata ggatccgtgt
     3421 tcatttttta gttttttatt gttactaatt attttaattt ttaccagctt gcttttttta
     3481 ctcccgggat gaggccggcg agtgccattt cgcgaaagtt gatacgcgag ataccgaact
     3541 gacgcatata tcctttaggg cgacccgtaa ttttgcagcg gttgtgcaaa cggacaggcg
     3601 aagcattttt aggcaatttt tgcaatgcga tatagtcgcc ttctttttta agctgcaatc
     3661 ttttttcctt gtatttttca acaagttgtt ctctttttaa ttcacgggct ttgattgatt
     3721 cttttgccat gttatttctg atttttaaag ggtaatccaa attcttttaa cagcgcgtat
     3781 gcttcctggt cgtttttggc ggaggtaacc atggtaatgt tcataccatt gattttgctc
     3841 accttatcga tggttatttc aggaaaaatt atttgttcgg taataccaaa ggagaaattt
     3901 ccctttccgt caaatccctt atcattgata ccgcggaaat cgcgaatacg ggggatggca
     3961 accgagatca gcctgtcgag aaattcgtac attttctcat tgcgcaatgt aacccttaca
     4021 ccaatgggca taccttttct gagtttgaag tttgatatgt cttttttgga tttggtttgc
     4081 actgctttct gacctgatac cagcgacatt tcgtcgatgc cggtttcgat cattttttta
     4141 tcagcaatag caccgccaac accttggttc aggcttattt tcagcaagcg tggcacctgc
     4201 atattattct tatattgaaa ttgtttgata agggcaggaa caatttcttt atcgtacttt
     4261 tcctttaatc ttggtttgta gtccattatt taattgtctc ctttttttct gatttttttg
     4321 aataccttac taattttcct gatttttcat caatttttcg gccggtccga gtaggttttc
     4381 ccgaagagtc gttaaccata aggtttgata cgtgtatgga agcttctttt ttcaagatac
     4441 cgcctttagt attttttgca ttaggtttgg tatgttttga aatcaggttg actccctcaa
     4501 caagcacttt atttttttca acgattacct ttaggacttt gccttgttga ccttttgaat
     4561 cgccggcaat aacggttact gtatcgcctt ttttaacatg tatttttggt cgcataacgt
     4621 tattttttat agcacttcgg gtgccagtga aataattttc atatattgtt tttcgcgcag
     4681 ttcgcgtgca acaggtccga atatacgggt acccctgagc tcatcgttgg ctgtaagtaa
     4741 tactacagca ttgtcgtcga aacggatata ggatccgtcg tttcttctga cttctttgct
     4801 ggttcgtaca ataactgctt tgcttacggt gccttttttg atgttaccgg ccggtatggc
     4861 gcttttaaca gttacaatga ttttgtctcc cacagaagca tatcttcttt ttgtgccacc
     4921 aaggactctg atgcagagga cttctttggc gccgctgttg tcggctacta ttagtctgga
     4981 ttcttgctgt atcattttat ttaacctttt cgataatttc tactaacctc cagcatttat
     5041 ttttgcttaa aggccttgtt tcagctattt ttacggtgtc gccaatgctg cattcatttt
     5101 ttgcgtcttc agccataaat ttggtatttt tcttcacaaa tttaccgtac ttggggtgca
     5161 ttacttttct ttcaacgttt acaacaatgg tattctgcat tttattgctg acaacaacac
     5221 ctattttttc ttttcttaaa ttacgttcca tggtggttta ttactttttg ctgtttttct
     5281 ttgtaactac ttcaacctgt tgagtgtcgc ctgccagatc ccgtttccgc agctcggtaa
     5341 gcatacgggc aatagttctc ttataatcct ttattttcat gggattttcc agaggagaaa
     5401 tggcgtgatt cattttcaat ttcaggtatt gctttttctc ttcttctaat ctttcacgaa
     5461 gttcgttcgt tgaaagttcg ctaatgactt gctgtttcat tgataattat ttttggtctt
     5521 caatgtaatc ttttctaata acaaacctgg tgtggaccgg taatttttgc gcacccagtt
     5581 cgagagcttt tttagcgata tcgaatggca caccgtctat ctcgaacatg atacggcccg
     5641 gggtaacgcg tgctacataa tattccaaag cgcctttacc ttttcccata cgaacttctg
     5701 caggtttttt ggttacaggt ttatcaggga atattcgtat ccataattgt ccttcacgtt
     5761 tcatgtgacg ggtgatggcc tgacgggcag cttcgatctg gcgtccggta atccaatatt
     5821 cgtcaagagt ttttatcgca aaagtgccga atgaaatctg
//
LOCUS       DAOSFP010000122         5851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12044_length_5851_cov_3.421498, whole genome shotgun sequence.
ACCESSION   DAOSFP010000122 DAOSFP010000000
VERSION     DAOSFP010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12044_length_5851_cov_3.421498"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="PLI16_04845"
     CDS             complement(<1..2084)
                     /locus_tag="PLI16_04845"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOH83919.1"
                     /translation="MKKIFTIIVLTLLSYSGFSEAFDITNYFVDMQMNANGSILVTET
                     IQVHFTEERHGIIRSMPFRYRQVDFPNRQRAERASLTADYELLIKDVAVDGFEFSTSI
                     EGDFLKIKIGSADKYINGNQTFVIKYLVWGALNKFSDRTEFYWNLIGHQWDCDIAAAG
                     FRIVLPKTLNLTNNDVLLFTGAEGSKNTAASYTVSPNEIKGALTSPLKANEGMTVAVK
                     FPNSAFTGTSIPIENLATNFYIDKLLTKININYDGSLTVEEKYSVVLMSPQASFIRDF
                     YTLRTNDSSTIKDHVLKEIKVNYISRGVNNAKFVSQNEGENKYISLYSETEKFSDKLE
                     VTYRYTVWGAVTFKNGIAEINWPLNGEMAGEPFKGFTAEISADPEINIEKTVFRYYKL
                     NKDHPISPEITFDKNKKTYVVNYSNVFSTDPIFLTQIISDEGFNKNLLPFEIYAKKHY
                     IKDFLTQIQINKNGSLHVSHQYLVKFKNPYSTENSFTANENFSYYDKSNDNKAYGTKL
                     PDWSFLSGYNRLIIKNLSASGIQDEYSLSNNLYNSVYWGNTAGTAQDTLYTYEYDVFG
                     LFTRKNGRYYLNKSLTSPFSEPIKTGTIQITLPENVHAADISTLFLVNNIESDKINFT
                     VKDNTITCKLSKPLFQGEVLFLDMSVPRDKLSVSCLLTWKIIWKNNKLLILPFIIFLL
                     LFILWYVFGRDK"
     gene            complement(2088..2642)
                     /locus_tag="PLI16_04850"
     CDS             complement(2088..2642)
                     /locus_tag="PLI16_04850"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH83920.1"
                     /translation="MTLYIIIGIAVLILLIAALYFNKFISYRNRIRNAWSDIDVQLKR
                     RYSLIPNLVESVKGYAAHEKGLFENVAKYRAQAENATTLKEHEAAENNMISVLKTILV
                     TVENYPALLANENFMKLQDSLIEVEDTIQNARRYYNALVRDNNTAVQSFPGNMFAGLF
                     SFKTADFFEIEPIERDVIKVEIKK"
     gene            complement(2760..4688)
                     /locus_tag="PLI16_04855"
     CDS             complement(2760..4688)
                     /locus_tag="PLI16_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83921.1"
                     /translation="MASKSTGKQKTISNRQKPVQAMQQKKSFLIPPWIIYTILAATAI
                     IYSRALFNGFASLDDDDYLFDNPYIKNFTFEAFKTIFTSFHLGNYHPLTTLTFWFEIH
                     WYGANPLPFHILNVLLHLINTFLVYQLVKKLSGQEITSLFTAALFALHPLHVESVAWI
                     SERKDVMYSMFYLSALLIYLQYLTNGHKSKHYLLCLLLFVCSLLSKSAAVTLPVLLIA
                     IDLYKRRKPDTKMWLEKIPFLLLSVLFGIIALLSQQGALKDVALEYSFFNRIFLFTYG
                     VMFYVVKLIVPFHLSAMHFYPVTHGGMLPWYCYASLPVLGFLAWLTVRKTSLRRETLF
                     GTGFFLITISVMLQIISVGNAITAERYTYISYIGLFYIAGQWMSALKKESVKRTALFI
                     SGIFIIAMAILTWNRIPVWKNGNTLFTDMIKKYPDSYLAYWMRGNYKNDVKDYQGALQ
                     DYNKTLQLHPNFAECLTNRGNIFNELKDYKAALQDLDRSIALDTTVAESFNNRGIAHN
                     GLGDTASALKDYNKAIQLDPELQNAYDNRGVLKAIMGDLTGALTDVNKALIIDPKDGE
                     TFSNRANIKAMMKDFKGSFDDYSIALQYNPKDNIVLFNRGLTRLNLNDTAGACDDWHR
                     AANLGNQAAGDVITNYCK"
     gene            complement(4956..>5851)
                     /locus_tag="PLI16_04860"
     CDS             complement(4956..>5851)
                     /locus_tag="PLI16_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOH83922.1"
                     /translation="GGLKLNFGNEDGAMELLHQLARGEGFGVIAGQGVHKMKEIFTEK
                     GWGDPKFLQDIGMENKGLEYSQYVSKESLAQQGGYAMTNKGPQHDEAWLIFMDMVNNQ
                     IPTFENKAEALYYFPMFRTWFGLQGLCKLPWNDVEPADNHTHAEPGKVPEHVDDYVTI
                     YKAVTGLDLDKDSMIIQSERVYQFQRVFNIRCGKGLRKNDAQPYRACGPVTIEEYESR
                     QERYDKQMKEKIGIDPEGKSTEEKLAITRKYREEQYEKLLDAVYKRRGWTSNGVPTIE
                     RLKSLGMDLPEVIEVVKDKQN"
BASE COUNT         1489 a         1167 c         1395 g         1800 t
ORIGIN      
        1 tttttgtccc ttccgaacac ataccaaagt atgaacagca aaaggaaaat gatgaatggc
       61 agtattagca gtttgttgtt tttccagatg attttccatg tgagcaagca cgaaaccgac
      121 aatttatcgc gcggaaccga catgtcgaga aagagtacct cgccctgaaa cagaggtttg
      181 ctgagtttac aggtgatcgt gttgtctttt accgtgaagt ttattttgtc ggattctatg
      241 ttgttgacca ggaataaggt ggagatatcc gcagcatgaa cattttcggg cagcgtgatt
      301 tggatagttc ctgttttgat gggttctgaa aaaggactgg tcagtgattt gttgaggtag
      361 tacctgccgt tttttcgggt aaacaatcca aaaacatcgt attcataggt atacagggta
      421 tcctgagccg tgccagctgt attgccccaa tacaccgagt tataaaggtt gttgctgagg
      481 ctgtattcgt cctgtattcc gcttgccgaa aggtttttga ttatcagcct gttgtatccg
      541 ctcaaaaagg accagtcggg gagttttgtc ccgtaagctt tgttgtcgtt acttttgtca
      601 taataagaaa aattctcgtt ggcagtaaag ctgttttcgg tgctatatgg gtttttaaat
      661 ttcaccaggt attggtgtga cacatgcagg ctgccgtttt tgttgatttg tatctgggtt
      721 agaaaatctt tgatgtaatg ttttttcgca tagatttcaa acggcagcag gtttttgttg
      781 aagccttcgt cgcttattat ttgtgtgaga aaaatggggt cagtggaaaa cacattggaa
      841 tagttgacta cataagtttt tttgtttttg tcaaaggtta tttcgggact gatgggatgg
      901 tccttgttta gtttgtaata cctgaagaca gttttttcaa tattaatttc gggatcggca
      961 gaaatttcag cggtgaatcc tttgaagggc tcaccggcca tttcaccgtt gagaggccaa
     1021 ttgatttctg caatgccatt tttgaacgta acggctcccc acacggtgta ccggtatgtt
     1081 acttccagct tatccgaaaa tttttctgtt tcggaataca ggctgatgta tttattctcg
     1141 ccttcgtttt gtgatacaaa tttggcattg ttcacgcctc gcgagatata atttacttta
     1201 atttctttga gtacatggtc ttttattgtg gaggaatcat ttgtacggag ggtataaaag
     1261 tctctgataa aagaggcttg cggcgacatc agcaccaccg aatatttttc ctctacggta
     1321 agggaaccgt cgtagttaat gttgattttt gtcagcagct tatcgatata aaaatttgtt
     1381 gccagatttt caatgggaat gctggtgccg gtgaaggcgg agttggggaa ttttacggcc
     1441 acggtcatgc cttcattggc tttcagcggg ctggtgaggg cgcctttaat ctcgtttgga
     1501 gataccgtgt atgaggctgc cgtgtttttt gagccttcgg ccccggtaaa cagaaggacg
     1561 tcgttgtttg tcaggtttaa cgttttgggc aaaacaatcc tgaatcctgc tgctgcaatg
     1621 tcgcaatccc attgatgtcc gatcaggttc cagtaaaatt cggtgcggtc gctgaacttg
     1681 ttcaaggcgc cccaaacaag gtattttatt acaaaagtct ggttcccgtt gatgtattta
     1741 tcggctgaac cgatctttat tttcaggaaa tcgccttcta tagaggtgct gaactcaaaa
     1801 ccatcgacag cgacatcttt tatcaataat tcataatcgg cggtcaggct ggctctttcg
     1861 gcccgctgcc tgtttggaaa atctacctgc cggtatctga aaggcataga acggatgatg
     1921 ccatgccttt cctcagtaaa gtgaacctgt atggtttccg ttaccagtat ggatccgttg
     1981 gcgttcatct gcatatccac aaagtagttt gtgatgtcaa atgcttcgga aaaacccgaa
     2041 tagcttagca gggttaaaac aatgatggta aatatctttt tcatgtacta tttttttatt
     2101 tccaccttaa ttacgtcgcg ttcgatgggt tcaatttcga aaaaatcggc tgttttaaaa
     2161 ctgaaaaggc cggcaaacat atttccgggg aaggactgca cggccgtgtt gttgtcgcgc
     2221 accaacgcat tataataccg gcgggcattc tgtatggtgt cttccacctc aatcaacgaa
     2281 tcctgcagct tcataaagtt ttcattggcc agcagggcag ggtagttttc tacggtaacc
     2341 aggatagttt ttagtacgga tatcatattg ttttcggcgg cttcgtgttc tttcagcgtt
     2401 gtggcgtttt cggcctgtgc acggtacttc gccacgtttt cgaaaagacc tttttcgtgg
     2461 gcggcatagc ctttcacgga ttcgacaagg ttgggtatca gcgaatagcg cctttttaac
     2521 tgcacgtcga tatcgctcca ggcattgcgt atcctgttgc ggtaagaaat gaatttgttg
     2581 aagtacagcg ctgcaataag cagaattaat acagcgattc caatgataat gtataatgtc
     2641 ataaggtgta atttgttatt ccaaaaataa tgaaattttc agcttgttct gaatgtggag
     2701 gatttcttta ataatttccc gaagatttct gagattatca aagaattttg cgtgaagggt
     2761 tatttacaat aatttgtaat cacatcgccg gcagcctggt tgccaagatt tgcagcgcgg
     2821 tgccagtcat cacaggcgcc agcggtgtcg ttcaggttga ggcgtgtcag tccccggttg
     2881 aagagtacga tattgtcttt ggggttatat tgtaaagcga tgctgtaatc atcaaacgag
     2941 cctttaaagt ctttcatcat ggctttgatg ttggcgcggt tgctgaatgt ttcgccatct
     3001 ttagggtcta tgataagagc cttattcaca tcggtcaatg ctcccgtaag atcccccatg
     3061 attgccttca gcacgcccct gttgtcgtaa gcattctgta attccggatc aagctgtatg
     3121 gctttgttgt aatcctttag tgcagaggcc gtatcgccca gtccgttatg agctatacca
     3181 cggttgttaa aagattcagc cacagtggtg tcaagcgcaa ttgaccggtc caggtcctgc
     3241 aaggcagctt tataatcttt cagttcgtta aaaatatttc cccggttggt caggcattca
     3301 gcaaagttgg gatgcagctg aagggttttg ttataatcct gcagagctcc ctggtaatcc
     3361 ttcacatcat tcttgtaatt tccccgcatc caataagcca ggtaactgtc ggggtacttt
     3421 tttatcatat cggtaaacag ggtattgccg tttttccaca cggggatacg gttccaggta
     3481 aggatggcca tggctatgat aaatatgccg ctgatgaaga gtgctgtcct ttttactgac
     3541 tcttttttca gagcactcat ccattgaccg gcgatataaa ataatccgat ataagatatg
     3601 taagtgtagc gttcggcagt gatggcgtta cccaccgaaa tgatctgcag catgaccgaa
     3661 atagttatca ggaaaaaacc ggtgccaaaa agtgtttcac gacgcaaaga tgtctttctt
     3721 accgtgagcc acgccaaaaa accgagtaca ggcagcgaag cgtaacaata ccacggcagc
     3781 atgccgccat gagttaccgg ataaaaatgc atggccgaca ggtgaaaggg gacaataagt
     3841 tttacgacat aaaacatcac gccgtaggta aataaaaaaa tcctgttaaa aaatgagtat
     3901 tccaacgcaa cgtcttttaa agcgccttgc tgtgaaagta atgcaatgat gccgaacagt
     3961 actgataaca acagaaaagg tattttttca agccacattt ttgtatccgg ttttcttcgt
     4021 ttgtaaaggt caattgctat caacaacacc ggcagggtaa cggcagcgga ttttgaaagc
     4081 agggaacaca cgaacaaaag aaggcacagc aggtagtgtt tgcttttatg tccattagtg
     4141 aggtattgga ggtagatgag cagggcagaa agataaaaca ttgaatacat aacgtcttta
     4201 cgctcagata tccaggcgac agattcaaca tgcaggggat gcaaggcaaa caaggccgca
     4261 gtgaataaag atgtaatctc ttgtccgctg agttttttga caagttggta caccaggaaa
     4321 gtgttgatca gatggagcag cacattaagg atatggaagg gcagcgggtt ggctccatac
     4381 caatgaatct caaaccaaaa agtaagtgtg gtcagggggt ggtagttgcc caggtgaaag
     4441 gaagtaaaaa ttgttttaaa tgcctcgaaa gtaaaattct ttatgtatgg gttgtcgaaa
     4501 aggtaatcgt catcatcgag gctggcgaat ccattgaata aggctctgga ataaatgatt
     4561 gcagtggcag ccagtatggt ataaattatc catgggggaa taagaaatga ttttttttgt
     4621 tgcatggcct gtaccggttt ttgccggttt gaaatggttt tctgttttcc tgttgacttg
     4681 ctggccataa gttgttttat tacactatag gttgttcaca aaaataataa agaaaaacta
     4741 ttccttacaa gggcaagaaa acaagaagag gctgacacaa aatacagttt tttatatttt
     4801 tctctctgtg gctctctgtg aaccgcaata gcggtctctg tgaatctcag tgtaataata
     4861 aattaaagaa ttacacagag taccacggag tacacacaga gttacacaga gtacttttga
     4921 gatagcctct ttttcaaata cacagcattg atttgttaat tctgtttgtc ttttaccact
     4981 tcaatcactt ccggcagatc catgcctaat gattttagtc tttcgatggt gggcactccg
     5041 ttggaggtcc aaccacggcg tttgtacacg gcgtccagca gtttttcgta ctgttcttcg
     5101 cggtatttgc gggtgatggc caatttttct tcggttgatt ttccttcggg gtcgatgccg
     5161 attttttctt tcatttgttt gtcgtagcgt tcctggcggg attcatattc ttcgatggtt
     5221 accggaccgc aggcacggta tggctgggca tcgtttttgc gcagaccttt tccgcaacgg
     5281 atattgaaaa cccgctggaa ctgatatacc cgctctgatt ggatgatcat agaatcttta
     5341 tcaaggtcaa ggccggtaac cgctttataa atggttacat aatcgtcgac gtgttcgggg
     5401 acttttccgg gttcggcatg ggtgtggtta tcggccggtt ccacgtcgtt ccagggcagt
     5461 ttgcataaac cctgcagtcc aaaccatgtg cggaacatcg ggaagtaata cagggcttcg
     5521 gctttgtttt caaaggtggg tatctggttg tttaccatgt ccataaaaat cagccaggct
     5581 tcgtcgtgtt gaggcccttt gttagtcatg gcgtagccgc cctgctgtgc cagcgattct
     5641 ttggaaacgt attgggaata ctccaggcct ttgttttcca tgcctatatc ttgcaggaat
     5701 tttggatcgc cccagccttt ttcggtaaat atttctttca ttttatgtac accttgtccg
     5761 gcaataacgc caaagccttc accgcgcgcg agctgatgga ggagttccat ggcgccgtct
     5821 tcgttgccga aatttaattt caatccgccg g
//
LOCUS       DAOSFP010000123         5824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12128_length_5824_cov_3.654360, whole genome shotgun sequence.
ACCESSION   DAOSFP010000123 DAOSFP010000000
VERSION     DAOSFP010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12128_length_5824_cov_3.654360"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1785)
                     /locus_tag="PLI16_04865"
     CDS             complement(115..1785)
                     /locus_tag="PLI16_04865"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOH83923.1"
                     /translation="MKTDIEIAHEAKMVHIREIAAQLGIAEDDLQYYGKYKAKLPLKY
                     INPEKVKSSKLILVSAISPTPAGEGKTTVSIGLAQGLNRIGKKTTVVLREPSMGPVFG
                     VKGGAAGGGYSQVLPMEDINLHFTGDFGAIEKAHNLLAAIIDNNIQNKKNNLFIDPRT
                     VLWKRVMDMNDRSLRKIIVGLGGTMSGVPRETGVDITAASEIMAILCLSDNLLHCKEK
                     MGNIFVGYTYDKKPVYARDLKANGAMAALLKDAILPNLVQTIENTPAIIHGGPFANIA
                     QGTNSVIATRMGLSLSDYVVTEAGFGFDLGAEKFLDIKCRYSGLDPEAVVLVATVRAL
                     KYHGGGDLKTLKEPNPGLVRNGLPNLDKHIENMKLFNLCPVVAINRFASDTDEEIETV
                     KERCRQLGIPACAVNVWSEGGAGALELADMVSKVADMCTARYSPLYELDWSVEKKIET
                     ICKKIYGAQHVDYLPKAKKDLQKVYNLGLDKLAVCIAKTQKSLSDNPELLGRPKDFVV
                     TVREIEVAAGAGFIVPITGEIMRMPGLPEIPSAEFIDIDENGNIVGLS"
     gene            1920..2792
                     /locus_tag="PLI16_04870"
     CDS             1920..2792
                     /locus_tag="PLI16_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83924.1"
                     /translation="MKVSGFTFIKNALIYDYPVVEAIRSVLPLCDDFVVAVGKSDDDT
                     YRLINDIDRHKIKIIETVWDETLREGGKVLARETDKAFAGVSADADWAFYIQGDEALH
                     EKYYDIIRESMTKYKDHKEVDGLLFKYRHFYGSYDYVGASPKWYKHEIRVIKNDKSIY
                     SFRDAQGFRKCDNQLLRVKPVDAYIYHYGWVKEPEAMQRKQEHFNKYWHDDNWVERHI
                     EKTEKFDYSKNPDFLVPFTGTHPQVMQARIKAKNWKFDYDASYDKRSLKTRVKSMLEK
                     YLGLDFSYKNYKII"
     gene            2849..3706
                     /locus_tag="PLI16_04875"
     CDS             2849..3706
                     /locus_tag="PLI16_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH83925.1"
                     /translation="MKKTLYFVALAIIMTIPVSVFSQNEDAEGCQNHPLFNQMNNFFI
                     NACELREFEAFKFPLENSTEDDTKTETVEGKYYHYEYLLKEGADQPSALQVYRNYENA
                     LKNINATIVSKVYEPGNSNNFLCAKIAKGVLETWVKIDAGDGARTDYYITIVEKEIMK
                     QVIQANEMLNALNSDGYIALNILFDTGKSTVKAESEPIIEQIFLLLQSSPSLQVSIEG
                     HTDNTGNSESNQKLSEERAKAVMDALIAKGIKKERLSFVGWGQQKNIADNRTEEGRAK
                     NRRVEIVKK"
     gene            complement(3809..5071)
                     /locus_tag="PLI16_04880"
     CDS             complement(3809..5071)
                     /locus_tag="PLI16_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH83926.1"
                     /translation="MADDFQNKETLRVVVAGNVDDGKSTLLGRLLFDSRSIFDDQLMA
                     LCQASEKRGDDNVNLALLSDALHAEQEQGITLDVAHHYFSTARRQFIFIDTPGHIQHT
                     RNMITGASTADLILMLIDARKGIAEQTRRHTFIASLLGIKHMMVAVNKMDLVDYREDI
                     FELIRSNLGNFASKLDFQTIDYIPICALHGDNIVNASLKMPWYKGKTLLNQLENFSCT
                     TPAQITGGRFPVQYVLRPVSNDYQDYLAYAGIVAGGEFKTGQKVIVLPSKTSSYIKSI
                     NMGENSLEKASSPMSVSIQLTDDLDINRGDLIVTGDDLPVITQEFDAMIFWCSTAKLL
                     PGNHYLLMQVAREDRCSIKSISYKIDINTGNKIDGVGEAGLNDIVRVSIKTLHGLYID
                     KYTANHTTGSLILIDETTNETVAAGMII"
     gene            complement(5094..>5824)
                     /gene="cysD"
                     /locus_tag="PLI16_04885"
     CDS             complement(5094..>5824)
                     /gene="cysD"
                     /locus_tag="PLI16_04885"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOH83927.1"
                     /translation="LLHVDTGHNFPETLDFRDKMVKKTGVRLMVVYVQDTINSGRAKE
                     ETGYNASRNSLQQITLLDTIEKFSIDAALAGSRRDEEKVNAKERFFSIRDKSGHWDPK
                     SQRSEFWNIYNGKKNQGEHFRVFPLANWTEMDVWQYILRENIELPSLYFSHDRECFIR
                     DGVIFAWADFIKLKAGEQVQTLHVRFRTIGDITCSGATLSTAKNIGDIIKELQTTHDT
                     ERGHRSDDKRTDTTPEDRKKEGYF"
BASE COUNT         1628 a         1321 c         1267 g         1608 t
ORIGIN      
        1 gatcaggata ttcagcaagg aaaacaatga agtttttacc cgcagcattc gtaacaagta
       61 ctatgaatta atgaactact gatgagtatt tcagcactgt ttttctcagg cctgttacga
      121 taatcctaca atatttccat tttcatcaat atcaataaat tctgctgaag gtatttcggg
      181 caggcccggc atacgcatga tttcgccggt aatagggaca ataaaccctg ccccggcagc
      241 aacttctatt tcgcgcacgg tcaccacaaa atcttttggt ctgcccaaca gttcgggatt
      301 gtctgacaat gatttctggg ttttggcaat acataccgcc agtttatcca ggcccaggtt
      361 atagactttc tgaaggtctt ttttggcctt tggcaaatag tcaacgtgct gtgcgccata
      421 gattttttta caaattgttt caatcttttt ttcgacagac cagtctaatt catatagagg
      481 gctgtatctg gcggtgcaca tatcggccac tttcgacacc atatcggcca attcaagcgc
      541 tcctgcccca ccttcactcc agacatttac cgcacacgca ggaatgccca gttggcggca
      601 gcgttctttt actgtttcaa tttcttcgtc ggtatcgctg gcaaatctgt tgatagccac
      661 caccggacaa aggttgaaaa gtttcatatt ttcgatgtgt ttgtcaagat tgggcaggcc
      721 atttcttacc agcccgggat tgggttcttt caaggtcttc agatcgccgc cgccatggta
      781 cttgagcgcc ctcaccgtgg ccaccagcac caccgcttcc gggtccagcc cggaataacg
      841 gcatttaata tcaagaaact tttcggcgcc cagatcaaaa ccaaaaccgg cttcagtaac
      901 cacataatcc gacagggaca agcccatgcg tgtagcgatc accgagttgg taccctgggc
      961 aatattggca aaaggccctc cgtgaatgat ggcgggggta ttttcgatag tctgcaccag
     1021 gttaggcaaa atggcatcct taagcagggc tgccatagcg ccattggctt tcaggtcgcg
     1081 ggcatacacg ggttttttgt cgtaggtata tccaacaaaa atgtttccca ttttttcttt
     1141 gcaatgcagc aaattatccg aaaggcagag tatagccata atttcggatg cagcggtaat
     1201 atcaacgcct gtttcgcggg gtacgcccga catggtgcca cccagtccga caataatttt
     1261 tcgcaacgaa cggtcgttca tatccatcac gcgtttccac aaaacagtcc tcgggtcaat
     1321 aaataggtta ttctttttgt tctgaatatt attgtcgatg atagcagcaa gcaggttgtg
     1381 tgctttttcg atagccccga aatcacctgt aaagtgcagg ttgatatctt ccatgggcag
     1441 cacctgtgaa tagccgcccc ctgcagcgcc gcctttcaca ccaaaaacag gacccattga
     1501 cggctccctg agcaccaccg tggttttttt tccaatacgg ttcagcccct gtgccagacc
     1561 tatggaaaca gtggttttcc cttcgccggc cggagtaggc gaaatggcag aaaccaggat
     1621 gagtttactg ctcttaactt tttcagggtt gatatatttc agcggcagtt tggccttgta
     1681 cttcccgtaa tactgcagat catcctcggc aatgccaagc tgagcggcaa tctcacgaat
     1741 gtgtaccatt ttggcttcat gtgcaatttc gatgtctgtt ttcatagttt ccgtaattta
     1801 atgttagtat aactattcaa aaataatcaa aatttttcat atagtcatat tgaagaatgc
     1861 tatctgattc accttctttt taaaatatta tgtacgtttg cagaaaataa tatcccgtaa
     1921 tgaaagtcag cggatttact tttattaaaa acgccctgat atacgactat ccggtggtgg
     1981 aagccatacg ctcggttttg ccattatgtg atgattttgt cgtggctgtg ggtaaatccg
     2041 acgatgatac ctaccggctg attaacgata tagacagaca taaaataaaa atcattgaaa
     2101 cggtatggga tgagaccttg cgtgaaggcg gaaaggtact tgccagggaa acagacaaag
     2161 cttttgccgg agtttccgcc gatgcagact gggcttttta catacagggc gacgaagccc
     2221 ttcacgaaaa atactatgac atcatccgcg aaagcatgac aaaatacaag gaccataaag
     2281 aagttgacgg cctgttattc aaataccgcc atttttacgg ttcgtatgat tatgtcggag
     2341 cttcgcccaa atggtacaaa cacgagatac gggtgattaa aaatgataaa tccatttatt
     2401 cgttccgcga cgcacaggga ttccgcaaat gcgacaacca gctcttgcgc gtaaaacccg
     2461 ttgacgctta catttatcat tacggatggg taaaagaacc ggaggctatg caacgcaaac
     2521 aggagcattt caataaatac tggcacgatg acaattgggt ggaacgccac atcgagaaaa
     2581 cagaaaaatt tgattactca aaaaatccag attttcttgt tcccttcacc ggaacacatc
     2641 cccaggtgat gcaagccagg ataaaagcca aaaactggaa atttgattac gacgcaagtt
     2701 atgataagcg ctcgttgaaa accagggtaa agagtatgct cgaaaagtat ctggggcttg
     2761 atttcagcta taaaaactac aaaatcatct gacctgcatc agaagatttt aaaaactcga
     2821 atagtaacta cattgaaagg agcattttat gaaaaaaaca ctttattttg tagcattggc
     2881 gataataatg acaatacctg tttcggtatt ttcgcagaat gaagatgctg aaggatgcca
     2941 aaatcatccg ttatttaacc agatgaataa tttctttatc aatgcatgtg agcttcgtga
     3001 atttgaggcc tttaaatttc cgttggaaaa cagcaccgag gatgacacga aaacggaaac
     3061 agtggaaggc aagtattatc actatgagta tttattaaaa gagggagcgg accagccatc
     3121 tgcgttgcaa gtttacagaa actatgaaaa tgcactgaag aatattaatg ctaccatagt
     3181 ttcaaaggtt tatgagcccg gcaattcaaa taatttttta tgtgcaaaaa ttgcaaaagg
     3241 tgttttagaa acctgggtga aaattgacgc aggagatggc gcccggacag attactacat
     3301 tacgatagtt gaaaaagaaa ttatgaagca ggttattcag gccaacgaaa tgctaaatgc
     3361 cctaaattcc gatggatata ttgccctgaa catacttttt gacaccggaa aatcaaccgt
     3421 caaagcagaa tcagagccca tcattgaaca gattttttta ttgctccagt ccagtccctc
     3481 cttacaggtt tcaatagaag gccataccga caatacaggc aatagcgaat caaaccaaaa
     3541 actatctgag gagcgcgcaa aagctgtaat ggatgccctt attgccaaag gcataaaaaa
     3601 agaacgatta tctttcgttg gctggggtca gcaaaaaaat attgccgaca accgcacaga
     3661 ggaaggaaga gcaaagaaca gacgggttga aattgtgaag aaatagaaat tttcatgatg
     3721 cagttgcgga agcaggaagc ttatcaacaa tgttgtgcat ggccatcggc cataatttta
     3781 tgcctgaaac cgcaagcaac aacccatgtc agataatcat gccggcggcc acggtttcgt
     3841 tagtggtttc atcgatcaga atcaggctgc ctgtagtatg gttggccgtg tatttatcaa
     3901 tataaagacc atgtaaggtt tttatggaca ccctgacgat atcattcaat ccggcctctc
     3961 ccaccccatc aattttgttg cccgtgttga tatcaatttt ataggaaata ctctttatcg
     4021 agcacctgtc ctcacgggca acctgcatca gcaggtaatg atttcccggt aaaagttttg
     4081 ccgtgctgca ccagaaaatc atggcgtcaa actcctgtgt aatcacgggc aggtcgtcgc
     4141 cggttactat caggtcgccg cggttgatat ctaaatcatc agtaagttgt atgctgaccg
     4201 acatcggcga tgaagccttt tccagggagt tttctcccat gttaatagac ttgatatatg
     4261 aacttgtttt cgatggcagc actatcacct tttggcccgt tttaaactcg ccgccggcca
     4321 caatacccgc ataagccagg taatcctgat aatcattgct gacgggcctc aacacatact
     4381 gaacaggaaa ccgcccaccg gtgatttgag ctggtgtagt gcatgagaaa ttttcgagtt
     4441 ggttcaacaa ggtttttcct ttgtaccagg gcatcttcag cgaagcgttg acaatattat
     4501 cgccatgaag ggcgcatatg gggatataat caatagtctg aaaatcgagt ttagaggcaa
     4561 aattgccgag atttgacctg ataagctcaa aaatatcttc gcgataatcc acaaggtcca
     4621 ttttattcac cgccaccatc atgtgcttta tgccaagcag tgatgcgata aaagtgtgcc
     4681 ggcgggtttg ttccgctatg cctttgcggg catcgatcag catcaggata agatcggctg
     4741 tggaggcgcc ggtaatcata ttgcgtgtgt gctgaatatg tccgggagtg tcgataaaaa
     4801 tgaattgtcg ccgggctgtg gaaaaataat gatgagcaac atccagagtg attccttgtt
     4861 cctgttcggc atgcagggca tcggaaagca aagccaggtt cacattatca tcacccctct
     4921 tttcactggc ctgacaaagc gccatcagct ggtcatcaaa tattgacctg gaatcaaata
     4981 acaaccgccc cagcagtgta ctttttccgt catccacatt gcctgcaacc actacacgaa
     5041 gtgtttcctt gttctgaaaa tcgtctgcca ttgttgtcaa tattgttagg taattaaaag
     5101 tatccctctt ttttacggtc ttcaggggtt gtatccgtgc gtttgtcgtc gctgcggtgc
     5161 cctctttcgg tgtcgtgggt agtttgcagt tcctttatga tgtcgccgat attttttgct
     5221 gtcgacaggg tggctccgct gcaggtaata tctccgatgg tgcgaaaacg cacatgcaag
     5281 gtctgtacct gctctcctgc tttcaattta ataaaatccg cccaggcaaa tatcacaccg
     5341 tcgcgtataa agcattcacg gtcgtgagaa aaatacaacg acggcaactc aatattttca
     5401 cggagaatat actgccatac atccatttct gtccagtttg ccagaggaaa caccctgaaa
     5461 tgttctccct ggtttttttt gccattgtaa atattccaga attcggagcg ttgacttttt
     5521 ggatcccaat gcccggattt gtcccttata gagaaaaacc tttctttggc attgactttt
     5581 tcttcgtccc tgcgggagcc tgccagggca gcatctatcg aaaatttttc aatcgtatcc
     5641 agcagggtaa tctgttgcag ggaattgcga ctggcattgt agccggtttc ttcttttgcg
     5701 cgtcccgaat tgatagtatc ctgcacgtaa accaccatca gccttacccc ggtcttttta
     5761 accatcttat ccctgaaatc tagtgtttcc gggaaattat gcccggtatc cacatgcaac
     5821 agcg
//
LOCUS       DAOSFP010000124         5779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12257_length_5779_cov_4.059748, whole genome shotgun sequence.
ACCESSION   DAOSFP010000124 DAOSFP010000000
VERSION     DAOSFP010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12257_length_5779_cov_4.059748"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLI16_04890"
     CDS             <1..194
                     /locus_tag="PLI16_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83928.1"
                     /translation="YIQPLFVWNNPVMLSGTVFSNLRYKTIKDLKTQNKDEKITTYRM
                     QGTYLKEITAPVVLGSNKL"
     gene            191..1183
                     /locus_tag="PLI16_04895"
     CDS             191..1183
                     /locus_tag="PLI16_04895"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOH83929.1"
                     /translation="MITDFPHIHAAHCETGTLSALLRYRGMEMSEPMIFGTGSGLYFA
                     YMPYLKRNGIPFISYRIMPGWLSRIFKNRFQVEIIKKQYRTQGAAMDDLDNLLEQDIP
                     VGLVTNLFFIPYFPDSYRFHFNNHHMIVYGKENGHYLVSDPIFDVPTTISYEDLCTAR
                     FAAGLRTLKGDLFYIGNTPQDYDLKKIIHRAIRYTSKNLLTPMPLNGYRGIRYLSKRV
                     KQWPRKMDEKKAKRYMGNIIRMLEEVGTGGSGFRYMYAAFLQEAANVTNKDLLLEASA
                     GMTLAGDTWREFAYQAATICKTDRPGEQMYHEASGILLRCSETEESVFRKLSIL"
     gene            1193..1870
                     /locus_tag="PLI16_04900"
     CDS             1193..1870
                     /locus_tag="PLI16_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH83930.1"
                     /translation="MYKETILQVEKLSKYHKGNDYPAIDNVSLAIKRNEIFGLLGPNG
                     AGKTTLISVMCGLYKPDSGHVLVNGTLLHQHLKSLKKIIGIVPQDIALYPTLTARENL
                     LFFGHVYGLPRRFLDDKIDQCLTILELEKSKNKKINTFSGGMKRRINIAAGLLHEPQL
                     LFLDEPTVGIDVHSKKVIIDYRKTSTKMALPSCTHHTLLMKPKIFAVISPSWTREKLL
                     QAGNPLI"
     gene            1943..3232
                     /locus_tag="PLI16_04905"
     CDS             1943..3232
                     /locus_tag="PLI16_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH83931.1"
                     /translation="MKTFLASIKKEFLVLLRDRSGLGILFIMPLALVVIMALVQDGPY
                     KNFQKSEYPMIFVDNDRDTVGAVIERNIRKAHIFKINTDINGVPATEKTAREAVSQGK
                     YRAGVVVPAGLSKYLRRNARILVAKTFSGMPADEASLVPEAPDSMNVKYFLDPLTTFS
                     FKNTIVYILEKSLSSLISQTLTTNYYKHFENFYPIENKINFDSCQIIGMQEISASSTY
                     NEHVVFNSVQHNVPAWTMFAMFFIFLPLAGSIIKERDYGTFIRLRFMPGSFSTLIAGK
                     VFLYTLVGCIQFLMMLTAGRCLLPALGLPALIIGNSYAAIAATVVAASLAATGFGVLI
                     GTFFPTHQQMVTFGSISVIILAALGGIFVPIYIMSKTMAKVSKYSPLEWGLNAFNEIF
                     LHQGTFINIYIDALKLLLFSAVAFLLSFLYYKFKRIH"
     gene            3248..3514
                     /locus_tag="PLI16_04910"
     CDS             3248..3514
                     /locus_tag="PLI16_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOH83932.1"
                     /translation="MTTLTDEIKKNIIEELNLTHELDEISHDTPLFSNRHTHLDSLDA
                     LRIIVMLEKKYGIKIKDLSQRKKILYSLDAIAKLVEEHRQQSSQ"
     gene            complement(3574..4080)
                     /locus_tag="PLI16_04915"
     CDS             complement(3574..4080)
                     /locus_tag="PLI16_04915"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH83933.1"
                     /translation="MSDTADFWIAQLQLQPHPEGGFFKEVYRSEEKISADFLSTRYQA
                     ERCFATSIYFLLKSGQISAFHRLQSDEIWHFYTGSPIRVYIIDQVGVLSETLLGPDVL
                     NKEVFQLVIPHGCWFAAEVTREDSYSLIGCTVAPGFDFHDFELATKAGLTACFPAYSG
                     LFEKMCIR"
     gene            complement(4088..4837)
                     /locus_tag="PLI16_04920"
     CDS             complement(4088..4837)
                     /locus_tag="PLI16_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH83934.1"
                     /translation="MKINLPVSFFLCFLLGFSVVRAQETNETRPGVDREIKGVVIAGM
                     NLSQVDGDEVYGFKKMGACVGVGGILPLPKRFSLSLEILYDEKGAYQKLPPSAVDSLQ
                     LPYYSLKWNYLTAPLMVHYEDRNTWTFGLGVSWGRMVTFKEKEWNVTKPWERPDTITY
                     KTKNDFNIIADLRFRIWKHLKFNIRYSYSLAPVRKRDYVNPVTFETWSRRQYNSVITI
                     RLLYYFNEKYLPDRFTFKKKSKKKNSQTKNN"
     gene            complement(5071..>5779)
                     /gene="thiL"
                     /locus_tag="PLI16_04925"
     CDS             complement(5071..>5779)
                     /gene="thiL"
                     /locus_tag="PLI16_04925"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOH83935.1"
                     /translation="EELYAGIKLACEKYNVDLVGGDTTSSTSGMFLSLTVIGVVSADK
                     VVYRNTANENDLICVSGDLGSAYAGLLLLEREKAGFLANPGMQPDLEGYDYILERQLK
                     PEARKDIVKLFAENDVMPTSMIDISDGLASDILHICHDSGKGCAIYEERIPVDMSTIT
                     MCDSVKIDPTTAALNGGEDYELLFTIAQNDYEKIKTFAEVSIIGHITDSNAGTNLVTR
                     SGQMVPLRAQGWNHYRK"
BASE COUNT         1666 a         1372 c         1222 g         1519 t
ORIGIN      
        1 cttatatcca gcctttgttt gtatggaaca accctgtaat gctcagcgga acagtattct
       61 cgaatttgag gtacaagaca ataaaggatt taaaaacaca aaacaaagac gaaaaaataa
      121 ccacgtaccg tatgcaggga acctacctga aagaaattac cgcaccggtt gttttaggaa
      181 gtaacaagtt atgataacag attttccgca tatacacgcc gcccactgcg aaacaggcac
      241 cttatccgct ctgctaagat atcggggaat ggagatgagt gaaccgatga tctttggcac
      301 cgggtcagga ttatattttg catacatgcc ctatcttaaa cgcaatggca ttccgtttat
      361 ctcctaccgt ataatgcccg gatggctgtc gcgaatattt aaaaacaggt ttcaggttga
      421 gataatcaaa aaacaatacc ggacgcaagg tgccgccatg gacgatctgg acaacctgct
      481 tgagcaggac attcccgtgg gtttggtaac caatcttttc tttatcccct attttcccga
      541 ttcgtaccgt tttcatttca acaaccacca catgatagtt tacggaaaag aaaacggaca
      601 ctaccttgtc agtgatccta tttttgatgt ccccacaacg atctcgtacg aggacctatg
      661 caccgcacgt tttgctgccg gattaagaac gctgaagggc gacctttttt acatcggcaa
      721 caccccgcag gattatgact tgaaaaaaat cattcaccgg gccatccggt acacaagcaa
      781 aaacctgctt acgccaatgc cgttaaacgg atacaggggc atacgctacc tgtcgaaacg
      841 cgttaaacaa tggccaagga agatggatga aaaaaaagcc aaacgttata tgggcaacat
      901 catccgcatg ctcgaagaag taggtaccgg cggatcggga tttcgttata tgtacgcggc
      961 ttttctgcaa gaagccgcaa acgtaaccaa caaagacctg ttgctggaag cctcggcagg
     1021 aatgacgctg gccggagaca cctggcgcga atttgcctac caggcggcaa ccatctgcaa
     1081 aaccgaccgg cccggcgagc aaatgtatca cgaggcctcc gggatattgt tacgctgctc
     1141 ggaaaccgaa gaatccgtct tcagaaaact atcaatactt tagacgctta caatgtacaa
     1201 ggaaacaata cttcaggttg aaaaactatc gaaatatcat aaaggcaacg actatcccgc
     1261 tattgacaat gtttctcttg cgataaagcg taatgagatc tttggattgc tgggccccaa
     1321 cggagcaggc aaaaccacac tgatttcggt catgtgcggc ctgtacaaac ccgacagcgg
     1381 gcatgtgctg gtcaatggca ccctgttgca tcagcacctg aaatcactaa aaaaaattat
     1441 aggtatcgta ccgcaggaca tcgcactgta ccccaccctg acagcccggg aaaatttgct
     1501 ttttttcggc catgtgtacg gtttgccccg caggtttctt gatgacaaaa ttgatcaatg
     1561 ccttaccata ctggaacttg aaaaatcaaa aaacaaaaaa attaacactt tttcgggagg
     1621 tatgaaacgc cggatcaaca tcgcggccgg actgctgcac gaacctcaac tgctgtttct
     1681 cgacgagcct accgtcggca ttgacgtaca ttccaaaaag gtgattatcg actaccgaaa
     1741 gacatcaaca aaaatggcac taccatcctg tacacatcac accttattga tgaagccgaa
     1801 gatttttgca gttatatcgc catcatggac cagggaaaaa ttgttacagg cgggaaaccc
     1861 gctgatttga tcagcagcta taaaaatacc aaagacctgg aagatgtttt tctgcaacta
     1921 actgaaaaat ccaattccta gtatgaagac attccttgca tcgataaaaa aagaattcct
     1981 ggtgctgttg agagaccgct cggggctggg gatacttttc atcatgccgc tggcgctggt
     2041 cgtcatcatg gccctggtgc aggacgggcc ttacaagaat tttcaaaaat cagaataccc
     2101 gatgattttt gtggacaacg atagagacac cgtcggagct gtaatcgaaa gaaacatcag
     2161 gaaggcccac atttttaaga ttaacaccga tatcaacggg gtacctgcca ccgaaaaaac
     2221 ggccagggag gccgtttcgc aaggaaagta ccgggcagga gtggtagtac ctgccggact
     2281 ttcaaaatac ctgcgaagaa atgcccgtat attggtagcc aaaacttttt caggcatgcc
     2341 ggcagacgaa gccagtctgg ttcctgaagc gccggattcg atgaatgtca aatatttcct
     2401 ggacccgctg accacattct ctttcaagaa taccatcgtt tatatcctcg aaaaatcgct
     2461 gtcgtcgctg atttcacaaa ctcttaccac aaattattac aaacactttg aaaacttcta
     2521 ccctattgaa aacaaaataa atttcgacag ttgccagatt atcggtatgc aggaaatcag
     2581 cgcatccagc acttataacg agcatgttgt tttcaactcc gtgcaacaca atgtgcctgc
     2641 ctggaccatg ttcgccatgt tttttatttt tctgccgctg gcgggaagca tcatcaaaga
     2701 gcgcgactac ggcacattta tcaggctgcg gtttatgccc ggcagttttt ccaccctcat
     2761 tgcaggaaaa gtatttttat ataccctggt cggctgtatt cagtttctca tgatgctgac
     2821 tgccggaagg tgcctgttgc cggccttagg actgcccgct ctgataatag gcaacagcta
     2881 tgcggccatt gcagcaacgg tagtggcggc ctcattggca gcaacgggtt ttggcgtcct
     2941 gataggaact tttttcccga ctcatcagca gatggttacg tttggctcca tttcagtaat
     3001 tattttagcc gccctggggg gaatatttgt tcccatttac atcatgtcga aaactatggc
     3061 aaaggtgagt aaatactcac cactggaatg ggggctaaac gccttcaacg aaatttttct
     3121 gcatcagggc acctttataa acatttatat tgatgcactg aaactgctgc tgttttcagc
     3181 ggtggcattt ttactttcat ttttatacta taaattcaaa aggatacatt aaaacaggct
     3241 tcttatcatg actaccctta ctgacgaaat aaaaaagaat atcatcgaag agctgaactt
     3301 aacgcacgag ctggacgaaa tcagccacga cacacccttg ttcagcaacc ggcacaccca
     3361 cctggattcg cttgatgcac tgcgaattat tgtgatgctt gaaaaaaaat atggtattaa
     3421 gataaaagac ctgtcgcagc gaaaaaaaat cctttattct cttgatgcta ttgcaaagtt
     3481 ggtggaagaa caccggcaac aatccagtca atgagccaca acctgaaaaa ttcatacaaa
     3541 tttttcaccc ttcgggcctc actcacaaaa aaatcagcgg atacacatct tctcgaacaa
     3601 cccgctgtat gcagggaagc aggctgtaag cccggccttg gtcgccagtt caaaatcatg
     3661 aaaatcaaat cccggggcca cagtgcatcc tatcaaagaa taggaatctt cacgggtaac
     3721 ctcagctgca aaccagcatc catggggtat taccagttgg aatacttcct tgttaagaac
     3781 atcggggccc agtagggttt ccgacaaaac tccaacctgg tcgatgatat aaacccgtat
     3841 gggtgagcct gtgtaaaaat gccatatttc gtcagactgt aagcggtgga atgcggatat
     3901 ctggccgctt ttcagcagaa aatagatgga tgttgcaaag cacctttcgg cctgataccg
     3961 ggttgaaaga aaatctgcgc tgattttttc ctccgaacga tacacttctt taaagaatcc
     4021 tccttcgggg tgaggctgca gttgaagttg cgctatccag aaatcagccg tatcgctcat
     4081 cagcagttta attatttttt gtttgagaat ttttcttttt gctttttttc ttgaaggtga
     4141 acctgtcggg cagatatttt tcattgaaat agtaaagcaa tctaatggta atcacgctat
     4201 tgtattgtcg gcgagaccag gtttcgaacg tgaccggatt tacataatcg cgtttacgca
     4261 cgggggctaa ggagtatgaa taacgtatgt tgaatttcag atgtttccag atacgaaaac
     4321 gcaaatcggc aataatgtta aaatcatttt tggttttata ggtaatggta tctggacgct
     4381 cccagggttt tgttacattc cattcttttt ctttgaaagt taccatgcgc ccccacgaca
     4441 cacccagccc aaaggtccat gtgttgcggt cttcataatg caccataagg ggtgctgtca
     4501 ggtaattcca cttcaatgaa taataaggca actggagcga atcaacggct gaaggcggta
     4561 atttttggta cgcccctttt tcatcgtata aaatttccag actcaacgaa aaacgtttag
     4621 gcagcggcaa aattcctccc acaccgacac aggcccccat ttttttgaaa ccatatactt
     4681 catcgccatc cacctggctc agattcattc cggctataac cacgcccttg atttccctgt
     4741 ccaccccggg ccgtgtctca ttagtctcct gcgccctgac aacagaaaag cccaacaaaa
     4801 aacacagaaa aaaactaacc ggcagattta ttttcataga gcacaaaagt tcgtgtacaa
     4861 aattaattgc ttttttttaa taaagtcatt caaatattga ataacggaat taggcaggtc
     4921 aaagtatgcg tgattgattt taaaaaggtt ttttctcttt attcatgggt aaaaatcaca
     4981 cacgctgacc aattgttttt ataaatacct gatatggcca ttgccatgga accgttaaat
     5041 acaaaggttt tgaagcaggt tgcaaggcaa tcactttctg taatgattcc acccctgtgc
     5101 cctcaacggt accatttgcc cgctgcgtgt caccaggttg gtgccggcat tgctatccgt
     5161 aatatgcccg ataatactga cttcggcaaa agtttttatc ttttcgtaat cattttgcgc
     5221 gatggtaaac agcagttcat aatcttctcc cccattaagc gctgcagtag tcgggtcaat
     5281 ctttacactg tcgcacatcg ttattgttga catatcaaca gggatgcgtt cttcgtagat
     5341 ggcacatcct ttgcctgaat catggcaaat atgcagaata tccgaagcca ggccgtccga
     5401 tatatcaatc atagaagtag gcatgacatc gttttcagca aaaagtttta ctatatcttt
     5461 acgggcttcg ggtttcagtt gccgttccag aatgtagtcg tacccttcga ggtcgggttg
     5521 catgccggga ttggctaaaa atccggcttt ttcccgctcc agcagcagca aaccggcata
     5581 tgcgctgccc aggtcgccac tgacacaaat caggtcgttt tcgttggctg tgttacgata
     5641 cactacctta tcggctgaaa caacaccaat gacggtaaga ctcaaaaaca ttccggaagt
     5701 actgcttgtg gtatcaccgc ccaccagatc tacgttgtat ttttcgcagg ccagtttaat
     5761 gcccgcatat aattcttct
//
LOCUS       DAOSFP010000125         5775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12270_length_5775_cov_3.423077, whole genome shotgun sequence.
ACCESSION   DAOSFP010000125 DAOSFP010000000
VERSION     DAOSFP010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12270_length_5775_cov_3.423077"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLI16_04930"
     CDS             <1..850
                     /locus_tag="PLI16_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOH83936.1"
                     /translation="TECNGRHCALDPYLGTLGAVAEAARNVACSGAKPIAITNCLNFG
                     NPYNPGVYWQFVHAIKGMSNACKKFGTPVTGGNVSFYNQSNIGGNVVPVFPTPVIGMI
                     GLLNDKDNMMTMDFKSKGDLIYLLGETRNDINSSQYLIHHHKVQLSPPPYFNLDREFV
                     TQYVLSELINSKMIKSAHDVAEGGLFIALFEASMHNDLGFDITTDCEIRPDAFLFGES
                     QGRIVVSVDEDDETDFIDFLVDTKAPFTLLGHVTKGDMRVDDVSYFHIKEAKSIYDNA
                     LENHLK"
     gene            908..2230
                     /locus_tag="PLI16_04935"
     CDS             908..2230
                     /locus_tag="PLI16_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOH83937.1"
                     /translation="MFEKLFKLQKYQSNIRTEVLAGITTFVAAMYIIIVNPAMVSDTG
                     MPFSAVLTGTVVLSAFCTIMMGIYANNPILIAPSMGLNAFFTYFVVAGMGVPVPVALG
                     ALFWAGVVFFILSIFNIRTKIVEAIPRQIRFAIAGGIGLFIALLGFVNAGFIVVKPPF
                     IGLGEMNSVTLIFVGGIILISALMVKNVKGAMMIGIIVITLCCIPVGRWFGDASAVNH
                     GLPTLVNWKGFYQAPDFSFFMQLDFLNSLKLAVWPVIFAVCFTDMFDSLSTFVGVAEA
                     ADLKDENGEPRNIKQSLIVDAVATGLAGIFGTTSGTAYIESASGVAQGGRTGLTAVVA
                     GLLFIPFMFFSPLLSVVPAVATAPVLVIVGVFMMKPVMNIGWDKLDDAIPCFLAMILI
                     PLTYSITQGIIWGLLTWTLIKLINGKYNELSITLYIIDVFAVLLLIFE"
     gene            2251..2886
                     /locus_tag="PLI16_04940"
     CDS             2251..2886
                     /locus_tag="PLI16_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOH83938.1"
                     /translation="MKTSSLFLNLLIFACCLMSSVSLWAQPGKKDIIKDNLLFRAAMN
                     KEFADSSTTPLTREGLKNFDGLSFFPVNAKYCVISKLTLTPGEQAFEMPRSKGNTGTY
                     RKYGVAEFMLNGTLCRLSVYQYMKFINDSLYKDDLFLPFKDLTNGRQTYGGGRFLELK
                     IPDGDQLIIDFNKAYNPLCCYGNPKYSCPIPPEENHLKIKILAGEKAYKGK"
     gene            complement(2965..4434)
                     /locus_tag="PLI16_04945"
     CDS             complement(2965..4434)
                     /locus_tag="PLI16_04945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH83939.1"
                     /translation="MSISFLNKKYEVIITAFLFFVLNFFLKILFVSANDIAMDEPFSI
                     YYAQMDLSSIFTMLKTENNPPLHFLLLHYWIKAFGISPLSVRFLSVLFSALTAPLIYF
                     IGQKFFSKQTGLIAGLIFTLSYFHMYFSHEARVYPIFVFLTALCLYYFLSVVNRPERW
                     KPYIGLLIVNVLLVYSHYFGFFVVATEFVCIFFAGNLSRIWKKMSLVFFLLMLAYIPN
                     ALILWQRFSISVGQGTWVRQPEFTELYGNLNRFMNSRMVMAVLLVVAIIYIVLLIRDK
                     QFSLKIKAFFRTPFSRILLIWFVFPYLAMFFLSFWAPMFLDRYIIFISISFYLLIALF
                     LATFTRYTYVKTAGTIVLLILMALNLNLAPDNNRRVKQLAGTVKELKAQHPKSIVIIS
                     PEYSHLEFTYHFNINYFKDYNNTKALLQKNNIFPLRNLSSADKIKIQNQEVIYVDCGT
                     EFAFGESSILADLNTSHTANYITSVFEIYRIYLFSPGKQ"
     gene            complement(4444..>5775)
                     /locus_tag="PLI16_04950"
     CDS             complement(4444..>5775)
                     /locus_tag="PLI16_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83940.1"
                     /translation="ILSNVSVSSGNNTIDIIRIDSLGIIIDNKIIGDGPVYWANDFIK
                     TSDGGFLICGFTNKYPANSYDMLLIKTDSSLNLEWEKTYGGTDWDIANAVRETFDNAY
                     LMVGKTYSFGPSNDNVYVVKTNFQGDTLWTRTFGGDSSDYATSVDILFDSTYLIGGAT
                     NSFGYGNYDGYVLNLGRNGDTIWTKTYGEDKEDIIYSIKQTPDSGFVFVGSTMSYNAI
                     EHENWLMRFDKNHQYWWRLPEFWDIGPGDDMVYDVFIDDSARYILTGYTTGVGMGGKE
                     VLMLVMGDGDVFLCSLTDGSANDETGNQVIQSADGGYVVVGSTQGIGPGLSNIYLLKT
                     GSNCYFTHTPEHILAINENTAALSEKSMYITPTLSGGKFDLIIKEPLFSEGFSLAVYD
                     IMGRLIVSKKVNNSASQTFPIDISAAADGMYFIEVSNKQSHSTFKVVKCAK"
BASE COUNT         1595 a         1226 c         1295 g         1659 t
ORIGIN      
        1 gacagagtgc aatggtagac actgtgcttt ggacccttat ctgggaaccc tgggagcagt
       61 tgctgaagct gccagaaatg tggcttgcag tggagccaaa ccgattgcca ttaccaattg
      121 cttgaatttt ggcaatcctt acaaccccgg ggtatattgg cagtttgttc atgccataaa
      181 gggaatgtcc aatgcgtgca aaaaattcgg cacacccgtg acgggtggca atgtgagttt
      241 ctacaaccag tcgaatatcg gcggcaatgt tgtgcctgta tttccaacgc cggtaattgg
      301 gatgataggt ttgcttaatg ataaggataa tatgatgacc atggatttca agagcaaagg
      361 tgacctgatt tatttgctgg gcgaaacccg caatgatatc aactcgtcgc aataccttat
      421 ccatcatcat aaagtgcaac tttcgcctcc accatacttt aacctcgaca gggaatttgt
      481 tacccagtat gtgttgtcgg aactgattaa cagcaagatg ataaaatcgg ctcacgatgt
      541 tgctgaaggc ggcttgttta tagccttatt tgaagcttcc atgcataatg acctggggtt
      601 tgatattacc actgattgtg aaatacgccc ggatgccttc ctctttggtg aatcgcaggg
      661 gcgtatcgtt gtgagtgtgg acgaagacga tgaaacagat ttcattgatt ttctcgttga
      721 tacaaaagcc ccatttactt tattaggaca tgtaaccaaa ggcgatatgc gcgtggacga
      781 cgtttcttat tttcatataa aagaagcaaa atccatttat gataatgctc ttgaaaatca
      841 cctgaaatag aatccggttc tttttgggtg ttttttttcg ttataaagaa taataaataa
      901 acatcttatg tttgaaaaac tctttaaact tcaaaaatac caaagtaaca tcagaaccga
      961 agtattggcc ggcatcacca cttttgttgc cgccatgtac atcatcatag tcaatccggc
     1021 catggtgtcg gataccggaa tgcctttcag tgcagtgctc acagggacgg tggtgctaag
     1081 tgctttttgt accatcatga tgggaatata tgccaataac ccgatactga ttgcccccag
     1141 tatgggactg aatgcttttt ttacctattt tgtagtagca ggcatgggag ttcctgtccc
     1201 tgtggctctt ggagcattgt tctgggccgg tgtcgtgttt tttattttga gtatttttaa
     1261 tattcgtaca aaaattgtgg aggctattcc caggcagatt cgttttgcta tcgccggcgg
     1321 cataggactg tttattgcgc tgctcggttt tgtaaatgcc ggctttattg tcgttaaacc
     1381 gccgtttatc gggctggggg agatgaacag cgtcactttg atttttgttg gcggcataat
     1441 cctgatttca gcgctcatgg tcaaaaatgt gaaaggagcc atgatgatag gcatcatcgt
     1501 cattaccttg tgctgtattc ctgtggggcg atggtttggc gatgcttcgg ccgtgaacca
     1561 tggcttgcca accctggtca actggaaagg gttttatcag gcgcctgatt tcagtttttt
     1621 catgcagctc gatttcctga attctctaaa actggcggtc tggcctgtta tatttgcggt
     1681 ttgcttcacg gatatgttcg acagtctttc cacttttgta ggtgttgccg aagcggccga
     1741 ccttaaagat gaaaacggcg aaccccgcaa tatcaaacaa tcgcttattg ttgatgcggt
     1801 agccaccggc ctggccggta tttttggtac cacttcggga acagcctata tcgagtcggc
     1861 ctcgggtgtg gcacagggag ggcgcaccgg actaaccgcc gtagttgccg gattgctgtt
     1921 tatccccttt atgttttttt cacccctgtt gtccgtggtg cccgcggtgg ctactgcgcc
     1981 ggtgctggtt attgtgggcg tattcatgat gaaaccggtg atgaacattg gctgggataa
     2041 gctcgacgat gccataccgt gcttcctggc catgatttta atacccctga cgtattccat
     2101 tacccaggga atcatctggg ggctgctgac atggacattg atcaagttga ttaatggtaa
     2161 gtacaacgaa ctcagtataa ccctgtacat aattgatgta tttgccgtgc tcttgctgat
     2221 ttttgaataa aatttctgta tgtcctcatc atgaaaacaa gctcactatt tttaaatcta
     2281 ttgatttttg cgtgttgtct tatgagttct gtgagcttgt gggcacagcc cggcaagaag
     2341 gatattataa aagataattt gcttttccgg gccgcgatga ataaggagtt tgctgattcg
     2401 tccaccactc ccttaacccg tgaaggctta aaaaacttcg atggcttgag ttttttcccg
     2461 gtcaatgcaa agtattgcgt aatttcaaaa cttaccctca cacccggaga acaagctttt
     2521 gaaatgcccc gttccaaagg caatacaggc acataccgta agtatggagt ggcggagttt
     2581 atgctcaacg gaacgctgtg ccgcctgagt gtgtatcagt atatgaaatt tataaatgac
     2641 tctttataca aagacgattt gtttttgcct ttcaaagacc ttaccaatgg caggcaaacc
     2701 tacggcgggg gccgctttct cgagctgaaa attcctgatg gagaccaact gatcatcgat
     2761 ttcaacaagg cctataatcc tctctgctgt tatggaaatc caaaatattc ctgtccgata
     2821 ccgcccgaag aaaatcattt gaaaatcaaa atccttgccg gcgaaaaagc gtacaagggt
     2881 aaatgaaaat ttccagcgaa ttttttgtgg aaaaaattga cagaccggca cgtactgcat
     2941 gaatatcggc ggcaatagca gccattactg tttcccggga ctgaacagat atatgcggta
     3001 aatctcaaat accgaagtga tgtagtttgc tgtatggctt gtattcaggt ctgcgagtat
     3061 ggagctctcg ccaaaagcaa attcagtgcc gcaatccacg tatatcactt cctggttttg
     3121 tatttttatt ttgtccgcgg aagaaagatt acgcagagga aaaatattgt ttttctgcaa
     3181 aagggctttg gtgttattgt aatctttgaa gtagttgata ttgaagtgat aagtaaattc
     3241 caagtggctg tattccggcg atatgatgac aatagatttc gggtgctgcg cctttagttc
     3301 tttcacagtg ccggcaagct gtttcaccct ccgattgttg tcgggtgcca gattgagatt
     3361 taaagccatt aatatcagca atacaattgt gcctgctgtt tttacatatg tgtaccgggt
     3421 gaatgtagct aaaaacaatg ctatcagcaa ataaaaactg atagaaataa aaatgatata
     3481 tctgtcgaga aacatgggcg cccaaaaaga cagaaaaaac atagccaggt acggaaatac
     3541 aaaccaaatc aataaaatcc gggagaaagg ggtgcgaaaa aatgctttta tcttaagcga
     3601 aaactgttta tcgcgtataa gtagcactat gtagataatt gcaacaacca gcaaaacagc
     3661 catcaccatc cttgaattca taaaacggtt caggttgccg tataattccg taaactccgg
     3721 ttgccttacc catgtaccct gcccaacgga tatgctgaat cgttgccata aaatcagcgc
     3781 attgggaata tatgccagca tcagtaaaaa aaacaccagc gacatttttt tccatatgcg
     3841 gctgaggttg ccggcaaaga aaatgcaaac aaattcggtg gccacaacaa aaaaaccgaa
     3901 atagtgcgag taaacaagca aaacattcac tatcagcagc ccgatatagg gtttccacct
     3961 ttcgggccgg ttaaccaccg aaagaaaata ataaagacac agcgccgtaa gaaaaacaaa
     4021 aataggatac accctggcct catgcgaaaa atacatgtgg aaataagaaa gcgtgaaaat
     4081 gagacctgca atcagtccgg tttgtttcga aaagaatttt tgcccgataa agtaaatcag
     4141 cggagctgtg agtgcgctga acagtaccga taagaacctc accgacagag gactgatgcc
     4201 aaatgctttt atccagtagt gtaacaataa aaaatgcagc ggaggattgt tttctgtttt
     4261 cagcatagtg aatatcgacg acaggtccat ttgggcgtaa taaatgctga aaggctcgtc
     4321 catagcgatg tcgttggccg aaacaaacag tattttcagg aaaaaattca gaacaaaaaa
     4381 caggaatgct gtgatgatca cttcatattt tttatttaag aatgatatgc tcatactttc
     4441 cggttatttt gcgcacttta caactttaaa agtgctgtgg gattgtttgt tcgaaacctc
     4501 aataaaatac atgccatccg ctgcggctga aatatctatt gggaaagtct gcgaagcgct
     4561 gttattaact tttttggaaa ctataagtcg gcccatgata tcataaaccg caaggctgaa
     4621 accttcggaa aacaatggtt ctttgatgat taagtcaaat tttccgcccg acagtgttgg
     4681 cgtaatgtac attgattttt ctgacagggc tgcagtattt tcatttattg caaggatatg
     4741 ctcgggcgtg tgtgtgaaat agcaattgct gccggttttc agcaggtata tgttcgagag
     4801 gccgggccct ataccctggg tgctgcccac aacgacatag ccaccgtcag cagattgaat
     4861 tacctggttt cctgtttcat cattagcaga gccatccgtg aggctgcaaa gaaaaacatc
     4921 accatcaccc attacgagca ttagcacctc cttgccaccc atgcctactc cggtggtgta
     4981 tccggttagt atgtaccgcg ccgaatcatc gataaacaca tcgtatacca tatcatcccc
     5041 cgggccaata tcccaaaatt caggtagcct ccaccaatat tgatggtttt tgtcaaacct
     5101 catcagccag ttttcatgct ctatggcatt ataactcatg gtagaaccca caaaaacaaa
     5161 gcccgaatca ggagtttgct ttatgctgta aatgatgtct tccttgtctt ctccgtaagt
     5221 ttttgtccag atggtgtcgc cgttcctgcc caggttcagg acatatccgt cgtaattgcc
     5281 atatccgaaa ctattggtag cgcctcctat caggtaagtg ctgtcaaaaa gaatatccac
     5341 cgaggtggca taatctgagc tgtcgcctcc gaatgttctg gtccacaggg tgtctccctg
     5401 aaaattggtt tttacaacat agacattatc gttcgacgga ccaaagctgt atgttttgcc
     5461 aaccataagg taggcgttgt cgaaggtttc tcttactgcg ttagcaatat cccagtcggt
     5521 gccaccgtat gttttctccc attccaggtt gaggctggag tcagttttga tgagcaacat
     5581 atcgtagctg ttggccgggt atttattagt aaatccacag ataagaaatc ccccgtcaga
     5641 ggtttttata aagtcgttgg cccagtaaac cggaccgtca ccgattattt tgttgtctat
     5701 aataataccg agagagtcga ttctgattat gtcgatagta ttattgcccg acgaaacact
     5761 tacattgctt aaaat
//
LOCUS       DAOSFP010000126         5764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12297_length_5764_cov_3.176563, whole genome shotgun sequence.
ACCESSION   DAOSFP010000126 DAOSFP010000000
VERSION     DAOSFP010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12297_length_5764_cov_3.176563"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1323)
                     /gene="porV"
                     /locus_tag="PLI16_04955"
     CDS             complement(85..1323)
                     /gene="porV"
                     /locus_tag="PLI16_04955"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOH83941.1"
                     /translation="MKRNLLIFVSFILLGTMVFAQGSQTNDLLKNKLNTITTAVPFLL
                     IAPDARGGGLGDAGAASSPDANSMHWNPAKYGFIDKDFGFSVSYSPWLHKLVNDINLA
                     YLGAYKRINKRQVVSMSLRYFSLGNITFTNETGQNIGQYKPNEFSLDASYCFKFTNEV
                     SGAIALRYIYSNLTGGIYVAGAQSKAGQAVAADISVFYEKPIDIKGMETKFTLGANIS
                     NIGNKISYTENLDRDFIPTNLRLGFGYEMNFDKNNSLALLLDFNKLLVPTPPIYQLDS
                     TGKYVYDVDGNKIIEKGKDPNVGVVRGMVQSFYDAPDGFKEEMKEINIAVGLEYWYAK
                     QFSIRAGYFNEASTKGNRKYVTLGAGLRYSIFGLDFAYLIPVTQRNPLENTLRFSLLF
                     NFDKVKAKKEKEGEEIKKNE"
     gene            complement(1419..4856)
                     /gene="porU"
                     /locus_tag="PLI16_04960"
     CDS             complement(1419..4856)
                     /gene="porU"
                     /locus_tag="PLI16_04960"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOH83942.1"
                     /translation="MKFRFTLICFLVCVVSVSLAQKSGSGNFADNSILSSGQWYKIGT
                     INQGVHCLTYADIVQMGVSGPLSSADFRLFGNGGAMLPESNAAFRYDDLAENPVMVDD
                     GGDGYLNEGDRILFYGQNTVSWSYNAAFQRFDRTINLYADTVYHFFTFDHGPGKRIQI
                     YPQISQPADTSVSTFDEHLYHERDSVNLLRSGKQWYGEVFNDLETKTFKFLMTDVSPS
                     DPLSIRINMAVSSIQKSVVTSAVDGVVHSSDTILPIDGNVNSDYAKPKTISLSVFPQE
                     DTVVVSISYNKPQTSSVAWLNCIDLSGVRNLIYRGGQLNFRNVATSGQAVSRFDVTTT
                     AVIPEIWNITDFMEISAMPVAVNGTVCSVTAYTDSLLEFVAFDDSDLLHPILLGATAN
                     QNLHGLSQAELVAVTHPDFLNQANTLADFHRATDGMMVAVATTEQIYNEFSSGKPDPA
                     AIRDFLRMFYERGQSDSVHRIKYVLLMGDGSYDMKNRLKANTNFVPSWQTNNSLVPTS
                     SYVSDDFFTMLDPQEGENLVGNLDIGAGRFPVTTSQEAAVVVEKCIRYGTREDFIPDS
                     YETGTVSNFDPWRNVITFIADDEDINLHLKQAEKLVTLVDSITKNYNINKIYLDAYKQ
                     INTPFGTKYPEVNEAIDKQVAKGTLMINYTGHGGETGLASENVLTMDEIEQYTNYYNL
                     PIFVTATCEFSRYDNPAFLSAGEKLLLSQKGGSIALFSTTRVAYAHSNEIVNRNLLKT
                     AFIPVSGDRVRFGDMIRKAKNLCAAGVYMQNFTLLGDPALAFAVPEYQVITDFCGIDT
                     ASANDTLFNNSVVTVRGHIADADSVWQPDFNGKLFPLVFDKPVLCQTLANDPGASQVR
                     DFYIQQNEIYRGNVTVTNGTFEFSFFVPRDISFSDGFGKISYYAKSLNHDAVGVLDSL
                     KLFNDGNNESDDITGPAIDAFMETLSFVDGDKTSSQPLMLLHLWDTSGINSYNIGIGH
                     EITAVLDDDFNNPICLNDYFLQEADNYAAGSITYRFNTLAYGAHKIIVKAFDLLNNSS
                     ETEIHFYVEPPADAGFGKVYNYPDPFYDYTTFYIEHNMSLAEMQVKIHVFDITGRLSA
                     EIDFEIPAGQYVPAKWRWNGCNQHGEPLSKGLYSYTVTLTDNNGKQKRCSDKLIILK"
     gene            complement(4916..>5764)
                     /locus_tag="PLI16_04965"
     CDS             complement(4916..>5764)
                     /locus_tag="PLI16_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83943.1"
                     /translation="IIYKGKTRVTNGDFKFTFIVPKDIAYNFGNGRISYYSQNGTTDA
                     NGFYEDFIIGGTSNDSITDGQGPELKIFMNDVRFVSGGITDKNPVLLLFLNDSAGVNT
                     IGNGIGHDIAAVLDENTDKTFVLNDYYESDMDTYQSGVVRYPFSNLENGEHKLRVKAW
                     DVYNNSAESEIDFVVAESAELALNHVLNYPNPFTTYTEFWFEHNQPCCALDVQIQIFT
                     ITGKIIKSIQTTVETNGYRADPIPWDGRDDFGDVIGKGVYVYMLKVKNSTGQISQKTE
                     KLVILK"
BASE COUNT         1589 a         1286 c         1265 g         1624 t
ORIGIN      
        1 cctttttcag aagggatatt tattccgccg agaaataaag cagcgccggt ttcgagttta
       61 tgtgtgtcga atccaaaccc tatcctactc attctttttt atttcttcgc cttccttttc
      121 tttcttggct tttaccttgt cgaaattgaa taacaggctg aaccggaggg tgttttcgag
      181 ggggttgcgt tgtgtaacag gaatcaggta agcaaagtca aggccgaaaa tactgtaacg
      241 caggccggcg ccgagggtaa catacttacg gtttcctttg gtagatgctt cgttaaagta
      301 tcctgcacgg atggagaatt gttttgcata ccaatattcg aggccgacgg caatatttat
      361 ttctttcatt tcttccttga atccgtcagg agcatcataa aaagactgta ccatgccgcg
      421 cactacaccc acattcgggt ctttcccttt ttcgatgatt ttgttaccat ccacatcata
      481 aacgtatttt ccggtagaat cgagctgata tatcggcgga gtaggcacaa gcagcttgtt
      541 aaagtcgagc aataatgcca gggaattgtt tttatcaaaa ttcatctcgt aaccaaaacc
      601 aagccggagg ttcgtgggaa taaagtcgcg gtcaagattt tctgtataag aaattttgtt
      661 tccgatattt gatatgttgg cacccagggt gaatttggtc tccatacctt ttatatcgat
      721 aggtttttcg taaaacacgc tgatgtcggc agccacggcc tgtccggctt tcgactgtgc
      781 ccctgccaca taaatccccc ccgtgaggtt ggaatagata tagcgcaagg caatggcacc
      841 ggatacttcg ttggtaaatt taaaacagta ggaagcgtcg agtgaaaatt cgttgggttt
      901 atactgtccg atgttttgtc cggtttcgtt ggtaaacgta atatttccca acgagaaata
      961 acgcagcgac atagaaacca cctgcctttt gttaatacgt ttgtaagctc ccagataagc
     1021 aagatttata tcatttacca gcttgtgcaa ccaggggctg taagaaacag agaaaccaaa
     1081 atctttgtca atgaaaccat acttggccgg attccaatgc attgaattgg catcgggcga
     1141 ggatgcagcg ccggcatcac ccagtccgcc gccacgggca tcgggggcta taagcaggaa
     1201 aggcacggcc gtggttatgg tatttaattt gtttttcagc aaatcatttg tttggctacc
     1261 ttgagcaaat accatagtcc caagcaaaat gaacgataca aagattaaaa ggtttctttt
     1321 cattaacaat tggaattttc gtgagtgcaa aaataacgat aattttttta ctatattgta
     1381 ctgttaacat ttttgtatta cgcagcttcc taaatttctt attttaaaat aatcaatttg
     1441 tcggagcacc gtttttgctt gccattgtta tcagtaaggg ttaccgtata ggaatacaaa
     1501 ccttttgaca acggttctcc atgttgattg catccattcc agcgccattt ggcaggcacg
     1561 tattggcctg ccggaatttc aaaatcaatt tctgccgaca accttccggt aatatcaaaa
     1621 acgtgtattt ttacctgcat ttcggccagg ctcatgttat gctcaatata aaaggtggtg
     1681 taatcgtaaa atggatccgg ataattgtac accttaccaa aaccagcatc agcaggaggt
     1741 tcaacgtaga aatgtatttc ggtttccgaa gagttgttta gcaaatcaaa agctttgaca
     1801 atgattttgt gcgcgccgta ggccagggtg ttaaaacggt acgtaatact tccggcggca
     1861 taattatcgg cttcctgtaa aaaataatcg ttgaggcaaa taggattatt gaagtcatcg
     1921 tcgagaaccg cagttatttc atggcctatt ccgatattat aactattgat gccactggtg
     1981 tcccacaggt gcaaaagcat cagcggctga ctggaagtct tatccccgtc aacaaacgac
     2041 agggtttcca taaaggcatc tattgccgga ccagtgatat catcgctttc attatttcca
     2101 tcattaaata atttgaggct gtccaaaacg cccacggcat catgattaag gcttttagcg
     2161 taatagctta tttttccgaa accatccgaa aaacttatgt cgcggggaac aaaaaaagag
     2221 aactcgaagg tgccgttggt taccgtcacg ttgccgcggt aaatttcgtt ttgttgtata
     2281 taaaaatccc tgacctggct tgccccgggg tcgttggcca gagtttggca taagaccggt
     2341 ttatcaaaca ccaaaggaaa aagtttcccg ttaaaatcag gctgccatac tgaatccgcg
     2401 tcggcaatat gccctctgac agttaccaca ctgttgttaa acaacgtatc gttggcggaa
     2461 gcggtatcta tgccgcaaaa atcggtgatt acctgatatt caggaacagc aaaggccaga
     2521 gccgggtcgc caagcaaggt gaaattctgc atataaacac cggcagcaca caagtttttt
     2581 gctttacgaa tcatatcacc aaaccgcacc ctatctccgg agacaggaat aaaagcggtt
     2641 ttcagcaaat tacggttcac aatttcgttg ctatgggcat atgccacacg cgtggtcgaa
     2701 aacaaagcga tgctgccgcc tttttggctg agcaacaatt tttcgcccgc cgacaggaag
     2761 gcaggattgt cgtaccggct aaactcacac gtggcagtaa caaaaatcgg taaattataa
     2821 taattggtgt attgttcaat ctcgtccata gtcagcacat tttcggaagc cagccctgtt
     2881 tctccgccat ggccggtgta gtttatcatc agggttcctt tggccacctg tttatcaatg
     2941 gcctcattta cttcgggata ttttgttcca aaaggggtgt ttatttgctt gtaggcatcc
     3001 agataaattt tattgatatt gtagtttttt gttatagaat ccactaaagt taccagtttc
     3061 tcggcctgtt tcagatgcag gttaatatcc tcatcatctg caataaaagt tatcacatta
     3121 cgccagggat caaaattcga taccgtgccg gtttcgtatg agtcgggaat aaaatcttct
     3181 cgggtaccat agcgtatgca tttttccaca accacggcgg cttcctggct tgtagtaacc
     3241 ggaaatcgtc cggcaccgat atcgaggttg cccaccaggt tttcaccttc ctgtggatcg
     3301 agcatagtaa aaaaatcatc ggaaacatag gaggaagttg gtaccagcga gttattggtt
     3361 tgccatgaag ggacaaaatt ggtattggct ttcaggcggt ttttcatgtc gtaagagcca
     3421 tcgcccatca gcagtacgta ttttatcctg tgcacggaat cgctttgtcc gcgttcataa
     3481 aacatgcgca ggaaatcgcg gattgccgcg ggatcaggtt tgcccgacga aaactcgtta
     3541 tatatctgct ccgtagtagc cactgcaacc atcatcccgt ctgtagccct gtgaaaatcg
     3601 gccagggtgt tggcttgatt taaaaaatca ggatgcgtaa ccgccaccaa ttcggcctgt
     3661 gacaggccgt gaaggttctg attggccgtg gctcccaaca gtatgggatg taacagatca
     3721 ctatcgtcaa aagcaacaaa ttcaagcagg ctgtccgtat aggcggtcac agaacagaca
     3781 gtaccattta cagcgaccgg catggcggaa atttccataa aatcagtaat attccagatt
     3841 tcgggaataa cagccgtggt ggttacgtca aaacggctaa ctgcctgccc gctggttgcc
     3901 acattgcgaa aattcaattg gccgccccgg tatatcaggt ttctgacacc cgacaaatcg
     3961 atgcaattca gccaggccac agaggaagtt tgcggcttgt tatatgaaat acttactaca
     4021 acggtatcct cctgaggaaa aacactgaga cttattgttt tgggtttggc ataatcggaa
     4081 ttaacattac cgtcaatagg caatatagta tcggaagaat gtaccacccc atcaacagcc
     4141 gaggtaacga cacttttttg tattgaactg acagccatgt ttatgcggat actgagcgga
     4201 tcggaaggcg aaacatcggt cataagaaat ttgaatgtct tggtttccaa atcattaaat
     4261 acttctccat accattgttt ccctgaacgc aacagattta ccgagtcccg ctcgtgatag
     4321 aggtgttcgt cgaaagtgct gacactggta tcagccggtt gtgaaatttg cgggtagatt
     4381 tgtattcttt tgccggggcc atgatcaaaa gtaaaaaaat gatacaccgt gtcggcatac
     4441 aggtttatgg tccggtcgaa acgctggaaa gcggcattgt aactccaact gacagtattt
     4501 tgtccgtaaa agagtatccg gtctccctcg ttgaggtagc cgtcgccacc gtcatcaacc
     4561 atcacaggat tttcggccag gtcgtcgtac ctgaaagctg cattgctttc ggggagcata
     4621 gcgccgccat tgccaaacag cctgaaatca gccgaactga gtggcccgga aacgcccatc
     4681 tgcactatat ccgcataagt aagacagtgt acgccctgat ttattgtgcc aatcttatac
     4741 cactgtcctg acgataatat tgaattatct gcgaaattcc cgctgccgga tttttgagca
     4801 agggaaacgc ttacaacaca aacaaggaaa cagataagtg taaaccggaa tttcattttg
     4861 cagataatga acaaaattat aaaaaaaatg gcaataatat ggctattgcg ccggtttatt
     4921 tcagaatcac cagtttttca gtcttttgtg agatttgtcc tgtgctgttt tttactttca
     4981 gcatataaac atatactcct ttaccaatca catcgccgaa atcgtccctg ccgtcccagg
     5041 ggatggggtc ggcccggtag ccattggttt caacagttgt ctgaatactt ttgataatct
     5101 ttccggtaat ggtaaaaatc tgaatttgca catccagcgc acagcagggc tgattgtgtt
     5161 cgaaccagaa ttcggtatag gtggtaaaag ggttgggata attcaacaca tggttaagag
     5221 ccagttcggc cgattcggcg accacaaaat caatttccga ctcagcggaa ttattataaa
     5281 cgtcccatgc tttcactctc agcttatgct ctccgttttc aagatttgaa aagggataac
     5341 ggactacccc gctctgatag gtatccatgt ccgactcata ataatcgtta aggacaaagg
     5401 ttttgtcggt attttcgtcg agaacagcgg caatatcatg tcctatgccg ttgccgatgg
     5461 tgtttacacc tgccgaatcg ttcagaaaca acagcaacac aggattttta tctgtaatgc
     5521 cgcccgacac aaaacgaaca tcattcataa agatcttcag ttcgggacct tgcccatcgg
     5581 tgatgctgtc attgctggtt ccaccgataa taaaatcttc atagaacccg ttggcatccg
     5641 tggtgccatt ctgcgaataa tagctgatac ggccgttacc aaaattatac gctatgtcct
     5701 tgggaacgat aaacgtaaac ttaaaatcac cgttagttac ccgcgtcttt cctttataaa
     5761 tgat
//
LOCUS       DAOSFP010000127         5743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12362_length_5743_cov_4.346695, whole genome shotgun sequence.
ACCESSION   DAOSFP010000127 DAOSFP010000000
VERSION     DAOSFP010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12362_length_5743_cov_4.346695"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLI16_04970"
     CDS             <1..482
                     /locus_tag="PLI16_04970"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOH83944.1"
                     /translation="ELFGLDLDRDAIANMASELGSDCTFFVDNRPCFVSGTGADIAPV
                     ELSLKGYYVMVVKPAVSVSTVEAYRMVTPQKPVLRLSDVIKMPVTAWEKCLSNDFESP
                     VMEQYPTIRRIKDRLYEMGAVYAAMSGSGAAVYGLFEQEVDTDKDFAGCQLWKGWLY"
     gene            complement(483..1559)
                     /locus_tag="PLI16_04975"
     CDS             complement(483..1559)
                     /locus_tag="PLI16_04975"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH83945.1"
                     /translation="MNKIDIYIIKKFLGTFFLSISLIIIIVIIFDISENIDDLISSEA
                     PLNEIIFNYYLNFIPYFINLFSPLFTFIAVIYFTSKMATHSEIISILSNGISFGRMLK
                     PYMITAALLTALSFYLANFLIPYTNINLWDFKYTYIKNKYYNDSRNIHMQIAPGAFVY
                     VESYDVDDNKGTRFTMEHFKDGVLYYKMNANYIQWDSIKAKWNINNYSIHLMNGMQEK
                     IITGAQKDTAINLTPEDFHRKQDNMDVMNFSQLRKFIKDEKLKGSDNIRFYEVEKHKR
                     IAFPFATFILTLIAVSVSSRKVRGGVGLHIAFGMALTFAYILFMRITETFATYGNLSP
                     WMAAWLPNIIFVFVAAYMIKVAPK"
     gene            complement(1689..3821)
                     /locus_tag="PLI16_04980"
     CDS             complement(1689..3821)
                     /locus_tag="PLI16_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOH83946.1"
                     /translation="MKVYQTNEIKNIALIGGSKAGKTTLAECMLFEGGVIKRRGSVED
                     KNTISDYRDIELERQNSVFSTVMFTEYADKKINIIDNPGFDDFIGEVFASLKVCETAV
                     MVVNAQNGVEVGTEITWRHTSRLHVPVLFVVNQLEHEKANFDENIRQLKNQFGPKVSV
                     VQYPVSTGIGFDSIIDVVKMKMYKYNKEGKAEIMDIPASEMDKAQDLHATLIEAAAES
                     DEALMEVFFDKGTLTEEEFYKGLKLGIKNRNLFPVLCTSSKMNIGVARLLEFIANSTP
                     SPDEVPAPKNIEGKEIPCTASGPAAAFVFKTSIETHIGEVSYFKILSGEITEAIDMIN
                     VNNGTKERVSQVYICAGKNREKIAKAVAGDIAATIKLKSTFTNNTLVLTKNSDQEMAP
                     IEFPNPKYRVAVKAINTADDEKLGGILNDMKRIDPTLIPEYSKELRQLIVHGQGELHL
                     TVAKWQIEKINKIDIEYLPPRIPYRETITKQAKAVYRHKKQSGGAGQFGEVHMLIQPF
                     TDTMVDPTEFPIRGREDHELSWGGKLIFHNCIVGGSIDARFMPAILKGVMEKMEEGPL
                     TGSYARDIAVYIYDGKMHPVDSNEISFKLAGRNAFKEAFKNAGPKILEPIYDVEVIVP
                     EDKMGDVMTDLQGRRAVIMGMDSEGNYQKILAKVPLAEMNRYSTALSSITSGRATYGM
                     KFAEYTQVPGDLQTQLLKAYEEQEKDEE"
     gene            complement(4039..5019)
                     /locus_tag="PLI16_04985"
     CDS             complement(4039..5019)
                     /locus_tag="PLI16_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOH83947.1"
                     /translation="MKKILGIGNALVDIMTILDNEEFLQQINIPKGSMQLVDIDTSAS
                     ILKRSEKFKTELSSGGSAANTIHGLANLGATTSFIGKTGNDKYGEFFANDMRNTGINP
                     ILLTGSSDTGCAIALISPDSERTFATYLGAAIELSQEDLAADMFSEFDYIYIEGYLLQ
                     NHALVEKILEYADQHHCKICLDLASYNVVEANLDFLKIILAEYVDIVFANEEEARAFT
                     GTDPEKALDKLGEICGIAVVKVGANGSLVRQGKNTFAIQAIQSNCIDTTGAGDAYAAG
                     FLYGLANDLPLDKCGAIGSLLAGKVIEVVGAKMKENTWAEIREKIPHITK"
BASE COUNT         1471 a         1176 c         1361 g         1735 t
ORIGIN      
        1 atgaattatt tggccttgat ctggaccggg acgcaatagc aaacatggca tcggaactgg
       61 gcagcgattg cacttttttt gttgacaacc ggccatgttt tgtttccggt accggtgccg
      121 atatcgcccc cgtggaactt tcgctgaaag ggtactacgt gatggttgtc aagcctgcgg
      181 tttccgtttc aacggtggag gcataccgca tggtgacacc gcaaaaacct gttttgcgcc
      241 tttcagatgt gataaaaatg cctgttactg cctgggaaaa atgcttatcc aatgattttg
      301 aaagcccggt aatggaacaa tatccgacaa tacgccggat aaaagaccgg ctctatgaaa
      361 tgggcgccgt ttatgcggcc atgtcgggca gcggagcggc tgtttacggc ttgttcgaac
      421 aggaagttga tacggacaaa gattttgccg gatgccagtt gtggaaaggg tggctatact
      481 gattatttgg gcgccacttt tatcatgtag gcagccacga atacaaaaat aatgttgggc
      541 agccaggcgg ccatccacgg ggaaaggttg ccataggtgg caaaggtttc agtgatgcgc
      601 ataaacaata tataggcaaa ggttaatgcc atgccaaagg caatatgcag tcccacgccg
      661 ccccggactt tacggctgga taccgataca gctatcagcg tgaggataaa cgtggcaaac
      721 ggaaatgcaa tacgtttgtg cttttcaact tcataaaacc tgatattgtc cgaacctttg
      781 agcttctcat ctttgatgaa cttacgcaac tgcgaaaaat tcataacatc catattgtct
      841 tgttttctgt gaaagtcttc gggggtaaga ttaatggcgg tgtctttttg tgccccggtg
      901 ataatttttt cctgcatccc gttcatcaga tgaatactgt agttgttgat gttccatttt
      961 gccttgatgc tatcccactg aatgtagttt gcattcattt tataatacaa cacgccatct
     1021 ttaaaatgct ccatggtaaa tcgggtacct ttattatcat ccacatcata gctctccaca
     1081 taaacaaagg cgccgggggc aatttgcata tggatatttc ttgagtcgtt gtaatatttg
     1141 tttttgatgt aggtatattt aaaatcccac aaattgatgt tggtgtatgg gatgaggaaa
     1201 ttggccaggt aaaaagacaa ggcggtcagc agggcggcgg taatcatgta tggtttcagc
     1261 attctgccaa aactgatgcc attgctcaga atggatatga tttccgaatg ggtagccatt
     1321 ttggaggtga aataaatgac cgcgataaag gtgaacagcg ggctgaaaag gttgataaaa
     1381 taagggatga aattaaggta ataattaaaa ataatttcgt tgagaggggc ctcgctggag
     1441 atcaggtcat caatgttttc ggagatgtca aaaattatta caattatgat tatcagcgaa
     1501 atagaaagaa aaaaggttcc taaaaacttt tttatgatat aaatatcaat tttgttcatt
     1561 tttttctttt tcgcctggtg ggtgctgata agttaccaac agaaattacg caaatttatg
     1621 caaattagtg tataaaaatt aaaaaaaaac ctgcccggag agagcaggtt tttgaaatga
     1681 aaaatgattt attcttcgtc tttttcttgt tcttcgtaag ccttgaggag ttgtgtctgc
     1741 aggtcgccag gtacctgggt gtattccgca aacttcattc cgtaagtagc cctgccgctg
     1801 gtaatcgaac tcagggcggt ggaatacctg ttcatttcgg ccaaaggcac tttggcgagg
     1861 attttctgat agtttccttc gctgtccata cccataataa cggcccttct tccctgcagg
     1921 tcagtcatca catcgcccat tttatcttcg ggaactatta cttccacatc ataaataggt
     1981 tccaggattt ttggaccggc atttttgaaa gcttccttga aggcgttacg accggccagt
     2041 ttgaaagata tttcgtttga atcaaccggg tgcattttgc catcataaat atacacggca
     2101 atatcgcgtg cataagaacc tgtgaggggg ccttcttcca ttttttccat tacccctttg
     2161 aggatggcag gcataaaacg tgcgtcaata gaaccaccga cgatacagtt gtgaaaaatc
     2221 agttttccac cccaggacag ttcatgatct tctcttccgc ggatggggaa ttcggtagga
     2281 tccaccatcg tgtcggtaaa gggctggata agcatgtgta cctcgccaaa ctggcctgcg
     2341 ccgcctgatt gttttttgtg gcgataaact gcttttgcct gtttggtgat ggtttcgcgg
     2401 taaggtattc ttggcggcag gtattcgatg tctattttat tgattttttc gatttgccat
     2461 tttgccacgg taagatgcaa ttcgccctgt ccgtgaacga taagttgtct taattccttg
     2521 gaatattccg gaatcagtgt cgggtcgatg cgtttcatat cgttgagaat gccgcccagt
     2581 ttttcatcat cggcagtgtt gatggctttc acggctacac ggtatttcgg gttgggaaat
     2641 tcgataggag ccatttcctg gtcggaattt tttgtgagca ccagggtgtt gttggtaaaa
     2701 gtgcttttta acttgatagt ggcggcaatg tcgccggcaa cggctttagc aattttttca
     2761 cggtttttgc cggcacagat atatacctgc gaaactcttt ctttggtgcc gttgttgaca
     2821 tttatcatat caatggcttc ggtaatctct ccggagagga ttttgaaata agatacttca
     2881 ccaatgtgtg tttcgatgga tgttttgaat acaaaagcag ctgcagggcc ggaagcggtg
     2941 cagggaattt cttttccttc gatgtttttg ggtgcgggta cttcgtcggg cgaaggtgtt
     3001 gagttggcaa taaattcgag caggcgggca acaccgatat tcattttaga ggaggtacaa
     3061 aggacaggga agaggttcct gtttttgatg ccgagtttca ggcccttgta aaattcttct
     3121 tctgtgaggg tacctttgtc gaaaaatact tccatcaagg cctcatcgct ctcagcggca
     3181 gcttcgataa gggtggcgtg cagatcctgg gctttgtcca tttcggaggc agggatatcc
     3241 attatttcgg ccttgccttc tttgttgtat ttatacatct tcatctttac cacatcaatg
     3301 atggaatcga atccgatgcc tgtgctgaca ggatattgaa ccacagatac tttaggtcca
     3361 aactggtttt tcagttgacg gatgttttcg tcgaagttgg ctttttcgtg ttccagttga
     3421 ttaactacaa ataataccgg tacatgcagg cgcgaggtgt gtctccaggt tatttcggta
     3481 cctacttcca cgccgttttg ggcattgaca accatgacag cggtttcgca aactttcagg
     3541 gaggcaaaaa cttcgccaat aaaatcgtcg aagccggggt tgtcgataat atttattttt
     3601 ttgtcggcat attcagtaaa cattaccgtc gagaacactg aattttggcg ttcaagttct
     3661 atatcacgat agtctgaaat ggtgtttttg tcttccacag agcctcttct tttgataact
     3721 ccgccttcaa aaagcataca ctcggccagg gtggtttttc cggcttttga tccgccgatc
     3781 aatgcaatgt ttttaatttc gtttgtctga tataccttca tttttgtaat taattattaa
     3841 tggattaaac tgttttttgc aataaggtgt gcaaaggtat aaaatattat gaaaaaaaat
     3901 agtaccgtga agaatttatc tgatataaaa cttcctgata gaataaaacc caaaacaagc
     3961 aatcggagtg ttcgacctgt tgcttgattt gggttggtcc gggataaaat atggagtaat
     4021 gaaaaatgtg ttttttacct atttagtgat gtgggggatt ttttccctga tttcggccca
     4081 ggtattttct ttcatttttg cgccgaccac ttctattact tttcctgcca gcagcgaacc
     4141 aatggcgccg catttgtcga gaggaaggtc gttggccagg ccataaagaa atcctgcagc
     4201 ataagcatcg ccggcgccgg tggtgtcaat acaattcgat tggatagcct gaatggcgaa
     4261 ggtgtttttg ccctgtctta ccaaagagcc attggccccg actttgacaa cagcaatacc
     4321 acaaatctcg cccagcttgt ccagcgcttt ttcaggatct gtgccggtaa aagcccgggc
     4381 ctcttcttca ttggcaaaaa cgatatctac gtattctgcc aggattattt tcaaaaaatc
     4441 caggttggct tctaccacgt tgtaactggc caggtcaagg caaattttac aatgatgctg
     4501 atcggcatat tccagaattt tttcaacgag ggcatgattt tgcaagagat atccttcgat
     4561 atagatataa tcgaattctg aaaacatatc tgcagcaaga tcttcttgcg agagctcgat
     4621 ggcagcgccc aggtaggtgg caaaggtgcg ttccgaatcg gggctgataa gagctatggc
     4681 gcatccggta tcggaagagc cggtaagcag tatcgggttg atgcccgtgt tacgcatatc
     4741 gttggcaaaa aattcgccgt atttgtcgtt gccggtttta ccaataaagg aggtggtggc
     4801 accaagattt gccagtccgt gaatggtgtt tgccgcagaa ccgccggatg ataactctgt
     4861 tttgaatttt tcagaacgct ttaaaatgga cgccgaagtg tcgatgtcca ccagttgcat
     4921 gctgcccttg gggatattta tctgttgcag aaactcttcg ttatcaagga tggtcattat
     4981 gtcaacaagc gcgttgccaa ttcctaaaat ctttttcata ctatgggtga ttttttttta
     5041 acaggtgcaa aggtaggcaa aaattacttt ctgaggtaca ggtcgtcctg tttaatcgga
     5101 taaatctttt tttgaggcgg catatttaaa taaagacttc tcctttctta atcatagacc
     5161 gattaaatgt ttgcacgtag caccaatggt tgtcaatagc ggcgcgatgg gccgtcaaaa
     5221 cggcgggctg gcgccggcgt gaatcagcag gacggtgaaa atcagcaaag gcagcccggg
     5281 ctaccgggct gtttgtctga aatggcttgc attaactgct gatgaataca ggcactattt
     5341 cttacccaaa aataattcaa actgttgttg gcctttgatg gccttttcag acactcgttt
     5401 gcatgatttt cccgacctga tgagagcggg cagaagcata cgttttgaca aggttttttg
     5461 gtaacttttt ttccgaaaaa aagttacatg aacatgtgct tgcactgagt ggcaaaacac
     5521 gagcttcggc atatgcaagt atgtgtatga ctgcgcgcgg ccatgcgcgg aagcaggtct
     5581 tgctaacact gaaacattat tacatttttt tggtactttt ggcttgaccc aaaagtaccc
     5641 caaaggtcaa gaaaatatat tgcgagcctt cgcacgggcc actccctgac accgccatat
     5701 tttctggcca ccgcactgtt gctgctacgc agcaattcga acc
//
LOCUS       DAOSFP010000128         5721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12423_length_5721_cov_3.203318, whole genome shotgun sequence.
ACCESSION   DAOSFP010000128 DAOSFP010000000
VERSION     DAOSFP010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12423_length_5721_cov_3.203318"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1549)
                     /locus_tag="PLI16_04990"
     CDS             complement(<1..1549)
                     /locus_tag="PLI16_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83948.1"
                     /translation="MNLQNAFSQCTNCNSNYPTGTITPSSATWTLYGSGSTLSGGYQF
                     FTLSTTYIYQFSSYGSGANSQLTLYPSSTCDAAYSLAYNDDYDVSGDMDGSISPYNQS
                     AVIAYYPGTTDVRLLLSAYNCTTGSASFNGYYRAIPYNPSAYSASATAICTGGSVTLT
                     APSADVSPTENDAVLNIQWSTNNFASVNVSADANSVVVSPTATTTYYMRYQVLAGNNT
                     YYSTAVSQTITVTPNNTITLTAGGTQTKCINTPITTTTYSTTGATGATITGLPAGVTG
                     SWASNVVTISGTPTVSGAFTYTVTLTGGCGTVTANGTITVTPNNTISLTAGGTQTKCI
                     NTAITTTTFTTTGATGATITGLPAGVTGSWASNVVTISGTPTVSGAFTYTVTLNGGCG
                     TVTANGTITVTPNNTISLTAGGTQTKCINTPITTTTYATTGATGASVTGLPTGVTGSF
                     ASNVVTISGTPTVSGAFTYTVTLTGGCGTVTANGTITVTPNNTISLTAGGTQTKCINT
                     AITTTTYT"
     gene            complement(2269..4788)
                     /locus_tag="PLI16_04995"
     CDS             complement(2269..4788)
                     /locus_tag="PLI16_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83949.1"
                     /translation="MKKNKLFAIVMILSMLCMDVFAQSGANDPSFDPTYQGFDAGTGA
                     NRAVYSTSLQTDEKIIIAGSFTYFNGSSRRRIARLNTDGSIDITFDPGSGASQNSTIY
                     TTSLQSDGKIIIGGNFTSYNQVQRSRVARVNTDGTLDGTFNPGSGTGMNSTVYASAIQ
                     SDGKIIFGGYFSSYNNKSRNCIARANANGGADTTFTPGTGANNTIYAVAIQTDGKILI
                     GGSFTSFNGTSINRIARLNADGSLDASFVPGTGAGGTIRTVALQSDGKIVIGGNFTSF
                     NGTTVNRIARLNSDGTIDNSFVPGAGASAYVRKAVIQTDGKIVIGGEFVTFNAVSKKR
                     IVRLNSDGSMDASFLPGSGADSNIVAMAVKGDGKIIIGGDFTFYDGTSRNYFARLNSD
                     GSLDSTCVNCSGANEVVRSANIQNDAKIIIAGYFTFFNGTGINRVARLNTDGSLDDTF
                     DPGSGPDGGVTTSILQGDGRIFIAGNFESVNGTPLYRVARLNTDGSVDVSFTPDSGAN
                     KIIYAAAIQSDGKIVIGGEFDSVSDTPRSRIARLNSNGTLDVTFNPGTGADSYITAIA
                     IQSDGKIIIGGMFTSYNWTTSNYIARLNSDGSFDATFNTGAGANGIIRTVTIQSNGKI
                     LIGGSFSTYNGISVNRVARLNTDGSIDASFNPGGAGASGSVYSILAQNNGKIYISGIF
                     TSYNGTSRNRIARLNIDGTLDNAFDPGTGSNNIIFTSALQADEKIIIGGGFTTYNGAD
                     RYRIARIMNTVVVGTTDDYSGNNISVYPNPTLGSVVITSPEEMKNAKIKVFDIQGRLV
                     LQKENFNGYSCSVNLENYGKGLYFIEIGQGDSFKRMKLIRN"
     gene            complement(4899..5576)
                     /locus_tag="PLI16_05000"
     CDS             complement(4899..5576)
                     /locus_tag="PLI16_05000"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HOH83950.1"
                     /translation="MKKIHFSVVALMLVILFSINSTAQTQDANLWMNVAIEKKITPKF
                     SIGFSQEFRMNENISELGTFFTDLCFEYKISKIIRVSANYRFVNKRRLDDSYSKRHRY
                     YFDLTFRKKFSPVIISLRTRFQSQYKDVLSSNDGKIPTNTFRNKLTIKYNAEKKLQPY
                     IFAEIYTPLKQPYDIFIDNTRYCAGVEYEINRMHTLDFYYLFQKEYHVKNPVSDHVFG
                     IGYHISL"
     gene            complement(5573..5719)
                     /locus_tag="PLI16_05005"
     CDS             complement(5573..5719)
                     /locus_tag="PLI16_05005"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOH83951.1"
                     /translation="MNKNRSEELIADLRKRTGLEVHRFEVEKIDFLKDAARLTIYYYN
                     KKNK"
BASE COUNT         1669 a         1139 c         1166 g         1747 t
ORIGIN      
        1 tagtataagt ggttgttgta atcgcagtat taatacattt tgtctgtgta ccacccgctg
       61 ttaaagagat cgtattgttt ggtgtaaccg ttatggttcc attagcagta actgtaccac
      121 aaccaccggt aagggtaacg gtataggtaa aggcacctga aaccgttggg gtaccactga
      181 ttgttaccac gttggaggca aatgatcccg ttactccggt cggcaacccg gtaacggagg
      241 ctccagtggc acctgtagtg gcataggtgg tagtggtaat tggcgtattg atacactttg
      301 tctgggttcc gccagctgtt aatgagatgg tattgtttgg tgtaaccgtg atggttccat
      361 tagcagtaac tgtaccgcaa ccaccattaa gggtaacggt atacgtgaag gccccggaaa
      421 cagtaggcgt accgctgatg gttaccacgt tggaagccca tgaaccggta acgccggcag
      481 gtaatcctgt aatggttgct ccggtagctc ctgtggtagt aaaggtggtt gttgtaatcg
      541 cagtattaat acattttgtc tgtgtaccac ccgctgttaa agagatcgta ttgtttggtg
      601 taaccgttat ggttccatta gcagtaactg taccacaacc accggtaagg gtaacggtat
      661 aggtaaaggc acctgaaacc gtaggtgtgc cgctgatggt taccacattg gaagcccatg
      721 aaccggtaac gccggcaggt aatcctgtaa tggtggctcc ggtagcccca gttgtggagt
      781 aggtggtagt ggttattggc gtgttgatac actttgtttg tgttccccct gctgttaaag
      841 tgatggtatt attaggtgta accgtgatgg tttgtgaaac tgcagtacta taataggtat
      901 tattccctgc aagaacctgg tatctcatat agtatgtagt ggtagctgtc ggagaaacga
      961 cgaccgaatt ggcgtcagca gagacattga cactggcgaa gttatttgtt gaccattgta
     1021 tgttcagcac agcgtcattt tcagtaggac taacatctgc tgaaggcgct gtaagtgtca
     1081 ctgaacctcc agtacatatt gctgttgcag atgctgagta ggcagacgga ttataaggga
     1141 ttgctctgta ataaccatta aaacttgcgc tgccggttgt acaattgtat gcactgagta
     1201 acaaccttac atcggtggta ccagggtaat atgcaatgac agcagactga ttataaggcg
     1261 agattgatcc atccatatca ccacttacgt cataatcatc attataggct aacgaatatg
     1321 ccgcatcgca tgtagatgaa ggatataatg tgagttgaga gttagcaccc gatccatagg
     1381 atgaaaactg ataaatataa gtagtagaca atgtaaaaaa ctgataacca cctgaaagtg
     1441 tggaaccgct tccatatagc gtccaggtag cactcgatgg ggtaatggtt ccagttgggt
     1501 aattcgagtt gcagttagta cattgtgaaa aagcattttg caaattcatt aaactaattg
     1561 ctgtcaagaa taaaaaaaaa gagtaagttt ttgaatgact tcatattttt gggtttttat
     1621 ttttaacata aattcgcttc aaatttatct tttttttgat caataattca acaattattt
     1681 tatttttttc taacttaaat gatgtatcaa aataaacaag tattataact acccagtatt
     1741 ttgaattgcc aaattaatat cttgatgatt gtgcaagaga tagtttttat gtataatata
     1801 ttgaataaca aacattaaga gattttattc agcgagaata tgtcgcgtag atgcattgtc
     1861 agcatactat tttttgtcgt ttaccagtga ctgttattta cacaacatat attattataa
     1921 taatatttta tattattttt tacaactaaa tatcaatcgc aaattatttg aaataagcaa
     1981 tgcgtcaaac tttgtaatac agaagctaat tcactcgttc aatgggtgaa aaataacacc
     2041 aattgtaatt atttttagtt aaaaacaaaa aattaccatg cctgcttcgc ccgtcaaaca
     2101 gggttaatgc tagttctaca taaaaaccgt acaacaagaa aagtcgatat tggactatcc
     2161 gcctgccggt gaatgattgc ccaatcggta ttatattcta taacaacacc tgcataaaaa
     2221 aagctccatt tagatgaagc ttttttaaaa aaaattgttt gcagtttctc aatttctgat
     2281 aagcttcatt cttttgaaac tatcaccctg ccctatttcg atgaaataaa gtcccttgcc
     2341 ataattttct aaattaacag agcaggaata tccgttaaaa ttttcttttt gcaataccaa
     2401 tctaccctgg atgtcaaata ccttaatttt tgcatttttc atttcttcag gtgaggtaat
     2461 tactacagaa ccaagagtag gattgggata aaccgatata ttgttcccgg aatagtcatc
     2521 ggtcgttccc actacaacgg tattcatgat acgagcaatt ctgtacctat cagctccgtt
     2581 ataggttgtg aaaccaccac cgataatgat tttttcatct gcctgcaagg cagaagtaaa
     2641 aataatgttg tttgaccctg ttcccggatc aaaagcatta tccagggtac cgtcgatgtt
     2701 gaggcgggct atgcggtttc gggatgttcc gttataggag gtaaaaatac ccgaaatgta
     2761 aatcttgcca ttattctgag caagtattga atacacagaa ccgctggccc ctgcaccacc
     2821 aggattaaat gaggcatcaa tactgccatc ggtattcagg cgggcaaccc gattgacgga
     2881 gattccgtta taagttgaaa aactcccacc gataaggatt tttccattgc tctgaattgt
     2941 aacagtccgg ataataccat tggcgccggc gccggtatta aaagtggcat cgaagcttcc
     3001 gtcactgttc agccgggcta tgtagttact tgttgtccag ttgtatgaag taaacattcc
     3061 gccaataata attttaccat cactttgaat agcgatagct gtaatataac tgtcggctcc
     3121 tgtgcctgga ttaaaagtga cgtcaagtgt tccattgctg ttgagacgtg caatgcgtga
     3181 acggggtgtg tcgctaaccg aatcaaattc accaccaata acaattttcc cgtcgctttg
     3241 tatggcagcc gcataaatga ttttattggc gccactgtcg ggagtaaacg aaacatcaac
     3301 actgccatcg gtattcagcc gggcaacacg gtataatggc gtaccattaa ccgattcgaa
     3361 attgccggca ataaaaattc ttccatctcc ctgaagaata gaagtggtaa caccgccatc
     3421 agggcctgaa cccgggtcaa atgtatcatc caggctgcca tcggtgttta accttgcaac
     3481 acggttaatt cctgttccat taaaaaaggt aaaataacct gctataataa tttttgcatc
     3541 attttgtata ttggcgcttc gcactacctc attagcccct gaacagttaa cacatgttga
     3601 atcgaggctg ccatcactgt tgagccgggc aaaatagttt ctggatgtac cgtcgtaaaa
     3661 agtaaaatct cctccaataa taatttttcc atcacctttc actgccatgg ccacaatatt
     3721 gctgtctgcc cctgatcccg gcagaaagga agcatccatg gagccatcac tgtttaagcg
     3781 gactattctt ttctttgata ccgcattaaa ggtaacaaac tctccaccga taactatttt
     3841 tccatccgtt tgtataacag ctttacgtac ataagcgctg gctccggcac cggggacaaa
     3901 agaattgtca atagttccat cactgttaag cctggcgata cgattcaccg tggtcccgtt
     3961 aaatgaggta aaatttccgc ctatgactat tttcccatca ctttgaagtg ccactgtacg
     4021 tatggtgcca ccggctccag ttccagggac gaaggaggca tcaaggctgc catcggcatt
     4081 caaacgggct atacgattaa tggatgtgcc gttgaaagag gtgaaacttc cgccaataag
     4141 aattttgcca tcggtctgaa tagccactgc ataaatggta ttatttgccc cggtaccagg
     4201 agtgaacgtt gtgtcagcac cgccgttggc attagccctg gcgatacaat ttcgcgactt
     4261 gttattataa ctgctgaaat aaccgccgaa tattatcttg ccatcgcttt gaatggctga
     4321 agcatagacg gtgctgttca taccagtacc cgagccggga ttaaaagtac catccagggt
     4381 tccgtcggtg ttaacacgtg ctaccctgct tcgttgaacc tgattgtatg atgtgaaatt
     4441 acctccgatt ataatctttc catcactttg cagggatgta gtataaattg ttgagttctg
     4501 actggcgccg ctacccggat caaaggtgat atcaatactg ccatcggtat tcaacctggc
     4561 aattcttctt cgggaagaac cattgaaata agtaaaactg cctgcaatta ttattttctc
     4621 atctgtttga aggcttgtgg aatatacagc cctgtttgca cctgtgccgg cgtcaaatcc
     4681 ctggtaagtg ggatcaaaag aaggatcatt ggcgcctgat tgggcaaaca catccataca
     4741 aagcatggat aaaatcatta ctatagcaaa gagtttgttt tttttcattt gaattatatt
     4801 ttttctaaaa aaaaatttat ttatgatgca aaagtatgat tactaaccaa aaaatcagta
     4861 aaagatacct ttttgtttaa atttttttta aatacgattt ataaacttat atgataccct
     4921 atcccaaata catgatcaga taccggattt ttcacatgat attctttctg aaataaataa
     4981 taaaaatcaa gggtatgcat cctgtttatt tcatattcga cacctgcaca ataacgcgta
     5041 ttatcgatga aaatatcata gggttgcttc agcggagtgt atatctcggc aaaaatatat
     5101 ggctgcagtt tcttttctgc attatatttt attgtcagtt tatttctaaa agtgttcgtg
     5161 ggtattttac cgtcattgct tgagagcaca tctttatact ggctttggaa acgggtacgg
     5221 aggcttatga ttaccggact gaactttttc cggaaagtca ggtcaaaata gtagcggtgc
     5281 cttttactat aggagtcatc cagcctgcgt ttgttgacaa aacgataatt tgcagacaca
     5341 cggataattt tggatatttt atattcgaaa caaagatcgg taaaaaacgt gcctaactcc
     5401 gaaatgtttt cattcatcct gaattcctgc gaaaagccaa tcgaaaattt aggagtgatt
     5461 tttttctcaa tagcaacatt catccacaaa ttggcatcct gtgtctgggc agtactgtta
     5521 attgaaaaca gtataaccag cataagagcc actacggaaa aatggatttt tttcatttat
     5581 tttttttatt gtaataatat atggttaatc gcgcagcatc tttaagaaaa tcgatttttt
     5641 ccacctcaaa acggtgaacc tcaagtcctg tacgttttct caggtcagca atcaattctt
     5701 cacttctgtt tttattaatc a
//
LOCUS       DAOSFP010000129         5642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12673_length_5642_cov_3.583139, whole genome shotgun sequence.
ACCESSION   DAOSFP010000129 DAOSFP010000000
VERSION     DAOSFP010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12673_length_5642_cov_3.583139"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2995)
                     /locus_tag="PLI16_05010"
     CDS             complement(<1..2995)
                     /locus_tag="PLI16_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH83952.1"
                     /translation="MKKLFLLILITIQWPLIIFCQSPELVLPTGHSDGILHAAYSKNG
                     KYIMTASMDKTARIWDAYSGKLLSVLYGHTDYVNRIQSTDDSRLAITASDNTIKVWTL
                     PQGILKYDFKCESCFSGVLGSDPQNNALVIATGKGNLAVYNLKTGLLVKELKCHDKRI
                     RRIVFSPDSKTLLTAAYDEKFVKAINIENGSVLFEINYQEWEPEFDFINSGNEILVKG
                     DQGRVYNALTGTFLRMDENYKKSQEPMEAFSPDNRYYVYYGIPEASNFQNFASVHDTK
                     TGRELRLLTGRVPEITDIQLSTDKNFLASLHDDKSARVWNITNGTPAAVLLEDSVWRP
                     EVWGGAAAFFRDGKKLVTVFYDSCKATVWEVNSQSLVHTTPAVDDELLRNFDLHFTNC
                     AFNEDSSKLLISNWGCVSVIDAKTYKQLFQKSEVHLSSAEWTDHGKKLVIKYIGLEPE
                     VWCAEKWVLLHRFNNEMKDEGGYIENFRVCKNGKQGVSLSSSKKVGLWDINSWRLVYS
                     TPDSHTLTFVDYNEAHNYIITIDTGHNIQLIDPESGKYIRTFSGHQGIAYTIEFSNDG
                     SEMMSFSEDAKEVILWNVATGEMINRISPSERPHCFFCYEDKYFAHNQLEILCYEKKQ
                     HEIKFRMAAIQDHGYVCLLKNGKYRCSRDALKYLAWKVNDRIYGFDQWDVIYNRPDEV
                     MKALGSSEDNFIQAYYQAFLKRLKKMNADSSANQIGKDYPEIIVFNADAVQGMSKDSL
                     INLEIRAVDKSDKGFLTKIVVMVNNCPIGDPDKKLSFTEKQKDVRVTVPVSLSYGENT
                     IEVFSLNSRNNSSLKQRMLIDYLPAKKPKSVKHLIIISVSAYQDTKYNLKYAVKDGRD
                     MAALFGGRGAVVDTLFDQNATRPDILALKQKLLQTGVDDEVVLYVSGHGLLDKNYDFY
                     FAAYDMDFADPAKRGVLYDDLEALLDGIPARKKLLLMDACHSGEVDKEQLSVNVPVTN
                     PGNNKRGDIKTYGYKGATIEEEGSGL"
     gene            complement(3049..3864)
                     /locus_tag="PLI16_05015"
     CDS             complement(3049..3864)
                     /locus_tag="PLI16_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83953.1"
                     /translation="MLTRLFLYGLFLLIICPATAQTDGLQFKVMAVHGKVFHNGNPLK
                     KNDVFTVYKAGELVTGFGYGGPDDWLKVMEINSGATRTCYAQQQKAGKGHYLFTRSSE
                     DITNDNELIYFFNRPCIFLFEDDTLLCYGLRRFKTDGNRLLNVQYKARGNSITKTIGR
                     NDSLILSREIFTTENGRISSAGVDSIQLTYYDASSNKTTCPEVSPFCIYFFSDIVLQL
                     KQMGLKQQEVFDELTEHFVSLDEIKKDRGVRNKAEAEAWLKEEIEKNWKKPKR"
     gene            complement(3858..5120)
                     /locus_tag="PLI16_05020"
     CDS             complement(3858..5120)
                     /locus_tag="PLI16_05020"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOH83954.1"
                     /translation="MLTPKRIRKVISRVICVFLPGITLKRLLANTVHAVWMIAFSLLW
                     LSHVNMYDGFDFMNEVMDYKELLSKHILNKKPELLPTEKYLLLNTSKNNEILPADNDN
                     TLNTVITDREALLKSLQLLDSNSDKISLVICDVFFESPSSNRLADSLLGAVVQRLDAK
                     NKLILPALYYDDDASLQLPLFSCRTGVSQYRSSFLNEQFLKYSFMLDDTLKQLPLIAY
                     ENITGHRMEKKRRLCFPYYTMNGKWCLNTIIPEIRYAADALEEDVSYHHIGTFSREML
                     KDNQIVVIGDFEGKYDVHSTIADNISGALILINALEALMNGDNIIGPRYLLMLFVFFF
                     FVGYYTFFNKDLEHQNQKFINKSPFFRFLVVQSNYLLLLLLVLISMLYFHHYIHFLIL
                     MSYFMVIDIVLYLINQCRSKKQKVKKSC"
BASE COUNT         1586 a         1220 c         1108 g         1728 t
ORIGIN      
        1 caaggccgga accttcttcc tcgatagtgg cgcctttgta gccgtaggtt ttaatatcgc
       61 ctcttttgtt attgccggga ttggtcaccg gaacattaac cgacaactgc tctttgtcaa
      121 cttcaccgct gtggcatgca tccatcagca agagcttttt gcgggcggga atgccgtcga
      181 gcagggcttc caggtcgtcg tacaacacac ctcgctttgc aggatccgca aaatccatat
      241 cataggcagc aaaataaaaa tcgtagtttt tatccagcag gccatggccg gatacatata
      301 aaaccacctc atcgtccacc ccggtttgta gtagcttttg tttcagggca aggatgtccg
      361 ggcgggtagc attttggtcg aacagggtat ccacaacggc gcccctgccg ccaaaaagcg
      421 cagccatatc gcggccgtct ttcacggcat atttcaggtt atattttgta tcctgatagg
      481 cagagacact gataatgatc agatgtttta ccgatttcgg ttttttagcc ggcaggtaat
      541 caattagcat ccgctgtttc agagaactgt tgtttcggct gttcagagag aatacttcta
      601 ttgtgttttc gccatacgac aggcttacgg gcacagtaac ccggacatcc ttttgttttt
      661 cagtgaatga cagtttttta tccgggtcgc cgatggggca attattgacc ataacgacaa
      721 ttttagtaag aaatcctttg tcggacttgt caacagccct gatttcgaga tttatcaggg
      781 aatctttaga catgccctga acagcatcgg cgttaaaaac aataatttcg ggatagtctt
      841 tgccaatctg atttgcagaa gagtcggcat tcatcttttt cagccgtttc agaaaagcct
      901 gatagtatgc ctggataaaa ttatcttctg aagaccccag tgctttcata acttcatcgg
      961 gccggttgta gattacatcc cactggtcaa aaccataaat tctgtcgttg accttccagg
     1021 caaggtattt taaagcgtcc cggctgcacc ggtattttcc gtttttcaaa aggcacacat
     1081 agccatgatc ctgaatagcc gccatgcgga attttatctc atgttgtttt ttctcataac
     1141 acaaaatttc cagctgatta tgggcaaaat atttatcctc gtaacagaaa aaacaatgag
     1201 gtctttctga cgggctaatc ctgttgatca tctctcctgt cgccacattc caaagtatca
     1261 cttcttttgc atcttcggaa aacgacatca tttcggagcc atcattgctg aattcgatag
     1321 tgtacgctat gccttgatgg cctgaaaaag ttctgatgta ttttcccgat tcgggatcta
     1381 tcaattggat gttatgcccg gtatcaatgg taataatata attatgggcc tcattgtaat
     1441 ctacaaaagt gagggtgtgg ctgtccgggg tagaataaac taatctccag gaattgatat
     1501 cccaaaggcc gactttcttt gatgaagaaa ggctgactcc ctgttttccg tttttgcaca
     1561 ccctgaaatt ttcaatatac ccgccttcgt ctttcatttc attattgaaa cgatgtaaaa
     1621 gcacccattt ttcggcacac cacacttccg gttccaggcc tatatattta attaccagtt
     1681 tctttccatg atctgtccat tcggcggatg aaagatgtac ttcgcttttt tggaacaatt
     1741 gtttgtacgt ttttgcatca atcaccgaaa cgcaacccca gtttgaaatc agcagtttgc
     1801 tgctgtcttc attaaaagca caattggtga aatgaaggtc gaaattccgc agcaactcat
     1861 catccacggc cggcgtggta tgaactaggc tttggctgtt aacctcccat accgttgctt
     1921 tgcagctatc ataaaaaacg gtaacaagtt tttttccatc acggaaaaat gccgcagccc
     1981 ctccccacac ttctggtctc cacaccgaat cttccagaag gacagcagca ggggtcccgt
     2041 tggtgatatt ccaaaccctt gccgatttat catcatgcaa cgaagcaaga aagtttttat
     2101 ctgtacttaa ttgtatgtcg gtaatttctg gcaccctgcc ggttaataat ctcagctccc
     2161 tgcctgtttt cgtatcgtgc acgcttgcaa agttttgaaa attacttgct tcagggattc
     2221 catagtaaac atagtatcta ttatcaggac taaatgcttc cattggttcc tgagattttt
     2281 tatagttttc gtccattctt aaaaacgtgc cggtgagggc attgtacacg cgtccctgat
     2341 caccctttac taagatttcg ttacccgaat taataaaatc aaattctggt tcccattcct
     2401 gataattgat ttcaaacaac acgcttccat tttcgatgtt gatggctttc acaaactttt
     2461 cgtcgtaagc agcggtcaat aatgttttac tatcgggact gaatactatc cttcgtatcc
     2521 ttttatcatg acatttgagt tcctttacca ataaccctgt cttgagatta taaacagcca
     2581 gatttccttt tccggtagct atcaccagtg cgttattttg cggatcggat cccaaaacac
     2641 cactgaaaca actctcacat ttaaaatcat atttaaggat tccctgaggc aaggtccaaa
     2701 ccttaatagt attatcggag gccgtaattg ccagcctcga atcatcggtc gactgaatcc
     2761 tgtttacata atcagtatgt ccataaagta ccgaaagtag tttcccgctg taggcatccc
     2821 atattctggc agttttatcc attgatgccg tcataatgta tttgccgttt tttgaataag
     2881 cggcgtgcaa tatgccatcg gaatggcctg tgggtaaaac aagttcagga gactgacaaa
     2941 atattatcag tggccattgt atggttatga ggattaacag gaataacttt ttcatttgtt
     3001 gagcatatgc ttactgataa tgacaatatt tttcggtttt tccggttttt accgttttgg
     3061 ttttttccag tttttttcaa tttcctcctt cagccaggct tctgcttcgg ctttattcct
     3121 gacccctcta tctttcttta tttcatccag gctcacaaaa tgttcggtga gttcatcaaa
     3181 cacctcttgc tgcttcaagc ccatctgctt caattgcaac acgatatcgc taaaaaaata
     3241 aatgcagaac ggactcactt caggacaggt ggttttattg cttgaggcat cgtaatacgt
     3301 gagttgtatg ctgtcgacac cggcggatga aatgcggccg ttttctgtag taaaaatttc
     3361 ccgggaaaga atgaggctgt catttcttcc aatggttttt gttatgctgt ttccccttgc
     3421 tttgtattgc acgtttagca ggcgattccc gtcggtttta aatctacgga gtccataaca
     3481 caacagggtg tcgtcttcaa ataaaaaaat gcaaggccgg ttaaagaagt aaattaattc
     3541 attgtcgttt gtaatgtctt ccgaagaacg cgtaaacagg taatggcctt tgccggcctt
     3601 ttgttgctgg gcgtaacagg ttcgagtggc cccactgttt atttccatca ccttgagcca
     3661 gtcgtcaggc cctccataac cgaaaccggt aaccagttcg cctgccttgt aaacggtgaa
     3721 gacatcgttt tttttgaggg gattgccatt atgaaaaacc ttgccgtgaa cagccatcac
     3781 cttaaattga aggccgtcag tctgtgctgt cgccggacaa ataatgagca aaaaaagtcc
     3841 gtaaagaaaa agtcttgtca acatgatttt tttacttttt gttttttact tctacattga
     3901 ttgatcaggt ataacacaat gtcaatcacc ataaaataac tcatcaatat cagaaagtga
     3961 atataatgat ggaaatacaa catcgaaatc aacaccagca gcaacagtaa aagataattg
     4021 gactgaacca ccaaaaagcg gaaaaaggga cttttattaa taaacttttg gttttgatgt
     4081 tccagatctt tattgaaaaa agtataatag ccaacaaaga aaaagaaaac aaatagcatc
     4141 agcagataac ggggcccgat gatgttatcg ccattcatca gggcttccaa cgcattgata
     4201 agtatcagcg ccccggaaat attatcagct atggtgctgt gcacatcgta ctttccctca
     4261 aaatcgccga tgacaacaat ctgattatcc ttcagcattt ccctgctaaa ggtgccgata
     4321 tgatgataag aaacatcctc ttccaaagca tctgctgcat accgtatttc aggtatgatg
     4381 gtgttcaggc accatttacc gttcatggta tagtaaggaa aacaaagccg gcgtttcttt
     4441 tccatcctat gcccggtaat attttcatac gctatgagtg gcaattgttt taaagtgtca
     4501 tccagcataa aggaatactt caggaattgt tcatttagaa aagaagaccg gtattgcgac
     4561 accccggttc tacatgaaaa taatggcaac tgcagggaag catcatcatc gtaatacagt
     4621 gcaggcaaaa tgagtttgtt tttcgcatca aggcgttgaa cgacggctcc cagaagactg
     4681 tcggcaagcc ggtttgagga tgggctttca aaaaacacat cgcagatgac cagagaaatt
     4741 ttatccgagt tgctgtcaag tagctgcaag ctttttagca gggcttctct gtcggtgatg
     4801 acagtattga gcgtgttatc gttgtcggca ggaagtattt cattattttt tgatgtattg
     4861 agcagcaaat atttttctgt gggcaacagt tcaggttttt tgttcagaat atgtttactc
     4921 aacagttcct tgtagtccat cacctcgttc ataaaatcga aaccgtcgta catattgaca
     4981 tgagacagcc acagcagcga gaatgcaatc atccacactg cgtgaacggt attagccaac
     5041 aagcgcttca gggtgatgcc gggcagaaaa acacagataa cacggctgat gacttttcgg
     5101 attcttttag gtgttagcat acaagaattt gtttttttgt ttttctgctg ttttctttgt
     5161 ttgctaaagc tgttcatcaa atagaaaaaa atgagttatg gcggaccgca acaaggacac
     5221 aaacaactga ttattaataa tttatataca taatattttc attaattccg tactctaaat
     5281 ttcagagcga agtagcatgt tacccacaat gccatttcga gcggaattaa tgcatgcaaa
     5341 ttaatgaaat tttttatatt gagtagaaaa aataacaaat attcaacttg tttaatcata
     5401 tttttaaaaa atgtatgttc attattccaa aagcccatcg tttacatgat aaaactccac
     5461 actaccagtt cacgaaaatc gttgaaaaag ccgtttataa aactgaaacc agttatgtga
     5521 agttgctgaa cttctcaccg gataaatgtt attccttgac taaaagaaaa aacaaactac
     5581 ttatactgat tggaaaaata atatagtcaa aactttgcac aaaagaggaa cacttgtatt
     5641 gt
//
LOCUS       DAOSFP010000130         5614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12772_length_5614_cov_3.603526, whole genome shotgun sequence.
ACCESSION   DAOSFP010000130 DAOSFP010000000
VERSION     DAOSFP010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12772_length_5614_cov_3.603526"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLI16_05025"
     CDS             complement(<1..368)
                     /locus_tag="PLI16_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOH83955.1"
                     /translation="MATKKKVLLIATNTEKKPYPVPPIGLGLVAQSIREHYDVKVYDA
                     MFDEGRSLPEVVNSFRPDYIGCSIRNVDSMSVEAPDFFPEAINEKIIRPLKKITDAPI
                     ILGGSGYSIYPYEMLDYFDL"
     gene            complement(470..1861)
                     /locus_tag="PLI16_05030"
     CDS             complement(470..1861)
                     /locus_tag="PLI16_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83956.1"
                     /translation="MKVTSLLYSIIYFAATAIILCIPALYNGYLLFDCDSALYVAQGI
                     KLTPLVDRPIGYGYFIRATTWQSTLWMVIFSQGLIASLLIYHALKTVLIKITFNKLYH
                     FITICFLSLFSTLGWFVSMLMPDILTSFLFLALFNILFGKNCALSYILYSVLIFFIVF
                     SHLSNIPVLALLLITVWLFILSKRKFAEFKRKALLGSIMIMLVYLGTISCIIKYNYDH
                     FKKPCLSTTSGIFFFARLIDTGVVDTYLKENCDKKDYRICKYKDDIPKWSQLFIWDTK
                     GIFQKMGGWNNYQEEPKLIVKDILSSPKYYGPILWNFSLSTCKQSFQFRTGDGMINFL
                     NEQWYPVYDNTYKNFPKNEIKRDFYNSKQLTEGLKFETINNVIYFIVILSILLIACVL
                     YRFKIDDKIYLFLVICFSGVFFNAAVCANLSNVLNRYQARVVWIIPFIAIVLFLNYLL
                     PKIITFLRKENLA"
     gene            1928..2062
                     /locus_tag="PLI16_05035"
     CDS             1928..2062
                     /locus_tag="PLI16_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83957.1"
                     /translation="MGLLPGSFTPGCKFVLRTKQNNIGKDSEIKLGGQIENIRIQGTG
                     "
     gene            2203..3636
                     /locus_tag="PLI16_05040"
     CDS             2203..3636
                     /locus_tag="PLI16_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOH83958.1"
                     /translation="MKEEFVNNSAALFERRFGEKPISIDELPSSGSNRCYIRVVSENN
                     KALAAYNTDRKENIAYLSFARHFEKHKLPVTKIFAEDLDNHLYLLEDLGDETLFAYLE
                     KHRAEGFSNTTVGLYKEVLDNLLRFQIDAAADLDYSVCYPRAAFDRQSMMWDLNYFKY
                     YFLKLAQISFDEQKLEDDFNVFVDYLLQADADYFMYRDFQSRNIMIRDGKLYFIDFQG
                     GRKGALQYDVASLLYDAKAAIPQQVRDELLDYYIARLSQKKQIEESEFRRQFAGFVLV
                     RIMQAMGAYGFRGYYEKKTHFLKSIPYAVNNLKWILKNAGLPVKTSILQDVFEQIIAS
                     KNLQRYVDEKTPGLTVRINSFSFKKGYPEDPGGNGGGFVFDCRALPNPGRYDEYKGLT
                     GMDKPVIDFLEHEPETAVFFENTSQLVSQSVENYMKRGFTHLMVSYGCTGGQHRSVYF
                     AEKLAAYVREKFDAAIIVNHKEQNTKP"
     gene            3633..4346
                     /locus_tag="PLI16_05045"
     CDS             3633..4346
                     /locus_tag="PLI16_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOH83959.1"
                     /translation="MKAMILAAGMGTRLGALTAEKPKALIEVNGRTLLEHVVENLKRF
                     GVKEIIINVHHFGGQIIDFLKQKKNFDIRIEISDESDALLDTGGGLMKASYFFDDGKP
                     FLVHNVDVLSHIDLHKLEIYHTHIRSLATLVVKHRETSRYFLVDKYQLLCGWKNKKTG
                     EKMVVRYGEKLTEAAFSGIQMLSPEIFRLCNKKGAFSLTEMYLDLAAENKIVAYNDGS
                     LWFDLGKPENIAEAAKHFV"
     gene            4388..5602
                     /locus_tag="PLI16_05050"
     CDS             4388..5602
                     /locus_tag="PLI16_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018967296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH83960.1"
                     /translation="MKIAVVGGGAAGFFSALTAKENFPQAQVYIFEKARRLLSKVRIS
                     GGGRCNVTHACPTVEDLCAAYPRGGRKLKKAFHIFNNTHTCAWFEARGVPLVVQDDNC
                     VFPASQNSQSIIVCFMREAERLEIHISGGVGVTALTAGDKGLELSFDVPGRQPEVFDK
                     VIVASGGSPKQEGLQWLEALGHKIETPVPSLFTFNMPGESVTKLMGVVVPDVVAGIQG
                     TKLRAAGPLLITHWGMSGPAIINLSSLGARLLNEKNYSFVVQLNWVNQQNTLAVAENL
                     QRIASAHAARAIHNFRPYDLPSRLWEYLLAKCGMAHDKTWEELGKKGLNKIVNVLTND
                     TYQVQGQTTFREEFVTCGGVSLDSIDLNTMQSKVCRNLYFAGEVLDIDGITGGYNFQC
                     AWTTGFIAGKLQ"
BASE COUNT         1702 a         1084 c         1259 g         1569 t
ORIGIN      
        1 tccaaatcaa aataatccag catctcgtaa ggatatatgc tgtacccgct gccgccgaga
       61 atgatgggtg cgtcggtaat ttttttcagc gggcggataa ttttttcgtt gatggcctca
      121 ggaaaaaaat caggggcttc caccgacatc gaatccacat ttctgatgct gcagccaatg
      181 taatcgggcc tgaaagaatt taccacctca ggcagcgacc ggccttcatc gaacatagcg
      241 tcataaactt tcacatcgta gtgttcccgg atactctgtg ccactaagcc aaggccaata
      301 ggggggacgg gatatggttt tttttcggtg ttagtagcta tgaggaggac ttttttcttt
      361 gttgccaact tcttcagatt atttatagcg caaaattaca acgaaaatta ttatatccgc
      421 agtttctttt gttgttgatt ttctatgtac tatagtgtat tattctgcat taagccagat
      481 tttcttttcg taaaaaagta ataattttag gaagcaaata attcaggaaa agtacgattg
      541 caataaatgg aattatccaa actactcttg cttgatagcg atttagcaca ttcgataaat
      601 ttgcacaaac ggcagcatta aaaaatacac cagaaaaaca aataactaaa aataaataga
      661 ttttatcatc tattttaaat cgatataaaa cacatgctat tagcagtatt gataaaataa
      721 caatgaaata aataacatta tttattgttt cgaattttaa tccttccgtc aattgtttgg
      781 aattatagaa atcccgtttt atttcatttt ttggaaagtt cttataagtg ttgtcataaa
      841 caggatacca ttgttcatta agaaaattta tcatgccgtc tccggttctg aactgaaatg
      901 attgtttaca ggtagagaga gaaaaattcc ataagatagg tccataatat ttgggtgatg
      961 ataaaatatc ttttacaata agttttggtt cttcctgata attattccat ccgcccattt
     1021 tttgaaaaat tcctttggta tcccagatga ataactgaga ccactttggt atatcatctt
     1081 tgtatttaca tatcctgtaa tcttttttat cgcagttttc ttttaaatat gtgtccacaa
     1141 ctccggtgtc aattaatctt gcaaagaaaa aaattccgct tgtagttgaa aggcatggtt
     1201 ttttaaaatg gtcataattg tattttatta tgcatgatat tgtgcctaaa tataccagca
     1261 tgatcattat acttcccaat aatgctttcc ttttgaattc agcaaatttt cttttagaga
     1321 ggataaaaag ccacacggta attaatagca gcgccaaaac cggaatgttg ctcaagtgag
     1381 aaaaaacaat aaaaaatatt aatacggagt aaagaatgta gcttaatgca cagtttttcc
     1441 caaaaagaat attaaagagt gctaagaaca aaaatgaagt taatatgtcg ggcattagca
     1501 tgctgacaaa ccatcccaat gtagaaaaaa gtgaaagaaa acaaatagta ataaagtgat
     1561 ataacttgtt gaaagtaatt tttatcaata ctgtttttaa ggcatggtag attaataagg
     1621 atgcaatcaa tccctgtgaa aatattacca tccacaatgt tgattgccag gtggtagctc
     1681 ttataaaata accatatcct atgggcctgt caacaagagg agttaattta attccttgtg
     1741 caacatataa agcactgtcg caatcaaata aaagataacc attgtataat gccggaatgc
     1801 ataatataat tgctgttgct gcaaaataaa tgatagaata tagtaaggaa gtaactttca
     1861 ttgattaatg caccaaatta ataggttgta aatttattaa ttattttcac taatgaccga
     1921 caaaaaaatg gggttacttc ccgggagttt cacccctgga tgtaagtttg ttttgcgaac
     1981 caaacaaaac aacattggta aagatagcga aataaaactg ggcggacaaa ttgagaacat
     2041 acgcatacag ggaacagggt agtactgtaa tatttgaaac tatttcagat ttatggggtg
     2101 caaagggtta actctttgcc tggcggtatt tcaaattttt caataaaata aaaatttaat
     2161 aatctatatt tttgtgtaag tttgtaatcg ttaccaatga agatgaaaga agaatttgta
     2221 aataacagtg cggcactttt tgaaaggcgc ttcggtgaaa agcccataag cattgatgaa
     2281 ttgccgtcat caggatcaaa ccgttgttat ataagggtgg tttctgaaaa taataaagcg
     2341 ttggcagcat acaacaccga ccggaaagaa aatatcgcct acctgagctt tgccaggcat
     2401 tttgaaaaac acaagctgcc ggtaacaaaa atatttgcag aagatctgga caatcacctg
     2461 tatctgctcg aagaccttgg cgatgagacg ctgtttgcct acctcgaaaa gcatcgcgcc
     2521 gagggctttt cgaacacaac agtgggctta tacaaagagg tgcttgataa tttgctgcgt
     2581 tttcagattg acgccgccgc cgacctggat tattcggttt gttaccctcg ggctgccttc
     2641 gacaggcagt ccatgatgtg ggatctgaac tatttcaaat attatttttt gaaactggct
     2701 cagataagtt tcgacgaaca aaaactggaa gatgatttta atgtttttgt tgattacctg
     2761 ctgcaagccg atgcagatta ttttatgtac cgcgattttc agtcgcgcaa tatcatgatc
     2821 agggatggca aattgtattt tattgatttt cagggtggcc gtaaaggcgc tttgcaatac
     2881 gatgtggctt cgttgcttta cgatgccaag gcggccattc ctcagcaggt gcgcgatgaa
     2941 ttgctggact attatatagc caggctttcg cagaaaaaac aaattgagga atctgaattc
     3001 cgaaggcagt tcgccggctt tgtgctggta cgtatcatgc aggctatggg agcttatggc
     3061 ttccgcgggt attatgaaaa aaagacgcat tttttgaaaa gtattcccta tgcggtaaac
     3121 aacctgaaat ggattttgaa aaatgccgga ttgcctgtaa aaacaagtat tttgcaggat
     3181 gtctttgaac agatcattgc cagcaaaaac ctgcagcgtt atgttgatga aaaaacaccg
     3241 ggattgacgg tgaggataaa tagtttttct tttaaaaagg gttatcccga agatcccggg
     3301 ggtaatggcg ggggctttgt gttcgactgc agggctttgc cgaaccccgg caggtacgat
     3361 gaatacaagg gcttgaccgg aatggataaa cccgtaattg acttccttga acatgaacct
     3421 gaaactgccg ttttttttga aaataccagt cagcttgttt cgcaatctgt ggaaaactat
     3481 atgaaacgcg gctttacgca tcttatggtg agctacggat gcaccggcgg acaacaccgt
     3541 tcggtatatt ttgccgaaaa gctggcagct tatgtcagag aaaagtttga tgcagcgata
     3601 atagtaaatc ataaggaaca aaacacaaag ccatgaaagc aatgatactg gccgctggca
     3661 tgggcacccg cctgggagcc ctgaccgcag aaaaacccaa agcgctcatt gaagtaaacg
     3721 gcaggacact gctggaacat gtggttgaaa acctcaagag gtttggcgta aaggaaatca
     3781 ttatcaatgt acatcatttt ggaggccaga ttattgactt tttgaaacag aaaaaaaact
     3841 tcgacatacg gattgaaatt tccgatgaga gcgatgccct gcttgatacc ggcggcggcc
     3901 tgatgaaagc atcctacttc ttcgacgacg gcaagccgtt tctggtgcat aatgtagatg
     3961 tgctttcgca tatcgacctt cataaactgg aaatctatca tacgcacatc cgctccctgg
     4021 ctaccctggt ggtaaaacac cgcgaaacct cacggtattt cctggtggac aaataccagt
     4081 tgctgtgcgg atggaaaaat aaaaaaaccg gcgaaaaaat ggtggtacgc tatggcgaaa
     4141 aactcaccga agcggctttc agcggcatac agatgctcag ccctgaaata ttcaggttgt
     4201 gtaacaagaa aggggccttt tcactgaccg aaatgtatct cgacctggcg gccgaaaaca
     4261 aaattgtggc ctataacgac ggctccctgt ggtttgattt gggaaaaccc gaaaatattg
     4321 ctgaagcggc aaaacacttc gtataatgcc gttaattctg cgaacgcgat aacatgattt
     4381 tatatttatg aaaatagcgg tggtgggagg tggcgcggcc ggcttttttt cggcgctgac
     4441 agctaaagag aactttccgc aggcacaggt atatatcttt gaaaaagccc gtcggttgct
     4501 gtcaaaagtg aggatctcgg gcggtggccg gtgcaatgtt acgcatgcat gccccacggt
     4561 ggaagacttg tgtgcggcct acccgcgtgg cggcaggaag ctgaaaaagg cgtttcatat
     4621 ttttaacaat acccacacct gcgcctggtt cgaagcgcgt ggtgtgccac tggtcgttca
     4681 ggacgacaat tgtgtgttcc cggcttcgca aaattcacaa agcatcatcg tctgctttat
     4741 gcgtgaggcc gaaaggctgg agatacacat cagcggcggg gtgggggtta ctgcccttac
     4801 tgccggcgac aaggggctcg aactttcttt tgatgtgccc ggccgccagc ctgaggtttt
     4861 tgataaagtg attgtagcgt caggggggtc gcccaagcag gaaggcctgc aatggctcga
     4921 agcgctcggg cataaaattg aaacgcccgt accgtcactg tttactttta atatgcccgg
     4981 cgaatcggtt acaaagctga tgggcgtagt ggtgccggat gttgtggccg gtatccaggg
     5041 aaccaaattg cgggctgcgg ggcctctgct cataacacac tggggcatga gtgggccggc
     5101 tatcattaat ttatcctctt taggcgccag actattaaac gagaaaaact atagttttgt
     5161 ggtacagcta aactgggtaa accaacaaaa tacgctcgct gttgctgaaa atcttcaacg
     5221 tatcgcctcg gctcatgcgg ccagggcaat acacaatttc aggccctacg acctgccttc
     5281 gcgcttgtgg gaatacctgt tggcaaaatg cggcatggcc cacgacaaaa cctgggaaga
     5341 attaggaaaa aaaggcctca ataagatagt caatgtatta accaacgaca cctatcaggt
     5401 gcagggacaa accacgttca gagaggagtt tgttacctgc ggtggtgtga gtcttgacag
     5461 cattgattta aacaccatgc agagcaaggt atgccgaaat ctttattttg ccggcgaggt
     5521 gctcgatatt gacggaatta ccggcggcta taattttcaa tgcgcctgga ccacgggttt
     5581 tattgccggt aagttgcaat aaaaattttg ggat
//
LOCUS       DAOSFP010000131         5608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12788_length_5608_cov_4.362327, whole genome shotgun sequence.
ACCESSION   DAOSFP010000131 DAOSFP010000000
VERSION     DAOSFP010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12788_length_5608_cov_4.362327"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /locus_tag="PLI16_05055"
     CDS             <1..841
                     /locus_tag="PLI16_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH83961.1"
                     /translation="YYSGNWYFPNSSVNDIGFIHALVDSARASYSIDPSRMYICGFSM
                     GGFMTERMALQSNLDFAAFASMSGTIGAGISTYNPGRSVPIAHFHGTADSTVYYVGNQ
                     FGIDPDSLIRFWVVNNGCNPVPDSTGFADTAADSITVDKFSFTGAGPDNEVLFYRMNG
                     AGHEVLYEPDNDMTEILEIWKFFRRHQNATASVAEQVFQNDIFVYPNPAADFINIILP
                     ETVGKTTVELFTLQGTLVYSATPAGQFHHIPLQSSVFRNGMYLLRVSGPGVNLARRLV
                     VQH"
     gene            1004..3832
                     /locus_tag="PLI16_05060"
     CDS             1004..3832
                     /locus_tag="PLI16_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH83962.1"
                     /translation="MNYIKKIVLLFLLLGAGTLYAQTPGRVTIKGVIKDKDNNEAAFA
                     TVMLLSPKDSTLQNFTQSNEHGGFAFNNVRNTAYLLKVSHISFLPLQQYLAPANTSIN
                     DLGTVSIKPIAKELFEVVIREAKAPLSIRGDTIEYDATTFRAPPGSSVEDLLRRLPGI
                     EVDASGNLKSQGKDVKRVYVDGKTFFGDDPAGATKNLDANAVSKVQVFDEKSEQAQLT
                     GVDDGVKEKAMNLELKDEFKKGNFGKVTVAGGANEEGVLRWAGRGNFNRFDKKSQLSF
                     IGYANNINETGVNWEDYSEFKGQSTFDRYDNGDFGFSSGRGRYFYMTEDDSPISYFDG
                     RGFTKNYGGGANYNYDNKITKFNVSYFYKNNQQEYDQFDYKETFFQDSSYFNNDTTDY
                     SDTRQSHTVAARIEQNIDSNNVLIVKANFRYNITGRNNLKTSFYSDGANEIINNLLTN
                     NASDINSWKLNSTAIYRHKFKTKGRSFAVSAAYNINPGNENEKYYSRNIRFIPSYEEV
                     LHRYSDRDYNKQQIKSSLLYTEPLSKKFFMEVFYNFNTSSNKVNRQITDAIHSEIRID
                     SLSVYYTNKVMINRLGTDFRYSYNGLNIMLGVAGQYLTLNGKYAVDENMPLITDPIEK
                     DYWSLCPKFNISYEFPNNMWLEFDYGYDVTEPSFDYLQPVPSVSNPLYRTVGNINLKP
                     ERTHNFELDYNYYNPATFSHAGISAQYSYCANKISYNQNMVWLDSLGIVTVSAPENNS
                     ESHSINSWAWFGYPIIKTKFTNDWSVGFNYDNYGSFVNEVFNQNHQYRYNLNTSFSIT
                     PIQQLVIALSGRASLADARLSMNSERNQFSQTYKAGLSVKWQFLTKTFFESNFDYEFY
                     RNSLYGYEKNIPVLNASIRQLIGKKNHFEVRVAAFDIFNQRQYVQQFSYQNYYGRSVA
                     ETLARYFMLSVSYNIKGYETKIKKDRHF"
     gene            3855..4607
                     /locus_tag="PLI16_05065"
     CDS             3855..4607
                     /locus_tag="PLI16_05065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HOH83963.1"
                     /translation="MKKILLAIAFLAFFGMCFKSSGQQAGKIKYLTTYNWVKMLNACD
                     YLSKESKERTAYMWGNRSEWKIYSILYFSDTQSRFEDSKESPDGDRGGYQGRKETFFM
                     IHNFKDQTYVHALEMLGKTYLISDSLKMPDWKIKNDLKEVAGHVCMNATLKDTLRGQK
                     IVAWFALDMPVQAGPDRFFGLPGMILEVDINNGAEVLTAEKIENVSLTNEFELPKKLK
                     AKKLNGTEYMAILKNFYEDKRKAEEFPWGVRY"
     gene            complement(4806..5504)
                     /locus_tag="PLI16_05070"
     CDS             complement(4806..5504)
                     /locus_tag="PLI16_05070"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH83964.1"
                     /translation="MSFDIASQILYEDNHLIIINKKPSQLVQPDLSGDDSLELAIKLF
                     LKQKYHKPGEAFLGVVHRIDRPVSGTVVFAKTSKALVRLNEMLKKHELKKTYWAIVKN
                     KPPQDAGVLSQYLLRNPKQNKSVVYHVPVENAQLAELCYRMIGNSDQYFLLEIDLMTG
                     RHHQIRAQLAALGCPIKGDLKYGFDRSNRDGSICLHAVKIDFIHPVSRERVSVQAPLP
                     EGDVWKFFSAYALP"
     gene            complement(5507..>5608)
                     /locus_tag="PLI16_05075"
     CDS             complement(5507..>5608)
                     /locus_tag="PLI16_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOH83965.1"
                     /translation="RRYHNLQEEVLGAVTAYIKDVKSRDFPNEKEQY"
BASE COUNT         1617 a         1203 c         1238 g         1550 t
ORIGIN      
        1 gtattatagc ggcaattggt attttccaaa ttcaagtgtc aacgacatag gttttatcca
       61 tgccctggtt gattcggccc gtgccagtta ttctattgat ccttcacgca tgtatatctg
      121 tggattctcg atgggaggat ttatgaccga gcgcatggcc ttgcagtcaa accttgattt
      181 tgcagctttt gcttcaatgt cgggaacgat aggagcaggc attagtacct ataatcccgg
      241 gcgatcggtt cctattgcac attttcatgg tactgctgat tctaccgttt attatgtcgg
      301 taatcagttt ggcatagatc ccgattcatt aattcgtttc tgggttgtga acaatggctg
      361 caatccggtt cctgattcta ccgggtttgc cgatacggcc gccgacagta tcacggtgga
      421 taaattttcc tttaccggcg ccggccctga caatgaggtg ctgttttacc ggatgaacgg
      481 cgccggccat gaagtgttat atgaaccgga taatgatatg accgaaatac ttgaaatctg
      541 gaagtttttc agacggcatc aaaatgctac ggcctcggtt gccgagcagg tttttcaaaa
      601 cgatattttt gtgtatccca atcctgctgc tgattttatc aatatcatcc tccccgagac
      661 tgttgggaaa acaaccgtgg aactgtttac actgcaaggg acactggtat attccgcaac
      721 accggccgga cagtttcatc atatcccatt gcaatccagt gtgtttcgca acggcatgta
      781 cctgctcagg gtgagcggtc ccggcgtcaa tcttgcccgc cgcctggttg ttcagcatta
      841 aaagggttgt tatctttttg aaatccaggg tgtttaataa tattctggat ttttttttga
      901 tatgaaaaat aattaatttg gatttttata gttaaaatta tggccggata tgtaacaaaa
      961 ataaatttcg gaagtcctat tgtgcgatca tataaatact aaaatgaact acattaaaaa
     1021 aattgtctta ctgtttttgt tactgggtgc tggaaccctt tatgcccaga caccgggacg
     1081 cgttaccatc aagggtgtga taaaagataa agataataat gaggctgctt ttgctacggt
     1141 aatgttgctt agcccaaaag actccacgct gcaaaatttt actcaaagca acgaacatgg
     1201 cggatttgca tttaataatg taagaaatac ggcctacctg ctgaaagttt ctcatatcag
     1261 ttttttgccg ctgcagcaat acctggcgcc tgccaatacc agcatcaacg acctgggtac
     1321 cgtatccatc aaacctatcg ccaaggagct ttttgaggtt gttatacgcg aagccaaagc
     1381 ccctttatct atcagaggcg atacgatcga atacgatgcc accaccttca gagcgcctcc
     1441 gggctcttcc gttgaagacc tgttgcggcg gctgcccggt atcgaagtgg atgcttcggg
     1501 gaatctgaaa tcgcagggca aagacgtaaa aagggtatat gtggacggga aaactttttt
     1561 tggtgatgac cctgcaggag caacaaaaaa tctcgatgcc aatgcggtgt ccaaggtgca
     1621 ggtgtttgac gaaaagtcgg aacaggcaca gctcaccggt gtggatgacg gtgtgaaaga
     1681 aaaagccatg aacctcgagt tgaaagatga gtttaaaaaa ggtaatttcg gtaaagttac
     1741 tgtagccggc ggcgccaatg aagaaggggt actacggtgg gcaggacgtg gcaattttaa
     1801 ccgttttgat aaaaaatcac aactatcgtt tattggctat gccaacaata taaatgaaac
     1861 cggtgtcaat tgggaagact atagtgagtt taagggacaa tccaccttcg accgctacga
     1921 taacggcgat tttggattca gctcaggcag aggaagatat ttttatatga ctgaggacga
     1981 ctctcccatt agctatttcg acggacgtgg attcactaaa aactatggcg gcggagcaaa
     2041 ttataactac gataataaaa tcaccaagtt caatgtctct tatttttata aaaataacca
     2101 acaggagtac gaccagtttg actataagga aactttcttt caggatagct cctattttaa
     2161 taacgacacc acggattatt ccgacacaag acaaagtcac accgttgcgg ctcgtatcga
     2221 acagaatatc gattccaaca atgtgttgat tgtcaaggct aatttcaggt acaatatcac
     2281 aggcaggaac aacctgaaga caagttttta ttccgatggc gctaatgaga ttatcaacaa
     2341 cctgcttacc aataatgcct cggatatcaa ttcctggaaa cttaacagta ccgccatcta
     2401 cagacataag ttcaaaacaa aaggacgttc ctttgccgtt agtgccgcct ataacattaa
     2461 ccccggcaac gaaaatgaaa aatactattc ccgcaacatt cgcttcatcc cttcttacga
     2521 ggaagtgctg catcgatact ctgacagaga ttacaacaaa caacagataa aatccagcct
     2581 gctgtacact gagccgctgt cgaagaagtt ttttatggaa gtgttctaca actttaacac
     2641 ctcaagcaat aaggtaaacc gccagattac ggacgccata cacagcgaaa tacggatcga
     2701 cagcctgagc gtttattata ccaacaaagt gatgatcaac cgcctgggaa ctgattttcg
     2761 ctattcctac aacgggctga atatcatgct gggcgttgcc ggacaatacc tgacactgaa
     2821 cgggaagtat gccgtggacg aaaacatgcc attgattacc gaccctattg aaaaagatta
     2881 ttggagcctg tgccccaaat tcaatatttc atatgaattt ccaaacaata tgtggcttga
     2941 gtttgattac gggtatgatg taaccgagcc ttcgtttgat tatttgcagc cggtacccag
     3001 cgtatccaat cctctttatc gcacggtggg caatatcaac ctgaaacccg aacgtaccca
     3061 caattttgag ttggattaca attattataa tccggctact ttctcgcatg ccggtatcag
     3121 cgcacaatac agttattgcg ccaacaaaat ttcgtataat caaaacatgg tgtggctcga
     3181 ttcgctggga atagtaaccg tatctgcacc ggagaataat tcggagtcac atagtattaa
     3241 ttcctgggca tggtttggtt atccgataat aaaaacaaaa tttaccaacg actggagtgt
     3301 gggcttcaat tatgataact atggctcttt tgttaatgag gttttcaacc aaaatcacca
     3361 ataccgttat aatctgaaca cttcgttcag catcaccccc atccagcagc ttgtaattgc
     3421 tttgtcggga agggcttcgc tggccgatgc ccgcctgtcg atgaattcgg aacgcaacca
     3481 gttttcgcag acctataaag ccggattatc agtaaaatgg cagtttttga cgaaaacatt
     3541 ttttgaaagt aattttgatt atgagtttta ccgaaactca ctttatggat acgaaaagaa
     3601 tattcctgtc ctcaacgcct cgatacgtca gcttatcgga aagaaaaacc attttgaagt
     3661 acgggtggcc gcttttgata ttttcaatca acggcaatat gtgcagcaat tttcttatca
     3721 aaactattac gggcgcagtg tggccgaaac gcttgcacga tattttatgc taagcgttag
     3781 ttacaatatc aagggatacg aaacgaaaat aaaaaaagac aggcatttct gataatcaac
     3841 ataatcaata ccgtatgaaa aaaattctct tagcaatagc ttttcttgcc tttttcggaa
     3901 tgtgttttaa aagttccggg cagcaagcag gcaaaataaa atacctcact acctataact
     3961 gggtaaagat gcttaacgcc tgcgactacc tgagcaaaga aagcaaagaa cgaacggctt
     4021 atatgtgggg caaccggtcg gaatggaaga tatatagtat tttatatttt tcggatactc
     4081 aatcacgttt cgaggattct aaggaaagtc ccgatggcga tcgcggcggg taccaaggca
     4141 gaaaagaaac gttttttatg attcataatt ttaaagatca gacttatgtg catgcgttgg
     4201 aaatgcttgg aaaaacttac ctgatctccg attccctgaa aatgcccgac tggaaaatca
     4261 agaacgacct gaaagaggtg gccggccatg tgtgtatgaa tgccacgctg aaggataccc
     4321 ttcgcggaca gaaaatcgtg gcctggtttg ccctggatat gccggtacag gctggccccg
     4381 accggttttt cggattgccc ggaatgatac tcgaagtgga tattaacaat ggtgctgaag
     4441 tactgactgc cgaaaaaatt gaaaatgttt cactgactaa tgagtttgaa ttacccaaaa
     4501 aactcaaagc caaaaaactc aatggcactg agtacatggc tattctcaaa aatttctatg
     4561 aagacaaacg caaggccgaa gaatttccat ggggcgtcag gtattgattt cggtgtgtat
     4621 aacaggtcgg gttgacccca tcaaaggaaa ttcccggaac caccaatcat ttgcggattc
     4681 tccgggtttc ttttggcgga ccgaagggta aagggtattt tacttgaatt tttcggctgg
     4741 gcagaagcag caggcagata tcaaagtgag gaggccggcc gtgttactat accatctgcc
     4801 aactttcacg gcaaggcata agccgaaaaa aatttccaca catcaccttc gggtaatggt
     4861 gcctgtacag atacacgttc ccggcttacc ggatgtataa aatcaatttt cacggcatgc
     4921 aaacatatcg atccatcgcg gttgctgcgg tcaaagccgt atttgaggtc gcctttgata
     4981 ggacaaccta aggccgccaa ttgggcgcgt atctgatggt ggcggccggt catcaggtcg
     5041 atttccagca aaaaatattg atcgctgttg ccaatcatgc ggtaacaaag ctcggccaac
     5101 tgagcatttt caactggtac gtgatatacc acacttttgt tttgtttcgg gtttcttaaa
     5161 agatattgcg aaagtacacc ggcatcctgc ggtggcttgt ttttaacgat agcccaatat
     5221 gtttttttta attcgtgttt tttcaacatc tcgttcaggc gcactagggc tttgctggtt
     5281 tttgcaaaaa ccaccgtgcc gctcaccgga cggtcgatgc ggtgtacaac acccagaaaa
     5341 gcttcaccgg gcttgtggta tttttgtttt aaaaacaatt ttattgccaa ctccagcgaa
     5401 tcatcgcccg acaggtcagg ctgaaccaat tgtgatggtt ttttattgat gattatcagg
     5461 tggttgtctt catacaaaat ttgcgaggca atatcaaacg acatggttaa tattgctctt
     5521 tttcgtttgg gaaatctctt gatttcacat ccttgatata cgccgtaacg gctccaagca
     5581 cttcttcctg gaggttatgg tagcgacg
//
LOCUS       DAOSFP010000132         5584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12863_length_5584_cov_4.648399, whole genome shotgun sequence.
ACCESSION   DAOSFP010000132 DAOSFP010000000
VERSION     DAOSFP010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12863_length_5584_cov_4.648399"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1009
                     /locus_tag="PLI16_05080"
     CDS             113..1009
                     /locus_tag="PLI16_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83966.1"
                     /translation="MFLGYNYIDVAINVVLMLIMLGIGLSLTIKDFKNIFVHPKSLAI
                     ALTVQLFVVPLVAFGIAYISNVPAEAKVGIVIIALCASGASSNLITHLFRGNVALAIS
                     MTTINSFITLVSIPLYANLALNVFLGFHTEIHLPVWETIMQIFIITIIPAGIGVFIRH
                     QKESLAKSLERPLKFILPAMLGFIFTVKIFVSETSGGTGITLTETLQLLPYLLLLNFL
                     AMALGFFVARKSRLPFADQYTISIEVGLHNTALALLISGTILNITAMEKPAIVYAMFS
                     FFTAIIFVLAIKKIFNNKIRKT"
     gene            1147..3486
                     /locus_tag="PLI16_05085"
     CDS             1147..3486
                     /locus_tag="PLI16_05085"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOH83967.1"
                     /translation="MRKILFLILFFLTFSVNAQEFLPFASGSYAGITGVHLQPASIVD
                     SRYKLDIGLSSTSLGLSNNYVSMDTKPLFNRDEFGNPDFADLYLKYNNNGKPKSAFIS
                     LRQDVLSFMISITEKDAIAFTPSVRAMINMDGVSQDLLDLIQANWDVPSLFNQQLNNA
                     RLSVQANAWAEYGLTYARVVYDNEEHFIKAGVTLKLLQGLGSGYLFARNIDYSMKNKD
                     TMSIFNSEVNYGVTDNLTKRDNGKNDYQVNSKFTVGMDFGAVYEYRPKWMDYKYDMDG
                     KTNLWRRDQNKYLVKVGISLTDVGRVRYNRGPQTKDFRAHVSEMDIREIGIYDYNDFN
                     DFIDSTFTFYDVTDKYTMNLPMSLSLQADVRVVKGFYINFVPYLALMQGKNDENKNHY
                     LSSINLIPRYDIAWFGASLPMQYNSNNKFNVGIGLRLGPVWIGSNDIFSTLIGGKVSR
                     ASVCAALKIPILYSAPHDRDKDNVSDKLDQCVDLPGTWELKGCPDRDGDGIVDNKDKC
                     PDVPGLPMYDGCPDRDADGIIDMNDDCPDDKGLEKYKGCPDTDGDGIIDKEDDCPLVA
                     GIPAFKGCPDTDGDGLADKDDNCPTVPGTIENKGCPFIDSDGDGIADKDDDCPSIPGE
                     KAFSGCPDTDGDGISDKYDMCPTIKGIPENKGCPEIKKEEQETIRKAFDNLEFETGKA
                     IIRTSSYPSLNELADVLKKREDFKLLISGHTDDVGNDASNMTLSKNRTLAVRDYLVGK
                     GIDASRFKTEWFGETRPLVPNTSPENRQKNRRVEMNIFF"
     gene            4082..5293
                     /locus_tag="PLI16_05090"
     CDS             4082..5293
                     /locus_tag="PLI16_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOH83968.1"
                     /translation="MAEIVEENVEREKMEFEKEEPSHDSCGKEEPLAINRMKPTLVII
                     EVEDYRKIILRFPFAKEHIAKIKTLPYYYWNKNERYWSFPYTENIIAELESYFKKFNY
                     QIETRHTSKKSSGGKIAKKYGNERKCPEEYIEKLKLKRYSDNTIRTYTGAFADFINYF
                     SEKDLDEISENDIKSYLLYLFEKRKISTSYQNQVINAVKFYYEKVCNGKKLPYIKIDR
                     PFKEKYLPTVLSEDEVKRIINCVSNLKHKAILLTIYSAGLRISELINLRIADIDSDRK
                     AIVIKGAKGKKDRNSLLSEKLLLVLREYFKKYRPKMWLFEGQYGEQYSVTSVQHVFRR
                     ACIDANIMKKASVHTLRHSFATHLLERGVDLRYIQELLGHSSSKTTEIYTHITHRGME
                     QIKSPLDNMDI"
BASE COUNT         1730 a         1041 c         1245 g         1568 t
ORIGIN      
        1 atgtcggggt tacccagatg cagcgcacga tgccacccac tgcgcaatag gaacgtagtg
       61 aactattgcg caatctggat taaatattaa tatctttgcg taatctttca atatgttttt
      121 aggatacaat tacatcgatg tggcgattaa tgtggtgctg atgctcatca tgctcggcat
      181 aggcctgtcg ctgactataa aagattttaa aaacatcttt gtgcatccca aatcacttgc
      241 tattgcgctt acggtgcagc tatttgttgt tccgttggtg gcttttggta ttgcctatat
      301 atcaaatgtg ccggccgaag ccaaagtagg tatcgtcatc attgccttgt gtgccagcgg
      361 tgcctcgtca aaccttatca cccatttgtt caggggtaat gtagccctgg ccatttccat
      421 gacaaccatc aacagcttta tcaccctggt gagcatcccg ctttatgcca acctggccct
      481 caatgtgttt ttgggttttc atacggaaat acatcttccg gtatgggaaa caatcatgca
      541 aatttttatt atcaccatca tcccggccgg cattggcgtt tttattcgcc accaaaaaga
      601 atctctggct aaaagtctcg aacggccgct aaaatttatt ttgccagcca tgctgggttt
      661 tatttttacc gtaaaaatat ttgtcagcga aacaagcggt ggaacgggca ttaccctgac
      721 cgaaaccctg caactattgc cctatctgtt gttattgaat tttctggcga tggctctcgg
      781 tttttttgtg gccagaaaaa gcaggctgcc ttttgccgat caatacacca tttcaattga
      841 ggtgggcctg cataatactg cgctggccct gttgatttcg ggaactatac tgaatattac
      901 cgctatggaa aaacctgcta tcgtatatgc tatgttttcg ttttttacgg ctataatttt
      961 tgtgttggcc atcaagaaga ttttcaataa taagatcagg aaaacgtaac ttgcaacctg
     1021 tttacatgat attgcgtata aagttatgca aataagacaa tatctgataa tgaaatactg
     1081 gtggtagttt atatttcttt gctttagata ataaaaggca tttttattca tttaaaataa
     1141 ttaattatga gaaaaatttt attccttatt ttgtttttcc tcacgttttc ggtcaatgcg
     1201 caggaatttc ttccttttgc atcgggatca tatgcgggta ttaccggggt acacctgcag
     1261 ccggcatcta tagttgactc caggtataag ctggatattg gcctgtcttc aaccagtttg
     1321 ggcttgtcca ataattatgt atctatggat acaaagccat tatttaaccg ggacgagttt
     1381 ggaaacccgg acttcgccga tctttatctg aaatacaata acaacgggaa acccaagtcg
     1441 gcttttattt cgttgaggca ggacgtgttg agtttcatga taagcattac cgaaaaagat
     1501 gctattgcct ttacaccttc ggtgcgtgcc atgattaata tggatggggt ttcgcaggat
     1561 ctgttggatc ttatccaggc aaactgggat gttccatcat tgtttaatca acaattgaac
     1621 aatgccaggc tgagcgtaca agccaacgcc tgggccgaat acgggcttac atacgcacgc
     1681 gtggtatatg ataatgaaga acattttatc aaagcgggtg ttacactaaa acttttacag
     1741 ggattaggct cagggtacct gtttgcccga aatattgatt attcgatgaa aaataaagac
     1801 accatgtcaa tatttaattc ggaagtgaat tatggtgtta ctgataatct taccaaaagg
     1861 gataacggga aaaacgatta tcaggttaac tcaaaattta cggtgggcat ggactttggc
     1921 gctgtttacg aataccggcc taaatggatg gactataaat atgatatgga tggcaaaacc
     1981 aatctgtggc gccgcgatca gaataaatac ctggtaaaag taggaatttc tcttaccgat
     2041 gtgggtagag tcagatataa cagaggcccg cagacaaaag attttcgtgc gcatgtgtcc
     2101 gaaatggaca tcagagaaat cgggatttat gattataatg atttcaatga ttttattgat
     2161 tctactttta ctttttatga tgtaacggat aaatacacca tgaatctccc catgagttta
     2221 agtcttcagg cagatgtcag ggtggttaaa gggttttata taaattttgt gccttatctg
     2281 gcgctgatgc agggcaaaaa tgacgaaaac aaaaaccatt accttagttc aataaacctc
     2341 attccccgtt atgatattgc ctggtttggg gcttcgctgc ctatgcagta taacagcaat
     2401 aataagttca atgtaggaat tggcttacgt ttggggccgg tatggatagg gtccaacgat
     2461 attttctcca ccctgatagg gggaaaggta agcagggcgt cggtttgtgc tgcgcttaag
     2521 atccccattc tttatagcgc gcctcacgac agggacaaag ataatgtatc agacaaactg
     2581 gatcagtgtg tagatctccc cggaacatgg gaacttaagg gctgtcctga ccgcgatggc
     2641 gatggcattg tggacaataa agacaaatgt cctgatgtgc ccggcctgcc tatgtacgat
     2701 ggctgtccag accgtgatgc cgatggtatc attgatatga acgatgattg tccggatgat
     2761 aagggccttg aaaaatacaa gggttgtccc gataccgacg gcgacggcat cattgataaa
     2821 gaagacgatt gtcccctggt tgccggtatt cctgccttta aaggctgtcc cgataccgat
     2881 ggcgacggcc tggccgacaa ggatgataat tgtcctaccg ttccgggaac tatcgaaaac
     2941 aaaggttgtc cgtttatcga ttccgatggc gacggcatag ccgacaaaga cgacgattgt
     3001 ccgtctatcc ccggcgaaaa ggcatttagc ggctgccccg ataccgatgg cgacggaata
     3061 agcgataaat atgatatgtg cccaaccatt aaaggtatcc ccgaaaacaa aggctgtccg
     3121 gaaatcaaga aagaggaaca ggaaaccata cgtaaggctt tcgacaacct ggagtttgaa
     3181 acaggcaagg ccataatccg caccagttct tatccttcgc tcaacgagct ggccgatgtt
     3241 ttgaagaaac gtgaagattt taaactgctt atttcgggcc atactgatga tgtgggcaac
     3301 gatgccagca atatgacctt gtcgaaaaac cgtacactgg ctgtgcgtga ttacctggta
     3361 ggcaaaggga ttgacgcttc cagatttaag acggaatggt ttggcgaaac caggccgctg
     3421 gtgccgaata cttcccccga gaaccgccag aaaaaccgca gggttgagat gaatattttc
     3481 ttttagtaat tagtgtttgt aagaaaacgc caattttttc gttacgcttg caacaaaaat
     3541 gctcatttac attagtaaac tctgcttttt gtcgcttcgc aagctcgcct gactggcgca
     3601 gtcgggcagg cctcaaactt gacgttttct ttgtacaaac acgatttttc ttataaactc
     3661 taatcaggca tataaaagta tcccggattg acggaaagtt ggtccgggat ttttttgtat
     3721 cggtggcttc gagggcaatc atgatatcgg tggcttcgag agcctcagcc accgagggag
     3781 ggcattttga caagctcaat gtgtcacctc ggccaccggg gagaaaaaaa tattaaaaat
     3841 ggttctgttg atatgaaaat attttttatg tttgtatggc gtattgagta tgaagtctgt
     3901 catattaaaa ccggttaatc atcgcgggga ggagcggttg cttgttcagt ttccttacga
     3961 tatggagctt atcgcttgta tcaggaaggt taagggcgct acgttcagct caagctataa
     4021 gagctggcat gtgcccaaca gcaacgaaag cattgaggag ctgaagaggg tttttgccgg
     4081 agtggccgaa attgttgagg aaaatgtgga acgagagaaa atggagtttg aaaaggaaga
     4141 gccgtcccat gattcttgcg gcaaagagga acctctagct ataaacagga tgaagcccac
     4201 cctggtaatc attgaggtgg aggattacag aaaaataatc cttaggtttc cttttgcaaa
     4261 ggaacatata gcaaaaataa aaaccctgcc ttattattat tggaacaaaa atgaaagata
     4321 ctggagtttt ccttacacag aaaacattat cgctgaactt gagagctatt ttaaaaagtt
     4381 taattatcag attgagacca ggcacacaag taaaaaaagc agtggtggaa aaatagccaa
     4441 aaaatatggc aacgagcgta aatgccctga ggaatacatt gaaaaactaa aattaaaaag
     4501 gtacagtgat aatactatca ggacttatac aggggctttt gcagatttca tcaactattt
     4561 cagtgaaaag gacctggatg aaataagtga aaacgacatt aaaagttatc ttttatatct
     4621 ttttgaaaag cgtaaaatct ccacttccta tcagaatcag gtaataaatg ccgtaaagtt
     4681 ttattatgaa aaagtatgca atggaaaaaa attgccctat ataaaaatag acaggccctt
     4741 caaggagaaa tatttaccaa cggtattgag cgaggatgag gtaaaacgaa ttataaattg
     4801 cgtttctaac ctgaagcaca aagccatttt gcttactatt tattcggcag ggctgcggat
     4861 aagtgaacta ataaacctta gaatagcaga tatcgacagt gatagaaaag ccatagtaat
     4921 aaaaggggcg aaaggaaaga aagacagaaa tagtttgttg tcggaaaaat tattattagt
     4981 tttgagagaa tattttaaga aataccgtcc caagatgtgg ttatttgaag ggcaatatgg
     5041 tgaacaatac tcagttacca gcgtacagca tgtatttaga agggcctgta tagatgctaa
     5101 tattatgaaa aaagccagcg tacatacatt aagacacagt tttgctactc atttgcttga
     5161 aaggggagtt gatttaaggt atattcaaga attacttggg catagttctt caaaaactac
     5221 agaaatttac acacatatta cgcatagggg aatggaacaa ataaaaagtc ctttagacaa
     5281 catggatatt taaaaggtaa ataattgata tttaatacta taaaagcaca aaatcagatt
     5341 gctgccacaa ataagataat tttgaataaa aaaattcgga ggaatggtgt atctgagata
     5401 ttggagtcgg acaaaattat gaaataaaaa acgcaagtta ttaataataa atgttataca
     5461 aataaaaaaa tggattgttg ccacaaaata gataaggtaa atatagcagt acaaaggcag
     5521 cagcacaatc gttacaccca attgcaagag acggaaaaaa agattgaatt taaacctgct
     5581 gacc
//
LOCUS       DAOSFP010000133         5583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12868_length_5583_cov_3.611975, whole genome shotgun sequence.
ACCESSION   DAOSFP010000133 DAOSFP010000000
VERSION     DAOSFP010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12868_length_5583_cov_3.611975"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="PLI16_05095"
     CDS             <1..1995
                     /locus_tag="PLI16_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOH83969.1"
                     /translation="GKITFSWTDAEFSGQDLADGEALFSLHFLLTGSAGQHSVITFVD
                     TPTPVEVSDSSLNILPAGTIGGDIAITGASQVFELKIDSVNGSTGFLVSVPVRVWQFN
                     KIISLQGTVTFDPSHAVFISTEQYGLPGMDSTNFGTMLANQGKLMFMWTDATASGQNL
                     NDSSVLFAIKFLVTASLGSSPYLDFSNAPTPIEITDTSQGEISFNTHYGKISVLGGGY
                     KLSGKTKYGARAYAGNPVPSVPTYSSLKYNISQVIVKLKSYPGGLELARDTSNTWGTY
                     GFNNVPDGTYMLSYDKYTADTMQMGNDINAIDVAFLKYLVGHDTLQDPSRNFSRRHKK
                     AADVDNNTLFNALDIARLKAKVGQPYSATANFSKGNWPAADTLVTIAGHDLIVNLTTI
                     GYGDYDASSSKYKDSANNWNMAKSSATANIIYQSTEILTVNKGAYFEIPLKILSKIND
                     FSAMGLELNYPATYFKLVSASMPKGNKKTFTKINPSFEEIVAEDNDLLVTDADGIIRV
                     VYATTGFYDVAAGDEILVLGFKPLQEMPQGDTEFYLDGTGIMADQYGRENENTGLSMP
                     EIFFQGNYEEKNLEFSGFPNPMTGHTVLTFETPENASVKLSLYNARGEKIDELLNKSL
                     ADGKHTMPFFCSQLPSGLYLFTLEITGSTHSYRLTLKMTQ"
     gene            2238..3416
                     /locus_tag="PLI16_05100"
     CDS             2238..3416
                     /locus_tag="PLI16_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83970.1"
                     /translation="MKKPYPNLKKAVKKFVGMRVASLIAILIFAISVVNAQNSTNIPD
                     SEKAKANSGAIQSQTATPPPVPAASLQNMGVSKDKLGAHAPDVNDPDFAAKKAEWIKN
                     YPEEYNALLNPANNGKSAAPNSDQVTKPASGTITNAKKTDASSSATNPNEYAPGYKIA
                     PHAPEFNDPDYEAKKEAWMTNYPEEYNALQQSGNTQTKVINTNSNVTPVNTKVAEHAP
                     ELGDPDYAAKKAEWIKNYPEEYESIGGASTDNGVSNQVKTQVAIPDLQVAKPHTKIAE
                     HAPYLDDPDYEAKKAIWMKNYPEEMNAVLQHSSNTNKNIRPATNTTLEKPATKVAEHA
                     PYLNDPDYQAKKAEWIKNYPQEYNAVNNKPANSVKNSSAGQGVQTPATSVPVYDATRK
                     "
     gene            3469..>5583
                     /locus_tag="PLI16_05105"
     CDS             3469..>5583
                     /locus_tag="PLI16_05105"
                     /inference="COORDINATES: protein motif:HMM:NF016071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HOH83971.1"
                     /translation="MKRFTIFIKKIFVLLAFLALTANSSVWGQACDYFTAANSTTVTG
                     WTEQVGDWQIYNNTLQSPATAEWNYITRDGSTQTNGCVSLRATYTGAAGTKYIGAVGR
                     YSDASNYLMAKIQDNGSAGYWDSYFIYENGSIIANASGLNFGTDVNIALEYNGTAISM
                     KIDTDLNGTWDYTYTATSTTTNSGLCGVGAYNLANVDNWCYGSTCITCAPPANETCAG
                     ATAIYYYNDPYHTSGVLGCTDDCSGTANFDVFYTFTPQYTGSYTADMGLSAGDTYMRI
                     FSGSCCGTLVASDDDSYGDMDPSITLTLTAGTTYWFECGSWSSSGMQNSPYNFNLVRN
                     CTPPANETCSTPTPISYSQIAAGFNTSGTLGCADDCAGYGNDVFFSYDCTCTGSYTFD
                     MRDSDGDTYMRIYSGSCCGTLVASDDDSYGDMDPTITVTLTAGTTYWIECASYTTSSY
                     MSGTYYNLYASTTCILTPGCGNTYLGNITPSSCYPQDANFAASTIPYWSFNATAGTTY
                     NFSLGASAEDTYLHLYNDVMTEVATNDDSGPFYSGSPASLSYTCTTSGTYYITASHWT
                     CNTITTAGYLTYWSSGAPYENTSLQTITPSIAWQNQPYAAGSMDVYRFYGTAGLIYDF
                     SLCSNTEDSYIQIYNSTYDLLADNDDSGPWCTGVAASISFTAPATDYYIVQIRHLGCS
                     GFTNAGDLAYRVQLACTVGCGDT"
BASE COUNT         1678 a         1339 c         1150 g         1416 t
ORIGIN      
        1 ggaaagatta cctttagctg gacagatgct gaattttcag gacaagacct tgccgatggt
       61 gaggcattgt tttcattgca ttttctgctt acaggcagtg ccgggcaaca ctcggttatc
      121 acctttgttg acacacccac tcctgtggaa gtatccgatt cttcgctaaa tatcttacca
      181 gcaggaacta ttggtggtga catcgccatc accggtgctt cacaggtttt tgaattaaaa
      241 atcgattcgg taaatggaag tacaggattt ctggtgtctg ttcctgtgag ggtatggcaa
      301 ttcaacaaga taatttcgct gcagggaacc gtcaccttcg acccatcaca tgctgtcttc
      361 atctcgacag aacaatatgg actgccgggc atggacagta cgaattttgg tacgatgctg
      421 gcaaaccagg gcaaattgat gtttatgtgg accgatgcca ctgccagcgg acaaaacctg
      481 aacgattcgt cggtactgtt tgctataaaa tttctggtta ctgcctctct gggcagttcg
      541 ccttatttag atttcagcaa tgcacccacg ccaatcgaaa ttaccgacac ctcccagggt
      601 gaaataagtt tcaacaccca ctatggaaaa atcagtgtct tgggcggcgg atacaaattg
      661 agcggaaaaa ccaaatacgg cgccagagca tacgccggta atccggtgcc cagtgtgccc
      721 acctacagct ccctgaaata taatatcagc caggtaatag tcaaactgaa aagttatccc
      781 ggaggactgg aattggcgcg ggacaccagc aatacatggg gaacttatgg atttaacaat
      841 gtgcccgatg gcacctacat gctgtcgtat gacaaatata cggctgatac catgcagatg
      901 ggcaacgaca tcaatgctat tgacgtggct tttttaaaat atctggtagg tcacgacacc
      961 ttgcaggatc cgtcaagaaa cttttcgaga agacacaaaa aagccgcgga tgtggataac
     1021 aacaccttgt ttaatgccct ggatattgcc cggttaaagg ctaaagtagg acaaccctat
     1081 tctgcaacag ccaacttttc aaaaggcaac tggcccgctg ccgacaccct ggtaacaatt
     1141 gccggtcacg acctgattgt gaaccttaca accatcggct acggcgacta cgatgcctcc
     1201 agctcaaaat ataaagattc ggcgaacaac tggaatatgg caaaatcgtc ggctacggca
     1261 aatataattt atcagtcaac agaaattttg accgtaaata agggtgccta ctttgagatt
     1321 cctttaaaga tactttcaaa aattaacgat ttttctgcca tggggctgga attaaattat
     1381 cctgcaacct atttcaaact ggtcagtgcg tcaatgccta aagggaacaa aaaaacattc
     1441 accaaaataa acccttcttt cgaagagatt gtggctgaag acaacgacct gcttgtgacg
     1501 gatgccgatg gcataatcag agtggtgtat gccaccaccg ggttttatga tgtagcagcc
     1561 ggtgatgaaa tcctggtgct gggttttaaa ccattgcagg agatgccgca gggagatacg
     1621 gagttttacc tcgatggtac aggaataatg gcagaccaat atggcaggga aaacgaaaac
     1681 accgggctca gtatgcctga aatatttttt cagggaaatt atgaggaaaa gaatctggag
     1741 ttttctggtt ttcccaaccc gatgaccgga cacaccgtat taacctttga aacacccgaa
     1801 aatgccagtg taaaactttc gctttacaac gcccggggcg aaaaaatcga tgaacttttg
     1861 aataaatcct tagcagacgg caaacatacc atgccctttt tctgcagcca gttgccttcc
     1921 ggattatatc tttttacact tgaaatcacg gggtccactc attcataccg cctcacattg
     1981 aaaatgactc aataattatt ataaaaaact atacattaat tagtaagtag tttttttgaa
     2041 tattattcgt cgtattactt taatttttca taattattat ttgggatcat gtcttattcc
     2101 tgcatttgtt aatgaatgaa attgatgtaa attcttctgt tttttttatt aatattgcag
     2161 caaattatct gtttattaat ttgtaatttc taatatcatt aataaattaa ttatcaacta
     2221 ctaataccat taacattatg aaaaaacctt atccaaattt aaaaaaagcg gtaaaaaaat
     2281 ttgttggtat gcgtgttgcc tcgttgattg caattttgat ttttgcaatc tcagtggtaa
     2341 atgctcagaa ttcaacaaac ataccggatt ccgaaaaagc taaggccaac agcggagcca
     2401 ttcagtcaca aacagcaacc cctcctcctg ttccggcagc ctctcttcaa aacatgggtg
     2461 tcagcaaaga caaactcgga gcacatgctc ccgatgtcaa cgaccctgat tttgctgcca
     2521 aaaaagccga atggataaaa aattatcctg aagaatacaa tgcgcttttg aacccggcta
     2581 acaatggaaa atctgctgct cccaacagcg atcaggtaac aaaaccggct tctggcacaa
     2641 tcaccaatgc taaaaaaacc gatgcctcct ccagtgctac caaccccaat gaatacgcac
     2701 ccggatacaa aattgcaccc catgcacccg aattcaacga tcccgattat gaagccaaga
     2761 aggaagcctg gatgaccaat tatcccgaag aatacaatgc ccttcagcaa tcggggaaca
     2821 ctcaaaccaa ggttattaac acaaacagca atgttacccc tgtaaatacc aaagttgcag
     2881 aacatgcacc cgaactgggc gatcctgatt atgctgcaaa aaaagccgaa tggataaaaa
     2941 actatcccga agaatatgaa tcaataggcg gtgccagcac tgataacggc gtatccaacc
     3001 aggtaaaaac acaggttgct attcctgacc tgcaggtggc aaaaccgcac accaaaatag
     3061 ctgaacacgc cccttatctc gatgatcccg attacgaagc caaaaaagct atctggatga
     3121 aaaactatcc cgaagaaatg aacgctgtgt tgcaacattc ctcgaacact aataaaaata
     3181 ttcggcctgc caccaacaca acgcttgaga aacctgccac caaggtggcc gaacacgccc
     3241 cctacctgaa cgatcctgat tatcaagcaa aaaaagccga atggataaaa aattatcctc
     3301 aggaatacaa tgccgtaaac aacaaaccgg cgaactccgt gaaaaattct tcagcaggtc
     3361 aaggtgtcca aactccggcc acatccgttc ctgtttatga tgctacacgc aaataattca
     3421 atcataccat taacacatga ataaatattt aatttaaaaa ataagcatat gaaaagattc
     3481 actattttta ttaaaaaaat atttgtcctg ctggcgtttc tggcgctgac agcaaatagt
     3541 tcggtgtggg gacaagcctg tgattatttc acggctgcca attccaccac tgttacgggt
     3601 tggaccgaac aagtcggaga ttggcaaatt tacaacaata ccctacaatc gcctgccacc
     3661 gctgagtgga attatattac ccgcgacggc agcacgcaaa caaacggttg tgttagcctg
     3721 cgagccacat ataccggagc cgccggaact aagtatatag gcgcggttgg aagatattct
     3781 gatgccagta actacctgat ggccaaaata caagataatg gttcggcagg ttattgggac
     3841 tcctatttca tttacgaaaa tggaagtatt atcgccaatg cttcaggatt aaatttcggc
     3901 accgatgtga atattgcact ggaatataac ggcactgcca tttcgatgaa gatagatacc
     3961 gacctgaatg gaacctggga ttatacttac acagccacat ccacaaccac caactcaggc
     4021 ttatgcggcg tgggtgctta caatctggca aatgtagata actggtgcta tggctctact
     4081 tgcatcacat gcgctccgcc tgccaatgaa acatgcgcgg gagcaacagc catttattat
     4141 tataatgacc cctaccatac atccggagtt ttgggatgca ctgacgattg cagcggtacc
     4201 gccaattttg atgtctttta taccttcacg ccacaatata ccggttctta cacagccgat
     4261 atgggattaa gtgccggaga cacctatatg agaatttttt ccggctcctg ttgcggaacc
     4321 cttgtagctt ctgacgacga cagctatggc gatatggacc caagcatcac attgaccttg
     4381 acagccggta caacttattg gtttgaatgc ggcagctggt caagcagcgg tatgcaaaac
     4441 tcaccctaca atttcaacct ggtacgcaac tgcacgccgc cagcaaacga aacctgttcc
     4501 acgccaacac ctatctcgta ttcacaaatt gcggcagggt tcaacacttc cggcacactg
     4561 ggctgtgccg acgactgcgc cggttacggc aatgatgttt ttttcagcta cgattgcacc
     4621 tgtacaggca gttacacttt tgacatgaga gacagcgacg gagacaccta catgaggatt
     4681 tattcaggat cctgctgtgg caccctggta gcttcggatg atgacagtta tggcgacatg
     4741 gacccgacca ttacagtcac ccttacagcc ggaaccacct attggattga atgtgccagt
     4801 tatacaacta gcagttatat gtctggcaca tactataatt tatatgcaag taccacatgc
     4861 atattgactc cgggatgcgg aaacacctat ctgggaaaca tcacaccttc gtcatgttat
     4921 ccgcaggatg ccaacttcgc tgccagcaca attccttatt ggtcatttaa tgctactgca
     4981 ggaacaacgt ataatttctc tctgggcgcc agcgcagaag acacatacct gcatctgtat
     5041 aatgatgtaa tgacggaagt agccaccaac gacgatagcg ggcctttcta ttctggatcc
     5101 ccagcctctc tgagctatac ctgcactact agcggcacct attatattac tgcaagccac
     5161 tggacatgca acacgattac aacagccgga tatctcacct actggagttc cggagcgccc
     5221 tacgaaaata catcacttca gactattact cccagtatcg cctggcaaaa tcaaccctat
     5281 gctgctggca gcatggatgt ctacagattc tatggtacag ctggattaat ctatgatttc
     5341 agcctgtgca gcaatacgga agattcatat attcaaatat ataattccac atatgacctt
     5401 ttggccgata atgatgacag cgggccctgg tgtacaggag ttgctgcttc tataagcttt
     5461 accgcacccg caaccgacta ttatattgtt cagatcagac atctgggctg ttcaggcttt
     5521 accaatgccg gcgatctggc ataccgtgtg cagcttgcat gcactgttgg ctgtggcgat
     5581 acc
//
LOCUS       DAOSFP010000134         5572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12908_length_5572_cov_4.660504, whole genome shotgun sequence.
ACCESSION   DAOSFP010000134 DAOSFP010000000
VERSION     DAOSFP010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12908_length_5572_cov_4.660504"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..487)
                     /gene="raiA"
                     /locus_tag="PLI16_05110"
     CDS             complement(188..487)
                     /gene="raiA"
                     /locus_tag="PLI16_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOH83972.1"
                     /translation="MKVNITSLHFKTDKKLESYIQEKVDKLSTLYDGLLSSEVTLRLE
                     KNESEKNKIAEIHLSMRGNDLFAKKQSSTFEQATDTAVEALRKQLVKHKEKIKGI"
     gene            complement(505..1389)
                     /locus_tag="PLI16_05115"
     CDS             complement(505..1389)
                     /locus_tag="PLI16_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOH83973.1"
                     /translation="MLEGFLHYLQYEKRFSPYTVISYKTDLEQFSEYLALQYGITEIA
                     DASYQMIRSWFVSLMEQHITPRSVNRKKAALNSFFKYLNKHGLLTENPMNKVLSPKSV
                     KRLPAFVEEEKMKTLLDEYIPQQDNSFITLRDQLIIEMLYNTGMRLSELVNLKTADVN
                     GFSMTIKVLGKRNKERIIPYNNRLQKLIKEYIDLRQKTILEPCDYFFVTTKGKKVYQK
                     LVYRVVIYYLNTITSQDKKSPHVLRHTFATHMLNHGADLNSIKELLGHANLSATQVYT
                     HNTIEKLKTVYKQSHPKA"
     gene            complement(1501..1695)
                     /gene="rpsU"
                     /locus_tag="PLI16_05120"
     CDS             complement(1501..1695)
                     /gene="rpsU"
                     /locus_tag="PLI16_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOH83974.1"
                     /translation="MIIVPVKEGENIDKALKKYKRKFEKTGVVKELRERQKFTKPSVK
                     RREEVMKAAYIQQLNDERNK"
     gene            1869..2438
                     /locus_tag="PLI16_05125"
     CDS             1869..2438
                     /locus_tag="PLI16_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83975.1"
                     /translation="MHTIEPYYNWRHLYIASEDVRSPFYGNTYSEFEFSYAVYNYLIH
                     PQWDCFGSATLYIKLLFSDYRKKFAILEFIGEWNDCLYNDIKFLKENVIDILTGNGIN
                     KFILIGENILNFHYSDDSYYQEWFDDIEDGWIACVNFREHVVKEFEQGHIDYYLAFAE
                     KLNQMLWRRSDPQQFFELVSEMMTKRLNP"
     gene            2441..2713
                     /locus_tag="PLI16_05130"
     CDS             2441..2713
                     /locus_tag="PLI16_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOH83976.1"
                     /translation="MKIHVNISVSGRVQKIGFRYSTLEVANQLGIAGFVQNQPDGSVY
                     IEAEGPEESVETFITWCHQGPPWARVDDVRVEKTALRNFDSFEIIG"
     gene            complement(2723..3391)
                     /gene="mnmD"
                     /locus_tag="PLI16_05135"
     CDS             complement(2723..3391)
                     /gene="mnmD"
                     /locus_tag="PLI16_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOH83977.1"
                     /translation="MESKIIMTADGSQTIFIPELNECYHSRNGAITESAHIFINAGFR
                     LAAQKNEALNILEVGFGTGLNALLTLIEANKTKRDVFYVGVEPDRVDKKMLTALNYPS
                     LMAIPNSSDYFYKIHNTTSQRQLSDYFTLSIHEEKIQEITTENSFFHLVYFDAFAPAI
                     CPELWNEAVFQKLWNSLTNDGILVTYSCKGEVKRALKAVGFSIEKLPGPPGKREFIRA
                     TKKR"
     gene            complement(3394..4686)
                     /locus_tag="PLI16_05140"
     CDS             complement(3394..4686)
                     /locus_tag="PLI16_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83978.1"
                     /translation="MYKVWLLLLTALLFFCSCAKNNDTSAPQINITSPLENQLFQVGD
                     TVQLIAGICDDSKLNHVSFRIVNSDFVSATPAITIYPENNCTEINHGIAISDMMLESG
                     TYYVMVTAFDGTNTTNGYRKIGINAVEKRLSFILVLTKNDDQVTVHKIDSLNAVSEVK
                     TIFSDYCGSAVSSDARQFYIGGRFTSGVSVFSLDNWQPQWQVPVVVNPPFPYFEAIDV
                     HNMQLFVSFREGRFAIYDHAGNIIASRLVENGEFPKAFLPFKDYLITFETSPDGMQKH
                     LLAYFVPSMAVYKKIFVNYDILKIFPCNAEECLLFCNSGNLGVVKLFSLSTGAVTDKY
                     VFPEGAFDGVACIDGSQYLLISGNEIWYFSYSQKGFTLWATAVNPRCLMYEQTEGCFY
                     FAENQHQVRKNRLSPQENLGTYTFQDTIINILPVYNRD"
     gene            4819..5211
                     /locus_tag="PLI16_05145"
     CDS             4819..5211
                     /locus_tag="PLI16_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83979.1"
                     /translation="METYICPFCKGHLNVNDNIVLLVRNRHNQQGIVFLNTELGNYES
                     QIISSIKFEKGEVCEFFCPYCHTNIEYHKEKTNLVKLLHEDEKGKITQVIFSKIYGEE
                     ATYHIDESKVMSYGEHAKKYMDPEWFLR"
     gene            complement(5259..>5572)
                     /locus_tag="PLI16_05150"
     CDS             complement(5259..>5572)
                     /locus_tag="PLI16_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004373246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOH83980.1"
                     /translation="ADLAMDAAACIGCGACVAACANASAMLFVGAKISQFALLPQGKT
                     EASERVRKMVREMDRLGFGNCSNTYACEAECPKEIKVTNIARLNRQFIKAKAGAEKYE
                     L"
BASE COUNT         1661 a         1080 c         1110 g         1721 t
ORIGIN      
        1 gattcgaacc cccgacccgc tgattacaaa tcagctgctc tggccaactg agctattccg
       61 gcgtagccac tgcttactta atattgacaa tcagatgttt atataaaaaa agcagtcctc
      121 tcataaaaaa ggactgcaaa tgtacacaga aataagttat caacaaagaa aaatattatt
      181 ttttttttca gatgccttta attttttctt tatgtttgac taattgcttg cgcaaggctt
      241 caacagcagt atcggtggct tgttcaaaag tgctggactg ctttttggca aataaatcat
      301 tgccgcgcat cgacaaatgg atttcggcta tcttgttttt ttcactttca ttcttttcaa
      361 gtcggagcgt aacttctgac gaaagcaggc cgtcataaag agttgataat ttgtcgactt
      421 tttcctggat ataactctca agttttttat ccgttttgaa gtgcaatgat gtaatgttga
      481 ctttcataaa aaacctcctt ttttttaagc ttttgggtga gattgtttgt aaactgtttt
      541 taatttttca atggtattat gcgtatatac ctgagttgcc gaaaggttgg catgaccaag
      601 cagttccttg atggaattaa gatcggctcc gtggttgagc atgtgggtgg caaaagtatg
      661 tcgcaacacg tgtggacttt ttttatcctg cgaagttatg gtgttaagat aataaattac
      721 gacacgatat acaagttttt gataaacttt ttttcctttt gtggttacaa aaaaataatc
      781 gcatggttcc aaaatggttt tttgccgcaa atcgatgtat tctttaatga gtttttgcaa
      841 cctattatta taaggtatga tacgttcttt gtttcttttg ccaagcactt ttattgtcat
      901 ggaaaaacca ttaacatcag cggtcttcag gttaaccagt tcggaaaggc gcattcctgt
      961 attatacagc atttcgatga tgagttggtc gcgcagggta ataaaactat tatcctgttg
     1021 gggaatatac tcatcaagca atgttttcat tttttcttct tcaacaaaag ccggcagtcg
     1081 tttaacggat ttgggcgaca gcactttgtt catggggttt tcggttaaca atccatgctt
     1141 attgagatat ttaaagaatg aattgagtgc tgcttttttc cggtttacgg aacgcggtgt
     1201 gatatgttgt tccattagag aaacaaacca tgagcgtatc atttgatagg aagcatctgc
     1261 aatttcagtg attccatact gcagtgcaag gtattctgaa aattgttcca aatcggtttt
     1321 ataactgata accgtataag gagaaaatct tttttcgtat tgtaaataat gtaaaaaacc
     1381 ttccaacatc acataaaaaa aagaataatt ctgtcttctc tataaagtgt gaacgtaaaa
     1441 ttatacattc tattataaaa aagcaagaat tattcttcta aaaaaaggcc ggataacttg
     1501 ctacttattt ctttcgtcgt taagttgctg aatataagca gctttcatga cttcttccct
     1561 acgttttaca gaaggttttg taaatttttg cctttcacgt aattctttaa ctacgccggt
     1621 cttttcaaat ttccttttgt atttcttcag ggctttgtcg atgttttcgc cttcttttac
     1681 aggtacaatg atcataaaaa atctctctct ttcttttgat gattattaaa taatttatcc
     1741 cggttttggg agtgcaaaaa tagtaaatat tttattacaa aaggtttttt ttcatttttt
     1801 tacatgtttc tgaggtttaa aaattgaact ttactgatat ctcttttaga aatcatacct
     1861 ttgcaacaat gcacaccatc gaaccatatt acaactggag acacctctac atcgcttccg
     1921 aagatgtacg ctcgccattt tatggcaata cgtacagtga gtttgaattt tcatacgctg
     1981 tgtataatta cctgatacat ccgcaatggg attgttttgg ctcggcaacg ctatacatta
     2041 aactattatt ttcggactac cggaaaaaat ttgcaatcct tgaatttata ggcgaatgga
     2101 acgactgcct ttacaatgat ataaaattct taaaagaaaa cgtcattgat attttgacag
     2161 gcaacggcat caataagttt attctgatcg gtgaaaacat cctgaacttc cattactctg
     2221 acgattccta ctaccaggag tggttcgatg atattgaaga cgggtggata gcctgtgtaa
     2281 atttcaggga acatgtggta aaggaatttg aacaagggca tatagattat tacctggcct
     2341 ttgcagaaaa actcaatcag atgctctggc gcaggtcgga tccacagcag tttttcgaat
     2401 tggtttcgga aatgatgact aaacggctta acccataata atgaaaattc acgtaaacat
     2461 atccgtatcg ggacgtgtgc aaaaaatcgg atttcggtac agtacgctgg aagtggcaaa
     2521 ccagttgggt atagcaggat ttgtacaaaa ccagcccgac ggcagtgttt atatcgaagc
     2581 cgaaggacct gaagaatcgg ttgaaacatt catcacctgg tgtcatcagg ggccgccatg
     2641 ggcgcgtgtg gatgatgtaa gggttgaaaa aacagccttg cgcaacttcg acagttttga
     2701 aataattggc tgaaaagaag ttttaccttt tttttgtagc cctaataaac tctctctttc
     2761 cgggagggcc gggaagtttt tcgatactga acccgacagc ttttaaggct cttttaactt
     2821 cgcctttgca ggaatatgtc accagaattc catcgttcgt caaagaattc caaagttttt
     2881 gaaacaccgc ttcattccat agttccggac atattgcagg agcaaaggca tcgaaataaa
     2941 ccaggtgaaa aaatgaattt tccgtcgtga tttcctgtat tttttcttcg tgtatgctta
     3001 atgtaaaata atcagaaagt tgacgctgag atgtggtgtt gtgaatttta taaaaataat
     3061 cagagctatt gggaatcgcc atcagcgagg gataattgag tgccgtcaac atttttttat
     3121 ccaccctgtc aggttccaca cctacataaa aaacatcccg ctttgtttta ttagcttcaa
     3181 tcaatgtcag caacgcattc aggccggtac caaagcccac ttccagaata ttcagcgctt
     3241 cgtttttttg agccgccagc cgaaagccgg cattaataaa aatatgtgcg gattcggtta
     3301 tggctccgtt cctggaatga taacattcgt ttagttcagg gataaaaatg gtttgggaac
     3361 catcagcagt cattattatt ttgctttcca taatcaatcc ctgttataca ccggcagtat
     3421 gttaataatc gtatcctgaa aggtataggt gcccaggttt tcttgtggag ataaacggtt
     3481 ctttcgaacc tggtgctggt tttctgcaaa atagaaacag ccttcggttt gctcgtacat
     3541 aaggcagcgg ggattcaccg cagtggccca aagcgtaaaa cctttttggc tatatgaaaa
     3601 ataccagatt tcatttcctg atatcaacaa gtattgggag ccgtcaatac aggccacgcc
     3661 gtcgaaggct ccttcgggaa acacatattt atcggtaaca gcgcccgtgg aaagcgagaa
     3721 aagctttacc acgcccagat ttccgctatt gcagaacagc aggcattctt cggcattgca
     3781 cggaaatatt ttcagaatat cataattcac gaatattttt ttataaacag ccatcgatgg
     3841 aacaaaatag gccaacagat gtttctgcat accgtcagga ctggtttcaa aagtaatcag
     3901 gtagtctttg aaaggcaaaa aagccttcgg aaattctccg ttttcaacca ggcgtgacgc
     3961 gatgatattg ccggcatgat cataaatggc aaaccttcct tcgcggaaag agacaaaaag
     4021 ttgcatatta tgcacgtcta tagcttcaaa ataagggaaa ggcggattaa ccaccaccgg
     4081 aacctgccac tgtggctgcc aattgtcaag gctgaagaca gaaacacctg atgtaaatct
     4141 tcctccgata taaaattgcc gggcatcact gcttacagcc gagccgcagt aatcagaaaa
     4201 gatggttttt acctccgaaa cagcattcag actgtcaatt ttgtgcacag taacctggtc
     4261 gtcatttttt gttagtacga gaatgaatga taatcttttt tcgaccgcgt tgatgcctat
     4321 tttacgatac ccgtttgtag tgttggtgcc gtcgaaagct gttaccatca cataatacgt
     4381 gccggattca agcatcatat cactgatggc gataccgtgg tttatctccg tacaattatt
     4441 ttcaggataa atagttatag caggagtagc tgaaacaaaa tccgaattga ctatcctgaa
     4501 actcacatgg ttgagcttgc tatcgtcaca tatcccggca attagctgca ctgtatcacc
     4561 cacctggaaa agctgatttt caagcggact ggtaatattt atctgagggg ctgatgtatc
     4621 gttgtttttg gcacaagagc aaaaaaacaa caaggccgtc aataatagta accatacttt
     4681 atacatagta acaaaaatac aaaaacagta tcaaaaaaat ggaatatctc aagtttatac
     4741 ttgcaaaagc cataaaaatt cagtaccttt atataattaa ttttttaaaa acaatattca
     4801 tttaaaatat tggggattat ggaaacttat atttgtccct tttgcaaagg ccatctgaat
     4861 gtaaacgaca atattgtttt gctagtgcgc aacaggcaca atcagcaggg aatcgtgttt
     4921 ctaaataccg aactgggcaa ttatgaaagc cagattattt cgtcgattaa atttgaaaaa
     4981 ggcgaggtgt gtgaattttt ttgcccttat tgtcatacca acatcgaata ccacaaagaa
     5041 aaaacaaacc ttgtgaaact gctacatgaa gatgaaaagg gcaaaataac ccaggttatc
     5101 ttttcaaaga tttatggaga ggaagctacc tatcacatcg acgaaagtaa ggtgatgtcg
     5161 tatggagagc atgccaaaaa atatatggat cccgagtggt tcctgagata gcaaaaggca
     5221 taaacatacc tggcgtgtga tatgctgaaa aatcaggctt atagttcata tttttcggcg
     5281 cctgctttag ctttaataaa ctgcctgttg agccttgcta tattggtaac ctttatttcc
     5341 ttgggacatt cggcttcgca ggcataggta ttgctgcagt ttccaaaacc cagccggtcc
     5401 atttcgcgca ccattttgcg gacccgttca gatgcttctg ttttcccctg gggtaagagt
     5461 gcaaactgcg aaattttggc gcccacaaaa agcattgcag aggcattggc acaggcagcc
     5521 acgcaggcgc cacatccgat acaggcggca gcatccatgg ccagatcggc at
//
LOCUS       DAOSFP010000135         5547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_12989_length_5547_cov_3.927713, whole genome shotgun sequence.
ACCESSION   DAOSFP010000135 DAOSFP010000000
VERSION     DAOSFP010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_5547_cov_3.927713"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..714
                     /locus_tag="PLI16_05155"
     CDS             94..714
                     /locus_tag="PLI16_05155"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOH83981.1"
                     /translation="MLLKIHPQNPSDRQIKMAVECLAGGGIVVYPTDTVYGVACDIYK
                     PKALERIARLKNINLEKANFSFICYDLSTLSEYTRPISNTVYKLMRKSLPGPFTYILE
                     ANNNVPKIFHSKKRTIGIRVPDNNIARELVRHLGNPLASTSIHDEDEIIEYTTDPELI
                     HEKFHKLVDMVIDGGYGDNEASTVIDCTSGDPVLVRQGKGIIEELL"
     gene            717..1049
                     /locus_tag="PLI16_05160"
     CDS             717..1049
                     /locus_tag="PLI16_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH83982.1"
                     /translation="MDFVEGNIFTGLASQSKEEIFNPLFSSKNIKIEQIISSGQTSPA
                     RGWYDQEQHEWVVLVEGEATLEFENSQKKKLQKGDYIFIPAHTKHKVFYTSANPPCIW
                     LAVFFNDN"
     gene            1099..1434
                     /locus_tag="PLI16_05165"
     CDS             1099..1434
                     /locus_tag="PLI16_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HOH83983.1"
                     /translation="MSEDNFIIPPEKIKDLIEPMGFCLVSNKITVEGEKVGFMYREKP
                     EDKNDSGWRFLSGTETQDYVDNPHTSKIMDTNTVANYDQAIIPYLNLPYGTELERLQD
                     SDEFVVMEN"
     gene            complement(1510..1995)
                     /locus_tag="PLI16_05170"
     CDS             complement(1510..1995)
                     /locus_tag="PLI16_05170"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOH83984.1"
                     /translation="MKKIINPYTDYPGYNCFGCAPGNTTGLKMEFFEEGDYICSNWMP
                     GESFQGHRGVLHGGIQQVMMDEIGSWVVHLKLHTSGVTARMQTRFLKPVYIMAGQIHL
                     KASLVSMKKNFAEIDVALYDGNGSLCAKSTVVYYTFDEKEAREKYHYPGIEAFYDTHE
                     V"
     gene            2150..2533
                     /locus_tag="PLI16_05175"
     CDS             2150..2533
                     /locus_tag="PLI16_05175"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HOH83985.1"
                     /translation="MEWLLGQWDIRTMNYYYSENWRKENDTSFTGKSIMIVSGDTVLN
                     DEMNIGSNKQVIFLSSKSKINYDSKKNLFKLTKVKKDRIIFENPANPEEKKVTYILST
                     PTMLNIHIEGNENTVESYGLHKINK"
     gene            2608..3300
                     /locus_tag="PLI16_05180"
     CDS             2608..3300
                     /locus_tag="PLI16_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83986.1"
                     /translation="MAKKVDKTEDKILAVENALGKGEQFLEKNKNLLFYVLLGIIVVI
                     GGVFAYNKFVRIPKEREAQSVMFYAEQYFEQDSLDLAINGDGTNPGFLQIVDDYGSTK
                     QGNLARYYLGICYLKTGEYQAAIDNLEDFDSDDHIVAPMALGAIGDAYMELGETEKAI
                     NYYQKAAEKDNNDFTAPMFLIKAGWAYESLNQWDKAIEVYTKIKKEHFKSFEARDIEK
                     YIAYAKGMQETK"
     gene            3307..3792
                     /gene="ribH"
                     /locus_tag="PLI16_05185"
     CDS             3307..3792
                     /gene="ribH"
                     /locus_tag="PLI16_05185"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOH83987.1"
                     /translation="MSSKKKNLSEYTEVSGLLSPDSTIGIVVSEWNPEITESLSKAAV
                     ETLLKHGIKSANIIIEHVPGSFELPLGAQYLIKNKNVDAVICLGCVIQGETRHFEFIC
                     QAVSQHILRLNIDHNLPVIFGVLTTDNFQQAKDRAGGKHGNKGTEAAVAAIKMLLLRD
                     K"
     gene            3824..4789
                     /gene="fmt"
                     /locus_tag="PLI16_05190"
     CDS             3824..4789
                     /gene="fmt"
                     /locus_tag="PLI16_05190"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOH83988.1"
                     /translation="MKDVRIVFMGTPEIAVESLKRLLAKGKNIVSVVTTPDKPAGRGL
                     QLKESEVKKFAVANKLPLLQPENFTEESFVKNISDLSPDIIIVVAFRKISDDIIKIPT
                     LGAFNLHASLLPQYRGAAPINWAIINGETETGLTTFFLNNKIDTGNIILQRKVPILPE
                     YNASALYDVMKSTGAELVIETVNTIIHGDYQTFEQNNLEIPANNLKKAPKIFKEHCKI
                     DWHNKTVAIHNLIRGLSYIPGAYSELKSPDGKIFLLKILQTEYAVNTGIQEPGTIETD
                     NSRYLRIYTSDGYLNVKELQLSGKKIMKIEEFLRGFKMNNRWMTA"
     gene            4972..5244
                     /locus_tag="PLI16_05195"
     CDS             4972..5244
                     /locus_tag="PLI16_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOH83989.1"
                     /translation="MNKAELIDAMAGEAKLTKADAKKALDAFIKATSVALKKGDRVAL
                     VGFGSFGVAKRAARKGRNPQTGKEIKIAAKKVVKFKAGAELAAKVK"
     gene            5298..>5547
                     /locus_tag="PLI16_05200"
     CDS             5298..>5547
                     /locus_tag="PLI16_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83990.1"
                     /translation="MKNEKYLIALIEYLSEFVTEKRLEKFNEIISSRTRHITIGLEDI
                     YQPHNASAVLRSCECLGIQDVHIIENNNKYEVNPDVALG"
BASE COUNT         1922 a         1130 c         1034 g         1461 t
ORIGIN      
        1 ctcccgcaaa ctcccccagg tccggaaggc agcaagggta ggcggttgta gcggtgcgat
       61 ataagtagct tacccttttt cgttgaaaac tatatgttat taaaaataca tccacaaaac
      121 ccttccgacc ggcaaataaa aatggccgtt gaatgtcttg ccggcggagg tattgttgtt
      181 tatcccaccg acaccgtgta tggcgttgcc tgcgatattt acaagccaaa agcccttgaa
      241 cgaatcgccc gcctgaaaaa catcaacctg gaaaaggcca acttttcttt tatctgttac
      301 gacctcagca ccctttctga atataccaga cccatatcca acaccgtgta caaacttatg
      361 cggaaatcac tgcccgggcc gtttacctat atccttgaag ccaataataa tgtgccaaaa
      421 atttttcaca gcaaaaaaag gactatcggt atccgtgtgc ccgataacaa tattgcccgt
      481 gaacttgtcc gtcatctggg caacccgtta gcatcaacgt ccatacatga tgaagacgaa
      541 atcatagagt atactaccga tccggagctt attcacgaaa aattccacaa actggtggat
      601 atggtaatcg acggaggcta tggtgacaat gaggcctcta cagtgataga ctgcacatcg
      661 ggagaccccg tcttggtgcg acagggaaaa ggtatcatcg aagaattact ataaccatgg
      721 actttgttga aggaaatatc ttcacagggc ttgccagcca aagtaaagaa gaaattttca
      781 acccgctttt ttccagcaaa aacatcaaaa tagaacaaat catttcttcg gggcaaacct
      841 cgccagcccg cgggtggtac gaccaggaac aacacgaatg ggttgttttg gttgaaggcg
      901 aagctacact ggaatttgaa aacagtcaga aaaaaaaatt gcaaaaaggt gattatatat
      961 ttattccggc acacacaaaa cacaaagtat tttatacctc agcaaatccc ccatgcattt
     1021 ggcttgccgt attttttaat gacaattaga attagtaatt aagggaatct ttcaaatcaa
     1081 atacattata ttatatctat gagtgaagat aatttcataa tccctcccga aaaaatcaaa
     1141 gacctcattg agcctatggg tttttgtctg gtttcaaata aaatcactgt agagggagaa
     1201 aaagtaggtt tcatgtaccg tgaaaaacct gaagataaga acgacagcgg ctggcggttt
     1261 ctatcgggca ctgaaacaca ggattatgta gacaacccgc ataccagcaa aatcatggat
     1321 acaaatacgg tggccaatta cgaccaggcc atcatcccgt acctgaacct gccttacgga
     1381 actgaattgg aaaggctaca ggatagtgat gaatttgttg tcatggaaaa ctgattcttt
     1441 tccctttaca agcgtttaca caatcacctt ggccgggcca atccggatac catcaccact
     1501 acatattact cacacctcat gtgtatcata aaatgcctca ataccgggat agtggtactt
     1561 ttctctggcc tctttttcat caaaagtata atatactacc gttgattttg cgcacaacga
     1621 accattccca tcatagagcg ccacatcaat ttcagcaaaa ttttttttca tagaaaccaa
     1681 actggctttc agatgaatct gacctgccat gatataaaca ggttttagaa aacgggtttg
     1741 catacgtgcc gtaactcccg aagtatggag ctttaggtgc accacccaac ttccaatctc
     1801 atccatcatc acttgttgaa tacctccatg cagtacaccc ctatgtccct gaaaagactc
     1861 gccgggcatc cagtttgaac aaatatagtc accttcttca aaaaattcca ttttcagacc
     1921 tgttgtattg ccgggagcgc aaccaaaaca attatagcca ggataatcgg tgtacggatt
     1981 aataatcttt ttcatataag attttttaca aaaatatcaa ttacttttat ggcaaaaatc
     2041 gaaaacatca atgagacgaa taattatcta tgtttttttt cttattatcc tcttttcatg
     2101 taacacagcc aatcattttg tatctcataa aataagtacc tttaaaaaaa tggaatggtt
     2161 actgggccaa tgggacatta gaaccatgaa ctattattac agtgaaaact ggcgcaaaga
     2221 aaacgacacc tcttttaccg gaaaaagcat catgatagtt agcggagata ccgttctcaa
     2281 cgacgaaatg aatataggct cgaataaaca ggtaattttt ctgtcaagca aaagcaaaat
     2341 aaattacgac agcaaaaaaa atcttttcaa actcacgaaa gtaaaaaaag accgcatcat
     2401 ctttgaaaat cctgccaatc cggaagagaa aaaagtcacc tacatattaa gcacccccac
     2461 aatgctaaac atccatattg aaggcaacga aaatacggtg gaaagttatg gattacataa
     2521 aatcaataaa tgaaacgcaa aaaataatta aatacactca tattctaaaa aaaaatttat
     2581 ctttgcacct ctaaaaacat taaggaaatg gcaaaaaaag ttgacaaaac agaagataaa
     2641 attttagcag tagaaaacgc cctgggaaaa ggagaacaat ttcttgaaaa gaacaaaaac
     2701 ctcttatttt atgttcttct cggaataatc gtggttattg gcggagtctt tgcctacaac
     2761 aaattcgtcc gtattcccaa agaacgtgaa gcacaatcag tgatgtttta tgcagaacaa
     2821 tattttgagc aggactcttt agacctggca ataaatggcg acggaacaaa tcccggattt
     2881 ttacaaattg ttgacgacta cggttcaacc aaacagggca acctggctcg ttattacctg
     2941 ggcatttgct acctgaaaac cggcgaatac caggccgcca tagataatct ggaagatttc
     3001 gattcagacg atcatattgt tgcccctatg gcacttggcg ctattggtga tgcttatatg
     3061 gaacttggcg agacagaaaa agccataaac tattatcaaa aagccgccga aaaagacaac
     3121 aatgatttta ctgctcccat gtttttaata aaagccggtt gggcatacga aagtcttaac
     3181 cagtgggaca aagccattga ggtatatacc aaaattaaaa aagaacactt caaaagtttt
     3241 gaagcacgcg atattgaaaa atatattgct tatgccaaag gcatgcagga aaccaaataa
     3301 cacctgatga gttcaaagaa aaaaaacctc tctgaatata ctgaagtttc cggattatta
     3361 tctcccgata gcaccatagg gatagttgtt tcggagtgga acccagaaat cacagaatcc
     3421 ttaagtaaag ccgccgtaga aacacttttg aaacacggca taaaatcagc gaatatcatc
     3481 attgagcatg tgcccgggag ttttgaactt cctttaggcg ctcagtattt aattaaaaac
     3541 aaaaatgtag atgctgtgat atgccttgga tgcgttatac aaggagaaac acgtcatttt
     3601 gaatttatct gtcaggcagt aagtcagcac atcttacgcc ttaatatcga tcacaacctg
     3661 cctgttattt tcggggtatt aaccaccgat aatttccagc aggcaaaaga ccgtgcaggt
     3721 ggcaaacatg gcaacaaagg caccgaggca gccgtggccg cgattaaaat gttgcttcta
     3781 cgcgacaaat aatttttcaa atcaatataa acgggagtag tttatgaaag acgtacgcat
     3841 tgtatttatg ggtacacctg aaatcgccgt agaatcgcta aaacggttgc tggcaaaagg
     3901 gaaaaacata gtttccgttg ttacaacccc agacaaaccg gcaggaagag gccttcagtt
     3961 aaaagaatct gaagtaaaaa aatttgctgt ggcaaataag cttccactac tccagccaga
     4021 aaactttact gaggaaagtt ttgtaaaaaa tattagtgat ttatctcctg acattattat
     4081 tgttgtagct ttccgtaaaa tatctgatga tattattaaa atacccaccc tgggagcatt
     4141 taatcttcac gcatcattgt taccccaata cagaggtgca gcacccatca actgggcaat
     4201 aattaatggt gaaacagaaa cgggacttac cacatttttt ctgaataata aaattgacac
     4261 aggcaatatc atattgcaaa gaaaagtccc tattttgccc gaatacaatg cttccgcctt
     4321 atatgatgtc atgaaatcaa caggtgctga actggtcatt gaaactgtta atacaattat
     4381 acatggtgat tatcagactt ttgagcagaa taatttggaa ataccagcta ataatttaaa
     4441 aaaagctcca aaaatattta aagaacattg taaaattgac tggcataaca aaaccgttgc
     4501 cattcataat cttataaggg gattaagcta tattccagga gcatacagcg aattgaagtc
     4561 ccctgatggg aaaatattcc tccttaaaat acttcaaact gaatacgccg tcaatacagg
     4621 gatacaggag ccaggcacca ttgaaaccga caacagcagg tacttacgga tatatacatc
     4681 agatgggtac ctaaatgtta aagagctgca gctatcgggg aaaaaaatta tgaagattga
     4741 ggagtttctc aggggtttca aaatgaacaa tcgctggatg actgcttgat ttgagccgat
     4801 aaatcaccgg gaaagcctgt gaacagcgga atgttttcaa caaaaacccc attttattaa
     4861 caaaatcaag ctttttagcg cataatactt gttttgttga ttttattttg tatttttgtt
     4921 tcattattaa cttaattaaa ttaattaaca tttaattaaa atcttttaaa catgaacaaa
     4981 gcagaattaa ttgatgcaat ggctggagaa gccaaattaa caaaagctga tgcaaaaaaa
     5041 gcattggacg ctttcattaa agcaacctca gtagccctga aaaaaggtga cagagtagct
     5101 ttagtaggtt tcggatcttt cggcgtagca aaaagagctg caagaaaagg ccgcaatcct
     5161 caaactggca aagaaattaa aatcgctgcc aagaaagtgg taaaattcaa agctggtgct
     5221 gaattggctg caaaagttaa gtaacattgc ttcaagctgt taaaagtcct gaattttaat
     5281 tcaggacttt tttttatatg aaaaacgaga aatacctaat agcgctcata gagtatctca
     5341 gcgaatttgt cacagaaaaa cgtctggaaa aattcaatga aataatctct tccagaaccc
     5401 ggcacatcac cattggcctg gaagacatat atcaaccgca taatgccagt gcggttttgc
     5461 gctcatgcga atgcctggga attcaagatg tacacatcat tgaaaacaat aataaatacg
     5521 aagtcaaccc cgatgtggct ctgggct
//
LOCUS       DAOSFP010000136         5502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13108_length_5502_cov_4.264549, whole genome shotgun sequence.
ACCESSION   DAOSFP010000136 DAOSFP010000000
VERSION     DAOSFP010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5502
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13108_length_5502_cov_4.264549"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(355..1926)
                     /locus_tag="PLI16_05205"
     CDS             complement(355..1926)
                     /locus_tag="PLI16_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83991.1"
                     /translation="MKKILVFLSLVLSVVFLFAQNAIKPVQINVFKNGTYFIAKEGNV
                     PVKKGYAMLELPKQPLLGTYWLNAAKENKITSVCFVTDTIKKTKKPQNFNDIFKGSVG
                     KKVRFSYAVGVDKNLREISGVSQEYYEGSGIAKIKLADNKTTFYPVASVVEFTIDDSN
                     VGELTMDSLVRVAKIYFDKSISATDLRVVYMQTGIQWIPSYNIRIINDNELQLEMKAL
                     VENYSEDIENAELTLTVGNPQFFYGVQYDPIYNNMLTSLYEVKPMLPVAQSQTYFTSN
                     VFVAEERTTTGTDFNQYADYSTQGEKTNDLYMYRIGKVNLPMQSKSSFQIFSVKVPYK
                     DVYEVNIGDVANYSYYSYISNDPEKRMDVYHSLLLTNNTTYPFTTAPVFVQDEHMQPL
                     AQDRIKYTPTGADISVQLSKAGDVVVKNKEEEVKKEENVKKIGKTYYNKVTVKGMIFI
                     ENLQKKKILLTIKKDLTANALSAGSDGVISKSGRYSGLNPYVNINWELPLNAGEKKSV
                     VYEYEVYAPAGGSSY"
     gene            1985..2731
                     /locus_tag="PLI16_05210"
     CDS             1985..2731
                     /locus_tag="PLI16_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOH83992.1"
                     /translation="MPETNNSASSKRPKGKLYLLPSLLGETSEMSAVLPENTIKITST
                     LTVFIVEELRTARRFLKKIDKNINIDSLEFLVFNEHSIKENISQYIAPMLQGKHAGIL
                     SEAGLPCIADPGSEIVAEAHRQNIDVVPLTGPSSIILALIASGFNGQNFAFHGYLPVD
                     KALRAKKLRQLEMDAVKNNQTQIFIETPYRNLQIIQSISETCHSETMLCVAADLTLPS
                     QKILSKPVSFWKKHHLDIHKRPAVFLLFAP"
     gene            2822..4096
                     /locus_tag="PLI16_05215"
     CDS             2822..4096
                     /locus_tag="PLI16_05215"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH83993.1"
                     /translation="MSILLSNATYIDFQTLEFIPCNILIDNRHSQSIVFTEKEENSTD
                     IKIDCTGKYVTRAFGCGHHHVYSALATGMPAPAKNPSNFLEILQYVWWTLDKCLDQEM
                     IEISALFTAIECAKNGVTFVVDHHSSPNAIKGSLETIAKAFDKVGVSHLLCYEISDRD
                     GEKAREEALEETDTYLANRQGLVGLHASFTVSDYTLKRAKTLTDKFKTGIHIHVAEDR
                     LDEQLCFEQQHRRVVERLNDFGLLSSSKTILSHCLYIDQNERELIKKAGVWIAQNTES
                     NMNNKVGFFNSEGIQQNVMLGTDGMNSDMLQSAKTTFFAGQNFDKTDYADAYRRFRNV
                     NFYFSENGFSGDAENNLVVLDYVPHTDFHRENFLGHFLFQINSKHVQHVISNGRLIVK
                     DRKVVTVNEQDIMLQSRELSKKLWEKMVKLGK"
     gene            4168..>5502
                     /gene="hydA"
                     /locus_tag="PLI16_05220"
     CDS             4168..>5502
                     /gene="hydA"
                     /locus_tag="PLI16_05220"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOH83994.1"
                     /translation="MKVLIKNPFIVNADRSFRSDILIENGSIISIDENIESKDDTIVI
                     DATHLYALPGGIDPHVHMQLPTPAGNSSDDFYSGSLAALTGGTTSIIDFVTPSHGQSQ
                     IEALKLRKSEAAGSLTDYTLHGTVVEFNEDTEEEVRKCIVKEGITSFKTYLAYRGTVG
                     IDYETLRRVMQIVSKYNGTVTVHCEDGDEISRLQQQFLSRGEYTPLYHALSRPEYVES
                     DAVAEVLKLAGETGCKVYLVHVSAKKSIELIAKHKPLRNIFAETCPQYLFLDENNYRK
                     PLPDSLKYVISPPLRKQDSINALWQHIVNGCVDTIGSDHCPFNTIGQKDAGINDFSKI
                     PNGAGGIEYRMQLLFTYGVLQNKISLSRFVELTSTNAARIFGLFPKKGITAVGSDADI
                     VLWNPNTKSTISVQTQFQQCDSNIYEGIALTGAPEYVICRGKIAIVNGKPTAD"
BASE COUNT         1634 a         1205 c         1109 g         1554 t
ORIGIN      
        1 agggtttttc ttggtgtctt ggtgtcttag tggcctttca ttttttttgc cacaagagca
       61 cgaaggcaca aagttgaata aattcctttt tagggtttcc cttggtgtct tagtgtctta
      121 gtgtcttagt ggcctttctt ttttagccac aaaggcacga aggcatgaag ttttataaat
      181 tcttttttag ggtttccctt ggtgtcttag tgtcttagtg gcctttcttt tttagccaca
      241 aaggcacgaa ggcacaaagt tgaataaatt ccttttaggg tttttcttgg tgtcttggtg
      301 tcttagtggc ctttcatttt ttttgccaca aaggtacgaa agcacaaagt ttttttaata
      361 acttgatcca cctgccggtg catacacttc atattcgtaa actactgatt ttttttcacc
      421 agcgtttaaa ggcagttccc agttaatatt tacgtaaggg ttcagcccgc tataccttcc
      481 gcttttggaa ataactccgt cggagccagc cgaaagggca ttggcggtca ggtctttttt
      541 aattgtcagc agtatcttct ttttctgcag attctcaatg aaaatcattc ccttgacggt
      601 aactttattg taataagtct taccgatttt ttttacgttt tcttcttttt tcacttcctc
      661 ttccttgttc tttaccacca catcgcctgc ttttgaaagc tgaacagaaa tatcagcgcc
      721 ggtgggcgta tatttaatcc tgtcctgcgc caggggttgc atatgttcat cctgcacaaa
      781 aacaggagcc gtggtaaagg gataagtggt attgttggta agcaataaag aatgatatac
      841 atccatacgt ttttccgggt cgttgctgat ataactgtaa taagaataat tggccacatc
      901 tccgatattg acctcataaa catccttgta aggtactttt acagaaaaaa tctgaaaact
      961 tgatttgctt tgcatcggca gatttacttt tcctatacgg tacatataca agtcgtttgt
     1021 tttctctccc tgggtcgaat aatcggcata ttgattaaaa tctgtaccgg tagttgttct
     1081 ttcttcagcc acaaaaacat tgctggtaaa ataggtctgg ctttgtgcaa cagggagcat
     1141 gggttttact tcatataggg atgtcagcat attattataa atcgggtcat actgcacgcc
     1201 ataaaaaaac tgcggattgc caacagtgag cgtaagttca gcgttttcaa tatcttcaga
     1261 gtaattttcc accagagctt tcatctctaa ttgtagttca ttgtcattaa taatcctgat
     1321 attataagat ggaatccatt gtatcccggt ttgcatatac accacccgta agtcggtagc
     1381 tgaaatactt ttatcaaaat aaatcttggc cacacgtacc aacgaatcca tggtgagctc
     1441 acccacattg gaatcgtcga tggtaaactc caccaccgat gctacaggat aaaacgtggt
     1501 tttgttatcg gcaagtttta tttttgcaat gcccgaacct tcgtagtatt cctgcgaaac
     1561 cccgctgatt tctctaaggt ttttatcaac tcccacagca tacgaaaaac gaactttttt
     1621 acctaccgag cctttaaaaa tatcattgaa gttttgcggc ttcttggttt tttttattgt
     1681 atcggtaaca aaacacacgc tggtaatttt gttttctttg gctgcattga gccagtaagt
     1741 ccccaacaac ggctgctttg gcagttcgag catggcataa ccttttttta caggcacgtt
     1801 gccttctttg gcaataaaat aagttccgtt tttaaacacg tttatctgta caggcttgat
     1861 ggcgttttgg gcaaaaagaa acaccaccga caaaactaaa ctaagaaaga caaggatttt
     1921 tttcatggct tgaggttttt aaattatttt tgttttcgga aaaggaaaat tttgtccaaa
     1981 tcttatgcca gaaaccaata attcagcgag ttccaaacgc cctaagggca aactttatct
     2041 gttgccttcg ttacttggag aaacatcgga aatgtcagcc gttttgcccg aaaacaccat
     2101 aaaaatcact tcaacactga ctgtattcat agtggaagaa ttgaggacag cccgacgttt
     2161 cttaaaaaag attgacaaaa acatcaatat tgatagttta gagtttttag tattcaacga
     2221 acacagtatc aaggaaaaca taagccaata catcgcgcct atgctgcaag gcaaacacgc
     2281 cggcatactg tctgaggcag gcctgccatg cattgccgac cccggctcgg aaatagtagc
     2341 tgaagcccac aggcaaaaca tcgacgttgt gcccctcaca gggccttcat ccattattct
     2401 tgccctgatt gcttcgggtt tcaacggaca aaacttcgcc tttcacgggt acttgccggt
     2461 agataaagct ctcagggcaa aaaaactccg ccagctggag atggacgccg taaaaaataa
     2521 tcaaacccaa atctttatcg aaacacctta tcgaaacctg caaataatac aatccattag
     2581 tgaaacctgt catagcgaaa ccatgctgtg cgtggctgca gaccttacct tacccagtca
     2641 aaaaatattg tcaaaaccag tgtctttctg gaaaaaacac catctggaca tccataagcg
     2701 accggcagtt tttttgcttt ttgctccctg accgttacaa gaaatttcat tccgatatca
     2761 tgtaaaggaa ccataaaaac tggtagcttt gcatcatcga atattaataa aataaaatat
     2821 catgtcaata ctcttatcca acgccaccta catcgatttt caaacactgg agttcattcc
     2881 ctgcaacatc cttatcgata accgccatag ccaaagcatt gtttttactg agaaagaaga
     2941 aaacagcacc gacataaaaa ttgattgtac cggaaaatat gttaccaggg cttttggctg
     3001 cggacatcat catgtgtatt ccgctcttgc taccggaatg ccggcaccgg ccaaaaatcc
     3061 atccaatttc cttgaaatac tgcaatatgt gtggtggact ctcgacaaat gcctcgacca
     3121 ggagatgatt gaaatcagcg cactgtttac agccatcgaa tgtgccaaaa acggtgttac
     3181 cttcgtggtt gaccaccact cctctcccaa tgccatcaag ggatcgcttg aaactatcgc
     3241 caaggcattc gacaaggtag gcgtttcgca tctcttgtgc tatgagatct ccgaccgcga
     3301 cggagaaaaa gccagagaag aagcactgga agaaaccgat acatacctcg ccaacaggca
     3361 gggattagtg ggccttcatg cgtcgtttac cgtttcggac tatacattaa aacgcgccaa
     3421 aacgctgacc gacaagttta aaactggcat tcatattcat gtggccgaag atcgactcga
     3481 cgaacaattg tgttttgaac aacaacacag acgcgtagtg gaacgactta atgacttcgg
     3541 cttactcagc agcagtaaga ccatactaag ccattgtttg tacatcgacc aaaacgaaag
     3601 agaactaatc aaaaaagcgg gcgtgtggat tgcacaaaac accgaaagta atatgaacaa
     3661 taaagtgggt tttttcaatt cggaaggaat acaacaaaat gttatgctcg gcaccgatgg
     3721 catgaacagc gatatgttgc aaagcgccaa aacaacattt tttgccggac agaattttga
     3781 caaaactgac tatgcagatg cttaccgccg ttttagaaac gtcaactttt acttcagtga
     3841 aaacggattt tccggtgatg cagaaaacaa cctggtggta cttgattatg tgccacatac
     3901 cgattttcac cgtgaaaact ttttaggaca ttttcttttt cagataaatt caaaacatgt
     3961 gcagcatgta atttccaatg gccggctgat tgttaaggac cgcaaagttg tcacggttaa
     4021 tgagcaggac attatgttgc aaagccgtga attaagcaaa aaactgtggg agaaaatggt
     4081 gaaattagga aaatagaatt ttaggttgca ccaaattgca ttattgagaa agtcaaaaaa
     4141 gaaaagttag tcatatcgat gtaaaaaatg aaagttttaa taaaaaaccc gtttattgtc
     4201 aatgcagata gaagttttcg ctccgacatt ctgattgaaa acggaagcat tatcagcatt
     4261 gatgaaaaca tcgaaagcaa ggatgatact attgtgatcg acgccacaca tctgtatgcc
     4321 ttgccgggcg gcatcgatcc tcatgttcac atgcaactgc ccaccccggc aggaaactct
     4381 tcggatgatt tttattcggg cagcctggca gcgctgacag gaggaacaac aagcatcatt
     4441 gattttgtaa caccatcgca cggccagtca caaattgagg ccctgaaact gcgcaaaagc
     4501 gaggccgccg gatcactcac cgattacact ctgcacggca ccgtggtcga gtttaatgaa
     4561 gatacagaag aggaagtcag gaaatgcatc gtaaaagaag gtataacttc ttttaaaaca
     4621 taccttgcat atcgtggcac ggtaggcata gattatgaga cactccgccg ggtgatgcaa
     4681 atagtcagta aatacaacgg gacagttaca gtacattgcg aagatggcga cgaaatcagc
     4741 cgcctgcagc aacaatttct ttcgcgaggt gaatacacgc cgctatatca tgctttgtca
     4801 cggcccgaat acgttgaatc ggatgccgta gccgaagtgt taaaactggc tggtgaaaca
     4861 ggctgcaaag tctatctggt gcacgtttcc gcaaaaaaat ccatcgagct gattgctaaa
     4921 cacaaacctt tgagaaatat ttttgcagaa acctgtcctc aatatttgtt tttggatgaa
     4981 aataattacc ggaaacccct gcccgattca ctgaaatatg taatcagtcc gccactgcgg
     5041 aaacaggaca gcatcaatgc cctgtggcag catattgtaa acggatgcgt ggataccatt
     5101 ggctccgacc attgtccctt caacaccatt ggccaaaaag atgccggcat aaatgatttt
     5161 tcaaagatac ccaacggcgc cggaggcatt gaataccgta tgcaactgct ttttacctac
     5221 ggggttttgc aaaacaaaat atctctcagc cggtttgtag aacttacctc caccaatgcc
     5281 gccagaatat ttggcctttt tccaaaaaaa ggaattactg ctgttggttc agatgccgat
     5341 atcgtgctat ggaatcctaa tacaaaaagc actatttcgg tgcaaactca gtttcagcaa
     5401 tgcgattcga atatctatga aggcatagcg ctgacaggcg ctcccgaata tgttatatgc
     5461 cgtggcaaaa ttgctattgt caatggcaaa cccacggctg at
//
LOCUS       DAOSFP010000137         5462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13224_length_5462_cov_4.314407, whole genome shotgun sequence.
ACCESSION   DAOSFP010000137 DAOSFP010000000
VERSION     DAOSFP010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13224_length_5462_cov_4.314407"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1470
                     /locus_tag="PLI16_05225"
     CDS             <1..1470
                     /locus_tag="PLI16_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH83995.1"
                     /translation="RGLALDKKNNNPVPGALVFLMNDKMVIIDSAIANEKGEYSFPAD
                     YNNNYNLKGQKENYINGFNSVSTYNLGEKKEVTANVLLDKLTLIIQVTDAKTQQRLQK
                     VSVQLKDKKSGMTELFSTDETGEIVKNLTDKKIKDSLCYELQFTKAGYNSKTIDYKHL
                     IIAAGEILIPEKLEKTDPIRIRGVAMDKNTKEILPGTKVILMDNKMTEIAQTLTNEKG
                     AFVFDADYDQEYLLKGQKPSYTDGDNKVTTPGVGKAKDVTADLLLEKVIYVSLKVRVI
                     DAKNNLPVKDATVSLKDMISGKTELFTSDANGELFKPIGIKEINDSLKYELTFSKDEY
                     HTKIADYKYRITKSEEILIVETIGKMEVGTDLAKIIQIKPIYFDLDKYNIRPDAAVEL
                     DKIVKIMNEYPTMVIELGSHTDCRQTYQYNERLSDNRAKSSAAYIRQRITNPERIYGK
                     GYGETQLVNECECEGKKVVPCTEAQHQMNRRTEFIIIKY"
     gene            1620..2633
                     /locus_tag="PLI16_05230"
     CDS             1620..2633
                     /locus_tag="PLI16_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOH83996.1"
                     /translation="MSLIATLQKRIMLLDGAMGTMLQSFRLQEKDFRGTLFTNHPMPL
                     QGCYDILNITNPLIVSRIHHDYLAAGADIISTNTFNAQAVSLSQFGLEKHVREINLQA
                     AKIARAVADGFNSTSTMQPRYVAGIIGPAHETESVPHKTLTPGSHPLAFDALVSVYAE
                     QATALIDGGVDLLIVETIFDLHHVRAALSALAKVFSQKGHILPVMASLTAGEKNGVLL
                     SGHTTDEFLALVLQQEVPLFSVGFNCSPGIGAARPYLEKLSATAPYFVHYYPAAGLPD
                     RRGQYPITPAGMANAMKQYMQQGLVNIVGGCCGTTPEYVAAIAKLAKKYPPRNPQPYQ
                     HQC"
     gene            2757..3887
                     /locus_tag="PLI16_05235"
     CDS             2757..3887
                     /locus_tag="PLI16_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH83997.1"
                     /translation="MKKFFVLQLWLFIAFLKITVVQGQTAVDYYNNGISKYMAKDYAG
                     AVAEYDKAIAIDAGYAMAYNNRGLAKYKLKDYAGAEADYTQAIKFKPEYAMAYLNRGL
                     ARYNLKDFEGELADYNKTIELDSKMASAYYNRALAKESMKDSDGALADYLKAIEYDPK
                     YSKTAYYNIASINYSRKDFTGAIKNFTLAIENNPNDARSYFNRGLAHYELGAYDNAKT
                     DFTKTTELNPQHAEAYYCRGLTKVFMEDIPGAIADYTKTIEINPRHDKAYYSRGVAKQ
                     ELKDHEGAISDFSAALTINPKYAEAYNNRGTAKYNLHDFNGAMEDYSLAIDNNSQYAM
                     AYYNRGLVKIDLKQQESACLDFKNALKYGEAAAKNQLSRHCE"
     gene            4278..>5462
                     /locus_tag="PLI16_05240"
     CDS             4278..>5462
                     /locus_tag="PLI16_05240"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOH83998.1"
                     /translation="MTKRLILLFLLAGNYAMAQLNINTSLTANQLMQNFIGGGVTVFN
                     VVYTGGNASKATFTNGNNTNLGLSEGIILCSGNATNIANPATFFMSNNLNQAGDATLN
                     SINNGTSTFDAAVLEFDFIPVCDSVKFKYVFGSEEYPHSICSQYNDVFAFFITGPNPA
                     GGNFANHNIALIPGTNLLVSVNSVNLGVPGPGLSPSGCLSLGYSNYYVDNLGMGGTTI
                     AFGGFTTPLTARCKVMKDSVYHMKIAVADGQNAIYDSGVFLEANSFSSNVLTVNTCYP
                     DTIFGQNAMEGCSEAIVSFIAPANVTSPTTITYTIGGSATNGVDYNTVGNSVTIPAGH
                     DSAGLVIHPLADENTEGAETVILNITYDCITIIDTITIIDKVSLQVTSGNDAAICSGN
                     SLV"
BASE COUNT         1680 a         1321 c         1139 g         1322 t
ORIGIN      
        1 agagggctgg cactagataa aaaaaacaac aaccctgttc cgggcgccct ggtattcctg
       61 atgaacgata aaatggtaat cattgattcg gcaatagcca acgaaaaagg agaatattca
      121 ttccctgcag attataataa caactacaat ctgaaaggtc aaaaagaaaa ctatatcaac
      181 ggctttaatt ccgtatccac ttataatctg ggcgaaaaga aagaagtaac agccaatgtc
      241 ctgctggaca aactcacgtt aataattcag gtaaccgacg caaagacaca acaaaggctc
      301 caaaaggtca gcgtacaact taaagacaag aaatcaggaa tgacagaact cttcagcacc
      361 gacgaaacag gcgaaatcgt taaaaacctg actgataaaa aaatcaagga cagcctgtgc
      421 tatgagctac agttcaccaa ggcgggatat aattcaaaaa ccattgacta taagcatctt
      481 attatagctg caggagaaat acttatcccg gaaaaactcg aaaaaacaga tccgatccgc
      541 atccgcgggg tggctatgga taaaaatacc aaagaaattt tacccggcac caaagtcatc
      601 ttaatggaca acaaaatgac cgaaattgca cagacgctca ccaacgaaaa aggcgcgttt
      661 gtgtttgatg ccgattacga tcaggagtat ttgctgaaag gacaaaaacc gagctatacc
      721 gatggcgaca ataaagttac cacccccggg gtgggcaaag ccaaggatgt tacagccgat
      781 ctgctgcttg aaaaagtcat atatgtttct ttgaaagtcc gggtgattga cgcaaaaaac
      841 aacctgcccg taaaagacgc taccgtatca cttaaagata tgatctccgg caaaaccgaa
      901 ctgttcactt ccgacgccaa cggggagttg ttcaaaccta ttggcattaa agaaattaac
      961 gacagtctga aatacgaact taccttcagc aaggatgagt accataccaa aattgccgat
     1021 tacaaatatc gcattactaa atcggaagaa atactcattg tagaaaccat tggaaaaatg
     1081 gaagtgggta ccgatttggc aaaaatcatt caaataaaac ccatttattt cgacctcgac
     1141 aagtataata tcaggccgga tgccgctgtg gagctggaca aaattgtcaa aatcatgaat
     1201 gaatacccca ccatggtcat tgagctgggc tcgcataccg actgccgtca gacataccaa
     1261 tacaacgaac gcttgtcgga caaccgggcc aaatcgtcag cagcttacat cagacaacgc
     1321 attaccaacc ctgaaagaat ctatggcaaa ggttatggag aaactcaact ggtaaacgaa
     1381 tgcgaatgcg aaggcaaaaa agttgtacca tgtaccgagg cccagcatca gatgaaccgg
     1441 cgtacagagt ttatcatcat aaagtattaa accggacaca ggcttttaac gccacgccaa
     1501 aaacacatcc tcctcaccgg acacatgcag ctataaccgg gttggcttgt attgctccaa
     1561 aaatgtaaca aaagccctat ctttgcatgt attctctttt atcagaggga aaaaaattta
     1621 tgagcctgat tgcgacactc caaaaaagaa ttatgctcct tgatggcgct atgggcacta
     1681 tgttgcaatc attccggcta caggaaaaag atttcagggg aacgcttttt accaatcatc
     1741 ccatgccact gcaaggctgc tacgacatcc tcaacattac aaatcccttg attgtcagcc
     1801 ggatacacca cgattatctt gccgcggggg cagacattat ttcaacaaac acctttaatg
     1861 cccaggccgt ttctttgtca caattcgggc tggaaaaaca cgtccgggag atcaatctgc
     1921 aagctgcaaa gattgccagg gcggtagccg acgggttcaa tagcaccagt acaatgcaac
     1981 cccgctatgt ggccggtatc atcggccctg cccatgaaac tgagtccgta ccgcataaaa
     2041 ccctcacacc tggctcacac ccccttgctt tcgacgcact tgtcagtgtc tatgccgagc
     2101 aggccacggc actgatagac ggcggcgtgg acctgttgat agtagaaacc atttttgact
     2161 tacatcacgt cagggcggca ttatcggctt tagccaaagt tttttctcaa aaaggccaca
     2221 tcctgcctgt gatggcctcc ctgacagcag gcgaaaaaaa cggggtcctg ctctcaggac
     2281 atactacaga tgagttttta gcccttgtgt tgcaacagga ggtgcctctg ttttccgttg
     2341 ggttcaattg ttcgccggga ataggtgccg cacggcccta tctggaaaaa ctgtcagcaa
     2401 cagcccccta ttttgtgcat tattatccgg ctgccggatt gcctgaccgc cgcggccaat
     2461 accccataac gcccgccggg atggcaaacg caatgaaaca atatatgcaa caaggtctcg
     2521 taaatatcgt gggcggctgt tgtggcacca ccccggaata tgtagcggct atagcaaaat
     2581 tggcaaaaaa atacccgccc cgcaatccac aaccatacca acatcaatgc tgatgctaat
     2641 caccgctcaa tagcccgttt gattttcaaa attccgctca gtaatttaat ggcgtaattt
     2701 ttgagcctgt tcagcgcttt tttacgtaat ttgtaaataa ttttaacatg acggaaatga
     2761 agaaattttt tgttttacaa ttatggctat tcattgcttt cctaaagatt acggttgttc
     2821 agggacaaac ggctgttgac tactataaca acggcataag caagtatatg gcgaaagatt
     2881 atgccggcgc tgtagccgaa tacgacaagg ctatcgccat cgatgccggt tatgctatgg
     2941 catacaacaa caggggactg gcaaaataca agctcaagga ttatgcagga gccgaagccg
     3001 attataccca ggcgataaaa ttcaaacctg aatatgccat ggcatactta aaccgcggtc
     3061 tggccaggta taaccttaag gactttgagg gcgagctggc cgactacaac aagactatag
     3121 aactcgactc caaaatggct tcggcttact acaacagggc gcttgccaaa gaaagcatga
     3181 aagacagcga cggtgccctt gccgattatt taaaagccat agaatacgac ccaaaataca
     3241 gtaaaacagc atattataac atagcctcca tcaactacag ccgaaaggat tttaccggtg
     3301 ccataaaaaa ctttaccctg gccatagaaa acaatccgaa cgacgcccgt tcgtatttca
     3361 acagggggct ggcacattac gaattgggtg cctacgataa cgcaaaaacc gatttcacta
     3421 aaaccacaga actaaacccg cagcatgccg aagcatacta ctgcagggga ctgacaaaag
     3481 tttttatgga agacatacca ggagccatag ccgattacac caaaaccatt gaaataaatc
     3541 cccggcacga caaagcctat tacagccggg gggtggcaaa acaggaactc aaagaccatg
     3601 aaggcgccat cagcgatttt tcagccgccc ttaccatcaa ccctaaatat gctgaggcat
     3661 acaacaacag aggaactgca aaatataacc tgcacgactt caatggagcc atggaagatt
     3721 actctttggc catcgacaac aattcgcaat atgccatggc atattataac cgcgggttgg
     3781 taaaaattga tttgaaacag caggaatccg cctgcctcga tttcaaaaac gccctgaaat
     3841 atggagaggc agccgctaaa aaccagctaa gccggcactg cgaataaagt tcgtgacggt
     3901 tatcctctcg ttaccggtgt tttgaatttg tattttttaa acaccaggct tcttttatat
     3961 gcataatccc agatcgagca ataggaacgt agtgaactat tgcgcagtgg gtggcatcgt
     4021 gcgctgtatc tgggtaaccc cgacttagaa aatgtttccg ctatggcggt attacatttt
     4081 ctttgtcgat tacgcattaa cgaatcaaat catatgttat tgattattag aagataatga
     4141 ttgattagcc ctattgctac gcaatagaag tatttaatgc gtaatatggg gtaatgtaaa
     4201 aatttttctg attaaaattt ctgattaaca cagttataag ctaataatga ataattttgc
     4261 atacttttta aaccgacatg accaaacgcc ttatccttct ttttctgctt gcaggcaatt
     4321 atgcgatggc acagctaaac atcaatacct cgctgactgc caatcagtta atgcaaaatt
     4381 ttataggggg cggcgtaacg gtttttaatg tggtttatac cggcggtaac gcttcaaagg
     4441 cgactttcac caacggaaac aataccaacc tcggtcttag cgaaggcatt attttgtgtt
     4501 ccggcaatgc caccaatatt gccaacccgg ctactttttt tatgtcgaac aacctcaacc
     4561 aggcgggaga tgccacgctc aacagcatca ataatggcac cagtactttt gatgccgccg
     4621 ttctggaatt tgactttatc cctgtttgcg acagtgtaaa gttcaaatac gtttttggat
     4681 ctgaagaata tcctcactcc atctgttcgc aatacaacga tgtttttgca ttttttataa
     4741 ccggtcccaa tcctgcaggc ggaaactttg ccaaccataa tattgcttta atacccggaa
     4801 ccaacctgtt ggtttccgta aacagtgtca acctgggtgt tcccggcccc gggctttctc
     4861 cgtcaggctg cctgtcgctg ggttactcaa actattatgt ggacaacctc ggcatgggcg
     4921 gtaccaccat agcttttggc ggtttcacca ccccgctgac agctcgctgc aaagtgatga
     4981 aagacagcgt gtatcacatg aaaattgctg tggccgacgg gcaaaacgcc atctatgact
     5041 ccggtgtttt tctcgaagcc aacagttttt cgtctaatgt attaactgta aacacctgtt
     5101 atcccgacac catctttgga caaaacgcga tggaagggtg ttcggaagcc atagtttcat
     5161 ttattgcccc ggcaaatgtt acatccccca ccaccattac ctataccatt ggcggctccg
     5221 ccaccaacgg cgtggactat aacactgtcg gcaacagtgt tactattcct gcaggacacg
     5281 actccgccgg cctggtgata catcccctgg ccgacgaaaa caccgaaggc gccgaaaccg
     5341 tcattttaaa tatcacctat gactgtataa ccatcattga cactattact attattgata
     5401 aagtttcgct gcaggttact tccggcaatg atgccgctat ttgttcgggt aactcgctgg
     5461 tt
//
LOCUS       DAOSFP010000138         5442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13292_length_5442_cov_2.915166, whole genome shotgun sequence.
ACCESSION   DAOSFP010000138 DAOSFP010000000
VERSION     DAOSFP010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13292_length_5442_cov_2.915166"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3490)
                     /locus_tag="PLI16_05245"
     CDS             complement(<1..3490)
                     /locus_tag="PLI16_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH83999.1"
                     /translation="MVVYAVVFVLPAGLFTLVQSNLVQTYLARKVSAYLSEKLNTEVR
                     VDKVEIRFFADVVLKKVYVEDLHRNPLLSAEKINVQPRHIDLSSNYFELKKVVLENTV
                     FNLRKYKDEKKLNLQFVLDFFKSDKEKPAREKYPVKVVVDKLQLSGSGFSYINDNKDH
                     NNNGVMDFDDISLTHLDIDMNHVAVLADSVCADIKNLSVHEKSGFVIHNFSAQSIVSK
                     RNIVVKNLLAKTGNSSLDLDLTFAYEDFKSFNDFVHKVTIISAIRPTDLNFIDIAYFV
                     RQLHGMNDLIHFSSQVKGTVSNLKVKNFMMHYGADTYFDGDVTMNGLPQINETFINMK
                     VRNLTSSVSDLNAIVIPSANGEVVNLKLPLMLQRLGNINVNGVFTGFYTNFVSNATLN
                     SDLGTISTDIIVRNENKIISYEGEVSTENFYLGQLLDAGELLGKVSLVAQLGGSGLTA
                     DDADLIFQGNIDQVEFKGYNYSGISVNGTFAHKIFNGVIEVEDKNLEMVFNGEVDIND
                     SLPDFDFEADVAYDNLKMLKITPKDTISEVKGRVNIDFYGNNIDNLKGYITVNDASFR
                     QNKKNFDLRSFNLNTYISPTGYRTFDLKSDYVDAVFNGYFVFSELGNSFKKFILDYLP
                     KFYFALDTSNKNLARQDFNFDIRLYNATPLLDYLVGGLETSGNTIVKGNFNSLTNNAK
                     INAVSSRLSYKSFSLENWNLDFAAADGNINLLMNSDTLHMSDSAYLSNLNLDSRVLND
                     SIISLLQWDNKKATKRNAGKLNFYNYFQSNNQSVMGFKPSGLVVNDSVWTISHQGNIV
                     FDTGSIACNNLKLWYKEQNLTINGKISKNPADVLSVDFNSFDISNVDYLTNAKKFDLD
                     GVITGNVFISDLYNSPTYFADLKIKDFGVNYDKLGDASIQSKWDDIKKGVHIKAEIYY
                     VGNIGKSTPVMAEGYYYPGDKPQNFYIDISVENLRLKALSRYVASFGSIVSGTATGKL
                     YLRGKQKPELSGLLKVMRGALKINYLNTVYAFAYDSVKITKDAFTFNNLIAIDQPDLL
                     HREIDTVMVSGRITHENFKNIGFDINVEPRKALVMNTNAFMNELFYGKGYATGSAHIF
                     GDQNIINFDIAATTEKGTQLIIPVGNTSALQASEYVTFVTQGHQEDSVAQETGKIIKG
                     INLDMSFNVTDDAEVQL"
     gene            3538..4560
                     /gene="tsaD"
                     /locus_tag="PLI16_05250"
     CDS             3538..4560
                     /gene="tsaD"
                     /locus_tag="PLI16_05250"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOH84000.1"
                     /translation="MNTYILGIESSCDDTSAAVVNKNELLSNIIASQAIHKNFGGVVP
                     EYASRAHQINIISVIDQALHEANIQKNQLSAIAFTRGPGLLGSLIVGTSVAKSLGLAL
                     NIPLIDVDHIQAHMLAHFIQEKDSQGRAPGFPYLCLLISGGHTQIIMVKDFYTFETIA
                     NTIDDAAGEAFDKSAKILGLPYPGGQMIDQNAAAGNPLKYKFAEPRVKNDEFSFSGLK
                     TSFLYFVRDRLKENPDFITENLADLCASIQQTIINILAKKLIETSQRTGIRTVAISGG
                     VSANSALRKQLMSHAKAQGWELFLPKPVFCTDNAAMVAVAGYYKYLKKDFSGQDSTPY
                     TRYNTQ"
     gene            4671..5216
                     /locus_tag="PLI16_05255"
     CDS             4671..5216
                     /locus_tag="PLI16_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84001.1"
                     /translation="MKNLQLLSVIICASILFAACSGKKDDVKNNDLPATGVFSSNLEA
                     TGWMNQFTLSKDAAHSGRYSSRVDSVNQFSFGFKESVSIIGDTIPQKANIDFWVLYPQ
                     AGIKSTLVLSVDSCDKNIFWFGTPITDSVKTTAQWQQIKASVNLPENIMPTDKISVYV
                     WNNDKKTFYIDDFTVAFENKK"
BASE COUNT         1585 a         1195 c         1103 g         1559 t
ORIGIN      
        1 acaactgcac ctcggcgtca tcggtaacat tgaaagacat atccagattg atacccttga
       61 taattttacc cgtttcttgt gcaacactat cctcctgatg cccctgagta acaaaagtga
      121 cgtactcgct ggcctgcagg gccgacgtgt tgccaacggg aatgatcagc tgggttcctt
      181 tttcagtggt ggcggcaata tcaaaattga tgatattttg gtcgccgaaa atatgagcac
      241 ttcccgtggc ataacctttg ccataaaaca gttcgttcat gaaggcattg gtgttcatca
      301 ccagggcttt tcggggttcc acattgatat caaagccgat atttttaaaa ttttcgtgcg
      361 tgatacgccc cgataccatc acggtgtcaa tttcacggtg gagaagatca ggctggtcga
      421 tggcaataag gttattaaac gtaaaagcat cttttgtaat tttcacgcta tcgtaggcaa
      481 aggcatacac agtgttgagg taatttattt tcagggcgcc ccgcatcact ttgagcaagc
      541 ctgacagttc gggtttttgc tttcccctca gatacaattt gccggtagcg gtaccgctga
      601 caatagagcc aaaactggcc acatacctcg aaagagcttt cagccgtagg ttttccacac
      661 taatatcaat ataaaaattc tgtggcttat cccccgggta ataataacct tcggccatca
      721 ccggcgtgct ctttccgatg ttgccgacat agtagatttc cgcctttata tgtacccctt
      781 tttttatgtc atcccatttg ctttgtatcg aggcgtcgcc cagtttgtcg taatttaccc
      841 cgaaatcctt gatcttcaga tcggcaaaat acgttggcga gttgtacaga tccgaaataa
      901 atacgttgcc ggttatgaca ccgtcgaggt cgaatttttt ggcgttggtg agatagtcaa
      961 cattagaaat atcaaaggaa ttaaaatcaa cagacagcac atcagcagga tttttggaga
     1021 tttttccgtt gatggtcagg ttttgttctt tataccataa ttttagattg ttacaggcaa
     1081 tgctgccggt atcaaacact atgttcccct gatgactaat agtccacacc gaatcgttaa
     1141 ccaccagccc ggacggtttg aaacccatca ccgattggtt gttgctttgg aaataattgt
     1201 aaaaattcag cttgccagca tttcttttag tagctttttt gttatcccac tgcaaaagac
     1261 tgataatgct gtcattaagc accctggaat ccagatttaa attggaaaga taagcgctgt
     1321 ccgacatatg cagcgtatct gaattcataa gcaggtttat gttgccgtca gcagcggcaa
     1381 aatcgagatt ccagttttcc aacgagaaac ttttatagga tagccgtgaa gaaacagcat
     1441 ttattttagc attgttcgtc aacgagttaa aattcccctt aactatggta ttgcctgaag
     1501 tttcgaggcc gcctaccaga taatccagca agggcgttgc gttgtacaac ctgatatcaa
     1561 aattaaaatc ctgacgtgcc agatttttat tgctggtatc aagggcgaaa taaaattttg
     1621 gaaggtaatc gagtataaac ttcttaaatg agtttcccag ttccgaaaaa acaaaatatc
     1681 cattaaaaac agcatccaca taatcggatt tcaggtcaaa ggtgcgatat ccggtggggc
     1741 tgatataggt attaaggtta aaagaccgca ggtcaaagtt ttttttgttt tgccggaaag
     1801 aggcatcgtt gacggtgata tagcctttaa ggttatcaat gttgttgcca tagaagtcaa
     1861 tattcacacg acctttaacc tctgaaatcg tatctttggg ggtgattttg agcattttta
     1921 aattgtcata ggccacatcg gcctcaaagt cgaaatcggg cagcgagtcg ttgatatcca
     1981 cttcaccatt gaagaccatc tcaaggtttt tgtcttccac ttcgatcaca ccattgaaaa
     2041 ttttatgtgc aaaagtgcca tttacagaaa taccggaata gttataccct ttgaattcta
     2101 cctgatcaat gtttccctga aaaatcaaat cggcatcatc ggcagtcagc cccgagcccc
     2161 ccagttgggc taccaggctc actttaccca gcaattcgcc tgcgtcgagc aattgtccca
     2221 ggtagaaatt ttcggttgac acctcgcctt cgtaggatat gattttgttt tcgtttctca
     2281 cgataatgtc ggttgaaata gtgcccaggt cggaattcag cgtggcattg gatacaaaat
     2341 tggtgtaaaa tcctgtgaaa actccgttta cgtttatgtt tcccaggcgt tgcaacatca
     2401 atggaagctt caggttaacc acttccccgt tggctgaggg gataacgatg gcattcaggt
     2461 ccgacaccga tgacgtcaga tttctgactt tcatgttaat aaaagtttcg ttgatttgtg
     2521 gcagaccgtt catggtaacg tccccgtcaa aataagtatc agcgccataa tgcatcataa
     2581 aattcttcac cttgagattg ctcacggtgc cttttacctg tgatgaaaaa tgtataaggt
     2641 cattcatgcc atgcagttga cgaacgaaat aggcaatatc aatgaaatta agatcggtcg
     2701 ggcgaatggc tgagataatg gtaaccttgt gcacaaaatc attaaacgat ttaaagtcct
     2761 cataggcaaa ggtaaggtcg aggtcgaggc tggagttgcc ggtttttgcc aggagatttt
     2821 tcaccacaat gttgcgtttt gaaacaatag attgtgccga aaagttatga atgacaaatc
     2881 cgcttttttc atgcaccgaa aggtttttaa tatcggcgca tacagagtcg gccagcaccg
     2941 caacatgatt catatcaata tcaagatgcg taagagatat atcgtcgaaa tccataacgc
     3001 cgttgttgtt atggtctttg ttgtcgttaa tataagaaaa acctgaaccg gaaagttgca
     3061 gtttatcaac caccactttt acaggatatt tttccctggc aggtttttct ttgtcggact
     3121 tgaaaaaatc aagcacaaac tgcaggttaa gttttttttc atccttatac ttgcgtaaat
     3181 tgaagacagt gttttcaagg acaacttttt taagctcgaa ataattggat gacaagtcaa
     3241 tatgtcgtgg ttgtacatta atcttttcgg cactgagcag gggattccgg tgcaggtctt
     3301 caacatatac cttttttagc accacatcgg caaaaaagcg tatttccacc ttatcaaccc
     3361 taacctcggt gttaagtttt tcggataaat acgccgagac ttttcgggca agataggttt
     3421 gcaccaggtt gctttgcacc aatgtaaaca gtcctgcagg cagcacaaac accactgcat
     3481 aaacaaccaa taatatttta accgtttttt ttataccttt gaaaactttt tttcacaatg
     3541 aacacataca tcctgggcat agagtcatcc tgcgacgaca cttctgcggc agtggtcaat
     3601 aaaaacgaac ttctgtccaa cattattgcc agtcaggcaa tacataaaaa ctttggcggt
     3661 gtagtccccg aatatgcttc gagggcgcac cagataaata tcatctccgt tattgaccag
     3721 gcactccacg aagcaaatat acaaaaaaat caactgtctg caattgcttt tacccggggt
     3781 cccggtttgc tgggttcgct catcgtgggt acttcggtgg ccaaatcatt aggactggca
     3841 ctgaatatcc cccttattga tgtggaccat attcaggcac atatgctggc acattttatt
     3901 caggaaaagg acagtcaggg gcgcgcgccg gggtttcctt acctttgcct tttgatttcg
     3961 ggtggtcata cccaaattat aatggtaaaa gatttttata cctttgaaac cattgccaac
     4021 accattgacg acgccgccgg agaagcattt gacaaaagtg caaaaatact gggtcttccc
     4081 taccctggcg gacaaatgat agaccaaaac gctgccgccg gaaacccttt gaaatacaaa
     4141 tttgccgaac cccgtgttaa aaatgatgag tttagtttca gcggactcaa aacttccttt
     4201 ttgtattttg tccgcgaccg cctgaaagaa aatcctgatt ttatcaccga aaacctcgct
     4261 gatctgtgtg cctccataca acaaaccatc ataaacattc tggccaaaaa acttatcgaa
     4321 acttcgcaac gtaccggcat ccgtactgtg gccatttcgg gcggagtatc ggccaattcg
     4381 gctctacgaa aacaactgat gtcccacgcc aaagcacaag gttgggaact attcctgccc
     4441 aagccggttt tttgcaccga caatgcagct atggtggccg tagcaggata ctacaaatac
     4501 cttaaaaaag atttttcagg ccaggacagc actccctata cacgttacaa cacgcaatag
     4561 gtatcaaaca aaccaaaaat agtaaaagtt gatgctgcat tacgaaataa taattttttc
     4621 tatgctataa actcaggaaa ctatattttt gcaaaccaaa aaataaaaaa atgaaaaatc
     4681 tacaactctt atcggtcatt atttgcgcaa gcattttgtt tgctgcctgt tcgggaaaaa
     4741 aagatgatgt aaaaaacaac gatctgccgg caaccggagt tttttcgagc aacctggaag
     4801 ccaccggatg gatgaaccag tttacccttt cgaaagacgc cgctcactca ggcaggtatt
     4861 caagccgcgt ggattcggtt aaccagttta gttttggttt caaagaatcc gtaagtatta
     4921 ttggcgatac tatcccccaa aaagctaata ttgatttctg ggtattatat cctcaggcag
     4981 gcatcaaaag cacacttgtt ttaagcgttg attcctgtga taaaaatatt ttctggtttg
     5041 gcacaccgat caccgactcg gttaaaacca cagcccagtg gcaacaaata aaagcctcgg
     5101 tgaacctgcc cgaaaatatc atgcccaccg ataaaatctc tgtctatgtg tggaacaacg
     5161 acaaaaaaac tttctatatc gacgacttta ccgttgcatt tgaaaacaaa aagtaaccac
     5221 ttgtttgtgt tgaataattc aaagcaaaac ttaaaaagaa gctctgcagg gcttcttttt
     5281 taattcaaag gccgcccttc attgaaaata tctcgttatg gaacaaaaca attttgaagg
     5341 tgtttcctat ataaaaaaag atttcaccca aaacccgctg ccggctggga cttatgaaaa
     5401 ctgtattttc gatggctgcg atttttcaga caccaacctg aa
//
LOCUS       DAOSFP010000139         5428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13334_length_5428_cov_4.163224, whole genome shotgun sequence.
ACCESSION   DAOSFP010000139 DAOSFP010000000
VERSION     DAOSFP010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13334_length_5428_cov_4.163224"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="PLI16_05260"
     CDS             complement(<1..333)
                     /locus_tag="PLI16_05260"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HOH84002.1"
                     /translation="MTENKKIKFTARIIQHQNMDAAFVEIPFDVKEFFGRSGNLKVKA
                     LFDGKVVYRGSLFHMGQAAYVIGLTKEIRKRINKTFGDTVHVEMEEDKEERTVSLPED
                     VACLLAGNA"
     gene            complement(352..1275)
                     /locus_tag="PLI16_05265"
     CDS             complement(352..1275)
                     /locus_tag="PLI16_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HOH84003.1"
                     /translation="MDISFKALTACALCPRNCGANRFAGNSGYCRSGHEYAISSIVAH
                     RGEEPPVSGLHGIANVFFSHCNLQCIYCQNFQISSNLSAGNSKYHHFEKVLSDLHRLL
                     ESGCSAIGFVSPSHQIPQMVSIIKAFENYRPRPVFVYNSNGYDRVETLKELEGLIDVY
                     LPDMKYLDSSLASALSDAKDYPATARKALKEMYRQKGPVLHYLDENHVESGMIIRHLV
                     LPGYVENSLNVLRFIAEELSPKVHISLMSQYYPVYKAMKRTDINRPLKKQEYDLVVAE
                     AEKLGLTNGWIQEPESRDHYKPDFEHKHPFE"
     gene            complement(1284..2111)
                     /locus_tag="PLI16_05270"
     CDS             complement(1284..2111)
                     /locus_tag="PLI16_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84004.1"
                     /translation="MISGHVFGQSVPDDSTAQNRSWSFGINCGAAFANKYHANFYSGA
                     AGNENTLGYILDNKYHYDEIRQVINDTFSLYSMPAQMKYKPAFCVGFYVKKNLNNHLS
                     IFMQFNHSRFTTEGAFALKIGATSSSQTFDSTLTCPVWGSEDRINIDLGVGYEFPLDE
                     KISGFVEAGFNLNNTKVRENKIAIQSLEYSIINVYLDQQYIPNTQLQEYPVRQGGIGL
                     GAFLSPGIRFRFSEHLGLDLLGTIYWSKINLKHYAVFRPHYNVMVRFLFSSEFLKSN"
     gene            2265..2981
                     /locus_tag="PLI16_05275"
     CDS             2265..2981
                     /locus_tag="PLI16_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84005.1"
                     /translation="MKHLAVLFLCVLFSFSLSAQDTVQTTPQEKSPRVRLSGFGGLIS
                     ETFFLKNQVAEALGAGGALMINNYFFLGGYGLTTVSNHTIKDLIIPDEFLLDNRPTYY
                     YGKPLRINYGHAGIWVGGVFFPGKRVHFGVSSRFGWGNIHLTDSVNNSYINNVDYRLE
                     FTNDKIFVISPRIELEINITSWLKANIGLGYNFVTGIDFDRYKECKFSSPQLSIGIYF
                     GGFSDRDEEVNPEDDEYPEE"
     gene            3000..4172
                     /locus_tag="PLI16_05280"
     CDS             3000..4172
                     /locus_tag="PLI16_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOH84006.1"
                     /translation="MEKNLMMPMQNEYDKLRPYHDEEVNNAIGELIKDPEFAIFIRHL
                     LQGQDRNEVYSMLKGITSIEEFQRVFVLPVLDRIIKNSMDSLTCSGIDRVEKKQPYIF
                     ITNHRDIVLDSSFLNMLLFKYGYNTTQVAIGGNLLISPMITNLVRINKSFIVKRNIPP
                     RELYEYSKLLSTYIAHTLSERKESVWIAQREGRSKDGSDKTQYSLLKMLAMSYEGEER
                     DIFMKMNIFPVAVSYEYDPCDMFKAIELFSAANGGPYAKTKEADFQSMITGILGYKGR
                     VEVSFSNPLTDKDFDTLNELHGNDFLKGLASIIDNRIYSAYKLWDTNFIAYDLLNGSA
                     VFSDRYTVESKEKFINYLKEKSNKLDEFYPKRDLLNLILNMYSNPIKNKLSENLAI"
     gene            complement(4173..4625)
                     /locus_tag="PLI16_05285"
     CDS             complement(4173..4625)
                     /locus_tag="PLI16_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84007.1"
                     /translation="MNRKEIKKKLVDLCMQLHLETVERLTAEMNEAYQNAAEYGNPED
                     WLDTYKMDMLSKRDACSLKLQKVGEEMKMLERINPEKISDKAAFGSVIITETQKLFIA
                     VGLGKIVVDNETYFVISQSVPLFHAMKDHKAGDIFRFRDTEEKILEVF"
     gene            4879..>5428
                     /locus_tag="PLI16_05290"
     CDS             4879..>5428
                     /locus_tag="PLI16_05290"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOH84008.1"
                     /translation="MGKESKEIVQSRNVLEFITVANELCIFLEENDKYDIAFIYTYLQ
                     KVLPLLYLKGTMLPDIFVDDEDANERFVTLEQWEKIYLALLGKTATTDSFYFIGDDDG
                     SDNKPQKGSIAENLADIYQDLKDFILLYQKNMLAAQQNAVHACRVLFETNWGIKALLT
                     HTAIHNMLYGKKKSEKEFFLGFS"
BASE COUNT         1570 a         1145 c         1155 g         1558 t
ORIGIN      
        1 agcattaccg gccaacagac aggccacatc ttccggcagg gatactgttc gttcttcttt
       61 gtcctcttcc atttccacgt gcaccgtatc accaaaagtt ttattgatgc gttttcgtat
      121 ttctttcgta agcccgataa cataagctgc ttgccccatg tggaaaaggc ttccgcggta
      181 cacaactttt ccatcaaaca aagcttttac ttttaaatta ccgcttcttc caaaaaattc
      241 cttcacgtca aaaggaattt ccacaaaagc cgcatccatg ttttggtgct gaatgatgcg
      301 ggctgtgaac tttattttct tgttttctgt catgtaaata atgttaacag cttactcaaa
      361 agggtgctta tgctcaaaat ccggcttgta atgatccctg ctttccggtt cctgtatcca
      421 gccattggtc aggcccagtt tttccgcctc agccaccaca aggtcatatt cttgtttttt
      481 cagcggcctg ttgatgtcgg tgcgtttcat tgctttatac accgggtaat actgtgacat
      541 cagcgaaata tgtactttgg gcgaaagttc ttcggcaata aagcgtaaca cattcaggct
      601 gttctccaca tatcccggaa gcaccaggtg ccggataatc attcccgatt ccacatgatt
      661 ttcatccagg taatgcaaaa cgggaccttt ttgcctgtac atttctttga gtgctttgcg
      721 ggccgtcgcg ggatagtctt tggcatccga caaggccgaa gcaaggctgc tgtcgaggta
      781 tttcatgtcg ggaaggtaca cgtctatgag gccttccagc tctttcaggg tttcaacacg
      841 gtcgtatcca ttgctgttat atacaaaaac aggtcttggc cggtaattct caaatgcctt
      901 gataatagat accatctgcg gtatctggtg cgaaggggat acaaaaccaa tagcgctgca
      961 gcccgattcc aaaagacgat gcaaatcgga caagactttt tcaaaatgat gatatttgct
     1021 gttgcctgcc gaaagattag aacttatctg aaaattctgg caatagatgc attgcaggtt
     1081 gcagtggctg aagaaaacat tggcgatgcc atgaagcccg ctgacaggag gctcttcgcc
     1141 ccggtgtgcc acaatggaac tgatggcgta ttcgtggccc gatctgcaat agccgctgtt
     1201 gcctgcaaac ctgttggccc cgcaattgcg cggacaaagg gcacaagctg tcaaggcttt
     1261 aaaagagata tccattgatt aggtcagttg gattttagaa attcgctgga aaacaaaaag
     1321 cgaaccatca cattataatg aggacggaac acggcataat gtttcaggtt gatttttgac
     1381 cagtaaattg ttccaagcag atcgaggccc aggtgttcac taaaccggaa tcgtatgccc
     1441 ggcgacaaaa aggcacccaa cccaataccg ccttgacgca caggatattc ctgaagttgc
     1501 gtgttgggta tatattgctg gtctaaatac acgttgatga tgctgtattc gagactttgg
     1561 atggcaattt tgttttccct gactttggtg ttgtttaaat taaaccctgc ttctacaaaa
     1621 cctgatattt tttcatcgag aggaaattcg taacccacac ccaggtcaat attgatacgg
     1681 tcttccgaac cccataccgg gcaagtgagc gtgctgtcaa aggtttgtga cgatgaagtg
     1741 gctccgattt tcagggcaaa ggccccttcg gtggtaaacc gcgaatggtt aaactgcatg
     1801 aaaatgctca ggtggttatt caggtttttt ttaacataaa atcccacaca aaaggcaggc
     1861 ttgtacttca tctgcgcagg catgctgtac aatgaaaaag tatcattgat cacctggcgg
     1921 atttcgtcgt agtgatattt gttgtctaaa atataaccca gggtattttc gttgcccgca
     1981 gcgccgctgt aaaagttggc atgatactta ttggcaaaag ccgccccgca gttgatgcca
     2041 aaactccagg agcggttttg tgccgtggaa tcatcaggta cgctttggcc aaaaacatga
     2101 ccgcttatca tgctgaaaaa aattgctaaa aaactgaaaa cgtaaatttt tatcgtcctg
     2161 tccatactta taaaatacct gctgcgttaa tagtgataca caaaattaag tcttttggtg
     2221 atttttataa caaagttttt tattaatttg caaaaaagtt tttcatgaaa cacctggcgg
     2281 tattgttttt gtgtgtcctg ttctcttttt ccctgtctgc gcaggatacg gtacaaacaa
     2341 caccgcagga aaaaagtccc agagtgcggc tgagcggttt cggaggattg atttctgaaa
     2401 catttttttt gaaaaatcag gttgcagagg ccttgggcgc cggtggcgcg ttgatgatta
     2461 acaattattt ctttttaggc ggatacggcc tgacaacagt ttcgaaccac acaataaaag
     2521 acctgataat tccggatgaa tttctgttgg ataaccggcc gacgtattat tatggaaaac
     2581 cattgcggat aaactacggc catgccggta tatgggtggg cggagttttt tttcccggca
     2641 aaagggtcca ttttggtgtt agctcacgat ttggctgggg caatatacac ctgaccgatt
     2701 cggtaaacaa ttcctatata aacaatgtgg attaccgact ggaatttacc aacgacaaga
     2761 tttttgtaat ttcgccgcgt atcgaactgg aaattaacat cacttcatgg ctgaaagcca
     2821 atattggatt gggttataac tttgtaacag gcattgattt tgacagatac aaagaatgta
     2881 agttcagctc gccgcaattg tccataggaa tatatttcgg aggttttagc gaccgcgatg
     2941 aggaggtaaa tcccgaggat gatgaatatc ctgaagaata aaaacagtgt gaataatgaa
     3001 tggaaaaaaa tctgatgatg ccaatgcaaa atgaatatga taaattgagg ccataccacg
     3061 acgaggaagt caataatgcc ataggcgaac tgatcaaaga tcccgagttt gctattttta
     3121 tacgtcattt gttacaagga caggatagga atgaggtcta tagtatgctg aagggcatca
     3181 cgtcaattga agaatttcaa cgggtgtttg tgctgccggt gcttgaccgg atcataaaaa
     3241 acagcatgga tagcctgacg tgttcaggaa ttgaccgtgt cgaaaaaaaa caaccctata
     3301 tttttatcac caaccaccgc gatatagtcc tggattcttc gtttttaaac atgttgcttt
     3361 tcaagtatgg ttacaatacc acccaggtag ccataggggg taacctgctg atatcgccca
     3421 tgattaccaa tcttgtccgt atcaataaga gttttattgt caaacgcaac attccgcccc
     3481 gcgaactata cgaatattca aaactgcttt ccacatatat agcccacacc ttgtccgaac
     3541 gaaaagaatc tgtgtggata gcgcaacgcg aaggccgttc gaaagacggc agcgacaaaa
     3601 cccagtacag cctgctgaaa atgctggcca tgagctacga aggtgaggaa agagacattt
     3661 ttatgaagat gaatattttt cctgttgccg tttcttatga atacgacccc tgcgatatgt
     3721 ttaaggccat tgaactgttt tcggcggcca atggcggccc ttatgcaaaa accaaagaag
     3781 ccgattttca gagcatgatc accgggatac tcggttataa gggcagggtg gaggtttcgt
     3841 tcagcaaccc attaacagat aaagattttg atactctgaa tgaactccat ggcaatgatt
     3901 ttctgaaagg cctggcctcc ataattgata accgaatcta cagcgcatac aaattgtggg
     3961 ataccaactt tatcgcctac gacctgctca atggcagcgc cgtattcagc gacagataca
     4021 ccgtggaatc aaaagaaaag tttattaatt atctgaagga aaaatccaac aagctcgatg
     4081 aattttatcc taaaagggat ctcctgaacc tgatactcaa tatgtattca aacccgataa
     4141 aaaacaagtt gtcggaaaat cttgccatat aatcagaaca cctccagtat tttttcttcc
     4201 gtatcacgaa acctgaagat atcgccggcc ttgtggtctt tcattgcgtg aaacaagggc
     4261 acggactgcg aaataacaaa ataggtttcg ttgtcgacca caatcttgcc aaggcctaca
     4321 gcaataaata atttctgggt ttcagtgatt atcaccgacc caaaggccgc tttatccgat
     4381 atcttttccg gattaatccg ctccagcatc ttcatttctt cacccacctt ctgtagcttc
     4441 agcgagcagg catcacgctt gctgagcata tccattttgt aggtatccag ccagtcttcg
     4501 ggattaccat attccgcagc attttgatag gcttcattca tttcggcagt tagcctttca
     4561 actgtttcca gatgcaactg catacacaaa tccactaatt ttttctttat ttccttcctg
     4621 ttcattttgt tcaacttgtt ttgttcggca ggaatgatat actacctgat ccggaaatgt
     4681 tcctgagtaa aaaataaaat aaaatttaaa tgcaaaaaaa gcgtttacta aactgatatg
     4741 atcagttatg gattattatt ctgttttttt gtaaaaaaca aatatatatg ttttttctgc
     4801 aaaaagcaaa tttttaaaaa aaattgcaat taataagtaa tagaaattat atttgttttc
     4861 tcaaaacatt ttttgtctat gggtaaagaa tcaaaagaaa tagttcagtc gcgcaacgtg
     4921 ctggaattta ttacggtagc caacgaattg tgtatttttc ttgaggaaaa tgacaaatac
     4981 gacatcgcat tcatctatac gtatctgcaa aaagtattgc cgttgctgta cctgaaaggt
     5041 accatgctcc ccgacatatt tgtggatgac gaggatgcca acgagcgctt tgttacgttg
     5101 gaacaatggg aaaagattta tctggcacta ttaggcaaaa cagccacgac cgacagtttt
     5161 tattttattg gcgacgatga tgggagtgat aacaaaccgc agaaaggtag tattgccgaa
     5221 aacctggcgg atatctacca ggacctgaaa gattttattt tgctctacca gaagaatatg
     5281 ctggcagccc agcagaatgc cgttcatgcc tgcagggtat tgttcgaaac caactggggc
     5341 atcaaagctt tgttgaccca taccgctatc cataatatgc tttatggtaa aaaaaaaagt
     5401 gaaaaggagt tctttttagg gttttcac
//
LOCUS       DAOSFP010000140         5422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13354_length_5422_cov_4.508664, whole genome shotgun sequence.
ACCESSION   DAOSFP010000140 DAOSFP010000000
VERSION     DAOSFP010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5422
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13354_length_5422_cov_4.508664"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLI16_05295"
     CDS             <1..455
                     /locus_tag="PLI16_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84009.1"
                     /translation="PVIVVHNSTKIDKGFKNIIFPISTTADVRQKVKWTILIAKTFKS
                     KIHLFQLNQSSAEDKKTMQHYIRQITNEFDKEGIKYQHILADKKTSFATQLQEYAHEV
                     KAELITIMITIDLLNFKYGPYDEKMMFNPYEIPVMCINPIKTKIIYWK"
     gene            547..1311
                     /locus_tag="PLI16_05300"
     CDS             547..1311
                     /locus_tag="PLI16_05300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH84010.1"
                     /translation="MEHKLSVVIITFNEEQNIERCLTSVKDIADDIVVVDSYSTDNTE
                     AICKKAGARFFQRQWAGYSEQKNYGNSLALFDWILSLDADEALSDDLKKSIISIKKDL
                     TAPDFRICRITNYCGKWIRHSGWYPDIKVRLFDRRDHQWEGLIHERLNFQNEKAIPVL
                     KGDCYHFSYYSREAHIAQARHLSELVALDLFNKKKNVCLLKQKFAPSMKFIKMFFINF
                     GFLDGTAGYTIALISAKAVTIKYEKLRTLYQEEKNK"
     gene            1268..2347
                     /locus_tag="PLI16_05305"
     CDS             1268..2347
                     /locus_tag="PLI16_05305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOH84011.1"
                     /translation="MKSSEHFTRKKKINKRNPRIIISRTDNIGDVILALPMAGIIKKA
                     IPGAYIMFLGKSYTRDIIEMSEHVDEFIDWESIKEQEPELQIQSFKKLHANYIIHALP
                     VRQIAHLAKKAGIRKRIGASGRIFHWQYCNKIIFFTRRRSKLHEAQLNLKLLRALGVK
                     RPFPLQDIVKNYGIIPPNTLPEHYRDQIDNNRFNLILHPKSKGSAREWEMQNYFELIS
                     LLPKDKFKIFITGTSADYDLIQAEIINNNPGVINLAGKFSLLELISFIANTDGLVACS
                     TGPLHIAAALGKFAIGIFAPIKPMHPGRWAPLGANASVLALNKKCSKCRGNKHCECIQ
                     SITPDLVKNKLMEVFKPDPACVDII"
     gene            2419..3204
                     /locus_tag="PLI16_05310"
     CDS             2419..3204
                     /locus_tag="PLI16_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84012.1"
                     /translation="MNKNFFAIITILLFVCSNLFAGEITLKGNYYGFNLQVLNPSNGN
                     GFCVTEVLVNNQKTQDEVNSNAFEIDFSLLNLKIGDPVTVVIKHHNGCVPEVINPRAL
                     QPTGNVSFITPKADKTGHINWGIKGDLMDEPLIIEQFRWNKWTKVGEFAPTDTVKKNM
                     YSFEFIPHTGLNQFRIFFNDVNGNPVYSKIIKYTSRNPEITLLSAKTSDKLTFSADTQ
                     YEIFDLKGNFISEGFGKEIDVTDLEKGKYWLNYDNKSVNFTKK"
     gene            complement(3236..4078)
                     /locus_tag="PLI16_05315"
     CDS             complement(3236..4078)
                     /locus_tag="PLI16_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH84013.1"
                     /translation="MKNNVLKGKRGVIFGAVNNNSIAWKTAEHAYNEGARFVLTNMPA
                     AMRMGNLSELAEKCRAEVIAADAGNVEDLKNLFTKAPEILGGKIDFVLHSVAMSYNVR
                     KNNPYDNVDYDLFLKTIDVSALSLHKMIQTAVKLDALNDGASVVALSFLAAHRTSHGY
                     NDMAEAKAMLESIARSLGYVLGKKKKIRINTVSQSPILSKATSGLDFMEAVIDFSESI
                     SPLGNADADSCADICVMLFSDYTRYVTMQNIFNDGGFSSMAMNSDIADMMRCAKNKET
                     DPVV"
     gene            4214..>5422
                     /gene="gpmI"
                     /locus_tag="PLI16_05320"
     CDS             4214..>5422
                     /gene="gpmI"
                     /locus_tag="PLI16_05320"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HOH84014.1"
                     /translation="MNSKKLLLIIMDGWGEGEKNHANAIYTAQTPFIKGLYKNKDIAY
                     CRLDASGLAVGLPDGQMGNSEVGHLNFGAGRIVYQDLVRITKSAEDNTLAQNRVLCQA
                     YEYARKENKNVHFIGLISDGGVHSLQTHLYKLCDIAKDYGLDNVFIHAITDGRDTDPR
                     SGLGYLKELQEHLASSTGKIATLIGRYYTMDRDKRWERIKIGYDLMVSGTGKKSTDVL
                     RAVQESYDENITDEFIKPVVFTDNNGNPLTTIQNDDVVICFNFRTDRLREITTVLTQK
                     DMPEAGMKTMPLHYLTMTRYDESFQNIHIIFEPQDLKKTLAEVLSNLGKKQLRIAETE
                     KYPHVTFFFSGGQETPFVGEDRIMVPSPKVATYDLQPQMSAPEVTEKAVEAINKNYYD
                     FVCLNFANADM"
BASE COUNT         1738 a         1293 c         1012 g         1379 t
ORIGIN      
        1 cacccgtcat tgtggtacac aattcaacca aaatcgacaa aggctttaag aatatcattt
       61 tcccgataag tactaccgcc gacgtgcgtc aaaaggtcaa atggaccatt ttaatcgcaa
      121 aaacctttaa atcaaaaatt cacctcttcc aactgaacca gagttcggcc gaagacaaga
      181 aaaccatgca gcattatatc cggcagatta ctaatgaatt tgataaagaa ggcataaaat
      241 atcagcacat tctggcagat aaaaaaacct cttttgcaac tcaactgcaa gagtatgccc
      301 acgaggtaaa agccgaactg ataaccataa tgattaccat cgatctgctc aattttaaat
      361 acggacctta cgacgaaaaa atgatgttca acccttacga aataccggta atgtgcatca
      421 atccaattaa aaccaaaatt atttactgga aataaaatca ttttttagtt ttacactctg
      481 caggcctttg taaacatgtt gttgaagtac attttttatg attagggtag tatttcgtta
      541 ctgattatgg aacacaagct atctgtagta ataattactt ttaacgaaga acagaatatt
      601 gaacgctgcc ttacttccgt taaagatatt gccgacgata tagtggtggt tgattcctat
      661 tccactgata acaccgaagc catttgcaaa aaagccggag cgcgtttttt tcagcgccaa
      721 tgggccgggt acagtgagca aaaaaattac ggcaattctt tggctttgtt cgactggatt
      781 ctttctctcg atgccgacga agccctgtct gatgatctca aaaaaagcat tatcagcata
      841 aaaaaagatt taacagcccc cgattttcgc atttgcagga ttaccaacta ttgcggaaaa
      901 tggatacgcc acagtggctg gtaccccgac attaaagtcc gccttttcga ccgccgggac
      961 caccaatggg aaggccttat tcacgaaagg ctgaattttc aaaacgaaaa agcaatccct
     1021 gttctaaaag gtgattgtta tcacttcagc tattattcgc gcgaagcaca catcgcccag
     1081 gcccggcatt tgtcggaact cgttgccctg gacctcttta ataaaaaaaa gaacgtctgc
     1141 ctcttaaagc aaaaatttgc cccatcgatg aagtttatca aaatgttttt tattaatttt
     1201 ggatttcttg atggcacggc aggatatact attgcactca tttctgcaaa agctgttaca
     1261 ataaaatatg aaaagctcag aacactttac caggaagaaa aaaataaata aacgcaaccc
     1321 acgcatcatt atcagccgta ccgacaatat cggcgatgtg atccttgccc tgcctatggc
     1381 cggaattatt aaaaaggcta ttcccggcgc atatattatg tttcttggca aatcatacac
     1441 ccgcgatatt attgaaatga gcgaacacgt tgatgaattt atcgactggg aatccataaa
     1501 agagcaagag ccggaactac agatacagag ttttaaaaaa ttacacgcca attatatcat
     1561 ccatgcctta ccggtacgcc agattgcgca cctggccaaa aaagccggca tacgcaaacg
     1621 tatcggagcc agcggacgaa tttttcactg gcagtattgt aataaaatta tttttttcac
     1681 ccgccgcaga tcaaagctcc atgaagcaca gctaaacctc aaacttctca gggctctcgg
     1741 cgtaaaaagg ccatttccgt tgcaggacat cgttaagaat tatgggatca tcccgcccaa
     1801 caccctgccg gaacattatc gcgaccagat cgacaacaac cggttcaacc tcatattgca
     1861 cccaaaatca aaaggcagcg cacgggaatg ggaaatgcag aattatttcg aacttatcag
     1921 cctgttgcca aaagacaaat tcaagatttt catcaccggc acatctgcgg attatgacct
     1981 cattcaggct gaaataatca acaataaccc cggcgtaatt aatcttgcag gaaaattttc
     2041 gcttcttgaa ctcatcagct tcatagccaa taccgacggc ctggtagctt gcagcaccgg
     2101 tccgctgcac attgccgccg cgctgggaaa atttgccatc ggtattttcg cacccataaa
     2161 accaatgcac ccgggacgtt gggcgccctt gggagccaat gcctcggtgc tcgcactgaa
     2221 caaaaaatgc agtaagtgca gaggaaacaa acactgtgaa tgcatccaaa gtatcactcc
     2281 cgatttggta aaaaacaaac tgatggaagt ttttaaaccc gaccctgctt gtgttgatat
     2341 tatctgaaaa cttttatcgg gttgacgtca ctcttcatcc aaaaataatt aatattgcac
     2401 cctgtgttct atttgactat gaataaaaat tttttcgcca taatcaccat cctgttgttt
     2461 gtttgcagca acctgtttgc aggcgaaata acccttaaag gaaattatta cggattcaac
     2521 ctccaggtgc ttaaccccag caacggcaat ggtttttgcg ttaccgaggt gttggttaac
     2581 aaccaaaaga cccaggacga agtcaattct aacgcctttg agattgactt ttcgctgtta
     2641 aacctaaaaa taggcgaccc ggtaacagtg gtgattaaac accataacgg ctgtgtgccc
     2701 gaagtaatca atccaagggc cttacaaccc accggcaacg tgtctttcat cactcccaaa
     2761 gccgacaaaa ccggccatat aaactggggc atcaaaggcg acctcatgga tgaacccctg
     2821 attatcgaac aatttcgctg gaataaatgg actaaagttg gcgaatttgc accaacagac
     2881 accgtgaaaa aaaatatgta ctcctttgaa tttattccgc atacaggact gaaccaattt
     2941 cgcatttttt tcaatgatgt caacggaaac ccggtgtatt cgaaaattat caaatacact
     3001 tcgcgcaacc ctgaaatcac acttttatct gcaaaaacct ccgataaact gacattttcg
     3061 gcagataccc agtacgagat attcgacctt aaaggcaact ttatttctga aggttttggc
     3121 aaggaaatag atgttaccga cctcgaaaaa ggcaaatact ggctgaacta cgataacaaa
     3181 tcggtaaatt ttacgaaaaa ataaaccccg gcaattaaac tgcagataaa tcctgctata
     3241 cgactggatc cgtttcctta ttcttcgcgc atcgcatcat gtcggcaata tcgctgttca
     3301 tagccatgct agaaaaccct ccgtcgttaa atatattctg catggtcaca tagcgcgtgt
     3361 aatccgaaaa caacatcaca caaatatccg cgcatgaatc cgcatcagca tttccgagag
     3421 gagaaatgct ttctgaaaaa tcaataacag cctccataaa atccaatccg ctggtagctt
     3481 ttgacaatat gggcgattgc gaaacggtat tgatacgtat ttttttcttt ttgccaagca
     3541 cataccccaa acttcgtgca atagactcaa gcatcgcctt ggcttcggcc atatcattat
     3601 agccatggct ggtacgatga gccgcaagaa aagataaggc aaccacagaa gccccatcgt
     3661 tcagcgcatc cagctttacc gccgtctgaa tcatcttatg caacgacaat gcagaaacat
     3721 caatggtttt caaaaataga tcataatcta cattatcata aggattgttt tttctgacat
     3781 tatatgacat agcaacagaa tgaagcacaa aatcaatttt tccgccaagg atctcaggtg
     3841 ccttcgtaaa aaggtttttc aaatcttcta catttcctgc atcggcagca ataacctccg
     3901 ccctgcactt ctcagccagc tccgaaagat tacccatacg catggcagcc ggcatattgg
     3961 taagaacaaa acgggcaccc tcattatagg catgttcggc ggttttccat gctattgaat
     4021 tattattaac agcgccaaaa ataacacccc gttttccttt taaaacatta tttttcatag
     4081 ataaaaaatc aaaattacgc tgcaaaaata caaaaaacgg caataacgga tagcaatatt
     4141 tacacttctg ttacccatgc tcctcctcaa taaaaatcag tatctttgca tattatttca
     4201 accattatct caaatgaact caaaaaaact tttacttatt atcatggatg gctggggcga
     4261 aggagaaaaa aatcatgcca atgctattta cactgcacaa actcctttta ttaaaggatt
     4321 atataaaaat aaagatatcg cctattgccg gctcgatgct tcagggcttg ctgttggcct
     4381 tcccgacgga caaatgggaa attcggaggt aggacatctg aattttgggg ctgggcgaat
     4441 agtatatcag gatctggtgc gcattacaaa atctgccgaa gacaatacct tggcacaaaa
     4501 cagggtgctg tgtcaggcat acgaatacgc ccgcaaagaa aacaaaaacg ttcatttcat
     4561 cggtttgata tccgatggcg gggtgcactc gctgcaaacc catctgtata aactctgcga
     4621 tattgccaaa gattacggcc ttgacaacgt gttcatccat gctatcaccg atggtcgcga
     4681 caccgacccc cgcagcggcc tgggttattt aaaagaactt caggaacact tagcctcttc
     4741 taccggtaaa atagcaacgc tcataggacg ctattatacc atggaccgcg acaaacgctg
     4801 ggaacgaatc aaaatcgggt acgatctgat ggtcagcggc accggaaaaa aatcaacaga
     4861 cgtactgcgt gccgtgcagg aatcctacga tgaaaatatc acagatgaat tcatcaaacc
     4921 ggtcgtcttc actgataata atggcaaccc gctcaccact atacaaaatg acgatgtggt
     4981 gatatgcttc aattttcgca ccgaccgcct ccgcgaaatc accacggttc tgacacagaa
     5041 agacatgccc gaagcaggca tgaaaaccat gccgctgcat tatctcacca tgacccgcta
     5101 cgatgaaagt ttccaaaaca tacacatcat ttttgaaccc caggacctaa aaaaaaccct
     5161 ggcagaagta ctgagcaatc tcggaaaaaa acaactacgc attgccgaaa cagagaaata
     5221 tcctcacgtt acttttttct tctcgggtgg acaagaaacc cctttcgttg gcgaagaccg
     5281 cattatggta ccctcgccca aagtggccac ctacgatctt caaccacaaa tgagtgcccc
     5341 ggaagttacc gaaaaagccg tagaagccat caataaaaac tattatgatt ttgtttgtct
     5401 gaactttgcc aatgccgaca tg
//
LOCUS       DAOSFP010000141         5379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13509_length_5379_cov_3.997558, whole genome shotgun sequence.
ACCESSION   DAOSFP010000141 DAOSFP010000000
VERSION     DAOSFP010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13509_length_5379_cov_3.997558"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="PLI16_05325"
     CDS             complement(<1..621)
                     /locus_tag="PLI16_05325"
                     /inference="COORDINATES: protein motif:HMM:NF019145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84015.1"
                     /translation="MRYADHNVNPTFIRYNPQNEPLFQELDSWLKTTFNIKSNMGYKL
                     ISTEKDKLGFIHYRYRQTADGIPVRDAVFIVHVYQDKIHAFNGIIYSEVNISNAFSIT
                     GQEALSRALSYVNAEEYMWQKSSEEAWIKKLKNNPQATFYPVAEKEIIYSEKEKAFRA
                     AYKFDVYASRPVSRQHIYVDAGNGNIVRAFKKLYGTDVPATAVTKYS"
     gene            982..1677
                     /locus_tag="PLI16_05330"
     CDS             982..1677
                     /locus_tag="PLI16_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84016.1"
                     /translation="MKKLVTFCVVITLLFSHAAGQSTGTIRGKVFDESNNEALFGAMV
                     VVTTGQANIGCQTDPDGYYTIKPLNSGIYTIKISSMGYQTTIVKDIIVNPGKITFVED
                     TYLGNIGITLEDSVAVIRDFRDPLIRPDPVTIIRPEEYQDIPGKRDLSGVISKLNSDV
                     YSDEQGELYFRGARNDNFVYIVDGVKSMDNQAHIPSGAIGSIAIYTGGVPANYGDFTG
                     GCVVIETKSFFSR"
     gene            1714..2967
                     /locus_tag="PLI16_05335"
     CDS             1714..2967
                     /locus_tag="PLI16_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84017.1"
                     /translation="MQKLLTAMNRSLTIVIAILILAHCYVPGQEVFTIKGYVVDSLTL
                     LPVENAEIVTEGFTLKTSPDKNGFFSFVSPVSECWIEVSCLSYNTKKCKVSGKALSEI
                     RISPKSYDLPSVEIRERKPLDLMPGMSYQVMDYEFAGENIILLAYERQSIFLPKLLLI
                     NRQGDTLDVLTVSRPEKLCRDFDEKVCFLTKTSCYELETGNNEISLHYQGSREDFEQI
                     NKTIVSREGSVFYLRQFLDNNQTLNYYTYQNETDQLNCFRNISDRSAINANRRGVYFD
                     GKEEDLRFQELIINRPVFAPLFMIEDTMLLFNFLESKLEKYSVETGNPQEIGETSIAL
                     HHSKGFVNNMIIDHARHKIYGIFREKGISKLKEICPSDGTISQIFEIQDFIYIENIKI
                     NDGVVYFLYKSKDLSEYKKLYTMKI"
     gene            3092..3538
                     /locus_tag="PLI16_05340"
     CDS             3092..3538
                     /locus_tag="PLI16_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH84018.1"
                     /translation="MKKVVFIFISVILISVSTFSQNKGPAIKFDYTEYDYGTIYQGGD
                     GSCDFKFKNTGDEPLVLSSVRSSCGCTVPSWPKDPILPGHSASIKVTYDTKRIGTISK
                     QIIVISNAVSEPSLTLAIKGNVLQKPNEILPEKQVNQGFTPTPNAN"
     gene            3574..3990
                     /locus_tag="PLI16_05345"
     CDS             3574..3990
                     /locus_tag="PLI16_05345"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH84019.1"
                     /translation="MKKISLIFALVMLSVFVAGAQTSTTQTAATKVAGPEITFENLEH
                     DYGTITQGANGDCVFKFKNTGNEPLILSDVRKSCGCTTPSWSKEPILPGQSGEIKVGY
                     NTNNVGTFSKTITVISNATNANITLIIKGTVNAKAN"
     gene            4096..5292
                     /locus_tag="PLI16_05350"
     CDS             4096..5292
                     /locus_tag="PLI16_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOH84020.1"
                     /translation="MPKISTKGLAMPASPIRKLVPYADEAKRKGRKVYHLNIGQPDIE
                     TPHGAMDVLKNTPIKVVEYSHSAGILSYRKKLVEYYKHWGIDVNENQMIVTCGGSEGI
                     LFAFMACLDPGDEVIIPEPFYANYNGFAVTAGVVVKPVISNIENGFALPPVAEFEKLI
                     TPKTKAILICNPNNPTGYLYSKEELLELRELIIKHDLYLFSDEVYREFCYEGAEHFSV
                     MNMDGLDNNVVLLDSVSKRYSACGARIGVFITRNKEVYNVAMKFAQARLSPPTFGQVL
                     SEAAIDAPQEYFDNVMAEYIARRNIVVEAINKMPGCFCPNPKGAFYAVARLPIDDSDK
                     FCQWLLEDFEYNNQTVMLAPATGFYSTCGLGKNEVRISYVLKVEDLKAAMVCLAEALK
                     VYPGRK"
BASE COUNT         1696 a         1137 c         1087 g         1459 t
ORIGIN      
        1 gctgtatttg gtcactgccg ttgcaggaac atcggtaccg tataattttt tgaatgcccg
       61 cactatattg ccattaccgg catcgacata aatatgctgt ctggaaacag gccggctggc
      121 gtacacatca aatttataag ccgcgcggaa ggccttttct ttttccgagt aaatgatttc
      181 tttttcggca acaggataaa atgtagcttg cggattattt ttcaacttct ttatccaggc
      241 ctcttcgctg cttttctgcc acatatattc ctctgcattt acataagaaa gggcacgcga
      301 taacgcttcc tgtccggtaa ttgaaaatgc attgcttatg ttgacctcgc tgtaaattat
      361 tccattaaac gcatgaattt tatcctggta cacatgaaca ataaaaacag cgtcgcgcac
      421 cgggatgcca tctgctgttt gcctgtaacg gtaatgaata aatcccagtt tgtccttttc
      481 ggtgcttatg agcttatagc ccatattgga tttgatatta aaagtggttt tgagccagga
      541 gtccagctcc tgaaacaatg gttcattctg cggattatac cttataaatg taggatttac
      601 attatggtcg gcatagcgca ccaggttgct ccctataata tgattttgag cgttaatacc
      661 tcaaatacct gtgaaaatcc aacaacagat ataaaggtcg aaatggcaaa taccgcaagg
      721 atttttttca taactgaatt ttataatata acaatgttgt gtaaaagtaa gcaacttagt
      781 tgattttttt atcgcttaac taatttttaa ccaagctgct atcagcaaaa tttctttgta
      841 accgttaact tccgttttta tgccggaaac ttttttcagg ataccatttg ctgacaacgc
      901 tccgaaacag ttcccgatgt attggaaatt tagtacaaca aaatccaata aatatagtac
      961 taacccacaa aatcaaaacc catgaaaaaa ctagtaactt tttgcgtagt cattacgctt
     1021 ctgttttccc acgccgcagg gcaaagcacg gggactatcc ggggtaaagt gtttgatgaa
     1081 agcaacaatg aagcgctgtt cggagctatg gtggtggtaa ccaccggcca ggccaacatc
     1141 ggctgccaga ccgatccaga cggctattac actattaagc cgctgaattc aggcatttat
     1201 accattaaga tttcatctat gggataccag accaccattg tcaaagacat cattgtaaat
     1261 cccggcaaga ttacctttgt ggaagacacc tacctcggaa atatcggcat aacgctggaa
     1321 gattctgtgg ccgttatccg tgatttcagg gacccgttga tcaggcctga cccggtgaca
     1381 attatcagac ccgaagaata tcaggacatc cccggaaaga gagacctgtc gggagtcatc
     1441 agcaaactga actccgatgt ttatagcgac gaacagggtg aattgtattt caggggagcc
     1501 agaaacgaca attttgttta tattgtagat ggggtaaaat caatggacaa ccaggctcac
     1561 ataccttcag gagccattgg cagcatagcc atttacaccg gcggcgtacc tgccaactat
     1621 ggtgatttta ccggaggatg cgtagttata gaaaccaaaa gttttttcag tcgttgacac
     1681 aaataatgat tacgaaaacg cacctgctgt gtagtgcaga aacttttaac agccatgaac
     1741 cgaagcctga ccatagtaat agccatactt atacttgcac attgttatgt gcccggtcaa
     1801 gaggttttca ctataaaggg ctacgttgtc gattcgctca ccttgctgcc agttgaaaat
     1861 gcagaaatcg taactgaagg ttttacatta aaaaccagtc cggacaaaaa tggctttttc
     1921 tctttcgtgt cgcccgttag tgaatgctgg atagaggtaa gctgtctttc atacaacacc
     1981 aaaaagtgca aggtgagcgg aaaagcactt tctgaaataa gaataagtcc caaatcatac
     2041 gacctgccat cggtcgaaat aagggagagg aaacctcttg acctgatgcc gggcatgagt
     2101 tatcaggtga tggattacga attcgcaggt gaaaacatca ttttgcttgc ctatgaaaga
     2161 cagagcatct tcttgccgaa actgctcctt atcaaccgtc agggcgacac gcttgacgta
     2221 ctgacggtca gcaggccgga aaaactttgc agagattttg atgaaaaagt atgtttctta
     2281 accaaaacaa gttgttatga gctggaaacc ggcaacaacg aaatcagcct tcattaccag
     2341 ggaagcagag aagatttcga acaaataaac aaaactattg taagcaggga gggctccgta
     2401 ttctatttgc gacaatttct ggacaacaac cagacattga attattacac ttaccaaaat
     2461 gaaaccgacc aattaaactg cttccgcaac atcagcgaca ggtctgctat caacgccaac
     2521 cgccgcgggg tatattttga cgggaaagaa gaagacctca ggtttcagga attgataata
     2581 aaccggcctg tgtttgcccc tcttttcatg atagaggaca caatgctcct tttcaatttt
     2641 ttggaatcaa aactggaaaa atacagtgtg gaaacgggaa atccgcagga aatcggagag
     2701 acaagtatcg cactgcacca cagcaaagga tttgtaaaca atatgataat tgatcatgcc
     2761 cggcataaaa tttatgggat tttcagggaa aaaggaattt cgaagctaaa agaaatctgc
     2821 ccctcggatg gcactatttc gcaaatattt gaaattcaag attttattta cattgaaaac
     2881 ataaaaatca acgatggagt agtatatttt ttatacaaat caaaagatct ttcggaatac
     2941 aagaaattgt acacaatgaa aatataattg acaataccca ataaacgcca attattttcg
     3001 tttaggtttt ttgattttgg cattatattt gaaaccgttg tgataaaata aaaaaatttt
     3061 cgtaattttg tttttcaaat tcatgttgtg tatgaaaaag gtagtattca tttttatcag
     3121 cgtaattcta atttccgtca gcactttttc gcaaaataaa ggccctgcca taaagtttga
     3181 ttatacggaa tatgactatg gcaccattta tcagggcggc gatggcagtt gtgattttaa
     3241 gtttaaaaac accggtgatg aaccgctggt attatccagt gttcgttcat catgtggctg
     3301 tactgttcca agctggccca aagaccccat tctgccggga cacagcgcat cgataaaagt
     3361 tacctacgac acaaaaagga taggcactat cagcaaacag atcattgtga tttccaatgc
     3421 ggtttctgaa cccagtctga cattggccat caaaggcaac gtactgcaaa aacccaatga
     3481 gattttgcca gaaaaacaag tcaatcaagg ttttacgccc actccaaatg ccaactaata
     3541 aacaaataaa taaccaacca aatatcaaat aaaatgaaaa aaatatcttt aattttcgca
     3601 ttagtaatgt tatcggtttt tgttgccggt gcacaaacat ccaccaccca aacagcagca
     3661 actaaagttg caggtccgga aatcactttc gagaacctgg aacatgatta tggcactatc
     3721 actcagggag ccaatggaga ctgtgttttt aaatttaaaa atacgggcaa cgagcccctc
     3781 attctttctg atgtaagaaa gtcatgcggc tgtaccaccc cttcatggag caaagagccc
     3841 atactgccgg gtcagagcgg agaaataaaa gttgggtaca acaccaataa cgtaggtact
     3901 tttagcaaaa cgataaccgt aatatccaac gcaaccaatg caaatattac tcttatcatc
     3961 aaaggaacgg tcaacgccaa agccaactaa gaaactttgc aacaaaaaga aataatattt
     4021 cttttatcca aaaaaaagtt aaacatttgt accggtaatt tattaccggt ttttttattt
     4081 taaaacaaaa acattatgcc aaaaatttca acaaaaggcc tggccatgcc ggcgtcaccc
     4141 atccgaaaac ttgttcccta tgccgacgaa gcaaaacgca aaggacgcaa agtttaccat
     4201 cttaacatcg gacaacccga tatcgaaact ccccatggtg ccatggatgt attaaaaaac
     4261 acccctatca aagtggtgga atacagccat tctgcaggga ttttgtcgta ccgcaaaaaa
     4321 cttgttgaat attacaaaca ttggggtatt gatgttaacg aaaaccagat gatagtaacc
     4381 tgcggcggct ccgaaggtat attgtttgcc tttatggctt gcctcgatcc gggcgacgaa
     4441 gttattatcc cggaaccttt ttacgccaat tacaacggat ttgccgttac agccggtgtg
     4501 gtggtaaaac ccgtgatatc aaacatcgaa aatggctttg cattgccccc tgttgccgaa
     4561 tttgaaaaac ttatcacgcc aaaaaccaag gccattctca tctgcaaccc caacaaccct
     4621 acgggatact tatattccaa agaggaactg ttggaattac gcgaattaat aatcaaacac
     4681 gacctgtatc tgttttctga tgaggtgtac cgtgaatttt gctatgaagg agcagaacat
     4741 ttttcggtta tgaacatgga tggcctcgac aataatgtgg tgctgctcga ctcggtatcg
     4801 aaacgttaca gtgcctgcgg tgccagaata ggcgtattta tcacccgtaa caaagaagta
     4861 tataatgttg ccatgaaatt tgcccaggca aggttaagcc cgcccacttt tggccaggta
     4921 ctttcggagg cagctatcga tgcgccgcag gaatattttg acaacgtgat ggcagaatat
     4981 atcgcgcgca gaaacatcgt tgttgaagcc attaacaaaa tgcccggctg tttttgtccc
     5041 aaccccaagg gagcttttta tgccgtagca cgtctgccta tagatgactc tgacaaattt
     5101 tgccagtggc tgcttgaaga tttcgaatat aacaatcaga ctgtcatgct ggcccctgcc
     5161 accggttttt attccacctg cggcttaggc aaaaatgagg tacgtatcag ttatgtgttg
     5221 aaagttgagg atctgaaagc tgccatggta tgtctggcag aagcattgaa agtttatccc
     5281 ggaaggaaat aatatccggg aatacaaaga caatccgcga tttgtaaata gtcgctcctt
     5341 aaaatagtta taaacaattg aatgtcaata acatgtagt
//
LOCUS       DAOSFP010000142         5366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13557_length_5366_cov_4.963095, whole genome shotgun sequence.
ACCESSION   DAOSFP010000142 DAOSFP010000000
VERSION     DAOSFP010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13557_length_5366_cov_4.963095"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLI16_05355"
     CDS             complement(<1..540)
                     /locus_tag="PLI16_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH84021.1"
                     /translation="MNVKIIWCILIVNTLFAAGRNYSFAQNDPVIVVFGDTRTKVSGN
                     VSTNHTVNQSVCKKIMHYNPVAVFHTGDIVYNGLKKSAWATFKNFFAPIIDSSHFYPC
                     YGNHELRAKTVATDFALPNNGKWYSVDIGKCHFVIIDNFSDYSKGSEQYQWVENDLKN
                     CSSNKFCLVIMHLPVFSSGP"
     gene            complement(559..1101)
                     /locus_tag="PLI16_05360"
     CDS             complement(559..1101)
                     /locus_tag="PLI16_05360"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOH84022.1"
                     /translation="MIIAKDGDVVLRELTKSDIPKLAEYANNEKVSINLRDAFPSPYT
                     QEDARQFIAMVDRQQPKTFFAIAYKDQYAGNISLLPGADVYKKSAEIGYFIGEPFWNM
                     GIMTKAVRLITDYGFKHFDLVRIHTGVFEYNKASQRVLEKCGFIKEGVSSKAVFKNNH
                     FYDEIRYALLNDAYNAIQKP"
     gene            complement(1235..2989)
                     /locus_tag="PLI16_05365"
     CDS             complement(1235..2989)
                     /locus_tag="PLI16_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84023.1"
                     /translation="MKHFNFKLIAVLFLAAAMFTGCGLGKMIKKYPTVKYTVTPEVLE
                     THGGKISVGVSGTIPPKYFNKKASVTITPVIKSMEGTTLATLKSITLKGEKATGEGQT
                     IKYKEGGSFSYSDVVNYTENMNMSELSIGPLATKGKKVDVPLGTNKLADGVIYTSTRI
                     EREEDFQTAEHGYELETIITKKGNLYFDYNKSNLNLSMPLNKDENNKAMLNDTLMNFL
                     GLNWKIKSININAWASPEGELTLNQKLSDERGVTAKKWFTEKIKKMIKDKKLSGITKA
                     EELQVLVNSRGEDFDGFMKALNASNIPQKQTIANVIQSQASKAEREKSIKDMTVIYSE
                     IENMLSVLRRGEFVIDCYLPKKTKEEIIMLSTTSPDKLDNKELLYSATLTEDVATKYL
                     IYKAATTLYPNDWKGFCNAGNSALQLGKLDEAKNYLEKANQLNPNNGQINYNLGVLSF
                     WNKDDAAAQTYLNNAKSKGIDVSHNEALLKIRQGDYAGAISSFGGKKCKYNLALAQLL
                     SGNNTAAKQTLDCTKPQTAAVYYLTAVNAARTSNTAVMYEYLTKAVQADSKYRKEARE
                     DREFLKYFKEAAFQDAIK"
     gene            complement(3134..3670)
                     /locus_tag="PLI16_05370"
     CDS             complement(3134..3670)
                     /locus_tag="PLI16_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH84024.1"
                     /translation="MRKTKNTELNRLSVEAFKEAKKNQIAVVLDNIRSMNNIGSVFRT
                     CDAFRVEKLLLCGITATPPHREIHRTALGATESVDWEYCETTRQAVTVLKEKGFLVVA
                     IEQTDTSISLEEYFPPASKKMAFVFGNEINGIDEEVLDLCDYSVEIPQFGTKHSINVS
                     VSAGIILWDIVTKMRKTE"
     gene            complement(3745..4668)
                     /locus_tag="PLI16_05375"
     CDS             complement(3745..4668)
                     /locus_tag="PLI16_05375"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOH84025.1"
                     /translation="MKKVLLIFFLMAGSLLYAQEKIETVAKPMDRAAGDSVKAWTVLA
                     TGALNVTQAAFSNWAAGGENSIGLGALANIKADYKKGKHSWKNNLGLAYGFQFLGKGS
                     NARFTKTDDKIEYTTSYGYAVSKHWDVTFLANFRTQFVNGYNYPNDSVAISKFMAPGY
                     LVAGIGMNYVPAEYFQLYMSPAAGRFTFVTDQTLANACAFGVDSAKHIKGEFGPYIRA
                     VFNKEVFKNVTLNTSLELFSDYLKDFGNVDVNWNVLIGMKVNKWLSATIQTQLLYDDD
                     IMIKSTPSSDPGPRTQFKEVIGVGLSYTFNH"
     gene            complement(4697..5119)
                     /gene="mscL"
                     /locus_tag="PLI16_05380"
     CDS             complement(4697..5119)
                     /gene="mscL"
                     /locus_tag="PLI16_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOH84026.1"
                     /translation="MGIVKEFKEFAVKGNMMDMAVGIIIGAAFGKIVSSLVNDIIMPP
                     LGKLLGNVNFTDLYYAMDGNVYESLVKAKEAGAPVFAYGNFIQTVIDFLIIAFAIFMI
                     IKGMNSLKKKKEEAPAAPPAPTKEETLLTEIRDILKNK"
BASE COUNT         1416 a         1053 c         1157 g         1740 t
ORIGIN      
        1 tgggcccgaa ctgaacaccg gcaggtgcat aattaccagg caaaatttgt ttgaagaaca
       61 gttttttagg tcgttttcta cccattgata ttgttcggaa cctttcgagt aatccgaaaa
      121 attatcgata atcacaaaat gacatttgcc aatatccacc gaataccatt tgccgttgtt
      181 gggcagtgca aagtcggtag ccacggtttt tgcacgcaat tcatgattgc cgtaacaggg
      241 ataaaaatgg ctgctgtcaa ttatcggtgc gaaaaaattt ttaaaggtcg cccaggcaga
      301 ttttttcaac ccgttgtaca caatatctcc ggtatggaaa acggccacag ggttgtaatg
      361 cataattttt ttacatacac tctggttgac agtatgattg gtgctgacat tgcctgaaac
      421 cttggtgcgg gtatcgccaa aaacaacaat aacgggatcg ttttgcgcaa aactgtaatt
      481 cctcccggcc gcgaacaatg tattgacaat aaggatacac cagattattt ttacattcat
      541 tttatttatt ttttttgttt agggcttttg aatagcgttg taagcatcat tcaacagggc
      601 gtaccttatt tcatcataaa aatgattgtt tttaaaaaca gccttgctgg aaacgccttc
      661 tttgataaac ccacattttt ccaacacccg ttgcgatgct ttattatatt caaacacccc
      721 ggtatgaatc cttaccaggt cgaaatgttt aaatccataa tcggtaatca accggacagc
      781 ttttgtcatg atgcccatat tccagaaagg ttcgccaata aaataaccaa tctctgcact
      841 ttttttgtac acgtcggctc cgggcaataa actaatattt ccggcatact ggtccttata
      901 cgctatggca aaaaaagttt tgggttgttg ccggtcaacc atcgctataa attgcctggc
      961 atcttcttgc gtataaggcg aaggaaaagc atcgcgcagg ttgatgctca ctttttcgtt
     1021 gttggcatat tcggcaagtt tgggaatatc tgattttgtc aactcgcgca aaacaacatc
     1081 gccgtctttt gcaattatca tggttggttt ttttttgtta aagtacaaaa atatgttttc
     1141 ctgtctggtg tttcaaaata atttggtatt agataaccag caaaaaaaaa ctccgggaga
     1201 taattccgga gttttttcag gtgttatcag atacttattt tatagcatcc tggaaagcag
     1261 cttccttgaa atatttcagg aattctctgt cttcgcgggc ttcttttctg tatttggaat
     1321 cggcctgcac tgctttggtg aggtattcat acataacagc ggtgtttgat gtacgggcag
     1381 cgttaacagc ggtcagataa taaacagcag cggtttgcgg tttggtgcag tcgagagtct
     1441 gttttgcagc ggtgttgtta cccgaaagca actgagccag agccaggttg tatttgcatt
     1501 ttttgccgcc aaatgagctg attgccccgg catagtcgcc ttgtctgatt ttgaggaggg
     1561 cttcgttgtg gctgacatca ataccttttg atttggcatt gttcagatag gtttgggcgg
     1621 cggcatcgtc tttattccag aatgaaagta cgccgaggtt gtagttaatc tgtccgttgt
     1681 tggggttaag ttgatttgct ttttccagat agtttttagc ttcgtcgagt ttgccaagtt
     1741 gcagagcgct gtttccggcg ttgcagaaac ctttccaatc gttgggatac agggtggtgg
     1801 cagctttgta gatgagatat ttggtggcta catcttcggt aagggtagca gaatataata
     1861 attctttgtt gtcgagtttg tcgggagagg tggtggagag catgatgatt tcttctttgg
     1921 tcttcttggg caggtagcaa tcaataacaa attcgccgcg gcgcaaaaca gaaagcatat
     1981 tttcaatttc gctgtagata acggtcatat ctttaatgga tttttctctt tcggctttgc
     2041 tggcctggct ttggataacg ttagcaatgg tttgtttttg aggaatgttt gaagcattga
     2101 gggctttcat gaaaccgtca aaatcttcgc ccctggaatt gaccagaact tgtaattctt
     2161 cggccttggt aataccggac aattttttgt ctttgatcat ttttttgatt ttttcagtaa
     2221 accatttttt tgcggtaacg ccacgttcat ctgaaagttt ttggttaagg gtcaactcgc
     2281 cttcgggtga agcccaggcg ttgatgttga tactttttat tttccagttg agaccgagga
     2341 aattcatcag ggtgtcgttg agcatggctt tgttgttttc gtctttgttc aacggcatgc
     2401 tgaggttaag atttgattta ttgtaatcaa aatacaggtt tccttttttg gtgatgatgg
     2461 tttccagttc gtagccatgt tctgccgttt gaaaatcttc ttccctttca atacgggtgg
     2521 aggtgtaaat aacgccatca gccagtttgt tggttcccaa tggaacatct acttttttgc
     2581 ctttggtggc caggggtcct attgataatt ccgacatgtt catgttttcg gtataattca
     2641 ccacatcgct atatgaaaaa cttccacctt ctttgtattt tattgtctga ccttcgccgg
     2701 tagctttttc tcccttcagg gttattgatt tcagggtagc cagggtggtt ccttccatgc
     2761 ttttaattac cggagtaatg gttaccgaag cttttttgtt aaaatatttc ggaggaatag
     2821 taccgctaac accaacggaa atttttccgc cgtgtgtttc aagtacttcg ggggttaccg
     2881 tgtattttac tgtcgggtat ttcttgatca ttttgcctaa cccacaaccc gtgaacattg
     2941 ctgctgccag aaataaaaca gcaattaatt taaagttgaa atgtttcata aaatatattt
     3001 ttaaacgtta ataaaagaaa cctattgatt ttggcctaca aatataaaaa agtatttaac
     3061 acatcggaaa aaaaaattaa aaaatgcttt tgactatggt tgaaaaatgt cattagccgg
     3121 ttatttttgg tttttactct gttttccgca tttttgttac aatatcccaa agaattattc
     3181 cggcgctgac agaaacgttt atggaatgtt tggtgccaaa ctgtggtatt tcaacggaat
     3241 aatcgcacaa atcgagcact tcttcgtcta taccgtttat ttcattgcca aaaacaaaag
     3301 ccattttttt agaggccggg ggaaaatatt cttccaggct gatgctggta tccgtttgtt
     3361 cgatggccac gacgagaaat cctttttctt ttaatacggt tactgcctgc cgggtagttt
     3421 cgcagtattc ccagtcaacg gattcggtgg cgccgagagc cgttcggtgt atctcgcggt
     3481 ggggcggcgt ggcagtgatg ccgcacaaca atagtttttc caccctgaag gcatcgcagg
     3541 tacgaaatac cgagccaatg ttgttcatac ttctgatgtt atccagcact acggcaattt
     3601 gatttttttt tgcctcttta aatgcctcca cgctaagccg gttcagttcc gtattttttg
     3661 tctttctcat aatggaaatt tgtcccaaaa ataaaaaaac ctcacgttaa cacatgaggt
     3721 tttttattaa tgcggtgtgt tttcctaatg gttaaatgta taggaaagcc ctactccgat
     3781 tacttctttg aattgtgtac gggggcccgg atctgacgat ggcgttgatt taatcataat
     3841 gtcatcatcg tacagcagtt gtgtctgaat agtagccgac agccatttgt ttactttcat
     3901 gccgatgagc acattccagt ttacgtccac atttccgaaa tctttcagat aatccgagaa
     3961 cagttccagc gaggtattta gcgttacatt tttaaacact tccttattga agacggcacg
     4021 gatgtatggg ccgaattcac ccttgatgtg ttttgccgag tccacgccaa aagcacaggc
     4081 attggccagt gtctgatcgg taacaaaggt aaatcttccg gcggcaggcg acatatacag
     4141 ttggaaatat tcggcaggaa catagttcat accgataccg gccaccaggt aacccggcgc
     4201 catgaatttg gatatagcca cactgtcgtt aggatagtta taaccattaa caaactgtgt
     4261 gcggaagttt gccaaaaaag ttacatccca atgtttcgaa acggcatagc cataagaggt
     4321 ggtgtattca atcttgtcgt cggttttggt aaatcgggca ttgctgcctt ttcccagaaa
     4381 ctggaatccg tatgccaggc ccaggttgtt tttccatgaa tgtttacctt ttttgtagtc
     4441 ggctttgatg ttggcaagcg ccccgaggcc aatagagttt tcaccgccgg cagcccagtt
     4501 tgagaaagct gcctgggtaa cattcagcgc accggtggca aggacggtcc atgctttaac
     4561 agaatcgccg gcagcccggt ccattggttt tgctacagtt tcaatttttt cctgtgcata
     4621 cagcaatgaa ccggccatca ggaaaaaaat caatagtact tttttcatgt ctaaaaaaat
     4681 tttattaata caaaaactac ttgtttttca gaatgtcgcg gatttcggta agcagtgttt
     4741 cttccttggt aggtgcagga ggagctgcag gcgcttcttc ttttttcttc ttcagactgt
     4801 tcatgccttt tataatcatg aatatggcaa aagcgataat caaaaagtcg atgactgtct
     4861 gaataaaatt accataagca aaaacggggg ctccggcttc tttagccttg acaaggcttt
     4921 cgtaaacgtt gccatccatg gcatagtaca agtctgtaaa attgacgttg cccagcagtt
     4981 ttcccagcgg gggcatgata atatcgttaa ccaacgagct gacgattttg ccgaaagcgg
     5041 caccaattat gataccaacg gccatgtcca tcatattgcc ctttacggca aattctttaa
     5101 attcttttac gatacccata gttttaagtt tttaagatta tgaatgcaaa attaatgaaa
     5161 gatttgattt taaaacataa tttaagaaat atttaaaaat aaaaaagtta taaaaataaa
     5221 taaatgtgta caaattttta attcgctaaa agagaataat tagaaatctt tgtcgggatt
     5281 tgtctgagca ggatgtgaaa accaaagagg atttatcaga aaaaaggggg gggcacactg
     5341 gatcgctttt tcgcttacac taaaat
//
LOCUS       DAOSFP010000143         5333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13680_length_5333_cov_4.002653, whole genome shotgun sequence.
ACCESSION   DAOSFP010000143 DAOSFP010000000
VERSION     DAOSFP010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13680_length_5333_cov_4.002653"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLI16_05385"
     CDS             complement(<1..187)
                     /locus_tag="PLI16_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOH84027.1"
                     /translation="MNGDPAVYETLKTLNIPFEYLEHPPAPTIEIAKQYWQGHDAQHC
                     KNLFFRNHKGNRHYLVIF"
     gene            312..743
                     /locus_tag="PLI16_05390"
     CDS             312..743
                     /locus_tag="PLI16_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84028.1"
                     /translation="MKKLSFVIFIMVLTFSAYTQNTTFDTISIERRFLSANLDYQGKN
                     LSLMKLEDLCKPYADANDELLQAKRNNNPALIMTLVGGVLIGYSGIKWLSDGDLPWYY
                     AAGGVVLIGCTIPLYLGSRNHSINAARIYNYEMRHKQPITP"
     gene            1036..1977
                     /locus_tag="PLI16_05395"
     CDS             1036..1977
                     /locus_tag="PLI16_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH84029.1"
                     /translation="MSISRKIISHIKDYFTFNKSERRGLLVLCILIVLLIMFNFFAPR
                     FVARQKYDSSRFEREVAEYLKTQEVISKKPKTFQKKDPFNVFDANLSAVEQKLSPFVF
                     DPNTLPYEGFVEMGFTEKQALTISKYRAKGGRFRKKEDFKKIYSISDKEYTVLEPFIE
                     IKSFADGQQQQMEEPKKTEPAVLVDFNTATEEELKKINGIGDYFAEQIVKYRDRLGGF
                     NAKEQLLEVPKMDSAKYAKISPFVELNLNAVRRINVNKATFDELKAHPYIGYNIALSL
                     INYRTKHGNFATVDDIKKSALITDKNFPKISYYLCTK"
     gene            2056..2604
                     /locus_tag="PLI16_05400"
     CDS             2056..2604
                     /locus_tag="PLI16_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOH84030.1"
                     /translation="MKKVAISLIAIIVLSGFTYAQKNRNKPVVFKWYTFQEALKLNKK
                     NPKKIFVDIYTDWCGWCKKMDAGPFADSVIKAYMAQHYYPVKLNAESKDTIEFKGNTY
                     VNPNPSAARSSHQLAAALLQGRLSYPSFVILNEEFEILNTIVGFKSAKELEPILHYFG
                     ENAYEKLPYNKFFETFTGSVAP"
     gene            2803..3957
                     /locus_tag="PLI16_05405"
     CDS             2803..3957
                     /locus_tag="PLI16_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH84031.1"
                     /translation="MKAAKINVLFVSSWYPTRDKPTAGNFVQKHAEAAALYASVDVLH
                     VAFDRSLKTKFEYQKEEKNNITSHLVYIRPPGIKCSLIKTIKYIKAYFYGYKMIYGHQ
                     GKPDIIHANVLMPVGLIAFLLHLYKKVPYVITEHWTGYLPQDPNKPGWRLCLFRFFAK
                     KAARLMPVTAHLVNAMKKSGIKGNYTVVPNVVDTRLFLPANAQTDKVKRIIHVSSLLD
                     QQKNFSGILLALSLLAEKRDDFVLEVVSDGDFEQFARKYQKLNISHKLFFHGRKNTGE
                     VAAMMQRADFLLLFSNYENFPCVIAEAMACGLPVLSSEVGGIAEHIGPARGLLTEAQN
                     IDALVLQLDQMLDTCRSYDKNAIRAYAEKHFAYKVVGKQFLEIYNQVICS"
     gene            3948..5243
                     /locus_tag="PLI16_05410"
     CDS             3948..5243
                     /locus_tag="PLI16_05410"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOH84032.1"
                     /translation="MFLKIFKTFGVRLSSAIISLLIAVVISQFLGAGGKGEQGIIITT
                     IALILIFANMVGGASLVYLTPKLPLKKLILPSYVWSFFISALFVGVLFLFPLIEAKYV
                     WHVALLTLLNSFTSVNTSVLLGKENIRAVNIINFSQVALTISALLVFFISGIATNIYG
                     YIYSLYFAYGCTLVMSVILLSPYRHKSPGDEPVSYTYAIRMLFRYGFMNQLAHITQLL
                     SFRVSYYFLESFQGYDAVGIYSNGVSITESVWMISGSVALVQYSKISNTGDKKFNQQL
                     SAELLRISLLLSFLVLIPLVLFPGSFYAFIFGKDFGDINRIMWLLAPGVLVFVNALIL
                     GHYFSGTGQYHINSIGSAIGLVVTIILALVLIPRMGYLGAALTASLSYLSTTVFVTIC
                     FCRESKLKLIDLLPLPRYFSGYYLSVKQYLCKNKAARDE"
BASE COUNT         1523 a         1070 c         1140 g         1600 t
ORIGIN      
        1 cgaaaatcac caggtaatga cggttgcctt tgtgattgcg gaaaaacagg tttttacaat
       61 gctgcgcatc gtggccttgc cagtactgtt ttgctatttc aatggtagga gcagggggat
      121 gttcgaggta ttcgaagggg atatttagtg ttttcaaggt ttcgtaaacc gcaggatcac
      181 cgttcataaa gaatattttt tcgcaaaaat agtcaacctg gttttcattt gccacaataa
      241 aacaattttg caggagttca gtcaaataaa atccttaatt ttgttttgat ttctttgtaa
      301 tccctactgt tatgaaaaaa ctttcattcg taatttttat catggtcctg actttttctg
      361 cctacaccca aaataccaca tttgatacta tttctatcga aagaaggttt ttaagtgcca
      421 acctggatta tcagggaaaa aacctctcgt tgatgaaact cgaagaccta tgcaaacctt
      481 atgctgatgc caatgatgaa ctgttgcagg ccaagcgtaa caataaccct gccttgatta
      541 tgactctcgt aggaggagtg cttattggtt attcgggcat caagtggctg tcggacggcg
      601 atttgccctg gtattatgca gccggcggcg ttgtgttgat tggctgcacc attcccttat
      661 atttaggttc gcgaaatcac agcatcaatg ctgcgcggat atataactac gaaatgaggc
      721 acaaacagcc tattacaccc tgaagcctta atttgttttc ataaatattt ttgcggtatt
      781 ttgtttgccg gattttatcc tgcatacata catgcccgcg ggaagatgtt tcacatcatt
      841 attcactatt tactgcttat tggttaagga aagaatgcat ccttgatgtt ttgcgcctac
      901 acgcagatca ggctgcaaaa aagcgcaaac aggaaaaata tttttttcat aaaaagatat
      961 tttgaatttt tatttacaat aaaataacga cataaataaa ttatatataa ttttacagaa
     1021 aaaaatgaaa cagtggtgag tataagtaga aaaattatca gccacattaa ggattatttc
     1081 acttttaata aaagcgaacg cagagggttg cttgttttgt gcatattgat tgtgttgctc
     1141 atcatgttta atttttttgc tccccgcttt gtggctcgac aaaaatatga ttcttcccgt
     1201 tttgagcggg aggtcgccga atatctgaaa actcaggagg taatttccaa aaaacctaaa
     1261 accttccaga aaaaagatcc cttcaatgtt tttgatgcca atctctcagc tgttgaacaa
     1321 aaactgtctc ctttcgtctt tgatcccaat acactgcctt atgaaggttt tgttgaaatg
     1381 ggatttaccg aaaaacaagc gctgaccatc tcgaaatacc gtgcaaaagg cggtagattc
     1441 aggaagaagg aggattttaa aaagatttat tctatcagtg acaaagagta cactgtgctg
     1501 gaacctttta tcgagataaa atctttcgcc gatgggcagc aacaacagat ggaagaaccc
     1561 aaaaaaacgg agccggcagt tttggtcgat ttcaacacgg ctacagagga agaactaaaa
     1621 aagattaacg gaataggtga ttattttgct gaacagatag taaaataccg tgaccggcta
     1681 ggaggtttca atgcaaagga acaactgctt gaggtgccaa agatggatag tgcgaagtac
     1741 gcaaagataa gccctttcgt agagttaaat ttgaatgcag tgcgaaggat aaatgttaac
     1801 aaggccactt ttgatgaact gaaagcgcat ccttatattg gttataatat tgcgctgtcg
     1861 cttatcaatt accgcactaa acacggaaat tttgccacag tagatgatat aaaaaaatcc
     1921 gctcttatca cggataagaa ttttccaaaa atttcatact atctttgtac aaaataattc
     1981 aatttccaaa tagcgggttt ttaatacaaa caagaccttt ctatcaaagg tttatcatct
     2041 aatcagaaga aaaacatgaa aaaagttgcg atttcactca tagccattat tgttttgtca
     2101 ggcttcactt atgcacaaaa aaacagaaat aaacctgttg tgtttaaatg gtacaccttt
     2161 caggaggcgt tgaagctgaa taaaaagaat cccaaaaaaa tttttgtgga tatatacaca
     2221 gactggtgcg gctggtgcaa aaaaatggat gccggccctt ttgccgacag tgttataaag
     2281 gcatatatgg cgcagcatta ttatcctgta aaactcaatg ccgaaagtaa agatacaata
     2341 gagtttaaag gtaataccta tgttaatccc aatccctcgg cggcacgttc cagtcatcag
     2401 ttggcagccg cactgttgca aggccgcttg tcttatcctt cttttgtaat cctcaacgaa
     2461 gagtttgaga ttctgaatac cattgtaggt tttaaatctg ccaaagaact ggagcccata
     2521 ctgcactatt tcggcgaaaa tgcctatgaa aagctacctt ataacaagtt tttcgaaact
     2581 tttacgggtt cagtagcccc ataagaaaaa cggtggcttc gagagcctca accaccggga
     2641 aggggtagcc tcagccaccg ttgaaaaaac cgctgcctga ggttctcgaa ggcagcgaac
     2701 aagatgctct cgaaggcagc gaaaaacttc tgaaacagca aaaaaaaaca ttttatgaaa
     2761 attagaatca taaaaatacg tttttaacgt acttttgcat ttatgaaggc agccaaaatc
     2821 aacgtgttat ttgtttccag ctggtatccc acccgggata aacctacagc cgggaatttt
     2881 gtccaaaaac atgccgaagc agccgccctt tatgcatctg tggacgtgtt acatgtagcc
     2941 ttcgacagat ccctgaagac gaaattcgag taccaaaaag aagaaaaaaa caatataaca
     3001 tcgcaccttg tttacatcag gcctcccggg ataaaatgtt ctttaattaa aaccataaaa
     3061 tatattaagg catattttta tgggtataaa atgatatacg gtcatcaggg gaagcccgat
     3121 attattcatg ctaatgtgct gatgccggtg ggtttgatag cttttttgct tcatctttat
     3181 aaaaaggtcc cctatgttat caccgagcat tggacaggtt acctgccaca agatcccaat
     3241 aagcctggat ggcggttgtg tctgtttcgt ttttttgcca aaaaggcagc acgtttgatg
     3301 cccgtaacgg cccatttggt gaacgccatg aaaaaatccg gtattaaggg taactatact
     3361 gttgtgccca atgtggttga tacccgcctt tttttgccgg ctaatgcgca aaccgacaag
     3421 gtaaagcgca tcattcatgt gtcttctttg ctcgatcagc agaaaaactt cagtggcatt
     3481 ttgttggcgc tttcgctgct ggccgaaaaa cgcgatgatt ttgtactgga agtggtcagc
     3541 gatggcgact ttgagcagtt tgcccgtaag taccaaaagc tgaatatttc acataagctg
     3601 ttttttcatg gcagaaaaaa caccggagaa gtagctgcaa tgatgcagcg ggcggacttt
     3661 ttgctgcttt tcagtaatta cgaaaatttt ccctgtgtga ttgccgaggc tatggcttgc
     3721 ggactgcctg tattgtcgtc ggaggtggga ggcatagctg aacatatagg tccggctcgc
     3781 ggactgctta ccgaggccca aaatatcgac gccctcgtgt tgcaactgga ccagatgctt
     3841 gacacctgcc ggtcgtatga taaaaacgct attcgcgcct atgctgaaaa acactttgct
     3901 tataaagtgg taggaaaaca atttttagaa atttataatc aggtgatttg ttcctaaaga
     3961 tatttaaaac attcggagta aggctgtctt ctgcaatcat cagtttactg atagccgtgg
     4021 ttatttctca atttcttggc gccggcggca agggcgaaca gggtattatc ataaccacga
     4081 ttgcgctgat actgattttt gccaacatgg tcggtggcgc gtcactggtg tatcttaccc
     4141 cgaaacttcc actgaaaaag cttatccttc cttcttatgt ctggagtttt tttatcagcg
     4201 ccctttttgt tggtgtctta tttctgtttc cccttattga ggcaaaatat gtctggcatg
     4261 tagcgttgct cactttgcta aactcgttta cttcggtgaa tacctcggtt ctgcttggaa
     4321 aggaaaatat ccgcgcggtc aacatcatca acttttccca ggtggcgctc accatatccg
     4381 ccttactggt tttttttatc tcgggaatag ccaccaatat ttacggatat atttactctt
     4441 tgtattttgc ctatgggtgc accctcgtga tgagtgtgat ccttctgagc ccttaccgtc
     4501 ataaatcgcc cggtgatgag ccggtgtcct atacttatgc tatcaggatg ctgtttcggt
     4561 atggatttat gaatcagttg gcgcatatta cgcagttgtt gagtttccgc gtgagttact
     4621 attttctgga atcctttcag ggttatgatg ccgtgggtat ctattccaac ggcgtttcca
     4681 taaccgagtc ggtatggatg atcagtggca gtgtggcatt ggtgcaatat tcaaaaatat
     4741 cgaacaccgg cgataaaaag tttaatcagc agcttagtgc cgaactgctt cgcatcagcc
     4801 tgttgttgtc ttttttggtg ctgataccgc tggtgttgtt tccgggctct ttttatgcct
     4861 ttatttttgg taaagatttt ggcgatatca accgtatcat gtggctgttg gctccgggag
     4921 tgctggtatt tgtcaatgcc ttgattctgg ggcactattt ttccgggacg ggacaatatc
     4981 atattaacag tatcggttcg gctatagggc tggtggtcac cataatactt gcgctggtgc
     5041 tcattccccg catgggatat cttggcgcag cgcttacagc cagcctttcg tatctatcca
     5101 ccacggtgtt tgttactatc tgtttctgcc gcgaatcaaa gctgaagctg atagatctgt
     5161 tgccccttcc gagatatttt tcaggatatt atttgtcggt aaaacaatat ctttgcaaaa
     5221 acaaagctgc acgggatgag taaaatttca aaagctttca aagcgctggg gatgattgtc
     5281 cgcaggccat acctgctaaa ccttatcaat gatgatgagc aggtgaaaaa aaa
//
LOCUS       DAOSFP010000144         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13689_length_5331_cov_4.251516, whole genome shotgun sequence.
ACCESSION   DAOSFP010000144 DAOSFP010000000
VERSION     DAOSFP010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13689_length_5331_cov_4.251516"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PLI16_05415"
     CDS             complement(<1..1042)
                     /locus_tag="PLI16_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOH84033.1"
                     /translation="MWLNINGANRLLICNPEKDSLADVVRRMGLTGTKVGCDVGVCGS
                     CSVILNGKLVRSCTKKMKNIDEYSSVLTIEGIGTPLNLHPIQAAWIHAGAVQCGFCVP
                     GFIVSTYALLHENVNPTREEVRDWFQKNKNVCRCTGYVQIVDAVMQAAAVMRGEKTMD
                     DLLFKKPADGEYYGKPVHRPSALAKVCGLADYGDDQELKMPSEKLHVALVQPRIAHHA
                     RILKIDMDEALKMPGIVKIVTAQDIKDAGGTNIMAEGHFHERTTIAVPTRNVLCEDKI
                     NRWGDVVALVCADTKDRARAAAAKVKVEIEKLPEYLGYLDAAMPDAMRIHEDTPNIFA
                     MQPVLKGAGLEAP"
     gene            complement(1102..1542)
                     /locus_tag="PLI16_05420"
     CDS             complement(1102..1542)
                     /locus_tag="PLI16_05420"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOH84034.1"
                     /translation="MTKDRVAEALEMFTAGFHCSQSVFAVFSEDFGVPKETALKIACP
                     FGGGLGGYGRTCGAITGAMMVLGLRYGSSDPTDIETKRISREKTRALIESFEKQHYSC
                     NCNDLVGFDRSQLSDADLMLKLPVFHNTCGKFLETVVAYLEEEL"
     gene            complement(1564..3114)
                     /locus_tag="PLI16_05425"
     CDS             complement(1564..3114)
                     /locus_tag="PLI16_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH84035.1"
                     /translation="MENKIKQVLEKFGIKELNDGVCTGTEWFKTEGDVTVSVSPIDNR
                     EIAKVKNATLNDYEAVMQKAGEAFKIWREVPAPQRGEIVRQIGLALREAKEDLGFLVS
                     LEMGKIHQEGMGEVQEMIDICDFSVGQSRLLNGFTMHSERKDHRVYDQYHPLGIVGLI
                     TSFNFPVAVWAWNSMLAAVCGDVIVWKPSSKTPLCAIATQNIIGGVLKKNNIPEGVFN
                     MIIAKSSVLGDNFVADKRIPLFSITGSTRVGKRACKIIGERLGKSILELGGNNAVIVS
                     KYADLNMAIASTVFGAVGTCGQRCTSTRRIIVHKKVYNEVKYNLISAYEQIREKIGNP
                     LNAETLVGPLVDESAVNAFLNAIEEVKKEGGKVLYGGYKIEDENNISGNYVVPALVEA
                     ENHYKIVQEETFAPILYLIKYDGEIEEAIAINNDVPQGLSSSIFTLNMREAEKFLADA
                     GSDCGLANVNSGTSGAEIGGAFGGEKDTGSGRESGSDSWKQYMRRQTNTINYSNELPL
                     AQGIKFEF"
     gene            complement(3326..5056)
                     /locus_tag="PLI16_05430"
     CDS             complement(3326..5056)
                     /locus_tag="PLI16_05430"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOH84036.1"
                     /translation="MYKKKLSNKWLKSSVLGCLWASAEIVLGSFLHNLKIPFTGNILT
                     AIGIVLLISVAHIWKEKGLFWRSGIVCALMKSISPSAVIFGPMIAILTEALAMELVVR
                     IFRRNIFSFLLAGIFAMVSNLIFIIFNYVIIYGTSILDLYKNLTEFATRQLHLSGDIY
                     WMPLFLLICLFVFMGLIAAVFGIYIGRKAAAQPVKTESLSVKQVLDIKSKKHDTSFAF
                     SYYWLAFNMVALIAVLALMSFTPYYYWVSAGCIVLAVWFIRYKRALRMLAKPTFWITF
                     FLLTMISAFILYELKNSEKGFVDGILIGLEMNFRALVVIIGFMAIGTELRNPGFRKIF
                     VNSRFWQLPLALEVAFDTLPLMIANLPRVRDVFKKPAAIFRILVAQADFWLEKTELKL
                     NDRKNVIIISGSEKTGKSTLLQKIVECLEMKHLKAGGFLSTSVFENGLHTGYDIVDLK
                     DYSKKILSRIYGDETMAKVGTFYFRPDGIDFGNELLTLKNLSHADFVVIDEIGPWELK
                     NQGWAKGLSSLVRYWEKPIILVVRESIVDKVIENWSLKEPLIADSKNCDINFICNHVI
                     SILRLQKVGS"
     gene            complement(5139..>5331)
                     /locus_tag="PLI16_05435"
     CDS             complement(5139..>5331)
                     /locus_tag="PLI16_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOH84037.1"
                     /translation="EGLSEHTAAFDALVNIVDGTADIIINGKSNILHAGQFIIMPANI
                     PHAVKAVEKFKMVLVMIKS"
BASE COUNT         1501 a         1259 c          984 g         1587 t
ORIGIN      
        1 gcggggcttc cagcccggca cctttaagca ccggctgcat ggcaaaaata ttaggtgtat
       61 cttcatgaat acgcattgca tccggcatgg cagcatcgag gtatccaaga tactcaggaa
      121 gtttttcaat ttccacttta actttagcag ctgcagcccg ggcgcggtct ttggtatcgg
      181 cacaaactag tgccacaaca tcgccccagc gatttatttt atcttcacaa agcacattac
      241 gtgtaggtac agcaatggtt gtacgttcgt gaaaatgtcc ttcagccata atattggttc
      301 caccggcatc tttgatatcc tgcgctgtta ctattttcac aatgccgggc attttcaatg
      361 cttcgtccat atcaattttc aaaatcctgg catgatgagc tattcggggt tgaaccaaag
      421 ctacatgcaa tttttcggaa ggcattttca gttcctggtc atcaccataa tctgccagtc
      481 cgcaaacttt agccaaagct gacgggcggt gaaccggctt accataatat tcaccatcag
      541 cgggtttttt aaacaacagg tcatccatgg ttttttctcc acgcatcacg gctgccgcct
      601 gcattactgc atcgacaatc tgcacataac ctgtacaacg gcaaacattt ttattttttt
      661 gaaaccagtc gcgcacttct tcgcgtgtgg gattcacatt ttcgtggagc agggcgtatg
      721 tcgaaactat aaagcccggg acacaaaaac cgcactgtac tgcgccggca tgaatccacg
      781 ccgcctgaat gggatgaaga tttaatggag taccgatacc ttctatggtc agaaccgagc
      841 tgtactcatc gatgtttttc attttttttg tacaggagcg taccagtttt ccgtttaaaa
      901 ttactgaaca tgacccgcaa acaccaacat cgcatcccac tttggtacct gtcaggccca
      961 tccgccttac tacatcagca aggctatcct tttcaggatt acaaattaat aagcgatttg
     1021 ccccgttaat attgagccac atttttctaa ggttcataag atgttctcct tttaattatt
     1081 ggttgttaaa attgatattt attaaagttc ttcttcaaga tatgcaacaa cagtctccag
     1141 aaatttccca caagtattat ggaaaacagg cagctttagc atcaggtcgg catcgctgag
     1201 ctggctccgg tcaaaaccca caaggtcatt gcagttgcag gaatagtgct gcttttcaaa
     1261 gctttcgatg agtgcacggg ttttttcacg cgaaattctc tttgtttcaa tatcagtcgg
     1321 atcgcttgac ccatatctca gcccaagcac catcatggct ccggtaatgg cgccacaggt
     1381 tctgccatat ccgcccaggc ctccgccaaa agggcacgca atcttcaagg cagtttcttt
     1441 tggcaccccg aaatcttcgc tgaacacggc gaacacggat tgcgaacagt gaaaacctgc
     1501 tgtaaacatt tcgagcgctt ctgcaacacg atcttttgtc attccagttg tataagataa
     1561 ttgctaaaac tcaaatttga taccttgtgc caatggcagt tcgttactat aattaatggt
     1621 attggtttgt cttctcatgt attgtttcca ggagtcagac cctgattcac gcccgcttcc
     1681 ggtgtctttt tcgccgccaa aggcgccgcc tatctctgca cccgatgtac cgctgttgac
     1741 attggccagg ccacagtcgg agccggcatc ggcaaggaat ttttctgctt ccctcatgtt
     1801 gagggtaaaa atggaagacg acaaaccctg tggcacatcg ttattgattg ctatggcttc
     1861 ttctatttct ccatcgtatt ttataagata aaggatcggt gcaaaagttt cttcctgaac
     1921 tattttataa tgattttcgg cttctaccaa tgccggcacc acataattcc cggaaatatt
     1981 attttcatct tctatcttat agccgccata taacactttt ccgccttctt tttttacttc
     2041 ttctatagcg tttaagaaag catttaccgc actctcatca accagaggac ccaccaatgt
     2101 ttcagcgttc agcggatttc cgattttttc ccgaatctgt tcgtaagcac tgataagatt
     2161 atattttact tcattataaa cctttttatg tactattatt cttcttgtcg aagtacaacg
     2221 ctgcccgcag gtacccacag cgccaaacac cgtggaagca atggccatgt tcaggtcggc
     2281 atacttagaa acgattaccg cattgttgcc acctaattca aggatagatt ttccaaggcg
     2341 ctcaccaata attttgcatg ctcgttttcc cactcgggtg gaaccggtaa ttgaaaacag
     2401 cgggattctc ttatcggcga caaaattatc gccgagaacc gaagattttg caatgatcat
     2461 gttgaataca ccctcaggaa tattgttctt ttttaaaaca ccgccgataa tattttgagt
     2521 agctatagcg catagcggcg tttttgaact tggcttccat acaatcacat cgccgcaaac
     2581 ggctgccagc atagagttcc atgcccaaac agccacggga aagttgaatg acgtgattaa
     2641 gcccacgatt cccagcggat gatattggtc gtaaacacga tgatcttttc gttccgagtg
     2701 catcgtaaac ccgtttagta atcgtgattg ccccactgaa aaatcacaga tatcgatcat
     2761 ttcctgaact tcgcccatac cctcctggtg aatttttccc atctcaagag aaaccagaaa
     2821 acccagatct tctttggctt cgcgcaaggc caggcctatc tgtcgtacga tttcacctcg
     2881 ctggggagca ggtacctcgc gccatatttt aaaagcttct ccggctttct gcatgacagc
     2941 ctcgtaatca ttaagtgttg cattctttac ttttgctatt tcgcgattgt ctataggcga
     3001 tacagagacc gtaacatctc cctcggtttt aaaccattcg gtgccggtgc atacgccatc
     3061 gttcaattct ttaataccaa acttttctaa tacttgttta attttatttt ccatctttat
     3121 tgttcatttt tcatttattt ccttttgtca tagctcagtt tagaagacac catatataat
     3181 cacagtaccc ttcaccaaca aaactgaaaa tttctgatat tcgatatgaa tttgtttgca
     3241 tatccaaaac aaaagtaatt attattttgg ttctaaattt tatatttgaa aaatcgtggt
     3301 tagaatttta taatctgaaa acgtatcagc ttcctacttt ttgcaatctt aatatggaaa
     3361 ttacatgatt gcatataaaa tttatatcgc aattttttga atcagctatc agcggttctt
     3421 tgagcgacca gttttcaatg actttgtcca caatgctttc gcggactacc aaaattattg
     3481 gtttttccca atacctgacg aggcttgaca accccttcgc ccatccctgg tttttcaatt
     3541 cccatggccc tatttcatca ataacaacaa aatcggcatg gcttaaattt ttcaatgtta
     3601 acagctcatt gccaaagtca atgccatcag gtctgaaata aaaagttcct actttagcca
     3661 tggtttcatc gccataaatc cgggacaaaa tcttttttga ataatccttc aggtcaacaa
     3721 tgtcatatcc cgtgtgcagg ccgttttcaa ataccgacgt tgacaggaac ccacctgctt
     3781 taaggtgttt catctcaaga cattccacga ttttttgcaa caatgtggat tttcctgttt
     3841 tttccgaacc actgataatg attacatttt ttctgtcatt taatttcaac tctgtttttt
     3901 cgagccagaa atcggcctgg gcaaccagta tgcgaaatat cgccgcgggt tttttgaaaa
     3961 catccctgac acggggcagg ttggcaatca taaggggtag cgtatcaaag gcaacctcca
     4021 gcgccagcgg caactgccaa aacctcgaat tgacaaatat ttttctgaat ccggggtttc
     4081 tcaattctgt gcctatagcc ataaatccta tgataacaac caaagccctg aaattcattt
     4141 ctaatcctat caggatgccg tcaacaaatc ctttttcaga attttttaat tcgtataaaa
     4201 taaaggcaga aatcatagtc aacaggaaaa atgtaatcca gaatgtaggc ttggcaagca
     4261 ttctgagagc gcgtttgtag cgaataaacc acacggccaa aactatacat cctgctgaca
     4321 cccaataata ataaggtgta aaactcatca gtgccagcac agcaatcaat gccaccatat
     4381 taaaagcaag ccagtaatat gaaaaagcaa aggaagtgtc gtgttttttt gattttatat
     4441 ccagcacctg tttcaccgaa agactttcgg tttttacggg ttgtgcagca gcttttcggc
     4501 cgatataaat tccgaaaacg gcagcgataa gccccataaa cacaaaaaga caaataagca
     4561 ggaataaagg catccaataa atatcacccg ataaatgcaa ttgccttgtg gcaaattctg
     4621 tcaggttttt ataaaggtca aggatgctgg tgccataaat tataacataa ttaaatatga
     4681 taaaaatcag gttcgacacc atggcaaaaa ttcctgccaa taaaaaagaa aaaatatttc
     4741 tgcgaaaaat gcggactacc agctccatag caagggcttc ggtaagaatg gcgatcatag
     4801 gaccaaagat cactgcgctg ggcgatatgg atttcatgag ggcgcagaca atacccgacc
     4861 gccagaacaa tcctttttct ttccagatat gagcaacgga aatcaacaat acaatgccaa
     4921 tagccgtcaa aatatttccc gtgaaaggta tcttcagatt gtgtaaaaag cttcccagta
     4981 ctatttcggc agatgcccac aggcagccca aaacagacga tttaagccat ttattgctca
     5041 gttttttttt gtacattttt tcttaagaat acctgttcaa aaacggtatc atcaattttt
     5101 gacgtgaata aaaatacaag ttgtaaaaaa acaaactctc agcttttaat catcaccagc
     5161 accattttaa acttttccac agctttcacg gcatgcggaa tattggccgg catgatgatg
     5221 aattgccctg catgcaggat gttcgatttg ccgtttatga tgatatcagc ggtgccatcc
     5281 actatattca ccaacgcatc aaaagccgcc gtatgttcgc ttaatccttc g
//
LOCUS       DAOSFP010000145         5302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13802_length_5302_cov_4.351248, whole genome shotgun sequence.
ACCESSION   DAOSFP010000145 DAOSFP010000000
VERSION     DAOSFP010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13802_length_5302_cov_4.351248"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="PLI16_05440"
     CDS             complement(<1..1747)
                     /locus_tag="PLI16_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOH84038.1"
                     /translation="MKKIILPLLLSMSLTAFSQNQNENLPDENIQTAVAGKVSHTKTS
                     QQNQEIQAESMPQNFKSIVQEESEKYSQYNFQRNEQWDSLFYAQNPGIPKTLLPKQNK
                     TCTLNKVVYGWNPYWAGTDYLNYQWNLLSHMCHFSYEVNYSTGNANNTHNWATDAAVN
                     AALAHGVKVDLCVTLMNQANLTSFLANSTARQTLITNLINLIQSRGANGVNIDFEGMA
                     SSDSINFKTFMIALCNQMHTAIPGSQVSYCLPAVEWTANKFSARALVNYVDMFIIMGY
                     DYYWGGSSMAGPTDPLYNFNTGYNYTLAKTITYYQNQGVPLSKIILGLPYYGKQWATT
                     DNSVGSSTTATGTSPTYKVVRANADGYYSTKLWNNTSFSPYYTFQIGSQWYQCWIDDA
                     YSLGKRFDLVNQRGIGGIGIWALGYDNGYSNYWNKISDKFSTCATVPCSDTIYDMGGP
                     ERNYYNGEDFTYTIAPTGAYGVKLQFSAFDVEAGSGSVCDYDYLEIFDGPNTTAPSLG
                     KFCNTTGSPGLISSTGNSLTLKWHSDGGTINPGFRAVWQCTTDNVAPVTTTNVVGAWQ
                     TQDFTATFTDTDNTGG"
     gene            complement(1924..2898)
                     /locus_tag="PLI16_05445"
     CDS             complement(1924..2898)
                     /locus_tag="PLI16_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOH84039.1"
                     /translation="MEKTPAYLKLLNDKLEEITFVKEPKELYEPMAYALTMGGKRLRP
                     VMTLMACEMFRGDVSAALPAALALEVFHNFTLVHDDIMDVAPLRRGKETVYKKWNTNI
                     GILSGDAMFAKAFEIIARSGEKTLKPLVQLLSKVAIEVCEGQQYDMNFEQSPDVSIAD
                     YLEMIRLKTAVLIASSLKAGAIVAGASEQDTNEIYLFGENLGMAFQLKDDILDIFSDE
                     KVFGKKKGGDIVAGKKTFMFLKAIEMADEDTRATLVNYFTDTTIEPDTKVKAVTVIYE
                     KLCVKKCAEDMMDAYSSKALSHLKNIRVPSADKKNLEQLSKELLLRNF"
     gene            complement(2898..4100)
                     /locus_tag="PLI16_05450"
     CDS             complement(2898..4100)
                     /locus_tag="PLI16_05450"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOH84040.1"
                     /translation="MKVLVLNCGSSSIKYQLIKMNEEADLLAKGLLERVGMDNSELTH
                     VPKGKDKVVSIQPIHDHTDGINLILKALLHPEHGVIQDKHEIDVVGHRVVHGGEKFSG
                     SVLITQEIIDKMEECSDLAPLHNPANLKGIYAIQKLLPDIKQCGVFDTAFHQTMPEHT
                     YLYALPYELYDKYKVRRYGFHGTSHGFVSQKACKLLGLKLEETKIITCHLGNGASIAA
                     IKNGKSFDTSMGLTPVEGLIMGTRCGDLDLGALLFLMEKEKLDIKQANALINKKSGMA
                     GITGVSSDMRDIEDAANKGNKRAALALKMYNYRVKKYIGAYTAGMEGVDLIIFTGGIG
                     ENAVNTREGVMQGLEFLGVDFDYELNKVSRGKDIVLTKPGSKVKVMVITTNEELVIAN
                     EAIKTISQ"
     gene            complement(4139..5137)
                     /gene="pta"
                     /locus_tag="PLI16_05455"
     CDS             complement(4139..5137)
                     /gene="pta"
                     /locus_tag="PLI16_05455"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOH84041.1"
                     /translation="MNLLENICDKAKQLDKRIVLPEGISERTLKAADIILKEGYAQLI
                     LLGNLEEIQSLEHKFNLDLSAARKIDPKTSPDKERYVQKLMELRKSKGITYEDAVKLI
                     EQDIYYGPMMIYMGDADGEVSGAEHTTADTVRPALQIVKTAPGISVVSGAMVMVTKKE
                     NLGEKGVFIFADVAITPNPTAEELAQIAVCSGDTARQIAGIENPRIALLSFSTKGSAK
                     HELADKVKQATGLAKAMRPEMNFDGEMQADAALIESIGQKKSPGSPVAGLANVLVFPD
                     LQAGNIGYKLVERLGDAEAIGPILQGLAAPINDLSRGCSVMDIVNLVAITACQSGK"
BASE COUNT         1254 a         1163 c         1255 g         1630 t
ORIGIN      
        1 acccgcctgt gttgtcagta tcggtgaagg tggccgtaaa atcctgcgtt tgccaggcgc
       61 cgaccacgtt tgttgtggtt acgggggcaa cattatccgt tgtgcactgc caaacagcgc
      121 ggaacccggg atttatggtg ccgccatcgg aatgccattt taaggtcagc gagtttcctg
      181 tggaggatat cagtcccgga cttcctgtgg tgttgcaaaa ttttccaagg cttggtgctg
      241 tggtgttggg cccgtcgaag atttccaggt agtcgtaatc gcacaccgag ccgctgcctg
      301 cttccacgtc aaaagccgaa aactgcagtt ttacgccgta agcgcctgtt ggtgcaatgg
      361 tataggtaaa atcttcgccg ttgtaatagt ttcgttccgg tccacccata tcataaatgg
      421 tgtcggaaca ggggacggtg gcgcaggtgg aaaatttgtc gctgatttta ttccagtaat
      481 ttgaatagcc gttgtcataa cccaaagccc aaattccaat accgccgata ccgcgctggt
      541 tgaccaggtc gaaacgtttg ccaaggctgt aagcatcgtc aatccagcac tggtaccact
      601 ggctgccgat ctgaaaggtg taataaggac tgaacgaagt gttgttccaa agtttggtgg
      661 aataatagcc gtcggcgttg gccctgacga ctttatatgt gggagaagta cctgtggctg
      721 tagtggaaga cccaacagaa ttatcagttg tagcccattg tttgccataa tatggcaggc
      781 ctaaaatgat ttttgataaa ggaacccctt ggttttgata ataagtaatg gtttttgcca
      841 acgtgtaatt atatcctgta ttaaagttgt acagaggatc ggtagggccg gccatagagc
      901 taccgcccca ataataatca taacccatga tgataaacat atccacgtaa tttaccagtg
      961 cccgggccga aaatttattg gcagtccatt ccacggcagg caggcaatag ctcacctggg
     1021 agccgggtat agccgtgtgc atctggttgc agagggcaat cataaatgtt ttaaaattaa
     1081 tagagtccga agaagccatc ccctcaaagt caatattcac gccatttgca ccccgcgact
     1141 gaataaggtt tatcaggttg gtgataagag tttgccgggc cgtgctgttt gccagaaaag
     1201 aggtcaggtt ggcctggttc atcagcgtta cacaaagatc aactttcacc ccgtgggcaa
     1261 gggcggcatt aacggcggca tctgtggccc aattgtgggt attgtttgcg ttacctgtgg
     1321 aataattaac ctcatacgaa aagtgacaca tgtgcgacaa caggttccat tggtaattca
     1381 gatagtccgt gccggcccag tatggattcc atccataaac aactttgttt agggtgcagg
     1441 ttttgttttg ttttggcagc aaagtttttg gtattccggg gttctgggca taaaaaaggc
     1501 tgtcccattg ttcattgcgt tgaaaattgt actgactgta tttttcgctt tcttcctgta
     1561 ctatcgattt gaaattttgc ggcattgatt ccgcctgaat ttcctgattt tgctgtgatg
     1621 tttttgtatg cgatactttc cctgctacgg cagtctgaat attttcatcc gggaggttct
     1681 cgttttgatt ctggctaaag gcagtaaggc tcatggataa cagcagcggg agaataattt
     1741 ttttcatgtc aggggaacag gttatgataa aacaaaaata aaaaaatata caaggcaaat
     1801 ggtatcgttt tccggtaaat ttttatcacg tggatgcttt cttatgcgca ggccttatat
     1861 ggttatgctg aaaactgccc ggaacaaaat ccggttgaaa taaatggcag aaattttacg
     1921 aaattaaaaa ttcctcaaca acagctcttt cgaaagttgt tccaggtttt ttttatcggc
     1981 tgagggcact cttatgtttt tgagatggct cagtgctttt gaggagtatg catccatcat
     2041 atcttcggca cattttttta cgcaaagctt ttcatagatg actgttacgg ccttcacctt
     2101 ggtgtcgggt tctattgtgg tatcggtaaa ataattgacc agcgttgcac gggtgtcttc
     2161 atcagccatt tcgatggctt tcaggaacat gaatgttttt ttcccggcca caatatcgcc
     2221 accctttttc tttccgaata ctttttcgtc actgaatata tccagaatat catctttcag
     2281 ttgaaaggcc atcccgaggt tttcgccgaa gaggtaaatt tcgttggtat cttgttcgga
     2341 ggcgccggcc acaatggccc ctgctttcag gcttgaggcg ataagcaccg cggttttcag
     2401 gcgtatcatc tccagataat cagcaatact aacatccggg ctctgttcga aattcatgtc
     2461 gtattgttgc ccttcacaaa cctcgatagc aacttttgaa agcagttgta ccaatggttt
     2521 aagcgttttt tcgccggagc gggcaataat ttcgaaggcc tttgcaaaca tggcgtctcc
     2581 tgacaatatt ccgatattgg tattccattt tttatatacg gtttcttttc ccctgcgcag
     2641 gggggcaaca tccataatgt cgtcgtggac cagcgtaaaa ttatgaaata cttccagggc
     2701 gagcgctgcc ggcaaggccg cagacacatc gccccggaac atttcgcagg ccatgagtgt
     2761 catcacggga cggagacgtt ttccgcccat ggtcaacgcg taggccatag gttcgtataa
     2821 ctctttgggc tctttgacaa aagtaatctc ttccaacttg tcattaagaa gcttgagata
     2881 tgccggtgtt ttttccatta ctggctgatc gtttttatgg cttcgttggc aattaccagt
     2941 tcttcgttgg tggtgataac cataacttta actttagaac ccggtttggt cagcacaatg
     3001 tctttgccgc gcgaaacttt gttgagttcg taatcgaaat ccacgccaag aaactcgagg
     3061 ccctgcatca cgccttcgcg ggtgttgaca gcattttcgc ctatgcctcc cgtaaaaatt
     3121 atcaggtcca cgccttccat tcctgcggta tacgccccga tatatttttt tacccggtag
     3181 ttatacatct tcagggccag cgctgcgcgt ttgttccctt tattggcggc atcttcaatg
     3241 tcgcgcatgt cagaagaaac accggtaatt ccagccatgc cgcttttttt gttgatgagg
     3301 gcgttggctt gtttgatgtc gagtttttct ttttccatca gaaaaagcag tgcgcccagg
     3361 tcgaggtcgc cgcagcgggt tcccataatg aggccttcca ccggggttaa tcccatggag
     3421 gtgtcaaagg attttccgtt ttttatggca gctatcgaag ctccgttgcc gaggtggcag
     3481 gtgatgattt ttgtttcttc aagttttagc cccagtaatt tgcaggcttt ttgtgaaaca
     3541 aaaccatggc tggtaccgtg gaatccgtaa cggcgcactt tgtatttgtc gtacagttca
     3601 taaggcaacg catataaata ggtgtgttcg ggcatggtct gatgaaacgc cgtgtcgaaa
     3661 acgccgcatt gttttatatc cggcaaaagt ttctgaatgg cataaatacc tttcaggttg
     3721 gctggattgt gcaaaggggc caggtcggaa cattcttcca ttttatcgat aatttcctgc
     3781 gtgataagta cactgccact gaatttttcg ccgccatgca ccacccggtg tcctaccaca
     3841 tcaatttcgt gtttgtcttg gataacgccg tgttcggggt gtagcaaagc cttgagaatg
     3901 aggtttatac catcggtatg gtcgtgtatg ggctgaatac ttacgacttt atctttgcct
     3961 ttgggcacat gggtaagttc gctgttgtcc atgccgacac gttccagcaa tcctttggcc
     4021 agcaggtcgg cttcttcgtt catctttatg agttggtatt taattgatga acttccgcag
     4081 tttaatacaa gtactttcat tttattttgt tattaagttt atatgtttgt tttgtaaatt
     4141 attttccaga ctggcaagcg gtaatggcca ctaaattaac aatatccatt accgaacatc
     4201 ctctcgacaa atcgttaatc ggggcggcta atccctgtaa aatagggcca atggcttcgg
     4261 catcgcccag ccgttccacc aatttataac cgatatttcc ggcctgcagg tcggggaata
     4321 ccagcacatt ggctaggccg gcgaccggtg agccgggaga ttttttttgt cctatagatt
     4381 ctatcagtgc ggcatcggcc tgcatttcgc catcgaagtt catttcgggg cgcatggctt
     4441 ttgctaaacc ggtggcttgt tttaccttgt ctgccagttc gtgtttggct gatcctttgg
     4501 tgctgaatga taataaggcg atacgcggat tttcaatacc tgcaatctgg cgggctgtgt
     4561 cgccgctgca aacggctatc tgggcaagtt cttctgccgt agggttaggc gtaatggcca
     4621 catcggcaaa aatgaagaca cctttttctc ccagattctc ttttttggtt accataacca
     4681 tagcgcccga aaccaccgat atgccgggag cggtttttac tatctgcagg gcaggcctta
     4741 cggtgtcggc cgtggtgtgc tcagcaccgc tcacttcgcc gtcggcatcg cccatatata
     4801 tcatcatcgg accgtagtat atatcctgtt caatgagttt tacggcgtct tcgtaagtga
     4861 tgcctttgct tttacgcaat tccatcagtt tttgtacgta acgttccttg tcaggagaag
     4921 ttttcgggtc aatcttgcgt gctgccgaaa ggtcaagatt gaatttatgt tccagcgatt
     4981 gtatttcttc taaatttccc aggagaatta gttgtgcata tccttctttt aatataatgt
     5041 cggctgcttt cagggtgcgt tccgaaatgc cttcgggcag gacgatgcgt ttgtcgagtt
     5101 gtttggcttt gtcgcagatg ttttctagta aattcataaa atactgtgta gttgagtaaa
     5161 taaatatttg aatgattaaa ttttttgaca aaagaaaaaa aattattgat accgagccaa
     5221 tttattttga tatttccgac aacatattat caatttctgt tttgattcaa aattctatct
     5281 gattttaatg gatatgaatt ag
//
LOCUS       DAOSFP010000146         5255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_13967_length_5255_cov_4.517500, whole genome shotgun sequence.
ACCESSION   DAOSFP010000146 DAOSFP010000000
VERSION     DAOSFP010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13967_length_5255_cov_4.517500"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1606
                     /locus_tag="PLI16_05460"
     CDS             <1..1606
                     /locus_tag="PLI16_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH84042.1"
                     /translation="PIGATGAQGPTGANGAQGLQGIQGPVGATGAKGATGATGAIGAQ
                     GIQGPIGATGAQGPTGANGAQGIQGIQGPVGATGAKGATGATGAIGAQGIQGPIGATG
                     AQGPTGANGATGPLVAGTFGQTLRHDGTTWVANSIIYNNGTNVGIGYTSPANRLVING
                     AATSGKLNKIVYVDGVTYTTIDQAISALPVSGGKVVIPEGTWTISSAISISGKSNIVI
                     EGAGPGTIITSAAGSNFNLFYVYQSSYITIRDLKVVSPDRTQYNNHGVYIYESKNCVV
                     ENCLFTDCNNGVYLYHADNTSNPTDNNLITNNNFFQCATGIRLYSSKYAAETDVATSQ
                     VKNNVITNNFIDCNDIASSNGIYSEWYGYNNIISGNIINKPAGYGIKLTYTYSATIEG
                     NTISLPTSYGIHAQGGHYSKINGNNVINCLSTTVEGIKALLGTHMVISNNIVTSTLYT
                     TPTGIDVGGSYITITGNNVQNFNRGYWISATYSTIMSNAHRMISSTSGTNGFYVTGSY
                     NSVVGNRSNKANSYTGTNNYVAGNYP"
     gene            complement(1690..3330)
                     /gene="pckA"
                     /locus_tag="PLI16_05465"
     CDS             complement(1690..3330)
                     /gene="pckA"
                     /locus_tag="PLI16_05465"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOH84043.1"
                     /translation="MSCDCSKNAKPDLSIYGIENVQEVFYNLSYEELFIHETDEKLVG
                     YDKGFVTNMGAVNVDTGIFTGRSPKDKYIVRESENEGNVWWASPERKGSDNKPISEET
                     WEHMKTIARKQLSGKKLYVSDAFCGANVNTRIKIRVITEVAWASHFVKNMFIRPTEEE
                     LKDFTPDFVVYHACKATNPQWKEQGLNSEVFVSFNLKERMAVIGGTWYGGEIKKGFFS
                     VMNYFLPLKGIASMHCSANMGKDGDTAIFFGLSGTGKTTLSADPKRFLIGDDEHGWDE
                     EGIFNFEGGCYAKCINLSKENEPDIYNAIKRDALLENLVYDEKTGAIDFKSNAKTENT
                     RVSYPIYFINNIVKPVSKGPHPKNIIFLTADAFGVLPPVAKLNYDQTMYHFLSGYTAK
                     VAGTERGIKEPTPTFSSCFGAAFLLLHPTKYAQELAKKMQEHGSSAYLVNTGWIEGPY
                     GVGRRIDIPSTRAIIDAILDGSLESAEYEELPVFGLMIPKAVNGVNSKILNPRNIWSN
                     PADWDVAAKNLGEKFIKNFTSFTDNDEGKRLVGAGPKI"
     gene            complement(3539..4513)
                     /gene="rfaD"
                     /locus_tag="PLI16_05470"
     CDS             complement(3539..4513)
                     /gene="rfaD"
                     /locus_tag="PLI16_05470"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOH84044.1"
                     /translation="MQKYIVVTGAAGFIGSCMVSKLNNKGIKDIVIVDDFQKGYKLDN
                     INGKAYAKHIQREVFFDWFRENHKEVEFVFHLGARTDTAEFDKSIFDTLNLNYSKTMW
                     SICTEYEVPLLYASSAATYGMGEYGYFDDESLIPLLKPLNPYGESKNEFDKWVITQKN
                     TPPFWVGLKFFNVYGPNEYHKNRMASVVFHAYNQINETGTMQLFRSHNPNFADGKQMR
                     DFIYVKDVVRVCMFFYLTQKKSGIYNLGTGEARTFWDLVTNTFKSMRLTPRVEFIDTP
                     VDIRDKYQYFTEAKINKLIASGYDLGFTSLEEGIEDYVQSYLLHKQYY"
     gene            complement(4637..>5255)
                     /locus_tag="PLI16_05475"
     CDS             complement(4637..>5255)
                     /locus_tag="PLI16_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOH84045.1"
                     /translation="YRKKAELIATQSYPIIKDVYENNPKRYENIAIPITDGSKTLQVV
                     ANLRRSYENKGKDVILAIEKGISLALIDDAWKEHLREMDDLKQSVQNATYEQKDPLLI
                     YKKESHQLFDTMWQKISKDIVSFLLKGDLPFRNSENIQEARQSKTDMSNLKAGRDEGM
                     EQARNQRTSEVQRTEPVKAEKKVGRNDPCPCGSGKKYKNCHGLGQ"
BASE COUNT         1509 a         1129 c         1051 g         1566 t
ORIGIN      
        1 ccccatagga gccacaggag cacaaggacc aacaggagct aacggagccc agggattaca
       61 aggcattcag ggacctgttg gtgcgacagg cgctaaaggc gcaacaggag ctaccggcgc
      121 aattggagca caaggtatcc agggccccat aggagccaca ggagcacaag gaccaacagg
      181 agctaacgga gcccagggaa tacaaggcat tcagggacct gttggtgcga caggcgctaa
      241 aggcgctaca ggagctaccg gggcaattgg agcacaaggt atccaggggc ccataggagc
      301 cacaggagca caaggaccga caggagccaa cggagccacc ggcccattag ttgcgggaac
      361 attcggacaa accctacgcc atgatggaac gacatgggta gctaacagta tcatctataa
      421 taatggtact aacgtcggca ttggctacac ctctccggca aaccggctgg ttataaacgg
      481 tgccgccacc agcggtaagt tgaataaaat agtttatgtg gatggggtta cgtacaccac
      541 tattgatcaa gccataagcg cattacctgt ttctggcggc aaggtggtaa ttcctgaagg
      601 aacatggaca atttcttctg ccatatccat ttcggggaag tcgaacatcg ttattgaagg
      661 cgccggcccg ggcaccatta ttacttccgc agcaggcagc aacttcaatt tgttttatgt
      721 ttatcagtca tcgtatatta ctattcgcga cctgaaagtc gtatcccctg atagaacgca
      781 atacaacaat cacggggttt atatttacga atcaaaaaat tgtgttgtcg agaattgctt
      841 gtttacagat tgtaacaatg gagtgtatct ttatcatgct gataatactt ccaatcccac
      901 tgataacaac ctgattacaa acaacaattt ctttcagtgc gcaacaggta tcagattata
      961 ttccagcaaa tatgccgctg aaactgacgt ggctacaagc caggtaaaaa acaatgtcat
     1021 aacaaataat tttattgatt gtaatgatat tgccagcagc aacggtattt attccgaatg
     1081 gtacggatat aacaacatta tttctggaaa tattattaat aaaccagcag ggtatggtat
     1141 caaactaacc tatacctatt cagccaccat tgaaggaaat actatttcat tacctacatc
     1201 ttacggtatt cacgcccagg gaggccacta tagcaaaata aacggaaaca atgttattaa
     1261 ttgcctttca acaacagttg aaggaattaa agctcttcta ggcactcaca tggtcatatc
     1321 aaacaatata gttacttcaa cattatatac cacacctact ggcatagatg taggtggcag
     1381 ttatataacc attacaggca acaatgttca gaattttaac cgtggatatt ggatttctgc
     1441 tacctattcg acgataatga gcaatgctca ccgcatgata agttctacaa gcggcactaa
     1501 tggcttttat gtgacaggaa gttataactc ggtggttgga aaccggtcaa ataaagcaaa
     1561 cagttatacc ggtacaaata attatgtagc aggaaattat ccataataaa ggtattttat
     1621 tggtaccagg ttaaaagaaa aaagccgttg aaaactttca gcggcttttt ttatgattct
     1681 tacttgtaat taaattttag gtccagcacc taccaggcgt ttgccttcgt cgttatcggt
     1741 aaaactggta aagtttttaa taaacttttc tccaaggttt ttcgcagcaa catcccagtc
     1801 ggcgggattg gaccagatat ttcttggatt caaaatcttt gaattaacgc cgttaaccgc
     1861 tttgggtatc atcaacccaa agaccggcaa ttcttcatat tcagcacttt cgagcgagcc
     1921 gtccaaaata gcatcgatga tggcacgtgt tgaaggtata tcaattctgc ggccaacgcc
     1981 ataaggacct tctatccaac cggtgtttac caggtaggct gaagaaccgt gttcctgcat
     2041 ttttttggca agttcctgtg catattttgt agggtgtaac aaaagaaatg cggcgccaaa
     2101 acacgaagaa aaggtcggtg tgggttcttt tataccgcgc tctgtaccgg ctaccttagc
     2161 agtataacca ctcaaaaaat ggtacatcgt ctggtcataa ttaagtttgg caacaggagg
     2221 taacacgcca aaagcatcgg cggtaagaaa aataatattc tttggatgag ggccttttga
     2281 aaccggcttt acaatgttat tgataaaata aatgggataa gaaacgcgcg tattttcggt
     2341 tttggcattg ctcttaaaat caattgctcc cgttttttca tcgtaaacaa ggttttccaa
     2401 cagggcatct cttttaatgg cattgtatat atccggctca ttttctttgc tgaggtttat
     2461 gcattttgca taacaaccac cctcgaagtt gaaaatgcct tcttcgtccc agccatgctc
     2521 atcatcaccg atcagaaaac gcttaggatc ggcagagagt gtagtttttc cggttcctga
     2581 caggccaaag aaaatagccg tatcgccatc ttttcccata ttggcagaac aatgcatgga
     2641 agcaatacct ttcaatggca gaaaataatt cattactgaa aagaatcctt tcttgatctc
     2701 cccaccatac catgtccctc caatcactgc catacgttct ttcaggttaa atgaaacgaa
     2761 cacttcactg tttaatccct gttctttcca ttgcgggttt gttgccttac atgcatgata
     2821 cacaacaaaa tccggggtga aatcttttaa ttcttcttct gttggcctga tgaacatgtt
     2881 ctttacaaaa tgtgatgccc aggcaacttc cgtaattacc cggattttta tccgcgtatt
     2941 gacgttggcg ccgcaaaaag catcgctgac ataaagtttt ttcccgctga gttgtttcct
     3001 ggcgatagtt ttcatgtgct cccaggtctc ttctgatatg ggtttattat cagaaccttt
     3061 acgttcagga cttgcccacc agacattacc ttcattttcg gattctcgga ctatatactt
     3121 gtccttagga gaacgaccgg taaagatgcc ggtatccaca tttacagcac ccatattggt
     3181 tacaaaacct ttgtcataac ccactaattt ttcatccgtt tcgtgaataa acagttcttc
     3241 gtatgacagg ttataaaaaa cttcctgaac attttcgatt ccataaatgg aaagatcagg
     3301 tttggcattt tttgaacaat cgcaagacat aattaatatt ttttaagtta ttagattgga
     3361 ttactgatat tttttgcaaa cttagtaatt taaataatgt taataatcac aaatccttaa
     3421 acaaaaaaaa atgattttgt ttgatttccc ataacccaca attcacatag gatgcagaaa
     3481 aaatgctgca atcagctatg atttatttta gattaaatct aattaaactt tcagggtatt
     3541 aataatactg tttatgcagc aaatacgact gtacataatc ttcgataccc tcttcgagcg
     3601 aagtaaatcc aaggtcatag ccggatgcta tcaacttgtt gatttttgct tcagtaaaat
     3661 actggtattt atcacggata tcaacaggag tatcgataaa ttccacgcgg ggagtcagcc
     3721 gcattgattt aaaggtattg gtaaccaggt cccaaaaagt acgggcctca ccggtgccaa
     3781 gattgtaaat gcctgatttt ttctgcgtca ggtagaaaaa catacatacc cttacgacat
     3841 cttttacgta gataaaatcc ctcatctgtt ttccgtctgc aaaattggga ttatgtgaac
     3901 gaaagagctg catggtgccg gtttcattta tctggttgta cgcatgaaac accacggagg
     3961 ccattcgatt cttatggtat tcgttaggcc cataaacatt gaaaaacttc aggccaaccc
     4021 agaaaggcgg agtgtttttt tgtgtaatca cccatttgtc gaattcattt tttgattcac
     4081 cgtaagggtt gagaggcttc agcaggggaa taagactttc atcatcaaaa tagccatatt
     4141 cgcccattcc ataggtagct gctgaagagg catagagcag tggcacctca tattccgtac
     4201 atatcgacca catggttttg ctgtagttca ggttaagggt atcgaaaatt gatttatcaa
     4261 attcggcagt atctgtacgg gcgccaagat gaaatacaaa ttcaacttcc ttgtgatttt
     4321 cgcgaaacca gtcaaaaaac acctcccgct ggatatgctt tgcataagct ttcccgttga
     4381 tattgtcaag tttataacct ttttgaaaat catccacaat aacaatgtct tttatacctt
     4441 tgttattgag tttcgagacc atacaactgc caatgaaccc ggcagcacct gttaccacaa
     4501 tatatttttg catcggattt ataataaatt atttctgggt taaatgatga ggtttataat
     4561 aatctgccaa acaatcggta ttttactatt cccaacttat tatctgcacg aatttggttg
     4621 tcctcccaat tcctacctat tgtcctaacc cgtgacagtt tttatatttt tttccgcttc
     4681 cgcaagggca gggatcattt cttcctactt ttttctcagc tttcacgggt tctgttcttt
     4741 gaacctctga agtcctttga tttctggcct gttccatacc ttcgtcgcgg ccggctttca
     4801 gattactcat atcggttttg ctctgccgtg cctcctggat attttcagaa tttctgaatg
     4861 gcaaatcgcc ttttagcagg aaagaaacga tatccttgct gatcttctgc cacatggtat
     4921 caaacagttg gtgcgattct tttttataga tcagtaaggg gtctttctgc tcgtaggtag
     4981 cattctgaac cgactgtttc aggtcgtcca tttcgcgcag gtgttctttc caggcatcat
     5041 cgataagtgc taacgaaatt cccttttcaa tggccaggat gacatctttt cctttgtttt
     5101 catatgaacg gcgcaggttg gccaccacct gaagcgtttt gcttccgtcg gtgatgggaa
     5161 tagcaatgtt ttcgtaccgt ttgggattgt tttcgtaaac atccttaatt atcggatagg
     5221 actgtgtggc gattaactcc gcttttttgc ggtag
//
LOCUS       DAOSFP010000147         5192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14206_length_5192_cov_3.456492, whole genome shotgun sequence.
ACCESSION   DAOSFP010000147 DAOSFP010000000
VERSION     DAOSFP010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14206_length_5192_cov_3.456492"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..528)
                     /locus_tag="PLI16_05480"
     CDS             complement(181..528)
                     /locus_tag="PLI16_05480"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOH84046.1"
                     /translation="MRNFFKVSVLILVVLLCTGGVHAQKVFSCDYKSDAKVKVFVADY
                     KSDADLVVYKCSYKSDATGNQGLWYFCDYKSDADVTIFFCDYKSDADLVIYFADYKSD
                     AGWKNSSKKHLLY"
     gene            complement(544..798)
                     /gene="purS"
                     /locus_tag="PLI16_05485"
     CDS             complement(544..798)
                     /gene="purS"
                     /locus_tag="PLI16_05485"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF004630.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOH84047.1"
                     /translation="MKFLAEINIMPLKELLDPQGKAVISGLKNMGITEITDVRIGKHI
                     SVEIEAKDEKTARVKLEKACKELLSNPIMESFSYEIKTQK"
     gene            complement(804..1547)
                     /gene="pssA"
                     /locus_tag="PLI16_05490"
     CDS             complement(804..1547)
                     /gene="pssA"
                     /locus_tag="PLI16_05490"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOH84048.1"
                     /translation="MKALKTNIANIVTSMNLLCGSLAIYFLFRDEFMLAVYLILAAAV
                     FDFADGFVARLLKVKSELGKQLDSLSDVISFGLAPGVAMFLMLERGLDFWPTGLPGQV
                     AFCAFLIPIFSAWRLAKFNIDERQTDRFIGLPTPANALLIFGLVPLSLGLGFLSWLPL
                     PEGIQYYIIHPALLIPISLVFSYLLVSPLPLIAMKFKTFAWKENRIRYIFILLSLIAV
                     LLFFFAAVPIIIILYIILSIIDLFIKRKK"
     gene            1628..3031
                     /locus_tag="PLI16_05495"
     CDS             1628..3031
                     /locus_tag="PLI16_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84049.1"
                     /translation="MKNFLIYGMVAMCTLMFSCSGNKKNNRAEEQALEKGKIISKVKC
                     LKNRTLNYAAYIPSTYSEDKKYPVIFCFDPSGNGVTPVEILMDDAEKLGYILVGSNVS
                     KNGLAWDIIADHYDVLLADVTERLSVDSARIYTCGFSGGSRVASSIAILKGGISSVIG
                     CSAGLPQLKEAIKTKFDYIGFAGSDDMNYAEMVNLDGALEKNNIRHQLVVFEGTHAWP
                     PKEVLSEAFTWMELHAMRDKKKPEDKDYINNKLSEYTGQLDEVKKSGNKYKEYLLTKK
                     MVTYFKGLADIAALEESLKKLQDNAAVKSAIDNAILNLKKELAMQQKYGPKIRTESAA
                     WWKNEVAYLNKFIAMSQNEDEKHIVKRVLEYLSLSAFSASSSLYNQNKFADAEHYIEI
                     YSIIDSDNPEPAFMKAQLAARKKDVDQTLEQLKKAASLGFAERSRVDNDTIFMKFKEK
                     KGFSNIMDLISENKNKK"
     gene            3084..3824
                     /locus_tag="PLI16_05500"
     CDS             3084..3824
                     /locus_tag="PLI16_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOH84050.1"
                     /translation="MPKIVFEVCANSLSSALAARDGGAHRIELCSSLNSGGLTPSAGT
                     IKSVLSLVDIPVFVLIRPRAGDFLYSRNEFNVMKEDIKFAKNLGAAGVVFGILNPEAG
                     IDVDRCSELVSLAKPMQSTFHRAFDRVMDPNSALEDVIKTGAQRVLTSGLNEKAVKGK
                     EFIRKLVEQANGRIAIMAGRGINPQNVAEIVSDTGVKEIHASCSASVYSKMIWLIRFS
                     SFDLDYCHTVTNACAVRKMLDVINKVDC"
     gene            3827..>5192
                     /locus_tag="PLI16_05505"
     CDS             3827..>5192
                     /locus_tag="PLI16_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOH84051.1"
                     /translation="MGKKLLLSIAALFCVLMVMGQDRSRHFINDTAYRGQVEKDFVKQ
                     QLIARGRAAQLFKVFDNNLSLEEEEALKFLYAYMPLSDLADYDGNFFLGQVRMSLLAK
                     KTFSWGDKISDDIFRHFVLPYRVNNENLDSARTVIFGELKDRIKNLSMHDAALEVNHW
                     CHEKVSYKPTDGRTSAPLSTIRTAYGRCGEESTLAVTAFRAVGIPARQVYTPRWAHCD
                     DNHAWVEVWVDGKWHFLGACEPEPDLDIAWFAAPVLRAMLCNTTVFGKYRAAEESLKS
                     DDQFTQINLIENYAPAKKLFVKTVDTNSRAVAGAVVEFQLYNYAEFYPLAKKNTDDQG
                     LAFLTTGLGDLLIWAYKGDTFGYIQVSVGKTDTAVVVLNKSAIENDEVIFTLVPPIER
                     QPKVAEAKGKKENEKRLQNEDKIRGAYEAGFIDSVSSCRFAQNLQLTTDTVWMFLKQS
                     RGNWH"
BASE COUNT         1561 a         1071 c         1169 g         1391 t
ORIGIN      
        1 catcaataag caaccagtct tttacaatga tttcgttgtt ggcagaacgt gttttataac
       61 taatagtatc catcatttat ttgaattaca gtaactaaac cttgtttctc tcaaaattgg
      121 ggtgcaaatg tactaatttt ttttcaatta gcaaatttac taacattttt atgttaatat
      181 ttagtacaac aggtgttttt tgctgctgtt tttccagccc gcatcacttt tataatcagc
      241 aaaatagatt accaggtcgg catcgctttt ataatcgcaa aaaaatatgg taacatcggc
      301 atcactctta taatcacaaa aataccataa cccctggttg cctgtggcgt cacttttata
      361 agagcatttg taaaccacca ggtcggcatc gcttttataa tctgcaacaa aaacttttac
      421 tttggcgtcg cttttataat cacaggaaaa tactttttgg gcatgaacgc caccggtgca
      481 caatagcacc actaaaatca gtacactgac tttgaaaaaa tttctcatgg gtatatttta
      541 ttgttatttc tgagttttta tttcgtatga aaagctttcc attatagggt tggataacaa
      601 ttccttacag gccttttcga gttttaccct ggcggttttt tcgtctttgg cttcaatttc
      661 caccgaaatg tgttttccta ttcgcacatc cgtaatttcg gtaatgccca tattttttag
      721 tccggatatg acggctttgc cttgcggatc gagcaattcc tttaatggca tgatattaat
      781 ttctgctaag aatttcatgg tgtttatttt tttcttttta taaataaatc aattatcgat
      841 aaaataatat acaaaattat aataataggc acagcagcaa agaaaaagag taaaacagct
      901 atcagcgaca acaggatgaa aatataacgg atgcggtttt ctttccaggc gaatgtttta
      961 aatttcatgg caataagcgg aagcggcgat accaggaggt aagaaaaaac cagcgaaata
     1021 ggaatgagta aggccgggtg gataatgtaa tactgtatgc cttcgggcaa cggcagccat
     1081 gagagaaatc ccaggcccag gctcaggggt accaggccga aaatcagcag tgcattggcc
     1141 ggggtgggta acccgataaa acgatctgtc tggcgttcgt ctatattgaa ttttgccagc
     1201 cgccaggccg agaatatggg aataagaaag gcgcaaaaag ccacctggcc gggcaaaccc
     1261 gtcggccaga aatcaagccc ccgttcaagc atcagaaaca tggctacgcc cggagccagc
     1321 ccaaaagaga taacatcaga gagcgagtcg agctgtttgc ccaattccga ttttaccttg
     1381 agcaaacggg caacaaaacc atctgcaaaa tcaaaaacgg ctgcggccag aataagatat
     1441 acagccagca tgaactcatc cctgaagaga aaataaattg ccagactacc gcatagaagg
     1501 ttcatgctcg taacgatgtt ggcaatattt gttttgagtg ctttcataag attttacaaa
     1561 aataatattt cgcttaacaa caactcataa acttttggtt tatttttgtt ttataataat
     1621 ttcagctatg aaaaatttct tgatttatgg aatggttgca atgtgcaccc tgatgttttc
     1681 gtgttcggga aacaaaaaaa acaaccgggc agaggaacag gcgctcgaaa aaggtaaaat
     1741 aatctccaag gtaaaatgcc tgaaaaaccg gacgctgaat tatgcggcct atatcccttc
     1801 cacttattcg gaggacaaaa aatacccggt tatattttgt ttcgatccgt cgggcaatgg
     1861 ggttacaccg gtggaaatac tcatggatga tgccgaaaaa ctcggatata tcctggttgg
     1921 atcgaatgtg tcgaaaaacg gcctggcctg ggatattatt gctgaccatt atgatgtatt
     1981 gctcgccgat gttacggaac gtttgtcggt ggattccgca aggatttaca cctgtggctt
     2041 ttcgggaggt tcgcgtgtgg ccagttccat tgccatcctc aaaggaggta tttcaagcgt
     2101 gattggctgt tcggcagggt tgccgcagct aaaagaagct ataaaaacca aatttgatta
     2161 tatagggttt gcaggcagcg acgatatgaa ttatgccgaa atggtcaacc ttgatggtgc
     2221 actcgaaaaa aacaatatcc gtcaccaact ggttgttttt gaaggaaccc atgcatggcc
     2281 gcctaaagaa gtgctttccg aagctttcac ctggatggag cttcatgcca tgagagataa
     2341 gaaaaagccc gaagataaag actatattaa caataaactg tcggaataca ccgggcagct
     2401 cgacgaagta aaaaaatccg gtaacaagta caaggaatat ttgctgacaa aaaagatggt
     2461 tacctatttt aaaggattgg ctgatattgc agccctggaa gaatcgctca aaaaacttca
     2521 ggacaatgcc gcggtaaaaa gcgcaatcga caacgccatc ctcaatctga aaaaagaact
     2581 tgccatgcag cagaaatatg gcccaaaaat aagaactgaa agtgctgcct ggtggaaaaa
     2641 tgaagtggcc tacctgaaca aattcattgc tatgtcgcag aatgaagatg aaaaacatat
     2701 tgtaaaaagg gtgctagaat acctcagtct ttcggctttt tcggcaagct cttcactgta
     2761 taaccaaaat aaatttgctg atgcggaaca ttatattgaa atttattcta tcatagacag
     2821 cgacaatccc gaacctgcat ttatgaaagc acaactggca gcacgcaaaa aagatgtcga
     2881 tcaaaccctg gaacaactta aaaaagcagc atccctcggc tttgccgaaa ggtctcgcgt
     2941 ggataatgat actattttta tgaaatttaa agagaaaaaa ggcttttcaa atattatgga
     3001 tctcatctcc gaaaataaga ataagaaata gtcttttttc tgtaatcccg tttttttttc
     3061 gctccattta acattgcttt gttatgccga aaattgtttt tgaagtttgt gccaattcgc
     3121 tgtcttcggc cctggctgcc agggatggag gcgcccaccg tattgaactc tgttccagtc
     3181 ttaactccgg cggtcttaca ccctctgcag gtacgataaa atcagtttta tcattggtgg
     3241 atattcctgt ctttgtgctg attaggccgc gggccggtga ttttctttac agccgcaatg
     3301 agtttaatgt aatgaaggaa gatatcaaat ttgcaaaaaa ccttggggct gcaggtgttg
     3361 tctttggaat attaaaccct gaagcaggta ttgatgttga ccggtgttcc gaactggtaa
     3421 gtctggcaaa acctatgcaa tcaacatttc accgggcttt cgacagggta atggatccca
     3481 actcggcact ggaagatgta ataaaaaccg gagcccaacg cgtattgact tccggtttaa
     3541 atgaaaaagc agtgaaaggc aaggagttca ttaggaaact ggtagaacag gccaacggca
     3601 ggatagccat aatggccgga agaggcatta atccacaaaa tgttgcggaa atagtgtccg
     3661 acaccggtgt aaaagaaatt cacgcttcct gctcagcctc cgtatacagc aagatgatct
     3721 ggctcatcag attttcttcc tttgacctgg attactgtca tacagtgacg aatgcctgtg
     3781 ccgtcaggaa aatgcttgat gttattaata aagtagattg ttgattatgg gaaaaaaatt
     3841 actgttgtcc attgctgcat tgttttgtgt cctaatggtc atgggacagg acagatcgcg
     3901 gcattttatt aatgacactg cataccgcgg acaggtggaa aaagatttcg tgaaacaaca
     3961 actgattgcc cggggcaggg ccgcgcaatt gtttaaagta tttgataata atctgtcact
     4021 ggaagaagag gaagccttga aatttcttta tgcgtatatg ccgttgagtg atttggccga
     4081 ttatgacgga aatttttttc tggggcaggt acgcatgtcg ctgctggcca aaaagacgtt
     4141 cagttgggga gacaaaattt ccgacgacat tttcaggcat tttgtgctgc cttaccgggt
     4201 caacaacgaa aacctcgatt cggcgcgcac ggtaattttc ggcgaattga aagaccgcat
     4261 aaaaaatctt tccatgcatg atgcggcatt ggaagtgaac cactggtgcc acgagaaagt
     4321 gagctataag cctactgatg gccgcaccag cgcccccctg agcactatac ggacggccta
     4381 cggacgctgc ggcgaagaat ccacgctggc agtgaccgct tttcgagcgg taggcattcc
     4441 ggcacggcag gtgtacacac cgcgctgggc acattgcgac gacaaccacg cctgggtgga
     4501 agtgtgggtg gatggaaaat ggcattttct gggcgcctgc gaacccgaac ccgacctcga
     4561 tattgcctgg tttgcggctc ccgtgctgag agccatgctc tgcaacacca ccgtattcgg
     4621 aaagtaccgg gctgccgaag aatccctcaa aagtgatgat caatttactc agattaatct
     4681 gatagaaaac tatgcccctg caaaaaaact gtttgtgaaa acggtggata caaattcccg
     4741 ggctgttgcc ggtgctgtgg tggagtttca actgtataac tatgcggagt tttatccgct
     4801 ggcaaaaaaa aataccgacg atcagggact tgcctttcta acaaccggcc tgggcgatct
     4861 gcttatctgg gcatacaaag gtgatacttt cggctatata caagtttccg taggaaagac
     4921 cgatacggct gtcgtggtac tgaataaatc cgctattgaa aacgatgagg tgatttttac
     4981 cctggtgcct cccattgaaa gacaacccaa agtggctgaa gctaaaggaa aaaaagaaaa
     5041 tgaaaaacgc ctgcaaaatg aggataaaat cagaggcgct tacgaagcag gatttattga
     5101 ttcggtatca agctgccggt ttgcgcaaaa tctccagctc accaccgaca cggtgtggat
     5161 gtttttaaag caaagccgtg gcaactggca cg
//
LOCUS       DAOSFP010000148         5191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14209_length_5191_cov_4.229945, whole genome shotgun sequence.
ACCESSION   DAOSFP010000148 DAOSFP010000000
VERSION     DAOSFP010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14209_length_5191_cov_4.229945"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(354..611)
                     /locus_tag="PLI16_05510"
     CDS             complement(354..611)
                     /locus_tag="PLI16_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOH84052.1"
                     /translation="MKYTVYVLKSLSTGKLYTGQTEDIKRRLNEHQTGTGPVRYTKGR
                     GPWELVYTEEYDERSQAMKREKYLKTGAGRDFLHRIIQSKG"
     gene            complement(652..725)
                     /locus_tag="PLI16_05515"
     tRNA            complement(652..725)
                     /locus_tag="PLI16_05515"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(689..691),aa:Asn,seq:gtt)
     gene            complement(848..2197)
                     /locus_tag="PLI16_05520"
     CDS             complement(848..2197)
                     /locus_tag="PLI16_05520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH84053.1"
                     /translation="MFRSHKLQLDALEKRTFRIHFAYSIIEGIILGVLAINEFVFLKS
                     LGGSDIGVSILFQFSVFVLTFSVLLNEWSRRIKNAKRFLITLGILTRAPLLLLFFFPN
                     QVSTLPGNQVYHYIFLGIFLVYYFANPIIYPVINQFLKTNYFHHNFSILYSWSTMANK
                     IVMLLVTFVFGWLLDQNQENYHYVYPVMALLGISSVWLLSRLADPDTQVTKPAVPLLQ
                     SIKVTISNMSQKMRSNKPFRDFQSGFMFYGFAFMGTVSVIAIFFQKELELNYFSVAFY
                     KNSYNILAIFLLPLFGRLMGKIDPRKFAAITFASLLLYLFFIALTSFAPWFMWIQGLK
                     IYYSLLAAMIFNGVFAATMGLLWSIGSAYFCPEDEVAEYQAIHLTFTGFRSFFAPVLG
                     LGIYLVVGYTATFVTGMILLLIAVIISLRSYKKYRLKPMSRAKTQADMVSAEMKSGA"
     gene            complement(2342..2980)
                     /locus_tag="PLI16_05525"
     CDS             complement(2342..2980)
                     /locus_tag="PLI16_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84054.1"
                     /translation="MVFLFPLAGMSQENFGIAFIRGFNPVIDVFGFETVTIIKKKKQY
                     ELESKSNIKATGVFVFEDYKLISAGFTDKKGLNLTGGTFENGEGELSLTYKNKQQITA
                     NFTGGLLNDTACYYEYVNGIKVLMNKINFKSGYLDGTCYSYDNEGNMLTQTKYEHNKM
                     VYRAWYGFYPESGPFKPMVHKGKIRQEDFYENNKIKRTVIYKTDGTVDRIIE"
     gene            3118..>5191
                     /locus_tag="PLI16_05530"
     CDS             3118..>5191
                     /locus_tag="PLI16_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84055.1"
                     /translation="MIRNKKSIVYFIVLLALTAAFLYGFYGKIIIHPNAFIFGNHGDG
                     MKNYYTYAYYIQHNQSYTNFEGMNYPYGENFTYTDCHPLLAFVLKLVHEVFPGISQYS
                     IGILNLLMIFSIGLTAIILYFLLRELKVTRLLSVLGAVAIAAMSPQIFRLSGHYALSY
                     SFFIPLTIYLILLFEKGRHRLRILIMLSLSIFFFFAIHAYLGMIAATLVFCYVALGLA
                     GNLFRREKGALVNHLWLLAAALIPVALYYLFVKITDIHTGRTTNPWGILENHAELSSV
                     FLPVCSPFDKIKGSLFPGLIQPWEGWSYIGIITILALIAFIVASVVSSVKKRRPALNK
                     TWVESYPLRLLFAASLIILAFSLFVPFRWFGWEHLINYFDVIKQFRAIGRFAWVFYFV
                     SSIVLIVITNSMFNGLRKKNLTLPAYFLAVPIPFLLFAESWKYHEITSADIVKSPNFF
                     CAQQLPQPLKHDIENTNVFGYQEILPFPFFYIGSENYGKVADEKVYRLAFLFSYHTGL
                     PMISSYLTRTSIHESKNIMQLLASNFYQKKIRDDLPVNKPFLLVCLNGYLNSKEAEYV
                     KKATLICARDDYSLYEINPEVFFENTAKSEFSAFEEIKDSLYEKQGFLVSDTSLIFAY
                     DDLANEGTDIAFSGGRGCHFGPQKDFHILLKFEKGVLPLNHKFIARLWMYNDGDNFGQ
                     DCLNGMFFF"
BASE COUNT         1472 a         1107 c         1058 g         1554 t
ORIGIN      
        1 ttttcttttg ataacgaaat gtcaattttc tcgaatccca tagttttgtc tttcaatgcg
       61 cgctaacgat tgtgctgccg taagtccgtt tacaaacgct aataaacatt tgtaaacggt
      121 ttcaaatata aagatttatc ctgcacaggc aaagaaaaaa atattttaca tagaaaatca
      181 tatggtattc caatatggcg gttaccaacc tgctctaaaa tggctaaaag accgcaaagg
      241 ctgatctgaa ctgtaaataa cagggcgcca ttgatcacaa ataaaaaaag ccgcttttgc
      301 ggctttgctc cccagcctgg acttgtccgc cgcggcggac agggaccctc tgattaccct
      361 tttgattgaa taatacgatg taaaaaatcg cgaccagccc ctgttttcag gtatttctct
      421 cttttcatag cttgcgaacg ttcatcatat tcttctgtat ataccaattc ccaaggcccg
      481 cggcccttgg tatagcgtac tggtcccgta ccggtctgat gttcattcag ccgccttttt
      541 atatcctctg tctgacctgt atagagtttc ccggttgaaa ggctctttaa gacatataca
      601 gtgtatttca tattcaaaca aaaaagccgt ctttcaacgg cttcattttt gctccccagc
      661 ctggacttga accagggacc ctctgattaa cagtcagatg ctctaaccaa ctgagctact
      721 gaggaatttt taaaattgga gtgcaaattt agctattttt ttgatacatc aaaatttttt
      781 ttaaaaaatt ccacggctgc ggattttcat gcacaacgaa aaacaggccc ccgagcagaa
      841 cagactttta tgcgcctgat ttcatttcgg cggacaccat atctgcctgc gtttttgccc
      901 ggctcatggg tttcaggcgg tatttcttat acgacctcaa cgaaataata acggctatca
      961 acagcaaaat catgccggta acaaaggtgg ctgtatagcc taccaccaga tagatgccca
     1021 aacccaatac gggggcaaaa aacgaacgaa aacccgtaaa agtaagatgt atggcctgat
     1081 actcggctac ctcgtcttcg ggacaaaaat aggccgagcc gatactccag agcaaaccca
     1141 tggtggcagc aaaaactccg ttgaaaatca tggccgccag cagcgaatag taaattttta
     1201 atccctgtat ccacataaac cacggcgcaa aggaagtgag cgcgataaaa aataaataca
     1261 aaagcaggga ggcaaaagta atggcagcaa acttgcgcgg gtcaattttt cccatcaaac
     1321 ggccaaataa cggtagtaaa aaaattgcca gaatgttgta agagtttttg taaaaggcaa
     1381 cactgaaata attcaactcc agctctttct gaaaaaatat ggcaatcacc gagacggtac
     1441 ccataaaagc aaatccataa aacataaacc ctgactgaaa atcgcggaag ggcttgtttg
     1501 agcgcatctt ttgactcatg ttggaaatgg tgacttttat ggattgcaat agcgggacgg
     1561 caggttttgt tacctgcgtg tcgggatcgg ccaaacgcga tagaagccaa accgatgata
     1621 tgcccaataa ggccatcaca ggatacacat agtggtagtt ttcctggttc tggtcgagca
     1681 accacccgaa aacaaaggtt accagcagca tgacaatctt gttggccatg gtggaccagc
     1741 tatacagtat gctgaaattg tgatgaaaat aattggtctt gagaaactgg tttataacag
     1801 ggtagatgat ggggttggca aaataatata ccaaaaaaat accgagaaaa atataatgat
     1861 aaacctggtt tcccggcagt gtgctgacct ggttggggaa aaaaaacagc aacaacaatg
     1921 gtgccctggt aaggattccc agtgtaatta aaaaacgttt ggcatttttt atcctccgcg
     1981 accactcgtt gagcaacacc gaaaatgtca acacaaaaac actgaactga aacaatatgc
     2041 tgactccgat gtcgctgccg ccaagacttt tcagaaaaac gaactcgttg atagccagca
     2101 ctcccagtat tatcccttca attatcgaat aggcaaaatg aatgcggaag gtgcgttttt
     2161 cgagtgcatc gagttgaagt ttatgggacc ggaacatcag agattttttg tgatgcaaaa
     2221 atatgcgata ttaaagaaac ccctgaagat tctatcaaag aatttttttg acggagggtt
     2281 gtgaaagaca gatggaaatg ttgcgggagg aacaaacgtg aaaaggcggc attcggcttg
     2341 cctattcaat aattcgatct accgttccgt ctgttttgta tatcactgtg cgtttgattt
     2401 tattgttttc ataaaaatct tcctgccgga tctttccttt gtggaccatt ggtttaaaag
     2461 gaccggattc cggataaaac ccataccatg ccctataaac cattttatta tgctcatatt
     2521 tggtttgcgt cagcatgttg ccctcgttat cataagaata gcatgttcca tccaggtagc
     2581 cacttttaaa atttatttta ttcatcagca cctttatacc gttcacatat tcataataac
     2641 acgcggtatc gttgagtagg ccgccggtaa aatttgcggt tatttgttgt ttgtttttgt
     2701 aggtcaggct caactcgcct tcgccatttt caaatgtgcc accggtcagg ttcaaccctt
     2761 ttttatccgt aaaaccggca ctgataagtt tgtagtcttc aaagacaaaa actccggttg
     2821 cctttatatt tgattttgat tccaattcat attgtttctt tttcttaata atggtaaccg
     2881 tctcaaatcc gaacacatca ataaccgggt taaatcccct gataaaggct atcccgaaat
     2941 tttcctgtga catgccggct aaaggaaata aaaagaccat tatgtaaaaa agctttttgt
     3001 tcatttcatt tgctgacgga tcaactacgc aaaattacat gaatttcaaa agagctttca
     3061 taaacagaac aaatacaaac atttttttat cctacatttg cactataatt tttatgaatg
     3121 ataagaaata aaaaatcaat agtttatttc attgttttac tggcactgac cgcggctttt
     3181 ctgtatgggt tttacggaaa aataatcata catcccaatg cttttatttt tggcaaccac
     3241 ggcgatggaa tgaaaaatta ttacacctac gcctattata tccaacataa ccaaagctac
     3301 accaacttcg aggggatgaa ttatccttat ggcgaaaatt ttacctatac cgattgtcat
     3361 cccttgctgg catttgtcct taaactggta catgaggtct ttccgggcat atcgcaatac
     3421 agcatcggta ttctgaacct gctgatgatt ttttccatcg gcctcaccgc tataattctt
     3481 tattttttgt tgagagaact gaaagttacc cgcctgcttt cagttctggg cgctgtggcc
     3541 attgcagcaa tgagtcctca gattttccgg cttagcggac actatgccct gtcgtacagt
     3601 ttttttatcc cgctgacgat ctatttgata ctgctttttg agaaaggacg ccatcgcctg
     3661 aggatattga ttatgctgtc gttgagcatc tttttctttt ttgccataca tgcctacctt
     3721 ggtatgattg cggctacttt ggtgttttgc tatgtggcat tagggttagc cggtaattta
     3781 ttcagacggg aaaaaggtgc cctggtaaac catctctggc tgttggccgc tgcgcttatc
     3841 cccgtggcgc tgtattatct ctttgtaaaa atcaccgata tacatacggg gcgtacaacc
     3901 aacccctggg ggatactcga aaaccacgct gaattgagca gtgtcttcct ccctgtatgt
     3961 tccccgtttg ataaaatcaa agggagcttg tttcccggtc ttattcagcc gtgggaggga
     4021 tggtcgtaca taggaataat aaccatactt gcgctgatag cttttattgt tgcatctgtg
     4081 gttagttcgg tgaaaaaaag gcgccccgcc ctgaataaaa catgggttga aagttatccg
     4141 ttgagattgt tgtttgctgc atcgctgatt attcttgctt tttctttgtt cgtacccttc
     4201 cggtggtttg gctgggagca cctgatcaat tattttgatg tcattaaaca attccgcgct
     4261 atcggccggt tcgcctgggt gttctatttt gtatcgtcta ttgtgcttat tgttatcact
     4321 aacagcatgt ttaatggact taggaagaaa aaccttacgc ttcctgcata ttttcttgcg
     4381 gtaccgattc cctttctttt gtttgctgaa agctggaaat accatgaaat tacttcggca
     4441 gacatagtga aatctcccaa tttcttttgc gcacaacagc ttcctcagcc gctgaagcac
     4501 gacatcgaaa ataccaatgt ttttggctat caggagatat tgccctttcc ttttttctac
     4561 attggctccg aaaattatgg caaggtggcc gacgaaaagg tgtatcgcct ggcgtttctt
     4621 ttttcatacc atacgggctt gccgatgatt tcgtcttatc tcacacggac ttccatccac
     4681 gagagcaaaa atattatgca gttgctggca tcaaattttt atcagaaaaa aataagagat
     4741 gatttgcctg tcaataaacc ttttttactt gtttgtttga atggttacct gaacagtaaa
     4801 gaggctgagt atgtgaaaaa agccacgctg atttgtgccc gtgacgacta cagtctttac
     4861 gaaatcaatc cggaggtatt ttttgaaaac accgctaagt cagagttcag cgcctttgag
     4921 gaaataaaag atagtctgta cgaaaaacag gggtttcttg tatccgacac ctcgttgatt
     4981 tttgcatatg acgaccttgc caatgaaggc accgatattg ctttttcggg gggcagggga
     5041 tgtcatttcg gcccccaaaa ggacttccac attttgttga aatttgaaaa aggcgtattg
     5101 ccgctgaacc ataaatttat cgcgcgttta tggatgtaca acgatggcga caattttggg
     5161 caggattgtc ttaatggaat gtttttcttt c
//
LOCUS       DAOSFP010000149         5165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14308_length_5165_cov_4.557339, whole genome shotgun sequence.
ACCESSION   DAOSFP010000149 DAOSFP010000000
VERSION     DAOSFP010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14308_length_5165_cov_4.557339"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..199)
                     /locus_tag="PLI16_05535"
     CDS             complement(29..199)
                     /locus_tag="PLI16_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84056.1"
                     /translation="MKPKLYNLPVYDANPGRPQMLRVIRCEKFTRIDFGHQADSYYVR
                     GGWVNIFPKTFI"
     gene            complement(180..1217)
                     /locus_tag="PLI16_05540"
     CDS             complement(180..1217)
                     /locus_tag="PLI16_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOH84057.1"
                     /translation="MSLGKEAYIRYKVINECLTNRFATYPTIFDIIDACENKLGKTFS
                     VSAIQKDIKAMKEDEALGFLAPIKFSRQYNGYYYAHPDYSINSIPLNSSELEALEAVS
                     DVLQTFAGNRISENFNLAVEKIFTTLKEKKCQIGKKQRYIQTDTNQRQKGFENFERLF
                     HAISNKIPVCFVHYSYNKRCFNSVVTHPFLLKEFQNRWYLVAYSEQHKCLRTFGLDRI
                     RDPLLLDKPFHHVDDKVVDAYFVHIYGTYPWTGCKRQKIFFYASALMSEYIRANPVHE
                     SQKEHEIQPDGGVIFVVELIPSQELVNFFKQFSPDVVVLKPAEMVNAVESALKTAFFR
                     YRKNHHETQTL"
     gene            1322..1870
                     /locus_tag="PLI16_05545"
     CDS             1322..1870
                     /locus_tag="PLI16_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84058.1"
                     /translation="MEKEIISYARKELGYEKFIPIFRKKNSRDFVTENIVIIAERYNK
                     KILYISRLCMGSIVLSGLAMVASAPLMHSDIRLAAVLFSAGVSLAGPMLMLTGMISKQ
                     LKEFAWIDVDGAREGVKSDKVNLVLREVNDYYKNKGKMPEIFEILIVSEGGIDAGARA
                     IHLKNKIRFFEKSKTGNSLQEI"
     gene            complement(1881..3182)
                     /locus_tag="PLI16_05550"
     CDS             complement(1881..3182)
                     /locus_tag="PLI16_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH84059.1"
                     /translation="MNKVCGFLLGLFLTTLGFAQGIYHIKPENADCKKPVEIKDTIFG
                     PTNPPSGHGELMEISGAKNSLYEFEKEHNTCWYFFKAKYDAVLEMEIIPVNIRDDYDF
                     ILYRYSGRNFCLDVSDKKIIPVRSCISRNDKALKSRTGLSNEAPEEYIHSGPGASFSK
                     SLRVKKGEIYYLVVDNVYPQGGGHTVILHYSGYDKKAEPVKPPEKTANKTKTKPPQNT
                     TPKNVAVTINTIDKDSKKAVASNIKVYYKDKPALPPAAASDSVITYVAELAPSTAYNI
                     KTEAINYFDNATSIKTNADGQSMSVTIELTRIKTGQNVVFENILFYGNTAEILPESKP
                     ALEAIYSTMKRYPALHIEIQGHVNCPTTWEECSKMKEHNMQLSVNRAKAIYDYLEDGG
                     IDPQKMTFKGFGETKMIYPDARSEDKMKKNRRVEILVTAVE"
     gene            complement(3187..>5165)
                     /locus_tag="PLI16_05555"
     CDS             complement(3187..>5165)
                     /locus_tag="PLI16_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOH84060.1"
                     /translation="YFSADSSFAFNLGVGFAAVPHNYGRVWFPCIDDFVDRATYDCFI
                     TVKNDKKAICGGTLMSVIDNGNNSSTYHWRMHNTIPTYLASVAVGNYVAVNDTFHGMS
                     GPVPINIYVRPQDTNAAKGSFINLKQILAIFETRFGPYRWERVGYVGVPFNSGAMEHS
                     TNIAYPLACITGNLAYESLYAHELSHQWFGDLTTCSTAADMWINEGWATYCESVYQEL
                     LYGSEAYRLNVSENHYNVLFSAHEEDDGYRALYGIPDEYTYGTTVYDKGADVVHTLRN
                     YLGDSVFFAVITSLLDDYRFRDISSAGLRDYLSVHTGINMDDFFQTWVFSPGFPHFAV
                     DSFKVLQTSPEVLVKVWVRQKLNHAPAFANSNKIELTFGKNDWTFFSDTLHFSGETGT
                     KEFVLPFVPDFVMMDYHEKISDAITDMTKVIKTTGPYDLTRALCRVDVTNISDSVLLR
                     VEHNWVAPDTLKTPRPDILRLSDYHYWKVDGIFPGDFVARARFKYNRSVSADVGNTDS
                     GLLPVAASTDSLLLLYRPDVSSDWSIAHFTHLGPASVGFLIVDTLRRGEYTFAVGTPY
                     FAAINKIPQENGLNVFPNPSNKTFTFSFGITEDAAIMIYDASGKEVCMIRIEKNEKQA
                     VWDAGNCEPGTYYAHLISWKGKKKSGDKKLLLVK"
BASE COUNT         1373 a         1122 c         1210 g         1460 t
ORIGIN      
        1 gaaacatata taaaattaat gcctgacttt aaataaatgt tttcggaaaa atattcaccc
       61 atccgcccct gacataatac gagtcggcct gatgaccgaa atcaatgcgg gtaaacttct
      121 cgcagcgtat tacccggagc atttgcgggc gtcccgggtt ggcatcatac acgggcaggt
      181 tatagagttt gggtttcatg atgattcttt ctgtaacgaa aaaaagccgt tttcagggcg
      241 ctttctacgg catttaccat ctcggctggt ttcagcacaa ccacatccgg actgaattgt
      301 ttaaagaaat tgaccaactc ctgcgaaggg atcagctcaa ccacaaaaat cacacctccg
      361 tccggttgta tttcatgctc tttttgcgat tcgtgcaccg ggttggccct aatgtattcc
      421 gacattaaag cgcttgcata aaaaaagatt ttttgccgct tgcatcccgt ccatgggtag
      481 gtgccgtaaa tatgtacaaa ataggcatcc actaccttgt cgtccacatg atgaaagggc
      541 ttgtcaagca acaaagggtc gcgtatgcgg tcgagaccga aggtgcgcag gcatttatgc
      601 tgttcggagt aggccaccag ataccagcgg ttctggaatt cctttaacag aaaaggatga
      661 gtaaccaccg agttaaaaca tcttttatta taggaataat gtacaaaaca cacaggtatc
      721 ttattgctga tggcgtgaaa caggcgttca aagttttcaa aacctttctg ccgttggtta
      781 gtgtctgtct gaatgtaccg ctgttttttg ccgatctgac atttcttctc tttgagtgtg
      841 gtaaaaatct tttcaactgc caggttgaaa ttttcgctga tacggttgcc ggcaaatgtc
      901 tgtaatacat ccgacacggc ttcgagggct tcaagttccg aactgttcaa cgggatgctg
      961 ttgatagaat aatcagggtg ggcatagtaa taaccgttgt actgcctgct gaactttatt
     1021 ggagccagaa aacccagtgc ctcatcttct ttcatggctt tgatgtcttt ttgaatagcc
     1081 gaaaccgaaa aagtttttcc tagtttattt tcgcaggcat caataatgtc aaagatggta
     1141 ggataggttg caaaacggtt ggtgaggcat tcgtttatga ccttgtaacg gatataagct
     1201 tcttttccca gcgacataat aatgcaaata tataataaat atttaaaacg caaattgact
     1261 gcgtttgaga aaaatatttt tgtaacaaat caaaggaaga aaagtgacaa gacgaattaa
     1321 aatggaaaaa gaaatcatca gttatgcaag gaaggaactg ggctatgaaa agttcatccc
     1381 gatattcagg aaaaagaaca gccgtgattt tgttacagaa aatatcgtga ttattgccga
     1441 aaggtataat aaaaagatat tgtacatttc aaggttgtgt atgggtagca ttgtgctaag
     1501 cgggctggcg atggtcgcat ctgccccgct gatgcatagt gatatcagac tggcggctgt
     1561 gttgttcagc gcaggtgttt cacttgccgg gcctatgttg atgcttacag gaatgatatc
     1621 aaagcaactg aaagaatttg cctggattga tgttgacggc gcccgcgagg gggttaaatc
     1681 tgataaggtt aatctcgttc tccgtgaagt taatgattat tataagaata aagggaaaat
     1741 gccggaaata tttgaaatcc tgattgtttc agaaggaggg attgatgccg gtgcccgtgc
     1801 gattcatctg aaaaacaaaa taaggttctt tgagaaaagc aaaacaggta acagcttaca
     1861 ggaaatatga aaaccgggga ttattctacg gcggtaacca aaatttccac ccggcggttt
     1921 tttttcattt tatcttcgct gcgggcgtca ggatatatca tttttgtttc gccaaaccct
     1981 ttgaaagtca ttttttgcgg gtcaatgccg ccgtcttcaa ggtagtcgta aatggctttc
     2041 gcacggttta ccgataattg catgttgtgt tccttcattt tgctgcattc ttcccaggta
     2101 gtcggacaat tcacgtgccc ctgtatttcg atgtgcaggg cgggatacct tttcatagtg
     2161 gaataaatag cttccagggc cggtttcgat tcgggcagga tctcggctgt attgccgtag
     2221 aataagatgt tttcaaacac cacattttgt cctgttttta tcctggtcaa ttcgatggtt
     2281 acactcatgg actgtccgtc ggcgttggtc ttgatactgg tagcattgtc aaaataatta
     2341 atggcttcgg tcttgatgtt ataggcggtt gacggagcca gttcggcaac gtatgttatt
     2401 acactgtcgg aagcagcggc aggaggaagg gcaggcttgt ccttataata aaccttgatg
     2461 ttggaagcta cggccttttt gctgtcttta tcaatggtat taatagtaac ggcgacattt
     2521 ttcggtgtcg tattctgagg cggtttggtc tttgttttgt ttgcggtttt ttctggtggt
     2581 tttacgggtt ctgctttttt gtcatagccc gaatagtgca gtatcacggt gtgaccgccg
     2641 ccctggggat acacattatc caccaccagg taatagattt ctcctttttt gacccgcaaa
     2701 ctcttactga acgaggcacc cgggcccgaa tgaatgtact cttcaggggc ctcattcgac
     2761 aagccagtcc tgcttttcag ggctttatca ttacgcgaga tgcaactgcg taccggaatt
     2821 atttttttgt cgctcacatc caggcaaaag ttacggccgc tatacctgta aagaataaaa
     2881 tcataatcat ccctgatatt tacgggaatt atctccattt ccagcacggc atcgtatttt
     2941 gccttaaaaa aataccagca ggtgttatgt tctttttcaa attcgtacaa actgtttttt
     3001 gcgccgctga tttccatcaa ctctccatgt cccgaggggg gattggtagg accaaaaatg
     3061 gtatctttga tttccacagg ttttttgcag tcggcattct caggttttat atgatatatg
     3121 ccctgggcaa atcccagtgt cgtcaaaaac aatcccaaca gaaatccgca gactttattc
     3181 atgttgttat ttcactaaca gtaatttttt atcacctgat tttttctttc ctttccagga
     3241 aatcagatgg gcataatacg ttcccggctc acaattgccc gcatcccaaa cggcctgttt
     3301 ttcatttttc tcaattctga tcatacacac ctctttgccc gatgcatcat aaatcattat
     3361 tgccgcatct tctgttatac caaacgaaaa ggtgaaggtt ttatttgaag gattgggaaa
     3421 tacattcagg ccattttcct gagggatttt attaatggcg gcaaaatagg gtgtgccaac
     3481 ggcaaaagta tattcgcccc tgcggagggt gtccactatc agaaacccaa cagaggcagg
     3541 tcccaaatgt gtaaaatggg caatactcca atcggaagag acgtcaggcc ggtacagcag
     3601 gagcagggag tcggtagaag ctgctaccgg caacaggccg ctgtcggtat tgccaacgtc
     3661 ggcgcttacc gaacggttgt atttgaaacg tgcccgggca acaaaatcac cgggaaaaat
     3721 accatccact ttccagtagt gataatccga caggcggaga atatcaggcc tgggagtttt
     3781 cagggtgtcg ggggctaccc agttgtgttc cacgcgcaac aacaccgaat cactgatatt
     3841 tgttacatcc acccggcata aggcacgggt aaggtcgtac ggccccgtcg tcttaatcac
     3901 tttggtcata tcggtaatgg catccgaaat tttttcatgg tagtccatca ttacaaaatc
     3961 aggcacaaag ggcaacacaa attctttggt tccagtttct cccgaaaaat gcagggtatc
     4021 cgaaaaaaat gtccagtcgt ttttaccgaa ggtcagttct atcttgtttg aattggcgaa
     4081 agccggagca tggtttagtt tctggcgtac ccacaccttc accaatacct ccgggctggt
     4141 ttgcagtact ttaaatgaat ccaccgcaaa atgcgggaat cccgggctga agacccatgt
     4201 ctgaaaaaaa tcgtccatat tgatgccggt gtgaacgctt agataatcgc gcaggccggc
     4261 ggaggagata tccctgaaac ggtaatcgtc cagcagggaa gtgatgacgg caaaaaacac
     4321 gctgtcgcca agatagtttc tgagggtatg taccacgtcg gcgcctttgt catacactgt
     4381 tgtgccatag gtgtattcgt caggtattcc atacagggcg cggtatccgt catcttcttc
     4441 gtgggccgaa aacaacacat tataatgatt ttcactgacg ttgagcctgt aggcttcgct
     4501 gccatataac aactcctggt aaaccgattc gcagtaagtg gcccagccct cgttgatcca
     4561 catgtcggcg gcggtgctgc acgttgtcaa atccccaaac cactgatgcg acaattcgtg
     4621 ggcatacaac gattcatagg ccagattgcc tgtaatgcag gcaagcggat aagcaatatt
     4681 ggtggaatgt tccatcgccc cgctgttaaa aggcactccc acatagccaa cacgttccca
     4741 gcgatacggg ccgaaacgtg tttcgaagat cgccagaatt tgtttcaggt taatgaagga
     4801 tcccttagcc gcattggtgt cctggggcct gacgtagatg tttattggca cagggcctga
     4861 catgccgtga aacgtgtcgt tgacagccac ataattgcca acagcaaccg aagccaggta
     4921 ggtggggatg gtattatgca ttctccagtg ataggtgctg ctgttgttgc cattatcaat
     4981 aaccgacatc agggtgccgc cgcagatggc ttttttatcg ttttttactg tgatgaaaca
     5041 atcgtaggtg gccctgtcaa caaaatcatc aatacatggg aaccaaactc tgccgtagtt
     5101 gtgtggaact gcggcaaagc ccacgccaag gttgaaggca aaagaactgt cggcgctgaa
     5161 ataaa
//
LOCUS       DAOSFP010000150         5103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14549_length_5103_cov_4.406300, whole genome shotgun sequence.
ACCESSION   DAOSFP010000150 DAOSFP010000000
VERSION     DAOSFP010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14549_length_5103_cov_4.406300"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..1785)
                     /locus_tag="PLI16_05560"
     CDS             complement(295..1785)
                     /locus_tag="PLI16_05560"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH84061.1"
                     /translation="MNKKFITNLIILLFLNLLIKPFWILGIDRSVQNAVGAQEYGLYF
                     AIFNFTFLFSILLDMGITNFNNRNIAQNHHLLNKYFSSIVILRFFLAILYSVVTLSAG
                     LLVGYDAYQMAMLAILCFNQFLISFILYLRSNISGLHYFKTDSIISVLDRFLMIIICG
                     ILLWTNITGHTFKIEWFIFTQTFAYLVTAFVAAVIVIKKAKFQKLKWNFPFFVLIIKQ
                     SFPFAILVLLMAFYNRIDSVMIERILPGPEGGQQAGLYASAYRLLDAVNMMAYLFSVI
                     LLPLFSRMIKLKENIERLAQLSFTLLFLLSVTIALGSFFFRTEIMTMLYPLHPAETPL
                     EYTARITDTSWIFGILMFGFIPISTTYVFGTLLTANGNLKYLNIMAGCGMAFNLTFNF
                     ILIPKFEAIGSAYVSLTTQSLTSLAQVLLAVWIFKFKMNINYLVKLLIFVLITAITGY
                     FSYIVYQKFQIDWKLMLMVIAMTAFAAGIFTRLLDFRAFIQMLIRK"
     gene            complement(1805..2542)
                     /locus_tag="PLI16_05565"
     CDS             complement(1805..2542)
                     /locus_tag="PLI16_05565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOH84062.1"
                     /translation="MKNTIKVILQKLLGFDRYLYIFSIYIINTLKWNRKEGDFIFFRN
                     MVPNGGLILDIGANIGVMSIHLARTHKDSNVIAFEPMPDNIKCLKKIIRHYGLNNISL
                     IETALGERAGTIEMVMPTQGSARLQGLSHVIHESITDNNTGNRVVTPLTTLDDFFQQH
                     PGSKPVTAIKIDVENYEYYVLKGAVSLLQRCKPIIYCELWENKNRDDCFSLLSTLGYE
                     IRVLDKKHLSPYNPSIHKTQNFFFIPK"
     gene            complement(2539..2742)
                     /gene="thiS"
                     /locus_tag="PLI16_05570"
     CDS             complement(2539..2742)
                     /gene="thiS"
                     /locus_tag="PLI16_05570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOH84063.1"
                     /translation="MNITLNNNKEVIDADHLTVAELLKIKNFTFKLLIIKINGKVVSQ
                     MDYAHTSISEGDDVIVLHLISGG"
     gene            complement(2747..3334)
                     /gene="thiF"
                     /locus_tag="PLI16_05575"
     CDS             complement(2747..3334)
                     /gene="thiF"
                     /locus_tag="PLI16_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOH84064.1"
                     /translation="MDFNKIKSILSAKTVGIAGCGGLGSNCAVALARVGIGKLILCDF
                     DAIEPGNLNRQYFFYNQLGMKKATALKENIQNINPEVIAEDHIVKLDPEIIMKLFAGC
                     DVIVEAFDRADMKKMILETVLCQMPDKPIVSGVGIAGWGNSNDIRMIQSGNLYVCGDG
                     HSEATENNPPLAPRVCMAANMQANTVLEILLGKNY"
     gene            complement(3414..4103)
                     /locus_tag="PLI16_05580"
     CDS             complement(3414..4103)
                     /locus_tag="PLI16_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOH84065.1"
                     /translation="MKIVVIGLGNFGSTLAMRLTQMGHEVIGVDKDMVKVQLLKDDVT
                     HAMCLDSTDIHSVKVLPLRDADVVVVSIGEDTGASIMTTALMKQLNVKRIISRAISKL
                     HVAVLEAMGIKEILHPEHETAIRLAKKLNLPGFVDSFEVSSDHNVIEIIAPQSFVGKT
                     LLDLNLTRKYNILVLTTIKVKSGINILGIESKKRIVNDVATSDTKIYDGDILLIYGAV
                     NDIKRMLKDEE"
     gene            complement(4115..>5103)
                     /locus_tag="PLI16_05585"
     CDS             complement(4115..>5103)
                     /locus_tag="PLI16_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOH84066.1"
                     /translation="EAAGALIIYTQLPENAGMSLSEKIFFSVFHSISGFCNAGISTLS
                     DGFQDLNFLYNYPFLITMSVLIIAGGLGFPIIFNFLKYLRHLIVNRIFRLNKHREYIH
                     KAWVININTRIVVVTTVIIILAGTLIVFIMEYNRSLQDHGWAGKLVTAFFNISSHRTA
                     GFNSIDFSVVQVPVIFLIMLLMWIGASPGSTGGGIKTTTFAISILNFISLAKGKDRVE
                     VFHRQVADSSLRRASAIIMLSFIVIGLAVFLVSFFDPEEKLLDVVFECFSAYGTAGLS
                     LGLTPNLSEASKLVISITMFLGRLGTLTVLIAFFKKIQHTNYHYPTENILIN"
BASE COUNT         1463 a         1027 c         1147 g         1466 t
ORIGIN      
        1 acacaatata atcagtatgt tataagtcta atttttttat tgtgtcgaag tcaggtgaat
       61 atgaaatcaa agcaggtgag gctgtaactg tcatggcttt atctgaatat atgtggtacg
      121 taatctacaa aatataaaaa acctcctggt ttgccaattg atggacagtt atctgatgca
      181 tttattcagg attaaatgta tttgaaaggt tgtttaaatt tgtacttttg gttttgaaaa
      241 tatgtacttt tggtttttgc gattatacat gctcaaaaaa aggcagggca ggcattattt
      301 tctgattagc atctggataa aagccctgaa atcaagcaaa cgtgtaaaaa tacctgcagc
      361 aaaagcggtc atggctataa ccatcagcat taacttccag tcgatttgga atttttggta
      421 cacaatgtat gaaaaatacc ctgttatggc agtgattaaa acaaaaatca ggagttttac
      481 caggtagttg atattcattt tgaatttgaa tatccataca gccagcagta cctgagccag
      541 tgatgtcagt gattgggttg tcaggctgac ataggccgag ccaatggctt cgaatttggg
      601 gataagaatg aagttgaagg tgaggttaaa cgccatgccg cagcctgcca taatgttgag
      661 atacttcagg ttgccgttgg ccgtcagcag ggtgccaaac acataagtgg tggatatggg
      721 gataaagccg aacatcagga tgccaaatat ccacgaggtg tctgttatcc gggcggtata
      781 ttccaggggc gtttctgccg gatgcagcgg ataaagcatg gtcatgatct cggtacggaa
      841 gaaaaatgaa cccagggcaa tggttaccga aagtagaaat aacagggtaa acgacaattg
      901 ggccagccgt tcgatgttct cttttagttt tatcatgcgc gaaaacaatg gcagcaaaat
      961 caccgaaaac aggtaggcca tcatgtttac agcgtcgagc agacgatagg ctgaggcata
     1021 caagccggcc tgttgccccc cctccgggcc tggcaaaata cgttcaatca taaccgaatc
     1081 aatgcggtta taaaatgcca taagtaatac caggattgca aaaggaaaac tttgcttgat
     1141 gattaataca aaaaacggaa agttccattt tagcttttga aattttgctt tttttatgac
     1201 aatgacagcc gctacaaagg cagtaacaag gtaagcaaag gtttgagtaa aaataaacca
     1261 ctctattttg aaagtgtgac cggtaatatt ggtccataaa agtatcccgc agatgataat
     1321 catcagaaag cggtccaaaa ccgaaataat actgtcggtt ttaaaatagt gaaggcccga
     1381 aatatttgag cgcaggtaaa gaataaaaga aataagaaac tgattgaaac ataatattgc
     1441 cagcatggcc atctgatatg cgtcataacc cacaagcaag cccgctgata aggtaaccac
     1501 cgaataaagt atggccagaa aaaaacgtaa tataacaatg ctcgaaaaat atttattgag
     1561 caagtggtgg ttttgagcta tgtttctgtt gttgaagttg gtaatgccca tgtcgagcag
     1621 tatgctgaac agaaaggtaa agttgaaaat ggcaaaatat aatccgtatt cctgggctcc
     1681 cacagcattc tgtacactcc tgtcgatgcc aagtatccaa aacggcttaa tgagcaaatt
     1741 cagaaaaagc aggataatca ggttggtaat gaattttttg ttcatacaga taactcgctg
     1801 gggattattt aggaatgaag aaaaaattct gcgttttatg gatggacggg ttgtatgggc
     1861 ttaaatgctt tttgtccagc acacggattt cgtaccccag ggtggacaga agcgaaaaac
     1921 aatcgtcgcg gtttttgttt tcccatagct cgcaatatat gatgggttta catctttgca
     1981 acagggaaac agctcctttc aatacataat attcgtagtt ttccacatcg attttaatgg
     2041 ctgtaacagg ttttgaaccg gggtgttgct gaaaaaagtc gtccagggtg gttagtggcg
     2101 ttactacccg gttgcccgta ttgttatcgg taatactctc gtggattaca tggctgagcc
     2161 cctgaagccg ggccgagccc tgtgtgggca tcaccatctc aatggttccg gcacgctcac
     2221 ccagggcggt ttcgatcagg cttatattgt tcaggccgta atggcggata atttttttca
     2281 ggcatttgat gttgtcaggc ataggctcaa aggcaataac attcgaatcc ttatgtgtcc
     2341 tggccagatg tatcgacatt acgccgatgt tggccccgat atcgagaatg aggccgccgt
     2401 tgggcaccat attcctgaaa aagataaaat ccccttcttt tctgttccat tttagtgtgt
     2461 tgatgatgta aatggaaaaa atgtaaaggt atctatcgaa gcccagtaat ttttgcagta
     2521 ttacttttat tgtgtttttc atccgccgct tatcaggtgt aaaacaataa catcatcgcc
     2581 ttcactgatg ctggtatgcg cataatccat ctggctcacc actttgccat tgattttgat
     2641 tatgagcaat ttgaaagtga agttctttat cttcagcaat tcggccacgg tgaggtggtc
     2701 ggcatcgata acttccttgt tgttattgag ggtgatattc atttgtttag tagttttttc
     2761 ctaaaagtat ttcgagtacc gtgttggcct gcatattggc cgccatacat acgcgcggcg
     2821 ccaggggcgg attgttttca gtagcctcgc tatgcccgtc gccgcataca tagaggtttc
     2881 ccgattgtat catcctgata tcgttgctgt tgccccagcc ggcaatccca acccctgaca
     2941 ctatgggttt gtcgggcatc tggcagagga ccgtttccag tatcattttt ttcatgtcgg
     3001 cccggtcgaa agcttccaca ataacatcgc aaccggcaaa taatttcata ataatttccg
     3061 ggtcaagttt gacaatatga tcctctgcaa tcacttcagg gttaatgttt tgtatgtttt
     3121 ctttcagagc ggtggccttt ttcattccaa gctggttgta aaaaaaatat tgccggttga
     3181 ggttgcctgg ttcaatagcg tcaaaatcgc atagtatcag tttgccaata cctacacggg
     3241 caagggctac cgcacaattg gaacccagcc cgccacaacc cgctattcca acggttttgg
     3301 cgcttaatat tgattttatt ttgttgaaat ccatctgtaa aagggcacaa aaatatataa
     3361 atttgcgaat accggcttat tcaaacacgc tgaaatattt atttccgcta tgactattct
     3421 tcatctttca gcatgcgttt gatatcgttg acggcgccat atatcagcag gatatcccca
     3481 tcatagattt tggtgtcgga agtggccaca tcattcacta tccttttttt gctttctatg
     3541 cccaggatat tgataccgct ttttaccttt atggtggtga gcaccaggat attgtatttt
     3601 cttgtcaggt tcagatccag cagggttttt cccacaaatg attgtggggc aattatttcg
     3661 atgacgttat gatcgctgga gacttcaaag gagtcaacaa aacccggaag gttgagtttt
     3721 ttggctaaac gtatggccgt ttcatgttcg gggtgcagta tttccttgat gcccatggcc
     3781 tccagcacgg caacatgaag ttttgagatg gccctgctga taatcctttt tacattgagt
     3841 tgtttcatca gtgccgtagt cattatcgaa gcgccggtgt cttcgcctat gcttaccacc
     3901 accacatcgg catcgcgcaa aggcaaaact tttacagagt gtatatctgt actgtcgagg
     3961 cacatggcat gagtgacgtc atctttcagt agctgaactt ttaccatatc cttatctaca
     4021 ccaatgactt cgtgtcccat ttgtgtgagt ctcatggcta atgtggatcc gaaatttcca
     4081 agtccgataa caactatttt catggcttta tttgttaatt tatcaaaatg ttttcagtgg
     4141 ggtaatgata atttgtatgc tgtatttttt tgaagaaagc gataagcact gtcagtgttc
     4201 ccaggcggcc aaggaacatg gtgattgaaa ttaccaactt ggaggcttcc gaaaggttcg
     4261 gtgtgaggcc aagagaaagc cctgccgtgc cataagccga gaaacattca aacactacat
     4321 cgaggagttt ttcctccgga tcaaaaaacg acacaaggaa tacggcaagg ccgataacaa
     4381 taaacgaaag catgatgatc gccgaagcac ggcgtaacga tgaatcggct acctggcggt
     4441 ggaacacttc tacccggtct ttgcctttgg ccaggctgat gaaattgagt atgctgatgg
     4501 caaaggtggt ggttttgatg ccgcctccgg tcgacccggg agaggcaccg atccacataa
     4561 gcaacataat caggaatatt actggtacct gaaccacaga aaagtcaata gaattaaacc
     4621 cagcggtccg gtgcgaggag atattaaaaa atgcagtaac cagtttgccg gcccatccat
     4681 ggtcctgcag gctccggttg tattccataa taaaaacaat cagtgtgccg gccaaaatta
     4741 ttatcactgt ggtgacaaca actatacggg tgttgatgtt aatcacccag gccttgtgta
     4801 tatattcgcg gtgtttgtta agacggaata tccggttgac gataaggtgt cttaagtatt
     4861 taagaaaatt aaatataatt ggaaatccca agccgccggc tattatcagt accgacatgg
     4921 taattaaaaa gggatagtta tacaggaaat tcaaatcctg aaaaccatct gatagcgtag
     4981 aaattcctgc attacaaaat cctgaaatgg aatgaaacac cgagaaaaaa atcttctctg
     5041 agagactcat gcctgcattt tcgggcagtt gcgtataaat tatcaatgcc cctgctgctt
     5101 cga
//
LOCUS       DAOSFP010000151         5102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14552_length_5102_cov_4.222905, whole genome shotgun sequence.
ACCESSION   DAOSFP010000151 DAOSFP010000000
VERSION     DAOSFP010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14552_length_5102_cov_4.222905"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..404)
                     /locus_tag="PLI16_05590"
     CDS             complement(45..404)
                     /locus_tag="PLI16_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOH84067.1"
                     /translation="MKELLLSAYLLGISFLLNAAKTDTIVVHSPSMKKNIKACVVIPE
                     NYNNTEKSFPVLYLLHGYGGNYATFIKNFPVISEAADQYGMLLVCADGGLSGWYMDSP
                     VDSLMRYETYITKELVV"
     gene            complement(454..978)
                     /locus_tag="PLI16_05595"
     CDS             complement(454..978)
                     /locus_tag="PLI16_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84068.1"
                     /translation="MSNFHQYSLKLFFVLLSFLFNRNDVTAQHTDSTKISPALQQKWL
                     EQTASRMIDFIPESSTALINPVINDFEEFYMVSFRLIDSGWIKLKNDQWIFLISSSAH
                     SSSEVGDITIAMDNKKHFYTNNGHVCGGIVHFQCRKKIKLKSPSDFFNNFLSDTDGKP
                     WKSLILKNARDGDL"
     gene            complement(1059..2573)
                     /locus_tag="PLI16_05600"
     CDS             complement(1059..2573)
                     /locus_tag="PLI16_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOH84069.1"
                     /translation="MNIQIELILFAVAILFFLSLLAGKAGSRFGVPALLLFLLVGMLA
                     GTDGLGIQFENFQFAQSIGTVALCIILFSGGLDTKLAEIRPVMVRGIVLATVGVFLTA
                     ILSGVIIWWVLGMTIHIACISILSAMLLAAIMSSTDSASVFSILRSRGLSLKNNLRPL
                     LELESGSNDPMAYVLVITFIELISMGSEPNYLQAGLHLVLQLVIGGIVGFALGKFAVF
                     VINRIKIDNISLYPILVFTFCIFIFSSAYLLHGNAYLAVYLAGLVIGNSKFVHKRSAL
                     NFFDGLAWISQLLMFLTLGLLVNPHELIPVLVPGLIISFLLIFISRPLTVFLCLAPFR
                     KMPVKDKTFVSWVGLRGAVPIIFAIFPLAADIPNARFIFNIVFFCTLVSLLIQGTTLA
                     QMAKWLGLAEKPRNIRKPETFDVDFSDEIKSVTCEITIKPEALQKGSHLMDLNLPDQT
                     LVVMIKRGEKYFVPTGKTLLMAGDKLLIITDNQEAMIETYRNLGINNDRSGQNR"
     gene            complement(2643..3311)
                     /locus_tag="PLI16_05605"
     CDS             complement(2643..3311)
                     /locus_tag="PLI16_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOH84070.1"
                     /translation="METKMKALLIGASGLTGGHCLRLLLNDTFYNEVEIWVRKSTGIK
                     HPKLIEKIIDFNVIETFNNTDAQHVFCCLGTTIKNAGNQENFHKIDHDYVIAAATIAA
                     RSGAEKFLYISSIGADSKSKNFYLRTKGKVEDDLKNIFKNGLVIFRPSMLMGKRQEFR
                     FGEVMSKPIMRVFGFLFIGKLKKYKGIEASTVAEAMVRCAKSEVKGLLIMPSDKIAAL
                     AGTA"
     gene            complement(3301..3828)
                     /locus_tag="PLI16_05610"
     CDS             complement(3301..3828)
                     /locus_tag="PLI16_05610"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOH84071.1"
                     /translation="MIIFLTGFMGCGKSSIGLKLAQKISAPFIDMDEQIESRSGKSIT
                     EIFHSQGESVFREMEKEVITQLVKSRDNIVVATGGGTACFFDNMEVMNKNGISVYIKT
                     SPELLFSRLKDDARNRPLLTGQKDLKTFITKKLAEREKYYKKSKYIIEFDQSQTEEEI
                     LEKILKLIKNLPDGN"
     gene            3984..>5102
                     /locus_tag="PLI16_05615"
     CDS             3984..>5102
                     /locus_tag="PLI16_05615"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOH84072.1"
                     /translation="MFKKTNFFFKEKEKKQVFSNFISLSFMQAANYLIPLVAIPYLVR
                     VLGDDRFGLVMFAQAFIQYFVILVDFGFELTATREISIHRNDRKKVTEIFSSVMYVKF
                     VLLLVSFLIMNIVVFSFGQFSKEWQLYYLSFGLVIGQLLFPVWYFQGIEKMKYIMFFN
                     VLAKTIFTVLIFFTIFTPADYRLYAIINSSGFIFAGLVAFFVALYLNNFGFTKPQKNP
                     IKEIILKTRDVFISNVGISLYMTSTSFILGIVTGRNDLVGFYTVAEKTIRGIRYVVSP
                     ITQALYPYLSKRFAGQKTKDSVSVLKKLMLYLTPMLLLLVAGLLIFTEKIVLLLTGSV
                     NPSIILDMRVISVILFIGTYNNVLGVLGMLNMGMEKQFR"
BASE COUNT         1434 a         1035 c         1040 g         1593 t
ORIGIN      
        1 tacctttgtt taaccgtcta taaaaaagtc cgattaatag ggggctagac cactagttct
       61 ttggtaatat aagtttcgta cctcatcaag gaatccacgg gactgtccat ataccacccc
      121 gacagcccgc catcagcgca caccagcagc atgccatact ggtctgccgc ttcggagatc
      181 acaggaaaat tttttataaa agttgcataa tttccaccgt agccgtgcaa tagatacaaa
      241 acaggaaagg atttttctgt attattataa ttttccggta taacgacaca agcttttatg
      301 tttttcttca tggaagggct gtgtactact atggtatctg tttttgctgc gttaagcaaa
      361 aatgaaatcc ctagcagata ggccgataac aacaattctt tcatttttat gaatggtttt
      421 tctttagcaa atcaagtgaa atgaccgtgc tgtttacaaa tctccgtctc ttgcattctt
      481 tagaatcagg gatttccatg gtttgccgtc ggtatcactc aaaaagttat taaaaaaatc
      541 tgaaggagac ttaagtttta tcttttttct gcattggaaa tgcactattc cgccgcaaac
      601 atggccatta ttagtataaa agtgcttttt gttatccatg gcaatcgtga tatcgcccac
      661 ttcagagctg gaatgtgcgg aggaagaaat taaaaatatc cattgatcat tttttagttt
      721 tatccagccg gaatctatta accggaatga aaccatataa aactcttcaa aatcattaat
      781 aacggggttg attaatgccg ttgatgattc agggatgaaa tcaatcatcc gtgatgctgt
      841 ttgttccagc catttttgtt gcagagcagg gctgattttc gtactgtccg tatgttgggc
      901 tgtaacatca ttacgattaa ataaaaatga taatagaacg aaaaatagtt tcagtgaata
      961 ctggtggaaa ttagacatat cctgatttat tttttaatgg tttttattga tcattcggcc
     1021 ttttgcatct ttaatagtgc atgacgatga agtcattttt atctattttg tccacttctg
     1081 tcgttattta ttcccagatt gcggtaggtt tcaatcatag cttcttgatt atcagtgatg
     1141 ataagcaatt tgtcgccggc catcagcaat gtttttcccg taggaacaaa atatttctcc
     1201 ccgcgtttga tcatgaccac cagtgtctgg tcgggcagat tcaaatccat caggtggctt
     1261 cccttttgca gcgcttcagg ttttatggtt atttcgcagg taaccgattt tatttcgtca
     1321 gaaaaatcca catcaaaagt ctccggtttt ctgatgtttc ttggcttttc ggccaggcca
     1381 agccatttgg ccatctgtgc aagtgtggtt ccctgtatta gcaacgacac cagggtgcaa
     1441 aaaaacacaa tgttaaaaat aaaccttgca ttgggtatgt ccgccgccag cgggaaaatg
     1501 gcgaaaataa taggtaccgc gccccgcagc ccaacccacg aaacaaaggt tttgtctttt
     1561 acgggcatct ttcggaaagg ggctaaacaa agaaaaacag tcagcggccg ggaaataaaa
     1621 atcagcagaa aactgataat caggccgggc acaagaacag ggattaattc gtgaggattt
     1681 accaacaagc ccaaagttaa gaacatgagt aactgactga tccaggccag cccgtcgaaa
     1741 aagttcagtg ccgaacgttt atggacaaat ttggagttac ctatcaccag tcctgctaaa
     1801 tacacggcaa gataagcatt tccatggagg aggtaggccg aagaaaaaat gaagatacaa
     1861 aacgtaaaaa ccaaaatcgg atacagggaa atattatcaa tttttatacg gttgataaca
     1921 aacacggcaa attttccaag agcaaaacca acaatacctc cgatgacaag ttgcagcacc
     1981 aggtgtaggc cagcttgcag gtaattgggt tctgagccca tggagatcag ttcaataaat
     2041 gtgatgacca atacgtaggc catggggtcg ttacttccgc tttcgagttc aagcaacggg
     2101 cgaagattat ttttcaggct caggcccctt gaccggagaa tggaaaaaac cgaagccgaa
     2161 tcggtggaag acatgatggc tgccagcagc atggccgata aaatggaaat acaggctata
     2221 tgtattgtca ttcccaatac ccaccagata atcaccccgg agaggatagc cgtaagaaac
     2281 acaccgacag tggccaaaac aattcccctg accataactg ggcgtatttc tgccagtttg
     2341 gtgtccagtc cgcctgaaaa caggatgatg cacaaggcaa cagttccgat tgactgggca
     2401 aactggaaat tttcaaattg tatgcccaga ccgtcggtgc cggcaagcat ccctacaagc
     2461 aaaaagagta acagggccgg aacaccaaat cgagagccgg ctttccccgc caaaagactc
     2521 aggaaaaaaa ggatagcaac agcaaataat attagttcaa tctgaatgtt cattcactgg
     2581 ctttttgcat ttttttcttt gcaataataa cgttttttat cgtgattttc tgtatattaa
     2641 acttatgcgg tgcctgcgag tgcggcgatc ttgtcggaag gcattatcag caagcctttg
     2701 acttctgatt ttgcacaccg taccatagct tcggcaactg ttgatgcctc gataccctta
     2761 tattttttca atttgcctat aaacaaaaaa ccgaaaacac gcattatagg tttgctcatg
     2821 acttccccga aacgaaattc ctgtcttttg cccatcagca tcgagggcct gaaaatgacc
     2881 aggccgtttt tgaaaatatt tttcagatcg tcttcaacct tgcctttagt ccgcagataa
     2941 aagtttttcg atttactatc agcaccgatt gacgaaatgt ataaaaactt ttcggccccg
     3001 gaccgagcgg ctatggtcgc tgcagcaatt acgtagtcgt ggtcaatctt gtgaaaattt
     3061 tcctgatttc ccgcattttt tatggtggtt ccaaggcagc aaaaaacatg ctgtgcatca
     3121 gtattgttaa aggtttctat cacgttgaaa tcaatgatct tctcgatgag tttggggtgt
     3181 ttgattccgg tggatttgcg tacccatatc tccacttcgt tgtagaaggt atcgtttagc
     3241 aaaagccgga ggcaatgtcc gcccgtcagt ccgcttgccc caatcagcaa ggctttcatt
     3301 ttagtttcca tcaggtaaat ttttaattag ttttaggatt ttttccagaa tctcttcttc
     3361 agtttggctc tgatcaaatt cgatgatata ttttgacttc ttgtaatatt tctcacgttc
     3421 agcaagcttt ttagtaataa atgttttcag gtctttttgg cctgtcagta atgggcggtt
     3481 ccgtgcatca tcctttaaac ggctgaaaag caattccggt gaggttttta tataaacgct
     3541 tattccattt ttattcatca cctccatatt atcgaaaaaa caggcagtgc ctccaccggt
     3601 agcaacaacg atgttatcgc gacttttcac caactgggtt atcacttctt tttccatttc
     3661 acggaacaca gattcaccct gagaatggaa tatttcagta atggattttc cggaacggct
     3721 ttctatctgc tcatccatgt caatgaaagg tgcagaaatt ttttgggcaa gtttcagtcc
     3781 gatgctgctt ttcccacaac ccataaagcc agtaagaaaa ataatcatgg tatttttttc
     3841 tgttcaaatg tagcaataat tatttatcag tgaaagctga cctggatact gcagtttatt
     3901 aacattgtgc tgttatcttt tttaagggct gttaaaaatc ctctcatatt taatgactac
     3961 ttttgttttt taccgaaaat ctgatgttta aaaagacaaa tttttttttt aaggaaaagg
     4021 agaaaaaaca agtcttttca aactttattt ctctttcttt catgcaggcg gccaattacc
     4081 ttatcccgct tgtcgctatt ccgtatttgg taagagtttt gggtgatgat cgctttggct
     4141 tggtaatgtt tgcccaggcc tttatacagt attttgtgat attggtggat tttggctttg
     4201 aacttaccgc tacacgtgag atttctattc accgcaacga caggaaaaag gttactgaaa
     4261 ttttcagttc ggtgatgtat gtgaagtttg ttttgttgct ggtgagtttc ctgatcatga
     4321 acatcgtggt tttttcattc ggacagtttt caaaagaatg gcagctttac tatctctcgt
     4381 ttggcctcgt aataggccag ttgctgtttc cagtatggta ttttcaggga attgaaaaga
     4441 tgaaatatat tatgtttttc aatgtgctgg ctaaaaccat atttactgtc cttatatttt
     4501 tcaccatttt tacccctgcc gattatcgct tatacgctat catcaattcc tcgggattta
     4561 tttttgcagg gctggtggct ttttttgtgg ctttgtacct gaacaatttc ggttttacca
     4621 agccgcaaaa aaatcctatc aaagagataa tcctgaaaac aagggatgtg tttatttcca
     4681 acgtcggtat cagcctgtat atgacgtcaa ccagttttat tctgggtata gttaccggcc
     4741 gcaacgacct ggtgggattt tatacggtgg ccgaaaaaac tattcgcgga ataaggtacg
     4801 tggtatcgcc tattacgcag gctttgtacc cttatcttag caagcgtttc gcagggcaga
     4861 agacaaagga ttcagtgagt gtgctgaaaa aactgatgtt atacctgact cctatgttac
     4921 tgctgctggt cgcaggactg cttattttta ctgaaaaaat tgtattgctg ctcacaggat
     4981 cggttaaccc ttccataatc cttgatatgc gtgttatttc ggtaatcctt ttcatcggta
     5041 cttataataa tgtgctcggt gttttaggaa tgttgaatat gggtatggag aaacaattca
     5101 ga
//
LOCUS       DAOSFP010000152         5083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14620_length_5083_cov_3.578560, whole genome shotgun sequence.
ACCESSION   DAOSFP010000152 DAOSFP010000000
VERSION     DAOSFP010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14620_length_5083_cov_3.578560"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="PLI16_05620"
     CDS             complement(<1..1176)
                     /locus_tag="PLI16_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH84073.1"
                     /translation="MQEIRNIAIIAHVDHGKTTLVDRILYQVKLFRENQEKKELILDS
                     NDLERERGITILSKNVSVRYKGVKINIIDTPGHSDFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKALDLGLKPVVVINKVDKPNCSPDEVQEKVFDLMFNLDASEDQLNFP
                     TVYGSSKQGWMSADWRQPTKDVSYLLDVIIRHIPAPRHNPGTLQLQITSLDYSSYVGR
                     IAVGRISRGSIKAGMQLSLVKNDGTITKTFIKELYLFEGLGKEKVKYEVEAGEICAVL
                     GPENFDIGDTIADLENPEGLAPINVDEPTMSMLFTVNNSPFFGKEGVYVTSRHLRERL
                     FRETERNLALRVEETDSPDKLNVFGRGILHLSILVETMRREGYEFQLGQTQVIIKEIN
                     "
     gene            1306..2163
                     /locus_tag="PLI16_05625"
     CDS             1306..2163
                     /locus_tag="PLI16_05625"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOH84074.1"
                     /translation="MAQVTRITIIVLFVVLTGFKMTPYKEKNDVFDGKEIEKSLARVS
                     DSVYIGKFEVSNYLYSLFEKDLKANNNTDLLKIAMVDSANWNDKLAYNKPYADYYYTH
                     PAYKNYPVVNISYDAAILFCQWLTEKYNGFPKRKFKKVVFRLPNSKEWTFAARGNNSS
                     MPYPWGPYLMKDCKYMCKYKHFGDERITYRKDLKIYEIVKNPKGSSPIDIHDVANITE
                     PVNSYYPNDLGLYNVCGNVAEMIIEKGKTRGGSYKNSGWDVRIDAVDTFEKSATDVGF
                     RYLMEILEK"
     gene            2165..2530
                     /locus_tag="PLI16_05630"
     CDS             2165..2530
                     /locus_tag="PLI16_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84075.1"
                     /translation="MITLPLKEKKFVVVADAHSHEPLEIFFADGLPDLHSEIFAVAQK
                     QWAAKGVETVCAGGGRFCVIENFLVFYDRSLKYHRFDSAVVEALSKKHPAFRNKGYRV
                     ISDTRYTNPLELFNQRRTR"
     gene            complement(2527..3828)
                     /locus_tag="PLI16_05635"
     CDS             complement(2527..3828)
                     /locus_tag="PLI16_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84076.1"
                     /translation="MMNIKATKFSFRLAVVLVALFALRLAYGLCSEFWFPDELQVYLI
                     GLKFYATGHIPCFGADVVYTETQIPGALQGLLVGLPFYLFKIPEAPFILLNILSFAVL
                     CFFALYLQKKFPGVPTWFTWIWLMTCPWTMNFSTHVLNPSYVLFGSVLFFIAFFEVIP
                     RISIGFVKPRLAFFLMGFALFWVLQLHMSWVLLIPFICYAFWVSFRRDRMVVIYFFAG
                     CLIVAITLIPTLLAWGLSAFSAGAGNTVFNGSNIKEIGTVLLRFVSFATFEIPRFIDT
                     DGSSKWLFFKDYWPAAPFILLVILAGFLQVAYLIVAFFLKNPQADFAFAKWITLGAML
                     ITWGSFFFSVKGPSSHTFYVLFPLVMIYSFYCWQPLFRRQWFAVIMAVLLISGLITHG
                     TLAYHNYSLKSMYLNRDLPLKAIQEKDYHILGERRAFDKNP"
     gene            4051..>5083
                     /locus_tag="PLI16_05640"
     CDS             4051..>5083
                     /locus_tag="PLI16_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOH84077.1"
                     /translation="MQIAAVYNHLLHKLGLNNKFAGKFMKHKEMNGIKAASIYLLKLF
                     VFWMALFYAFQCLFLAFNFRMLADIPLGHTMYSFLKALPMNVASACYLLLPSLIVLCI
                     GISSKNSTKFFHAARWINWLLIVFYLLISLAGLGLYANWGTKINSKALSFMIYPQEVA
                     GILFDIYNLLYFGILVAITALIFLVYRHFFKTPATRNTGIVRSSIFFVIITGILFVGA
                     RGGVQKYPISKQSCFYSKYPVLNFAALNDFWNFSATLVHPRMKENPYVFYSKSKAEKV
                     ISDLFAIEKDSTEYLLKTSRPNIVLIALESFSADAIACLGGEKGITPCFDSLAKEGFL
                     FTRAYATGFR"
BASE COUNT         1594 a         1120 c         1046 g         1323 t
ORIGIN      
        1 gttgatttct tttatgatta cctgggtctg ccccagctga aactcgtaac cttcgcgcct
       61 catggtctcc accaggatag aaagatgcag tattcccctg ccgaaaacat tgagtttgtc
      121 gggagagtcg gtttcttcca cacgcagagc caggttgcgt tcagtttcac gaaataggcg
      181 ttcgcgcaag tgtctcgaag taacgtacac cccttcctta ccaaaaaacg gcgagttgtt
      241 cacggtgaac aacatactca tggtaggctc gtcaacatta atgggagcca gcccttcggg
      301 gttttccaga tcggcaatgg tatcgccgat atcaaaattt tcggggccta aaacagcaca
      361 tatctccccg gcctccacct catacttgac cttttctttg cccagtcctt caaaaagata
      421 taactctttg ataaaagtct tggtgatagt tccgtcattt ttcaccagcg aaagctgcat
      481 accggctttg atgctgccgc gcgaaatacg tcccaccgcg atacggccca cataagaaga
      541 ataatcaagg ctggtgattt gcaactgcag ggtgcccggg ttatgcctgg gtgcaggaat
      601 atgccttata ataacatcaa gcagatagct tacatccttt gtcggttgac gccaatcagc
      661 cgacatccag ccctgcttgg aagagccata aacggtagga aaattcagtt gatcttcgct
      721 ggcgtccagg ttgaacatga ggtcaaaaac tttttcctgt acttcatcgg gcgaacagtt
      781 gggcttatcc actttattga tgacaaccac gggcttcaat cccaggtcca gcgccttctg
      841 caaaacaaaa cgtgtctgtg gcatcggccc ttcaaaggca tctaccaaca gcagcacccc
      901 gtcggccata ttcagcaccc tttccacctc acctccgaaa tcagagtgcc cgggagtatc
      961 aataatatta attttaacac ctttgtacct gacggaaaca tttttggaaa gtatggtgat
     1021 accccgctcc cgttctagat cgttggaatc aagtatcagt tctttctttt cctgattctc
     1081 gcgaaataac ttcacctgat ataatatacg gtcaaccagg gtcgttttgc catggtcaac
     1141 gtgggctata attgctatat ttctgatttc ctgcatttta aataaacttt ttaccttttt
     1201 tcaaaaagaa gttgcaaagg tagtctttaa atttcacata acaggtcaac aatgaaacat
     1261 ttgattgtac ctttgcaaaa acatacattt aaatattttc ataaaatggc acaggttaca
     1321 agaataacaa taattgttct tttcgttgtt ctcacaggat ttaagatgac accttataaa
     1381 gaaaaaaacg atgttttcga tggaaaagaa attgaaaagt cgctggcacg ggtttccgac
     1441 agtgtgtaca taggcaaatt cgaagttagc aattatttat acagcctttt cgaaaaggat
     1501 ctcaaggcaa ataacaatac cgatctgttg aagatcgcca tggttgattc ggcaaactgg
     1561 aacgacaaac ttgcttacaa caaaccttat gctgactatt attacactca tcccgcctat
     1621 aagaattatc ccgtggtgaa tatttcttat gatgcggcca tacttttttg ccaatggctg
     1681 acagaaaaat ataatggttt tccaaagaga aaattcaaaa aggtggtttt tcgcctgccc
     1741 aacagcaagg aatggacatt tgccgcgagg ggaaacaatt catccatgcc ctatccctgg
     1801 gggccatacc tgatgaaaga ttgtaaatac atgtgcaaat acaagcactt tggtgacgaa
     1861 cgtatcacct atcgaaaaga cctgaaaata tatgaaattg taaaaaaccc aaaaggcagt
     1921 tccccgattg atatacatga cgttgctaac attactgagc ctgtaaattc atactacccc
     1981 aacgatttgg gcctttacaa tgtgtgcgga aatgtggctg agatgatcat tgaaaaaggt
     2041 aaaacaaggg gcggtagtta taagaattcg ggctgggatg tcaggatcga tgctgtggat
     2101 acctttgaaa agtctgccac tgatgtcggg tttcgatact tgatggaaat attggaaaaa
     2161 taaaatgata acactacctc tcaaagaaaa aaaatttgtg gtggttgccg atgcccatag
     2221 ccatgagccg ctggaaatat tttttgccga cggcctgcct gacctgcatt cggaaatttt
     2281 tgccgtggca caaaagcagt gggccgcaaa gggagtagag acagtatgtg caggaggcgg
     2341 caggttttgt gtcatcgaaa acttcctggt gttttatgac agatcactga aataccaccg
     2401 gtttgatagt gctgtggtgg aagccttaag taaaaaacat cctgccttcc ggaataaagg
     2461 ctaccgggtc atcagcgaca ccagatatac caatcctttg gaacttttta accagcggcg
     2521 gacccgttaa ggatttttat caaaggcacg gcgttctccg agaatatggt aatctttttc
     2581 ctgtatagct ttcaaaggca aatccctgtt cagatacatc gatttcaggg aataattatg
     2641 ataggccaat gtaccatggg ttatcaagcc ggaaatcaat aacaccgcca tgataacggc
     2701 aaaccactgg cgacggaata agggctgcca gcagtaaaag gaatagatca tcaccagcgg
     2761 gaataacaca taaaatgtat gtgaagaagg ccctttcaca ctgaagaaaa agcttcccca
     2821 ggtgatgagc atagctccaa gtgttatcca ttttgcaaag gcaaaatccg cttggggatt
     2881 cttaagaaaa aaagccacta tcaggtatgc tacctgtaga aaacccgcca gtattaccaa
     2941 taaaataaaa ggagccgccg gccagtagtc tttaaaaaac agccatttgg agcttccatc
     3001 ggtgtctata aaccggggaa tctcaaaagt ggcaaaagaa acaaacctga gcaacaccgt
     3061 cccgatttct ttgatattgg aaccgttaaa caccgtgttg ccggcgcctg cactaaaagc
     3121 agaaagcccc catgccaaaa gtgtggggat tagcgttatg gcaacaatca gacatccggc
     3181 aaaaaaataa atgaccacca ttctatcacg acggaacgaa acccaaaatg catagcaaat
     3241 aaaagggatt agcaacaccc aggacatatg cagctgcagc acccaaaaca atgcaaaacc
     3301 catcagaaaa aatgcaagcc gtggcttgac aaagccaatg gatatacgag gtattacttc
     3361 aaaaaaagca ataaaaaata ataccgagcc aaaaagcaca taagaaggat taagcacatg
     3421 agtggaaaag ttcattgtcc aggggcaggt catcagccat atccaggtaa accatgtggg
     3481 tacgcccgga aattttttct gcagatacag cgcaaaaaaa caaagcacag caaaagaaag
     3541 aatattcagg aggataaaag gagcctcagg aattttaaac agataaaaag gtaatcccac
     3601 cagcaaaccc tgcaatgcac cgggtatctg tgtttctgta tatactacat cagccccgaa
     3661 acagggtata tggccggtag cataaaactt gaggccaata agataaacct gcagttcgtc
     3721 ggggaaccaa aactcagaac acagcccata agccagtcgc agggcaaaaa gcgcaacaag
     3781 cactaccgcc agacgaaaag agaattttgt tgcttttata ttcatcaccc aaaggtaatt
     3841 attatcatat cacacaaaca aaacgtaaac aaaattcagg attatttacc cactgcttta
     3901 aaaaccaaaa ttcatttttg gatagcacaa gaaaaagtga aaatattttt catacacgca
     3961 aatcaatacc ttccattgga tacatcctgc gtccatgggt acgacataaa gatttgaaca
     4021 ggatgtgaac agtcgctcaa aaaaaaacat atgcagatag ccgcagttta caaccacttg
     4081 ttgcacaagt taggattaaa taataaattt gcagggaaat ttatgaaaca caaagaaatg
     4141 aacggtataa aagcagcatc catctatttg ctgaaactct ttgtgttctg gatggcattg
     4201 ttttacgcct tccagtgttt atttctggcg tttaatttcc gcatgctagc ggatattcca
     4261 ttggggcata ccatgtattc gtttttgaaa gcgctgccaa tgaatgttgc ctcggcgtgt
     4321 tatttattat taccttctct cattgtttta tgtattggaa tttcatcaaa aaacagtaca
     4381 aaattttttc atgcggcaag gtggattaat tggttgctaa ttgtttttta cctgctcata
     4441 agccttgccg gtcttggact ttatgccaac tggggaacta aaataaactc gaaagccttg
     4501 tcgtttatga tctatccgca agaagtggcc ggcatattat tcgacattta caacctgctg
     4561 tatttcggga ttctggtagc catcacagca ttgatatttc ttgtctaccg gcattttttc
     4621 aaaacacctg caaccagaaa cacagggata gtgcggtctt cgattttttt tgtaatcatt
     4681 accgggatac tttttgtggg ggcccgcgga ggtgttcaaa aatacccaat cagcaaacaa
     4741 tcctgtttct attctaaata tcctgtgcta aacttcgcag cactgaacga cttctggaac
     4801 ttttcggcca ccctggttca tcccaggatg aaagaaaatc cttatgtatt ctacagtaaa
     4861 agcaaagcgg aaaaggtaat ttcagacctc ttcgcaatag aaaaagacag cacagaatac
     4921 ctgctgaaaa cctccagacc gaatattgtt cttatagctc tcgaaagttt cagcgccgac
     4981 gctattgcct gcctgggagg ggaaaaaggc attaccccct gttttgattc actggcaaaa
     5041 gagggattct tatttacccg ggcctatgcc accggtttcc gca
//
LOCUS       DAOSFP010000153         5076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14652_length_5076_cov_4.070504, whole genome shotgun sequence.
ACCESSION   DAOSFP010000153 DAOSFP010000000
VERSION     DAOSFP010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14652_length_5076_cov_4.070504"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /gene="rplM"
                     /locus_tag="PLI16_05645"
     CDS             <1..386
                     /gene="rplM"
                     /locus_tag="PLI16_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOH84078.1"
                     /translation="AEKEIVGRLASKVAILLRGKNKTYYTPNLNCGDNVIIINAEKVQ
                     FTGKKLTDKMYVRHTGYPGGQRLTTPRQYLAKKPEKILELAIRGMLPKNKLGDDMYKH
                     LFVYAGSEHPHEAQKPNKININTIK"
     gene            390..776
                     /gene="rpsI"
                     /locus_tag="PLI16_05650"
     CDS             390..776
                     /gene="rpsI"
                     /locus_tag="PLI16_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014708903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOH84079.1"
                     /translation="MEVIHTIGRRKTAVARIYVKEGKGEIVVNSKDFKEYFPTPTLQY
                     VVKQPLELTNSSEKYDIKANIDGGGFNGQAEALSLAISRALCEIDAEHRPVLKPKMLL
                     RRDPRMVERKKPGQKKARKRFQFSKR"
     gene            889..1842
                     /gene="rpsB"
                     /locus_tag="PLI16_05655"
     CDS             889..1842
                     /gene="rpsB"
                     /locus_tag="PLI16_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOH84080.1"
                     /translation="MNQLKFEDLLDAGVHFGHLKRKWNPAMAPYIFMERNGIHIIDLY
                     KTVAKADEAALALKQIAKSGKKILFVATKKQAKEIVGELVRSVNMPYVTERWPGGMLT
                     NFATIRKAVRKMASIDKMKNDDTFVKISKRERLQIIRERAKLEKNLGSIADLTRLPSA
                     LFIVDILKEKIAVNEAKKLNIPTFAIVDTNSNPNEVDYAIPANDDASKSIAIILGHMV
                     KAIEEGLAERKIAKDVQDREDEEDNEDILIEKASLKSIEEQEEEEEAKSLKKAAAKKE
                     LSAKIKDTDIAGETEEDEDKKARKPVAVKKPVAGKKTITKK"
     gene            1871..2707
                     /gene="tsf"
                     /locus_tag="PLI16_05660"
     CDS             1871..2707
                     /gene="tsf"
                     /locus_tag="PLI16_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOH84081.1"
                     /translation="MANITAAEVNKLRQMTGAGMMDCKHALVECDGDYEKAIDYLRKK
                     GQKVANKRADRETSEGLVIALATEDKKFGTITMLNCETDFVAKNQEFIDLANAIASLT
                     LTNRPKSLEELKNLTLNNRSVAEHVSDYMGKTGEKIGLNEYQFIEAPRVFVYNHMGNK
                     LASMLGFSKSEGKDIDQCGKDVAMQIAAMSPVAIDKDSVDQTTIDREIEIGKEQARAE
                     GKPEDMLEKIAIGKLNKFFKDNTLVNQEFIKDSKKTVGQYLSECDKDLKITEFKRVSV
                     KN"
     gene            2788..3495
                     /gene="pyrH"
                     /locus_tag="PLI16_05665"
     CDS             2788..3495
                     /gene="pyrH"
                     /locus_tag="PLI16_05665"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOH84082.1"
                     /translation="MKYKRILLKLSGEALMGNKEYGFDSDRIDLYANEIKKIYDLHIQ
                     IAIVIGGGNIFRGLKGNTFGIERVQGDYMGMLATVINGLALQAALENKDIKCKILSGV
                     QVNPVCELTSGRKAIQHLEKGEIVIISGGTGNPFFTTDTAAVLRALEIKADIILKGTR
                     VDGIYTDDPEKNPEAKKFNTLTFDEAYDKNLKIMDMTAFTLCKDNNMPVVVFNMNEEG
                     NLQKIVTGGTIGTLVTN"
     gene            3529..4092
                     /gene="frr"
                     /locus_tag="PLI16_05670"
     CDS             3529..4092
                     /gene="frr"
                     /locus_tag="PLI16_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOH84083.1"
                     /translation="MSEELDFLIEETNESMQNALVHLEREFQKIRTGKASTQMLEGIK
                     IEYYGAVTPLEQLSNINTPDPKQIIIQPWDKNTLPLIEKAIMAANLGFNPKNDGEVLR
                     IVVPPLTEERRKELVKKARIEAENAKVSIRNIRRNSNDAAKKLEKEGVPEDLVKKAQN
                     DIQEMTDNFIIKTDKLLAQKEKDIMTV"
     gene            complement(4109..>5076)
                     /locus_tag="PLI16_05675"
     CDS             complement(4109..>5076)
                     /locus_tag="PLI16_05675"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH84084.1"
                     /translation="TRTKTIMRTILITGGAGFIGSCLAEKMISDPENFVVIVDDLTTG
                     DVSKLPSAQHKNWRFIKCNVNDYRDISAVMLSFPFDYVFHYAAVVGVQRTQLFPVKVL
                     NDIKGISNILNLSKNSHVKRVYFSSSSEVYGEPVELPQNELTTPLNSRVPYAVVKNIG
                     EAYLKSYHKEYGLDYTIFRFFNTYGPKQSRDFVMSRFMALALKNQNITIYGDGSQSRT
                     FCYINDNVDACYASFVNSAVKNVTVNDIINIGNDNEISILDLANKIISLTNSQSKIVY
                     KPPLEEGDMHRRLPDTTKMRKLLGRPMLSIDEGIKKLIENPLFLK"
BASE COUNT         1810 a         1121 c         1018 g         1127 t
ORIGIN      
        1 atgcagaaaa agaaattgta ggaagactgg caagcaaagt ggctattttg ctcagaggca
       61 aaaacaaaac ttattacact cccaacctca actgcggaga taatgtaata atcatcaatg
      121 ctgaaaaagt tcagtttacc ggcaaaaaac tcaccgacaa gatgtatgta agacataccg
      181 gataccccgg cggccagcgt ctgaccacgc cccggcaata tcttgccaaa aaaccggaaa
      241 aaattcttga actggctatc agaggcatgc tgccaaaaaa caaactggga gacgatatgt
      301 acaaacacct ttttgtttat gcaggctccg aacacccgca cgaagcacaa aaaccgaata
      361 aaattaatat taataccatt aaataattta tggaagtaat tcacaccatc ggaagaagaa
      421 aaacagccgt tgcccgcatt tatgtaaaag aaggtaaagg cgagattgtt gttaacagca
      481 aagattttaa agaatacttt ccaacaccca cattgcaata tgtggtaaaa cagcccttgg
      541 aactgacaaa ttcttcagaa aaatatgata taaaagccaa tatcgacggc ggcggcttca
      601 acggacaagc tgaagcatta tccctggcca tttcacgcgc tttatgcgag attgacgcag
      661 aacaccgccc ggtattaaaa cccaaaatgc tcctcagaag agacccgaga atggttgaac
      721 gtaagaaacc cggacagaaa aaagcaagaa aaagattcca gttcagcaaa cgttaacatt
      781 ccgttttgtt tagtatctaa attgccaaga ctatcgtttt gcgatactac ttggcaattg
      841 acttaaaaaa agtaaacaag aaagtaaaca taacctaaat taaaattcat gaatcaatta
      901 aaatttgaag acctcctcga tgccggagta catttcggac atctgaaaag aaaatggaat
      961 cccgccatgg caccatacat ttttatggaa cgcaacggga tacacattat cgacctttac
     1021 aaaaccgtag ccaaggccga tgaagccgca ttagctctca aacagatcgc caaatcaggc
     1081 aaaaaaatcc tgtttgttgc caccaaaaaa caagctaaag aaatcgtcgg cgaactcgtg
     1141 agaagcgtga atatgcctta cgtaactgaa cgctggccgg gcggtatgct taccaacttt
     1201 gccaccatcc gcaaagctgt aaggaaaatg gcctccatcg acaaaatgaa aaacgatgac
     1261 actttcgtga aaatctcaaa acgcgaacgc ctgcagatca tccgcgaacg tgccaaactc
     1321 gaaaagaact taggtagcat cgccgacctt acccgcctgc cttctgcctt attcatcgtg
     1381 gacatcctga aagaaaaaat cgcagtcaac gaagccaaaa aactgaatat ccccaccttc
     1441 gccattgtgg ataccaattc caatcccaac gaagttgatt acgctattcc cgccaacgat
     1501 gacgcttcaa aatctatagc tatcattctt ggacacatgg tgaaagctat cgaagaaggc
     1561 cttgccgaac gcaaaatagc taaagatgtt caggacagag aagacgaaga agataacgaa
     1621 gatatcctga tcgaaaaagc cagcctcaaa tcaattgaag aacaagaaga ggaagaagaa
     1681 gccaaatctt tgaaaaaagc tgcagcaaaa aaagaactgt cagcaaaaat caaagatact
     1741 gacatcgccg gagaaaccga ggaagacgaa gataaaaaag cacgcaaacc tgttgcagta
     1801 aaaaaacctg tcgcaggcaa aaaaaccata actaaaaaat aaacattaac ctaattatcc
     1861 caatactaaa atggcaaata taactgcagc cgaagtaaac aaattaaggc aaatgaccgg
     1921 cgccggcatg atggattgta aacacgccct ggtagaatgc gacggagatt acgaaaaagc
     1981 tattgattac ctgcgcaaaa aaggacaaaa agtagccaac aaacgcgccg accgcgaaac
     2041 ttccgaaggc ctggttatag ccctggctac cgaagacaaa aagtttggaa ccatcaccat
     2101 gttgaactgc gaaaccgact ttgtcgccaa aaaccaggaa tttatcgacc tcgccaacgc
     2161 catagcttcc cttaccctga ctaacagacc caaatcgttg gaagaactga aaaacctgac
     2221 tctcaacaac cgcagcgtag cagaacatgt gagcgactat atgggaaaaa caggcgaaaa
     2281 aatcggactc aatgaatacc agtttatcga agctcccaga gtgttcgtat ataatcatat
     2341 gggcaacaaa ttagcttcta tgctgggctt cagcaaatcc gaaggtaaag acattgacca
     2401 gtgcggaaaa gatgtcgcca tgcagattgc cgctatgagc cctgtagcta ttgacaagga
     2461 cagcgtggat cagacaacga tagacagaga aatagaaatc ggaaaagaac aggcccgcgc
     2521 cgaaggaaaa cctgaagata tgctcgaaaa aatcgccatc ggaaaactca ataaattctt
     2581 taaagacaac acccttgtca accaggaatt tattaaagac agcaaaaaaa ccgtgggaca
     2641 atacctttcc gaatgtgaca aagacttgaa aattactgaa tttaaaagag tttcagtgaa
     2701 aaactaatct tttatcacac aaataaaaaa aagagaggcc gcaggttctc tctttttttt
     2761 tatttttgcc ctaataaaaa ttttctcatg aaatataaaa gaattctgct gaaactgagt
     2821 ggtgaagccc tgatgggcaa taaagaatat gggtttgact ccgatcgcat tgacttgtat
     2881 gccaatgaaa taaaaaaaat ctacgacctc catattcaga tcgccattgt gattggcgga
     2941 ggcaatattt tcagaggcct gaaaggcaac acctttggca ttgaacgggt gcagggagat
     3001 tatatgggca tgctggctac ggttatcaac ggcctggcgc ttcaggcagc cctggaaaac
     3061 aaagacataa aatgcaaaat actctcgggt gtacaggtaa atcccgtatg tgaactgacc
     3121 agcggaagaa aagccataca gcatcttgaa aaaggagaaa tcgtgattat ctcgggaggt
     3181 accggcaatc ccttttttac aacagatacc gccgcagtgc tgcgggcatt ggaaatcaag
     3241 gccgacatca ttctgaaagg tacccgcgtt gatggcatct ataccgacga ccccgaaaaa
     3301 aatcccgaag cgaaaaaatt taacaccctt acctttgacg aggcctacga taaaaatttg
     3361 aaaatcatgg acatgacggc atttacccta tgcaaagata acaatatgcc cgtagtggtt
     3421 tttaatatga acgaggaagg gaacctgcaa aaaatagtta caggaggaac gataggcacg
     3481 ctggtaacaa attaattttc aaaacagtca ataataaaaa aaacaaatat gtctgaagaa
     3541 ttagattttc tgatagaaga aaccaacgaa agtatgcaaa atgcactggt acacctggaa
     3601 cgcgaatttc aaaaaatccg tacaggaaaa gccagcacac aaatgctcga agggataaaa
     3661 attgaatatt acggcgccgt gacgcccctg gagcagctat ccaacatcaa tacccctgat
     3721 cctaaacaaa ttattattca gccatgggac aaaaacaccc tgcccctgat agaaaaagcc
     3781 atcatggccg ccaatcttgg attcaacccc aaaaatgacg gggaagtgtt gcgtattgtg
     3841 gtgccacctc ttactgaaga acgccgcaaa gaactcgtta aaaaagcccg catcgaagcg
     3901 gaaaatgcca aggtttccat ccggaacatc cgcagaaatt caaatgatgc agcaaaaaaa
     3961 cttgaaaaag aaggtgtgcc cgaagacttg gtgaaaaaag cccagaacga catacaggaa
     4021 atgaccgaca atttcattat aaaaacagac aaattgctgg ctcaaaaaga aaaagacatc
     4081 atgacggtat aatacaccgc ctcaggattt atttaagaaa aagcggattt tctattaatt
     4141 tttttattcc ttcatcaata gaaagcattg gccggcccag caactttcgc atcttggtgg
     4201 tatcgggcag acgccggtgc atatcaccct cttccaaagg cggtttataa actatcttcg
     4261 actgggaatt ggtcagactt atgattttat ttgccaggtc aaggatagaa atttcattat
     4321 cgttgccaat gttgataata tcgttgaccg taacattttt aacagccgaa tttacaaaac
     4381 tggcatagca ggcatccaca ttgtcgttga tataacaaaa agttctcgac tggctgccgt
     4441 cgccatatat ggtgatattc tgatttttca acgccagcgc cataaagcgc gacattacaa
     4501 aatcacggct ttgcttgggc ccataggtat tgaaaaacct gaaaatggta tagtccaggc
     4561 catactcttt atgataagat ttcagatagg cttccccgat gtttttaacc acagcataag
     4621 gtacccgcga gtttaaaggc gtggtcagct cgttttgcgg caactctacc ggctcgccgt
     4681 aaacctccga agaagaagaa aaataaaccc gtttgacatg agagttctta ctcagattta
     4741 aaatgttgga gatacctttt atatcattca aaaccttcac aggaaacaac tgcgtacgct
     4801 gtaccccaac cacggcggca taatgaaaaa cataatcgaa aggaaaagac agcatcacgg
     4861 cagaaatatc acggtaatcg ttgacattgc atttgataaa acgccagttt ttatgctgag
     4921 ccgatggcag tttagacacg tcgcccgttg tgaggtcgtc cacaataaca acaaaatttt
     4981 cggggtcgct gatcattttt tcggccagac agcttccgat aaatcctgcc ccgccggtaa
     5041 tcaatattgt tctcataatt gtttttgttc tagtat
//
LOCUS       DAOSFP010000154         5070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14676_length_5070_cov_5.013759, whole genome shotgun sequence.
ACCESSION   DAOSFP010000154 DAOSFP010000000
VERSION     DAOSFP010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14676_length_5070_cov_5.013759"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLI16_05680"
     CDS             <1..844
                     /locus_tag="PLI16_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOH84085.1"
                     /translation="WQVDESLSQSGENAVSITWFNHRFNQALEELNDHYEKYRMSDAL
                     MTVYKLIWDDFCSWYLEMIKPAYQKPIDKATIDATIDIFEKLMQVLHPFMPFITEELW
                     QHLRDRKEGESIMISSMPEAQSFEISFIEKFAYVQDVISQVRMIRKEKNIPNKEAIIL
                     KIRKNNNEQPDTTFDGIASKLCNISEIEYVSSKPDNALTFLVKSTEFYIPVGSSINVE
                     SELKKLNEELKYYRGFLESVNKKLSNDRFVNSAPQQIVDAEKKKKEDAENKIRIIEEQ
                     IRSL"
     gene            922..1341
                     /locus_tag="PLI16_05685"
     CDS             922..1341
                     /locus_tag="PLI16_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HOH84086.1"
                     /translation="MSLSIFDNKSQVPTEEMLAQTLQDSCLWWNQLRAFVIKHYPSAM
                     EEWKHSGKNYGWGFRLKDKKRAIIYLTPADGYFLFSVVFGEKATQEALQSTISDELKE
                     IISTAKVYAEGRGIRIEVKSANLIGDLQQMILIKLKN"
     gene            1476..3935
                     /gene="pheT"
                     /locus_tag="PLI16_05690"
     CDS             1476..3935
                     /gene="pheT"
                     /locus_tag="PLI16_05690"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOH84087.1"
                     /translation="MKISYNWLKQYTGLNIDPEELSLILTNCGLEVEAMEHAGIDKSR
                     LEGVVVGYVAEKVKHPNADKLSLTKVDVGTGQLLSIVCGAPNVAQGQKVLVATIGTKM
                     TTPKGEFTIQKTKLRGEISEGMICAEDELGLGESHAGIMILDEDTPVGMAAKDYLNTE
                     EDFTYEIGLTPNRSDATSHIGVARDVIAVINCQNNSEKKAIIPDISSFRPDNNSLPIN
                     VIIEDTKACPRYTGLTITGVQVADSPAWLQEKLKTIGLRPINNIVDITNYVLFETGQP
                     LHAFDASCISGNTVIIKKLPQGTKFKTLDGVERELSADDLMICNREEGMCIAGVFGGE
                     KSGVTSQTKNVFLESAYFNPVSVRKTSKLHGVKTDASFRFERGADPNITVFAIKRAAL
                     LIKEIAGGEISSEVVDVYPEKIADRSVDFSLTKLNLLAGKDIDKNIVTNILNSLEIEI
                     AATSGDVLTLKVPPFRTDVTREADIIEEILRIYGYNNIECSERVHASLSYAPNPDAEK
                     IQHVISDYLTANGFYEMMTNSLTKQAYYTNNSNIFSPEHLVNLVNPLSKELNVMRQTM
                     LFNGLEAIAFNKNHKNNDTLFYEFGRVYFYHKSSTENPLEKYNEKKQLALFASGNIVK
                     PSWHSKEQPADLYFIKAMAMNVMRRMGIDINQLKSSENATPELFSSQITYYHNGEPVC
                     DLGKINPAIVTQLDIKDEVFFALLEWEKLIFDLPKQEVKYVEVSRFPEVRRDLALLID
                     STVKYAEIERIAFETEPALIKKVELFDIYEGENIGTNKKSYAVSFILQDEEKTLTENR
                     IEDTMNRLIDAYKNKLNAILR"
     gene            3940..4710
                     /locus_tag="PLI16_05695"
     CDS             3940..4710
                     /locus_tag="PLI16_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH84088.1"
                     /translation="MSFLFESIVFGPVKSRRLGVSLGMNIVPAHIKFCSFNCIYCECG
                     WTQASEAETANFYSPELIREALENRLQSLTAEGIAPDAITFAGNGEPTIHPQFDVIVD
                     DTIALRDKYFPKARVVVLSNSSTSGNPIVRAALLKTHNIMKLDAGTEETFQLINLPRT
                     KISLAQIVENLKKFNGNLIIQIMFIRGTVGNKTIDNTRPAELNPLLKYLTEINPTEVM
                     IYSLDRLPPMTQLEKISKEELEIIAGKIRQLNYPCTVY"
     gene            4714..4953
                     /locus_tag="PLI16_05700"
     CDS             4714..4953
                     /locus_tag="PLI16_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84089.1"
                     /translation="MWLQNKGYLIKTFEFNNLASAADFAEAIAEPRILTFIKASLAEK
                     KHFVTVKIKSRHHADTLSKKEKKILDGIEEKYFHM"
BASE COUNT         1642 a         1163 c         1049 g         1216 t
ORIGIN      
        1 atggcaggtt gacgaatcct taagccaatc cggcgaaaat gccgtttcca taacatggtt
       61 taaccaccgc tttaaccagg cgcttgaaga gctgaatgat cattacgaaa aataccgcat
      121 gtcggatgcc ctgatgactg tgtataagct gatatgggac gatttctgct cgtggtatct
      181 cgagatgata aaacccgcct accaaaaacc tatcgacaaa gcaaccattg acgccaccat
      241 cgacatcttt gaaaaactca tgcaggtatt acatcctttt atgcctttta ttaccgaaga
      301 gctctggcag cacctgcgcg acagaaaaga aggcgagtca attatgatat cgtccatgcc
      361 ggaagcccaa tcttttgaaa tcagctttat tgagaagttt gcttatgttc aggatgttat
      421 ttcccaggtt cgtatgatcc gcaaagaaaa aaacattccg aataaagaag ccatcatact
      481 gaaaatacgt aaaaacaaca acgaacaacc cgacaccacc tttgacggga ttgcctcaaa
      541 gctgtgcaat atcagcgaga ttgaatacgt aagctcgaag cccgataacg cactcacttt
      601 cctggtaaaa tctaccgagt tttatatacc tgtcggaagc agcataaatg tggaaagcga
      661 attaaaaaaa cttaatgaag agctgaagta ttaccggggt tttctggaat ctgtaaataa
      721 aaaactcagc aacgaccgct ttgttaatag tgcaccccaa caaattgttg atgccgaaaa
      781 gaaaaaaaag gaagatgcgg aaaacaaaat acgcattatc gaagagcaaa tccgttcgct
      841 gtagcatcgg acatttagcc acgggagcga cattgcacgc gaagcattgt acactatgca
      901 tatgaaataa ctatcatagc catgtcacta agcatattcg acaacaaatc acaggtacca
      961 acggaagaga tgcttgctca gacattgcag gactcctgtt tatggtggaa tcaactcagg
     1021 gctttcgtaa taaagcatta cccgtcggcg atggaagaat ggaaacattc gggcaagaat
     1081 tacggctggg gattcaggtt aaaggataaa aaacgcgcca ttatctacct gactcctgct
     1141 gacggttatt ttcttttttc cgttgttttt ggtgaaaaag ccacgcagga agcgctgcaa
     1201 agcactattt cggatgagtt gaaggaaatt atcagcaccg ctaaggtata tgctgaagga
     1261 agaggtatcc gcattgaggt aaaatcagca aatctcatag gcgaccttca acaaatgatt
     1321 ctgatcaaac tcaaaaacta agctgcataa aaagaacgtt tgttatttca aaaaaagatt
     1381 tccaaagcat gtggaagtcc tccagaaaaa ttatcctctt atttaatgta ccataatctc
     1441 atctgcattg tttatttttg caaaaaaaag caaaaatgaa aatttcctat aactggctga
     1501 aacaatatac cggcctgaat attgaccccg aagaactttc gttaatcctt accaactgcg
     1561 ggcttgaagt ggaagcaatg gaacatgcag gcatcgacaa aagccgtctg gaaggtgtgg
     1621 tggtgggcta tgttgccgaa aaggtcaaac accccaatgc cgacaaactc agtttaacca
     1681 aagtggatgt gggtaccggc caactgttgt cgatagtgtg tggtgcaccc aatgtagcac
     1741 agggacaaaa ggtgcttgtg gcaaccatag gcaccaaaat gacaacaccc aaaggcgaat
     1801 ttacaattca gaaaaccaaa ttacgcggcg agatatcaga agggatgatt tgcgccgaag
     1861 atgaactcgg gctgggcgaa tcacatgccg gcattatgat acttgacgag gacacccctg
     1921 ttggcatggc cgctaaagac tatctgaaca cagaagaaga ttttacttac gaaatcggcc
     1981 tcacccccaa ccgctccgat gccacctcgc atataggtgt tgcccgcgat gttattgccg
     2041 taataaactg ccaaaacaat tcagaaaaaa aagctatcat ccccgacatc agcagtttca
     2101 ggccagacaa caactcgttg ccaataaatg taattatcga agacaccaaa gcctgtccgc
     2161 gctacacagg ccttaccatc acaggggttc aggtagccga ttcgccggcc tggctgcagg
     2221 aaaaactgaa aaccatcggc ctgagaccaa tcaacaatat tgttgacatc accaactatg
     2281 tgctgttcga aacaggacaa cccctccatg cttttgatgc atcctgcatt tcaggaaaca
     2341 ctgttataat aaaaaaactg ccacaaggca ccaaattcaa aaccctcgat ggtgtcgaaa
     2401 gggaattgtc tgctgacgac ctgatgattt gcaaccgcga agaaggtatg tgtattgccg
     2461 gtgtctttgg cggcgaaaaa tcaggggtaa caagccagac aaaaaatgtt tttctcgaaa
     2521 gcgcttattt caacccggtt tcggtaagga aaacctcgaa actgcacggg gtgaaaacag
     2581 atgcctcatt ccgttttgaa cgcggggccg accctaacat cactgtattc gccatcaaac
     2641 gcgctgccct gcttatcaaa gaaattgccg gaggcgaaat ctcttcggaa gttgtggatg
     2701 tgtatcccga aaaaatcgcc gaccgtagtg ttgatttttc gcttacaaaa ctcaatcttc
     2761 tggcaggcaa agacattgac aaaaatattg ttaccaatat tcttaattcg cttgaaattg
     2821 aaattgcagc cacctcgggc gatgtgctta ccctgaaggt gccgccgttc cgtaccgatg
     2881 ttacgcgcga agcagatatc attgaagaaa ttctcagaat ttatggatat aacaatatcg
     2941 aatgcagcga acgggtgcat gcatccttaa gctatgcccc caaccctgat gcggaaaaaa
     3001 ttcagcatgt catttcggac tacctcaccg cgaacggttt ttatgaaatg atgaccaact
     3061 cactgacaaa acaggcctac tataccaaca acagcaacat tttttcgccc gaacatcttg
     3121 tcaacctggt aaaccctctc agcaaagagc tcaatgtgat gcgccagacc atgttgttca
     3181 acgggctgga agctattgcc ttcaacaaaa accataaaaa caacgacaca ttattttacg
     3241 aattcggaag ggtatatttt taccacaaaa gcagcacaga aaacccactc gaaaaatata
     3301 acgaaaaaaa acaattggcg ctgttcgcca gcggtaatat tgtaaaaccc agctggcaca
     3361 gcaaagaaca gcctgccgac ctgtatttta tcaaagccat ggccatgaat gtcatgcgaa
     3421 ggatgggcat tgatataaac caactaaaat ccagtgaaaa cgccacgccg gaattgttct
     3481 cctcacagat aacctattat cacaacggag aacccgtttg tgatctgggc aaaatcaatc
     3541 cggccatagt aacccaattg gatattaaag acgaggtctt ttttgccctg ttggagtggg
     3601 aaaagctaat ctttgacctc ccaaaacaag aggtaaaata tgtggaagta agccgttttc
     3661 ctgaagtccg ccgcgacctg gccctgttga ttgacagtac ggtgaaatat gcggaaattg
     3721 aacgcattgc cttcgaaacc gaacctgccc tgataaaaaa ggttgaactc tttgacatct
     3781 atgaagggga aaacataggg acaaataaaa aatcgtatgc ggtgagtttt atccttcagg
     3841 acgaagaaaa aaccctgacc gaaaaccgga ttgaggacac catgaacaga ctcattgatg
     3901 cctataagaa taaacttaat gctatactga gataagacca tgagtttttt atttgaatca
     3961 atagtttttg gaccggtgaa aagccgccgg ctgggtgttt cccttggtat gaacattgtt
     4021 ccggcacaca taaaattttg ttcattcaac tgcatctact gcgaatgcgg atggacacaa
     4081 gccagcgaag cagaaactgc aaatttttat tctccggaat taatccgcga agcgctcgaa
     4141 aaccggcttc aatcgttgac agccgaaggc attgctcctg atgccattac ctttgccggc
     4201 aatggcgagc ctaccataca ccctcagttt gatgtcattg ttgatgatac catcgctttg
     4261 cgcgacaaat attttccaaa agcccgggtt gttgtgcttt ccaactcctc cacctcgggc
     4321 aacccaatag ttcgtgccgc cttactgaaa acgcacaaca ttatgaaact ggatgctggc
     4381 accgaagaaa cttttcaact gataaacctg cccagaacca aaatttccct ggcccaaatt
     4441 gttgaaaatt taaaaaaatt caacggcaac ctcatcattc agattatgtt tatccgtggc
     4501 actgtgggca acaagaccat tgacaatacc cggcccgctg aactcaaccc ccttttaaaa
     4561 tatcttaccg aaataaaccc cacggaagtc atgatttatt cgctcgaccg cctgccacca
     4621 atgacacaac tggaaaaaat cagcaaagaa gaattggaaa tcattgccgg aaaaatcagg
     4681 caattaaact atccatgcac cgtttattaa attatgtggt tacaaaacaa agggtatctt
     4741 ataaagactt ttgaattcaa caatctggcc agcgctgccg attttgcgga ggccattgca
     4801 gaaccccgca ttttaacgtt cattaaagca tctctggcag aaaaaaaaca tttcgttact
     4861 gtaaaaatca agagcaggca ccatgctgat acactttcca aaaaagaaaa aaaaatactc
     4921 gacgggatag aagaaaaata ttttcacatg taatcaggat atgctaaact atgaaccaaa
     4981 aatattgctt gcttttggtg aggctgtaac cggaaacaac cagatttttc gctggctggc
     5041 cgataacggg tacctggagc tggcttcgct
//
LOCUS       DAOSFP010000155         5053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14751_length_5053_cov_3.076230, whole genome shotgun sequence.
ACCESSION   DAOSFP010000155 DAOSFP010000000
VERSION     DAOSFP010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14751_length_5053_cov_3.076230"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="hypD"
                     /locus_tag="PLI16_05705"
     CDS             complement(<1..663)
                     /gene="hypD"
                     /locus_tag="PLI16_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOH84090.1"
                     /translation="MKYIDEFRNAELVKILTEKIRAEAKKEINLMEVCGGHTWAIQKF
                     GIPSLMPKEVRLISGPGCPVCVTGRRYIDHAIAYSRLNDTIITTFGDLIRVPGSTSSL
                     NLEKSKGADIRIVYSILDALKIARVNPSKKVIFLGIGFEATASSSAAGVFNAKKENLT
                     NFFLFSSHKIMPPAMSALIDEGIKINGYLAPGHVSAITGSHIYDEFPVKYKVGCVISG
                     FEP"
     gene            complement(684..935)
                     /locus_tag="PLI16_05710"
     CDS             complement(684..935)
                     /locus_tag="PLI16_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOH84091.1"
                     /translation="MCLGIPSKIVSIDGDNAIVSVGGTRVEASLQLLTDIQVGDYVLL
                     HTGFAIEKISPEEAEETLKLLQKLGEPEGEIKPSENKVD"
     gene            complement(1032..3281)
                     /gene="hypF"
                     /locus_tag="PLI16_05715"
     CDS             complement(1032..3281)
                     /gene="hypF"
                     /locus_tag="PLI16_05715"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOH84092.1"
                     /translation="MNTYLIEIKGLVQGVGFRPFIYRIAAGFHLNGQVENRNDGVFIV
                     VNCDEKTVKSFIQAIWEQAPQAAAIESVDFRITDTKFFDDFQIVKSRTVSNRITDISP
                     DIAVCEDCLRDMKTRQNRIDYTFINCTNCGPRFTLIQNIPYDRAKTTMSPFVMCDDCK
                     KEYLDVIDRRFHAQPVACSVCGPEYTLFEGKKELKGIENIVSRVQYLLQNKKIVAIKG
                     IGGFFIACNAVCEETVARLRKMKNREGKPFAVMFSGMETLKEYAFVNAQEEKSLVSYR
                     RPIVLLYQKKSLAPSVNVGFDTIGCMLPYMPFHYLLFRGLTIPAIVLTSGNISDEPII
                     TDNEIAIEKLSDITDAILSYNRAIYNRTDDSVVTIVNKHERLIRRSRGYVPTPLKLTT
                     DVDGILATGAELVNCFAVGRENKAIISQHIGDLKNFETYEFYCESIERFKRMFIFEPR
                     LVVSDLHPDYLSTRFAYETKLPHVKVQHHHAHIASCMAEHNLDEKVIGVSFDGTGLGD
                     DNNIWGSEFFICDLAGYARVNHFDYMPMPGGDKVTEEPWRMAVSYLYKYFGQNFYEFN
                     LPFLNSISTSSVEMLCQAIDKKINSPLSSGCGRLFDAVAALTNLCPVSRFHAEAPMRL
                     EAVMDKNNKESYHFSIHPAISFCETFRQIIADINKGIPVSTISAKFHNTILSVIMAVA
                     NDTRSTTGITKVVLSGGTFQNKYLLGSIEHELTRQGYEVFTHKKVPTNDAGIALGQMA
                     IAAKRRTME"
     gene            complement(3387..>5053)
                     /locus_tag="PLI16_05720"
     CDS             complement(3387..>5053)
                     /locus_tag="PLI16_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84093.1"
                     /translation="EQEKQGIIIEDDCVLQLFDKTTGRYKVEWYMYVESGKLGYFNLL
                     NDFNGSNSIWGFQAYIYHDSIFVDANGSYAASTTFASNSWVKLHIIIDLDDDYATFYK
                     DDTELVSYQWSKGPFGEGSTVKLDAVNFFGWDGSDSPVEGGQSGYYIDDITIESLTVP
                     STPPMNLSAVLNGSDIDVSWSATTPAADLYKLSCNGAVVSATTALTYTEVGPWPDTYI
                     YAVRAKFGPLGYTHSSNTDTVTIAGGVTRDLVLFEEGTSINCGYCPYAAKGIDSLIMV
                     THKEAVAIAYHPDSQWGSFADNYSNPSSVSRLIYYQVTGFPSVISDGKWRINGVTTPY
                     GRQYSSVYLPFYNDRIDAVSFHTLNLVVTPTGTDTYTATITAEETFSAFSSVKLHVVL
                     TESDIPVTWGGLTDIDFVCRGMFPDANGTTIDFSSQSTQNFDINFSTAGFVKEKCQLV
                     VFIQDPVTKLVTQTAKYDMSAATGMDEINGNSISLYPNPATDYIIANTSGTGLLEIVD
                     ITGKLVLRKNILKTSQTIDIRSLEKGMYIVKVTSEKNTFSEKLIIE"
BASE COUNT         1329 a         1109 c         1121 g         1494 t
ORIGIN      
        1 cggctcaaat ccggaaataa cacagcctac cttgtattta acagggaatt catcatagat
       61 gtgcgagcct gtgatggcgc taacgtgtcc cggcgctaaa tacccgttta ttttaatccc
      121 ttcgtcgatt agcgccgaca tggcaggagg catgatcttg tgggagctga atagaaaaaa
      181 atttgtaagg ttttcttttt ttgcattaaa tactcctgcg gcgctgctgc tggcagtagc
      241 ttcaaaacct atacccagaa aaattacttt ttttgatggg tttacgcggg caattttcaa
      301 tgcatcgagt atggaataca ctatcctgat gtcggcacct ttactttttt caaggttcaa
      361 cgaggaggtg gagccgggaa cccggatcaa atcgccaaaa gtggtaataa tagtatcatt
      421 cagcctgcta taagcaattg catgatcgat gtatctcctg ccggtaacac ataccggaca
      481 gccggggccg gatatcaacc ggacttcttt tggcatcagc gaagggatgc caaatttttg
      541 tatggcccag gtatggcctc cgcatacctc catcaggtta atctcttttt ttgcctcagc
      601 gcgtatcttt tcggtcagaa tctttaccaa ttccgcattc ctgaactcat caatatactt
      661 catctgaaaa aattatgaga atatcaatca accttatttt ccgaaggctt tatttcacct
      721 tctggttcgc ccagcttttg caaaagcttc aaagtttcct cagcttcctc ggggcttatc
      781 ttttcgatgg caaaaccggt atgaagcaat acataatcac ctacttgtat gtcggtcagt
      841 aactgcaacg aagcctctac acgggtgccg ccaaccgaaa caatggcgtt atcaccgtcg
      901 atagaaacaa tttttgatgg tattcccagg cacattattt tgtcgtttta taactgcaaa
      961 aatactcaaa atactgcaaa tgtttgtatc ccaaatgaat tatcccactt atttattgtg
     1021 tacactaaag gttattccat ggtccttctt ttggcggcaa ttgccatctg tcccaaagct
     1081 attcctgcat cgttggttgg cacttttttg tgggtgaata cttcataacc ctgacgggta
     1141 agttcgtgct caatagagcc taaaagatac ttgttttgaa atgttcctcc tgataatact
     1201 actttcgtaa tccctgtggt gcttcttgta tcatttgcaa ctgccatgat cactgacaat
     1261 atggtgttgt gaaattttgc tgaaatggtc gaaacaggaa tgcccttatt tatgtcggcg
     1321 ataatttgcc tgaaagtttc gcagaaggaa atcgcaggat gtattgaaaa atggtaagat
     1381 tccttattgt ttttatccat aacagcttcc aggcgcatgg gcgcttccgc atggaaccgg
     1441 ctgaccgggc aaaggttggt gagagcggca acggcatcaa ataatcgtcc gcatccggaa
     1501 cttagcgggc tgttgatttt tttatctatg gcctggcata gcatttcaac agatgaggtg
     1561 ctgatactat taagaaacgg caaattaaat tcgtagaagt tttgtccaaa atatttatac
     1621 agataggata ctgccatccg ccagggctct tcagtaacct tatcgcctcc cggcataggc
     1681 atatagtcaa aatggttgac ccttgcatat cctgcaaggt cgcagataaa aaattcgctt
     1741 ccccagatgt tgttgtcgtc gccgagtccg gtgccgtcga aactaacgcc tataactttt
     1801 tcgtcaagat tgtgttcggc catacaggat gcaatatgcg catgatgatg ttgcaccttc
     1861 acatgcggaa gtttagtttc ataagcaaag cgtgtggaca ggtaatcggg gtggaggtcg
     1921 ctgactacca ggcgtggttc aaaaataaac atcctcttga accgttcaat gctttcacaa
     1981 taaaactcat aggtctcaaa gtttttcaaa tccccgatat gttggcttat gattgctttg
     2041 ttttctctgc caacagcgaa acagttgaca agctcggctc ctgtggctaa aataccgtct
     2101 acatctgttg tcagtttcag cggtgtcggg acataacccc ttgatctgcg gataagtctt
     2161 tcatgcttat tgactatcgt taccactgaa tcgtcagtac ggttgtatat ggcacggttg
     2221 tatgaaagta tggcatcggt aatgtctgaa agtttttcga tggcaatttc attgtcggta
     2281 atgattggct cgtctgaaat gtttccgctg gtcagcacga tagcggggat agttaatcct
     2341 ctgaaaagca aatagtgaaa gggcatataa ggcaacatac agccgatggt gtcgaaaccg
     2401 acattcaccg atggtgccag acttttcttt tgatacagga gcacgatggg gcgcctgtaa
     2461 gatacaagcg acttttcttc ctgtgcattt acgaaagcat attctttcag agtttccata
     2521 ccggagaaca tcacggcaaa aggtttccct tctctgtttt tcatttttcg gagtcgcgct
     2581 acggtctctt cacaaacagc attacaggca ataaaaaaac ctccgatacc ctttatggcc
     2641 actatctttt tgttttgcaa aaggtattgt accctcgaaa caatattttc tatgcctttc
     2701 aattctttct tgccctcaaa gagggtgtat tcgggcccgc aaaccgagca agccaccggc
     2761 tgtgcatgaa accggcggtc gatgacatcc aggtattctt ttttgcagtc gtcacacatc
     2821 acaaaagggc tcatggtggt tttggccctg tcgtaaggaa tattttgtat tagcgtgaaa
     2881 cgcggcccgc aattggtgca gtttatgaaa gtatagtcga tgcggttttg tcgtgttttc
     2941 atgtcccgga gacagtcttc acatacggca atgtcggggc tgatgtcggt aatgcggttc
     3001 gaaaccgttc tgctttttac gatttgaaaa tcatcaaaaa atttggtgtc ggtaattcgg
     3061 aaatctaccg attcaatggc ggctgcctgg ggcgcctgtt cccatatcgc ctgtataaaa
     3121 ctctttacag ttttctcatc gcaattcacc acgatgaaca cgccgtcgtt acggttttcg
     3181 acttgcccgt tcagatgaaa accggcagct atacggtata tgaacggccg gaatcctacc
     3241 ccttgtacca agccttttat ttcgatgaga tacgtattca cgcgctggcc ttagtttagt
     3301 atttgttttt tggcaaaact acaaaatatt aacacaaaag aggcccgcaa aattgcaggc
     3361 ctcttgggta aaaaattcac gtttttttat tcgatgatga gtttttcaga gaaagtgttt
     3421 ttttctgaag taacttttac aatatacatg cctttttcca gactgcgaat gtcaatggtt
     3481 tgagatgttt tcaaaatgtt ttttctcagt accagtttcc cggtgatgtc aacaatctca
     3541 agtaagcctg ttccgctggt attggcaatt atgtaatctg ttgccgggtt ggggtagagg
     3601 ctgatgctgt tgccgttgat ttcatccatg ccggtagcag ccgacatatc gtatttggcg
     3661 gtttgtgtta ccaatttggt cacgggatcc tggatgaaca caaccaactg acatttttct
     3721 ttcacaaaac ctgcggtgga gaagttgatg tcaaagtttt gtgtgctttg cgaagaaaaa
     3781 tctatggtag tcccattagc atccgggaac attcccctgc acacaaaatc aatatctgtc
     3841 aggccgcccc acgtaacagg gatatccgat tcagtaagga ctacatgcag ttttacggat
     3901 gaaaatgcgc taaaggtttc ttcagctgtt atagtagctg tgtaagtgtc ggtacctgtc
     3961 ggggtaacta ccaggttaag ggtgtggaag gataccgcat cgatacggtc gttataaaaa
     4021 ggcagataca ctgaactgta ttgacgtcca tagggtgtgg taacgccatt tatcctccat
     4081 ttgccatcgg aaataacact cggaaatccc gtcacctgat aataaatcaa ccgactgaca
     4141 gaagaagggt tactgtaatt atcagcaaaa cttccccact gggaatccgg atgatatgca
     4201 atggcaacgg cttctttatg ggttaccatg atcaggctgt cgataccttt tgcagcataa
     4261 ggacaatagc cacagttgat cgaagtgcct tcttcaaata gtacaagatc gcgggtaacg
     4321 ccaccggcaa tcgtaacggt gtcggtgttt gaggaatggg tatatccaag cggaccgaat
     4381 ttggctctga cagcgtaaat ataagtgtcg ggccagggac ccacttctgt gtaagtgagt
     4441 gctgtggttg cacttactac ggcaccgttg cagcttaatt tataaagatc ggcggcgggg
     4501 gtggtggccg accaggacac atctatgtcg ctgccattta ataccgcgct gaggttcatc
     4561 ggaggagtgg atggtactgt taatgattca atagtaatat catcgatata gtaacctgac
     4621 tgaccgcctt cgacaggact gtcagaaccg tcccatccaa agaaatttac tgcatcgagt
     4681 ttaacggtag aaccttcgcc aaaaggacct ttgctccact ggtagctgac aagttccgta
     4741 tcgtctttat agaaggtcgc atagtcgtca tcaaggtcga tgattatatg caattttacc
     4801 cagctattgg aagcgaaagt tgtggaagcg gcatagctgc cattggcatc aacaaaaatt
     4861 gaatcgtgat agatatacgc ctggaatccc cagatactat tggatccatt gaaatcattc
     4921 agcaggttga aatatcccag ttttcctgat tctacataca tataccattc aactttgtag
     4981 cggccggttg ttttatcaaa taattgcagc acacaatcat cttcaatgat gattccctgc
     5041 ttttcctgct caa
//
LOCUS       DAOSFP010000156         5012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14909_length_5012_cov_4.609038, whole genome shotgun sequence.
ACCESSION   DAOSFP010000156 DAOSFP010000000
VERSION     DAOSFP010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14909_length_5012_cov_4.609038"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLI16_05725"
     CDS             complement(<1..897)
                     /locus_tag="PLI16_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOH84094.1"
                     /translation="MQKNDKLTDWLPISKEELDKRGWDYVDVIIVSGDAYVDHPSFGH
                     AMIARLLEKEGLRVAILPQPNWRDDLRDFKKLGKPRLFFGVTAGCMDSMVNHYTAFKR
                     LRSNDAYTPGDKAGFRPDYASVVYSNILKRLYPDVPVVLGGVEASLRRLTHYDYWSDL
                     LKPSILIDSGADLLIYGQGINPLKELVKLLQNGGSINSVQQIRQIAYVVEPGKLVGDE
                     NSIMLPAHGDCLKDKRKFAESFCTIETESNRMRAKRLIQPCGDRVTVVNPQYLPMTAA
                     ELDSAYDLPYTRMPHPRYLKRGI"
     gene            1112..2047
                     /locus_tag="PLI16_05730"
     CDS             1112..2047
                     /locus_tag="PLI16_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH84095.1"
                     /translation="MKILIASKIHPFFVEKMQQTGHECDVKILKNSQELEPLIAGYHG
                     VVVNSRFIIDKELVDKATQLKFLARIGSGMESIDTEYLDQKGIRWFNSPEGNRQAVGE
                     HTLGLLLNLLNKITLADRQVRGGLWIREENRGTELPGKTVAIIGYGNMGSAFARCLLG
                     FDMNVIAYDKYKFGYSDENVCEKSLEEVFEQADIVSMHVPLTDETHYMVDDSFINSFR
                     KNIYIINTSRGRVLRTADLVENLKSGKVLGAALDVLEYESASFEKIGLEKFPEPMQYL
                     VEAENVILTPHIAGWTVESNVKHALVLAEKILKAF"
     gene            2407..3639
                     /locus_tag="PLI16_05735"
     CDS             2407..3639
                     /locus_tag="PLI16_05735"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOH84096.1"
                     /translation="MKNSHKIFEGKTLAILTGGGDTPALNSSIESIRNRASILGFKVY
                     GIRKGWKGLLGDGDIVDLSHQPYDGFYGGTALLSSRTNPFPSKKNPENRVPQILQNLK
                     KYKIDVIVTIGGDDTNGAAKRLYETEGIPVIGFPKTIDNDLRTRTMHHYNKKNIEAVL
                     CPGFPSAAKGLMEFTDRIRTTAYSHSRIIVLEVMGRDAGWLTGSAVYGGADLALVPEY
                     EMTKERKDFFFETVKEAYLSSRKKALVIAVSEGVRWYDDQKQKVDVVHASSEVDEYGH
                     PRFGGISGVIASEISNKIKIEARAQISGYYARAGQCRMYDRRLTQALADKVVDLLLRK
                     EYGQMPVMSKLVRYPELEEFNTSSVDMGSIGNKPLPEVYYDINEFNFTPAYKDFLTYI
                     LGEQHFLNFDTDFPVVVV"
     gene            complement(3793..4608)
                     /gene="lgt"
                     /locus_tag="PLI16_05740"
     CDS             complement(3793..4608)
                     /gene="lgt"
                     /locus_tag="PLI16_05740"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOH84097.1"
                     /translation="MNTLFITWNPDAEIFSIGNFGLRWYGFLFAMGFFLAYTVLYFVF
                     KREKVALSKLDWLTFYIFIAVLVGARLGHVFFYEWGYYRENLSEIPMVWKGGLASHGA
                     AIAIIIALIIYVKQFKVNVWWLFDRMALAIPVTAAFVRLGNLMNSEIYGLPTSMPWGF
                     IFRADPAAGLLPRHPTQLYEAISYLLIALVLIILYRKNLGINKPGLFVGIFLSALFTA
                     RFLIEFFKEVQVDFEHGMRFDMGQWLSVPFILLGLGFIVFALKNKPKVADKKL"
     gene            complement(4613..>5012)
                     /locus_tag="PLI16_05745"
     CDS             complement(4613..>5012)
                     /locus_tag="PLI16_05745"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOH84098.1"
                     /translation="EMSEPRFRKDGVLKFLKNNTTVQTILIEIADNDIERTQGLMYRK
                     SMPDSCGMLFIFETMQPLSFWMKNTYIPLDIIYLDNAYRIVSIAPYTQPLSEEAIPSG
                     SEARYVVEVNAGFCDRHKVVVGDKIEFNLN"
BASE COUNT         1438 a         1092 c         1178 g         1304 t
ORIGIN      
        1 gatgcctctt ttcaggtagc ggggatgtgg catgcgcgtg tagggcaggt cgtaggcact
       61 gtcaagttcg gctgcggtca tgggcaggta ttgtgggttt accacggtaa ccctgtctcc
      121 gcaaggctgt atcagccgtt tagcgcgcat tctgtttgat tcggtttcga tggtgcagaa
      181 gctttctgca aatttccttt tgtctttcag gcaatcccca tgggctggga gcatgatgga
      241 attttcatcg ccaactagtt tgccgggttc cacaacataa gctatctgcc ttatctgttg
      301 cacggagttg atggaacccc cgttctgcag gagttttacc agctctttca atggatttat
      361 tccttggccg tagatcagca ggtcggcgcc ggagtcaatc agtatggagg gcttcaaaag
      421 atccgaccaa tagtcgtaat gtgtcaggcg gcgcagcgag gcttccacgc cgcccagcac
      481 cacaggaacg tcgggatata atcttttgag gatgttgctg taaacgaccg aagcatagtc
      541 gggcctgaat cctgctttat ctccgggtgt gtaggcatcg ttgctgcgta agcgtttgaa
      601 agcggtatag tggtttacca tggaatccat gcagcctgca gtaacgccga aaaacaagcg
      661 tggtttgcca agctttttaa aatccctgag gtcgtcgcgc cagttgggtt ggggaaggat
      721 tgccacccgc aggccttctt tctcaagaag ccttgctatc atagcgtggc cgaaagatgg
      781 atgatctacg taggcgtcgc cgctgacaat aatcacatcc acataatccc atccgcgttt
      841 gtcgagttct tctttactta tcggaagcca atcggtaagc ttgtcgtttt tttgcatata
      901 tacaaagata agtgaaattg tgaatatcaa attactttgc gtgagtgcgt ctttgcaaga
      961 aaataatacg ttggattttt ttcggatttt tacaacatgg gcacatgtgc ataatgaaac
     1021 actgtaaaac agattaaatg tctttattat ttgtctttct aaaaattcta taacggcata
     1081 ttttatctaa atttgcagta aatacaaaaa aatgaaaatc cttattgcat cgaaaataca
     1141 tccttttttt gttgagaaaa tgcagcagac agggcatgag tgtgatgtga aaatcctgaa
     1201 aaattcacaa gaacttgaac cgctaatcgc cggttatcat ggggttgtgg tgaacagccg
     1261 ttttattatt gataaagagc tggtcgataa agccacacaa ctgaaatttc ttgcccgcat
     1321 aggatcggga atggagagta ttgacacgga gtaccttgat cagaaaggta tccgatggtt
     1381 caattctcct gaaggaaacc gccaggcggt tggcgaacac acattgggat tgcttttgaa
     1441 tttattgaat aaaataactc ttgccgacag gcaggttcgt ggcggattgt ggatacgtga
     1501 ggaaaatcgc ggaacggaac ttccgggaaa aactgtagct atcatcggct acggcaatat
     1561 gggaagcgct tttgcccgtt gtttgcttgg tttcgatatg aatgtgatag cttacgacaa
     1621 atataaattt gggtattctg atgaaaatgt ctgcgaaaaa tcgctggagg aggtgtttga
     1681 acaggccgat attgtgagta tgcatgtccc gctgaccgat gaaacgcatt atatggtgga
     1741 tgacagcttc attaattctt tcagaaagaa catatacatc atcaacactt cgcgcggtag
     1801 agtattgaga acggctgacc tggttgaaaa cctgaaaagc ggcaaggtgc ttggtgcagc
     1861 cctcgatgtg cttgagtacg aatccgcttc ttttgaaaaa atcggtctgg aaaaattccc
     1921 tgagccaatg caataccttg tagaagcaga aaatgtgatt ttaacaccgc atattgccgg
     1981 atggacagtg gaatcaaatg ttaaacatgc gctggtactg gcggagaaga ttttgaaagc
     2041 tttttaatct gcgagcatcg gcggaatcag tggaaaaaga aatgaagaaa ttgtcccgca
     2101 gttagcgcag attgacacag aaacacaagg agagaagaat caatattttt gaattttctg
     2161 acaaacatgc agtttatttg ataggcaaag cctataagaa taaaaagacg gtagtacaac
     2221 cgctgccgaa ccagggaaga tattaaaggc tttttaatct gcgaacatca gcgtaatccg
     2281 cgggaaaaga aatgaagaaa ttctcgcgca gtctgccatg tcggacctaa tagcctaacc
     2341 ccctaaaaat cccctctcca tcaaaaaaaa tatcctatat ttgcctgtca aaaaaaatcc
     2401 agaaaaatga aaaacagtca caaaatattt gagggaaaaa ccctggcgat actcacaggc
     2461 ggaggcgaca cccctgctct caattcaagc atagaatcca tccgaaacag ggcttccatc
     2521 ctcggtttta aggtatatgg catccgcaag ggttggaaag gtctgctggg cgatggcgac
     2581 atcgtagatt tatcacacca gccttatgat ggtttttatg gcgggacagc ccttctttca
     2641 agccgcacca atcctttccc atcaaagaaa aaccccgaaa accgggtccc ccagatttta
     2701 cagaatctga aaaagtataa aatagatgtg atagttacca tcggcggtga tgacaccaac
     2761 ggcgcagcca aaagactgta tgaaacagaa ggtattccgg ttattggctt cccaaaaaca
     2821 attgataatg acctgcggac aaggaccatg catcattata acaaaaaaaa catcgaagcc
     2881 gtgctttgtc cgggtttccc atctgccgcc aaagggctta tggagttcac cgaccgtatc
     2941 cgcaccactg cctattcaca ttcgcgtatc attgtgcttg aggtgatggg gcgtgatgcc
     3001 ggctggctta ccggcagtgc tgtgtatggc ggcgccgacc tggcccttgt ccctgaatac
     3061 gagatgacca aagaacgcaa ggatttcttt tttgaaaccg tgaaagaggc ctacctgagt
     3121 tcccgcaaaa aagccctcgt gatagctgtt tcagaaggcg tccgctggta cgatgaccaa
     3181 aaacaaaaag tggatgtggt acatgccagc tccgaagttg atgaatacgg gcatcccagg
     3241 tttggcggca tcagcggtgt catagcttcg gaaataagca ataagattaa gatagaagcc
     3301 cgtgcccaga tctccggcta ttacgcccgc gccggccaat gcaggatgta tgaccgccgg
     3361 ctcacccagg ccctggccga taaggtggtg gatctattgc ttcgcaagga atacggccaa
     3421 atgcccgtga tgtctaaatt ggttagatac cctgaacttg aagaattcaa cacctcttct
     3481 gttgatatgg gatccattgg caataagcct ttgcctgagg tttattacga tatcaacgag
     3541 tttaatttta caccggcata taaagacttt ttaacctata ttttgggtga acagcatttc
     3601 ctgaattttg atactgattt ccctgtggtc gttgtgtaac ctgaagtttt attcagggta
     3661 aacgatgccc ggtgaaaaca ggagattgtt attcacttca aaacaggtgt agtgataacg
     3721 gatcagcctt acgctaattc atctttactg ttaatccttg aaaacaactt ttgccgaaag
     3781 cagctgaaaa aatcagagct ttttgtcagc aacctttggt ttgtttttca aagcaaaaac
     3841 gataaaccct aacccaagca ggataaaggg tacgcttagc cattgcccca tatcaaacct
     3901 cattccgtgt tcaaaatcca cctgcacttc tttgaaaaac tctatcagaa aacgggcggt
     3961 gaacaaagct gacaagaaaa tgccaacaaa taatcccggt ttgttaatgc ccaggttttt
     4021 gcgataaagg ataatcagta ccagggcaat cagcaggtaa gaaatggctt catagagttg
     4081 ggtgggatgc ctgggcagaa gtcctgcagc gggatcggcc ctgaaaataa aaccccaggg
     4141 cattgaggtg gggaggccgt atatttccga gttcatgagg tttccaagcc ggacaaaagc
     4201 cgcggtaaca ggtatggcca gcgccatgcg gtcgaagagc caccacacat tgaccttaaa
     4261 ctgttttaca tagataatca gtgcgatgat aatggctatg gcggcgccgt ggcttgccag
     4321 cccgcctttc cacaccatgg gtatctcgct gaggttttca cggtaatatc cccattcata
     4381 aaagaataca tgtcccagtc gggccccgac aaggacagcg ataaagatgt aaaaggtaag
     4441 ccaatcgagt tttgacaagg ctactttttc gcgtttaaag acaaaataca gcactgtata
     4501 agccagaaaa aaacccatgg caaacaaaaa gccataccag cgcagaccaa aatttccaat
     4561 ggaaaaaatt tctgcgtcgg gattccaggt aataaataat gtgttcatac catcagttta
     4621 aattaaattc tattttgtca ccgacaacta ccttgtgtct gtcgcagaac ccggcattca
     4681 cttccaccac atacctggct tcgctccctg agggtattgc ttcttcggac aacggctgtg
     4741 tgtaaggagc tatgctcaca atacggtacg cgttgtcgag ataaataata tcgagaggta
     4801 tataagtgtt tttcatccag aaagacaaag gttgcatagt ttcaaagata aacaacatcc
     4861 cgcaactgtc gggcatggat ttgcggtaca tcagaccctg tgtcctttct atgtcattgt
     4921 cagcaatttc gatgaggata gtctgcaccg tggtattgtt tttcaagaac ttcaacaccc
     4981 cgtcctttct aaagcggggt tcagacatct ct
//
LOCUS       DAOSFP010000157         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_14967_length_4995_cov_3.857490, whole genome shotgun sequence.
ACCESSION   DAOSFP010000157 DAOSFP010000000
VERSION     DAOSFP010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14967_length_4995_cov_3.857490"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLI16_05750"
     CDS             complement(<1..346)
                     /locus_tag="PLI16_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOH84099.1"
                     /translation="MYILLCSNGKYYTGSTKDLEKRWQEHLSGNGANFTKKYPPVKLV
                     YYEEYDRIDKAFFREKQVQGWSRKKKEALINGENKKLHDFAECMNESHYKNKRLASTP
                     LSKPNSANGNDSG"
     gene            complement(444..3212)
                     /locus_tag="PLI16_05755"
     CDS             complement(444..3212)
                     /locus_tag="PLI16_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOH84100.1"
                     /translation="MSQEINQNPEQKARDNIDKQLITCGWNVQNKEQINLGSGIGVAV
                     REYLTDVGPADYVLFVDRKPVGIIEAKREEEGVRLTTVEDQSSDYARSKLKHLNNDPL
                     PYVYESTGELTRFTDYTDPKPRSRPVFTFFKPETFREWQRKTASLRKRLLNLPALPTN
                     GLRECQILAIKKLDSSFKENKPKALIQMATGSGKTFTAITFIYRLLKFCDAKRVLFLV
                     DTKNLGEQAEQEFMSFLPPDDNRKFTELYNVQRLRSSYVATDSQVCISTIQRLYSILK
                     EEELDETAEETNPGEFLWEKREPVPVGYNAKVPIEFFDFVVIDECHRSIYNLWKQVLE
                     YFDAFLIGLTATPDNRTFGFFNQNIVSEYTHEQAVIDGVNVGFNVYLIETVISKRGGV
                     IWKGEYVDFREKLSRKKRWEQLDEDLEYSAKKLDDTVVNPNQIREIIRTFKEQLPEIF
                     PHREEVPKTLIFAKTDSHADDIIQIVREEFAEENRFCKKITYNADDPKSVLAQFRNDY
                     YPRIAVTVDMIATGTDVKPLEILLFMRDVKSRNYFEQMKGRGTRVIELDDLKKVTPSA
                     QYTKDHFVIVDAVGVTKSLKTDSRPLEKKPGVPLKDLLMAITVGARDEALFTSLANRL
                     IRLDRQVSETERAKFAEKAGGKTITEVAKELLVAYNPDTLDEIGAHIVKNKPSATTAE
                     IDTALKQAQDELIEKAAATFHGELNNYIENVRKAHEQVIDIINPDKLEFAGWEEQSAE
                     YANKIISDFKAYIEANKEEITALQIFYGQPYRRRELTYKMIKELLEQIKADIPAMAPM
                     KVWHAYAAIEQTNSSPKNELVALVSLVRRMCELDSTLTPYDKTVDKNFQDWVWKKHSG
                     AGEKFNEEQMHWLRMIKDYVATSFHIAKDDFDLSPFNAQGGLGKMWQLFGDKTEEIIN
                     ELNEVLAA"
     gene            complement(3679..>4995)
                     /locus_tag="PLI16_05760"
     CDS             complement(3679..>4995)
                     /locus_tag="PLI16_05760"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84101.1"
                     /translation="AKAFQGNPAPNLPTYNSVKYDIGKVIVILKSYPAGVELARDTSN
                     TTGDYEFNDVLDGTYKISYDKYTADTMQTGNEINAVDIAMLKYFIGHDTLFDPSRNFS
                     MKHKKAINIDNNASINAVDAGRLKAKVGQPYLPVANFSKGNWVAFDTVVTVAGANLNV
                     ILKTICYGDYDASSTKYRDSTLTWSMAKSEPEDNIIQLSEETMSISGPGYFEVPLKAS
                     SKMYELSALGLELSYPNKKFKLVSASMPKASVKEGEAKINPPLQEIIADNKDLLVTDL
                     DGVIRVVYATTDYFDVAAGEEIIRLGFLMVEKTERGEVDFTLNGTGIMADQYGKEYDG
                     AYLTMPRVFMQDDKMDALLTLSGYPNPFGNTVNLIYTLPENGTVKINVYNSLGELVRK
                     IADGEEMSGKHILEIPTAQLPHGLYTFKMEFSGDKVSDCRLMKMIH"
BASE COUNT         1237 a         1145 c          997 g         1616 t
ORIGIN      
        1 aaccactgtc attaccgttc gctgagttgg gcttgctgag cggagtcgaa gcaagcctct
       61 tgttcttgta atgactttca ttcatacatt ctgcaaaatc atgtaatttt ttattttctc
      121 cgtttatcaa cgcttccttt ttctttctgc tccaaccctg aacctgtttt tcacgaaaga
      181 atgctttgtc gatcctatca tattcctcat aataaaccag ttttaccgga ggatattttt
      241 ttgtaaagtt ggctccattt ccgctcagat gttcctgcca gcgtttttca aggtctttgg
      301 tacttccggt ataatatttt ccgtttgaac aaagcaaaat gtacatgtat cctttcattt
      361 ttcatttatt agttacggtg gtttcggcta cgctcaacca ccgtcctccc cccgggcgtt
      421 gagcgcagtc gaaacgcccg attctaagca gccaaaactt catttaattc attaataatt
      481 tcttctgttt tgtctccaaa taactgccac atcttgccca ggccgccctg tgcattaaaa
      541 ggactaaggt caaaatcatc ctttgcaata tgaaaagatg tggccacata atcttttatc
      601 attctcagcc agtgcatctg ctcttcatta aatttctctc cggcgccgga atgttttttc
      661 catacccagt cctggaaatt cttatccaca gttttatcat aaggtgtcag cgtgctgtcg
      721 agttcgcaca tcctgcgaac cagcgatacc aatgcaacca gttcgttttt tggggaactg
      781 ttggtttgtt caattgcggc ataagcatgc cagactttca tcggcgccat ggccggtatg
      841 tctgccttta tttgttccag cagttctttg atcatcttat aggtcagctc acggcggcgg
      901 tagggctgtc cgtaaaaaat ctgcaaggcc gtaatttctt ctttgttggc ttcgatatat
      961 gctttaaaat cgctgataat tttattggca tattcggctg attgttcttc ccatccggca
     1021 aattcaagtt tgtcgggatt gatgatatct ataacctgtt cgtgtgcttt gcggacgttt
     1081 tcaatatagt tattcaattc gccatgaaag gtagcggctg ctttttcgat aagttcatcc
     1141 tgtgcctgtt tcagggcggt gtcaatttcg gcagttgtgg ccgatggctt gtttttaaca
     1201 atatgtgcgc ctatttcatc cagtgtgtcg gggttgtatg caacaagtaa ttctttagca
     1261 acctcggtta tggttttgcc cccggctttt tctgcaaatt ttgccctttc tgtttcgctg
     1321 acctgccggt cgagccgtat caggcggttg gcaagtgagg taaacaatgc ttcatcacgt
     1381 gcccccacag ttatggccat cagcaagtcc ttcagcggca caccgggttt tttctccagc
     1441 ggccggctgt ctgttttcag cgattttgtt acgccaacgg catccacaat tacaaaatgg
     1501 tctttggtgt attgtgccga aggggttact tttttcaggt cgtccagttc aatcacacgt
     1561 gttccgcggc ctttcatctg ttcaaaataa ttgcggcttt ttacgtcacg cataaacagc
     1621 aatatctcta acggctttac atcggtgccg gtagcaatca tatccacggt aactgctata
     1681 cgcgggtaat aatcattgcg gaactgtgcc agcaccgatt tgggatcatc ggcattatag
     1741 gtaatttttt tgcaaaaacg gttttcttcg gcaaattctt cacgcacaat ttgaatgata
     1801 tcatcggcat ggctgtcggt tttagcaaag atgagtgttt ttggcacttc ttcacggtgg
     1861 ggaaatattt cgggcagttg ctctttaaag gtacggatga tttcacggat ctggttgggg
     1921 tttaccacgg tatcgtccag ctttttggca gaatattcca agtcttcatc cagttgttcc
     1981 cagcgttttt ttctgcttaa tttctcgcga aaatccacat attccccttt ccatataacg
     2041 ccacctcgtt ttgatatgac tgtttcaata aggtaaacat tgaacccgac attcacacca
     2101 tcaatcacag cctgctcgtg ggtatattcg cttacgatat tctgattaaa aaaaccaaag
     2161 gttcggttat cgggagtggc tgtaagtcca atgagaaaag catcaaaata ttcaagcacc
     2221 tgtttccaca ggttgtagat gctgcggtgg cattcatcta tcactacaaa atcaaaaaac
     2281 tctatgggaa cttttgcatt atatcccacg ggaacaggct cccgtttttc ccatagaaat
     2341 tctcccggat tagtttcttc tgcggtttcg tcaagttctt cttcttttaa aatagaatac
     2401 aaacgctgta tggtgctgat gcaaacctgg ctgtcggtag ctacataaga ggaacgcagg
     2461 cgctgaacat tataaagttc ggtaaacttc ctgttatcgt cgggtgggag aaagctcata
     2521 aattcctgct ctgcctgttc cccaaggttt ttcgtgtcaa ccaaaaacag cactcgtttt
     2581 gcatcgcaga attttagcaa acggtaaata aatgttatag cggtaaaagt ttttcccgag
     2641 ccggtagcca tctggatcag cgctttgggc ttattttctt taaatgagct gtccagtttt
     2701 tttattgcta aaatttgaca ttcgcggaga ccattagtag gtaatgccgg taggttaagc
     2761 agccttttac gcaacgacgc tgtttttcgc tgccactcgc ggaatgtttc tggtttgaaa
     2821 aacgtaaata cagggcgtga cctgggtttg gggtcggtat aatccgtaaa acgtgtcagt
     2881 tctccggtgc tttcataaac gtaaggcagc gggtcgttat tcaggtgttt aagtttgctt
     2941 ctggcataat ctgatgactg atcttcaacg gttgtcagcc ttacgccttc ttcttctcgt
     3001 ttggcctcaa taattccgac cggcttacga tcaacaaaaa gcacataatc agccgggcca
     3061 acatcggtaa gatattcccg gacagctaca ccaattccgg aaccaagatt tatttgttct
     3121 ttattctgaa cattccaccc gcaggtaata agctgtttgt caatattgtc gcgggctttc
     3181 tgttccgggt tctggttaat ttcctgactc attttttatt aatcagaccc ctaaaataca
     3241 aaaattgtca atgtgtgtaa atattatcag gaataataaa cgatttgtgt tcataaaagc
     3301 agggtgtggt ttatcgactc aaaagcagca cctctcaatc cattctaatg aagaataata
     3361 ccaattggaa taataatata atccaaactt cgcttcatac ttttgcacta tattattatt
     3421 ttaaccattg gcataaccct gtcagactgt cgcagacagg caggcatatg agaaatatca
     3481 aaagtaccgt ataactcaaa taggccggaa tcctttctgt taccaggctc gtacaccacc
     3541 ttgcatcccg gttggtatta cttttataac gctttcacaa acaataaaac cctgtttatt
     3601 aatccccggc tttgtatttt atcaaagttc cagagtattt tttgtctcaa atcgagtcgg
     3661 atcagttgcg gggtcatttc aatgtatcat cttcattaac ctgcaatctg aaactttatc
     3721 tccggaaaac tccatcttaa aagtatataa tccatgcggt aattgggctg ttggaatctc
     3781 taaaatatgc ttgccgctca tttcttcacc gtccgcaatt ttcctcacca attcacctaa
     3841 agaattatat acattgattt ttacagtccc gttttccggc aaagtataaa tcaggtttac
     3901 tgtgttaccg aaaggattgg gataccctga caaagttagt aaggcatcca ttttgtcatc
     3961 ctgcataaaa accctgggca tcgtaagata ggcgccatca tattcttttc cgtactggtc
     4021 ggccataatt ccggttccat tcaaggtaaa atccacctct cccctttctg ttttctcaac
     4081 catcaaaaag cccagcctga ttatttcttc accggcggcc acatcaaaat aatcagttgt
     4141 ggcatacacc accctgataa ccccgtccag gtcggttacc agcaggtctt tgttgtcggc
     4201 aatgatttcc tgcaatggcg gatttatttt tgcttcacct tctttaacgg aagcttttgg
     4261 catggaagcg cttactaatt tgaatttttt attcggataa ctcaattcca gacccaaagc
     4321 agataactca tacattttgg agcttgcctt cagcggaacc tcaaaatacc cgggaccact
     4381 gatactcata gtttcttcgg aaagctgaat gatgttatcc tccggttctg attttgccat
     4441 actccaggta agtgtcgaat ccctgtattt ggtactcgac gcatcgtaat cgccataaca
     4501 gatggttttc agtataacat tcaaattggc gcctgctaca gttaccaccg tgtcaaaggc
     4561 cacccagttt cccttggaaa aattcgcaac aggaaggtat ggctggccaa cctttgcttt
     4621 gagccttccg gcatcaaccg cgttgataga tgcattgtta tcgatattta ttgctttttt
     4681 atgtttcatg gaaaagtttc tcgacgggtc aaacaacgtg tcgtggccta taaaatattt
     4741 gagcatggca atatctaccg cattaatctc atttccagtc tgcatggtgt ctgccgtgta
     4801 tttatcgtat gaaattttgt aggtcccatc aagaacgtcg ttaaactcat agtcgccggt
     4861 agtgttgctg gtatcccttg ccagctctac tcctgccggg taacttttca gtatcacaat
     4921 gactttgcca atgtcgtact taaccgaatt ataggtggga agattcgggg caggatttcc
     4981 ctggaaggct tttgc
//
LOCUS       DAOSFP010000158         4975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15049_length_4975_cov_3.851626, whole genome shotgun sequence.
ACCESSION   DAOSFP010000158 DAOSFP010000000
VERSION     DAOSFP010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15049_length_4975_cov_3.851626"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLI16_05765"
     CDS             complement(<1..833)
                     /locus_tag="PLI16_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014298623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase"
                     /protein_id="HOH84102.1"
                     /translation="MQKLMLLGDEAIAQGAIDAGISGVYAYPGTPSTEITEYIEHSRE
                     AKEKNIKANWAANEKTAVEGAIGMAYAGKRVITCMKHVGMNVAADAFVNSAITGTNGG
                     MIVVAADDPSMHSSQNEQDSRFYGRFSMLPILEPTNQQEAYDMAHYGFELSEKYHLPV
                     LMRITTRLAHSRSGVVRKPARAQNELKLPEDMKQYVLLPAIARKRYKLLLSNQEKLVQ
                     ESEQSPFNKYVDGQDKSKGIITFGIAYNYLLENYPDRVVPYPVVKISQYPAPFKAIEK
                     L"
     gene            complement(1048..1524)
                     /locus_tag="PLI16_05770"
     CDS             complement(1048..1524)
                     /locus_tag="PLI16_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84103.1"
                     /translation="MLRHSLAEPKQGSMPDFMRPLSLDGIEIIKNLGSFLHKKQVCFD
                     SILASHAVRAYETALYLAETTNVSNDKIVEDRRLYSADLHAYINILFEQPDEYCHLAI
                     VGHNPQITALASYFMKENDLFLPPGAMVAVKIDTDKWTDACLAGSELLFSWTPGNK"
     gene            complement(1597..2877)
                     /gene="glyA"
                     /locus_tag="PLI16_05775"
     CDS             complement(1597..2877)
                     /gene="glyA"
                     /locus_tag="PLI16_05775"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOH84104.1"
                     /translation="MERDVKIFDLIQKEYERQLHGIELIASENFVSQQVLEAMGSVLT
                     NKYAEGYPGKRYYGGCQVVDQTEQIAIDRAKELFGAEWANVQPHSGAQANMAVLMTCL
                     KPGDTFMGLNLSHGGHLSHGSPVNSSGILYKVVDYGVEEETGTVNYDKMEEVALRERP
                     KLIIAGASAYSRDWDFKRMREIADKIGAILMADIAHPAGLIAKGLLNNPVPYCHIITT
                     TTHKTLRGPRGGMIIMGKDFENPWGLKTPKGEIKMMSALLDSAVFPGIQGGPLEHVIA
                     AKAVAFGEALTDSYKNYVIQVKKNAAVMSKCFTDKGYKVISGGTDNHLMLIDLRTKFP
                     EITGKVVENTLVRAHITVNKNMVPFDSRSPFTTSGLRVGTPAVTTRGMKEEHMPIIVD
                     LLDEVIANIENETVIASVGERVQKMMRDFPLFAY"
     gene            3364..4053
                     /locus_tag="PLI16_05780"
     CDS             3364..4053
                     /locus_tag="PLI16_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84105.1"
                     /translation="MNFEKDHIYHIYNQGNNKQKIFFINENYYFFIHKIKTHVLQFAD
                     ILAWCLMPNHFHIMVYVNNPELTFRGTNKSRSQARNETVGVTDNSTGQARSHPDSSKR
                     SLNNSIGIMLRSYTRAVNIQEKRSGALFREETKAECMTNSSGITPAFFNTNNGTLINI
                     PLNAKEYPQICFDYIHNNPVKAGLVANPEEWEFSSFREFFGEKILVNKTRVMQLKLKY
                     LPDIGLQTHFN"
     gene            4079..4633
                     /locus_tag="PLI16_05785"
     CDS             4079..4633
                     /locus_tag="PLI16_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HOH84106.1"
                     /translation="MKTFPKISLTGDLGSGKSHVSRLLCEKLGFRIVSTGGIQREIAA
                     KYHMTTLELNEYTKTHPEIDDEIDGTISKLQEAGESLIFDSRLAWHFAPKSFKVYLTV
                     DINEAARRIFYDKRTSENYQNIEEARDKILARRKSELERFKDYYNIDYSDLGNYDLIV
                     DTTHINPEEVSEKIIKAFRVWEQK"
     gene            4735..>4975
                     /locus_tag="PLI16_05790"
     CDS             4735..>4975
                     /locus_tag="PLI16_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84107.1"
                     /translation="MKIAIIGFGAASIGFIEKIINSRHEFHIFEKSKDIYSSSISGIR
                     ADGKLFVSAEMGGDIPIDLNVQRQLVDFYIQKTKNH"
BASE COUNT         1423 a         1102 c         1047 g         1403 t
ORIGIN      
        1 caaagctttt cgatagcctt aaaaggtgcc ggatattgcg aaattttcac caccggataa
       61 ggcaccaccc tgtcgggata attttcgaga agataattat aagctatgcc gaaagtgatg
      121 attcctttac tcttgtcctg cccatcaaca tatttgttaa aaggcgactg ttcggattcc
      181 tgcaccagtt tctcctggtt gctcagcaag agtttatatc ttttgcgggc gatagccgga
      241 agcagcacat attgtttcat atcttccgga agtttcagtt cgttttgcgc cctggcaggt
      301 ttgcgcacca cgcccgaacg tgaatgggcc agtcgtgtgg taatcctcat caacacaggc
      361 agatgatatt tttcagaaag ttcaaaacca taatgagcca tgtcataagc ttcttgctgg
      421 ttggtcggtt ccagtatagg caacatcgaa aatcttccat aaaatcttga atcctgctca
      481 ttctgtgaag aatgcatgga aggatcgtcg gcagccacta ctatcatccc cccgttggta
      541 ccagtgatag cagaattaac aaaagcatcg gcggcgacat tcatacccac atgtttcata
      601 caagtaatca ctctcttgcc ggcgtaagcc atccctatag cgccttctac tgctgttttt
      661 tcgttggcag cccaattagc cttaatattt ttttctttag cttccctcga atgttcgatg
      721 tattcggtaa tctcagtcga aggggttcca ggataagcat agactcctga aatacccgca
      781 tcgatggcac cctgagctat agcctcatca cccaataaca ttaatttttg catatcttgt
      841 tttatttaat ttaatattcg gtgcttacaa aactacacaa tttttttttc ctgccaagac
      901 aattttccat tttataccga tacgctatca gtaatacctc aaataggccc atagtaccgg
      961 cataccttag actctaacac ccacccgttg cgcaaaacca ttactaaatc ctggttttgt
     1021 tttgggctca taaattatgt aatgttgtta tttatttcct ggagtccagc tgaaaagcaa
     1081 ttcgctgccg gcaagacatg catcggtcca tttatctgta tcgattttca cagcaaccat
     1141 agcgccgggc ggcaggaata aatcattttc tttcataaaa taacttgcca gcgccgtaat
     1201 ttgcggatta tgacctacta tagccaggtg acaatattcg tcgggctgct caaatagaat
     1261 attgatatac gcatgcaaat ctgcagagta caagcgcctg tcttccacta ttttatcatt
     1321 tgacacgttg gtggtttcgg ccaggtacag tgctgtttcg taggcccgca cggcgtggct
     1381 ggcaaggatg ctgtcgaaac aaacttgttt tttgtgaaga aagctgccaa gatttttaat
     1441 gatctctatg ccgtcgagtg acagtggcct cataaaatcg ggcatggaac cctgtttggg
     1501 ctctgccagc gagtgccgta gcatgtacaa atttttcata ggcacaaaaa aagaaaacac
     1561 agggttgtgg ccctgtgttt tcgaaatcag aagttattaa taggcgaaaa gaggaaaatc
     1621 tctcatcatt ttctgaaccc gttcgccgac cgaagctatt acagtttcgt tctcaatatt
     1681 ggcaattacc tcatcgagca gatcaactat aataggcata tgttcttctt tcataccacg
     1741 ggtggtcact gccggtgttc ccacacgcag gcctgaagtg gtgaaaggcg accggctgtc
     1801 gaaaggcacc atatttttgt ttacggtgat gtgtgctctc actaaagtgt tttccactac
     1861 cttaccggta atttccggaa attttgtacg caggtcaatc agcatcaaat ggttgtcggt
     1921 accgccggag atgactttgt atcccttgtc ggtaaagcat ttcgacatta ccgcggcatt
     1981 tttctttacc tgtatcacat agtttttata ggaatcggta agcgcctcgc caaaggctac
     2041 cgctttggca gcaatgacat gttccagcgg cccaccctgt attcccggga acacggctga
     2101 gtcgagcagt gccgacatca tttttatttc gcctttcggt gttttcaatc cccaggggtt
     2161 ttcaaagtct ttgcccatga tgatcatacc gccacgaggc ccgcgcaggg ttttgtgtgt
     2221 ggtggtggtg atgatgtggc aataaggcac ggggttgttg agcaaccctt ttgcaataag
     2281 tcctgcagga tgtgcgatat cggccatcaa tatcgctcct attttgtcgg ctatttcgcg
     2341 catgcgtttg aaatcccagt cgcgtgagta agcagaagcg ccggcgatga taagtttcgg
     2401 gcgttcgcgc agggccactt cttccatttt atcataattc acggtgccgg tttcttcttc
     2461 cacgccgtaa tcaacaactt tatataaaat acccgatgag ttcacgggtg agccatgcga
     2521 aaggtgtccg ccgtgcgata ggttgagccc cataaaagta tcgccgggtt tgaggcaggt
     2581 catgagtact gccatgttgg cctgagcccc tgagtgaggc tgaacatttg cccattcagc
     2641 gccaaaaagt tctttggcac ggtcgatggc tatctgttca gtctggtcca ccacctggca
     2701 accgccataa taccgtttgc cggggtatcc ttcagcatat ttgttggtaa gcaccgagcc
     2761 catggcttcg agtacctgct ggctaacaaa gttttctgaa gcaatcagtt caatgccatg
     2821 caactggcgt tcatattctt tctgtatcag gtcgaatatt tttacatctc tttccattac
     2881 attagttttt aagtttgttt tgcaaatgta taaataaaat ttataaaaaa aattctttac
     2941 cattaaggta gtaacataga aataatgtct ataagaaaac ttgtgtttgt gcaaagaaaa
     3001 cgtcaagttt gaggcctgcc cgactgcgcc agtcaggcgg gcttgcgaag cgacaaaaag
     3061 cggagtcccg acacttcacg tcgggatgag catttttgtt gcaagcgtaa ccctgcccga
     3121 tgcaggcggg gaaaaattga agttttctta taaccacgta ataagacgac tacaccttac
     3181 cccacttaaa aactgtcggg aaacaggcgg gtttgcaatt cataaaaaag acttattttt
     3241 gattttttaa ttgcgacaac tgatgaaaac tttcccaaaa atatccctta caggcgacct
     3301 gggcagcggc aaaagccatg tcagccgcct actgtgcgaa aaacttactt aagaaatctc
     3361 gaaatgaatt ttgaaaaaga ccatatttat cacatttata atcagggaaa taacaaacaa
     3421 aaaatatttt tcataaatga aaattattat tttttcattc ataagattaa aacgcatgta
     3481 ttgcaatttg cagatattct ggcatggtgt ctaatgccaa atcattttca cattatggtt
     3541 tatgtgaata atccagaact tactttcagg ggaactaaca aaagcagaag ccaggcgcgc
     3601 aatgaaactg tcggggtgac tgacaacagc acagggcagg cgcgcagtca ccccgacagt
     3661 agcaaacgct cattaaacaa ttcaattggt attatgctcc gctcatatac ccgggctgtt
     3721 aatattcagg aaaaaagaag tggtgcatta tttcgtgaag aaaccaaagc tgaatgcatg
     3781 acaaattctt caggaataac acctgctttt ttcaatacta ataatgggac attgattaat
     3841 attcctctta atgctaaaga atatcctcaa atttgcttcg actatattca taataatcct
     3901 gtcaaagcag gacttgtcgc taaccctgag gaatgggaat tttcttcttt tcgtgaattt
     3961 ttcggagaga agattcttgt taacaaaaca agggtaatgc agcttaaact taaatatttg
     4021 cccgatattg gcttgcaaac tcactttaat taacttattt attttaaact tactgataat
     4081 gaaaactttc ccgaaaatat cccttacagg cgacctgggt agcggcaaaa gccatgtcag
     4141 ccgcctgctg tgcgaaaaac ttggcttccg cattgtttcg acgggaggca tacaaagaga
     4201 aatagcggca aaataccaca tgactaccct cgaactaaac gaatacacca agacacatcc
     4261 cgaaattgat gacgagattg acggcactat ttcgaagctg caggaagccg gagaatccct
     4321 gatttttgat tcgcgcctgg catggcactt cgcaccaaaa tcttttaaag tatatcttac
     4381 ggtggatatc aatgaagcag cacggagaat tttttacgac aagcgtacca gcgagaatta
     4441 tcagaatatt gaagaagccc gcgacaaaat tcttgcccgc cgcaaaagcg aactggaaag
     4501 gtttaaggat tattacaata ttgactattc cgacctgggc aattacgacc tgatagttga
     4561 taccacacac ataaaccccg aagaagtcag tgaaaaaata atcaaggctt tcagagtatg
     4621 ggagcagaaa taaattattg tgagtaatat tttcccgcag atcgcgcaga tatacgctga
     4681 tgagaatctg cgaaaatcag cggaatcagc gggaactaat aaaaaataca gaaaatgaaa
     4741 atagcaatca tagggtttgg cgccgcttcc attggtttta tcgaaaagat aatcaacagc
     4801 aggcatgagt ttcatatctt cgagaaatcc aaagacatat attcctcaag tatttcgggc
     4861 atacgtgccg acggaaaact attcgtgtcg gcagaaatgg gcggggatat tcctatcgac
     4921 ctgaatgtgc aacggcagtt ggttgatttt tacatccaaa aaaccaaaaa tcacg
//
LOCUS       DAOSFP010000159         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15191_length_4942_cov_4.097811, whole genome shotgun sequence.
ACCESSION   DAOSFP010000159 DAOSFP010000000
VERSION     DAOSFP010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15191_length_4942_cov_4.097811"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1025)
                     /gene="miaA"
                     /locus_tag="PLI16_05795"
     CDS             complement(117..1025)
                     /gene="miaA"
                     /locus_tag="PLI16_05795"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH84108.1"
                     /translation="MITILGPTACGKTRLAVAVAEKNGGEIISADSRQVYRGMTIGTG
                     KDLDDYLVDGKKIPFHLIDVADAGEEYNLFRFVSDFKKVFDEIILRKKTPVLCGGTGM
                     YIEAVLKNYAVPDVPVNEAFRKTLENKTDKELQTLLESYKTLHNETDTENRRRLIRAL
                     EIAHFAAEKKLLPEKLFAGDSYVFGISMPRDMVRMRITDRLHKRLEEGMIDEVKRLLD
                     AGVGADRLIRYGLEYRYITQYLLREFSYDEMVRLLNIAIHQFAKRQMTWFRGMERRGI
                     AIHWIDGLLPVEEQVEIISINIRKFN"
     gene            1293..2948
                     /locus_tag="PLI16_05800"
     CDS             1293..2948
                     /locus_tag="PLI16_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013671760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH84109.1"
                     /translation="MTEKIQKLMRDVPSIRWTALVLLAFAMFCSYIFMDILSPVKDLL
                     ESTRGWDSTAFGTYAGSETFLNVFIFFLIFAGIILDKMGVRFTAILSGFVMLVGAAIN
                     WYAVTEMFMGSNLETWFTNNLNYIPGFDELGVSPFYRGMPASAKFAAVGFMVFGCGVE
                     MAGITVSRGIVKWFRGKEMALAMGSEMALARLGVATCMIFSPVIARSFGSVDVSRSVA
                     FGVILLMIALIMFIAYFFMDKKLDAQTGEAEEKDDPFRIRDIGKILSSTGFWLVALLC
                     VLYYSAIFPFQKYAVNMLQCNLTFTEIPADSFWASNSVTVVQYLIMLIVAATAFMSNF
                     SKRNKQIVLLTISIITLLIFCYMAYKRQSAESIFAVFPLLAVGITPILGKYVDNKGKA
                     ASMLMLGSILLIVCHLTFAFILPMFKGSNIGGVIVAFITILILGSSFSLVPASLWPSV
                     PKLVDKKVIGSAYALIFLIQNIGLWLFPLLIGKILNASNPDIAQALIDKTMTPEVAAV
                     SYNYTNPLLMLALLGVAALILGLILKMVDKKKGYGLELPNIQK"
     gene            3018..3701
                     /gene="mqnB"
                     /locus_tag="PLI16_05805"
     CDS             3018..3701
                     /gene="mqnB"
                     /locus_tag="PLI16_05805"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOH84110.1"
                     /translation="MKILIVSATIAEIQPLLNSLGITVEKNKNYYHSRIASHEVSVLI
                     TGPGMVATAFHLGEINLQNFDFALNAGIAGSFTNDLAIGELVHITTDCFAELGADYGD
                     NFIPLCKLEFATDLPDAIFNEQGEIVNPTTCGNPKLREVKSVKGITVNTINGEAHRIE
                     KIKRIFAPDTESMEGAAFLYACIKKKTPCFQLRTISNYIEPRNLNRWNIPFAIANLNR
                     QLPEIISAF"
     gene            3882..4721
                     /locus_tag="PLI16_05810"
     CDS             3882..4721
                     /locus_tag="PLI16_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HOH84111.1"
                     /translation="MSYIIKLGFSPCPNDTFIFDALINNKIDTEGIDFICDAEDIEAL
                     NQRAFEEDLDMTKVSFHAFLHLADKYQLLSYGSAMGNNCGPLLIGRKNYSDEEINKLT
                     IAVPGKYTTGAFLLNFAYPEVSDVHEIIFNKIENKILKNIVDAGVIIHETRFTYQDKG
                     LVKLCDLGAFWEQKTGFPIPLGGIVVRRDMPAELKARLSRIMKSSVEYAFGHPESSKD
                     FIREHSVEIDEEVIRKHIELYVNDYTLYLGEKGREAITFLFEYASKNKIIERIPDNIF
                     FEE"
BASE COUNT         1376 a         1083 c          957 g         1526 t
ORIGIN      
        1 cagaacatat ttttcgggtt ccgtactgcc atcgcaaaaa tgagtatgtg tatggtaatt
       61 ggttgttatc atatcgggca aagataggct tttttcgctg ttaaacgttg cgtatactaa
      121 ttgaacttac gaatgttaat gctgatgatt tccacctgct cttcaaccgg caacaaccca
      181 tctatccaat ggatggcaat tcccctccgt tccatgcctc tgaaccaggt catttgccgc
      241 ttggcaaact gatgtatcgc tatgttcaac aggcgaacca tttcgtcata cgaaaattcg
      301 cgcaacaaat attgtgtaat gtacctgtac tcaagcccat aacgaatcag ccgatcagcc
      361 ccgacacccg catccagtaa gcgtttaact tcgtctatca tgccttcttc caggcgttta
      421 tgcagccggt cggtgattcg catgcgcacc atatcacgcg gcatggagat accaaacaca
      481 taactatccc ccgcaaacaa tttttcagga agaagttttt tttcggcagc gaaatgggct
      541 atttccaatg cccttatcaa gcggcggcgg ttttcggtat cagtttcatt gtgcagtgtt
      601 ttgtacgact ccaaaagcgt ctgtagttcc ttatcggttt tgttctccag cgttttgcgg
      661 aaggcttcgt ttaccggcac atcgggaact gcataatttt tcagcaccgc ttcaatatac
      721 atgcctgttc caccgcaaag aaccggcgtt ttttttctca ggatgatctc gtcaaaaact
      781 tttttaaaat cggaaacaaa acgaaacaga ttgtactcct ctcctgcatc agcaacatca
      841 atcaggtgaa aggggatttt tttcccgtca accagataat catccagatc ttttccggtt
      901 ccaatggtca ttccccggta aacctgacgc gaatcagcgc tgatgatttc cccgccgttt
      961 ttttcggcca ccgccacagc cagccgcgtt tttccgcagg ctgtcggtcc caaaatagtg
     1021 atcaggctgt tttggtttgt catcatgtct ttatcgtttg taaccgtcat aatatgatac
     1081 tttttccaaa ataattgacc aaaaaaaagc tgctttacag tacacgataa ttatgatatt
     1141 tttaatcagg cattcttttt taatgctttt taccggataa tcagccattt taaaaacaat
     1201 gaagcttttg aaaatcctga ttaaaaaaaa tttccgccga ttgtcattgc aaatataaaa
     1261 agaataaatt tgtggcttaa tttaaaaaaa ctatgactga aaaaattcaa aaattgatga
     1321 gagatgtccc ttctattagg tggacagcac tggtattatt ggcatttgct atgttttgtt
     1381 cgtatatctt tatggatata ctttcgccgg ttaaagattt gctggaatca acccgtggct
     1441 gggattccac ggcttttggc acatatgccg gatctgaaac atttttaaat gtttttatct
     1501 ttttcctgat tttcgcaggt attattctgg ataaaatggg cgttagattt acggcaattt
     1561 tatcaggatt tgtaatgctt gttggcgctg caattaattg gtacgctgta actgaaatgt
     1621 ttatgggttc taatttagaa acctggttta ccaataactt aaattatatt ccgggatttg
     1681 atgaattagg tgtttctcct ttttacagag ggatgcctgc ttcggccaaa tttgccgcag
     1741 taggctttat ggtttttgga tgcggtgttg aaatggccgg catcacggtt tcacgcggta
     1801 ttgttaaatg gttcaggggc aaagaaatgg cgttggcaat gggatcagaa atggctttag
     1861 cccgtcttgg tgtggctacc tgcatgattt tctctccggt tatcgcacgt tcctttggtt
     1921 cagttgatgt ttcccgttcg gtggctttcg gagtgattct attgatgatt gccctcatca
     1981 tgtttatagc gtatttcttc atggacaaaa aacttgatgc gcaaaccggc gaagcagaag
     2041 aaaaagacga tccgttccgg attcgcgata tcggcaaaat cctgtccagc accggtttct
     2101 ggcttgtagc tttgctctgt gttttgtatt attctgccat tttccctttc cagaaatatg
     2161 ccgttaatat gctgcaatgc aacctgacct ttactgaaat acccgcagac tcattttggg
     2221 catcaaattc ggtaacggtt gttcagtacc taattatgct tattgtggct gcaactgctt
     2281 tcatgagtaa tttctcaaag agaaacaaac aaattgtgct gctgacaatt tcaattatta
     2341 ctctccttat cttctgctat atggcataca aacgtcaatc tgccgaatca atctttgcgg
     2401 tattcccatt gttagctgta ggaattaccc caattctggg aaaatatgtg gataataaag
     2461 gtaaagcagc ctccatgctt atgctgggat ctatcctgct tattgtttgt catttaactt
     2521 ttgcatttat tttgccaatg ttcaagggta gtaacatagg tggcgttatc gttgctttta
     2581 tcaccatctt gattctggga tcctctttct ctttggtccc ggcatcgttg tggccaagcg
     2641 ttccaaaact tgttgataaa aaagttatcg gttctgctta tgcattgatt ttcctgatcc
     2701 aaaacatcgg cttatggctt ttcccgctgc tgattggcaa aatcttaaat gcatccaatc
     2761 ccgatattgc acaagcattg attgataaga ctatgactcc cgaagttgca gccgtttcgt
     2821 acaattacac caatccgctc ttaatgcttg ccctgcttgg cgttgcagca ttgattctgg
     2881 gattaatatt aaaaatggtt gataagaaaa aaggatacgg ccttgaatta ccaaacattc
     2941 aaaaataaac cataacaatg tttttttcag aaagttgctc taaaaacagg gcaactttct
     3001 ttttttatat cgttttcatg aaaattttaa tcgtttccgc cacaattgcc gaaatacaac
     3061 ccttactcaa cagcctcgga atcactgttg aaaaaaacaa aaactattat cattcccgca
     3121 ttgcttctca cgaggtcagc gtactgataa caggcccggg catggttgcc acggcctttc
     3181 atctgggcga aatcaatctg caaaatttcg attttgccct gaatgccggc atagccggaa
     3241 gctttaccaa tgacctggcc atcggagaac tggttcatat taccaccgat tgttttgcag
     3301 aacttggcgc cgattacggg gacaacttta ttccgctttg caaactggaa tttgctactg
     3361 atttgccgga cgcgatattt aacgaacaag gcgaaatcgt aaaccctacc acctgcggga
     3421 atcccaaact ccgtgaagtg aaatctgtaa aaggaatcac cgtgaatacc attaacggcg
     3481 aggcccatcg catcgaaaaa ataaaacgca tctttgcccc cgacaccgaa agtatggaag
     3541 gagcggcctt tttgtacgct tgtatcaaaa agaaaacacc ctgttttcag ttacgtacca
     3601 tttccaatta catcgagccg cgcaacctca accgatggaa tatccctttc gccatagcca
     3661 acctgaaccg gcaattaccg gaaattatca gcgcattcta aaaatttttt taatcttatt
     3721 cgactaatga actttttagc aatacttttg cagagaaatt cagaattcag aattcagaat
     3781 tcagaattag gggtatcggt ttcaggcttc ccttctgact tctgatttct gacttttgac
     3841 ttctaacttt ttacttctaa cttctaaaat cctccttccc catgtcttac atcataaagc
     3901 tcggcttttc accctgtcct aacgatactt ttatttttga cgcccttatc aataataaaa
     3961 ttgataccga aggcattgat tttatctgcg atgccgaaga tatagaagcg ctgaaccagc
     4021 gtgcttttga agaggatctg gacatgacca aggttagttt tcatgctttt ttacaccttg
     4081 ccgacaaata tcaactgctc agttacggta gcgccatggg caacaactgc gggcccctgc
     4141 tgataggccg caaaaactac tccgacgaag aaatcaacaa actcaccatc gccgtacccg
     4201 gaaaatacac cacaggggct tttctgttaa actttgccta ccccgaagtg tcggatgttc
     4261 atgaaataat tttcaataaa attgaaaaca aaatcttgaa aaacatcgtt gatgccggag
     4321 ttattattca tgaaacacgg tttacttacc aggataaagg actggtgaaa ctttgcgatc
     4381 tgggtgcttt ttgggaacaa aaaaccggat tccccatacc gctcggcggt attgtcgtcc
     4441 gcagggatat gcccgccgaa ctaaaggcca ggctcagccg gataatgaaa tccagcgtag
     4501 aatatgcctt cgggcatccc gaatcatcaa aagattttat cagagaacac tctgttgaaa
     4561 tagatgagga ggtcatcaga aaacatatag aactgtatgt gaacgactac accttatacc
     4621 tcggggaaaa aggccgtgaa gccataactt ttttgtttga atacgcgagc aaaaataaaa
     4681 tcatcgaaag aattcctgac aatatattct ttgaagaata agacttattg aaaattattc
     4741 ccgtttcgca acaaatttcg accgcttctg ttttcccata aacccaattt aagcaataga
     4801 attgtttaat gcgtaattga ttccaactta attaaactaa ctgtatttca ataaattgta
     4861 agtttaataa gatatcggca tttatcaaaa cattttattt tttcgtagaa aaaaagtgcg
     4921 ttggcctgcc cagacggcgg gc
//
LOCUS       DAOSFP010000160         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15268_length_4927_cov_3.441092, whole genome shotgun sequence.
ACCESSION   DAOSFP010000160 DAOSFP010000000
VERSION     DAOSFP010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15268_length_4927_cov_3.441092"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..640)
                     /locus_tag="PLI16_05815"
     CDS             complement(56..640)
                     /locus_tag="PLI16_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84112.1"
                     /translation="MVRKTFFAIALIIGLQSILQAQDFYDRSGLLKANLTFNQGFMLF
                     HKAPNVYLGGNLEYFPDQNISIRGDCFWYLDSRRENPVLKQSTLVFFGALFHLPKGKS
                     DFYTGIQPGFAFTRPALLSSTDQSYPLRMMPSLALSLGYSFYFSRFCNFFVGVNYFVL
                     RYRGAGNGSLKLDELMISGGLGFHLSTKKQKPAN"
     gene            complement(634..1251)
                     /locus_tag="PLI16_05820"
     CDS             complement(634..1251)
                     /locus_tag="PLI16_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH84113.1"
                     /translation="MRTISNLLALLVFVSAQAQPLKQKNTPAGIFSLGARTCIGLVND
                     GQWQRTAFGTGGQFRLQFSDRVNTEWFADHLKADLSDYAWRADTHIGWSVMYYLTKKP
                     QPLVRPYVLAGHCFEYLKFTDNNDGSNFAERWSASVQGGAGAQLNISPRLDLSLAAQY
                     MLHLGTKIKPANTEGVTTFSKSEGLGIHDHILLHLSINYKIADLW"
     gene            complement(1531..1749)
                     /locus_tag="PLI16_05825"
     CDS             complement(1531..1749)
                     /locus_tag="PLI16_05825"
                     /inference="COORDINATES: protein motif:HMM:NF019749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOH84114.1"
                     /translation="MKKILFIVPAIAFLAGCQKEYQCTQQITKHVNGVEIDNQQIVMY
                     SENNLDGTCFNYEQTINDTVTVSKTTCE"
     gene            complement(2068..2286)
                     /locus_tag="PLI16_05830"
     CDS             complement(2068..2286)
                     /locus_tag="PLI16_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84115.1"
                     /translation="MKKFLLIVPLVGFLAGCQKEYQCTQQITKHVNGVEIDNQQIVMY
                     SENNLDGTCFNYEQTVNDTVTISKTTCE"
     gene            complement(2464..>4927)
                     /locus_tag="PLI16_05835"
     CDS             complement(2464..>4927)
                     /locus_tag="PLI16_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOH84116.1"
                     /translation="GSTRDHLISVKCLLSDGSEAEFGPLSRAEFTEKCSGDTLENRIY
                     RKLNEMLSRKALAAEIQENFPEKSIRRRNMGYALDELLDTEIFTEGSSSVFNMAKLIC
                     GSEGTLAFITEARLQLIPLPKQKSALLCVHLNTLEQAFRANLVALQHQPTAIELIDEV
                     IISLTKGNLSQQQNRFFIQGEPAAILIIELAHEDEVVLHEMAEKLVDELKSHGLGFHF
                     PLITGKDIARVRNLRKAGLGVLSSVTAAMKPHSFVEDTAVAPGQLGLYMAEFRAMIDS
                     YGVKCAFYGHIATGELHLKPILNLRTASGMELLQKISYDTAILVKKYRGSLSGEHGDG
                     RLRAGFIPLVLGNTVYQCLREVKNVFDPQHIFNRGKIIDAPCIAQDLREQRLLHEPQI
                     ATFFDYAKTNGLLHAIVRCNGSADCRKSHIIGGLMCPSFMASHEEKDSTRARANLLRE
                     ILADEKLSKPFASRELYDILDLCLMCKGCKAECPSGIDMARYKAEFLQHYYLAHHAAL
                     RTRLVAGITRINRIGACFPAVFNFFATNKYFSSALKRFLGFARQRQIPLLGKSTLKKW
                     AKKYLRQNNGLGEKILFFTDEFTNYTDVEIGKKALMLLVSLGYHVEIAPVSESGRTWF
                     SKGMLRQAKKISRKNIKKLSAVVSEQAPLVGIEPSAILSFRDEIPDIVGEDLRKNAEV
                     VAGCSFTIEEFIVKEYEAGTINAGLFTSEVKKIKLHGHCHQKALSTTLPTRKMLSIPI
                     NFLVEEIPSGCCGMAGSFGFEKEHYDLSMKIGGMVLFPALKKTGDDYIIVAPGTSCRQ
                     QIKDGTGKTAFHPVEVLFSSLV"
BASE COUNT         1431 a         1072 c         1001 g         1423 t
ORIGIN      
        1 acccaccaag aaataggaaa gccagtcctg acgttatgaa aaaccggcaa ctgctttagt
       61 tcgccggttt ttgttttttg gtactcaaat gaaaacccag cccacccgaa atcatcagtt
      121 catcgagttt cagggaacca tttccggcac cgcggtagcg caacacaaaa taatttacac
      181 ccacaaaaaa attgcagaac cgcgaaaaat aaaaactata tcccagcgac agcgccaaag
      241 atggcatcat gcgcagtgga taactctgat ctgtcgatga caacagagca ggccgggtaa
      301 aagcaaaacc cggctgtatt ccggtataga agtcgctttt ccctttgggt aaatgaaaca
      361 aggcgccaaa aaacacaaga gtactttgtt ttaacaccgg attctcccgg cggctatcca
      421 gataccagaa acagtcacca cggatgctga tgttttggtc aggaaaatat tcaaggttgc
      481 cgcccaggta aacatttggg gctttatgaa acaacataaa accctgattg aaagtaagat
      541 tagcttttag caaaccgctc ctgtcataaa aatcctgtgc ctgtaaaatg ctttgaaggc
      601 ctataatcaa agctatcgca aaaaatgttt ttcttaccat aggtcagcaa ttttatagtt
      661 aatactcagg tgcaacaaaa tatggtcgtg aatgcccagc ccttcagatt ttgaaaatgt
      721 agtcacccct tcggtgtttg ccggtttgat ttttgtcccc agatgcagca tatattgtgc
      781 ggccagcgat aaatccaacc tcggggaaat atttaactgt gcgcctgccc caccctgtac
      841 cgatgcactc cagcgttcgg caaaattgga accgtcgtta ttatctgtaa atttcaggta
      901 ttcaaaacaa tgtccggcaa ggacgtaagg cctgactaaa ggttgtggct tttttgtcag
      961 gtaatacatg acgctccagc ctatatgagt gtcggcccgc caggcataat cactcaaatc
     1021 ggccttcagg tggtcggcaa accattcggt atttaccctg tcggaaaact gtaggcgaaa
     1081 ttggcccccg gtgccaaacg cggtacgctg ccattgtcca tcgtttacca agccgatgca
     1141 tgttcgtgcg cctaaagaaa aaatgcctgc gggggtgttt ttttgtttta acggctgtgc
     1201 ctgggcagaa acaaaaacca ggagggccaa tagattgctg attgtcctca tagttattat
     1261 cttaggggtt aagataataa cgtgccgcgc cgcaacaaaa ttgcagaaat agaaaataac
     1321 tgtatatcag cagaaaactt tatggaaaag ttatacagag ctttttccct gtaaggattt
     1381 ttaaggctat taccagcctg gtagattttt ttgcgaagag aaataacaac agactacaag
     1441 ttaaaatcag catgtaatat tattgttttt gaaattgtta aaggatttta gccaatgtga
     1501 tcaataaatc gctggaatta ttaagcgttt ttattcacaa gttgtcttac tcaccgtgac
     1561 ggtatcattg atagtctgct cgtaattgaa gcaggtaccg tcgaggttgt tttcagagta
     1621 cataacaatc tgctgattat caatctcaac tccatttaca tgcttggtaa tttgttgagt
     1681 acactgatac tctttctggc aaccggccag aaacgcaatt gcaggtacga taaaaaggat
     1741 ttttttcatg tttcgtcttg atttttttat cattttcata tttattctat tgatatacgt
     1801 ataaatttat ggtataaaat gttttaaata aatattgtaa ttgataaatc cgcagacaaa
     1861 attactaaaa taaattgaga aaaagaaatt tttttcaaaa aaatataatt tattttcgga
     1921 taaaataaca aaaaaggcat ataaaaaaag cacttcagaa tatgaagcgc ttttatcacg
     1981 ggtaccttcc catttcaaaa ctaaggtctg cgtaccatgt aaagactaaa aattctataa
     2041 ataagaatga aagaaggtca atgacaatta ctcacaagtg gttttactta tcgtaactgt
     2101 gtcattaact gtttgttcgt aattaaaaca agtgccatcg aggttgtttt cagagtacat
     2161 aacgatctgc tgattgtcaa tctcaactcc atttacatgc ttggtaattt gttgagtaca
     2221 ctgatactct ttctggcaac cggccagaaa accaactaag ggcacaatta acaggaattt
     2281 tttcatgttt tatctggttt tttttctttg tttcatacac atataattga tatacgatta
     2341 atttttgtgt accaattttt gcttttaaat cttataccgt tataatttgt gtacgaattt
     2401 actaaaaaaa tttgcaatat ccaaattttt ggaaaaaaat ttttcaataa agagtaaaat
     2461 ttatcagacc aacgaagaaa aaagcacctc aaccggatga aaagccgttt ttcctgtacc
     2521 atctttgatc tgttgacgac aactggtacc cggagcaaca ataatgtaat catccccggt
     2581 ttttttcagg gcgggaaata aaaccatgcc tccgattttc atggaaaggt catagtgttc
     2641 tttttcaaaa ccaaaagacc ccgccatacc gcaacagcct gaaggtattt cttcaacaag
     2701 aaaatttatg ggtatggaaa gcattttacg cgttggcagt gtggttgaca atgctttctg
     2761 atgacaatgg ccgtgcagtt ttatcttttt gacctcagaa gtaaacaagc cggcattaat
     2821 agttcccgcc tcatattctt ttacaataaa ctcctcaata gtaaaagaac aaccggccac
     2881 aacctcagca ttttttctaa ggtcctctcc tactatatca ggtatttcgt ccctgaaact
     2941 caaaattgca gaaggctcaa ttccaacaag gggagcctgc tcactgacaa cagcagagag
     3001 ttttttgatg ttttttcggg aaatcttttt tgcctgcctg agcatccctt tggaaaacca
     3061 ggtccggccg ctttcgctaa cgggagctat ttcaacatga taacccaggc ttaccagcaa
     3121 cataagcgct tttttaccta tttcaacatc agtataattt gtgaattcgt cggtaaaaaa
     3181 cagtattttt tccccaaggc cgttgttttg ccttaaatat ttttttgccc acttcttaag
     3241 agttgacttg cccagcaggg gtatctgcct ttgccgggcg aaacccaaaa atctttttag
     3301 tgctgatgaa aaatacttat tagtagcaaa aaaattgaat acggctggaa aacaagcccc
     3361 aatcctgtta atccgggtta taccagccac cagtctggta cgcaaagcgg catggtgtgc
     3421 gagataatag tgctgtaaaa attcagcttt atacctggcc atatcaattc ccgacgggca
     3481 ttccgccttg cagcctttgc acatcagaca caggtcgagg atatcgtaaa gctcccggct
     3541 ggcaaaaggc ttgctgagct tttcatcggc aagaatttcg cgtaacaggt tggcccgggc
     3601 acgtgtgctg tctttttctt cgtggctggc cataaatgac gggcacatca ggccaccgat
     3661 aatatggctt ttgcggcaat ctgccgaacc gttgcaacgc acaatggcat gcagcaagcc
     3721 attagttttg gcataatcga aaaaggttgc tatttggggt tcatgtagca gccgttgctc
     3781 tctgaggtct tgtgctatgc agggcgcatc gataatcttc cccctgttga agatatgttg
     3841 agggtcgaac acatttttta cttccctgag acactgataa acagtatttc ccaacaccaa
     3901 tggtatgaat cctgcccgga gacggccatc accgtgttca ccgcttaggg agccacggta
     3961 tttttttacc agtatggcgg tgtcgtatga aatcttctgc agaagctcca tcccactggc
     4021 agttctgaga ttcaagattg gttttaaatg cagctctccc gtagctatat gaccgtaaaa
     4081 agcacatttt accccatagc tgtcaatcat ggcacgaaat tcagccatat acaaaccaag
     4141 ttgaccgggg gctactgcgg tatcttccac aaaactatgc ggtttcatgg ccgcagttac
     4201 cgagcttaac acgcccaatc cggctttgcg caggttacgc accctggcta tatccttacc
     4261 ggtaatcagg ggaaaatgaa aaccaaggcc atggcttttc agttcatcaa ccagcttttc
     4321 cgccatctca tgcaatacaa cttcatcttc atgtgccagt tcaattatca ggatggcggc
     4381 aggttcgccc tgaataaaaa aacgattttg ttgctggctc aggttaccct ttgtcagcga
     4441 gataattacc tcatcgataa gttcgatggc tgtgggctga tgttgcagcg caaccaggtt
     4501 ggcccggaat gcctgttcaa gggtattcaa atgcacacaa agcagggctg acttttgttt
     4561 tgggagaggt atcagctgca atctggcttc agtaataaat gccagggtac cttccgaccc
     4621 gcagataagt ttagccatat tgaaaaccga tgagctgcct tcagtaaaaa tttctgtatc
     4681 aaggagttca tctaaagcgt aacccatatt tcgcctgcgg atactttttt cgggaaaatt
     4741 ttcctgaatt tcggctgcta aagctttccg gctgagcatt tcattcaact tccgatagat
     4801 gcggttttcc agtgtgtcac ctgaacattt ttcagtgaat tctgcgcggc tgagcggccc
     4861 gaactccgct tcgctgccgt cgctcaggag gcatttgaca gaaataagat ggtcgcgggt
     4921 gctgcca
//
LOCUS       DAOSFP010000161         4894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15402_length_4894_cov_3.050010, whole genome shotgun sequence.
ACCESSION   DAOSFP010000161 DAOSFP010000000
VERSION     DAOSFP010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15402_length_4894_cov_3.050010"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="PLI16_05840"
     CDS             <1..246
                     /locus_tag="PLI16_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84117.1"
                     /translation="IIVDPTVTLTNSLPYSVNEVGVYVNYILLDGSTWKTEKISFYNV
                     GPNRTVTKDAPPTERGTTLQAHICKVTSSSLGLYYSN"
     gene            269..751
                     /locus_tag="PLI16_05845"
     CDS             269..751
                     /locus_tag="PLI16_05845"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOH84118.1"
                     /translation="MEESRYLIFKSPIGNILIEERNKFITAISFIDQDREIRQSSAPL
                     LKICANQLQEYFTGKRKKFSIPFEQQGTDFQQQVWSEVGKIPFGNLRSYEKVAIKIKN
                     KKIVRAVGNAVGRNKLAIIIPCHRVIGKNGAMTGYAGGLWRKQWLIEHEQYYLLNLNQ
                     "
     gene            complement(765..1259)
                     /locus_tag="PLI16_05850"
     CDS             complement(765..1259)
                     /locus_tag="PLI16_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOH84119.1"
                     /translation="MKHLSEQTILELADGLLTPAEQSAAEAHITSCSACREQYDLFKS
                     LEILLSAENVRKAPPAITDRVMQQVELHNRIMARKAQSRKTLIKFTVIMSVFILALGV
                     LAFMSGAVITFQTPAWVTTIREFFDNLQIPKINPLYVFIILPVVFLLFAELVVNRISR
                     HRFA"
     gene            complement(1256..1810)
                     /locus_tag="PLI16_05855"
     CDS             complement(1256..1810)
                     /locus_tag="PLI16_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH84120.1"
                     /translation="MNEEEKIIIDKCLEGDTVAFRKLVDLYRNFAFTLAVRITKNEED
                     AEEVTQDAFLKAYIALKNFKGESKFSTWFYKIVLNLAMTKVRRRKPAHQNIDDIHMVL
                     TNDDEPVRKADRTYYVEKAISTLPEDEAAIVTLFYMEDQSLSDISEMVNLSLSNTKVK
                     LFRARIKMAEVLKNELKENVYSLL"
     gene            2051..2614
                     /locus_tag="PLI16_05860"
     CDS             2051..2614
                     /locus_tag="PLI16_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84121.1"
                     /translation="METFFGVLVPITLFLGLFTTLVFLRKYENDERMAMIEKGLEPVN
                     KKKTDRFGTLRFALLSIGVGIGIVLSIIIVQLIPKEPHQVTKTTSPEKLKVILNNLPV
                     KTTSDSSSKTLVITKDTVSESEEAVVEKEIDEELENMPTVTEEFTEYSRDGGLVGAIY
                     SGMILIFGGMGLLMAYRIRSKQKKLEE"
     gene            2817..>4894
                     /locus_tag="PLI16_05865"
     CDS             2817..>4894
                     /locus_tag="PLI16_05865"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH84122.1"
                     /translation="MEYRENEQLRLAAEFVQYTNKNIFITGRAGTGKTTFLHNLKKIT
                     PKRMVVVAPTGVAAINAGGVTIHSFFQLNFAPAIPEVLRAKKADASAEGYKHKFSRNK
                     IKLIKCIDLLVIDEISMVRADILDAVDEVLRRYRNRSKPFGGVQLLMIGDLHQLTPVI
                     KDDEWNILKEFYDSIYFFDSFALKKSQHVSIELKEIFRQTDKHFIGLLNKVRDNQTDT
                     ATIAEINTRHIPGFRAKDEDGYITLTTHNAGANSINQAKLDELKDVSQFFDADIEGEF
                     SPNDFPTDEKLELKVGSQVMFIKNDPSPEKLYYNGKIGKITRMEDNVVFVKCPSDNEE
                     IEVCKVAWENVRYSLNEDTREIDEKVIGTFSQIPLRLAWAITIHKSQGLTFDKVIIDA
                     HAAFAFGQVYVALSRCRTFEGIVLSTPISVAGIKTDTLVAEYTEDVRQNEPGPEKLRE
                     ARKLFQYDLIIELFDFNLLKYRFDELLKLTREHASVLEASKVIELEEKRRLFDAELFQ
                     VAEKFKQQLRYIMQQDVLPQENPALQERIRKASQYFSTNIKTKLSDYLNTIFFDTDNK
                     AIKKDLAESLEKLQKETFIKYSCIKNTHEGFDTLSYLRQRSDAEMDFRPPKYKAASQP
                     VASAQSKHPVLYQALKQWRNNLAEENNLPVYQILPQKTITELVNKLPTTLQALKTIKG
                     IGKVKSEIYGP"
BASE COUNT         1563 a         1068 c          986 g         1277 t
ORIGIN      
        1 atcattgtag atccgacagt tacccttacc aacagtctgc cctattccgt taatgaagtc
       61 ggggtatatg taaattatat tctgctggat ggctcaacgt ggaaaaccga aaaaatttcg
      121 ttttacaatg tcgggcccaa caggactgtt accaaagacg caccgccaac cgagaggggt
      181 acaacactac aggcacatat ctgcaaagtc acatcgagtt ctctgggatt atattattca
      241 aactagaagt ttcaccaatt ctacagtaat ggaagaatcc aggtatctga tatttaaatc
      301 acccattgga aatattctca ttgaagagag aaataaattt attacagcaa taagttttat
      361 tgatcaggac cgtgaaatca ggcaatcttc tgcacccttg ctgaaaatat gtgctaacca
      421 gctacaggag tactttacag gtaaaaggaa aaaattttcg attccttttg agcagcaggg
      481 taccgacttt caacagcagg tatggagcga agtgggtaaa attccgttcg ggaacctaag
      541 aagttatgaa aaagtagcta taaaaataaa aaacaaaaaa attgtccgtg ccgttggaaa
      601 tgcggttggc cggaacaaac ttgctataat cattccctgc caccgggtaa taggcaaaaa
      661 cggagccatg acaggctatg ccggcggcct gtggcgcaaa caatggctaa ttgaacatga
      721 gcaatattat ttgctaaacc tcaatcagta agacagttag tttttcaggc aaaccgatgc
      781 ctggaaatcc gattcaccac caattcggca aacagcagaa aaaccaccgg cagtatgatg
      841 aatacgtata gcggattgat tttgggaatt tgcaggttgt cgaaaaattc acgtatggtg
      901 gttacccaag ccggtgtctg aaaggtaatt accgctcccg acataaacgc cagtacgccc
      961 agagcaagaa taaacaccga cataatcact gtaaacttta tcagtgtttt tctgctttgc
     1021 gccttgcgtg ccataatgcg gttgtgaagc tccacctgct gcataaccct gtcggtgatg
     1081 gccggcgggg cttttcgcac gttttctgcc gacaatagta tttctaacga tttgaataaa
     1141 tcatactgct cgcggcatgc gctgcatgat gttatatgag cttcggctgc tgattgctct
     1201 gcaggcgtaa gcaagccgtc ggcaagctca aggatagtct gttctgataa gtgtttcata
     1261 acagcgaata tacgttttct tttaattcat tttttaaaac ttctgccatt tttatgcgcg
     1321 ccctgaacaa tttaactttg gtattggaca aactcaggtt aaccatttca gaaatatcgc
     1381 tcaaggactg gtcttccata taaaataagg taacaatggc ggcttcatct tcgggcagtg
     1441 tgctgatagc tttttccaca taataagtac gatcggcctt gcgcacaggt tcgtcatcat
     1501 ttgtcaacac catatgaata tcgtcaatgt tctgatgtgc cggttttctg cgtcttactt
     1561 ttgtcatcgc caaatttaaa actattttat aaaaccatgt gctgaatttc gactctcctt
     1621 taaaattttt gagggcgata taggctttca aaaaggcatc ctgcgttact tcttcggcat
     1681 cttcttcgtt ttttgttatc ctcacggcca gagtaaacgc aaaatttcgg tataaatcca
     1741 ccaactttct gaaggcaaca gtatcccctt ccagacattt atcaattata attttttctt
     1801 cctcgttcat aacactaaac aaatagacgc caggtttaat gaaatggtta caaattttct
     1861 caacaattgt ttaaatttac aaaaaaaatg caatacgacg caaatatttc attaattttg
     1921 aaagaataca ttatcacaaa atatagaaaa tcaacgatgt acaagaaatc aaaaaaaatt
     1981 ttgcgtactt ttgtaaccgg aatgaagaac gtgtcgtcat attgacaaaa ccaattaaaa
     2041 cttacacaac atggaaacat tttttggagt attagtacca attacattat tcctcggact
     2101 tttcactacg ctggtctttt tgcgtaaata cgagaatgac gaacgtatgg caatgattga
     2161 aaaaggcctg gagccggtca ataaaaagaa aacagaccgt tttggcacgt tgcgttttgc
     2221 gctgctgtcc attggtgtcg gtatcggaat agtgttgtca attatcattg tgcagttgat
     2281 ccccaaagaa cctcatcagg taaccaagac cacttcacct gaaaagctga aagtaatttt
     2341 gaataactta cctgtgaaaa caacaagcga ttcgtcatca aaaacactgg tcatcaccaa
     2401 ggatactgtt agcgagtcgg aagaagcagt tgttgaaaag gaaatagatg aagagctgga
     2461 gaacatgccc accgttacgg aagaatttac tgaatattcg cgtgatggcg ggttggtggg
     2521 agccatttac agcggcatga tactcatctt cggcggcatg ggtttgctta tggcataccg
     2581 catacgcagc aaacaaaaaa aattagagga ataagcaagt aatttttttg gaaaaaccgg
     2641 gcgaaaacgt ccggtttttt ttattttatc agaaggccgg aatattattt tgccataatg
     2701 atttatttat tttttttaaa tataaacaaa ttattcccta cttttgcaat tgttagcaca
     2761 tacaattgtt ggcacgatta gagatcgcac aaccaattgc aacaaattaa tacataatgg
     2821 aataccgaga aaatgaacaa ctgagactcg ccgcagaatt tgttcaatat accaacaaaa
     2881 acatctttat caccggaaga gccggtaccg gcaaaaccac ttttttgcac aacctgaaaa
     2941 agatcacccc gaagcgcatg gttgttgttg cccccacggg tgttgccgcc atcaatgccg
     3001 gcggtgttac cattcattcc ttttttcaac tgaacttcgc ccctgctatc cctgaggtat
     3061 tgagagcaaa aaaagcagac gccagcgctg aaggatataa gcataaattc agccgtaaca
     3121 aaataaaact catcaagtgt atcgacctgc tggtaattga cgaaatcagc atggtacgcg
     3181 ccgatatcct cgatgccgtg gatgaagtat tgcgaaggta ccgcaacaga tcgaaaccct
     3241 ttggaggcgt gcaactgctg atgattggcg atctgcacca acttacaccg gttataaaag
     3301 acgatgagtg gaatatcctt aaagaatttt acgacagcat atattttttc gacagttttg
     3361 ctctaaaaaa atcgcaacat gtcagtatcg aactcaaaga aattttcagg caaacggata
     3421 aacattttat cggcctgctc aacaaagtac gcgacaacca aacagataca gccaccatcg
     3481 ccgaaatcaa tacccgccac atacccggtt tcagggccaa agacgaagat ggctatatca
     3541 cccttaccac ccataacgcc ggcgccaaca gcattaacca ggcaaagctc gatgaactga
     3601 aagatgtttc acagtttttt gatgccgaca ttgaagggga attttcaccc aatgattttc
     3661 ccaccgacga aaagctcgaa ctgaaagtcg gctcgcaggt gatgttcata aaaaacgacc
     3721 cgtcgccgga aaagctttac tacaacggga aaatcggaaa aataacccgt atggaagaca
     3781 atgtggtttt tgtaaaatgc ccgtcggata acgaggagat agaggtatgc aaagtagcat
     3841 gggaaaacgt aagatactcc cttaacgaag atacccgcga gattgacgaa aaagtgatag
     3901 gcactttttc gcaaataccg ctgcggctgg cctgggccat caccatacac aagagccagg
     3961 gcctcacttt cgacaaggtg attatcgatg cccatgccgc ctttgccttt ggccaggtat
     4021 atgtggcgct gagccgctgc cgcacttttg aaggtattgt gttaagtacg cccatctcgg
     4081 tggccggcat caaaaccgat acactggtgg ctgaatacac cgaagatgtg aggcaaaacg
     4141 agcctggacc cgaaaaactc cgggaagccc ggaaactctt tcagtacgac ctgatcattg
     4201 aactttttga ttttaacctg ctaaaatacc gctttgacga actgttgaaa cttacccgcg
     4261 aacatgccag tgtgcttgaa gcttccaaag taattgaact ggaagaaaaa agaagacttt
     4321 ttgacgccga actatttcag gtagctgaaa aatttaaaca acaactgcgg tacatcatgc
     4381 agcaagacgt cctgccgcag gaaaatcctg ccctgcagga gcgtatcagg aaagcttcgc
     4441 agtatttttc taccaatata aaaacaaaac tctctgatta tctgaatact atctttttcg
     4501 acaccgataa caaggccatc aaaaaagacc tggctgaatc attggaaaaa ttacaaaaag
     4561 agacatttat caaatactcc tgtattaaaa atacccatga aggctttgat acactctctt
     4621 atttacgtca aagaagcgat gcagaaatgg atttcaggcc gccaaaatat aaagctgcct
     4681 ctcagccggt cgcttccgca caaagtaaac accctgtcct gtatcaggca ttaaaacaat
     4741 ggcgtaataa ccttgccgaa gaaaataacc ttccggtgta tcagatactg ccacaaaaaa
     4801 cgatcaccga gctggtcaat aaactcccca ccaccctgca agcgctgaaa accataaaag
     4861 gtatcggtaa agtaaaatca gaaatttatg gccc
//
LOCUS       DAOSFP010000162         4859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15545_length_4859_cov_3.670691, whole genome shotgun sequence.
ACCESSION   DAOSFP010000162 DAOSFP010000000
VERSION     DAOSFP010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15545_length_4859_cov_3.670691"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2139)
                     /locus_tag="PLI16_05870"
     CDS             complement(<1..2139)
                     /locus_tag="PLI16_05870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH84123.1"
                     /translation="MKKGKIKEFFATSWAIDNKSSIYVLVALITILGIFNYRSMPKEQ
                     LPDVVIPTIMVNTIYPGTSPQDMENLVTRPIEKQLKAVSNVKNVSSTSIQDFSSIIIE
                     FNTNIDQAEAKQKVRDALDKAKSSLPNDLYKDPEVIQIDFAEMPIMNIQISGNYDLER
                     LKKFAEMAQDRIESLKEITRVDIVGALDREIQVNIDMYKMQVAGISMYDVEKAISYEN
                     HTISAGEISMDGMKRNVRITGEFKDIDDIKNIVMVSTIGGKIYLKDIAEVKDGFKEQE
                     SFSRLNGQNVITLSVIKRGGENLIEASDKITEIVEDLKKNDYPSDLKVTSTGDISKYT
                     RSTLGELNNTIIFGFILIMFLLMFFMGFTNAFFVGISVPLSVFVSCLIFPGIDFSMNM
                     IVMFGLVFALGIIVDDAIVVIENTHRHHRDEPDIKRAAKGAAGEVFVPILSGTLTTLA
                     PFFPLIFWPGIVGEFMYFLPVTLVIVLLASLFVAYIINPVFAVTFMKHEFDVPITPIN
                     KKKTIISGCITVVLATIGYLLHWTGLANFIIFAFLMLLFYDFVLKRAIKKFREKTWPW
                     VIEKYEKLLRAVLLRKRSYYVVAGTVVLLLLTLVVMALWPPKIVFFADNEPNTISVSI
                     TLPVGTDQKITDSVTKIVEKRIGEAIGKDNPIVESVIANVTNGVAGDNFDFDRFPSPN
                     KGKVTVNFVEFSHRNGEKTNPYVEKIRDAVK"
     gene            complement(2163..3266)
                     /locus_tag="PLI16_05875"
     CDS             complement(2163..3266)
                     /locus_tag="PLI16_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOH84124.1"
                     /translation="MKRTILLVSLIIFVASCNSGDKTARLEKLKKQRDKITIEIQELE
                     KLISTESGKKVVPVAVTEIKPETFRHYLEVQGKVDGDDNVSVSAKNVGVITSINVKEG
                     QRVSKGQLLATLDAQILYTTLRDMETQLEFVNDLYIRQKNLWDQKIGSEVQYLTAKNN
                     KESLENKIQAMKDQIALSTITSPIDGTVEEISLKVGQAISPGYPTFRVVNFDHLKIVA
                     EVAEAHSAKVKQGDSVRIYFPDFDEEIDSKLSFTSKYINMLNRTFAVEAAIPNIHGRE
                     LRANMIAVIKINDYAAPSAIAISANLIHKSMNEYYVFVAEQVNNEMLAQKRVVTTGVT
                     YNGKTEILSGLQSGDNLITTGYNNLNGGEKIKF"
     gene            complement(3287..4609)
                     /locus_tag="PLI16_05880"
     CDS             complement(3287..4609)
                     /locus_tag="PLI16_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH84125.1"
                     /translation="MESFSLQEAMDYAVTNSYKVREASIDLQSTIARRKGYLSLGMPQ
                     INANASYQYHFNLPVQLMPNILTPAVDGSLVQHGLITPDQMMPVSDEKLPVQFGSTNN
                     LTVGVNASQVVFDGTYLVGLKAASMLVELSKASVDKSIREIKSSVAQAYYLVLIAREN
                     RRILDSTYTKMNVILEQTRQFHTSGFIEDTDVEQLSLMVSNLKNKMDMTDRNIDLAVD
                     LLQLQMGMDIDQEIILKDDLMTLLDQAIAYNLADKQFDVNDHIDFQLLKSQERLLSQV
                     VKVDKSAHYPTMALFFNTQSAAMREKFDFFDDGRWYNTTLVGVNLSIPIWSSGVRHYK
                     VMQDKFALEKHRITLKEAEDGLKIDINNSKSLLKTYSDQFYTDKKNYDLAVMIYDKTY
                     SKFKEGLASGMDLNQAYLQILSQHGTYLNTMMQLLNTYANLSKALNTL"
BASE COUNT         1261 a         1102 c         1044 g         1452 t
ORIGIN      
        1 tttaacagcg tccctgattt tttccacata gggattggtt ttttcaccat tacggtggct
       61 gaactccaca aagttgacgg ttacctttcc tttgttggga gaagggaaac ggtcgaaatc
      121 aaaattgtcg ccggcgactc cgttggtaac attggcaatg accgattcca cgatgggatt
      181 gtcctttccg atggcttccc ctatacgctt ttctacaatt tttgtaaccg aatcggtgat
      241 tttttgatcg gttccgacag gcaatgtaat tgacacgctg atggtgttgg gttcgttatc
      301 ggcaaaaaac acaattttgg gcggccataa tgccatgaca acaagcgtaa ggagcaacaa
      361 aacaaccgtg ccggccacaa cataatagga ccttttgcgc aataagactg cgcgcaataa
      421 tttttcatac ttctcaataa cccatggcca ggttttctcc ctgaactttt ttatggcccg
      481 tttgagcaca aaatcataaa acaacaacat cagaaaggca aaaataataa agttggccag
      541 tcctgtccag tgtaacaggt aacctattgt cgccagcacc acagtaatac agcccgagat
      601 aatggttttc tttttgttta tgggtgttat cgggacatca aactcatgtt tcataaacgt
      661 gacggcaaaa accgggttta tgatataagc tacaaaaagc gaagccagca gcacaattac
      721 cagagttacc ggcaaaaaat acatgaactc gccaactatt cccggccaga aaattaatgg
      781 aaagaaaggg gccaatgtag tgagcgttcc agataagatg ggcacaaaca cctcgccggc
      841 cgcccctttg gcggctcttt tgatatcagg ctcatcgcgg tggtgccggt gcgtattttc
      901 aataaccaca atggcatcat ctacgataat acccagcgca aataccagcc cgaacatcac
      961 tatcatgttc atcgaaaaat caatacccgg aaatatcagg cacgacacaa aaaccgacaa
     1021 gggcaccgaa ataccgacaa aaaacgcatt ggtaaaaccc atgaaaaaca tcagtaaaaa
     1081 catgatcagg ataaatccga agataatcgt gttgttaagt tctccaaggg tgctgcgggt
     1141 atatttggaa atatctcccg tagaggttac tttcaggtcg gatggatagt catttttttt
     1201 gaggtcttcc actatttcag tgatcttatc ggaggcttcg ataaggtttt ctccgcctcg
     1261 ttttatcaca ctcagggtga taacattctg accgttgagc cgcgaaaaac tttcctgttc
     1321 tttgaatccg tcctttactt cggcaatatc tttgagataa attttaccac cgatagtaga
     1381 caccataacg atatttttaa tatcatcaat atctttgaat tcgccggtga tacgtacatt
     1441 gcgcttcatg ccgtccatgc tgatttcgcc ggccgagatc gtatgattct cgtatgaaat
     1501 agccttttcc acatcgtaca tcgaaatgcc ggccacctgc atcttataca tgtcgatatt
     1561 aacctgaatc tcgcggtcga gtgctcccac aatatccacg cgggttattt ctttcagcga
     1621 ctctatcctg tcctgggcca tttcagcaaa ttttttcaaa cgttccaggt cgtaattgcc
     1681 cgatatctgt atgttcatga tgggcatttc agcaaaatca atctgtatca cttcaggatc
     1741 cttataaagg tcgttgggca acgacgattt tgctttatca agggcgtcgc gtaccttttg
     1801 tttggcttcc gcctgatcga tattggtatt gaattctatg atgatgctcg aaaaatcctg
     1861 tatggaggta ctgctgacat ttttgacgtt ggataccgct ttcaactgtt tttctatcgg
     1921 gcgggtaacc aggttttcca tatcctgcgg agaagttccg ggataaatag tgttgaccat
     1981 gatggtggga atcaccacat caggaagctg ctccttgggc attgaccggt aattaaaaat
     2041 tccgagtatc gtgatcaggg caaccaatac atagatactg gatttgttgt cgatagccca
     2101 gcttgtcgcg aaaaactctt ttattttgcc ttttttcatt tccaaagtat taatcctaat
     2161 aatcagaatt ttattttttc gccgccgttc aggttgttat atccggtagt aataagatta
     2221 tcgccggatt gcagcccgct gagaatttct gttttaccat tgtaggtaac accggtggtt
     2281 accacgcgtt tttgggccag catctcgtta ttcacctgtt cagccacaaa aacatagtac
     2341 tcattcatgg atttgtgaat caggttggcc gaaatcgcta tggcactggg tgcggcataa
     2401 tcattaatct taatcacggc aatcatattg gcccgtagtt ctcgtccgtg gatgttaggt
     2461 atggctgcct ccacagcaaa agtacggttt agcatattga tgtacttgct ggtaaaacta
     2521 agtttcgagt caatttcctc gtcaaaatcc gggaaataaa ttcttacgct gtcgccttgt
     2581 tttacttttg cagaatgggc ttcggccacc tcggccacga ttttgaggtg atcgaaattc
     2641 acaaccctga atgtgggata acccggcgaa atagcctgcc ctaccttcag cgaaatctcc
     2701 tccactgtgc catcaatagg cgatgtaatg gtggacaggg caatctggtc tttcatggcc
     2761 tgaatcttgt tttcgaggct ttccttattg tttttggccg tcaggtactg cacttccgaa
     2821 ccaatcttct ggtcccaaag gtttttttgc cgtatataca aatcattgac aaactcaagc
     2881 tgggtctcca tatcgcgcag cgttgtgtac aatatctggg cgtcgagggt agcaagcagt
     2941 tgccccttgc ttacccgttg gccttctttt acattgatgc tggtaatcac accgacattt
     3001 ttggcgctga cagacacatt atcgtctccg tctaccttgc cctgcacctc caggtaatga
     3061 cggaaggttt cgggttttat ttcggtaaca gccaccggaa cgactttttt tcccgattcg
     3121 gtacttatca acttttcaag ctcctgtatc tcgatggtaa ttttatcgcg ttgttttttc
     3181 agtttctcaa gcctggcagt cttgtctccg ctattgcacg aggctacaaa tatgatcagg
     3241 cttaccaata aaattgttct tttcatagga tttctttttt ctgtatttac aatgtattta
     3301 aggctttact caggttcgca taggtgttca gcaactgcat catggtgttg aggtacgtgc
     3361 catgttgcga caatatctga aggtaggcct ggttgaggtc catacccgaa gccagcccct
     3421 ccttgaattt ggaataggtt ttatcataaa tcatcacggc caggtcgtag tttttcttat
     3481 cagtataaaa ctggtcgctg tatgttttca gaagtgactt ggaattgttg atatcgattt
     3541 tcagcccgtc ttccgcttct ttcagcgtga tgcggtgttt ttccagtgcg aatttatcct
     3601 gcatgacttt ataatgtctt actcccgagc tccatatagg tatggaaaga tttactccaa
     3661 ccagggtggt attataccag cggccatcgt caaaaaaatc gaacttttcg cgcatcgcgg
     3721 cagattgtgt attaaaaaat aaagccatag taggataatg ggccgattta tccactttaa
     3781 ccacctgact gaggaggcgt tcctggcttt tcaacaattg aaaatcaata tggtcgttca
     3841 catcaaactg tttatcggcc agattgtagg ctatagcctg atccagcaag gtcatcaaat
     3901 cgtctttcag tattatttcc tggtcaatat ccatacccat ctgcagttgc agtaaatcca
     3961 cagccaggtc gatatttctg tcggtcatgt ccattttgtt tttcaggttg gaaaccatta
     4021 gcgaaagttg ttccacatcg gtatcttcga tgaagccgct ggtgtgaaac tgccgtgtct
     4081 gttcgagtat cacattcatt ttggtgtagg tcgaatcaag gatgcggcgg ttttcgcggg
     4141 caataagcac caggtaatat gcctgtgcca cactggattt gatctcgcgg atggatttat
     4201 caacggaggc cttgctaagc tccaccagca tggaggcggc tttgaggccc actaaatagg
     4261 taccgtcaaa gacaacctgt gaagcattga ccccaaccgt gaggttgttg gttgatccaa
     4321 actgtaccgg cagtttctca tcgcttacgg gcatcatctg atcgggtgtt atcagcccgt
     4381 gctgcaccaa agagccgtca accgcgggtg ttaggatatt aggcatcagt tgcacaggga
     4441 gattgaaatg gtactgataa ctggcattgg cattgatctg tggcatgccc agagaaagat
     4501 aacctttgcg ccgggctatg gttgactgca aatcaataga agcctcacgc accttgtagc
     4561 tgttggtaac ggcataatcc atggcttcct gcaatgaaaa gctctccata gctgtggact
     4621 ggcacattgc ccgatcagca caacacaagc atacgacgta taaaacagta actttttgca
     4681 atattttttt aatcattcat ttagtatttg gtgtaagaca aatctttttt tcaaagtatt
     4741 ctatcccctt tttgctagca ataccgcgaa agtgataggt aaacatttcg ctgaaaagct
     4801 cattagtggt aaattccata cttgtaataa aacccgaacc cagcgaagct tcgatttgg
//
LOCUS       DAOSFP010000163         4858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15553_length_4858_cov_3.571310, whole genome shotgun sequence.
ACCESSION   DAOSFP010000163 DAOSFP010000000
VERSION     DAOSFP010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15553_length_4858_cov_3.571310"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..2092)
                     /locus_tag="PLI16_05885"
     CDS             complement(158..2092)
                     /locus_tag="PLI16_05885"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HOH84126.1"
                     /translation="MKKLVLVLMLIVPAFLLQAQIPVSTTPQNKNVILEEFTGIHCGF
                     CPQGHLVAHNIMTANPNRAYAIAVHTGSYATPGAGEPDYRTSFGTSLASQTALTGYPA
                     ATVNRHVFSGLGQTSGGTAMSRTNWTSASTTILGQPSYLNVGVAAQVNYTTRVLTVHC
                     QVYYTGNSSVTTNKLNIAILQNNVKGPQSGSSGNPTMVTPDGQYLHQHMLRHMLTGQW
                     GVSIPNTNTASGTLKVDTTITYTVPADYTGVPVNLAELEIIAFVAEGNQEIISGSGCT
                     VNQPTIDAAITSITGLPLIQCTTNPIAPTVVLKNKGTDVLTSAVINYSVDGGTTVSQN
                     WSGTLAAGATANVTITNPITPVNGHHTVRCFSSLPNGVADLNALNDDYSAAYNAFLNY
                     STSPVNEEFTSTAFPPTNWVIDGLHWSRQSVSSFGVGSGSARVYFYGAETGAINDMYI
                     YGLDLSGVSSYHLSFDHAYAQYQTGYDDRLQVQVSTNCGSTWTSIFDKAGSTLATAPA
                     SSSSFSPSASQWVTNNINLNSYVGQPNVIIRFHTISDYGNNLFIDKINTGTGTGVNES
                     YDDSNIQIFPNPASNVINIVNAENADIMLYDLLGKLVMSANNISNNFSINVSDFATGN
                     YIVKIIDEGNITTDKISVIK"
     gene            complement(2277..3467)
                     /gene="ygeW"
                     /locus_tag="PLI16_05890"
     CDS             complement(2277..3467)
                     /gene="ygeW"
                     /locus_tag="PLI16_05890"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF005538.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOH84127.1"
                     /translation="MKKILEIINEIKKNRSTLYGKDFLLTWDKTTEELNLVLLVAEAL
                     KTMRAENISPRVFDSGLAISNFRDNSTRTRFSFASACNLLGMAVQDLDEGKSQIAHGE
                     TVRETANMISFLSDVIGIRDDMFLGKGHTYMLEVAKALDEGFKKKVLPCRPAIVNLQC
                     DIDHPTQSMADLLHLKNYFGSIENLRGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MHVELAHPEGYDLIPDVMDLAFKNALESGGSFKVSKNMNTAFKNADIVYPKSWAPFSV
                     MQQRTKLLLANDTEGLKTLEKQCLANNAKHKDWECTRENMKLTKDGKALYMHCLPADI
                     SGVSCKEGEVEKQVFEKYRIPTYIEAGYKPYIISAMILTSLFKNIPGTLEKILDNNIE
                     RKQK"
     gene            complement(3479..4666)
                     /locus_tag="PLI16_05895"
     CDS             complement(3479..4666)
                     /locus_tag="PLI16_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOH84128.1"
                     /translation="MSISIEKVKELAEKYQDYTANNLSALIRAKSLSGEEKQVAEELS
                     RQMKEAGFDEVIIDGLGNVIGRIGNGKKILAIDGHIDTVDLGNMANWSFDPLSGLIKD
                     GFVHGRGTVDQKGGPAAFVTAGRIIKELGLDNDLTLYCVGSVMEEDCDGLCWKYIIEE
                     DKIVPHTVISTEPTNLQIYRGHRGRMEIEISFRGISAHGSAPERGKNAIYMASRVCLD
                     IEKLNENLAYDEFLGKGTVALAEFVSGSPSLCAVADYAKIHLDRRLTWGETKESAMAE
                     IQQIIKDTDAKAEVLYYNGKGYTGLNYGMEKYYPTWKIEENHNAVQTGVKAYQQLYDK
                     APVIDKWTFSTNGVTINGIYKIPVIGFGPGNEVLAHAPNEKVAVSDLVTASAFYSAYA
                     LLF"
BASE COUNT         1237 a          998 c         1144 g         1479 t
ORIGIN      
        1 ttaatagcta taggcgtttg ctgagttttt accattaatt ttgtccatta accctatttt
       61 aatgattaaa tatttttttt caaagataaa caataaaaaa aagagcaaaa aaaaactgca
      121 gtattttgct gcagtttttt ttaatgtgcg gtaattatta tttaattaca gatattttgt
      181 cggttgtgat attcccttca tcgataatct tgacaatata gttaccagtt gcaaaatccg
      241 aaacattaat gctgaaattg ttgctgatgt tatttgcact cataaccagt tttccgagta
      301 aatcataaag cataatatct gcattctcgg cattgacaat gtttattacg ttgctggccg
      361 gattggggaa tatctgaata ttgctgtcgt cataagattc gttaactcct gtaccggtgc
      421 cggtattaat tttatcaata aaaagattgt tgccataatc gctgattgtg tggaagcgaa
      481 tgataacgtt cggctgacca acataagagt tgaggttgat gttgttggtc acccattgtg
      541 aagcagatgg tgaaaacgat gaggaagatg cgggagcagt agccagtgtg cttcctgctt
      601 tatcgaagat gctcgtccat gtgcttccgc aattggtgga aacctgaact tgcagacgat
      661 catcatatcc tgtctggtat tgtgcatagg cgtggtcgaa agacaagtgg taggatgata
      721 ctcctgacaa gtcaagcccg tagatataca tgtcgttgat agcgcctgtc tctgctccat
      781 agaaatacac tctggccgaa cctgatccaa cgccgaaaga actgacggat tgtcttgacc
      841 aatgtaagcc gtcaatcacc caattggtag gagggaaggc ggtacttgta aattcttcgt
      901 ttaccgggct ggtactgtaa ttcagaaatg cattgtaagc tgcagaataa tcatcattga
      961 gtgcattgag gtcggccacc ccgttgggta aggaactaaa acatcttaca gtatgatgac
     1021 cgttaacggg ggtgatagga tttgttatgg taacgttggc agtagctcct gcagccagtg
     1081 ttcccgacca gttttgcgat acggtagtgc cgccatccac agaataattg attactgcgc
     1141 tggttaatac atcggttcct ttgtttttta atactacggt gggagcaatc ggattagttg
     1201 tacattgaat caggggcaat cccgtgatgg aagtgatagc ggcatcaatg gtcggttggt
     1261 ttacggtgca tccgcttcct gaaataattt cctgatttcc ttcagctaca aaagcgataa
     1321 tttccagttc ggcaaggttc acaggcacgc cggtgtaatc ggcaggcacg gtgtaggtga
     1381 tggtagtatc cactttcagc gttccggagg ctgtattggt attgggtata gaaactcccc
     1441 actgtcctgt taacatatgc cgcaacatgt gttgatgcag gtattgtccg tcaggagtta
     1501 ccatagtagg atttccgctt gaaccggatt gcgggccttt cacattgttt tgcaatatgg
     1561 cgatgtttaa tttgttggtt gttacagagc tgtttcctgt ataatatacc tggcaatgaa
     1621 ctgtgagcac tcttgtggta taattaacct gagccgcaac cccaacattt aaatacgaag
     1681 gttgtcccaa aatggtggtg ctggcgctgg tccaatttgt ccttgacata gctgttcccc
     1741 ccgatgtttg tcccagaccg gaaaaaacat ggcggtttac tgtagcggca gggtatcctg
     1801 ttaaagccgt ttgtgaagca agagatgtac caaaagaagt tctgtaatcg ggctcgccgg
     1861 cgccaggggt ggcatagctg ccggtgtgta cggctattgc ataagcacga ttgggattgg
     1921 ctgtcataat attatgagcc acaaggtgcc cctgagggca gaacccgcaa tgaataccgg
     1981 tgaactcttc cagaatcaca tttttgttct gcggggtggt gctgacaggt atctgagcct
     2041 gtaacaaaaa cgccggaaca atgagcatta atacaagtac taattttttc ataaagaatt
     2101 tttttggtta atagttaata aatgaaattg gcataacata caaagataaa ggaaaatctt
     2161 tgtacccccc tttatttaac ttataattaa ctaatacact ataccttatg aaggaaactc
     2221 aatgtcggtc aagatattga gtttgtagca atgttcccga tactattctg tagcgtttat
     2281 ttttgttttc tttcgatgtt attatcgaga attttttcaa gcgttccggg aatattttta
     2341 aacaggctgg taagtatcat agccgaaatg atgtaaggtt tataaccggc ttcgatgtat
     2401 gtggggatgc ggtatttttc aaagacttgt ttttctacct cgccttcttt gcatgaaacg
     2461 ccggagatgt cagccggcag gcagtgcata tacaaggctt tgccgtcttt ggtgagtttc
     2521 atattttcgc gcgtacattc ccagtctttg tgttttgcat tgttggcaag gcactgtttt
     2581 tccagtgttt ttaatccctc ggtgtcgttg gccagcagca gtttggtgcg ttgttgcatg
     2641 accgaaaaag gtgcccagct tttagggtat acgatgtcgg catttttaaa agcggtattc
     2701 atatttttcg acactttaaa gcttccgccg ctttccaggg catttttaaa ggcaaggtcc
     2761 atcacgtcgg gtatcaggtc gtagccttcg ggatgcgcca attcaacgtg cattccgaaa
     2821 cgtgtcatca gcccgatgat tccctgtgga acggataatg gttttccgta gctgggcgaa
     2881 taggcccagg tcattgcgat ttttttgccc cggaggtttt caatggatcc gaaatagttt
     2941 ttcaaatgga gcaggtcggc catggattgg gtggggtggt caatatcgca ttgcaggttt
     3001 acgatggcag ggcggcaggg gagtactttt ttcttaaatc cttcgtcgag ggctttggcc
     3061 acttcgagca tataagtatg tcctttgccc aggaacatat catcgcgtat gccgatgaca
     3121 tcagataaga aagaaatcat attggctgtt tcgcgcacgg tttcgccgtg ggcaatttgc
     3181 gacttgcctt cgtcgaggtc ctgaacagcc atgccaagca aattgcacgc agaggcaaag
     3241 gaaaaccggg tgcgggttga attgtccctg aagtttgaaa tggccaggcc tgagtcaaac
     3301 acccgcggag aaatattttc ggcacgcatg gttttcaatg cttcagcaac cagaagaaca
     3361 aggttcagtt cttcggtggt cttgtcccag gtaagtaaaa aatcttttcc gtataatgtg
     3421 gaacggtttt tctttatttc attaatgatt tctaaaattt ttttcatagt aattatgttt
     3481 aaaataataa tgcataagcg gaataaaaag cagaagcggt aaccaggtcg ctgacggcaa
     3541 ctttttcgtt gggggcatga gccagcactt cgttgccagg tccaaagccg atgacaggga
     3601 ttttataaat gccgttgata gttactccgt tggttgaaaa agtccatttg tcaattacgg
     3661 gcgccttatc atataattgt tgatatgcct taacaccggt ctgtacggcg ttgtggtttt
     3721 cttcaatttt ccatgtggga tagtattttt ccatgccata attcaggccg gtatagcctt
     3781 ttccgttgta ataaagcact tcggccttag catcggtatc tttgatgatt tgctggattt
     3841 cggccatggc agattctttg gtctcgcccc aggtaaggcg gcggtccaga tgtatctttg
     3901 cataatcggc cacagcgcat agtgaagggc tgccggaaac aaattcggcg agagccacag
     3961 tgcccttgcc aagaaactcg tcgtaagcca ggttttcgtt gagtttttct atgtcgagac
     4021 ataccctgga agccatgtag atggcgtttt ttccgcgttc gggagcagag ccgtgggcag
     4081 atattcctct gaaggaaatt tcaatttcca tacgtccccg gtgtcctctg taaatctgca
     4141 ggttggtagg ttcggtgctg attactgtat gaggaacaat cttgtcttct tcaattatat
     4201 atttccagca cagtccgtcg cagtcttctt ccatcacact gccgacgcag tataaggtaa
     4261 ggtcattatc gagtcccagc tctttaatta tcctgccggc agtaacaaag gcagcggggc
     4321 cgcctttctg gtccacggtg ccgcgtccat gtacaaaacc atcttttatt aagccgctca
     4381 gcgggtcgaa cgaccagttg gccatattac ccagatccac cgtgtcgatg tgtccatcga
     4441 tagcaagaat tttttttccg ttgccgatgc gcccgataac atttcccaga ccatcgataa
     4501 tcacttcgtc aaaaccggct tccttcatct gccggctaag ttcttctgct acctgttttt
     4561 cttctccgct gagggatttt gcccgaataa gcgctgatag attattggct gtataatcct
     4621 gatatttttc agccagttct tttacttttt caatagaaat actcatgatc ttgttatttt
     4681 tttggggaca aaaataacac aaaaattatg accagcccaa attaaaaaat gagctggtca
     4741 taaaagtttt aaaaataaga tgttctatca gtttttgtcg ggagaaatca tcttgtacac
     4801 gatacctgcg agcatggcgc ctactatggg agcaagccag aacagccaga gctggctc
//
LOCUS       DAOSFP010000164         4854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15575_length_4854_cov_3.646176, whole genome shotgun sequence.
ACCESSION   DAOSFP010000164 DAOSFP010000000
VERSION     DAOSFP010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15575_length_4854_cov_3.646176"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(369..3413)
                     /gene="infB"
                     /locus_tag="PLI16_05900"
     CDS             complement(369..3413)
                     /gene="infB"
                     /locus_tag="PLI16_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOH84129.1"
                     /translation="MEETVKTYRLSKIATEFNVSVNSIIDFLSKKGIKVDNNPNGKIT
                     SDIYEILQKEYSKDKQVKETSKKLDIGSVRHETITLDKAKDREKDTDLTQDKELFIKN
                     VPVSYPVESKIIEKKEEPAQKAAEPAKKAIKEEPQTVEPEKSSTEKPAKKAPKQVEEK
                     IKEVEKASEKASEKEEEETEETQKTGKLKGLKVVGKIDLEKVSEKKPGKKVAEETAKE
                     TKKSKAKTEVAEEKIEAPEEKKTTKKAKKTETPVEPEPEVKEEIAEKVVAEEPPVVTE
                     EIAPAKEEVKEDNNLIATEYIKLHGPKVLGKIELPVEKPPQKQPVATSSDNKVSDRKR
                     RRKRIKKEGIQPGESEMQGKTGGTGSGTGTSRTSRHDSRKSKEPLHPEVSEEEIQKQI
                     RDTLAKLTGPTKSKASKYRRLKRDMVQQQIEEEHLKEEEEKKILKVTEFVTVNEISTL
                     MNVPVTSIISTCMTLGLFVSINQRLDAEVLSIVADEFGFKVEFISAELEQAVHTEKDA
                     PETLLERHPIVTVMGHVDHGKTLLLDYIRNSNVIAGEAGGITQHIGAYEVTLEDGKKI
                     TFLDTPGHEAFTAMRARGAKVTDIAIIVIAADDSVMPQTVEAINHAQAAKVPLIFAIN
                     KIDKPTANPEKIKEALAAMNLLVEDWGGKYQCQEISAKKGINIDKLLDKILLEAEMLE
                     LKANPNRNAYGTVLESSLEKGRGYVAKLLMLNGTLHVGDMVLAGSSYGRVKAMYNERN
                     LTMETAGPATPLLMLGLNGAPQAGDTFTVMQDEREVKEIANKRFQLQREQNIRTHKHI
                     TLDEIGRRIAIGDFKELNIIVKGDVDGSVEALSDSLLKLSNKEVQVNVIHKSVGQITE
                     SDVLLASASNAIIIGFQVRPSLTARKHAEHEQIDIRLYSIIYNAINEINSAIEGMLSP
                     EIEEKIVCNLEVREVFKITKVGTIAGCQCLDGKITRNTLVRIIRNGIVVYSGKLGSLK
                     RFKDDVKEVFAGQECGLNIENFNDIKVEDIIEGYEQIEVKRK"
     gene            complement(3479..4714)
                     /gene="nusA"
                     /locus_tag="PLI16_05905"
     CDS             complement(3479..4714)
                     /gene="nusA"
                     /locus_tag="PLI16_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOH84130.1"
                     /translation="MEHINLVESFSEFKEYKNIERETMMRILEDVFRHMLIKKYGSDE
                     NFDIIVNIDKGDLEVWRNRKIVDDNEVTDPNQQIAYSDAIKIEPDFEIGEDVSEEVRL
                     KDFGRREILAMRQNLVSKIMEHEKDNIYKEYIEKVGQIVTGEIYQVWKKEILVLDDDG
                     IELIIPKSEQIPSDFYRKGESVRAVVSKVEMKNGTPVIILSRTSPVFLERLFEAEVPE
                     VFDGLITIKKIVRIPGERAKVAVESYDDRIDPVGACVGMKGARIHGIVRELRNENIDV
                     INFTTNISLYITRALSPAKVSSIKIDEKNKKADVFLKPDQVSLAIGKGGYNIKLAAKL
                     TGYEIDVYRDSDSDFEDVDLQEFADEIEEWIIEELKNVGCDTAKSVLELSVDELVKRT
                     DLEEETIREVIKILRSEFE"
BASE COUNT         1100 a          993 c         1025 g         1736 t
ORIGIN      
        1 tagtatgaaa aagttattaa caattacgta ttaataagaa gagttacggt ttaaaaaaaa
       61 tcagaaaagt aattttgttg attaattaaa atagaataaa gaaataaatt aaaaacaaac
      121 tggcgattgt ttattatgag ctggaggtat tttcgaaaag aagtttgtat ccctgagaat
      181 tatttgataa gaaactgaaa aaccttgcgg tatttatcag ctaataacat attgtatatt
      241 tttagctcaa gaaccaaaaa ctaaatacaa tgtctgccta ggccaatgaa atctgcctat
      301 aatcaataag acaaagtcga taagggctat ctgccagccg gccgatgaat gggcaaccgg
      361 aattatgctt acttgcgctt gacttctatt tgttcgtagc cttcaataat atcttcaact
      421 tttatatcgt tgaagttttc gatgtttaac ccgcattcct gacctgcaaa gacttctttt
      481 acgtcgtctt taaagcgttt cagggagcca agtttgccgg agtatactac gatgccattt
      541 cggatgatgc gcaccagggt gtttctggtg attttgccgt caaggcattg gcatcccgcg
      601 attgttccaa ctttggtaat tttaaatact tctctgactt cgaggttgca tacaattttt
      661 tcttcaattt ctggcgaaag cataccttca atggcggagt tgatttcgtt gatggcgtta
      721 tagatgatgg agtacagcct gatgtcaatt tgctcgtgct ctgcatgttt tctggcggtg
      781 agcgaaggac gtacctggaa tccaatgata attgcattgg aagccgaagc cagcagaacg
      841 tcagattccg taatctggcc aactgattta tggatgacat tgacttgtac ttctttgttg
      901 gaaagtttga gcagcgaatc agataaagct tccacagaac cgtccacatc accttttacg
      961 ataatattaa gttctttgaa atcaccaatt gcaatacgcc gtcctatttc gtcaagggta
     1021 atgtgtttgt gcgttctgat gttttgttcg cgctgcagtt ggaatctttt gttggctatt
     1081 tcctttactt cgcgttcgtc ctgcataacg gtaaaggtgt ctccggcctg cggggcgccg
     1141 ttaagtccaa gcatcaacag tggagtggca ggacctgctg tttccatggt caggttacgt
     1201 tcattataca tggctttaac tctgccatag ctggacccgg ccaaaaccat atcgccaacg
     1261 tgcagcgtgc cattaagcat caatagtttg gcaacataac cacggccttt ttccagcgag
     1321 gattccagta cggtgccgta ggcattgcga ttgggatttg ctttcagttc gagcatttca
     1381 gcttccagca gaatcttatc caacaacttg tcgatgttga tacctttttt tgctgatatt
     1441 tcctggcatt ggtattttcc gccccagtct tccaccagaa ggttcatggc cgccagggct
     1501 tcttttattt tttcgggatt ggccgtaggt ttatctatct tgttgatagc aaaaatcaag
     1561 ggaacttttg ctgcctgggc gtgattgatg gcttccacgg tttggggcat gacactgtcg
     1621 tcggcagcta ttacgatgat tgcaatatcg gtaactttag caccgcgggc acgcatggcg
     1681 gtaaaggctt catggccggg ggtgtcaagg aaggtgattt tctttccgtc ttccagcgta
     1741 acctcatagg ctccaatatg ttgagtgata cctcccgcct ccccggcaat gacattggag
     1801 ttgcggatat aatcgagcaa caaggttttc ccatgatcca catgtcccat aaccgtaaca
     1861 atagggtgac gttccaggag cgtttcggga gcatctttct cggtatgaac ggcctgttcc
     1921 agttcggcgc tgataaattc taccttgaac ccgaattcat cggcaacaat agataaaact
     1981 tctgcatcga ggcgctggtt aatggaaaca aacaaaccaa gcgtcataca ggtggagata
     2041 atgctggtga caggaacatt catcagggtg gaaatttcat tcacggtaac aaattctgtt
     2101 actttaagaa ttttcttttc ttcttcctcc tttaaatgct cttcttcgat ttgttgctgc
     2161 accatatctc ttttcaggcg gcggtatttt gatgcttttg acttggtagg gcccgtgagc
     2221 ttggcaagag tgtcgcggat ttgtttttga atttcttctt cagatacttc cgggtgcaga
     2281 ggctctttac tttttcggct gtcgtgcctt gacgttcgtg aggtgccagt gccgcttccc
     2341 gtgcccccgg ttttgccttg catttccgac tcgcccggtt gaattccttc ttttttgatg
     2401 cgtttacgcc tgcgctttct gtccgaaact ttattgtccg acgaggtggc tacaggttgt
     2461 ttttgtggcg gtttttcaac cggtaactca atttttccca atactttagg gccatgcagt
     2521 ttaatgtatt cggtggctat caggttattg tcttccttga cttcttcttt ggcaggagca
     2581 atttcttctg taaccacggg tggttcttct gcaactacct tttcagctat ttcttctttg
     2641 acttccggtt ctggttctac aggagtttct gtcttttttg ctttttttgt ggtttttttc
     2701 tcttccggtg cctctatttt ttcttcggca acttcagttt tggcttttga tttttttgtc
     2761 tctttggcag tttcttcagc aactttttta ccgggttttt tttcgctgac tttttccagg
     2821 tcgatttttc ctacaacttt taaccctttt aatttgcccg ttttttgggt ttcttcggtt
     2881 tcttcttcct ctttttcaga agctttttca gaagcttttt caacttcttt gattttttct
     2941 tctacttgtt ttggggcttt tttggctggt ttttcagtag atgatttttc tggttcaaca
     3001 gtctggggtt cctcttttat agcttttttg gctggttcgg cggctttttg ggcgggttct
     3061 tccttttttt caataatttt tgattctacg ggataagaaa ccggaacatt ttttataaaa
     3121 agctctttat cctgggtgag gtcggtatct ttttcgcggt ctttggcctt gtcgagggtg
     3181 atagtttcgt ggcgtacact gccaatatca agtttcttag atgtttcttt tacctgcttg
     3241 tcctttgaat attctttttg cagaatttca taaatatccg aggtgatttt accattgggg
     3301 ttgttatcaa cctttattcc tttttttgaa agaaaatcaa tgatagaatt cacggacaca
     3361 ttgaattcgg tagcaatttt acttaatcgg tatgttttta cggtttcttc cattttttac
     3421 ctgattgaac cttatttttg ttttgcgtga ttagtatgaa aaatatagtt tgggtatttt
     3481 attcaaattc tgatcttaaa attttgataa cttctctgat ggtttcttct tccaggtcgg
     3541 tgcgttttac cagttcgtcc acgctgagtt cgaggacact tttggcggta tcgcaaccaa
     3601 cattttttag ttcttcgatg atccattctt caatttcatc tgcaaattcc tgcagatcaa
     3661 catcttcaaa atctgaatcc gaatcacggt acacatcgat ttcatagccg gtaagttttg
     3721 ctgccagttt gatattatat ccgcctttgc cgatagccag cgacacctga tcgggtttta
     3781 aaaagacatc agctttttta tttttttcat caatttttat tgaggaaact ttagccgggc
     3841 tgagtgcgcg ggttatatac aaggatatat tggtggtaaa gttaataaca tcaatgtttt
     3901 cgtttcgaag ttcgcgcact atgccgtgta tgcgggcacc tttcatgccc acgcaggcgc
     3961 ccacggggtc gatgcggtcg tcgtaagatt ctacagctac tttggcgcgt tcgccgggga
     4021 tacgtacaat tttcttaata gttatcaggc catcaaatac ttcggggacc tcagcttcaa
     4081 aaagacgttc gagaaacacg ggggaggtgc gtgaaaggat aatgaccggt gtgccgtttt
     4141 tcatttccac tttggaaact acagcacgta ccgattcgcc tttacggtag aaatcagagg
     4201 gaatttgttc ggattttggt atgataagtt caataccgtc gtcatcaagc accaggatct
     4261 cttttttcca cacctgatag atttcgccgg ttacaatctg gcctactttt tcaatgtatt
     4321 ctttataaat attgtctttt tcatgctcca ttatttttga aaccaggttt tggcgcatgg
     4381 cgagtatttc gcggcggccg aagtccttta gcctgacttc ttctgaaacg tcttcgccga
     4441 tttcaaagtc gggctcaatc tttatggcat ctgaataggc aatttgctgg ttggggtcgg
     4501 taacttcatt gtcatcaaca attttccggt tacgccatac ttcaaggtca cccttatcga
     4561 tatttacgat gatgtcgaag ttttcgtcgc taccgtattt ttttatcagc atatgcctga
     4621 aaacgtcttc aagtatgcgc atcatggttt cgcgctcaat atttttgtat tctttaaatt
     4681 cagaaaatga ttcaacaaga ttgatgtgtt ccattattca gtactttaaa tattatttgt
     4741 tactaatttt tattatgatt tttgcttctt taatgtcttt taatgcaagg atgatttcct
     4801 gttccggagt ttttttcttt ttattttcag gaatcagcag ggtaatgtct tttg
//
LOCUS       DAOSFP010000165         4847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15598_length_4847_cov_4.058222, whole genome shotgun sequence.
ACCESSION   DAOSFP010000165 DAOSFP010000000
VERSION     DAOSFP010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15598_length_4847_cov_4.058222"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PLI16_05910"
     CDS             complement(<1..752)
                     /locus_tag="PLI16_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84131.1"
                     /translation="MKKFYLSLLLSIFMLTGFQASSQIIISAMMPNPVSSDSAFEYVQ
                     LMATQNIDFSTTPYSVVLLNNGTVGAKGWANGLNVSYKYNLTSGTVSTGDVFYVGGEG
                     KRIDGTGSTDISSLVWIRAINNVTTTGDDFGNPNATGIFGNGGANADGVGVFAGTTVD
                     SLSVPIDAMFYGSGIGTAYVAGPPEKGYKMPTNDHYSETAGLFGSGTNTYFVPATTLQ
                     DTLLSFTGTYDTTMNAWTINRTPTKIKLTSSS"
     gene            complement(818..>2289)
                     /gene="dnaK"
                     /locus_tag="PLI16_05915"
     CDS             complement(818..>2289)
                     /gene="dnaK"
                     /locus_tag="PLI16_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOH84132.1"
                     /translation="RQATKEAGEIAGLNVKRIINEPTAAALAYGLDKKTVDLKVAVYD
                     LGGGTFDISILELGDGVFEVKSTNGDTHLGGDDFDHNVIEWLAQEFKNEENIDLRKDP
                     MALQRLKEAAEKAKIELSSSTETDINLPYITSVDGVPKHLVKKLTRAKFEQLCDEQFR
                     ATIEPCRKALSDANLKASDIDEVILVGGSTRIPKIQKMVEDFFGKVPSKGVNPDEVVA
                     VGAAIQGGVLTGEVKDVLLLDVTPLSLGIETLGGVMTKLIESNTTIPIKKSETFSTAS
                     DSQTSVEIHILQGERPMASGNKSIGRFHLDGIPPAPRGIPQIEVTFDIDANGILNVSA
                     KDKATGKSQQIRIEASSGLNDQEIKRMKDEAAANAETDKAAKETADKINAADSMIFTT
                     EKQLKEYGDKIPAEKKAPIESALKELKDAHQSKNLQAIDAALEKINSAWQAASQEMYN
                     ATQQGQGAPNDPGQQQQQQNPGGGSDNVTDVDFEEVKDK"
     assembly_gap    2290..2299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2300..2737)
                     /locus_tag="PLI16_05920"
     CDS             complement(<2300..2737)
                     /locus_tag="PLI16_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOH84133.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGKRTTPSIVAFTT
                     NGERKIGDPAKRQAITNPQNTIYSIKRFMGETFDRMAKEINRVSFKVERGENNTPRVK
                     IADRLYTPQEISAMVLQKMKKTAEDYLGQEVTDAVITVPAYFSD"
     gene            complement(2904..4391)
                     /gene="dacB"
                     /locus_tag="PLI16_05925"
     CDS             complement(2904..4391)
                     /gene="dacB"
                     /locus_tag="PLI16_05925"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOH84134.1"
                     /translation="MSRLIYNCIITFLLTLPVFAQNDINGAGALNAEIEALKKDAGLK
                     SASWSIFVIETETGNTVCAHNADVVLEPASVMKIVSTGAALSLLGASCTFETKLEYSG
                     TVDTVGTLHGDLFITGGGDPTIGSGRFDKTVSTDTVFNEFYLALQQNNIRQIDGYLVG
                     DASVFEENPAAGSWLWEDIGNYYASGVFGLNIYENSYRLYFNAGTTIGSRAELTRMKP
                     VMDELTFINNVTTAGAGTGDNVVIYGQAYTPLRMLEGTVPLGKSDFDVDGAIPDPPRY
                     FVQQFASYLNNQGVKIEKSFTTDREMKWKNQSDTAERKLLAKHISPPVSDIVIQTNMK
                     SINLFAEAIVKACGVKMKKEGSENAGIEVVKNFWSSKNVNLTGFVMEDACGLSRKNKI
                     STRQLAEMLRVLTKEKSYKAFRRSLPVAGQSGGMASMLKNTVAEKNLIAKTGNMDQIK
                     SYAGYVTNASGKELAFALVFNNYSCNNAALKQKAEKLLLLISQTK"
     gene            4561..>4847
                     /locus_tag="PLI16_05930"
     CDS             4561..>4847
                     /locus_tag="PLI16_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004336196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84135.1"
                     /translation="MQKGKINVQTENIFPVIKKFLYSDHDIFLRELISNAVDATQKLK
                     MLSSVGKFKEELGDLTIQVIADEKKKTLTIKDRGIGMTAEELDKYINQIAFS"
BASE COUNT         1259 a         1091 c         1029 g         1458 t
ORIGIN      
        1 aacgaagaag atgtcagttt tattttagta ggtgttctgt taatggtcca tgcattcatt
       61 gttgtatcgt atgttccggt gaatgaaagc aaggtatcct gcaatgtggt ggcgggtaca
      121 aaataagtat tcgttccgga tccaaacaat ccagctgtct cactgtaatg atcatttgta
      181 ggcatcttgt atcccttttc gggtggcccg gcaacataag cagttcctat gccagatcca
      241 taaaacatag catcaatagg aacacttaaa gaatccaccg tggtacctgc aaacacgcca
      301 actccatcgg cattggcgcc gccgttgcca aatatgcccg tagcattagg attaccaaaa
      361 tcatcaccgg tggtggtaac attattaata gcacgtatcc aaaccaggct gctgatatct
      421 gttgatccgg taccatctat tctctttccc tcaccgccaa cataaaacac gtcaccggtg
      481 ctaacagtgc cactggtcaa attatattta taagaaacat tcaggccgtt ggcccagcct
      541 ttagccccaa ccgttccgtt atttaacaaa acaactgaat atggagtggt tgaaaaatca
      601 atattttgag ttgccataag ttgtacatac tcaaatgccg agtctgaact caccgggttg
      661 ggcatcatgg cagagattat aatttgagac gatgcctgaa aacctgtcag cataaaaatg
      721 cttaacaaca aggatagata aaactttttc ataagacttt ttttttgcaa atatattact
      781 attttttgaa ttaaagggtg atttctcacc ctttaactta tttatcctta acttcttcaa
      841 aatcaacgtc agtaacattg tcgctgccgc cacccgggtt ttgctgctgc tgttgctgtc
      901 ccgggtcatt tggtgcgccc tgaccctgct gcgtagcatt atacatttcc tggctggctg
      961 cctgccatgc ggagttaatc ttttccagtg cagcatcgat ggcctggagg tttttgctct
     1021 gatgcgcatc tttaagttct ttcaaggcgc tttcgatagg agctttcttt tctgccggta
     1081 ttttatcgcc gtattcttta agttgctttt cggtggtaaa aatcatcgag tcggctgcat
     1141 tgatcttatc agcagtttct ttggctgctt tgtcggtttc ggcgttggct gcggcttcgt
     1201 ctttcatgcg ttttatttcc tggtcgttca accctgatga agcttcgatg cgtatttgct
     1261 gcgactttcc ggtagcttta tctttggccg acacattaag gattccgttg gcatcaatat
     1321 cgaaagtaac ttcaatctgc gggattccgc ggggggcagg tggaatgccg tcgagatgga
     1381 aacgtcctat actcttgttg cctgaggcca taggtctttc accctggaga atatgaattt
     1441 caacagaggt ctggctatca gaggcagtag aaaaagtttc tgactttttt ataggtatgg
     1501 tagtgttcga ttcgatgagt ttggtcatca ccccgccaag ggtttcaata ccgagtgaca
     1561 atggagtgac atcgagaagt aacacgtctt tgacttcgcc agtgagcaca ccgccttgta
     1621 tagctgctcc cacggctacc acttcgtcgg ggtttacgcc tttggaaggt acttttccga
     1681 aaaaatcttc taccattttc tggattttgg ggatacgggt ggatccgccc accaggataa
     1741 cttcgtcgat gtcggaagct ttcaggttag catcggacag ggccttgcgg cagggttcta
     1801 tggttgccct gaactgttcg tcgcacaatt gttcaaattt ggcacgggta agtttcttca
     1861 cgagatgctt gggtacgcca tccaccgaag tgatgtaagg caaattgatg tcggtttcgg
     1921 tggaactgga aagttcgatt tttgcttttt cggctgcttc tttgagacgt tgcagtgcca
     1981 tagggtcttt acgcaggtcg atgttttctt catttttaaa ttcctgtgca agccattcga
     2041 tgacattatg gtcaaagtcg tcgccaccca ggtgggtatc gccattggtt gactttactt
     2101 caaaaacgcc atcgcctaat tcgagtatcg agatatcaaa agtacctccg ccaaggtcgt
     2161 acacggctac tttgaggtcc acggtttttt tgtcaaggcc gtaggccagt gcggctgcag
     2221 taggctcatt gatgatacgc tttacattaa gcccggcaat ttcaccggct tccttggtgg
     2281 cctgacgctn nnnnnnnnna tcgctgaaat aagcaggcac ggtgataacg gcatcggtta
     2341 cttcctgccc gaggtaatct tcggcagttt ttttcatttt ttgcaatacc atggcagaaa
     2401 tttcctgcgg agtgtataac ctgtccgcaa tttttactct tggggtatta ttttcgccac
     2461 gttccacttt aaaagaaacc cgattgattt cttttgccat gcggtcgaat gtttcgccca
     2521 tgaaccgctt tatcgagtaa atagtgtttt gaggattggt gatggcctga cgcttggcag
     2581 gatcgcctat cttacgttca ccatttgttg taaaagcaac gattgacgga gtggtgcgtt
     2641 ttccttcact gttgggaatt acaacaggtt cgttaccttc cattactgcc acacatgagt
     2701 tggtggtccc taagtctatt ccaataattt ttcccatttt attttttcct ttctgatttt
     2761 ttcaatttgg ttttgacagc aatagtcaag ctttatgcca atgcctctta atcatttttt
     2821 cgggaaaata tgtcataatt ttcttcagaa gaccaaaaac catgacacag aataatgaca
     2881 aaacgacaga aaaacaataa tcgctacttc gtttgcgata tcaaaagcaa aagtttttca
     2941 gctttttgtt tcaatgctgc attgttgcag gaataattat taaaaacaag tgcaaaggcc
     3001 agctcttttc ctgaggcatt agtaacataa cctgcatatg attttatctg atccatattg
     3061 ccggttttgg cgataaggtt tttttcggcc acagtatttt tgagcatgga ggccatgccg
     3121 cccgattgtc cggccacagg cagggaacgc cgaaaagcct tgtacgactt ttccttggtt
     3181 agcacgcgca gcatttcggc aagctgccgg gtgctgattt tgttttttct cgacagtccg
     3241 caggcatctt ccatcacaaa accggtaagg ttcacgtttt ttgacgacca aaaattttta
     3301 acaacctcaa tgccagcatt ttcagaacct tcctttttca tttttacacc acaagcctta
     3361 actatggctt cggcaaaaag gttaatgctt ttcatattgg tttgaatgac aatatcacta
     3421 accggtggcg atatgtgctt agcgagtagt ttccgctctg cggtgtcgct ttgatttttc
     3481 cacttcatct ccctgtccgt ggtaaatgat ttttctattt tgacaccttg attattcaag
     3541 tatgaggcaa actgttggac aaaatatcgc ggcgggtcgg gtatagcacc gtccacatca
     3601 aaatccgatt ttcccagcgg caccgtgcct tccaacatcc tgagcggagt gtatgcctgt
     3661 ccgtaaataa ccacattatc accggtaccc gccccggcag ttgttacatt attaatgaaa
     3721 gtgagctcat ccatcaccgg tttcattcta gtaagttctg ccctgctgcc gatagtggtt
     3781 ccggcattaa aatataagcg gtaagaattt tcataaatat taaggccaaa aaccccggac
     3841 gcgtaataat ttccaatgtc ttcccaaagc caactgcctg cggcaggatt ttcctcaaaa
     3901 accgaagcgt caccaacaag gtagccatcg atttgtcgta tattattttg ttgcaatgca
     3961 agataaaact cattaaaaac cgtatcggta ctgacagttt tgtcgaagcg gcctgagcca
     4021 atagtagggt cgccacctcc ggtaataaaa aggtcgccat gcagggttcc cacagtgtca
     4081 acagttcccg agtattccaa ctttgtttca aaagtgcagg aagcccccag caaagaaagt
     4141 gcggctccgg tgctgacaat tttcatgaca gaggccggct ccagcaccac atcggcatta
     4201 tgggcacata cagtatttcc tgtttctgtt tcgatgacaa aaatactcca ggaagcactt
     4261 tttagtccgg catctttttt caaagcctct atttcggcat tcagtgcgcc ggccccatta
     4321 atgtcatttt gagcaaaaac cggcagcgta agtaaaaaag taattataca attatatata
     4381 agcctgctca taacagattt ttcacaaaaa taataaaagc atggattaca acttatgtga
     4441 cttaaaacat aataagccag caaaatatct gcaaaacact atttttatgg tcattttgtc
     4501 agtgccgggc cgttggcata ccctttgaag aaaaccggcg aaccataata aattactact
     4561 atgcaaaaag gaaagataaa cgttcagaca gaaaacattt tccctgttat caaaaaattc
     4621 ttgtactccg atcacgatat atttctgagg gagcttatat ccaatgcggt tgatgctact
     4681 caaaaactaa agatgctttc ttcggttgga aaatttaaag aagaactcgg cgacctgacc
     4741 atacaggtca ttgcagatga aaagaaaaaa accctgacca taaaagaccg gggcataggc
     4801 atgacggccg aagagctgga caagtatatc aaccagatag ctttttc
//
LOCUS       DAOSFP010000166         4833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15667_length_4833_cov_3.405818, whole genome shotgun sequence.
ACCESSION   DAOSFP010000166 DAOSFP010000000
VERSION     DAOSFP010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15667_length_4833_cov_3.405818"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="PLI16_05935"
     CDS             <1..1341
                     /locus_tag="PLI16_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOH84136.1"
                     /translation="ALALSERSTGESAWLILGLTGEAFAIGLQSIWVALGCVTGIIFI
                     WTVMSNRLRLEAEKTQSLTVSGILYKRFPGSEKTIGLLSSSIVVFFFLFYVEAQFFGS
                     GKVLEQTFGIPAFWGVIIGSLIVVLYTMIGGFITVVYTDVFQAILMIISLVALPVMLI
                     FIIASNNVNVSNALIEAGNGYNSLTGGKTGVPAVLLILSGLSWMFGYTGQPQLLTRMM
                     AIKDKKHIHDAKVVAVIWTLIAYAGALVIGILGFVLMKNNFLGAHAASLTADAEKILP
                     VLINTFVGPVIAGLLLSGAISAMMSTASSEIIVSSSSITEDIYSNLAKKKFDGKKLLT
                     FNRLVTLGVGLLAFVLALTVKDSVYGLVSYAWSGIGSSFGPALLLLLFWKKMSRAGVI
                     ASLVSGTAGTIVWKLFFAVPTGVSERLSSFVFAFLMAVLFSYIFPEKNKTSAIE"
     gene            1334..2203
                     /locus_tag="PLI16_05940"
     CDS             1334..2203
                     /locus_tag="PLI16_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84137.1"
                     /translation="MNKNLLFFVSDLHGKESRYEKLFAAIAAERPGLVLIGGDILPLG
                     ILRNILKNAGPVDFVKSYLRPQLEALKKELSDDYPCIGVILGNDDPRSEEASIMELEK
                     SGLWHYIHFRQFQYDDFSICGYSYVPPTPFRLKDWERYDVSRYVDPGCYAPDEGFRTM
                     PVSDDEIQYATMMKDISSLTENLDMGKTIMLFHSPPYDSYLDRAALDGMQVDHVPLDV
                     HVGSIAVQRFIAEKQPFITLHGHIHESSRITGQWKQDFGNTKSFSAAYHGEELALLKI
                     DLNFPENVVRELL"
     gene            2285..>4833
                     /locus_tag="PLI16_05945"
     CDS             2285..>4833
                     /locus_tag="PLI16_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH84138.1"
                     /translation="MKLIKKIFDTNKYYLQDRSFMLLMQKRIYHVLLVCSAYDAFMLE
                     EDGRIDEQIFNEYTSLNLRYPPRFFIASTHDEAMNYLQTETIDLIITMLSVADPLELT
                     KKIKASFPAKPVVVLFPFSREVTEVYTEEDLSIYDHKFCWLGNADLLLAIIKLIEDKM
                     NVMHDIEKVGVQTILLVEDSTRFYSSYLPVLYKVIFQQSREFMAEGLNEHQKMIRMRG
                     RPKILLAKNYEEAEYFYATFKNNLLGIITDMSFLRQGETDPEAGLKICRMAKKDDETL
                     PVLIQSSDDKHYYTAQQMNAKFLNKNSKTLSAELRDFINEYMVFGDFIFRNPNTGEEI
                     SRASDLQSLQQKIFEIPDESLKYHFDRNHFSKWLNARALFTLGEIFKSVHVEDFSSLE
                     ETKHYLFDNIASYRMHKSRGIVAEFVSDSFDEYIFFARTGKGSLGGKARGLAFIDSLI
                     KRNRILDMFENVIITIPRTVVLTTDVFDEYMEQNKLYKTGLSEMTDEDILKEFVKGRL
                     PYRIHEDLLAFVHVVKNPIAIRSSSLLEDSYYQPFAGIYSTYMIPRVDNERKMIEMLS
                     CAIKSVFASVFFKASKSYMASTSNIIDQEKMAIILQEVCGKQYDNIFYPTFSGVARSI
                     NFYPIHPEKSEDGVANIAFGLGKYIVEGGTSIRFSPYYPKNILQLSSPQLALKETQNR
                     FMALDLNPESFVPSTDDGVNILKLPVSGVVKDGSLKHLVSMYDMNNNMLHDTYDPAGK
                     KIVSFSKILNYNTFPLADILKTLLETGQREMNNPVEIEFAVNLDVPEGYPKVFNFLQI
                     RPIVQNKEEIHEKLDSMDKSGLIISSNMAMGNGVYNTIRDIIYVKSETFDSS"
BASE COUNT         1291 a         1001 c         1104 g         1437 t
ORIGIN      
        1 gccttagccc tttccgaacg atccacgggc gagtcggcct ggctgatact tggccttacc
       61 ggcgaagctt ttgccatagg cttacaatcc atttgggttg ctttagggtg tgtaaccggg
      121 attattttta tctggacggt gatgagtaac cgtctgcgcc tggaggccga aaaaacacaa
      181 tcgctcaccg tgtcaggtat attatataaa cgttttcccg gtagtgaaaa aacaatagga
      241 ctgctttcat cgtccattgt agtttttttc tttttgtttt atgtggaagc ccagtttttt
      301 ggaagcggga aggttttgga gcaaactttt ggcatcccgg cattctgggg tgttattatc
      361 gggtcgttga ttgttgtttt atacaccatg ataggaggtt ttattaccgt ggtttacacc
      421 gacgtttttc aggctatcct gatgataata tccctggtgg ccctgccggt aatgttgatt
      481 tttatcatag cttccaataa tgtaaatgta agtaatgccc tgatagaagc aggcaatggc
      541 tataattccc tcaccggcgg caagaccggt gttccggccg tgcttttaat cctcagcggc
      601 cttagctgga tgtttggcta tactggtcag ccacaattgc tcacccgcat gatggctatc
      661 aaagataaaa agcacataca cgatgcaaaa gtggtagcag taatatggac gctgattgct
      721 tatgcaggag ccctggtaat aggaattttg ggatttgttc tgatgaaaaa caattttctg
      781 ggggcccatg cagccagctt gacagccgat gccgaaaaga tcttgccggt gcttattaat
      841 acttttgtgg ggccggtaat tgccggactg ttgctgtcgg gtgccatatc ggccatgatg
      901 tccactgcat cttctgaaat aatagtcagc tcctcgtcaa ttaccgagga tatttacagc
      961 aaccttgcca aaaagaaatt tgacggtaaa aaactgctca cattcaaccg cctggtaaca
     1021 ctgggcgtgg gcttgcttgc ctttgtgctt gccttaacag ttaaggattc tgtttacgga
     1081 ttggtatctt atgcatggtc aggaataggg tcgtctttcg ggccggcgct gctgctcctg
     1141 ttgttttgga aaaagatgtc ccgtgccggg gttattgctt cccttgtttc gggtacggcc
     1201 ggtaccatag tgtggaaatt gttttttgcc gttcctacgg gcgtttccga gcgtctgagc
     1261 agttttgttt ttgctttcct catggctgtg ttgttttctt acatttttcc tgaaaaaaat
     1321 aaaacttcgg caattgaata aaaacctttt attttttgtt tctgaccttc acggcaaaga
     1381 aagccgttat gaaaaacttt ttgcagccat cgccgcagaa aggccaggtc tggtgttgat
     1441 aggaggggat atactcccgt tgggaatcct tagaaacatt ttgaaaaatg ccggacctgt
     1501 tgattttgta aaatcatacc ttcgaccaca actggaagca ttaaagaaag aactttcaga
     1561 tgattatccc tgtattggtg tcatacttgg taatgatgac ccgcgttcgg aagaagcttc
     1621 gatcatggaa cttgagaaat cagggttatg gcattatatt catttcaggc aatttcagta
     1681 tgatgatttt agcatttgcg ggtattccta tgtccctcca acgcctttcc ggctcaaaga
     1741 ttgggagcgc tatgatgttt cgcgttatgt ggaccccggt tgttatgcgc ctgacgaggg
     1801 ttttagaacc atgcctgttt ccgatgatga aattcaatat gctaccatga tgaaagacat
     1861 ttcgtcgctg acggagaacc tcgatatggg caaaacaatt atgctctttc attcaccgcc
     1921 ttacgattca tatctcgaca gggcagccct cgacggtatg caggtggatc acgttccgct
     1981 ggatgtgcat gtgggaagca ttgccgtaca acgttttatc gcagagaaac aaccgtttat
     2041 cacgttgcat ggccacatac atgaatcttc aaggattacc ggacagtgga aacaggattt
     2101 tggcaatacc aaatcatttt cggctgccta tcacggagaa gaactcgccc tgctgaaaat
     2161 tgaccttaat ttccctgaaa atgtggtgag ggaattattg tagttttgaa aaaatgcagg
     2221 aatatttcct ttctttttca attaattcat aatattttgt tactttgtag tagattcatt
     2281 cgatatgaaa ctgattaaaa aaatattcga caccaataag tactatttgc aggaccggtc
     2341 ttttatgctg ttgatgcaga aacgtattta tcatgtttta ctggtgtgca gtgcttatga
     2401 tgcttttatg ctggaagaag acggccgcat tgacgagcag atttttaatg aatacacctc
     2461 cctgaatttg cgctacccgc cgcgcttttt tattgcttcc acgcacgatg aagctatgaa
     2521 ttaccttcag acagaaacta ttgatctgat tattaccatg ttgagtgtcg ccgatccact
     2581 ggaacttaca aaaaaaatca aagctagttt ccctgccaaa cccgtggtgg tgttgttccc
     2641 attttcgcgt gaggtaacgg aagtatatac cgaagaggat ctgtcgattt acgaccataa
     2701 gttttgctgg cttggtaatg ccgatctgtt gttagccatc ataaagctta ttgaagataa
     2761 gatgaatgtg atgcatgata ttgagaaggt gggcgttcag acgattttgc tggtggaaga
     2821 ttccacccgt ttttattcga gttacctgcc ggtgctgtac aaggtcatct ttcagcagtc
     2881 gcgcgagttt atggccgaag ggctcaacga acaccagaaa atgatcagga tgcgcggcag
     2941 gccaaagata ctgttggcta aaaactatga agaagctgag tatttctatg cgacctttaa
     3001 aaacaatctg ctgggcatta ttaccgatat gtcgtttttg cgccagggcg aaaccgatcc
     3061 cgaagccggc cttaaaatat gccgcatggc caaaaaagac gacgaaaccc tgccggtgct
     3121 catacaatct tctgatgata agcactatta tactgcgcaa cagatgaatg caaagttttt
     3181 aaataagaac tcaaaaacct tgtctgccga gctgcgcgac ttcatcaacg agtatatggt
     3241 ttttggtgat tttattttca ggaaccccaa tacgggggaa gagatttcgc gggcctccga
     3301 cctgcagtcg ctgcaacaaa aaatatttga aatccctgat gaatcattga aatatcattt
     3361 cgaccgcaac cacttttcga aatggctgaa tgccagggca ttgtttaccc tgggagagat
     3421 atttaaaagt gtgcatgtgg aagatttcag cagtcttgaa gaaactaaac attacctgtt
     3481 cgataatatc gccagctatc gtatgcacaa gtccagaggt attgttgctg aatttgtgag
     3541 cgacagtttt gatgaatata ttttctttgc ccgcaccggc aagggttcgc tgggaggtaa
     3601 agccagaggg cttgctttta ttgactcgct gattaagcgc aaccggattt tggatatgtt
     3661 cgaaaatgtc atcatcacca ttccccgtac cgtggtgctc accaccgatg tgtttgatga
     3721 gtatatggaa caaaacaaac tttacaaaac cggcttgtcg gaaatgacgg atgaggatat
     3781 tctgaaggaa tttgttaaag gccgcctgcc ataccggatt catgaagatt tgctggcctt
     3841 tgtgcatgtg gtaaaaaatc cgatagctat tcgttcgtcc agcctgctgg aagattccta
     3901 ttatcagcct tttgcaggga tatattctac ttatatgatc cccagggttg ataacgaacg
     3961 caaaatgatt gaaatgctca gctgtgccat caaatcggtg tttgcttcgg tgtttttcaa
     4021 agccagcaaa tcctacatgg catctacttc caatattatc gaccaggaaa aaatggccat
     4081 catactgcag gaagtatgcg gcaagcaata cgacaatatt ttctatccca ctttttcggg
     4141 cgtggcgcgt tcgataaatt tttatcctat ccatcccgaa aaaagcgaag atggcgttgc
     4201 caatattgcc tttggcctgg gcaaatatat tgtggaaggc ggcacttcca tccgcttttc
     4261 accttattat cccaagaata ttttgcagtt gtcgtctcct caacttgccc tgaaagaaac
     4321 acaaaacagg tttatggcgc tggaccttaa tcccgaaagt tttgtgcctt ccaccgacga
     4381 tggagtcaat atcctcaaac tgcctgtcag cggagtggtt aaagatggat cgttaaagca
     4441 tctggtatcg atgtatgaca tgaacaacaa tatgttgcac gatacgtatg atccggctgg
     4501 aaaaaaaatt gtctcttttt caaaaatttt aaattacaac acatttcctt tggcagatat
     4561 cttaaaaact cttttggaaa ccggccagcg cgaaatgaac aacccggtgg aaatagaatt
     4621 tgccgtaaat cttgatgtgc ctgagggata tcccaaggta tttaattttt tgcagatacg
     4681 gcctatagtt caaaacaagg aagagataca cgaaaagctg gatagtatgg ataaaagcgg
     4741 gttaatcatc agttccaata tggccatggg gaatggcgtt tacaatacca tacgcgatat
     4801 tatctatgtg aaatccgaaa cctttgattc ttc
//
LOCUS       DAOSFP010000167         4825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15699_length_4825_cov_3.207338, whole genome shotgun sequence.
ACCESSION   DAOSFP010000167 DAOSFP010000000
VERSION     DAOSFP010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15699_length_4825_cov_3.207338"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PLI16_05950"
     CDS             <1..751
                     /locus_tag="PLI16_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84139.1"
                     /translation="TNDVASFTTTILATDSSQFDVAWKLFTVPLTAYAGQDIYIAFHE
                     HVANNTIEGDVIGLDMVTINSPLSPVAQCSPLSWDAGNVTLASSETSGNFTLSNTGAG
                     TLTCSSINGLNAPFTTSLIPANVSLTAGGSLTFTFSYNPVIMGTDNQTVVIATNGGNV
                     SISLSGNGVTGIGEQDINVTAIYPNPVSDILHIAVRGEKSIEIYNLIGEMVASHRNMD
                     EVNISGLSVGTYIVKVITGSKIHTGKFSIIR"
     gene            861..2135
                     /locus_tag="PLI16_05955"
     CDS             861..2135
                     /locus_tag="PLI16_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOH84140.1"
                     /translation="MQTNAPLAEQMRPETIGDYIGQQHLVGDNAILRKAIESGNIPSM
                     ILWGPPGVGKTTLAYIISQQLQRPFFTLSAVHSGVKDVRSVIEKAGESSEKAILFIDE
                     IHRFNKAQQDSLLGAVEKGVITLIGATTENPSFEVISPLLSRCQVYILKNLEKKELLL
                     FLDKGSKMLTENTGKHIVIKETEALLRISGGDARKLLNALELVVNASINKEKEVIITD
                     DLVLKIVQQNIALYDKLGEQHYDIISAFIKSLRGSDPNAAVYWLARMVKGGEDPKFIA
                     RRMLILASEDIGNANPNALLLATNCFQAVQMIGYPECELILSQTAIYLACSSKSNASY
                     QALQNAHALIDQTGDLPVPLHIRNAPTKLMKEIGYHQGYKYAHDFENNFTELEYLPEK
                     ISGKKLYDPQQNPRENELRNFLKIRWKNKYKY"
     gene            2201..3712
                     /locus_tag="PLI16_05960"
     CDS             2201..3712
                     /locus_tag="PLI16_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84141.1"
                     /translation="MEPSWEVDVNAPLLKSSLGINNILPDSMIGINNDNSLSLVYKYS
                     FYDFSFDSLVDFPDTISQKDFPLPGGFPVNPGQTFFNFTENSNLSISGAQVTKVDFHS
                     GFLALEVFSSIPEPVIVTYTIPNTTNNGLPLQVTETVPAAPPGTTASFLKLFDISGYS
                     MDMSGPTGIDANIITSNIKAMMHPSAHTYYLDSLDKFAFNVRFKDISIDYAKGYFGSH
                     SFAFGPDTTSIDVFSKISSGSFDFEDIKLHLNVENGFGADLQVTFDDITAINSKTSQA
                     VSLNAGIIGQNINISRAVETGDPGQPVSPSVYNYNLDNSNILDMIENIPDMISYRIKV
                     ATNPMGNISSGNDFVYYNNPLKAILDMEIPLSLIASNLTLTDTVDFDLGENTGTIESG
                     TLSLYADNGFPFSAQVQLYLLNDNMQVTDSLLFPDMITAPALNGNYMVIRPARSVIQI
                     PLPGEKISRLYQTKKMRVVARFNTAGAGHHVKIYEQYRLDLKLTGDFSYMIQL"
     gene            3728..>4825
                     /locus_tag="PLI16_05965"
     CDS             3728..>4825
                     /locus_tag="PLI16_05965"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOH84142.1"
                     /translation="MNKTYKFYRLIAAWLCFVSISGYSQQYYCENIDNSKYYLSLNSI
                     LEFNADGPTNYFAASFYKGRFLDNTMKTEALNRLRPNNNMLAYLFNTDIYLNIPGKKN
                     NINYYAGFENHNLMELSFNKNFFHLFFFGNKTFTGQSLSLDDLSYTTLNFQQLKAGIS
                     KTWQKNDASYILTAGVGFNNGQNLMKYEVPYGALFTQQNAEYLDLDMTIGMKRSDTTR
                     AGFGAENGWGFSADVAYVFRDENNIITCQVEDLGFIRWKKNSQSYSKDTLVHFDGFEI
                     SNLFDIDGETIRGISADSLINEFAFSKTQAGFSDMIPMRIFISYKRYLFKRWLSLTCS
                     FNSYAFSVYRPQLCLTGSLNIPVKNSVVSISP"
BASE COUNT         1371 a         1118 c         1014 g         1322 t
ORIGIN      
        1 aaccaatgat gtcgcaagct ttacaacaac aatattagcc actgattcaa gccagtttga
       61 tgtcgcatgg aaattattta ctgtgccgct tactgcatat gccggccagg atatttacat
      121 tgcttttcat gaacatgtgg ccaataatac catcgaagga gatgtcattg gtttagatat
      181 ggtaaccatc aattcgccac tcagccctgt ggctcaatgt tctccgttat cctgggatgc
      241 cggtaatgtt acactggcat catccgaaac ctcgggcaac ttcaccctgt ccaataccgg
      301 ggccggcacc ctgacatgca gcagtattaa cggattgaac gcacctttta ccaccagcct
      361 gattccggca aatgtaagtc tgactgcagg cggatcgctg acatttactt tttcctacaa
      421 ccctgtcatt atgggtacgg ataatcaaac cgtggttatt gcaactaatg gcgggaatgt
      481 ttccatcagt ttatcaggaa acggcgtcac aggcatcggt gaacaggata ttaacgtgac
      541 ggccatttat cccaatcctg tcagcgatat attgcacatc gctgtcaggg gtgaaaaatc
      601 aattgaaatt tataacctga taggcgaaat ggttgcttcg cacagaaata tggatgaggt
      661 caatatttcc ggcctgtctg ttggaacata tattgttaag gtgattaccg gatcaaaaat
      721 acataccggg aaattcagca ttattcgctg attatggtga acaaaattat tagtgaggct
      781 gcccgtgccg ggcagctttt tttataatct tataaccccg gttaatatta cttttttgta
      841 tttttgtgat ttaaataaaa atgcagacaa acgctcctct tgcagaacaa atgcgcccgg
      901 aaactatagg ggattacatc ggccagcaac atctggttgg cgataatgcc atactccgta
      961 aggccatcga atcgggcaat atcccctcta tgatactttg gggcccaccg ggagtgggta
     1021 agacaacact tgcatatatc atttcgcaac agttgcaacg gccgtttttc acgttgagtg
     1081 cggtacattc gggagttaaa gatgtgagaa gtgttattga gaaagctggt gaaagctcgg
     1141 aaaaagccat attattcatc gatgagatcc atcgttttaa taaggcacag caggattcgc
     1201 tgctgggagc agtagaaaag ggtgtgatca cactgatagg cgccactacc gaaaatcctt
     1261 cttttgaagt tatatcgccc ctcttgtccc ggtgtcaggt atatattctg aaaaatctgg
     1321 aaaaaaaaga acttctgctc tttctcgaca aaggaagtaa gatgctgaca gagaacaccg
     1381 gaaagcatat tgtcataaag gaaactgagg cgctactgcg tatttcgggc ggtgatgccc
     1441 gcaaactgct gaatgcgctg gaacttgtcg taaacgcaag cataaacaag gaaaaagagg
     1501 tgattattac tgatgacctg gttttaaaga tcgttcagca gaatattgca ctttacgata
     1561 aacttggcga gcaacactac gacattattt cggcttttat caaatcgttg cgcggcagcg
     1621 atcccaatgc agctgtttac tggcttgccc gtatggttaa gggcggcgaa gaccccaaat
     1681 tcattgcaag gcgaatgctc attctggcat cggaagatat cggcaatgcc aaccccaacg
     1741 cgttgctgtt agcgaccaac tgctttcagg ccgtacaaat gattggttat cccgagtgtg
     1801 agctgatact gtcgcaaacg gctatttacc ttgcctgttc gtcaaaaagt aatgcttctt
     1861 atcaggcttt acaaaatgcc catgccctca ttgaccagac cggtgacttg cccgtaccac
     1921 ttcatatacg taacgcaccc actaaactga tgaaagaaat tgggtatcac cagggctata
     1981 aatatgctca tgattttgaa aacaatttta cagagctgga atacctgccc gaaaaaattt
     2041 cgggcaaaaa actttacgac ccgcaacaga atccgcgcga aaacgaactg cgtaattttc
     2101 taaaaatcag atggaagaac aagtataagt attaaccttc atgttgaaaa aatttctgat
     2161 acttttttta ttttgtctac tgctgttttc gtgccgcaaa atggaaccgt cgtgggaggt
     2221 ggacgtcaat gcgcccctgc ttaaatcatc gctgggaatt aacaatatcc tgcccgactc
     2281 gatgattggt atcaacaatg ataactccct gagtttggtg tataaatact ctttctatga
     2341 tttttctttc gattcgctgg ttgactttcc tgatacgatt tcccaaaaag actttccgtt
     2401 gcccggagga tttcctgtaa accccggcca gacatttttc aatttcactg aaaattccaa
     2461 cctttccatt tctggtgcac aggtgacaaa agttgatttt cattcaggtt ttctggcact
     2521 ggaggtgttc agctctatac ccgagcctgt tatcgtaact tatacaattc caaataccac
     2581 aaataacggg ttgcccttgc aggtaaccga aacagtgcct gcagccccgc ccggcaccac
     2641 ggcatccttt ctaaaactct ttgatatctc gggatacagc atggatatga gcggaccaac
     2701 agggattgac gccaacatca ttaccagcaa cattaaggct atgatgcatc cctcggctca
     2761 tacctattat ctcgacagtt tagataaatt tgcctttaat gtccggttta aagacatttc
     2821 catagactat gcaaaaggat atttcggtag ccatagtttt gcgtttgggc ctgacaccac
     2881 cagcatagat gttttcagca agatttcgtc cggcagcttt gattttgaag atattaagct
     2941 ccatctgaat gtagaaaacg gttttggcgc tgatctgcaa gttacttttg atgatatcac
     3001 agccattaat tccaaaacgt cgcaggctgt ttcgctcaat gccggcatta tcgggcaaaa
     3061 catcaacatc tcacgggcgg tcgaaaccgg cgatcccggc caacctgtaa gcccttctgt
     3121 ttataactat aacctggata actctaacat tttagacatg atcgagaata tccccgatat
     3181 gatttcatac aggattaaag tggctaccaa tccgatgggg aatatttctt cgggcaatga
     3241 ttttgtttat tataacaacc cgctgaaagc catactggat atggaaatac ccttgtcact
     3301 aattgccagc aacttaacct taaccgatac ggttgatttt gacctgggcg aaaatacggg
     3361 gaccatcgaa tcaggtacct taagcctgta tgccgataac ggatttcctt ttagcgcaca
     3421 ggtacaatta tatcttttga acgacaatat gcaggtaacc gactcgttgt tgttccccga
     3481 catgataacg gctccggcgc taaacggcaa ctacatggta atccggcctg cacgttccgt
     3541 catacaaatt cccttgcccg gcgaaaaaat atcccgcctg tatcagacaa aaaaaatgcg
     3601 tgttgtggca cggttcaaca ccgccggtgc cggccatcac gtgaagattt acgaacagta
     3661 caggctcgac ctgaaactta ccggcgattt ttcatacatg atacagttgt aaccaatgac
     3721 gtgcccgttg aataaaacat ataaattcta ccggttaatt gcagcgtggc tgtgttttgt
     3781 ttccatatca ggatactcac aacaatatta ctgtgaaaat attgacaaca gcaaatatta
     3841 tctttcgctc aattcaatat tggaatttaa tgccgacggg cccaccaact actttgccgc
     3901 atcattttac aagggccggt ttctggacaa caccatgaaa acggaggccc taaaccgcct
     3961 tcgccccaac aacaatatgc tggcctattt gttcaatacc gatatttacc ttaatatccc
     4021 ggggaagaaa aataatatca actattacgc agggtttgaa aaccacaacc tgatggaact
     4081 tagcttcaac aaaaactttt tccacctctt ctttttcggg aataaaacct ttaccggaca
     4141 atcattgtct ctggatgacc tatcctatac tacactgaat tttcagcagc ttaaagccgg
     4201 catcagcaaa acctggcaaa aaaacgatgc ctcttatatc ctgacggcgg gtgtgggctt
     4261 taacaatggc cagaacctca tgaaatatga agtgccttac ggcgccttat ttacccaaca
     4321 aaatgcggaa tacctcgatc tcgacatgac tatcggcatg aaaaggtcag acacgacccg
     4381 cgccggcttt ggcgccgaga acggttgggg attttcggcc gatgtggctt acgtattccg
     4441 tgacgaaaac aatatcatta cctgccaggt ggaagacctt ggctttatcc gctggaaaaa
     4501 aaattcgcaa agttattcca aagacaccct ggtgcatttt gatggttttg aaatcagcaa
     4561 cctgtttgac attgacggcg aaaccatccg agggataagt gcagacagcc tgataaatga
     4621 gttcgcattt tcgaaaacac aagccggctt ttctgacatg attcctatgc ggatttttat
     4681 cagctacaaa aggtatttgt tcaagcgctg gctctcattg acatgcagtt tcaacagtta
     4741 tgccttttcg gtttacaggc cgcagttgtg cctgacgggt tcgctgaata ttcccgtgaa
     4801 aaattcggtc gtcagcatct cccct
//
LOCUS       DAOSFP010000168         4821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15727_length_4821_cov_3.690516, whole genome shotgun sequence.
ACCESSION   DAOSFP010000168 DAOSFP010000000
VERSION     DAOSFP010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15727_length_4821_cov_3.690516"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1624
                     /locus_tag="PLI16_05970"
     CDS             398..1624
                     /locus_tag="PLI16_05970"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOH84143.1"
                     /translation="MNKVTEKHLVGQPIFKQLINLLPREKFDRLVLQYESDRYYKTFT
                     SWDQLVTMLFGILNRCDSMVEVCDGMRVLQGKLNYLGMDSSPAKSTAGDGLRNRPNDL
                     FMDFYFALLDHFTPLLSVSRIKDVSFDKFFIFDSTTIRLFSEIMKGVGRNPKNDGKKK
                     GGLKVHMMIDAHSNTAKFVKISEAKLHDKNFLKYLNLPPHSMIVFDKAYNHYLQFAQW
                     TQQQVNFVCRLKNNAVYEVQEVLFEQTLNDKEFGVMKEEHIHLKYKEKKVWKTLCLRK
                     VTYRDEKGRVYQFITNNFEISNKEVAFLYKKRWDIELLFKKLKQNFQLHYFYSETENG
                     IKTQVWITLIAHLILTVLKAITKTAKAFSTVAALVRMHLISHLDIYWVIESCRRTYTK
                     KGQSRNKSPTPQLCLF"
     gene            1783..4197
                     /locus_tag="PLI16_05975"
     CDS             1783..4197
                     /locus_tag="PLI16_05975"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84144.1"
                     /translation="MIKKYFLLAVALLCVAASGFSQEATVKGVIYNGTTKETIPGVSV
                     MTSDKTGVSSEIDGSFTVKVHSGKAVRLVFKILGYANVTKLLDIKPGETKEIKVEMME
                     QATAIEGVVVTAGKFEQKFSDVTISMDVLKPNQIENRNTNNITSSLNKVSGVDINDNQ
                     PSIRGGSGYSYGAGSRVMVLVDDLPMLSPDGGDSKWNYLPLENLSQVEIIKGASSALF
                     GSSALNGVINLRTAYPKSKPETKISVNSGLYLNPKNRSAAWWYDSDPLYSHTVAGKMV
                     NPLSMFGVKNPGFGGLNFFHSRQIGQFDLVVGGNLYENQGFQEDEGEARARANINFRY
                     RAKKIEGLSLGFNANYMYQDKKNFFLWKDSDSGIYRQNNPAVPVTGYRINVDPYFLYF
                     NKRGSKYTLKTRYYRQTNHNADSTMNNDADIIYADYQYQHKFKDKYNLTAGAMGSYSK
                     STALLYQTDHHATNAAIYIQFDAKVWKRVSFSLGLRGEYARIDKDETESSFSIRTKKD
                     TFNFPIEPVLRAGINVQAAEYTFVRASFGQGYRFPSIAEKFIRTSIGGLNIFPNPDLN
                     AESGWSAEIGLKQGFRVGSWLGYVDVAGFWTQYKNMMEFTFNDYRKDGRTDTTYTGYE
                     DYIKKFLGFKSLNVGHARIAGVDFSLMGKGLLFGFPATLGLSYIYTDPVNLDYDATDT
                     NSFKVLKYRNYHTIKGDFEVQVAFFTLGLSYIYKSHIINIDKAFEGELIPGISKSNLL
                     PGLKQYRTEHDKGYHVLDLRMLFDINKQHRIGLFVNNLFNTEYMTRPGYIEAPINVAV
                     QYMVTF"
     gene            4636..>4821
                     /locus_tag="PLI16_05980"
     CDS             4636..>4821
                     /locus_tag="PLI16_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84145.1"
                     /translation="MKKILLFHFVFLSCSFLALAQQATIKGTIFNGKSKETIPGVNVI
                     LDEKTGVASDIDGRYSLK"
BASE COUNT         1516 a          901 c          993 g         1411 t
ORIGIN      
        1 cgctctgata tgcaatttac tcatggctgt aatacaaaaa acactcaaac gctcatgggg
       61 cttttcaaat ctggtaagct tttgtaaaat tcatctgttt aactatcttc acctattaaa
      121 ttttcttgaa aatcctgata aagactggag aaaagtgcaa tgtaaattgg aacagcttgc
      181 actattttag ggggggctta cttttgcaat ttaataaaac ataagattta atagttgatt
      241 ctcggcatgt tatgatttac aaaagaaaat tttatatttt taccggacaa caatgattta
      301 ttgtcactac tagccgacta aattttttaa aagaagggta cttccgttag ggttcccctt
      361 catgttgtaa ttttgttttg cataacacaa aaacaacatg aacaaagtta cggaaaaaca
      421 tttagtcggt cagccgattt ttaaacaatt aataaattta cttcccagag aaaagtttga
      481 ccgattagta ttgcagtatg aatcagatcg ctattataaa actttcacat catgggatca
      541 actcgtcacg atgctatttg gtatactgaa tcgctgtgat tctatggttg aagtttgcga
      601 cggaatgcga gtattgcagg gaaagttaaa ttatttgggg atggatagtt cgccagccaa
      661 aagcactgct ggtgatggat taaggaacag gccgaatgat ctttttatgg atttctattt
      721 tgcgcttctt gaccatttta ccccactttt gtcggtcagc cgaatcaaag atgtttcgtt
      781 tgacaaattt ttcatatttg actcaaccac aataagacta ttttccgaga tcatgaaagg
      841 tgttggccga aatccgaaaa acgatggcaa aaagaaaggt ggcttgaaag tacacatgat
      901 gatcgatgcc cattccaata cagcgaaatt tgtgaagatt agtgaggcga agttgcatga
      961 caaaaacttt ctcaaatacc tgaacctacc gccccatagc atgattgttt ttgacaaagc
     1021 ttataatcat tacttgcagt ttgcacagtg gacgcagcag caagtgaatt ttgtttgtag
     1081 acttaaaaac aatgctgttt atgaggtgca agaggtgctg tttgagcaaa cgctgaacga
     1141 taaagaattt ggtgtgatga aagaggagca cattcacctg aaatacaaag aaaaaaaagt
     1201 ttggaaaaca ctttgtttgc ggaaagtaac ctatagagac gaaaaaggac gagtatatca
     1261 atttattaca aacaactttg aaattagcaa caaagaagtg gcattcttgt ataaaaaacg
     1321 ctgggacatt gagctgttat tcaaaaaact taaacagaat ttccagttgc actattttta
     1381 ttctgaaaca gaaaatggca ttaaaacaca agtctggatt accttgattg cacatttaat
     1441 tctcacggta cttaaagcaa tcacgaaaac cgccaaagct ttttctaccg ttgcagcatt
     1501 ggttcgcatg catttaatta gccatcttga tatttattgg gttattgaaa gttgcagaag
     1561 gacatataca aaaaaaggcc aatcaagaaa caaaagccct acaccacaac tttgtctatt
     1621 ctgaaaaaaa cgagggggta tgttttttaa ttccgaaaag tcgaaacctc aatttgttga
     1681 attactgcta ttttcaaata aaaattcaga aatatgcaga tttgtcggtt gatagtgatt
     1741 tattgtataa atttgcaccc gaaaaaatcc aaaatgtttt ttatgataaa aaaatacttt
     1801 ctactggccg ttgctttact ttgtgtggcg gcttccggtt tttcgcagga agctaccgtg
     1861 aaaggtgtta tttacaatgg tacaaccaaa gaaactatcc ccggggtgag tgtaatgacg
     1921 agtgataaaa caggggtaag ttccgaaata gatggttcat ttaccgttaa agtgcattcg
     1981 ggtaaagccg tcaggctggt atttaaaatt ctcggctatg ccaatgtaac caaactgctg
     2041 gatatcaaac cgggcgaaac aaaggaaatt aaggttgaaa tgatggaaca ggctactgct
     2101 attgaaggag tggtggttac cgctggtaag tttgaacaga aattttcgga tgttactatt
     2161 tcgatggatg tgttgaaacc caatcagata gaaaacagaa ataccaacaa tatcaccagt
     2221 tcgctgaata aagtttctgg cgttgatatc aatgataacc agcccagtat tcgtggcggc
     2281 agcggatata gttatggtgc gggcagccgt gttatggtgc tggtggatga tttgccgatg
     2341 ctttcaccgg atggcggcga ttcaaaatgg aattacctgc ctcttgaaaa cttatcacag
     2401 gttgaaatta ttaaaggcgc ctcttcggcc ttgttcgggt cttcggcatt aaacggagtt
     2461 attaaccttc ggacagctta tccaaaatca aaacctgaaa ccaagatttc tgtaaacagc
     2521 ggtttgtatc tgaaccctaa aaaccgtagc gcggcctggt ggtacgacag tgatccatta
     2581 tattcacata ctgtggctgg gaaaatggta aaccccctct ccatgtttgg ggtaaaaaac
     2641 ccgggatttg gcggtttaaa cttctttcat tcccggcaaa taggccaatt tgaccttgtt
     2701 gtaggcggca acctatacga aaaccagggt ttccaggagg atgaaggtga agccagagcc
     2761 agagccaata tcaatttcag atatcgggca aaaaaaattg aaggattgtc cctgggtttc
     2821 aatgcaaatt atatgtatca ggacaagaaa aatttctttt tatggaaaga ctcggattca
     2881 ggaatttacc ggcagaacaa tcctgctgtt cccgttaccg ggtaccgtat caatgttgac
     2941 ccgtattttt tgtatttcaa caaacgtggc agcaagtaca cccttaaaac caggtattac
     3001 aggcaaacaa atcataatgc tgacagcacc atgaataacg atgccgacat aatctatgcc
     3061 gattaccagt atcagcataa attcaaggat aagtataatc tcacggctgg cgcaatggga
     3121 agctacagca aatccaccgc actgttgtac caaaccgatc atcatgctac caacgctgcc
     3181 atttacatac agtttgatgc taaggtttgg aaacgagttt cgttttcgct tggtttgaga
     3241 ggcgagtatg cccgcattga taaagatgag acagaatctt ccttttcgat aagaacaaaa
     3301 aaagatacct ttaactttcc gatagagcct gtgctgcggg ccggaattaa cgtgcaggca
     3361 gcagaatata cctttgtcag ggcctccttc gggcagggat accgcttccc atcaatcgcc
     3421 gaaaagttta tcaggacatc aatcggggga ctgaatattt ttcccaatcc ggatttaaat
     3481 gccgagtcgg ggtggagcgc cgaaatagga cttaaacagg gtttcagggt tggctcctgg
     3541 ctgggatatg tggatgtggc cggtttctgg acacaatata aaaatatgat ggaatttact
     3601 tttaacgatt accgcaaaga tggccggact gatactactt atacaggata tgaagattat
     3661 attaagaaat ttcttgggtt taaatcttta aatgttggcc atgccagaat cgcaggagtg
     3721 gatttctcgc tgatgggcaa aggcctgctg tttggattcc cggcaaccct tggcctgagc
     3781 tatatttaca ccgatcccgt taaccttgat tatgacgcca cggataccaa cagttttaag
     3841 gttttaaaat acagaaatta tcatacgata aaaggtgatt tcgaagtaca ggtggctttt
     3901 ttcacactgg gactttccta tatctataaa agccacatta tcaatatcga caaagctttt
     3961 gaaggcgaac tcatccccgg catttccaag tcgaacctgc tcccgggcct taaacaatac
     4021 cgtacggaac acgacaaagg ataccatgtc ctcgacctta ggatgctttt tgacattaat
     4081 aagcaacata ggataggact atttgtaaat aacctgttca acaccgaata catgacacgg
     4141 cccggatata tcgaagcccc tatcaatgtg gccgttcaat atatggtaac tttctgatgg
     4201 gtgtcgaatt tttgtgcctg atttttcacc cggagggccg gcacaagaaa ttctcgtaat
     4261 cagaaagagc tgtcggttca gatattttcc tggcagggat tatcctgata tgatacattc
     4321 tctacttacg ctcattcaga aatttcacat catttatttt aaaacagaag gggttaatcc
     4381 caatcaaaaa ttatttgttg atttgacaat taagattttt taacaaaaaa aagctacgtt
     4441 ataccataaa tttaaattgt ttgtttttgc gccttatctg ctgataatca gcgtattgat
     4501 ttttgtgcat gaatgatttt ttaacgacag tcataatata gtgttgataa tcagctcatt
     4561 aaacaggatt ggtattatta attattttgt atttttgcgc tccgaaaata tgcgggttaa
     4621 aataaaagca ttctgatgaa aaaaattcta ctattccatt ttgtattttt aagttgttct
     4681 ttcttggcct tagctcaaca ggcaaccatc aaaggcacca tcttcaacgg aaaatcaaag
     4741 gagactattc ctggagtaaa tgttatcctg gacgaaaaaa ccggtgtggc ctccgacatc
     4801 gatggccggt actcgctaaa g
//
LOCUS       DAOSFP010000169         4792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15858_length_4792_cov_4.097952, whole genome shotgun sequence.
ACCESSION   DAOSFP010000169 DAOSFP010000000
VERSION     DAOSFP010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15858_length_4792_cov_4.097952"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLI16_05985"
     CDS             complement(<1..1004)
                     /locus_tag="PLI16_05985"
                     /inference="COORDINATES: protein motif:HMM:NF017739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HOH84146.1"
                     /translation="MPIEPSAHIIPSGEVVSFTILVENQEINSAYQIMSIAIIKEFNR
                     IAHAEIKLLDGSSAAQDFEISNSDDFIVGKNVEIKVGYHGENTSLFKGVIIRHGIKIL
                     ENEGSCLNITLKNKAYKSTLVRNNNVFSNVADSDIIREIISRYGLDNEVEATGVRHES
                     VMQFNCTDWDFINMRAEANAQLVYTADDRIIVKKPDLSADTMLTLQYGATMLEFEADL
                     DGRTSYSNYKASSWNFQEQELRDVQQACDTNELAQGNLTAADVARTLGQEQFNIHINT
                     SLPDENEITKLTDSFVQKNNLSRINGRVKSFGIPGVQPGDIIELRGVGSRFNGRAFVT
                     G"
     gene            complement(1007..1699)
                     /locus_tag="PLI16_05990"
     CDS             complement(1007..1699)
                     /locus_tag="PLI16_05990"
                     /inference="COORDINATES: protein motif:HMM:NF039279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84147.1"
                     /translation="MSILNDIFPGKLTKLTFKAFTDNTFTQEDSDAGEYTVMFNPSSL
                     AMSLSVQTEQQQAPGTTSSDIKFVSIKPQNYNIEFVIDGTGAAGGPPVDVAADVMKFL
                     NVVYHYKSEEHNSPNVMIKYGAVLLKCVLLDVNITYNLFSPSGKPLRAKVKCTFVSTL
                     NPDLSQMINSPCSPDITHKRVVKQNEQLVFLANKIYKKNDYYAEVAAKNKLNNFRKLK
                     PGTEIYFPPLSK"
     gene            complement(1696..1848)
                     /locus_tag="PLI16_05995"
     CDS             complement(1696..1848)
                     /locus_tag="PLI16_05995"
                     /inference="COORDINATES: protein motif:HMM:NF039278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HOH84148.1"
                     /translation="MPVQINELVIRAEIVNEQKSVQNNTPAKSKVKIQKMVDELVKIN
                     KNKNER"
     gene            complement(1867..2325)
                     /locus_tag="PLI16_06000"
     CDS             complement(1867..2325)
                     /locus_tag="PLI16_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOH84149.1"
                     /translation="MAFFEKYPLVGFHFKVTFLELGAAEIDSRFHEVSGLNAELTVEE
                     LSEGGENRFTYKLPVKAKFPNLVLKRALSPLPSVLVKWADDAIYNLEFWPCTVLVSLL
                     NEMHLPVKNWVFYSAYPVKIQASDLKAQDNSVMVETLELAYKYSKQINII"
     gene            complement(2339..2770)
                     /locus_tag="PLI16_06005"
     CDS             complement(2339..2770)
                     /locus_tag="PLI16_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOH84150.1"
                     /translation="MATKYPLPVFHFRVEWGGSSINFSEVSGLNVETQVIEYRDGASP
                     DFSSVKMPGLQKYGNITLKRGAMQGDNEFFDWWNTHQLNTIERRDVIISLLNEKHEPV
                     ITWRVRNAFPVKVDGGSLKATGNEVSIETLEIAHEGITVER"
     gene            complement(2846..4717)
                     /locus_tag="PLI16_06010"
     CDS             complement(2846..4717)
                     /locus_tag="PLI16_06010"
                     /inference="COORDINATES: protein motif:HMM:NF037565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HOH84151.1"
                     /translation="MATSYKTPGVYVEEIAKFPPSVAEVETAIPAFIGFTAQAKKILD
                     GDLKEKPKRITSLLEYEQYFGQGPDFIPGNIELDGNNMPVADNCTLTNSKFLLYDSIR
                     LFYDNGGGACYIVSVGTFGETEATLFADNTRFITGLDALKKYDEPTLIVFPEAVGLST
                     DSLGALQQAALKQCAELGDRFTIMDVQAQDSLDEALESFRNSVGMNNLKYGAAYYPYL
                     STIYSKSFRFRDINSKLTVKNRLTPTDVDEKGTTIISRIESLEKVITDNDNLTKEFTD
                     TLTALLNESDFKELSDDDKSKRLWNLTLQLDGKLATNGDADTGVITNSELLTAAAGTV
                     KINLLDTKIIDQLIYLNETNFNTVKDDLLSTSFKSMAVPSADPGTGKGLTDIFKAVKI
                     CFSLIVQMGTGYEETYEKAIIPYIPFYNNVLSVLNTKATLLPPGGAIAGIYSAVDRDR
                     GVWKAPANISVNSVNGVSEFINDAIQENMNVDPNAGKSINAIRPFTGKGIMVWGSRTL
                     AGNDNEWRYVPVRRFFNFVEESAKKSTGWAVFEPNDANTWTRIRGQLDNFLNNLWKRG
                     ALAGAKPEHAYYVNVGLGLTMTAQDVLEGRMIVEIGMAAVRPAEFIILRFSHKLQES"
BASE COUNT         1176 a         1100 c         1064 g         1452 t
ORIGIN      
        1 cccgtaacga aggcccggcc gttaaagcgt gagcccacac cacgaagctc aatgatatcg
       61 ccgggctgaa cgccaggaat accgaatgat ttcaccctgc cgttgattct cgacaaatta
      121 tttttttgaa caaacgaatc cgtcagttta gtgatttcat tttcgtcggg caaagaggtg
      181 ttgatatgga tattgaattg ttcctgaccc agcgtccggg caacatccgc agcagtgagg
      241 ttaccctgtg ccagttcgtt tgtatcgcag gcctgctgaa catctctcaa ctcctgctcc
      301 tgaaaattcc acgacgaagc tttatagttg ctataggatg tgcggccatc caggtccgcc
      361 tcaaattcca gcatggtggc cccgtattgc agggttagca tggtatcggc tgaaaggtca
      421 ggctttttta caatgattct gtcatcggca gtgtacacca attgtgcatt ggcttcggca
      481 cgcatattaa taaagtccca gtcggtgcaa ttgaactgca tcaccgattc gtggcgcaca
      541 ccggtagctt ccacttcatt gtccaaccca taacggctga tgatttcccg gatgatgtcg
      601 ctgtcggcga cattactgaa cacattattg tttcgtacca gtgtactttt gtaagcttta
      661 ttttttaagg ttatgttcag gcaagaacct tcgttttcaa gaatttttat gccgtgtcgg
      721 ataataaccc ccttgaaaag gctggtattt tcaccatggt atcccacctt gatttcgaca
      781 ttttttccga caataaaatc atcgctgttg ctgatttcaa aatcctgtgc agcggaactg
      841 ccatcgagca attttatttc ggcatgagca atcctgttaa actcttttat tatggcaatg
      901 ctcatgatct gataagcaga gtttatttcc tgattttcaa caagaatagt aaaggaaact
      961 acttcgccgg agggaataat atgggcagag ggttcaatag gcatagttat ttgcttaatg
     1021 gaggaaaata aatttctgtt ccgggtttca gttttcggaa attatttaat ttgtttttgg
     1081 cagccacctc ggcataataa tcgttttttt tgtatatttt gtttgccaga aaaactaact
     1141 gttcgttttg ttttaccact cttttatgag tgatatccgg tgaacaggga ctgttgatca
     1201 tctggctcag gtcgggattg agcgtactga caaaggtgca ttttactttt gcccgcaggg
     1261 gcttgccgct gggcgaaaaa agattgtagg taatattaac atcgagcagc acacatttta
     1321 gtaaaaccgc accatatttt atcatcacgt ttggcgaatt atgttcttcg gacttataat
     1381 ggtaaaccac attcaggaat ttcattacat ccgcggccac atccactggt ggcccgccgg
     1441 cagccccggt tccgtcgata acaaattcaa tattgtaatt ctgaggtttg atgcttacaa
     1501 attttatgtc gcttgatgtg gtgcctggcg cctgttgttg ttcggtctgc acgctaagac
     1561 tcatagccag cgaactcgga ttaaacatta cggtatattc tccggcgtcg ctatcttcct
     1621 gagtaaaggt attgtcggta aaggccttaa aagtaagttt ggtcagcttc ccgggaaata
     1681 tgtcattaag aatgctcatc gttcattttt gtttttattg attttaacca actcatccac
     1741 cattttttga attttcactt tgctttttgc cggagtgttg ttttgcacgg acttctgttc
     1801 gttgactatt tcggcacgta tcaccaattc attgatctgg acaggcataa gattttctgt
     1861 ttatggtcag ataatattta tttgttttga gtatttataa gccagttcca gggtttccac
     1921 catcacgctg ttgtcctggg ctttgaggtc cgaagcctga atttttaccg gataggccga
     1981 ataaaaaacc cagtttttaa ccggcaggtg catttcgttg agcagcgaaa ccaacaccgt
     2041 gcatggccag aattcaagat tataaatagc gtcatcagcc catttgacca gcaccgacgg
     2101 cagcggcgac agggctcttt tcagcaccag gttcggaaac tttgccttca cagggagttt
     2161 gtaagtaaac ctgttttcgc cgccttccga caattcttcc accgtgagct ccgcatttaa
     2221 tcccgacacc tcatgaaacc gcgagtcgat ttcagcagcg cccagttcaa gaaaagttac
     2281 cttgaagtga aaccccacta aggggtactt ttcaaaaaat gccatgactg cctgcttatt
     2341 aacgttctac cgtgatacct tcgtgagcaa tttccagggt ttcgatagac acttcgttgc
     2401 cggtggcttt cagggaaccg ccatccacct ttaccggaaa agcattgcgc acccgccagg
     2461 taatcaccgg ttcatgtttt tcgttcaaca gcgagatgat cacatcccga cgttcgatgg
     2521 tgttcaactg atgtgtattc caccagtcga aaaactcgtt atcgccttgc atggcgcctc
     2581 gtttaagggt tatgttgcca tatttttgca ggccgggcat tttgaccgat gaaaaatcgg
     2641 ggctcgcccc gtcgcggtat tcaatgacct gtgtctcgac attcaacccg gaaacttccg
     2701 aaaagttgat gctgctgccg ccccactcta ctctgaaatg aaatactggt aatggatatt
     2761 ttgttgccat aataaatcgt ttttttgttt gttattaatt ttctgtcagc ctccccaaaa
     2821 aacggggagg cattgttttt ttaaattaag attcctgaag tttgtgtgag aaccgcagaa
     2881 tgataaattc ggccggtcgc acggcagcca ttcctatttc aacaatcatg cgtccttcga
     2941 ggacatcctg cgcggtcata gtgaggccca gcccgacatt tacataatag gcatgttccg
     3001 gttttgctcc ggccagggcg ccacgtttcc agaggttgtt cagaaaattg tcgagttgcc
     3061 ccctgatcct ggtccaggtg ttggcatcat tgggttcaaa gaccgcccat ccggtggatt
     3121 ttttcgcact ttcttccaca aaattaaaaa agcggcgaac aggcacatag cgccattcgt
     3181 tatcgttgcc ggccagggtg cgcgagcccc acaccataat accttttccg gtaaacggcc
     3241 ttatggcatt aatggatttc ccggcattag gatccacatt catgttttcc tgtatggcat
     3301 cattgataaa ctccgagacc ccgttaactg aattgacact aatattggcc ggggctttcc
     3361 aaacaccgcg gtcgcggtca acagcggaat agatgccggc aatggcgccg cccggcggca
     3421 acagggtagc tttggtgttc agcacactca gaacattatt gtaaaacggg atgtagggta
     3481 tgatggcttt ttcgtaggtt tcttcataac ctgttcccat ctgcactatt aacgaaaaac
     3541 agattttcac agccttgaat atatccgtca accctttgcc ggtgccggga tcggcagaag
     3601 gaaccgccat tgatttgaac gaggtgctta gcaggtcatc tttcaccgta ttgaaattgg
     3661 tttcgttgag ataaatcaac tgatcaatga tttttgtatc cagcaggtta attttcactg
     3721 tacctgcggc agcagtcagc aattcgctgt tagtaatcac accggtatca gcatcgccgt
     3781 ttgtggccaa tttcccgtcc aactgaagtg tcaggttcca caatctttta cttttatcat
     3841 cgtccgaaag ttctttgaaa tcactttcgt tcagcagggc ggtgagtgta tcagtaaatt
     3901 ctttcgtgag gttgtcgtta tcggtaataa ccttttccaa agattctatc ctgctgatga
     3961 tggtggtacc tttttcgtcc acatcggtag gtgtcaaccg gtttttaacc gtcaattttg
     4021 aattgatgtc gcggaaccgg aaacttttgg aataaatcgt agagagatac ggataataag
     4081 cggcgccgta tttaaggtta ttcattccta ctgaattgcg gaacgactcc agggcttcgt
     4141 ctagtgaatc ctgtgcctgt acatccatga tggtaaatct gtcgccgagt tccgcacatt
     4201 gtttaagggc agcctgttgc agagcaccca gagaatcggt ggaaagcccg acagcttcag
     4261 gaaatactat cagggtaggc tcgtcatatt ttttgagggc atcgaggcct gtaataaatc
     4321 tggtattatc ggcaaacagc gttgcttcgg tttcgccaaa agtgccaaca gacacgatat
     4381 aacaggcgcc gccgccatta tcgtaaaaca ggcggatgct gtcgtacagc aaaaacttac
     4441 tgttggtcag tgtgcaatta tctgccaccg gcatattatt gccgtccaat tcaatatttc
     4501 ccggaataaa atcgggacct tgtccgaaat actgctcgta ttccagcagg ctggtaatcc
     4561 gcttaggctt ttctttcagg tcgccgtcaa gaattttttt ggcctgagcc gtaaacccta
     4621 taaatgcggg aatagcagtc tccacttcgg ccactgatgg agggaatttt gcgatttctt
     4681 ccacataaac tcccggtgtt ttgtaagatg ttgccataat gttttgtttg ttttttaatt
     4741 attatacttg ttatttttga tatatcgttt ttgaaaattt caaataatct gt
//
LOCUS       DAOSFP010000170         4788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_15878_length_4788_cov_4.361082, whole genome shotgun sequence.
ACCESSION   DAOSFP010000170 DAOSFP010000000
VERSION     DAOSFP010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15878_length_4788_cov_4.361082"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="PLI16_06015"
     CDS             complement(<1..1206)
                     /locus_tag="PLI16_06015"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOH84152.1"
                     /translation="MEKQATCPTIISKLNAYVVVLLAFSIPFPRQFSLYLSVLWIVLS
                     VTERIIEKRFSVKKPDWIILIGFFLFYLLHITGLFYSTNTSAAGVDIILRIPFVFMPL
                     LLALSAENLTRYKKKILLAFVAGNIVAALICLGASVFRSLSYVDGQWQWQAELMEHYG
                     YTFWQMLANGGNNFMYEPLSIFIHPGYFAIYMVTAVVILLDSLADKEAKNTVGTRSWI
                     LILIVFCSIMVYLLFSRTGLIALFLVLFFYFMLFVFRQKSKLLKGALLLGVALFTIAG
                     VYVFIHNGRMKNTYSQIRKYVNDPSDVSKNDDRIFIWGLSMNIIQENLVFGVGTGDSK
                     DELMEKYQEKQMEEAMKARLNVHNQYLETTIQLGMLGLLSLLFIFTSAFCRAAKRRDI
                     KLALVLFVNA"
     gene            complement(1213..2382)
                     /locus_tag="PLI16_06020"
     CDS             complement(1213..2382)
                     /locus_tag="PLI16_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOH84153.1"
                     /translation="MKHLSYITETFTESVIREMTRISDAMDGLNLSQGFPDFESPLEI
                     KEAAIKAINENWNQYPVTFGEPELREAISKKILNFNKIQADPATEITVTCGATEAMIA
                     TLKAVINPGDEIIIFEPFYENYGPDGILSGATPRYVKLHTPGWYIDFDELAAAFNEKT
                     KAIIINTPNNPTGKVFSKAEMEKIAALCIKWDTLAITDEIYEHILYDDSVHISLASLP
                     GMAERTITINSISKTYSVTGWRVGWAVAAPAITARIRKVHDFLTVGAPTPFQHAAAHA
                     LTFKPGYYHELREKYEKSRAYITETLVSTGFKPYIPQGAYYIMVDASGLFPLLKAEND
                     FDFSKKLIEKTRVATVPGNSFYAAKNFISGQVRFAFCKRFETLELMRGLFIENLK"
     gene            complement(2450..3556)
                     /locus_tag="PLI16_06025"
     CDS             complement(2450..3556)
                     /locus_tag="PLI16_06025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOH84154.1"
                     /translation="MSYKVRFVEPAKLYRMIKEELDNAYFEVMSKGDLIDRGQLKSFE
                     ENLARFVGTKYAVGLNSGYDSLHISLRAAGIGAGHEVIVPAHTFVASCSAIVNVGATP
                     VLVDVAKDFNIAVDKIEEAITSKTRGIMPVHLSGWMADMPAIMEIAKKYNLVVVEDAC
                     QSLGSSINGIGAGAWGLTGCFSFYPFKILGGYGDGGAITTNSEEVATFARRMRFNGED
                     RETGEYHGHGFTCLLDNLQAAFLDVKLKYMREWIKKRQAIANRYREALSDIPDLLLPH
                     YNVPGFDHVYQNYTLRSKQGSEFSDYLKENGVEVLTQFRKPYYKHEALKLKDTGFPET
                     EALSREVCSLPMNVEIDDDEVEYVIKVVRSFYGK"
     gene            complement(3575..4504)
                     /locus_tag="PLI16_06030"
     CDS             complement(3575..4504)
                     /locus_tag="PLI16_06030"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOH84155.1"
                     /translation="MRTAFFQALEDHAQPQTVFITGDLGFGVINGFLKKHPEKFINAG
                     VAEQNLIGLSAGMALAGKKVFAYSIANFNTLRPLEQIRNDVAYHKLNVTVVSVGGGLA
                     YGSLGISHHATEDLAILRSIPNMTVFAPGDTKEAYELTKRIICEDLGPVYLRLGRAGE
                     ATLHTDESVKKLVIGKAFPLIETSNRKLAILSTGGMLETAVKVCEQLKQQNIQSSVFS
                     YHTIKPFDIETTRRIFKEYELVVSLEEHSIIGGFSAAILESLVGSKGITLDNYLPFAL
                     PTTFTSKVGDQNYLRNLYNISPEKILEKILNTL"
     gene            complement(4504..>4788)
                     /locus_tag="PLI16_06035"
     CDS             complement(4504..>4788)
                     /locus_tag="PLI16_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH84156.1"
                     /translation="QSLAPVKDTINLEPFADKWQSFGWAVKMVDGHNCDELKDVLQGV
                     PFDKNKPNCIIANTIKGKGVSFMENQVLWHYRTPQGEEYDKAINELEGKI"
BASE COUNT         1352 a         1085 c         1000 g         1351 t
ORIGIN      
        1 cgcattgaca aacaacacca acgccagttt tatatcacgc ctttttgctg cccggcaaaa
       61 agcgctggta aatataaaca acaggcttaa aagcccaagc atacccaatt gaatggttgt
      121 ttccaggtat tgattatgaa cattcaggcg tgctttcatg gcctcctcca tctgtttttc
      181 ctggtatttt tccatgagct catccttgct atcgcctgta cctacaccaa aaaccaggtt
      241 ttcctgtatg atattcattg aaagtcccca gataaaaatc cggtcatcat ttttagaaac
      301 atccgacggg tcgtttacat actttcttat ctgtgaatag gtatttttca tcctgccatt
      361 atgaatgaaa acataaacac cggcaatcgt aaacagggct acacccagca acagtgcacc
      421 tttcagcagt ttactctttt gcctgaaaac aaataacata aaataaaaaa acaataccaa
      481 aaacaacgca ataagccctg tccgcgaaaa cagcaggtac accataatgc tacagaaaac
      541 aataagtatt aatatccatg acctggtgcc aaccgtattt ttagcttctt tatctgccag
      601 gctgtccaga agaatgacta cggcagttac catgtaaatg gcaaaataac ccggatgtat
      661 aaaaatggat aaaggctcat acataaaatt gttgccgccg ttggccagca tttgccaaaa
      721 ggtgtagccg taatgctcca tgagttcggc ctgccattgc cattgtccgt cgacatacga
      781 caatgaccgg aaaactgacg ccccgaggca tataagcgcc gcaacaatgt ttccggcaac
      841 aaaagccagc aggatttttt tcttgtatct ggtcaggttt tctgccgata aagccaacag
      901 caaaggcata aatacaaagg gaattctcag aatgatatcc accccggcag cactggtatt
      961 tgtactataa aacaggccgg tgatatgcaa caggtaaaac aagaaaaaac caataaggat
     1021 aatccaatcc ggctttttaa cagaaaatcg cttttcgata atcctttctg taaccgacag
     1081 cacgatccac aacacactca ggtacagcga aaactggcgc gggaaaggta tcgaaaaggc
     1141 taacagaaca acaacatacg cgttgagttt actgatgatt gtagggcatg ttgcttgttt
     1201 ttccaaaaca gcttatttca ggttttcaat aaaaagcccg cgcatgagct ccagtgtttc
     1261 aaatcttttg caaaatgcaa accgcacctg tcctgagatg aagttctttg cagcataaaa
     1321 agaatttccc ggaacggttg ccacacgggt tttttcgata agttttttcg agaaatcaaa
     1381 gtcgttttcg gcttttaaaa gtggaaaaag cccgcttgca tccaccatga tatagtaggc
     1441 gccctgagga atatatggct tgaaaccggt gctgaccagt gtttcggtaa tataggcccg
     1501 cgacttttcg tatttttctc gtagttcgtg gtagtagccg ggtttgaagg tgagtgcgtg
     1561 ggcggctgca tgctgaaatg gcgtgggcgc tccaacggtc aaaaaatcgt gtactttgcg
     1621 gatacgcgct gtaatagcag gagcagccac tgcccagccg acacgccagc cggtaacaga
     1681 ataggttttg gaaatggaat taatggtgat agtgcgttcg gccattcccg gcaacgaagc
     1741 cagagaaata tgcaccgaat catcataaag gatgtgttca taaatctcat cggtaatagc
     1801 cagggtatcc cattttatac ataatgcagc aatcttttcc atctcggctt ttgaaaaaac
     1861 tttccctgta ggattgttgg gagtattaat gatgattgcc ttggtttttt cattaaaagc
     1921 tgctgccaat tcgtcaaaat caatatacca gccgggtgta tgcagtttaa catacctggg
     1981 tgtagcgccc gataaaatgc catcgggacc ataattttcg taaaaaggtt caaaaatgat
     2041 gatttcatcg cccggattga tcacggcctt cagcgtggca atcatggctt cggtagcgcc
     2101 acaggttacg gtaatttcgg tggcagggtc ggcttgtatt ttgttaaaat ttaaaatctt
     2161 tttgctgata gcttcgcgca gctcaggttc accgaaagtt accggatatt gattccagtt
     2221 ttcatttatg gcttttatgg ctgcttcttt aatttccaat ggactctcaa aatcagggaa
     2281 cccctgggac aggttaagtc cgtccatggc gtcgctgatc ctggtcatct cacgtattac
     2341 tgattctgta aaggtttccg ttatgtatga taagtgtttc atcagagatt tattaatagt
     2401 actgtaagaa tgataacatg ccaaaactag ccattacaaa gacttccccc tactttccgt
     2461 aaaagctcct caccacctta atcacgtatt ccacctcgtc gtcgtcaatt tcaacattca
     2521 taggcaatga acatacttca cggctcagag cctcggtttc ggggaaaccg gtgtctttca
     2581 gtttcagagc ttcatgctta taatagggtt tgcggaattg ggtaaggact tctaccccgt
     2641 tttctttgag ataatccgaa aattcgcttc cctgcttaga acgtaaagta taattctgat
     2701 aaacatgatc gaaacccggc acgttatagt gtggcagcaa caggtcggga atatccgaca
     2761 gcgcttctct gtaacggttt gcaatggcct gccgtttctt tatccattca cgcatatact
     2821 ttagtttcac gtcgaggaat gccgcctgca aattgtcgag caggcacgta aagccatgcc
     2881 cgtggtattc gccggtttcc ctgtcttcgc cgttgaaacg catgcggcgt gcaaatgtag
     2941 ccacttcttc gctgttggtt gtgatagcgc cgccatcgcc ataaccgcct aaaattttaa
     3001 aaggataaaa actgaaacaa ccggtcagtc cccaggcgcc ggcgccaatg ccatttattg
     3061 atgagcccag gctttggcag gcatcttcaa ccaccaccag gttatatttc tttgcgattt
     3121 ccattatcgc aggcatatcg gccatccaac cgctgaggtg cacgggcata atgccgcggg
     3181 ttttggaagt tattgcctct tcaattttgt cgacggctat attaaaatct tttgccacat
     3241 caaccaaaac gggtgtagcg ccgacattga caatagccga acagctggca acaaaggtgt
     3301 gtgcaggtac aatgacttcg tggccggcac ctataccggc agcacgcagc gagatatgca
     3361 gtgaatcata gccgctgttc agtcctacgg catattttgt tcctacaaac ctggcgaggt
     3421 tttcttcaaa tgattttaat tgtccacggt cgataagatc gccttttgac ataacctcga
     3481 aataggcatt gtcgagttct tctttaatca tccggtacaa cttagccggc tcaacaaaac
     3541 ggactttata actcatacta tgtttttgct taatttataa ggtatttaaa attttttcca
     3601 ggattttttc tggagaaatg ttgtataaat ttctcaaata gttttgatcg cccactttag
     3661 aagtaaatgt tgtgggcaag gcaaaaggaa gatagttgtc aagagtgata cccttactac
     3721 cgaccaatga ttcgagaata gcagcgctga acccaccaat aatgctgtgt tcttcaagag
     3781 aaaccactaa ttcatattct ttaaaaatac gcctcgttgt ttcaatatca aagggtttga
     3841 tggtatgata actaaaaaca gaactttgaa tattttgttg ttttagttgt tcacaaactt
     3901 tgacagctgt ttcaagcata ccaccggttg ataagatagc cagcttgcgg ttggacgttt
     3961 ctatcagcgg gaaagccttg ccgatgacca gcttttttac actttcatca gtatgtaagg
     4021 tggcctctcc tgccctgccc agtctgagat atacagggcc aagatcttca caaatgatac
     4081 gtttggtgag ctcataggct tccttggtat caccgggagc aaatacagtc atattcggaa
     4141 tactgcgcaa aatggccaaa tcttcagtgg catgatgtga aatccctaag gatccgtagg
     4201 caaggccgcc acccacggaa acaacggtaa cattgagttt gtgatatgcc acatcgtttc
     4261 tgatttgctc caacgggcga agggtattaa aatttgcgat agaataagca aaaacctttt
     4321 tgcctgcaag tgccatgcct gcagacagtc caatcaaatt ttgttcagcg acaccagcat
     4381 ttataaattt ctcaggatgt tttttaagga agccattgat aacgccaaag ccaagatcgc
     4441 ctgtaataaa cacagtctga ggctgtgcat ggtcttctaa tgcctgaaaa aaagcagttc
     4501 tcattaaatt ttgccctcca actcattaat tgccttgtcg tattcctcac cctgtggtgt
     4561 ccggtaatgc cacaaaacct ggttttccat aaaagagact cctttgcctt tgattgtgtt
     4621 ggcaatgata cagttaggtt tattcttatc aaaaggcaca ccttgcagaa cgtctttaag
     4681 ttcatcacaa ttatggccgt caaccatttt aacggcccat ccgaatgatt gccatttgtc
     4741 ggcaaaaggt tcaaggttta tagtatcctt taccggggca aggctttg
//
LOCUS       DAOSFP010000171         4726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16176_length_4726_cov_4.163991, whole genome shotgun sequence.
ACCESSION   DAOSFP010000171 DAOSFP010000000
VERSION     DAOSFP010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16176_length_4726_cov_4.163991"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..498
                     /locus_tag="PLI16_06040"
     CDS             253..498
                     /locus_tag="PLI16_06040"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOH84157.1"
                     /translation="MRSIAHPMRINIIELLEANKEMNVTQIYEKLNIEQAAASLHLNL
                     LRTHGILNRRRSGKMTFYSLKNDSLNKLIDFVENCNK"
     gene            complement(506..919)
                     /locus_tag="PLI16_06045"
     CDS             complement(506..919)
                     /locus_tag="PLI16_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOH84158.1"
                     /translation="MIYQTEIILPSFGRGFHLITGIIKQNLPPLPETGLVNIFIRHTS
                     AGLTINENFDPTVRLDMSTAFDRIVPENTTLYQHNDEGSDDMPAHVKSSLAGASLSIP
                     IRNHSLGLGTWQGIFLCEFRSKKQQRTVLITIIGE"
     gene            968..2194
                     /locus_tag="PLI16_06050"
     CDS             968..2194
                     /locus_tag="PLI16_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84159.1"
                     /translation="MIFIRPIFFVVIYLLQTWIICGQTANSMYESGMSKYQQNDLAGA
                     ISEFTRCIEKNELFYDAYIGRGDCYTAQNKFGEALKDFSKALEIYPNYYPAYLKRAAT
                     YARAGENKKALADFARAIEIDKNKEGAYLERAKLYQKNNDLKSALTDLSTALEINGKN
                     ADVFFQRALIYELQKKDKAALSDFSKSIALNPLLAEAYYKRGLLLEKTGDYKAASADY
                     KKAVTLKIRHKELFLHKAQAQFFSGLWTEAIEDYNTLMNEYAVKDAGTYYNRAQCYYK
                     TDKTTEALKDFMKSAQLNTRNDSAYLYIARIYESQNNQKSAHIYYNKAISVNPENYNA
                     FAGRAKIFSGQQKFEQAVQDWNTALKSKPVAEWYFERALCKEAMKDKKGMCDDMKSAA
                     DLGYKKALQIYNGSCK"
     gene            2295..2927
                     /locus_tag="PLI16_06055"
     CDS             2295..2927
                     /locus_tag="PLI16_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84160.1"
                     /translation="MKNAKRFLILIVCGILLIPVFNSCKVGDEDAGFTFYTRKYRLCQ
                     DWSFSYYKHVVQHNDTIISQEYDGSSYIHIFGTQKFISNATMTISFKKNGTYNWEEHI
                     SNDTSVYTYKETGYWYFTGGGKDSDTKFKELLALQKTSHTKTIQIEGQSPVTDNYSAS
                     GNLETRVFRLIKLSIDEVKMESKVEQQYIEGTNTVLDGEITIINLAMGAQ"
     gene            2959..3552
                     /locus_tag="PLI16_06060"
     CDS             2959..3552
                     /locus_tag="PLI16_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84161.1"
                     /translation="MKKNTALPIFIFITVIISTMFFSCRKIDTMKLVGDWKVVSLSSS
                     YTFNNEKYETIFDGTTKTSKHLVSDSIVEENSYTGAIYTDYHSDGTYVYSETFKHNDT
                     GVTETLGVDGHWYFTGPNSEADWAVTDLLAMQATKSTFNTDMGNSHTTIYQGQNTLEL
                     YEITTLSKTKVVLKMQKAETINFTQYVTTMEFTLEPR"
     gene            3613..4446
                     /locus_tag="PLI16_06065"
     CDS             3613..4446
                     /locus_tag="PLI16_06065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH84162.1"
                     /translation="MRRIAKQTFTDTFHAIQQEHETYLRTDKTATFNSKVLIIAVSVC
                     LSLTCVEYIGKNDGYYLLLKFLGFIGLSNTAERLRFFIEHQTNQQLFSLCYWVVTILI
                     FYFLIPAAIIKFVFKENLKDYGLWGGSIFKERRIFGLFLIVMFPLILIFSTTESFQLR
                     YPFYHLRNDEALWSGFFIWELLYFFQFIALEFFFRGFMVHGLKKQFGYYSIFIMTIPY
                     CMIHFGKPFPETIAAIIAGIILGTLSLKSRSIWMGVAIHFIVALSMDFSALWQKGYWI
                     H"
     gene            4514..>4726
                     /locus_tag="PLI16_06070"
     CDS             4514..>4726
                     /locus_tag="PLI16_06070"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOH84163.1"
                     /translation="MEYKLDTIEEAIEDIRQGKIIIVVDDEDRENEGDFITAAETITA
                     EKVNFMAKHGRGLICAPISAGLCEALD"
BASE COUNT         1557 a          942 c          914 g         1313 t
ORIGIN      
        1 ataattttct ttacaaaaag gtgttctttt acctaaatat atgtagatta atacaataag
       61 gatttctttg taaagatccg gaatgttttt atgatgcatt aatcgtggac taagcgaata
      121 acttttttaa gtagataata atattaaaaa aaataagttt aattaataaa ttagaaatca
      181 ctattaacaa ttaaaaaaat gacactaagt aaaaaaatcg acgttaacaa atttgattca
      241 gccgcaagaa aaatgagatc aattgcgcat ccaatgagaa ttaacatcat tgaacttctg
      301 gaagctaaca aagaaatgaa tgttacccag atttatgaaa aactgaatat cgaacaggct
      361 gcagcatcac tgcatctgaa cctgctccgt actcatggaa ttctcaacag gcgcagaagc
      421 ggaaagatga cattttattc attaaaaaat gattctttga acaagctcat cgactttgtt
      481 gaaaattgca ataaataagg cgctattact cccctattat tgtaatcagg acagttcgtt
      541 gttgcttttt gcttctgaat tcgcataaaa atataccctg ccaggtaccc aatcccaggc
      601 tgtggttgcg aatcgggatg ctcaaagaag cccctgcaag gcttgattta acgtgagccg
      661 gcatatcatc actaccttca tcattatgct gatatagtgt tgtgttttcg ggaactatac
      721 ggtcaaaagc ggttgacata tccaatcgca ccgtaggatc aaagttctcg ttaatggtta
      781 gtcctgcaga ggtgtgcctg ataaaaatat tcactaaacc tgtttccggc aacggaggca
      841 ggttttgttt tataatgccg gtaatcaaat gaaatcccct tccgaaagaa ggtaaaataa
      901 tttctgtctg gtaaatcata ggattttttt gttgaaattt gctgtatcat ctcaaaggta
      961 taaaaaaatg atctttatcc gaccgatatt ttttgtcgta atttatctgc tgcaaacatg
     1021 gattatttgt ggacaaacag ccaattccat gtatgaaagc ggtatgagca aataccaaca
     1081 aaacgatctg gcaggagcca ttagcgagtt tacccgctgc atcgaaaaaa atgaactgtt
     1141 ttatgatgcc tacatcggca ggggcgattg ctatacggca cagaataaat tcggcgaagc
     1201 gctgaaagac ttcagtaaag cgctggaaat ttatcccaac tattacccgg cctaccttaa
     1261 acgtgccgcc acctatgccc gggcagggga aaacaaaaaa gcccttgctg attttgcccg
     1321 ggccattgaa attgacaaaa acaaagaagg cgcctatctg gagagggcaa aactatatca
     1381 gaaaaacaac gatttaaaat cggcccttac cgacctgagt acagccctgg aaataaacgg
     1441 taaaaatgcg gatgtttttt ttcaacgagc cttgatttac gagctccaga aaaaagacaa
     1501 agcggctttg agcgattttt cgaaaagcat tgcgttaaac ccactcctgg ctgaagcata
     1561 ttacaaaaga gggctactat tagaaaaaac aggagattat aaagccgcct ccgctgatta
     1621 taaaaaggcc gtcacattga aaatacgcca caaagaatta tttcttcaca aggctcaggc
     1681 gcagtttttt tcaggcttgt ggacggaggc tatcgaagac tacaacacac tgatgaacga
     1741 atatgccgta aaagatgccg gaacctatta taacagggcg caatgctatt acaaaaccga
     1801 taaaactacc gaagccctga aagattttat gaaaagcgcc cagctcaaca ccagaaatga
     1861 ttcggcatac ctttatatag ccagaattta cgagagccag aacaaccaaa aaagcgccca
     1921 tatatattat aacaaagcca tctccgtgaa tccggaaaac tacaatgcct ttgccggaag
     1981 ggccaaaatc ttttcgggac aacaaaaatt tgaacaggcc gtacaggatt ggaatacggc
     2041 tttaaaatca aagccggttg ccgaatggta tttcgaaaga gccttatgca aagaagccat
     2101 gaaagataaa aaaggcatgt gcgatgatat gaaatcggct gctgacctcg gctacaaaaa
     2161 agccctgcag atatacaacg gatcctgcaa atagtatgaa aacctttttt gttatcatgc
     2221 gtaaaaaaga taattttact tttttataga aattacttta aatttgttgt agttttatct
     2281 caaaaattca tgaaatgaaa aatgcaaaaa gattccttat tttaattgtc tgtggcatat
     2341 tgctaatccc tgttttcaat tcatgcaaag tgggtgatga agatgccggt tttacttttt
     2401 ataccaggaa atacaggtta tgtcaggatt ggagtttttc ctattataaa catgtcgtac
     2461 aacataacga caccattatc tcgcaagaat atgacgggtc atcttatatt cacatttttg
     2521 gcacccaaaa atttatttct aacgcgacga tgaccatttc tttcaagaaa aacggcacct
     2581 acaattggga agaacatatt tcaaacgaca cctcggtata tacgtataaa gaaacggggt
     2641 attggtattt tacaggaggc ggcaaagaca gcgacacgaa atttaaagaa ctacttgcct
     2701 tacaaaaaac ttcgcatacc aaaactattc aaatagaggg acaatcgccc gttactgata
     2761 attattctgc ttcgggcaat ctggaaacac gggttttcag gctcatcaaa ctttccattg
     2821 atgaagtgaa gatggagtcg aaagttgaac agcaatacat cgaagggacc aacactgttc
     2881 tggatggtga aatcaccatt ataaacctgg caatgggggc ccaataatta tcagtttcaa
     2941 aaaagaaaaa ttaataccat gaaaaaaaat actgctttac cgatttttat tttcatcaca
     3001 gtaatcatat ccacgatgtt tttttcgtgc cgcaaaattg acactatgaa gctggtgggc
     3061 gactggaaag ttgtttctct ttcgagcagc tatactttta acaatgaaaa gtatgaaacc
     3121 attttcgacg gcaccaccaa aaccagtaaa catctggtca gcgactccat cgtcgaagaa
     3181 aattcataca caggagccat atacaccgat taccacagcg acggcaccta tgtttattct
     3241 gaaacattta aacacaatga taccggtgtt actgaaactt taggtgtcga tgggcattgg
     3301 tacttcaccg gacccaattc tgaagccgac tgggctgtta ccgacctgct ggccatgcag
     3361 gcaaccaaaa gcactttcaa tacggacatg ggtaattcgc acaccacaat ttatcaggga
     3421 cagaacactc ttgaattata tgaaatcact accctgagca aaacgaaagt ggttttaaaa
     3481 atgcaaaaag ccgaaaccat taattttaca cagtacgtta ccaccatgga gtttaccctc
     3541 gaacccagat aaagcgtata aaaagtataa cataatcctt cgtatcacac ggaggatttt
     3601 ttttgtccag tcatgagacg catcgccaaa caaacattta cagatacttt ccacgccata
     3661 caacaggaac atgaaaccta cctcagaaca gacaaaacag ccacttttaa cagcaaggtg
     3721 ctgattattg ccgtaagtgt ctgcttatcc cttacctgcg ttgaatacat cggcaaaaac
     3781 gacggatact acctgctgct caaattttta ggatttattg gcttgagcaa cacggctgaa
     3841 cgactcaggt tttttattga acaccaaacc aatcagcagt tgttttcgct gtgctattgg
     3901 gttgttacaa tacttatctt ttattttctg attccggcag ccatcataaa atttgttttt
     3961 aaagaaaacc tgaaagacta cggattgtgg ggaggcagca tattcaagga acgcaggata
     4021 ttcggattgt ttctgattgt tatgtttccg ctgatactga ttttttcaac gaccgaaagc
     4081 tttcagttgc ggtatccttt ttaccatctg cggaacgacg aggcactctg gtctggtttt
     4141 tttatctggg aacttttgta cttttttcag tttattgcct tagaattttt cttccggggt
     4201 tttatggttc acggactgaa aaaacaattt ggatattata gcatcttcat catgaccatc
     4261 ccctattgta tgatacattt tggcaagcct ttccccgaaa caatagcagc catcattgcc
     4321 ggaataattt tagggacact cagtctgaaa agccgctcga tctggatggg ggtagccata
     4381 cattttattg tagccttatc aatggacttc tccgcattat ggcagaaagg atattggata
     4441 cattagtttg atgtaagttt cttttcgcgg attgcttgaa tgtttgtacc tttgcattgg
     4501 taaaacactc caaatggaat acaaattaga tacaatagaa gaagccatag aagatatccg
     4561 gcagggaaaa atcattattg ttgttgatga cgaagaccgc gaaaatgaag gcgattttat
     4621 cacagcagcc gagaccatca cagcggaaaa agtaaatttt atggcaaaac acgggcgcgg
     4681 gttgatctgt gcacctattt ctgctggctt atgcgaggcc ttagac
//
LOCUS       DAOSFP010000172         4707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16268_length_4707_cov_3.754729, whole genome shotgun sequence.
ACCESSION   DAOSFP010000172 DAOSFP010000000
VERSION     DAOSFP010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16268_length_4707_cov_3.754729"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PLI16_06075"
     CDS             complement(<1..1083)
                     /locus_tag="PLI16_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HOH84164.1"
                     /translation="MKRTLCFLAVAFMLAVLSVSESYGQAPQKFNYQAVCRDLSGNII
                     ANQAVTLRMTIHDLSATGAVLYQETHAVTTNNFGLVNVAVGAGTVVSGTFATIAWGTG
                     AKYLQIEVNTGSGYSSVGAPQLLSVPYALYANQANVPGVAGPTGPMGPTGPAGIPGPI
                     GPTGPYGPAGPTGLVGPVGPTGPVGPTGATGVMVTGTNMQTLRHNGTNWVANSILTNN
                     GTNIGINSVPQVGKKLYVYRPSGNFGPDSTGIYVYRYGSSSVATDGGSSWARTGVDAA
                     IKGYSFYGNNFTAGVAGYNYLDWSNSAGVIGGNALGTLFGALAFKDDLNTTWAGYFNG
                     NAKVTGTVTIAGGSPDVNKVLTATNTA"
     gene            complement(1102..1611)
                     /locus_tag="PLI16_06080"
     CDS             complement(1102..1611)
                     /locus_tag="PLI16_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84165.1"
                     /translation="MKKLLLFTLLTMLAASLIAQSTERYVLSGAGGTYLDNINNFQMD
                     YTIGEPVIITASSASIVLTQGFQQPFFLMTSLPETAEELPAWSLYPNPVTEHLFIVND
                     KPEQGTYIIQLFDMMGQMLVENMQIAESGTPLTLMMDFDGYATGNYFVRLLFQNKALE
                     TFNVIKINQ"
     gene            complement(1914..2450)
                     /locus_tag="PLI16_06085"
     CDS             complement(1914..2450)
                     /locus_tag="PLI16_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84166.1"
                     /translation="MKTKARKLVITVVAFFFCISGFGERAHAQFFTGGDINIGILGGL
                     NLNVAPIAGYRYKNFSVGVSPIIQYQATSTTGMAGQFSYGGAVFVEYSVWKGIFGHAE
                     FHITNSGYLQNSLGDIYKKGVWVMGCPIGVGYEHKLSGNLWIKTMLLYDPFLDIDIGA
                     NSPQQNPSIRGGLTYVIP"
     gene            2627..3886
                     /locus_tag="PLI16_06090"
     CDS             2627..3886
                     /locus_tag="PLI16_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOH84167.1"
                     /translation="MIDNPSSERKFGKFPWKYAESFLVSCSLVFIGFIIEFATGGKGV
                     RLPGFPANLLLLSLFIVYIAIFHFLVKHPVKKWLCSVHAAISAISVFAVLILLMGIVP
                     QLPNKGNDLIDKLGISHIVQSWPFFISCTYLLFVLSFIIVKRAFPLTLKNIAFTFNHL
                     GLWIVLAAGSLGASDTMEFTMMLTKGKETYVAYDQRGKAYDVGFRLELISFSIDEYPP
                     ELGLFDYKTGKSADAKATRPIVKKGGTAIMGDWKIEISDFIENAQKDSTGYLPAVNPG
                     APAAYVKAVHRLNKAVVNGWVSSGSPFVYRDDLQLSDNKFIAMTLRKPRKYISDVMAF
                     YDGDQKTNFQLTVNNPYKFKGWTIYQSGYDEQMGKWSEISIIRLVRDPWLPVVYTGIF
                     MLLAGALLMLWTGRSGIIKNKPQYDLG"
     gene            3873..4664
                     /gene="ccsA"
                     /locus_tag="PLI16_06095"
     CDS             3873..4664
                     /gene="ccsA"
                     /locus_tag="PLI16_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOH84168.1"
                     /translation="MTWADFPYFVILCCVLWLLALVLLMGSGKTTEKISITLVLAGTL
                     SLGIFITGLWISLERPVFRTLGETRLWYSVLLSLISLIIYYRWKYKWLLSYCIIMAMV
                     FLIINYFNPENFDKTLMPALQSPWFIPHVIVYMISYAFLAAASFFGIKGLYFSYKLKP
                     VEDTMRLTDNIVYTGFAFLTIGMLFGALWAKVAWGSYWTWDPKETWALLTWLIYLLYI
                     HFRINKSNNIKLALWILAVSFFVLLICWFGLNYLPSARGSVHVYS"
BASE COUNT         1332 a         1010 c          986 g         1379 t
ORIGIN      
        1 ggccgtattt gtagccgtaa gtactttatt aacatcggga ctgccgccgg cgatagtcac
       61 agtgccggtt actttggcat tgccattaaa ataccctgcc catgttgtat taagatcgtc
      121 tttaaatgcc agggccccaa ataatgtacc taatgcattg ccgcctatga cacctgcaga
      181 atttgaccaa tccaggtaat tataacctgc aacaccggca gtaaaattgt ttccataaaa
      241 tgaataacct ttgatggcag catcaactcc ggtccgtgcc catgaagacc caccatctgt
      301 tgctacagag gaacttccat aacgataaac atatatgccc gttgaatccg gtccaaaatt
      361 gccagaaggc cggtaaacat aaagtttttt cccaacctga ggtaccgaat tgatgccgat
      421 atttgtacca ttattagtca ggatactgtt tgccacccaa tttgttccat tgtgccttaa
      481 tgtctgcatg ttggtccctg tcaccataac gcccgtggct cccgtaggac ctaccggacc
      541 cgtagggccg acgggaccga caagacctgt aggaccagct ggaccgtaag gaccagtagg
      601 gcctattggg ccgggaattc cagcagggcc agtggggccc attgggccgg tggggcctgc
      661 aacgcctggc acattagcct ggttggcata aagtgcatag gggacactta acaactgcgg
      721 agcacctaca ctgctgtatc ccgatccggt gttaacttca atttggaggt atttggcgcc
      781 agtgccccag gcaatggttg caaaagttcc ggacaccacg gtgccagcgc caacggccac
      841 attaaccaac ccaaaattat ttgtggtaac ggcatgtgtc tcctgataca aaaccgcccc
      901 tgtggcactc aggtcgtgta tggtcattcg taacgtcacg gcctgattgg caatgatatt
      961 acctgaaaga tcgcggcata ctgcctgata attgaatttc tgaggcgcct gcccgtaact
     1021 ttcactaact gataggacag ccaacataaa agctactgct aaaaagcata atgttctttt
     1081 cataggataa gaatttaatg attattgatt gatttttatt acgttaaatg tctcgagcgc
     1141 cttattctga aaaagcaggc gtacaaaata atttcctgtg gcatacccgt cgaaatccat
     1201 cattaaggtc aagggcgtgc cgctttctgc tatctgcatg ttttcaacaa gcatttgccc
     1261 catcatgtcg aaaagctgta ttatataagt tccttgttct ggtttatcgt tcacgataaa
     1321 caaatgttcc gttaccgggt tgggataaag ggaccacgcc ggaagttctt cagctgtttc
     1381 cggcagagag gtcatgagga aaaatggttg ctgaaatccc tgtgtcagta ctattgaagc
     1441 gctggaagcc gtaataataa caggttctcc aatggtatag tccatctgga agttgtttat
     1501 attatctaaa taggtaccac cagctccaga caacacatag cgttccgttg attgtgcaat
     1561 taatgatgct gcaagcatcg ttaaaagtgt aaaaagtaat agctttttca tataacaatg
     1621 ttttatttca aattttttgt aaaaataata caaaatcaat gtgaaaccaa gtattttaac
     1681 attattatta attattactt ttattattaa atataacttt tataaaaaca acaatttagc
     1741 aggcatacga aacactgttc agcatctgac gaatttcgat cgtctcacac aaagagtgaa
     1801 tcatgctatt tcgaatttca agacaaaaat aaaaggcctc acgccgattc aaaaaaagaa
     1861 aaaaaaagcc ccgaaaaaat tccggggcca atactgaagc aatgttgttg tttctatgga
     1921 ataacatagg ttaacccacc tctgatagat ggattttgtt ggggcgaatt ggcgccaata
     1981 tcaatatcaa gaaaaggatc gtaaagcaac atggttttta tccaaaggtt gccgctcagt
     2041 ttatgttcgt accccacgcc aatgggacaa cccataaccc acactccttt tttgtatata
     2101 tcgccgaggc tgttttgcag ataccctgaa ttggtaatat gaaactctgc atggccaaat
     2161 attcctttcc acaccgaata ttccacaaaa acagctcctc cgtatgaaaa ttgcccggcc
     2221 atacctgttg ttgaagttgc ctgatactgg ataatcggtg atacgcccac actgaagttt
     2281 ttataacggt aacccgcaat tggagcaaca ttcaggttta atccacctaa aatgccaata
     2341 tttatatcgc cgcctgtaaa aaactgtgca tgtgcacgct caccgaaacc agaaatacag
     2401 aaaaaaaatg cgacaacggt gatgactaat ttccttgctt ttgttttcat agtaattttt
     2461 ttgtgcattg caggcaaaaa taatgccttt ttgagaaaaa tcttaaaatt ttaaatcaga
     2521 atttgtttta cagataaaac aattgtgcgt caggtatcct tacttttgat aaatttgtca
     2581 aacaaaatgc aacaaaaaag aaaggttgga ataatcctca gttattatga ttgacaaccc
     2641 cagttcagaa aggaaatttg gcaagtttcc atggaaatac gccgaaagct ttcttgtttc
     2701 gtgtagtctt gttttcattg gcttcattat tgaatttgcc accggaggca aaggagttcg
     2761 tttacccgga tttccggcca acctgctttt attatcactg ttcatcgtgt acattgccat
     2821 cttccatttt ttggtaaaac atcctgtcaa aaaatggcta tgcagtgtgc acgcggccat
     2881 atcggctatt agtgtttttg cagtcttgat attgcttatg ggtattgtcc ctcagttacc
     2941 aaacaaagga aatgacctca ttgacaagtt gggaatttca cacatagtgc aaagctggcc
     3001 tttctttatc agttgcactt acctcttatt tgtcctcagc tttatcatcg tcaaacgagc
     3061 ttttcccctg acactgaaaa atattgcttt tacctttaat catctgggac tatggattgt
     3121 attggcagca ggttctctgg gagcttccga cacaatggaa ttcaccatga tgcttacaaa
     3181 aggcaaagaa acttatgttg cctacgatca gcgtggtaag gcctatgacg tgggttttcg
     3241 attagagctc atcagtttca gcatagatga atatcctcct gagttaggct tgttcgatta
     3301 caaaaccggt aaatcggcag atgccaaagc tacccgcccc atagttaaaa aaggtggcac
     3361 agcaatcatg ggcgactgga agattgagat aagcgacttt attgaaaatg cccaaaaaga
     3421 ttccacgggg tatttgcctg ctgttaatcc gggggcaccg gcagcatacg taaaagcggt
     3481 gcacaggttg aacaaagccg tagtgaacgg atgggtaagt tccggcagtc cttttgttta
     3541 ccgcgatgat ttacaactat ctgacaataa atttattgct atgacattac gcaagcccag
     3601 gaaatatatt tcggatgtaa tggcttttta tgatggagat cagaagacga attttcagtt
     3661 aacggtgaat aatccatata aattcaaggg gtggactatc tatcagagcg gctacgacga
     3721 gcagatgggc aaatggtcag agataagcat aatccggttg gttagagatc cctggcttcc
     3781 cgtggtttac accgggatat ttatgttgct ggccggcgcc ttgctcatgc tgtggaccgg
     3841 cagatcagga attataaaaa acaaaccgca atatgacctg ggctgatttt ccatattttg
     3901 ttattctatg ttgcgtgtta tggctgctgg cattagtatt gctgatgggg tcaggaaaaa
     3961 caaccgaaaa gatttccatc actcttgtac ttgccggaac gctgtcgctg ggtatcttca
     4021 tcaccggttt atggattagc ctggaacggc ctgttttcag gactttgggc gaaaccaggc
     4081 tatggtactc cgtgttactt tcgttgataa gcctgataat atattaccgc tggaaataca
     4141 aatggctgtt gtcctactgc attattatgg ccatggtatt tctcatcatc aactatttca
     4201 atccggaaaa ttttgacaaa accttgatgc cagcccttca aagcccatgg tttattcctc
     4261 acgttattgt ttatatgatt agttatgcat ttttggctgc cgcatccttt tttggcatca
     4321 aaggcctcta cttttcttat aaactgaaac cagttgaaga caccatgcgg ctgacagata
     4381 acatcgtcta taccggattt gcatttctta ccatcgggat gctgtttgga gccttatggg
     4441 ccaaagttgc ctggggaagc tactggacat gggacccaaa agaaacctgg gcgctgctga
     4501 cctggctgat ttatctatta tatattcatt tcaggattaa taaaagcaac aacataaaac
     4561 tggcactttg gattctggcg gtatcatttt ttgtgttgct tatctgttgg ttcgggttga
     4621 actatcttcc ctccgccagg ggtagtgttc atgtatattc ataagctaat gccttcaaat
     4681 attttttctt ccggactttc tttgggt
//
LOCUS       DAOSFP010000173         4680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16382_length_4680_cov_3.416216, whole genome shotgun sequence.
ACCESSION   DAOSFP010000173 DAOSFP010000000
VERSION     DAOSFP010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16382_length_4680_cov_3.416216"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLI16_06100"
     CDS             <1..382
                     /locus_tag="PLI16_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84169.1"
                     /translation="QFNTVYLTDFDDRDFSSFRKLMSIISPFDAKVHCVHIQQEKSKI
                     NKIMLDELTAHIKSVYSNYDVSCKLINSSEVTKSINEFVKANEINLISLSERKRNWLV
                     KLFSKSVVKDILFKVNTPLLVFKY"
     gene            complement(383..1681)
                     /locus_tag="PLI16_06105"
     CDS             complement(383..1681)
                     /locus_tag="PLI16_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84170.1"
                     /translation="MAEKFPFSMHDAEHILSSTDHIVPGMSRVVYVYYSAIHRQIRSV
                     EALPPYGDPKDLRPFFLDEKDIQAIKKMREQKEDFQWYAEKELTFLCGNEHSSSDDIP
                     KIEAAKNKKNLPPVKKDAGQTSTDEESLRHVLMITIRNENDNNNDLFFYFYNTNLSNY
                     HLSNSTDALVHYDKRVIGLKMYNMINLLVETSRNYRIKLKNTITDLKSKNEALKKSLA
                     ESEQQNELYHKSVVDYAHEILHRLSSESQQYVFELSDDAVSKFKGFKGNLHNLRKKLK
                     EITDNKVLQNIDQHAGNDFEAALTWEFENEKNKQTIFIEASDLSFDSLTEQAIVAKTV
                     KTDGSITSTIKLFDKLEEASGIVKTEGLPLTAYNVGQKCRRKMSPPAISFALKTHGQW
                     LVQVLEEYKDKWPIIRNEFKPLINAIIQAQTEKAQKVKQA"
     gene            1918..2178
                     /locus_tag="PLI16_06110"
     CDS             1918..2178
                     /locus_tag="PLI16_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84171.1"
                     /translation="MEIYSPKKGQVTKFSRNVLKADVEKEKQKRLLQEETLNLKFEKK
                     TTGKAKVEKNIVRNEKKDGLLKKALNSFYGYVSVFFDSEEVV"
     gene            complement(2535..3290)
                     /locus_tag="PLI16_06115"
     CDS             complement(2535..3290)
                     /locus_tag="PLI16_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HOH84172.1"
                     /translation="MLNQYTFNIALVVWILIGLIVFGVLFKVTAPYGKFFSKKWGALV
                     NNRLGWVVMEAPPLLLFSFFFFWGYNDTNLVCLAIYAFWMVHYLHRDLIFPFRLKTRG
                     KKMPAALMSFGLIYNVFNAFFNGYYLGFYADYDISWFTDYRFVIGAIIFIAGFIINIQ
                     SDNILFKLRKETVDYKIPYGGLFRFVSCPNYLGEIMEWLGFAILSWSLAGFSFFVWTT
                     VNLLPRAFSHHEWYRKNFPEYPANRKAIVPFII"
     gene            complement(3302..4276)
                     /locus_tag="PLI16_06120"
     CDS             complement(3302..4276)
                     /locus_tag="PLI16_06120"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOH84173.1"
                     /translation="MKVAVTGASGHVGANLCPLLIKQGFEVNVLSFRDDRAFRHLPLQ
                     IFKGDLNDAASLDGFIAGADVVINTAAYISVNLNDDETVYNTNVNGTKTLIDKCREHH
                     VKRLIHFSSIHAFNPFPMDECLDENRPLAENTEFIYDRSKADAEKIVRAANCSELETV
                     VLNPTSIIGPGDYKPSLIGRTIINLYHKRLPALVSGGYDFVDVRDVAQAAINAISMAK
                     AGEKYLLSGKWHCIKEFGDIFSSASGVGRPWFICPLWLAQASVPLVRPFLNKEIKVVF
                     NRQTIKILKESNRHISSAKAKKELGFTSRDVHESIRDAVAWFKENNYL"
BASE COUNT         1365 a          989 c          961 g         1365 t
ORIGIN      
        1 ccaattcaac accgtgtacc ttaccgattt cgatgaccgt gatttttcat cgttccgcaa
       61 actgatgagc attatcagtc cctttgatgc taaggtgcat tgtgtgcaca ttcagcagga
      121 aaaaagcaaa atcaacaaga ttatgctaga cgagcttacc gcacatatca aaagtgttta
      181 cagcaactat gacgtaagtt gcaagctaat caacagcagt gaggtaacca aaagcatcaa
      241 cgaatttgta aaagccaacg aaatcaacct catcagtctg agcgaacgca aacgcaactg
      301 gctggtcaaa cttttcagca aaagcgtggt gaaggatata ctttttaaag tgaatactcc
      361 cttgctagtt tttaaatact aactatgcct gtttgacttt ttgggctttt tccgtctgag
      421 cttggataat agcattaatc aggggtttaa actcattgcg gatgattggc cacttgtctt
      481 tgtattcttc aaggacctgc acgagccact gaccatgtgt tttaagcgca aaagaaatgg
      541 ccggtggcga catttttcgc ctgcattttt gtcccacatt ataggccgta agaggcaggc
      601 cttccgtttt aacaattcct gaagcttctt ccagcttgtc gaagagcttg atggtgctcg
      661 taatcgaccc gtccgttttt actgtctttg caacaatggc ttgttcggtc aaagaatcaa
      721 aactgaggtc gctggcttca ataaatatgg tttgcttgtt tttctcgttt tcaaattccc
      781 aggtcaaggc agcctcaaag tcattaccgg catgctgatc aatattttgc aaaaccttgt
      841 tatcggtaat ctctttcagc tttttccgca ggttgtgcag attgccttta aatcccttga
      901 atttgctgac ggcatcgtct gataattcaa atacatactg ctggctttcg gatgataacc
      961 tgtggagtat ctcatgtgca taatccacca ccgatttatg gtagagttca ttttgctgtt
     1021 cgctttctgc cagggatttc tttagggctt cgtttttgct tttcaggtcg gtaatcgtat
     1081 ttttcagctt gatgcgatag ttgcggctgg tttctaccaa caggtttatc atgttgtaca
     1141 ttttcagtcc gataacccgc ttgtcataat gtaccagcgc atccgttgaa ttgctcaggt
     1201 ggtaattact caggtttgtg ttgtaaaaat aaaagaacag atcgttgttg ttatcgtttt
     1261 cgtttctgat ggttatcatc aggacatgac gcagcgattc ttcgtcggtg gaagtctgac
     1321 cggcatcttt tttcaccggg ggcaagtttt ttttattttt tgcagcttct attttgggta
     1381 tgtcgtctga agaggagtgt tcatttccgc ataaaaatgt caactctttt tcggcatacc
     1441 actggaaatc ttctttttgt tccctcatct ttttgattgc ctggatgtct ttttcgtcaa
     1501 gaaaaaacgg tctgaggtct ttggggtcgc catagggtgg gagggcttcc acgcttctta
     1561 tttggcggtg tatggccgaa taatacacgt acacaaccct gctcatgccc ggcacaatat
     1621 ggtcggttga gctgaggata tgctcggcat catgcatcga aaacggaaac ttttcggcca
     1681 ttatgaaaat tttaaggcac aaatataata caacaaaatt tattatgcaa atttttaaga
     1741 aaataaatta aggacttaag gtgattaatt tataaattat cttaactatt tccgattttt
     1801 aagttaaggg aaaaatacgt aattaattga aatatagttt gttaaaattg cgttttcata
     1861 aaaaaatgtt gtattattgc atagtgaaaa aataacaaat taataaaagt taaggtcatg
     1921 gaaatatatt cgccaaaaaa aggtcaggta accaaattca gccgcaacgt tttaaaagcc
     1981 gacgtagaaa aagaaaaaca aaaaaggctt ttgcaggagg aaacactcaa cctgaaattt
     2041 gaaaagaaaa ccacgggaaa agcaaaagtt gaaaaaaaca tcgtcaggaa cgagaaaaaa
     2101 gatggtctgt taaagaaagc cttaaattcc ttttatggat atgtaagtgt cttttttgat
     2161 tccgaagaag tggtataaat cagttgctgg attttttctg cctggcaacc atatgaaata
     2221 gtaaaaatac catgagtaat tcctgttcct tgctacgtta ttttatttgt ccggtgtatc
     2281 agccggccac aacaaatccc agcattgtcg ctgtcccggt tccgggtgaa aacactacaa
     2341 agggccaaac gcggccaacc tccgttggga tatctgaaag caagcaaact tgtcctttca
     2401 gatactccct gtgttttcat cacacccatg ccgcggctat tcaggccgca cgggccaaaa
     2461 tgcaaaaaaa tcaggttctt tagtatcgct tgtgttataa ctgcaaaaac cctttaatct
     2521 acaggcaaag cagcctaaat gataaaaggc acaatagctt tgcggttggc cggatattca
     2581 ggaaaattct tccggtacca ttcgtgatgc gaaaaggccc tgggaagtag gttgaccgtg
     2641 gtccatacaa aaaacgaaaa gccggccagc gaccacgaca gtatggcaaa tccaagccat
     2701 tccattattt cgccaaggta attcggacaa gaaacaaaac gaaacagccc gccataaggt
     2761 atcttatagt ccaccgtctc cttgcggagc ttgaagagga tgttgtccga ctggatattg
     2821 ataataaaac cggcaataaa tattatcgcg ccaataacaa aacggtaatc tgtaaaccac
     2881 gaaatatcgt aatcggcata aaatcccagg taatacccat tgaaaaaggc attaaaaaca
     2941 ttgtaaatca atccaaaact catcaaggca gccggcattt ttttgccacg ggttttcagg
     3001 cgaaatggaa aaataaggtc gcggtgcagg tagtgtacca tccaaaaggc ataaatagcc
     3061 aggcacacca ggttggtatc gttgtatccc cagaaaaaaa agaatgagaa aagcagcagg
     3121 ggcggggctt ccatcaccac ccatcccagg cggttattga ccagcgcgcc ccattttttg
     3181 ctgaagaatt ttccataggg cgctgttacc ttgaaaagta ccccgaacac gatcagcccg
     3241 atgagtatcc agactaccag agcaatgtta aacgtatatt gattcagcat tacatttctt
     3301 tttataaata attattttct ttaaaccacg ctaccgcatc cctgatgctt tcgtgtacat
     3361 ccctgctggt aaagcccagc tctttttttg ccttggccga agagatgtgc cggttactct
     3421 cttttaaaat tttgatggtt tgcctgttaa acaccacttt gatttccttg ttaagaaaag
     3481 gccttaccaa aggcaccgag gcctgagcca gccagagagg acaaataaac cagggccggc
     3541 ctacaccact cgctgaactg aaaatgtcgc caaactcttt gatacaatgc catttgcccg
     3601 acagcaagta tttttctccg gctttggcca tgctaatggc attaatggct gcctgcgcca
     3661 catcgcgtac atccacaaag tcgtagcctc cgcttaccaa cgcaggaagt cttttgtggt
     3721 acaggttaat aattgtccgg ccgatcaacg acggtttgta gtcaccaggg ccaatgattg
     3781 atgtgggatt cagcaccact gtttccagtt cactgcagtt tgccgcacgt acaattttct
     3841 cggcatcagc tttactgcga tcgtaaataa attcagtgtt ttcggccaga ggtctgtttt
     3901 catcaagaca ttcgtccata gggaacggat tgaacgcatg gatggaacta aaatgaatca
     3961 ggcgtttcac gtgatgttca cggcatttgt caatcagtgt tttagtgccg tttacgttgg
     4021 tattgtaaac cgtctcgtca tcattgaggt taaccgaaat atatgcggct gtattaataa
     4081 ctacatccgc accggcaata aacccatcga gacttgcggc atcattcagg tctcctttga
     4141 aaatctgcag tggaagatgg cgaaacgccc tgtcgtctct gaatgacaat acattaactt
     4201 caaaaccttg tttaataagc aacgggcata aattggctcc gacatgtccg cttgcgcctg
     4261 taacggcaac tttcatcagg tataattttc ggcgaaggta aatatttttt tcttgcaagg
     4321 cagggtaaaa gtttctggaa ttcaatcgtt tttttgaggt cataactttc attgtcagtt
     4381 ggaataatca caacactaac acctttgcaa aacagccttt atattgcgaa aattatagcc
     4441 taaatccgga ctgttagttt tgacggacca ccgcgccgct attaacaagc atttgtgctg
     4501 tgtgcaaaca tcttatcggt ctatgattca gaaaaacctt tattactaaa agtggcataa
     4561 tgagaaactg atgttaaaaa cttaactacc caagccttct atttctcaat gaataaaggc
     4621 tataaaaaac gataagtatt tgctaccagg caaaatgtta gttagaatgg tcgaattgct
//
LOCUS       DAOSFP010000174         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16489_length_4656_cov_3.884590, whole genome shotgun sequence.
ACCESSION   DAOSFP010000174 DAOSFP010000000
VERSION     DAOSFP010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16489_length_4656_cov_3.884590"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLI16_06125"
     CDS             <1..370
                     /locus_tag="PLI16_06125"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOH84174.1"
                     /translation="EIDSELDLRQINPNFYKVIKQFSPFGPGNMAPVFKTNGVVDLGF
                     ARLVGKNEKHHLKLSITYPEISLPPYPAIAFQLADFFEYINQGNRFDICYHIEENEWN
                     GKTSLQLNIKDIKKSVPEMN"
     gene            complement(380..1261)
                     /locus_tag="PLI16_06130"
     CDS             complement(380..1261)
                     /locus_tag="PLI16_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH84175.1"
                     /translation="MKKFLSKILSYFLFFHLLLLSLLPFWILYRLSDILAFLLFYVIR
                     YRRKVVFKNLEGSLPEKYKTRTVAWQFYRFLADVFLESVKCFTIRKKNLLRRIQCDNP
                     EIMEHYAREHRSVIFMSAHYCNWEYLIYSLNLLFPHWAIGVGKPLSNEVMNKLTNGRR
                     SRFGMKIINAKNIKEEFYKDRNELTASLFLSDQYPGGSHKGVHALFLNRETDFLYGAE
                     KYAVDYDFPVVYADFKRTRRGYYTIHLIKITDTPRQCEYGYIMRTYIDLLEKTILRDP
                     AWWLWSHKRWKQIQGFY"
     gene            complement(1298..2026)
                     /locus_tag="PLI16_06135"
     CDS             complement(1298..2026)
                     /locus_tag="PLI16_06135"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOH84176.1"
                     /translation="MKVYDQIMQKVAGGQKQLSILIDPDNMPQAQVKNIAEAAADAGI
                     DYFFIGGSLITNDNLDKSIETLKRFSSIPVVIFPGNTMQINSRADALLFLSLISGRNA
                     EMLIGKHVIAAPYIRNARLEVISTGYMLIESGKPTSVAYMSNSLPIPRDKDDIAVCTA
                     MAGEMLGLKMIYMDAGSGAKETISPAMIEKVKKNIAVPLIAGGGINSAEKAKSVFDSG
                     ADMIVVGNAVEKNLTLIRQLADAR"
     gene            complement(2112..2750)
                     /locus_tag="PLI16_06140"
     CDS             complement(2112..2750)
                     /locus_tag="PLI16_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOH84177.1"
                     /translation="MPIIIDNQPDKKTRLGVWQITETVEQLEASVFLSEKEKAVLTTF
                     KHDKSKKHWLSYRALIRQLCGDAFSVQYSDFGKPYLHNEEQSQHISITHSGDFSAVII
                     NPDTSVGIDIEEVSRRIERVAPRFLSEKELVFIDRNNFLEHLTICWTAKEALFKISGN
                     DYYDFREQINLLPFGFSRKGVLRAVLNGESGMMELQVYFEKIEEYYLSFVVA"
     gene            complement(2806..>4656)
                     /gene="recG"
                     /locus_tag="PLI16_06145"
     CDS             complement(2806..>4656)
                     /gene="recG"
                     /locus_tag="PLI16_06145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOH84178.1"
                     /translation="LADETGEMELVWFNGVKWLKDKVQAGQKFVVFGKPTYFNGKLNI
                     THPEIEPLTEEFAREEKRRFYPVYSSTEKLKSKGLDSKGISKIVLKALEDARGRIADN
                     LPYDMLNRLKLVSREEAIFNIHYPADQPAINRATARLKFEELFFIQLRLVKTKFYRNK
                     KIPGLVFQNVGKYLNDFYHLVLPYELTGAQKRVVREIRKDLGSGKQMNRLLQGDVGSG
                     KTVVALMSMLIALDNGFQACIMAPTEILSVQHYNTIKNFCSKLAIKVSLLTGSTKSAE
                     RKKILESLLNGETRILLGTHALIEEVVRFQNLGLAVIDEQHRFGVAQRAKLWEKSDRP
                     PHILVMTATPIPRTLAMTLYGDLDVSVIDELPPGRKAIKTLHFGDSNRLKVFAFLKEQ
                     IKQGRQVYVVYPLIHESETLDLKHLMEGYDAVVRDFPLPEYAVSMVHGKMKPKEKEYE
                     MARFVKGETQIMVATTVIEVGVDVPNASVMLIENSERFGLSQLHQLRGRVGRGADQSF
                     CLLMTSDKLTSDARQRIKAMLETSDGFRIAELDLKLRGPGNIEGLEQSGITDLHIADL
                     VADEKILKYARNEAFALLEADPELESPANAAVRQFIVRENKRKGSWGMIS"
BASE COUNT         1234 a         1158 c          939 g         1325 t
ORIGIN      
        1 ggaaattgat tcggaactgg acctgcgcca aataaatcct aatttctata aggtaataaa
       61 acagttctct ccttttggcc cgggaaatat ggcacccgta tttaaaacca acggcgtggt
      121 tgaccttggg ttcgctcgcc tggtgggaaa aaacgaaaaa catcatttaa aactttccat
      181 cacgtatccc gaaatatcgc tgccccctta tccggccata gcctttcagt tagccgattt
      241 ctttgagtat atcaaccaag gcaaccgatt tgatatctgc taccatatcg aagaaaacga
      301 atggaacggc aagacttctc tccagctaaa cattaaagat attaaaaaat cagtaccgga
      361 aatgaactga caatctttgt cagtaaaatc cctgtatttg tttccatcgt ttatgcgacc
      421 acagccacca ggcagggtca cgaagaatgg ttttttcgag caggtcgata taagttctca
      481 taatatagcc gtattcgcat tgacggggcg tatcggtgat ttttatcaga tgaatggtgt
      541 aatacccccg ccgggtacgc ttaaaatcgg cataaacaac aggaaaatca taatctacgg
      601 catatttctc ggccccgtat aaaaaatccg tttccctgtt caaaaacaaa gcgtgcacac
      661 ccttgtgcga gccgccggga tattggtccg aaagaaaaag acttgcggta agttcgttcc
      721 tgtctttata aaactcctct ttaatgtttt tggcattgat aattttcata ccaaagcggc
      781 tgcgccgccc gttggtgagc ttattcatca cttcattcga caggggcttc cccacaccaa
      841 tggcccaatg cgggaaaaga agattaagcg aataaatcag gtattcccaa ttgcaatagt
      901 gtgccgacat aaaaatcaca ctccggtgtt cacgtgcata gtgctccatt atctcaggat
      961 tatcgcattg aatccgcctg agaagattct tttttcttat ggtaaaacac ttaacagatt
     1021 ccagaaaaac atcagccaaa aaacgataaa actgccaagc cactgtcctg gttttatatt
     1081 tttccggcag actcccttcg agatttttaa aaaccacctt ccgccggtat ctgatgacgt
     1141 aaaacaacaa aaaagccaat atatccgaca accggtacaa tatccaaaac ggcagcaacg
     1201 acagcaacag caaatgaaaa aacaaaaaat aactaaggat tttcgacaga aattttttca
     1261 tgtttgccgt ctttattaaa acaaaagtat attaaaatta ccgtgcatca gccagctgcc
     1321 gtattagcgt aagatttttt tccaccgcat tacctaccac tatcatatcg gctccggagt
     1381 caaaaactga ctttgccttt tcggcagaat tgatgcctcc cccagcaatt aacggtacag
     1441 caatattctt ttttaccttt tcaatcatgg cagggcttat tgtttcctta gcgccgcttc
     1501 ccgcatccat gtatatcatt tttagtccaa gcatttcacc ggccatggcc gtacacacgg
     1561 cgatgtcgtc tttgtcgcgc ggtatgggca acgagttgct catataagcc accgaggtgg
     1621 gtttcccgct ttcaatcagc atatatccgg tggatatgac ttcaagtctg gcgttacgga
     1681 tatacggcgc cgcaatgaca tgtttcccga taagcatctc ggcgttgcgc cccgagataa
     1741 gcgaaagaaa aagcaacgca tcggcacggg agtttatttg catcgtattt ccgggaaata
     1801 tcaccacagg tattgacgaa aaccttttca gcgtttcgat ggatttatcc agattatcgt
     1861 tggttatcag gcttccgccg ataaaaaaat agtcaatacc cgcatcagca gcggcttcag
     1921 ctatattttt cacctgtgcc tgcggcatgt tgtcgggatc aataagtatg ctgagctgct
     1981 tttgtcctcc ggcaactttc tgcattattt gatcataaac tttcataaga cgaaattaca
     2041 aaataattca acagcatcac ttttcggcaa gcgggaaggt tgcgcgctgg aaaattgcag
     2101 acggcattgg ctcaggccac cacaaaagac aaataatatt cttcaatttt ttcgaaatat
     2161 acctgcaact ccatcatgcc tgattctcca ttaagtacgg ccctgagcac acctttgcgg
     2221 gaaaaaccaa aaggcaacaa atttatctgc tccctgaaat cataataatc attgccgctg
     2281 atcttaaaca gcgcctcttt ggccgtccag cagatggtaa ggtgttcaag aaaattattc
     2341 cggtcaataa agaccagttc tttttccgaa agaaatctgg gggccactct ttcaatccgc
     2401 ctgctcacct cctctatatc gatacctacc gaagtatccg gatttattat gaccgccgaa
     2461 aaatctccgg aatgagtaat tgagatatgc tgactttgtt cttcattatg cagatagggc
     2521 ttcccaaaat cgctatattg tacgctaaag gcatcaccgc aaagttgacg tatcagtgcc
     2581 cggtaactca gccagtgctt tttactttta tcatgtttga aggtagtcag gactgctttt
     2641 tctttttccg acaaaaacac agatgcctcc agttgctcca ccgtttcggt gatttgccat
     2701 acacccagac gggttttttt atcaggttga ttgtctatta ttattggcat agataatttt
     2761 caaaaacagt ttttcagcac gaaagcaata ctacatccac cttttttagc ttatcatccc
     2821 ccaggagcct ttgcgtttgt tttcccttac tatgaattgc ctcaccgccg cattagccgg
     2881 cgattccagc tcgggatctg cctccagcaa tgcaaaggct tcgttacggg catattttaa
     2941 aattttttca tccgccacca ggtcggcaat atgcagatcg gttatgccgc tttgctccaa
     3001 gccttcgata ttgccaggcc ccctgagttt caggtccaat tcggcaatac ggaagccatc
     3061 gctggtttca agcatcgcct ttattcgctg gcgggcatcg ctagtaagtt tgtcggaagt
     3121 catcagcagg cagaacgact ggtcggcgcc tctgcccacc ctgccacgca actggtgaag
     3181 ctgcgacaag ccaaaacgtt cagaattttc aattagcatc accgaggcat tggggacatc
     3241 cacaccgact tctatcaccg tggtggctac cattatttgt gtctcgcctt tgacaaagcg
     3301 tgccatctca tactcttttt ctttgggttt catcttgccg tgtaccatgc tcactgcata
     3361 ttccggcaaa ggaaaatcac gcaccacggc atcataacct tccatcaggt gcttcaaatc
     3421 aagggtttcc gattcgtgta tcaacggata gacaacatat acctgccggc cttgttttat
     3481 ttgctctttc agaaaggcaa aaactttcag gcggttggaa tccccgaaat gaagggtttt
     3541 tatcgccttt cttccgggag gcaactcatc aatcacagaa acatcgaggt cgccgtatag
     3601 tgtcatggcc agcgtgcgcg gaataggcgt ggcggtcatt accaggatgt gcggcggcct
     3661 gtcgcttttt tcccaaagtt tggctcgttg tgccacacca aagcgatgct gttcgtcgat
     3721 caccgccagg cccagattct gaaacctgac aacctcttca atcagggcgt gggtacccag
     3781 caaaatcctg gtttctccgt ttagcaggct ttccagtatc ttttttcgtt ctgcactttt
     3841 ggtggaacct gtcaacaggc tgactttgat agccagtttg ctgcaaaagt tttttatcgt
     3901 gttataatgc tggaccgaaa gaatttcggt gggcgccatg atacatgcct gaaacccatt
     3961 gtcgagggct atcagcatac tcatcaatgc caccacggtc ttgccgctgc ccacatcgcc
     4021 ctgcaataaa cggttcattt gcttgcccga acccaggtct ttcctgattt ccctgacaac
     4081 tcttttttga gcgccggtta gttcgtaagg caacacaaga tgataaaaat cgttcagata
     4141 cttccccaca ttttgaaata ccaatcccgg gatttttttg ttccggtaaa attttgtttt
     4201 taccagccgc aactggataa aaaacaattc ttcgaatttc agccgtgcgg tagcccggtt
     4261 tatggcaggt tgatctgccg ggtaatgtat gttaaagatg gcttcttctc ttgaaactaa
     4321 tttcaatcga ttcagcatat cataaggcag gttatcagca attctccccc gggcatcctc
     4381 cagtgccttt aacactattt tgctaatccc tttactgtct aggcctttgg atttaagttt
     4441 ttcggtggaa gaatataccg gataaaaccg ccgtttttct tccctggcga actcttctgt
     4501 caggggttca atttcaggat gggtaatatt cagtttgccg ttgaagtagg taggtttgcc
     4561 gaacaccaca aatttctggc cggcctgcac tttgtctttc agccacttta caccattaaa
     4621 ccacaccagt tccatctcac cggtttcatc ggccag
//
LOCUS       DAOSFP010000175         4651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16512_length_4651_cov_3.616188, whole genome shotgun sequence.
ACCESSION   DAOSFP010000175 DAOSFP010000000
VERSION     DAOSFP010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16512_length_4651_cov_3.616188"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="PLI16_06150"
     CDS             complement(<1..1228)
                     /locus_tag="PLI16_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH84179.1"
                     /translation="MKKILSLLFFVVITTSAFSQQVCQANFTYSYTPGSNTLYLYDAS
                     YNVDSTQINVTTWTWTVQYSGMSMTYNTQNPVIAFNNFPGTVNVCLNIGTSGLCQSSY
                     CNTITLQNTNCYTHFTYSATTNYTYSFNGYSFVAGSLVTSSNYVWNFGDGNSANTQNT
                     THTYSQPGTYIVCLNTTTVANTCTSTFCDTIIVQDTGCIMNVSAVITNVSVSGGSDGA
                     IVLTVSGGTPPYNFAWSNGATTQNLTNIPAGTYTVHITSSNPACPGATYTFQVLQPNT
                     LCQAYFTYTIDSVLNTVNLSDQSYNGNSSQINTSSWYWTVSTGQNLVFTSTQPNPSFP
                     LSPTMIYAYVCLTITTASGCTSTYCDTIYLQGTPTVNCNAYFSYTQTGLNAFEFFGHM
                     VTPGATANVILWYWDFG"
     gene            complement(1292..2905)
                     /locus_tag="PLI16_06155"
     CDS             complement(1292..2905)
                     /locus_tag="PLI16_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84180.1"
                     /translation="MKSPEKNFDELIKQQLSDIEIQPPAPLKKQVFGKLAMLAFFRYI
                     KRNSLFVLLLLMISVLGIFSYNASRETGSDPKENNGYKSSSYKTDNENDNTAAQKNQD
                     ITFDEKKSDATHNENTTHNSNSNTNNNTQDLADNTTGSEKNIQPETEHSAAKTAVAPA
                     INNSQKRHDDNLTSNLATSASGTPTEILPEYENNDHTQGNNLAINKSGTALQETSNAA
                     ASGVQEINKTTGYSPNVRTEFAISEMLTLPGLIDTENSLKVSYPPAPVNKMRQPLSWS
                     VGMNIGHSLLQYPLTRLPENRDDDTYSYSSTLYYPSITAGIDIRAEKRHLFFEAGVQY
                     ALFTENIKSDNVLINPQNHQFYNFTGQTMNVDTGGGSYHYFWICDSVPRLLDSVWTWK
                     IDTTYLNLYDTVNTKVYDTLHHPEWRNSYTFIELPLSVGWQYNFGRLNVGIKTGPIMS
                     LLVGTKGFVPSGRNEQAYMNTLDSEFKKFSMGLSWHIATMVSYLINERILLECQPYYR
                     MNLKSISTSSGYAMKNNSLGITVGVRYFF"
     gene            complement(2902..3489)
                     /locus_tag="PLI16_06160"
     CDS             complement(2902..3489)
                     /locus_tag="PLI16_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH84181.1"
                     /translation="MEIIELIDGCRKWHKKAQNELYDKYAPRLFAVSLRYVADRDLAR
                     DVLQEALIKIYKNIGSFTLTDDKAFYCWMHRITVNTALNHLRKDLKNKLNTEIQNFEN
                     EISNDTYEESFSVYDEIIACIGSRALFTMVGELPDGYRTVFNLYVVEGFSHKEIAEQL
                     DITINTSKTQLFKARKMLMSKINGIVKNNTLKEVV"
     gene            complement(3682..>4651)
                     /locus_tag="PLI16_06165"
     CDS             complement(3682..>4651)
                     /locus_tag="PLI16_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH84182.1"
                     /translation="KKRLLRTPLDPDITFSDDPLRMLRAIRFAAQLNFSIWPETFEAI
                     TKNKERIHIISQERIIAELNKIVLSPVPSIGFDLLDQSGLLEIIFPQFAALKGVEIVD
                     NKGHKDNFYHTLEVLDKISLNSDNLWLRWAALLHDIAKPVVKKYVPATGWTFHGHEAK
                     GSQMVYEIFKMLKLPLNEKMKYVQNLVFLHLRPIALVEETVTDSAVRRLLFEAGDNID
                     DLMMLCEADITSKNIKKVERYLKNFEIVRQKLAEIEEKDRLRNWQPPISGEIIMETFG
                     LQACKEVGIIKTAIREAILDGTIGNNFDEAFQFMLQQGEKLNLKKK"
BASE COUNT         1148 a          912 c         1133 g         1458 t
ORIGIN      
        1 cgccaaaatc ccaataccac aatatcacat tggcggttgc tcccggagtt accatgtgtc
       61 cgaaaaattc aaaggcattc agccccgttt gcgtgtaaga aaaatatgcg ttgcaattta
      121 ccgtgggagt tccctgcagg tatatggtgt cgcaataggt actggtacag cccgatgctg
      181 tggtgatagt aaggcacaca taggcataga tcatagtggg gcttaggggg aaactcggat
      241 tgggctgcgt ggatgtaaac acaagatttt gtccggtact cactgtccag taccagctgg
      301 aggtgtttat ttgtgaactg ttgccattgt aggactggtc ggaaaggttg actgtattga
      361 gcacactgtc aatagtatag gtaaaatacg cctggcagag tgtgttgggt tgcagcacct
      421 gaaaggtgta agtggcaccc gggcaggcag ggttgctgct ggtgatatga accgtgtagg
      481 ttcccgccgg tatattggtg aggttttgtg ttgtggcgcc gttcgaccag gcaaagttgt
      541 aaggaggcgt tccgccgcta actgtcagca ctatagcacc atcgctgcct cccgaaacgg
      601 aaacattggt gatgacagcg cttacattca tgatgcaacc ggtatcctgc acgatgatgg
      661 tatcgcagaa tgtagatgta caggtgttgg cgactgtggt ggtgttcaga caaacgatat
      721 aggtccccgg ctggctatag gtatgtgtgg tgttctgggt gttggcagaa ttgccgtcgc
      781 caaaattcca tacatagttg ctggatgtta ccaacgaacc ggcgacaaag gaatacccat
      841 tgaagctata ggtgtagttt gtcgttgccg agtaagtgaa atgggtataa caatttgtgt
      901 tttgcaaagt gatagtgttg cagtacgaac tctgacaaag gccggaggtg ccgatattga
      961 ggcatacatt aacagtaccg gggaagttgt taaaggcaat taccggattt tgtgtgttat
     1021 aagtcatgga catgccgcta tattgtacgg tccaggtcca tgtggtgaca ttaatctggg
     1081 tagagtccac attgtatgaa gcatcataca gatacagggt gttactgcct ggcgtgtagc
     1141 tgtatgtgaa attagcctgg caaacctgtt gcgaaaatgc acttgttgtt ataacaacaa
     1201 agaacaggag tgatagaatt tttttcatgg ttatggattt ttggggtttt atattaaaaa
     1261 caatttttaa aaaatttggt tgcccgggat gttaaaaaaa atatctaacg cccacggtaa
     1321 tgcccagtga attatttttc atcgcataac cggaggatgt gctgatgctt tttaaattca
     1381 tccggtagta aggttggcat tccaaaagta tgcgttcgtt gattaagtaa ctgaccatcg
     1441 tagcaatatg ccacgaaagt cccatgctga attttttaaa ctccgagtcc agggtattca
     1501 tataggcctg ctcatttcgg cccgatggca caaaaccttt ggtgcctacg agcaaactca
     1561 ttattggacc tgtcttaatg ccgacgttca gccttccaaa attatactgc cagccgaccg
     1621 ataaaggtag ttcgataaag gtgtatgaat ttctccattc cggatggtgc agcgtgtcat
     1681 aaacctttgt gttgaccgta tcgtataggt tgaggtaggt agtgtctatt ttccatgtcc
     1741 agacagaatc caataatctt ggaacagagt cgcaaatcca gaaataatgg taggatccgc
     1801 ctccggtatc tacattcatg gtttgtccgg tgaaattata aaactgatgg ttttgcggat
     1861 taatcaatac attatctgat ttaatatttt cggtaaacag ggcatattgt actcctgctt
     1921 caaaaaacag atgtcttttt tcggccctga tatcaatgcc cgctgtgatt gacggataat
     1981 acagtgtgga tgaataacta taagtatcat catcccggtt ttcggggagc cttgtcaacg
     2041 gatattgcag cagcgaatgg ccgatgttca ttccgacaga ccacgataac ggctgacgca
     2101 ttttgttgac aggtgccgga gggtaagaca ctttcagtga gttttcggta tcaataaggc
     2161 ctggaagtgt gagcatttca gaaattgcaa attccgttct tacatttggt gaatagccag
     2221 tggttttgtt tatctcctga actcctgaag ctgcggcatt tgaagtttcc tgtaatgctg
     2281 tgccagattt attaattgca aggttgttgc cctgggtatg gtcattgttt tcgtattcgg
     2341 gaagtatctc tgttggtgtg ccggaggctg atgtggcaag gtttgaagtc aggttgtcat
     2401 catgtctttt ttgggaattg tttatagcag gggcaacagc agtttttgca gcggaatgct
     2461 cagtttcagg ttggatgttt ttttcagaac ctgttgtgtt atctgctaaa tcctgggtgt
     2521 tgttattggt gttgctgttg ctgttatgag tggtgttttc gttgtgggtt gcatctgatt
     2581 tcttttcatc aaaagtgata tcctgatttt tttgagcggc ggtattatca ttttcattgt
     2641 cagttttgta tgagctgctt ttgtatccat tgttttcttt ggggtcagag cctgtttcac
     2701 ggctggcgtt atagctgaat attcccagga cgctaatcat aagcagtagc aagacgaaca
     2761 ggctgttccg tttgatatat ctgaagaacg ccagcatggc gagctttccg aacacttgct
     2821 tttttagagg agccggcggt tgaatttcta tatcactcaa ctgttgtttt atcaattcgt
     2881 cgaaattttt ttctggactt ttcatactac ttcttttaaa gtattattct tgactattcc
     2941 gttgattttt gacatcagca ttttgcgcgc cttgaacagc tgtgtcttgg aggtgtttat
     3001 tgtgatgtcg agttgttcgg ctatctcttt atggctgaaa ccttccacca catagagatt
     3061 aaacaccgtg cggtaaccat ccggcaactc gccgaccatt gtaaacaatg cacggctgcc
     3121 tatgcaggct atgatttcat cataaaccga aaaactttct tcgtaagtat cgttggagat
     3181 ttcgttttca aagttttgta tctctgtgtt gagcttgttt ttcagatctt tacggaggtg
     3241 gtttaatgcg gtattcacgg taatgcggtg catccagcaa taaaaagcct tgtcgtcggt
     3301 aagagtaaaa gaaccgatat ttttatagat tttaatcagg gcttcttgca gtacatcacg
     3361 ggccagatct ctatcggcca catagcgcag gctcacggca aaaagcctgg gagcatattt
     3421 gtcgtaaagc tcgttttggg cttttttatg ccattttctg catccgtcta tgagttctat
     3481 gatttccatc aaatcactat taacataata acacttttga tgttaaaagg gttgcctgta
     3541 agatattaaa ttttgttatt ctttgtacat tgtctttcca aaaatgagcc aaaagccgcc
     3601 cgggcaggat ggcagactat ctttacctga ttttggcaaa attctataca aaaaaggtaa
     3661 aaaaaattac tggttgaaaa tttacttttt tttcagattt agtttttctc cctgctgaag
     3721 cataaattgg aaagcttcat cgaagttgtt gccaatggtg ccatcgagga tggcttcacg
     3781 tatggccgtt ttgatgatgc caacttcttt gcaggcctgc aggccgaatg tttccatgat
     3841 tatctcgcct gagatgggtg gctgccagtt gcgcaggcgg tctttttcct ctatctcggc
     3901 aagtttttgc ctgacgattt cgaagttttt caggtaacgt tcaacttttt ttatgttttt
     3961 cgaggtgatg tcggcttcgc ataacatcat caggtcgtcg atgttgtcgc cggcttcaaa
     4021 aagcagacgc cgcactgccg aatccgttac ggtttcctct accagcgcga taggccgcag
     4081 gtgcagaaaa accaggttct gcacgtattt cattttttcg ttcaggggca gtttcaacat
     4141 cttgaaaatc tcgtacacca tctgcgagcc tttagcttcg tgtccgtgaa atgtccagcc
     4201 ggtagcgggt acatattttt ttaccaccgg cttggcgata tcgtgcagca aagcagccca
     4261 ccgcagccac aggttgtcgg aattgagcga aattttgtcc aatacctcca gcgtatggta
     4321 aaaattatct ttgtgccctt tgttgtcaac tatttcaaca cctttcagcg cggcaaactg
     4381 cggaaatatg atttcgagca gtccggattg gtccaggagg tcaaacccta tggaaggcac
     4441 aggcgacagc acaattttgt tcagttcggc gatgatacgt tcttgggaaa tgatgtggat
     4501 acgttctttg ttttttgtta tagcttcaaa agtttccggc cagatactga aattcagttg
     4561 tgcggcaaag cgtatggccc gcaacatgcg taacggatcg tccgaaaatg tgatatcggg
     4621 gtctaaaggt gttcgcaaca agcgtttttt t
//
LOCUS       DAOSFP010000176         4620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16670_length_4620_cov_3.485871, whole genome shotgun sequence.
ACCESSION   DAOSFP010000176 DAOSFP010000000
VERSION     DAOSFP010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16670_length_4620_cov_3.485871"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLI16_06170"
     CDS             complement(<1..298)
                     /locus_tag="PLI16_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84183.1"
                     /translation="MKKIYKKISMLIFLFMMGNASFGQPGNIHYGVMGNWGDMALSDR
                     GVTSWASIQNPLPLATTISFLFNTTAGNYTPKWCGSTTDYTRTVNTMLEGSAYYY"
     gene            528..2915
                     /locus_tag="PLI16_06175"
     CDS             528..2915
                     /locus_tag="PLI16_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH84184.1"
                     /translation="MKTYRALAFLFVLLFQLFANGLSGQSRADVREFSGTYGIATPVT
                     LKQDSTVIYLDDFFQDVNVIDSVRAEKCFAVHFTADNKQLVLVNNGNIPVLSELKIWM
                     GATSYSVLLKKSEKKEVSVVFDPEGRVYKAVQLAGDVNGWNPAATTMTYADKLWKATL
                     FLNPGTYYYQLVVDGKWMLDPANPDSADNNIGGFNSVLKVSNQAGIPKPSIVTQSASD
                     NKIIIRKENKVSKLFVFWQNFRLADQSIHEKGNRVEIEIPEAASALERSFIRVFACNE
                     SGSGNDLLIPLHHGKVLTEASGLTRYDKEAQVMYFMLVDRFYDGDPANNFPVKDPEVL
                     PKANYMGGDIAGLKKKIDEGYFSELGVNMLWVSPLNQNPENAFREFPEPHRKYSGYHG
                     YWPVSSTKVDYRYGTDDELKKMVASAHSQDINVILDYVAHHVHSEHFLAKQHPEWFTQ
                     LDLPDGRKNIRLWDECRLTTWFDTFLPTFDFSKPEVVECMSDSALLWITNYDLDGFRH
                     DATKHIPESFQRRLTQKIKNQIIVPKNKSFYQIGETYGNRELVGSYVNSGELDGQFDF
                     NLYFDLRSVLLNENEPFEKLANSLQESLSQYGCHNLMGNISGNHDMARFISYADGSLS
                     SREDDKEAGWSRNIQVQDTAAYQKLSLLTAYIMTIPGIPVLYYGDEIGMPGAGDPDNR
                     RMMKFDNLTNEEKATKSTATRLVKLRRSSMPLIYGDYKVISITKDTYVFVRTYFEKSV
                     IAVFNKSKQPEKIFFKLPSDIANQAFQTNFGHSFIQSENKIEITLPAYKFELLNN"
     gene            2989..4323
                     /locus_tag="PLI16_06180"
     CDS             2989..4323
                     /locus_tag="PLI16_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOH84185.1"
                     /translation="MTFCNTDLVEAQTLVDAPIREKTVPEWSKNLTIYEVNLRQYSSS
                     GSFKDFESHLPRLKEMGVGILWFMPINPIGEKNRKGSLGSYYSVRDYMAVNPEFGTID
                     DFKNLVKKCHQYGMYVLIDWVANHCAWDNELLSRHPDWFMQDSAGNIIAPVPDWTDVA
                     DLNYDNKEMRRYMINAMKFWLTETDIDGFRCDVAGMCPVDFWNEARPALDSVKDVFVL
                     AEWEDPAFHQKAFDMSYTWEFHHLITDIAKGKKTALSLDTLFFNESKKYRAGDYRMYF
                     TSNHDENSWNGTAYEKFGDGVKTFAVLTFMVPGMPLIYSGQEAGLNKRLKFFDKDTIE
                     WKKDEFYDLYKVLTALKKENKALWNGTYGGTMGKIKTSDDAAVYAFCRKKDGDEVVAL
                     FNLSGKPAKVSITESPAEGTFTDVFSGKTVNIKAGDTYKLKAWEYVIAEKRK"
     gene            4425..>4620
                     /locus_tag="PLI16_06185"
     CDS             4425..>4620
                     /locus_tag="PLI16_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84186.1"
                     /translation="MKKLLLSILAISLSIICCGQDKYMPVYFDYLITKQHVDSLLNIN
                     VKEVIIVRTDKAFNELYSEDS"
BASE COUNT         1381 a          912 c         1005 g         1322 t
ORIGIN      
        1 tatagtaata cgcgctgcct tccagcatgg tgttcactgt cctggtataa tccgtggttg
       61 aaccgcacca ttttggcgtg taattccctg ctgtagtgtt aaataaaaat gatatagttg
      121 ttgcaagggg cagggggttc tgaatgcttg cccaagaggt aacccctctg tcactcaatg
      181 ccatgtctcc ccaattgccc atcacaccgt aatgaatgtt tcctggttgg ccgaatgatg
      241 cattgcccat cataaaaagg aaaatgagca tagaaatctt tttgtaaatt tttttcataa
      301 gcaaattttt taatttaagt attaaaataa tttatattta attctatcga tgatattgca
      361 aaattaaaaa atatacttca taatagcaaa ataatttatg tggttaaaat gattatttta
      421 gttatccttt gttattaccg ttttactgac ataaaatgta cctttttcca gcgtaaaaaa
      481 taaaattatt ttgtattttt acgggcttgt taatatccgt taattacatg aagacctaca
      541 gggcgttagc ttttttattt gttctcctgt ttcagttatt tgcgaacggt ctgtctggcc
      601 agtccagggc tgacgtcagg gagttttcgg ggacgtatgg tattgccact ccggttacac
      661 ttaaacagga ttccacagta atttatctgg acgatttttt tcaggatgtc aacgttattg
      721 attccgtacg tgcagaaaaa tgttttgctg tccatttcac cgctgataat aaacagcttg
      781 ttcttgtcaa taatggcaat atacctgtcc tctccgaact gaaaatctgg atgggtgcca
      841 cttcttattc agtgttgctg aaaaagtctg aaaagaaaga agttagtgtg gtatttgacc
      901 ccgaagggag ggtgtacaag gctgttcaac tggccggaga tgtcaacggc tggaatcctg
      961 cagccacaac gatgacttat gcggataaac tctggaaagc cactttgttt ttaaatccgg
     1021 gcacttatta ttatcagctt gtggtggacg gaaaatggat gctcgaccct gccaatcctg
     1081 attcggcgga taataacatc ggcggattta attcggtgct gaaagtcagc aatcaggccg
     1141 gaattcccaa accatcgata gttacacaaa gcgcttcgga caataaaata attatccgga
     1201 aagaaaacaa agtcagcaaa ttatttgttt tctggcaaaa tttcagattg gcggatcagt
     1261 caatccatga aaaaggaaat cgggtggaaa tagaaattcc tgaagctgct tcagcccttg
     1321 aacgttcgtt catccgtgtg ttcgcctgca acgaatcggg cagtggcaac gacttgttga
     1381 taccgcttca tcatggaaaa gtgctcacgg aagcttccgg ccttacccgt tatgacaagg
     1441 aagcacaggt gatgtatttt atgctggtcg accgttttta tgacggcgat ccggccaata
     1501 actttccggt gaaggatccc gaagtgttgc ctaaagccaa ttatatggga ggggatatcg
     1561 ccggactgaa gaaaaaaatt gacgaggggt atttttctga gctgggtgta aatatgctgt
     1621 gggtttcgcc acttaatcag aatcccgaaa acgctttcag ggaatttcct gaaccgcacc
     1681 gcaaatattc aggatatcac ggttactggc ctgtttcttc aacaaaagtt gactaccgtt
     1741 atggaacgga cgatgaactg aaaaagatgg tagcttcagc acatagccag gatattaacg
     1801 tgattcttga ttatgtagct catcatgtcc attcggagca ttttttggca aaacagcatc
     1861 ccgaatggtt tacgcaactt gatctgcccg atggcagaaa aaacattcgg ctgtgggatg
     1921 aatgccggtt gaccacctgg ttcgatacct ttctgcccac ttttgatttt tcaaaacccg
     1981 aagtggtgga atgtatgtcg gactcagcct tgctttggat cactaactac gaccttgatg
     2041 gattcaggca cgatgctacc aagcatattc ctgaaagctt tcaacgccgg ctgacacaaa
     2101 agatcaaaaa tcaaatcatc gtacctaaaa acaaaagttt ttaccagatt ggagaaacat
     2161 atggaaaccg cgaactggtt ggcagctatg tcaattccgg cgaactcgac gggcagttcg
     2221 actttaatct gtatttcgac ttgcgttctg ttcttctcaa cgaaaacgaa ccttttgaaa
     2281 aactcgccaa ctccctgcag gaaagcctga gccaatacgg ttgccataac ctgatgggaa
     2341 acatcagcgg aaaccatgat atggctcgtt ttatctctta tgccgacggc tcactgagct
     2401 cacgggagga tgacaaagaa gcagggtggt cgcgaaatat tcaggtacaa gatactgcag
     2461 catatcaaaa actttcgttg ctgacagctt atatcatgac aatcccgggt atccctgtgc
     2521 tgtattatgg tgatgaaatc ggtatgccag gcgctggtga tcccgataac cgaaggatga
     2581 tgaaatttga caacctcaca aacgaagaga aagcaacaaa aagtaccgcc acccggctgg
     2641 ttaaactacg tagaagcagc atgcccctga tatacggaga ttataaggtt atcagcatta
     2701 ccaaggacac ctatgttttt gtccgcactt actttgaaaa atcagtaatt gctgttttta
     2761 ataaaagtaa gcagccggaa aagatttttt ttaaattgcc atcggatatt gccaatcagg
     2821 catttcaaac taatttcggt cattctttta ttcaatcgga aaataaaatt gaaataaccc
     2881 tgcctgccta taagtttgaa ttattgaata attaataata tagcaatata acaactttat
     2941 atgagaaata cttccaaatt ttttttaata atattaattt tctgttctat gacattttgc
     3001 aacactgatt tggtagaagc acagacgcta gttgatgccc ccattcgtga aaaaaccgtt
     3061 cccgaatggt ccaaaaatct aactatttat gaagtcaatc tcaggcaata ctcttcatcc
     3121 ggatcgttca aggattttga aagccatttg ccgcgcctca aagaaatggg tgtgggcata
     3181 ctgtggttta tgcctataaa tcctatcggc gaaaagaacc gcaagggcag tcttggaagc
     3241 tattattcgg tgcgggatta tatggcggta aaccccgaat ttggaaccat agatgatttt
     3301 aaaaacctgg tgaaaaaatg tcatcagtat ggtatgtatg tacttatcga ttgggttgcc
     3361 aaccattgtg catgggataa cgaacttctc agccggcatc ccgactggtt tatgcaggat
     3421 tcagcaggca atatcattgc cccggtgccc gactggaccg atgtggccga ccttaattac
     3481 gataataaag aaatgcgccg gtatatgatc aatgctatga aattctggct caccgagaca
     3541 gatatcgatg gttttcgttg cgatgtggct ggcatgtgcc cggtagattt ctggaacgaa
     3601 gcccggcctg ccctcgacag tgttaaagat gtgtttgtgc ttgcggaatg ggaagatcct
     3661 gcatttcacc aaaaggcttt tgacatgtca tatacctggg agtttcatca cctgattacc
     3721 gacattgcca aagggaaaaa aacggctttg tcgctggata cgctgttttt caacgaaagc
     3781 aaaaagtacc gtgccggcga ctaccggatg tattttacct ctaaccacga cgaaaattca
     3841 tggaatggta cggcctacga aaaatttggc gacggcgtaa aaacttttgc cgtactcact
     3901 tttatggttc cgggcatgcc cctgatttat agcggtcagg aagcgggact aaacaaacga
     3961 ttgaagtttt ttgacaagga taccatagaa tggaaaaaag atgaattcta tgatctgtac
     4021 aaagtactca ctgccttgaa aaaagaaaac aaggcactgt ggaacgggac ctatggcggc
     4081 acaatgggca aaataaaaac ctcggacgat gccgccgtat atgctttttg caggaaaaaa
     4141 gacggcgatg aagtagttgc tcttttcaac ctctctggta aaccggcaaa agtatcaata
     4201 accgaaagtc ccgcggaagg aacatttacg gatgtgttct caggaaaaac tgtgaacata
     4261 aaggcaggcg atacgtacaa attgaaagcc tgggaatatg taattgcaga aaaaaggaaa
     4321 tagtcgattt gttctaggtt gtaacactac gtaggtgttt cttttgaatt tttctcccgg
     4381 aaatttttga ttgatgagaa aagttagttg ttagcgaata gtttatgaaa aaactactac
     4441 tttccatatt ggcaatttct ttatccataa tttgttgtgg acaggataaa tatatgcctg
     4501 tttatttcga ctatttaatc accaaacagc atgttgacag tttattgaat attaatgtga
     4561 aagaggtcat aattgttaga acggataaag catttaatga actttatagc gaagacagta
//
LOCUS       DAOSFP010000177         4585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16871_length_4585_cov_4.108830, whole genome shotgun sequence.
ACCESSION   DAOSFP010000177 DAOSFP010000000
VERSION     DAOSFP010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16871_length_4585_cov_4.108830"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="PLI16_06190"
     CDS             complement(<1..128)
                     /locus_tag="PLI16_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOH84187.1"
                     /translation="MKWSGRRQSDNVEDRRSISGKGIAGIGGIGAIIIAVIAFFIT"
     gene            542..2518
                     /locus_tag="PLI16_06195"
     CDS             542..2518
                     /locus_tag="PLI16_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOH84188.1"
                     /translation="MIFLLLTVCSFLIFVVPRKAKVYSNLLLNTVLAAYTLLLCGGVF
                     MGAGTYTEVLPVYFWGQQIKLIIDPLSAFFIGIINFTVLTASVYAIGYMKMYRNKSSV
                     ELSIHYLSFFLLHLSMILVCMIHHGIAFLVVWELMAISSFLLVIFDNEKSSVLKAGIN
                     YLIQMHVGALFLIVAFIILNINTGSFDFKALKSFFYNHQNLPLFLLFFVGFGIKAGFM
                     PLHTWLPHAHPAAPTHVSAVMSGVMIKLGIYGILRVAMFLQADILWIGIIVLLISIIS
                     GIGGVSWAIVQHDLKKLLAYHSIENIGIIGIGIGIGLIGLGAENSLLAILGFAGAMLH
                     VLNHSLFKSLLFYGAGAVYLKTHTRNIELLGGLIKKIPVTAILFLIASLAICGLPPFN
                     GFVSEFIIYKGIIVGLNEPSIGVKVLMLAAIISLVLIGGLAVFCFTKAFGVTFLGKSR
                     HVDISGVNELSGLMLAPLFLIAAAILFIGLFPQWAIQPVNFVLESRAFNIAGNSDMMY
                     ALPMIQKIGVAAALFIGLTGLVWLWRFVMTRKKPLTHSGTWGCAYDGAIEKGQYTATS
                     YAENFTDIAGPLVNVKTQYHALHEDDIFPESRSFETFSEDMMEKKLYGQSVGWLNRAL
                     SRLAVIQTGNTQHYILYAFAMILLLLLLTLFNIL"
     gene            2521..3429
                     /locus_tag="PLI16_06200"
     CDS             2521..3429
                     /locus_tag="PLI16_06200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOH84189.1"
                     /translation="MISLILIVFCTVLFPGIIVRTKSIASGRKGPGVWQPMKDIVRLF
                     RKGAVYSNTSSVIFRVAPLVYFASLLMAMMLLPFGKHPGILSFEGDFIFFAYVMTAGK
                     FLMIIGALDTGSSFEGMGASREALYSMLIEPAFFVLMAAFAMFTGHTTFYDIFQTLYL
                     DSYLAIFAVILAVYILFQIAMIENSRMPYDDPKTHLELTMVHEVMVLDNSGFDLGLIQ
                     LASALKFAVFGSLMANLFIMPWMPWYLAVLIFLAVQFSFAVSVGIVESFRARHRLRIN
                     NQAILTITPIAVLVFFSILLIITKQF"
     gene            3432..4061
                     /locus_tag="PLI16_06205"
     CDS             3432..4061
                     /locus_tag="PLI16_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84190.1"
                     /translation="MATLLIIIFIITLFYAAISVRMFTLINLLMVQGFLLFGVAFIEL
                     RELNVVNLIIILLETLVFKAFLVPYFLKRTIRQNNIKREVEPFISGVTSLLIVSVLIV
                     LSFMLTFSLQQDFYNVIYFTVAFSAILTGLFLMVSRKKIITHVIGYVILENGIVLLAF
                     SVGNNMPMIVNAGILLDILVSVLLFGFFMSRIRYISKEFEISSLSKLKD"
     gene            4061..>4585
                     /locus_tag="PLI16_06210"
     CDS             4061..>4585
                     /locus_tag="PLI16_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84191.1"
                     /translation="MALAYFITVIVLALILAFAKNRLAQLLVMTVFSLVQTAFTAYVI
                     FFREVGFSGYFAVDSLCLIFLSLLTVVTITTVIQSFVYLRKRNDTLAHRSYYMASLVF
                     FVASMTGVFLSDHIGLMWVFAEATSLSIALLIYHERTPEAIEATWKYIFISTLGLSFS
                     FINILFQQQRFVSGN"
BASE COUNT         1047 a          996 c         1019 g         1523 t
ORIGIN      
        1 ttggtgataa aaaatgcgat aaccgcaatg atgatagcgc cgatgcctcc aatacctgca
       61 atgcctttgc cgctgatgct acggcggtct tctacattat cactttgtct tctgcctgac
      121 catttcatag ttttattgtt tagttttttc caaatttaat aatattttta aaaataataa
      181 ataacagatg gtatattttg aaatattctt ttgaaaatgt gataaaaaac aacatagaag
      241 aaccacggcc ttgaacttct cttttctctg gtagctcttt gtaattaaaa tactcatcat
      301 acccacgttg gcaacgcata caacaatttt aatttcgtaa ataacctttt accaaagctt
      361 ctatgtgtca actaaataaa actaatacat gctgttggct catttaccgc tgagaagttc
      421 ttgctgatag cccctgccat taaggctatt cctgataatt aaccatgaac ggttgattat
      481 tttcctattt gtatgggtct ataaaaaata ttccttatac atttgcccca aatttcaggt
      541 gatgattttt ttgttactga cggtatgttc tttcttgatt ttcgttgtcc cgcgcaaagc
      601 aaaagtgtat agtaacttgt tgcttaacac ggtcttggct gcttatacct tattgctgtg
      661 cggtggggta tttatgggtg cgggtactta cacagaagta ttacctgtat atttctgggg
      721 acagcagata aaattgatta ttgacccttt gtcggctttt tttattggca taatcaactt
      781 cacggtgctg acagcttctg tatatgccat cggatacatg aagatgtacc gcaacaaatc
      841 ttcagtagaa ctttctatac attatctttc attttttcta ttacatctgt cgatgattct
      901 tgtctgcatg attcatcacg ggattgcctt tctggtggta tgggagctga tggctatttc
      961 ttcttttctg ctggtgattt ttgataatga aaaaagcagt gtgctgaagg ccggtatcaa
     1021 ctacctgata caaatgcatg ttggcgcctt attcttaatt gtggccttca ttattctcaa
     1081 tatcaatacc ggttcatttg attttaaagc gttgaaatct tttttctata accaccagaa
     1141 cctgccgttg tttcttttgt tttttgtcgg ctttggcatt aaggctggct ttatgcccct
     1201 gcatacctgg ctgccgcatg ctcaccccgc ggctcccacg catgtttccg ccgtgatgtc
     1261 gggcgtgatg atcaaactgg gaatctacgg aatactaagg gtcgccatgt ttttgcaggc
     1321 tgatattttg tggataggca tcatagtgct gctcatctcc ataatttccg gcatcggtgg
     1381 cgtgtcgtgg gctatcgtac agcacgacct gaaaaaactt ttggcctatc acagcatcga
     1441 aaatattggc atcataggta tcggaatcgg tattggcctc atcggcctgg gcgctgaaaa
     1501 ctccctgctg gctatcctgg gattcgcggg cgccatgctt cacgtcctca accattccct
     1561 gttcaagtcg ttgttgtttt atggtgccgg agccgtttac ctcaaaacgc acacccggaa
     1621 tatcgaactg cttggcgggc tgataaaaaa aatccctgtt acggctattc tttttctgat
     1681 agcgtcgctg gctatctgcg ggttgccacc ctttaatggc tttgtttcgg agtttattat
     1741 ttataaaggc atcattgtcg ggcttaacga accgtctatt ggcgttaaag tgctgatgct
     1801 tgcggccatt atcagcctgg tgctcattgg cgggttggcg gtgttttgtt ttaccaaggc
     1861 tttcggtgtg acatttctgg gaaaatcgcg ccatgtggat atctctggtg taaatgagtt
     1921 gtcagggcta atgctggcgc ctttgtttct tattgctgcc gccatactct ttataggtct
     1981 gtttccgcaa tgggccattc agccggttaa ttttgtgctt gaaagccgcg cttttaacat
     2041 tgccggcaat agcgatatga tgtatgccct gcctatgatt caaaaaatag gggtagcggc
     2101 agcgttgttt attggcctga ccgggctggt gtggctatgg cggtttgtca tgaccaggaa
     2161 aaagccgctt acccattccg ggacctgggg ctgcgcttat gatggcgcca ttgaaaaagg
     2221 gcaatacaca gccacctctt atgccgaaaa ctttaccgat atagccggac ctctggtgaa
     2281 tgtaaaaaca cagtatcacg ccttgcatga ggatgacatt ttccccgaaa gccgcagttt
     2341 tgagactttc agcgaagata tgatggagaa aaagctatac gggcaatccg ttggctggtt
     2401 gaacagggca ttatcacgcc tggctgtcat acaaaccgga aacacccagc attatatttt
     2461 gtatgccttt gctatgattc ttctcttgct cctgctgacc ttgtttaaca tattataatc
     2521 atgataagcc ttattctgat agtgttctgt accgttcttt ttcctgggat tatcgtccgg
     2581 acaaaaagta ttgccagcgg cagaaaagga ccgggggtat ggcaacccat gaaggatatc
     2641 gtgcggttgt tcagaaaagg tgcggtatac agcaatacca gcagtgtgat tttcagggtg
     2701 gcgccgttgg tgtattttgc ctcgctgctt atggcgatga tgctgttgcc ttttggaaaa
     2761 catcccggca tattgtcttt tgaaggtgat tttatctttt ttgcctatgt catgactgcc
     2821 ggaaagttcc tgatgatcat aggagccctg gatacgggca gcagttttga gggcatgggt
     2881 gccagccgcg aagccttata ttccatgctt atcgaaccgg ctttttttgt gctgatggcc
     2941 gctttcgcca tgtttaccgg gcataccacg ttttacgata tttttcagac cttatacctt
     3001 gattcatacc tggctatttt tgccgtcatc ctggcggtat atatactgtt tcagatcgcc
     3061 atgatagaaa acagccgcat gccttacgac gatcctaaga cacacctgga actcaccatg
     3121 gtacacgagg taatggtgct cgacaacagc ggtttcgacc tgggactgat acagttggcc
     3181 tctgccctga agtttgccgt ctttggcagc ctgatggcta acctgttcat catgccgtgg
     3241 atgccctggt atcttgccgt gctgatcttt ttagccgtgc agttttcttt tgccgtgtcg
     3301 gtaggcattg tcgaatcctt cagggcccgt caccggctgc gtattaacaa ccaggcaata
     3361 ctcaccatca cgcccatcgc cgtacttgtc tttttcagca tattattaat aattaccaaa
     3421 cagttttaaa gatggccacc ctgctgataa taattttcat catcactttg ttttatgcgg
     3481 ctatttcggt gcggatgttt acccttatta acctcttgat ggtacaggga ttcctgcttt
     3541 ttggcgtggc cttcattgag ctgcgcgagc tgaatgtggt caacctgatc attatcctgc
     3601 tagaaactct ggtatttaaa gccttcctgg taccttattt cttaaaaaga accatcaggc
     3661 agaataacat caaacgcgag gtagaacctt ttatttccgg agttacatcc ctgctcattg
     3721 tttcggtgct gatagtactg agttttatgc tcactttttc tttgcagcag gatttttaca
     3781 atgtcattta ttttacggta gctttttcgg ccatacttac gggcctgttt ctgatggtca
     3841 gccgtaaaaa aatcatcaca cacgtgattg gttatgtcat tttggaaaac ggcattgtgc
     3901 tgctggcttt ttcggttggc aacaatatgc cgatgatagt caatgcggga atactgcttg
     3961 atatcctggt cagtgtgttg ttgtttggct tttttatgag ccggatacgg tatatttcca
     4021 aagaatttga aatttcttca ttaagtaaac tgaaagactg atggcgctgg catattttat
     4081 aacggttatt gtcctcgcgt tgatactggc ttttgcgaaa aacaggttgg cgcaattgct
     4141 ggtcatgacg gtgttttcgt tggtgcaaac ggcctttacc gcgtatgtta tctttttcag
     4201 agaggtcggt tttagtggct attttgcggt ggactccctc tgtctgattt ttctctcatt
     4261 actgactgtg gttactataa ccaccgttat tcagagtttt gtgtatctgc ggaaacgtaa
     4321 cgataccctg gcccataggt cttattatat ggcttcgctg gtgttttttg ttgccagcat
     4381 gaccggggtg tttctttcgg atcatatcgg cctgatgtgg gtttttgccg aagccacctc
     4441 actgtctatt gctttgctca tttaccacga acgcacacct gaggcgatcg aagcaacctg
     4501 gaaatacatt tttatttcca ccctcggact ttctttttct tttatcaata ttttatttca
     4561 acaacaaagg tttgtgtccg gcaac
//
LOCUS       DAOSFP010000178         4578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_16917_length_4578_cov_4.005969, whole genome shotgun sequence.
ACCESSION   DAOSFP010000178 DAOSFP010000000
VERSION     DAOSFP010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16917_length_4578_cov_4.005969"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2133)
                     /locus_tag="PLI16_06215"
     CDS             complement(<1..2133)
                     /locus_tag="PLI16_06215"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOH84192.1"
                     /translation="MKKISIFFIGLICTAVLFVFSHQAARAQLTVVEGAALNMTPLQL
                     VQQVLVGSGVTVSNATFNGSAASISSNAIGSFTAASVAGTQLGFGGGILLTSGQASLA
                     IGPNTNSGAGFNTNLTADPDLQLLVSTTVYDKAVLEFDFVPISDTIRFRYVFGSEEFD
                     EFCASSYNDVFGFFISGPGITGPFTGNAKNIAVMPNNPANYVTINNICTAGATYSWLN
                     SGGTYFQYDRMTYVYTAWSLVTSCQTYHIKLAVGDAGDHILDSGVFLEANSFSSNGVS
                     YNTSFSSQVDTVAVEGCNDAIVTFHLGQPDVIPTVINYTVSGTAIEGVDYPNVPDSLI
                     IPAGQDSAYFVIHPIADGLAEPGETVIISYVNTVCGTLDTIMILINDYTPLYTISITP
                     EVKNCGGQAANINITAGGGYTTAAHGLSYEWSNGAGNTAAVVVNPATPTMYYVTVSDA
                     CFFFNDSASYYIVDSVMVSISNLQNTITNVDSVTCFGYSDGSATVTATSGLEPYHYLW
                     SPTNDTTSTASNLAAGTYFVTVTDNIGCTSGNFVILNDPPEIGLLLSPTDETCYTFCN
                     GSIATQVTGNYSQPVNWAWSTTPAQNTPNANNLCPGTYTVTFTYSIHDCFVVESADII
                     TGTPITADFTATPDSGFVPLTVQFTYTGIGATVFYWDFGDGTSSTAANPQHTYTDKGL
                     YTATLSVSSGPPNFCEETKQVEIEVIHPS"
     gene            2277..3254
                     /gene="dusB"
                     /locus_tag="PLI16_06220"
     CDS             2277..3254
                     /gene="dusB"
                     /locus_tag="PLI16_06220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOH84193.1"
                     /translation="MNEFVRKIFSDFVLALAPMEDVTDTAFRRICKSYGADLVVTEFV
                     AAEGLLRCAEKSKKKLIFDECERPIGIQLFGNNKDSIVAAAKIAEEANPDFIDLNFGC
                     PVKKIVDKGGGAALLKDIPKMLDITSAVVNHARKPVFVKTRLGWDEKNIVMPQLAEQL
                     QDAGISALTIHGRTKSQMYKGKADWSLIGEVKKNPRIVIPIIGNGDVDNPQFALEMKN
                     RHGVDGVMIGRAAIGNPWIFKDIKEYIKFGKLPTPPLIEERIEMVTGHLLSSVNNKGE
                     RTAILEMRKHYSTIFRALENFKQFRTKLVSTNTLDETLAVLKEILEFYK"
     gene            3284..3823
                     /locus_tag="PLI16_06225"
     CDS             3284..3823
                     /locus_tag="PLI16_06225"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Reeler domain-containing protein"
                     /protein_id="HOH84194.1"
                     /translation="MRTKTIFLIVITTLFAVGFFSLQLFSYPGGAPAAKTGSPADAKT
                     CMACHKATLKKQEGMITSDAENNMYEPGKTYTIQAAAKGSANTVRIGFEVSPQNAAGE
                     LLGTLILTNPDETKLIGKGKYITHTDQGSRAQGSKKWSFNWKAPAAGTGDVTFYGSFM
                     VSESSQLVYTSTLTLKEKK"
     gene            complement(3832..4467)
                     /locus_tag="PLI16_06230"
     CDS             complement(3832..4467)
                     /locus_tag="PLI16_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOH84195.1"
                     /translation="MSAQKIKNIIFDFGGVIIDIDYWRSINAFISLGAKDFDGIYSQA
                     GQIEVFDKLDKGLINPDDFRKELTRFVPPETTLQMIDDAWNAILLGIPEHRIRLLEKI
                     RKNYRLILMSNTNIIHYDIYSKELKEKFGYDTLGCLFDKVYLSFELGMRKPDTAFFKL
                     ILDENHLDPATTLFIDDSEQNLPPAQNLGIQTIFLKKGRDVSDLFADGFLK"
BASE COUNT         1261 a          963 c         1154 g         1200 t
ORIGIN      
        1 ggaggggtgt atgacttcaa tttccacctg tttggtttcc tcacagaaat tgggcggccc
       61 gctgcttacc gacagcgtgg cagtatataa tcctttatcg gtataagtgt gttgtggatt
      121 ggcagccgta ctgctagttc catcaccaaa gtcccagtaa aaaacagtgg ctccgatgcc
      181 ggtataggta aactgcactg tcagcgggac aaaaccggag tcgggagtag cggtaaaatc
      241 tgctgttatc ggggtcccgg taattatgtc ggcactttcc accacaaaac agtcatggat
      301 tgaataggta aatgtcacgg tataagtccc ggggcacaaa ttgttggcat tgggggtgtt
      361 ttgcgccggt gtggtgctcc atgcccagtt taccggttgc gaatagttcc ctgtcacctg
      421 tgtggctatg gacccgttac aaaaagtgta gcaggtttcg tccgtagggc tcaacagcag
      481 ccctatttca ggcggatcgt tcaggatgac aaagttgccg gaggtacaac cgatattgtc
      541 tgtaaccgtt acaaaataag tgcctgctgc aagattgctg gcggtgctgg tagtatcgtt
      601 ggtgggcgac cacaaataat gatagggttc caggccgctt gttgcggtaa cggttgcaga
      661 accgtcgctg tatccgaaac aggttacgga atccacattg gtgatggtat tttgcaggtt
      721 gctgatactt accataacgc tgtcaacaat atagtaactg gcagagtcat tgaaaaagaa
      781 acaggcatca gacaccgtta cataatacat ggttggagtg gcaggattaa caactactgc
      841 tgccgtgttt cctgcgccgt tgctccactc atagctgagt ccgtgggcgg cagttgtgta
      901 accgccgccg gctgtgatgt tgatgttggc tgcctggccg ccgcaatttt ttacttcggg
      961 tgtgatagaa atggtgtaca gtggagtgta atcgtttatc agaatcataa tggtgtcaag
     1021 ggttccgcaa acagtgttca cataagaaat aatcactgtt tcaccgggtt cggcgaggcc
     1081 atcggcgata ggatgaatga caaagtacgc ggaatcctgg ccagcaggga tgataagact
     1141 gtcgggaacg ttgggatagt caacgccttc gatggcggta ccgctcacgg tatagttaat
     1201 cactgtcggg ataacgtcag gttggccaag atgaaaggta acgatggcat cgttacatcc
     1261 ttcgacggct accgtatcga cctgtgaaga gaaagaggtg ttgtaggaaa ccccgttgga
     1321 tgaaaaactg ttggcttcaa gaaaaactcc cgaatccaga atgtggtcgc cggcatcgcc
     1381 tacggccagc ttgatatggt atgtctggca ggaagtaaca aggctccatg cagtgtacac
     1441 atatgtcatc ctgtcgtact gaaaatacgt gccgcctgaa ttcagccagg agtaagtggc
     1501 gccggcagta caaatgttgt taatggtaac atagttggcg gggttgttag gcataacggc
     1561 tatgtttttg gcattgccgg taaaaggtcc ggtgatgcca gggccgctga taaaaaagcc
     1621 gaaaacgtcg ttataactgg aagcacaaaa ttcgtcaaac tcttcggatc caaacacata
     1681 ccggaagcgg atggtgtcgg aaattggcac aaagtcgaat tccagcacag ctttgtcgta
     1741 cacggtggtg gacactaaca gttgcaggtc gggatcggca gtcaaattgg tgttgaatcc
     1801 tgcacccgaa ttggtgtttg gcccgatggc aagcgaggcc tggccggaag taagcaaaat
     1861 gcctcctcca aaaccaagct gtgtcccggc aacagaggcc gcggtaaatg acccgatggc
     1921 attggaagaa attgatgctg ccgaaccgtt aaaagtagca ttacttaccg ttacgccact
     1981 gcctaccaat acttgttgta ctaattgcag tggtgtcata ttgagtgcag cgccttcaac
     2041 aacggttaac tgcgcccggg ctgcctgatg cgaaaaaaca aaaagtactg ctgtacagat
     2101 tagtccaata aagaatattg atattttttt cattatttga tttttttgtt cttgctttgg
     2161 gttacgaata atacctattt agacatcaaa ataatatatt tggttgcttg attacgctaa
     2221 attaatactt ttgtttcaaa aaaatgttaa tagcagtatt ttaataaaaa attatagtga
     2281 acgaatttgt ccgtaaaata ttttccgatt ttgtacttgc actggctcca atggaagatg
     2341 taaccgatac ggcctttcgc cgtatatgca aatcctatgg cgccgacctg gtggttaccg
     2401 agtttgtggc tgccgaaggg ctgttgcgtt gtgcagaaaa gagtaaaaaa aaactaattt
     2461 ttgacgagtg cgagcggcca atagggatac agcttttcgg gaataataaa gattctattg
     2521 tggccgcagc caaaatagcc gaagaggcta atcccgattt tattgatttg aatttcggat
     2581 gtccggtaaa aaaaatcgtg gacaaaggcg gtggggccgc gctgttgaaa gatatcccaa
     2641 agatgctcga cataacaagc gccgtggtca accacgccag aaagcccgtg ttcgttaaaa
     2701 cacgcctggg ctgggatgaa aaaaatatcg tcatgcctca actggcagaa cagcttcagg
     2761 atgccggcat cagcgccctt actatccatg ggcggacaaa atcacagatg tataaaggca
     2821 aagccgactg gtcgctcatt ggcgaagtga aaaagaatcc gcgtatagtc atccccatta
     2881 ttggcaatgg cgatgtggat aacccgcagt tcgcactgga aatgaaaaac cgtcacggag
     2941 ttgatggtgt aatgattggc agggcagcta tcggcaaccc gtggattttt aaagatatca
     3001 aagaatatat taagtttggg aaactcccca caccgccgct gattgaagaa aggatagaaa
     3061 tggttaccgg ccatctgctc agctctgtaa acaacaaagg agagcgcacc gccatactcg
     3121 aaatgcggaa acattacagc accattttca gggcgctgga aaattttaag cagtttcgta
     3181 ccaaactggt tagtaccaac accctcgatg agactctggc cgtattaaaa gaaatcctgg
     3241 aattctacaa ataattttat tatctttgaa ataaaaaatt gccatgagga ctaaaaccat
     3301 ttttttgata gtaattacta ctttgtttgc tgtgggcttt ttcagtttgc agctattttc
     3361 ttatcccgga ggcgctcctg cagcaaagac cggctcaccg gccgatgcta aaacatgcat
     3421 ggcttgtcat aaagccacct tgaaaaagca ggaaggtatg attacttccg atgctgaaaa
     3481 caatatgtat gaacccggta aaacctatac catacaggca gcggccaaag gttcggccaa
     3541 taccgtgcgc attggctttg aggtgtcgcc gcaaaatgcg gccggagaat tgctgggaac
     3601 gcttatcctt acaaatcccg atgaaaccaa gctgattggc aagggaaaat acattactca
     3661 taccgaccag ggctccaggg cacagggaag caaaaagtgg tcgttcaatt ggaaagctcc
     3721 tgcggccggt acaggcgatg ttacgtttta cggttcgttt atggtatccg agtcttcgca
     3781 gttggtttat acttccaccc tgacgctgaa agaaaaaaaa tagtccggta attatttcaa
     3841 aaagccgtct gcaaaaagat cgcttacatc cctgcctttt ttgaggaaga tggtttgtat
     3901 gcctaagttt tgtgccggag gcaggttttg ttccgaatcg tcaataaaca aagtggtggc
     3961 tggatcgagg tggttttcgt ccagtattag cttgaaaaaa gcggtgtcgg gtttgcgcat
     4021 accaagttcg aaggatagat acactttatc aaacaggcag cccagcgtgt cgtagccgaa
     4081 tttttctttc agttctttgc tgtaaatgtc gtaatggatg atattggtat tgctcatcag
     4141 aatcaagcga tagttttttc tgattttttc cagcaggcgt atgcggtgtt cgggtatccc
     4201 cagcaaaatg gcgttccagg catcatcgat catctgcagg gtggtttcgg gcggtacgaa
     4261 tcgggtaagt tcttttcgaa agtcatcggg gtttatcagt cccttatcaa gtttgtcgaa
     4321 gacctcaatc tgtccggcct gggaatatat gccgtcaaaa tctttggctc ccagactgat
     4381 aaatgcgttg atgcttctcc aatagtcgat atcgatgatg actccaccga aatcaaaaat
     4441 gatgttttta attttttgtg cggacataaa aaaattattt ggaaaactgg gtgcaaagat
     4501 gtaattttgc tgccacaaaa ccaaatacgt tgcctgataa gcaataggtc ctatagctca
     4561 gttggttaga gcacctga
//
LOCUS       DAOSFP010000179         4539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17119_length_4539_cov_4.021855, whole genome shotgun sequence.
ACCESSION   DAOSFP010000179 DAOSFP010000000
VERSION     DAOSFP010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17119_length_4539_cov_4.021855"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLI16_06235"
     CDS             complement(<1..926)
                     /locus_tag="PLI16_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84196.1"
                     /translation="MTILKKYKLFVFLLLALAISNFSTAQIRISSPYSRYGLGDLQTS
                     HSTYAAAMGGLSAALRHPNYINVNNPASYTSFDTNSFVLDVGIASTFNQVKTNMVTQG
                     FTNHTSIGHVLFGFPATRWCGISFGILPYSKSGYQVKTSDTIINSDETFTESFKGSGG
                     VNQAYIGVAFKPFKNFSFGANLGYLFGTIDKIVAIERPDDIFSFNTRVTDNEQVGGLY
                     LNYGLQYQILLKKKHMLSLGTKFNLPMNVGARQTMLAERYSGLLEEDNISIKDTLLNV
                     DGEKGKLHMPLIISGGFTLLINNTWLAGADFE"
     gene            complement(904..1635)
                     /locus_tag="PLI16_06240"
     CDS             complement(904..1635)
                     /locus_tag="PLI16_06240"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOH84197.1"
                     /translation="MKLAIDLGNSYQKAAVYSEDSLIYVEKTENLSDSIGKIFSSYPI
                     DGAIISSVITNYYKLTDFIKTKCPLLFFDEHTPIPITNAYGTPETLGKDRLASVVAAN
                     HLFPGQNILVIDAGSCIKYDYIDALATYHGGAISPGLTMRFKALHTFTGKLPLIELTE
                     FCQLTGYNTQQSILSGVINGTVAEINGIISQYNSSYPELKVVLSGGDSEYLVNKLKSK
                     IFAVPNIVLFGLKIILDYNDNIKKI"
     gene            1750..1822
                     /locus_tag="PLI16_06245"
     tRNA            1750..1822
                     /locus_tag="PLI16_06245"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1783..1785,aa:Phe,seq:gaa)
     gene            1843..3195
                     /gene="tilS"
                     /locus_tag="PLI16_06250"
     CDS             1843..3195
                     /gene="tilS"
                     /locus_tag="PLI16_06250"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOH84198.1"
                     /translation="MLFLFIFVSMLEGFLDFIEKNKLCGKSDHLLLAVSGGVDSVVMA
                     ELFHRAARHFSIIHCNFQLRGTASDTDEQFVRALAQKYYTTCFVKSFDTRKYAALHKL
                     SIQQAARDLRYAYFEEIRKKENFDLIATAHHADDQIETFFINLFRGSGITGLAGIPIK
                     QQHIIRPLLFAFRAEIEAFAGDNNLEYRTDASNNENKYLRNKIRNLLMPQLENLSPSC
                     RTTINTAIGHLKNTDQIYHQHLKNIRLLKKHKNGLITIDIPKLKKLQPTSHYLFEYIS
                     VYGFNSRVCDEICQSLDEISGKIFFSPSHRLVKDRDTLIIDKKPDSAACEEFVLEEGV
                     KKIVTPVNLHFLEKSIVVLKTFTVPGNTALIDKDKLTYPLIIRRWRQGDYFYPLGMNC
                     KKKLSDFFTDNKLSVIDKSNIWLLCSGEKIVWIIGYRTDNRFKITERTQKVVQVKLKK
                     "
     gene            3237..>4539
                     /locus_tag="PLI16_06255"
     CDS             3237..>4539
                     /locus_tag="PLI16_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOH84199.1"
                     /translation="MILHKRLVLFVLLVLSFSVAEAQILEPVKWSFASKKTSDTEAEL
                     IFTATIDNGWHLYSQDIPPNGPVATEFVFDKSTAFSRVGKVVEPKPIVEKDPNFDDML
                     LKFFSTKAVFKQKIKLLSNKPGVVKGMVSFMCCNDVSCLPPNDVPFNIKIEGATAEVT
                     DTVITETADTLTEKDTAAITPNDTINKGTLSADGTKDVGNMGLLNLFIFAFLAGLAAI
                     ITPCVFPMIPMTVSFFMHEGESRAKSIAKAIVFGLSIIFIYTVIGTIVAVTLGVNFAN
                     FLSTHWIPNVLFFLIFLVFAASFFGMFEITMPSWMINKTDKQVDKGGILGSFFMAFTL
                     VLVSFSCTGPLVGAILVKSASGQVLEPIIGMLGFSVAFAFPFTLFAFFPSWLNRLPKS
                     GGWLNTVKVSLGFLELALAFKFLSNADLVLQLHLFERETFLA"
BASE COUNT         1293 a          933 c          982 g         1331 t
ORIGIN      
        1 cattcaaagt ctgcgccggc cagccaggtg ttgtttatca gcagggtaaa tccgccgctg
       61 ataatcaatg gcatgtgtaa tttacctttt tcgccatcaa catttaataa agtgtctttt
      121 atgctgatat tatcttcttc cagcagtcct gaatatcttt cggcaagcat ggtttgcctg
      181 gcaccgacat tcatgggaag gttgaatttg gtgcccagcg acagcatgtg ttttttcttc
      241 agcagaattt gatattgcaa tccatagttg agatacaagc cgcccacctg ctcgttgtcg
      301 gtaaccctgg tgttgaaaga aaaaatgtcg tcggggcgtt caatagccac tattttgtcg
      361 atagtgccaa aaaggtatcc caggttggct ccgaatgaga aatttttaaa aggtttgaaa
      421 gcaacaccaa tgtatgcctg gttgactccg ccactgcctt tgaagctttc agtaaatgtc
      481 tcatcagaat tgataatggt atctgaagtt tttacctgat atcccgattt ggaataggga
      541 agtattccaa aagaaattcc gcaccagcgt gtagccggaa aaccgaataa tacatgccct
      601 atggaggtgt gattggtaaa accctgcgta accatattgg tctttacctg gttaaaagtg
      661 ctggcaatgc ccacatccaa cacaaaagag ttggtgtcga acgaagtata agaagcggga
      721 ttgtttacat taatataatt ggggtggcgg agtgcagcgc tcaatccgcc cattgccgca
      781 gcataggttg agtgacttgt ctgcaaatca ccaaggccat aacgggaata aggcgaactg
      841 attcgtatct gcgccgtgct gaaatttgaa atagccaagg ctaataacaa aaagacgaat
      901 aatttatatt tttttaatat tgtcattata gtcgagaatt atttttaagc cgaacaaaac
      961 tatatttgga accgcaaata tcttactttt tagtttattg accaaatatt ctgagtctcc
     1021 gcccgataat accactttca gttccggata gctgctgtta tattgcgaaa tgattccgtt
     1081 aatttcagca acagtgccat tgataactcc cgagagtatt gattgctgcg tgttgtaacc
     1141 ggtaagttga cagaattcgg tcagttctat taacggaagt ttccccgtaa aagtatgcaa
     1201 ggccttaaaa cgcatggtga ggccgggcga aatagcaccg ccgtggtatg ttgccagggc
     1261 gtcaatataa tcatacttta tgcaactgcc tgcatcaata accaatatat tttgtccggg
     1321 aaacaaatgg ttggcggcca ctacagaggc cagacggtct ttccccaggg tttccggcgt
     1381 gccataagcg ttggttatgg gtataggcgt gtgctcatca aaaaataaca aagggcattt
     1441 tgttttaata aaatccgtaa gcttatagta attggttatc accgaagaaa ttatggcgcc
     1501 atctatcgga taagaagaaa agattttccc tatgctgtcc gataaatttt cagttttttc
     1561 aacatagatt aaagagtctt ccgaataaac ggcggctttt tgataggagt tccccaggtc
     1621 gatggccagc ttcattatta gtaatttaca atttacaaaa ttacgttatt cgaagaaatt
     1681 tatcattttt tttgattaga tttttgtaga actaaaaaaa attgtatttt tgcagtcctt
     1741 tttatgggag gcgacgtagc tcagttggta gagcagagga ctgaaaatcc ttgtgtcact
     1801 ggttcaattc cagtcgttgc cacagaaagc acatcgcagg atgtgctttt tttgtttatt
     1861 tttgtttcta tgcttgaagg atttcttgat tttattgaaa aaaataaact gtgcggcaag
     1921 tctgaccatc tcctgctggc cgtaagcgga ggtgttgatt cggtagttat ggcggaactt
     1981 tttcaccggg cagccaggca tttcagcatc atacactgca attttcaatt gcggggcacg
     2041 gcctccgata ccgatgaaca atttgtgaga gcgctggcac aaaaatacta taccacttgt
     2101 tttgtgaaaa gttttgatac gagaaagtat gcggccctgc ataaactttc tattcaacaa
     2161 gccgcccgcg acttgcggta tgcctatttt gaagaaatca gaaaaaagga aaattttgat
     2221 ttgattgcca cagctcatca tgccgacgac cagatagaaa ctttttttat taacctgttc
     2281 agaggcagcg gtatcaccgg actggcaggc attcccatca aacagcaaca tattatccgt
     2341 cccctgttgt ttgcattcag ggccgaaata gaagcttttg ccggtgataa taatctggaa
     2401 taccgcaccg atgcgtcaaa caacgaaaat aaatacctgc gaaacaagat cagaaacctg
     2461 ctgatgcctc aactggagaa tttgtcaccc tcatgccgca ccacgatcaa cacggccatt
     2521 ggccacctaa aaaatacaga ccaaatttac catcagcatc ttaaaaatat caggctgcta
     2581 aaaaaacaca aaaacgggct gattactatc gacatcccca agcttaaaaa actacagccg
     2641 acatcgcatt acctgtttga gtatatttcg gtttatggct tcaacagccg tgtatgcgat
     2701 gaaatctgcc agtcgctgga cgaaatttca ggaaaaatat ttttttcgcc ctcgcaccgc
     2761 ctggttaagg acagggatac ccttatcatt gataaaaagc cggattccgc tgcctgcgaa
     2821 gaatttgtgc tggaagaggg agtgaaaaaa attgttacac ctgtaaacct tcattttctt
     2881 gaaaaaagca ttgtggtatt aaaaactttt actgtccctg gaaataccgc tctgattgac
     2941 aaagacaaac tgacctaccc cctgataata cgccggtgga ggcaggggga ttatttttat
     3001 cccctgggga tgaactgcaa aaaaaaacta agtgattttt ttactgataa caaactgtct
     3061 gtcattgata aatcaaatat ctggctgcta tgctccgggg aaaaaattgt atggattatt
     3121 ggatacagaa ccgacaaccg gtttaaaata accgaacgga ctcaaaaggt ggttcaggta
     3181 aaattgaaaa aataaatgat taaatttgta ccttaatatt ttcaagaaaa atttatatga
     3241 ttttacataa aaggttagta ttatttgttc tgttagtttt aagcttttct gttgccgaag
     3301 cccagatact ggaacccgtg aaatggagct ttgcatcaaa aaaaacttcc gacactgaag
     3361 cagaactgat ctttacggcc actattgata acggttggca tttatattcg caggatattc
     3421 cgcccaatgg tccggtagct accgaatttg tatttgataa aagtactgct ttcagccggg
     3481 ttggtaaggt tgtggagcca aagccgattg tggaaaaaga tccgaatttc gacgacatgc
     3541 tgctgaagtt tttttcgacc aaagcagtat ttaagcaaaa aataaaactc ctgagcaaca
     3601 agcccggagt ggtaaaaggc atggtgagtt ttatgtgttg caacgatgtt agctgcctgc
     3661 cccccaatga tgttcctttt aatataaaaa tagaaggcgc tacggctgag gttaccgata
     3721 cggtgattac cgaaacagcc gataccctaa ctgaaaaaga cactgctgcc attactccca
     3781 atgatacaat taacaaaggc accttgtcgg ctgacggcac caaagacgtg ggcaatatgg
     3841 gcctgctcaa cttgtttatt tttgcttttc tggccggact ggcagccatc atcactccct
     3901 gtgtattccc tatgatcccg atgacggttt cattttttat gcatgaaggc gaaagccgcg
     3961 ccaaatcaat agcaaaggcc attgtctttg ggctgtctat catttttatc tatactgtca
     4021 taggaactat cgtggcggta acgctggggg ttaacttcgc caattttctc agcactcact
     4081 ggatccccaa tgtgttgttt ttcctgatat ttttggtttt tgccgcttcg tttttcggaa
     4141 tgtttgaaat taccatgccg agctggatga tcaacaaaac cgacaaacag gttgataaag
     4201 gcggcatcct gggatctttt ttcatggcct tcaccctggt tctggtgtcg ttttcgtgta
     4261 cgggaccgct ggtaggggcc atcctggtga aatcggccag cgggcaggtg ctcgagccca
     4321 tcatcgggat gctgggtttt tcggtggcct tcgcgtttcc gtttacctta tttgcttttt
     4381 tcccatcgtg gcttaaccgc ttgccaaaat caggcggatg gttaaatacc gtgaaagtgt
     4441 cgttaggatt tttagaattg gctttggcgt ttaaattctt atccaatgcc gatttagtat
     4501 tacaattaca tttgtttgaa cgcgaaacat ttttagcga
//
LOCUS       DAOSFP010000180         4510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17307_length_4510_cov_4.238608, whole genome shotgun sequence.
ACCESSION   DAOSFP010000180 DAOSFP010000000
VERSION     DAOSFP010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17307_length_4510_cov_4.238608"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..442
                     /locus_tag="PLI16_06260"
     CDS             104..442
                     /locus_tag="PLI16_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOH84200.1"
                     /translation="MPYKQTPTEKIYYTIGEVAKMFDVNTSLIRFWEKEFDIIKPFRN
                     KKGNRLFTKADVDNFYIIYHLVKERGFTLQGAKDKMKENKVDTINNVEVVASLNKIRD
                     FLLELKEELD"
     gene            complement(547..3306)
                     /gene="ppdK"
                     /locus_tag="PLI16_06265"
     CDS             complement(547..3306)
                     /gene="ppdK"
                     /locus_tag="PLI16_06265"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOH84201.1"
                     /translation="MATKSKKYVYYFGGKKAEGRADMKNLLGGKGANLAEMVNLGLPV
                     PAGFSITTECCTDYYANKCQLPKSLTAEVWAGMKKVEAEMGMKYGDKNNPLLLSCRSG
                     ARASMPGMMDTVLNIGLCTATIPGLIKKTNNPRFVWDSYRRLIMMYADVVMDKAEGLD
                     KDIRKQLDDMLNEYKKSKKVKQDTELTADDLKVLAGEFKKKVKKEFGKEFPDNAEEQL
                     FGGIRAVFKSWNGKKAVSYRRIEGIPDDWGTAVNVQAMVFGNMGDTSATGVAFTRNPA
                     TGENLFYGEWLINAQGEDVVAGIRTPNPLNDDTKNEQNKHLHSMQEQMPKTYKELDKI
                     RNILEKNFRDMLDIEFTIQETKLYMLQCRVGKRTGTAALKMAMDMLKEKLIDEKTAVL
                     RVDPAQLDELLHPICDPAEEKKVDTLVKGLPAGPGAAVGQLVFTAEDAVAWERKGKKV
                     ILIREETNPEDVEGMRSAVGILTARGGMTSHAALVARGWGKCCIVGAGKLHVDVKTKT
                     AKVEGTNIVLKEGDVVTLNGTKGHVYQKSLKLMDASENPLFKHFMKMVDSFRKMGVRT
                     NADNPADAKTARDFGAEGIGLFRTEHMFYGAGSEKPLLALRKMILSNSREERVKALNQ
                     LYPFVKKDIKATLLAMDKLPVTFRLLDPPLHEFVPQTPDKQAELAKELNISVADIAKR
                     GEGLHESNPMMGHRGVRLSVTYPEVSEMQFRAIFESAQELINEGYHPHPEIMVPVTCE
                     VGELDVTKKIADRVYDEVCKKYKVKEIKYLYGTMIEIPRACLLADRMAKTAQFFSFGT
                     NDLTQMTFGFSRDDINGFIHDYLDQKILAADPFQTIDQEGTGQLIEMAVKKGRATRTD
                     LKVGICGEQGGDPKSVEFCFKNGLTYVSCSPFRVPIARLSAAQASIKFGKTEKPAAKK
                     TAKKK"
     gene            complement(3458..4423)
                     /locus_tag="PLI16_06270"
     CDS             complement(3458..4423)
                     /locus_tag="PLI16_06270"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HOH84202.1"
                     /translation="MKKIFLLSLVFVAFICKVNAQSSVSVFCQEGEKFWLIVNGIKQN
                     ETSQTNVQVTGLTLPNYKLKVIFENSSLPAIDKTVFTQDADGKFTNASYEIKKDKKGQ
                     YVLRVSSFNDAPMAATQTYSAPLVIVERPATQTTQTTTTIISDNTQAEGGAVSINAVD
                     PITGQAINMNVSVNAGAMGATGGVSSSTTTTTTTTTTTTSSSGGVPVDTKPNHYIMTG
                     YNGPMGCPWPMNEGDFMAAKNSINSKSFDESKLTMAKQIVGSNCLLCSQVRDIMSLFS
                     FEATKLDFAKFAFNRVFDQGNYYKLNDAFTFESSIEELNNYINGR"
BASE COUNT         1098 a          958 c         1036 g         1418 t
ORIGIN      
        1 aatagttttg aaaaaatatt atgtttgtat aaacaattat gattcacccc ggcggcccgg
       61 gtctttaaat tctgcaaatc tatagcctgt aatctgaaat attatgcctt ataaacaaac
      121 ccccaccgaa aaaatttatt acaccattgg tgaagtggcc aaaatgtttg atgtgaacac
      181 atcgctgata cgtttctggg aaaaggagtt tgatatcatc aaaccatttc gcaacaaaaa
      241 agggaaccgt ttatttacaa aggccgatgt ggataatttt tacatcattt accacctggt
      301 gaaagaacgt ggctttacct tgcagggagc caaagacaag atgaaagaga acaaagtgga
      361 tactatcaac aatgtggagg tggtggcatc gctgaataag atcagggatt tcctgctcga
      421 actgaaagag gagttggatt gatataacgc aaaatgggaa agagaatttt ttaaaaaatc
      481 caggtaaaaa aaacagggca aattcatcaa tttgccctgc cttttttata ataaaacctg
      541 atggttttat tttttctttg ctgtcttttt tgcagccggt ttttccgttt ttccgaattt
      601 gatggaggcc tgtgctgctg acaatcttgc aataggaacg cggaaaggag agcagctgac
      661 ataggttaat ccatttttga aacagaattc aaccgatttg gggtcgccac cctgttcgcc
      721 gcagatacct actttcaggt cagtacgtgt ggcgcggcct ttcttcacag ccatttcgat
      781 caactggccg gtaccttcct ggtctatagt ctggaatgga tccgctgcca gaattttctg
      841 atccagatag tcgtggataa atccgttgat atcgtcgcgg ctgaagccaa aggtcatctg
      901 tgtcaggtcg ttagttccga atgagaagaa ctgggctgtt tttgccattc tgtctgccag
      961 caggcaagca cgtggtattt ctatcatggt gccatacagg tatttgattt cttttacctt
     1021 gtattttttg catacctcat cgtacacccg gtcagcaatt tttttggtaa cgtcaagttc
     1081 gcccacttcg caggtaacgg gaaccatgat ttcgggatgc ggatggtacc cttcgttgat
     1141 cagttcctgg gctgattcaa agatggcgcg gaactgcatt tcggatactt cgggataggt
     1201 aacgctgagg cgcacaccgc ggtgtcccat catagggttg ctttcgtgca ggccttcgcc
     1261 gcgtttggca atgtcggcga cactgatatt cagttcttta gccagttcgg cttgtttgtc
     1321 cggagtctga ggaacaaatt cgtgcaaagg cggatcgagc agacggaaag ttaccggcag
     1381 cttatccatg gccagcaggg tagcctttat gtctttcttt acaaaaggat acaactggtt
     1441 gagggctttt acgcgttctt ccctggagtt actcaagatc attttccgga gtgcgagcag
     1501 gggtttttct gatcctgcgc catagaacat gtgttcggtg cggaacaatc cgataccttc
     1561 agcgccaaaa tcacgtgctg tttttgcgtc agcagggttg tcggcattgg tacgtacgcc
     1621 cattttgcgg aaagagtcca ccattttcat aaaatgtttg aaaagagggt tttccgaggc
     1681 atccatgagt ttcaaggatt tctggtacac atggcctttg gttccgttga gtgttaccac
     1741 atcgccttct ttcagcacta tgttggtgcc ttctactttg gctgtttttg tctttacatc
     1801 cacatggagt tttccagcac ccactatgca gcacttaccc catccgcgag ccaccagggc
     1861 agcatgcgag gtcataccac cgcgtgcggt aaggatacca acagcggaac gcataccttc
     1921 cacgtcttcg gggttggttt cttcgcggat aagaattact ttttttcctt ttctttccca
     1981 ggctacggca tcttccgcag tgaatacaag ttgtcctaca gcagcgccgg ggcctgcggg
     2041 cagtcctttt acgagcgtgt ccactttctt ttcttctgcc gggtcgcaga tggggtgaag
     2101 cagttcgtcg agttgtgcag gatcaacacg taatacagca gttttttcgt cgatgagttt
     2161 ttctttcagc atatccatag ccattttcag ggctgcggta ccggtacgtt taccaacgcg
     2221 gcactggagc atatacaatt tggtttcctg aatggtaaac tcaatgtcga gcatatccct
     2281 gaagttcttt tccagaatgt tacggatctt gtcgagttcc ttataggttt tcggcatttg
     2341 ttcctgcatg ctatgcaggt gcttgttctg ttcgtttttt gtgtcgtcgt tcagggggtt
     2401 gggtgtacgg ataccggcaa ccacgtcttc gccctgggca ttgatgagcc attcgccata
     2461 gaacaggttt tcgccggtgg ccgggttgcg cgtaaatgcc acgccggtgg cagaagtgtc
     2521 gcccatattg ccaaaaacca ttgcctgtac atttactgct gtgccccagt cgtcgggaat
     2581 accttcgata cgacggtagg agacggcttt tttgccattc cagcttttga agacagccct
     2641 gatgccgcca aacaattgtt cttcggcatt atcaggaaat tcttttccaa attctttttt
     2701 tacttttttc ttgaattctc ctgctaaaac tttcaggtca tcggctgtga gttcggtgtc
     2761 ttgttttacc ttttttgatt tcttatattc gttgagcatg tcatcgagtt gcttacggat
     2821 atccttgtcg agaccttcgg ctttatccat aaccacatcg gcgtacatca taatcaatcg
     2881 tctgtacgaa tcccatacaa aacgtgggtt gttggttttt ttgatgagcc ccgggatcgt
     2941 ggcagtgcag agcccgatgt tcagtacggt gtccatcatt cccggcatag aagcgcgtgc
     3001 tcctgaacgg catgacaata ataatggatt gtttttatca ccatatttca ttcccatttc
     3061 agcttccact tttttcattc ctgcccatac ttctgctgtc agtgatttgg gcaactgaca
     3121 tttatttgca taataatcgg tacagcattc tgtagtaatt gaaaatcctg caggtacggg
     3181 taatcccagg ttaaccattt ctgcaaggtt ggcccctttg ccgccgagca aatttttcat
     3241 atcagctctg ccttcagctt tttttccgcc gaaataataa acatactttt tactttttgt
     3301 agccatttta ttgtcgtttt tgttaaaaaa tatatagaaa ttaatttgtt ttttatgatt
     3361 gggtgcaaag gtaattaaat aatttttcag agtacagaat aatccataaa aaaagccgga
     3421 cacccgtccg gcttttttat tggagaatga atttttttta tctgccgttg atataattgt
     3481 tgagttcttc aatagaactt tcaaaggtaa aagcatcgtt gagcttataa tagttgccct
     3541 gatcgaaaac acggttgaaa gcaaattttg caaagtcgag cttggtggct tcgaacgaga
     3601 ataaactcat aatatcgcgt acctgtgaac aaagcaggca gtttgatcca acaatttgtt
     3661 ttgccatggt aagtttgctt tcgtcgaagg atttcgagtt gatagagttt tttgctgcca
     3721 taaaatctcc ttcgttcata ggccaggggc atcccatcgg gccattgtat ccggtcatga
     3781 tgtaatgatt gggtttggtg tcaaccggaa ctccgccaga ggaagaggtg gtggtagtag
     3841 tagtggtggt ggtagtggtg gtggaagaag aaacgcctcc ggtggcgccc attgcaccgg
     3901 catttacgga tacattcata ttaatggcct gtcctgtaat aggatccaca gcattgatac
     3961 tcaccgcgcc gccttctgcc tgggtgttgt ccgaaataat ggtagtggtt gtttgtgtgg
     4021 tttgtgttgc tggcctttct acaattacca aaggagcgga ataggtttgg gtggctgcca
     4081 tgggcgcatc gttaaaacta gacactctta acacatattg gccttttttg tcttttttta
     4141 tttcgtagct ggcattggta aattttccat cggcatcctg ggtaaagact gttttatcaa
     4201 tagccggtaa actggaattt tcgaagatta cttttaattt gtaatttggc aaagtgagac
     4261 cggtaacctg cacattggtt tgcgaggttt cgttttgttt aatgccgttg acaattaacc
     4321 agaatttttc gccttcctga caaaaaacgc tgacactgga ctgtgcattg actttacaga
     4381 taaaagccac aaaaactaag ctgagtaaaa aaatcttttt cataaaatta cattgttaat
     4441 ggttaatatt tttgagcatt acttgcattg gttttttaca agcggtgtgc caaaattaat
     4501 caaaagagca
//
LOCUS       DAOSFP010000181         4508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17320_length_4508_cov_3.903660, whole genome shotgun sequence.
ACCESSION   DAOSFP010000181 DAOSFP010000000
VERSION     DAOSFP010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17320_length_4508_cov_3.903660"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="PLI16_06275"
     CDS             complement(<1..287)
                     /locus_tag="PLI16_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH84203.1"
                     /translation="MKNLKLIKSPLNLENPITVQVLGICSSLAVTAQLKPALVMALSV
                     TVVTAFSNLIISALRNTIPSRIRIIVQLVVIAALVIIVDQVLKAYVYDVSK"
     gene            complement(297..1016)
                     /gene="nqrC"
                     /locus_tag="PLI16_06280"
     CDS             complement(297..1016)
                     /gene="nqrC"
                     /locus_tag="PLI16_06280"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOH84204.1"
                     /translation="MFSNRYTLIYSAILVIVVAVALTIVAVMLKPAQTSNIRVEKMQN
                     ILKSVNMQSNAKNAEELYKKYITESFVITSDGTLKNDIKAFDVDLNLELKKKPEQRNL
                     PIFVFTGDEGNRYYIIPVRGKGLWGPIWGYLSFKSDFNTIVGTMFDHKGETPGLGAEI
                     NTPVFQEQFIGKTIFDEQMNFVSIKVIKGGAAPDDIHGVDAVSGGTITSQGLEAMIFD
                     GLTPYVTYFNKQMKQENSVTK"
     gene            complement(1026..2204)
                     /locus_tag="PLI16_06285"
     CDS             complement(1026..2204)
                     /locus_tag="PLI16_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOH84205.1"
                     /translation="MKGLRKYIDSIKPKFRKGGRFAKLESTFEAFETFLFVPKKVTSS
                     GSHIRDSVDLKRTMIIVVVALMPALIFGMWNVGYQFHKAMGTSVSILADFWFGFQAVL
                     PIIVVSYVTGLAIEFISAQIRHHEVNEGFLVTGMLIPLIMPVNIPLWVVAVGTAFAVI
                     FVKEVFGGTGMNIFNPALVARAFIFFAYPSEISGDSPWIFGFDNNLSGVDGITGATAL
                     SQAAAGHLESLPSLSDMFFGFIPGSIGETSKLAILLGALILIITGVGSLRIMLSTIAG
                     GVFMTLIFNLIGANNYMQIDPLTQLMMGGFMFGTVFMATDPVTSSQTASGKIVYGFLI
                     GVFAILVRVFNPAYPEGMMLSILLMNTFAPLIDHVVVQNNIRKRLKRLPKINTNVEPK
                     "
     gene            complement(2223..3569)
                     /locus_tag="PLI16_06290"
     CDS             complement(2223..3569)
                     /locus_tag="PLI16_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOH84206.1"
                     /translation="MQIIKIKKGLDIPMKGSAAKATKAFSMPEYVAIKPADFNNVLPR
                     LMVNPGDRVKAGTPLFKNKYDERLVFTSPVSGVVMEPVRGDKRLIMEIPVKADKNLVY
                     EEFGHHDISSMNAEDVRLLLIKSGLWTYIRQRPYDLIPAVDATPRDIHVSTFDSSPLA
                     PDYNYLLKDKLVFFQAGIDALARLCGKKVHLGLDGSTDNNIFSPVKNAELHYFKGPHP
                     AGNVGVQIHHTIPINKGESVWYLNPQDVAMIGKLFVDGIYDARRTIALTGPEAGKPAY
                     MEVLAGSKLKTLVEIPDQGQHLRIISGNVLTGDIVSTEGYLGYYHHQLTIISEGDYHE
                     FIGWAMPGFKKFSNSRLFFSWLFPGKKYDFDTNYHGGHRPLVMTGQYEKVFPFTILPV
                     YLVKAILAQDIEKMEQLGIYEVAPEDFALCDYVCTSKTEVQQIVREGLEMLRKEMS"
     gene            3963..>4508
                     /locus_tag="PLI16_06295"
     CDS             3963..>4508
                     /locus_tag="PLI16_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH84207.1"
                     /translation="MKVVVVGTGYVGLVTGTCFSEVGIDTTCVDIDVEKIENLKKGIL
                     PIYEPGLDTMIHRNVEKGRLHFSTDLASVLPDAEVVFIAVGTPPDEDGSADLKYVINV
                     ASEIGKNMEDYLLVVTKSTVPVGTAVKVKNAVQKELQARGVDIPFDVASNPEFLKEGA
                     AIDDFMKPDRIVVGLESERAEE"
BASE COUNT         1258 a          973 c          978 g         1299 t
ORIGIN      
        1 tttttactta catcatacac ataggctttc agcacctgat caacaataat taccagggcg
       61 gcaataacta ccagttgcac aataatcctg atgcgcgaag gaatggtgtt gcgcagcgca
      121 gaaataatca ggttagagaa agcggtaaca acggtaaccg acaatgccat taccagcgcc
      181 ggttttaact gtgctgttac agcaagggat gaacaaattc ccaaaacctg cacagtaatg
      241 ggattctcaa gatttaacgg ggattttatc agtttgaggt ttttcataat gatcacttat
      301 ttggtaactg aattttcttg tttcatttgt ttgttgaaat aggtaacata gggagtcagg
      361 ccgtcaaaga tcatggcttc caggccctgg ctggtaatcg ttccgcccga aacggcatcc
      421 accccgtgta tgtcatccgg cgccgctccc ccctttatta ctttgataga aacaaaattc
      481 atctgttcat caaaaattgt ctttccaatg aattgttcct gaaacaccgg ggtgtttatt
      541 tcagcgccga ggcctggagt ttcgcctttg tggtcaaaca tggttcccac tatggtattg
      601 aaatcgctct taaaagacag gtagccccaa attggtcccc ataggccttt ccctctcacg
      661 ggaataatat aataacggtt tccttcatcg ccggtaaaca caaaaatggg aagatttcgc
      721 tgctccggtt ttttctttaa ttcaagattc agatcgacat caaaggcctt gatgtcgttt
      781 ttaagcgttc catctgaggt tatcacaaac gattcggtta tgtatttttt gtataattct
      841 tcggcatttt ttgcattgga ttgcatattc accgacttca ggatgttttg catcttttct
      901 acccggatgt tgctggtctg ggccggtttc agcatcacag ccacaatggt cagggcgaca
      961 gcaacaacaa ttaccaaaat ggcggagtaa atcagcgtat atctgttact gaacataaag
     1021 tttggttatt tgggttcaac attggtattt atcttcggca atcttttcaa acgcttgcgg
     1081 atgttattct gcacaaccac atggtcaatc aatggcgcaa aagtgttcat cagcagtatg
     1141 gacaacatca tgccttcggg gtaggcaggg ttaaacaccc tgacgagtat tgcaaaaact
     1201 ccgatcagaa aaccatacac tattttgcct gaagcagtct gcgaggatgt tacggggtcg
     1261 gtggccataa acaccgtgcc aaacataaaa cctcccatca tcagttgtgt caggggatca
     1321 atctgcatat agttattggc tccgatcagg ttgaaaatca atgtcatgaa aacaccaccg
     1381 gctatggttg acaacatgat acgcaggctg ccaacgccgg tgattatcaa aatcagtgcg
     1441 ccaagtaata tggccagttt tgatgtctcg cctatagagc cggggataaa tccaaaaaac
     1501 atatccgata gcgatggcag cgattctaaa tgccctgcag cagcttgcga caatgctgtg
     1561 gcacccgtaa tgccatctac gccgctcagg ttattgtcaa aaccaaatat ccacggactg
     1621 tcgccactga tctccgaagg gtaggcaaaa aagataaagg cccttgccac cagggcgggg
     1681 ttaaagatat tcatccctgt acctccgaaa acttctttta caaaaatcac ggcaaaagcg
     1741 gtgcctacag caactaccca tagcggaatg ttaaccggca tgatcaaggg tatcagcatc
     1801 cccgtaacaa gaaatccttc attcacttca tgatgacgga tctgtgccga aataaattct
     1861 atggccaggc ctgttacata ggatactaca atgatgggca acacggcctg aaatccaaac
     1921 cagaaatcag ccagtatgct gacactggta cccatggcct tgtgaaactg gtagccaaca
     1981 ttccacatgc cgaaaatcaa ggccggcatc aatgccacca ccacaataat catcgtgcgt
     2041 ttcaggtcaa cagagtcgcg gatgtggctg cccgatgagg taaccttttt tggcacaaac
     2101 aaaaaagtct caaaagcctc gaatgtggac tccagctttg caaaccggcc tccttttcta
     2161 aactttggtt ttatggaatc gatgtatttt cttaaacctt tcatcgtgtg ataatttctt
     2221 tgttaactca tttcttttct tagcatttcc aagccttcgc gcactatttg ctgcacctcc
     2281 gtttttgaag tgcatacata atcgcataat gcaaaatctt cgggagccac ttcgtaaata
     2341 cccagctgtt ccatcttttc aatgtcctgg gcaaggatgg ctttcacgag gtaaaccggt
     2401 aaaatagtaa aaggaaatac cttttcatat tgccccgtca ttaccagtgg cctgtgacca
     2461 ccatggtaat tggtgtcaaa atcgtatttt tttcccggaa acaaccagct gaaaaacagt
     2521 cgtgagttac tgaatttttt gaatccgggc atcgcccagc cgataaattc atggtaatct
     2581 ccttcgctaa taatagtgag ctggtggtgg taatatccca aataaccctc agtgctgaca
     2641 atgtcgcccg ttaatacatt gccgctgatt attctaaggt gttgtccctg gtcagggatt
     2701 tcaacaagag tttttaattt gctgcctgct aaaacttcca tgtatgcagg tttgccggct
     2761 tcaggccctg ttaaagctat cgtgcggcgg gcatcgtaaa taccgtcaac gaataatttt
     2821 cctatcatgg ccacatcctg cggattaagg taccagacac tttctccctt gttgatgggt
     2881 atggtgtggt gaatttgcac gcccacattg ccggcaggat gaggtccttt gaaatagtga
     2941 agctcagcat ttttaaccgg agagaaaata ttgttgtcgg tggagccatc aagtcccagg
     3001 tgtacttttt tgccacacag cctggccaaa gcatcaatgc cggcctgaaa aaagaccaac
     3061 ttgtctttta gtaaataatt ataatccggt gccagagggg agctgtcgaa agtggaaacg
     3121 tggatatcgc gcggtgtggc gtccacggct ggaatgaggt cgtagggccg ttgcctgata
     3181 taagtccata aaccggattt aatgagcaac aaccggacat cttctgcatt catggaagaa
     3241 atatcatgat ggccaaactc ttcataaaca aggtttttgt ctgcttttac gggtatttcc
     3301 atgatcagcc gcttatcccc gcgtacaggc tccataacca ccccgctcac cggcgatgta
     3361 aagacaaggc gttcatcgta tttgttttta aacagcgggg ttccggcttt tacacggtcg
     3421 ccggggttta ccattaatcg gggcaataca ttgttaaaat ccgctggttt gatggcaaca
     3481 tattccggca ttgaaaaggc tttggttgcc tttgccgcgc tgcctttcat cggaatatca
     3541 aggccttttt ttatttttat aatttgcatg tatgttcaaa ttatgaattg actttggtga
     3601 gtgcaaaaat aaatattaaa ttacttaaat actaaattaa actggaataa agtaattttt
     3661 acatcaaata tacaacagaa aatatttcgg ggattttttt atttatactt gtttatataa
     3721 tcttttcaga tacagaatta tctgacaaat ttttttcctt ctaactttta aggtaaatga
     3781 ttgtggccgg tgaaaattac aaatacttgt gtttgttggt ctttgcaata ggcaagtaat
     3841 tattttttct aatgccgata tcataatatg ttggcctgaa aatattttag tccctcataa
     3901 agttctgtga tttaaagtgc agaatgttta tttttgcatg aaaaattaaa cttataaaca
     3961 ttatgaaagt tgtagtagta ggaacaggtt atgtcggttt ggttaccgga acatgttttt
     4021 ctgaagtagg catagatacc acttgtgtag atattgatgt agaaaaaatt gaaaacctga
     4081 aaaagggtat attgcccata tacgagccgg gtctcgatac catgatccac cgtaatgttg
     4141 aaaaaggccg cctgcatttt tcaacggatc tggcttcggt gctgcccgat gccgaggtgg
     4201 tatttatcgc tgtggggacc cctcctgatg aagacgggag cgctgacttg aaatatgtga
     4261 taaatgttgc ttccgaaata ggtaaaaaca tggaagatta tctgctggtg gttaccaaaa
     4321 gtaccgtgcc tgtgggtaca gcggtgaaag taaaaaatgc agtgcaaaaa gaacttcagg
     4381 cccgcggtgt tgatatccct ttcgatgtgg cttctaatcc ggagtttctg aaagaaggcg
     4441 ccgcaatcga tgattttatg aagcccgacc ggatagtggt tggcctggag tccgaacgtg
     4501 ctgaagaa
//
LOCUS       DAOSFP010000182         4476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17522_length_4476_cov_5.089799, whole genome shotgun sequence.
ACCESSION   DAOSFP010000182 DAOSFP010000000
VERSION     DAOSFP010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17522_length_4476_cov_5.089799"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..249)
                     /locus_tag="PLI16_06300"
     CDS             complement(40..249)
                     /locus_tag="PLI16_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOH84208.1"
                     /translation="MNRIKEVLDEKGIKQTWLAEQLGKSYNMVNAYVQNRQQPRIEVL
                     YDIAKILDVNVKDLLIDNDNKKNKK"
     gene            complement(745..1962)
                     /locus_tag="PLI16_06305"
     CDS             complement(745..1962)
                     /locus_tag="PLI16_06305"
                     /inference="COORDINATES: protein motif:HMM:NF033538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HOH84209.1"
                     /translation="MDTNIVTPAEVGDIFRLYGEQFRQCYNLTSRHIRAMNAIKNCRT
                     RALGSHSDTCDQCGYVRVYYNSCRNRHCPKCQGLNQTKWVDKLSANLLPVKYFHIVFT
                     IPAELNRLTLVNQESLYNILFKAASETILMLAKDKRHLGVETGLVAVLHTWGQNLTEH
                     PHLHILVPAGGWDQTTQYWKYSHKNFFVHVNVLSRVFRGKFISLLKKAYLEKKLKFEG
                     EIVPLKQANKFKQLLDLLYSKEWVVFCEKSQKSSSRIIEYLARYSYRVAISNDRIIGI
                     ENDRVFFRYKDYKDKGKRKVMELEAVEFIRRFMLHILPEGFCKIRYYGLLASRNRTSK
                     LRYIKKALGRKAIKAKYAGLSWNEMLYLLTGVDPLKCPICKTGKMMLLTGTSDVVTKS
                     KRAPPQKTNLFVL"
     gene            complement(1974..2807)
                     /locus_tag="PLI16_06310"
     CDS             complement(1974..2807)
                     /locus_tag="PLI16_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOH84210.1"
                     /translation="MKTLRKKMIQQMQLKGYSERTISTYILCISLLARYYNVSPDLIT
                     TEQIRAYIQHCLVDRRLSKAWLNQTISALKILHVEILKREWNRLDIPRPRREKTLPVI
                     LSREEVQKILNAHTNIKHRAFLTITYSAGLRMSEARHLKVSDIDSSRMLLRIEQGKGN
                     KDRYTILSPKALELLRVYWKVYKPRYWLFENSTHNPVPESTAQKIFKNAVEKVGVKKR
                     VSIHTLRHSFATHMLEQGVSLPIIQQLLGHKSLKTTSGYLHVQQYSIDAIRSPLDTLG
                     I"
     gene            complement(3212..3658)
                     /locus_tag="PLI16_06315"
     CDS             complement(3212..3658)
                     /locus_tag="PLI16_06315"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOH84211.1"
                     /translation="MEPLFYSDETFMKEALKEAQKAFDADEVPVGAVIVCNNRVIARA
                     HNLTERLNDVTAHAEMQAITAAANFLGSKYLTDCTLYVTVEPCVMCAGALFWSQISRM
                     VYAAGDPKRGYTMLNAVILHPKTKTQAGLMKEEAARMMEVFFKKKR"
     gene            3742..>4476
                     /locus_tag="PLI16_06320"
     CDS             3742..>4476
                     /locus_tag="PLI16_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOH84212.1"
                     /translation="MLRTHTCGELRLGAINTQVTLCGWVQRSRDLGGLTFIDLRDRYG
                     LTQLVFNMDVNAPLCQQARQLGREFVISVSGTVAERSNKNPKMDTGDIEIIVDTLTVM
                     NTSKLPPFTIEDNTDGGDELRMKYRYLDLRRAVNKNNLMLRHKMGVESRIYLNAQNFF
                     EIETPYLIKSTPEGARDFVVPSRMNQGEFYALPQSPQTFKQLLMVAGYDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMSFITRDD"
BASE COUNT         1219 a          966 c          878 g         1413 t
ORIGIN      
        1 ttggaacttt tatgtcgttc gttatttttt gttccttgct catttcttgt tctttttatt
       61 gtcgttgtca attaataggt ctttcacgtt tacgtccaat attttggcta tgtcgtaaag
      121 aacttctatt cgtggttgtt gtctattttg cacataagca tttaccatat tgtagctttt
      181 gcctaattgc tcagcaagcc aagtctgctt aatgcctttt tcatccaaca cttcttttat
      241 tctgttcatt tgttcaaatg atgtcaagtc gcaaagatag atttataatt tttaatatct
      301 cattttctaa gataaaaagt aatcagaaaa ttttcaacag tcttgtcggt gcgttggcaa
      361 aaaatggctc ttttggtttt gcgaagggtt ggcttgtgtg tcgggcaaaa ccaaatgtgc
      421 catttgcggg tcggcttttt acactgacgg ctaacgatta agattttatc cgcagtttgc
      481 gaaaaaaaat aatcgaagaa caaaagtaaa aataattgcg gataaaatca agttggactg
      541 agccgcttga actacagcca attataatga gtgaaattaa tattttcatt ttaactttat
      601 ttgatattca ttatcaacat taagttgaac tgacctgtaa ataaaattgt ttttcattca
      661 attctgatca tttttttgtt ttttcatttt caaacgaaca aagtaatcct ataccgcgta
      721 atgcagtaaa acagaatgat tatttcaaag aacgaataga ttggtttttt gcggtggcgc
      781 tcttttcgat tttgtaacta catcagatgt gcctgtaagt agcatcattt tccctgtttt
      841 gcatatgggg cattttagtg gatcaacacc tgtgagcaaa taaagcatct cattccatga
      901 taatcccgca tatttagctt ttatggcctt tctgcccaat gcttttttga tatacctaag
      961 tttagaagtt cgattgcgtg atgccaatag cccataatat ctgattttac aaaaaccttc
     1021 aggaagtatg tgtagcatga atctgcgtat aaattccacc gcttcaagtt ccataacttt
     1081 tcgttttccc ttatctttat aatctttata tcgaaagaaa acccggtcat tttcaattcc
     1141 aattatcctg tcgtttgata tggctacacg atatgagtat cgtgcaaggt attcaataat
     1201 gcgtgaagag cttttttgtg atttttcaca aaacacaacc cactctttac tgtaaagcag
     1261 gtcaagtagt tgtttgaatt tatttgcctg tttgagcggg actatttcac cttcaaactt
     1321 taacttcttc tctagatatg cttttttgag aagcgaaata aattttcctc tgaatacacg
     1381 ggacaataca ttaacatgaa cgaagaagtt tttatgtgag tatttccagt attgtgttgt
     1441 ctggtcccat ccaccggcag gaactaagat gtgcaaatga ggatgttctg tgaggttttg
     1501 cccccatgta tgtaaaacgg ccaccaatcc tgtttcaaca ccaagatgtc ttttgtcttt
     1561 tgcaagcatt agtatagttt cggatgctgc tttaaaaaga atgttataca gggattcctg
     1621 attaacaaga gtaagccggt taagctctgc tggtatggtg aaaacgatat gaaaatattt
     1681 tactggcaga agattagcag agagtttatc cacccatttt gtttggttta atccctggca
     1741 tttggggcag tgtctgttgc ggcaggagtt gtaataaaca cgaacgtacc cgcactgatc
     1801 acatgtatca ctatgactgc ctaatgcccg ggtacgacaa tttttaatgg cattcattgc
     1861 cctgatatgc cgactggtta gattataaca ttgcctgaat tgttcgccat ataaacgaaa
     1921 aatatctccc acctcggcag gagtcactat gtttgtatcc atctaatgtc gacctagatg
     1981 cctaatgtgt ctaaggggct tctgattgca tctatggaat attgctgaac atggagataa
     2041 ccgcttgtcg ttttgagtga tttatgtcca agcaattgct gaataatcgg caaagaaaca
     2101 ccttgttcca acatatgagt ggcaaagcta tgccggaggg tatgaatact tactcgtttt
     2161 ttaactccta ctttttctac cgcattcttg aaaatctttt gagccgttga ttcaggtact
     2221 ggattatggg tgctgttttc aaacagccaa tatctcggtt tatatacctt ccaataaaca
     2281 cgaagcagtt caagtgcttt tggtgataaa attgtatacc ggtctttatt ccccttgccc
     2341 tgttcaatgc gcaagagcat ccttgatgaa tcaatatctg agacttttaa atgtcgtgct
     2401 tcgctcatgc gcaggccggc tgaataagta atcgttagaa atgcccggtg tttaatattt
     2461 gtatgcgcat taagtatttt ttgcacttcc tcccttgata atattactgg aagtgttttc
     2521 tccctgcgtg gacgtggaat atcaagcctg ttccactcac gttttagaat ctcaacatgg
     2581 agtattttta atgcactaat ggtttgattc agccaggcct tgcttaatct cctatcaacc
     2641 aagcagtgct gaatgtatgc ccggatttgt tctgttgtaa tcaaatcagg cgaaacatta
     2701 taataacgtg ctaacaaact gatgcataaa atataagtgc ttatagttct ttcactataa
     2761 cccttcagct gcatctgctg aatcattttt tttcgtaatg ttttcatgac agtatgtttt
     2821 agtattacaa actgtcttta cgattgaaaa ggataaacgg ttgtttatgc tcgataaggc
     2881 cgccgccaaa ggcggtttag ttcaactact ttatatctac cacaaaacca tacaaattca
     2941 ccaatgtttt gatgaatatg catgataaat accattcaca ctatgaaatc aaaattagta
     3001 taatgtttta aataatttaa aaatgagaag aatttatttt cattaacctc agattgctca
     3061 ataggaacgt agtgaactat tgcgctccat atacgaaatc tcatgaccat tgggttaatc
     3121 caggctgata taagcgcgca ctccgcttca tcccggccgg ccatggcttt ttgtgaagtc
     3181 ggggagtagc ttctatatcc gcgctaaggg tttatctctt tttcttaaaa aaaacttcca
     3241 tcatccgggc cgcttcttct ttcatcaggc cggcctgtgt ttttgtcttc ggatgcaaaa
     3301 tgacggcatt caacatcgta tatccccgct tgggatcacc ggccgcatat accatcctgc
     3361 ttatctgcga ccagaacaag gccccggcgc acatcacaca aggctccacg gtaacgtaaa
     3421 gagtgcaatc ggtcaggtac ttgctcccca gaaaattggc ggcggcggtg atggcctgca
     3481 tctcggcatg cgccgtaaca tcattcagcc tttctgtgag gttgtgggca cgggctataa
     3541 ccctgttgtt gcacacgatt acagcaccca cgggcacctc gtcggcatca aaagcctttt
     3601 gtgcctcttt taaggcctcc ttcataaagg tctcgtcaga ataaaacagc ggttccataa
     3661 aattgttatc cacaaaaata atcaattccc ctgcaaaagg atacgctaaa tatgattaat
     3721 ttcgctctca aaatttttac catgttacga actcatacct gcggcgaatt acgactcggc
     3781 gcaatcaata cccaagttac cctttgcggc tgggtgcaac ggtccagaga cctgggcggc
     3841 ctcaccttta tcgacctgcg cgaccgctat ggcttaacac agcttgtatt taatatggac
     3901 gtaaacgccc ccctgtgcca acaggcccgt cagttgggac gcgagtttgt gatttccgtg
     3961 agcggcacag tggccgaacg cagcaacaaa aatcccaaaa tggataccgg tgatatcgag
     4021 attattgtcg ataccctcac ggtgatgaac acttcaaagc ttccgccttt caccatcgaa
     4081 gacaataccg acggcggcga cgaacttcgc atgaaatacc gctacctcga cctgcgtcgt
     4141 gccgtgaaca aaaacaacct gatgctgcgc cacaagatgg gtgtggagag ccgtatctac
     4201 ctcaacgcgc aaaatttctt tgaaatagaa acaccctacc tgataaaatc cacccccgaa
     4261 ggcgcccgcg attttgtggt gccctcgcgc atgaaccagg gcgagtttta cgccttgccg
     4321 caatcgccgc aaacgtttaa acagctgctg atggtggccg gttacgaccg ctattttcag
     4381 attgtacgtt gttttcgcga cgaagaccta cgggccgacc gccaacccga gtttacccag
     4441 atcgattgcg agatgtcatt tatcacccgc gacgat
//
LOCUS       DAOSFP010000183         4458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17616_length_4458_cov_4.477629, whole genome shotgun sequence.
ACCESSION   DAOSFP010000183 DAOSFP010000000
VERSION     DAOSFP010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17616_length_4458_cov_4.477629"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..511)
                     /locus_tag="PLI16_06325"
     CDS             complement(14..511)
                     /locus_tag="PLI16_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84213.1"
                     /translation="MKNFTLLVLLSAFLISCNKYEDGPSFSFQSAEKRLTGKWEVTQI
                     LFNEKDISAGYFSSNLNDFPVNIYSDWSKDYFIGISHTNGDVIAKSDLVLNKNKTTVT
                     FGLMTQPSYESLAGEIFSIIPALHDTDEWKILRLKNDELWISTDFDTNTYELHFNLLA
                     DFSDY"
     gene            complement(508..1452)
                     /locus_tag="PLI16_06330"
     CDS             complement(508..1452)
                     /locus_tag="PLI16_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOH84214.1"
                     /translation="MSEKTITLDLIDPVDFFGIRDHHLDQLKKHFSKLKIIARDNTIK
                     LLGDKGQIKAFEQLLDQLVNHYNNKGYISEEVIRQYLDPESILADNDSDSSNGLILHG
                     QRGLLIKARTPNQLRLTESSAANDLVFAVGPAGTGKTYTAVALAVKALRNKEVKRIIL
                     TRPAVEAGENLGFLPGDLKEKLDPYLQPLYDALWDMIPSQKLSLYLEERIIEIAPLAF
                     MRGRTLDNAFVILDEAQNATIPQMKMFLTRMGRSARFIVTGDITQIDLPKNQASGLVH
                     ALKILNNIPGIGIVNFDHSDIIRHKLITEIINAYSSKE"
     gene            1551..2327
                     /locus_tag="PLI16_06335"
     CDS             1551..2327
                     /locus_tag="PLI16_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOH84215.1"
                     /translation="MAIITLTSDWGLTDHYVAAMKGAIMCQSPGTAIVDITHSITKYS
                     LAETAFIIKNAYRNFPQGTIHIIGVNSDETKGAAHTLMAFDGHYFIGADNGVFSLLSE
                     NKPEKIIKINKLPEPKYNTFPSLDIYAVAACHIAEGKNPDDLGEPVAELYKLSGYLPQ
                     IEADSIKGIIIYFDSYDNAITNISDKVFNETGKGRRFSVFFRTEEINEIKKNYSEVGD
                     AEIVLVFGTTGLLEIALSKASARNLLGLSIYDSVRIEFEQ"
     gene            2519..2752
                     /locus_tag="PLI16_06340"
     CDS             2519..2752
                     /locus_tag="PLI16_06340"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOH84216.1"
                     /translation="MKNNMGLIDKAIRFVMAIVIIVLYATQTLPNDAWWSYLLLLLAA
                     FFVITSLLSFCPLYLPFKIDTRSHDIKKQESKK"
     gene            3131..3775
                     /locus_tag="PLI16_06345"
     CDS             3131..3775
                     /locus_tag="PLI16_06345"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOH84217.1"
                     /translation="METNKNNSAVNHQFKPLPYAYDALEPYIDKMTMEIHYSRHHKAY
                     FDNFIKAINGTPLEKMSMNELFENMSTLPAAVRNNGGGYYNHELFWENMAPGGVGMTE
                     RFEKMLKQQFGSVEKLKEEFANAGLTRFGSGWAWLNVDEKGNLFLSSTPNQDNPLMDV
                     AEKRGTPVLAMDVWEHAYYLKYQNKRVDYINAFWNIINWKVVEERYDEAVVKNK"
BASE COUNT         1360 a          815 c          937 g         1346 t
ORIGIN      
        1 ggttgatttt attttaataa tcagaaaaat cggctaaaag attaaaatgc agttcgtaag
       61 tgttggtgtc aaaatctgtg cttatccaaa gttcgtcatt tttcaggcgc aatattttcc
      121 attcgtcggt atcatgcagg gccgggataa tgctgaaaat ctcccctgca agcgattcgt
      181 atgatggctg cgtcattagt ccgaaagtaa ccgtggtttt gtttttattc aaaaccaggt
      241 cggatttggc tataacatca ccgttggtat gcgaaatgcc tataaaataa tctttactcc
      301 aatccgaata aatatttacg ggaaagtcat ttaaattact ggaaaaatag cctgcagaaa
      361 tgtctttttc gttaaaaagt atttgtgtca cttcccattt tcctgtcagt cttttttctg
      421 cagactggaa actgaatgat ggcccatctt catatttgtt gcatgaaatc aaaaaagcag
      481 agagtaagac cagtagagta aaatttttca ttcttttgac gaataagcat tgatgatttc
      541 agtaataagt ttatgacgaa taatatcgga atggtcgaag tttactatgc cgataccagg
      601 tatgttgttc agtattttaa gtgcatgaac aagtccagaa gcctgatttt ttggcaggtc
      661 tatctgggta atgtcgccgg ttacaataaa tctggcagaa cggcccatac gcgtgagaaa
      721 catcttcatc tgcggtatgg tggcattttg tgcttcgtcg aggatgacaa aggcattatc
      781 gagggtgcgg ccacgcataa aagccagtgg ggcgatttcg atgatgcgtt cttccagata
      841 aagactgagt ttttgtgagg gtatcatgtc ccacaaggca tcgtaaagag gctgaaggta
      901 ggggtccagt ttttctttga ggtcgccggg taaaaagcca aggttttctc cggcttctac
      961 tgccggacgg gtaaggatga tacgcttaac ttctttgttg cggagcgctt ttacggccag
     1021 tgccactgct gtataagtct tgcctgtacc ggcaggtccc acggcaaata ccaaatcgtt
     1081 ggctgcactg ctttcggtga gacgaagctg atttggcgta cgcgctttga tcagcagccc
     1141 tctttgcccg tgcaatatca agccgtttga actgtcactg tcattatctg ccaggatgct
     1201 ttccgggtca aggtattgcc tgataacctc ttccgaaata tagcctttgt tgttatagtg
     1261 attgacaagt tgatccagca actgttcaaa agcctttatc tggcctttat cgccgaggag
     1321 tttgatcgtg ttgtcgcggg caatgatctt aagtttggaa aaatgctttt tcagctggtc
     1381 gaggtgatga tcacgaatgc caaaaaaatc tacagggtca attaaatcca atgttatggt
     1441 cttctcgctc atcaaatggt attaaccgga taaaatttta acaaaattaa taaaaattgc
     1501 gttgccgcag tcgaaaattt gtaattttga aagcgtataa ttaacataaa atggcaatta
     1561 ttacattaac gagcgattgg gggttgactg atcattacgt ggcagccatg aaaggtgcca
     1621 tcatgtgtca atcgcctgga accgccatcg tggatattac gcacagcatc accaagtaca
     1681 gtctggccga aaccgccttt ataatcaaaa atgcttaccg taattttcct cagggtacca
     1741 tccatatcat cggtgttaat tctgacgaaa ctaaaggggc tgcgcacacc ctgatggctt
     1801 ttgacggtca ttattttatt ggcgccgaca atggggtgtt ctcgttgctt agcgaaaaca
     1861 aacccgaaaa aatcattaag atcaataaac ttcccgaacc gaaatataat acattcccgt
     1921 cgctggatat atacgctgtg gcggcctgcc atattgccga aggaaagaat cccgatgacc
     1981 tgggcgagcc tgtggcggaa ctctacaaac tttcgggcta tctgcctcag attgaagccg
     2041 actccataaa aggtatcatc atttattttg attcgtatga taatgccatc accaatattt
     2101 ctgataaggt gtttaatgaa acgggcaaag gacggcggtt ttcggtattt ttcaggaccg
     2161 aagaaataaa tgaaataaag aaaaactatt ccgaagtggg tgatgccgaa attgtactgg
     2221 tatttggcac caccggcctg cttgaaattg cgcttagtaa agccagcgcc cgcaatttgc
     2281 tcggattgag catctatgat tcggtcagga tcgaattcga gcaataatat tttgcatttt
     2341 tttgactttc tctttgcctt gattctgagg catgggtatt ttttgctgta actaataaat
     2401 aaacggaaag gccgaatgta tcataaaaat atgagaaata acttgatttt taaatgaaaa
     2461 ttaagtatat ttgtttgtga aaagtcggat aaattattaa acaattaata ataattatat
     2521 gaaaaataat atgggactca ttgataaggc tatcagattc gttatggcta ttgtcatcat
     2581 agtattgtat gctacccaaa ccctgcccaa tgacgcatgg tggagttatt tactgctttt
     2641 gctggccgct ttttttgtga ttaccagctt gttgagtttc tgtcctttgt atctgccttt
     2701 caaaattgat acacgttcgc acgatattaa aaagcaggaa agcaaaaaat aactgaactt
     2761 tctttcttta acccaaatta cacatttgat agtgtaatcg gtaaaatgct tgtagtcaat
     2821 ccctgtagcc agggcttgat acaattacaa aatccggatt aacacaacta agtcagctag
     2881 ttcggctcat tctttgcctt ctgctaaata attttgtttt aaactgtcgt caataagtta
     2941 gaattaacgc agagtttttt tatgacgtgt tgtcatgttt tttttggcat agttattgtt
     3001 atttaaaaac agtctaaata aaaataaaat gaaaaaaaca ctttttgcag cctttacaat
     3061 gacaatattc tcattgggac tgggcgctca agaaatcaca acaattatta accaacaaaa
     3121 acaaaaaaca atggaaacaa ataaaaataa cagcgcagta aatcaccaat tcaagccatt
     3181 accttatgct tatgacgctt tagagcctta cattgataaa atgaccatgg aaatccatta
     3241 ttcacggcat cacaaggcgt acttcgataa ttttatcaaa gctattaacg gcacaccttt
     3301 ggaaaaaatg tcaatgaacg aactttttga aaacatgagc acattgccgg cagctgtgcg
     3361 caacaacgga ggcgggtatt ataaccatga acttttctgg gagaatatgg ctcccggcgg
     3421 agtaggcatg accgaacgct ttgaaaaaat gctgaaacag cagtttggct ctgtcgaaaa
     3481 gctgaaagaa gagtttgcca atgctggcct gacacgtttt ggcagcggct gggcatggct
     3541 gaatgtggat gaaaaaggaa atttgttttt aagttcgaca cccaaccagg ataacccttt
     3601 gatggatgtt gctgaaaaaa gaggaacacc ggtactagcc atggatgtgt gggaacatgc
     3661 ctattacctc aaatatcaga acaaacgggt cgattacatc aacgcttttt ggaacatcat
     3721 caactggaaa gtagtcgagg aaagatatga cgaggcagtg gtgaagaata aataatgttg
     3781 gctggaaaac ccttatttgc ataagggtgc atctaacaac attgatcggt caggtctgaa
     3841 aacaataaag tcccaggcgg ccgaagtcct gaaaaactgc ggtatactga gcgtatatga
     3901 ggatttgaca atcgaaggaa aacaaacaaa tgtgaagttt ttagaggtgc ccataaagaa
     3961 aacgccggtt tcaacctaaa ggaaggaatc ggcttttttg tttatatagt atttattaac
     4021 ctgagttcgg gttaaaaata ttgtgtcttt actccgttag gagttaaata tcaatagaaa
     4081 agaaatacaa aaaacattta ttctccgtta ggagattaac gttttaataa tgtttatgtc
     4141 ctaacggaca aaatattcta cacgtttttg tttttctatt gatattaatt gcctacggca
     4201 aatattaaca gattaataat tagtgttttg acaaaatagc attgatttat ttatcccgaa
     4261 ctcatgttat tagtaacttt ttttgtggtt gaaaataatg tcaaaatctg acaaaatgta
     4321 atgtggcccg atattttcaa cccgaaaatg ttgttcggga caaattattc aggtgaagca
     4381 atctgatatt attccgaaat tattatttcg tcttatccgg aaatgcaggt atcggcggca
     4441 gtttattgtc ggtttgtt
//
LOCUS       DAOSFP010000184         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17835_length_4420_cov_4.468270, whole genome shotgun sequence.
ACCESSION   DAOSFP010000184 DAOSFP010000000
VERSION     DAOSFP010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17835_length_4420_cov_4.468270"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PLI16_06350"
     CDS             complement(<1..999)
                     /locus_tag="PLI16_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOH84218.1"
                     /translation="MKNKDILQMLDGAPCGIFFTDRTLTITFWNDDMRRMTGLDRLDA
                     LHKKLSLLTFYDIRHNEKKPLDFRQCLKGEKANIAKTAFLEGKDGKQILVFINAGFIQ
                     AGTEPMLFVAVTDISKEITCSTVAAVPLIVQEKEALQKIVGHDDKIRELYRLIELAAD
                     SMATVNISGESGTGKELVASAIHQLSPRKDKPFIKVNCSALTETLLESELFGHVKGSF
                     TGAYKDKTGKFEAAGGGTIFLDEIGEISQMIQVKLLRVIQEKTIEKVGDNKPVKVDMR
                     IITATNRNLRELVSKGLFREDLFYRLNVFPIVTAPLRDRMNDIPLLVEHFIAKFNRQ"
     gene            1183..1515
                     /gene="trxA"
                     /locus_tag="PLI16_06355"
     CDS             1183..1515
                     /gene="trxA"
                     /locus_tag="PLI16_06355"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH84219.1"
                     /translation="MFSKNKNVINLTAETFQQAIKEKLTLVDFWAEWCVPCRIQSPIL
                     EEVAAEMKDRVTIAKLNVEKFRDIASHYRISSIPTMLLFRNGKMIKQFVGIQQKQTLL
                     NTFDRMFI"
     gene            1697..2017
                     /gene="trxA"
                     /locus_tag="PLI16_06360"
     CDS             1697..2017
                     /gene="trxA"
                     /locus_tag="PLI16_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOH84220.1"
                     /translation="MAGVLILTDNSFDEKIKKGLVLVDFWATWCRPCRIQAPILDEVS
                     ALMNGKAVIGKLDIDQNPKIRDRYFIQSIPTMLLFKDGKVVKTFVGVTSKEVIMAEMN
                     KLIK"
     gene            2093..2422
                     /gene="trxA"
                     /locus_tag="PLI16_06365"
                     /pseudo
     CDS             2093..2422
                     /gene="trxA"
                     /locus_tag="PLI16_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
     gene            2494..2703
                     /locus_tag="PLI16_06370"
     CDS             2494..2703
                     /locus_tag="PLI16_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOH84221.1"
                     /translation="MKEFRGSIIGVIVGGIGGYLYYRLVGCSTGACPITSNPWMSILW
                     GIALGYLLGETFNKNKKKTQNHEGA"
     gene            2690..3094
                     /locus_tag="PLI16_06375"
     CDS             2690..3094
                     /locus_tag="PLI16_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOH84222.1"
                     /translation="MKEHNIFIEHSSGFDLLTTVEKIITTAAAKSWQNPAVHNLQQSL
                     AKAGKTVRPVQIIEICKPEFSAKILEKNDERIASVMMPCRIAVYEKDDGKTYVSLLNM
                     EAMAPGLPQAITESVGNATIESHEIVKTALGL"
     gene            3166..3348
                     /locus_tag="PLI16_06380"
     CDS             3166..3348
                     /locus_tag="PLI16_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH84223.1"
                     /translation="MALSVTKIKCPQNHRCPLITMCPADAITQTGTGLPVIDNTKCIN
                     CGRCLKFCPTGAVERK"
     gene            3457..4077
                     /locus_tag="PLI16_06385"
     CDS             3457..4077
                     /locus_tag="PLI16_06385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH84224.1"
                     /translation="MMNYFDKRAREWDDNPMHAERSAAIAEKIIEHLPATPVMRAMEY
                     GAGTGILSFMLRDYFDEIVLLDSSREMLKVTGDKIIAAGAVNLKTRFFDLEKEELTDE
                     KFDVIFTQMVLHHIEDVTLILKRFSTLLNEGGIVAIADLYPEDGTFHGEDFKGHKGFD
                     VNQFAQWLTKTGFAEIHHRPCFSIKKQIGNETKEYPVFLITAKSNA"
     gene            4153..>4420
                     /locus_tag="PLI16_06390"
     CDS             4153..>4420
                     /locus_tag="PLI16_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84225.1"
                     /translation="MNEPLYIFLSGIDLQTALLYFVRIIEKLMVIYFTLYFLIDIFLF
                     IYSLVLHHRNKVRKISSVNYYKHSVSIIVPAYNEEVSLVSCIKML"
BASE COUNT         1385 a          933 c          925 g         1177 t
ORIGIN      
        1 ctgccggtta aactttgcaa taaaatgttc caccagcagc ggaatgtcgt tcatacggtc
       61 acgcaggggt gcagtaacaa tcggaaatac gttcaggcgg taaaacaggt cttcgcggaa
      121 caagcctttg ctgacaagtt ccctgaggtt tctgtttgta gctgtaatta tgcgcatgtc
      181 cactttcaca ggtttattat cgccgacctt ttcgatggtt ttttcctgaa tcacgcgcaa
      241 cagtttaacc tgtatcatct ggctgatttc tccaatctcg tccaaaaaaa ttgtgccgcc
      301 tcctgccgct tcgaacttac cggttttgtc tttataggct ccggtgaaag agcccttaac
      361 atggccaaac aattcgcttt ccagcagtgt ttcggtcagc gcggaacagt ttacttttat
      421 gaagggtttg tctttcctgg gagacaattg gtgaatggcg ctggccacca gctcctttcc
      481 ggtgccgctt tcgccggaaa tattcaccgt agccatcgaa tcggccgcta attcgatcag
      541 gcggtacaac tcacgtattt tgtcgtcgtg gccgacaatt ttttgcaggg cttccttttc
      601 ctgaacaatc agcggaaccg cagcgacagt gctgcatgta atctcttttg aaatatcggt
      661 aacggccaca aacaacatcg gctccgtgcc cgcctgaatg aacccggcat taatgaaaac
      721 caaaatttgc tttccgtctt ttccctccag aaaggccgtt ttggcaatat ttgccttctc
      781 tcctttcagg cactgcctga aatccaatgg ctttttttca ttgtgccgga tgtcataaaa
      841 tgtcagcagg gaaagctttt tgtgcaaggc atcaagtctg tccagcccgg tcatgcggcg
      901 catatcatca ttccagaaag taatggtcag cgtacggtct gtaaaaaaaa tgccgcaggg
      961 agctccgtca agcatttgta agatgtcttt atttttcata ccgaaaaaag ttaattaact
     1021 taattaatgc aaagttaagt ttttatttta aaagttaatc attcataaac catctttttt
     1081 tctcatgctt caagatattc attttaatat cctgatttat aaccacttaa aaactttggc
     1141 acaactattg agcaacaggt tcagtacaaa aaaaataatg ttatgttttc aaaaaataaa
     1201 aacgttataa acctgacagc cgaaactttt cagcaggcga taaaggagaa actgaccctg
     1261 gtggatttct gggcagaatg gtgtgtcccc tgccgcatac agtcgcccat actcgaagag
     1321 gtagctgccg aaatgaaaga tagagtgacc attgcaaaac tgaacgttga aaaattccgc
     1381 gacatcgctt cgcattatcg catcagcagc atccctacga tgttattgtt cagaaacggc
     1441 aaaatgataa aacagtttgt aggcatacaa caaaaacaaa ccctgctaaa cactttcgac
     1501 agaatgttca tataaatcaa taaaatcaac aaacaagaaa aacatgaaac acgtaaaaaa
     1561 atactcctgg gttttaatca ctgcaacagt ggtcgtagcc gccctgacga tttatgaatg
     1621 cacggcagga aaagccacga atgcggcgac tcccggccaa aaaaaggccg tgacagtatc
     1681 cgctgtctct gaaaatatgg ccggcgttct gattttgacc gacaattcat ttgatgaaaa
     1741 aatcaaaaaa ggattggttt tggtagattt ctgggcaaca tggtgcaggc cttgcaggat
     1801 acaggctcca attctcgacg aggtaagcgc cctgatgaac ggaaaagccg tgataggtaa
     1861 attagatatt gatcaaaatc caaaaatcag agaccgttat tttattcaaa gcatacctac
     1921 catgctgttg ttcaaagacg gcaaggtggt aaaaacattt gtcggcgtta cctctaagga
     1981 agtaataatg gccgaaatga acaaattaat caaataaata aaaaactaat cattcataac
     2041 aatctaaaaa cataaaaaaa tggaacattt aacagctgaa acttttaaac aaaaggtatt
     2101 taattacgaa atcaaccagg aatggaaatt tgaaggcaac aagccatgta tcatcgactt
     2161 ttacgccgat tggtgcggcc cctgcaagat ggtggcgccg gtattggaag aactgtccaa
     2221 agaatacgaa ggcaaaatcg atatttacaa aatagacact gaacaggagc aggaactggc
     2281 cggagaattt ggcatacaga gcattccctc catccttttc gtaccgatgg aaggccagcc
     2341 acagatgacc atgggtgcta tgcctaaaca atcgtttata caggtgattg acgaggtgtt
     2401 gctcaacaaa aaacaaaatt aaaaccctcc ggacaaacca tggaagataa ccaggaaaac
     2461 aaagcgcctt cccgcgagcg tatttttgta aagataaaag aatttcgggg atccatcatc
     2521 ggggttatcg ttggcggtat tggcggatat ctgtattacc ggctggtggg ctgctcaacg
     2581 ggcgcctgcc ccattacctc caacccctgg atgagcattt tgtggggcat cgctcttgga
     2641 tacctgttgg gagaaacatt taataagaat aaaaagaaaa cacaaaatca tgaaggagca
     2701 taatattttt atagaacact caagtggttt cgacttgctg accaccgtcg aaaaaattat
     2761 cacaaccgct gcagcaaaat cctggcaaaa tccagctgta cacaacctgc aacaatcgct
     2821 ggccaaggcc ggaaaaacag tcagaccggt acagataata gaaatttgca aaccggaatt
     2881 ttcggcaaaa attcttgaaa aaaatgatga aaggatagcc tcggtgatga tgccgtgccg
     2941 cattgcagta tatgaaaaag atgatggcaa aacctatgtc tccctgctca acatggaggc
     3001 catggccccc ggcttgcccc aggcgataac agaatctgtt ggtaatgcta ccatcgaatc
     3061 gcacgagatt gtaaaaacgg ctttgggttt gtaaagaaaa agccgggatt tttcagcatt
     3121 attttttcaa aaaaaatttt ttccgacata aaaaagaaaa acaaaatggc cttatctgta
     3181 actaaaataa aatgcccgca aaaccaccgt tgtccgctta taaccatgtg tccggccgat
     3241 gccataactc aaacgggtac cgggttgccg gttattgaca atacaaaatg tatcaattgt
     3301 ggccggtgcc tgaaattttg ccctaccggc gcggttgaaa gaaaataagc gttccgttat
     3361 ggcccggccc tgccattagt atgccataag cgacagtatg ttaaaatcgg atatggatgg
     3421 gttattgctg cgtatatttg cattaattta tttctcatga tgaattattt tgataaaaga
     3481 gccagggagt gggatgacaa cccgatgcac gccgaacgtt cggcggccat cgccgaaaaa
     3541 atcatagaac acctaccggc aacccctgtg atgagagcaa tggagtatgg ggccggcacc
     3601 ggcattttaa gttttatgct gagggattat tttgatgaaa tcgtattgct tgacagttcg
     3661 agagaaatgt taaaagtaac cggcgacaaa ataattgccg caggcgccgt caacctaaaa
     3721 acacgttttt tcgacctcga aaaagaggaa ctgacggatg aaaaattcga tgtgattttt
     3781 acacagatgg tgctgcatca catcgaagat gtaacactaa tacttaagcg tttttcgacc
     3841 ttattaaacg aggggggaat tgttgctatt gctgatctct atccggaaga tggaactttt
     3901 cacggggaag attttaaagg gcataaaggg tttgatgtaa atcaatttgc ccaatggctt
     3961 acaaaaaccg gctttgccga aatacatcac cggccatgtt tttcaatcaa aaagcagatt
     4021 ggcaacgaaa cgaaagagta tccggttttt ttgatcacgg caaaaagtaa tgcataacaa
     4081 attttcatga ataaaaaatg gattttttaa aatcccggcc catgtaataa taagcaaact
     4141 gacgacaaat aaatgaacga accgttgtat attttcttat ccgggattga ccttcagacg
     4201 gcattactat attttgtccg gataattgaa aaactgatgg tcatttattt cacattgtat
     4261 tttcttattg acattttttt gtttatatat tcactggtat tacatcaccg gaacaaagtc
     4321 agaaaaattt cttccgtcaa ttattacaag cattccgttt ccattatcgt tcctgcctat
     4381 aacgaggaag tttcgctggt ttcgtgcata aaaatgcttt
//
LOCUS       DAOSFP010000185         4418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17845_length_4418_cov_4.152876, whole genome shotgun sequence.
ACCESSION   DAOSFP010000185 DAOSFP010000000
VERSION     DAOSFP010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17845_length_4418_cov_4.152876"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLI16_06395"
     CDS             <1..373
                     /locus_tag="PLI16_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH84226.1"
                     /translation="RTLKKHHIANNVFTVTDGVQALDYLFHRGSYSARENGDPRVIFL
                     DIKLPKINGLEILKQIKADNNLNHIPIVMLTSSAEDPDIKTAYNLGANSYVVKPVDIN
                     SFTETINNLGMYWLVINQPPK"
     gene            381..3767
                     /locus_tag="PLI16_06400"
     CDS             381..3767
                     /locus_tag="PLI16_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH84227.1"
                     /translation="MNNHYKILIVEDNPLDAELARREIRQVLKSVEFVELCTENKFLE
                     SLENFKPDIIVSDFSMPNFDGMSVIKLAKKHAPLIPVIIFTGSLNEETAAETVKVGAV
                     DYVLKENNIRLRQAIQYALEKKQMWRERLEAEEKLRLSEERYRVLFELAADGILRVSA
                     EGKIIDINHSGLQLSGYNKTEVLQMHISEFFTKKELEKKPIRYDLLQKGHALKIERTF
                     VRKDGSTCIVEMHSKMMPDNTIQSIFRDITERKNIEMDLRESEEQLSQLIQTLPIGVV
                     IHNKGKVVFANEATFNIMEAQPDISIDGLNIFNFIAPEYHEMASQRIRNSIKGNQTEQ
                     VEEQFITLKGNQIAAEVKTLPILFKGEKNLLSVFSDITARKQAELHLKESEAKYRSFF
                     ENTGTAIAISEEDTTLSLVNEKYAELVGLPKEEIENKKSFLDFMVPEDIENSMKIHSL
                     RLKEPKKAEKCYELGFKNVRGEVRNMLVYTDIIPGTNKSLASLLDITERKIAEEELRK
                     NRMFLRTMIDSLPIWISAVDTQGNYVIANKYYESFFNKPLELIEGHNFKELFTEQDYQ
                     RHNALVDKCLSTGKSIEVSETITGGNNITAHIFGTYTPLFDEKGKIFAMAVLAIDISK
                     QKLAEIALKESETRFRSTFEGAPIGMCLNFTDGKFFEANNAFCQMIGYSIEELRSRNF
                     IDITHPDDIEISKQFISDILQLEGSDSKSIEKRYLKKDGSLIWVITSVSLYRDTNDNP
                     KYFIVQILDITEQKLTEQKLIEAKNQLEVVFNNGPDAILLTHIEDGHYIDSNKTFTRY
                     TGYTPDDLRGKSTLDIDIWGNPDDRNRMIKEVKEKGFCHNFELEIKNKDRTTRVIEMS
                     AEVILIKGKPHLLTISRDIAERKKMEELLKKQNEELHELVSTKDRFFSIIAHDLKDPH
                     NAILGFSEILAGSYDILSDQDRKNYIVNILHSSQNLSRLLRNLLEWANSQTGRMIYKP
                     ESINISAVVQETMDILLYQAKIKQIRLIPDISSSHEVFADPNMVRTILRNLVSNAIKY
                     THRNGFVKISTSSVTDKKHKTDFLEISVIDNGIGIKKENIDKLFKLDHKVKTPGTEEE
                     MGSGLGLILCYELVRKNNGYLNISSEENVGTTVTFALPKK"
     gene            3774..4361
                     /locus_tag="PLI16_06405"
     CDS             3774..4361
                     /locus_tag="PLI16_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOH84228.1"
                     /translation="MKFEFSDKNALTQIVEAISNHYCQGMVIVDEASNTDKLPEEMND
                     LPDPVKQFFRENGIRMCRLMNSLPSPFFIKDSKANWFYVNEHFCKFFNVNSKDIFGKN
                     SFCIFPKNQAEQFFRQDIELLENGKDSQVEIVVYNADGSNRTILSKKTLFRDINDQPY
                     IVGLFHEITGLKDIETSIKQEKTELEAKVKERTSY"
BASE COUNT         1567 a          928 c          874 g         1049 t
ORIGIN      
        1 acgaaccctg aaaaagcacc atatcgccaa caatgttttt acggttacag acggggtgca
       61 ggcgctggat tatctttttc accgcggcag ctattctgcc agggaaaatg gcgacccaag
      121 agtaattttc ctcgacatca agctcccaaa aatcaacgga cttgaaatac tcaaacaaat
      181 caaagccgac aataacctga accacatccc gattgtcatg ttaacatcgt cagccgaaga
      241 ccccgacatc aaaaccgctt acaacctggg agccaacagc tatgtcgtca aacctgttga
      301 catcaacagc tttaccgaaa ccatcaacaa cctgggaatg tactggcttg taatcaatca
      361 accaccgaaa taactttacc atgaacaacc actataaaat tcttattgtt gaagacaatc
      421 ctctggatgc tgaacttgcc cgaagagaga tacggcaggt tttaaaatcc gtagaatttg
      481 ttgaattatg tacagagaat aaattccttg agtcgcttga aaatttcaaa cccgatatca
      541 tcgtctcaga tttcagtatg ccgaattttg atgggatgtc ggtgataaag ctggcaaaaa
      601 aacacgcgcc cctgattcct gtcatcattt ttacaggctc attgaatgaa gaaaccgctg
      661 ccgaaacagt aaaagtcgga gccgttgatt atgttctgaa agaaaacaac atccggctgc
      721 gacaagccat tcaatatgcc ctggaaaaaa aacaaatgtg gagggagcgc ctcgaagctg
      781 aagaaaaact ccgccttagc gaagaacgct acagagtgtt attcgaactc gctgccgacg
      841 gcatattaag ggtttctgcc gaaggaaaaa taattgatat caaccatagc ggactgcagt
      901 tgtccggata caacaaaacg gaagtgctgc aaatgcatat ttcggaattc tttacaaaaa
      961 aagaactgga aaagaagcca atccgctatg acctgctcca aaaaggccat gccttaaaga
     1021 ttgagaggac atttgtccgt aaagacggaa gcacctgtat tgtggagatg cactcgaaaa
     1081 tgatgcccga caacactatt caaagtattt tccgcgatat taccgaaaga aaaaatattg
     1141 aaatggattt gcgtgaaagt gaagagcagc taagccaact tatacagaca cttccgatag
     1201 gagttgtcat tcataacaag ggcaaagttg tttttgccaa tgaggcaacc tttaatatta
     1261 tggaggcgca gccggatatt agtattgacg gattgaatat ctttaatttt attgctcctg
     1321 aataccacga gatggcttcc caaagaataa gaaattcaat aaaaggaaac caaacagaac
     1381 aggtagaaga acaattcata actttaaaag gcaaccagat agctgccgag gtaaagacac
     1441 tgcctattct gttcaaaggc gaaaaaaact tgctcagcgt attcagcgac attactgcac
     1501 gtaaacaggc agaattgcat ttgaaagaat cggaagccaa atacaggtct ttcttcgaga
     1561 atacaggtac tgccattgcc atcagtgaag aagacacgac cctgtcgctg gtaaatgaaa
     1621 aatatgccga attggttggc cttccaaaag aagaaattga aaataaaaaa agctttttag
     1681 attttatggt tcccgaagac attgaaaatt caatgaaaat ccattctctc cggttgaagg
     1741 aacccaaaaa agcagaaaaa tgttacgaac tcggattcaa aaatgtcagg ggcgaggtaa
     1801 gaaacatgct ggtttatacg gatatcatcc cgggcacaaa taaaagcctg gcttctttgc
     1861 tggatattac cgagcgcaaa atagccgaag aagagctgcg aaaaaacagg atgtttttgc
     1921 ggactatgat agattcttta ccaatatgga tctcggccgt tgatacacag ggcaactatg
     1981 ttattgccaa caaatactat gaatcatttt ttaataaacc tctggaactc atcgagggcc
     2041 ataattttaa agaattgttt acagaacagg actatcaacg gcacaacgcc ctggtagata
     2101 aatgccttag taccggcaag tccattgaag tatcggagac aataacaggc ggaaataata
     2161 tcacagcaca tattttcggc acatataccc ctctgtttga tgaaaagggt aaaatcttcg
     2221 cgatggctgt actggcgata gatattagca agcaaaaatt agcagaaata gcattgaagg
     2281 agagcgaaac cagattccgt tcgacatttg aaggcgcacc catcggcatg tgcctgaatt
     2341 ttaccgatgg aaaatttttc gaagcaaata atgccttctg ccaaatgatt ggatatagta
     2401 tagaagaact ccggtcacgc aatttcattg atattacaca cccagatgat attgaaatct
     2461 caaaacaatt catcagcgac atactccaac ttgagggcag cgactctaaa tccattgaaa
     2521 aaagatactt aaaaaaagac ggttccttaa tctgggttat tacctcggta tctttatacc
     2581 gggataccaa tgataacccc aaatatttta ttgtccagat attggatatt actgaacaaa
     2641 aactgactga acaaaaactt atcgaagcca aaaaccaatt agaggtagtg ttcaacaatg
     2701 gccccgacgc tatcctttta acgcatattg aagatggcca ttacattgat tcaaacaaaa
     2761 cctttacgcg gtacaccggt tatacccccg acgacctcag gggcaaatcc accctcgaca
     2821 ttgacatttg gggtaatcct gacgacagaa accgaatgat taaagaagtg aaagaaaaag
     2881 ggttctgcca taattttgag ctggaaatca aaaacaaaga caggactacc agagtcattg
     2941 aaatgtcggc agaagtaatc cttatcaaag ggaagccaca cctgctgacc atttccagag
     3001 atattgccga gcgaaaaaaa atggaagaac tgcttaagaa gcaaaacgaa gagctccatg
     3061 aactggtatc caccaaagac cggtttttct ctatcattgc ccacgatttg aaagaccctc
     3121 acaatgccat tcttggcttt tcggaaatcc ttgcaggaag ttatgatatc ctatccgatc
     3181 aggacagaaa aaattatatc gtaaacattt tacattcttc acaaaatctg tcgcgactgt
     3241 tgagaaacct gctcgaatgg gccaattcgc aaaccggcag gatgatttac aaacccgaaa
     3301 gcatcaatat ctcggccgtg gtgcaggaaa ctatggacat cctgctatat caggcaaaaa
     3361 taaagcaaat caggctcata cccgatataa gctcctccca tgaggtattt gccgatccaa
     3421 acatggtgcg aaccatattg cgcaacctgg tatccaacgc catcaaatac acccacagaa
     3481 acggatttgt taaaataagc acttcctccg taacagataa aaaacacaaa acagattttc
     3541 tcgaaatttc agttatcgac aacggaatag gaataaaaaa agaaaatatt gacaaactgt
     3601 tcaaactgga tcacaaagtg aaaacccctg gcaccgaaga agaaatgggc agcggcctgg
     3661 gtcttatact ttgttatgaa ctcgtcagaa aaaacaatgg atatttgaat attagcagcg
     3721 aagaaaatgt tggtaccacc gtaacatttg ccctgccaaa aaaataacag tcaatgaaat
     3781 tcgaattttc ggacaaaaac gcgctcaccc aaatcgttga agctatcagt aatcactact
     3841 gccaaggcat ggtgatagtg gatgaagcaa gcaatacgga taaactcccg gaagaaatga
     3901 atgaccttcc tgatccggtg aaacaatttt tcagagaaaa cggaataaga atgtgccgcc
     3961 tgatgaatag ccttcccagc ccttttttca tcaaagacag caaggccaat tggttttatg
     4021 tcaacgagca tttttgcaaa ttttttaatg taaattcaaa agatattttc gggaaaaaca
     4081 gtttctgtat tttcccgaaa aaccaggccg aacaattctt tcggcaagat attgagttgt
     4141 tggaaaatgg caaagattca caggttgaaa tcgtggtcta taatgccgat ggcagcaacc
     4201 gaactatttt atcaaaaaaa actctttttc gcgacatcaa cgaccaaccg tatatcgtag
     4261 gcctgtttca tgaaataaca ggactgaaag atattgaaac atccataaaa caggaaaaaa
     4321 cagaactgga agccaaggta aaggaaagaa cttcttactg acagcaacca tcgaaaagat
     4381 gcaggaagaa attaaccagc gaaaaaaaac cgaacagg
//
LOCUS       DAOSFP010000186         4403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17926_length_4403_cov_3.958142, whole genome shotgun sequence.
ACCESSION   DAOSFP010000186 DAOSFP010000000
VERSION     DAOSFP010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17926_length_4403_cov_3.958142"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PLI16_06410"
     CDS             complement(<1..1099)
                     /locus_tag="PLI16_06410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH84229.1"
                     /translation="MMPLKKNVIGNKISLLILLLLLIFIGVERFFYYPPKSVLTWDVF
                     AYYLYLPAKFIYHDLDLMNQAWIDHIFSLYHPSDTFYQAFTGPKDVKVITVTVSLAIL
                     YAPFFLLAHGYALAFGYPADGFSMPYQYGIAIGAFIYTIIGLFFFRKVLRHFFSDRLT
                     GLLLLLTVLGTNYINQATGSNITPHNILFTLFAILVWYVIKWHQTPKIRYMVFMGLLM
                     GMITLVRPTEVVCILVPILWGIYDKQSFNEKLNLITKYKYHILLGILCFALVIFPQLL
                     YWKRQTGSFVFYSYTNPNEGLDLLSPHVGNFLLSFRKGWLIYTPLAFFYIIGLIPLYK
                     YQKNVFYAVTAFFVLSLYLIPSWSCWWYAGGC"
     gene            complement(1065..1541)
                     /gene="ybeY"
                     /locus_tag="PLI16_06415"
     CDS             complement(1065..1541)
                     /gene="ybeY"
                     /locus_tag="PLI16_06415"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOH84230.1"
                     /translation="MPVLKIDFFLQDVSFPLKNKKKIKSWLLELARAEIKNLAGVEVS
                     VVLCSDDYLLSLNKQYLNKNTLTDIITFDYCEENFLRGDIFISIDRVAENAVKFKQAT
                     CNELWRIMAHGLLHLCGYADKTPAEKTKMTEKEDFYLILLRKYMEDDASEKKCHRK"
     gene            complement(1549..2034)
                     /locus_tag="PLI16_06420"
     CDS             complement(1549..2034)
                     /locus_tag="PLI16_06420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84231.1"
                     /translation="MKKHTLIIKSDIHEITEVEKFVESICDYYNLNDTYFGNIMVALT
                     EAVENAIIHGNKSNPGKKVMIHFDFSQKGIDFTVEDQGSGYDFSAIPDATDAKANPEK
                     KGTGIYLIKTLADDVHFMDNGRKIRMIFNINSINKEVFIHRKKQLEDYFKVRKNIFEK
                     K"
     gene            complement(2034..>4403)
                     /locus_tag="PLI16_06425"
     CDS             complement(2034..>4403)
                     /locus_tag="PLI16_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84232.1"
                     /translation="KDTKKILLKFVDKSLLLEPVKNEVFLCSRNFLTSQNYTVIPQNQ
                     FIKKNDSLTYSINVIPDAYPMVDVTEYRDSVYYSHLYFKGFIKDDYGFNKLLFKYRIL
                     KQDDDNAGAELSENITFNKQINQGEFYHYFDVSALQVSPGDQIEYYFEIWDNDGVNGS
                     KAARSSKMMFKIPTENEMEKQAEQNSTDLKNKMDEAIKDISLLQKQADDINRKMLDKK
                     NLSWQERRQIEDLLIKQQQLQQNVENIKEQLHENMLSEQQFQKPDSSILEKQKQLEEL
                     FNKIMTDEMKELFRQMQEMLDKLDKTKVNEMLDKIKMNNKDLEKELDRNLELFKQLEL
                     EKKMSETIEKLQKLAEEQKELSDKTSDDKQNQQDLLDQQRRINDDFEQVKKNLQDLEK
                     LNKELEDPKKLDNTDDQQQAIQEELNNSKNSLENNKRKNASKSQKSAGDKMQSLADDL
                     LKMQQEMTQEELAEDEQTLRQLLENLIKVSFGQEDLINRLKTTNINNPKYLQIMDKQK
                     ELKDDLKMIEDSLFALSKRQSIIEPIVNKEISAINENVVKILEALHNRNISQSQKWQQ
                     YVMTSVNNLALLLAESLQQMQQQMQSMSKSCNKKGGKCSKPGNGKPSASSMKQMQEQL
                     NKQIEALKKQLESGQKPGQGGQQSMSEQLARLAAQQEALRKMLQQYGDEQKKEGAGNA
                     GQISDMMKKMEQTETDLVNKIINNETIKRQQEILTRLLESEKAEKERELDEKRKSNEA
                     IEKNYSNPNQFFEYNRLKSKELELLKTIPVTLTPFFKYKTNEYFYKFED"
BASE COUNT         1037 a          868 c          926 g         1572 t
ORIGIN      
        1 aacatccgcc ggcataccac cagcacgacc agcttggaat aagataaagg ctaagcacaa
       61 aaaatgccgt tactgcataa aacacatttt tttgatattt atatagcggt atcagcccga
      121 tgatatagaa aaaagcaagc ggagtataaa tcaaccatcc tttgcggaag ctcagcagga
      181 agttgcccac gtgcgggctc agcaaatcca gtccttcgtt gggattggta taactgtaaa
      241 acacaaaact gcccgtttgc cttttccagt ataacagttg cggaaaaata accagcgcaa
      301 aacacagtat tcccaacagg atatggtatt tatatttggt tatcaggttc agtttttcgt
      361 tgaatgattg tttatcataa ataccccaca gaatcggcac aaggatgcag acaacctcgg
      421 ttggcctcac cagcgtaatc atccccatca ggagacccat aaagaccata taccgtatct
      481 ttggcgtttg gtgccacttt attacatacc aaaccaggat ggcgaacagc gtaaacagta
      541 tgttatgcgg ggtaatattg cttccggtgg cctgattaat gtaattggtt cccagaactg
      601 tcagcaagag tagcaacccc gtgagcctgt cgctgaaaaa atgccgcaac acttttctga
      661 aaaaaaacag tcctatgatg gtataaataa aggcgccaat ggctatacca tattgataag
      721 gcatagaaaa tccgtcggct ggatacccaa aggcgagcgc gtaaccgtgt gcaagcagga
      781 aaaacggggc atacagtatg gcaagactta cggtaaccgt tatgactttt acatctttgg
      841 ggccggtaaa cgcctgatag aacgtgtcgg aaggatgata caagctgaaa atgtggtcta
      901 tccaggcctg attcatcagg tccaggtcgt ggtagataaa tttggccggc aggtacaaat
      961 aataggcaaa aacatcccag gtaagcacac tcttcggcgg atagtaaaaa aagcgttcta
     1021 cgccgataaa aataagcaat agcaataaga ttagcaggga tatcttattt ccgatgacat
     1081 tttttttcag aggcatcatc ttccatatat tttctcagca aaattaggta aaagtcctcc
     1141 ttctccgtca tttttgtttt ttcggcgggt gttttgtcgg catatccgca caaatgcagc
     1201 aggccatgag ccataatccg ccacaattcg ttgcaggtcg cctgcttgaa tttcacggca
     1261 ttttcggcca cccggtcgat actgatgaaa atatcgccgc gaaggaaatt ttcttcgcag
     1321 taatcgaatg ttattatgtc ggtaagagta tttttattaa ggtattgttt gttgagagac
     1381 agaagataat catcgctgca gagtaccacg ctgacctcta ctccggccag gttttttatt
     1441 tcggcacggg caagctccag cagccatgat ttgatttttt ttttgttctt taaagggaag
     1501 gatacgtctt gcagaaagaa atctattttt aaaacaggca ttttatgatt attttttttc
     1561 aaaaatattt ttcctgactt taaaataatc ttccaattgt ttttttctgt ggatgaatac
     1621 ttctttattg atggagttga tattaaaaat cattctgatt ttgcggccat tatccataaa
     1681 atgcacatcg tcggccaatg tttttataag gtatattccg gtaccttttt tttcgggatt
     1741 ggctttggca tcggtggcat cggggatggc ggagaaatca tagccggaac cctgatcttc
     1801 cacagtaaaa tcgatacctt tttgtgagaa atcaaaatgt atcattacct ttttgccggg
     1861 gttgcttttg tttccgtgga taatagcgtt ttcgacggcc tcggtaaggg ctaccattat
     1921 atttccgaaa tatgtatcgt tcaggttata gtaatcgcag atgctctcga cgaatttttc
     1981 tacttccgtg atttcgtgta tatcagattt tattattaag gtgtgttttt tcatcaatct
     2041 tcaaatttat aaaaatactc attggtttta tacttaaaaa acggggttaa agttacagga
     2101 attgttttta ataattcaag ttcttttgat tttaatctgt tatactcaaa aaattggttg
     2161 gggttactat aatttttttc gatggcttcg ttagattttc gtttttcgtc gagttctctt
     2221 tctttttcag ctttttctga ttcgagcagg cgtgttaaaa tttcctgttg ccgtttgatg
     2281 gtttcattat taataatttt gtttaccagg tcggtttcgg tttgttccat ttttttcatc
     2341 atgtcgctga tttgtccggc gtttcccgcc ccctcctttt tctgttcatc gccatattgc
     2401 tgaagcattt tgcgcagggc ctcctgctgt gccgccaggc gtgccagttg ctcgctcatg
     2461 gattgctgac cgccctgtcc gggtttttga ccgctttcca attgtttttt cagggcttca
     2521 atttgtttgt tgagctgttc ctgcatttgt ttcattgacg aagccgacgg tttgccattg
     2581 cccggcttgc tgcattttcc tccttttttg ttgcaggatt tcgacatgga ttgcatttgt
     2641 tgctgcattt gctgtaacga ttcggccagt aaaagggcca ggttgtttac agatgtcatc
     2701 acgtattgtt gccatttttg actttgtgag atgttacggt tgtgcagcgc ttctaatatt
     2761 tttaccacat tttcattaat ggcagagatt tccttgttaa ctataggttc gatgatgctt
     2821 tgtcttttgc tcagtgcaaa taatgagtct tcaatcattt tcaggtcgtc tttcagctct
     2881 ttctgcttat ccattatctg gaggtacttt ggattgttga tgttggttgt tttcagcctg
     2941 tttatgagat cttcctgtcc gaaagatact tttatcaggt tttcgagcaa ttggcggagt
     3001 gtctgttcat cttcggcgag ttcttcctgt gtcatttcct gctgcatttt cagcaggtcg
     3061 tcggccaggc tttgcatttt atcaccggcg cttttttgcg attttgaggc atttttcctt
     3121 ttattgtttt caaggctgtt tttactgttg ttgagttctt cctgaatagc ctgctgctgg
     3181 tcatcggtat tatcaagttt tttggggtct tccagttctt tgttcagctt ttcgaggtct
     3241 tgcagatttt tctttacctg ctcaaaatcg tcgttgattc tgcgttgctg gtcaagtaaa
     3301 tcctgttgat tttgtttgtc atcacttgtt ttgtccgaca gttctttttg ctcttctgca
     3361 agtttttgca gtttttcaat ggtttcggac atttttttct ctaattccag ttgtttaaac
     3421 aattcgaggt ttctgtccag ttctttttcg aggtctttgt tgttcatttt tattttgtcg
     3481 agcatttcgt tgacctttgt tttatcgagt ttatcgagca tttcctgcat ctgcctgaag
     3541 agctccttca tctcgtcggt cataattttg ttgaacaatt cctctaattg cttttgtttt
     3601 tcgagtatgc tgctgtcggg tttctgaaac tgttgttcgg aaagcatgtt ttcgtgcaac
     3661 tgttctttaa tattttccac gttttgctgt agctgttgct gtttgataag gaggtcttcg
     3721 atttgcctgc gttcctgcca gctgaggttt tttttatcga gcattttacg gttgatatcg
     3781 tcggcttgtt tttgcaacag gctgatatct ttaatggctt catccatttt gtttttcagg
     3841 tcggtactgt tttgctcggc ctgtttttcc atttcatttt ctgtaggtat tttaaacatc
     3901 atttttgatg accgggcggc cttgctgcca tttacgccgt cgttgtccca gatttcgaag
     3961 taatactcaa tctggtcgcc gggtgatacc tgaagcgccg acacatcaaa gtaatgataa
     4021 aattctccct ggtttatttg tttgttgaag gtaatgtttt ccgacaattc agcgcctgca
     4081 ttgtcatcat cctgttttag tatcctgtac ttgaaaagta gcttgttaaa cccataatca
     4141 tctttgataa atcctttgaa atacaaatgg ctgtagtaca cggaatcgcg atattctgtg
     4201 acatccacca tgggataggc gtcaggaata acattaattg aataggtcag cgaatcgttt
     4261 tttttgataa actggttttg cggaataacg gtatagttct gggaagtgag aaagttgcgg
     4321 ctacacaaaa acacttcgtt tttcaccggt tcgaggagca gggatttatc aacaaatttt
     4381 aacaggattt ttttggtgtc ttt
//
LOCUS       DAOSFP010000187         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17953_length_4398_cov_4.186507, whole genome shotgun sequence.
ACCESSION   DAOSFP010000187 DAOSFP010000000
VERSION     DAOSFP010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17953_length_4398_cov_4.186507"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..612
                     /gene="msrB"
                     /locus_tag="PLI16_06430"
     CDS             103..612
                     /gene="msrB"
                     /locus_tag="PLI16_06430"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOH84233.1"
                     /translation="MNLLPKVKKPILKISVLVLFALTVSCNTATHKQSDPMDTYKKIS
                     LSETEWKNKLSEEQYNVLRLKATEQPFTGKYYKLNEHGIYCCAGCGSELFSSDTKFDA
                     GCGWPSFYDALAANKIETRTDTSFQMVRTEILCAVCGGHLGHLFDDGPEPTGKRYCVN
                     SASLNFKKL"
     gene            833..1183
                     /locus_tag="PLI16_06435"
     CDS             833..1183
                     /locus_tag="PLI16_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84234.1"
                     /translation="MKTKILKRSFSLLVMLIFVISVSFASAAPRKTATDLKSVITSSV
                     TYPEYAKDHQISGFVVVAFVVDEKGNINITEMNASSLDFQLYVEKSLKKINIENPQSY
                     KGKTYYYRFDFEII"
     gene            1316..1948
                     /locus_tag="PLI16_06440"
     CDS             1316..1948
                     /locus_tag="PLI16_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84235.1"
                     /translation="MKKNILFLILMLFLSSCIEIKEVLKVNSDGSGSLTMTVDMSKAG
                     SSFSQANQQVNMTFVKEIQDAPGRADSVLKTCPGISSLKTSSEKGLYVVGFDFKNSKA
                     LNTALYKLFKQKKSIFKPDFIKVTKHKTRQMNFAPLLKKYLLKTSSTMVSDMLFQLIR
                     IESSFELPAKARNVSNIKAMQENDGQTIKMKYTLYELINNDFDYGISMTY"
     gene            complement(1950..2462)
                     /locus_tag="PLI16_06445"
     CDS             complement(1950..2462)
                     /locus_tag="PLI16_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOH84236.1"
                     /translation="MDAIENLLTRRSIRKFTGTPVNNDLIDKMLQAAMYAPSARNTRA
                     WDFVVITKRELLDRLSVIHPYARMLKMAPLAILVCGDKKLEPNEAYLSINCSAATQNI
                     LLAAHALGLGSVWLGVYSRTERMDPIRDLLKLPAENMPVSLIAIGWPDEKIPHPERYS
                     KEKIHYNDSW"
     gene            complement(2482..3261)
                     /locus_tag="PLI16_06450"
     CDS             complement(2482..3261)
                     /locus_tag="PLI16_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOH84237.1"
                     /translation="MIKKFFPLLFSFLCFISFGQSFEQTGYCTYYADRFNGQKTSSGE
                     PYNSKLFTAAHRTLPFNTVVLVTNLRNQKKVLVKINDRGPHTKGRILDVSKAAAIELD
                     IIHYGVDKVKLEVVDVPTGYLKQDTTVNLDSLIMAAKPVALKPDTRTNQITGSVEKKV
                     SPERPDDIPIIEAKGAGNFPDGYGVQMGYFKILKNCKNFLLRIQTQYDIKGYIFKETK
                     KNGVFYRLIMGDFPNKEKALQAQKKLKNEVAGCYLVNWKHL"
     gene            complement(3264..4127)
                     /locus_tag="PLI16_06455"
     CDS             complement(3264..4127)
                     /locus_tag="PLI16_06455"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOH84238.1"
                     /translation="MTKTRILIILVSFFLLSMTVDKPAYQIYNVTGKKTTYQKMIKDI
                     MSCEVVLFGELHNNPISHWMELQLTKDLFKEKKQDLILGAEMFETDMQEALDKYLKGQ
                     ITEDELTTQIKLWPNYETDYRPLVEFARNTNLKFLATNAPRKFASLVMHQGFEQLNTL
                     TDEEKKFLAPLPIEYDPELEQYKKMIEMGMAGLSSPNLPKAQALKDATMAHFIFSNAK
                     EGSLFLHYNGTYHSDYFEGIMWYLKRKNADLKILTIATVEQSDINKLSPEYIMVADYI
                     ICVPDDMTKTY"
     gene            complement(4186..>4398)
                     /locus_tag="PLI16_06460"
     CDS             complement(4186..>4398)
                     /locus_tag="PLI16_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOH84239.1"
                     /translation="ITAKSKNFDVNYPVSPCGACRQVMAEYEKKGNNPLRVILKGEKG
                     KIIIISDIAGLLPFAFDSSDLDPHQK"
BASE COUNT         1283 a          909 c          895 g         1311 t
ORIGIN      
        1 aaacaaaaga tgttttttcc ctttgccgtt gcggcaaatc aaaaaaagca ccgtattgcg
       61 atggatcaca cctgttgctt ccttttgaat aaacaacaca ttatgaattt actgcccaaa
      121 gttaaaaagc ccatactaaa aatcagcgtg ttagtcctgt tcgccctgac cgtatcgtgc
      181 aacacggcta cacataaaca aagcgacccc atggatacct acaaaaaaat atcgctgagc
      241 gaaaccgaat ggaaaaataa actcagtgag gaacagtaca atgtattacg gcttaaagcc
      301 accgagcaac cttttaccgg taagtattac aaactgaatg aacacggtat atactgctgt
      361 gccggttgcg gcagcgagtt gttttcgtct gacacaaaat ttgatgccgg ctgcggatgg
      421 ccaagttttt acgatgccct tgctgctaat aaaattgaaa cacgcaccga caccagtttt
      481 cagatggtcc gtaccgaaat actatgcgcc gtatgcggtg gtcacctggg acatttgttt
      541 gacgatggcc ccgaacccac cggtaaaaga tactgcgtaa actctgcatc attgaatttt
      601 aaaaaattat aaactgacag aaaaagaggg ttccattact ctttatctta taagctttaa
      661 ccagacctgc tgcctgaaac atccgcacac attcacagat tttacttcac ctatttattg
      721 acaggttagg ttaaagcaga aaaaattttt taaatttttt gtaacttttt ttgttctggc
      781 gacatcaaac aggcaaacga cataaattaa aaacaattaa aaaacctgcg ccatgaaaac
      841 aaaaatttta aagagatcat tttcgctgct tgtgatgctg atttttgtaa tatcagtaag
      901 ttttgcttca gctgcccccc gcaaaacagc caccgattta aaatccgtca ttacctcttc
      961 ggtaacttac ccggaatatg ctaaagacca ccaaattagc ggattcgtgg tggttgcatt
     1021 cgttgtggat gaaaagggca atatcaacat cactgaaatg aacgccagtt cacttgactt
     1081 tcagctttat gtggaaaaaa gcctgaaaaa aatcaacatt gaaaacccgc aaagctataa
     1141 aggaaaaacc tattattacc gctttgactt tgaaattatc tgataaccat atatgtcaaa
     1201 ctgtctgttt taggtttata gtttgacccc tttaaaacct gcttcttgcg ggttttattt
     1261 tttatgttct gtaacagcca atatttatat ttttgtcggg aagaaaaaac accgcatgaa
     1321 aaaaaacatt ctgttcctga tacttatgtt gtttctcagc tcctgcatcg agattaagga
     1381 agtcctcaaa gttaatagcg acgggtcggg gagcctgacc atgaccgtgg atatgtcgaa
     1441 agcaggcagt tcttttagcc aggcgaatca acaggtaaat atgacttttg tgaaagaaat
     1501 tcaggacgct cccggcaggg ccgactcagt cctgaaaacc tgccccggca tcagcagcct
     1561 gaaaaccagc agcgaaaaag gtttgtatgt agtgggtttt gattttaaaa actccaaagc
     1621 gctgaacacg gcattataca agctgttcaa acaaaaaaaa tctattttca aacccgattt
     1681 tatcaaggtt acaaaacaca aaacaaggca aatgaatttt gcacctttgt tgaaaaaata
     1741 ccttctgaaa acttcatcaa cgatggtaag cgatatgctg ttccagctaa tcaggataga
     1801 atcatctttt gaacttcccg cgaaggccag aaatgtcagc aatataaagg caatgcagga
     1861 aaatgatggc cagacaataa aaatgaaata taccctctac gaattgataa acaacgattt
     1921 tgattatggt atcagcatga cctattaggc taccagctat cgttgtaatg gattttttct
     1981 ttggaatatc tttcgggatg cggaattttt tcgtcgggcc agccaatggc aatcagcgac
     2041 accggcatat tttccgcggg cagttttaat aaatcacgta ttggatccat ccgctcggtg
     2101 cgtgagtaaa cgcccagcca tacacttccc agtcccaggg catgtgcggc aagcaagatg
     2161 ttttgggtgg ccgccgaaca attaatacta agataggctt cgttgggctc aagttttttg
     2221 tcgccgcaga ccaatattgc caggggggcc atttttaaca tacgggcata aggatggatg
     2281 acagataatc tgtcgagcaa ctcccgctta gtgataacca caaaatccca tgcccgcgta
     2341 ttccgggccg aaggggcgta cattgccgcc tgcaacattt tgtcaatcag gtcattatta
     2401 accggtgtgc ctgtaaattt ccggatactg cggcgggtaa gcaggttttc gatagcatcc
     2461 ataatttttc aattattgtg tttaaagatg tttccaatta acgagatagc acccggcaac
     2521 ttcgttttta agttttttct gggcttgcag ggctttctct ttatttggga aatcgcccat
     2581 gattaatctg taaaaaactc cgttcttttt cgtttcctta aaaatgtatc ctttgatatc
     2641 atattgggtc tgaattctca gcaggaagtt tttgcagttt ttcaatattt taaaatatcc
     2701 catctgcacg ccatatccgt caggaaagtt gccggctcct ttggcttcta taatgggaat
     2761 gtcatccggc ctttcggggg acaccttttt ctcaacactg cctgttatct ggttggtcct
     2821 ggtgtcgggc ttcagggcaa caggtttcgc agccattatc agcgagtcca ggttgacggt
     2881 agtatcctgt ttgaggtatc cggtcggcac atccaccact tcaagtttta ccttgtcaac
     2941 accataatga ataatatcca gttcgatggc ggcagcttta gaaacatcca gaatcctacc
     3001 tttggtatga gggcccctgt cgttgatttt taccagcact tttttttggt ttcggagatt
     3061 ggtaacgagc actacggtgt taaacggcag ggtacgatga gctgcagtaa acaatttgct
     3121 attatacggt tcgccgctag atgtcttttg cccgttaaac ctatcagcat aataagtgca
     3181 gtaaccggtt tgttcaaacg attgcccgaa acttataaaa cagagaaaag aaaaaagaag
     3241 cggaaaaaat tttttaatca atattaatag gtttttgtca tatcatccgg gacacaaata
     3301 atataatcgg caaccataat atattcagga gaaagtttgt taatgtcgct ttgttccact
     3361 gtggcaatgg taagtatttt taagtctgca ttttttcgtt tcaggtacca cattattcct
     3421 tcgaaataat cggagtggta ggtgccatta taatgcagga acaaactgcc ttctttagcg
     3481 ttgctaaaaa taaagtgcgc catagtagca tctttcagag cctgagcctt gggcagattg
     3541 ggagacgaaa gccccgccat acccatttca atcattttct tatattgttc cagttcgggg
     3601 tcatactcga tgggtaaagg cgccaggaac tttttctcct catcggtaag ggtatttagt
     3661 tgttcaaatc cttggtgcat caccagggat gcaaatttgc ggggagcgtt ggtggctaaa
     3721 aatttcaggt tcgtgtttcg tgcaaattcc accagggggc gatagtcggt ttcgtagttg
     3781 ggccagagtt ttatttgtgt tgtcagttcg tcttcggtaa tttgcccttt cagatacttg
     3841 tctaaagctt cctgcatatc agtttcaaac atttcggcac ccaggatcag gtcttgtttt
     3901 ttttctttaa aaagatcttt tgtaagctgc aattccatcc agtgcgaaat aggattgtta
     3961 tgtagttcac caaaaagcac cacttcgcaa ctcatgatat ctttgatcat tttctgatag
     4021 gtagtttttt tcccggtaac attatatatc tgataggccg gtttatcaac ggtcatcgag
     4081 agcagaaaaa aactgacaag aataattaga atacgggttt ttgtcataac aaattactat
     4141 ttatgagaaa acaaatatat aaaatttaaa ataaaaaccg attgattatt tttgatgcgg
     4201 gtccaggtcc gaagaatcaa aagcaaaggg cagcagcccg gcgatatcag aaattattat
     4261 aatttttcct ttttctcctt tgaggattac ccggagtggg ttatttcctt ttttttcgta
     4321 ttcggccatt acctgtctgc atgcgccaca gggcgaaacg ggatagttta catcaaagtt
     4381 ttttgatttt gccgtaat
//
LOCUS       DAOSFP010000188         4392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_17989_length_4392_cov_4.545538, whole genome shotgun sequence.
ACCESSION   DAOSFP010000188 DAOSFP010000000
VERSION     DAOSFP010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17989_length_4392_cov_4.545538"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /locus_tag="PLI16_06465"
     CDS             <1..1988
                     /locus_tag="PLI16_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84240.1"
                     /translation="TVSFSTRQGKFGFVEGTSYDAYSYFLSVEKKIGKKHYLSFTGFG
                     APAKRGMQSAAVQEVYDLLNDNYYNPNWGWQNGEVRNSRIRTTHEPVILLSHYFDIND
                     KMKLTTTIGTSFGRMGTTALNWYDAADPRPDYYRYLPSYQENKDLDEAADPMVVQALT
                     EAWQNDESFSQINWNRLYQTNYLSYLNGGQAKYILEERRLDHVQGGLSSHLNYEINNH
                     IFFTGGLNVNLYKSHNYKVIADMLDGMTDNNTGEAYEGWWVDIDQYSERDFPSDTLIL
                     QNDLNNPDRIVRKGDTYGYDYNIHMNKANLWGVAEFTYTHFDFYAGLNLTGSQYWRVG
                     NMKNGRFADDSYGKSEKHNFFDYAVKAGVTYKPTGRHYILLNGGYMTMAPSFSDVYLS
                     PRVRNTVIPDIKSKKIFSADLSYIVRYPKFNGRVTAFHTMFWNDTKVNGFYHDDLRTF
                     VNYMMFDINKVHQGIELGAEVKIIPVLSATLVASYGNYRYTNRPTGYMSVENGSLPDS
                     MQTIYCKNFYVNGTPQAAGSLGLKFNHNFWFANLNANLFANNWVDFSAARRTVEAIQY
                     LYPGDARINSITEQYKANEKPQFTLDASVSKSWKIKKFYLILNGSVSNILNNTKMITS
                     GYEQLRFDYNTLDTNKFPAKYYYGLGRTFYFMITFRY"
     gene            2018..2794
                     /locus_tag="PLI16_06470"
     CDS             2018..2794
                     /locus_tag="PLI16_06470"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOH84241.1"
                     /translation="MKIFKMTSKMWLMTVAATLVVMTSCKKELDTPPLAEPVFTLPAG
                     ATLMTIADFKTTPAGTVTDDVYITGTVCANDISGNIYKYLYFQDTTGGIGIGLDKICL
                     YNIYSVGQKIYVKCNGLDFRFVNSMPYLGIPYNGAQTAIPEDDVDKYVFRHGLPGTAP
                     DALVHATSGLTDNLLGMLVRFDSVSFADAGETFNSDVYQSTQNRIISDGTGDVTLRIS
                     GYANFHADTIPSGKGTVYGILTKYNSTWQLMLRDINDVVF"
     gene            2847..4136
                     /locus_tag="PLI16_06475"
     CDS             2847..4136
                     /locus_tag="PLI16_06475"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOH84242.1"
                     /translation="MKTIKTKKIWILALTALVFSMTGCKKDFDVPQPEVPAFTLPAGA
                     TLKTIADFKITTTGIVTDDVYICGIVGGNDISGNIYKFLYIQDSTGGLGIEIDKVNMF
                     NIYPVGQRVYVKCQGLHFGFEKGMPNLGGFYNGAVIPIPEVFVNDRIFRDGLPGPAPA
                     AMLHTTTNLTDDHLGMLVRFDSVSFNAAGQIFNDDDAYKETFNRIITDGAGDVTARIS
                     WYADFHRETIPSGKGTVYGILTKYNSTWQLLIRDYNDLQGFDFATKAIFTEPFESGMG
                     DFTTYDSLGTQAWTFSSSYGMTITGYESGTRYANKDYLISPALDLTQYDSVYLAFDQA
                     INYYSGSNIQSRHSVWITTNYNASNPAATTWVSLSVPTYPAGNSWSFVNSGKAYIPAA
                     FNGATNVRFAFKYVSTGTSSETSTWEIKNVVLMGKQN"
BASE COUNT         1284 a          957 c          933 g         1218 t
ORIGIN      
        1 ccacggtgtc tttttctacc cgtcagggaa aatttggctt tgtggaagga acctcctatg
       61 atgcctattc ctattttctt tctgtggaaa aaaagattgg gaaaaaacac tacctgtctt
      121 ttacaggttt cggagccccc gccaaaagag gcatgcagtc ggctgccgtt caggaagtgt
      181 atgatttgtt aaacgataat tattataacc ccaactgggg atggcaaaac ggcgaggtga
      241 gaaattccag gataagaact acccacgaac cggtgatttt gctgtcgcat tactttgata
      301 tcaatgataa gatgaaactt accacaacca ttggtaccag ctttggacgt atgggaacta
      361 cggcgcttaa ctggtatgat gccgctgatc cccgccctga ttactacaga tacctgccaa
      421 gctaccagga aaacaaagac ctcgacgaag ctgccgatcc catggtggtt caagccctta
      481 ccgaagcatg gcaaaatgat gaaagcttca gccagatcaa ctggaacagg ctttatcaga
      541 ccaactacct cagctacctg aatggcggcc aggctaaata catcctcgaa gaacgccgtc
      601 tcgaccatgt gcagggcggt ttatcaagcc atcttaacta cgagataaac aatcacattt
      661 tctttactgg cgggctgaat gtcaacttat acaaaagcca taactacaag gtgattgccg
      721 atatgcttga tggtatgact gataataata ccggtgaagc ttacgaaggc tggtgggttg
      781 atattgacca gtattctgaa cgcgattttc cttcagacac cctgattctg cagaatgacc
      841 tgaacaatcc cgacagaata gtcagaaaag gagataccta tggttatgac tacaatatcc
      901 acatgaataa agcaaatctg tggggcgtcg ccgaatttac ctatacccat tttgattttt
      961 atgccggact gaaccttacc ggtagccagt actggagagt aggtaatatg aaaaacggcc
     1021 gttttgccga cgattcttat ggcaaatccg aaaagcacaa tttctttgat tatgcagtaa
     1081 aagccggtgt aacctacaag cccaccggac gtcattatat tttgctgaac ggtggctaca
     1141 tgaccatggc gccttctttc agcgatgtat acctctcgcc cagggtgaga aataccgtca
     1201 tacccgatat caaatccaaa aagattttca gcgctgacct gagttatatc gttcgctatc
     1261 caaaattcaa cggacgtgta actgctttcc acactatgtt ctggaacgat accaaggtaa
     1321 acggttttta tcacgacgac ctcaggacct ttgtgaatta tatgatgttt gatatcaaca
     1381 aagtacacca gggaatagaa cttggtgcag aagtcaagat tattccggta ttatccgcaa
     1441 cgctggtggc ttcttacgga aactacagat acaccaacag gccaacaggt tatatgtcgg
     1501 tcgaaaatgg ttccttacct gattccatgc agacgatata ttgtaaaaac ttctatgtaa
     1561 atggtacgcc tcaggccgcc ggttcgcttg gactgaagtt taatcataat ttctggtttg
     1621 ccaacttaaa tgccaacctt tttgccaaca actgggttga tttcagcgca gcccgccgta
     1681 ccgttgaagc catacagtat ctgtatccgg gcgatgccag aatcaatagc attaccgaac
     1741 agtacaaagc aaacgaaaaa ccgcaattca ccctggatgc ttcagttagc aaatcctgga
     1801 aaatcaaaaa attctacctg attttgaacg gaagcgtgag caacattttg aataatacca
     1861 aaatgattac ctcagggtac gaacaactca ggtttgatta caatacgctg gataccaata
     1921 aattcccggc aaagtattat tatggactgg gccggacttt ctatttcatg attactttca
     1981 gatactaatt acaaattgaa attcaaaaat taaggatatg aaaattttca aaatgacaag
     2041 caaaatgtgg ttaatgaccg tagcagctac tctggtcgtt atgacaagtt gcaaaaagga
     2101 attggataca ccaccccttg ccgagccagt gtttacgttg cctgctggag ctacactgat
     2161 gactattgct gattttaaaa ccaccccggc agggactgtg acagatgatg tttatattac
     2221 cggcacggta tgcgctaacg atatttcggg aaatatatac aaatacctgt attttcagga
     2281 tactaccgga ggcattggca ttggattgga taagatttgc ctttacaaca tttattcggt
     2341 agggcagaag atttatgtga aatgtaatgg actggatttc cgttttgtaa atagcatgcc
     2401 ttacctgggt attccctata acggagctca gacagccatt cctgaagatg atgtggacaa
     2461 atatgtattc cgtcacggat taccgggtac agcgcctgat gcactggtgc atgccacttc
     2521 cggactgaca gataatctgc tcggtatgct ggtacgtttc gacagcgtat catttgccga
     2581 tgccggagaa accttcaaca gcgatgtgta ccagagtact caaaaccgca tcatttctga
     2641 tggaacggga gatgtaaccc tgcgcatcag cggctacgct aattttcatg ccgacaccat
     2701 tccatccgga aaaggaacgg tttatggaat actgacaaag tataattcta cctggcaact
     2761 aatgttgcgc gatatcaatg atgtagtgtt ttagttgtcg agttgctttt tgaactataa
     2821 tttagcttat aaagaaaata aataaaatga aaacgataaa aacaaaaaaa atctggatat
     2881 tggcactcac tgcccttgtg ttttcaatga caggctgcaa aaaggatttt gatgtgcctc
     2941 agcccgaagt ccctgcgttt actttgcctg ccggtgctac actaaagacc atagctgatt
     3001 ttaaaatcac taccacggga attgttaccg atgatgtata tatctgtggc atagtgggtg
     3061 gcaacgatat ttccggaaat atatacaagt ttctttatat tcaggactct acgggaggat
     3121 tgggtataga gattgataaa gtaaatatgt ttaacattta tcccgtagga cagcgcgtgt
     3181 atgtgaaatg ccagggcctg cactttggtt tcgaaaaagg catgcccaac cttggaggat
     3241 tttacaatgg agcagtgatt cctattcctg aggttttcgt taacgaccgt atcttccgcg
     3301 atggactgcc cgggcctgca cccgcagcca tgctgcatac caccaccaac ctgacggacg
     3361 atcatcttgg catgctggtg cgtttcgaca gcgtttcgtt caatgccgcc ggacagattt
     3421 ttaatgacga tgatgcgtat aaagaaacat ttaaccgtat cattactgat ggtgccggcg
     3481 atgttaccgc aaggattagc tggtacgctg attttcaccg ggaaaccatt ccttcaggaa
     3541 aaggcaccgt ctatggcata ctcacaaaat ataacagcac ctggcaactg ttaatcagag
     3601 attataatga tttgcagggt tttgattttg ccacaaaggc tatttttacc gaacctttcg
     3661 aatcgggaat gggcgatttt accacttatg actctttagg tacacaggca tggaccttca
     3721 gctccagcta cggtatgacg atcacaggct acgaaagcgg tacccgctat gccaataaag
     3781 attatttaat ctccccggct ttagatctta cacaatacga cagcgtttac ctggcttttg
     3841 accaggctat caattactat tccggatcaa atattcagag tcgtcattcc gtgtggatca
     3901 ctactaatta taacgcatct aatcctgcag ccaccacttg ggtaagtttg tcagtgccta
     3961 cgtatcctgc aggtaatagc tggtcctttg tcaattcggg caaagcatac atacctgcgg
     4021 ctttcaacgg tgctaccaac gtgcgttttg cctttaaata tgtcagcacg ggtacatcca
     4081 gcgaaaccag tacctgggaa ataaagaatg tggtccttat gggcaaacaa aattagtaaa
     4141 acaccttttt gtttaaaggg ttgtttccgc gaggaacaac ccttttttat ttgtttacta
     4201 gtcgatatta ggcatagaaa aatgtaaaga atattttgtc cgtaggccat aaacattaat
     4261 actctttaat ctactgcaca gaatatattt tttttgaatt tctttttcta ttgatgcctg
     4321 gcctgaaaaa ttcattcgaa tttttactct tcgggccgac aaaagaaaat ccgttaatcc
     4381 ggtaatgatt tg
//
LOCUS       DAOSFP010000189         4388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18012_length_4388_cov_4.720286, whole genome shotgun sequence.
ACCESSION   DAOSFP010000189 DAOSFP010000000
VERSION     DAOSFP010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18012_length_4388_cov_4.720286"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLI16_06480"
     CDS             <1..1143
                     /locus_tag="PLI16_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOH84243.1"
                     /translation="FFLGQNNIYSIAPFDPIDFSNLVFFAVLGIFCGFISVYFTRVLK
                     FFDKHFNKIKNTYIRLFVGGGILGGLILLLPSLYGEGYHQINQCLRGNYDFVFGNNPF
                     GFLGNGVGAAIILLAAIIIFKVIATSLTFSAGGVGGIFGPTLFLGAVTGLLFALLVGF
                     LDIKELPATKFALAGMTGLLAGVMHAPLTGIFLIAEITGGYQMIIPLIITASISYLTI
                     RSFIPYSVDAIELAEKGELITHDKDKSVLNLMKIDKLIETNFITVSPDDYLGQLVKVI
                     ARSERNIFPVVDKENNLLGIIFLNDIRGVIFNQALYGKLFVHNLMFMPSPLVDPDESM
                     EDVAMKFEKTDNYNLPVVKDGKYLGFVSRANFFSQYRQMVKQFSED"
     gene            1145..1588
                     /gene="umuD"
                     /locus_tag="PLI16_06485"
     CDS             1145..1588
                     /gene="umuD"
                     /locus_tag="PLI16_06485"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOH84244.1"
                     /translation="MKKKSKNAFEVFVPATSEKLPLPLYVAKISAGFPSPAEDYIERK
                     LDMNEYLIKNPSATFFVKVSGQSMINAGIHDGDILVVDRSLDAADNRIVIAVVNGEFT
                     VKRLMKKNNKLFLVPENENFNPIEINDTMDASIWGVVTYTIHKLR"
     gene            complement(1591..2100)
                     /locus_tag="PLI16_06490"
     CDS             complement(1591..2100)
                     /locus_tag="PLI16_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84245.1"
                     /translation="MKARFTIIILLATAIAAFVACNKEEDFPEVPYLEYRDFTVLRNS
                     NNIDEKGILKIYFTDGDGDMGLDNGDTLPPFDKNSIYYYNFFITYYEKQHGQFVKVEP
                     SVTFNSRIPVLESSSSNGAIKGEIEIEMFINNPFSVYDTIKFEAYIVDRALQASNLIS
                     TPDIILKKH"
     gene            2153..3133
                     /locus_tag="PLI16_06495"
     CDS             2153..3133
                     /locus_tag="PLI16_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOH84246.1"
                     /translation="MNSEELKFKIFNITSFADFNDLALQIFHFQYKQNAVYQKFVDLL
                     PIAVDTITSCYDIPCLPVEFFKSHEIRSVDTPAELVFMSSGTTSAQRGKNLVFSQELY
                     EKSIAASFSSFVGNPEECIVLALAPDYQKEPQSSLAFMLDFLIKKSGHRESGFYLNNT
                     AGLKEVLTASPGKKKILFGISWALLDFAEQNPCKLNDTLVIETGGMKGRRKEITLYEL
                     HEVLKKNLGVAEVFSEYSMCELMSQSYSTGRGLFKTPPWMKIYIREFNDPFARAGNEK
                     NGGINIIDLANLYTCSFISTKDVGVMHADGTFEVHGRYDDSDIRGCSQMV"
     gene            3233..3433
                     /locus_tag="PLI16_06500"
     CDS             3233..3433
                     /locus_tag="PLI16_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84247.1"
                     /translation="MTVLTVIAFASCKKCVTCSYEYDSLGQKQVVTYPQECGSSKKIN
                     DYKDMVEADALRHGVESTCTKD"
     gene            complement(3527..4195)
                     /locus_tag="PLI16_06505"
     CDS             complement(3527..4195)
                     /locus_tag="PLI16_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOH84248.1"
                     /translation="MCGRFSFAISEKIIEEHFGFVPENYQARYNCAPSQTLAVISNQD
                     TGRFSYFKWGFVPFWAKDLSVGSKMINARAETVSSKPAFRQALQARRCLVPADSFYEW
                     SHDAGKTPFRIMLNGTPLFTMAGIWSEWTTPQQEIITTFAILTIESNTLVRQLHQRMP
                     VILDTKEAENTWLKSDSEAELQQLCVPIAAKKMSIYPVSIKVNSPKNDGPGLHQQVNQ
                     TKLF"
BASE COUNT         1224 a          898 c          968 g         1298 t
ORIGIN      
        1 ttttttctgg gacaaaacaa tatttatagc attgctcctt ttgatcctat cgatttttcc
       61 aacctggtgt tttttgctgt tctgggaatt ttttgcgggt ttatctcagt gtattttacc
      121 cgtgtattga agttctttga taaacatttc aataaaataa aaaacacata tatcagactg
      181 tttgttggag ggggtatact tggcggcctg attttattat tgccgtcact ctatggtgaa
      241 ggatatcacc agataaacca atgtctgaga ggaaattatg attttgtgtt cggaaacaat
      301 ccttttggct ttttgggcaa cggcgtaggt gcggccatta tcttactcgc tgccatcatc
      361 atttttaaag tgattgccac ctcgctcaca ttcagcgccg gaggtgtggg cggtattttt
      421 ggccccacgc tttttttggg tgccgtaaca ggacttttgt ttgccctgct ggttggtttt
      481 ctggatatca aagagttgcc cgccaccaaa tttgccctgg ccggcatgac cggcttgctg
      541 gcaggagtaa tgcacgcgcc actgaccggc atatttctta ttgccgagat taccggaggc
      601 tatcagatga taattccgct gattatcacc gcttccatct cgtatctgac gataaggtcc
      661 tttatcccct attcggtgga cgccatagaa cttgccgaaa aaggcgaact gataacacat
      721 gataaagaca aatcggtgct gaacctgatg aaaattgaca aactcattga aaccaatttc
      781 attacagtgt cacccgatga ttatcttggg caactggtaa aggtaattgc gcgttccgaa
      841 cgtaatattt ttccggtggt tgacaaagag aacaatctgc tgggcatcat ctttcttaat
      901 gatatccggg gtgttatttt taatcaggcc ctgtacggta aattatttgt ccataatctg
      961 atgtttatgc cttcgcccct ggtggatccc gacgaatcga tggaggatgt ggctatgaaa
     1021 tttgaaaaaa cggataacta taaccttccc gtagtaaaag acggaaaata tttaggattt
     1081 gtgtcgcggg caaatttctt ttcgcagtac cggcaaatgg ttaaacaatt ttcagaggac
     1141 tgaaatgaaa aaaaagtcaa aaaacgcttt tgaggttttt gtaccggcaa catcggaaaa
     1201 gcttccgctg ccgctttatg tggctaaaat ttcggcgggt tttccctctc ctgccgaaga
     1261 ttatattgaa cgcaaactcg acatgaacga atacctgata aaaaaccctt cggcgacttt
     1321 ttttgtaaaa gtgtcggggc aatccatgat caacgcgggt atccacgatg gcgatatttt
     1381 ggtggtggac cgttcgctgg atgccgccga caacaggata gtgatagcgg tagttaacgg
     1441 ggagtttacc gtaaaacgac tgatgaaaaa aaacaacaaa ttgtttcttg tacctgaaaa
     1501 tgaaaacttt aacccgatag agattaatga caccatggat gcatccatct ggggcgtggt
     1561 cacctacacc attcacaaac tgcggtaggt tcagtgtttt ttcaggataa tatccggggt
     1621 ggaaatgagg ttgcttgcct gcaacgccct gtcaacaatg tatgcctcaa atttaatagt
     1681 atcatagacc gaaaaaggat tgttgataaa catctctatc tcaatctctc ccttgatagc
     1741 gccattgctg cttgaggatt ccaacacggg aattctggaa ttgaaagtta cggaaggttc
     1801 taccttcaca aattgcccat gttgcttttc gtagtaggta ataaaaaaat tataataata
     1861 aatgctgttt ttatcaaaag gcggcagggt gtcgccgttg tcaaggccca tgtcgccgtc
     1921 gccgtcggta aaataaattt tcagtatgcc tttttcgtca atattatttg aattccgcag
     1981 cacggtaaaa tcccggtatt caaggtaggg cacctcggga aaatcctcct ctttgttgca
     2041 ggcaacaaat gccgcaatcg ctgtagccag caggattatt attgtgaaac gggctttcat
     2101 ccggatattc ttagtaattt tgaatataaa attacacaaa attatcgatg taatgaatag
     2161 tgaagaactg aaatttaaaa tatttaatat tacctcattc gcggatttta acgatcttgc
     2221 attacaaata tttcattttc agtacaaaca aaacgctgtt tatcaaaagt ttgtagatct
     2281 gttacccata gctgttgata ccattacttc gtgttatgat attccctgcc ttccagtgga
     2341 gttttttaaa tcgcacgaga ttcgaagtgt ggacactcct gcggaacttg tctttatgag
     2401 tagcgggacg acctcggccc aacggggaaa aaacctggtg ttttcacagg aactatatga
     2461 aaaaagtata gccgctagtt tttcatcctt tgtaggaaat cccgaagaat gtattgtact
     2521 tgccctggcg cccgattatc aaaaggaacc gcaatcatcg ctggctttta tgctggactt
     2581 tcttatcaaa aaatccggac accgcgaaag tgggttttac ctgaacaata cggccggatt
     2641 aaaagaagta ttgaccgctt ctccgggaaa gaaaaaaata cttttcggca tcagctgggc
     2701 gctgctcgac tttgcggaac aaaatccctg taaactgaac gatacgctcg ttattgaaac
     2761 cggtggcatg aaaggccgca gaaaagaaat taccctctac gaactgcatg aggttttgaa
     2821 aaaaaatctg ggtgtcgctg aagtgttttc ggaatacagc atgtgtgagt tgatgtcgca
     2881 gagctattcg actggcaggg gactatttaa aacacctccc tggatgaaaa tttatatccg
     2941 tgaatttaac gacccttttg cccgggccgg aaatgaaaaa aacggcggaa taaacatcat
     3001 tgacctggcc aacctttata cctgttcttt tatttctacc aaagatgtag gggtaatgca
     3061 cgccgacggt acttttgagg tgcatggacg gtacgacgac agcgacatca gggggtgcag
     3121 tcagatggtg taattatatt ttttaaaata aaggctattt ccaaattagt ttatatattt
     3181 gcaaaggaaa atttaatacc gaagatccat gagaaaaatg tttttattag ctgtgactgt
     3241 acttactgta attgcttttg cctcctgcaa aaaatgtgtt acctgtagtt atgaatacga
     3301 ttctcttgga caaaaacagg tagttactta cccacaggaa tgcggcagta gcaagaaaat
     3361 caacgattat aaagacatgg ttgaagccga tgccttacgt catggtgtag aatcgacctg
     3421 cactaaagac tgatattttt ctgtccactg ttgcctttgc gggcaataat aatactcttc
     3481 ggcagcaata attgataaca attattgctg ttttattttt ccttgctcaa aaaagctttg
     3541 tctggtttac ctgttgatgc aggccggggc catcattttt cggggagttc acttttatcg
     3601 aaacaggata tatcgacatt tttttagcag ctatgggcac acacagttgt tgcagttcgg
     3661 cctcagagtc actctttaac caggtatttt cggcttcttt ggtatcaagg atcaccggca
     3721 tgcgttggtg tagctgccgt accagtgtgt ttgattcgat ggtgaggatg gcaaatgtcg
     3781 taataatttc ttgttggggt gttgtccatt cgctccagat gccggccatg gtaaacaagg
     3841 gtgttccgtt aagcataatt cgaaaagggg tttttccggc atcatgcgac cattcgtaaa
     3901 atgaatcagc gggtacgagg caccgccggg cctgcaacgc ttgtctgaac gctggtttcg
     3961 aggatacggt ttcggcgcgg gcattaatca ttttgcttcc caccgataag tcttttgccc
     4021 aaaaaggtac aaaaccccat ttaaaataac taaaacgacc tgtatcctga ttgcttataa
     4081 ctgccagggt ttgtgaagga gcacaattat agcgggcctg gtagttttca ggaacaaaac
     4141 caaaatgttc ttcaataatt ttttcgctga tagcaaaaga aaaccgcccg cacatagtta
     4201 cgcggtttaa aaaattattt tatcagcacc acattgccct tgagggcatg atggtgcttc
     4261 aaggcatcat aataggacac ataccacacg tacaaacctt cgttggcaac actgccgtta
     4321 acggttccat cccagccggt ttcgatgtca gtggtggtaa acaccaattt tccccagcga
     4381 tcgtagat
//
LOCUS       DAOSFP010000190         4379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18071_length_4379_cov_3.208372, whole genome shotgun sequence.
ACCESSION   DAOSFP010000190 DAOSFP010000000
VERSION     DAOSFP010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18071_length_4379_cov_3.208372"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="PLI16_06510"
     CDS             <1..163
                     /locus_tag="PLI16_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HOH84249.1"
                     /translation="VIDKVLESEKHDYLYFCAKEDLSGYHNFAETAAQHAQNARRYHA
                     ALNRMKIKK"
     gene            214..1041
                     /locus_tag="PLI16_06515"
     CDS             214..1041
                     /locus_tag="PLI16_06515"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH84250.1"
                     /translation="MKRLLFATLIALFATSAFALNPKREYIARPGDFGLDYEEVTIQT
                     SDNLNLKGWLYKPAQPSFKMIILSGSGDGNMGDLIEIASSFVTLGYNVLTYDYRGYGE
                     SEDFKINNNFYIYAQFEKDLNAAIDYARKYHSKMKTLNLWGKEVGAGLSIAVGCNRKE
                     IFQVIADSPYNTLEDIKKKFKDVGGKDVLLPLGFNKYMIEPFYALESKGTSLYGVMFI
                     AGENETIYSADDIKNLSKVQKNRSTVFIVKGANASETFTKDKNKYFENIRGFLKDPL"
     gene            1053..2396
                     /locus_tag="PLI16_06520"
     CDS             1053..2396
                     /locus_tag="PLI16_06520"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOH84251.1"
                     /translation="MKAFKAYDIRGVYDVDFTKDDVYKIGFFLPDLLKTGKILVGRDV
                     RTSSPEIFEYLTKGITDAGADVYDLGLATTPYVYWATAAFNFDASVQITASHNSKEYN
                     GMKISRTNAFPVGYDSGLNILEQQVMNGTPVPVDKKGKLIPFKQHEKYLEFFKPYIPD
                     ISNLRVSIDCSNGMASLFIKQLLGVSPMYMFFEPDGTFPNHEANPLVPENVAFLKEHV
                     LKHKSDLGIIFDGDADRVMFVDEQGQFISPDLIIAVLAHYFADKEKGVVLQDIRSSKA
                     VGEYIARFGYTLSMWRVGRAYAAPKLKEIKGLYGGEFAGHYYFRDFNYSDSGILACLL
                     VLDVVAAMKSKGVSVSQLIKDISSYYGSGEINYKIQQKQEAMDALLAHFTHSEKPVAF
                     YDFDGYRADFEHWWFNVRPSNTEPYLRFLAEADDPALLERVLEETNSILKPFINQ"
     gene            2400..3443
                     /locus_tag="PLI16_06525"
     CDS             2400..3443
                     /locus_tag="PLI16_06525"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOH84252.1"
                     /translation="MPKNKISAALVLFCLLWFTPAIFAQSDSVAIKTKNIFPESKSNR
                     QFADSMKSIYRQIEKVNMDCRLAVFPKELNKKRLIPLACAEGVLTGSSLYALNEMWYK
                     NYDRSAFHFFNDSREWMQMDKLGHLKASVTISNFCYFAFRWSGVNKSKSVIYGSALGL
                     GYMTAIEVLDGYSSHWGFSVTDMAVNVLGTAIFASQEMLWENRRVKFKISYHPTQYAG
                     YRPEALGDNIAERMLKDYNGQTHWLSISSGLLMPHQKKVPRWLCVSFGYGAEGMTGGF
                     ANPAFNEKGDALPAFDRYRQFYLSFDVDFAQIKTRSKFVRGLLLALNTLKVPFPTLEY
                     NTKGEFKFHYLYF"
     gene            3889..4317
                     /locus_tag="PLI16_06530"
     CDS             3889..4317
                     /locus_tag="PLI16_06530"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOH84253.1"
                     /translation="MTFETASLLSRGFKNITTNKFDADLGHKLENVVYFELLRHGGMV
                     FVGKNKDKEVDFILQKANNERGYFQVAYSVNDEKTLEREISSFRSIKDNYPQYLLTLD
                     YDNAVIDGIQKKKIFVLSVHRFFCRQLYHNCIVNDMSMHF"
BASE COUNT         1256 a          891 c         1021 g         1211 t
ORIGIN      
        1 tgtgattgac aaagtattag aaagcgaaaa gcatgattac ctgtattttt gtgccaaaga
       61 agatttgtcg ggctaccaca attttgccga aaccgccgcg caacatgctc aaaacgcccg
      121 caggtaccac gccgcactca atcgtatgaa gatcaaaaaa tgatgactgt gaatgaaaat
      181 tttttaattt taccaaccaa atagtaaatg atcatgaaaa gactgctttt tgcaaccctg
      241 attgccttgt ttgcaacttc ggcatttgcc ctgaacccca aacgcgaata catcgcccgt
      301 cccggcgatt tcgggctgga ctatgaagag gttactatcc agacttccga caacctgaac
      361 ctgaaagggt ggctgtacaa acctgcacaa ccgtcgttca aaatgattat cctcagcggc
      421 agcggcgacg gcaatatggg tgatcttatt gagatagcca gcagttttgt taccctgggt
      481 tacaacgtgc tgacatacga ctataggggt tatggcgaaa gcgaagattt taagatcaac
      541 aacaattttt acatttacgc ccagtttgag aaagacctga atgctgccat cgactatgca
      601 agaaaatacc attcgaagat gaaaaccctc aacctctggg gtaaagaggt aggagcgggg
      661 ctttcgatag ctgtagggtg caaccggaaa gagatttttc aggtcattgc cgattcgcct
      721 tataatacgc tggaagacat taaaaagaaa tttaaagacg tgggtggcaa agacgtgttg
      781 ttgccgctgg ggtttaataa atacatgatt gagccgtttt atgcccttga atcgaaaggt
      841 acttcgctct acggcgtgat gtttattgcc ggagaaaacg aaaccattta ttccgctgac
      901 gatattaaaa atctcagcaa agtgcagaaa aaccgctcta ccgtatttat tgtcaaaggc
      961 gccaatgcgt cagagacctt taccaaagat aaaaacaaat atttcgagaa tatccgtgga
     1021 tttctcaaag atccattata aaatttatac agatgaaagc atttaaagcg tatgatatcc
     1081 gtggggtata tgatgttgat tttacaaaag atgatgttta taagattggt ttttttctcc
     1141 cagacctgtt aaaaaccggg aaaatactgg ttggccgcga tgtgaggact tcctcgcccg
     1201 agatttttga atacctgacc aagggcatta ccgatgcggg agccgatgtg tatgacctcg
     1261 gcctggccac cacgccctat gtatattggg ctacggcagc atttaatttt gatgcttcgg
     1321 tgcagatcac tgcctcgcac aattcaaagg aatacaacgg catgaaaata tcgcgtacca
     1381 acgcctttcc ggtgggttac gacagcgggc ttaacatcct ggaacaacag gtgatgaacg
     1441 gaactccggt acctgtcgat aaaaaaggaa agctgatacc tttcaaacaa catgaaaaat
     1501 atttggaatt tttcaagcct tatatccccg atatttcaaa cctgagggtg agcatcgact
     1561 gttccaatgg catggcttcg ttgtttatca agcagttgct gggtgtgagt cccatgtata
     1621 tgttttttga gcccgatgga acttttccca accatgaagc caacccgctg gtaccggaaa
     1681 atgtggcctt cctgaaagaa catgtgctga aacataagag cgacctgggc atcatctttg
     1741 atggcgatgc cgaccgtgtg atgtttgttg atgaacaggg gcagtttatc tctcccgacc
     1801 ttatcattgc tgtacttgct cattactttg ccgataagga aaaaggggtg gtgctgcagg
     1861 atatccgcag ctcgaaagct gtgggtgaat acattgcccg ttttggttac acgttgagca
     1921 tgtggcgcgt gggaagagct tatgccgctc ccaagctcaa ggaaataaaa ggcctctacg
     1981 gtggcgagtt tgccgggcat tattacttcc gcgacttcaa ttattcagat tccggtatcc
     2041 tggcctgcct gctggtgctt gacgtggtgg cggccatgaa atcaaaaggc gtcagtgttt
     2101 cgcaactgat aaaagacata tcttcatact acggctcggg cgagataaat tataagattc
     2161 agcaaaaaca ggaagctatg gatgcgttgc tggcacattt cacccatagt gaaaaaccag
     2221 ttgcctttta tgattttgac gggtaccgtg ccgattttga gcattggtgg tttaatgtaa
     2281 ggccctcgaa tacggagccc tatcttcgtt ttctggccga agccgatgat ccggcactgc
     2341 tggaacgtgt tcttgaagaa acaaacagca tactcaaacc attcataaat cagtgatttt
     2401 tgccaaagaa taagatttcg gcagccctgg tattattttg ccttctgtgg tttacaccgg
     2461 ccatatttgc gcagtcggac agtgtggcta ttaaaacgaa gaatattttt cctgaatcca
     2521 aaagcaaccg gcagtttgcc gatagcatga aatctattta caggcaaatc gaaaaggtta
     2581 atatggattg ccggctggca gtgtttccaa aagaattgaa taaaaaacgc ctgatcccgc
     2641 tggcttgtgc cgaaggagtc ctgacagggt cctccttata tgccctcaat gagatgtggt
     2701 ataaaaatta cgaccgttcg gcatttcatt ttttcaatga ttcgcgcgaa tggatgcaga
     2761 tggataagct gggccacctg aaggcctcgg taaccatcag taacttttgc tattttgcct
     2821 tccgctggtc gggtgtcaat aagtccaaat cagtgattta cggcagcgct ctcggattgg
     2881 ggtatatgac ggccatcgag gtgctggacg gctattcgag ccattgggga ttttcggtta
     2941 cggatatggc tgtcaatgtg ttgggcacag ccatctttgc ttcgcaggaa atgttgtggg
     3001 aaaaccgccg tgtgaaattc aaaatttcct atcatcctac acaatatgca ggataccgcc
     3061 ccgaagccct gggagataat atcgctgaac gaatgctgaa ggattacaac gggcaaacac
     3121 actggctttc catcagttca ggactgctga tgccgcatca gaaaaaggtt ccgcggtggt
     3181 tgtgtgtctc ctttggttat ggcgccgagg gcatgaccgg cggttttgcc aacccggcgt
     3241 tcaacgaaaa aggcgatgcc ttgccggctt ttgaccggta ccggcagttt tacctttcgt
     3301 ttgatgtgga ttttgcccag ataaaaaccc ggtcgaaatt tgtgcgcggg ctgctgctgg
     3361 cgctgaacac gctgaaggtt cctttcccta cgctggagta caataccaaa ggcgagttta
     3421 agtttcacta tttgtatttt tagtaaagca aactgtacgc gagcgtttgc catttgataa
     3481 tatggaagac agtttcataa acaggctgga gcctgtaaga ataaagacgg cttaggccgg
     3541 agcctgagcc gaacaatgcg ggtcggaaaa tatcgaatat cgattaccga atatcgattt
     3601 atgaagtgaa agacaaaata ttctttggaa aatcataaat cgtcaatcaa tgttcgaagt
     3661 tcaaaagatc ctttaagaaa tttttgtggt gtttgaatat ggagcaatgc aaagtaaaaa
     3721 taatggcgag gctgtctcaa aagtcctttg tgcgccttcg tgccaacctt tgagttactt
     3781 tgtggtaaat acaagtaatt gaaccacaaa gtctgcctga ctgaattcat tcaggcaggg
     3841 atcataaagt gcacacgaaa gttcactaag gttaaatact cacaaatttt gacttttgag
     3901 acagcctctt tgctatcgcg aggatttaaa aatattacta ctaataaatt tgatgctgat
     3961 ttagggcata aattggaaaa tgtagtgtat tttgaacttt tgcgacacgg aggaatggtg
     4021 tttgtaggca aaaacaaaga caaagaagtt gactttatat tgcaaaaagc taataatgag
     4081 cgggggtatt ttcaggtagc atattctgtt aatgacgaaa aaacattgga acgtgaaatt
     4141 tcgtcattta gaagcataaa agacaattat ccccaatatc ttttaacgct tgattatgac
     4201 aatgctgtaa ttgacggaat acaaaagaaa aaaatatttg tattatccgt gcatagattt
     4261 ttttgcaggc aactatatca taactgcata gtcaatgaca tgtcaatgca tttttaatga
     4321 tgttgagata aatcgtgtta gttttttact aatttcattt gcctggtttt gctgtctcc
//
LOCUS       DAOSFP010000191         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18169_length_4360_cov_3.292451, whole genome shotgun sequence.
ACCESSION   DAOSFP010000191 DAOSFP010000000
VERSION     DAOSFP010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18169_length_4360_cov_3.292451"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLI16_06535"
     CDS             complement(<1..1275)
                     /locus_tag="PLI16_06535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH84254.1"
                     /translation="MAAKDKNKKHPQKQYSSKKGAPKIKSAGSTKTVWYALGALILVT
                     LVIYFKAIWFDFLIWDDNMYINLNERIKVLNWDSISKIFSEFYFGNYHPLTTLGYAID
                     YKIAGDAPGLYHFVNILMHLLNTLLVFILIKKIAPKNDYVALITAAFFAVHPLHVESV
                     AWVSERKDVLYTFFFLLSLIFYSDYLKSRQIKNLLLAGLLFLLSCFSKSAAVILPLVM
                     LLFDYYLNRKFTWKTLAEKIPFFAISLVFGVLAMNSQKAAMPVTEEQIIPIIDRLFIV
                     ANSFITYIIKAFVPYKLSALYPYPADLGNGLSVTYYISAIVLALLLFFVWYSRRWGKD
                     IIFGFLFFVITIILVLQIITVGNATMADRYTYVPYIGIFFIVGKLFENLSEKPNKSYK
                     NIVLMTLLAGGIISTAVTFFRIDQWKNDETLFS"
     gene            complement(1662..3374)
                     /locus_tag="PLI16_06540"
     CDS             complement(1662..3374)
                     /locus_tag="PLI16_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84255.1"
                     /translation="MNPQGKNKKIQKKKQQNSPPLPLIEKQAVPKWIWIALGVVLLTT
                     LAIYFRTLSYDLLTHWDDKSYVTDNEHIRGLKWENIKLFFTEYYAGNYQPVTILMYAL
                     EYKFAGADGSLYHLNNLLLHLLNTLLVFVFVRKISPQNAYAALITAAFFAVHPMHVES
                     VAWVSERKDVLYTFFFLISLIIYSDYLRTQKNKHLILSSVFYVLSCLSKPAAVILPLV
                     LLLIDYYAGRKFSKKIILEKLHFFLIAFVIGLVTVKAQGEALHDTSSVPFIQHFSMVT
                     FAFITYIAKAFVPVNLSAIYPYPVDYGGNLSPLYYLTIAVAGLILYFVWHSRKWGKEI
                     IFGFLFFLITIILVLQLKRVGGVTLSERYTYVPYIGIFFILGRLFENHADTAKGKLKK
                     YKNYLLAFLVVCFALFSILTYLRVQIWENDRLLFSDVIKKYPDCGTPHYLLGDHYLDR
                     AAVVADSANRDSNLKQSAANYQDALKCPMNKADKAMVHFNKGCAEFMMKNFQAAYEDF
                     DAAVKLNPKNSLAYGNRGTARYQLGDLQGALQDYNKAIELNPQYTDAINNRENVKQML
                     GIKN"
     gene            complement(3379..>4360)
                     /locus_tag="PLI16_06545"
     CDS             complement(3379..>4360)
                     /locus_tag="PLI16_06545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84256.1"
                     /translation="KAFVPINLSAVYPYPNEIGDTLPIMYYLSIPLCLLLFVLVWYSR
                     KKGKDVIFGFLFFVLCIILVLQFVPVGAAALAERYTYIPYIGVFFIAGRLFDNFATKN
                     NNYRRYGLVIIGLVFIVFSVLSFRRVGVWENGEKLFTDLIAKHPESGISYRLRGDYYL
                     SYYTKKIDVKNTALRSEYLQKTVTDLEKALQYPLNEYNKGCVLYGLGSAYNSRGFEKY
                     SGGDLAGALEEFGKVIKYSPDDAHAYFSMGSIKNETQDYAGALADFDKAISIDNNYFQ
                     AYNNRGSTRFNMKDYHGALEDFEKALALKPDFADAVRNRDMVLSIINSSK"
BASE COUNT         1303 a          902 c          930 g         1225 t
ORIGIN      
        1 cgaaaaaaga gtctcgtcat ttttccattg gtcaattctg aaaaatgtta ccgcagtaga
       61 aatgattcca ccggctaaca aggtcatcag cactatgttt ttatacgact tgttgggttt
      121 ttccgaaaga ttttcgaata atttaccgac aataaaaaat atcccgatat aaggaacata
      181 ggtatagcgg tcggccatgg tggcatttcc cacggtgata atctgtaaca ccaatattat
      241 ggtgattacg aagaataaaa atccgaaaat gatgtctttt ccccatcggc gcgaatacca
      301 tacgaaaaac agtaataaag ccagcacgat ggcagagatg taataggtaa cggataagcc
      361 gtttccgaga tcggccgggt agggataaag tgccgacagt ttgtatggaa caaaagcttt
      421 tataatgtac gtaataaaag agttggcaac aataaaaaga cggtcgataa taggaattat
      481 ttgttcttcg gtcacgggca tggcggcttt ttgtgagttc atggccagca ctccaaaaac
      541 aagcgaaatg gcaaagaacg ggattttttc ggctaaggtt ttccaggtaa acttccggtt
      601 gagataataa tcaaatagaa gcattaccaa aggcaggatt accgcagcag acttgctgaa
      661 acacgaaagc aggaataaca aacccgccag aagcagattc tttatttgcc gtgatttgag
      721 gtaatcggaa taaaaaataa gggaaagcaa aaagaagaac gtgtacagca catcctttct
      781 ctcggaaacc caggccaccg actccacatg caaaggatgt accgcaaaaa aagccgccgt
      841 tattagtgcc acataatcgt tttttggggc aatcttttta ataagaataa aaacaagcaa
      901 agtgttcagc aaatgcatca gtatgttcac aaaatgatac aagcccgggg catcgccggc
      961 aattttgtaa tcaatggcat acccaagcgt ggtaagcgga tggtagttgc caaaataaaa
     1021 ttccgaaaat atcttgctga tgctatccca gttcaacact ttaatcctct cattcaggtt
     1081 gatatacata ttgtcatccc aaatcaggaa atcaaaccat atcgccttaa aataaatgac
     1141 cagggtgacg agaattagag cacctaaggc ataccacaca gttttggtac tgccggcact
     1201 ctttattttt ggggcgcctt tttttgatga atactgtttc tgcggatgct ttttgttttt
     1261 atcttttgct gccattgttt ctttacttgc taatgttatt actttgcggt acgcctggga
     1321 taatttaatg tttcatcact gaccaggaaa ttatttatta atttgagttt tgcttgattg
     1381 tttcaagtca cacagagccg gacacagcgc cgcaaaaaag gttgggtccg tgtcgaagta
     1441 tgacttacaa cagtcgtctt ccctgcctgc gtgccggaca ctccggcagg cagggacaat
     1501 cttctccttc cactttcgtt tcaggagaat ctgctctgac tgaccgtagt ttggttcttt
     1561 ttgacaatat gtaaaaaagt tctttttcat agagttatta tcttggcaat aaacattgat
     1621 ttttatttga caactttatt ttttatgcaa aacttaagtt attaattttt tatcccaagc
     1681 atttgtttga cattttcccg gttatttatt gcgtcggtgt actgaggatt gagttctata
     1741 gctttgttat aatcctgtag cgctccctgc aggtcaccca gttgatagcg tgcggtgccc
     1801 cggttgccat aggcgagcga gtttttggga ttcagtttta cagcagcgtc gaaatcttcg
     1861 taggcggcct gaaaattttt catcataaac tccgcacaac ctttgttgaa atgcaccatt
     1921 gccttgtcgg ctttgttcat agggcatttc agggcatcct ggtagttagc cgccgactgt
     1981 ttgaggttgc tgtctctgtt ggcactgtcg gcaacaacag ccgcacggtc aagataatgg
     2041 tctcccaaca aataatgagg cgtgccgcaa tcggggtatt tctttatgac atccgaaaac
     2101 aacagtcggt cattttccca aatttggacc cgcaaataag tgaggatgga aaacagggca
     2161 aaacaaacca ctaaaaaggc caacaggtag tttttatatt tttttaattt ccctttagcg
     2221 gtatcagcat ggttttcgaa taagcggccc agaataaaaa atatcccgat ataaggcaca
     2281 taagtataac gttccgataa ggttactcct ccaactcgtt tcaattgcaa taccaaaatg
     2341 atcgttatta aaaaaaacaa aaatccaaat ataatttctt tcccccattt gcgagaatgc
     2401 cagacaaaat acagtatcaa acctgccact gcaatagtaa gataatacaa aggtgataaa
     2461 ttgccgccat aatcaacagg gtagggataa atagccgaca gattgaccgg aacaaaggct
     2521 ttggcaatat aggtgatgaa tgcaaaggta accatcgaaa aatgctggat gaaaggcaca
     2581 gaggatgtgt cgtgcagggc ttcgccctga gccttgacgg ttaccaggcc aataacgaag
     2641 gctatcagaa aaaagtgcag tttttcaagt attattttct ttgaaaactt tctgccggcg
     2701 taatagtcta tcagaagcaa cacaagcggc aaaatcactg cggcaggctt gctcaggcat
     2761 gatagcacat aaaaaacaga gctcaggata aggtgtttgt ttttttgcgt tctcaggtaa
     2821 tcgctataaa taatcagtga aatcaaaaag aaaaaggtgt acaacacatc tttgcgttcc
     2881 gaaacccagg ccaccgactc gacgtgcatg gggtgcacag caaaaaaagc agccgtaatg
     2941 agcgcagcat aagcattctg aggggaaatt tttctgacaa aaacaaagac caataaggtg
     3001 ttcagcaggt gaagcaaaag attgttgagg tggtataacg agccatcggc cccggcaaat
     3061 ttatactcca gggcatacat aagtatggta acaggctgat aattgccggc gtagtactcg
     3121 gtgaaaaata gttttatatt ttcccatttc aagccacgga tatgctcgtt gtcggttaca
     3181 taggatttgt cgtcccagtg ggtgagcaaa tcatagctta atgtcctgaa ataaatggcc
     3241 agcgtggtaa gcaataccac tcccaaagct atccatatcc atttggggac agcttgtttt
     3301 tctataagcg gcaggggagg actgttttgc tgctttttct tctggatttt tttatttttt
     3361 ccttgtggat tcataaactt actttgaact gttgatgatg gacagcacca tatccctgtt
     3421 tcttacggcg tcggcaaaat cgggtttcag cgccagggct ttttcgaaat cttccagcgc
     3481 accatgatag tctttcatgt taaaccgggt gctgccgcgg ttattataag cctgaaagta
     3541 attgttgtca atagagatag ccttgtcaaa atcagccagg gcgccggcat agtcctgagt
     3601 ttcgtttttt attgaaccca tcgaaaaata tgcatgcgca tcatcaggac tgtattttat
     3661 cacttttcca aattcttcca gtgctcctgc gagatctcca ccggagtatt tttcaaaacc
     3721 cctgctgttg tatgcactgc caagaccata caatacacag cctttgttgt attcgttcag
     3781 tggatattgc agcgcttttt ccaggtctgt tacagttttt tgcaggtatt ctgaccgcag
     3841 ggcagtgttt tttacgtcaa tttttttggt gtaatagctc agataatagt cccccctcaa
     3901 gcggtatgaa atgcctgatt caggatgttt tgcaatcagg tcggtaaaaa gtttttcgcc
     3961 gttttcccat acacccacac gccggaaact aagcacggaa aacactatga aaaccagtcc
     4021 gataattact aatccgtacc gcctgtaatt gttgtttttt gtagcaaaat tgtcgaatag
     4081 ccgccctgca ataaaaaaca caccgatgta agggatgtat gtatatcgtt cagcgagggc
     4141 tgcggccccc accggcacaa attgcaacac caggatgatg cacagcacaa aaaacaaaaa
     4201 accaaagata acatccttgc ctttttttcg ggaataccat accaaaacga ataacagcag
     4261 gcaaagaggt atcgaaaggt agtacataat tggcagtgtg tccccgattt cgttgggata
     4321 aggatacacg gccgataagt ttataggaac aaaggctttg
//
LOCUS       DAOSFP010000192         4296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18524_length_4296_cov_3.886348, whole genome shotgun sequence.
ACCESSION   DAOSFP010000192 DAOSFP010000000
VERSION     DAOSFP010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18524_length_4296_cov_3.886348"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1227
                     /gene="queA"
                     /locus_tag="PLI16_06550"
     CDS             178..1227
                     /gene="queA"
                     /locus_tag="PLI16_06550"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOH84257.1"
                     /translation="MKLSEFRFHLPAELVADRPAKQRDESRLMVLDRKKQTIEHKVFR
                     DVLDFFQEGDVLIINNTKVFPARLYGKKEKTGAKIEVFLLRELNHESRLWDVLVDPAR
                     KIRIGNKLYFGDNDELVAEVIDNTTSRGRTIRFLFDGPYDEFKKIIESLGDTPLPKII
                     KRQTEPEDKERYQTIYAKHEGAVAAPTAGLHFSRELMKRLELKGVNFAEITLHAGLGN
                     FRTIDVEDLTKHKCDSEQVIINEQACKIVNKAKENKKIVCSVGTTTMKAVESSVTISG
                     MLKPFDGWTNKFIFPPYDFGVANCMITNFHLPQSSLMIMVAAFAGYDFLMKAYKEAVA
                     EKYHFFTYGDAMMII"
     gene            1329..2006
                     /gene="ispD"
                     /locus_tag="PLI16_06555"
     CDS             1329..2006
                     /gene="ispD"
                     /locus_tag="PLI16_06555"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOH84258.1"
                     /translation="MKKIALIVAGGTGKRFGSETPKQFLRICGKPMIIHTLEAFQKAV
                     PDIEFVVVINPVIQDLFDAIFSEFPFPKCIVAEAGPERFYSVRNGLAKITDENSLVAI
                     HDAVRPLVASAVIIQGFRDAEYFGNAIPVVNVPDSVRIKDGPLNQPVDRRVLFKVQTP
                     QCFRTDLIKNAYRQNYHEKFTDDAIVLESCGQRIHLIEGNTENIKITYPFDLLVAEAF
                     LKSIEKE"
     gene            complement(2206..3354)
                     /locus_tag="PLI16_06560"
     CDS             complement(2206..3354)
                     /locus_tag="PLI16_06560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH84259.1"
                     /translation="MTLVLFYIIAFVIALCYCGLILFFTYGWYKNKIRVTHSGSPATR
                     VSVIIAFRNEERNMEQLLKSLSGQHFPAGLWEVILVDDHSSDRSRQIAEAFIRENLKE
                     NFRVISLAKADGFSKKGALKKGIACSQGVLIITTDADCTFGPDYVRTLVGFYEQCRCR
                     VISAPVVFADDNGFFSAMASLEFLSLVASGAGAMSVGQPFMANGANLCFERSLYDEVQ
                     GYAAHQHLVSGDDVFLVLQAKKTVLNRSEICFLKDTKCLVQTMGTSTLKGFFNQRLRW
                     ASKSLAYSDFFASFTAYIVFCFSAVIVIGLAGGLWDYRCAVAGALLLFLKAIVDFPLL
                     YSAASFVKRKRLLLVYLPLQIIYPLYITITGFLSLFVKFEWKGRKASR"
     gene            complement(3351..>4296)
                     /locus_tag="PLI16_06565"
     CDS             complement(3351..>4296)
                     /locus_tag="PLI16_06565"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOH84260.1"
                     /translation="GFIGYRLFCKEDLAGLSNGLLRIVSEPYAIAILAIVLLLMLCNW
                     SVESLKWQYLVRKIEQVSFRKSFMAVWAGLTIGSFTPYRTGEYFGRAFILSKANRWEG
                     VFITFTGSLGQLLATLLFGGIAGFLFVFSLQNGPAADNLSVGYIVAGVATMLALAVFV
                     LYFRISFLKTLFQKLIPARFQRFRAHFDVYSYYSFQELLTAFVFSVFRYVIFSMQFYI
                     LMRLCNIDIPLADGLMVVSCIFLAMSAIPTFALSEIGVRGSVSILLLELYFENLSQQP
                     EGLASAAVLASFLIWFINLIIPALIGVIFVFRLKFFRN"
BASE COUNT         1374 a         1034 c          856 g         1032 t
ORIGIN      
        1 tgaaaatttc atttaacaac aatactgtta ccagaaaaat cgttaagttg taattacttg
       61 aattttaaaa tattttagac gttttcctga aatttttccg ggaaaacttg atttttagca
      121 aataaaagcg tatctttgca ctcttttttt gtaaaatata tattatatta atttcctatg
      181 aaattatcag aattcagatt tcatttacca gccgaactgg ttgccgacag gccggccaaa
      241 caaagagatg aatcgcgcct gatggtactg gaccggaaaa aacaaactat tgagcacaag
      301 gtattcaggg atgtgctgga tttttttcag gaaggcgatg tattgatcat taacaacacc
      361 aaggtatttc ccgcccgcct ctatggcaaa aaagaaaaaa ccggcgccaa aatcgaagta
      421 tttttgctgc gcgaacttaa ccacgaatcg cgcctgtggg acgttctggt tgatcctgca
      481 cggaaaatac gcataggcaa caagctttat ttcggcgaca acgacgaact ggttgctgaa
      541 gtcatcgaca acaccacctc aaggggccgt acaatcaggt ttttgttcga cgggccttat
      601 gatgagttta aaaaaattat tgaatcgctg ggcgacaccc ctttgcccaa aattataaaa
      661 cggcaaaccg aacccgaaga caaagaacgg tatcagacca tttacgccaa acacgaaggc
      721 gccgttgccg cacccaccgc cggcctgcat ttcagccgcg aattgatgaa acgcctcgaa
      781 cttaaaggag ttaattttgc tgaaattacc ctgcatgccg gattgggaaa tttccgcacg
      841 atcgacgtgg aagacctcac caaacacaaa tgcgactccg aacaggtaat tataaatgaa
      901 caagcctgta aaatcgtcaa caaagccaaa gaaaacaaaa aaatcgtttg ttccgttggt
      961 accaccacca tgaaagccgt ggaatcatcg gttactattt caggcatgtt aaaaccattt
     1021 gacgggtgga caaataaatt tattttcccc ccatacgatt tcggagttgc caactgtatg
     1081 ataacaaatt tccatcttcc gcaatcttcg ctgatgatta tggtggccgc ctttgcaggt
     1141 tacgattttc tgatgaaggc ctataaagaa gccgtagctg aaaagtacca ctttttcacc
     1201 tatggtgatg ccatgatgat tatttagtgt ttgtatagac acgatttaac aaaacttttt
     1261 tttcataata aagtcgttca agcctttttt ttgaacgact tttattattt ttgaacaaat
     1321 ctatcggctt gaaaaaaatt gcactcatag ttgccggggg taccggcaaa cggtttggct
     1381 ctgaaacacc aaaacaattc ctgaggattt gcggcaaacc gatgattatt catacccttg
     1441 aagcattcca aaaagccgtg cccgatatag aatttgtggt agtaatcaac cccgtaatcc
     1501 aagacctttt tgacgccatt ttttcggaat ttccctttcc caagtgtatt gtggccgaag
     1561 caggccccga acgtttttat tctgtgcgca acgggttggc aaaaatcacc gacgaaaact
     1621 ccctggtggc catccacgat gctgtcaggc cattggtagc ttccgccgta atcatacagg
     1681 ggtttcgcga tgcggaatat ttcggcaatg ccataccggt ggtaaacgtt cccgactcgg
     1741 tgcggataaa agacgggccg ctgaaccaac ccgtagaccg ccgggtactt ttcaaagtac
     1801 aaactccgca atgtttccgt accgatctca taaaaaatgc ttaccggcaa aattatcatg
     1861 aaaaatttac cgatgatgcc attgtgctcg aatcctgcgg acaacgcatt cacctgatag
     1921 aaggcaacac tgaaaacata aaaataacat atccttttga tttgttagtg gcggaagctt
     1981 ttctgaaaag cattgaaaaa gaataaggtt tcactgccca gtaaggaact gtttcgaaaa
     2041 taatatattt ctgttagccg cagactaaca tttacgcggt gttaagttcg ggaacttcgc
     2101 acaaatggta cccgaggcat cctgcatgcg tgttattctg ccgccatcat caacgaccgg
     2161 tacgaattta acatcaagaa ataataccaa tactcctccg cgggcttagc gagaggcttt
     2221 tcgccccttc cattcaaact ttacaaacag ggataaaaaa cccgtaattg ttatgtacaa
     2281 gggatatatt atttgcagcg gcaagtaaac caacaaaagc cgttttcttt ttacaaatga
     2341 agccgccgaa tacaaaagcg gaaaatcaac aatcgccttt aggaacagca acaaggcgcc
     2401 ggcaacagca caacggtaat cccacaagcc gcctgccagc ccgattacaa taaccgcgct
     2461 aaaacaaaaa acaatataag ccgtgaaaga ggcaaaaaaa tcgctgtatg ccaggctttt
     2521 tgacgcccag cggagacgct ggttgaaaaa acctttgagg gtgcttgtcc ccatggtctg
     2581 caccaggcac tttgtatctt tcaaaaaaca gatttccgac ctgtttaaaa ccgttttttt
     2641 tgcctggagc accaaaaaca catcatcgcc ggaaaccaaa tgctgatggg cggcatagcc
     2701 ctgcacttca tcgtacaggc tgcgttcaaa acacaagttg gccccattgg ccataaaggg
     2761 ctggcctacc gacatcgccc ctgccccgct ggccacaagg ctcagaaact ctaacgaagc
     2821 catagccgaa aaaaaaccat tatcatcagc aaaaaccacc ggcgctgaaa tcacacgaca
     2881 gcggcactgt tcgtaaaaac caaccagggt gcgcacataa tccgggccaa aggtgcaatc
     2941 agcgtccgta gtaataatca gtaccccctg cgaacaggca ataccttttt ttaaagcacc
     3001 tttttttgaa aaaccatccg ctttggctaa agaaatcacc ctgaaatttt cttttaaatt
     3061 ttcccggata aatgcttcgg caatttgccg gctgcggtcg ctggaatgat catctaccag
     3121 tatcacttcc caaagccctg ccggaaaatg ttggcccgat aaacttttca gcagttgctc
     3181 catgtttctt tcttcgttgc gaaaagcgat aatcacgctg acccgtgttg ccggactgcc
     3241 ggaatgagta acacggattt tgtttttata ccacccatag gtaaaaaaca gtatcagccc
     3301 acaataacag agggcaataa caaaagcaat aatataaaat aaaaccagtg tcaattcctg
     3361 aaaaatttaa gacggaatac gaaaattacc cctatcaggg caggaataat caaattgata
     3421 aaccaaataa gaaaactagc caatactgca gcagaggcaa gaccttcagg ttgttgcgac
     3481 aggttttcaa aatacagctc cagcaacaaa atgcttaccg agcctctgac gccaatctcg
     3541 ctgagggcaa atgtgggaat agccgacatg gccagaaaaa tgcaactcac caccatgagc
     3601 ccgtcagcca acggaatatc aatattacat aacctcatca ggatataaaa ctgcatgctg
     3661 aaaataacat accggaatac gctgaataca aacgcggtca acagctcctg aaaagaatag
     3721 taagaataca catcgaagtg tgccctgaac cgttgaaacc tggccggtat cagcttctga
     3781 aacagtgttt ttaaaaaaga aatcctgaaa tacagaacaa aaaccgctaa ggccaacatg
     3841 gtggctactc cggcaacgat ataaccaaca ctcaggttgt ctgccgcagg accgttttgc
     3901 aaggaaaaaa caaacaaaaa ccccgcgata ccaccaaaca gtaatgtggc caaaagctgc
     3961 ccaagacttc cggtaaaagt aataaacact ccttcccagc ggttagcctt tgacaaaata
     4021 aaggcccgcc cgaaatattc gccagtgcgg taaggtgtaa aagaccctat ggtaagcccg
     4081 gcccacacag ccataaaaga ctttcgaaac gaaacctgct cgattttccg caccaggtac
     4141 tgccatttga gagattccac actccaattg cacaacatca acagcaatac gatggcgagt
     4201 atggcgatgg cgtagggctc cgacactatc ctcaacaagc cgttactgag gcctgcaaga
     4261 tcttctttgc aaaataaacg atatccaata aagcca
//
LOCUS       DAOSFP010000193         4281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18612_length_4281_cov_4.301704, whole genome shotgun sequence.
ACCESSION   DAOSFP010000193 DAOSFP010000000
VERSION     DAOSFP010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18612_length_4281_cov_4.301704"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(675..1472)
                     /locus_tag="PLI16_06570"
     CDS             complement(675..1472)
                     /locus_tag="PLI16_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOH84261.1"
                     /translation="MDKKIKIEVPVIADLAAKGENPEYLYWVGCAGAFDDRYKKVSRA
                     FIKILTHFNISYAVLGVEESCTGDPARRAGNEMLFQMQALTNIETFKRYGIKKIITTC
                     PHCFNTLKNEYPDLGGEYEVVHYTQFLQQLKNKGILKISDNIFLGKTITFHDPCYLGR
                     ANCEYEAPREVIKTLSTHNKEMKRNKSFALCCGAGGAQMFKEAEKGEKEIFIERTEEA
                     LETGADIIATACPFCMTMLTDGLKYKNKEEEVKNLDIAELIAEALNL"
     gene            complement(1542..1895)
                     /locus_tag="PLI16_06575"
     CDS             complement(1542..1895)
                     /locus_tag="PLI16_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH84262.1"
                     /translation="MKFKFENLIIWQMAMDFGESINKLSSRFPKSEMYNLSSQISRAV
                     DSIALNISEGSIGQSKPEFRKFMGYSIRSLAEVVTCLHKAFKRNYITNQEFNEHYEFA
                     FNLMNMMVAFKEKIK"
     gene            complement(2032..3342)
                     /locus_tag="PLI16_06580"
     CDS             complement(2032..3342)
                     /locus_tag="PLI16_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOH84263.1"
                     /translation="MIKQIIFIIALAITLGVFFYTISRLYKFFGFTKPFALGTWGKRF
                     RVMFKVAIFQSKIFRRPVIGFFHAMVFWGFCVILIGSIEMVIDGVSGSERVLSCLGVF
                     YDIITASGDIFALIIIVAIIVFLVRRLVIKVKRFEGIEMKPKNHWDAAMALTMILILM
                     ISLLGMNTFYLTSCEFLKHDVHGTYPVSSFLAGWMGVGEEDYDFYQLFWWIHILLIFV
                     FANILPYSKHFHIFLSVFNVLFVRLDPLGKLPNMPSITREVKMMLDPSLANAENSAEP
                     AIERFGIKDVEDGTWKNYFDSLTCTQCGRCTSVCPANITGKKLSPRKVMMNYRARMKE
                     KGPGMIKQGKEYDDKKSLINDDYLSLEELWACTTCNACAKECPLMINQPELIMGMRRY
                     LVMEEGNAPAQLKTMFANIENNGAPWQFSPEDRMQWAEDLVLKG"
     gene            complement(3361..4140)
                     /locus_tag="PLI16_06585"
     CDS             complement(3361..4140)
                     /locus_tag="PLI16_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84264.1"
                     /translation="MRNILVVLMLSLLMVSCSQGPNEKGEYVIKPGGDAKEIHVKYNA
                     NGNLEYIQQYAGEKAEGIYLNFHDNQQPDNLTFIKEGKNHGTGLVFHKNGMLNNVGNY
                     VDGERSGWFYVFDRNSVLTGKREYIVLDGKSYLNQWIEYDRNGLPDKMNSSYLNVKAP
                     KDTIKNGEEFQLSVALEASFFKQYMLLIVGPFDEKYQLPANATCDTIKSNNYNALYKT
                     KSYKKGKNVIRGMVQEIKLKDNESAYEVRKIYFSTEFFVSK"
BASE COUNT         1178 a          950 c          781 g         1372 t
ORIGIN      
        1 ggacggttgc agggaagggc agccacactc ttgcttttgt gggctgggtt ttcctctttt
       61 tctttgtctt tatgcagcaa attttccatt gtcgttgtct tttttccgct tgtgcctaac
      121 ggttggcttg caccaacagg tagctatcgg tccgatagct atcggatagt gttattttta
      181 ctatatcttt ggaattactg ttatccattt ttaggcttat aaacatttgt aaattaacat
      241 attaattttt aaattaaaat aattgtaaat gattgtaact gcttaaaaag cttaataact
      301 atttcggaca aatatataaa aataaatttc aaatcaaaat attttatcat attatttttt
      361 ttcaaaacat ctcttcagtg ctctgattta ttataccatt tgtgcttatt ttttagtcta
      421 ccttaaaaaa taagctacaa tggtaaatgc cgatgctaca tgaaaatagt acaatgagat
      481 gcagcgaaat tggactatat tgaatgtgct atcggtatta ataatagagt ttatcacaat
      541 tacgcgatat aaattataag cattgaaaat aaaatagttg agacagactt aaaggtttgc
      601 acaaagtctg acaatgtact tttgagacat cctcctaaac atgaaccaat actcagggct
      661 tcagcacatt tcgattacag attcagtgct tcggcaatca actccgcgat atcaaggttt
      721 ttcacttctt cttccttgtt tttgtatttc agcccgtcgg tgagcatggt catgcagaac
      781 gggcaggcgg tagcaataat atctgcgcct gtctccaacg cttcttctgt acgttcaata
      841 aaaatttcct tctccccttt ttcggcttct ttgaacatct gtgctcctcc tgccccacaa
      901 cacaaagcaa agctcttatt ccttttcatt tctttattat gtgttgaaag ggttttaatc
      961 acctcccggg gcgcctcata ttcgcagttt gcgcgcccaa gatagcaggg gtcatgaaag
     1021 gtaatggttt tacccaaaaa aatattgtcg ctgattttca ggatgccttt gtttttcagt
     1081 tgttgcaaaa attgagtgta gtgcaccact tcgtattcgc caccgaggtc gggatattca
     1141 ttttttaagg tattaaagca gtgcgggcaa gtggttatga tttttttaat accatatctt
     1201 ttaaaagtct caatattggt gagtgcctgc atctggaaga gcatttcgtt accggcacgc
     1261 cgtgcgggat cgccggtgca gctttcttcc acacccaaaa cggcgtagct gatattaaaa
     1321 tgtgtgagta ttttgataaa ggcgcgggaa acttttttgt atcggtcgtc aaaagcgccg
     1381 gcacagccta cccaataaag atattcagga ttttcgcctt tggccgcaag atcggctatc
     1441 acaggaactt caatttttat ttttttatcc atcagggaac tttttattgt ttggagggtt
     1501 tttattaatg gggtttttaa gtcagaagtt agcagttgga gttattttat tttttcttta
     1561 aatgcaacca tcatgttcat cagattgaat gcaaattcgt aatgctcatt aaattcttgg
     1621 tttgtgatat aatttctttt gaatgcctta tgaaggcagg taaccacttc ggccaaagac
     1681 ctgatagagt aacccataaa ctttctgaat tcaggttttg actgtccaat ggagccttct
     1741 gaaatgttca gagcaatgga atctactgcc ctgctaattt gcgaactaag attatacatt
     1801 tcacttttag gaaaacggct agaaagctta tttatgctct cgccaaaatc catcgccatt
     1861 tgccatataa ttaagttttc aaatttaaat ttcattttga taattttatt gttttttaaa
     1921 taattgtctt tcgttccact ttcacccccc cccacttccc gcttcctact tcccgcttcc
     1981 tacttcccgc ttcctacttc ccgcttccta cttcctgctt ccctctcccc ttcacccctt
     2041 taaaaccaaa tcctccgccc actgcatcct gtcttccggc gaaaactgcc agggggcgcc
     2101 gttattttca atatttgcaa acatggtctt aagttgggcg ggcgcattac cctcttccat
     2161 caccaggtaa cgccgcatcc ccattatcag ctcgggctgg ttgatcatca gcggacattc
     2221 cttggcacag gcattacagg tggtgcatgc ccagagttcc tccaaactta aataatcatc
     2281 attgatcagc gactttttat catcatactc cttgccttgc tttatcatgc cgggaccctt
     2341 ttctttcatt cgggcacggt aattcatcat gaccttgcgg ggcgaaagtt tcttccccgt
     2401 aatgttggca gggcacaccg aagtgcatct gccgcattgg gtacacgtca aagaatcgaa
     2461 ataatttttc caggtaccgt cttctacgtc ttttatccca aagcgttcta tagccggctc
     2521 tgcactgttt tcagcattag caagagaagg atcaagcatc atctttactt cgcgtgttat
     2581 acttggcata tttggaagtt ttccgagcgg gtctaacctg acaaacaaca cattaaacac
     2641 cgacaaaaag atgtgaaaat gtttggaata aggcagtatg ttggcgaata caaaaatcag
     2701 cagaatatga atccaccaga aaagctggta aaaatcatag tcttcttcac caacgcccat
     2761 ccagcctgca agaaaagaac ttaccgggta tgtgccatga acatcgtgtt ttaggaactc
     2821 gcaggaagta agataaaagg tattcatccc cagcagtgat atcatcagaa taagaatcat
     2881 cgtaagcgcc atggctgcat cccagtggtt tttcggcttc atttcgatac cttcaaagcg
     2941 ctttactttt ataactagcc ttcttaccag gaaaacaatt attgccacga taatgatcag
     3001 ggcaaagata tctccggatg ccgtgataat atcataaaac acccccaggc aggacaacac
     3061 acgctctgac cccgaaacgc catcgataac catctcgata ctgccaatga gtattacgca
     3121 aaatccccaa aacaccatgg cgtggaaaaa gcctatcacc ggacgacgaa agatttttga
     3181 ctgaaaaatc gccactttaa acataacacg aaatcgcttg ccccaggtgc ccagagcaaa
     3241 tggcttagtg aaaccgaaaa acttgtacag acgggatatc gtgtagaaaa aaacacccag
     3301 cgttattgcc agagcaataa taaatatgat ttgctttatc ataaaattct gtttaccgtg
     3361 ttatttactg acaaaaaact ctgttgagaa gtatatctta cgaacttcgt aagcgctttc
     3421 attatctttt aattttatct cctgcaccat ccccctgata acatttttac cttttttata
     3481 actctttgtt ttgtacaatg cgttgtagtt gttgctcttg attgtatcgc aggtcgcgtt
     3541 ggccggcagt tggtattttt cgtcaaaagg accaacaatc agcaacatgt actgtttaaa
     3601 aaaggatgct tccagagcaa ccgacagttg aaattcttcg ccgtttttta tggtgtcttt
     3661 gggggccttc acattcaggt agctgctgtt catcttatca ggcaagccat tccggtcgta
     3721 ctctatccac tgattcagat agcttttgcc atccaggact atatactcgc gctttcctgt
     3781 aagcacgctg tttctgtcga aaacataaaa ccatcccgaa cgttcaccat ctacataatt
     3841 tccgacattg ttgagcatgc cgtttttatg aaaaaccagc cctgtaccat gatttttccc
     3901 ctctttgata aaagtaaggt tatcaggctg ctgattatcg tgaaaattca ggtaaatccc
     3961 ttcggccttt tcgccggcat attgctgaat gtactcaagg ttgccgttgg cattgtattt
     4021 tacgtgtatc tctttggcat cgccaccggg cttaatgaca tattcgcctt tttcattcgg
     4081 gccctgcgaa cacgacacca tgagcaggga aagcataagt acaacaagga tatttctcat
     4141 tttttgtgtt tttttgatga tgtaaaatta caacttttca gttataacga tgttttttga
     4201 aatttcacct aaaacatatg cacacgtcat atctgactaa atagtcaatc gatattactt
     4261 ctgccccaac ataaaagaaa a
//
LOCUS       DAOSFP010000194         4277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18646_length_4277_cov_4.013264, whole genome shotgun sequence.
ACCESSION   DAOSFP010000194 DAOSFP010000000
VERSION     DAOSFP010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18646_length_4277_cov_4.013264"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLI16_06590"
     CDS             complement(<1..911)
                     /locus_tag="PLI16_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84265.1"
                     /translation="MKKFLLIILILLASIHARSQLFYNNGALVSVRPGGVMQINGSIQ
                     NSNAGTLQNNGDLTVTADITNNAVAAGDGIYRLAGNWTNNGTFTPALSTVIFNGNTLQ
                     TIGGTVLSTFNNLTINNPLSGVTLSNIDAEVNNTLTLTSGRLNVASANLTLNSTATAV
                     AGSPFTAANMVVVNQGGELRKKFTSTGMYFFPVGDNAGTAEYSPVAVNITAASGFSNA
                     YVGIKVNDIKHPDNNSTIHYLKRFWSITQAGIASCLASINANYVTADISGTEASIKSA
                     QLNGTFNQLTNPWVKYVTLTSNTLTASG"
     gene            complement(923..2395)
                     /locus_tag="PLI16_06595"
     CDS             complement(923..2395)
                     /locus_tag="PLI16_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84266.1"
                     /translation="MKKLVFLNLLLAVLFCPQLLAQQNVGIGTNTPDNSALLDLTSVN
                     KGLLVPRMTTAQRTAITAPATGLLVYDSNLQQFWYYNGTQWTVVAGGGGGSCYTLQQS
                     YDCGGPGAGRNIVVSGTNSVNITNANASSIGLRSTHSADGVSIKAENTYANVQYAAVQ
                     ASTASNFGTVGGTPVPTTAIIGNSTGKAFGVSGQVESTGTGQAGIFGNNLRTNGGHGV
                     YGIGFNGAVGESNYETGFGLFGLNHGTAVNGNAVGTIGQGYVGVWGETDDGPAAGVYG
                     QNVSASVVDDNIGVWGYGWIGVFGETNDVSTGYGLFSSGNSGASGTKSFAIDHPLDPG
                     NKILKHFSMESPEVLNLYRGTASINNNGEALVVLPDYFDQININFSYQLTAVGAPAPG
                     LYIKQEINGGKFIIAGGIPGTKVCWTVYAERNDKFLQQHPEAKLTEVMKRKSNTYLMP
                     ELWNQPAERGVFSGCPKTQKGQLLVPESKFHQEEMKLINE"
     gene            3128..4078
                     /locus_tag="PLI16_06600"
     CDS             3128..4078
                     /locus_tag="PLI16_06600"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOH84267.1"
                     /translation="MSSDRIMVFSGSGSRKLTGSICDYLQTEQGKSEVLHFSEGNTFV
                     RINENVRGCHVYIVQSIVYPANDNLVELLFWIDAFKRASAESVTVLIPFFSYSKGDKK
                     DEPRVSIRARVCADTIEVAGADRIVTMDLHSPQIQGFFRKPVDNLYALGVLCEKIKSL
                     ETKDYVIVSPDTGFAGQARKFASYLKAPVVIADKQRLRHDENAEITEIIGNVQGKTAI
                     VVDDMIISGGTLKDVSEKLIQRGAKQVYAMATHGVLSPGSEKKLDDSPIKKLFITDTV
                     ENQPEKFSSKIEVISVAAVFGEAIKRIHNKESLSDIFTVK"
BASE COUNT         1267 a          915 c          862 g         1233 t
ORIGIN      
        1 ccagaagcag tcagggtatt agaggttaaa gtaacatatt taacccaggg attggtaagc
       61 tggttgaaag tgccgttcag ttgtgcgctt ttaatgctgg cttctgtgcc agaaatatcc
      121 gctgtaacgt aattagcatt tatggaagcc aaacaggaag ctattcccgc ctgagtaata
      181 ctccaaaagc gcttaagata atggatagtg ctattgttgt cggggtgttt gatgtcgtta
      241 actttaattc ccacataagc attgctaaag cctgaagctg cagtgatatt tactgccaca
      301 ggtgaatatt ccgctgttcc ggcattatca cctacaggaa aaaagtacat acctgtggaa
      361 gtgaattttt tacgcagttc cccaccctga ttgaccacta ccatattggc cgcagtaaaa
      421 ggacttcctg ccacagcggt tgcggtgctg tttaaggtca ggttggccga agccacattt
      481 agcctacccg aggtaagagt cagggtatta ttgacttcag catcaatatt tgatagcgta
      541 acacctgata aagggttgtt tatggtaagg ttgttaaatg tagataacac ggttcctcca
      601 atggtctgta gcgtgttgcc attgaaaata acagtgctta gtgcgggagt gaatgtcccg
      661 ttgttagtcc agttacctgc cagacgatat atgccatcgc cagcggcgac agcattattg
      721 gtaatatcag cagtaacggt taaatcgccg ttgttttgca aggtgccggc attgctgttt
      781 tggatgctgc cgttaatttg catgactcca cccggcctga ccgataccag cgcaccgttg
      841 ttataaaaca actgcgacct ggcgtgtatg gaagccagca atattaagat aattaacaaa
      901 aattttttca tagatatgct ggttattcgt tgatcaactt catttcttcc tgatgaaatt
      961 tagattcagg caccaagagc tgtccttttt gtgttttcgg gcatccgctg aaaactcctc
     1021 tttctgccgg ctggttccat aactcaggca tcaggtaagt atttgatttt cttttcatca
     1081 cttcagtcaa ttttgcttcc ggatgctgtt ggaggaattt atcgttacgt tcagcataca
     1141 ctgtccagca aactttagta cccggtatgc cgccggcaat aataaattta ccgccgttga
     1201 tttcctgctt aatataaagt cccggtgccg gtgcgcccac tgccgttagc tgataagaaa
     1261 aattgatatt tatctggtca aaataatccg gaagtacaac cagagcttct ccgttgttgt
     1321 taatgctggc tgtgccccta taaagattca gcacttcggg tgattccatg gaaaaatgct
     1381 ttaaaatttt gttgcccgga tcaagggggt gatcaattgc aaaagatttt gttccggagg
     1441 ctcctgaatt tccgctacta aataatccat atcctgtact cacatcgttg gtttctccga
     1501 aaacgcctat ccagccataa ccccagacac ctatattatc atcgactacc gaggcactga
     1561 cattttgtcc gtatacgcct gctgccgggc catcatcggt ttcaccccaa acaccaacat
     1621 atccctgccc tatagttccc acagcatttc cgttgacagc cgtaccatga ttcaagccaa
     1681 aaaggccaaa gccggtttca taattgcttt caccaacagc accattaaat cctatgccat
     1741 aaacaccatg tccgccgtta gttctaaggt tatttccaaa gatacctgcc tgtccggtac
     1801 ccgtggactc cacctgaccg ctgacaccaa aagcttttcc ggtggagttg ccgataatag
     1861 ccgtagtagg taccggcgta cctcctactg taccgaagtt agaggcagta gaagcctgaa
     1921 cggcagcgta ctgcacgttg gcataagtat tttcggcctt tatggaaacc ccgtcagcgg
     1981 aatgtgtaga acgcaacccg atggagctcg cgttagcatt ggtaatatta acagaatttg
     2041 ttccgctgac aacaatgttt cttccggcac caggaccgcc acagtcgtag ctctgctgca
     2101 gggtataaca cgatcccccg cccccgccgg caacaacagt ccattgagtg ccattataat
     2161 accaaaactg ctgcaagtta ctatcgtaaa ccagcagccc ggtagccggg gccgtgatag
     2221 ccgttcgctg cgccgttgtc attcggggaa ctaataaacc tttgttgaca gaagtaaggt
     2281 caagcaatgc cgaattatcc ggagtgttgg tacctatccc aacattttgc tgagccaaaa
     2341 gctgcggaca aaataatacc gcgagcagta aatttaagaa gacaagtttt ttcatgttgt
     2401 attaatttta ataattattc ataggtacaa agataaatta ttaatgtact aatacaggta
     2461 ttcatatgat gaaagactta aaaaaaacaa ttgaaaatct tatctgcaac tgctaacaat
     2521 aaaatgaagc actattttta actttttatc caaataaata attattttca ttcttatatg
     2581 aaatcatgtg tcataacata tgtcatacaa ctcatagcac attcatttta aagtcaaagt
     2641 tttattaatt ttatcgtaaa aaccatataa cacataaacc atatcaacat ctttttccgt
     2701 aattgtttta cttagtaaaa agtaaaataa tgagaaccat tatttttatt tttttaattt
     2761 ttcatagaaa ttttttcctg aaaaattaac tttgtaatta atgctcctta cttttatttt
     2821 taagatttcc actttaaata acatttgttt tggattcaag gtactctttg atatgcagaa
     2881 ggccatacta agccctgata gaaggactgg aaatcatgtc ttgtccagaa ttggctcata
     2941 gtataagaaa caccgggaaa agaacaacag taatttattg gttgataccg atttatcttt
     3001 tctttataaa gataacataa aaatcaatgt ttctttagag aacaaaattt gtgtttttca
     3061 ttgcaaaacc tgaaatatat tcataccttt acacataaga tttattaacc aataaaacat
     3121 ttagcacatg agttctgaca ggataatggt attttcgggc agtggcagtc gtaagcttac
     3181 cggcagtatt tgcgattatc tccaaacgga acaagggaaa agcgaagttt tacacttttc
     3241 ggaaggtaat acttttgtca ggataaacga aaacgtcaga ggatgtcatg tttatattgt
     3301 acaatcaata gtgtatcctg ccaacgacaa cctggtggaa ctgctgtttt ggattgatgc
     3361 ctttaaacgt gccagtgctg aatcagtaac tgtattaatt ccatttttca gttactccaa
     3421 aggcgacaaa aaagacgagc caagggtgtc catcagggcc cgcgtatgtg ccgacactat
     3481 tgaagttgcc ggtgccgacc gtatcgtcac catggacctg cactcaccgc aaattcaggg
     3541 tttctttcgt aaacccgtcg acaaccttta tgctcttgga gtgctctgtg aaaaaataaa
     3601 atctctggaa accaaggact atgttatcgt ttcgcctgat accggctttg ccggacaggc
     3661 aaggaaattt gcttcttacc tgaaagcccc ggtggtaata gccgacaagc aacggttacg
     3721 tcatgatgaa aatgcagaga taacagagat tattggcaat gtgcaaggca aaactgctat
     3781 tgtggtagat gatatgatca tctctggagg cacactgaaa gatgtgtcgg aaaaactaat
     3841 tcaaagagga gctaaacagg tttacgcgat ggcaacgcac ggagtactgt cgcccggatc
     3901 agaaaaaaaa cttgacgaca gccctattaa aaaacttttc atcaccgata ccgtggaaaa
     3961 tcaaccggaa aaattttcct ccaaaattga agtgatatca gtagctgcag tatttggcga
     4021 agccataaaa aggatacata ataaggaaag tttgagtgac attttcactg taaaataggt
     4081 ccaaaattca ggaccgtact ttagcaaata gaataccagt acgccaaaac gcccagtcac
     4141 agttgctatg gaataaaatc tattcccgct ttacgggctt gcgaatgctg aaaattccgg
     4201 ttcacaaggt acaggcggcc ttctttacga aacctggcac cggtctgttt aaaatggaat
     4261 gcaccttttt ttaacat
//
LOCUS       DAOSFP010000195         4269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18694_length_4269_cov_3.935928, whole genome shotgun sequence.
ACCESSION   DAOSFP010000195 DAOSFP010000000
VERSION     DAOSFP010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18694_length_4269_cov_3.935928"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLI16_06605"
     CDS             <1..581
                     /locus_tag="PLI16_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOH84268.1"
                     /translation="SGTFADAEKILSAINDTSKLKLAFAKEGYGPSDHASFYADSIPV
                     LYFNTGVHPDYHLPADDVEKINCDGLSVISDFVVKLIDELSLKTYDLAFREAGSRTKG
                     SQRMGLKVTLGIMPDFARQEVKGVAVGAVNADGPAQRGGMKKGDVITAIGEKTVNDIY
                     EYMERLKTLSAGSLITVDVMRNGKKEVLLIQL"
     gene            595..1422
                     /locus_tag="PLI16_06610"
     CDS             595..1422
                     /locus_tag="PLI16_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84269.1"
                     /translation="MEQIVNSRKKMVMAWALAIIITLAAGWYQRVTGPSHPLKVNAGI
                     NGKSYVFSLARSGSSSDNEIQVPVTDKDVKGYVIYRRYPTNENWDTLAMQPVKEGLSA
                     FLPVQPPAGKLEYFLMFRTTGQPDMLLQQQPTIIRFKGDVPLWILLPHILFIFFGMLF
                     SNLAGLLAAFKFNRARIYSLITFILIMLGGMILGPVVQKYAFGEFWTGVPWGWDLTDN
                     KTLLAFLAWLAALFFNRNKLRPGYIIAAALVTLIIFSIPHSMFGSELNPATGTITQG"
     gene            complement(1468..2922)
                     /locus_tag="PLI16_06615"
     CDS             complement(1468..2922)
                     /locus_tag="PLI16_06615"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOH84270.1"
                     /translation="MTLRIQLVKEISENSHIIIICPTETQLLKTAANRLNKAEQAYLK
                     ARIKKTKKGIITIDRLGHYLFFYLLSTGKKAPHLVSEEMRKAGHGMLTLAEEHHLETL
                     TLYPQGLQEAHIEAFVEGMALSAYQFIKYRSNVKEEELKLKTLKVCSKLFGEKHLQRL
                     NVLTEAVHICRDMVNEPVCHMNSQVFSAQAMDLLKNSGARVEVLSKAKIAAYRMNGLL
                     AVNRGSIDPPAFLIMEWEPTHPVNGKPLVLVGKGLLYDTGGINLKTSVHMENMKNDMA
                     GGAAVVSALYAVAKTKLPVHVIGLVPLTDNRPAGNALVPGDIIQYSDGTTVEVLNSDA
                     EGRLILADGMIFAKKFHPSLVITIATLTGSAHSAIGKYGMVGMHQQAQKHFKKLQSAG
                     DSAFERIVEFPFWEEYDELIKSSIADIKNTGGPYGGAITAGKFLAHFAKYPFIHLDIA
                     GPAFNDKKDSYRGTGGSGVGVRLLYEFIKRLASR"
     gene            3055..4140
                     /gene="mqnC"
                     /locus_tag="PLI16_06620"
     CDS             3055..4140
                     /gene="mqnC"
                     /locus_tag="PLI16_06620"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HOH84271.1"
                     /translation="MKAIKILDKALKTDAISGAEALYIYEKAALPDLIFVANEIRKKM
                     HPGNTVGWIIDRNVNIANVCVSQCLFCNFCRKKNQPDAYITSLDQYRQKIDELFALGG
                     NQLLLQGGMHPDLGLDFYVDLFSTLKSFYPLLKLHALGPPEIVFIAGKEKKSFREVLE
                     KLVEAGLDSLPGAGAEILSDRVRKLISPAKCSSKEWLDVMREAHKLNLPTSATMMFGH
                     VENIQERIEHLIKIRQVQDEKPPAAYGFLSFIPWPFQQSGTRLITKFHITEMVSHNDY
                     LRFIAISRIVLNNIPNIQPSWLTVGVPLAQLCLHAGGNDFGSLMIEEHVVAQAGVSRR
                     LASREMLQAITGAGFKPRLRNQKFEYF"
BASE COUNT         1095 a          963 c         1015 g         1196 t
ORIGIN      
        1 gtagcggcac tttcgcagat gcggaaaaaa tattatcggc catcaacgat accagcaaac
       61 tgaaattggc atttgccaaa gaaggctatg gaccttccga ccatgcttca ttttatgccg
      121 acagcattcc tgtgttgtat ttcaataccg gtgtccatcc cgattaccac ctgcccgccg
      181 atgatgtgga aaaaataaat tgcgatggct tgtcggttat ttctgatttt gtggtgaagc
      241 taattgatga actaagcctg aaaacttatg accttgcttt tcgcgaagct gggtcccgca
      301 ctaaaggtag tcagcgtatg ggtctcaaag ttacgctggg tatcatgccc gactttgcac
      361 ggcaggaggt caaaggggtg gccgtgggag ccgtcaacgc tgatgggccg gcacagcgcg
      421 gaggtatgaa aaaaggcgac gtgataaccg ccataggcga aaaaacagta aacgacattt
      481 atgaatatat ggagcggctg aaaacattga gcgccggaag tcttataact gttgatgtga
      541 tgagaaacgg caaaaaagaa gtgctcctga tacaacttta acattttgat tattatggaa
      601 caaatagtaa actcccggaa aaaaatggtt atggcctggg cgctggctat catcatcaca
      661 ctggcagcag gctggtatca gcgtgttaca ggtccttcac acccgctaaa agtgaatgct
      721 ggcatcaatg gcaagtctta tgttttttcg ctggcccgaa gtggcagctc gtcggataac
      781 gaaatacagg tgccggttac cgacaaagat gttaagggat atgtaattta caggagatac
      841 ccaaccaacg aaaactggga caccctggcg atgcagccgg ttaaagaggg tttgtcggcc
      901 tttttgcctg tacagccacc cgcagggaag ctggaatatt ttttgatgtt tcgcaccacc
      961 gggcaacccg atatgctcct gcaacagcaa cccacaatca tcaggtttaa aggggatgta
     1021 cccttatgga tactgctgcc acacatattg tttatttttt tcgggatgtt gttttccaac
     1081 ctggccggat tgttggcggc ttttaaattc aacagggcac gcatatattc tctgattacc
     1141 tttatcttga ttatgctggg cggcatgata ctgggcccgg tggtacaaaa atatgccttc
     1201 ggcgaattct ggaccggcgt gccctggggc tgggacctca ccgacaacaa aaccctgctg
     1261 gcttttttgg cctggctggc agccttgttt tttaaccgta acaaattaag gccgggatat
     1321 atcattgcgg cggctctcgt aactttgatt atcttttcga ttccccacag catgtttggc
     1381 tcagagttaa accccgccac aggaactatt acgcaaggat aagatgtttt acggatagaa
     1441 aaatttgact aaagttgccg caccccgtta tctcgatgcc agtcttttaa tgaattcata
     1501 gagaagtctc acgcctactc cgcttccacc tgttccacgg taagaatctt ttttgtcgtt
     1561 gaatgccggc ccggcaatgt caagatgtat aaaagggtac tttgcaaaat gtgccaggaa
     1621 ttttcctgcg gtaatggctc ctccataagg cccgcctgta tttttgatgt cggcaatact
     1681 tgatttgatg agttcgtcat attcttccca gaaaggaaat tccacaattc gttcaaaagc
     1741 gctgtcaccc gccgactgta attttttgaa atgtttttgt gcctgttgat gcatgcctac
     1801 catgccatat tttcctatgg ccgaatgtgc cgagcccgtg agcgtggcta tggtaatgac
     1861 cagcgaggga tggaattttt ttgcaaagat catcccgtcg gcaagtatca gccggccttc
     1921 cgcatcgctg ttgagcactt caacggtagt gccatcggaa tattggatga tgtcaccggg
     1981 caccagcgca tttcccgcgg gccggttgtc ggtaagcggc accagaccta tcacatgcac
     2041 cggcagtttt gttttggcaa cggcataaag cgccgaaacc accgccgctc cgccggccat
     2101 atcgtttttc atgttttcca tgtggacgga ggtctttaaa tttatacccc cggtatcgta
     2161 caataggcct ttgcccacca gcactagagg cttgccgttt acaggatgcg ttggttccca
     2221 ttccataatg agaaatgccg ggggatctat gctgcctctg ttgacggcaa gcaggccgtt
     2281 catcctgtag gctgctatct ttgctttgct aagcacctcg accctggctc cgctgttctt
     2341 caacaaatcc atggcttgtg ccgaaaatac ctgcgaattc atgtggcaca ccggctcatt
     2401 taccatgtcg cggcatatgt ggacagcttc agttagtaca ttcaggcgct gcaaatgttt
     2461 ttcaccaaaa agtttggaac atactttcaa agtttttaat ttcagttctt cttccttgac
     2521 attcgacctg tattttatga actgataggc cgaaagcgcc atcccttcaa caaaagcctc
     2581 aatgtgtgct tcctgtaacc cctgcggata cagggtgagt gtttccagat gatgttcttc
     2641 tgccagggta agcattccat gaccggcctt gcgcatctct tctgacacaa ggtgaggggc
     2701 ttttttgccg gtagaaagca aataaaaaaa caggtaatgt ccaagccggt cgatggtgat
     2761 aatccctttt ttggtttttt taattcgggc cttcagataa gcctgttctg ccttgttgag
     2821 ccggttggcg gcagttttca gcagctgagt ttccgtaggg caaataatga tgatatggct
     2881 gttttccgaa atttctttaa caagttgtat cctgagtgtc atgtggcttt tgtttaatga
     2941 ttagctattt tttgccggct tagcccactg aaaaatcgta aaattatcag cgggttgatt
     3001 tattaaaagt catacaaaaa tagtttttcc tttcaaatgc gtaattttgt gtacatgaag
     3061 gcgataaaaa ttttagacaa ggcgttaaaa actgatgcta tttccggtgc agaggcgcta
     3121 tatatttatg aaaaggcagc cttgccggat ttgatttttg ttgcaaacga aatcagaaaa
     3181 aaaatgcatc ccggcaatac ggtgggctgg attatcgacc gaaacgtcaa tattgccaat
     3241 gtttgtgttt cacagtgttt gttttgtaat ttttgcagaa agaaaaatca acccgatgct
     3301 tatatcactt cgctggacca ataccggcaa aaaattgacg aactttttgc ccttggcggc
     3361 aatcagctgt tgctgcaggg aggtatgcat cctgacctgg gactggattt ttatgtggat
     3421 ttgttcagca ccctgaaatc tttttatcca ttgttgaagc tgcatgccct tgggccgccc
     3481 gagatagttt ttattgcagg taaagaaaaa aaatcctttc gggaggtgct ggaaaaactg
     3541 gttgaggccg gccttgacag ccttccaggt gcaggcgccg aaattttgtc cgacagggta
     3601 cgaaagctta tttcaccggc caagtgttct tcaaaagaat ggcttgatgt gatgcgcgaa
     3661 gcccataaat taaaccttcc cacttcggct accatgatgt tcggacatgt ggaaaacata
     3721 caggaacgca tagaacatct gataaaaatt cgtcaggtac aggacgaaaa gccgccggct
     3781 gcctatggct ttctttcctt tattccctgg cctttccagc aaagcggaac ccgcctgatt
     3841 acgaagtttc atataacaga gatggttagt cacaacgatt atctccggtt tattgctatc
     3901 agcaggatag tactgaacaa catacccaat atacagcctt cgtggctcac ggtgggggtg
     3961 ccgctggcgc agctttgtct gcatgccggg ggcaatgact ttggttcttt gatgatagaa
     4021 gaacatgtgg tggctcaagc cggcgtcagc cggaggctcg caagtagaga gatgttgcag
     4081 gccatcaccg gagccggttt taaacctcgc ctcagaaatc agaaatttga gtatttttga
     4141 catgaaaaag attatttttt atattcacga atcccaaaaa aaattaaaat atgaaaaaat
     4201 ttgctgtact tcttgccggc tgcggctcac tggatggagc agaaattcat gaatcagtac
     4261 tgacactgc
//
LOCUS       DAOSFP010000196         4253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18783_length_4253_cov_3.691758, whole genome shotgun sequence.
ACCESSION   DAOSFP010000196 DAOSFP010000000
VERSION     DAOSFP010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18783_length_4253_cov_3.691758"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLI16_06625"
     CDS             <1..217
                     /locus_tag="PLI16_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOH84272.1"
                     /translation="SFFGPFYSGYNVIALDPDYKYALIAGKNLKYLWILSRETTIPED
                     IKQNYLKTAEGLGYKIADLVWVEHDKK"
     gene            complement(319..789)
                     /locus_tag="PLI16_06630"
     CDS             complement(319..789)
                     /locus_tag="PLI16_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84273.1"
                     /translation="MKVKITLTLFIAAVIMVSSCGSVSPTAAPSATSAEYVAGNTFGS
                     ALKSLYSQYKSQGKIVLNNQATILNLATLATSGAAIKGLGTNTSNYKNFATGVIAGST
                     NLINNNTVNNVISSVNNLNLSSVVNSLNNNTITPATTSSVTNSLVSILGMMGNK"
     gene            1043..1954
                     /gene="gldA"
                     /locus_tag="PLI16_06635"
     CDS             1043..1954
                     /gene="gldA"
                     /locus_tag="PLI16_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOH84274.1"
                     /translation="MSINVHDITKKYGKQDALHKVSFEIKSGEIVGLLGPNGAGKSTL
                     MKILTGFIPPTSGEARVCGFDVREQSLEVRKKVGYLAEQNPLYLEMYVKEFLLFVAGL
                     HTLGKEKHTRVRQMIEITGLEAEQNKKIGALSKGYRQRVGLAQALMHNPEVLILDEPT
                     TGLDPNQILEIRGLIKKIGQEKTVMLSTHIMQEVEAVCNRAIIINQGIIVADDLTANM
                     YQYLKKRNTIKVEFNANIDSEYLKKIPGVLKLTHKKANVWEVESDEDVDVRSEIFKFA
                     VKNNIEVLSMHKELVSMEDVFHQLTKK"
     gene            2108..3448
                     /gene="metK"
                     /locus_tag="PLI16_06640"
     CDS             2108..3448
                     /gene="metK"
                     /locus_tag="PLI16_06640"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOH84275.1"
                     /translation="MGYLFTSESVSEGHPDKVADQISDALLDEFLRFDPESKVACETL
                     VTTGLAVCSGEVKTTGYVDIQKVVREVIKKIGYTKAAYNFDATSCGVISTIHGQSPDI
                     NQGVVRQKKEEQGAGDQGMMFGYACKDMDNYMPMPIELAHILLQELAAIRREGKKMKY
                     LRPDSKSQVTIEYDDDGKPVRIDTIVISTQHDDFIPSKSNSEKDIKAAEKAMQDQIAT
                     DIRNILVPRVVKTLPKRVQNLFQSNFKLFVNPTGKFIIGGPHGDTGLTGRKIIVDTYG
                     GKGAHGGGAFSGKDSSKVDRSAAYAARHIAKNLVAAGVADEVLIQVAYAIGVAQPVGV
                     YVDTHGTAKVKDAKGKIMTDGEIAKKVEKIFDLRPYAIIERFSLKNPIFQETTTYGHF
                     GRTNRFEEVEVYYQNGSTKSKKVNGEVRHFKKVEFFAWEKLDYVDKIKKEFKLK"
     gene            complement(3519..>4253)
                     /locus_tag="PLI16_06645"
     CDS             complement(3519..>4253)
                     /locus_tag="PLI16_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84276.1"
                     /translation="MKKTLLFTLGLLLVFLSGNAQNLVISTSGHNLVNGDTVTVSGPS
                     TNTLHALVDVTNNAATSLAVVCRRKVVQSLPGSSNTICWGGQCWPSNVSQTPDSTVID
                     AGTTTSEFMGDFSPTGAEGTAIIRYTFFDMFNTFDSVCFVAKFISTGNSAVHELSNSL
                     SVSGIYPNPSDKAGFIEYRLQESGSTAKVIFNDMIGNEVRVIPLQEKEGKIRIATNDM
                     PEGVYFYSFVINGKVHYTKKLVVAHN"
BASE COUNT         1314 a          911 c          940 g         1088 t
ORIGIN      
        1 gtcttttttc ggacccttct actcagggta taatgtgatt gccttagacc ccgattacaa
       61 atatgccctc attgccggca agaatttaaa atacctgtgg atactttcgc gcgaaaccac
      121 tatcccggaa gacatcaaac aaaactatct gaaaaccgcg gaaggtctgg gctacaagat
      181 agccgacctg gtgtgggtgg aacatgataa aaaataaaaa tacctgtctg ccttggcctt
      241 cagacagaca gtgtctcgtt aagactatta agaagaaaaa aaatcccgcg gtagactaaa
      301 tagtctccgt ggaggtcttt atttgtttcc catcatcccc aggatgctga ccagcgaatt
      361 ggtaaccgat gaagtggtgg ccggggttat agtgttgttg tttaaagaat tcacaaccga
      421 tgacaaattc aaattattta ccgaagagat gacattgttc accgtgttgt tattaatcag
      481 gttggttgat ccggcaataa cacccgtggc aaaattttta tagttggagg tgttggtccc
      541 caggcctttg attgctgcac cgctggtagc cagggtggca agattcagga tggtggcctg
      601 gttgtttaga acaatcttac cctgtgattt atattgacta tagagcgact tcaaagcgga
      661 cccaaaggta ttgccggcca catattctgc agatgttgcc gaaggtgctg cggtgggcga
      721 aacactgccg caggaagaaa ccattattac agcggcaata aacagtgtaa gagtgatttt
      781 tactttcatt ttaaattttt atttgattta tgcaaaatca attaatattt tgtaattgac
      841 aaaaaatttc atagagatca gggttttcat tatctttttt cggcccggag ggtgaaaaat
      901 tcgcaagaat ttatcaggct aaggcatttg gggggtaacc taaagaaaat gaaagaatat
      961 gataccgtca tgccttataa atttgtaatt ttgaaccgcc aaatattttc aaacaacaga
     1021 aaaacaccct atttgcaggc ctatgtctat caacgttcac gatatcacca aaaaatacgg
     1081 aaaacaggat gcccttcaca aggtttcttt tgaaataaaa agcggagaaa ttgtcggcct
     1141 gctgggccct aacggtgcag gcaagtccac cctgatgaaa atcctgacgg ggtttatccc
     1201 gccaacatcc ggagaagcaa gagtatgtgg gtttgatgtc agggaacaat cgctggaggt
     1261 gcgcaaaaaa gtgggctacc tggctgagca aaaccctttg tacctcgaga tgtacgttaa
     1321 agaatttcta ctttttgtgg ctggcctgca cacactcgga aaagaaaaac atacccgcgt
     1381 caggcaaatg atcgaaatca cagggctgga ggcagaacaa aacaaaaaaa tcggcgccct
     1441 ttccaagggc tatcgtcagc gggtgggcct ggcccaggca cttatgcaca atcctgaggt
     1501 actcatcctc gacgaaccca ccaccggcct ggacccaaat cagatacttg aaatcagagg
     1561 cctgatcaaa aaaatcggac aagaaaaaac ggtgatgctt tccacccata tcatgcagga
     1621 agtagaggct gtgtgcaacc gggcaatcat catcaaccag ggaatcatcg ttgccgacga
     1681 ccttaccgct aatatgtatc aatacctgaa aaaacgcaac accatcaagg ttgaattcaa
     1741 tgcaaacatc gacagcgaat acctgaaaaa aattccggga gttctgaagc tgactcataa
     1801 gaaagctaat gtatgggaag tggaatctga cgaagatgtg gatgtgcgaa gcgaaatttt
     1861 caaatttgcc gtaaaaaaca atatcgaagt gctctctatg cataaagagc ttgttagcat
     1921 ggaagatgtt tttcatcagc tcaccaaaaa ataaattttg ttttttaaaa aagtagtatc
     1981 tttgcataca aaagtggaaa gatttggatt gcttgcaacc acggaaaaaa atgctaaaac
     2041 atgtttatcc cgacaataca atccaagctt ttctgattaa tttattaact aactaaaaaa
     2101 aattgttatg ggatatttat ttacatccga gtccgtttct gaaggacacc cggataaagt
     2161 tgccgatcag atttcagacg ccttacttga tgaatttctt cgtttcgacc ccgaatcgaa
     2221 agttgcctgc gaaacccttg ttaccacagg cttggccgtg tgcagcggcg aggtaaaaac
     2281 tactggctac gttgatattc aaaaagtggt gcgcgaggtc ataaaaaaaa ttggttatac
     2341 caaggccgca tacaacttcg atgctacttc ctgcggcgta atatctacta tccacggaca
     2401 atcccccgac atcaaccagg gcgttgtccg ccagaaaaaa gaagaacagg gggccggcga
     2461 ccagggcatg atgtttggct atgcctgcaa agacatggac aactacatgc cgatgcctat
     2521 cgaactggcg catatcctgc tccaggaact ggctgccatc cgccgcgaag gaaaaaaaat
     2581 gaaatacctt cggcctgatt ccaaatcgca ggttaccatc gaatatgatg acgatggcaa
     2641 acccgtccgc atcgatacca ttgttatttc tacacagcac gatgatttta ttccatcaaa
     2701 atctaatagc gaaaaagata tcaaggctgc cgaaaaagct atgcaagatc agattgccac
     2761 ggatattaga aatattctgg ttccccgcgt ggttaaaaca ttacccaaac gtgtgcagaa
     2821 tttgtttcaa agcaacttta agctttttgt taaccctacc ggaaaattta tcatcggcgg
     2881 gcctcacgga gataccggat tgaccggaag aaaaattatt gtggatacct atggcggcaa
     2941 gggcgcgcat ggcggtggcg ctttctcggg aaaagatagc tccaaagttg accgttcagc
     3001 agcctatgcg gctcgtcata ttgctaagaa cctggttgct gccggcgtgg ccgacgaagt
     3061 acttattcag gtggcttatg ctattggcgt ggcacaaccc gttggcgttt atgtggatac
     3121 ccacggcaca gccaaagtta aagatgccaa aggaaagata atgacggacg gagaaattgc
     3181 caaaaaagtg gaaaaaatat ttgaccttcg gccttatgcc atcatcgaac gctttagcct
     3241 gaaaaaccct attttccagg aaaccactac ctatggacat tttggaagaa caaaccgctt
     3301 tgaagaagta gaagtttatt atcagaacgg ttctaccaaa tccaaaaaag ttaacggcga
     3361 agtcaggcac ttcaaaaaag tagaattctt tgcttgggaa aaactcgact atgttgataa
     3421 gataaaaaaa gagttcaagc ttaaataagt atttctctta aaataaaaaa gccgcaacct
     3481 cttgaattgc ggcttttttt tatgcaacaa ccgaagaatt agttgtgcgc aaccaccagt
     3541 tttttggtgt aatgtacttt gccattgatg acaaaagaat aaaagtaaac tccttcgggc
     3601 atatcgttgg tggcaatgcg tatttttcct tccttttcct gcaatggtat aacccttact
     3661 tcgttgccaa tcatatcgtt gaaaattact tttgcagtac tgcctgattc ttgcagccga
     3721 tactcaataa aacctgcttt atctgacgga ttgggataaa taccactgac agacagcgaa
     3781 tttgacaact catgcactgc tgaattgccg gtacttataa acttggcaac gaagcatacc
     3841 gaatcaaaag tgttgaacat gtcgaaaaag gtataacgga ttatggcagt tccttcggct
     3901 cctgttgggc tgaaatcacc cataaactcc gaagtggttg taccggcatc aattaccgtg
     3961 gaatcggggg tctgcgacac attcgacggc cagcactggc caccccagca tattgtattg
     4021 gaggaaccgg gtaatgattg cacaactttc cgtcggcaaa ccactgcaag cgatgttgca
     4081 gcattattag taacatcaac caaagcatgc aaggtattag tcgaagggcc gctcaccgtt
     4141 accgtatcac cgttgacaag gttgtgtccg ctggtggaaa tgaccaggtt ttgagcgttt
     4201 cccgataaaa aaaccaggag cagtccgagg gtaaataata gtgttttttt cat
//
LOCUS       DAOSFP010000197         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18789_length_4252_cov_3.750774, whole genome shotgun sequence.
ACCESSION   DAOSFP010000197 DAOSFP010000000
VERSION     DAOSFP010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18789_length_4252_cov_3.750774"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /gene="dxs"
                     /locus_tag="PLI16_06650"
     CDS             <1..1502
                     /gene="dxs"
                     /locus_tag="PLI16_06650"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOH84277.1"
                     /translation="GMAVASVMKGEKDRHHIALIGDGSMTGGMAFEALNHTGVAKSNL
                     LVVLNDNGIAIDKNVGALKEYLTNITTSPIYNKLKLNIWNIITGKTLRKLINKLLGAI
                     KSAIVRQSNLFESLGFRYFGPVDGHDVVRLVKLFNYIKDIRGPKILHCITVKGKGFTW
                     AEKDQTRFHAPGMFDPDTGINVERPCGNKRTRYQVVFGKTLVKLAGQNPKIVGVTPAM
                     LTGCSMNMMMAKFPERTFDVGIAEQHAVTFSAGMAVQGMIPYCNIYSSFLQRAYDQII
                     HDVALQKLHVVFCIDRGGLVGEDGATHHGAFDLAYLRCIPGMIVSAPMNEEELRNLLY
                     TAQYVEQPFAIRYPRCTGVMDDWETPFQQIEIGKGRKILEGQDIAFISIGHVGNVVAD
                     AINRLHAENIFPALYDIRFLKPLDESLLHEVFRQHKKIITVEDGTVVGGLGTAVTEFM
                     NQHHYEAEIVKLGVPDRFVEQGTLDQLYAECGYDENSIFETAKKLFKTK"
     gene            1580..3319
                     /locus_tag="PLI16_06655"
     CDS             1580..3319
                     /locus_tag="PLI16_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOH84278.1"
                     /translation="MANFANLFRKKSIDDIIRHADSMSHDTQRLKKNLNVVDLTALGI
                     AAIIGAGIFSTIGNAAANGGPAVSLLFVFTALACGLSGLCYARFASVIPISGSAYTYA
                     YASFGELIAWIIGWDLIMEYAVGNIAVAISWSDYFVGMCKGINIHIPSFLAIDYLSAS
                     RGYHAVVEQLSSGAALESMTSGMRDAYDAWTTAPEVFGIKIIADIPAFCIVVFISTLV
                     YIGIRESKIASNLMVALKISILLLFLGVGIFYVNPENWSPFAPNGVSGVLKGVAAVFF
                     AYIGFDAISTTAEECKRPQKDLPRAILLSLLISTVLYILISLVLTGMAPYYELAVGDP
                     LAYVFARINLSGFSGLIAVSAVISMASVLLVFQLGQPRIWMSMSRDGLLPKRFSKLHP
                     KYKTPAFSSVVAGILVAIPTLFLNLTEVTDLTSIGTLFAFVLVSGGVLIMDNPARKKF
                     AANTKGFKIPYLNSRYLLPLLWIGVLLAVYLLNKTFFENFLSLDMYTEWHIMMEQIPL
                     LIFIVVALVLSVFAVVRQFSLIPVLGLLTNFYLMTELGITNWLRFLIWLVVGLVLYFL
                     YGYHHSRLTKTET"
     gene            3436..3756
                     /locus_tag="PLI16_06660"
     CDS             3436..3756
                     /locus_tag="PLI16_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84279.1"
                     /translation="MKKLMQRKIKIEYVLNLLFAAFMVMLIPTIHSEPGEVYQNNDSI
                     FAAAGDTIASDSLNTADFNEAVIDSVINFGKKFLGLRYKAHGQSPAGFDCSGYVSYFW
                     QVWI"
     gene            3725..4018
                     /locus_tag="PLI16_06665"
     CDS             3725..4018
                     /locus_tag="PLI16_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOH84280.1"
                     /translation="MCPIFGKYGYKFPHSSSAMSTLGEKVDLKEARKGDFIFFKGRSR
                     KSSSVGHVALIIKADSGQVTMMHSTCMSGVIIEKYNSSPYYTSRYMGCKRHRF"
     gene            complement(4041..>4252)
                     /locus_tag="PLI16_06670"
     CDS             complement(4041..>4252)
                     /locus_tag="PLI16_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84281.1"
                     /translation="TPVEIHRANLTLNALVVPPGKNEITFSYRPLPVITARYVSLGAL
                     MFVLGFIVLLIVKHKALPKELNPPK"
BASE COUNT         1081 a          915 c         1037 g         1219 t
ORIGIN      
        1 taggtatggc ggtagcctcg gtgatgaaag gcgaaaaaga caggcaccat attgcactga
       61 taggcgacgg ctcgatgacc ggaggcatgg cttttgaagc cctgaaccat accggcgtgg
      121 caaaatccaa cctgctggta gtgctgaacg ataacggtat tgccatcgat aaaaatgtag
      181 gggcattaaa ggaatatctg accaacatta ccacttcacc tatttacaat aaactgaagc
      241 ttaatatctg gaatataatt acaggcaaaa ccctccgtaa gctgataaac aagttgctcg
      301 gtgccatcaa atcggccatt gtcaggcaga gcaacctgtt cgaatcgctg ggttttcgct
      361 atttcggccc cgtggacgga catgatgtgg tgcggctggt aaagcttttc aactatataa
      421 aagatataag gggccccaaa atattgcatt gcattaccgt aaaaggtaag ggttttactt
      481 gggccgaaaa ggatcagaca cgtttccatg cccccggaat gtttgacccc gataccggca
      541 tcaatgtgga aagaccctgc ggcaataaac ggacacgcta tcaggttgtt ttcgggaaaa
      601 cgctggtgaa actagccggg caaaacccga aaattgtcgg cgtaacccca gccatgctta
      661 ccggatgttc tatgaatatg atgatggcaa agttcccgga acgtactttt gatgtgggta
      721 ttgctgaaca gcatgccgtt actttttcgg cagggatggc tgtgcagggg atgatacctt
      781 actgtaatat ttattcaagt tttttgcaac gcgcttacga ccaaatcata cacgatgtag
      841 ccctgcaaaa acttcatgtg gtgttttgta tcgacagggg cggtctggtg ggcgaagacg
      901 gcgccaccca tcatggcgct ttcgaccttg cctacctccg gtgcattccc ggaatgattg
      961 tttcggcacc tatgaacgaa gaagagttac gcaacctttt atatacagct caatatgttg
     1021 aacagccttt tgcaatccgc tatccgcggt gcacgggcgt tatggatgac tgggaaacgc
     1081 cttttcagca aattgaaata ggcaagggac gaaaaatcct ggaagggcag gatatcgcgt
     1141 ttatcagcat agggcatgtg ggtaatgtgg tggctgatgc tatcaacagg ctgcatgccg
     1201 aaaatatttt tccggcactg tatgatatcc gctttttaaa gcctcttgac gaatcgctgt
     1261 tgcatgaagt tttccggcaa cataaaaaaa tcatcactgt agaggatggc accgtagtgg
     1321 gcggtctggg tacggcggtt accgagttta tgaatcagca ccattatgaa gccgaaattg
     1381 tgaagctggg ggttcccgat cgttttgtag agcaggggac actggatcag ctttatgcgg
     1441 aatgtggcta cgacgaaaac agtatttttg aaacagcaaa aaagttgttc aaaacaaaat
     1501 aatttgattt tttcccggat cggtttactt ttgtgtcggt atcttgtcag ctacccggtt
     1561 aattcggtaa atcaaatcaa tggccaattt tgccaacctc ttccgtaaaa aatccataga
     1621 tgatattatt cgtcatgccg acagtatgtc gcatgatacc cagcggctga agaaaaacct
     1681 gaatgtggtg gatcttacgg cactcggtat tgccgctatc atcggggcgg gcattttcag
     1741 caccataggc aacgctgcag ccaacggagg accggctgtg tcgctgctgt ttgtttttac
     1801 ggccctggct tgcggcttgt cgggcttatg ttatgcccgt tttgcctccg ttattcccat
     1861 aagtggcagc gcttacacgt acgcctacgc gagcttcggt gagcttatag cctggatcat
     1921 aggctgggac cttatcatgg aatatgccgt aggaaacatt gcggtggcca tttcctggtc
     1981 cgactacttt gttggcatgt gcaagggaat taatatccac attccgtctt ttcttgccat
     2041 cgattacctc agtgcctccc gcggctatca tgctgtggtg gaacaacttt cttcaggggc
     2101 cgcccttgaa tccatgacct cggggatgcg tgatgcgtat gatgcctgga ccacggcacc
     2161 ggaagttttt ggaataaaaa ttattgccga catccccgcg ttttgtatcg tggtgtttat
     2221 ttccacatta gtatatattg gcatacgtga atcaaaaatt gcgagcaacc tgatggttgc
     2281 attaaaaatt tctatcttat tgctgtttct tggcgtgggg attttttatg tgaaccctga
     2341 aaactggagt ccttttgctc ccaatggtgt gtcgggggtg ctgaaaggtg tggcagcggt
     2401 gttttttgct tatataggtt ttgacgctat ttcaaccaca gccgaagagt gcaaaaggcc
     2461 tcaaaaagac ctgccgcggg ccatactgtt atcgctgctg atttctactg tgctgtacat
     2521 attaatcagc ctggtactta ccggtatggc tccttattat gagctggctg tgggcgaccc
     2581 gctggcttat gtgtttgcac ggatcaacct gtcgggtttt tcggggctta ttgcagtcag
     2641 cgcggtaatt tctatggcca gtgtgctgct ggtttttcaa ttggggcagc cccgcatctg
     2701 gatgagcatg agccgcgacg gcctattgcc aaaaagattc tcaaaattgc atcccaaata
     2761 caaaacaccg gctttttctt cggtagtagc cggaatactt gtagccatcc ccaccttgtt
     2821 tctgaacctc accgaagtta ccgaccttac cagtatcgga acgctctttg cctttgttct
     2881 cgtgtcggga ggcgtgctga taatggataa ccctgcacgc aaaaagtttg cggcaaacac
     2941 aaaaggattt aaaatacctt atttgaattc caggtatttg cttccgctgt tgtggatagg
     3001 cgtgttgctg gccgtttacc tgctgaataa aacttttttt gaaaactttt tatcactcga
     3061 catgtacacc gaatggcata tcatgatgga gcagatacct ttgttgatct ttatcgtggt
     3121 ggccctggtg ctgagtgttt ttgccgtggt gcggcaattt tcccttatac cggtgctggg
     3181 attgcttaca aatttttatc tcatgactga attgggtatt actaactggc ttcgtttttt
     3241 gatttggctg gtggtgggac tggttttgta ttttctatac ggataccacc atagccgtct
     3301 cacaaaaaca gaaacataaa aaaaatttgg tcggtaaaaa acttttgtat atctttgtcc
     3361 tgcaacactt tgtatgttgt tcctgtttga ggctgtaatt catggtttca caggtgataa
     3421 ggaagatatt ccttaatgaa aaagttgatg caacggaaaa taaaaataga atatgtgctg
     3481 aatttgctgt ttgcggcctt tatggttatg cttataccca ctattcacag tgagccgggc
     3541 gaagtttacc agaacaacga ttctattttc gctgctgcgg gcgataccat tgcgtccgat
     3601 tccctgaata ccgccgattt taacgaagcg gtgatagaca gcgtgataaa tttcgggaaa
     3661 aaatttctgg gattaaggta caaagcccac ggacaatcac cggcaggctt cgactgttcc
     3721 ggctatgtgt cctatttttg gcaagtatgg atataagttc cctcattctt cttcagccat
     3781 gtcaactttg ggcgaaaaag tggatttaaa agaagcccgc aagggagatt ttatcttttt
     3841 caaaggcaga agcagaaaat cctcaagtgt tgggcacgtg gcgctgatta ttaaagccga
     3901 ttcgggacag gtaactatga tgcattctac ctgcatgagc ggggtgataa tcgagaaata
     3961 caattccagt ccttattata cctcgcggta tatgggatgt aagagacacc ggttttagat
     4021 ccggaaattg cttaacaaac ttatttcggt ggatttagtt cttttggcaa ggccttatgt
     4081 tttactatta ataaaacgat aaagcccaac acaaacataa gggcccccaa cgacacatac
     4141 cgtgctgtta tcaccggcaa aggcctgtag ctgaaggtta tttcattttt tccgggaggt
     4201 acaaccagag cattcaacgt tagattcgct ctgtggattt caacaggagt at
//
LOCUS       DAOSFP010000198         4243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18849_length_4243_cov_3.679322, whole genome shotgun sequence.
ACCESSION   DAOSFP010000198 DAOSFP010000000
VERSION     DAOSFP010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18849_length_4243_cov_3.679322"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1208
                     /locus_tag="PLI16_06675"
     CDS             228..1208
                     /locus_tag="PLI16_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOH84282.1"
                     /translation="MLTIGVLGAGHLGKIHIKCLKQIETINILGFYDQDPAVAAQVEA
                     EYGVRAFTSVEELIAAVDVVDIVTPTLSHFDCASRAMKSAKHVFIEKPLVTTLEEAKV
                     LISIAGEARVKVQVGHVERFNPAFIAAFPFIQQPMFIETHRLAQFNPRGTDVPVILDL
                     MIHDIDIVMSLVKSNIKKINASGVSIVSETPDIANARLEFDNGCVANLTASRISMKNM
                     RKTRIFQADAYISVDFLKQYSEIIRLKNIEDENSIGPLDMIIDLGEGKSKKQILFEKP
                     EIVPINAIKTELETFFHSIENNTETPVSIIDGYKALDIAYKVIEKINIPD"
     gene            1272..2153
                     /locus_tag="PLI16_06680"
     CDS             1272..2153
                     /locus_tag="PLI16_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84283.1"
                     /translation="MGKKYAAGFRIIFMVVAGYAMLLSFPSCKKTDLVPSYIHIDKID
                     LNTTYEFDGTNSSKITDAWVYIDGDPQGIYELPATFPVLAKGQHTVMVRAGIKLNGIA
                     MSRAYYSFYQPWETAVELTAEQTDTLHPVVTYYENKIRWKEDFETAGMTLLKFTNSDT
                     NFIQTQDSALVFEGASSGVAYMDASAPYLLSVSDQIYEIPQNQSPVFLELNYKTSAPL
                     AIGLFAVLNTGLNNRIDVMVINPNTTWNKIYINLTPAANVSSNVTGYKVFFESYKPEN
                     QASAEVLLDNIKLLYTE"
     gene            2159..3574
                     /locus_tag="PLI16_06685"
     CDS             2159..3574
                     /locus_tag="PLI16_06685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOH84284.1"
                     /translation="MNKKLQVIKYVVSDVLSAASAWVLFFIFRKYHINHDVFNQPEMI
                     FGDKNLYIGLLFIIFFWIMLYTIIGTYHKIFRKSRLKELGQTFTISIIGMVVIFFLFI
                     LDDQVVSYKNYYLYFGIIFGLHFTFTYIGRLILSSQTAYKVHHKIIGFNTLLIGSNGN
                     ATAIYNEIENQWKSSGNKFVGFVDVHNGDHHPKEAFLPHLGSYKNLKNIILEYGIEEV
                     IIAIDKTERSFVEQIISLLEDTHVVIKIIPDLKDFLLGSVKMTSIFHAPLIQISPELM
                     PAWQCYLKRFIDVAGSLFAMTLLMPVYLLVALGVKLSSRGPVFYSHERIGLHGKPFTM
                     FKFRSMYVDAEKNGPQLSSKNDQRITPFGRFMRKVRLDEIPQFYNVLKGDMSIVGPRP
                     ERLYYINQIVQKAPHYKLLHKVKPGITSWGQVKYGYAETVDQMIDRLKFDVLYIENMS
                     LAVDFKILIYTALIILQGRGK"
     gene            complement(3575..4162)
                     /locus_tag="PLI16_06690"
     CDS             complement(3575..4162)
                     /locus_tag="PLI16_06690"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOH84285.1"
                     /translation="MKKLVILILFVLAFTAQDIYSQDTNSVVTRAIVINGDTLPVLAL
                     EEVKIFGPVVFASKYEALRFSKLVYHVKKVYPYAKIVTVKVQEFNYLISKADSKREKK
                     RLMKQAEDELKAQFEDDVKNMTSTQGEILIKLIDRETGSSSFDIIKEFRGGIVATFYQ
                     TFGRFFGYNLKSTYDPFGADKDIEQIMVMIENGEL"
BASE COUNT         1240 a          955 c          874 g         1174 t
ORIGIN      
        1 aatagcatcc ataatacgtt catccgtaat accctttgct ctgacggttt ctaccaactg
       61 ttttcgtagt cctttatgtt tataagagtc gatcattaat acttatatta ttattaatca
      121 gacatttaat actcgccatg tttggtcttt cgcctgttaa tttctcatgc gaaaataagc
      181 aaataccact tataaaaaat ttaattttgc gaaaaatacc aacccacatg ttaacaatag
      241 gtgtattagg agccgggcat cttggaaaaa tccacatcaa atgcctgaaa cagattgaaa
      301 ccatcaatat tcttggtttt tacgaccagg atcctgccgt tgccgcacag gttgaggcag
      361 aatacggtgt cagggcattt acttcggtgg aagaacttat tgccgccgta gatgtggtag
      421 atattgtaac gccgaccctg tcgcattttg attgcgcttc gcgggccatg aagtcggcca
      481 aacatgtgtt tatcgagaag cctctggtta ccacgctgga ggaagccaaa gtgcttatct
      541 ccatagccgg cgaggccagg gtaaaggtgc aggttggcca cgtggaacgg ttcaatccag
      601 cctttattgc ggcatttcct tttatccagc agcccatgtt tattgaaacc caccgcctgg
      661 cacaatttaa cccgcgcgga accgatgtgc ccgtgatact cgacctgatg atacacgaca
      721 tagacatcgt tatgagcctg gtaaaatcaa acatcaaaaa aatcaacgcc agcggggtgt
      781 caatagttag tgaaactccc gatattgcca atgcacgtct ggaattcgac aatggttgtg
      841 tggccaacct gacagcaagc cgtatctcga tgaaaaacat gcgtaaaacg cgcatatttc
      901 aggcggatgc ctatatatcc gttgatttcc tgaaacaata ttccgaaata atccggctca
      961 aaaatataga ggacgagaac agcataggtc ctttagacat gatcatcgat ctcggcgaag
     1021 gcaaaagcaa aaaacaaata ttgtttgaaa agcccgaaat tgtacccatc aacgccatca
     1081 aaaccgaact ggaaacattt ttccactcga ttgaaaacaa tacggaaacc cccgtcagta
     1141 tcattgacgg gtacaaggcg ctggacattg catacaaagt catcgaaaaa atcaatattc
     1201 cggattaaat tttcctgaga tacaataatt ttattttact accgttaacc caataaattt
     1261 cattaccgat aatggggaaa aaatacgctg ccggatttcg cataattttt atggttgttg
     1321 ccgggtatgc catgctcctc tccttcccct cgtgtaaaaa aacagacctg gtcccttcgt
     1381 atatccacat tgataaaata gatctcaaca ccacttacga atttgacggc acaaactcca
     1441 gcaaaataac cgatgcctgg gtatatattg acggtgatcc gcaaggtatt tatgaactgc
     1501 cggccacttt tccggtgctg gccaaaggac aacacaccgt tatggtcagg gcgggaataa
     1561 aacttaacgg catagccatg tcgagggcat actattcctt ttatcagccc tgggaaaccg
     1621 ctgtggaact gactgccgaa cagaccgaca ccctgcatcc cgtggttacc tattatgaga
     1681 acaaaatacg atggaaagaa gattttgaaa cggccggtat gacactgctg aaatttacca
     1741 acagcgatac taattttata caaacacagg acagcgccct ggtatttgaa ggcgccagtt
     1801 cgggggtcgc atatatggac gcctccgccc cttatctgtt aagtgtttcg gatcagatat
     1861 atgaaattcc gcaaaaccag tcgccggtgt ttcttgaact caattacaaa accagcgccc
     1921 ccctggccat aggccttttt gccgttttaa acaccggctt gaacaaccgt atagatgtga
     1981 tggtaattaa ccctaatact acctggaata aaatttatat aaatctcacg cctgctgcca
     2041 atgtatcatc gaatgtgaca gggtataaag tgtttttcga aagttacaaa cccgaaaatc
     2101 aggcatcggc tgaggttttg ctggataaca taaaattgct ttacaccgaa tgaacgtaat
     2161 gaataaaaaa ctacaggtaa ttaaatatgt cgtttcagat gtcctatcgg ctgcctcagc
     2221 ctgggtattg ttttttattt ttcggaaata ccatattaat cacgatgttt tcaaccagcc
     2281 cgaaatgatt ttcggggata agaaccttta catcggactg ctttttatta tctttttctg
     2341 gataatgtta tatactatca ttggcactta tcacaagatt ttcagaaaat cacgtttaaa
     2401 agaactcggg caaactttca ccatctctat cattggcatg gtggtgattt ttttcctttt
     2461 catactcgac gaccaggtgg tttcctacaa aaactattac ctgtatttcg gcattatttt
     2521 tggcctgcat tttactttta cctacatcgg gcgcctgata ttatcgtcgc agaccgccta
     2581 caaggtgcac cataagatca tcgggtttaa cacgctgctg ataggcagca acggcaatgc
     2641 caccgcgata tataatgaaa tagagaatca atggaagtca tcgggcaaca aatttgttgg
     2701 ttttgtggat gtgcacaatg gcgaccacca tcccaaggag gcatttttac cccacctggg
     2761 cagctataaa aacctgaaaa acatcatcct tgaatatggt atagaagagg ttatcatcgc
     2821 catagataaa actgaacgtt cgtttgtaga acaaattatt tcgctgctgg aagacaccca
     2881 tgtggtaata aaaatcattc ccgacctgaa agactttttg ctgggcagcg taaaaatgac
     2941 ctctatcttt catgctccgc tgatacagat ttcccccgaa ctaatgcccg cctggcagtg
     3001 ttacctcaaa cgatttatcg atgtggcggg ttcgctgttt gcaatgacat tgctgatgcc
     3061 ggtgtatctg ctggtagccc tgggcgttaa actgtcctcc aggggaccgg tgttttattc
     3121 gcatgaacgc ataggcctcc acggcaaacc gttcaccatg tttaagttca ggtcgatgta
     3181 tgttgatgcc gaaaaaaacg gcccgcagct atcaagcaaa aacgaccaaa gaatcactcc
     3241 ttttggccgc tttatgagaa aagtgcgtct ggatgaaatc ccccagtttt ataatgtgct
     3301 gaaaggcgat atgtcgatag taggcccgcg ccctgaacgg ctttactaca tcaatcagat
     3361 tgttcaaaaa gccccgcatt acaagctgtt gcacaaggta aagcccggta tcacttcgtg
     3421 ggggcaggta aaatacggtt atgcagaaac ggttgaccag atgatagacc gtttaaaatt
     3481 tgacgtgttg tatatagaaa acatgtcgct ggctgtagat tttaagatac tgatttatac
     3541 agctctcatc attttgcagg gacggggaaa ataattacaa ctcgccgttt tcaatcatca
     3601 ccataatttg ctcaatatct ttatcagcac cgaaaggatc gtaggtggat ttgaggttgt
     3661 agccaaaaaa acgtccaaag gtctgataaa aggtagccac gataccaccc cggaattcct
     3721 taatgatatc aaacgaagaa gagcctgttt cacggtcgat aagtttgatg agtatctcgc
     3781 cctgtgtgga cgtcatattt tttacatcat cttcaaactg ggccttcagc tcatcctcgg
     3841 cctgcttcat caggcgtttt ttctctcttt tgctgtctgc tttgctgata aggtaattga
     3901 actcctgcac cttgaccgta acaattttag cataaggata aaccttcttg acgtggtaca
     3961 ccaatttgga aaaacgcagg gcttcgtatt tgctggcaaa gactaccggg ccaaagattt
     4021 tcacttcttc gagggcgagc accggcagcg tatcgccgtt gatgactatg gcacgggtta
     4081 ccacactgtt ggtgtcctgc gaataaatgt cctgagcagt aaaagcaaga acaaacaata
     4141 tcagaattac tagttttttc atggctgcaa tatacagtat ttactgacaa tttttatgcc
     4201 agtcatacgg aattaaaaaa cacgccttat acatcgtagt tta
//
LOCUS       DAOSFP010000199         4236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18902_length_4236_cov_3.206171, whole genome shotgun sequence.
ACCESSION   DAOSFP010000199 DAOSFP010000000
VERSION     DAOSFP010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18902_length_4236_cov_3.206171"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLI16_06695"
     CDS             complement(<1..1430)
                     /locus_tag="PLI16_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84286.1"
                     /translation="MSFFNLTTEYSLWLAIPCLLLGITYAMLLYFRGQKYDFKKSTLR
                     TLFVLRTLAVFLIAFLLLSPLVMTRSRTTEKPLIILAQDNSQSPVFCKDSANIRTRYS
                     DELRQLSSSLSDDYDVKTFTFGEKVTEGTAYTYDEKQTDFSLLFDEIQARYANRNIGA
                     VILASDGLYNKGSNPLYLTGTMKYPFYTIALGDTNIQKDLIISRVNYNRISYLGNIFP
                     LETVLKADKCAGQKVTLTITKGRETLFAKTIDINSARYLETLQFQLEAKEVGMQHYRV
                     SVSTLEGEISLINNTQDVFVEVLDGREKILLLAGAPHPDVAALKQSIETSKIYQVDEF
                     VADKFNKSIKEYNLAIVHQIPLKGKNYQALLNSLTENKIPVLYILGESSDMAAFNNLK
                     TGLGIRQAAGKYDEAAPTVANDFALFTVSEELKKVSADFPALSVPFGEYLKSSSANVL
                     MYQKIGSVQTQKPLVMFNTASEPKTG"
     gene            complement(1481..2029)
                     /locus_tag="PLI16_06700"
     CDS             complement(1481..2029)
                     /locus_tag="PLI16_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84287.1"
                     /translation="MDKELTVGTRVDHERYGEGVVSKVNISNYEIIFTRGGKMEFSKS
                     NKEIEVLEYYESQAGSQAAPVDLDAFEQLMIHFLDKYGAFQEVVAMGEKWEGGKLILQ
                     PGKAELKPKEIPIEAFFHKIVMLRDRLRVLEQNINSHPQLSDQDKVNMQQYITRIYGS
                     LTTFNILFDDKEDYFVGTGGGN"
     gene            complement(2032..2685)
                     /locus_tag="PLI16_06705"
     CDS             complement(2032..2685)
                     /locus_tag="PLI16_06705"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOH84288.1"
                     /translation="MNNLPIKGNGTIIKKEIQLKEFEKIELDGIFKVFLKQGDKAKLE
                     VETDENLLDCIGANVRNSKLTIKLKKPVHSTKGINIYLTFNKINNIEVSGHSYVVSSD
                     RLNFVRLDMAVSGTSKMIAEVNAVSLKMEASDNSSVKFSGNVFSADIAASGIANIDAA
                     NMNIDDLKLSVSGNAASMVNVINSFDVNASGNAQVLYKGTPDMKESVSGNATVNKKY"
     gene            complement(2768..3052)
                     /locus_tag="PLI16_06710"
     CDS             complement(2768..3052)
                     /locus_tag="PLI16_06710"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOH84289.1"
                     /translation="MQQEKPLKEVIRQLIEYYRLDRKLNEATLIEKWEEIVGKMIAIH
                     TIKLYVDKRVLYVELDSSIVRNELNLIKSEIVKRLNSYFNKPFIDKIILK"
     gene            complement(3055..4167)
                     /gene="recF"
                     /locus_tag="PLI16_06715"
     CDS             complement(3055..4167)
                     /gene="recF"
                     /locus_tag="PLI16_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810122.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOH84290.1"
                     /translation="MYLQKIALINFKNYPELDLGFSEKINCFVGNNGVGKTNLLDAIH
                     YLSFCKSYFNVIDTQNILHEADFFTIQGVWVKNPDNPDTIFCGQKRNQHKVFKLNKKE
                     YSRLADHIGLYPLVMVSPHDSDMIYSGSEERRRFLDSFISQFDHVYLDDLINYNKALF
                     QRNKLLKIFAESNSFDADALEIWDTQLVALGTKIHAKRTSFILDFLPYFNYYFELISG
                     KKESVSMTYDSQLNSAHLNDLLKSSLAKDRIVQYTTCGIHKDDLIFNIGDFPLRKFGS
                     QGQQKSYTIAIKLAQFEYIKIMKGYKPVVLFDDIFDKLDDERVEHLVKLATDNNFGQV
                     FITDTQKERIVNTMKKINSPGKVFMISNNNAKEINL"
BASE COUNT         1086 a          869 c          938 g         1343 t
ORIGIN      
        1 attccggttt taggttccga ggcggtatta aacatcacca ggggtttttg tgtttgcaca
       61 ctgccgattt tctggtacat caacacgtta gcggaactac tttttaagta ctcaccaaaa
      121 ggtaccgaca gtgcaggaaa atcggcactg acctttttca gttcttccga aacggtaaac
      181 aaggcaaagt cgttggccac ggtgggtgca gcttcgtcgt attttccggc agcctgcctt
      241 atgcccagac ctgttttcag gttgttgaag gcggccatat cagacgattc gccgaggatg
      301 tataataccg gtattttgtt ttctgtaaga ctattcaaaa gggcctgata gttcttccct
      361 ttcagcggta tctgatgcac tattgcaaga ttgtattctt ttatagattt gttgaactta
      421 tcggcaacaa attcgtccac ctggtagatt ttactggttt cgatgctttg tttcagggcc
      481 gctacgtcgg gatgcggggc tccggccagc aacaaaattt tttcacggcc atccagcact
      541 tccacaaaga catcctgtgt attgttgata agactaatct cgccctccag tgttgaaaca
      601 cttacacggt aatgctgcat gccaacctct ttggcttcca gctgaaattg cagggtttcg
      661 aggtagcggg cgctgttgat atcaatagtt ttggcaaaca aggtttcgcg gcctttggtg
      721 atggtcagtg tgactttttg tccggcacat ttgtcggcct tgagcactgt ttccaatggg
      781 aagatattgc ccaggtaact gatgcggtta taattgacac gtgaaataat gagatctttc
      841 tgaatgttgg tatcgccgag cgcaatggta tagaaagggt atttcatagt tccggtcaga
      901 tacaaagggt tgctgccttt gttgtacaag ccgtcagaag ccagtatcac cgcgcctata
      961 ttacggttgg catagcgtgc ctgtatctcg tcaaaaagga gcgaaaaatc ggtctgtttt
     1021 tcatcataag tatatgctgt gccttcggtg actttttcgc cgaatgtaaa tgtcttaaca
     1081 tcataatcat cgctcagact ggaactcagt tgacggagtt catcggaata ccgggttcgg
     1141 atgttggccg aatctttgca aaaaacgggt gactgtgagt tgtcctgcgc aaggatgatc
     1201 aggggttttt cggttgtgcg gctgcgtgtc attaccaggg gcgacaacaa caaaaaagca
     1261 ataagaaaaa ccgccagggt acgcaaaaca aaaagggtac gaagcgtgct ttttttgaaa
     1321 tcatattttt gtccccggaa atacaacagc atggcatagg taatacccag cagcaggcag
     1381 ggaatggcca gccataaaga atattcggtg gtgaggttaa aaaatgacaa ggattttttt
     1441 tgtgaacaaa gataaggata ataatacttt tgaaagacga ttagttgccc cctcccgtgc
     1501 ccacaaaata atcctcctta tcgtcaaaca aaatattgaa ggtggtcagg ctgccataaa
     1561 tacgggtaat gtattgctgc atgtttacct tgtcctggtc ggacagttgc gggtggctgt
     1621 tgatattctg ttcgagcacg cgcaggcggt cgcgcaacat gacaatcttg tgaaaaaaag
     1681 cttcgatggg tatttctttg ggttttaatt ctgctttgcc gggttgtaag atcagtttgc
     1741 ctccctccca tttttcgccc atggcaacca cttcctggaa ggcgccgtat ttgtcgagaa
     1801 aatgaatcat cagctgttca aacgcatcca gatcaacggg tgcagcctgg ctgccggctt
     1861 ggctttcata atattccagc acttcaattt ctttattgct ttttgaaaat tccattttgc
     1921 ctccacgcgt aaaaataatc tcgtaattag agatgtttac tttgctgacg actccttcgc
     1981 cgtatcgctc gtgatccact ctggtaccta ctgtaagttc tttgtccatg attagtattt
     2041 tttgtttact gttgcattcc ccgaaacaga ttctttcatg tccggtgttc ctttatatag
     2101 cacctgggca ttacctgagg catttacatc gaaagagttg ataacattta ccatcgaagc
     2161 ggcattgccc gaaaccgaaa gtttcaggtc gtcgatattc atgttggcgg catcgatgtt
     2221 ggctatgccg gaagccgcaa tatcggctga aaaaacattg ccggagaatt tcacagagct
     2281 gttgtcgctg gcttccatct tcaggctaac ggcgttgacc tcggcaatca ttttgctggt
     2341 gccggaaacg gccatgtcca ggcgaacgaa gttcagcctg tcggatgaga caacatagga
     2401 atgacctgag acttcaatat tatttatctt attgaatgtc aagtatatat ttattccttt
     2461 tgtgctatgc accggttttt tcagttttat cgtcagtttg ctgttgcgga cgtttgcccc
     2521 gatgcagtcc aataagtttt cgtcggtttc cacttcaagt tttgctttgt cgccctgttt
     2581 cagaaagacc ttaaaaatcc cgtccagttc gattttttca aattctttaa gttgaatttc
     2641 ttttttaata atagtgccat tgcctttgat cggcaagttg ttcatacatg aagtaatact
     2701 acaaataaga cttgtaagta aaataataac agttaagtaa gtaatttttt tcatcagagt
     2761 tcaggaatta ttttaagata attttatcga taaaaggttt gttaaaatag ctgttcaggc
     2821 gttttacgat ttcggattta atcaggttca gttcgttacg gactattgaa gaatcgagtt
     2881 cgacataaag cactcgttta tcaacgtaaa gtttgatggt atgtatggca atcatcttac
     2941 cgacaatttc ctcccatttt tctataaggg tggcttcatt gagttttcta tcgagccggt
     3001 agtactcaat aagctgcctg atgacctctt tcaatggttt ttcctgttgc ataatcagag
     3061 gttaatttct tttgcattat tgttgcttat cataaacacc tttccaggag aattaatttt
     3121 tttcatagtg tttacgatcc tttctttttg ggtatcagta ataaaaacct gtccgaaatt
     3181 attatcagtg gcaagtttca ccaggtgttc caccctttca tcatcgagtt tatcgaagat
     3241 atcatcaaaa agcactaccg gtttataacc tttcatgatt ttgatatatt caaactgggc
     3301 gagtttaatg gcaatggtgt atgatttttg ctgaccctgg gagccaaact ttcgcagcgg
     3361 aaaatcgcca atattaaata tcaggtcgtc tttgtgtatg ccgcaggtgg tgtattgaac
     3421 tatcctgtct ttggcaaggc ttgatttcag caggtcgttc aggtgagcac tgttgagctg
     3481 cgagtcgtaa gtcatcgaaa cgctctcttt ttttccgcta atcagttcga aatagtagtt
     3541 aaagtagggc aaaaaatcca ggataaaaga tgttctttta gcatgtatct tggttcccag
     3601 ggctaccaat tgtgtatccc atatttctaa ggcatcggca tcgaaggagt tgctttcggc
     3661 aaagatcttc agcagtttat tgcgctgaaa aagggctttg ttgtagttta tcaggtcgtc
     3721 gaggtacaca tggtcgaact ggctgataaa actgtcgagg aacctcctgc gttcttcgct
     3781 gccgctatag atcatgtcgg agtcgtgcgg cgacaccatc accagggggt atagccctat
     3841 atggtcggcc agccggctgt actctttttt gttcagctta aaaactttat gttgattgcg
     3901 tttttgtccg cagaaaatgg tgtcggggtt gtccggattt tttacccaaa caccctgaat
     3961 ggtaaaaaaa tcagcctcgt gcaggatgtt ttgcgtgtca atgacattga aataactctt
     4021 gcaaaacgaa aggtaatgaa ttgcatcaag caggttcgtc ttccccacgc cgttatttcc
     4081 aacaaaacag ttgatttttt ccgaaaaacc cagatcgagt tccggataat ttttaaagtt
     4141 gattaatgca attttttgca ggtacataac ggattagttt ggtgaaaacc ctaaaaagaa
     4201 ctccttttca cttttttttt taccataaag catatt
//
LOCUS       DAOSFP010000200         4227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_18949_length_4227_cov_3.265101, whole genome shotgun sequence.
ACCESSION   DAOSFP010000200 DAOSFP010000000
VERSION     DAOSFP010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18949_length_4227_cov_3.265101"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..638)
                     /locus_tag="PLI16_06720"
     CDS             complement(27..638)
                     /locus_tag="PLI16_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84291.1"
                     /translation="MENLFSKVVSAVFQPVFMPVYTYLLIYNIDSYISFALNPSVKIL
                     LFLMILFSTVILPVLVFVIFKRNYVISSFHMKTNEERNYPYLVTALIYFGLYLLLSKT
                     ALPPIYGVFFMAATVLSLVLLFINLRFKISAHMAGIGGVSGLLAGIAFRWNLELTLPV
                     IIAIACAGLVGYARLKLNAHKPSEVYFGFLAGVSGFLLFTLLL"
     gene            complement(643..2151)
                     /gene="rpoN"
                     /locus_tag="PLI16_06725"
     CDS             complement(643..2151)
                     /gene="rpoN"
                     /locus_tag="PLI16_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOH84292.1"
                     /translation="MLNQKLQQKLQQKLSPQQIQLMKLLQLPTIAFEQRIKEELENPA
                     LEDANESDMKDEPQEQEENSLETDETETEGSEELEKEIEEVEKDPEEVSFEDFLEEDD
                     TPAYKLSAYEYNPDEDDHKEIPFSVGQTFHESLITQLGLRKLDERQKQIATFIIGNLD
                     DSGYLQRDLAAMVDDIAFTLSINTTEKELEELLKIIQEFEPYGVGARNLQECLVLQLN
                     KKEELTPAVALAKTILLKFFTEFTKKHYDKIEKRLDINSEQLKQAMHEILQLNPKPGN
                     SMIDNAKPNQYITTDFIITNNDGELELTLNAKNTPELRINKAYLDMIDTMSSNKKHRT
                     GQDKELAIFVKQKLDSARWFIDMIKQRHDTLYKTMKAIMDFQREYFLDGDETSLKPMI
                     LKDIAEKVNLDISTISRVVNSKYVQTHFGTFLLKSFFSESLQTESGEEVSTREVKKIL
                     TDCIGSESKKKPLTDEQLTKILKDKGYNIARRTVAKYREQLDIPVARLRKEL"
     gene            complement(2246..3646)
                     /gene="asnS"
                     /locus_tag="PLI16_06730"
     CDS             complement(2246..3646)
                     /gene="asnS"
                     /locus_tag="PLI16_06730"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOH84293.1"
                     /translation="MKEQYPSYKIKDLLKLTDYGKIVNLKGWVRSKRGNKQLAFIAMN
                     DGSVIHNIQVVAELEKFDEETIKLISTGACLSVNGSLVESPGQGQQVEIQAINIEVYG
                     TADAAIYPLQKKGHTLEFLREIAHLRPRTNTFSAVFRIRHAMSFAIHKFFNDRGFFYL
                     HTPLITGSDAEGAGALFQVTTLDMEKPPRKEDGSIDYAQDFFGKRTSLTVSGQLEGEL
                     GAMALGLIYTFGPTFRAENSYTPRHLAEFWMIEPEMAFYDITDNMNLAEDMLKYLITY
                     ALEHCIDDLEFLQKMYDNELISRLNFVLANPFERLAYTEAVEILMASGKKFEYPVGWG
                     TDLQSEHERYLVENHFKKPVILTDYPKEIKAFYMKQNPDGKTVRAMDVLFPKIGEIIG
                     GSQREEKYDLLLNRIREMGISEKEMEWYLDTRKFGTAPHSGFGLGFERLMLFITGMAN
                     IRDVIPFPRTPQNAEF"
     gene            3829..>4227
                     /locus_tag="PLI16_06735"
     CDS             3829..>4227
                     /locus_tag="PLI16_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HOH84294.1"
                     /translation="MIIKQGEILSGINSVEDLRKLKVEQLPALCEDLRQFIIDSTSLN
                     PGHLGANLGTIELTVALHYVFNTPYDKLVFDVGHQAYTHKILTGRKDLFHTNRMYKGL
                     SGYPRMSESEFDAFGVGHSSTSISAALGMAV"
BASE COUNT         1064 a          879 c          974 g         1310 t
ORIGIN      
        1 agggttgact atttcgccct attgatttac agcagtagcg taaacaacaa aaaacccgaa
       61 acgcctgcaa gaaatccgaa atatacttcg gagggcttgt gggcgtttag ttttaatcgt
      121 gcatatccca ccaggcctgc acaggcaata gcaattatta ccggcagggt aagttccagg
      181 ttccagcgaa aggcaattcc ggcaagcagg ccggaaacac caccaatgcc tgccatgtgg
      241 gcgctgattt taaagcgcag gtttataaac aacaacacca gcgacaatac tgtggcagcc
      301 ataaaaaata ccccgtatat ggggggcagg gcggtttttg ataataaaag gtacaggcca
      361 aaatatatca gggctgttac cagatacgga taattccttt cctcattggt tttcatgtga
      421 aaactgctga taacataatt tcttttaaaa atgacaaaaa ccaatacagg taagataact
      481 gtcgaaaata aaatcattaa aaataagaga atctttactg aagggttcag tgcgaacgaa
      541 atataactgt cgatgttata gataaggagg tatgtgtaca caggcatgaa cacgggttga
      601 aaaaccgccg aaaccacttt tgaaaaaagg ttttccataa aattaaagct cttttctgag
      661 ccgggccacg ggtatatcga gttgttcgcg gtatttggcc actgtacggc gggcgatgtt
      721 atagccttta tctttgagta ttttagtaag ctgctcgtcg gtaaggggct ttttcttgct
      781 ttctgacccg atgcagtctg taagtatttt tttgacttcc cgggtggaga cttcttcgcc
      841 tgattcggtt tgcagcgatt cggagaaaaa actttttagc agaaaagtgc cgaaatgagt
      901 ctgcacatat ttgctgttta ctacgcgtga gatggttgag atatcgaggt ttaccttttc
      961 tgcaatatcc tttaaaatca tgggtttcag gctggtctcg tcgccgtcaa ggaaatattc
     1021 cctttgaaag tccatgatgg ctttcatggt cttgtaaagg gtgtcgtggc gttgctttat
     1081 catgtcgatg aaccagcggg ccgagtctag tttttgtttt acaaaaatgg cgagttcttt
     1141 gtcctgtccc gttctgtgtt ttttgttcga tgacatggtg tcaatcatgt ctaagtatgc
     1201 cttgttgatg cgcagttcgg gggtgttttt tgcattcagg gtgagttcca gttccccgtc
     1261 gttgttggta ataatgaagt cggtggtaat gtattggttg ggtttggcat tgtcaatcat
     1321 cgagtttccg ggcttggggt tgagctgcag gatctcatgc atagcctgtt tcaattgttc
     1381 gctgttgatg tcgaggcgtt tttcgatttt gtcgtagtgt ttttttgtaa actcggtaaa
     1441 aaactttaaa aggattgtct ttgccagtgc caccgcgggt gtaagttctt cttttttgtt
     1501 aagctgtaat acgaggcatt cctggaggtt tcgtgctccc acgccatagg gctcaaattc
     1561 ctgtatgatt tttaacagct cttccagttc tttttcggta gtatttatgc tgagggtaaa
     1621 ggcaatatca tcaaccatgg cggccaggtc tctttgcaga tatcccgaat catcgaggtt
     1681 gccgataatg aacgtggcga tttgtttttg gcgttcgtca agcttacgca gacctagctg
     1741 ggtgattagc gattcatgaa aggtttgccc taccgaaaaa ggtatttcct tgtgatcgtc
     1801 ttcgtcgggg ttgtattcgt aagcactgag cttgtaggca ggggtgtcgt cttcttccag
     1861 aaaatcttca aaagatactt cttccgggtc tttttctact tcttctattt ctttttccag
     1921 ctcttcgctg ccttctgttt ctgtttcatc tgtctcgagg ctgttttctt cctgttcctg
     1981 cggctcatct ttcatgtccg actcgttggc gtcttccaac gcagggtttt ccagctcttc
     2041 tttgatacgt tgctcaaaag ctatggtggg cagctgtaac aatttcatca actgtatctg
     2101 ttgcggcgag agtttttgtt gcagcttctg ctgtagtttc tggttgagca tagccaaact
     2161 tttttatttt gttacgaata taatattttt tattggtttg atcaaaaaat cttaaacacc
     2221 acgttaatga aagaacattc caatattaaa attcggcatt ttgcggagtt cgcgggaaag
     2281 ggataacatc tctaatgttg gccatcccgg tgatgaacag catcagccgt tcaaatccaa
     2341 ggccgaagcc gctgtgtggt gcggtgccga attttcttgt gtcgagatac cattccattt
     2401 ctttttcaga aatgcccatt tcgcggatgc ggttcagcaa gaggtcgtat ttttcttcgc
     2461 gctgtgagcc gccgataatt tcgccaatct tggggaaaag cacatccatg gcgcgtacgg
     2521 ttttgccatc gggattttgt ttcatgtaaa aggccttgat ttctttggga taatcggtaa
     2581 gtattactgg ttttttaaaa tgattttcta ccaggtatct ttcatgttcc gactgcagat
     2641 cagtgcccca gccgacaggg tattcaaatt ttttccccga tgccatcaga atttctactg
     2701 cttcggtgta ggcaagccgt tcaaacgggt tggccaatac aaaattcagg cgtgagataa
     2761 gctcattatc gtacattttc tgtagaaatt ccaggtcgtc aatacaatgc tccagggcat
     2821 aggtaatcag gtatttcagc atgtcttccg ccaggttcat attgtcggtt atatcataaa
     2881 aggccatctc gggttctatc atccagaatt cggcaagatg gcgcggcgta taggaatttt
     2941 cagcccggaa agtaggcccg aaagtataaa tcaacccaag cgccatggct cccagttcgc
     3001 cttcgagttg acccgaaacg gtaagactgg tccttttgcc aaaaaagtcc tgtgcataat
     3061 caatgctgcc atcttctttg cgaggcggtt tttccatgtc gagcgtggta acctgaaaca
     3121 gcgcaccggc gccctcggca tcggaacccg tgataagcgg ggtgtgcaga tagaaaaaac
     3181 cgcggtcatt aaaaaacttg tgaatggcaa acgacatggc atggcggatc cgaaaaacag
     3241 cgctgaatgt attggttcgg ggccgcaaat gtgctatctc ccgaagaaat tccagggtgt
     3301 gccctttttt ctgtaaagga tagatggcag catcggccgt gccgtaaacc tcaatgttta
     3361 tggcttgtat ctccacctgc tgaccttgtc ccggagattc taccaggctg ccgttcaccg
     3421 aaagacaagc tccggtgctg atgagtttta tagtttcttc gtcaaacttc tccagttcgg
     3481 ccaccacctg tatgttgtga atcaccgaac catcattcat ggcgataaaa gccagttgtt
     3541 tgttccctct tttggatcgc acccaacctt tgaggttgac aatttttcca taatctgtta
     3601 gttttaacag gtctttaatt ttataggagg gatattgctc tttcataagt cattttttta
     3661 tgcaaaaaaa acaattttaa ggatgattta caactataat attttgatga cgcgctaata
     3721 atctcataat caatgtttag aggacaatac aaaatgtgag taatccgtat ccatgaaaaa
     3781 tttaataatt ttgtacaaga atttaataaa aagcgagttt gtttttaaat gataataaaa
     3841 caaggggaaa tactttcggg aattaattcc gttgaagatc ttagaaaact caaggtagaa
     3901 cagttgccgg ctttgtgcga agacctgagg cagtttatca ttgattccac gtcgttgaat
     3961 ccgggacacc tgggcgccaa cctgggtacc atagaactta cagtagcctt gcactatgtg
     4021 tttaatacgc cttacgacaa gctggttttt gatgtgggac atcaggccta tacacacaaa
     4081 atacttaccg gacgcaaaga cctttttcat accaaccgca tgtacaaagg tctcagcggg
     4141 tatccgcgca tgtccgaaag tgaatttgat gcctttggtg tcggtcattc ctccacgtct
     4201 atctctgccg cattaggtat ggcggta
//
LOCUS       DAOSFP010000201         4197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19126_length_4197_cov_4.212216, whole genome shotgun sequence.
ACCESSION   DAOSFP010000201 DAOSFP010000000
VERSION     DAOSFP010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19126_length_4197_cov_4.212216"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="PLI16_06740"
     CDS             <1..1430
                     /locus_tag="PLI16_06740"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOH84295.1"
                     /translation="QAKLLNAIKFSGRGKKGTAVGVFNAITNNMYARIEDSLGNTSKI
                     LTEPLTNYNILVLDQILKNNSKIYLVNTNVLRDKKYDDANVTLLGVTLNDKKNRYQFA
                     GFAAMSNKLHKADTGSRKITSTPGYQYSLSFAKVSGKFQFSIYRKEMNHKWDANDIGL
                     TLQNNRSTTGATIGYKIFEPFWILRDMAHSIEIYHIENFLAKRVEDFRITYSGNATTR
                     KYLTIWGSFILSPLKVNNFQDTRSPGRYFVMDRWFYASLNGSSDYRKTFALDVNLEYW
                     QEIYGDGVSYGVTLNPIIKPSDKFTFNIKSIYNGNYNDIGFVYNDTIGNIIMGKRDII
                     IVQNQFEGRYMFRNNLALSLLVRHYWSKGIYDKHYKLDYDGILITDPLLPLDKDYNFN
                     FNAFNIDLMFYWEFAPGSSLNITYKNNILKEGSQIVPSYFKNFGNIFAEKQLNSLSLK
                     VLYYIDYQQLKSFAHQRKTRKTRHD"
     gene            1423..2076
                     /locus_tag="PLI16_06745"
     CDS             1423..2076
                     /locus_tag="PLI16_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH84296.1"
                     /translation="MIKAQNIQKSYENLKVLKGINLEIEKSEIVAITGASGAGKTTLL
                     HILGTLDKPDNGTVEINGQVISGLNEKKLAAFRNKHIGFVFQFHHLLPEFTALENVCI
                     PAFIAHISKKEAEKNAREILDFLGLSSRLSHKPSQLSGGEQQRVAVARALINKPDVIL
                     ADEPSGNLDSANARELHKLFFDLRENFSQTFVIVTHNAEFAGMSDRLLHMKDGMMEE"
     gene            complement(2150..2848)
                     /locus_tag="PLI16_06750"
     CDS             complement(2150..2848)
                     /locus_tag="PLI16_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84297.1"
                     /translation="MTTLQILSVTTYKQRKSLFSLVYHIIFLSFFIPVAGYAQSDTVM
                     VKYTRDFKFNDGIFQTFQEFKNNKPSLPEFEVVKDKIYGEEGSIILRYPCPDSIGSKK
                     TCVIDECFGYSRNGVLYIYQGYPGYYYRMFMVGALSHFVAFNKYGMHDMYFSPEPLAY
                     VGTSNDFAEYLLDLETGETIHFNYRDFCKFLENHDSELFQELQNTRQKRKMIHHFLLK
                     YNEKHPVYFPVSVQ"
     gene            complement(2879..>4197)
                     /locus_tag="PLI16_06755"
     CDS             complement(2879..>4197)
                     /locus_tag="PLI16_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84298.1"
                     /translation="GISNIFAMDTATKKVFQVTSALYGVADPFVDNSGKELVYADYTA
                     DGYRPVKASLDSSKWMLFEKNDSFSDKNSYPTETSGAQVIDFYDKEKSNFPVKRYSKI
                     LNLFNIHSWGPVSVNADNTTVKPGVELLSQNLLSTMIVSAGYEHEWSKPVSQVYAKLS
                     YRGWYPRIDLQFAYRFNKEDTITWDVMFLQAGIKIPWKFNMGKYYISIQPEVDFNFYR
                     LFPQKNYPAENFSGFYQAMEYKLFGYHVLKRSLRDINPRWGQVLEFSYKHTPFDGKQF
                     GDITALQGILFFPGIGRHHSLNIYAGYQRNRAKHYMFNDLISLPHGYNSAMYPKIFST
                     QTAYEMPLFYPDWSIGSLFYIKRFRGTLYYDHAWVTDRYDKRQSLNSAGLALLADFHM
                     FRFLAPLSMGVRATCRLDNKAMSYEFIYSVNFDQLMFRRGFSRTGF"
BASE COUNT         1283 a          936 c          857 g         1121 t
ORIGIN      
        1 ggcaggcaaa actgctcaac gcaataaaat tttcgggcag gggcaaaaaa ggcacggcag
       61 ttggcgtttt caatgccatt acgaacaata tgtatgcacg tattgaagac tccctgggga
      121 atacaagcaa aatcctcacg gaaccgctta ccaattacaa tattctggtg ctggaccaga
      181 tactcaaaaa caattcgaag atataccttg tgaacaccaa tgtgctccgc gataaaaaat
      241 atgacgatgc caatgtaacc ctgttgggag tgaccctgaa tgataaaaag aaccggtatc
      301 agtttgccgg ctttgccgcc atgagcaaca aactgcacaa ggccgacacc ggcagccgta
      361 aaataaccag cacacccgga tatcaatatt ctttgtcgtt cgcaaaagta agcggcaaat
      421 ttcagtttag tatctaccga aaagaaatga accataaatg ggatgccaac gatatcggcc
      481 tcacgttgca gaataaccgt agcactacag gagccactat tggatataaa atatttgagc
      541 ctttctggat actgcgggat atggcccaca gcattgaaat ttatcatatt gaaaactttc
      601 ttgccaaacg cgtggaagac tttcgcataa cctattccgg caatgccacc accagaaaat
      661 atcttaccat ctggggttcc ttcatcttaa gccctctgaa agtaaataat tttcaagaca
      721 cccgcagccc cggacgatac ttcgtaatgg accgttggtt ttatgccagc ctcaacggct
      781 cgtcggatta ccgaaaaacc tttgccctgg atgtaaacct tgaatactgg caggaaatct
      841 atggcgacgg agtatcttat ggcgtcactc ttaatcccat catcaagcca tccgacaaat
      901 ttacttttaa cataaaaagc atatacaacg gtaactacaa tgatatcggc tttgtgtata
      961 atgacaccat tggcaatatc atcatgggca aaagagatat tattatcgtg cagaatcagt
     1021 ttgaaggcag gtatatgttc aggaataatc tcgcactgag cctgctcgtc aggcattatt
     1081 ggtcaaaagg tatttatgat aaacattaca agctcgatta tgatggcata ctgattaccg
     1141 acccgctgtt accccttgac aaggactata attttaattt caatgcattt aacatcgacc
     1201 ttatgtttta ttgggaattt gctcccggca gcagcctgaa catcacttat aaaaataaca
     1261 tcctcaaaga aggcagccag attgttccat cgtatttcaa aaatttcggc aacatattcg
     1321 cggaaaaaca actaaattcg ctgtcgttaa aggtattgta ctatatcgat taccagcagc
     1381 taaaaagttt tgcacaccaa agaaaaacaa gaaaaacccg ccatgattaa agctcagaat
     1441 atacagaagt cctatgaaaa cctcaaggtg cttaagggca tcaacctgga gatagaaaaa
     1501 agcgaaattg tggccattac gggggcttca ggggccggaa agaccaccct ccttcacata
     1561 ctcggcacct tggacaagcc cgataacggc acggttgaaa tcaacgggca ggttatttcg
     1621 ggacttaacg aaaaaaaact ggctgctttc cgcaataaac acattggttt tgtgtttcag
     1681 tttcatcatc tgttgccaga atttactgcc cttgagaatg tttgcatccc ggcttttatt
     1741 gcccacattt ccaaaaaaga agccgagaag aatgccaggg agatacttga ttttcttggg
     1801 ttaagcagcc ggctgtcaca caaaccttcg caactgtcgg gaggcgaaca gcaaagggtg
     1861 gccgtagccc gcgccctgat aaacaaaccc gatgttattc ttgctgatga gccctcggga
     1921 aacctcgatt cggccaatgc cagagaactt cataaactat tcttcgacct gagggaaaat
     1981 tttagccaga cctttgtgat agtaactcac aacgctgaat ttgcgggtat gtcggaccgc
     2041 ttattgcata tgaaagacgg catgatggaa gagtagaaaa acaaaatttt tttctgtcag
     2101 ttaattgcat catggagcct gtggaaccaa aaacagcgat tttcacagat cattgaacag
     2161 ataccggaaa ataaaccggg tgtttctcgt tgtattttaa caggaaatga tgtatcattt
     2221 ttcttttctg ccttgtattc tgaagttcct gaaacagctc gctgtcgtgg ttttcaagaa
     2281 acttacaaaa atccctgtag ttaaaatgta tggtttcgcc ggtctccaga tcaagtaagt
     2341 attctgcaaa atcatttgaa gtgcccacat acgctaaagg ttccggtgaa aagtacatat
     2401 cgtgcatgcc atacttgttg aaggcaacaa aatgtgacaa tgcgcccacc atgaacatac
     2461 ggtaataata gcccggatag ccctgataga tatataaaac tccattcctg gaatacccaa
     2521 aacactcatc aatcacgcag gtttttttgc ttccaatgga gtcggggcaa ggatagcgaa
     2581 gaattatgct accttcttcg ccatatattt tgtcttttac cacctcaaat tcgggcaggg
     2641 aaggtttatt gtttttaaat tcctggaatg tctgaaatat tccgtcgttg aacttaaaat
     2701 cacgggtata ttttaccatc accgtatcgc tttgtgcata tcccgcaaca ggaataaaaa
     2761 acgacaaaaa aataatgtga tatactaaag aaaacagcga ttttctttgc ttgtatgtgg
     2821 ttaccgaaag aatttgcagc gtagtcacag ccttattttt taacaaaatt acaaaaaatc
     2881 aaaatcctgt tcgcgaaaaa ccacgcctga acatcagttg gtcaaaattt acactataaa
     2941 taaattcgta cgacatggct ttgttgtcga gacggcatgt ggcgcggact cccatgctca
     3001 ggggcgccag aaatcgaaac atatgaaaat cggccagcag agccagtccc gccgagttga
     3061 gtgattgacg cttatcatac ctgtcggtaa cccaggcatg gtcgtaatac aaagttcccc
     3121 tgaaacgctt gatataaaac aaagaaccta tgctccagtc gggatagaac aagggcattt
     3181 cgtaggcggt ttgggtggaa aatatttttg gatacatagc gctgttatac ccatgcggca
     3241 acgaaatcaa atcgttgaac atgtagtgtt ttgccctgtt gcgctggtaa ccggcataaa
     3301 tgttcaggct gtgatgccgc ccgatacccg gaaaaaaaag tatgccctgc agtgccgtaa
     3361 tgtccccaaa ttgttttccg tcaaacgggg tatgtttgta tgaaaattcc agcacctgcc
     3421 cccagcgcgg attaatatct cgcaacgacc gtttcaacac atggtatcca aaaagcttgt
     3481 attccatcgc ctgataaaaa cccgagaagt tttcggcagg gtagtttttt tgagggaata
     3541 atctgtaaaa attaaaatct acttccggct gtattgaaat ataatacttt cccatgttga
     3601 acttccacgg aatctttatg cctgcctgca aaaacatcac atcccaggtt atggtatcct
     3661 ctttgttgaa cctgtaggca aactgcagat ctatgcgggg gtaccagccg cgataactca
     3721 atttggcata aacctgcgaa acaggcttgc tccattcatg ttcatatcct gcactaacaa
     3781 tcatcgtact cagcaggttt tgggatagaa gctccactcc gggtttcacg gtagtattat
     3841 cggcattgac agacacaggc ccccaactgt gaatattgaa caggttcagg atcttggaat
     3901 atcgtttaac aggaaagtta ctcttttctt tgtcatagaa atctatcacc tgcgctccgg
     3961 atgtttcagt agggtaagaa tttttatcag aaaaactatc gtttttttca aacagcatcc
     4021 atttcgatga atcgagactt gctttcaccg gcctgtatcc atcggcagtg tagtcagcat
     4081 agacaagttc tttgccgcta ttatccacga atgggtcagc aactccatat aaagcggaag
     4141 ttacctgaaa caccttttta gttgccgtat ccatagcgaa aatgttgctt atccccg
//
LOCUS       DAOSFP010000202         4173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19279_length_4173_cov_4.020155, whole genome shotgun sequence.
ACCESSION   DAOSFP010000202 DAOSFP010000000
VERSION     DAOSFP010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19279_length_4173_cov_4.020155"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..767)
                     /gene="clpP"
                     /locus_tag="PLI16_06760"
     CDS             complement(87..767)
                     /gene="clpP"
                     /locus_tag="PLI16_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOH84299.1"
                     /translation="MDSSKEFRKYAVKHLGITSSRLDKFSAAHDMTRTIIEERQVNFR
                     EVDVFSRLMMDRIIFLGTPVDDYVANIIQAQLLFLESADAKKDIQIYINSPGGSVYAG
                     LGIYDTMQYISSDVATICTGMAASMSAVLLCAGAKGKRTGLKHSRILIHQPMGGAEGQ
                     ASDIEITAREIQKLKKELYEIIATHSGQTFQKIWKDADRDYWMTAEEALKYGMIDEVL
                     SSKNKNGK"
     gene            complement(770..2170)
                     /gene="tig"
                     /locus_tag="PLI16_06765"
     CDS             complement(770..2170)
                     /gene="tig"
                     /locus_tag="PLI16_06765"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOH84300.1"
                     /translation="MKIEQEKTGELTASIKIELEPADYQEKVDKQLKEYQRKANMPGF
                     RPGHVPFGLVKKMYGKSLLYEVVNKEYSEALYKYIDEQKINILGAPLANEEKNTPIDL
                     DKDTILTFWFDIAFAPEFTVDITDKTQVNHYKISVTDETVEKYLAEMQKRHGTFEQVE
                     MVDDNDMISGEFAELDENGNLKEGGIKNDAFIYMEQLADEEKRSKFRGLKAGEFVDVN
                     PKEIARNDFEATYFIGKKKEELENISSLFRFTVKLINRNKNAELNEEFYKKVYPGLPI
                     ENIDQMKEQIRKDAIDSYQKECERKFVNDATEIIRNNTQFDLPEAFLKRYILETQTDT
                     KLTKEKIEEDFEQHRDVIKWQLIENKIIKEHGLNVTAEEMRDFVKGYFRKVNEHEHEH
                     THEEGEAEAHHHEPDENYLNQIADNILKNEEEAKRISDKLFDDKLLAFLKSKLSVNYV
                     DVSYDEFVKIISTKKQ"
     gene            complement(2376..2457)
                     /locus_tag="PLI16_06770"
     tRNA            complement(2376..2457)
                     /locus_tag="PLI16_06770"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2421..2423),aa:Leu,seq:tag)
     gene            2610..3464
                     /locus_tag="PLI16_06775"
     CDS             2610..3464
                     /locus_tag="PLI16_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH84301.1"
                     /translation="MLFLLLAIVTSSLIFIAFKLFEKNGISNIQAITVNYLTAVIIGY
                     LSTEEIITTYVVFGKPWFYFSVGSGILLIVTFLVYAYSIQKVGIAITSVSGKMSVIIP
                     VLLGLLVFQEEATWIRIIGIVTALLAFYLTLMKNREQKAKAIFFVFPVLLFLGNGLND
                     CVLKISQELYIQNDFSMYLTIAFGVSLIAGAIIITFLLITKQQKIHYKNLIAGIILGI
                     LNWYSTYFFFAGLAQFDVSVFIPVFNASVVCIAALTGYWFFKEKLSGINIVGIALAVI
                     AIIFIAWK"
     gene            complement(3513..4127)
                     /locus_tag="PLI16_06780"
     CDS             complement(3513..4127)
                     /locus_tag="PLI16_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84302.1"
                     /translation="MKKFLVTLLITGLVFSAQVYAQQGKGSDKPPKPPKPQPVKVKTP
                     PVPKPAVGQPPKPVVPVKPGEPQTPVKPQNPQGKDKTQGEVKDKENQGKGNAYGKNKE
                     GLEGKEFGQHRAAEAKSKQEAVSNSKQSINSTTKTNEDTHAKIKEAKNKLEQNKKDKK
                     ITDAEYNKKKKELDDLEMQVNELEKKNSEVKDKLNKESSAKGKK"
BASE COUNT          999 a          899 c          821 g         1454 t
ORIGIN      
        1 aatatggcct tccagtccgg caatcagcag attgacttcg gaccgctttt tacctcaaaa
       61 agaacatttt tctgtttccc gtggcattat ttaccgtttt tatttttact gctgagcacc
      121 tcgtctatca taccatattt cagcgcttct tcggctgtca tccaatagtc gcggtccgca
      181 tccttccaga ttttctgaaa tgtttgtccc gaatgtgtgg caattatttc gtagagttcc
      241 tttttcaatt tctgaatctc gcgggctgtg atttcgatat ccgaggcttg tccttcggcg
      301 ccgcccatag gctggtggat gagtatgcgc gaatgtttca gtccggtacg ctttcctttg
      361 gcgccggcac aaagcaacac cgccgacatg gaggctgcca tgcccgtgca aatggttgct
      421 acatctgaag agatatattg catggtgtca taaataccga gtcccgcata aacagaacct
      481 ccgggagaat tgatgtaaat ctgtatatct tttttggcat cagccgattc cagaaacaaa
      541 agctgggctt gtataatgtt agccacgtag tcatcaacgg gagtgccaag aaaaataatc
      601 cggtccatca tcagcctgga aaaaacatca acttcgcgaa aatttacctg acgttcttca
      661 attatagtac gtgtcatatc gtgtgcagcc gagaatttat cgagccgcga actggtaatg
      721 ccgaggtgtt ttacggcata cttgcgaaat tctttggagg aatccatact tattgttttt
      781 tggttgaaat tatttttaca aattcatcgt acgacacatc aacataattc acgctaagtt
      841 tacttttcag gaaggccagc aatttatcat caaaaagctt atcgctgatg cgcttggctt
      901 cttcctcgtt cttcaaaata ttatctgcta tctggttcag gtagttttca tccggttcgt
      961 gatgatgtgc ctcagcttcg ccttcttcat gagtgtgttc gtgttcatgc tcattgactt
     1021 tacggaaata gccttttaca aaatcccgca tttcttccgc agtaacattg agcccgtgtt
     1081 ctttgataat tttgttttcg ataagctgcc atttgataac atcgcggtgc tgctcaaagt
     1141 cctcttcaat cttttctttg gttagtttgg tatctgtctg tgtttccaga atatatcgtt
     1201 tcaggaaagc ctcgggcaga tcgaactgtg tattgttgcg gataatttcc gtagcatcgt
     1261 tcacaaactt acgttcacat tccttttgat aactatcaat agcgtccttg cgtatctgct
     1321 ctttcatctg gtcaatgttt tcgatgggca gccctggata taccttttta taaaattctt
     1381 cattcagttc ggcgtttttg ttgcggttga taagcttaac cgtgaaacgg aacaacgaag
     1441 agatattttc aagctcttcc tttttcttgc cgataaaata ggtagcttca aaatcgttgc
     1501 gggctatctc ttttggatta acgtcaacaa attcgccggc cttgagtccg cggaatttac
     1561 ttctcttctc ttcgtcggcc agttgttcca tgtaaataaa cgcatcattt ttaattccgc
     1621 cctctttcag gttcccgttt tcatccagtt cggcaaattc acccgaaatc atatcgttat
     1681 catccaccat ttcaacctgt tcgaaggtgc catgacgttt ttgcatttcg gccaggtact
     1741 tttccacagt ttcgtcggta acgctgattt tgtaatgatt aacctgagtt ttatcggtaa
     1801 tatccacggt aaattcagga gcaaaagcaa tatcgaacca aaaagtcagg atggtatcct
     1861 tatcgagatc gatgggcgta tttttctcct catttgccag gggagcgccc agaatgttga
     1921 ttttttgttc gtcaatatat ttatataaag cttcgctata ttctttattc actacttcat
     1981 aaagcagtga ttttccgtac atttttttca ccaggccaaa gggaacgtgg ccgggcctga
     2041 acccgggcat attggcttta cgctggtatt ccttgagctg tttgtcaact ttttcctgat
     2101 aatcggccgg ttcgagttca atttttatcg aagccgtgag ttcacctgtt ttttcctgtt
     2161 ctatcttcat ctaaattctg tttttatgtt gtctgtctga aaattataat tgaagaaatc
     2221 ttttttcaat taatccttat tacgcatgcc tgcccgcctc agacagggat gtagggactt
     2281 gcgccaaaaa gcggacaacc tgtctgttgc caggcaggtt tctcgtcccg ttttcgtcaa
     2341 agaacgtaaa atttatcctg ttcatcatca ttccgtgcgg atgaagggac tcgaaccccc
     2401 acgtctttcg acaccagatc ctaagtctgg cgcggctacc aattacgcca catccgcaaa
     2461 acgaggtgca aagatacaaa aaaattaaaa tctcaaatac aagcaattaa taatattata
     2521 ttgagataaa tcgcataaat cacagcttaa actgattcat aaataattac tcaaattatt
     2581 ctacttttgc gcacttcaaa ttgggaaata tgttgttttt gttgttggcc atcgtaacct
     2641 cgagtcttat ttttattgca ttcaaattat ttgaaaaaaa cggaataagc aatatacagg
     2701 caattaccgt taactatctg acagcggtaa taatcggata tctctctacc gaagaaatca
     2761 tcacaacata cgttgttttt ggcaagccct ggttttattt ttctgttggc tccgggattt
     2821 tattgatagt aacctttttg gtttatgcat attcgattca aaaagtcggg attgccatta
     2881 ccagcgtttc gggcaaaatg tcggtaatta ttcctgtact tttgggatta ctggtatttc
     2941 aggaagaagc aacctggata aggattatcg gaattgttac cgccctgctt gctttttacc
     3001 tcacgctgat gaaaaaccgc gagcaaaaag ctaaggccat attttttgtt tttccggtgc
     3061 tgctgttttt gggcaacgga cttaacgact gcgtgttaaa gatatcacag gaactctata
     3121 tccaaaatga tttcagcatg tatttgacca tagcattcgg agtttcgcta atagccggag
     3181 ctataattat tacttttttg ttgataacca aacaacaaaa aatacattat aaaaacctca
     3241 ttgcaggaat tattctgggt atcctgaact ggtattccac ctattttttc tttgcaggac
     3301 ttgcacaatt cgatgtaagt gtgtttatac cggttttcaa tgccagtgtg gtttgcattg
     3361 ctgccctcac cggctattgg ttctttaaag aaaagctctc cggtataaat atcgttggca
     3421 tagcgctggc tgtaattgcc ataatattta tcgcctggaa ataaaaaaag gcctcccttt
     3481 cggaaagcct ttataagttc accttgacaa ggttatttct tacctttggc cgatgattct
     3541 ttgttgagct tgtctttcac ttcgctgttc ttcttttcca gttcatttac ctgcatttcc
     3601 agatcgtcga gttccttttt ctttttgtta tactcagcat cggttatctt cttgtctttt
     3661 ttgttttgtt ctaatttgtt tttcgcctct tttatcttgg cgtgcgtatc ttcgttggtt
     3721 ttggtagtac tgttgattga ctgctttgaa ttggatactg cttcctgttt tgattttgct
     3781 tctgcagccc gatgttgtcc gaattctttg ccttccaggc cttccttatt ttttccatag
     3841 gcattgcctt tgccctgatt ttctttatct tttacttcac cctgagtttt gtctttgccc
     3901 tgcgggtttt gtggttttac aggcgtttgg ggttctccgg gttttacagg aacaactggt
     3961 ttaggcggct ggccgacagc cggtttgggt acaggaggcg ttttaacttt tactggctgc
     4021 ggtttgggag gcttcggggg cttatcgctt cccttgccct gctgtgcata tacctgtgca
     4081 ctgaaaacca gtccggtaat gagcaatgta accagaaatt ttttcataat ggtaatgttt
     4141 taaatgtttt ttaataaact atcagctttc tca
//
LOCUS       DAOSFP010000203         4158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19387_length_4158_cov_2.510358, whole genome shotgun sequence.
ACCESSION   DAOSFP010000203 DAOSFP010000000
VERSION     DAOSFP010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19387_length_4158_cov_2.510358"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLI16_06785"
     CDS             <1..402
                     /locus_tag="PLI16_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HOH84303.1"
                     /translation="FASDVSVLYFPAGLCSRKHAHGIIQDLEWKKTFISYARKYQRDI
                     IPAYIDGGNSNFFYNLARLRGKLGVKANIEMFYLVDEMYKQKNKTINITFGNPIPYQL
                     FDKSHTDQQWALKVKEHVYKLESSHDAEFLK"
     gene            407..1333
                     /locus_tag="PLI16_06790"
     CDS             407..1333
                     /locus_tag="PLI16_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH84304.1"
                     /translation="MENIIAPVSKSVLEKELSDKYFFRKTNFGNNHIYIVNAHNAPNV
                     MQEIGRLREITFRDAGGGTGKSVDIDEYDICESPYEQMIVWNPADMEIVGGYRFIECA
                     KALYNPEGLPYIATTELFSFSQRFLKEFLPHTIELGRSFVQPGYQPLVNSRKGMFSLE
                     NLWDGIGGLLMEMPHIKYFFGKVTMYPHFNQRARDIIMYFLFKYFPDKDNLVKPITPL
                     EIKTDLKELEPIFNGGNYGADYKMLIQTLRELNENIPPLVKAYMNLTSTMRSFGTAIN
                     PGFGSVQETGILVTVEDIYENRKERHISSFRK"
     gene            1341..1955
                     /gene="yihA"
                     /locus_tag="PLI16_06795"
     CDS             1341..1955
                     /gene="yihA"
                     /locus_tag="PLI16_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOH84305.1"
                     /translation="MVIKSARYISSCVDLKKCPVPALPEYAFIGRSNVGKSSLINMLT
                     GVKGLAKVSASPGKTRVINHFLINEEWYLVDLPGYGFAKISKELKKEWEKMISHYLTR
                     RRNLICTFVLVDIRVPVQQKDLDFINNMGESQLPFVIVMTKADKVSKQEGIKTLSAWK
                     RALSENWNECPQMLVTSAGTGQGRDEILDFIHKNNKFFVATALK"
     gene            complement(2009..2641)
                     /locus_tag="PLI16_06800"
     CDS             complement(2009..2641)
                     /locus_tag="PLI16_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84306.1"
                     /translation="MIKPQYQPISDKHRNTIDELGRKQGLWKYYTRDKTLVMEISFQN
                     DVKHGPCIRHSSATGAVIEESNYFNGRRDGEYKRYDIKGTIISEGQYVNGRKSGKWTT
                     YYTVNGEKKSEGEYQSGKREGVWANYNSKGTLRAKGAYKYGLREGEWIYYYANGTVAE
                     TKTYIKGKAPEEVKQPKQPEPKVVKGFVMTPKKKKETPGENKKTGQELNP"
     gene            complement(3041..3619)
                     /locus_tag="PLI16_06805"
     CDS             complement(3041..3619)
                     /locus_tag="PLI16_06805"
                     /inference="COORDINATES: protein motif:HMM:NF040058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84307.1"
                     /translation="MLFKYHIKQSVLFLCIFGLLVSFTACNNKKNSENKITLTERIQY
                     PVFIKSPYDEDTDWWCENMEGPKREQFINIIVDAALSGKVKAYDYINNTPLTKSEVEV
                     IFNRYDTVLYTRPVAPFDEVDTVINTKLDRKDIHRVTFLEEWYFDEKNFIIEKKVVGV
                     APALTLYADSGEIKGYKPLFWVYMDDKYPLKK"
     gene            complement(3649..>4158)
                     /locus_tag="PLI16_06810"
     CDS             complement(3649..>4158)
                     /locus_tag="PLI16_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84308.1"
                     /translation="DEEGWSHTGDLGKLEPTGQLTITGRKKEIFKTSFGKYVNPGQIE
                     AKFSESPLIDTMIVLGENQKYAAALIVPDFNDLKSWCENKGLVYTTNPEMINHPDVKN
                     KYKKIIAHYNSFFGETEQIRRWEIMDSEWSVWSGELTPTLKIKRAYINNKYKDIISKL
                     FEGSEILQQ"
BASE COUNT         1119 a          826 c          889 g         1324 t
ORIGIN      
        1 tttgcctcgg atgtcagtgt gctgtatttc cctgccggcc tgtgttcgcg aaaacacgct
       61 catggtatta ttcaggacct ggagtggaaa aaaactttta tttcatatgc ccgtaagtat
      121 cagcgtgata tcatccccgc ctatatcgac ggcggaaatt caaatttttt ctataacctg
      181 gcacgcctca ggggcaagtt gggtgtgaaa gccaatatcg agatgtttta ccttgtggat
      241 gaaatgtaca agcaaaaaaa caaaaccatc aatattacct ttggcaaccc cattccttac
      301 cagctttttg ataagagcca caccgaccag caatgggcgc taaaggtcaa agaacatgtt
      361 tataaactgg aatcgtcaca cgatgccgaa tttttaaaat agaattatgg aaaatattat
      421 tgctcctgtg tcaaaatcag ttctggaaaa agaactttca gacaaatatt tttttcgcaa
      481 aaccaatttt ggaaataatc atatttatat cgtcaatgcg cataacgccc ccaatgtcat
      541 gcaagagatc ggtcgcctgc gtgaaattac ctttcgtgat gccggcggcg gaacgggtaa
      601 gtctgttgat atcgatgaat acgacatttg tgagagtcct tatgaacaaa tgatagtgtg
      661 gaatcctgcc gatatggaaa ttgtgggcgg ctatcgtttt attgagtgtg ccaaagcgct
      721 ttataacccc gaaggacttc catatattgc caccaccgaa ttgtttagtt tttcgcaacg
      781 ttttcttaaa gagtttttgc cacacaccat cgagctgggt cgttcttttg tgcagcctgg
      841 ttatcaacct ttggtaaaca gccggaaagg gatgttttcg ctggaaaacc tgtgggatgg
      901 cattggcggc ctgctgatgg aaatgccgca tattaaatat tttttcggca aggttaccat
      961 gtatcctcat tttaatcaac gggcgcgtga tattatcatg tattttcttt ttaagtattt
     1021 tcctgataaa gataatctgg taaagcccat caccccgctg gagataaaaa ccgacttgaa
     1081 agagctggag cctattttta acggaggaaa ttatggcgct gattataaga tgcttattca
     1141 aaccctgcgt gaacttaacg aaaatattcc tcctttagtg aaggcataca tgaacctaac
     1201 ttccaccatg cgtagtttcg gaacggctat caatcctggt tttggcagtg ttcaggaaac
     1261 gggtatcctg gttacggtgg aggatattta tgagaacaga aaggaacggc atatttcctc
     1321 attccggaaa tagtaataaa atggtgataa aatcagccag atatatttcc agttgtgttg
     1381 acctgaaaaa atgtcctgtg ccggcactac ccgaatatgc ttttattgga cggtccaatg
     1441 ttggcaaatc ttcactgatt aatatgctca cgggcgtcaa aggcttagcc aaagtttccg
     1501 cgagcccggg caagaccagg gtcatcaatc attttcttat caacgaagaa tggtacttag
     1561 tggatctacc gggatatggt tttgcaaaga tctcaaaaga gttaaaaaaa gaatgggaga
     1621 agatgatttc gcattatctt acccggcggc gaaacctgat atgtaccttt gtgctggttg
     1681 atatccgggt gccggtgcaa caaaaagatt tggatttcat aaataacatg ggcgaatcgc
     1741 aattgccttt tgtaatagta atgacaaagg cggataaggt ttcgaagcag gaaggaatta
     1801 aaacgctttc ggcatggaaa agagcacttt ctgaaaattg gaatgaatgt ccccagatgc
     1861 tagttacctc ggccggaaca ggccagggac gtgatgaaat tctggatttt atccataaga
     1921 acaacaaatt ttttgttgca acggcattga aatagatagg ttggtaattg gtataaaatc
     1981 cttcgctgca gggatttggc gttgctgtct atgggttaag ttcctgtcct gtttttttgt
     2041 tttcgcccgg ggtttctttc tttttcttgg gtgtcataac aaagccttta accacttttg
     2101 gttcgggttg ttttggctgt tttacttctt cgggagcttt tcctttgata tacgtcttgg
     2161 tttcggcaac tgtaccgttg gcataatagt aaatccattc accttctcgc aggccgtatt
     2221 tgtatgctcc tttggccctt aacgtacctt ttgagttata attagcccac actccttcgc
     2281 gtttgccgct ttggtattcc ccttcgcttt ttttctctcc gttgacggta taataggtgg
     2341 tccactttcc cgattttcgg ccgttaacat attgtccttc tgaaataatg gtgcctttga
     2401 tgtcgtatcg tttgtattct ccgtcgcgac ggccgttgaa atagtttgat tcttcaatca
     2461 ctgctcctgt ggctgaagag tgtcgtatgc agggcccgtg ttttacatca ttctgaaatg
     2521 atatttccat caccagggtt ttatcgcggg tataatactt ccacaaaccc tgtttgcgac
     2581 ccaattcgtc aatggtgtta cggtgcttat ccgaaatcgg ctggtactgt ggctttatca
     2641 atgtctcttt ttgagaaaaa agataaagag gcgccaacaa taatagcata caaagtgtat
     2701 aagtaaaatt atttttcacc ggccatcagt ttacttcaaa gatattaaaa aaatgaaaaa
     2761 aaacctaact tttttgttta tacgtataga acacaaaaaa gttttagagg ctgtctcaaa
     2821 atgcattttt tttgatattc ttcactcagt ggctctctgt gaaccgcaat agcggtctct
     2881 gtgaatctca tgtaataata aattaaagaa tcacacagag tgccacggag aacacacaga
     2941 cctgcctgcc ggcgaggcag gggcacacag aatacttttg agacagcctc taagaattta
     3001 taattgaaca gtaggatttt agcacatcat tttttgccgg ctattttttc aaaggatact
     3061 tatcgtccat atatacccag aacaatggct tgtatccttt tatttcgccg ctatcagcat
     3121 aaagggtcag ggcaggagct acaccgacaa cctttttttc aatgataaag tttttttcat
     3181 caaagtacca ctcttctaaa aaagtcaccc tgtgaatatc cttccggtcg agtttggtgt
     3241 ttattaccgt gtcaacctcg tcgaaagggg ctacgggacg cgtgtacagt actgtgtcgt
     3301 accggttgaa tatgacttct acttcacttt tagtcaacgg ggtattgttg atgtaatcat
     3361 atgcttttac tttgcccgac agggcggcat caacaatgat atttatgaac tgttcgcgtt
     3421 tggggccttc catgttttcg caccaccagt cggtatcttc gtcgtaagga cttttgataa
     3481 aaactggata ctggatacgt tcagtcaggg ttattttatt ttcagaattt tttttgttgt
     3541 tacaagccgt gaacgatact aacaatccaa aaatacataa gaataatact gattgtttta
     3601 tatgatattt aaaaagcatt tatgtagagt attgaaataa aacaaaagtc attgctgtaa
     3661 aatctcgctt ccttcaaaaa gtttgctgat gatgtctttg tatttattgt tgatataggc
     3721 gcgtttgatt ttcagggtag gagtgagttc tcctgaccag acagaccatt cggaatccat
     3781 gatttcccac cggcgtattt gttcggtttc gccaaaaaat gaattgtaat gggcaataat
     3841 tttcttgtat ttgtttttta cgtcaggatg gttgatcatt tcggggttgg tggtgtacac
     3901 caatcctttg ttttcgcacc atgatttcag gtcgttgaaa tcaggcacta tgagggcagc
     3961 agcatatttc tgattttcgc ccagcactat catagtatca atcagcggcg attcggaaaa
     4021 ttttgcttct atctggcctg ggtttacata ttttccgaac gaagttttga agatttcttt
     4081 tttacgtccg gtgatggtca gttgaccggt gggttctaac tttcccaggt cgccggtatg
     4141 agaccatcct tcttcgtc
//
LOCUS       DAOSFP010000204         4147 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19463_length_4147_cov_3.299609, whole genome shotgun sequence.
ACCESSION   DAOSFP010000204 DAOSFP010000000
VERSION     DAOSFP010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19463_length_4147_cov_3.299609"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2338
                     /locus_tag="PLI16_06815"
     CDS             <1..2338
                     /locus_tag="PLI16_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84309.1"
                     /translation="ITAHGWAQYNITGTVREKNSGQALSGASVGIVGQYLGTVSNPSG
                     EFILKNIKQGSYTLKVTYVGYSDFTKEIELDKDLSLTIELEKRTILQDEVIISATRAA
                     ENEPSTFTNVDKQEISSINFGQDLPYLLSSTPSVVVSSDAGNGIGYTSMRIRGTDISR
                     INVTMNGVPVNDAESQSVFWVDLPDFASSVDNVQIQRGVGTSTNGAAAFGASINFQTT
                     KLNPSAYADYNLSYGSFNTWKNTLSAGTGLLAGHWAVDVRLSKLNSEGYIERAASDLK
                     SMFISGGYYGEKTIVKAIITSGKEKTYQAWNGVPKDSLKTNRRYNPFTYENQTDNYQQ
                     DNYQLLASQEIGKHFLINAAAHYTYGRGYYEEFEEYSPFASYGLPDVINGIDTLTAAD
                     LVRQKWMNNHFYGATFSGTYDNKKNLKIIVGGAGNQYSGDHFGDIIRCNYLNITEHPY
                     RWHENNTKKNEFNIFTKVNLTIKKKVFLYADLQYRTVKFVLDGIDSKLRDVSQTHIFH
                     FFNPKLGVTYNINDRHSMYLALALSNREPNGNNFADADPAGPAPVRENMFNTELGYTF
                     SSKYVTASCNGYFMYYHDQLVLTGEINDVGYAIMSNVPRSYRAGIELSGAARLFKRFT
                     WELNAGFSQNKIINFTEFVDDWDNGGQIQNQLGNTNIAFSPAITAVNDFTYNFKEHFN
                     ISLISRYVSRQYIDNTSDKARSLDPFFVSDLLMEYSFKLGPIKEIRVSLMLNNIFNEK
                     YENNAWVYRYYYNGAFNTMDGYFPQAGINFMAGLKVRL"
     gene            2407..3948
                     /locus_tag="PLI16_06820"
     CDS             2407..3948
                     /locus_tag="PLI16_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH84310.1"
                     /translation="MKTSFINIRKSGILCLLFLYSIISNAQKVPEFITDSLDNYVGKA
                     LKIWNIPGVAVCVVKDGKVIINKGYGVCELGKTEKVDENTLFMIGSNTKAFTGTALAW
                     MAVENKCSLSDKVKKWVPEFSAKDPWVNDNANLTDIVCHRMGTSTFQGDFTFFDSDLN
                     SSQIIEKYGKLTPVNEFRTKWGYCNTGFLLAGRALKPISGLSWEDFMRTKIFGPLQMK
                     RSLPLTSEINNKTNKASAHTIIENVVKVIPYGNFDALAPAASICSSVEDMSHWLIALL
                     DSGNYLNKNVIPYEAILKTREPQAVIGNAYHPFNRSNYSLYGLGWELQDYEGHGLVMH
                     NGGIDGFVTSVTLVPDENLGIVVLTNTDQNYLYEALKWEILDAYLNLPYRNYSKLYNN
                     YFKRRAQRDNNEIKVWRDSATMNITLPLPLSEFTGIYQNEVYGTITIKKEAGLLKLNF
                     EHHKNLSAKLEYIGNNRFLCTYSQPLFGIKVFPFVVEGGKVTSFTLSVADFLEYTTYK
                     FTKQK"
BASE COUNT         1251 a         1004 c          890 g         1002 t
ORIGIN      
        1 tatcactgct catgggtggg cacagtacaa catcaccggc acggtccgcg aaaaaaactc
       61 cgggcaggcc ctgagcggag catctgtggg catcgtagga caatatctcg gtacggtcag
      121 caacccatcg ggagaattta tccttaaaaa cattaaacag ggttcgtaca cgctaaaggt
      181 tacttatgta gggtactccg atttcaccaa agaaatagag ctggacaaag acctttcact
      241 gacaatagaa ctggaaaaac gaaccatcct gcaggatgag gtcatcatca gcgctaccag
      301 ggccgcagaa aatgaacctt ccacctttac caacgtggat aaacaggaaa tcagcagtat
      361 aaatttcgga caggatcttc cctacctcct gagttctaca ccttcggtgg tggtgtcttc
      421 tgacgcgggc aatggaatag gctataccag catgcgtatc cggggcaccg acataagccg
      481 gatcaatgtt accatgaatg gtgttcctgt gaacgatgcc gagtcgcaga gcgtattctg
      541 ggtggacctg cccgactttg cttcttcggt agacaatgtc cagatacaac gtggtgttgg
      601 cacctctacc aatggggccg cggctttcgg ggccagcatc aattttcaga ctacaaaact
      661 aaacccctca gcctacgccg attataattt gtcatacgga tcttttaaca catggaaaaa
      721 tactttatcg gcaggaacag gcctgctcgc cgggcattgg gcagttgatg tacgcctgtc
      781 aaaactcaat tccgaagggt atattgaaag agccgcttcc gatctgaaat ccatgtttat
      841 cagtggcggg tattatggcg aaaaaaccat tgtgaaagcc ataatcacct cgggcaaaga
      901 aaaaacttac caagcatgga acggcgtccc caaagactcg ctgaaaacca accgccgcta
      961 caaccctttt acctatgaaa accaaacgga taattatcag caggacaatt accagttgct
     1021 ggcatcgcag gaaatcggaa aacattttct tattaacgct gctgcccatt atacatacgg
     1081 acggggctac tacgaagaat ttgaagagta ttcgcctttt gcttcttacg gattgcctga
     1141 tgtcatcaac ggtattgaca ccctgacagc ggctgacctg gtgcgtcaga aatggatgaa
     1201 caatcatttc tatggagcca ctttttcagg cacctacgac aacaaaaaaa atctcaaaat
     1261 aattgtcggt ggcgctggca accaatattc cggtgaccat ttcggagata tcatccggtg
     1321 caactacctc aacatcacag agcatccata ccgctggcac gaaaacaaca caaaaaagaa
     1381 cgagtttaac atcttcacta aagtaaatct caccataaag aagaaagtgt ttctttacgc
     1441 cgaccttcaa taccggaccg taaaatttgt gctcgacggc atcgattcca aacttcgcga
     1501 tgtgtcacaa acgcatattt ttcatttttt caaccccaag ctgggcgtaa catacaacat
     1561 caacgacagg cacagtatgt acctggcact ggcattatca aacagggaac ccaacggcaa
     1621 caactttgcc gatgccgatc cggcaggacc ggcgccggta cgcgaaaaca tgttcaacac
     1681 cgaattaggg tacaccttca gcagcaaata tgttaccgcc agttgcaacg ggtattttat
     1741 gtattaccac gaccagctgg tgctcaccgg cgaaatcaat gatgtggggt atgccatcat
     1801 gtcgaatgtg ccacggagtt accgcgccgg cattgagctc agcggcgccg ccaggctgtt
     1861 caaacgcttc acatgggaat tgaacgccgg cttcagccaa aacaagatta tcaatttcac
     1921 cgaatttgtt gatgactggg acaacggagg gcagatacaa aaccagcttg gtaacacaaa
     1981 catcgctttt tcgcctgcca taaccgcggt aaatgatttc acctataatt tcaaggaaca
     2041 tttcaatatt tcacttatca gcaggtatgt aagcaggcaa tacatcgata acacctccga
     2101 caaagctcgt tcgctggacc cgttctttgt gtccgatctc ctgatggaat actctttcaa
     2161 actggggccc atcaaggaaa tccgcgtatc gttaatgttg aacaacatct ttaatgaaaa
     2221 atatgaaaac aatgcctggg tgtaccgtta ttattacaac ggcgccttta acactatgga
     2281 cggatatttt ccgcaggcag gaattaactt catggccggg ctcaaagtca ggttatagtt
     2341 tgattcagga tatttttcgc ctgcccgttt cataaaaaaa ttgtaggttt gccaacaaaa
     2401 aaacctatga aaacttcatt tataaatatc aggaaaagcg gtattctttg cctgctgttt
     2461 ttgtacagca tcatttcaaa tgcgcaaaaa gttccggaat tcatcaccga cagcctcgac
     2521 aattatgttg gcaaagcgct gaaaatatgg aatattcccg gggttgccgt ttgcgtagtc
     2581 aaagacggaa aagtaattat aaacaagggt tacggcgttt gcgaacttgg caaaaccgaa
     2641 aaggtggacg agaacacgct gtttatgatt ggctccaaca ccaaggcctt taccggaacg
     2701 gcgctggcct ggatggctgt tgaaaacaag tgctctctca gcgacaaagt aaaaaagtgg
     2761 gttcccgaat tttcggccaa agacccctgg gtgaacgaca atgccaatct taccgacatc
     2821 gtatgccacc gcatgggaac ctccactttt cagggtgatt ttacattttt cgactccgat
     2881 ctgaacagca gccagatcat cgaaaaatac ggcaaactaa cgcccgtcaa tgaattccgc
     2941 accaaatggg gatattgcaa cacaggattt ttactggctg gacgggcatt gaaacctatc
     3001 agcggcctga gctgggaaga ttttatgcgg acaaaaatat ttggccccct gcaaatgaaa
     3061 cgaagcctgc cgctcacctc cgaaataaac aacaaaacca acaaagcctc ggcacacacc
     3121 atcattgaaa acgtagtaaa agtaatccct tacggcaact tcgacgccct ggcccctgca
     3181 gccagcattt gctcttcggt ggaagatatg agccattggc tgatagcatt actcgatagc
     3241 ggaaactacc tgaacaaaaa tgttattcct tatgaagcaa tactgaaaac cagggagccg
     3301 caggcggtca ttggaaatgc ctaccacccc ttcaaccggt cgaactactc gctttacggc
     3361 cttggatggg aactacagga ttatgaaggc catggtctgg tgatgcacaa tggaggcatc
     3421 gacggttttg tcacctcagt aacgctggtg cccgatgaaa accttggtat cgttgtgcta
     3481 accaacaccg atcagaatta tttatatgaa gccctgaaat gggagatcct cgatgcttat
     3541 ctgaacctac cttacagaaa ctatagcaaa ctttacaaca attatttcaa acgcagggca
     3601 caacgcgata ataatgaaat caaagtatgg cgcgattcgg caaccatgaa tatcaccctg
     3661 cccctgcccc tttcggaatt taccggcatc taccaaaatg aagtttatgg aaccatcacc
     3721 ataaaaaaag aagccggtct gctgaaacta aactttgaac atcacaaaaa tctctcggca
     3781 aaattagaat acataggcaa caaccgattt ttatgcactt acagtcagcc attgtttggt
     3841 attaaagtat tcccgtttgt cgttgagggg ggaaaagtaa catctttcac tctgagtgtg
     3901 gcagattttc tggaatatac tacctacaag ttcacaaaac agaaatgacc atccccgtga
     3961 aatcaggcac tgctcaacac ctcgggtata aagcaacaat ttttagttca gtatcaataa
     4021 actatgatcc ttgtatatca tgacctggct gaataacctg ttttttaacg attcggtggc
     4081 acataccatt ttggtattca gcatggtgat agccataggc gttttgctgg gcaaaataaa
     4141 aatcttc
//
LOCUS       DAOSFP010000205         4126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19618_length_4126_cov_4.077868, whole genome shotgun sequence.
ACCESSION   DAOSFP010000205 DAOSFP010000000
VERSION     DAOSFP010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19618_length_4126_cov_4.077868"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLI16_06825"
     CDS             complement(<1..1065)
                     /locus_tag="PLI16_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH84311.1"
                     /translation="MNTELFIARRIISRNKANFSRPIVRIGILSIALGVAVMIVAVAI
                     VTGFQKEIRNKVIGFGGHIVISSYDENVSMESSPVSTNQAFYPSLEQEDGVRHIQVFG
                     TKAGIIKTDEDIQGVVLKGVGGDFDWSFFSDKIIRGSAIQVSDTGKTNDILVSENLSL
                     KLKLDTGQSLLMYFINQEQAQPSVRKFRISGIYNTGLEEFDKVYVLGDIKHIQRLNNW
                     DSTQVGGFEVLIDDFSELDKITESVYNRIGYDLNCKSIKEMYPQIFDWLDFQDVNVTI
                     IIVMMVIVAAINMISALLILIIEKTTLIGILKALGMRNWSIRKIFLYNATYLIFRGLV
                     WGNIIALALCFVQKFFGVIKL"
     gene            1184..2404
                     /locus_tag="PLI16_06830"
     CDS             1184..2404
                     /locus_tag="PLI16_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOH84312.1"
                     /translation="MRIFNWFSFLFLLSVCTSSCTGQNFINEINGVDIYPGDIRVGAE
                     RTEVYFPWLKDKRIAVVANQTSMIKNVHLVDSLVHAGFDVKKVLCPEHGFRGDAEAGE
                     TVGNTVDKKTGLPVVSLYDSNKKPKAKDLDDVDVVVFDIQDVGVRFYTYISTMHYVME
                     ACAENKKLFLVLDRPNPNGYYVDGPVLEKDFSSFVGMHPVPIVHGMTLAEYALMINGE
                     KWLKDSVRCAMKYVLIDTYRHSDFYQLPVKPSPNLPSMESIYLYPSFCLFEGTCISVG
                     RGTERPFEIIGNPLLDSTGFSFTPRSIPGMSKNPPHEGKICHGYDFHSYADNFIKGYG
                     KLYLFPLLETYKMLKDKTEYFNSFFITLTGTAKMKQQIINNASEEQIRQSWQNEIEKF
                     KKIRKKYLLYPDFE"
     gene            2416..>4126
                     /locus_tag="PLI16_06835"
     CDS             2416..>4126
                     /locus_tag="PLI16_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH84313.1"
                     /translation="MPKAKTKQLHKNKEKTTKGTARQSGGKKNYFSATQLQYLVILLI
                     SLLIYANTLFFGFALDDGLIITDNKVTKKGLAGYKEIFTKNAFYGVFGDQASIYLPGG
                     RFRPMSQAMFAAEYQIFGLSPFAGHFFNIIIYGMLCLLLLKILKSVFEKYDNNPWYLS
                     LPFVATILFACHPLHTEVVANIKGRDELLSLLGALATLYFAINYIKNQKFHNLLFIFL
                     AFAFALLSKENAVTFFAVVPLAIFFGYNEPLKKYIRVLIPMLAAFGLYFLLRYQALGF
                     IQNPVESKELLNNPFLYAEGTEKFATILFTWGKYLMLVLWPHPLTHDYYPYQIPIIDF
                     PDIRALAPLIIYLALMIFSMVKIFSKNVFAFAILFFLITFSIASNLVFNIGTFMNERF
                     MFAPLLGFTIVVAFGLNRHLTKPKRLTIATIFLVVLVSAYSVKTFSRNFAWKDSYTLF
                     TTDVNNSPNSAKVNAGAGEMLLRNVNDATAEPLRTQTIEKAIGYLKKSVDIYPGFKSG
                     WVFLGYAYHLQKDYENSRTVLEQALKLENNNSDAIGYLNSAALECYRTKNYKQSEANF
                     KTLI"
BASE COUNT         1190 a          939 c          813 g         1184 t
ORIGIN      
        1 aagtttgatg acaccgaaaa atttctgtac aaaacaaagc gccaaagcga tgatgttacc
       61 ccagacaagt ccgcggaaaa tcaaataagt ggcgttgtac agaaatatct tccggatgct
      121 ccagtttcgc atgcccaggg cttttaaaat gcctatcagg gttgttttct cgatgatcag
      181 aatgagcagg gcagaaatca tattgatagc agccacaatc accatcatta caatgatgat
      241 ggtaacattc acatcctgaa aatcaagcca gtcgaatatc tgtggataca tctccttaat
      301 acttttacag ttaaggtcgt agccaatcct gttatacacc gattcggtga tcttgtccag
      361 ttcgctgaaa tcgtcaataa gaacttcaaa ccctcctacc tgcgtagagt cccagttgtt
      421 gagccgctga atatgcttaa tatctcccag cacataaacc ttgtcaaatt cttccagtcc
      481 ggtgttgtat attcccgaaa tacgaaactt gcgcacacta ggctgagctt gctcctggtt
      541 gataaaatac atcagcagcg actgtcctgt atctaatttc aacttcagcg acaaattctc
      601 cgacaccagt atatcgttgg ttttaccagt gtcactcacc tgaattgccg accccctgat
      661 aattttgtcg ctgaaaaaag accagtcaaa atcccctcct actcctttca acactacgcc
      721 ctgaatatct tcgtcagttt ttattatgcc tgccttggtg ccaaaaacct gaatatggcg
      781 tactccgtct tcctgttcaa gagatgggta aaaagcctgg tttgtgctga ccggcgaaga
      841 ttccatcgac acattctcat catagcttga aatcacgata tggccgccaa aaccaattac
      901 cttgtttcgg atctctttct gaaacccggt aacaatggca acagccacta tcatcacagc
      961 cacccccaaa gcaatgctca gtatgcctat gcgaacaatg ggccgggaaa aatttgcctt
     1021 gttcctggaa atgatgcgac gggcaataaa taattcggtg ttcaaactga aaattttatt
     1081 attttgcaaa agtatttaaa actgcaataa gttctataat tattattgcc ttattcattt
     1141 actgctaagg cttgaagaca aaaaacaaac agggtacccg gatatgagga tttttaattg
     1201 gttctctttt ttatttcttt taagtgtttg cacttcttcg tgtaccggac agaattttat
     1261 caacgaaatt aatggggttg atatttatcc tggtgatatc agggtgggcg ccgaacgaac
     1321 agaagtatat tttccctggc tcaaagacaa aagaattgct gttgtagcca atcagacttc
     1381 catgataaaa aatgtccacc ttgttgattc gctggttcat gcaggctttg atgtaaaaaa
     1441 agttttgtgc cccgagcacg gtttccgtgg tgatgccgaa gccggcgaaa ccgtaggaaa
     1501 tactgtggat aaaaaaacgg ggctgcccgt ggtttcatta tatgacagca ataaaaaacc
     1561 caaagccaaa gacctcgacg atgtggatgt tgtggttttc gacatacagg atgttggtgt
     1621 ccgtttttat acctacattt ccaccatgca ctatgtgatg gaagcctgtg cagaaaacaa
     1681 aaaactcttc ctggtgctcg accggcccaa tcccaatggc tattatgttg acgggcctgt
     1741 gctggagaaa gatttttcat cctttgtagg tatgcaccct gttcctattg tccacgggat
     1801 gacgcttgcc gaatatgccc ttatgattaa cggcgaaaaa tggctgaaag acagtgtgcg
     1861 atgcgctatg aaatatgtgc ttattgacac ctaccggcac tctgattttt accagctccc
     1921 ggtgaaacct tcgcccaacc tgcccagcat ggaatccatt tatctgtacc cttcgttttg
     1981 ccttttcgag ggcacgtgca tcagcgtagg gaggggaacc gaacgccctt tcgagataat
     2041 tggtaacccg ctgctcgaca gtacaggttt ttctttcaca ccgcgaagca tcccgggtat
     2101 gtcgaaaaat ccgccgcacg agggaaaaat ttgtcacggt tatgattttc acagctatgc
     2161 tgacaatttc atcaaaggtt atggcaaact gtatttgttc cctctgctgg aaacatacaa
     2221 aatgctgaaa gacaaaaccg agtattttaa tagtttcttc atcaccctga cgggaaccgc
     2281 caaaatgaaa cagcagatta ttaataatgc ctccgaagag caaatccggc aaagctggca
     2341 aaatgaaatt gaaaagttta aaaaaatccg aaaaaaatat ttgctgtacc ccgattttga
     2401 ataatccttt caaatatgcc gaaagcaaaa acaaagcaac tacacaaaaa caaggaaaaa
     2461 acaactaaag gaacagccag gcagtccggc ggaaaaaaaa actatttctc cgccacacag
     2521 ttgcaatacc tggtgatact tctcatcagt ttattgatat atgcgaacac gctatttttc
     2581 ggattcgcat tggacgacgg actgataatc accgacaaca aggtgaccaa aaaaggattg
     2641 gccggttaca aagaaatttt tacaaaaaat gcattctacg gcgttttcgg cgaccaggcc
     2701 agcatatatc ttccgggagg aaggttccgt cccatgtcgc aggccatgtt tgctgctgaa
     2761 tatcagattt ttgggttaag tccttttgca ggacattttt ttaatattat catctacggc
     2821 atgctgtgcc tgctcctcct caaaattctt aaaagtgttt ttgaaaaata cgacaacaac
     2881 ccctggtacc tgtcgcttcc ttttgtcgcg accatcctgt ttgcctgtca tccattgcat
     2941 accgaagtgg tggccaacat caaagggcgc gacgaactgc tttctctgct tggagcatta
     3001 gcaacgctat atttcgccat taattacatc aagaaccaga agtttcataa cctgctgttt
     3061 atttttctgg ctttcgcttt tgccttactc tccaaagaaa atgcggtaac tttttttgct
     3121 gtagtgcccc ttgctatatt ttttggatac aacgaaccat taaaaaaata catccgggtg
     3181 cttatcccta tgctggccgc ctttggtttg tattttctgt tgcggtatca ggctttggga
     3241 ttcatacaaa acccggtgga atcaaaggaa ctgctcaata atcccttttt atatgctgag
     3301 ggtacagaaa aatttgccac catcctgttt acctggggca aatacctgat gctggtttta
     3361 tggccccacc cgcttaccca tgattactat ccctatcaga tccccattat agattttcct
     3421 gatatccgtg ccctggcgcc actcataatt tatcttgcac tgatgatttt cagcatggta
     3481 aaaatctttt caaaaaatgt gttcgctttt gcaatattgt tttttctgat tactttttcc
     3541 attgcttcca atttagtttt taatatcgga acattcatga atgaacgttt catgttcgct
     3601 cccctgctcg gtttcactat cgttgtagct tttggtctca ataggcacct gacaaaaccg
     3661 aagagactga caatagcaac aatttttctt gttgtgctcg tgtcggcgta ttcggtaaaa
     3721 accttcagcc ggaattttgc atggaaagac tcctatactt tgttcactac tgatgtgaac
     3781 aattcgccca acagcgctaa agtaaacgcc ggagccggag aaatgctcct cagaaacgtg
     3841 aacgatgcta cagcggaacc cttacgtaca caaactattg aaaaagcaat cggatatttg
     3901 aaaaaaagcg tggatatcta tcccgggttt aagtcgggat gggtttttct tggttatgca
     3961 tatcatttac aaaaagatta tgaaaacagc cgtaccgtcc ttgaacaggc attgaaactc
     4021 gaaaataaca attccgatgc tataggatac ctgaattccg ccgcgttgga atgttaccgg
     4081 acaaaaaact ataaacagtc ggaggccaat tttaaaacac tgatca
//
LOCUS       DAOSFP010000206         4120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19654_length_4120_cov_3.686593, whole genome shotgun sequence.
ACCESSION   DAOSFP010000206 DAOSFP010000000
VERSION     DAOSFP010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19654_length_4120_cov_3.686593"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="PLI16_06840"
     CDS             complement(<1..838)
                     /locus_tag="PLI16_06840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH84314.1"
                     /translation="MKFDEFGFDPRLLEGIEAIGYQAATPIQEKAIPFIMQGRDIIGS
                     AQTGTGKTAAFLLPIIHKIITSEHHDHIKAMIIVPTRELATQIDQQMEGFSYFTPVSS
                     IAVYGGTDGISFGNEKQSLTEGVDVVICTPGRMIAHLNQGYTDLSHLTHLVLDEADRM
                     LDMGFYDDIMQIISYMPKNRQNLMFSATMPGQIRQLAQKILHEPEEVSIALAKPSEKV
                     LQLAYVIYRSQKVPLVLHLLASEKLGSVLVFCSTKMSAKQLCRELKQKGIKAEDIHSD
                     LNQ"
     gene            complement(878..1303)
                     /locus_tag="PLI16_06845"
     CDS             complement(878..1303)
                     /locus_tag="PLI16_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOH84315.1"
                     /translation="MLTTKEDIIDFIRKNPLAFVATTDGSQPRVRGIMIHDVDEKGIV
                     LTTGKNKDFYKQLTTNPRVELCFYGNNTQVRVTGMAVEADNELELKKEIVSSRPFMQP
                     WIEMAGYGIMGLFRISQCKATAWSSDSPMARKRFVELFA"
     gene            complement(1297..2235)
                     /locus_tag="PLI16_06850"
     CDS             complement(1297..2235)
                     /locus_tag="PLI16_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84316.1"
                     /translation="MTASLSNEKSGHKKTAHLFPAFVPEYLGIETSVIESYGIKLQPY
                     LDSASELTGENLSEFDIIHNNLLGNQLKSQYITYIISCCISDLLHEKKILPDYISSYS
                     MGIYAALYHCGSIDFGTGLQMITAAFSAIENNLPAHPQAMCSIGGLNSKDIREIMQPY
                     IEDVYIINQNSEYSFLLSGQKNSLEHIVQGAVKMGALQTRMLPVTHPYHSPLLKNAST
                     VFNNVIKKMSIGENRLPYVSAVTREIIKGRNDIIAELTSNIFNGFHWHKTFLFLLDKG
                     VDTFIECGAGDSLYRLGKFIEGDFNIVTLKKIHKIC"
     gene            complement(2392..3147)
                     /locus_tag="PLI16_06855"
     CDS             complement(2392..3147)
                     /locus_tag="PLI16_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HOH84317.1"
                     /translation="MHRTHTIAAVSYANTFPFVYGIENSQMIDNFQLQLFPPAGCAAS
                     FAAGDADIALVPVGALPTLSGYRIISEYCIGAVKEVKTVLLLSNKKIKDIRSIGLDHE
                     SATSVKLVKIFARHHWHIQPAWQVMEVCNPEAYQQTDAVVAIGDKAISLSKKFLYQTD
                     LAAEWRNFTGLPFVFAVWICRNDLPAAMENSFSAALQYGIDNISKVVEKYKTRLAADF
                     DLEGYYQHNIDYHLDKEKKQSLQKFLTFVAEIV"
     gene            complement(3206..>4120)
                     /locus_tag="PLI16_06860"
     CDS             complement(3206..>4120)
                     /locus_tag="PLI16_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84318.1"
                     /translation="ADTVREGNAGQNQVWDFSMLTATGEMTSYMFASGNHPESALYPE
                     ANMVLREKNMDYFFKVGPSGMAEYGHSTEAFKVIYDAPIVKFPFPFTYGSAINGIFSG
                     KQIINNQTTAVTGTYSTLADGYGTLILPGNIRIPNVLRVKFVRQSNNSQSAAIAYRWY
                     AQNADPVVRYPLLTIIVIQTGSKVRVQTVAYYAYAYQYAQNQANNYKPEVPGEYLSDV
                     AEKFSIKISPNPFVDKAQIEYSLPEESTVSIQIFDNTGRLLEQLAEGKKPAGPHSVAF
                     TGKGQFVYFVRIVINDNIICSKKIIQMK"
BASE COUNT         1027 a          896 c          956 g         1241 t
ORIGIN      
        1 tctggttaag atcggaatgg atatcttctg ctttaatacc tttttgtttc aactcacggc
       61 atagttgttt tgcactcatt tttgtggaac aaaacacaag caccgatccc aatttttcag
      121 aggccagcag atgcagtacc aaaggcactt tctgtgaacg gtaaattacg taggccagtt
      181 gcagcacctt ttccgacggt ttggccagag ctatgctgac ttcttcgggc tcatggagta
      241 ttttttgtgc caactgccgg atttgtccgg gcatggtagc cgaaaacatc aggttttgtc
      301 tgtttttagg catgtaagag atgatctgca tgatgtcatc ataaaaaccc atatcgagca
      361 tgcggtcggc ttcgtcaagt accagatggg tcaggtgcga caggtccgta tagccctggt
      421 tcaggtgggc tatcatacgt ccgggagtgc aaatcaccac atcaacacct tcggtaagcg
      481 actgtttttc gttgccgaac gaaatgccgt cggtaccgcc atacaccgca atggaactca
      541 caggtgtaaa gtatgaaaag ccctccatct gctgatctat ctgggtggcc agctcgcggg
      601 taggtacgat tatcattgct ttgatatggt cgtggtgttc gctggtaatg attttatgga
      661 tgataggcag caaaaatgct gcggtttttc ctgttccggt ctgggccgac cctataatat
      721 cacgcccctg cataataaac ggaatagctt tttcctgtat cggtgtggcg gcctgatagc
      781 caatagcttc tatgccttcg agcaggcggg ggtcaaaacc aaattcatca aatttcaaag
      841 cagtgtattt ttttgtaaag gtacgatatt ccggcaatca ggcgaataat tccacgaagc
      901 gttttctggc catcggagaa tcagagctcc acgcagtagc tttgcactga cttatcctga
      961 aaagccccat aatcccgtat cctgccattt ctatccaagg ctgcataaac ggtcgtgaag
     1021 acacgatctc cttttttagt tccagttcat tgtcggcctc tacagccatt ccggtaacgc
     1081 gaacctgtgt gttgttccca taaaaacata attccacgcg gggattggtt gtcagctgtt
     1141 tgtagaaatc tttatttttt ccggtggtta agacaatacc cttttcgtcc acatcatgta
     1201 tcataatgcc gcgcacacgg ggttgacttc catcggttgt tgctacaaag gccagggggt
     1261 ttttccggat aaaatctata atgtcttctt ttgtcgtcag catattttat gaattttctt
     1321 taacgtgaca atattgaaat cgccttcaat aaacttcccc aatctgtaaa ggctgtcgcc
     1381 ggctccgcat tcaatgaacg tatccacgcc tttgtcaagc aaaaacaaaa atgtcttgtg
     1441 ccaatggaaa ccattaaaaa tattacttgt aagttctgca atgatgtcgt tgcggccttt
     1501 aataatttcc ctggtgacag ccgaaacata gggcagccgg ttctcaccga tgctcatttt
     1561 ttttatgaca ttattaaaaa cagtggatgc gtttttcagc aaaggcgagt ggtaaggatg
     1621 tgtaacaggc agcatgcggg tttgcagggc ccccattttt acagcaccct gcactatgtg
     1681 ttcgagactg tttttctggc ctgacagcag aaatgaatac tcgctgtttt ggttaatgat
     1741 gtaaacatcc tcaatgtaag gctgcatgat ttcccggatg tctttgctgt tcaagcctcc
     1801 aatggaacac atggcttggg gatgtgcagg caagttgttt tcgatggcgc tgaaagctgc
     1861 tgttatcatc tgcagtcccg tgccaaaatc aatggagccg caatgataca aagccgcata
     1921 aatacccata ctataagaac tgatataatc cggcagtatt tttttttcgt gtaaaaggtc
     1981 cgaaatgcag cagctgataa tgtaggtgat gtattgtgat tttagttgat tgccgaggag
     2041 gttgttgtgt atgatgtcaa actcactgag gttttctccg gtaagttccg aagcgctgtc
     2101 gagataaggc tgcagcttga ttccgtaact ttcgatgacg gaagtttcta ttcccagata
     2161 ttcgggaaca aaagcaggaa acagatgagc tgtttttttg tggccggatt tttcgttgct
     2221 tagagaggct gtcatagatc ttttttgtct cggagtttat tcggattcat tcctgatatc
     2281 aaaagattag ttcaaggttt taccagaata caaagcgtat atggcgattt gtagaacgaa
     2341 gacctacacg gaattttaaa gttttagccc gcttcataaa caatgaatca tttaaactat
     2401 ttcagcaaca aaagtaagaa atttctgcaa ggactgtttt ttctctttgt ccaaatggta
     2461 gtctatgttg tgctgataat aaccctccag atcgaaatct gctgcaagtc gtgttttgta
     2521 tttttccaca accttactga tgttgtcaat gccgtattgc agggcagccg aaaaactatt
     2581 ttccattgcg gcgggaaggt cattacggca tatccatacg gcaaatacaa agggcagtcc
     2641 ggtgaaattt ctccattcgg cagcaaggtc ggtttggtac aaaaattttt tcgacaggct
     2701 tatcgcttta tctccaatgg caaccacggc atcggtttgc tggtatgctt ccgggttgca
     2761 gacttccatt acctgccagg ccggttgtat gtgccagtgg tgccgggcaa atattttgac
     2821 aagctttacc gaggtggctg actcatgatc caggccaata gaccggatat cctttatctt
     2881 tttattgcta agcaagagta cggtcttcac ttcttttacc gcgccaatac aatattccga
     2941 aatgattctg tatccgctta gtgtgggtag cgcaccaaca ggcaccaggg caatatctgc
     3001 atctccggcg gcaaaactgg ctgcacaccc ggccggcgga aacaactgca attgaaaatt
     3061 gtcaatcatt tgtgagtttt ctataccata aacaaacgga aaagtgttgg cgtatgaaac
     3121 ggctgctatg gtatgcgtgc ggtgcatgtt gttctgggaa ctattgattt tgctctcttg
     3181 ttttattggc tacttttggc aagtgttatt tcatctgaat gattttcttc gaacagatga
     3241 tgttgtcatt aatcacgatg cggacaaaat acacaaactg gcctttacct gtaaaagcta
     3301 cgctgtgcgg gcctgccggt tttttccctt cggcgagttg ttccagcagg cggcctgtgt
     3361 tgtcaaaaat ctggatggaa accgtggatt cttcgggaag ggaatattct atctgtgctt
     3421 tatcaacaaa aggattgggc gatattttta tgctgaattt ttcggcaaca tccgacaggt
     3481 attcgcccgg aacttccggc ttgtagttgt tggcttggtt ttgagcatac tggtaagcgt
     3541 aggcataata agccactgtt tgcactctca cttttgagcc tgtttgtatt acgataatgg
     3601 tcagcagcgg atatctgacc acaggatcgg cgttttgtgc ataccagcgg taggctatgg
     3661 ccgccgactg gctgttatta ctttgcctga caaattttac cctcaaaaca ttgggtattc
     3721 ttatgttccc gggcagtatc agtgtgccat atccgtcggc cagagtggaa taagtccctg
     3781 taacggcagt ggtttgattg ttgatgattt gtttaccgga gaaaatgccg ttgatagccg
     3841 agccatacgt aaaaggaaaa ggaaatttca cgataggtgc gtcgtaaatc accttgaacg
     3901 cttcggtgga atggccatat tctgccatac ctgatggccc gactttgaaa aaatagtcca
     3961 tatttttttc gcgcagtacc atattggctt cgggatacaa cgcactttca gggtggttgc
     4021 ccgaagcaaa catataactg gtcatttcac ctgtagccgt cagcatgctg aagtcccata
     4081 cctgattctg cccggcattg ccttccctta ctgtgtcggc
//
LOCUS       DAOSFP010000207         4116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19676_length_4116_cov_3.826151, whole genome shotgun sequence.
ACCESSION   DAOSFP010000207 DAOSFP010000000
VERSION     DAOSFP010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19676_length_4116_cov_3.826151"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLI16_06865"
     CDS             <1..324
                     /locus_tag="PLI16_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84319.1"
                     /translation="QDCLNGMFFFQKNKDGQVEWLQPMTSAGNSHEIYGNWSLVEISL
                     EPADAEASYELVYKGSDLAEKTVYMDDLLFYDDKLAIYKMLVDQGTSCLFKNNHRINS
                     PEKTR"
     gene            complement(411..2468)
                     /locus_tag="PLI16_06870"
     CDS             complement(411..2468)
                     /locus_tag="PLI16_06870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOH84320.1"
                     /translation="MKTKFNKYLLIVLSSIIGAGMMNACKNKNMKQEEPAINIANMDT
                     TVKPGDDFFRYANGQWMKNNPIPDEYSIYGSFEELRETNNKQLRTIMEEAMADKNATK
                     GSVKQKIADFYSSGMDSAAIEKMGIKAIQKELEKIDAIKNPADLFNLVAFFHSSGITP
                     LFYFFSGQDDKNSEQVIAQLYQGGLGLPDRDYYLAQDARSSEIRAEYVKHLGKTFELA
                     GTNKEQSTLDAATVMQIETRLAKASLTRLELRDPIKNYNKTDLAGLKKMAPQFDWATY
                     FTDLGLKATGEINVGQPQFFAEMAKMSTEISIEQWKTYFKWHLLHEASPYLSAAFEKE
                     QFNFYGGILSGIKEMKPRWKRVLNTTSSSLGEAVGQLYVEKYFPVKAKERMLTLVGNL
                     KEALKERIEKLSWMGEATKKEAVAKLAKMNLKVGYPDKWRDYASLDITKESYFQNIMA
                     ASRFEFARDLNKIGKPVDRSEWGMTPQTVNAYYSPNMNEIVFPAAILQPPFFFMNGDD
                     AVNYGGIGVVIGHEMTHGFDDQGRQYDKDGNLRDWWTEEDATNFKKQTQVLVDQFNEF
                     KMLDSLHVDGQLTLGENIADMGGITVALVALKKAMKDSDMNAKLEGFTHLQRFFLSYA
                     QIWRGNTRDKELMRRLKEDVHSPKEARVNGQVYNIPEFYTAFDIKPTDKFYKAENLRP
                     VIW"
     gene            complement(2583..3269)
                     /locus_tag="PLI16_06875"
     CDS             complement(2583..3269)
                     /locus_tag="PLI16_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84321.1"
                     /translation="MKINLFPTKILISVLVFFIIFISSCTIDETPTKTRMEGVWTVKH
                     VYDQSGEDHASQLNFPITAFHLSSDNTVISTAGPLTMFVVYGNSKYVQIAADIDQVFN
                     YAGLDFNGGEFFVEGGLVDRFTLEMKLEGLPGQGALTDLLDMLGVTNDYLDMVIYHKF
                     INVGVYFNEDNTIMTWVFDDQTTAVYNTKDNQGNYLLWNGWPVQNFTRCTIELQKQTK
                     DLQQVVTDAI"
     gene            complement(3288..>4116)
                     /locus_tag="PLI16_06880"
     CDS             complement(3288..>4116)
                     /locus_tag="PLI16_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84322.1"
                     /translation="GSFNYSNTEMTYGILGTINDNANGKISTLAYCPEGMGHHGRNIL
                     KALKNGHTILSDGPIINLGISTDGADSTNEIFIGQDTVLTPQQLINSRLVVDSYITPE
                     FGNLTQITLTGITEDSIFTLELPLIAHQVLDLQSVLGHLFGNIPDNHYFMIRASLRTT
                     KNYGILSTIYRRPYDRFFSITNPIWIKTPMLTSADDNTIPEEIITRPNPVYDRFLVNL
                     PGNKNYYVKIFDMNGRLLLEEPYSNAGVDVRKLPSGLYLATFINDKNIFRKKIIVSH"
BASE COUNT         1018 a          869 c          962 g         1267 t
ORIGIN      
        1 caggattgtc ttaatggaat gtttttcttt cagaaaaaca aggacgggca ggtggagtgg
       61 ctgcagccga tgaccagtgc gggcaacagt catgaaattt acggcaattg gtcgctggtg
      121 gaaatatcat tggagcctgc cgatgcggag gcctcatacg agttggtata taaaggcagc
      181 gatctggctg agaaaaccgt gtatatggat gacctgctgt tctatgatga caaactggcg
      241 atatataaaa tgcttgttga tcaggggact tcttgcctgt ttaagaataa tcaccgcata
      301 aatagtcctg aaaaaacaag ataaggctca ttttttgccg caattcctgt ttttagcacc
      361 aaccccaatc gggagatgtc accaaggcag ttgctgaaaa attatcagtg ttaccagatt
      421 accgggcgca ggttttccgc tttgtaaaat ttgtctgttg gcttgatatc aaaagcggta
      481 taaaattcag gaatattata aacctgaccg tttacacggg cttctttggg cgaatgtaca
      541 tcttctttca gtcgtcgcat taactcttta tcgcgggtat tccccctcca aatctgggca
      601 tacgaaagaa aaaaccgttg caggtgggtg aagccttcaa gtttggcatt catatcgctg
      661 tctttcatgg cttttttcag ggctacgagg gccacggtaa taccgcccat atcggcaatg
      721 ttttcgccca gagtcagttg gccatccacg tgcagcgagt ctaacatttt aaattcgttg
      781 aactggtcca ccagaacctg tgtctgtttt ttgaaattcg tggcgtcttc ttctgtccac
      841 cagtcgcgta ggttgccgtc tttatcgtat tgccgtccct gatcatcaaa gccgtgtgtc
      901 atctcgtggc caatcaccac gccaatgccg ccgtagttaa cggcgtcgtc gccattcata
      961 aagaaaaacg ggggctgcag gatggctgcc ggaaacacta tttcgttcat gttggggctg
     1021 tagtaggcgt tgacagtctg tggagtcata ccccattcgc tgcggtcaac cggtttgcct
     1081 attttgttca gatcacgcgc aaattcaaag cgtgaagccg ccatgatatt ctgaaaataa
     1141 gactctttgg ttatgtcaag tgaggcatag tccctccatt tgtcaggata accgaccttt
     1201 aaattcatct tggccagttt ggccacggct tctttttttg tagcttctcc catccacgaa
     1261 agtttttcaa tcctttcctt cagggcttcc ttaagatttc ccaccagggt gagcatacgt
     1321 tctttggcct ttaccggaaa atacttttcg acataaagtt gaccaacggc ttctcctaaa
     1381 gaactgcttg tggtatttaa cacccgtttc cagcgcggtt tcatttcttt tatacccgaa
     1441 aggattccgc cgtaaaagtt aaactgctct ttttcaaaag cggccgaaag gtatggtgaa
     1501 gcttcgtgca acagatgcca tttgaaatat gttttccatt gttctatgga gatttcggtg
     1561 gacatctttg ccatttctgc aaaaaactgt ggctggccca cattgatttc gccggtagcc
     1621 tttagtccga gatccgtaaa gtaggttgcc cagtcaaatt gtggcgccat ttttttcagg
     1681 ccggccaggt ctgttttgtt gtagtttttt atcgggtcgc gcagttccag gcgtgtgagc
     1741 gaggctttag ccaggcgggt ttcaatttgc atcaccgttg ccgcgtcgag ggttgattgt
     1801 tctttatttg tgccggctaa ttcaaaagtt ttgccgaggt gtttcacata ttcggcgcgt
     1861 atttcggacg aacgggcatc ctgagccagg tagtaatccc tgtcgggaag gccaaggcct
     1921 ccctggtaga gctgagcaat aacctgttcg ctgtttttgt cgtcctgccc tgaaaaaaaa
     1981 taaaacaagg gggtaatgcc cgacgagtgg aaaaaggcta ccaggttgaa caggtctgcc
     2041 ggatttttta tggcatcaat tttttcaagt tctttctgta tggcctttat acccattttt
     2101 tctatggcag ccgagtccat acccgaagaa taaaaatcgg caatcttttg ttttacagaa
     2161 cctttagtcg catttttatc tgccatggcc tcttccatga tagtcctgag ctgtttgttg
     2221 ttggtctccc ttaattcttc aaaagagcca taaatgctgt attcatccgg aatggggttg
     2281 tttttcatcc attggccgtt ggcatacctg aaaaaatcat caccgggttt cacggtggta
     2341 tccatgttgg caatgttaat tgccggttct tcctgtttca tgtttttgtt tttacacgcg
     2401 ttcatcatac ccgcgcctat gattgaggag agcacgatga gtaaatactt attgaatttg
     2461 gttttcataa aaggttgttg tttttggttt ggaattattg cggcacaaaa taaatcattt
     2521 aattaaaaca aaaaacagca aaagataagt cttctgctgt ttttttactg acgaaatttt
     2581 ttttaaatgg catcggttac tacctgttgc aggtctttgg tttgtttttg caattcgatg
     2641 gtgcaacggg taaaattttg aaccggccag ccattccata aaaggtaatt tccctggttg
     2701 tctttagtgt tgtaaacagc agtggtctga tcgtcaaaaa cccaggtcat gatggtgttg
     2761 tcttcattga aatatacccc gacattgata aatttatgat aaatcaccat gtcgaggtag
     2821 tcgtttgtca cccctaacat atccagcaag tcggtaagag caccctggcc gggcaggcct
     2881 tccagtttca tttccagggt aaaacggtcc accaggcctc cctcaacaaa aaattcgccg
     2941 ccgttaaaat ccagcccggc ataattaaac acctggtcga tgtcggcggc aatctgcacg
     3001 tatttgctgt tgccatacac tacaaacatg gttaaaggcc cggctgtgga aatcaccgtg
     3061 ttgtcgctgc tcaggtgaaa ggcggtaata ggaaagttca actgcgaggc atggtcttcg
     3121 cccgattggt cgtacacatg tttcactgtc cacactccct ccatccttgt ttttgtaggg
     3181 gtttcgtcaa tagtacagga agaaatgaaa ataatgaaaa ataccaatac gctgataagg
     3241 attttggttg ggaataaatt tattttcatg attttttgat ttaatttcta atggctgaca
     3301 attatttttt tgcggaaaat gtttttatca ttgataaagg tagccagata taagcctgaa
     3361 ggtaacttcc gcacatcaac accggcattg gaatagggct cttcaagcag caggcggcca
     3421 ttcatgtcaa aaatcttcac ataataattc ttattacccg gcaaatttac taaaaatctg
     3481 tcatatacag gattaggcct tgtgataatt tcttcaggta tggtgttatc atcagccgag
     3541 gttagcatgg gggtttttat ccatattggg ttggtgatac tgaaaaaacg gtcataaggc
     3601 cggcggtata tggtgctgag tataccataa ttttttgtgg tacgcaggga cgcccgaatc
     3661 ataaaatagt ggttgtcggg gatattgcca aataaatgcc ccaaaacgct ttgcaggtca
     3721 agtacctgat gggcaattaa ggggagttct aatgtaaaaa tgctgtcttc agtgattcct
     3781 gtgagggtga tttgtgttaa atttccgaat tcgggcgtga tgtaggaatc cactaccagc
     3841 cgtgagttga ttagttgctg tggtgtcagc acggtgtcct ggccgataaa tatctcgttg
     3901 gtgctgtcgg cgccgtcggt gctgatccca aggtttatta tggggccgtc agaaagtatg
     3961 gtgtggccgt ttttcagtgc ttttaggata tttctgccgt gatggcccat accctcgggg
     4021 caatatgcca gcgtggaaat tttgccgtta gcattgtcgt tgatggttcc aagtatgcca
     4081 tatgtcattt cggtattgga gtaattaaag gaacca
//
LOCUS       DAOSFP010000208         4102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19768_length_4102_cov_3.993823, whole genome shotgun sequence.
ACCESSION   DAOSFP010000208 DAOSFP010000000
VERSION     DAOSFP010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19768_length_4102_cov_3.993823"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLI16_06885"
     CDS             complement(<1..717)
                     /locus_tag="PLI16_06885"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOH84323.1"
                     /translation="MFRNIFFKFWLGVFLAGGFLISLRLPAQLTVVQGTALGMTPIQL
                     VQNVLVGTGVTVSNGLFNGSAANISSNMIGSFNAAGVAATQLGFAQGIIITSGQGSNA
                     IGPNNTGSSGTGSATGSDPDLQALVPSNTVYDKAVLEFDFIPIADTIKFQYVFGSEEF
                     DEFCNSSYNDVFGFFVSGPGISGPFSNSSVNIAKMPNNPANYVTINNVCNAGATYSWS
                     NASGTYFQYDRLTKVYTAWIL"
     gene            1057..3321
                     /gene="pbpC"
                     /locus_tag="PLI16_06890"
     CDS             1057..3321
                     /gene="pbpC"
                     /locus_tag="PLI16_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOH84324.1"
                     /translation="MMDDCQGVLLGARIAADHQWRFPAGKTVPEKFQKAVIMFEDRFF
                     YYHNGLNPWAMARAAYQDIKAGKIVSGGSTITMQVIRLSRKGRPRTVFEKIAEAFLAV
                     RLELTYSKEEILALYAANAPFGGNVVGIDAASWRYFGKNAAQLSWAEAATLAVLPNSP
                     ALIYPGKSHDKLLKKRNKILNKLYKNNYISQEEFLLAQNEPLPGKPYPLPQAASHLLD
                     RSIKEGYSGKRIKTTLDIGLQTRVLSIIERYHEKYSANKVNNAAVLVVEVETGNVLAY
                     VGNTTETNHDGNGCQVDIITSPRSTGSILKPFLYAAMINDGQLLPAALVPDIPVQLGG
                     FIPENYSLTYDGAVPAKRALSRSLNVPAVKMLQAYGINRFNYVLKKIGLTTLHKPPSH
                     YGLAIILGGAEANLWDMTGAYASMARTLNHYQPYDSRYDKGDFRPPVYIFQDAKKKSP
                     GTEKSSVLSASSIWFTFEAMNEVSRPDEDMEWQQFSSSAKIAWKTGTSYGSRDAWAIG
                     VNPRYVVAVWTGNSDGEGRPGMTGLTYAAPIMFEVFKSLKPEGWFYAPYDDMIRLPVC
                     RHSGFKATAVCEEVDTVWVQKNGAKSPNCPYHQIIKLDASGRWRVNSECEPVAAIRFQ
                     KWFVLPPVMEYFYKSKNPFYRVLPPFRDDCVPQESGAGPDMIYPKNNSRIYIPVDLNG
                     EREKVVFKAAHNITGIKLHWYLDNTFIGSTSDFHQMALAPEAGKHKLTLVDQKGQHLT
                     VNFEIIEKQRQNKP"
     gene            complement(3546..>4102)
                     /locus_tag="PLI16_06895"
     CDS             complement(3546..>4102)
                     /locus_tag="PLI16_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH84325.1"
                     /translation="SSGCYDTSTVTIAVHPVPVITVDTIPPGCPGMNNGSITLTVNDG
                     TPPYVYWWNTGDSLSALSDLTNGTYSVTVSDARQCSASETIEFNILADCIDPVVYVPN
                     VFSPNGDGINDVVFVHGKDIFIDWIIFDRWGEEVFESKDISIGWDGTFKGKKMPSDVY
                     VYRLIVTWADGTEIRKKGNISLVR"
BASE COUNT         1204 a          944 c          903 g         1051 t
ORIGIN      
        1 cagtatccag gccgtatata ccttggtcag cctgtcgtat tgaaaatagg tcccgctggc
       61 attcgaccat gagtaagttg ctccggcatt gcacacgtta ttgatggtta cataatttgc
      121 cggattgttt ggcatctttg cgatatttac cgaactattg gaaaaagggc ctgaaatacc
      181 cggaccgctg acgaaaaatc caaaaacatc gttatagctc gaattgcaaa attcatcaaa
      241 ctcttcagag ccaaacacat actgaaattt tatagtatcg gctattggaa taaagtcaaa
      301 ttcaagtacg gctttgtcat acaccgtatt tgaaggcacc agtgcctgca ggtcgggatc
      361 gctgccagtt gcagagccgg ttccggaact tccggtatta ttcggcccaa tcgcattgga
      421 accctgccct gatgtgatga taattccctg tgcaaagcct aattgcgtag ctgcaacacc
      481 agcggcgtta aaactaccta tcatgttgga gctgatattg gcagcagaac cattaaaaag
      541 tccgttgctc acggtaacac cggttccaac caacacattc tgaactaatt gtatcggagt
      601 catgcccaac gctgtgccct gcaccaccgt tagttgcgca ggtaacctta aggatatcaa
      661 aaatcctccc gccaaaaaaa cgcccaacca aaacttaaag aaaatatttc taaacatatg
      721 ccatatatta cttttaaaaa aaccaagcca tatataatca gtatcttata tatgcaaagt
      781 tataaagaat tatcgatgta tacgtgattg cctcccaaaa agttatttct tattaaaaaa
      841 atgattaatc caaattataa cagattgttt atttttataa cggatttaga ggaaaaataa
      901 ttggggacag cgcacattac cggcaaaaaa tcttttggca ggtcatcatt tcaaaggttt
      961 ttaaaaaaga aatggtgggt attttttgca ttagcattca taattttttt ttggttttca
     1021 ttaccccgtc ccctattcag ggatccttgc tgcactgtga tggatgattg ccagggtgta
     1081 ttgctgggag cccgcatagc tgccgaccac caatggcgtt ttcctgcggg gaagacagtg
     1141 ccggaaaaat ttcagaaggc ggtaataatg tttgaggatc gtttttttta ttatcacaac
     1201 gggctcaatc cctgggctat ggccagagct gcttaccagg atatcaaagc cggaaaaatt
     1261 gtaagcggtg gcagcaccat caccatgcag gtaatacggc tttcaagaaa aggcaggcca
     1321 cgcactgttt ttgaaaaaat agcagaagct tttctggcgg taaggcttga actgacttac
     1381 agcaaagagg aaatacttgc tctatatgca gccaatgcgc cttttggtgg caatgtagtg
     1441 ggtattgatg cagcatcctg gcgttatttt ggcaaaaatg ccgcacagct atcatgggca
     1501 gaagcagcca ccctcgcggt tttgcctaac agtcctgcgc tgatttaccc cggaaaaagc
     1561 catgacaaac tgctgaagaa aagaaacaaa attctaaata aactctataa aaataattat
     1621 atcagccagg aagaattcct gcttgcacaa aacgagcccc tgccagggaa gccttatccc
     1681 ttgccgcagg cagcatcaca cctcctcgac cgctctataa aagaaggtta ttccggtaaa
     1741 agaataaaaa caactctgga tatcggcctg caaaccagag ttctatccat catcgaacga
     1801 taccacgaaa aatatagtgc taataaagtc aataatgccg cagtacttgt ggttgaggtt
     1861 gaaacgggca acgtattggc atatgtggga aataccaccg aaacaaacca tgacggtaat
     1921 ggttgccagg tggacatcat tacctctcca agaagcacgg gcagcatcct taagcctttt
     1981 ttatatgcgg ctatgatcaa cgacgggcaa ttgctgcctg ctgcactggt gccggatata
     2041 cctgttcagt tgggcggctt tattcctgaa aactacagcc tgacatacga tggtgcagtg
     2101 cctgcaaaaa gagcgttgtc gcgttcactc aacgtgccgg ctgtcaaaat gttgcaagca
     2161 tacggtatca accgttttaa ttatgtcttg aaaaaaatcg ggcttaccac gttacacaaa
     2221 cctccctctc attatgggtt ggccattatt ttgggcgggg ccgaagccaa cctgtgggat
     2281 atgacaggcg catacgccag tatggcgcgc acgctcaatc attatcagcc ttatgattcc
     2341 cgctacgata aaggagactt tcgcccgccg gtttatattt ttcaggatgc aaaaaagaaa
     2401 agcccgggca ccgaaaaatc ttctgtacta agtgcttcat ccatctggtt tacctttgag
     2461 gccatgaatg aagtttcccg gcctgatgaa gatatggaat ggcaacagtt ttcttcgtcg
     2521 gctaaaatag cctggaagac cggcaccagt tacggaagcc gcgacgcatg ggcaataggc
     2581 gtaaaccccc ggtatgtagt ggctgtttgg acaggaaatt ctgacggcga agggcgaccg
     2641 ggaatgaccg gcctgactta tgcggcaccc attatgtttg aagtgtttaa atctttgaag
     2701 ccggagggtt ggttctatgc gccatacgat gacatgatcc gcctgcctgt atgcaggcac
     2761 agcggattta aagcaacagc ggtttgcgaa gaggttgata cggtgtgggt gcagaaaaat
     2821 ggcgccaaaa gccccaattg cccgtaccat caaataataa aacttgatgc cagcgggcgg
     2881 tggcgcgtaa acagcgagtg cgaaccggta gctgcaatcc gttttcaaaa atggtttgtg
     2941 ctgccacccg tgatggagta tttttacaag tcaaaaaacc cattttaccg cgttttgcca
     3001 cccttccgcg acgattgtgt gccgcaggaa tcgggtgccg ggccggatat gatctaccct
     3061 aaaaacaaca gtcgcatcta tattcctgtg gaccttaacg gggaacgtga aaaggtagtt
     3121 tttaaagcgg ctcacaatat aacagggata aaactccatt ggtaccttga taatactttc
     3181 attggcagta ccagcgattt tcatcagatg gcgcttgcgc ccgaagccgg caagcacaaa
     3241 ctaaccctgg tggatcagaa aggacagcac ctgacggtga attttgaaat catcgaaaaa
     3301 caaagacaga acaaaccctg attatcccaa tttacgcatt aaatacttct attacgtagc
     3361 aatagggcag accaatcatt actgtctaat attcaataac atatttataa tcattaatgc
     3421 gtaatcgaca tagaaaatgt taccgacaag tcggtaacat tttctatgtc gggattaccc
     3481 agatgcaaca cacaatcccg cccactgctc aatagttcac tacgttccta ttgtgcaatc
     3541 tgggattatc tgaccagcga aatattgcct ttcttacgaa tttcagtgcc atcagcccag
     3601 gtcacgataa gccggtacac ataaacatcg gaaggcattt tttttccttt gaatgttccg
     3661 tcccacccta ttgaaatatc tttagattca aagacttctt cgccccaacg gtcgaaaata
     3721 atccagtcaa taaaaatatc tttaccgtga acaaaaacca catcattgat accatcgccg
     3781 ttgggcgaaa atacattagg cacataaaca accgggtcta tacagtctgc taaaatatta
     3841 aattcaatgg tttcagaagc agaacactgg cgggcatcgc tcaccgttac cgagtacgtt
     3901 ccgttggtaa gatcactgag ggctgacagg ctgtcgccgg tattccacca ataaacatag
     3961 ggaggggtcc cgtcattcac cgtaagagtt atggatccgt tattcatgcc ggggcatccc
     4021 ggtggtatgg tgtccacagt aattacaggt accggatgca ctgcgatggt cacggtactg
     4081 gtatcataac atccgcttga cg
//
LOCUS       DAOSFP010000209         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19904_length_4081_cov_4.616493, whole genome shotgun sequence.
ACCESSION   DAOSFP010000209 DAOSFP010000000
VERSION     DAOSFP010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19904_length_4081_cov_4.616493"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="PLI16_06900"
     CDS             complement(<1..557)
                     /locus_tag="PLI16_06900"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HOH84326.1"
                     /translation="MMKKLLILFFAVFFCRLNYAQTPSGNFLIGTGIADVTYPTFGMG
                     MQGMADDYQKTTEVETPLYARAFIIADASSQKRIAIIVFDMLSCNQVVKTKVIEALQN
                     DTTFHHLYNIDNVLISGTHTHSAPGGFSEYFLYNVTIGGFDQMCLDHIVKGIVEAMRK
                     AHKNLGPGKVYFNKGNLVDCGRNRS"
     gene            complement(559..2277)
                     /locus_tag="PLI16_06905"
     CDS             complement(559..2277)
                     /locus_tag="PLI16_06905"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84327.1"
                     /translation="MKNLLLVLFLFMISYLSKGQVLINESFDSTAFPPAGWSTRQVSG
                     EGSKSIWERVITGTDAVCGAHSGEGFTRFFGYNLKTGKSAILVSPPIDLSGTGSNSGT
                     ISLWMFRDNSYPNTYDSLDVYINDTSILSGAVHLGKIIRHKGQPPVEAITGWYRYSFM
                     IPASFNGTKNHIILKATSLFGYDIMIDDIMVKVSGPNDAGIFAITLPDSSAGTGNNPV
                     RVSIKNFGNASLNSTAVGWSVNGVACTPYVYLNSSGLATNAIDSSVIIGNYDFTTGGP
                     FIIKAWTQYPNSLNDIDYSNDTATKTVWINAYATLPFEENFDGVWINKKDSNDVPSIF
                     WNNTPVRGNNSWRRDDEGFTAGWISLTSGNFPPAGNDSNSHSARFHTFDAGPGTSGNL
                     DLCLDFTLQGYKALDFWHYNENGQDSLSVLLSENGGISYSPVVSLSSSTGWTHHYLSL
                     GSSTTPHAVVRFRATSDFGNTDIGVDSVRVLIDNTINTEIIENNSFVTVYPNPSNGLI
                     NIDIRNISDAELSIFSLSGQQVYSNSSSTANTVQQLDLTWLPRGAYVLQIKNDKNNII
                     RKLVIQ"
     gene            2741..>4081
                     /locus_tag="PLI16_06910"
     CDS             2741..>4081
                     /locus_tag="PLI16_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOH84328.1"
                     /translation="MKKFFIFFVICLFYSSATVLATDVSGTISSNTTWNLAGSPYVVT
                     SSITINHGVTLTIDPGVNVRFDDGQTIAVLGGLNATNVIFTSNNGSPTSGIWGNFQVG
                     NGNPADSGSVVLNSCQLLYGSQFVVASGTATLNGTDISNFTNHTVWIDNWGRFNMTGG
                     NINTNNAGAAAWGCAVFGIVWSHASLSGVTIQHYSNGVHLNPGATISISDMNITNCGW
                     PISFSDGASDFSVSGSNSFTGNGNNAVLINGMELSDTLVLPEINIPYYFPSGMQVNPA
                     GCLIVGSNNVLKFQDNTILNINGTLIAEASTGEGIYFTSFRDDNWGGDANNDGSATAP
                     ATGNWYGIRFSDPSHDANCLMRRCYVRYAGQDANGGITTENASPTIDQCDLSNNYYGI
                     CMRYASNPVFTNNTIGSSQMTPIAMSFEADPVMSNNTLSFSDNAYDAIGLLGGDL"
BASE COUNT         1137 a          836 c          929 g         1179 t
ORIGIN      
        1 ctggaacggt ttcttccgca atccaccagg ttgcctttgt taaaatatac ctttccgggg
       61 ccgaggtttt tatgtgcttt tctcatggct tccacaatgc cttttacgat atggtcaagg
      121 cacatctggt caaagcctcc gatagtaaca ttataaagaa aatattccga aaaacctccc
      181 ggggcgctgt gggtgtgcgt tccggaaatc agcacattat caatgttgta taaatgatga
      241 aaggtggtgt cgttttgcag ggcttcaata acctttgttt tgaccacctg attacaggaa
      301 agcatatcga acacgatgat ggctatgcgt ttctgactac tggcatcggc aataatgaat
      361 gctctggcat acaggggcgt ttccacttct gtggttttct gatagtcgtc ggccataccc
      421 tgcatgccca tcccgaaagt aggatacgtt acatcggcta tcccggttcc tataagaaaa
      481 tttcctgatg gggtctgagc ataattcaac cggcagaaaa agacggcaaa aaatagtatt
      541 aaaagttttt tcatcatatt attgaataac taatttgcgg ataatgttat tcttgtcatt
      601 ttttatctga agaacataag ctccccgtgg cagccaggtc aaatcaagtt gctgcacagt
      661 attagccgtg gaggagctat ttgagtacac ttgttgcccc gataatgaaa atatcgaaag
      721 ttcagcatca gaaatatttc tgatatcaat attgatcagt ccgtttgacg ggttgggata
      781 gacagttaca aaagaattgt tttcgataat ttcggtgttt atagtgttgt ctatcaaaac
      841 gcggacacta tccactccta tgtcggtatt gccgaaatca gatgttgccc tgaacctgac
      901 tacagcgtga ggcgttgtgg aggatcccag agaaaggtaa tggtgtgtcc agcctgtgga
      961 ggaagaaagt gaaaccacgg ggctgtagct aatccccccg ttttcagaaa gcaacaccga
     1021 caaagaatcc tggccgtttt cattatagtg ccagaaatcc agtgccttgt agccttgaag
     1081 cgtaaaatca aggcataggt ctaaattacc cgatgtgccc gggccggcat caaaagtgtg
     1141 aaaccttgct gaatgtgagt tgctgtcatt cccggcaggc gggaagtttc ctgaagtcag
     1201 actaatccat ccggcggtaa aaccctcatc atcgcggcgc caggagttgt tgccgcgcac
     1261 cggagtatta ttccaaaaga ttgacggcac atcattagaa tcttttttat tgatccagac
     1321 tccgtcaaaa ttttcttcga aaggcagggt tgcatacgca ttaatccaaa cggtttttgt
     1381 ggctgtatcg ttggagtagt caatgtcatt aagggaattg gggtattgtg tccaggcttt
     1441 tatgataaag ggacctccgg tggtgaaatc gtaatttccg ataataacgg aggaatcgat
     1501 tgcatttgtt gccaggccgc tgctgtttaa atatacatag ggggtacatg cgacaccatt
     1561 aacggaccat cctaccgctg tggaattcag ggatgcattt ccgaaatttt ttatgctgac
     1621 ccttacagga ttatttcctg taccagctga tgaatcgggg agggtgatgg caaaaatacc
     1681 cgcatcgtta ggcccggaaa ccttaaccat tatgtcatca atcataatat catagccaaa
     1741 aaggcttgta gcttttaaaa tgatatggtt ttttgttccg ttaaaagatg ccggaatcat
     1801 aaaagaatag cgataccatc ccgtgatggc ttcaaccggc ggttgcccct tgtggcggat
     1861 gatctttccc agatgaacgg ctcctgaaag tatcgaagtg tcgttgatgt aaacatcaag
     1921 gctgtcgtat gtattagggt aactgttatc acggaacatc cacagtgaaa tcgttccgga
     1981 atttgatcct gtccccgaca ggtcaattgg tggtgaaaca aggattgcgg attttcctgt
     2041 tttgaggtta tatccgaaga atcttgtaaa gccttcgccg gagtgcgccc cacaaaccgc
     2101 atcagttccg gtaattactc tttcccatat tgatttagaa ccctcgccgc ttacctggcg
     2161 cgttgaccat cctgcaggcg gaaatgccgt agaatcaaaa gattcattta tgagaacctg
     2221 tccctttgac agataagaaa tcatgaataa aaacaaaacc agtagtaagt ttttcatttg
     2281 agaaatattt ctgagcctta ttgttttggg acaaaattat gacttttttc tgtaaaaagg
     2341 gcttttcgtg tataagagga ggtgggagag aggctgtgtt ttaagtccta tgcaacgttg
     2401 tgtactccta aaagtctgct gcttcggttt tttaggttaa aaactgacag attttgattt
     2461 ttgagaccac cttccaaaaa ggaaggatgt taggtacaac gcctgacccg agattaaaac
     2521 cgtcgcaaca cattaaacat attcaaatcg aattaaaaag tgaagcatgt caatgtgtta
     2581 aaaatcaaaa agtaaaacac ggtaaatgtt aatgtcggaa aaatatttta atttttaaaa
     2641 aaaatggaaa aaattgagtg tatatttaca gtctttaaat aaaattattc ttaaccatat
     2701 taaagtgcgg agtcagcact ataatcactg tcgtaacatt atgaagaaat tttttatttt
     2761 ttttgtaata tgtctttttt acagttcagc gactgtcctg gcaacagatg tttcaggtac
     2821 gatttcgtcc aataccacat ggaaccttgc cggaagcccg tatgtggtaa cctcctccat
     2881 aaccattaat catggagtaa ctcttaccat tgacccgggc gttaacgtga ggtttgatga
     2941 cggacaaacc atagctgttc ttggaggatt gaatgctaca aatgtaattt ttacttccaa
     3001 taacggcagt cctacgtctg gcatatgggg caattttcag gttggcaacg gtaatccggc
     3061 tgattccggg agtgtcgtac taaactcctg ccagttgttg tacggatcgc agtttgttgt
     3121 ggccagtggc actgctactc tgaacggtac tgatatcagt aattttacca accatactgt
     3181 ctggatagat aattggggac gctttaatat gaccggggga aatatcaata ctaacaatgc
     3241 cggagccgca gcctggggtt gtgctgtttt tggcattgta tggtcacatg ccagtctttc
     3301 gggggtgact atacagcatt acagcaatgg agtgcatttg aatcccgggg ccaccatcag
     3361 tatttcagat atgaatatca caaattgcgg atggcccata tcttttagcg atggcgcttc
     3421 tgacttttcg gtaagcggtt caaattcgtt taccggaaac gggaataatg ccgtgcttat
     3481 caatggcatg gaactttcgg acacactggt cctgccggaa atcaatattc cgtattattt
     3541 cccaagtggc atgcaggtta atcccgccgg atgcctgatt gtcggatcaa ataatgtact
     3601 gaaattccag gataatacta ttctgaatat caatggaacc cttattgctg aagccagtac
     3661 aggggaaggc atctatttta cttcttttcg ggatgataac tggggcgggg acgccaataa
     3721 cgatggttcg gctacagcac cggcaacggg aaattggtat ggaatacgtt tcagcgaccc
     3781 cagccatgat gctaattgtc tgatgcgccg ttgttatgtg cgttatgccg gacaagatgc
     3841 caatggaggg ataaccacag aaaacgcaag ccctactatt gatcagtgcg atctgtccaa
     3901 taactactat ggaatttgca tgagatatgc gtcaaatcct gtttttacca acaataccat
     3961 aggttccagc cagatgacac ccatagctat gtcgtttgaa gccgacccgg ttatgtcaaa
     4021 caatacgttg tcattttcgg ataatgcata cgacgccata ggtcttcttg gcggtgacct
     4081 t
//
LOCUS       DAOSFP010000210         4077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19932_length_4077_cov_3.886872, whole genome shotgun sequence.
ACCESSION   DAOSFP010000210 DAOSFP010000000
VERSION     DAOSFP010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19932_length_4077_cov_3.886872"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1369
                     /locus_tag="PLI16_06915"
     CDS             152..1369
                     /locus_tag="PLI16_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOH84329.1"
                     /translation="MNKESLLFTPSNIGPLTLRNRSIRAAAFEGMCPGNRPSKMLIDY
                     HRSVAAGGIGMTTVAYASVTRDGLSFPHQLWLRKEIIPELRTLTDAIHCEGAAASIQL
                     GHCGNMSKSAVAGRMPLSPSGGLNIYSPTWSKKMSKEEIARIAAAYGEAVNIARESGF
                     DAVEVHAGHGYLISQFLSPYTNHRKDEFGGSLENRMRFMQMAMRSVISAAGNDMGVVV
                     KMNMNDGFRGGMQTDECLEVARTLEQEGAHALVLSGGFVSRAPMYVMRGSMPIKTLTY
                     YMKNIPLKLGVKIAGRWMIRPVPFREAYFLEDALKFRKEVKLPLVYVGGLISGKKIDE
                     VLDSGFEFVSMARALLNEPDFINRLKNDRQAVNQCVCANYCIARMYSIEMACHQHRSD
                     VPKCLHKELGIKK"
     gene            1382..2185
                     /locus_tag="PLI16_06920"
     CDS             1382..2185
                     /locus_tag="PLI16_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH84330.1"
                     /translation="MGLTLQETVLITGGSSGIGYAIAREFASRGYKLILVSNQQEQLK
                     KVAAEFNEKFNTETRTLFLDLAAPDAAEKLYAWCGQQNLTVDILVNNAGMLIFREIVE
                     TGMEKTKNILALHTTTPALICTLFAKDMKQKRKGNIVIISSLAAYMPYPGIALYAATK
                     RFLKSFARSLRTEMLDYQVNVTCVCPGAVSTDLYNLSQRWRNIAINTGIMMRPEKLAR
                     KIFRAMQRRKALLIPGWVNKVALPVLGILPHGLIVLIRRHSPLLPPEKQ"
     gene            complement(2232..2396)
                     /locus_tag="PLI16_06925"
     CDS             complement(2232..2396)
                     /locus_tag="PLI16_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84331.1"
                     /translation="MLYTVLIDSKLEDLIKQVNDLIQKGWIPQGGFCESSSGAIYHYT
                     QAMIKNSKTQ"
     gene            2671..>4077
                     /locus_tag="PLI16_06930"
     CDS             2671..>4077
                     /locus_tag="PLI16_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84332.1"
                     /translation="MRKTAIIIVIFLILSYPSAAQKDGYKAMYDSAMTYFSLQNYQKA
                     LPVFLRMYNARPDNANVAYHIGASYLHMPSEKYQAIPYLEKACQKASKTFLQNMEEQK
                     APVSAYFELGTAYLINYDLAKSIEATTKYKTFFDANEKNDQTFLREADRQIAMCSVAR
                     EMLKNPVPIKVENLKGKINTIFPEYAPVVDNSGNTLFFTARRNTNMGEMIEDNGYYFE
                     DVYYAKKDQKGNWEEAINAGDKVNTYDHEASISISPDNKQLFIYKDDGGDGNIYISNF
                     ADNEWSVPVKMPSPVNSKAWETHASLSHDGNTLYFTSDREGGLGGLDIYKATRQANGK
                     WGQVENLGDTINTEYNEEGPFILANNTLYFCSEGHNTMGGYDIFYSKRTKEGKWTIPV
                     NMGYPINTTDNDMFYTPVDSVTAYFASVRFVDDFDVKGFGNLDIYKLTIHNLKIRGLA
                     LDKKTNNPVPGALVFLMND"
BASE COUNT         1199 a          925 c          927 g         1026 t
ORIGIN      
        1 tattcatatt taaacaaaag aggctgtttc gattaggaga cagccttttt ttttgcacgg
       61 gccgtactat attcgggcct gccgtcattt tttgcaaagg cacaaaataa aatatcatta
      121 attttggggc tgttttataa atataacccc agtgaataaa gaatccttac tgtttactcc
      181 ctccaatatt ggcccgctga ccttgcgaaa caggagcata cgtgctgccg cctttgaggg
      241 catgtgcccg ggcaaccgtc cgtcgaagat gctgattgat taccatcgtt cggtggccgc
      301 cggcggcatc ggcatgacca cggtggccta tgcttcggta acccgcgacg gcttgtcgtt
      361 tccgcaccag ttgtggttgc gtaaagaaat cattcctgaa ctgcgtactc ttactgatgc
      421 catccactgc gaaggcgcag ccgcctccat acaactggga cattgtggca acatgtcaaa
      481 aagtgctgta gcaggcagaa tgccgctgtc tccctctggc ggactaaaca tttattcgcc
      541 cacctggtcg aaaaaaatga gcaaagaaga gattgcgcgg attgcagcag cttacggcga
      601 agctgtaaac atcgcccgcg agtcgggatt cgatgcggtg gaggtacatg ccggccacgg
      661 atacctcatc agccagtttc tttcacccta taccaaccac cgcaaggacg agttcggagg
      721 gtcgctcgaa aaccgaatgc ggttcatgca gatggcgatg agaagtgtaa tcagcgcggc
      781 cggcaatgat atgggtgtag tcgttaaaat gaatatgaac gatggttttc gcggcgggat
      841 gcaaacggac gaatgtctgg aagtggctcg caccctggag caggaaggtg cccatgccct
      901 ggtactgagc ggcggttttg tgagccgggc gcccatgtac gtgatgaggg gaagtatgcc
      961 aataaaaacg ttgacctatt acatgaaaaa tatcccgctg aagttgggtg tgaaaatagc
     1021 cggacggtgg atgatacggc cggttccgtt ccgggaagcc tattttctgg aagatgccct
     1081 gaaattcagg aaggaggtaa aactgccgtt ggtttatgtg ggcggactga tttccggaaa
     1141 gaaaatcgat gaggtgctgg attccggctt tgaatttgta tccatggcgc gcgccctgct
     1201 gaacgagccg gattttatca accgcctgaa aaacgacagg caggccgtca accagtgtgt
     1261 atgcgccaac tattgcattg cccgcatgta ttcaattgaa atggcctgtc atcagcatcg
     1321 cagcgatgtg cccaaatgcc tgcacaaaga acttggcatt aaaaaataat tcactttgat
     1381 tatgggcttg acattacagg aaacagtgct gattacaggc ggaagctccg gcatcggcta
     1441 tgccattgcc agggaattcg cctcgcgggg atataagctg atactggtga gcaatcagca
     1501 ggaacaactg aaaaaggttg ccgcagaatt caatgaaaaa tttaatacag agacacgtac
     1561 actattcctc gatctggcgg ctcccgatgc cgctgaaaag ctttacgcat ggtgtggcca
     1621 acaaaacctt acggttgata ttctggtaaa caatgccggt atgttgattt ttcgcgaaat
     1681 tgtggaaacc ggtatggaaa aaacaaagaa catcctggcc ttgcacacaa caacgccagc
     1741 actgatctgc accctatttg ccaaagacat gaaacaaaag cgcaagggca acatcgttat
     1801 catttcatcg ctcgccgctt atatgcctta tcccggcata gcgttgtatg cagccaccaa
     1861 gcgttttctc aaaagtttcg cccgttcgct gcgtactgag atgctggatt accaggtaaa
     1921 tgttacctgt gtatgtcccg gagccgtttc taccgacctg tacaacctaa gccaacgatg
     1981 gcgcaatatt gctattaaca cgggcatcat gatgcgtccc gaaaaactcg cccggaaaat
     2041 tttccgcgct atgcagcgcc gaaaagccct gctgataccc ggctgggtaa acaaggtggc
     2101 attgccggtg cttggcatac tcccgcacgg ccttattgtg ctgataagac ggcattcccc
     2161 cctcctgcct cccgaaaaac aataaatttt aaggcctaat accaggtacc cctaaattat
     2221 cttttcaggt tttactgggt ttttgagttt ttaatcatgg cctgggtata atgataaatc
     2281 gccccactgc tgctctcgca aaaacctccc tgaggtatcc agcctttttg aatcagatcg
     2341 tttacctgct taatcaggtc ttctaatttc gaatcgataa gaaccgtgta taacataaaa
     2401 tacaattttt gattttatta attttgacta ttttcgtcat aaagttatga tttttttaca
     2461 aaaaaaccgg gtgcatgttt tataattcaa aaaaatattc aggaagggct gaaaataaac
     2521 aaaagtccct ttagtaacag aaatgtctga aacagataac aatttctaaa tcttaaaaaa
     2581 acgtcagatt aacaaaaatg tttatattaa aaaaaatgag tatttttgga tagtgaaaat
     2641 tatttttaag accaaataaa aaaataagat atgagaaaaa ccgctattat tattgtgatt
     2701 tttttgattc taagttatcc ttcagcagcc caaaaagacg gctataaagc catgtatgat
     2761 tcggctatga catatttttc ccttcaaaat tatcaaaaag ccctgcctgt ttttttacgg
     2821 atgtacaatg cccggcccga caatgccaat gtggcctatc atataggcgc ttcctacctg
     2881 cacatgccgt cagaaaagta tcaggcgata ccctacctgg aaaaagcctg ccaaaaggca
     2941 tccaaaactt ttttgcaaaa tatggaagaa caaaaggcgc cggtcagcgc ctattttgag
     3001 ctgggcactg cctatctgat taattacgac ctggccaaat ccatagaagc aaccactaaa
     3061 tacaagactt ttttcgatgc caatgaaaaa aatgaccaga catttcttag ggaagctgac
     3121 cgccagatag ccatgtgcag cgtggcgcgg gaaatgctga aaaatcccgt acctattaaa
     3181 gtagagaacc ttaagggtaa aatcaacact atattccccg aatatgctcc ggtggtggac
     3241 aattcgggta acaccctctt ttttactgct cgccgtaata ccaatatggg ggaaatgata
     3301 gaagataacg gctattattt tgaggatgtg tattatgcca aaaaagacca gaaaggtaac
     3361 tgggaggaag ctataaatgc gggtgacaag gtaaacacat acgaccatga agcctccata
     3421 agcatctcgc ccgacaacaa acaattattc atctataaag acgatggcgg cgacgggaac
     3481 atatacatca gcaactttgc tgataacgaa tggtcagtgc ctgtaaagat gccttcacct
     3541 gtcaacagca aggcctggga aacgcatgcc agcctttcac acgatggcaa caccctgtat
     3601 tttaccagcg acagagaagg cggcctgggc ggcctcgata tatacaaggc tacacggcaa
     3661 gccaacggca aatggggaca ggtagaaaat ctcggcgata ctattaatac tgaatataat
     3721 gaagaaggcc cctttattct ggcgaataat actttatatt tctgttctga aggacataat
     3781 accatgggcg gctatgacat tttttacagc aaacgtacaa aggaaggcaa atggactata
     3841 cctgtcaaca tgggataccc aatcaacaca acagataacg acatgtttta tacaccggta
     3901 gattcggtaa ccgcttattt tgcgtcagtc cggtttgtgg acgatttcga tgtcaaggga
     3961 tttggcaacc tggatattta taaattaacc attcataatc tgaaaatcag aggactggca
     4021 cttgataaaa aaaccaacaa ccctgttccg ggcgccctgg tgttcctgat gaacgat
//
LOCUS       DAOSFP010000211         4071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_19982_length_4071_cov_4.332171, whole genome shotgun sequence.
ACCESSION   DAOSFP010000211 DAOSFP010000000
VERSION     DAOSFP010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19982_length_4071_cov_4.332171"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..927)
                     /locus_tag="PLI16_06935"
     CDS             complement(103..927)
                     /locus_tag="PLI16_06935"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOH84333.1"
                     /translation="MKKQIFLSFALTLLSFTINAQYYHYYGDTVRLALPVYNGNLQWQ
                     QTSDTTAVWVDIPGATYQPYEIAVASSNYYRARVINGSCNPVFSQVKAVRLYNVQQRL
                     DHGETPKHIFDTGIPTDSLYGKTYQGGLIFYLNTINGNGLVAAPFDQSTGAEWGCHGT
                     AISGADGTVIGTGAQNTLDIEASCATVGSAADICANLILGAYSDWFLPSRDELHEMYN
                     NLKVKGFGGFSNNYYWSSSECATYELNDAWLQNFQYGNQYNYVKYNPTYVRAVRAF"
     gene            complement(1602..2252)
                     /locus_tag="PLI16_06940"
     CDS             complement(1602..2252)
                     /locus_tag="PLI16_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH84334.1"
                     /translation="MCIKSRISYKLQQFIINTQTLNLKSAPLFRRILAFIYKILGRKL
                     ITKSMISSTHCKGCSVCAQVCPNHAIKFRLRNPYRNRHCQGCLVCAYFCPQRAVELPL
                     ASLFGAFLLIWLPYDEWIINLFVPEYFSITKSFGYSLFSFILWCVAYVVVVFIYDKVM
                     FLLSTLAISKKIAKISVVKKARNYIHPGSIFPVLMPRYTQPVYDKNMNMSVRPDKE"
     gene            complement(2284..3525)
                     /locus_tag="PLI16_06945"
     CDS             complement(2284..3525)
                     /locus_tag="PLI16_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH84335.1"
                     /translation="MIEEQKTKTHDEKRIFGFSRNIFFTGIISFLTDTSVKMLYSVMP
                     MFLMSIGASKTSLALIEGIAESTASLIKALSGFWSDKIGKNKPFLLLGYGLSALIIPL
                     YVFVFLPVHVLVLRFIERFGKGIRTAPRDSLIAGSVINGETGKSFGLQKAMDNSGAIL
                     GPLIAFGLLWLFPGNFRVIFLLAGIPAILAIFVIIFGIREAKKNKSELFTKFRFRDFS
                     PRFYFFLVIIFVFTLGNSTDALLMVKANDVGIKVAFIPLVYLITNLVSVLFAIPIGKL
                     SDRIGKEKILITGFLIYAAVYFGFGLSGSIGTIISLFAFYGLYSACTDGIQKAFVADL
                     TDKNKKGTGLGIYNALLGITLLPASLIAGVLYDKVDSSVPFYFGAAMAIISAIMMVFF
                     VLKYKIHVTGNPKFEKNSRDF"
     gene            complement(3884..>4071)
                     /locus_tag="PLI16_06950"
     CDS             complement(3884..>4071)
                     /locus_tag="PLI16_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84336.1"
                     /translation="FSVGTNEKRTYKVLLNASYLGRFYLPTVSCAAMYDDAIYARTKG
                     QWVEVVPYNGLATADNK"
BASE COUNT         1300 a          830 c          739 g         1202 t
ORIGIN      
        1 tattgttttt attgtacttt taaaagatta aatattttta aacgcatcac tactatttca
       61 tgattataat ttttttacaa cagaattatt ctaatggata aattaaaaag ccctcacagc
      121 cctcacgtag gttgggttgt acttaacata gttatactga ttgccatatt ggaaattctg
      181 aagccaggca tcgtttaact cgtatgtggc gcactcactc gaactccaat aatagttatt
      241 ggagaaaccg ccaaagcctt taacttttaa gttattgtac atttcatgaa gttcatctct
      301 tgatggcaaa aaccagtcgg aataggcgcc taaaatcaga ttggcgcata tatctgcggc
      361 tgaacccact gtagcacaac tggcctcaat atcgagagtg ttctgtgctc ctgttccaat
      421 gactgtccca tctgcccctg aaattgctgt tccatggcaa ccccattcag ctccggtact
      481 ttgatcaaag ggtgccgcaa caagaccatt cccattaatc gtgtttaaat aaaaaatcag
      541 tcctccctgg tacgttttgc cataaagcga atccgtggga attccggtat caaatatatg
      601 ctttggtgtt tccccatggt ctaagcgttg ttgaacatta tacagtctga cagctttaac
      661 ttgcgagaat accgggttgc atgagccatt aattaccctg gcacgataat aatttgatga
      721 agcgacggca atttcataag gctgataggt agctcccgga atatctaccc atacggcagt
      781 ggtgtcggaa gtttgttgcc attgcaaatt gccgttgtaa acgggcaacg caagtcttac
      841 agtatcaccg taataatggt aatactgtgc gttaatagta aagcttaaaa gcgtcaacgc
      901 gaatgataga aaaatttgtt ttttcattat ttttatatta tataatttta tatgtaagac
      961 tttctttatt tataaaaatt atcactcccg ggttttattc ttttttttaa actatgaata
     1021 aaaagggagt ctatttcgta aatatcctta caattcatta tgattattta ttttataatt
     1081 gaatttttat gtaaagcctc agcatttaat ttcgacttac gaatagtcaa ctatataaat
     1141 acaaatttat tgatattcat ttattgtttg tataaatatt taaattaatt ttttatcgtt
     1201 ataaaatcaa gaataagcgt acggttttag gtaatgaaag cttttcttgc caattgtttt
     1261 ttgttaaaaa aaagttcatt gtaatataaa tttttcatga aaaagaaaat ttttatatat
     1321 attttaataa aacaagctgg caaatcacga cgaaccaata atcttgatat ttgatatctt
     1381 tcaaatacat gaaggaacaa taaaacagaa gtagagcacc agaaccgaaa aagaatctgt
     1441 ttataaactt ctggaaaaga ataaaaaccc cagaaactcc gctgcaggcc cctgcccttc
     1501 ccgggcgact tcgacataat actgataacc aacgaatatt taaagaaaac cgtgatggat
     1561 gtggtcaaca ataagtagta tctgataaaa taacacctct tttattcttt gtccggcctc
     1621 acagacatat tcatgttttt gtcatacacc ggttgggtgt agcgcggcat aagtaccgga
     1681 aaaatcgaac ccggatggat atagtttcgt gcttttttaa cgacagaaat tttcgcaatt
     1741 tttttcgaga tagctagtgt gcttaaaaga aacattactt tgtcataaat aaaaacaacc
     1801 accacataag ccacgcacca caggatgaat gaaaacaatg aatagccgaa gcttttcgtg
     1861 atggagaaat attcaggaac gaacaaatta atgatccact catcgtatgg aagccatatc
     1921 agcaaaaacg ctccaaagag actggccagc ggtaattcca cagcccgctg cgggcaaaag
     1981 taagcacaca ccaggcatcc ttgacaatgc ctgttgcggt aaggatttct caaacggaat
     2041 tttatggcgt gattaggaca aacctgtgca catacggaac aacccttgca atgcgtggag
     2101 ctgatcattg attttgtaat aagctttctt cccagtatct tgtatatgaa tgcaaggatt
     2161 ctcctgaata agggagcgct ctttaaattc agcgtctgcg tgttgatgat gaattgctga
     2221 agtttataac ttatgcgact tttgatgcac ataatgcctg aggatcttta aaaaactaat
     2281 taatcaaaaa tccctgctgt ttttttcaaa tttcgggttg cccgttacat gaatcttata
     2341 tttaaggaca aaaaacacca tcatgatggc agatattatt gccatagcag caccaaaata
     2401 aaaaggtacc gacgaatcca ctttatcata aagcactccg gcaatcaggc tggccggcag
     2461 cagcgtaata ccaagcaagg cattgtatat cccaagtccg gtgccttttt tattcttatc
     2521 tgtcaaatcg gcaacaaatg ccttttgaat tccgtcggta caagcagaat acaatccgta
     2581 aaaggcaaat aacgaaataa tagttcctat gctgcccgag aggccgaaac caaaatatac
     2641 cgctgcataa atcagaaaac ccgtgatgag gattttttct ttcccaatcc tgtctgaaag
     2701 ttttccgata ggaatagcaa agagaacaga aaccaggttg gtaatgagat aaaccagggg
     2761 aataaaagca acctttatcc cgacatcgtt ggcctttacc attagcaaag catcggttga
     2821 attcccaagg gtaaagacaa atataataac cagaaaaaaa taaaaccttg gtgaaaaatc
     2881 acggaaacga aattttgtga acaattccga cttattcttc ttggcttccc tgatgccgaa
     2941 tatgattaca aaaatagcca gtatggccgg tatccctgcc agaaggaaaa taaccctgaa
     3001 attacccggg aacaaccata acagaccgaa agcaatcagt ggaccaagta tagcaccgct
     3061 attgtccata gctttctgca atccgaaact ttttccggtt tccccgttta tcaccgaacc
     3121 cgctatcagg ctgtcgcgcg gggccgtgcg gatgcctttt ccgaaccttt cgataaaacg
     3181 gagtacaagg acatgtacgg gcaaaaaaac aaatacatac aagggtatta taagcgcaga
     3241 aaggccgtag cccaaaagca gaaagggttt gttttttccg atcttgtcgc tccagaagcc
     3301 cgacagagcc ttgatgagtg aagcggtgct ttcagctata ccttctataa gggccagact
     3361 ggtttttgaa gcacctattg acatcagaaa cataggcatc acggagtaca gcatctttac
     3421 ggaggtatca gtcagaaaac tgataatacc tgtaaaaaag atattcctac taaagccgaa
     3481 tatccttttt tcatcatgcg tttttgtttt ttgctcttct atcataacaa tttcagcaaa
     3541 tgtgatgcaa aattatggta tatttttaca acactagcaa gactatgatt tgattttgag
     3601 aaataatgac aggtacggtt tttagcacat cgatgcatca ttgatacttc tgtctcctgc
     3661 tgctttctgt tacgaaaccc cattatccca aattccaaca tatcaagaag atacaaaata
     3721 tttaccgaat ttttgcggca aataagaaag attctcagtt ttgtctctgt tcttcaaatt
     3781 ctcatatacc ctcagtataa tacggttgtt cagaacttcg accacctcga acttttttga
     3841 ttttgtgagc tagccaacca acattattag agtcacccac cttttatttg ttatctgccg
     3901 tagcaagtcc attatacggc accacttcca cccactgtcc tttggtacgg gcatagatgg
     3961 catcatcata catggcggca caactcacgg taggcagata aaaacggccc aggtaactgg
     4021 cgttcagcag gaccttatac gttcgttttt cgttggttcc cacactgaag a
//
LOCUS       DAOSFP010000212         4043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_20172_length_4043_cov_4.935807, whole genome shotgun sequence.
ACCESSION   DAOSFP010000212 DAOSFP010000000
VERSION     DAOSFP010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20172_length_4043_cov_4.935807"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2600)
                     /gene="secA"
                     /locus_tag="PLI16_06955"
     CDS             complement(<1..2600)
                     /gene="secA"
                     /locus_tag="PLI16_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOH84337.1"
                     /translation="MANFLKLFLGTKSDRDIKSLQPILQKCLEVYPAISQLSNDGLRA
                     KTMEFRERIYEYVHEDEEQMAMLKEKAENDESVSIDDKEEIYLEIDRLEKKVYEKTQE
                     ILNDILPEAFAVVKETAKRFADHDKVEVTATAMDGDLAARFESINIVGDKAYYDSAWM
                     AGGTKIKWDMIHYDVQLIGGMVLHQGKISEMATGEGKTLVATLPVYLNALPGKGVHIV
                     TVNDYLARRDSEWMGMLFLFHGLTVDCIDKHEPNSESRKKAYLADITYGTNNEFGFDY
                     LRDNMTRSPEELVQRKHNYAIVDEVDSVLIDDARTPLIISGPTPKGENQLFENFRPNV
                     QKVYNAQKNLVTNMLAEAKKLLSQEPLSSENEKQGGVLLLRAHHGLPKNKALIKFLSE
                     PGMRALMQKTENFYMQEQSKNMHIIDDELFFVIDEKNNSVDLTEKGIDLITSGTDDAR
                     FFILPDIGNEIAELEKQNLSDNERLVRKNEMLQEYEVKSERVHTVTQLLKAYSLFEID
                     VDYVIIDNKIKIVDEQTGRIMEGRRWSDGLHQAVEAKENVKIEAATQTYATITLQNYF
                     RMYKKLAGMTGTAETEAGEFWNIYKLDVVVIPTNRPVIRKDFQDLVYKTKREKYNAVI
                     AEIVDLVEHGRPVLVGTTSVEISELLSKMLNLRKIRHNVLNAKLHQKEADIVAEAGQA
                     GTVTIATNMAGRGTDIKLGPGVREAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGTS
                     QFFVSLEDDLMRLFGSERIARVMDTLGLKEGEVIQHRMITKSIERAQIKVEENNFGIR
                     KRLLEYDDVMNKQREVIYLKRRHGLYGDRLAVDIADMFYDVSQRIVLEHQEERDFENF
                     KLDLLRNFGV"
     gene            2771..3118
                     /gene="rpsF"
                     /locus_tag="PLI16_06960"
     CDS             2771..3118
                     /gene="rpsF"
                     /locus_tag="PLI16_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOH84338.1"
                     /translation="MTKHYETVFILTPVLSEDQVKEAVKKYHDFLSEKKAEIVVSESW
                     GLRKLAYPIQKKSSGFYQLIEFKAEPNVIADLEVAYKRDERLIRFLTISLDKHAVAYR
                     ERKRAEKQNTEKA"
     gene            3149..3418
                     /gene="rpsR"
                     /locus_tag="PLI16_06965"
     CDS             3149..3418
                     /gene="rpsR"
                     /locus_tag="PLI16_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOH84339.1"
                     /translation="MAQTDSEIRYLTPINVDVKKKKYCRFKKHGIKFIDYKDADFLLK
                     FINEQGKILPRRLTGTSLKYQRKLAKAIKRARHLALLPYVADLLK"
     gene            3431..3880
                     /gene="rplI"
                     /locus_tag="PLI16_06970"
     CDS             3431..3880
                     /gene="rplI"
                     /locus_tag="PLI16_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOH84340.1"
                     /translation="MKVILKQDVTNLGYANEIVKVKNGYARNYLIPKGIAIMLTPSSE
                     KEHAELMKQRTFKEEKIRKESETLAKALENIVVKVGAKAGTSGKIFGSVNAIQLADAI
                     KQQFNYEIDRKKIVVDGESVKELGTYSAKINIYKDIKVDIKFEVIAE"
BASE COUNT         1176 a          910 c          783 g         1174 t
ORIGIN      
        1 tccacaccaa agttgcggag cagatcgagt ttgaagtttt cgaaatcgcg ttcttcctga
       61 tgttccaaca caatgcgctg agaaacatca taaaacatat ccgctatatc gacggccagg
      121 cggtcgccat ataatccgtg acgtcgtttc agataaataa cctcacgctg tttgttcata
      181 acgtcatcgt attccagaag ccgtttacgg atgccgaaat tgttttcctc cacttttatc
      241 tgggcgcgtt caattgattt tgtaatcatc ctgtgctgga tgacttctcc ttctttcaga
      301 cccagtgtat ccataacgcg ggcgatacgt tccgatccga acaggcgcat caggtcatct
      361 tcgagcgaaa caaaaaactg tgaagttccc gggtcgccct ggcgtccggc tctgcctcgc
      421 agctgccggt ccacacggcg ggactcatga cgttcggtgc ctataatggc caatcctccg
      481 gcttccctta cgccgggacc cagcttgatg tcagtaccac gaccggccat attggtagca
      541 atggtaacag ttcccgcctg ccctgcttca gcaacaatat cggcttcttt ttgatgcaac
      601 ttggcgttga gcacattatg cctgattttg cgcagattaa gcattttgct gagcaactcc
      661 gaaatttcta cggaggtagt acctaccagc acaggacggc catgttccac aagatctact
      721 atttccgcta tgacggcatt gtatttttca cgctttgttt tgtacaccag atcctgaaag
      781 tccttacgga ttacaggtct attggtagga atcaccacca catcgagctt ataaatattc
      841 cagaactctc cggcttcggt ttcggcagta ccggtcatac ccgccagctt tttatacata
      901 cggaaatagt tctgcagggt gatggtagca taagtttggg tagccgcttc aatttttaca
      961 ttttctttgg cttccacggc ctgatgcagt ccgtcggacc aacgccggcc ttccataata
     1021 cgtccggttt gctcatcaac aatttttatt ttgttatcaa tgataacata atccacatct
     1081 atttcgaaca gcgaataggc tttgagcagc tgtgttaccg tatgtacacg ctctgatttg
     1141 acttcatact cctgcagcat ttcattttta cgcaccaggc gttcattgtc cgacaggttt
     1201 tgcttttcca gttcggcaat ttcattacca atatcaggca aaataaaaaa acgagcatca
     1261 tcggtgccag acgtgatcag gtcgatacct ttttccgtaa ggtccactga attatttttt
     1321 tcgtcaataa caaaaaaaag ctcatcatca atgatgtgca tatttttgct ttgctcctgc
     1381 atataaaaat tctcggtttt ctgcatcagg gcgcgcattc ccggttcgct gagaaattta
     1441 attagggctt tgtttttcgg caatccatga tgggcacgca gcaacaacac gccgccctgt
     1501 ttttcgtttt ctgagctgag aggttcctgc gagagaagtt ttttggcttc cgccagcatg
     1561 ttggttacaa ggtttttttg agcattatat accttttgca cgttgggacg gaaattttca
     1621 aaaagctggt tttccccttt aggcgtaggc cctgaaataa taagtggagt acgggcatca
     1681 tcaatcaaca ccgagtccac ctcatccacg atggcgtaat tatgtttgcg ctgcaccagt
     1741 tcttcgggtg agcgggtcat attatccctg aggtagtcaa acccaaactc attattggtc
     1801 ccgtaggtga tatcggctaa gtatgctttt ttgcgtgatt cggagttggg ttcgtgttta
     1861 tcgatacaat ccaccgtaag tccgtgaaac aaaaataaca ttcccatcca ttcggagtca
     1921 cggcgtgcca ggtagtcgtt cacggtaacg atatgcaccc cttttcccgg taaggcatta
     1981 agatataccg gcaaggttgc caccagtgtt tttccttcgc cggtagccat ttcggaaatt
     2041 tttccctgat gcagcaccat gccgccgata agctgcacat cataatgaat catatcccac
     2101 tttattttgg tacctcccgc catccacgcc gaatcataat aagccttatc cccaacaata
     2161 ttgatgcttt cgaatctggc ggccagatca ccatccatag ccgtggcagt aacttccact
     2221 ttatcgtggt cggcaaacct tttggctgtt tctttcacca cggcaaaagc ttccggaaga
     2281 atatcattca gtatttcctg ggttttctca taaacctttt tttccagccg gtcgatttcc
     2341 agataaatct cttctttatc atcaatagat accgattcat cattttcagc tttttctttc
     2401 agcatagcca tctgctcttc gtcttcgtgc acatattcat aaatgcgttc acgaaactcc
     2461 atggttttgg cccggaggcc atcatttgac aattgagaaa ttgccggata aacttctaaa
     2521 cacttttgca atataggttg taagctctta atatcacgat cggattttgt gcctaagaat
     2581 agttttaaaa aatttgccat acatcaataa cattagagaa taattcgcaa aaatacacaa
     2641 aatgccggat gtgtattttg aaaattgaaa ataatatatg atttcatttt gctgttatca
     2701 ttattattta tacttttgca ctcccaaatt ggggaaacta taaattatta attattaaac
     2761 aactaaggaa atgacaaaac attatgaaac cgttttcatt ttaactcccg ttttatctga
     2821 agatcaggta aaggaagcgg taaaaaagta tcatgatttc ctttcagaaa aaaaagctga
     2881 aatcgtggta tcggaaagtt ggggattgcg caaactggct taccccatcc agaaaaaatc
     2941 ttcagggttt taccaactca tcgagtttaa ggctgaacca aacgtaattg ctgacctgga
     3001 agttgcctat aaacgcgacg aaaggctcat acgctttctt accatctccc tcgacaaaca
     3061 tgctgttgct tacagagaaa gaaaacgtgc tgaaaagcaa aataccgaaa aagcttaatc
     3121 aaattgtaaa acctaaaaca ttaaaaacat ggcacaaacc gacagcgaaa tcagatattt
     3181 aactccgatt aacgtagatg ttaaaaagaa aaaatattgc cgcttcaaaa aacacggcat
     3241 taaattcatc gattacaaag acgcagattt cttattgaaa ttcatcaacg agcaaggtaa
     3301 aatattaccc cgccgactga ccggaacttc cttaaaatat cagcgcaaac tggctaaagc
     3361 aataaaaaga gcccgtcatc tggcgttatt accctatgtg gcagatttat taaaataagg
     3421 aggaacaact atgaaagtta ttttaaaaca agatgtaacc aacttgggtt acgccaacga
     3481 aatagtgaaa gtaaaaaacg gttatgcccg taactacttg atccctaaag gtattgccat
     3541 catgctgacg ccttcttctg aaaaagaaca tgctgaactg atgaaacaaa gaacttttaa
     3601 agaagaaaaa atacgcaaag aatccgaaac cctggccaaa gctctcgaaa acatagtggt
     3661 aaaggtgggc gctaaagccg gaacatccgg aaaaattttc ggctcggtta atgctatcca
     3721 gcttgctgat gccattaaac aacaattcaa ttatgaaatt gaccggaaaa aaattgtggt
     3781 ggacggagaa agcgttaaag aattaggcac ctattctgca aaaatcaaca tttataaaga
     3841 catcaaggtt gacatcaaat ttgaagtgat cgcagaataa agatttttct ttataattgg
     3901 ttgttatctg aaaattcccg gctttttggc cgggaatttt tttatttttg taacgatatg
     3961 ctgacaaaaa acaccatcaa atatatcaaa tcattatccg aaaaaaaatt tcggaacgcg
     4021 tatggtagtt ttattgccga agg
//
LOCUS       DAOSFP010000213         4043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_20176_length_4043_cov_3.374122, whole genome shotgun sequence.
ACCESSION   DAOSFP010000213 DAOSFP010000000
VERSION     DAOSFP010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20176_length_4043_cov_3.374122"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..187)
                     /locus_tag="PLI16_06975"
     CDS             complement(11..187)
                     /locus_tag="PLI16_06975"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOH84341.1"
                     /translation="MPQIEIHRNGPAIIKGKCLLKGENGQLSETKDVFALCRCGKSKK
                     TPYCDGSHLLLPFE"
     gene            complement(230..460)
                     /locus_tag="PLI16_06980"
                     /pseudo
     CDS             complement(230..460)
                     /locus_tag="PLI16_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            complement(686..1300)
                     /locus_tag="PLI16_06985"
                     /pseudo
     CDS             complement(686..1300)
                     /locus_tag="PLI16_06985"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            1505..1960
                     /locus_tag="PLI16_06990"
     CDS             1505..1960
                     /locus_tag="PLI16_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84342.1"
                     /translation="MIKKLSGLIFTVFLIGTLHAQQVYKADPRLTECFDQAYLSELEN
                     SQSELLLYYNYYLDNSYYKVDLNTLDKKISAQDIHTVLLQNNNPQVKEYFKETTFTKE
                     KFNPLKYQFNLSSNSFTTYLWEKEGIAIVFYPLSHISANFKEYLKTINK"
     assembly_gap    2113..2212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2213..3958
                     /locus_tag="PLI16_06995"
     CDS             <2213..3958
                     /locus_tag="PLI16_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH84343.1"
                     /translation="KFDSTLFIPDGPACPTQCYNTFVTFMAFIPGATITSANDILSIC
                     VNMEHSYTGDLGFRIICPNGQSVQLDPNTHSGGAYMGAPYGGSSHGTYDNGCDPTNNP
                     YGSGWTYCWSQVYPQHGTLDQLSSGGVSPIDSTNTINHTNYITPNSSLAGLIGCPLNG
                     TWNIEICDDFGIDNGYIFWWELNLDPSLLPTGWGYQVPIDTVTWQGSFFDVINDTTIR
                     VIPDSGGVYTFTVTVTDIFGCTYDTTLQIQVVQTPDVNLGSDTILCGNNLNFVLDAGP
                     GDEFTWSTGGNSQTQPVSSTGYYVVTVENHNITNTLTCSDNDTIFVKVLAQPTMVDLG
                     PDTCSTVPFDLDAQNGGGVFQYDWSTGATSQTINVTSSGTYSVTVAEEFGYNCETTDS
                     RVVTIIPEPEPTIGPDSTICSFNYFRMKVKDPANLLDQHQYSYLWTTVPESNLNGQTA
                     RDVEFGCLTPGVTYNVQVAVTGCNTVTASRNIISQNCQLELYNIITPNGDGSNDKFVV
                     KGLENFPGSNMKVYNRWGKKIFESDTYGEDDDNSWGGEKDADGVYYYVLTVNYGDSEG
                     CVEEINYHGTVTVIR"
BASE COUNT         1091 a          850 c          797 g         1205 t
ORIGIN      
        1 aatgtgttgt ttattcaaaa ggaagcaaca ggtgtgatcc atcgcaatac ggtgtttttt
       61 ttgatttgcc gcaacggcaa agggcaaaaa catcttttgt ttccgataat tggccgtttt
      121 cccctttcag caggcacttt cccttgataa tggccggacc gtttctgtga atttcaattt
      181 gtggcaaagt ggtggcggtg atgctttcct gcagggcttt gtccttctgc ctgtcgttat
      241 aataataggt aagcgcatcg gtagggcaac gtgttatttg tgcaataatt ttttcagtcg
      301 aagccccgta cgggtttatc catggtcttt ccgccgggtt aaaaacttca ggcaattcgc
      361 tgaagcaata ggccacatgg gtgcatagat gcggttgcca cacgatggta atttccccgt
      421 tggtgtattt cctgattacc gtggtgtttt cttcttcaat ggcccttgtg aaattcacgt
      481 aatcattgct gagttgccat aattttaagg cagtattcat gtctttggca tttgccgagg
      541 gctcgacagg ctttttcttt ttaaaatacc ggccgcttac ggtttcaagg tcaggagaac
      601 ttgccagaaa aactgaggtt tcggctcctt tttcaggtga aatcattatt aatttaaaaa
      661 gaaaaatcgc caggggattc atttttttaa agatgctggt ctttacaatg cccgggtgca
      721 ggcagttggc tgtaacacct gtacctttca gctcttttgc cagttgtctg gtaaacaaaa
      781 tgttggccag tttcgattgg tcgtacgctt ttttgccact gtaacctttg ctgaacgaaa
      841 tatcgtcaaa atttatacgt gcccccctgt gagaatccga agccacattg ataatccggg
      901 aaggagcact gttttttaat acctccaata gcaggttggt caacaaaaac ggagccagat
      961 gatttacggc ccagttcatt tcgtaaccgt ctttactttt taagttatca atttcataaa
     1021 tacccatatt gttaataagc acatgcagtt tgtcgtgttt ttgtctaaaa tcatccacaa
     1081 aatttcttat ggatgccaga gaagaaaaat cacagtaaaa acacccgatc tcctgaccgg
     1141 ttttttgcag aattaagtcc cgggtagctt cggctttagc tttgttccgg tatgtgatta
     1201 ttacgcgggc tttcatttgt gccagcgtca tagccgtttg cagtccgatg cccgaggtgg
     1261 ctccggtgat caggcatgtt ttttctttta ttgtccacat ttttttgata ccaaattttc
     1321 aacaaagata aaaataattt agaataattc caaattatat cccggaatgg tgttttttta
     1381 taaataaggc ttaaacaatc tatttttatt gatatttttg taaaaataaa ctacaggcaa
     1441 ccatttttaa aatgttgttg tctataaagc aataaaataa aaaaaacatt aaaccagatt
     1501 taatatgata aaaaagttat cagggttaat ttttaccgtt tttcttatcg gaaccctcca
     1561 tgcacagcag gtgtataagg ccgacccccg gcttacggaa tgctttgatc aggcatatct
     1621 cagcgaattg gaaaattcac agtcggaact tttactgtac tataattatt atttagataa
     1681 ttcgtactat aaagttgacc tgaacacgct ggataaaaaa atttcagcac aggatatcca
     1741 cacagtattg ctgcaaaaca acaatcctca ggtaaaagaa tattttaaag aaacaacttt
     1801 tacaaaagaa aaattcaatc cgctgaaata tcagtttaat ctttcatcca atagctttac
     1861 cacctatctt tgggaaaaag aaggtattgc cattgtcttt tatccgcttt cgcacatttc
     1921 ggctaatttt aaggaatatc taaaaactat taataaatag aactgttaac agaaaatatt
     1981 tgattatgaa aaaaatatta ttactttttg ctctgttatt tgtttttacc gttgcccgtt
     2041 cccaatctta tcttatcagt acgggcggca ccgtaacgtc ctgcggcgaa tatttttatg
     2101 attcgggtgg cannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaatttga
     2221 cagcaccctg tttattcccg atggccctgc ctgtcccaca cagtgctata acacctttgt
     2281 aacttttatg gcgtttattc ccggagcgac cattaccagt gcaaacgaca tactttccat
     2341 ctgtgtcaat atggaacatt cctataccgg tgacctgggt tttcgaatca tctgtcctaa
     2401 cggtcaatcg gtacagctcg accccaatac gcactcgggc ggcgcttata tgggagcacc
     2461 ttatggcggt tccagccatg gcacttacga caacggttgc gatcccacta acaaccctta
     2521 tggctctggc tggacttatt gctggtcgca ggtttatccc cagcacggca cgctcgacca
     2581 gctttcttcc ggcggcgtta gccctattga ttcgacaaat accatcaacc atacaaatta
     2641 tatcacacca aacagctcgc tggccggact cattggttgt ccgctcaacg gaacgtggaa
     2701 tattgagatt tgtgatgact ttggtatcga caatggttac atattctggt gggaactaaa
     2761 cctggatcct tccttgttgc ctaccggatg gggttatcag gtgcctatcg ataccgtaac
     2821 atggcaaggt tcctttttcg atgttatcaa cgataccacc atcagggtta ttcccgattc
     2881 gggaggagtg tacactttta cagttacagt taccgatatt tttggatgta cctacgatac
     2941 tactttacag attcaagtgg ttcaaacacc tgatgtgaat ttgggatccg acactattct
     3001 gtgcggcaat aacctgaatt ttgtgttaga tgctggtccc ggcgatgaat ttacatggtc
     3061 aacaggaggc aactcccaaa cccaacccgt gtcatctacc ggatattatg ttgttacagt
     3121 tgaaaatcat aatataacta acacactcac gtgttcggac aacgatacta tttttgtaaa
     3181 agtgctcgca cagcccacca tggtggatct cggccccgat acgtgcagca ccgtgccttt
     3241 tgacctggat gcacaaaatg gcggaggcgt ttttcagtac gactggtcaa caggtgcaac
     3301 ctcccaaacc attaatgtta cttccagcgg aacgtattcc gttactgtgg ctgaagaatt
     3361 cggctataac tgcgaaacaa ccgattcaag ggtggtaacc atcatccccg aacccgaacc
     3421 tactatcgga ccggattcaa ccatctgtag ctttaactat ttccgtatga aagttaaaga
     3481 tcccgccaac ctgctggatc aacatcagta ttcctatttg tggaccacgg ttcccgagtc
     3541 gaatctcaac ggacagacag cacgcgacgt ggaatttggc tgcctgacac ccggagttac
     3601 ctacaatgtt caggtagccg ttaccggttg taataccgtt accgccagcc gcaatattat
     3661 ctcgcaaaac tgtcagttgg aactgtacaa tattattaca cccaacggtg atggcagcaa
     3721 cgacaagttt gtggttaaag gacttgaaaa cttccctggc agcaatatga aagtatataa
     3781 ccgctggggc aaaaaaatat ttgaatccga tacttatggc gaagatgatg acaattcctg
     3841 gggcggtgaa aaagatgccg acggcgttta ttattatgtt ctaactgtaa attatggtga
     3901 ttctgaaggc tgtgtcgaag aaatcaatta tcacggcacg gtaaccgtta tcagataata
     3961 tttatttttt gaaaaaaaac catcagtcag ccgactgatg gttttttttt ataatttagt
     4021 ccatatttta attcgatgaa aaa
//
LOCUS       DAOSFP010000214         3976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_20693_length_3976_cov_3.709258, whole genome shotgun sequence.
ACCESSION   DAOSFP010000214 DAOSFP010000000
VERSION     DAOSFP010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20693_length_3976_cov_3.709258"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="PLI16_07000"
     CDS             complement(<1..719)
                     /locus_tag="PLI16_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOH84344.1"
                     /translation="MQFKSDIEALEALVEKYNKVKAETAKVIIGQDEVVRNIIISIFS
                     NGHALLIGVPGLAKTLMINTIAKSLGLNYSRIQFTPDLMPSDIIGSEILDEERKFRFI
                     KGPVFANIILADEINRTPPKTQSALLEAMQEKAVTVAGKTFKLDEPFFVLATQNPIEQ
                     EGTYPLPEAQLDRFMFNIWLDYPDFEQEVNIVKTTTTNYMPRVEVVLTAEEILEFQSV
                     IRKIPVPDNVYNYAINLASVTR"
     gene            complement(732..2099)
                     /locus_tag="PLI16_07005"
     CDS             complement(732..2099)
                     /locus_tag="PLI16_07005"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH84345.1"
                     /translation="MTKIINKTVLLFLLLTSSVACIAQQNNEKILIDEVVAVVGSNIV
                     LYSDIENEYFQYLMQGSTKGPGLKCQILEEVMFQKLLLTQAEIDSVTVTDKQVESELD
                     RRMRYFIDQIGSKEKLEEYFKKTVLQIKEEMRSKIRDYLLIQNVQAKLTQNVKITPTE
                     VTEFFSAISPDSLPLVGSQIEVAQIVKKAVISQDEVNMVKEKLNGLRERVLKGEDFAT
                     LANLYSEDPGSMTKGGELGFVGRGELFTEFEAAAFSLKPGEVSPIVKTQKGYHIIQMI
                     ERRGEYINVRHILMIPKASLTDMIKSKKEIDRIAEIIKKDSLSFERAAALYSDDPTKV
                     NGGLLTNQYSGDSKFSPEEIDPNMFFVIEKMKVGEISAPMVYTNEDGQQAYRMLKLKS
                     RTEPHKANLKDDYPQIQNIAVQVKQNQAISDWINEKTQNTFISISDNYKDCNYKYTWL
                     RNDKQ"
     gene            complement(2156..2995)
                     /locus_tag="PLI16_07010"
     CDS             complement(2156..2995)
                     /locus_tag="PLI16_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84346.1"
                     /translation="MKKVFFILAVFLCACHARHAGDEQPLARVYDKYLYASDLQGILP
                     PDMAGQDSMEFVSEYVDNWINQQLIIAQAEKNLSDEEKDFSKQLEEYKNSLTVFQYES
                     KLINQRLDTVVSDEEIARYYELHKENFVLKENIVKVVYVKIPKEVSPAPVRNLIKSGG
                     QQDMKKLQDFCHKYAVNYYLDDQSWLLFNDILKEIPINTYNQEEYLKNNRIIEIQDSA
                     FTYLMNIKGFMIKEGVSPLSFEQSNIRDMIINKRKLTLIENMRKDIYDNALQKKDFEV
                     FKN"
     gene            complement(3051..>3976)
                     /locus_tag="PLI16_07015"
     CDS             complement(3051..>3976)
                     /locus_tag="PLI16_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84347.1"
                     /translation="KARIIKDSRANKSKESVINRIMGEYGFKELPGVVNDFYAVVDST
                     IYEGKWEPAKAASLTKPMFTIGEKTITQQDFAKFLGTKQGRRIKMKITDFVNSRYDDF
                     IDESCLQYEDSKLEQKYPEFKALMKEYRDGIMLFELTDKKVWSKAIQDTTGLTAFHEN
                     NKSKYMWDERLDASIFTCNNQATAKSARKLVKNYINGKMTEMDIYKKINIDTVPTLKI
                     EHKKYSRKDNPVIDQIKWEKGVTEDISKDDKVIFVVVHNLVAPEPKLLKEAKGLITAD
                     YQNYLEEEWIKQLRSKYPYVVNQGVFDSLLK"
BASE COUNT         1011 a          841 c          806 g         1318 t
ORIGIN      
        1 cgggttaccg aagccaggtt tatggcataa ttatacacat tgtcgggcac gggaatcttg
       61 cggatgaccg attgaaattc aagaatttcc tcggccgtga gaaccacttc tacacggggc
      121 atgtaattgg tggtcgtagt ttttacaata ttgacttcct gttcaaaatc ggggtaatca
      181 agccaaatgt taaacataaa ccggtcgagt tgcgcttcag gcaaaggata agtgccttcc
      241 tgttcgatag ggttttgcgt ggctaataca aaaaacggct catcgagttt aaaagttttt
      301 ccggccacag taacggcctt ttcctgcata gcttcgagca gggctgattg tgtttttgga
      361 ggggtgcggt taatctcgtc ggcaagaatt atgttggcaa agacaggtcc tttgataaac
      421 ctgaatttgc gttcttcatc aagaatttcg gagccaatga tatcggatgg catcaggtcg
      481 ggggtgaact gtatcctgga atagttcagc cccagggatt ttgcaatagt attgatcatc
      541 agcgttttgg caagaccggg gacgcctatg agcagggcat gtccgttgct gaaaattgaa
      601 attatgatgt tgcgtaccac ctcatcctgt ccaataatca ccttggccgt ttcggctttc
      661 accttgttat acttttctac cagcgcttcc agagcttcta tatcggattt gaattgcatc
      721 gttacagttt tttattgttt atcgttacgg agccaggtat atttataatt gcagtcttta
      781 taattatcgg aaatactgat aaaagtgttt tgtgtttttt cattgatcca atcgctgata
      841 gcttgatttt gcttaacctg aacagcaata ttttgtattt gagggtagtc atcttttaaa
      901 ttggccttat gcggctcagt gcgtgattta agtttcagca tacggtaggc ctgttgccca
      961 tcctcattgg tataaaccat aggagccgaa atctctccga ctttcatttt ttcgatgaca
     1021 aaaaacatat tggggtcaat ctcttcgggc gaaaatttcg aatcacccga atactggttg
     1081 gtaagcaatc cgccgttaac tttggtgggg tcgtcagaat acagtgccgc agctctttca
     1141 aaagataggg aatctttttt aataatctcg gcgatgcggt caatttcttt tttcgatttt
     1201 atcatatctg tcagcgaggc tttaggaatc ataagtatat gcctcacatt aatatattca
     1261 ccccggcgtt caatcatttg aatgatatga tatccttttt gagttttcac gatgggggaa
     1321 acttctccgg gtttcagcga aaaagctgcc gcttcaaatt cggtgaaaag ttctcctctg
     1381 cccacaaagc cgagctcacc acccttggtc atagagccgg gatcttcgga atacaaattg
     1441 gccagggtgg caaaatcttc gccttttaac acacgctcac gcaggccgtt gagcttctct
     1501 ttaaccatgt ttacctcgtc ctggctgatg acggcttttt tcacaatctg agccacttct
     1561 atctgcgaac ccaccagcgg caggctgtcc ggagaaatgg cactaaaaaa ctcagtaact
     1621 tccgtgggag taattttcac attctgcgta agcttggcct gcacattttg tattagcagg
     1681 taatcgcgta ttttgctgcg catctcctcc ttgatctgaa gcacggtctt tttgaaatat
     1741 tcctccagtt tttcttttga accaatctgg tcgataaaat accgcatcct gcggtccagt
     1801 tcgctttcca cctgtttatc ggtaacagtc acgctgtcaa tttcggcctg ggtaagcaac
     1861 aatttctgaa acatcacctc ctccagaatc tggcatttga ggccggggcc tttggtgctg
     1921 ccctgcatca ggtattgaaa atattcgttt tcgatatcgg aatacagcac gatgttgctg
     1981 cctaccaccg ctaccacctc gtcgataagg attttttcgt tattttgctg tgctatgcac
     2041 gcaactgatg aggtgagcaa cagaaataac agaacggttt tatttataat tttcgtcatt
     2101 aattcggggt gttatctgat aataactatg taaactctaa attagtatga atgtattaat
     2161 ttttaaaaac ttcaaaatct tttttctgta aggcattatc ataaatatcc ttcctcatat
     2221 tttcaatcag cgtaagcttc cttttgttga taatcatatc gcggatgttg ctttgttcaa
     2281 aactcagcgg cgatacgcct tctttgatca taaatccttt gatattcatc agataagtaa
     2341 aggccgaatc ctgaatctca ataatgcggt tgtttttcag atattcttcc tgattatagg
     2401 tgttgatggg gatttcttta aggatgtcgt tgaacaacag ccagctctga tcatctaagt
     2461 aataatttac agcgtattta tggcaaaagt cctgcagttt tttcatgtcc tgctgaccgc
     2521 ccgacttaat aaggttgcgt acaggggccg gcgatacttc cttgggtatt ttgacataga
     2581 caaccttaac aatgttctcc ttcaatacaa aattttcttt gtgcagctca tagtagcggg
     2641 caatctcttc gtcagaaacc accgtgtcaa gcctttggtt gataagtttt gactcgtatt
     2701 gaaaaaccgt gagggaattt ttgtattctt cgagttgttt cgaaaaatct ttttcttcat
     2761 ccgaaagatt tttctcggcc tgggcaataa tcaattgctg atttatccag ttatcaacat
     2821 attcgctgac aaactccatg ctgtcctgac cggccatatc cggcggaagt attccctgca
     2881 gatccgaagc atagaggtac ttgtcataca cacgggccag gggctgttcg tctccggcat
     2941 gcctggcatg acatgcacac aaaaaaacag caagaataaa aaagactttt ttcaaaaaat
     3001 attttatcaa aataccgaaa gaaacggtta cgtattaatg attataagcc ttatttcaac
     3061 aacgaatcaa aaacgccctg gttgactaca tagggatatt tgcttctgag ttgttttatc
     3121 cattcctctt caagatagtt ctgatagtcg gcagtgataa gtcccttggc ctctttcagc
     3181 agtttgggtt cgggcgccac caggttatgc accaccacaa aaataacttt atcatcttta
     3241 ctgatatctt cagttacgcc tttttcccat ttgatttgat caataacagg gttatctttt
     3301 ctcgaatact ttttatgttc aattttcagt gtgggtaccg tatcaatgtt gattttttta
     3361 taaatgtcca tttcggtcat ttttccgttg atatagtttt tcactaattt gcgggcactt
     3421 ttggctgttg cctgattatt gcaggtaaat atcgatgcat caagacgttc gtcccacata
     3481 tatttgcttt tattattttc gtggaacgca gtcaggcctg tagtatcctg tatagctttt
     3541 gaccagactt ttttgtcggt aagttcaaaa agcatgatac catcgcggta ttctttcatc
     3601 agcgccttaa attcaggata tttttgctcg agttttgaat cttcgtactg caaacaactt
     3661 tcatcaataa aatcatcata acggctatta acaaaatcag tgattttcat cttgattctc
     3721 ctaccctgct ttgtgccgag aaatttggca aaatcctgtt gtgttatggt tttttctccg
     3781 attgtaaaca tgggtttggt aaggctggct gcttttgcag gttcccattt gccttcataa
     3841 atggtagaat ccaccacagc gtaaaagtcg ttgacaacac ccggcagttc tttaaatcca
     3901 tattcgccca tgatgcgatt gatcactgat tctttgcttt tgttggcacg gctgtctttt
     3961 attatccggg ctttaa
//
LOCUS       DAOSFP010000215         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_20777_length_3967_cov_4.338190, whole genome shotgun sequence.
ACCESSION   DAOSFP010000215 DAOSFP010000000
VERSION     DAOSFP010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20777_length_3967_cov_4.338190"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PLI16_07020"
     CDS             complement(<1..965)
                     /locus_tag="PLI16_07020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOH84348.1"
                     /translation="MKNEKEFDFYKLDNGLRIVHKNSDTPIVHLGLFINAGSRDEIPA
                     QKGLAHFIEHVFFKGTETRRSSQIYNSIENYGGELNAYTAKEETCIHCSVLKEYFEKA
                     AEVLSDVLFHSTFPEKELAKEKAIVLDEYYYYKDIPEEVILDDFDELVFKNHPLGWNI
                     LGEPVRIKGFTKNDIKTFMDNNYHPEEMVISLIGGLDSQTILRVLKKWFEQGYSSGRT
                     ATKRKAFKGYKPVRQHLVKNIFQSHCVMGRPAYTVYDKRRLGLVLLNNLLGGPASNAR
                     LNMAVREKHGLSYNVESSYSAFSDTGLFRIYVSTENGSMDKTIAL"
     gene            1090..1680
                     /locus_tag="PLI16_07025"
     CDS             1090..1680
                     /locus_tag="PLI16_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOH84349.1"
                     /translation="MKNIAVFCGSSAGGRPIYGEKARQLGKLFVKHGFSLVYGSGNVG
                     LMGIMADSMLEEDGEVIGVIPQRLVDVEVAHKHISRTHIVATMSERKMLIEQLSDAFI
                     ILPGGMGTLDEFFEMLTLGQLGIIKKPIGVFNIEGYFDKMIAMLDYFIEERFMRPEHK
                     ELFFVSDDEKELIERLKTFQPKETVKWLENFKHTKF"
     gene            1682..2161
                     /locus_tag="PLI16_07030"
     CDS             1682..2161
                     /locus_tag="PLI16_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOH84350.1"
                     /translation="MIKTGAKILDDLSTAAAGSARQRKNHNFHRGEHEVLQRMLNALC
                     PGTYIRPHKHADPGKNEVFIILRGKALVVEFDKDGNISDNFILDKTCENYAAEIPPEH
                     YHTIIALDAGTVVYEIQEGPYSPVTDKIFAPWSPEEGSQEATSFLHRIFDEVGISCQ"
     gene            2184..2915
                     /locus_tag="PLI16_07035"
     CDS             2184..2915
                     /locus_tag="PLI16_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOH84351.1"
                     /translation="MKKTLVVAHRGGSLLAPENTLAAFRQALALGVDRIELDVQQSKD
                     GVVLVIHDEKTNRTTNGKGTISDMNYAQLKDLDAGSKFSKEFSNEKIPLLDEVLCLIN
                     GQCELLVEIKNPGNINIGLEKNVADLIQQHRALAWCTVQSFEYESLYRLHKINDQITL
                     GLLLDKPKPDTLKKSAANTSFISEININRRSASAAMIDLIHQMEKKVYVWTVNKPGDM
                     VRLIKNGVDGIITDDPKTLKALLCG"
     gene            3013..3089
                     /locus_tag="PLI16_07040"
     tRNA            3013..3089
                     /locus_tag="PLI16_07040"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3047..3049,aa:His,seq:gtg)
     gene            3154..>3967
                     /locus_tag="PLI16_07045"
     CDS             3154..>3967
                     /locus_tag="PLI16_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOH84352.1"
                     /translation="MADKRGIIPRKIKGFRDIDPNLNQLKWKIIHAASSIYRLYGFEH
                     WDTPILEYADNLGKYLPDTDTIEEGVYSFRNPEKEPVLKTDGKELRDEWDKVIMENHF
                     LSLRYDLTAPLARLYAEKLWLDSQKGNVNSANAPLFRRYQYGPVYRYETKLDPGRFRE
                     FWQLDFDSVGTSNVASDAEVCMILSDAMEAIGLKRNTYLVKVNNRKVLKGFLQSIGIH
                     TEAEEQSILRIIDKTDKIGIKGLTEELGAGRKDVSGAFIAGLKLPNDKIDAIL"
BASE COUNT         1169 a          876 c          921 g         1001 t
ORIGIN      
        1 atcagcgcga tggttttgtc catgctgcca ttttcggtgc tcacatagat gcgaaacaat
       61 ccggtgtcgg aaaacgccga atagctggat tcgacattat aggacaggcc gtgcttttca
      121 cgtacagcca tgttcagccg ggcattggaa gccgggccgc ccagcaggtt attaagcaac
      181 accagtccga gccggcgttt gtcgtacacc gtataggccg gccttcccat aacacaatgc
      241 gactgaaaaa tatttttcac caggtgctgc ctgaccggct tgtatccctt gaacgctttc
      301 cttttggtgg ccgttctacc ggatgaatac ccctgttcaa accatttttt taataccctg
      361 aggattgttt gggagtcaag accgccgata aggcttatga ccatctcttc cggatgataa
      421 ttattgtcca taaaggtttt tatatcattt ttggtaaacc ctttaatcct gaccggttcg
      481 ccaagaatgt tccagcccag gggatgattt ttaaaaacca attcgtcgaa atcatcaagt
      541 atcacttctt cggggatatc tttataataa taatattcgt ccagaacgat ggctttttct
      601 ttcgccagtt ctttttcagg aaaagtggaa tggaacaaca cgtcggaaag cacctcggcg
      661 gctttttcaa agtactcttt cagcacggaa caatggatgc aggtttcttc cttggccgta
      721 taggcattga gttcgccgcc gtaattttca atgctgttgt aaatctggct ggaacggcgc
      781 gtttcggtcc ccttaaaaaa aacatgctca ataaaatgtg ccagtccttt ttgtgccggt
      841 atctcgtcac gtgagcccgc attgataaaa agcccaagat gcacgatggg cgtatcgctg
      901 tttttgtgaa caatgcgtaa cccgttgtcc agcttataaa aatcaaactc tttttcgttt
      961 ttcatacacg caaaggtagg acaaattcag aagtcagaag ccggaaataa gaattctggc
     1021 ttccggctta agaaattgaa ttttgagttg ttcataataa gtttaatttt gtgatataaa
     1081 atctttatta tgaaaaacat cgctgttttt tgcggttcat cggcaggagg acggccaatt
     1141 tatggagaaa aagcaaggca attaggaaaa ctatttgtga aacatggttt ttcgctggtg
     1201 tatggttcgg gaaatgtagg tttgatgggc attatggccg actccatgct tgaagaagac
     1261 ggcgaagtga tcggtgtcat tccgcagcgt ctggttgatg tggaagtagc ccacaagcac
     1321 atctcacgca cgcatatcgt ggccaccatg agcgaacgca agatgcttat cgaacagctt
     1381 tccgatgcgt ttatcatcct gcccggaggc atgggcaccc tggatgagtt ttttgaaatg
     1441 ctgaccctgg ggcaattagg aattataaaa aagcccatag gcgttttcaa tattgaggga
     1501 tacttcgaca aaatgattgc catgctggat tattttattg aggaacgctt tatgagaccg
     1561 gaacacaaag aattgttttt tgtcagtgat gacgaaaaag aactgattga acgcctgaaa
     1621 acctttcagc caaaagaaac ggtaaagtgg cttgaaaatt ttaaacacac aaaattttga
     1681 aatgatcaag acaggcgcaa aaatccttga cgatctttcg accgctgcgg ccggctcggc
     1741 tcgtcaaagg aaaaaccaca actttcaccg gggtgagcac gaagtgcttc agcgtatgct
     1801 caacgccctg tgtccgggca catacatccg tccgcacaaa catgcggatc ccggaaaaaa
     1861 cgaagtcttc atcatcctgc gcgggaaagc gcttgtggtt gaatttgata aggacggaaa
     1921 catcagcgac aattttattc tggacaaaac gtgtgaaaac tatgcggccg aaatacctcc
     1981 ggagcattat cacaccataa tcgcgctgga tgccggcacg gtggtttacg aaatacaaga
     2041 aggtccctat tcgcccgtga ccgataaaat cttcgcaccc tggtcacccg aagaaggcag
     2101 tcaggaggca acatcatttc tgcatagaat ttttgacgaa gtgggtataa gttgtcaata
     2161 atttgccatc aaaaacaaat gctatgaaaa aaaccctggt agtagcgcat cgcggaggaa
     2221 gccttttggc tcccgaaaac accttggccg ctttccggca ggccctggcg ctgggcgttg
     2281 accgcataga acttgacgta cagcaaagca aagatggcgt ggtgctggtg atacacgacg
     2341 aaaaaacaaa ccgtaccaca aacgggaaag gcactatcag cgacatgaac tatgcacaac
     2401 tgaaagatct ggacgccgga agcaaatttt caaaagagtt cagcaatgaa aagatccctt
     2461 tgctggatga ggttttgtgc ctgatcaacg ggcaatgtga gctgctggtg gaaattaaaa
     2521 atcccggaaa catcaatatt ggcctggaaa aaaatgtggc tgacctcata cagcagcacc
     2581 gggctttggc atggtgcacc gtgcaatctt ttgaatacga atcgttgtac cgcctgcata
     2641 aaatcaacga ccaaatcacc ctgggactgc tgcttgacaa acctaagccg gacactttaa
     2701 aaaaatccgc agccaatacc agcttcatca gtgaaataaa tataaaccgc cgcagtgcca
     2761 gcgccgccat gattgatctg atacaccaga tggaaaagaa agtttatgtg tggacagtaa
     2821 ataagcccgg ggatatggtc cggctgataa aaaacggggt tgacggcatc attaccgatg
     2881 acccgaaaac attaaaagcc ttgctgtgtg ggtaatcctg atgacttttc cactttggtg
     2941 aaaaaaaata ttccaaaata aaacagcaac gatttcattt ttataaaaag ttgtatcttt
     3001 gcattccaaa atgccacctt agctcagctg gttagagtgt cagattgtgg atctgaaggt
     3061 catgggttcg aatctcatag gtggtaccaa aaaaagcacc gcaccttttg gtgcggtgct
     3121 ttttgttttt atcttcgtaa aaaatttttt accatggctg acaaaagagg cataataccc
     3181 agaaaaataa aaggattcag agatattgat cccaacctta atcagttgaa atggaaaatc
     3241 attcacgccg ccagcagcat atacaggctt tatggttttg aacactggga cacgcccata
     3301 cttgaatatg ctgataattt aggcaagtac cttcccgaca ccgataccat cgaagaagga
     3361 gtgtattctt tcagaaatcc cgaaaaagaa ccggtgctaa aaaccgacgg caaagaactc
     3421 agggatgaat gggacaaggt tatcatggaa aaccatttct tatcgctgag gtatgatctg
     3481 acagcccctc tggcaaggct atacgccgaa aaactctggc tcgacagcca gaaaggaaat
     3541 gtcaactcgg caaatgctcc tttgttccga agatatcagt atggaccggt gtaccgctac
     3601 gaaaccaagc tcgatcccgg gaggtttcgt gaattctggc aattggattt cgactcggtg
     3661 ggtacttcca acgtggcctc cgatgccgag gtctgcatga tattgtctga tgccatggaa
     3721 gccatagggc tgaagagaaa cacctatttg gtaaaagtaa acaaccgtaa ggtgctcaag
     3781 ggatttctgc aaagcatagg tatccatacc gaagcagaag aacaatctat tttacgaatc
     3841 attgataaga ccgacaaaat tggcatcaaa ggactgaccg aagagctggg tgccggccgc
     3901 aaagatgtgt cgggggcttt tatcgcaggg ctgaaactcc ccaatgacaa gatagacgcc
     3961 atccttt
//
LOCUS       DAOSFP010000216         3965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_20797_length_3965_cov_4.412020, whole genome shotgun sequence.
ACCESSION   DAOSFP010000216 DAOSFP010000000
VERSION     DAOSFP010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20797_length_3965_cov_4.412020"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="PLI16_07050"
     CDS             <1..1360
                     /locus_tag="PLI16_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH84353.1"
                     /translation="REQNVQKNNTIELLWDKYSAVAMVPAKDIEAVSPLVAANWGQDC
                     YFNAACPSAPGGPCYHVYAGCVADAMAMVMKTHSWPAHGYGSHSYVHSTANGFPNNYG
                     TLSANFGNTTYNWSSMPNNVYSANSALATLMYHCGVSVNMSYDDQGSGALLTDATNAL
                     IKYFIYDAQYALRSSYSDTDWALMIKTDLDAGLPVIYGGTDTQSGGHAFVCDGYQGTG
                     SYSSYFHFNWGWSGSSNGYVYLNNIAPSGTSFDFDDYQNIVYGIAPNTDVVTPVAGFT
                     ANKTSLAINGLVVFTNSSTNDPIDYSWSITPSTGISYMASTSSTAKNPVVKFTVPGYY
                     TISLTSTNSAGSDTETKTNYIYVYSTAGVEEQELSKNIMLFPNPAQDFITIQTDALDV
                     SEISLKVYDILGNTVAADLHCQNAGNNQLVVNLSSLEKGMYFITISTGKHSVTKKVTL
                     TK"
     gene            complement(1369..2160)
                     /locus_tag="PLI16_07055"
     CDS             complement(1369..2160)
                     /locus_tag="PLI16_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOH84354.1"
                     /translation="MNNRVPFLVICCVAMLLLFSCRTKQAVLELKPGKTVDGDSSAAM
                     KIQPFLFHTLSLKFDAEVQSGDENNNFSGNIYVVRDSALWVSVQKFGLEVVRVLITND
                     SVRMLDRINKSYLAGDFSLISGILKTSIDFDGLQSLITGNDVMSYEDAGYTEKNTGDT
                     LVYYFAKRKKKTGTSSISQEIMVNKVDNKICKNTINNLSQNSGWASFSYSDFESFDGQ
                     KYPGKIAFQVSNTGTLEGIIEFTRITPEKKESLPFNVPDTYVRKN"
     gene            complement(2153..3904)
                     /locus_tag="PLI16_07060"
     CDS             complement(2153..3904)
                     /locus_tag="PLI16_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84355.1"
                     /translation="MKISRKIVFFSCVCLSLLMCYACRTNQPASKPVAADNSKKLTQQ
                     QLIQNTALFLEGLREKYLNNNDKAMGLFAQCIKQNPANDGALYEMGFLLFEQNKYDDA
                     LVLIKDACKRKPDNVWYSLLLAQIYSAKKDFNSAEKVYKTLTDKNPDKPELFYEWADV
                     CILHENYTEAIKVYDLLEDHIGRQPETTLQKEKLYMQLGKSDKAIEEAKAIIRLFPGE
                     TQYYEDLADLYMATGQSLRAFEQYQKILTIDASNPQVHLALADYYRSQNNTEKAIDEL
                     KTAFSSRELDIDSKVKILLTLMNNPVKNKEYFDALPELGKIITEASPEEAKAFSVYGD
                     ILNQQKDLAGARNAFRKVTEIDNSKYVVWQQLLQIDRALEDYDALAADSKSAMELFPE
                     QPEPYLFFGLARMKAGDYEEATRILNTGKNFSIGDDKMLLKFYSALADSYYQIKKYDL
                     CFESFEKSLSIDPNNTYVLNNYSYYLALQNTNLARAQQLCERLLSLYPAEPIFQDTYA
                     WVLFKQKKFEEAKSWSTKAVAGSSEKNGGILEHHGDILFMLQDVEQAVSYWKKAKENG
                     IATEMLDKKIAEKKLYE"
BASE COUNT         1062 a          928 c          799 g         1176 t
ORIGIN      
        1 ccgtgaacaa aacgtacaaa aaaacaacac catcgaatta ttgtgggata agtattctgc
       61 agttgccatg gtcccggcaa aagatattga agcagtgtcg cctttggttg cagccaactg
      121 gggtcaggat tgttatttta acgcggcctg cccttcggcg ccgggaggcc catgttatca
      181 tgtatatgcc ggctgtgttg ccgatgcgat ggccatggtt atgaaaacac acagttggcc
      241 tgctcatggc tatggatcac acagctatgt tcattctacc gccaatggtt ttccaaataa
      301 ctacggcaca ttaagcgcta atttcggcaa caccacctat aattggtcat ccatgcccaa
      361 taatgtttat tcggccaatt cggcccttgc caccttgatg taccattgcg gcgtatctgt
      421 caatatgagt tacgacgatc agggttccgg agccctgttg actgatgcca ccaatgcatt
      481 gataaaatac tttatttatg atgcccagta tgccctgcgc agttcttaca gcgataccga
      541 ctgggcctta atgataaaaa ccgacctcga tgccggcctt cctgtaatct atggcggcac
      601 cgacacacaa agcggcggcc atgcttttgt ttgcgatggc taccagggta ccggaagcta
      661 ttccagctat ttccatttca actggggatg gagcggctca tccaatggct atgtgtacct
      721 gaacaatatt gctcccagcg gcaccagttt tgattttgac gactatcaga acatagttta
      781 tggtatcgca cccaataccg atgtggtaac ccctgtagca ggttttaccg ccaacaaaac
      841 cagcttggcg atcaacggat tggtggtttt cactaattca tcaaccaacg accctattga
      901 ctattcatgg tcgataacac catccacagg aatttcctat atggcctcta catcctccac
      961 ggcaaaaaat cccgttgtga agttcactgt tcccggctat tataccattt cgcttacctc
     1021 gacaaactcg gccggtagcg ataccgaaac gaaaaccaat tatatttatg tatattctac
     1081 tgccggtgtt gaggaacagg aactatccaa aaacataatg ctcttcccaa accctgcaca
     1141 agattttatt accattcaaa ccgatgccct tgatgtttca gaaatcagcc tgaaagttta
     1201 tgacatcttg ggtaataccg tggccgccga cctccactgc caaaatgcag gaaacaatca
     1261 gttggtggtt aatctttcgt cactggaaaa aggaatgtat tttatcacca taagcaccgg
     1321 aaaacatagc gttacaaaaa aagtaacact gaccaaataa gggcactatt aattttttct
     1381 gacataggta tccggaacgt tgaagggaag ggattctttt ttttcgggag tgatgcgtgt
     1441 aaattcaata atcccttcaa gggtgccggt gttgcttacc tgaaaggcaa ttttaccggg
     1501 atatttttgc ccgtcaaaac tctcaaaatc cgaataggaa aacgaagccc atcccgaatt
     1561 ttgcgaaagg ttgttaatgg tgtttttgca gattttgtta tccactttat taaccatgat
     1621 ttcttggctt atcgatgaag tcccggtttt cttttttctt tttgcaaaat aatacaccaa
     1681 cgtatcgccg gtattttttt cggtatagcc ggcatcctcg tacgacataa catcattacc
     1741 ggtgatcagc gactgcaggc catcaaaatc aatactggtt tttaaaatac cggaaataag
     1801 ggaaaaatct cctgccagat aacttttgtt aatccggtca agcattcgca ccgaatcatt
     1861 ggttatcaaa acccttacca cctccagccc gaatttctga accgaaaccc aaagggcgct
     1921 gtccctgacc acataaatat ttcctgaaaa attgttattc tcatcaccgg attgcacttc
     1981 cgcatcgaat ttcagcgaca gcgtatgaaa aagaaacggc tggattttca ttgcggcaga
     2041 agaatctcca tcaaccgttt tgccaggctt cagctcaagc accgcctgtt ttgtcctgca
     2101 tgaaaataac aacagcatcg ccacacaaca aattaccagg aaaggaaccc tgttattcat
     2161 agagcttttt ttctgcaatt ttcttatcca gcatttcggt ggcaatacca ttttcttttg
     2221 cttttttcca atacgaaaca gcttgttcaa catcttgcaa cataaacaat atatcgccgt
     2281 gatgttccag tatcccgccg ttcttttcgc tacttcctgc cacggctttt gtgctccagc
     2341 ttttggcttc ctcaaacttt ttttgcttaa aaagcaccca ggcataagta tcctgaaaga
     2401 ttggttcggc aggataaagg ctgagcaggc gttcgcacaa ctgctgggcc cttgcgaggt
     2461 tggtgttttg cagagcaagg tagtaactgt aattgttcag cacgtaagta ttgttggggt
     2521 cgatgctgag tgatttttca aaagattcaa aacacaaatc atactttttt atctggtaat
     2581 aagaatctgc cagggctgaa taaaacttca acaacatttt atcatcacct atggaaaaat
     2641 ttttaccggt attcaatatc ctggtcgctt cttcataatc accagctttc attctggcga
     2701 gaccaaaaaa caaatagggc tctggctgtt caggaaaaag ctccattgcg cttttactgt
     2761 cggcggccag ggcatcataa tcttccaaag ccctgtcgat ttgcagtaat tgttgccaca
     2821 ccacatattt gctattgtca atttcggtaa ccttgcggaa cgcgttgcgg gcacccgcca
     2881 aatctttttg ctgatttaaa atatcgccgt acaccgaaaa cgccttggcc tcctcggggg
     2941 aagcttctgt aatgattttg cccagttcgg gcaaggcatc aaaatattcc ttgtttttca
     3001 caggattatt catcaatgtc agcagtattt taaccttgga gtcgatgtcg agttccctgc
     3061 ttgaaaaggc cgtttttagt tcatcgatag ccttttccgt attgttctgg ctacggtaat
     3121 aatcagcaag agccaggtga acctgaggat tggaggcgtc tatggtgagt attttctggt
     3181 actgttcaaa ggccctaagc gattgtccgg tggccatata caagtccgcc aggtcttcat
     3241 aatactgagt ttctcccgga aacagccgaa taatcgcttt ggcctcctca atagctttgt
     3301 cgcttttgcc cagctgcata tacaattttt ccttttgtag agtggtttcg ggctgccttc
     3361 ctatatggtc ttcaagcaaa tcatagactt ttatggcttc ggtataattt tcatgcagga
     3421 tgcatacatc ggcccattca taaaaaagct caggtttatc ggggtttttg tcagtaagtg
     3481 tcttatatac tttttcagcg ctgttaaaat ctttttttgc ggaataaatc tgtgccagca
     3541 acaaagaata ccaaacatta tcaggttttc tcttacaggc atctttgata agcaccagcg
     3601 catcgtcata tttgttttgc tcaaacaaca aaaagcccat ctcatacaaa gcgccgtcgt
     3661 tggccgggtt ctgtttaata cactgagcaa aaagccccat ggccttatca ttgttgttaa
     3721 ggtatttctc tctcagacct tccagaaaca gagctgtatt ctgaatcaat tgttgttggg
     3781 tgagtttctt gctattgtcg gcagccacag gctttgatgc gggttggttt gtgcggcagg
     3841 cataacacat caaaagcgaa aggcatacgc atgaaaagaa tactattttt cggctaattt
     3901 tcatctgcat attttaacac atagtggtat aatcccctat gctcaattca tacaatttgc
     3961 ctttg
//
LOCUS       DAOSFP010000217         3959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_20845_length_3959_cov_2.751025, whole genome shotgun sequence.
ACCESSION   DAOSFP010000217 DAOSFP010000000
VERSION     DAOSFP010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20845_length_3959_cov_2.751025"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLI16_07065"
     CDS             complement(<1..302)
                     /locus_tag="PLI16_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOH84356.1"
                     /translation="MATEETNKTNEEPKEEPKKEESFWEKLKDKAEDAWEKVEDAAEK
                     IGDKAEDVWEKVEDKAEDVWEKTKDKTGDTIEKTGDKAEDVWDKTKDKATDIWNKG"
     gene            511..1782
                     /locus_tag="PLI16_07070"
     CDS             511..1782
                     /locus_tag="PLI16_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOH84357.1"
                     /translation="MKKLLTLVLTLLAVFLLFSESTAQSSGKLKYVFLFIGDGMGSNH
                     VFLTEKYNELTKSEPLIFMNGHWSFGLVKTDCADSNKITDSGAGGTALACGERTNYGM
                     IGEYQGKPLESIAEYLHKEKKFKVGIITSVPLNHATPASFYVHEPTRRNYDNISHGII
                     ESGFEFFAGGGFQFGNADTTKKIYRDFNAVVHNMTENNYQLVYEEAQLTADNAASFHH
                     VVIIDTAIRNRQTALKNVYPEERNSLPFALDFPEYKMQLAKYTEKAQNFLFNDKGFFI
                     MVEGGKIDWASHDNDALAAIHEVNAFNEAIKKAYEFYLKHPKNTLILVTADHETGGLS
                     LGSGQDEYGAVKSNKYALYIRKLLQQKKSYIFTDKESLKKIQEDAQIGWTTDEHTAAP
                     VGIWAIGRGSENFSGIMKNSDLKTKILHLIK"
     gene            complement(1813..2739)
                     /locus_tag="PLI16_07075"
     CDS             complement(1813..2739)
                     /locus_tag="PLI16_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOH84358.1"
                     /translation="MADVAFIIDGRKKDQDLLFQTYETYFGQENRVKSFITTHAQHGI
                     RLAEVALQQGFTHVISVGGDGCLHEVVNGMLKAKKHLTEEQWLKIRLGVLPKGSGNDF
                     VRTVAVPENAQSLLGAIQNDKTRLIDVCSATYESVAGGEESRYMANVADVGMGAVVVQ
                     KQSAYSKRLSAFFNYQRAIIGTFFTYKKNHIKVTADSFTYEGNVMDFVINNGKYFGNG
                     IGIAPDALPDDGLLSVTLLGDISILDYLRQLPKARKCKKIIHPEIHYHSARELSIESL
                     SGKAPFEIDGEFVGYTPVKVSIEPAALRVLVV"
     gene            2959..>3959
                     /locus_tag="PLI16_07080"
     CDS             2959..>3959
                     /locus_tag="PLI16_07080"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004912.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOH84359.1"
                     /translation="MKHKIALIGFGTVGQGIAEILLKKKEYLKDNFKFDYDLVAVADF
                     FYGNCYNPKGLDTTYLLKTAKAKKKFSKDLSTKDNLSLIKSCNADIVCELTYTDLKTG
                     GPALGHVKAALSSKKHVITSNKGPAALKYQEFSKLAKRNGVKFMIEGTVVSGTPVINL
                     SKGPLAGCEISKIKGILNGTTNYMLTEMEKGMDYASVLKVAQDLGYAEADPTGDVEGY
                     DARGKVCILANVVMGVPLNFNDIACKGITGITLGDIEKAKKEGKRWKLIGCVEKKGNK
                     VVGSVNPEMVELSHPLAGVMGAKNALTFTTDMLGDVTIIGPGAGKTETGFSILTDMLA
                     "
BASE COUNT         1193 a          835 c          813 g         1118 t
ORIGIN      
        1 atgcctttgt tccaaatgtc tgtagcttta tctttagtct tatcccaaac atcctctgcc
       61 ttgtcgccgg ttttttcgat agtatcgcct gttttatctt tggttttttc ccagacatct
      121 tcggctttat cttctacttt ttcccagaca tcctcggctt tgtcgccaat tttttcagcg
      181 gcatcttcaa ccttttccca ggcatcttca gccttgtctt taagtttttc ccagaaactt
      241 tcttcttttt taggctcttc cttgggttct tcgttggttt tgtttgtttc ttctgttgcc
      301 ataattataa tatttatttt tagtgtaaat ataacaaaaa atattgtgaa atatttaatt
      361 gttgaaatat ttttgagtac acaaatccaa aaggcaatgc cgaaaaagtg cccatgcctt
      421 cacatgccgt gtagtatgtg cggaaatatt tgcttaagaa gctatcataa tcaaaaatac
      481 ccgtactttt gacaaaataa accggccttt atgaaaaagt tgctaaccct tgtactgacc
      541 ttacttgctg tattcctcct tttttctgaa tctactgcac aaagctcagg aaaattaaaa
      601 tatgtttttt tgtttattgg cgacggcatg ggctccaatc atgttttcct caccgaaaaa
      661 tataacgagc ttaccaaatc agaaccgctt atttttatga atggtcattg gtcgtttggc
      721 cttgtaaaga ccgattgtgc cgacagcaac aaaattaccg attcaggcgc cggtggcaca
      781 gccctggctt gcggtgagcg gactaattac ggcatgattg gcgaatacca gggaaagcct
      841 ctggaaagta ttgctgaata cctgcacaaa gaaaaaaaat tcaaagtggg cattatcacc
      901 tctgtgccgc taaaccatgc aacaccggct agtttttacg tccacgagcc cacacgcaga
      961 aactatgata acatcagtca tggaataatt gagagcgggt ttgaattttt tgccggtggc
     1021 ggcttccagt ttggcaatgc agataccaca aaaaaaattt acagagattt caatgccgta
     1081 gtgcataaca tgacagagaa taattatcag ttggtttacg aagaagcgca actgacagcc
     1141 gacaatgccg cctcctttca ccacgtagtc attatagaca ctgcaatccg caacaggcag
     1201 acagcgctga aaaacgtgta tccggaagag agaaactccc tgccatttgc tctggatttc
     1261 ccggaatata aaatgcaatt agctaaatac accgaaaagg cacaaaattt tttgttcaac
     1321 gataagggat tttttatcat ggtagaaggc ggcaaaatcg actgggcaag ccacgacaac
     1381 gatgcccttg cagctataca cgaagtgaat gcctttaatg aagccataaa aaaagcttat
     1441 gaattttacc tgaagcatcc gaaaaacacc ttaatacttg ttactgccga tcatgaaacc
     1501 ggaggcttgt cgctgggcag cggacaagat gaatacggcg ctgtgaaatc aaacaaatac
     1561 gctttataca tccgcaagct gctgcaacaa aagaaatcat acatctttac tgataaagaa
     1621 agtcttaaaa agatacagga agatgcccag ataggttgga caacggatga gcatacggcg
     1681 gcaccggtgg gtatatgggc aataggaaga ggcagtgaaa atttttcggg cattatgaaa
     1741 aactcagacc tgaaaacaaa aattctgcat ctgataaagt agtaccggtc ggaaatacaa
     1801 tttggtataa catcaaacca ccagcacccg caatgccgcg ggctctatag agacttttac
     1861 cggtgtgtat ccaacaaact caccgtcgat ttcaaaaggc gccttgcccg acaacgactc
     1921 aatggaaagt tcccgggccg agtggtaatg tatttcagga tgaattattt tcttgcattt
     1981 tcgcgctttt ggtaattgtc tgaggtaatc aaggatggaa atatctccca gcaatgttac
     2041 cgatagcaat ccatcgtcag gaagagcatc gggcgcaatg ccaataccat tgccaaaata
     2101 cttgccgtta tttataacaa aatccatgac gttgccttca taagtaaaac tgtcggctgt
     2161 tacctttata tgatttttct tataggtaaa aaacgtacca ataattgccc gctggtaatt
     2221 aaaaaacgcg ctcaatcgtt ttgaataggc actttgcttt tgtaccacca cggcacccat
     2281 acccacatcg gcaacattag ccatgtagcg gctttcctcg cctccagcca cagattcgta
     2341 agtggcagaa caaacatcaa taagcctggt tttgtcgttt tggatagccc caagaaggct
     2401 ttgtgcattt tccggtacgg caacggttct tacaaaatca ttacccgatc cttttggtaa
     2461 aacgcccaaa cgaatcttca gccattgttc ttcggtgaga tgtttttttg ccttaagcat
     2521 gccattaact acttcatgca ggcagccatc gccaccaaca cttattacat gggtaaaccc
     2581 ctgctgcagg gcaacttctg ccaatcgaat accatgttgg gcatgcgtgg tgataaaact
     2641 tttcacccga ttttcctgtc cgaaatatgt ttcataagtt tgaaaaagta aatcctgatc
     2701 ttttttcctt ccgtcaataa taaaagctac gtcggccata attaaatttt ttaaaaatcg
     2761 atttgctgaa ttcgaaaagc gcaggatttt gtagtttttt agaggcactt tagtatatca
     2821 gcaatttgtt tcaaaattat tataaaaaaa ggaatggaag aaataaacaa gaatttttta
     2881 tagacaaatt gcatatcaat attatttttc actgttgccg gtaacatttt attatgtagg
     2941 tttgttaaaa atttttccat gaaacacaaa attgctctta ttggcttcgg tactgtcgga
     3001 caaggtatcg ccgagatact tcttaaaaaa aaggaatatc tgaaagataa tttcaaattt
     3061 gattatgatc tggtggcggt tgcagatttt ttttatggaa attgttacaa ccccaaaggc
     3121 cttgatacta cttacctgct gaaaacagcg aaggcaaaaa agaaattttc aaaagatctc
     3181 agtaccaaag acaatttatc gctgattaaa agttgtaatg cggatattgt ttgtgaactc
     3241 acatacaccg acctgaaaac cggcggccct gcccttggtc atgttaaggc cgcgttgtcg
     3301 tcaaagaagc atgtaataac cagcaacaaa ggtcctgctg cgttgaaata tcaggagttt
     3361 agcaagctgg ccaaaagaaa cggggtaaag tttatgattg aaggcaccgt ggtttccggc
     3421 acaccggtta tcaaccttag caaaggcccc ctggccggat gcgaaataag caaaatcaaa
     3481 ggcatactga atggtacaac caactatatg cttaccgaga tggaaaaagg tatggactat
     3541 gcctcagtgc tgaaagtggc gcaggatctg ggttatgctg aagccgatcc cacaggcgat
     3601 gtggaaggat acgatgccag aggaaaagtt tgtattttgg ccaatgtggt gatgggcgtc
     3661 cccctaaatt tcaacgatat tgcctgtaag ggaattaccg gcataactct gggtgatatt
     3721 gaaaaagcaa aaaaagaagg caagcgttgg aaactcatcg gttgtgtgga aaagaaaggc
     3781 aacaaggttg tcggttcggt aaaccccgag atggtagaat tatcgcatcc cttagccggt
     3841 gtcatgggcg ccaaaaatgc gctgaccttt accaccgata tgcttggtga tgttactatc
     3901 attggcccag gagccggaaa gacggagaca ggattctcca ttttgaccga catgcttgc
//
LOCUS       DAOSFP010000218         3862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_21621_length_3862_cov_3.827423, whole genome shotgun sequence.
ACCESSION   DAOSFP010000218 DAOSFP010000000
VERSION     DAOSFP010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21621_length_3862_cov_3.827423"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..885
                     /locus_tag="PLI16_07085"
     CDS             124..885
                     /locus_tag="PLI16_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HOH84360.1"
                     /translation="MLRTIKIVKHQGRQYIKNLQQVTLPLLNNGLPVISSNPCPAACK
                     ACVELCPSEAISKDPLTVNLGQCVFCGECVSCKEGIIRFTNNFRIATNNSSCLAVSST
                     TDHIFFDPELVRKEIIKVFGRSLKLRLVSDGSCNGCELELNATDNVNFDISRYGIEFV
                     ASPRHADGIVVTGPLVENSAKALELTWEAIPDPKILIAVGTCAISGGVFKNSKALNRG
                     FFEKHTPDLYLPGCPPHPLTIIDGLLKLTGRITAE"
     gene            1269..3062
                     /locus_tag="PLI16_07090"
     CDS             1269..3062
                     /locus_tag="PLI16_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOH84361.1"
                     /translation="MKEKIISIVESYGKDAYRLMDILSCVQSEFGCVSGEAVGVIAEQ
                     LHISEVDVIQTRSFYHFYTCRPVGKYAVYLNNSVVSCMMGMADVADEFEKQLGIRFGE
                     STTDELASLHYTACIGMSDQEPAAIINDVVFTSLTREKVKDIIAGMRAGKAVAEMVTE
                     YGDGNNAHALIKAMVKNNIKKTGPVIFDAHQTGAGLAKALGMDPKSVIGEIKESNLRG
                     RGGAGFPTGLKWDFCSRESGDKYVLCNADEGEPGTFKDRVLMTERSEMLFEGMAIGGY
                     AIGAKEGILYVRKEYKYLKAFLENTLKEMYAKNLLGKNILGKGVDFDIRIQFGAGAYV
                     CGEESGLIESCEGKRGEPRNRPPFPAQKGYKSKPSIVNNVETFCSVARILDKGGAWHN
                     ALGTKESSGTKVISVSGDCKTPGIYEIQWGMSIKEMLDMVGAEDVQAVQVAGPSGICL
                     PPSQFDRKIALEDLPTGGSMIVIGKKRDLIKDVVLNFMDFFADESCGSCVTCRSFNMI
                     LKKAIEKVVEGHAVQKDVEDMLAWSEILRKTTRCGLGQTSSNPVATTIRNFKELYDAR
                     VHDQDFLSNFDMHKAVLESCEVVGREPKVHE"
     gene            3104..3808
                     /locus_tag="PLI16_07095"
     CDS             3104..3808
                     /locus_tag="PLI16_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOH84362.1"
                     /translation="MSETVKFTIDGKECEGTKGQCIVDAATDNGIYIPVLCHMRDVVP
                     AGSCRICTVNVNGRKMAACTTPVEDGMVVENITPELTDIRKAIVEMLFVEGNHFCPAC
                     EKSGNCDLQGLGYKYQMMVPRFPYAFERRDIDATTPKIYLERNRCIFCKRCVRIIKDS
                     EGRSIFAFKGRGKTLQINIDRKLAATMSDELAQKAMDICPVGCILKKEVGFKDPIGSR
                     KYDKKPIGSDIETIHQ"
BASE COUNT         1146 a          815 c          872 g         1029 t
ORIGIN      
        1 ctcgttgcac aactggatgg cgttggcgct ggctgttcgc aacaatgaga tttccgattt
       61 tcctgtttgt aataaaagtt ttaacctgtc ctattgtggt tacgatctgt aaacttcgcg
      121 cttatgttaa gaactataaa aattgtaaaa caccaggggc gacagtatat taaaaacctt
      181 caacaggtaa cccttccgtt attgaacaac ggactgccgg tgatttcttc caatccctgt
      241 ccggctgcct gtaaagcctg tgtggaatta tgcccgtcgg aggctatatc caaagatccg
      301 ctgaccgtca acctgggaca gtgtgttttt tgcggcgaat gtgtcagttg taaagaaggc
      361 attatcaggt ttaccaataa cttccgtatt gccaccaata acagttcgtg ccttgctgtt
      421 tcatccacta cggaccacat tttttttgat cctgaattag ttcgcaaaga aatcataaag
      481 gtatttggca ggtctttaaa actgcgcctg gtttccgacg gcagttgcaa cggttgtgaa
      541 ctcgaactga atgccactga caatgtaaat tttgatatca gccgctatgg tattgagttt
      601 gtggcttcgc cgcgtcatgc cgacggtatc gtggtaacgg ggccattggt tgaaaatagc
      661 gctaaggcgc tcgaacttac ctgggaggcc atacctgatc caaaaatact tattgccgtg
      721 ggaacttgcg ctatcagcgg aggcgtattt aaaaacagca aagcgctgaa ccgtggcttt
      781 tttgaaaaac acacccccga cctttacctt cccggttgtc cgcctcatcc gctcaccatc
      841 atcgacggcc tcctgaaact taccggaaga attactgccg aatagtacct gattgaaata
      901 tttagtagcc atcaggaggt aatggatacc tctcataacc ttttccggca ggcacagtca
      961 gcgtatgaca atgtaaaacg tctttggcct gtctgcaatt tcacttttgt atttttttgt
     1021 acaaattatt attgcctgtt tacttgcttt taggcttttt ggtgcctgat tcacgaaaat
     1081 atatgtattc tgttaaatat gttttattta tttttatttt ggttaaaatt ttataaactt
     1141 aaaaaagtta gatgtgttta attaataaaa ttttcgatta attgtgatag aattaaattt
     1201 ttactaaatt tgtcccttaa aaatcatatt atgtaaaacc atcaaaatca taaacaaacc
     1261 caacatctat gaaagaaaaa attatcagta ttgtcgaatc ttacggaaaa gatgcctacc
     1321 ggctgatgga tatcctgtcc tgtgttcagt cagaattcgg ttgtgtttcc ggagaagccg
     1381 tgggtgtgat tgcggaacag cttcatattt cggaagttga tgttattcaa acccgttcgt
     1441 tttatcattt ttatacctgc cgaccggtag ggaaatatgc tgtttacctc aacaacagcg
     1501 ttgtttcctg catgatggga atggctgatg ttgccgatga atttgaaaaa caactcggta
     1561 tccggtttgg agaatccaca accgatgaat tagccagcct gcattatacc gcttgtatcg
     1621 gtatgagcga tcaggagcct gcagcaatta ttaatgatgt ggtttttacc tccctcaccc
     1681 gtgaaaaagt gaaggacatc attgccggca tgcgtgccgg aaaggctgta gccgaaatgg
     1741 ttaccgagta cggggatggc aataatgcac atgccttgat aaaggccatg gtaaaaaata
     1801 atatcaaaaa gaccggcccg gttatttttg atgcacatca aaccggagca ggactggcca
     1861 aagccctggg tatggatccc aaatcagtaa tcggagaaat taaagaatcc aaccttcgcg
     1921 gacgtggcgg cgccggattt cctaccggat taaaatggga tttctgctca cgcgaatcag
     1981 gtgataaata tgtgttatgc aatgccgatg aaggcgaacc gggtactttc aaagacaggg
     2041 tactgatgac cgaacgctcc gaaatgttgt tcgaaggtat ggctatcggc ggttatgcca
     2101 tcggggctaa agaaggtatc ttatacgtta gaaaagagta caaatacctg aaagcatttt
     2161 tggaaaatac actcaaagaa atgtatgcca aaaatctcct tggtaaaaat atactgggca
     2221 aaggtgttga ctttgacatc aggatacagt ttggcgcagg cgcctatgtg tgcggtgaag
     2281 aatcaggcct gatagaatca tgcgaaggta aacgcggcga accgcgcaac cgtcctcctt
     2341 tccctgcaca gaaaggttac aagagcaaac catccattgt aaacaacgtg gaaactttct
     2401 gttcggtggc caggatactg gataaaggcg gtgcatggca caatgctttg ggaacaaaag
     2461 aatcgtcagg cacaaaagta atcagtgtgt cgggcgactg caaaaccccg ggcatttatg
     2521 aaatccaatg gggtatgagc atcaaagaaa tgctcgatat ggttggtgcc gaagatgttc
     2581 aggctgttca ggtagccgga ccctcgggaa tttgtctgcc tcccagccag ttcgacagaa
     2641 aaatagccct ggaagatttg ccaaccggag gttccatgat agttattggt aaaaaacgcg
     2701 acctgataaa agatgtcgtg ctgaatttta tggatttctt tgccgacgaa tcctgcggct
     2761 catgtgttac ctgccgttct ttcaatatga ttctgaaaaa agctatcgaa aaagtggtcg
     2821 aaggtcatgc agtacaaaaa gatgtagaag atatgcttgc ctggagcgag atactccgta
     2881 aaaccacccg ttgtggcctt gggcagacat catccaaccc tgttgctacc acgatccgca
     2941 atttcaagga attgtacgat gcccgcgttc acgaccagga ttttctcagt aatttcgata
     3001 tgcacaaagc cgtgctcgaa tcatgcgaag tggttggccg cgaacccaaa gttcatgaat
     3061 aattcaaaaa atcagatatt aacaattata aagaaaaata aatatgagtg aaactgtaaa
     3121 attcacaatc gacggcaaag aatgcgaggg gacaaaaggc cagtgtatcg ttgatgctgc
     3181 aacagataat ggcatatata ttcccgtact ttgtcatatg agagatgttg ttccggccgg
     3241 ctcatgcaga atatgcaccg taaatgtcaa cggcagaaaa atggccgcct gtaccactcc
     3301 ggtggaagat ggcatggtgg tagaaaacat cactcctgaa cttaccgata tccgaaaagc
     3361 tattgtcgaa atgttatttg tagaaggaaa ccatttctgc cccgcctgcg aaaaaagcgg
     3421 aaactgcgat ctgcagggcc tgggatacaa ataccagatg atggtacccc gtttccctta
     3481 tgcttttgaa cgcagggata tcgatgctac cacaccgaaa atttacctcg aacgcaaccg
     3541 ttgcattttt tgcaaacgtt gtgtgcgcat tatcaaagac agcgaaggcc gtagtatttt
     3601 tgcctttaaa ggcagaggaa aaactttgca aatcaatatc gacagaaaac ttgccgccac
     3661 catgtctgac gaactggcac aaaaagccat ggatatctgc cccgtcggct gtatcctgaa
     3721 aaaagaagtc ggctttaaag atcctatcgg aagccgcaaa tacgacaaaa aacccatagg
     3781 atccgatatt gaaaccattc atcaataaaa caggaggaaa aaaaatggaa gaaacaaaga
     3841 aaacccttac ccgtcgaggc tt
//
LOCUS       DAOSFP010000219         3826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_21924_length_3826_cov_4.798462, whole genome shotgun sequence.
ACCESSION   DAOSFP010000219 DAOSFP010000000
VERSION     DAOSFP010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21924_length_3826_cov_4.798462"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="PLI16_07100"
     CDS             <1..1305
                     /locus_tag="PLI16_07100"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84363.1"
                     /translation="LDSDMLNVIKKILIPLILVILFLPMAQQHFHFVKIKKLQGFYAT
                     SPKPDTLNFRTWTDESFQKKFSKYLEDTLGFRQIMIRMNNQINYSLYKKTDARNVVVG
                     KSNCLYEEGYILDCTGSNFLGEKFWNELLRRTKLVQDTLKKAHNTDLILVLEAGKASF
                     YPEYIPDRYHAGQKTISNMDYIAKQAKLLQINHLDLNTLFCAMKDTSKYPLYATYGVH
                     WSTYGMYKAADTLARFIERLRQVAIPEIVWQGYSVTDQIKDVDFDIEATMNLLFDLPH
                     MEMCYPQIGYKSAGKVKPKLLTIGDSYYWGFINNHIVDSLFTNHQYWYYFSGIWPDMW
                     DYKIIPLELNLQQELEKQDVILVGFTELNAFYGFWGFIDEAYKIYFPNDYNKDYEAFK
                     ELKKNDLHFFRHRALAKKYQRSVQEIIDFEIKNKDTYRSGLN"
     gene            1341..2891
                     /locus_tag="PLI16_07105"
     CDS             1341..2891
                     /locus_tag="PLI16_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84364.1"
                     /translation="MKLFKDKIFLILFVIACVLNTLPILLNTFIISLDGPSHLYNANL
                     IAALISGNGAVQDFVALNNYFTNWFGHFLLILFNLVAPAWIAQKILLILLVVAVPAAF
                     AFMVCRINYSSRYLSFIIFPFSYSTFFFLGFYNFLLAVVFFFLWMAFVYKEIRTYSRI
                     EKVFIFALFLLIECSHIFVFMIALGMFAWFLAAPVLQKNKFHIKSLLHDMLVPLKTFF
                     SLAGGGLLLLAAYFLGSDRHQFEKPQNDTPWDKIVADIYEIHPLQGLSNDYHVLTAVL
                     FVVFVVITLFVATRALIARKNKTSYFLMYNALLFLVLYVVTLLFLDKGLYIQSRLLFL
                     FFLLWWTWAAIQRIPKLVQVLVIAFSLTVSVVFMNYYMKMLPYLSMRSYYAYDAGLKI
                     EKNARVFCVNYSSHWLENHLLNYLGAENTLVMYNNYECEQNFFPLKWRHPETVRNVWS
                     EYYSGSYSEVFYSNVDYIAILQENQHPSNPNDLQFLSMVKEHYSWVMTSGDEGITIYK
                     KIQKKMVR"
     gene            complement(2898..3635)
                     /locus_tag="PLI16_07110"
     CDS             complement(2898..3635)
                     /locus_tag="PLI16_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84365.1"
                     /translation="MRFFTTLLISMLLPLALVTKIHAQPLKPVITFEEDPLTHNVHVA
                     SDGQHYYTVNGGRADKGQINKFAFTGKLLDSYEIKLDMRSIMFNKKDKKFYVCTFERE
                     IFRINDLQRGSYEKVSSEMYDNGQANLAMSANAKSIYFFNNGTLKIFSFPKGVLQKEM
                     KGFDCGKDFSSGGGSVAVDGKYIYTWNADYKLIFVYDMKGQKVKSVEIEKGSYGFSLS
                     YANGLIFVADDGDYATGTWYGYNLWEK"
BASE COUNT         1049 a          800 c          780 g         1197 t
ORIGIN      
        1 ttagattctg atatgctaaa tgttataaaa aaaatattaa tcccgttgat ccttgtgata
       61 ctgtttttgc ccatggcgca acagcatttt cattttgtga aaataaaaaa actgcagggg
      121 ttttatgcga cttcgcccaa accagacacg ctcaacttca ggacatggac cgacgaatct
      181 tttcagaaaa aattttcgaa atatctggaa gacaccctgg gattccgtca gattatgatc
      241 aggatgaaca accagataaa ctatagtctt tacaaaaaaa ccgatgccag aaatgtggtg
      301 gtaggcaaaa gcaattgttt gtatgaagaa ggctatatcc tggactgtac gggcagtaat
      361 tttcttgggg aaaaattctg gaacgaactg ctgcggcgca ccaaattggt gcaggacact
      421 ttgaaaaagg cccataatac agaccttatc ctggttctgg aagccggcaa ggccagcttt
      481 tatcccgaat atatccccga caggtatcat gccggccaaa aaaccatttc aaatatggat
      541 tatattgcta aacaggctaa acttttgcag ataaaccatc tggacctgaa taccttgttt
      601 tgtgcgatga aagatacgtc aaagtatcct ttgtatgcca cctacggtgt gcattggagc
      661 acctacggca tgtacaaagc cgccgatacc ctggcacgct ttattgaacg gctgcgccag
      721 gtcgctatac ccgaaatagt ctggcagggc tattccgtta ccgaccagat caaagatgtg
      781 gattttgata ttgaagcgac tatgaacctc ttgtttgatt tgcctcatat ggagatgtgt
      841 tatccccaga ttggttataa atcggcaggc aaagttaaac ccaaactgct gaccatcggc
      901 gacagctatt attggggatt tatcaataac catattgttg actccctgtt taccaaccat
      961 cagtattggt attatttttc ggggatatgg cccgatatgt gggattataa aattatcccg
     1021 ctggaactga accttcagca ggaacttgaa aaacaagatg ttattctggt tggttttact
     1081 gaattgaatg ccttttacgg attctgggga tttattgacg aggcctataa aatttatttc
     1141 cccaacgact ataacaaaga ctatgaggca ttcaaagagc tgaaaaaaaa cgaccttcat
     1201 tttttccgcc accgggctct ggccaaaaaa tatcagcgtt ccgtacagga aatcattgat
     1261 tttgaaataa aaaacaaaga cacctaccgt tcaggtttaa attgagatat ttgtacggga
     1321 tttattgaat atgttatgat atgaaacttt ttaaagacaa aatattcctc attttatttg
     1381 ttattgcctg tgtgctgaac acgcttccaa tacttctcaa cacctttatt atcagcctcg
     1441 acggtccttc gcacctgtat aatgccaacc tgatagccgc actgataagc ggcaacggcg
     1501 ccgtgcagga ctttgtggcg ctcaacaact actttaccaa ctggttcgga cattttttgc
     1561 ttattttgtt taatcttgtt gcgcccgcct ggatagccca aaaaatattg ttaatcctgc
     1621 tggtggtggc tgtgcctgcc gcttttgcct ttatggtgtg ccggataaat tattcatcgc
     1681 gatacctgag tttcatcatt tttccttttt cctactccac ttttttcttt ctgggttttt
     1741 ataatttttt gctggccgtg gtttttttct ttttgtggat ggcctttgtt tacaaagaaa
     1801 tccgcaccta tagccggatt gaaaaagtct tcatcttcgc cttgttttta ctgatagagt
     1861 gttcgcacat ttttgtgttt atgatagcac tggggatgtt cgcctggttc cttgcggcgc
     1921 ctgtactaca aaaaaacaaa tttcatatta agagtttgct gcacgatatg ctggtgccgc
     1981 tgaaaacttt tttttcgctc gccggcggcg gcttgttgtt gcttgcagcc tattttctgg
     2041 gaagcgaccg ccatcagttt gaaaaacctc aaaacgacac gccctgggat aaaattgttg
     2101 ccgacattta tgaaatacat cccctgcagg gcctcagcaa cgattatcat gtgttaaccg
     2161 ccgtgttgtt cgttgtgttt gttgtcataa cgctttttgt ggctacacgt gcgctgatag
     2221 cccgtaaaaa taaaacctct tattttctga tgtacaacgc cctgctgttt ttggtgttgt
     2281 atgtggttac gctgttattt ctcgacaagg ggctgtacat ccaaagccgc ctgttgtttt
     2341 tgtttttcct gctgtggtgg acatgggctg ccattcaacg aattccgaaa ctggtgcaag
     2401 tcttggtgat agcgttcagc ctgacggtat cggtggtatt tatgaattat tatatgaaga
     2461 tgctgcccta tctttctatg cgaagctatt acgcctatga tgccggcctg aaaattgaaa
     2521 agaacgcccg tgttttttgt gtcaattact cttcccattg gcttgagaac caccttctaa
     2581 attacctggg ggccgaaaat actcttgtta tgtataataa ctacgagtgc gaacaaaact
     2641 ttttccccct gaaatggaga catcccgaaa cggtaagaaa cgtatggagc gaatattatt
     2701 cggggagtta ttcagaggtt ttttacagca atgtggatta tattgccata cttcaggaaa
     2761 atcagcaccc ttcaaatcct aacgacttgc agtttctttc gatggttaag gaacactatt
     2821 cctgggtaat gacttccggc gacgaaggga taaccatcta taaaaagatt cagaaaaaga
     2881 tggttcgttg attcttttta tttttcccag aggttgtagc cataccaggt tccggtagca
     2941 tagtcgccgt cgtcggctac aaaaatcagc ccgttggcat acgacaatga aaacccgtag
     3001 ctgccttttt caatttccac cgattttact ttttgtccct tcatatcata tacaaatatc
     3061 agcttatagt cggcgttcca ggtatagatg tattttccgt ccacggccac gctgccgcca
     3121 cccgaactaa aatcctttcc gcagtcgaaa cctttcattt ctttctgcag gacgcctttc
     3181 gggaaactaa agatctttaa cgtaccgtta ttaaaaaaat atatgctttt tgcgttagca
     3241 ctcattgcaa gattggcctg accgttgtcg tacatttcgg agcttacttt ctcataactg
     3301 ccacgctgca aatcgttaat gcggaaaatc tccctctcga aagtgcatac ataaaacttt
     3361 ttgtcttttt tgttaaacat gattgaacgc atgtcgagct ttatttcata cgagtcaagc
     3421 aatttgccgg taaaggcaaa cttgttgatc tggcctttgt ctgccctgcc tccatttacc
     3481 gtatagtagt gttggccgtc cgatgccaca tgtacattat gtgtcagggg atcttcctca
     3541 aaagttatca cgggttttaa aggctgtgca tgaatttttg tcactaatgc taacggcaac
     3601 agcatgctta tcaggagggt tgtgaaaaat ctcataactg aatagatttg gttaataaaa
     3661 atttaatgac tgaaaaaaca cagtaaagat aacaaaaaat gaggcctgaa aaagtttgct
     3721 gaaagaatta gaaaaaaata tttatgaaat aattggatgg catattaaaa tattttaatg
     3781 tttcaaaaat ggttaaatcc ccgcaattta ttgcggctac ttttag
//
LOCUS       DAOSFP010000220         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22029_length_3814_cov_4.249268, whole genome shotgun sequence.
ACCESSION   DAOSFP010000220 DAOSFP010000000
VERSION     DAOSFP010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22029_length_3814_cov_4.249268"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLI16_07115"
     CDS             complement(<1..466)
                     /locus_tag="PLI16_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOH84366.1"
                     /translation="MANFTHLHVHTQYSILDGASNIAKLIQKVKDTGMTAIAITDHGN
                     MYGVKEFHNIATKNQIKPILGCETYVAAKGRADKSDSDDRSGFHLILLAKNELGYKNL
                     CKLVSLAFIEGFYYKPRIDWELLAQYHEGLIASTACIAGEVPYHILNNDIEKA"
     gene            913..1479
                     /gene="efp"
                     /locus_tag="PLI16_07120"
     CDS             913..1479
                     /gene="efp"
                     /locus_tag="PLI16_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOH84367.1"
                     /translation="MATTADLRNGLCIEFNNDLYVVVEFQHVKPGKGGAFVRTKLKSL
                     TTGKVLANTFNSGTKIEVTRVERRPYQYLYKDDTGYNFMHVETFEQVNIMEELIESYD
                     LLKEGQRVEIMFHADTERPLSCELPAFVELEITYTEPGLRGDTATNTLKHATVETGAT
                     VDVPLFINIGEKIRIDTRTHTYYERVKG"
     gene            1509..2432
                     /locus_tag="PLI16_07125"
     CDS             1509..2432
                     /locus_tag="PLI16_07125"
                     /inference="COORDINATES: protein motif:HMM:NF016494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOH84368.1"
                     /translation="MKFEKSYTLKEIAGFLNGKYLGDDEHKITGINEIHMVEPGDITF
                     VDFHKYYDKALNSEASTIIINKDVTPPDGKALIISDDPFRDYNKIVARFRPFEPCAKH
                     ISDTATIGQGTIIQPGVFIGNHVTIGENCIIHPNVSIYDHTIIGNNVIIQSNSVIGGD
                     AYYFKRRPTHYEKMESCGRTILEDDVEIGALCAVDKGVSGDTIIGKGTKLDNHVQIGH
                     DTHVGKHCLIGAHSAIAGVTIIEDEVCIWAQVMINKDIVVGKGAVLLATSAIDKSVEG
                     GKVYFGSPAIEARKKWREIVTLRKIAENYKD"
     gene            2686..3516
                     /locus_tag="PLI16_07130"
     CDS             2686..3516
                     /locus_tag="PLI16_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84369.1"
                     /translation="MKKIAFFIFALGFAVSFSACKKDKGADPVSGCMDSGSLYYNPLA
                     TVDDGTCVVPEQVQKALVFQVTATDCVNCGMWGGDTLDALVANNPGKVVPIALHYGDN
                     MTPNVLRFGFSPILPSTIGTPYFKIGDVETYYYNSQVAALCAQTPVVAQTAFIYTQGS
                     ETVNVKTRTEFFTDVDGVYMLAVYVLESGIDGTSGPYVQEGGSTGYQHNYVLRASSYN
                     NSPFGETIVPSGALAGKTVDKTYDITFSKLSATSAINIATVIWKKEGSEYKFINGYYK
                     "
BASE COUNT         1147 a          699 c          808 g         1160 t
ORIGIN      
        1 ctgctttttc aatatcatta ttcaaaatat ggtaagggac ttctccggcg atgcaggctg
       61 tggaggctat caatccctcg tggtactggg cgagcaactc ccaatcgatg cggggcttgt
      121 aataaaagcc ttcgataaag gccagcgaaa ccagtttgca taggttttta tatcccagtt
      181 cgtttttagc gagcaaaatc aggtgaaatc ccgaccggtc gtcagaatca gatttgtcgg
      241 cccggccttt ggctgccaca taagtttcgc agcccaggat aggtttaatt tgatttttag
      301 tcgctatatt gtgaaattct tttacaccgt acatattgcc atggtcggta atggcaatgg
      361 ctgtcatccc cgtgtctttg accttctgga tcagttttgc gatattcgat gctccgtcga
      421 gtatagaata ttgggtgtga acatgtaaat gagtgaaatt ggccatgatt ttttttatga
      481 attgcgttgc atttttactt ttgtaaaagt agaaaatttg ggggtttaaa acactgttgt
      541 tgataaatta aaataacttt atttttgcct tgctaccaca tacactatta ggtaattaca
      601 ttaattgatg aacgcgcaat atgtaaaata atgaaataat aatattgatg atttaaaaat
      661 attcaaaaga aattatcgtg tgttacccta taataaaatt gtgttttacc tgacaaatat
      721 taaaaagagg attaatacta tgtctgcgaa ggggcctttg gtttgaccca aattcggcct
      781 gtttcagttt ttttgttgaa aatcaataaa ttattttcgt tttaccgtca attctactct
      841 tgtaaaatta ctgcttgtcg tttagatttt ttgtctattt ttgcacgaaa ttttcaaaaa
      901 aacaaaacat ttatggcgac taccgcagat ttaagaaacg gcttgtgtat agaatttaat
      961 aacgacttgt atgttgttgt agaatttcag catgttaaac ctggtaaggg cggagcattt
     1021 gtccgcacaa aattaaaaag tcttactacc ggaaaagttc ttgccaatac ttttaattcc
     1081 gggacaaaaa ttgaagtaac aagggtggaa agaaggcctt atcagtattt gtacaaagac
     1141 gataccggat ataatttcat gcatgtcgaa acattcgaac aggtaaacat catggaagag
     1201 cttatcgaat cttatgatct gcttaaggaa gggcaacgcg ttgaaataat gtttcatgcc
     1261 gataccgaac gccctctgtc gtgcgaattg cctgcctttg tggaacttga gattacttat
     1321 accgaacccg gactgagagg agatactgct accaacaccc tgaaacatgc caccgtcgaa
     1381 acgggagcta ccgtggatgt accactcttc atcaacatag gagaaaaaat cagaatagat
     1441 acccgtacgc atacttatta tgaacgggta aaaggataag ttggaagcat tggattaaga
     1501 ccgataatat gaaatttgaa aaaagctata ccttaaaaga aattgccggt tttttaaacg
     1561 gaaaatacct gggcgatgac gaacataaaa ttaccggaat caacgagatt catatggtag
     1621 aaccggggga tatcaccttc gtggattttc ataaatatta tgataaagcg ctgaattccg
     1681 aggcttccac cattatcatc aataaagatg ttacccctcc tgacggtaag gctttgataa
     1741 tatctgatga tccttttcgg gattacaata aaattgttgc ccgtttccgt ccctttgaac
     1801 cctgcgccaa acatatcagc gatacagcca ccatcggtca gggtacaatt attcaacccg
     1861 gcgtatttat cggaaatcat gttactatag gcgaaaactg cattatacat cccaatgtca
     1921 gcatttatga tcataccata attggcaaca atgtcattat acagtccaat tcggtaattg
     1981 gcggcgatgc ctattacttt aagcggaggc ccacccatta tgaaaagatg gaatcatgcg
     2041 gcaggactat tctcgaagat gatgtggaga tcggggctct ttgcgccgta gataaaggcg
     2101 tttccggcga taccattatc ggtaagggga ccaaacttga taatcacgtg cagatagggc
     2161 atgataccca tgttggcaaa cactgcctga taggtgcaca ttcggccatt gcaggtgtta
     2221 caatcatcga agacgaagta tgtatctggg cacaggttat gataaacaag gatatagtag
     2281 ttggaaaagg tgctgtgttg ctggctacct ctgccattga caagtcagtg gaaggcggta
     2341 aagtatattt tggttcgcct gccatcgaag cccggaaaaa atggcgcgaa atcgtaacct
     2401 tacggaaaat tgctgagaat tataaggatt aaaaaataat caacccaagc ggtacgcgcg
     2461 ttaactggtt tgtagcggta ccaaacgggg aaaataggtt atttggctac tctggccctt
     2521 aatgaatgcg gccaatattg attttttctc atttaacttt tttatctttt cacctggatt
     2581 gaatgtgcta agtttttcga ttttcagcca gttttttacc ccattggtcg ttatgacata
     2641 tagtgtgtta ttttgtcgaa gcatcgatta ttaatttaaa taaacatgaa aaaaatcgca
     2701 ttttttattt ttgcattagg atttgcagta agtttttcag cttgtaaaaa ggacaaaggt
     2761 gctgatccgg tatcgggatg tatggattcc ggctcattat actataaccc gttagccacg
     2821 gtggatgatg gcacttgcgt tgtgccggaa caagttcaga aagcgcttgt gttccaggtg
     2881 actgctacag attgcgtcaa ttgtggaatg tggggaggtg atactttaga cgcattggtg
     2941 gcaaataatc cgggcaaagt cgttccgatt gctttgcatt acggggataa tatgactcct
     3001 aacgtgttgc gttttggttt ctcgcctatt ctgccgtcaa caattggaac accctatttt
     3061 aaaattggag atgtagaaac ttattattat aacagtcagg ttgctgcttt atgtgctcaa
     3121 actcccgttg tggcacaaac agcttttatt tatacccaag ggtcagaaac ggtaaacgtg
     3181 aaaaccagaa ccgagttttt taccgatgtc gacggtgttt atatgttagc tgtatatgtg
     3241 ctggagagcg gtattgacgg gacatcaggg ccttatgtgc aagaaggcgg ctcaacaggt
     3301 tatcagcata actatgtgtt gcgtgcttct tcatataata atagcccgtt tggtgaaact
     3361 attgttcctt caggcgcttt agccggcaaa accgtggaca aaacttatga tatcactttt
     3421 tctaaattat cggcaacatc tgctataaac atagcaaccg tgatatggaa aaaggaaggt
     3481 tcagaatata aatttataaa cggttattat aaataacttg acttttccaa ttagataaaa
     3541 attgggaaag ctcctgcagt tattaataag ttatttattg gtgtcatttc taagactggt
     3601 gcgctgtcac tctgagtacg agcatattta tgtaactttt ttcgaaaaat acttacccaa
     3661 aaatcctgat taagcaggag tgcataatgc cataaccagg taatctattt aagcctttag
     3721 gcttacataa tttattttcg attccccgct gttaaaaccc gaaaaaaagc ccgaattctc
     3781 agtgctgaaa aaattgcaga aagtaacaaa aggg
//
LOCUS       DAOSFP010000221         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22196_length_3796_cov_3.567495, whole genome shotgun sequence.
ACCESSION   DAOSFP010000221 DAOSFP010000000
VERSION     DAOSFP010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22196_length_3796_cov_3.567495"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLI16_07135"
     CDS             complement(<1..836)
                     /locus_tag="PLI16_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH84370.1"
                     /translation="MSCNYLIKNALVVNEGEICQKDIFVIDGLIEEIAGRIDVSDDFR
                     QYYGIEILDMSGKYVMPGIIDDQVHFREPGLEYKGDIASESAAAVAGGVTSFMDMPNV
                     KPPTTTLYLLEQKYAAAAERSSANFSFYLGATNDNIGELKNFNPENNCGIKVFLGAST
                     GNMLVDDTQALQQIFGLPHLIAIHSEDETIIRANAEIYRQKYGEEVPVACHPLIRSAE
                     ACFVSTKRAVEMAEKNGTRLHILHLSTAAELDLLNNKLPLAEKKITTEVCAHHLWFND
                     TD"
     gene            complement(871..1617)
                     /locus_tag="PLI16_07140"
     CDS             complement(871..1617)
                     /locus_tag="PLI16_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOH84371.1"
                     /translation="MFFVTDSVIIIPTYNERENIEKIIRKVFSLPKEFNVLIVEDNSP
                     DGTAAIVKTLMTEFPGRLFIEERKGKLGLGTAYIHGFKWALAHNYGYIFEMDADFSHN
                     PDDLIRLYDACAKDGADMSIGSRYINGVNVVNWPMGRVLMSYYASAYVRMITRMKIRD
                     TTAGFKCYTRKVLETIELDKIHFIGYAFQIEMKFTTWKHGFKITEVPIIFTDRTEGQS
                     KMSKGIFKEAVFGVISLKIRSWFRTYKKVA"
     gene            1862..3229
                     /locus_tag="PLI16_07145"
     CDS             1862..3229
                     /locus_tag="PLI16_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84372.1"
                     /translation="MKKIYVILVAAFFGLFINAASAQVFTSGFESWTSSAPIVPTDWF
                     GTKTSFVADSISQYTTAPHGGTYACKLKNRLNTNKRLTTQNVSIDSGKTYQMTFWIKG
                     HGLLKAGYYKGVGSSGNNYVYNSTLFDTINTNTWIQKSFNLAADTTSAAAQFIILVKM
                     TQGDLEDIQVDDVNIDTVTAIIQPADTVSIYDIQYSTAANFASAYEDSTVVTGGVVTA
                     ICTQGMFIQSGVGPWNGIYIYSSPLIASSGVQQGDSVYVTGKVDEYFQCTELTNISSI
                     NIIGSGYSYQPYDITLLTEELEGVLVKITNAQVFDTPDYGMYPVTATGNIADSVKVHD
                     LCDTTLYLVVNNYYNITGPVHYSFNEYRILAIDTEETNSISETGEQSFSIYPNPVSST
                     LYINDIEGINQVNIVDLLGATVATVNVNGNNASLNVSNLQPGIYFVTLLKQNVIAGKA
                     RFIKE"
BASE COUNT         1102 a          837 c          764 g         1093 t
ORIGIN      
        1 tagtcagtat cgttgaacca caggtgatgg gcacatactt cggtggtgat ttttttttcg
       61 gctaaaggta atttattatt taacaaatca agttcggcag cggttgacaa atgcaggatg
      121 tgaagtcgtg taccattttt ttcagccatt tcaacggctc ttttggtgga gacgaaacaa
      181 gcttcggcac tacggatcaa gggatgacag gcaacaggca cctcttcccc atatttttgt
      241 ctgtatattt cagcatttgc cctgatgatg gtttcgtctt cagaatgtat ggctatcagg
      301 tggggcaatc caaaaatctg ctgcagcgcc tgtgtatcgt caacaagcat attgcccgtg
      361 gaagcgccga gaaagacctt tatgccacag ttgttttcgg gattaaaatt tttcagttcc
      421 ccaatattgt cattcgtggc gccaagataa aatgaaaaat ttgctgaaga ccgttcagcg
      481 gcagcggcat atttctgttc caataagtaa agggtggttg tgggaggttt tacgttgggc
      541 atatccataa agctggtaac tcctcctgcc acagcggctg ctgattccga agcaatatcg
      601 cctttgtatt ccagccccgg ctccctgaaa tgcacctggt cgtcgatgat gccgggcatc
      661 acatacttgc ccgacatatc aaggatttca ataccatagt attgcctgaa gtcgtcagaa
      721 acatcaatac gtccggcaat ttcttcaata aggccgtcga taacaaatat atctttttga
      781 catatttccc cctcgttgac aactaatgca ttttttatca aataattgca actcatggat
      841 aaatcaataa ctgcgaaatt atattatcgt tcaggccact tttttatagg tgcggaacca
      901 gctgcgtatt tttaaggaaa tgacaccaaa aacggcttct ttaaaaattc ctttgctcat
      961 cttagattgt ccttcggtgc ggtcggtaaa gatgataggc acttccgtga ttttgaaacc
     1021 atgtttccat gtggtaaatt tcatttcaat ctggaacgcg tagccgataa agtgtatttt
     1081 gtcaagttct atagtttcga gtactttgcg tgtatagcat ttgaaccctg cagtagtatc
     1141 acggattttc atgcgggtaa tcatccgaac ataagccgag gcataatacg acatcagcac
     1201 ccggcccata ggccagttga ccacgttgac accgttgatg taccgggaac caatggacat
     1261 atcggcgccg tctttggcgc aggcatcgta cagccggata aggtcatccg ggttgtgcga
     1321 aaaatccgca tccatttcaa agatgtatcc gtagttatgc gccagcgccc atttgaatcc
     1381 gtgtatgtaa gcagttccca ggcccagctt gcctttgcgt tcttcaataa acaagcgccc
     1441 gggaaactcg gtcatcaggg ttttgacaat ggcggcggtg ccatcgggag aattatcttc
     1501 cacgattagc acattaaatt cttttggaag cgaaaacacc ttacggatta ttttttcgat
     1561 attctcgcgt tcgttgtagg ttggaataat tattacgctg tcggtcacaa aaaaaatttt
     1621 ttaaaaaacg taacaaacct acatattatt ttgcagaaaa accataaatg acaaaaaaaa
     1681 ttgtaccaac aataagcagt acaatcaaaa aatgttgaaa aaaacaaaac ttccgtaaga
     1741 aaaacatcaa aaggttgggt atttatttgt atttttgttg tccgaaaaca taagtgatta
     1801 ttttttaaca ttattttatt aacataaaaa cctcatttta aataacttaa acaaaaaaaa
     1861 catgaaaaaa atctatgtta ttctagtggc agcattcttc ggcttgttta tcaacgccgc
     1921 ttctgcccag gtcttcacca gcgggtttga aagctggaca tcctccgccc cgattgttcc
     1981 taccgattgg ttcggcacaa aaaccagttt tgttgctgat agtatttcgc aatatactac
     2041 tgcaccgcat ggtggaacct atgcctgtaa acttaaaaac cggttaaata ccaacaaaag
     2101 gcttactact caaaatgttt ctattgattc cggcaaaact taccagatga cattctggat
     2161 taaaggacat ggtctattaa aagcaggtta ttataaagga gttggctcca gcggtaataa
     2221 ctatgtttat aatagcaccc tttttgacac gatcaataca aacacatgga ttcagaaatc
     2281 atttaacctt gcagccgaca ccacatcagc agcagctcag tttattattt tggtaaaaat
     2341 gacccaaggc gatctcgaag atattcaggt agatgacgtt aacatcgata cggtaactgc
     2401 cataattcaa cctgccgaca ccgtcagtat ttatgacatc caatattcaa cagcggctaa
     2461 cttcgcatca gcttatgaag actctacggt ggtaacaggc ggcgttgtta cagcaatctg
     2521 cacccagggt atgtttatcc agagtggtgt tggcccctgg aacggaattt atatttattc
     2581 ctccccgctg attgcctcct ctggtgttca gcaaggcgac tcggtttatg taaccggaaa
     2641 agttgatgag tattttcagt gtactgaact gactaacatc agcagcatca atatcattgg
     2701 atcaggctat tcctatcagc cttacgacat taccctgtta acagaagaac tcgaaggagt
     2761 cctggtaaag attaccaatg cccaggtatt cgacaccccc gactatggca tgtatcctgt
     2821 aacagctacc gggaacatcg ccgattcggt gaaagttcac gacctgtgcg acaccaccct
     2881 ctacctcgtg gttaacaatt actacaacat cactggtccg gtacattatt cattcaatga
     2941 ataccgtatt ctggctatcg acaccgagga aacgaatagc atttctgaaa ccggagaaca
     3001 gtctttcagc atttatccta atcctgtttc ttctacatta tacatcaacg atatcgaagg
     3061 catcaaccag gtaaatattg ttgacctcct cggagcaact gtagccaccg tcaatgttaa
     3121 cggaaataat gccagcctga acgtgtccaa cctgcaaccc ggtatttact ttgtaacttt
     3181 gttaaaacag aatgtaatag ccggcaaagc cagatttatt aaagaataag caagctattt
     3241 atttcaaaga aaaaaactgc ttcggaaacg gagcagtttt tttttgctcg ttgatttgcc
     3301 gcagcaacac atacaccccg gcaccatcca ggccgcaacg tgggcactga atactttggg
     3361 ttcaaatttt gcgaatatgt agccgtaatg tccgctgcct cggcctcggt ttgcacctcc
     3421 cttccggcca gggctttgtt ttcctcatgg tttgactatt atattttcca gtaagtttcc
     3481 gggtttaccc aaagtcgcac taagaatttt ttgagacagc cacttttaat ttatagtacc
     3541 tgtttttaac aaccatcgtt aaggtcagga atattgtgtt tatactccgg caggagataa
     3601 cctgaactat tcataattaa tcgaaatgaa attgtatgtg tgtggaccca gcgggcagca
     3661 ggtccggtaa ccccggcaaa ggaaatattt cagacgacaa atcattgccg gattaccgga
     3721 ttttcttttg tcggcccgaa gggtaaaaat tcgaatgaat ttttcaggcc aggcatcaat
     3781 agaaaaagaa attcaa
//
LOCUS       DAOSFP010000222         3782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22320_length_3782_cov_4.072444, whole genome shotgun sequence.
ACCESSION   DAOSFP010000222 DAOSFP010000000
VERSION     DAOSFP010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22320_length_3782_cov_4.072444"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1174..2682)
                     /locus_tag="PLI16_07150"
     CDS             complement(1174..2682)
                     /locus_tag="PLI16_07150"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOH84373.1"
                     /translation="MSENNFTDLNPNPLVSYLGKPSKDFTKNDLIRFIKEKGIQMINF
                     RYVGGDNRLKTMNICINNLKHLDTVLSTGERVDGSSLFSYIEAGSSDLYVIPRFRSAF
                     VDPFSNIPTLNILCSYFNKDGEPLASSPEYILHKAHNVLKEKTGLTFETMGELEYYLI
                     SEKDDLFRASDQRGYHESSPYNKFEDFRTEALQEIVRCGGVVKYAHSEVGNFSIDNLD
                     YEQDEIEFLPVDVEEAADQLVIAKWILRNLAYKYGVNITFAPKITAGKAGSGMHIHTR
                     LMKDGRNVMVDNHGISDIAKKAIAGYLTLAPSLTAFGNTNPTSYFRLVPHQEAPTNIC
                     WGDRNRSVLVRVPLGWCGANDMIKKANPLEAVPTDDFTDKQTVEFRCPDGSADIYLLL
                     AGLTVAARHGFEMENALQFAKDTYVDVNIFSEEFKDKLHKLDTLPFSCWASAEMLEKQ
                     ADIYKKHGVFSQQIIDGIVNNLKKFNDHDLRARLKDDKVGMLDLVERYFHCG"
     gene            2963..3658
                     /locus_tag="PLI16_07155"
     CDS             2963..3658
                     /locus_tag="PLI16_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84374.1"
                     /translation="MLIILMLFWLPTLLPAQDEIHDSLVRERIQYIRDHLDQGRTTAN
                     VWWYGWLGAYSAATIGQGTACFLSNDKGTRQDMVLGASTTLLGAAGQLVMPLNPGRKA
                     DILAQIPDSTPEQRLAKLICAEKMFKEIANNEKFGRSWKMHVIYGAVNISSGLITWLG
                     FKRSIWAGLGNFALNTVISEAQIWTQPARTMKDYQNYCRKYQSGINPVSYKPKPVFYV
                     SACPDGIALKILF"
BASE COUNT         1042 a          745 c          854 g         1141 t
ORIGIN      
        1 ctttttcgtc catattgatt aacaggatag attaattgta gtacaaaagt accattttat
       61 cctgtaattc tttgtattaa taaaccggct ttcgtgattc aaataaaaat tagaaaagct
      121 cttcacttat agagtaatag ttgtcgaaaa ttagatatta gtaccgcacc gctattaaca
      181 agcatttgtg ctatggcaaa catttaatcg tgctatgatt aagtacccca ttcattaata
      241 aatggtgcat aaggtggaac ctaagttaat aactcaactc tctcgtaaac cattaatttc
      301 aaagtaagtt ataaatgttg taaaaacaat aaatgtttgc tattagataa aatgtttttt
      361 ggaacggttc gaattgctgc gcagcagcaa cagtgcggtg gccagaaaat atggcggtgt
      421 cagggagtgg cctgtgtgaa ggttcgtcat atattttctt gactttttgg gtacttttgg
      481 gtcaagccaa aagtaccata aaaaagcaat atgtttttag tggaagcaag acctgcttca
      541 gcgcatggct gcgcgcaatc ttacgcccac ttccatcact aaggttcgtg taccgctatt
      601 cagggcaagt acatgttcat gtaacttttt ttccgaaaaa aagttaccaa acctgcccac
      661 catgctaaaa ccggttataa gggcgggaaa ccttgtcaaa atgtatactt ccacccgcta
      721 ttagacgaac gggaatggtt caaaccgaaa ctatgaatgt tttcatgtgc ttttttgctt
      781 gtccaaaaaa gtactaaaaa aagacctccc tgtcgaatgc ttccacccgc tctgaaaaaa
      841 accagaaaat ctaaaaaagt agtcccgaca actttgtgtc gggaaacccc gcaattagcg
      901 gtgccttttt tgattttcta agtttttttc attcacagcc cgcccacgcc gcacgacagg
      961 gaatggccac cgcaccgcta ttagcaagca tttgtgctat gacaaacatt taatcgggct
     1021 atgattaaat cccctttctt aataagggat gcaaaatgcg aaacgtaggt caataatcag
     1081 tgataagctt ttgacaacaa taccggtatc tagtatggcc ggatttgtgt caatagctta
     1141 tcactaaacg gacaatgatg ggtttttatg ccattatccg caatggaaat atctttctac
     1201 caggtccagc atccctactt tgtcgtcttt tagccgcgcg cggagatcat ggtcgttgaa
     1261 ttttttcagg ttgttgacga taccgtcgat aatctgctgt gagaagacgc cgtgtttttt
     1321 gtaaatgtcg gcttgttttt ccagcatttc ggcggaggcc cagcatgaaa acggcagggt
     1381 gtcgagtttg tgcaatttat ctttaaactc ttctgaaaaa atattgacat ccacgtaagt
     1441 gtctttagca aattgcaggg cgttttccat ttcgaaccca tgacgtgcgg ccacggtgag
     1501 tcctgccagc aggaggtaga tatcggcgga gccatcggga cagcggaact ccacggtttg
     1561 tttatcggtg aaatcgtcgg taggcacggc ttccagggga tttgcctttt tgatcatatc
     1621 gttggcgcca caccatccaa gaggtactct taccaatacg gaacggtttc tgtcgcccca
     1681 gcagatattg gtaggagctt cctgatgagg taccaggcgg aaataggagg tggggttggt
     1741 gtttccgaat gcggtgagcg agggagccag tgtcaggtag ccggcaatgg ccttttttgc
     1801 tatgtcgctg atgccgtgat tgtccaccat cacattgcgg ccgtctttca tcaggcgggt
     1861 atgtatgtgc ataccgctac cggctttccc tgctgtgatt ttcggggcaa aagtgatgtt
     1921 gacaccgtat ttataagcaa ggttacggag tatccatttg gcgattacca gctggtcggc
     1981 cgcttcttcg acatctacgg gcaaaaactc tatttcatcc tgttcataat ccaaattgtc
     2041 gatagaaaag tttcctactt cggaatgggc atattttacc actcccccgc aacgtactat
     2101 ttcctgaagg gcttcggtgc ggaaatcttc gaatttattg taaggcgagg attcatggta
     2161 gcctctttgg tcgctggccc tgaaaagatc gtctttttcg ctgatcaggt agtattccag
     2221 ttctcccatg gtttcgaagg taaggccggt tttttctttg agcacgttgt gtgccttgtg
     2281 cagaatatat tccggtgaag aagcaagggg ttcgccgtct ttgttgaagt acgaacataa
     2341 tatgtttaac gtgggtatgt tgctgaacgg atcaacaaat gcagacctga aacggggaat
     2401 aacatacagg tcgctggaac ctgcttcaat gtatgagaac aggctggaac catccacccg
     2461 ctcaccggtg gataagacgg tgtcgaggtg tttgaggttg ttgatgcaga tattcattgt
     2521 tttcaggcgg ttgtctcctc cgacatacct gaagttgatc atttgaattc ctttttcttt
     2581 aataaacctg atcaaatcgt ttttggtaaa atcttttgat ggttttccaa gataacttac
     2641 caggggattt gggtttaaat ccgtgaaatt gttttcgctc atgtctgttt tttttagtta
     2701 ataatcaggt gtcaaactta ataaatattt tttagaaaca aatttctttt gaaaaagagt
     2761 aatgatttca ttaaaaaaag cgcttgaaaa cactgaattt ttaacaaaaa cagaaagcaa
     2821 tgaattttcg taaattgaat tatctttgtt atgggtgaaa aaagtgggtt gagttgaaac
     2881 gagtttggat tttgttttta ttgaacttaa ataatgcagt gggatgattc gttggtcaag
     2941 gaaaaagagc aaattaaaaa ctgtgcttat tattttaatg cttttttggc taccaactct
     3001 tcttccagca caggatgaaa ttcacgattc gcttgtccgt gagcggattc aatatatccg
     3061 ggaccatctg gatcagggaa gaacaacggc aaatgtttgg tggtatggat ggttgggggc
     3121 atacagtgcg gcgaccattg ggcagggcac agcctgtttt ctgagtaacg ataaaggaac
     3181 gcgacaggat atggtgttgg gtgcatcaac tacgttattg ggagcagcag ggcagctagt
     3241 catgccttta aacccgggac gtaaagccga tatcctggct caaatccctg attccactcc
     3301 tgaacagcgg cttgcgaaac ttatttgcgc agaaaaaatg tttaaggaaa ttgcaaataa
     3361 tgaaaaattc ggaaggtcct ggaaaatgca cgtcatttat ggcgccgtga acataagcag
     3421 cggactgatt acctggcttg gatttaagcg ttccatatgg gccgggcttg gaaattttgc
     3481 cttaaacacc gtcatttctg aggctcagat ttggactcaa ccagcccgga cgatgaaaga
     3541 ttatcaaaac tattgcagaa aatatcaatc agggataaac ccggtttcat ataaaccgaa
     3601 acctgtgttt tatgtgagcg cctgccctga tggaattgcc ttaaaaattt tgttttgaca
     3661 gactactttt atcggtatta agaaaaaggg ctcagaaaca attgaatttt tttcttactt
     3721 ctatatattt tttgtatttt tgcgctcctg aaaataccta ctcttcgtag ctcagctggt
     3781 ag
//
LOCUS       DAOSFP010000223         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22414_length_3773_cov_3.665143, whole genome shotgun sequence.
ACCESSION   DAOSFP010000223 DAOSFP010000000
VERSION     DAOSFP010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22414_length_3773_cov_3.665143"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..1521
                     /locus_tag="PLI16_07160"
     CDS             82..1521
                     /locus_tag="PLI16_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84375.1"
                     /translation="MALFVVDYYLKRKLNLKLILEKLPFLGLSVLFGLLAVFSQSEKG
                     AIQDLTSMFSLFERLLIVCHGTMIYLWKLFVPVKLGAMHPYPDKLDGMFPLIYYIAPF
                     VILLVVAGIVYSRKYGRHYIFGALFFLVNIALVLQIVPVGGAAMAERYTYVPYIGLFF
                     ILGKLYDNVIRSTDTVFRRIKPLLTVLVLIFILFLSVLTFVRIGKWKNGEVLMRDLTK
                     VYPYLPFAYNNLGYYYYHWEKNYDKALREYTTAIKADSNYYQAWSNRGVVYNNMEKHE
                     EAVRDFTICLSLDPENNDGLIGKANSLSALLRFEEALPFYNQYLLKKPGDANAVLKRG
                     TAFVQLGRYKDALTDLEKSKELKPDNYEVYYWLGLYYYKNEQYAVALENLDKSISLNQ
                     KKSDIYSWRGLARYKLKQLDGAIEDYTVAIQMNPDDAAAYVNRSVANFDKGNYTQAWE
                     DINTAGRMRYPLDKPYFMKLEAIVMKNKK"
     gene            complement(1566..2651)
                     /locus_tag="PLI16_07165"
     CDS             complement(1566..2651)
                     /locus_tag="PLI16_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOH84376.1"
                     /translation="MNQKIYSVITGTGSYVPSKKVSNEDFMHHEFFDSDGAKLKNTNR
                     EIIDKFQEITTIAERRYLSDDLVTSDIAFYAAAEAIATANIDKESLDYIIVAHNFGDI
                     KANTNRTDIVPCLAARVKHKLAIQNPKTVVYDLPFGCPGWLQAMIQADYYLRSGDAKK
                     ILIIGAEVLSRVCDPHDRDSLLYADGAGAAILEARESAEPLGILAHNTRSDTLLYANM
                     LFMGKSNNPAYTPKDNLFLKMNGRKLYQYALETVPLAIKECLEKCSIPLLAIKKTLIH
                     QANGKMDDAIIKRLFGLFGLKEIPENFMPMSISWLGNSSVATLPTLLDLILKKLLPPH
                     RIDGGDLLVFASVGAGMNINAVVYKMP"
     gene            complement(2859..>3773)
                     /locus_tag="PLI16_07170"
     CDS             complement(2859..>3773)
                     /locus_tag="PLI16_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOH84377.1"
                     /translation="TGFRTDQGIVALLSGFPAQPRTAIIKNFEKFDKLPNLIKNLESL
                     GYYTSFFYGGDLKYANTETYLQLAGTNQIEGKYDIPHTRNTDWGAYDEDVFAYQLNKL
                     HEMRQPFFSVVLSLTNHEYFVADVEKVFPGKTENILFHNTAHYTDRCLYEYLQQAKKA
                     GWYNNTLFIITADHAHKHPLERAYNIPERHHIPLLLYGGALKPEYRGKTSEKIISHVD
                     IAPTLLAQLKGDNKEFTWGKNIMNKYSRSFAFYAFDDGFGFITDSADIIYDHNLKAFS
                     DVKIKINGCKTQNNQQQGKAYLQLLFQK"
BASE COUNT          983 a          748 c          875 g         1167 t
ORIGIN      
        1 agtattttat ctatgctttg atattctcaa tagtttttct ttttatcgct gctgtcgaag
       61 tctgcagcgg tgtctttgcc cgtggctctt ttcgtggtgg attattatct gaagcgtaaa
      121 ctaaatctta aactgattct ggagaagctt ccgttcttag gtctttctgt tttgtttggg
      181 ttgctggcgg ttttttctca atccgaaaaa ggcgctattc aggacctgac atccatgttc
      241 agcttgtttg aacgcttgct gatagtgtgt catggtacta tgatttacct gtggaagctc
      301 tttgtcccgg taaagcttgg tgctatgcat ccctatcccg acaaactgga cggcatgttt
      361 ccacttattt actacatcgc gcctttcgtt atattgctgg ttgtggcagg aatagtctat
      421 tcaaggaaat acggcaggca ttatattttt ggagcattgt ttttccttgt caacattgcc
      481 ctggtgctgc agattgtgcc tgttggcggc gctgctatgg ccgaacgata tacgtatgtg
      541 ccttatatcg gcttgttttt tattcttgga aaattatatg ataatgtgat tcgctctaca
      601 gacactgtat tccggcgcat caaacctctg ctgaccgtac ttgtgttgat tttcattctg
      661 ttcttgtctg tgctgacttt cgtgcgtatc ggcaaatgga aaaatggcga agtgctgatg
      721 cgcgacctca cgaaagtata tccctatctg ccttttgcat acaacaacct cggatattat
      781 tattatcact gggaaaaaaa ctacgacaag gcgctacggg aatataccac ggctataaag
      841 gccgattcaa attattatca ggcatggagc aaccgcggcg tggtatacaa taatatggaa
      901 aaacatgaag aagcagttcg tgatttcact atctgtctgt cgctagatcc cgaaaataac
      961 gacggtctca ttggcaaggc caactcgttg agtgctttgt tgcgttttga ggaagccctg
     1021 cctttttaca atcagtattt gcttaaaaag cccggcgatg ccaatgcggt gctgaaaaga
     1081 ggaacagcct ttgtacagtt gggacggtac aaagatgccc tgactgacct tgaaaagagc
     1141 aaagaactca aacccgacaa ttacgaagtg tattactggc ttggattgta ttattacaaa
     1201 aacgaacagt atgccgtcgc gctcgaaaac cttgataaat cgataagtct gaatcaaaaa
     1261 aaatcagaca tctattcgtg gcggggactg gcacgttata agctgaaaca gcttgatggc
     1321 gctattgagg attatacagt agccatacag atgaatcctg acgatgctgc ggcttatgtg
     1381 aacaggtcgg tggctaattt tgacaaaggt aattatacac aggcctggga agacatcaat
     1441 actgccggcc gaatgcgtta cccgctcgac aagccttatt ttatgaaatt agaggctatc
     1501 gtgatgaaaa ataaaaagta atgtcagtat ggcctcctaa atgaccaaat tcgtgttcag
     1561 cattttcagg gcattttata tactacggca ttgatattca ttcccgcgcc tacggaggcg
     1621 aagaccaaaa ggtcgccacc atcaatcctg tggggtggca aaagtttttt caggatgaga
     1681 tcaagcaggg taggcagcgt ggctacagat gaattgccga gccatgagat ggacattggc
     1741 ataaagtttt caggaatttc tttcagtcca aacaacccga agagcctttt aataatagca
     1801 tcatccattt taccgttggc ctgatgtatc agtgtttttt ttattgcgag gagcggtatg
     1861 ctgcattttt caagacattc tttgatggcc agaggtaccg tttctagtgc atactgataa
     1921 agcttccggc cattcatctt gagaaacaaa ttgtcttttg gggtgtatgc agggttgttg
     1981 gattttccca taaacagcat attggcgtat aacaaggtat ccgaccgcgt attgtgggcc
     2041 agtattccca gtggttcggc actttcccgg gcttcgagaa tggcagcgcc ggctccatcg
     2101 gcataaagaa ggctgtccct gtcgtgtgga tcgcaaaccc tcgacagtac ctcggcaccg
     2161 ataataagga ttttttttgc atcgcctgaa cgcaggtaat aatcggcctg tatcattgcc
     2221 tgaagccagc ccggacagcc aaaaggcaga tcgtaaacca cagttttggg gttttgaata
     2281 gccagcttat gtttcacccg ggctgccaga caggggacaa tatcagttct gttggtattg
     2341 gcttttatat caccaaaatt gtgggcaaca ataatataat ccaggctttc tttgtcgatg
     2401 ttcgctgtag cgatggcttc agcggcggca taaaaggcta tgtcggaagt aacaaggtcg
     2461 tctgaaaggt aacgtctttc cgcaatggtg gtaatttcct gaaatttatc aataatttcc
     2521 cggttggtat ttttgagctt tgccccatca gaatcaaaaa attcgtgatg cataaaatcc
     2581 tcattgctga ccttctttga tggaacataa cttccggtac ctgttatcac gctatagatt
     2641 ttctgattca tggttgatga tgtgttggtt aatgaaatta atggtaatca cgtaagtatg
     2701 aatagcatta attttttaaa tagctatgtg tatttattga taaataattt gtaaagataa
     2761 taatattttc tgaataaatg aattgtaatc cgcaacagca ataatacggg gtatccggta
     2821 tgatttccga cctggtgcag tatcagttgt aattcatttt atttctgaaa taaaagctgt
     2881 aaataggctt tgccttgctg ctgattgttt tgagtcttgc agccattaat ttttattttg
     2941 acatcagaaa aggccttgag gttatggtca taaatgatgt cggcgctgtc agtaataaag
     3001 ccaaatccat catcaaaggc ataaaatgca aaactgcggc tatacttgtt catgatattt
     3061 ttcccccagg tgaattcctt attatcgccc ttcagttgtg ccagcagggt gggagcgata
     3121 tccacgtgag aaatgatttt ttcggatgtt tttcccctgt attcaggctt tagtgcgcct
     3181 ccgtataaca acagcgggat atgatgccgt tcgggtatat tataggctct ttccaggggg
     3241 tgtttatgtg catggtcggc ggtgatgata aacagtgtgt tgttgtacca tccggctttt
     3301 ttggcctgtt gaaggtactc gtaaaggcag cggtcggtgt aatgggctgt attatgaaaa
     3361 agaatatttt ctgtttttcc cggaaatact ttttcaacat cggcaacaaa gtattcgtgg
     3421 ttggtaaggc tcagtacaac cgagaagaag ggttggcgca tctcatgaag tttattcagt
     3481 tgataggcaa aaacatcttc atcgtaggcc ccccagtcgg tgttacgggt atgcgggatg
     3541 tcgtatttgc cttctatctg gttggttccg gccaattgca ggtacgtttc ggtattagca
     3601 tatttcaggt cgcctccata aaaaaatgaa gtataatatc ccaatgattc taaatttttt
     3661 atcaggttgg gcaacttgtc aaacttctcg aaatttttga tgatagctgt cctgggttga
     3721 gcaggaaacc cgcttagcag agctactatg ccctggtcgg tgcggaaacc ggt
//
LOCUS       DAOSFP010000224         3763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22505_length_3763_cov_4.962783, whole genome shotgun sequence.
ACCESSION   DAOSFP010000224 DAOSFP010000000
VERSION     DAOSFP010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22505_length_3763_cov_4.962783"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1811
                     /locus_tag="PLI16_07175"
     CDS             360..1811
                     /locus_tag="PLI16_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOH84378.1"
                     /translation="MEPIYILLCFIAYTIMLFVITWITSRRTNNDSYYVGNKSSPWYV
                     VAYGMIGASLSGVTFMSVPGLVGTQQFTYLGLVFGFLIGYTVIATVLLPLYYRLNLTS
                     IYGYLNQRFGFWSYKTGAFYFLLSRVVGASFRMFLVVNVLQVFVFDYWGVPFWLVVSV
                     FIILIILYTFEGGVKTIIWTDTLQTTFMLLGVILSIYFISNELGIGLGEIFTKISDAG
                     YTRVVETDWHSKNFFLKQFFSGAFIAIVMTGLDQEMMQKNISCRNIKEAQKNMFTFSG
                     ILVIVNIMFLTLGAVLYMYASAKGIGIPTKTDNLFPEIAFNHLGPIAGLVFMIGLVSA
                     AYPSADGALTALTTSFCVDFLGFKDKSKLDEKQKKKVRYAVHLSFAAILLIVILIFRA
                     LNNEAVINAIYTAAGYTYGPLLGLFAFGLFTKYAVKDGYVWIVAIISPVLCYFLSMYS
                     EILFNGYKFGFELLILNGLLTFTGLFILRKRTS"
     gene            1808..2443
                     /locus_tag="PLI16_07180"
     CDS             1808..2443
                     /locus_tag="PLI16_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84379.1"
                     /translation="MIKKKSHIGKLIEQGEHQCLDFKFEISDSRKIARSISAFANTDG
                     GILLIGVKDNGKIAGVRSEEEVYMAEAAAQMFCKPPMTLKINQYLEEKKTVLEIIVPK
                     LTGEICYAQDYNGKWLAYVRVEDKNFQASQVHLRVWKRKLKAYPTHITYTEKEKKLLD
                     YLTTNPFITLREFCGLAGIKKNSAETILVNLMMLDIIEIFYTEQAVLYRLK"
     gene            2509..3537
                     /locus_tag="PLI16_07185"
     CDS             2509..3537
                     /locus_tag="PLI16_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOH84380.1"
                     /translation="MQKKIFGSLLIIFLGLKALGQTTGAENLGLVNWIDIKTAQELNK
                     TNPKPILIDVYTDWCGWCKHMMKTTFSDQGLANYINTYFYPVRFNAETKDTVEFQEKK
                     YANKNTGNRSPNDLAVILLEGKMSYPTLVFYNNNYKFKMLAPGYLKVKDIEPILVFTV
                     EHIFNTTAVKDFQQYYYKAFYPDSGFVNKDTVRWIKSFNDLKLADTSQKKKTLVFLNA
                     AWCNGGRVMLRSTFNNSIVTKYINDHYNAVLLDPETSDTIKYQNQVFVKTLENTGLHP
                     FLHYILGERIVMPSVLFFDEDLKFVSHAGQYLTPEALYPVLVYIFENHYKNKTWDEFI
                     KTFIIPVK"
     gene            complement(3548..>3763)
                     /locus_tag="PLI16_07190"
     CDS             complement(3548..>3763)
                     /locus_tag="PLI16_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84381.1"
                     /translation="FVDAAAMNQSKMAKQKKLPGAAEVAEFGYQAMLKGKPVAVHGFK
                     NRLLTIAQGLAPRKLVVNMTRKLIETR"
BASE COUNT         1084 a          855 c          771 g         1053 t
ORIGIN      
        1 tcagaaaata tttgaaaatc ttcaaaggcc ccgcagattg cggggtttcc cgacgttcgc
       61 cgtcgggacc actttgattt gattttcaat attttctgag agcgggcaga agcattgtct
      121 gggctgtcct tttctttggt tcgtttcttt tggacaggca aaagaaccga ccgtagggag
      181 ctcggcgaag ccaaatgaac gcccaaatcc aaaacaaaat cattttaaaa gttagatgat
      241 ttattctact ttttcgaagt tttcaaatcc gggttaatca aacgtcttat gttcataact
      301 aaataatatg ccacctacgg ccatgtttgc taaatgctaa ttttgcataa aatcacaaca
      361 tggaaccaat ttatatactg ctttgtttta tcgcttacac catcatgtta ttcgttataa
      421 cctggattac atcgcgcagg accaacaacg attcctatta tgtgggcaat aaaagttcgc
      481 cctggtatgt ggtggcttac ggcatgattg gcgcttcgct gagcggggta acctttatgt
      541 cggtgccggg cctggtaggc acgcaacaat ttacctatct gggcttagtc ttcggcttcc
      601 tcatcggtta taccgtaatt gccacggtgc tgctgccctt gtattaccga ttaaacctca
      661 catccatata cggctatctg aatcagcgct ttggtttctg gtcgtataaa accggagcat
      721 tctattttct cctttcaaga gttgtcgggg cttcattccg tatgtttttg gtagtgaatg
      781 tattacaggt ttttgttttt gattactggg gcgtaccctt ctggctggtg gtttcggttt
      841 ttattatcct gattattcta tacacttttg aagggggtgt gaagaccatc atttggaccg
      901 atacgctgca aaccaccttt atgctgctgg gagtaatatt atcgatatat tttatctcca
      961 acgaactcgg catcggcctg ggtgaaatct ttaccaaaat aagcgatgcc ggatacacca
     1021 gggtggtgga aaccgactgg cacagcaaaa acttttttct gaaacagttt tttagcgggg
     1081 ctttcatagc catcgtaatg accggcctcg accaggaaat gatgcagaaa aatatcagtt
     1141 gccgcaacat caaagaagcc caaaaaaata tgtttacctt cagcggcata ctggtgattg
     1201 tgaatatcat gtttttgact ttaggcgccg tattatacat gtacgcatcg gcgaagggca
     1261 ttggcattcc aacaaaaacc gacaatttat tccccgaaat cgcttttaat cacctgggcc
     1321 ccattgccgg gctggtattt atgatagggt tggtatcggc ggcctatccc agtgccgatg
     1381 gggcgctgac tgcactgaca acatcctttt gtgttgattt tcttgggttt aaagataaaa
     1441 gcaaactcga tgaaaaacaa aaaaagaaag tgcgttatgc ggttcacctg agctttgctg
     1501 ccatactgct gatagtgata ctcattttca gagcactgaa taatgaagcg gtcatcaatg
     1561 ctatttatac cgcggcagga tatacctatg gccccttact cggacttttt gcctttggcc
     1621 tattcacaaa atatgctgtt aaggatggct atgtatggat agtcgccatt atttcccccg
     1681 tgctgtgtta ttttttgagt atgtactcag aaatactttt caacgggtat aaattcggct
     1741 ttgagctttt aatactcaac ggcttgctga cattcaccgg cctgtttatt ttacgcaaaa
     1801 gaacatcgtg ataaaaaaga aatcccatat aggaaaactc attgaacagg gcgaacatca
     1861 atgccttgac ttcaagtttg agatctccga ctcacgaaaa atagcacgaa gcatctccgc
     1921 atttgccaat accgatggcg gtattctatt gatcggagtc aaagacaacg gaaaaattgc
     1981 cggcgtccgc agcgaggaag aggtatatat ggccgaagcc gccgcacaga tgttttgcaa
     2041 gccgcccatg acactgaaaa tcaaccaata cctcgaagag aaaaaaaccg tgctcgaaat
     2101 cattgttccc aaactgaccg gtgaaatatg ctatgcacag gattacaacg gtaaatggct
     2161 tgcctacgta agagttgagg ataaaaattt tcaggcatca caggtacatt tgagggtatg
     2221 gaaacgaaaa ctgaaagcct atcccacaca tattacctac accgaaaaag aaaaaaagct
     2281 cttggactac cttaccacca acccgtttat tacactgcgg gaattttgtg gcctggcggg
     2341 aattaaaaaa aacagtgccg aaactatcct ggtaaacctg atgatgctcg acatcatcga
     2401 aattttttat accgaacagg cagtacttta ccgcctgaaa taaaggtttt gcgtatcttt
     2461 gttttcacaa aaaattatct taaaaaaccg aaggaataat tttccgccat gcaaaaaaaa
     2521 atctttggct ccctcctcat cattttcctt ggcttaaaag ctttaggcca gaccaccggt
     2581 gccgaaaacc tgggactggt aaactggata gatataaaaa cggcacagga acttaacaaa
     2641 accaacccca agcccatact aatcgacgtt tacaccgatt ggtgcggctg gtgcaaacac
     2701 atgatgaaaa ccactttttc tgatcaggga ttagccaatt acatcaacac ctatttttat
     2761 cccgtgcgtt tcaacgccga gaccaaagac acagttgaat ttcaggaaaa aaaatatgcc
     2821 aacaaaaaca ccggaaaccg ctcgcccaac gacctggcgg ttatcctgct cgaaggaaaa
     2881 atgtcctatc ctaccctggt gttttacaac aacaattaca aatttaaaat gctggctccc
     2941 ggctacctca aagtcaaaga tatcgagcct atcctggtct ttaccgtcga acatattttc
     3001 aacactactg ccgtaaaaga ttttcaacaa tattattaca aggcctttta tccggattcc
     3061 ggctttgtca ataaggatac cgtgcggtgg atcaaatcgt ttaacgatct gaaactggcc
     3121 gacactagcc aaaagaaaaa aacactggtg tttttgaatg ccgcatggtg caacggcgga
     3181 agagtaatgc tcagaagcac ctttaacaac agtattgtaa caaaatatat caatgaccat
     3241 tacaacgcgg tattgcttga cccggaaaca agcgatacga taaaatacca aaaccaggtt
     3301 tttgttaaga cactggaaaa tacggggctt cacccctttc tgcattacat tcttggcgaa
     3361 cgcatcgtta tgccgtcggt attgtttttt gatgaagacc tgaaatttgt ctcccatgcc
     3421 ggacaatacc tgacaccgga agctttatat cccgtgctgg tgtatatttt tgaaaatcat
     3481 tataaaaata agacctggga cgaatttatc aaaacattta tcatccctgt gaaatagcag
     3541 cctgcaccta tctcgtctca ataagctttc gggtcatatt caccaccagc ttgcgcggcg
     3601 ccaggccctg cgctatggtc agcagccggt ttttaaagcc gtgaacagcc accggcttcc
     3661 ctttcaacat tgcctgatag ccaaattcgg ccacttcggc agcgcccggc agtttttttt
     3721 gtttggccat tttactttga ttcatggcag ccgcatctac aaa
//
LOCUS       DAOSFP010000225         3752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22593_length_3752_cov_4.103057, whole genome shotgun sequence.
ACCESSION   DAOSFP010000225 DAOSFP010000000
VERSION     DAOSFP010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22593_length_3752_cov_4.103057"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLI16_07195"
     CDS             <1..634
                     /locus_tag="PLI16_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH84382.1"
                     /translation="PSVCLGSSEVKLLDLVNSYATIVADGLYEDPVLVTRIENREGKI
                     LWEYKPERKQVIDYETAFLMQEMLKAGVKERGSTVQNLWSYNIFKDGTEFGGKTGTSS
                     NHSDMWFVGVSPKLAAGVWVGGIRRSIHFRPGSRIGEGGHAALPMFGYFMEKVMNDES
                     LKAYRQKFPGPKQKISKNYNCVTSIPDSILRAVDSILNGVDSVPAVEIPQ"
     gene            complement(710..1795)
                     /locus_tag="PLI16_07200"
     CDS             complement(710..1795)
                     /locus_tag="PLI16_07200"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOH84383.1"
                     /translation="MKKFSLFLLAVVFSSFLSAQEYFALVRMGKDWGFVDKKGSYVIN
                     PQFDVAKSFSNGLARVFQFGNWGYIDTKGTYVINPQFEQAEDFSEGLAKVRKFKQWGY
                     IDKTGVFVVNPQFDGAEDFSGGLARVRKGMQWGLIDKKGTFVVNPQFDFLGEFHDGLA
                     KALKAKQWGYVDKTGTWIVNPQFDDAEDFFGGLARVRKGMSWGYIDKTGTYVINPQFE
                     GAGDFYGGLAKAKKMKQWGYIDKKGTWVINPQFDLAEDFNAGMARVNKFREWGFIDIK
                     GTYVINPQFNEAFDFYEGLALVRKGPQWGYIDEKGMYLINPQFEGAKSFDNGLARIRK
                     GTKWGFIDKKGTIIITPQFDNAEDFSY"
     gene            1969..2661
                     /locus_tag="PLI16_07205"
     CDS             1969..2661
                     /locus_tag="PLI16_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84384.1"
                     /translation="MKKICFVVLISLVSFLATAQDVILKNNGKDIKAKVIDANNEQIK
                     FHIFSENDKLPCSIKKQLKQAQKQNQPTDNLVLTVPKKEVFMIQYENGKNDIYKDKVG
                     DRTVKNILNFIDVNDNAEAYKMYRSGYKRYSLSRGLIGAGGGLLVFGSIAMLVSATDG
                     KKDNTNLGASGVYMGAGCAFLAAGLPLYFSAKKKMDTAFDMYVQSLKTAQSNYRMDFG
                     LTQSGIGMQMKF"
     gene            2785..3384
                     /locus_tag="PLI16_07210"
     CDS             2785..3384
                     /locus_tag="PLI16_07210"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOH84385.1"
                     /translation="MTILNFLTSDAPVFTWVILPLLIFCARIFDQSVGTLRMIFVAKG
                     FKKWAPVFAFFESLIWLLAIGEIMKHLDNPLCYIAYAGGYAMGNYVGMLLDEKMSIGN
                     VIIRVMLKSEAADLIIRLRELNYGITIMNAEGATGKVKVLLSIIKRKDLVEFIHVLNQ
                     YNPQTFYTIEEVRSVNEGVFRASSKKHIFRFHQMIRKHK"
BASE COUNT         1155 a          802 c          751 g         1044 t
ORIGIN      
        1 gccttcggtg tgccttggct ccagcgaggt taagctgctc gacctggtaa attcttatgc
       61 taccattgtg gctgatggat tgtatgaaga ccctgtcctg gtaacacgca tcgaaaaccg
      121 tgaaggcaag atactttggg aatacaagcc cgaaagaaaa caggttattg actacgagac
      181 cgcatttttg atgcaggaaa tgctcaaggc gggtgttaaa gaaagaggct ctaccgttca
      241 gaacctttgg tcgtataata tttttaaaga cggtactgag tttggaggga aaacgggcac
      301 gtcgtcaaac cacagcgata tgtggtttgt gggcgtttcg ccaaagctgg ctgccggagt
      361 ctgggtgggt ggaatccggc gcagtataca ttttcgtccg ggaagccgga taggcgaagg
      421 cggccatgct gctttaccca tgtttggcta tttcatggaa aaagtcatga atgatgaatc
      481 gctgaaggca taccgtcaaa aattccccgg gcccaaacaa aagatcagta aaaactataa
      541 ttgtgtcacc tccattcctg attcgatatt aagagccgtg gattcaatat tgaatggcgt
      601 ggattcggtg cctgcggtgg aaatccctca ataaaaaaag taacctactt ataaaaaaaa
      661 agcatgaaaa aattccatgc ttttttttta taattcaggt aactgatttt taataggaaa
      721 aatcctcggc attatcgaat tgcggggtta tgataatcgt acctttttta tcaataaatc
      781 cccacttggt ccccttacga atacgggcca aaccgttatc aaaacttttt gccccttcga
      841 actgtggatt aatcagatac atgccttttt catcaatata tccccattgc ggacctttgc
      901 gtaccagcgc aagcccttca taaaaatcaa acgcttcgtt aaactgcgga ttgatcacat
      961 aagtaccttt gatatcaata aagccccatt ctctgaactt attgacacgg gccatgccgg
     1021 cgttgaaatc ctcagccaaa tcaaactgcg gatttattac ccaagtacct tttttatcga
     1081 tatatcccca ttgtttcatt tttttggctt tcgccagacc tccataaaaa tcgccggcgc
     1141 cttcaaactg cgggttgata acgtaagtgc ctgttttgtc gatataaccc caggacatgc
     1201 ccttacgcac gcgggccagg ccgccaaaga aatcctctgc atcgtcaaac tgagggttta
     1261 caatccaggt gccggtctta tccacataac cccattgttt ggcttttaaa gctttggcta
     1321 agccatcgtg aaactcaccc aaaaaatcaa attgaggatt aaccacaaaa gtacctttct
     1381 tatcaataag gccccattgc atacctttac gcacccgtgc tagaccaccg gaaaaatctt
     1441 ccgccccgtc gaactgcgga ttgacaacaa aaacacccgt tttatctata taaccccatt
     1501 gtttaaattt tctaaccttg gccagccctt cagaaaaatc ttcggcctgc tcaaactgcg
     1561 gattgataac ataggtgcct ttagtatcaa tatatcccca attgccaaac tgaaacaccc
     1621 tggcaagacc gttggaaaaa cttttggcaa catcaaactg cggattgata acgtacgaac
     1681 cttttttatc cacaaatccc cagtccttgc ccatacgtac taacgcaaaa tattcctggg
     1741 cagaaagaaa actgctgaaa acaacagcca acaagaataa agaaaatttt ttcatgatga
     1801 tttattttat gtttatgcaa aaataacaat aaatttttta ttatttcaac aagggttgtc
     1861 attgtccaat ttccagaagc tttcatagca aatttcagtt ttcaggcatt ggataacact
     1921 ggcaatttta ttgtatattt gtgccctgaa ataaaaattg ttttaatcat gaaaaaaata
     1981 tgttttgtag tgcttatcag ccttgtttcg tttttggcaa ctgctcagga tgtgattttg
     2041 aaaaacaacg gaaaagacat caaggcaaag gtcattgatg caaacaatga gcaaattaaa
     2101 tttcatatat tcagcgaaaa cgacaagctg ccctgtagca ttaaaaaaca gctgaaacaa
     2161 gcccaaaaac aaaaccaacc taccgacaat ctggtattaa ccgtacctaa aaaagaggtc
     2221 tttatgattc agtacgaaaa tggcaaaaac gatatataca aagacaaagt cggcgaccgg
     2281 acggtaaaaa atattttgaa tttcattgac gtaaatgaca atgctgaagc ttataaaatg
     2341 taccgtagcg gctacaaacg ctattcatta agccgtggac tgataggtgc cggcggcggc
     2401 ctgctggttt ttgggtcgat tgctatgctg gttagcgcta ccgatggaaa aaaagacaat
     2461 accaacctgg gcgccagcgg agtatatatg ggagcaggat gtgcttttct ggctgccgga
     2521 ctgcctttgt atttctcggc caagaaaaaa atggacacgg catttgatat gtatgttcaa
     2581 tcattgaaaa cagctcaaag caattaccgc atggattttg gcctgacaca aagcggtatt
     2641 gggatgcaaa tgaagtttta atcctgcaac tattttactt ctctcaaaat atgttgaggc
     2701 agccgtaaaa tcaagctgtt tcaacagtct aagttttcat tgcgaatttc ttaattatca
     2761 ttttacactg aaaaaattat aaaaatgacc attttaaatt ttttaaccag cgatgcaccg
     2821 gttttcacct gggttattct tccattattg attttctgtg cccgtatttt cgaccagagc
     2881 gttggcaccc tgcgcatgat atttgttgcc aaaggattta aaaaatgggc gccggtattt
     2941 gcttttttcg aatcgctgat atggttgctg gccataggag aaataatgaa acacctggac
     3001 aatcctttgt gctatattgc ctatgcggga ggctacgcca tggggaatta tgtaggcatg
     3061 ctgctggatg aaaaaatgtc tataggcaat gtcatcatca gggtgatgct caagagcgaa
     3121 gccgccgacc tgattatccg gctgagagaa ttgaactatg gcatcaccat tatgaatgcc
     3181 gaaggtgcta ccggaaaagt gaaagtactt ctttccatca taaaacgcaa agacctcgta
     3241 gaatttattc acgtgctgaa ccaatacaac cctcagacgt tttataccat tgaagaagtg
     3301 cgctcggtaa acgaaggcgt gttcagagcc tcatcaaaaa agcacatctt caggtttcac
     3361 cagatgataa gaaaacacaa atagtaccaa attgaaatat caatcagtcc caaaggacat
     3421 attgatattg ttctataagg gattctacca atgacaacaa taatttattt ggttgtatta
     3481 cagtcatttc ggtcaggcag agccgttgtt ctggacaaaa atgtatttgt tgaaataact
     3541 tatagaacaa cgagtcgaag tcgaccgaaa taaataatcg ataaattctc aaacaatcag
     3601 gtcccatttc cctgcctgcg tgccggacac tccggcaggc agggatacgt cccgaaatgc
     3661 cctatacctt tggccacaat aatttaaatt atcgggacgg ctaccaatga caacaatatt
     3721 gtatttggct gttttacagt tatttcggtc ag
//
LOCUS       DAOSFP010000226         3729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22785_length_3729_cov_3.712030, whole genome shotgun sequence.
ACCESSION   DAOSFP010000226 DAOSFP010000000
VERSION     DAOSFP010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22785_length_3729_cov_3.712030"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLI16_07215"
     CDS             <1..211
                     /locus_tag="PLI16_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOH84386.1"
                     /translation="YQVQGQTTFREEFVTCGGVSLDSIDLNTMQSKVCRNLYFAGEVL
                     DIDGITGGYNFQCAWTTGFIAGKLQ"
     gene            719..2167
                     /locus_tag="PLI16_07220"
     CDS             719..2167
                     /locus_tag="PLI16_07220"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOH84387.1"
                     /translation="MKILNNYYFWIFLLIGLVAPFFVLSFFVFPSADDFSVYNLLQQY
                     SCCEYQEYMYMNWAGRYIANVTDVLNPVQCVTAYRLIPLFLLLLLFGALYVLFRCFFK
                     KFLSTPQLLLFTLVFYALYLNAFPSPAEGIYWYPGAKNYLLANILTLFFLSALYKMMF
                     AAKRTMVFYWWSAALVLAFLTIGLNEISLLLVCGLTLLLLAANGFVHRKLSLPVLLLL
                     VCMALFAVVAVAAPGNFVRMSFFDGSMDVGKSIIQAFVSLLKLLGLYVQNAAFILLSL
                     LAIPFVYSLISAGGIRYRFLINPFLLAFFSLLMLYMLFLPGYLGMSLPPPMRVNALLL
                     LVFLLMWLVNIINLCHYLYLKKTMLPVLPSWFEKALLLCAVLLVLSDFYREPATAVHF
                     RSNIPGAFYDLFVKAPAYKREMLEREKAIAEAKKRGVTDVEVDALKTPAPTIFFVDLK
                     TDSSSWINRNYAAYQGLHSIKLKTSGSAEKTN"
     gene            2170..>3729
                     /locus_tag="PLI16_07225"
     CDS             2170..>3729
                     /locus_tag="PLI16_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84388.1"
                     /translation="MEFLKKTADYLLKNHPEDFGSITVVLPNRRAGIFLKKYLSELIH
                     KPVFLPKIVSIQDFVLGLSAMNMADEITLLVELYKSYAAVKPSEPETFDEFLHWGGKL
                     LQDFDELDQHLAETEFVFSYLKEDKNLALWALDKKPLSEFQKNYLEFYGLLHDIYLDF
                     TARLYERGTGTYGMACRRVAGLLKTSDDVVNSHPAVVFAGINALSASEEFIINCFYQN
                     AKAELLWDGDEYYVKDTLQEAGSFIRRYLSQWDREESKWIDNELLTQPKKITLVAVAG
                     KVGQVKYAAREIASLLAHGVKSEQISVVLNEESLLIPMLNSVPQDIEKFNITMGYPMR
                     FSSVYNFIESVFLLHENAERFRNIKNAKEPLFYHKDLQKLFNHNFVSNIDGGGHLLVS
                     MLKGQERIFYTRDEVNHLLSKCFQTVPGPLAKLFEPWYQQASKAVECLTGFITFIKEE
                     IFSKRGLDAEFLFAAIKIFSRITAVSETAGLTLPANTLHQLFSQHVSQTRVPFVGEPL
                     NGLQIMGMLETR"
BASE COUNT          907 a          767 c          904 g         1151 t
ORIGIN      
        1 ctatcaggtg cagggacaaa ccacgttcag ggaggagttt gttacctgcg gcggtgtgag
       61 tctcgacagc attgatttaa acaccatgca gagcaaggtg tgccgaaatc tttattttgc
      121 cggcgaggtg ctcgatatcg acgggattac cggcggttat aattttcaat gcgcctggac
      181 cacgggtttt attgcaggta agttgcaata aaaactttgg gatggtattc tcaacttagt
      241 gcgcggctca tgggaaacca cttttcatta atatggttgt ttaacaggac cagggttttg
      301 catgattttt taaagctaca cagatccggg cactccggca ggcagagcct ggaatgacag
      361 gctgttggtg tcgttaaagg ggtgtctgaa aaggttataa tgtgattttc atcgccttct
      421 ttacccctcc ctatagggtg gaagtcgctg aaaatcagca ctccctttag ggtggggtaa
      481 tgatgatttt cagcaaggga tagttttgcg acagccccca gattaaaaag aatcaatagt
      541 cattctgaat gcagtaccga tagcttcgtt acgaaatgcg gaatatatat tttttttgtc
      601 ggggttgcct gatctgcttc ccgcaacgtt cacgcacatt cgcaaatttt acatcgttca
      661 tttgtgtata ggacaggtta aattcgatgg aaatgattat ttttgatttt tctaagaaat
      721 gaaaattctg aataactatt atttttggat attcttgctg ataggactgg tagcgccatt
      781 ctttgtcttg tcattctttg tctttccttc agccgatgac tttagtgtgt ataatttgtt
      841 gcaacagtat tcatgttgcg aatatcagga gtatatgtac atgaactggg cagggcgcta
      901 catagccaat gttaccgatg tgctcaatcc tgtgcagtgt gtaacggcat acagacttat
      961 acctttgttt ttactgctgt tgctttttgg agctttgtat gtgttgttcc gttgcttttt
     1021 taaaaaattt ctgagcaccc ctcagttgtt attatttacg ctggtttttt atgcattata
     1081 cctcaatgcc tttccctcgc cggcggaagg gatatactgg tatccgggag caaaaaacta
     1141 tctgctggcc aatatcctga cattgttttt tctttcggct ttatataaaa tgatgtttgc
     1201 agcaaaacgt acaatggttt tctactggtg gtctgccgct ttagtgctgg cctttttgac
     1261 tataggactc aatgaaataa gtttgctgtt ggtctgtggt cttactttgc tgcttttggc
     1321 cgccaatggc tttgtacacc gcaaactgtc cctgccggtt ttgctgttgt tagtctgtat
     1381 ggcattgttt gctgttgtag ctgttgcagc ccccgggaat tttgtccgca tgtctttttt
     1441 tgacggcagc atggatgtgg gaaaaagcat catccaggcc tttgtatcgt tgctaaagct
     1501 gctcggcctc tatgttcaga atgcagcttt tatattgttg tcgctgcttg ccataccttt
     1561 tgtgtactcc ctgatcagtg cgggaggaat acgttaccgt tttctgataa atcctttttt
     1621 gctggcattt ttttcgttgc tgatgcttta tatgcttttt ttgccgggat acctgggtat
     1681 gagcctcccg ccgccaatgc gcgtcaatgc gctgctattg ctggtctttc tgttgatgtg
     1741 gctggtcaac atcataaact tatgtcatta cctgtacctg aaaaaaacga tgttgcctgt
     1801 gctaccgtcc tggtttgaga aggccctgtt gctttgcgct gttttactcg tattgtccga
     1861 cttttacagg gaacccgcaa cagccgtcca cttcaggagc aacatacccg gggcgtttta
     1921 cgatttgttc gtaaaggccc cggcttataa acgggagatg ctggagcgtg aaaaagctat
     1981 agctgaggcc aaaaagcggg gcgtaacgga tgtggaggtg gatgcattga aaacacccgc
     2041 gccaacaata ttttttgtag atttaaaaac tgacagttcc tcgtggatta acaggaatta
     2101 tgcagcgtat caggggttgc attccatcaa gttgaaaacg tctggctctg ctgaaaaaac
     2161 aaattgacta tggaattttt aaaaaagact gctgattatt tgttgaaaaa tcatccggaa
     2221 gatttcggta gtatcaccgt tgttctgccc aacaggaggg cgggtatttt tttaaagaaa
     2281 tacttgtcgg agctgatcca caagccggta tttcttccaa agattgtttc catacaggat
     2341 tttgttcttg ggctttcagc catgaatatg gccgatgaaa tcactttgct ggtagagctc
     2401 tataaaagtt atgcggcggt caagccttcc gaacccgaga cttttgatga gtttctgcat
     2461 tggggaggca aattgttgca ggattttgat gaacttgacc agcatctggc cgaaacggaa
     2521 tttgtttttt cctacctgaa agaggataaa aaccttgcct tgtgggcgct cgacaaaaag
     2581 cccctgagcg aatttcaaaa gaattacctg gaattttatg gtttgttgca cgacatctat
     2641 cttgatttca cggcccgtct ttacgagcgg gggacaggca cttacggcat ggcctgccgc
     2701 agggtagccg gccttctgaa aacttccgac gatgtggtca acagccaccc agccgttgtg
     2761 ttcgccggta tcaacgcact gagcgcttct gaagagttta ttattaactg tttttatcaa
     2821 aatgccaaag ccgaattgct gtgggatggt gatgaatact atgtgaaaga tacgttgcag
     2881 gaagccggga gttttatacg gcgttacctc agccaatggg acagggaaga atcaaaatgg
     2941 attgacaatg agctgttgac gcagccaaag aagatcacat tggttgccgt ggccggaaaa
     3001 gtgggccagg taaaatatgc cgcccgcgaa atagcttccc tgttggcaca tggggttaaa
     3061 agcgagcaga tttcggtggt gctaaacgaa gagagcctgc tgatacctat gctgaattcg
     3121 gtgcctcagg atattgagaa gtttaacatt acgatgggct atcccatgag gttttcttcg
     3181 gtgtataact tcatagagtc ggtgtttctg ttgcatgaaa atgccgaacg tttccgtaat
     3241 atcaaaaatg ccaaagagcc cctgttttat cataaagacc tgcaaaagct gtttaaccac
     3301 aatttcgtaa gtaatattga cggcggtggg catttgcttg tttcgatgct gaaaggccag
     3361 gaacgcattt tttatacccg tgacgaggta aatcatttgt tgtcgaaatg ttttcaaacc
     3421 gttcccgggc cgcttgccaa actttttgaa ccatggtatc agcaggcgtc aaaggcagtg
     3481 gaatgtctta cgggtttcat cacgtttatc aaagaggaaa tcttttccaa acgaggactg
     3541 gacgctgaat ttctttttgc agccataaag atcttctccc gtatcacagc ggtaagcgaa
     3601 acggcaggcc tcacactccc ggccaatacg cttcatcagt tgttttctca gcatgtttcg
     3661 caaaccaggg ttccatttgt gggcgagcct ctcaatggcc tgcagattat gggaatgctt
     3721 gagacacgc
//
LOCUS       DAOSFP010000227         3715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_22901_length_3715_cov_3.260109, whole genome shotgun sequence.
ACCESSION   DAOSFP010000227 DAOSFP010000000
VERSION     DAOSFP010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22901_length_3715_cov_3.260109"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLI16_07230"
     CDS             complement(<1..284)
                     /locus_tag="PLI16_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84389.1"
                     /translation="MRKLLFTGLCLFCLNNVFAQKVLILENLSLGKSYKFFTGDEIGI
                     RINDTTPKVSGKITEILDSSIIIANHTACDLKEIAVVYKGRRGIQIVSAS"
     gene            complement(288..641)
                     /locus_tag="PLI16_07235"
     CDS             complement(288..641)
                     /locus_tag="PLI16_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOH84390.1"
                     /translation="MNIEDVREYCLSKKGVTESFPFDATTLVFKVLGRIFLLTDLESE
                     FSINIKCNPDLAVELRERYDCVEPGYHMNKTCWNTVLIDGDIPDSELYRWIDHSYEEV
                     VKKLTKKQKELLDQM"
     gene            864..2414
                     /locus_tag="PLI16_07240"
     CDS             864..2414
                     /locus_tag="PLI16_07240"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOH84391.1"
                     /translation="MTTLRQRTEELRRRMRGASRGGGEAAIEKQKATGKMTARERILA
                     VLDSGSFHEYDLFVEHAGKDFDMDKKYFPGDGVVTGTGTINGFPICIYAQDFTVAGGS
                     LGYMHAKKITKIMDHAMKLKVPCIGINDSGGARIQEGVNSLAGYGEIFFRNTQASGVI
                     PQISVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKTVLGEEISMEDLGGAA
                     VHSEITGNAHFFATSENECFDQIKKLMSFIPWNNTKKADPFPIKKPKNKVFKLTEIFP
                     TDSKKPYDVRLVIKAICDDSDFFEVHERFAANIVVGFARMNGQSVGFVANQPMVLAGV
                     LDVDSSDKAARFIRFCDAFNIPLVTLVDLPGYLPGIDQEHAGVIRHGAKVLYAYSEAT
                     VPKITVILRKAYGGGYIAMCSNHLKADFVLAWPTAEIAVMGPEGAANIIFRNEIQNAP
                     NPEEARKKKIEEYKEKFANPYVAAAFGYVDAVIEPLKTREFILHALEISAQKKEDRPV
                     KKHGIPPF"
     gene            2424..2753
                     /locus_tag="PLI16_07245"
     CDS             2424..2753
                     /locus_tag="PLI16_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOH84392.1"
                     /translation="MKNSKKSEPNFRKLTVLGEEYTTLYTRKYENRKPHKPADHKKLY
                     SFIPGKIKDVFVEKGVAVSPGDKLLILEAMKMDNEIIAPVAGTVKKVYVVSGQMVAKN
                     ELLIEIS"
     gene            complement(2793..3599)
                     /locus_tag="PLI16_07250"
     CDS             complement(2793..3599)
                     /locus_tag="PLI16_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84393.1"
                     /translation="MKKVVCLFSMIAVFFISESYAQKEKLVRISTVETSGSYLAADNL
                     GYIYLINESTLKKFDKDGLLQYTYTEFSDGKISQMDVNDPLKIMILNADFGKIKYLDN
                     KLTLKKDFILLSDLGYPNAQLVAGSYDNGFWLFDPLTNQVVRFDKYLRASQNSGNIAD
                     ITGIEVNPVFMTEADNVLYLNDPQQGIFLFDRYGTYLRQVPLKNLKTFQVFPPRVIFS
                     EGRKLKMFDLKTFEESALDIPCDEEPLQTLWNNERVYVLTEKTLLIFRME"
BASE COUNT         1107 a          818 c          762 g         1028 t
ORIGIN      
        1 aacgacgcag aaacaatctg aattcctcgc cttcctttat aaacaactgc gatctctttc
       61 aaatcacagg cagtatggtt tgcaataatt attgaagaat ccagaatctc cgtgattttt
      121 cccgacacct tgggagtcgt gtcatttatt ctgataccga tttcatctcc ggtaaaaaat
      181 ttatatgact ttccaaggct taaattttca agtataagaa ctttctgtgc aaaaacattg
      241 ttcaggcaaa acaaacataa tccggtaaaa agtaattttc tcattcatta catctgatcc
      301 aataattcct tttgcttttt tgtcagcttc tttaccactt cctcatatga atggtcaatc
      361 cagcgataca gttcgctgtc gggtatatcg ccatcgatta ataccgtgtt ccagcaagtt
      421 ttattcatat gataaccggg ttcaacgcag tcatatcgtt cacgcaattc aacagccaga
      481 tcgggattgc atttgatatt gatgctgaat tcgctttcca ggtcggtcaa caaaaaaatt
      541 ctccccagca ccttaaaaac caaagttgtt gcatcaaaag gaaacgactc cgttacacct
      601 ttttttgaca aacaatattc cctgacatcc tcgatattca taattttttt acaatcagat
      661 tatttcagaa caaatgtaag ttatacgatt aatatttgca acaaaaagtc cgcatttagg
      721 tatttaaaga tattattttt aaataaaaca cattattcct ttaacattgg ttaacaaaaa
      781 agtcgcccaa ggctcctaaa aaaaagaata tctttgtgag ttttaataag gattttctaa
      841 aaataacaag gatattcaac gacatgacga cattacggca aagaacagaa gaactccggc
      901 gcaggatgcg tggcgcctca cggggcggcg gagaagcagc cattgaaaaa caaaaagcca
      961 ccggaaaaat gactgcccgg gagcggattc tggcagtact tgattcggga tcttttcatg
     1021 agtacgacct ctttgtggaa catgcgggga aagattttga catggacaag aaatattttc
     1081 ccggcgatgg tgtagttacc ggaacaggaa ccatcaacgg attcccgata tgtatctatg
     1141 cccaggattt tactgttgcc ggcggttcgc tgggctatat gcacgccaaa aaaattacga
     1201 aaatcatgga tcacgccatg aagcttaaag tgccctgcat aggtatcaac gacagcggcg
     1261 gagcgcgtat ccaggaaggt gtgaattcgc tggccggtta tggcgaaatt tttttccgaa
     1321 atacgcaggc ttcgggtgtc attccgcaaa tttccgtcat cctggggccc tgtgccggcg
     1381 gggcggttta ttctcctgcg ctcactgact ttgtttttgt ggtggacaaa atttcaaaaa
     1441 tgtttatcac aggccccgaa gtcatcaaaa cggtactggg cgaagaaatt tcgatggaag
     1501 acctgggcgg ggcagctgta cactctgaaa ttaccggcaa cgctcatttt tttgccacca
     1561 gcgaaaacga atgtttcgac cagataaaaa aacttatgtc attcattccc tggaacaaca
     1621 ctaaaaaagc cgatcctttc ccgataaaaa aacctaaaaa taaagtcttt aaactgactg
     1681 aaattttccc tactgactca aagaaacctt acgatgtgcg ccttgtcatt aaagctattt
     1741 gtgatgactc tgactttttt gaagttcacg aacgttttgc agcaaacatc gtggtaggtt
     1801 ttgccaggat gaatggacaa tcggtaggtt ttgtcgccaa ccagcctatg gtgcttgccg
     1861 gagtgcttga tgtggactct tcggataaag cagcccgttt tatccgtttc tgtgatgcat
     1921 ttaatatccc gctggtaacc ctggttgacc tgccgggcta tcttccgggc atcgaccagg
     1981 aacacgccgg cgtgatacgc cacggcgcaa aagtacttta cgcttacagc gaagccaccg
     2041 tccccaagat tacggtgata ctccgcaagg cttacggcgg aggatatatc gccatgtgtt
     2101 caaaccacct gaaagccgac tttgtgctgg catggcctac ggctgaaata gccgtcatgg
     2161 ggcccgaagg agccgccaat attattttcc gtaacgaaat tcaaaacgct ccgaatcccg
     2221 aagaggcacg aaagaaaaaa attgaagaat ataaggaaaa attcgccaac ccctatgttg
     2281 cagcggcctt cggatatgtt gatgccgtta ttgaaccgct caagaccagg gaatttatac
     2341 tgcatgccct ggaaatatcg gcccagaaaa aagaagaccg gccggtaaaa aaacacggta
     2401 tcccaccatt ctaatccatt gagatgaaaa attcaaagaa aagcgagccc aattttcgga
     2461 aacttaccgt tctgggagaa gaatatacca ccttatacac ccggaaatat gaaaatcgca
     2521 aaccacataa acccgccgac cacaaaaaat tgtactcttt tatccccgga aaaattaaag
     2581 atgtttttgt ggaaaaagga gttgcagtta gccccggcga taaactactg atactggaag
     2641 ccatgaaaat ggataatgaa atcattgctc cggtggccgg taccgttaaa aaggtttatg
     2701 ttgtttcagg gcaaatggta gctaagaatg agttactgat agaaatctcc tgaagtttct
     2761 gtcccatgtt attttctaag ttggccactg acttattcca tccggaaaat aagtaatgtc
     2821 ttctccgtaa gcacataaac ccgttcatta ttccaaaggg tttgcagggg ctcttcatca
     2881 caggggatgt ccaaggcaga ttcttcaaaa gttttcagat cgaacatttt cagtttacga
     2941 ccttccgaaa aaataacacg gggtggaaac acctgaaagg tcttgagatt ttttaagggc
     3001 acctgccgca gataagtacc gtagcggtcg aacaggaaaa taccctgttg cgggtcattc
     3061 aggtagagca cattgtcggc ctctgtcata aatacagggt ttacttcaat ccccgtaata
     3121 tcggcaatat tgccactgtt ttggctggct cttaaatact tgtcgaagcg gactacctga
     3181 ttggtcaacg ggtcgaaaag ccagaaaccg ttatcgtacg atcccgcaac cagttgggca
     3241 ttgggatatc caagatcgga caacaaaatg aaatcttttt tcagggtaag tttattgtcg
     3301 aggtatttta ttttcccaaa atcagcattc agaatcatga tcttcaaggg atcgtttaca
     3361 tccatctggg aaattttccc gtcactaaat tcggtgtaag tatattgcaa caagccatcc
     3421 ttgtcgaatt tcttcagcgt actttcatta atcaggtata tatagccaag gttgtcggcg
     3481 gcgaggtagc tcccgcttgt ttccacggtt gaaattcgta ctaatttttc tttctgggca
     3541 taactttcac ttataaaaaa cacggctatc attgaaaaca aacaaacaac ctttttcatt
     3601 ttttgatcag tttcaatatt tccgaaacat aagtacgttc attggcaagc ctggggactt
     3661 tgtgttgtcc gcccaattta tttttacttt tcagccattg ataaaaagtg ccacc
//
LOCUS       DAOSFP010000228         3678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23219_length_3678_cov_3.274358, whole genome shotgun sequence.
ACCESSION   DAOSFP010000228 DAOSFP010000000
VERSION     DAOSFP010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23219_length_3678_cov_3.274358"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1064)
                     /gene="gmd"
                     /locus_tag="PLI16_07255"
     CDS             complement(27..1064)
                     /gene="gmd"
                     /locus_tag="PLI16_07255"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOH84394.1"
                     /translation="MKTALISGITGQDGSYLAELLLEKDYTIHGIIRRSSSFNTFRID
                     HLYNNPNILNKRLFLHYGDMNDSSNLNRIIEKTQPDEIYNLAAQSHVQVSFEVPEYTA
                     EADAIGALRFLDAIKDVGIKSKFYQASTSELFGLAQAVPQNEKTPFYPRSPYAVAKLY
                     AYWIVVNYREAYNLFACNGILFNHESERRGKTFVSRKISVAVANIMAGKDGMLLLGNL
                     DSRRDWGYAPEYVEGMWRILQHHKPEDFVLATNETHTVREFVEEAFKVLDEEIEWRGK
                     GLEEKGYLKSSGKPVVGIDTRYFRPTEVDLLIGDYSKAKNLLRWAPKTTFKDLVKIMV
                     SADFEKAKKRM"
     gene            1361..>3678
                     /locus_tag="PLI16_07260"
     CDS             1361..>3678
                     /locus_tag="PLI16_07260"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH84395.1"
                     /translation="MRNLKIIGLFLLLFFSMSGTNIAQILTPDQIPGLSLWLKADAGP
                     LLNGSNVFQWNDYSGNSNNAVQSIVESQPILVSNALNGLPVVRFDGANDFITGGVIPN
                     IDTSSISVFVVAYGASGASANLYGMFNINAYTNGFCLDRHGWNEILSLYNNGSIIPSP
                     DGTLPNAGYNFKIFEAVKKYNIETELFINGISQASSSNSLLNGPFTNADYVIGYSQGW
                     AYLNGDIAEILVFKQSLSAVDRQSVENYLRMKYARQIDLGPDIIAYGFQPLTLNAQSG
                     FTNYLWNDGSTDSTLSIHSSGAYWVQANDAFGYVTSDTVHVAYPNTRLNYQNDTAICL
                     GSDLLLRSLIPDKSNYTFLWSNGSTDTVINVSAGGSYWVKITDTLGYFAYSDTVQVAI
                     DSFALHVNLGNDTSFCSGNFLGIQPPLGNYGSPVYTWPDGSHDTTLVVTASGSYHVSV
                     TNQSGCTGTDTVHVTITGTAPTTLFDADTVCFGDNTVFTDLSTVQSGDTLVTWRWQFT
                     ANDTSNLQNPIFAFPAYGIHSVTLTAYTTAGCAKSYTKNVLVYPVPVPAFSADISCMN
                     NPLQFADESSVMPGDAITTWDWDFGDSSPHSNQQHPVHVYMQAGNYSATLTVQTQNGC
                     QRSVTDSIEVVSFSALPLVPQILSPANNAIVSDTSINFSWAPSQNSVRYKIVLASDAT
                     FNNLLVDSIVYNPDFQRTFSAANQVYYWKVIAYNICSDSVVTQQHEFEIFYPGEISGM
                     ALWLKADAGPLLNGTNVFQWNDYSGNSNNAVQ"
BASE COUNT          977 a          804 c          791 g         1106 t
ORIGIN      
        1 catagatttt gctgcttttt tccatattac atcctttttt ttgctttttc aaaatctgcc
       61 gacaccataa tctttacaag gtctttaaag gtggttttag gcgcccatct caggagattt
      121 tttgcttttg aataatcgcc tatcagcaga tctacttcgg taggcctgaa atagcgggta
      181 tcaatgccca caacaggttt gcccgacgat ttcaggtatc ctttttcttc cagtcctttt
      241 cccctccatt ctatttcttc gtccaaaact ttaaaggctt cttccacaaa ctccctcacg
      301 gtatgagttt cgttggtggc cagcacaaaa tcttcgggtt tgtggtgttg caatatccgc
      361 cacatgcctt ccacatattc tggtgcatag ccccagtcgc gccgggaatc gaggttgccc
      421 agcaacaaca taccgtcttt gccggccata atgttggcca cagccacact tatttttcgg
      481 gaaacaaagg ttttgccgcg ccgttcgctt tcgtggttga agagaatacc gttgcaggca
      541 aaaagattat atgcctcgcg gtagttgacc actatccagt aggcatataa tttggccacg
      601 gcataagggc tgcggggata aaaaggtgtt ttttcgtttt gcggcacggc ttgtgccagg
      661 ccaaaaagtt cggaggtaga ggcctgataa aattttgatt ttattcccac atccttgatg
      721 gcatccagaa accgcaatgc ccctatggca tcggcttcag cggtatattc gggcacttca
      781 aaagatacct gcacatggct ttgtgccgcc aggttataaa tttcatcggg ctgtgttttt
      841 tcgataatac ggttcaggtt ggacgagtcg ttcatgtcac cgtaatgtaa gaacaagcgt
      901 ttgttcagta tgttgggatt attgtataaa tggtctatcc tgaaggtgtt gaatgagctg
      961 gatcgcctga taatgccgtg aatagtatag tctttttcca ataataattc ggcaagataa
     1021 ctgccgtcct gtcctgtaat ccctgaaatt agcgcggttt tcatattgtt gttttcaaat
     1081 gtaaaagatc atttattaca aatatccggt gttgattttg cctaagagcc ttctcctgtg
     1141 gtcaaataaa gcacattcgg ataaagtggc agaatcatct gtttgccgga ttcaatattt
     1201 tgggtaaaag tacaaagaaa aaatttatct ccgtaatatt cggttgagat tggacttctt
     1261 aatggaatgc cctgccggaa ttaaaaaaat aaaatgtcaa tattaatttt ttttggtctt
     1321 tcatctcatt ctgctatatt tgtataaaaa tcacctcaat atgcgaaatc tgaaaattat
     1381 aggattattt cttttattat ttttttcaat gtcaggaact aatattgcac aaatattaac
     1441 gcccgaccaa atacccggct tgtcattatg gttgaaagct gatgcggggc ctctactcaa
     1501 tggctccaat gtttttcagt ggaacgacta tagcgggaac agcaataatg ccgtacaaag
     1561 catcgttgaa tctcagccca tactggtgtc aaatgcgttg aacggattgc ctgtagtgag
     1621 gtttgacggg gcgaatgact ttattacggg aggtgtgata ccgaatattg atacatcgtc
     1681 aatcagcgtt tttgtagttg cctatggagc atcaggcgcc agtgcaaatt tatatgggat
     1741 gtttaacatc aacgcttata ccaatggctt ttgtcttgac aggcatggat ggaacgaaat
     1801 actatcatta tataacaatg ggagtattat tccttctccc gatggaacac tacccaatgc
     1861 cggatacaat ttcaaaattt ttgaagcggt aaaaaaatat aatattgaaa cggaattatt
     1921 tattaatgga atttcacagg ccagttcatc caattcctta ttgaacgggc cgtttaccaa
     1981 tgctgattat gtgataggat attcacaggg ttgggcgtat ttaaacggag acatcgcaga
     2041 aatactggta tttaaacagt cgctaagcgc tgttgatcgt caatctgttg aaaactatct
     2101 caggatgaaa tatgcccggc aaattgatct gggtcctgat atcattgctt atggttttca
     2161 gccgttaacc ttaaatgcac aaagtggttt tacgaattat ctctggaatg acggcagtac
     2221 agattcaacc ttaagcattc actcaagcgg ggcttattgg gtacaggcca acgatgcttt
     2281 cggatatgta acttcagata ccgtccatgt agcttatccc aacacccggc tgaactatca
     2341 gaacgatacc gccatatgtt tggggtctga tttattatta cggtcattga taccggataa
     2401 aagtaattat acatttctct ggtctaacgg gtcaacggac accgtaatca acgtcagcgc
     2461 cggcggcagt tactgggtaa agatcaccga caccctgggc tattttgctt attccgatac
     2521 cgtacaagtt gcgatagact catttgccct gcatgtcaat ttggggaatg atacttcttt
     2581 ttgttcagga aattttctgg ggatacaacc gcccttgggt aattacggaa gtccggttta
     2641 tacgtggccg gacggttcgc acgacaccac tctggttgtt acggcaagcg gaagttatca
     2701 tgtaagcgtt accaaccagt cgggatgcac cggtacggac acagtacatg ttaccataac
     2761 agggacagca cccaccacct tatttgatgc cgacactgtt tgttttgggg acaatactgt
     2821 ttttaccgac ctgtctactg tccaatcggg agacaccctt gtaacatggc gctggcagtt
     2881 tacggcaaat gacacatcga atcttcagaa cccaatattt gcatttcccg catacggcat
     2941 acacagcgta acacttacgg cttatactac ggcgggctgt gcaaaatcat acacaaaaaa
     3001 tgtattggtt tatcccgttc ctgttccggc atttagcgcg gatatatcct gcatgaacaa
     3061 tcccctgcag tttgccgatg agtcttctgt gatgccggga gatgccatta caacatggga
     3121 ttgggacttt ggtgactctt ctccacattc caatcagcaa caccctgtac atgtttatat
     3181 gcaggcaggg aattactctg ccacgcttac tgttcagaca caaaatggct gtcagcggtc
     3241 tgttacggac agcatagagg ttgtttcctt ttccgccctg ccattggttc cccagatact
     3301 ttccccagcc aacaacgcca tcgtcagcga taccagtatt aactttagtt gggcgccttc
     3361 acagaacagc gtacggtata aaatagtcct tgcctcagat gccacattta acaaccttct
     3421 tgtagacagc atcgtatata atcccgattt tcagagaaca ttcagcgctg ccaaccaggt
     3481 ctattactgg aaggtcattg cgtataatat ttgttcagac tctgtggtaa cacaacagca
     3541 tgagtttgag attttttatc cgggcgagat atctggcatg gccttatggc tgaaagctga
     3601 tgctgggcct ttactcaatg gtaccaatgt ttttcagtgg aacgactata gcgggaacag
     3661 caataatgcc gtacaaag
//
LOCUS       DAOSFP010000229         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23538_length_3641_cov_3.862242, whole genome shotgun sequence.
ACCESSION   DAOSFP010000229 DAOSFP010000000
VERSION     DAOSFP010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23538_length_3641_cov_3.862242"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLI16_07265"
     CDS             <1..746
                     /locus_tag="PLI16_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH84396.1"
                     /translation="KNVLTGSGLPGKLADCSEKDASLCELFLVEGDSAGGTAKQGRDR
                     KIQAILPLRGKILNVEKAMPYKVFESEEIKNMFTALGITIGTADDSKALNLDKLRYHK
                     IIIMTDADVDGSHIATLILTFFFRYMHELIERGHVYIATPPLYLIKKGNDARYCWTEE
                     QREQVVAEFSQTTKESNIHIQRYKGLGEMNAEQLWDTTMNPEKRTLRQVNIVNGTDAD
                     RIFSMLMGDEVPPRREFIEKNAKYARIDA"
     gene            783..1469
                     /locus_tag="PLI16_07270"
     CDS             783..1469
                     /locus_tag="PLI16_07270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH84397.1"
                     /translation="MNVIITGAGKGLGFELCKEFLRHADTYVLAISRDIGNLRPLTSD
                     YHSMQALAFDLEEDDYTQLVDHVYLLPGHKIDILINNAGLLIKKAFKDLNTEDNRRML
                     EVNYLAPYKLIQKLLPCFSDNAHIVNISSMGGFQGSEKFKGLAGYSASKAALACLTEC
                     LAGELKANNIKANCLCLGSVQTSMLEQAFPGYQASVTAGEMAVFISNFAINAHKFING
                     KIIPVALTNP"
     gene            1629..2420
                     /locus_tag="PLI16_07275"
     CDS             1629..2420
                     /locus_tag="PLI16_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84398.1"
                     /translation="MKKFLLYGLMLLIIPVCGFSQSKTSITIKSMTIDNGDTTVSERT
                     YNSDDDNIILNDSIFGENNPFIFFNRDYSLDTNFDDNFDRIIRNEMQDFFQHFNTIPF
                     DNPGQDNEFFSRPFGLDFDSIFSSENPVQMLPDTNNQYGQKENSRPYRPIKRNSITVE
                     NIIVPDRQTVEGYSITPGDDEGIVRIVFQLDPGKATQLIIKNDRKKQIYKEKIPVSKG
                     VYTRLFDFSTYEAGTYYLELHQGKKISASTITIPEGNKNNFQFIF"
     gene            2545..2622
                     /locus_tag="PLI16_07280"
     tRNA            2545..2622
                     /locus_tag="PLI16_07280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2580..2582,aa:Val,seq:gac)
     gene            2933..>3641
                     /locus_tag="PLI16_07285"
     CDS             2933..>3641
                     /locus_tag="PLI16_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84399.1"
                     /translation="MKKITGFILLLLIAGNACAQWSSDTLRRASGFYIAPAITGMYNK
                     NQTQTPQPAFGLNLGYRFINKLKYGFFLEGGVGLSWLGANYSVEEITVNTMGRNWTYT
                     EDKRANQLYISTPFLAGYKTPKGKVRFQAALGVAFNIKYSDFQRVTIDGDYPFGLKGT
                     KISGEEASFGTSFSGIVKAGISIPLTDRMNLDILPGLRYNFASTMPEDLDFRTCVTTR
                     HQNWSAGIDIGLVWALDN"
BASE COUNT         1112 a          777 c          761 g          991 t
ORIGIN      
        1 gtaaaaatgt gctcacaggt tcgggtcttc cgggaaaact ggccgactgc agcgaaaaag
       61 atgcttcgtt gtgtgaactt ttcctcgttg agggagattc cgccggtgga acagccaaac
      121 agggacgcga cagaaaaatt caggctattc tgcccctcag gggaaagatt ttgaatgtgg
      181 aaaaagcaat gccttataaa gtctttgaat cggaagaaat aaaaaatatg tttaccgccc
      241 tcggtattac cattggcacg gctgatgact ccaaagcgct taacctcgac aaacttcgtt
      301 accacaaaat catcatcatg actgatgccg atgtggacgg aagccacatc gccaccctga
      361 tccttacctt ctttttccgc tatatgcacg aacttatcga acgcggtcat gtctatatag
      421 ccacaccgcc cctgtatctt atcaaaaaag ggaatgatgc ccgctattgc tggacagaag
      481 aacaacgcga gcaggtggtg gccgaatttt cgcagactac taaagaatca aacatccaca
      541 tacaaagata taaaggtctt ggtgaaatga atgccgaaca gttgtgggat acgactatga
      601 accccgaaaa acgcaccctg cggcaggtaa acattgtcaa tggcaccgat gccgaccgta
      661 ttttctccat gctgatgggc gatgaagtac ctcccagaag agaatttatt gagaaaaatg
      721 ccaaatatgc cagaattgac gcctgatggc tcttttgggc aagcggcata aaaattcagg
      781 acatgaatgt cattataaca ggcgcaggaa aaggattggg ttttgaactt tgcaaagaat
      841 tcctgcgcca tgctgatact tatgtgctgg ccatttcgcg cgatattggc aatctacggc
      901 cccttaccag cgactaccat agcatgcagg cacttgcctt cgaccttgaa gaagatgact
      961 acacacaact tgttgaccac gtatatttat tgcccgggca taagattgac atattgatca
     1021 acaatgctgg attattaata aaaaaggcat ttaaagacct caataccgaa gataaccggc
     1081 gaatgttgga agttaattac ctggcgcctt acaaacttat tcaaaaactc cttccctgct
     1141 tttctgataa tgcgcacatt gtaaacatca gcagcatggg tggctttcag ggcagcgaaa
     1201 aattcaaggg gctggcagga tatagcgctt caaaagccgc cctggcctgt ctcaccgaat
     1261 gccttgccgg cgagctaaaa gcaaacaata tcaaggccaa ttgtttgtgt ttgggttcgg
     1321 tgcagacaag catgctggaa caggcatttc cgggatatca ggcttcagtt acagccggcg
     1381 aaatggccgt ttttatcagt aattttgcta ttaacgcgca taaattcatc aatggtaaaa
     1441 taatccctgt tgcattgact aatccatgat tttttcaaca aatacaattt tttatttttg
     1501 tattcgtttt ttcaaagaaa attacaaaaa cattttggct gaaagcctga tgttggttgg
     1561 aaaaagtcct cacagtacca gcaatggagg cagattagcc aaaagaatca acttaaaatt
     1621 ttacacctat gaaaaaattt ttgctatacg gcttaatgct tctgatcatc ccggtttgcg
     1681 ggttttcaca atcaaaaacc agtattacta tcaagtcgat gactattgac aatggcgata
     1741 caacggtgag tgaacgtacc tataattctg atgacgataa tattatttta aacgacagta
     1801 tttttggtga gaacaacccg tttatttttt tcaacaggga ttattcttta gataccaact
     1861 ttgatgataa ctttgacagg attatacgga acgagatgca agattttttt cagcatttta
     1921 ataccattcc atttgataat cccgggcagg ataatgaatt tttcagcagg cctttcggtt
     1981 tagatttcga ctcgattttc tccagcgaaa accctgtaca gatgcttccc gacactaaca
     2041 atcagtatgg tcaaaaagag aattcacgac cttacaggcc catcaaacga aatagtatca
     2101 ctgtggaaaa tattatagtg cccgaccggc agacggtgga gggctattcc ataacacccg
     2161 gcgatgacga aggcattgtt aggatagttt ttcaactcga ccccggaaaa gccacgcagt
     2221 taatcataaa aaacgacagg aagaaacaaa tttataaaga gaagattcct gtatcaaaag
     2281 gcgtttacac ccggctgttt gattttagca catatgaagc cggcacatat tacctagagc
     2341 tgcatcaggg aaagaaaata tcagccagca ccattacgat tccagaaggc aataaaaata
     2401 attttcagtt tattttttaa aaaatattac ttttgcacct caagtcagta gttcgctttt
     2461 gattcagaat ataattcttt ttgaaaaacc ctggtgaaat aagcgaaaga aaaaaacgtt
     2521 ctttttgttg tcatgaattc ataagtgcct ttagctcagc tggtttagag cattaccttg
     2581 acagggtagg ggtcactggt tcgaatccag taaggcacac caattaaata aaggtttcag
     2641 agtgtctttg aaaccttttt tttacagctt gaaagttgca agcacgcttc ttccccaccc
     2701 cggcccttct ggttgataga taataccttt attacacatt aagcattctc attgccggcc
     2761 ccaagggtga aaaattcgta tgaattattc aggttaaaga tttgcaggaa gacgcgcaaa
     2821 aatttttgag acaggactat aatatttcat ggaattgtac cagtcatttt tatttcatta
     2881 aaacagcaac aaaattatta tgtaagtttg ttcccgatta aaattccata caatgaaaaa
     2941 aatcaccgga tttattctat tattgttgat agcgggcaat gcctgtgcac aatggtcttc
     3001 tgatacgttg cggcgcgctt caggtttcta tattgctccg gccataacgg gtatgtataa
     3061 caaaaaccaa acccagacac ctcaacccgc ctttgggctg aacctgggtt atcgttttat
     3121 caacaaactc aagtacggat ttttcctgga aggcggagtg ggcctctcct ggcttggcgc
     3181 caattattct gtagaagaga tcactgtcaa tactatgggc cgtaactgga catataccga
     3241 agataaaaga gcaaaccagt tatatatcag tacgcccttc cttgccggct acaaaacacc
     3301 gaaaggaaaa gtgcgttttc aggccgcgtt aggtgtagct tttaatatta agtacagcga
     3361 ctttcaaagg gtaaccattg atggcgacta tccttttgga ctaaaaggaa ccaaaatctc
     3421 cggagaggaa gcttcttttg gaaccagttt cagcggtatt gtgaaggccg gaatcagcat
     3481 cccgctcacc gaccgtatga accttgatat attgccaggc ctgcgttaca actttgcatc
     3541 aaccatgccg gaagatttgg atttcaggac ctgtgttacc acacggcacc aaaactggtc
     3601 ggcaggcatc gacatcgggc tggtgtgggc gctcgacaat a
//
LOCUS       DAOSFP010000230         3638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23563_length_3638_cov_3.851242, whole genome shotgun sequence.
ACCESSION   DAOSFP010000230 DAOSFP010000000
VERSION     DAOSFP010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23563_length_3638_cov_3.851242"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLI16_07290"
     CDS             <1..274
                     /locus_tag="PLI16_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84400.1"
                     /translation="PDKPLIEIATQNMLKGISIVISDNGIGISKANQKKIFEKLYRVS
                     TGDVHNVKGFGLGLHYVKTIVEAHGGHVDVESEIKKGTKIKIYLPF"
     gene            294..1004
                     /locus_tag="PLI16_07295"
     CDS             294..1004
                     /locus_tag="PLI16_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016268617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH84401.1"
                     /translation="MTIKNINVLLAEDDKNFGEMLKTYLTAKGYNTKLCINGEEALNE
                     FLANPYDCCIFDVMMPVKDGFTLAKEVRKKDKKIPILFLTARSLSDDILKGFQIGADD
                     YITKPFNMEVLLMRMNAILRRAGGGAKTGNAGNTYRLGDYVFDHNRQVLTILGKEQKL
                     TSKESELLKLLCENANDLLDRSIALKEIWHDDSYFNARSMDVYITKLRKYLREDDSIE
                     LINVHGTGFKLVGNIENI"
     gene            1062..2486
                     /gene="ahcY"
                     /locus_tag="PLI16_07300"
     CDS             1062..2486
                     /gene="ahcY"
                     /locus_tag="PLI16_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOH84402.1"
                     /translation="METMTNTQIPYKVADLKLADWGRKEIEIAEKEMPGLMALREKYG
                     QIKPLKGVRISGSLHMTIQTAVLIETLVELGANVRWASCNIFSTQDHAAAAIAEAGVP
                     VFAWKGESLEEYWWCTLQALSFPDGKGPQLVVDDGGDATLLIHKGFQAENDAKVLNVV
                     ATSHEEEVILNLLKKVLVEEPGKWHNLVSEWKGVSEETTTGVHRLYQMMEKGELLVPA
                     INVNDSVTKSKFDNLYGCRESLADGIKRATDVMIAGKVVVVLGYGDVGKGCAKSMRSY
                     GARVIVTEIDPICALQAAMEGFEVTCTEDALAEGNIFVTTTGNRDVITVEHMAKMNDQ
                     AIVCNIGHFDNEIQVSKLEKFPGIKKITVKPQVDKYIFPDGKAIFLLAEGRLVNLGCA
                     TGHPSFVMSNSFTNQTLAQMDLWKNKDVYKIGVYRLPKYLDEEVARLHLEKIGVKLTK
                     LSKEQADYIGVPVEGPYKADHYRY"
     gene            2544..>3638
                     /locus_tag="PLI16_07305"
     CDS             2544..>3638
                     /locus_tag="PLI16_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOH84403.1"
                     /translation="MAKKLYRFVEYLFFVAFVSLAFSCANIVSPTGGPKDITPPKVKT
                     TEPPNYSAGFTGNKIRITFNEYVVLEDQANQVVVSPPVEKEVEFNISGKSIIIDLPDN
                     IRDSITYTVNFGTSIKDLNEGNPLRNYEFVFSKGSAVDSMSIQGRVYDALLRDPQNGV
                     LVMLYDAGDDSLPIKSRPLYLAKTNAEGNFVIGNIKKQKYKIFALRDMNADMLFNQPT
                     ESIAFADSTLTPQYIVKKTDTAKIKADSSSIAQDSLMPVEPVRNTILYLFTQKDTSQK
                     LLKARSDEYRQCKFVFKNPVKDLQIIVTNRQLPENWRVDEFSAGKDTLVCWLKDPDMD
                     TLKMIFMDKGIVFDTAELALKPKPSDQQAVK"
BASE COUNT         1129 a          758 c          827 g          924 t
ORIGIN      
        1 acctgacaaa ccgcttatcg aaattgctac acaaaatatg ctgaaaggga taagtatcgt
       61 tatttccgat aacggtatcg gtatttccaa ggccaatcaa aaaaagatat tcgaaaagct
      121 atacagggtt tccacaggtg atgtgcataa tgtaaaaggc tttggactgg gtttgcacta
      181 tgtaaaaacc attgtggaag ctcatggcgg tcatgtggat gtagaaagcg aaataaaaaa
      241 agggacaaaa ataaagatat atttgccgtt ttaattcaat taaaaattct attatgacca
      301 taaaaaatat caatgtgctt ttggcggaag acgataagaa ttttggtgaa atgcttaaaa
      361 cttatcttac cgccaaggga tataacacca aattgtgcat aaatggcgaa gaggctctga
      421 acgaatttct ggctaatcct tatgactgct gtatttttga tgttatgatg cccgtcaaag
      481 atggctttac cctggcaaaa gaagtgcgga aaaaagataa aaaaataccc attttgtttc
      541 ttacagcccg ttccttgtcg gatgatatac tgaaagggtt tcagatagga gccgacgact
      601 atatcaccaa gcccttcaat atggaggtgt tgcttatgcg tatgaacgcc atactgcgaa
      661 gggccggcgg tggcgctaaa acgggtaatg caggcaacac ttatcgtctg ggagattatg
      721 tctttgatca caacaggcag gtattgacta ttttgggaaa agagcaaaaa ctaacctcaa
      781 aagaatcgga attgctgaaa ctcttgtgcg aaaatgccaa cgacctgctc gaccgaagta
      841 tagccctcaa ggaaatatgg cacgacgaca gctatttcaa tgcccggagc atggacgtat
      901 atatcaccaa actcagaaaa tatcttagag aagacgatag tatcgagctt atcaatgttc
      961 atggcaccgg ttttaagctg gtaggcaata tcgaaaacat ctgaaaatat attttttcaa
     1021 ttcccgcaaa atgtttagtt ttgcaccctc aaaacattaa aatggaaaca atgacaaata
     1081 cacagatccc ttacaaagta gctgatttaa aacttgccga ctggggaaga aaagaaattg
     1141 aaatagccga aaaagaaatg ccgggactca tggccctgcg tgaaaaatac ggacaaataa
     1201 aaccgctgaa aggcgtcaga atatccggct cgctgcacat gaccatacaa accgccgtgt
     1261 tgattgaaac cctggtggaa cttggtgcca atgtgcgttg ggccagttgt aatatttttt
     1321 cgacacagga ccatgctgca gcggctatcg ctgaagcagg cgtgcctgtt tttgcctgga
     1381 aaggcgaaag cctcgaagaa tactggtggt gcaccctgca ggcattatcg tttcctgatg
     1441 gcaaaggccc gcagttggtg gtggatgatg gtggcgacgc taccctgctt atccataaag
     1501 gatttcaggc cgaaaacgat gccaaggtcc tgaatgtcgt agctacaagc cacgaagaag
     1561 aggtgatcct gaacctgctc aaaaaagtac tcgtcgaaga acccggtaaa tggcacaatt
     1621 tagttagcga atggaaaggt gtttccgaag aaaccaccac cggcgttcac cgcttgtacc
     1681 agatgatgga aaaaggcgaa cttctggtgc ctgctattaa tgtaaatgat tcggttacca
     1741 aatcaaaatt cgacaacctt tacggctgcc gcgaatcact ggccgatggc atcaaacgcg
     1801 ccaccgatgt gatgatagcc ggaaaagtgg tcgtggtgct cgggtacggc gatgtgggca
     1861 aaggatgtgc aaaatccatg cgttcctatg gcgcgcgggt tattgttacc gaaatagatc
     1921 ctatttgcgc cctgcaggcc gccatggaag gttttgaagt tacctgcacc gaagatgccc
     1981 tggccgaagg aaacatcttt gttaccacta ccggaaaccg cgatgttatt accgttgaac
     2041 acatggccaa aatgaacgat caggcgatag tgtgcaacat cggacatttt gataatgaaa
     2101 tccaggtaag taaactggaa aaattccccg gcataaagaa aattactgtt aaaccacagg
     2161 ttgataagta tatattccct gacggcaaag ctattttctt attggctgaa ggccgcctgg
     2221 taaacctggg ctgcgctacc ggacatccct cctttgtgat gagcaattcc tttaccaatc
     2281 agacacttgc acaaatggat ctgtggaaaa acaaagatgt ctataaaatt ggcgtgtacc
     2341 gcctcccaaa atacctcgac gaagaagtgg cccgtctgca cctcgaaaaa attggtgtga
     2401 aactgaccaa actttctaaa gaacaggcag attatattgg tgttcccgtg gaaggccctt
     2461 ataaagccga tcactacagg tactaatcag gcgcaacaaa attcaatatt gaatggccaa
     2521 atattgaata tcaatgtttt atcatggcga aaaagcttta tcggtttgtt gagtacctat
     2581 tctttgtggc ttttgtttcg ctggcttttt cgtgtgccaa catcgtatcg cctaccgggg
     2641 ggcctaaaga tattaccccc ccgaaagtaa aaaccaccga accgcctaat tattcggcgg
     2701 gttttaccgg aaacaaaatc aggataacat tcaatgagta tgttgtgctg gaggatcagg
     2761 caaaccaggt tgtggtttcg ccccctgtag aaaaagaagt cgaattcaat atcagtggca
     2821 aatcaataat aattgacttg cctgataata tcagggattc gattacctat accgtcaatt
     2881 tcggtacgtc catcaaagac cttaatgaag gaaatccgct gcgtaattac gaatttgttt
     2941 tctcgaaagg cagtgcggtg gattcgatga gcatacaggg acgcgtgtat gatgcactgt
     3001 tgcgcgaccc acagaacggt gtcctcgtca tgctttatga tgccggcgat gattcgttgc
     3061 ccataaaatc ccggccactg tacctggcaa agaccaatgc agaaggcaat tttgtaatcg
     3121 gtaatattaa aaaacaaaag tataagatat ttgccctcag ggatatgaat gccgatatgc
     3181 tgtttaacca gcctaccgaa tccattgctt ttgccgacag tacgcttacg cctcaataca
     3241 ttgtgaaaaa aaccgatacg gctaaaataa aggcggattc ttcttcaatc gcgcaggaca
     3301 gccttatgcc tgttgaaccg gtacgcaaca ctattctcta tctgtttaca caaaaagata
     3361 ccagtcaaaa actgttgaaa gcgcgttcgg atgaatatcg tcagtgcaaa tttgttttta
     3421 aaaacccggt taaagatttg cagattattg ttacaaacag gcaactacct gaaaactgga
     3481 gggttgatga gttttcggcc ggtaaagata cccttgtctg ctggttgaaa gatcctgata
     3541 tggatacgct gaaaatgatt tttatggata aggggattgt ttttgatact gccgaattag
     3601 ccctgaagcc caaaccgtca gaccaacaag ctgttaaa
//
LOCUS       DAOSFP010000231         3637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23580_length_3637_cov_3.249302, whole genome shotgun sequence.
ACCESSION   DAOSFP010000231 DAOSFP010000000
VERSION     DAOSFP010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23580_length_3637_cov_3.249302"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..633
                     /gene="def"
                     /locus_tag="PLI16_07310"
     CDS             64..633
                     /gene="def"
                     /locus_tag="PLI16_07310"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOH84404.1"
                     /translation="MKLPIIAYGTPLLKKKSIDIDDTFPDLKKLIADMYETMYASVGV
                     GLAAPQVGLNIRLLVVDTNPYAEDYPEGKSFKKVFINPQIIEFSGKEWSFNEGCLSVP
                     EIREDVVRHETIKIEYYDENFNFFSEEYSGIISRVIQHEYDHLEGVLFVDRLSSLKKM
                     VLKRKLTDISKGLVKTKYKMIFPLQKKHV"
     gene            646..1194
                     /locus_tag="PLI16_07315"
     CDS             646..1194
                     /locus_tag="PLI16_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84405.1"
                     /translation="MRKVIFIVSVLIGMLLIGCGQSREKMLNEITEKEKPFFSNVPVT
                     DTVKINEMVSLYTRFADKFSDDSLSPVFLYRAAGLQMNSGKNEAALASLDKIIKDHSD
                     FAKLPEAYFLKAFIYDNNIKNLKKAREAYLEFIQKFPENELADDAQISINNLGKTPEQ
                     IINEIQMKQKQHEDSLAAASKK"
     gene            1256..1726
                     /locus_tag="PLI16_07320"
     CDS             1256..1726
                     /locus_tag="PLI16_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOH84406.1"
                     /translation="MTDTKKNRRYQRLYAQLSDLAEKSAYPLSQMATINALLYHKIDY
                     FFWCGFYEVKDKHLAVVSYQGPLACQLLPQNKGVCWAAVNENKTIIVPDVEAFPGHIA
                     CSSLSKSEIVVPVRNKEGKPVAVLDVDSKEYGSFDETDALYLEKIVQLIHADTL"
     gene            complement(1732..2535)
                     /gene="cdaA"
                     /locus_tag="PLI16_07325"
     CDS             complement(1732..2535)
                     /gene="cdaA"
                     /locus_tag="PLI16_07325"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOH84407.1"
                     /translation="MICLIMLLFIADFLKFRATDFIDILLVALLLYQIYRLMKGTAAI
                     NIFLGIVAIYFFWKLVNILQMNLLSEILGQFVSVGVIALIVVFQREIRQFLLLLGSPR
                     FLNRKQKWLAFWKLNVQQSGIINLDHIARVCETLSSSKTGALIVISKKSNLEEYINNG
                     ELIDARVSEQLIENIFYKNSPLHDGAIIITGNRIVAARCVLPLTRNENFPPTLGLRHR
                     AAVGVSEITDAITIVVSEQTGNISYTKNGTLYAKINPEALKAFLELEFA"
     gene            2771..>3637
                     /locus_tag="PLI16_07330"
     CDS             2771..>3637
                     /locus_tag="PLI16_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOH84408.1"
                     /translation="MKKIIILTGLLIIVSIASGQDIHFSQYIYAPLMLNPANTGAFSG
                     DHRLTGNYRSQWKSVSRSYTTYGISYDMGILKGKLNGGILGLGIQVFNDQAGDNKLGQ
                     TMINISAAYHLPLNRNNMLTAGIQGGVAQKRINMGTMQWDSQYDPSFPDGYNASLPSN
                     ETINFNNFWYGDIGAGLLWSYTSNATTLSSNDAKKVSAGVALWHMNRPKQTFSDIATK
                     KMDMKLAVHVNSFIGFNNTNFSLLPTAAWFMQGPSNEILFGTLLRFRLNDASKYTNFV
                     TETAVAMGLFYRW"
BASE COUNT         1162 a          777 c          726 g          972 t
ORIGIN      
        1 tgataccatc agcgccacca ttattttgca atcgtacctt gaatcactaa caaaaacaag
       61 cttatgaaac ttcctataat tgcatacggc acgccccttc taaagaaaaa atcaattgat
      121 attgacgata catttcctga tttgaaaaag ctgattgccg atatgtacga aaccatgtat
      181 gcctctgttg gcgtaggact ggcagctcca caggtgggac ttaacatcag gctgctggtg
      241 gttgacacca acccctatgc tgaagattat cccgaaggaa agagttttaa aaaagttttt
      301 attaatcctc agattatcga gttcagcggc aaagaatggt cttttaatga aggatgtctc
      361 agcgtgccgg agataagaga agatgtggtc cggcatgaaa ccataaaaat tgaatactac
      421 gacgaaaatt tcaatttttt ctccgaagaa tattccggaa ttatctcacg ggtgatacaa
      481 cacgaatacg atcatctgga aggagtattg tttgttgacc ggctgagttc gttaaaaaaa
      541 atggtactca aacgcaaact taccgatatc tcaaaaggcc tggtcaaaac aaagtataaa
      601 atgatatttc ctttgcagaa aaaacacgtt taaaaattta gtattatgag aaaagtaatt
      661 tttatagtca gcgtacttat tggtatgctg cttattggat gcggacaatc aagagaaaaa
      721 atgttgaacg agataacgga aaaagaaaaa ccttttttct caaacgttcc cgtaaccgat
      781 actgttaaga tcaatgaaat ggtatctctt tataccaggt ttgccgataa attctctgat
      841 gattcgctgt cgccggtatt tctttacagg gctgccggcc tccagatgaa ctccggcaaa
      901 aacgaagcag cccttgcctc gcttgataag attattaagg accattcgga tttcgctaaa
      961 cttccggaag catattttct caaggctttt atttatgata acaacataaa aaacctaaaa
     1021 aaagcccgtg aagcatatct ggaatttatt caaaaattcc ctgaaaatga actggccgac
     1081 gatgcccaga tatccattaa taaccttggc aagaccccgg agcagatcat caatgagatt
     1141 cagatgaaac aaaaacagca tgaagactcg ctggctgccg cctcaaaaaa ataaagttca
     1201 ttatttatta taaatcgttt caatggtatt cgaaacgatt ttttttcaat ttctcatgac
     1261 tgataccaaa aaaaaccgcc gataccagcg gctgtatgca cagctgtctg atttggctga
     1321 aaaaagcgcc tatcccctct cccagatggc cacgataaac gctttgctgt atcacaaaat
     1381 agattatttt ttctggtgcg gattttacga ggtaaaagac aagcacctgg ccgttgtcag
     1441 ttaccagggg ccgctcgcct gccagttgct gccccaaaac aaaggcgtat gctgggcagc
     1501 ggtaaatgaa aacaaaacga tcattgttcc tgatgtagaa gcctttccag ggcatattgc
     1561 ctgcagctca ctttccaaat cagagattgt ggtgcccgtg cgcaacaaag aggggaaacc
     1621 ggtagccgtg cttgatgtgg acagcaaaga gtatggttcc tttgacgaaa ccgatgctct
     1681 ttatctggaa aaaattgtac agctgataca cgccgatacc ctttgaaatt atcaggcaaa
     1741 ctccagctca agaaaggctt tcagggcttc ggggttgatt ttggcatata aggtgccgtt
     1801 ttttgtatag gaaatattac ctgtttgttc tgaaacaact atggtgatag catcagtaat
     1861 ttcggacacc cccacggcgg cacggtgccg caagcctaag gtaggcggaa aattctcgtt
     1921 acgcgtcaac ggcaacacac aacgggcggc cacgatacgg ttgccggtaa ttatgattgc
     1981 gccatcatgc aaggggctgt ttttgtaaaa aatgttttca atcaattgtt ccgaaacccg
     2041 ggcatcaatt aattcgccgt tgttgatata ttcttcaaga ttgctttttt tggaaatcac
     2101 aatcaaggca ccggttttgg aagatgaaag tgtctcgcac actctggcaa tatggtcgag
     2161 atttatgatg ccgctttgtt gaacgttgag tttccaaaag gccagccatt tttgtttgcg
     2221 gttcaaaaaa cggggtgagc ccagcaaaag caaaaactgt ctgatctcgc gctgaaaaac
     2281 tactatcaaa gcaatcacac caacgctgac aaactgacct aaaatttccg aaagcaggtt
     2341 catctgaaga atatttacca gtttccagaa aaaataaatg gccacaatac ccaggaagat
     2401 attaatagcc gccgtaccct tcatcaaacg gtaaatctga taaagcagca aagcaaccag
     2461 caggatatcg ataaaatcag ttgccctgaa tttcagaaag tcagcaataa acagcaacat
     2521 aatcaagcaa atcaaacgaa taacaattca aactaatagc tattaaagat taattcatag
     2581 caaaaatata aaaaaaatga acgcaggact taaaccatta acctgaaaaa ttcatataaa
     2641 ttgttcaccc ttcgaacccg ggcttatttt tttcttcaaa aaaattttta agctaaaaaa
     2701 ccataacaat aatgaaaatt atatatcttt gattcagaaa attattaagc aggtacttac
     2761 tttttttaat atgaaaaaaa taataatact caccgggctt cttatcattg ttagcatcgc
     2821 ttcaggacag gatatacatt tttcgcagta catttatgct ccgttgatgc taaatccggc
     2881 caataccggt gcttttagcg gggatcaccg cctgacagga aactaccgca gtcaatggaa
     2941 aagtgtttcc cgttcctata ctacttacgg catctcatac gatatgggca tcctgaaagg
     3001 caaactcaac ggcggcattc tcggtcttgg cattcaggta ttcaacgacc aggcgggaga
     3061 taacaaactg ggacaaacca tgatcaatat ttcagcagcc tatcacttgc ctttaaacag
     3121 gaacaatatg ctcacggccg gcatacaggg aggcgttgcg caaaaaagaa ttaatatggg
     3181 caccatgcag tgggacagcc agtacgaccc cagtttccct gatggctaca atgcttcatt
     3241 accctcaaac gaaactatca atttcaacaa tttctggtat ggcgacatag gtgcaggcct
     3301 tttgtggtct tatacttcca acgcaaccac cctgtcatca aacgatgcca aaaaagtcag
     3361 cgcaggcgta gctttgtggc atatgaacag gccgaaacaa acgttttcgg acattgccac
     3421 caaaaaaatg gatatgaaac ttgccgtgca tgtgaattct tttattggtt ttaacaacac
     3481 caatttttca ttactgccta cggctgcctg gtttatgcag ggtccgtcaa acgaaatcct
     3541 gtttggtact ttgctgcgat tcaggctcaa cgacgcatcg aaatacacca attttgttac
     3601 agagacggct gttgccatgg gattgtttta ccgttgg
//
LOCUS       DAOSFP010000232         3632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23623_length_3632_cov_3.873637, whole genome shotgun sequence.
ACCESSION   DAOSFP010000232 DAOSFP010000000
VERSION     DAOSFP010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23623_length_3632_cov_3.873637"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..995
                     /locus_tag="PLI16_07335"
     CDS             234..995
                     /locus_tag="PLI16_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOH84409.1"
                     /translation="MATQELKKLLILFKGLVSFIIFIITSQVFTLLIPAPFTHKKKKA
                     DDFYTRLLVGYARFVIKTHFNIRVRYINKKKNTFDKPVMIVCNHQSFTDVVVTTAFDS
                     KVCVLNNNWHDNVWARFFLTKYINLFSIYKEKKLLVEELRTPVKMGCPLLIFPEGKMN
                     KDGTISRFHKGGFFVAEKLRMDIQPVVIFYSPQILKKSWFYLKHGDVLVKFLDPVTVE
                     SPGYGATYQELTDNVMRLMRTEFKQLSAQYHQGDF"
     gene            1044..2717
                     /locus_tag="PLI16_07340"
     CDS             1044..2717
                     /locus_tag="PLI16_07340"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HOH84410.1"
                     /translation="MVLNILIGLLLLTVAVILIYFLRAYKRHPAVPATDLTDYQPVQK
                     SDRMLQLGNNWCHLNSRGLYEMYIEGAPYERGIINGRLTEKLIYTQENLFIEEIKRRI
                     PNNFYLQFLKIVSLWMNRKLKGNISKEYDLEIYGVSLSASARFRMIGPAYLRILNYHA
                     AHDIAHALVNINLVGCTAFVASGKHTPDGNLLHARNFDFYAGDEFCKEKIISFYAPDR
                     GNKFAMITWGGLVGTVSGMNMQGLAITVNAAKSKMPRSISTPVTILVREMLQYAGTIE
                     EAVAIARKRRISVSESFLVSSGRDNKALIIEKTPYRTEIFEPDNGQIICTNHFQSEAL
                     KKDPMNIRQINDTASMYRYQRTRELFERSGQITVEKAVGILRNRLGLEDKPIGNGNEY
                     TLNQLLAHHAIIFKPGELKMWVSSGPFNLGEFLCYDLNTVFGKPSSIKDTCNIITENL
                     VIPGDPMLTNGEFEKFERFKQIRKELLATHENQGIVNALIEEMIGLNPEYFLSYSTAG
                     DYLRRRKDYTHATSYYRIALQKEIPTKKEKEEIRKHLDLCEKKTCATDG"
     gene            2710..>3632
                     /locus_tag="PLI16_07345"
     CDS             2710..>3632
                     /locus_tag="PLI16_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH84411.1"
                     /translation="MDDLADIKAVFDELHCCVIMPTYNNAGTIGKVLSEVLEYTGNII
                     VVNDGSTDKTGKILESFSGIHILDNKKNKGKGYALRKAIRYAHSMGFRYGISIDADGQ
                     HFASDLPAFLKKIMEVPGAIIIGSRNLQTENMPAKNSFGNKFSNFWFYIETGRKLPDT
                     QSGFRAYPLYLLHKKRFFGRKYEFEVEVLVRSAWKGIKTIPVPINVYYPPKTERISHF
                     RPFVDFFRISVLNTILVLAAFLVVKPFSFLRALNKKNIHDFFQKEFVQSPDKDYKIIA
                     SVMVGVFFSVTPFWGWQVWIALLVALVFRLN"
BASE COUNT         1124 a          763 c          780 g          965 t
ORIGIN      
        1 gccagagtat atattggata accttggatg atgaaaatgg tcagaaagtt actcaaaaat
       61 tcatcaaaat gtaaggcttg atttttcata aaatgttcag gtagattttt cagggaatga
      121 ttttgtttac ctttgccggc acaacggcga cattgttttg tgcacaacag gaaaatatcc
      181 gttgaaaaga aatcggttca tttacacaaa aagattgact ctccagattt taaatggcaa
      241 cacaagaact aaaaaaacta ttgatactct tcaaaggact ggtttcgttt ataattttca
      301 tcatcacttc acaggtattt accttattga ttcctgcgcc ttttacacac aaaaaaaaga
      361 aagctgatga tttttacacc cggctgttag tagggtatgc acgctttgtg attaaaacgc
      421 attttaatat ccgggtacga tatatcaata aaaagaaaaa tacctttgat aagccggtaa
      481 tgatagtatg caaccatcag tcgtttaccg atgtagtggt tactacggct ttcgattcaa
      541 aagtttgtgt actcaacaac aactggcatg ataatgtctg ggccaggttt ttccttacaa
      601 aatatataaa cctgttttct atttacaaag aaaaaaaact gctggttgaa gagctcagga
      661 cacctgtaaa aatgggatgt ccgttactga tatttcccga aggtaaaatg aataaagacg
      721 gaacgatatc gcgttttcac aaaggaggat tttttgttgc agaaaaactc aggatggaca
      781 tacagcccgt agtgatattt tattcgccgc aaatactgaa aaaaagctgg ttttacctca
      841 agcacggtga tgtcttagta aaatttcttg atccggtaac cgtggaaagt cccggatatg
      901 gcgccactta ccaggaactc actgataatg tcatgcggct aatgcgtact gaattcaaac
      961 agctcagcgc acagtaccat caaggtgatt tttgaacagc ggcaatccaa aaaaagataa
     1021 ttttgtaagc taaagataat ggaatggtat tgaatatact tattggcctg ttactgctga
     1081 ctgttgctgt gattctaatt tattttttac gtgcttacaa aaggcatccg gcggttcctg
     1141 ccacagacct gaccgattat cagcccgtgc aaaaatccga ccgtatgctt caattgggca
     1201 ataattggtg ccacctcaat tcgagagggc tttatgaaat gtacattgaa ggagcgcctt
     1261 atgagcgcgg catcattaat ggccggctga ccgaaaagct gatctatact caggaaaacc
     1321 tttttattga agagataaaa agaaggatac ccaacaactt ttaccttcag tttcttaaaa
     1381 ttgtttcttt atggatgaac cgcaagttaa agggaaatat cagcaaggaa tacgatcttg
     1441 aaatttacgg ggtttctctt tcggcatcag ccaggttccg gatgataggc cctgcttatt
     1501 tgcgcatact caattaccat gcggctcacg atatagccca cgccctggtg aatatcaacc
     1561 tggtcgggtg cacagcattt gttgcttcag gaaaacacac gcccgacggc aacctgttac
     1621 atgcacggaa ttttgatttt tacgccggtg atgagttttg caaagagaaa attatttctt
     1681 tttacgcgcc cgaccggggc aacaaattcg ccatgatcac ctggggcggc cttgtaggca
     1741 cagtttcggg catgaatatg caaggtctgg ccattacggt aaatgcagcc aaatcaaaaa
     1801 tgccccgcag tatctccaca ccggttacca tactggtcag ggaaatgctg caatatgccg
     1861 gcactatcga agaggccgtg gcaattgccc gcaagcgcag aatttcagta tccgaatctt
     1921 ttctggtaag ctccggcagg gataacaaag cactgattat cgaaaaaact ccttaccgga
     1981 ccgaaatttt tgaaccggac aacggccaga ttatctgcac caaccacttt cagtcggagg
     2041 cccttaaaaa agacccgatg aacatacggc agatcaacga tacggcctca atgtaccggt
     2101 accaaagaac ccgtgagttg tttgaacgaa gcgggcaaat tacagttgaa aaagccgtag
     2161 ggatactaag aaatcgccta gggttggagg ataagccgat aggtaacggc aacgaataca
     2221 cccttaacca gctgctggcc catcacgcca tcattttcaa gcccggcgaa ttaaaaatgt
     2281 gggtttcatc agggccgttc aaccttggag aattcttatg ttatgacctg aatacggtat
     2341 tcgggaagcc ctcatccatc aaggatacct gtaatataat cacagaaaac cttgtaatac
     2401 cgggcgatcc catgctgacc aacggagaat ttgagaaatt tgaaagattc aagcaaatta
     2461 ggaaagagtt gctcgcaact catgaaaatc aaggcatagt gaatgctttg attgaagaaa
     2521 tgattggcct taaccctgaa tactttttat cctacagcac cgccggcgat tacctccgta
     2581 ggagaaaaga ctatacacat gcaacttctt attatcgtat agccctccaa aaagaaatcc
     2641 ctaccaaaaa ggagaaagag gaaatccgca aacaccttga tctttgtgaa aaaaaaacct
     2701 gcgcaaccga tggatgacct ggctgacata aaagctgttt tcgatgaact gcactgttgt
     2761 gtgattatgc cgacgtataa caacgccggc accatcggca aggtattgtc ggaggtgctt
     2821 gaatacacgg gcaacatcat cgttgttaac gacggatcga ccgataaaac agggaagatt
     2881 ctggaaagct tttccggcat tcatattttg gataataaaa aaaacaaagg caagggctat
     2941 gcgctgcgaa aagccatcag gtatgcacat agcatgggtt ttcggtatgg gataagcata
     3001 gatgccgacg gacagcattt cgcatccgac ctgcctgctt ttctgaagaa aataatggaa
     3061 gtgcctggag caatcatcat cgggtcaagg aacctgcaaa cggaaaacat gccggcaaaa
     3121 aacagttttg gcaataagtt ttcgaacttc tggttttata ttgaaaccgg ccgtaaactg
     3181 cccgacacgc aatcaggctt cagggcctat cccttgtacc tgttacacaa aaaaagattt
     3241 tttggccgga aatatgaatt tgaagtggaa gtgctggtac gttcggcctg gaaagggatc
     3301 aaaacaatcc ccgtacccat caacgtttat tatccgccaa aaacagaacg catctctcat
     3361 ttcaggccat ttgtggattt tttcaggatc agtgttttaa atacgatact ggtccttgcg
     3421 gcattcctgg ttgtgaaacc tttctcattt ctcagggcat taaataaaaa aaatatccat
     3481 gatttttttc aaaaagaatt tgtccagagc cctgataaag actacaagat tattgcttct
     3541 gtcatggtgg gcgttttttt cagcgttaca cctttctggg gatggcaggt atggatcgcc
     3601 ctgctggtag cattggtatt cagactcaac aa
//
LOCUS       DAOSFP010000233         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23777_length_3616_cov_4.533277, whole genome shotgun sequence.
ACCESSION   DAOSFP010000233 DAOSFP010000000
VERSION     DAOSFP010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23777_length_3616_cov_4.533277"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PLI16_07350"
     CDS             complement(<1..258)
                     /locus_tag="PLI16_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH84412.1"
                     /translation="MKNDTITYHKEAIRKMMDYINENLFNRTKLSIKELGEVALVSPR
                     HAARIFKADTGETPGQYILRLRLEAARQMLISGMSIWETSEK"
     gene            476..1570
                     /locus_tag="PLI16_07355"
     CDS             476..1570
                     /locus_tag="PLI16_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOH84413.1"
                     /translation="MAKYIYQYPDWPDFYWDEKEIQVILGTVRHLQGRLWGQINALGF
                     SIKEENTLTTLTLDVLKSSEIEGEILNYDQVRSSIARKLGLKCAGMVNTSRYVDGVVE
                     MMLDATQHYRKPLDEERLFGWHAALFPTGRSGMYKIDTACYRKGEMQVVSGAMGKEKI
                     HFQAPPPARVPGEMKAFLKWLNKDTKLDMVLKSAIAHFWFIIIHPFDDGNGRIARAIS
                     DLLLTRSDESPQRFYSLSNQILKERKLYYKVLQKVQFSDGDITEWLVWFLHCLYRALQ
                     STEESIQGVIRKAEFWDKHKETLLNSRQRLMLNKLFDGFDGKLKTSKWAKMAKCSPDT
                     ALRDIKDLMDKGILKQEKSGGRSTNYILNL"
     gene            1674..3359
                     /locus_tag="PLI16_07360"
     CDS             1674..3359
                     /locus_tag="PLI16_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84414.1"
                     /translation="METKSENKVCFNVLQAISRVFKASKDSKLKNELFKKINPKLELL
                     SGYFKVTKKQAFLLANVYALLCNGRTFELPDLSHHLGVEPVDLLGLQEDLDELNSKGI
                     LLKKRNRNGFYGTTNFVYFMEDTISKAILKNSPIQHIKPLTCKDIFEVLNKIYLFSEE
                     RNEDKMTTEELFSQMEQILDANSHLGLIQKVKNLELCNDDTYLFYYLCWKTITGNESI
                     DFNNVVDVIFDDGYQKARYSQEFYSGKNILMSQKFVTLIEGTFFNDSDIRLSPKSIEL
                     LKSENIALFSRKISRDNIISVADIGAKDLFFGSSENRQLEMIENTLKSDNFDQLQQRM
                     KQKHMPAGVALLLYGAPGTGKTESVYQIARKTGREIMHVDISQAKSMWFGGSEKRIKQ
                     IFTDYREFVEQSKLCPILLFNEADAILSRRKDSSYSIVSQTENAMQNIILEEMEKLNG
                     IMFATTNLTANLDSAFERRFLYKVEFFPPDFNARKLIWKSKLIGLSEKDGALLADKFH
                     FSGGQIENIARKCEMFEILNGRIPDINKIVEFCEEELIDKKQHITVGYKINHP"
BASE COUNT         1136 a          683 c          782 g         1015 t
ORIGIN      
        1 cttttccgaa gtttcccaaa tgctcattcc tgaaatcagc atctgtcttg ccgcttccag
       61 acgtaaccta agaatatact gtccgggagt ttcaccggtg tccgccttaa agatccttgc
      121 agcatgtctg ggagaaacca gggctacttc gccaagttct tttatactta gttttgttct
      181 gttgaacaaa ttttcattga tgtaatccat catttttctg atggcctctt tgtggtatgt
      241 aatggtatcg tttttcatct gattttattt ttcagcaaag atagggtgat cttctgtttg
      301 cattattact gtaaaggaac aaaatgtctg ccccggtaga ataatctgat ttttcgttat
      361 tggtgcgggg ttttctgttt ataaaaaaaa tagtataatg caatgattaa tttcaatatt
      421 ctcattaaaa atgcggagaa tattttgtat atttgccgca taaagaggtt atcagatggc
      481 aaaatatatc tatcaatacc ccgactggcc tgatttttac tgggacgaga aagaaatcca
      541 ggtaatactg ggcaccgtac ggcacttgca gggaagactt tgggggcaga ttaacgcatt
      601 ggggttttca ataaaggaag aaaacacgct caccacgctg acactcgatg tgttgaaatc
      661 gtcagaaata gaaggcgaaa tactcaatta tgaccaggtg cgttcatcca ttgccagaaa
      721 acttgggctg aaatgcgcgg gaatggtaaa taccagcaga tatgtagatg gtgtggtgga
      781 aatgatgctt gatgcgacac agcattaccg gaaaccgctc gatgaagaac gtttatttgg
      841 ttggcatgcg gctcttttcc ccaccggaag aagtggtatg tataaaattg ataccgcttg
      901 ctaccgtaag ggcgaaatgc aggttgtatc gggcgctatg ggcaaagaaa aaattcattt
      961 tcaagcgcct cctcctgccc gggtgcccgg cgaaatgaag gcgtttttga aatggcttaa
     1021 caaggacacc aagctggata tggtgttgaa atctgccatt gcgcattttt ggttcatcat
     1081 tattcatcct tttgatgatg gtaacgggcg tatagcaagg gcaatttccg atttattgct
     1141 gacccgttct gatgaaagcc cgcaacggtt ttacagcctt tcaaaccaga ttttaaagga
     1201 aagaaaactt tattataagg tcttgcaaaa ggttcagttt tctgatggcg atataaccga
     1261 atggctcgta tggtttctgc actgtttata cagggcactg caaagtaccg aagagtctat
     1321 acaaggcgtt atccgcaagg ctgagttttg ggataagcac aaggaaaccc tgttgaacag
     1381 ccgtcagcgg ctgatgctca acaagctttt tgatggtttt gacgggaagt taaaaacctc
     1441 gaaatgggca aaaatggcta aatgctctcc cgatacggct ttgagagata tcaaagacct
     1501 gatggacaaa ggtattttaa aacaggaaaa atcaggcggg aggagtacta attatattct
     1561 taatttgtaa agcggggact gctcacttcc ttaaaaaatt tttctgattt ttttgaccac
     1621 gacacatttt ggcgtagtct cattgttatt ttgttttcat aaaagaaata aaaatggaaa
     1681 caaaatcaga aaacaaagtg tgttttaatg tgctgcaagc catcagcagg gtgttcaaag
     1741 catcaaaaga cagcaaactc aagaatgagc tttttaaaaa aatcaacccc aaactcgaac
     1801 tgctgtccgg atattttaaa gtaaccaaaa aacaggcttt cctgcttgca aatgtctatg
     1861 cccttctatg caatggcagg acttttgaat tgcccgatct gagtcaccac ctcggagtgg
     1921 aacctgttga tttgcttggc ctgcaggaag atttagacga gctgaacagc aaaggtattt
     1981 tattaaaaaa aagaaacaga aacggattct atggcacaac aaattttgtt tattttatgg
     2041 aagataccat tagcaaagcc atcttaaaaa attcaccgat tcagcatatc aaaccgctga
     2101 catgtaagga tattttcgaa gtgttaaaca aaatatacct tttcagcgaa gaaagaaatg
     2161 aagataaaat gacaacagag gagttgtttt ctcagatgga gcagatattg gatgccaatt
     2221 cccacctggg tttgattcaa aaagtgaaaa acctggaatt gtgtaatgac gacacctatc
     2281 tgttttatta cctctgctgg aaaaccataa caggaaatga atccatagac tttaacaatg
     2341 tggttgatgt aatttttgat gacggatacc agaaggcgcg atactcgcag gagttttatt
     2401 ccggcaaaaa cattctgatg agtcaaaagt ttgtaacgct tatagaagga acttttttca
     2461 atgattccga catcagatta agtcccaaaa gtattgaatt gcttaaatca gaaaacattg
     2521 cccttttttc aaggaaaata agcagggaca atattatttc ggttgccgat attggggcaa
     2581 aagacctctt tttcggcagt tcagaaaacc gtcagctcga aatgatagaa aacacgctga
     2641 aaagcgataa cttcgaccag ttgcagcagc gcatgaaaca gaaacacatg cccgcaggag
     2701 tcgctttgct actatatggt gcaccgggaa caggcaaaac cgagtctgtg tatcagattg
     2761 cccgaaaaac aggccgtgaa attatgcatg tggacatcag ccaggctaaa tccatgtggt
     2821 ttggcggaag cgaaaaaaga ataaaacaga tttttaccga ctatagagag tttgttgaac
     2881 agagcaaact ctgccctatt ctgctgttca acgaagccga tgccatactg tcaagacgca
     2941 aagacagcag ctattccatc gtatcacaga ccgaaaatgc catgcagaac atcattctgg
     3001 aagaaatgga aaagcttaat ggaattatgt ttgccacaac caacctgact gcaaacctgg
     3061 acagcgcttt tgaaagaagg tttttgtata aagtagagtt ctttccaccg gatttcaatg
     3121 cccggaagtt aatatggaaa tctaagttaa tagggctttc cgaaaaggat ggtgcattgc
     3181 tggccgataa attccatttc tcaggagggc agattgaaaa catcgcacgg aaatgcgaga
     3241 tgtttgaaat cctgaatggg cgtatcccag atatcaataa aatcgtggag ttttgcgaag
     3301 aagaactgat agataaaaag cagcacataa cggtaggtta taaaattaat cacccataat
     3361 aaatcgtttc cgtttttaat gataacatgc agaagcatcg ttatgaccgt ccctttcata
     3421 tacttttttg cctgtccaaa aagtacaagt aataactctt taaaaagata cataatttga
     3481 gttttgcatg ttttttttaa gtcacactga gccggacata gcgcagcaaa gagggttgag
     3541 tccgtgtcga agtgtgacat gcaacggtcg tcttcgacaa tcttctcctt ccactttcgt
     3601 ttcaggagaa tctgct
//
LOCUS       DAOSFP010000234         3615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23784_length_3615_cov_3.957303, whole genome shotgun sequence.
ACCESSION   DAOSFP010000234 DAOSFP010000000
VERSION     DAOSFP010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23784_length_3615_cov_3.957303"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..756)
                     /locus_tag="PLI16_07365"
     CDS             complement(91..756)
                     /locus_tag="PLI16_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOH84415.1"
                     /translation="MDSKLLANIVLLGIFLYAATGNLLAWWLSKLKWLPLTKPLLMPV
                     LAIYYVMNAPQIHWILVIALFFAFLGDVFLIWPEKRWLFTGGLLSFAIMQILYIVFIT
                     VYQITIVNWSMVVSLAALGFMAIGGMIFFMLYKYLGSMKIPVVVYILLVVSVGFLCFI
                     NMTGNFNKYYLFQFIGAMFFMVSDTILAFISFRKPFPYANLLLMAMYINAQLFLFAGF
                     INV"
     gene            883..3087
                     /locus_tag="PLI16_07370"
     CDS             883..3087
                     /locus_tag="PLI16_07370"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH84416.1"
                     /translation="MIIFNSFQLTGFSQVVVINEVCTSPHQGPTGSSVNANSLYNTSS
                     LEQPPENREWIELYNPHPCDTVDISCYTLASNMYQPDYTGSGTNNWGAFTFPTGTKIP
                     PLKFLVVGGNNSQVPVLDFDLNYYRHNTFGVIYLDGDETRWFLRDEYGWIALYNPAGT
                     PVDAVYWDAYGDPANLYSQEEYSHSIVTATTCSGTQTLAPARNIAGIEYVGMCQPGTD
                     VSFQRVLDGGPVWHNGPVTTTPHYCNGPCTAPPVVSATVENESCAGNDGSIALSIQDG
                     HTGPYTINWLNPAGQHSNDIGNLQAGTYIVQVADAYNCFIVYDTITLSSDPDPTVSFT
                     SLINETCDKNNGSLQAAVTDGNLPVSYQWNITGTGNTPAIGNLSAGTYSVTITDNLGC
                     TASNSAILENFPGPELNIDSVRNEMCSTSDGAIFLTVNGGTTPFSYTWNTAASQNAPQ
                     LTGIPAGNYSVTITDANSCSASASTVITNTPPPAATITDIQADTCRKKTGAATIIASG
                     GNPPYSFFWSFDSTLYTNAVTNLSEGPYTVSVSDNFCTTVVSFTIPLIPGPTADFSIY
                     PAVATIDDPPFRFVNLSSGIIDQYIWDFGDKRYSYEINPYYKYSDTGHYHVKLTVKSY
                     AGCIDSIIKQAVVMDRITLFVPNCFSPNNDGVNDYFIIGGQNITEYELLIYNRWGELV
                     YKTNDLSQSWDGRYQGHVVPEGIYGWTINYSEDWSGIYTTPQCRRGMVNVIR"
     gene            3334..>3615
                     /locus_tag="PLI16_07375"
     CDS             3334..>3615
                     /locus_tag="PLI16_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOH84417.1"
                     /translation="MITLGLHDLTLEEIYQILFHKAEIEIHPEALQRVNNCHSFLAEF
                     SKDKVIYGINTGLGPMAPYKIEKENQTQLQYNAIRGHSSGCGDPLPDKFA"
BASE COUNT         1095 a          878 c          713 g          929 t
ORIGIN      
        1 attttggtgt tatcatctgg ggtccagcta taaacaaaca cctctacatg atcctccgcc
       61 gtaatggtaa aagttgcttc tttcataatt ttaaacattt ataaatccag caaataagaa
      121 caattgtgcg ttgatgtaca tagccataag caataaattg gcatacggaa aaggcttcct
      181 gaaacttata aaagccagta tagtgtcgga aaccataaaa aacatagcgc caataaactg
      241 aaacaggtaa tacttattaa agtttccggt catatttata aaacataaaa atcccaccga
      301 tactaccagt aaaatataca ctacgacagg tattttcatg ctaccgagat acttatacag
      361 cataaaaaat atcatgcctc ctatggccat aaaacccaac gctgccagac taactaccat
      421 cgaccagttt acgatggtta tctgataaac cgtaataaaa acaatataca aaatctgcat
      481 aattgcaaaa gacaacaacc cgcccgtaaa caaccatctt ttttcaggcc agataaggaa
      541 aacatccccc agaaaagcaa aaaacaatgc tattaccaat atccagtgaa tttgaggggc
      601 gttcattacg taataaattg ctaaaactgg catcagcaag ggtttggtta agggcagcca
      661 ttttaatttg ctgagccacc aggccaatag gttgcctgtt gcagcataca gaaaaatacc
      721 tagcagaaca atgttggcca gaagtttgct atccattaat ttggtttttg acaaacaaat
      781 atatcaatat taagttaaaa tcaaataatt tgtgctcttt tatttaaatt tgtaacaaat
      841 tttccagtaa aaatgttcag aaactgtcat atattatttt ttttgataat ctttaacagt
      901 tttcagttga ccggtttttc gcaggtggtg gtcatcaacg aagtgtgtac cagtccgcat
      961 caggggccta caggatcttc cgtcaatgct aattctctgt ataatacctc ttcgctggaa
     1021 cagccccccg aaaaccgcga atggatagaa ctttataatc cccacccttg tgataccgtt
     1081 gatatcagtt gttacaccct tgcttctaat atgtatcagc ccgactacac cggaagcggc
     1141 acgaacaact ggggagcctt tacttttcca accgggacta aaatccctcc cttaaaattt
     1201 cttgttgttg gaggcaacaa ctcacaggtt cctgtcctgg atttcgactt aaattattac
     1261 cggcataaca ctttcggggt aatttacctt gacggtgatg aaacccgctg gtttttacgc
     1321 gatgaatacg gctggatagc cttatacaat cctgcaggca ccccggttga cgcggtgtac
     1381 tgggatgctt acggtgatcc ggccaatctt tacagccagg aagaatattc gcacagcatt
     1441 gttaccgcca caacctgcag cggcacgcaa acactggcgc cagcgcgcaa cattgctgga
     1501 attgaatatg tgggaatgtg ccagccaggc accgacgtat cttttcaacg ggtgctcgac
     1561 ggcggcccgg tatggcataa cggccctgtg actaccactc ctcattattg caacggtccg
     1621 tgcacagctc cccctgttgt gtctgccact gtcgaaaatg aaagctgtgc aggaaacgac
     1681 ggcagcatag cactaagcat acaggacgga catacaggcc cttacactat caactggtta
     1741 aatcccgcag gacagcacag caatgacatc ggtaatcttc aggcagggac ttatatagtg
     1801 caggtagccg atgcatacaa ttgcttcatc gtttacgata cgattaccct gagctccgac
     1861 cctgatccaa cagtaagttt cacatcattg attaatgaaa catgcgacaa aaataacggc
     1921 tctctgcaag ccgccgtcac cgatggaaat ctacctgtct cttaccaatg gaatataacg
     1981 gggaccggaa acacacctgc catcggcaac ctgagcgccg ggacttattc cgtaacgata
     2041 accgacaacc tgggatgtac ggccagcaac agcgctattc tggaaaattt tcccggccct
     2101 gaacttaaca ttgacagtgt ccgcaacgaa atgtgcagca cctccgacgg tgcaatattt
     2161 ttgaccgtta acggaggaac caccccattt tcttacacat ggaatacggc ggcaagccaa
     2221 aacgcaccgc aactcacggg aataccggca ggaaattatt ccgtcaccat taccgatgca
     2281 aacagctgtt cggccagcgc cagcactgtt attaccaaca caccgccgcc tgccgcaaca
     2341 ataacagata ttcaggccga cacatgccgg aaaaaaaccg gagccgccac tattattgcc
     2401 agtggtggaa acccgcccta tagttttttc tggagtttcg actccaccct ctataccaac
     2461 gcggttacca atctttctga aggaccatat accgtttcgg taagcgacaa tttttgcacc
     2521 accgtagttt cttttactat cccgcttatt cccgggccaa cagcagattt cagcatttat
     2581 ccggcagtgg ccaccatcga cgatccaccc ttccggtttg taaacctttc ctccggaatt
     2641 atcgatcagt atatatggga tttcggagac aaaaggtact cttatgaaat aaacccctat
     2701 tacaagtaca gcgatacagg ccattaccat gtgaaactaa ccgtaaaaag ctatgccgga
     2761 tgcatcgact ctatcattaa gcaagccgtg gtaatggaca gaattaccct gtttgttccc
     2821 aactgttttt cgccgaataa cgatggtgta aatgattatt ttatcattgg aggacaaaat
     2881 attaccgaat acgaattgct tatttacaac cggtggggcg agctggtata caaaaccaat
     2941 gatctcagcc aatcatggga tggccggtac cagggccatg tcgttcccga aggtatttat
     3001 ggctggacta ttaactattc ggaggattgg tccggcatat ataccactcc gcagtgccgg
     3061 cgaggaatgg tgaacgtcat ccggtagcgg cgtcaaaccc gtgctgagtg tcccggcaaa
     3121 gtcacgcagc agaaacaaaa aacttacacc ctgaaaagcc ggctttcgat aggttttagc
     3181 cttttaatcc tctctgaaac agcgaaatat cggaagtggc aagtactaat gcttttctta
     3241 ttccacaata atttcggttt gtaaatttat ttttgcgaaa tttgcacccg caaaataaat
     3301 tattatctat cggtaattaa aaacaaaaac tacatgatta cacttggttt acatgatctc
     3361 acgctggaag aaatttacca gatacttttt cacaaggccg aaatagaaat ccatcccgaa
     3421 gccctgcaaa gagttaacaa ttgtcatagt tttcttgcag aattcagcaa agacaaagtt
     3481 atttatggca ttaatacagg tctggggcct atggcacctt ataaaattga aaaagagaat
     3541 cagacacaac tgcaatataa tgctattcgc ggccactctt caggttgcgg cgaccccctg
     3601 cccgacaagt ttgcc
//
LOCUS       DAOSFP010000235         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23795_length_3614_cov_3.034279, whole genome shotgun sequence.
ACCESSION   DAOSFP010000235 DAOSFP010000000
VERSION     DAOSFP010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23795_length_3614_cov_3.034279"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="PLI16_07380"
     CDS             complement(<1..717)
                     /locus_tag="PLI16_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84418.1"
                     /translation="MRKHLAIHYNPSQLISGAGANSRNTFDTCSTAIYSYKTLTKVNN
                     NKVKNTHIGVYCREATTSSYVINSTFKNNISIGVEFINILPLLKKITIKNDSILDPTM
                     GIYLSNITSQPTNPALKTTVSNNYITNVKNSSVYGIRLEKCDYVEAYCNKITRAAIPA
                     TAYRLLIRGIQIEQCANALIHDNTSERLGLSVKGAGSLLGTQFKCNTSDGSYIGFYFD
                     AAYGTVQTALSSQGIATLPND"
     gene            1652..3103
                     /locus_tag="PLI16_07385"
     CDS             1652..3103
                     /locus_tag="PLI16_07385"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOH84419.1"
                     /translation="MKKTVLTIAIAVVSSLATIFAFRFADKKNHEIQTYYQNQTPAQT
                     VSMSGRMPTTGPDFVVAAEKTVHAVVHIQTQYYYKNSYYDQFFGYDPFFNFFNKRNYS
                     NQPIVTTGSGVLISENGYIVTNNHVVSDASKIEVTLNDKRTYTATVVGSDAGTDLALL
                     KIEDTNLPFVTYGNSDEVKIGEWVLAVGNPFNLTSTVTAGIVSAKARDINIWGNNSTV
                     ESFIQTDAAINPGNSGGALVNTNGELIGINAAIASNTGSYTGYSFAIPSNIVRKIISD
                     ILDFGDVQRAFLGVNFTDITSKLAAAHDIKDMKGVFVESVYEGGAADKAGIQKGDIIV
                     KIDNSPITGSSELKEIIVRHRPGEKINVSVIRDNEIKQMSLTLENSEGGTALLKSGEA
                     GSGTVLGATLKEITDKEKIGLGLRSGVKITKLEKGKLSGAGIREGLIITRIDNQQVKT
                     TDDVVRLLGNKKGTVLIEGIYTNGMQAYFSFGL"
     gene            3253..>3614
                     /locus_tag="PLI16_07390"
     CDS             3253..>3614
                     /locus_tag="PLI16_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOH84420.1"
                     /translation="MRQLKITKSITNRETASLDKYLQEIGREELITADEEVKLAQQIK
                     AGNQEALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAKRFDETRGFKF
                     ISYAVWWIRQSILQALAE"
BASE COUNT         1135 a          760 c          704 g         1015 t
ORIGIN      
        1 atcatttggc aacgtggcaa ttccctggct tgacaatgct gtctgtactg taccgtaagc
       61 ggcatcgaaa taaaacccga tataactgcc gtccgaagta ttgcatttaa actgcgtgcc
      121 aagcaacgaa ccggcccctt taacactaag cccgagccgc tcgcttgtat tatcatgtat
      181 cagcgcattg gcgcattgtt ctatctgaat tcctctgatc aataaacggt acgcggttgc
      241 agggatagcc gctcgggtaa tcttattaca atatgcttcc acataatcgc atttttctaa
      301 acgtatccca taaaccgagc tgtttttaac attggtaata taattattac ttacagttgt
      361 tttcaaagca ggattggtag gctgtgaagt tatattcgac aaataaatac ccatggttgg
      421 atccagaata ctgtcatttt taatagttat tttttttaat aaaggtaaaa tattaataaa
      481 ctcaacccct attgaaatat tgtttttaaa agtactattt attacataac ttgaggttgt
      541 tgcttcgcgg caatacactc cgatatgggt gttttttact ttattattat taaccttagt
      601 caacgtttta taagaataaa ttgcagtact gcatgtatca aatgtattgc ggctgttagc
      661 tcctgctccg cttattagtt gcgacgggtt gtaatgtatt gccaagtgct tcctcatgtt
      721 tggttaaaat ttgtttttca ttaccgtgat aatatttttt gcctgcatgg gaggcatttt
      781 atttaaacaa ccatcaactt gcaagttgaa ataaatatta tatcttaaat ttccggctaa
      841 tgtataacaa atctttatta aaaacaagaa tccttatata attatttttc attattgcat
      901 acagccctat ttatagtcat atagcggttt tattatccga aaacaattaa ggcaaagctt
      961 atagtggcga aaggatatat atacaaataa tgataatttc tttttttgag gtgttggtag
     1021 aaaaaaatac gactcaaagt tatcggaaaa taacatgttt tcttttaaac acaattttga
     1081 aagaagactt tagcagggcg gcgccccgaa atatttgtaa gaagcggtac ccctcagaaa
     1141 agtaaggtac agcccgcttg gccggcagac aggagtatcg taatataata tccatcactc
     1201 aaataataat taaaaatcat atgtttttca atgtcttttt gtcaaattcc ctgcatcaaa
     1261 acaatttctt attacggtgt aaaaacaaga tgaatttatc attacctaaa gaatttggac
     1321 ataactagga gtttaatatt ccggtacgct ataccttgat tttcgcactt attgttattt
     1381 tacaaatatt acggtgctct gcacctaaat aacaatatta gaaaaaaatg atagggttgt
     1441 gtattagtag ctaatgcatg cgtgatctgg ggttttattt tcattattaa atattattag
     1501 cacctcaaaa aaatggttta gcccaaataa tccaccccgc attttttata gaaaaattat
     1561 actatcatta agtttcagac gttttttatt caaccctgtc aaaatttttt aggtataaaa
     1621 tttgataaga aacagccaaa aattcagcct tatgaaaaaa accgttttaa caatcgctat
     1681 cgcagtagta tcaagccttg caaccatctt tgcttttcgg tttgccgaca aaaaaaacca
     1741 tgaaattcag acatattacc agaatcaaac acctgcccaa actgtcagca tgtcaggcag
     1801 gatgccaacc acaggcccag attttgtggt tgcggccgaa aaaaccgtgc atgccgtagt
     1861 gcatatacaa acgcagtatt actataaaaa ctcgtattac gaccagtttt ttggctacga
     1921 cccgtttttc aacttcttca acaagcgcaa ctacagcaac cagccaatag ttaccacggg
     1981 ctccggcgta ctgatttccg aaaatggata tatagttacc aacaaccacg tggtgagcga
     2041 tgccagcaaa attgaagtta ccctgaacga caaaagaaca tacactgcca ccgtggttgg
     2101 cagcgatgcg ggcaccgacc tggccctgct gaaaattgaa gacaccaacc tgccctttgt
     2161 tacctacgga aattccgacg aggtaaaaat cggcgaatgg gtgctggccg tgggcaaccc
     2221 gttcaacctg acttcaacgg ttacggccgg tattgtcagc gccaaagccc gcgatatcaa
     2281 catctggggc aataactcca ccgtggaatc ttttattcag accgatgctg ccatcaaccc
     2341 gggaaacagc ggcggggcgc tggtgaatac caatggcgaa cttatcggca tcaatgcagc
     2401 catcgccagc aataccggct catacaccgg atattccttt gccatacctt caaatatcgt
     2461 ccgaaaaatt atctccgaca ttcttgattt cggcgatgta caaagagctt tcctgggtgt
     2521 caattttacc gatattacca gcaaacttgc tgccgcacac gacattaagg atatgaaagg
     2581 cgtttttgtc gagtcggttt atgagggagg cgctgccgac aaggcgggta tccaaaaagg
     2641 cgacatcatc gtgaaaatag ataattcacc catcaccggg tcttccgaac tcaaggaaat
     2701 cattgtgcgg caccgccccg gcgaaaaaat caatgtgagt gtgatccgcg ataatgaaat
     2761 aaaacaaatg agcctcaccc tcgaaaacag cgaaggcggc actgccttgc tgaaatcagg
     2821 cgaagctggc agcggaacag tgctcggcgc caccttaaaa gaaattaccg acaaagaaaa
     2881 aatcggcctc ggactgagaa gcggcgtaaa aatcaccaag ttggaaaaag gcaaactctc
     2941 tggcgccggt atcagagaag gcctgataat cacacgaatt gacaatcaac aggttaagac
     3001 taccgatgat gtggtcaggc tgttaggaaa caaaaaaggc accgtgctga ttgaaggcat
     3061 ttataccaac ggcatgcagg catatttttc gtttggattg taaccaatca cggctgattt
     3121 tcgtttaact tcccgtgcag catcaaagaa aaatattttg ctgttaccga aaatttttgt
     3181 aactttgtac ccgtttccca tcaacattag taaattattg actatcaaat gattataaac
     3241 taatcctgta taatgagaca acttaagatt accaaatcga tcaccaacag ggaaacagcc
     3301 tcccttgaca agtatttaca ggaaattgga cgtgaagagt tgatcactgc tgacgaagaa
     3361 gtaaaacttg cccaacagat taaagccggc aaccaggaag ctctggaaaa gttgaccaaa
     3421 gccaacctgc gttttgtggt atctgtggcc aaacagtatc agaatcaggg attaagcctt
     3481 cccgacctga ttaatgaggg caacctgggc cttatcaaag cggccaagcg ttttgacgaa
     3541 accaggggtt tcaaatttat ttcttatgca gtatggtgga tacgccagtc gatattgcag
     3601 gcgctagccg aaca
//
LOCUS       DAOSFP010000236         3607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23863_length_3607_cov_3.534347, whole genome shotgun sequence.
ACCESSION   DAOSFP010000236 DAOSFP010000000
VERSION     DAOSFP010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23863_length_3607_cov_3.534347"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /gene="gldE"
                     /locus_tag="PLI16_07395"
     CDS             complement(<1..1317)
                     /gene="gldE"
                     /locus_tag="PLI16_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOH84421.1"
                     /translation="MDLPLLYVVVLGTFFKPVTAGIIIGFIFLVVLLACTALVSGSEM
                     AFFSLDAKQLDELKSKKKNHSHLIIQLLKQPQKLLATILITNNFFNVSIVILSTYLTI
                     NLFDLTGHYVLSFLIEFVVLTALILLFGEMLPKIYASQNPLKFASMMAAPMKFLTKIF
                     SPLSFFMVRSTGVIERRLSKKKQVVSFEDLSDAIELTSDEITQQQERKILKGIVKFAD
                     IEVSQVMKPRIDVVAAEYNINFHQLIHFIQNAGFSRIPVYKKTFDHIAGILYIKDLLQ
                     YIGREAAFNWQTLIRPPFYVPENKKINDLLSEFKEKKIHMAVVVDEYGGTSGIVTMED
                     VIEEIVGEISDEYDTESEDIDFTKIDDYTYIFDAKTSLNDLAKKINFSDEFFSDIKGE
                     SESIAGLILEMTGKIPQKNETINIKDLSFTILSSDIRRIKKVKVIIT"
     gene            complement(1343..1771)
                     /locus_tag="PLI16_07400"
     CDS             complement(1343..1771)
                     /locus_tag="PLI16_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOH84422.1"
                     /translation="MAGVNKVILIGNLGKDPEIRQLENGTKVASFSIATTESYKNKDG
                     NKVEHTEWHNIVLWRGLAEIAEKYLRKGNTIYLEGKIRSRSWDDKEGNKRYTSEIIGD
                     NMTMIGGRKDADGAVPQGPGDKAPEQPDIVPGGEGDDLPF"
     gene            complement(1828..3303)
                     /gene="proS"
                     /locus_tag="PLI16_07405"
     CDS             complement(1828..3303)
                     /gene="proS"
                     /locus_tag="PLI16_07405"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOH84423.1"
                     /translation="MAKDFVTKEENYSQWYNDLVIKADLAENSAVRGCMIIKPYGYAI
                     WEKVQAALDHMFKETGHYNMYFPIFIPKSFFSKEASHVEGFAKECAVVTHYRLKNAAD
                     GKGIVVDEDARLEEELIVRPTSETIIWDAYRNWIKSYRDLPILANQWANVVRWEMRTR
                     LFLRTTEFLWQEGHTAHATEQEAMKEAEQMLDVYANFAENYMAMPVIKGIKSANERFA
                     GALETFCVEALMHDGKALQAGTSHFLGQNFAKAFDVKFLNRENKLEYVWATSWGVSTR
                     LMGALVMTHSDNNGLVLPPKLAPIQVAIVPIFKTPEQLSQISERALGIKKALEKRGIS
                     VKFDDRDTLTPGWKFAEYEFKGVPVRIAIGPRDMENGTVEVARRDTLEKSVFQLTDID
                     SKIANLLEQIQKNLFQKAQTYTDDNTYHANTWEEFNDILETKGGFIYAHWDGTPETEQ
                     KIKEETKATIRCIPLNNPLEEGKCVYSGKPSQQRVIFARAY"
     gene            3398..>3607
                     /locus_tag="PLI16_07410"
     CDS             3398..>3607
                     /locus_tag="PLI16_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84424.1"
                     /translation="MKTLVKILFLAASTALFVSCRTTNQTSTVYDDVYGKNPAVVATP
                     GVDQPARTVPPSNTGVTNNEDNGNVI"
BASE COUNT          949 a          801 c          733 g         1124 t
ORIGIN      
        1 tgtaatgata actttaactt ttttaatacg cctgatgtct gatgacaaaa tcgtgaacga
       61 caggtcttta atgtttattg tttcgttttt ttgaggaatt ttgcctgtca tttccaatat
      121 aagtccggca atggattccg attccccttt gatgtcgctg aaaaactcgt cgctgaagtt
      181 tatctttttt gccaaatcgt tcagcgaagt ttttgcgtcg aaaatataag tatagtcatc
      241 aattttggtg aaatcaatgt cttcggattc tgtatcgtat tcatcactga tttcacctac
      301 aatttcttca atcacgtctt ccatggttac tatccccgag gtgccgccat attcgtccac
      361 caccaccgcc atgtggattt tcttttcttt aaactcgctg agcaggtcgt ttattttttt
      421 gttttccggc acataaaatg gcgggcggat aagtgtctgc cagttaaagg cagcctccct
      481 gccgatatat tgcagcaagt ctttgatgta taaaatgccg gctatgtggt cgaaggtttt
      541 tttatacaca ggaattcgtg aaaacccggc gttttgtata aaatgaatta actgatgaaa
      601 attgatgttg tattcggcgg caaccacatc aattcggggt ttcataacct gcgatacttc
      661 aatgtcggca aatttcacta tccctttcag tatcttgcgc tcctgttgct gggtaatttc
      721 gtcagaggtc aactcaatag catccgaaag gtcttcaaag gataccacct gtttcttttt
      781 ggaaagtctt ctttcaatta ccccggttga ccttaccata aaaaaactca gcgggctgaa
      841 tatctttgtc aaaaatttca tcggagcggc catcatgctg gcaaatttca aaggattttg
      901 gctggcataa atctttggca acatctcgcc aaaaagtagt atcagagctg ttaatactac
      961 aaattcaata aggaagctta atacgtaatg tcccgtcaga tcgaagaggt tgatggtaag
     1021 ataggttgac aggataacaa tggatacatt gaaaaaattg ttggtaatca gtatagttgc
     1081 caacaatttc tgaggctgtt tcaaaagttg gatgattagg tgactgtggt tttttttctt
     1141 gctttttagc tcatcaagtt gtttggcatc gagcgagaaa aaagccatct ccgatcccga
     1201 aaccagtgcg gtacacgcca gtaataccac aagaaaaata aagccaataa ttattcctgc
     1261 cgtaacgggc ttaaaaaagg ttccaagcac cacaacatat agtaagggca aatccatagc
     1321 ggattttcag atataaattt aattaaaagg gcaaatcgtc gccttcccca ccgggcacaa
     1381 tatccggctg ttcgggagct ttgtcaccgg ggccttgggg aaccgcgccg tcagcgtctt
     1441 tgcggccacc tatcatggtc atgttgtcgc cgataatttc agaagtgtat cttttgttgc
     1501 cttccttgtc atcccatgac cgcgaccgga tttttccctc cagataaatg gtattgcctt
     1561 ttctgaggta tttctctgca atttcggcta agccgcgcca aagcactatg ttgtgccatt
     1621 cggtatgttc aaccttattg ccatctttat ttttatatga ttcggtggtg gctatcgaaa
     1681 aacttgccac tttggtcccg ttttccagtt gcctgatttc ggggtctttc ccgagatttc
     1741 ctataagtat aactttgttt actcctgcca tgttttttat ttattataaa ttaataaagc
     1801 aaatgtaatt aaaaaaactt aaaaacatca gtatgccctg gcaaaaatta ctctttgttg
     1861 tgagggtttg cccgaataca cgcatttacc ttcttccaac gggttattaa gagggataca
     1921 ccgtatggtg gccttggttt cttcttttat tttttgctct gtttccggcg tgccgtccca
     1981 atgtgcgtaa ataaagccgc ctttggtttc cagaatatcg ttaaattctt cccaggtgtt
     2041 tgcgtgataa gtgttgtcat cagtataggt ttgggctttt tgaaacaggt tcttttggat
     2101 ttgttccagg aggttggcta tcttgctgtc aatgtcggta agttgaaaca ccgatttttc
     2161 caacgtgtcg cggcgagcca cttccacggt gccgttttcc atatctctgg ggccaatggc
     2221 aatacgcacc ggcacccctt tgaattcgta ttctgcgaat ttccagcctg gggtcagggt
     2281 gtccctgtcg tcgaatttta ccgaaatacc tcttttttcg agggcttttt ttatgcccag
     2341 ggctctttcg cttatttgcg aaagctgttc gggtgtttta aaaattggta cgatagctac
     2401 ctgtattggc gccagtttgg gaggcagcac cagcccgtta ttatccgaat gagtcataac
     2461 cagtgccccc atcaggcggg tagaaacgcc ccacgaagtg gcccatacat attccagttt
     2521 gttttctcta tttaaaaact tcacatcaaa tgctttggca aaattctgtc ccagaaagtg
     2581 ggaggtgccg gcctgcaatg ctttgccatc gtgcatcagc gcttctacac aaaaagtttc
     2641 cagcgccccg gcaaagcgtt catttgctga ttttataccc ttaattaccg gcatagccat
     2701 ataattttcg gcaaaatttg catacacatc aagcatctgt tctgcttctt tcatcgcttc
     2761 ctgctccgtg gcatgggcgg tatgaccttc ctgccacaaa aattccgtag tccgcaaaaa
     2821 caaacgggta cgcatttccc aacgcaccac attagcccac tggttagcca atattggcag
     2881 atcgcggtac gattttatcc agttgcggta ggcatcccaa ataatagttt cggaggtagg
     2941 ccttacaata agctcttctt ccagcctggc gtcttcgtca accacaatgc ctttgccgtc
     3001 agccgcgttt tttaaacggt aatgcgttac cacagcgcat tctttggcaa aaccctccac
     3061 atgacttgcc tctttgctga aaaaagattt tggaatgaat attggaaaat acatgttgta
     3121 atgaccggtt tctttaaaca tatgatccaa cgcagcctga accttttccc agatggcata
     3181 tccgtatggt ttgataatca tacacccacg cacggcagaa ttctctgcaa gatcggcctt
     3241 aattaccaaa tcattgtacc attgcgaata attttcttct tttgtgacaa aatctttagc
     3301 catagttaaa agtggtataa aatttgtgtt taataaaaaa acgaagcaaa actaattaaa
     3361 aaagtaaaat aacactataa aattcggagg ttaaatcatg aaaacacttg ttaaaattct
     3421 cttcctggct gcaagcacgg cattgtttgt atcctgcagg accaccaatc agacaagtac
     3481 tgtatatgat gatgtatatg gaaaaaaccc ggccgttgtt gccactcccg gagtggatca
     3541 gccggccaga accgttcccc caagtaacac aggggtaaca aataacgagg ataacggtaa
     3601 tgtgata
//
LOCUS       DAOSFP010000237         3606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23875_length_3606_cov_3.102788, whole genome shotgun sequence.
ACCESSION   DAOSFP010000237 DAOSFP010000000
VERSION     DAOSFP010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23875_length_3606_cov_3.102788"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PLI16_07415"
     CDS             <1..424
                     /locus_tag="PLI16_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84425.1"
                     /translation="RTSPVSRFQKFQYRLLYSNYYFGLILYHARKLIKSTTARHHRMA
                     KPAKQAIFSAYGAFFIFHRSYFEMGGTIDDGYFLYGEEDSVAAQCAENGMKIALLPDF
                     EVFHNEHISTDSTGFKKNIYQLQQKAYRYIKANYLNFY"
     gene            424..1467
                     /locus_tag="PLI16_07420"
     CDS             424..1467
                     /locus_tag="PLI16_07420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH84426.1"
                     /translation="MTEKTIISILIPCYNEEKFIGKCLDSVRAFEIPDGCETEVLVID
                     GMSADKTREIVAAKIMEDPGIRLIDNPGRIQSYALNTAIPLSKGSYILRLDSHSDYPV
                     NYLKLLYETALRTGADNTGGLVITRPFNKSYRASVVQALTTHKFGVGNSGFRVGMKEG
                     PADTVPYGFFKRSIFNKIGYFDERLVRAQDYEFNRRIWRAGGTIWLNPEVQLNYYNQP
                     DMHKFLKKQLLYEAPYNAYMWFVAPYTFAYRHAITAVFFGGIIGGMLLSLFVPVLWYV
                     LAAVMAVYFLLAIISSVQQAVRYKKFLHVFTLPVCFFLYHFLHGSGVVWGLLRLLTRT
                     APIFKKPKTQATK"
     gene            1490..2269
                     /locus_tag="PLI16_07425"
     CDS             1490..2269
                     /locus_tag="PLI16_07425"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOH84427.1"
                     /translation="MNDKTKIETEHYDQKAAVRGDDPDKIGKSRVDMALQNAHDCFIA
                     QIKTLQAEKKSGLILDYGCGTGYKTMFLADDNWKIMGIDISSVSIEFANKAVQKNNAN
                     AMYQVMDCEQMQFSDNSFDLVIDYGTFSSLSMEKAFPELLRVLKPDGSLVAIETFGHN
                     PLTNFKRWLNVRSGRCTKWAASHIMKKDDWKKMAGSFSECTMYYRGFLVLMLVPVFSI
                     CPRWLMRSVEAADRLFLKIPFFKYFAFKTVVVLKNPKKPIM"
     gene            2266..2862
                     /locus_tag="PLI16_07430"
     CDS             2266..2862
                     /locus_tag="PLI16_07430"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOH84428.1"
                     /translation="MKRIFDFIFSLSVLLIFSPLLIILSLIIYFNDFGPVFYMAPRVG
                     QNGRLFRMFKFRTMVVNADKIGASSTTASDKRITRVGKLLRKTKLDELPQFINVLTGQ
                     MSVVGPRPEVKSFTDMFTEEEKAILSVKPGITDYASIWNSDEGKILEGAEDPDMAYKE
                     LIWPEKKRLQLKYVREHSFWTDIKIVFLTLLIIVRKYT"
BASE COUNT         1092 a          656 c          776 g         1082 t
ORIGIN      
        1 caggacaagc cctgtgagca ggtttcagaa atttcaatac cgtttacttt attccaatta
       61 ttatttcggc ctgattcttt atcatgccag aaaactaatc aaatcaacta cagcaaggca
      121 tcatagaatg gcaaaaccgg cgaaacaggc catcttttct gcttatgggg ctttctttat
      181 ttttcaccga agctattttg aaatgggcgg tacaattgat gacggatatt tcctttatgg
      241 agaagaagac agcgtggctg cgcaatgtgc tgaaaacggc atgaaaatag cgttattgcc
      301 tgattttgaa gtgtttcaca atgagcatat ttccactgat agcacaggat ttaagaaaaa
      361 tatctaccaa ttgcagcaaa aagcctacag gtatattaaa gctaactatc tcaattttta
      421 ttgatgaccg aaaaaacaat aatatcgatt ctgattccct gctacaacga agagaagttt
      481 attggcaaat gcctcgatag tgtacgcgct tttgaaattc ctgacggatg cgaaaccgaa
      541 gtattggtaa ttgatggcat gtcagcggat aagacacgtg aaattgttgc ggccaaaatc
      601 atggaagatc caggcatccg cctgattgat aaccccggaa ggatacagtc gtatgcacta
      661 aatacagcca taccgctatc aaaaggttca tacatcttaa gactggattc tcattcggat
      721 tatccggtca attatctgaa gctgctctac gaaacggctc tgcgcaccgg cgccgacaac
      781 accggcgggc tggtgattac caggcctttt aataaaagtt atagggccag tgttgttcag
      841 gcgctgacca cccacaaatt cggcgtgggc aattctggtt tcagggtggg catgaaagaa
      901 ggccccgcag atacggtgcc ttatggtttt tttaagcgca gtatttttaa taagataggc
      961 tattttgatg aacggcttgt ccgggcgcag gactatgagt tcaacaggcg gatatggagg
     1021 gcaggaggga ccatctggct taatcccgaa gtgcagctga attattacaa ccagccggat
     1081 atgcacaaat ttttaaaaaa acaattgctg tacgaagcgc cttacaatgc ctacatgtgg
     1141 tttgtggcac cttatacttt tgcatacagg catgccataa cggctgtgtt ttttggtggg
     1201 attatcgggg gcatgctgtt gtctctcttt gtacctgttc tgtggtatgt tcttgccgct
     1261 gttatggctg tatatttttt gcttgctatc atatcatcgg tgcagcaggc tgtgaggtac
     1321 aaaaaattcc tccatgtttt taccttgcct gtgtgttttt tcctatatca ttttttgcat
     1381 ggttcagggg ttgtatgggg tttgctccgc ctgttgacca ggaccgcgcc aatatttaaa
     1441 aaacctaaaa cccaggcaac gaagtaatca tttgattatt aacgcaccta tgaatgacaa
     1501 aacaaaaata gaaaccgaac attatgacca gaaagcggct gtaaggggag acgatcctga
     1561 taaaatcggg aaaagccgag ttgacatggc tttacaaaat gctcacgatt gttttattgc
     1621 acaaataaaa acattgcagg cagagaaaaa atcaggatta atacttgatt atggctgtgg
     1681 tacaggttac aaaaccatgt ttctggccga tgacaactgg aagattatgg gcattgatat
     1741 ttcatccgtt tccatagaat ttgccaataa agctgtacaa aaaaacaatg ctaatgccat
     1801 gtatcaggtt atggattgtg agcagatgca attttctgat aactctttcg acctggtaat
     1861 tgattacgga actttttcca gcctcagcat ggaaaaggct tttcctgaac tgctgagggt
     1921 attaaagccc gacggaagcc tggtggcaat tgaaaccttt gggcacaatc cgttgaccaa
     1981 ctttaaaagg tggcttaatg tgaggtcggg aagatgtaca aaatgggcag caagtcatat
     2041 tatgaaaaaa gatgattgga aaaaaatggc cggcagtttt tctgaatgta cgatgtatta
     2101 ccggggtttt ttggtgctga tgctggtgcc tgttttttct atttgtccgc gatggctaat
     2161 gcgcagtgtt gaagctgctg accgcctgtt tctcaaaata ccttttttta aatactttgc
     2221 attcaaaacc gttgttgttc ttaaaaaccc caaaaaacca ataatgtgaa aagaattttc
     2281 gattttattt ttagccttag cgtactgctc attttttcac cacttctgat tattttatca
     2341 ttgataattt atttcaacga ttttggcccc gtgttttata tggcgccgcg tgtgggacaa
     2401 aacggcaggc ttttcaggat gttcaagttc agaaccatgg ttgtcaatgc cgacaagata
     2461 ggggcatcat ctaccacggc ctccgataag cgtattacga gggttggaaa gcttctgcgc
     2521 aaaaccaagc ttgacgaact tccgcagttc atcaatgttt tgacaggcca aatgagtgta
     2581 gtgggcccgc gtcccgaagt aaaatcattt accgatatgt tcaccgaaga agaaaaagcc
     2641 atattgtcgg tcaagccggg cattaccgat tatgcgtcta tctggaactc cgacgagggg
     2701 aaaattctgg aaggcgccga agaccccgat atggcatata aggagctgat atggcctgag
     2761 aaaaaacgtc tacagcttaa atatgtaagg gagcattctt tttggacaga tattaaaatt
     2821 gtttttttaa ctttgctgat tattgtaaga aagtacacat gaaaaattct gaatcagact
     2881 aagtctgaga atgtgcttga atttatggaa cggattaatt gttgagttta attgagattg
     2941 tcagagcaaa cgctctaatt ggcttgatag attttacttt ctttttgtca cgttatgaaa
     3001 aagtcattcg ttgggatata ttgtaggtta aacatataat agttaataat aggtgtattt
     3061 taagatttta tattttgaaa aatattgaat tatggatttg gtttaagaaa accattaacg
     3121 ggatcgaacg atttttttga atcaaaaaat taatatgcat tttgagaacc taccattagt
     3181 tctgaaatct aattatattt ttaataatca atcaagttca tatcatatga aaaaaattac
     3241 tcttttattg ttttgcataa tcgttttggc tttttgcagt tgcgagaaat caaaatcact
     3301 cattgaaagc gaagaacaat cgaacctttt tgccttaaac aaaagtacga gaacttgtcc
     3361 aaattgtaga aattggagtg ctataactgt aacgaattca acaatgttga aaacaacaat
     3421 tgaaaatgct gaatcctgca ccattattaa tttgccagct ggtaactata atgtttctag
     3481 tgaaatatat atttcgaaga aatgccattt aaagataagg ggagctggaa tgggtcgcac
     3541 tgttttattg ctaacacata gagcgatagc cgaaaagccc taaaacgaaa gcccgaaaga
     3601 attgat
//
LOCUS       DAOSFP010000238         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_23942_length_3599_cov_3.255643, whole genome shotgun sequence.
ACCESSION   DAOSFP010000238 DAOSFP010000000
VERSION     DAOSFP010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23942_length_3599_cov_3.255643"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLI16_07435"
     CDS             <1..433
                     /locus_tag="PLI16_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOH84429.1"
                     /translation="ALSKDFSEKYLQVLPTKNKVFPGTYETLDYLGGRYLLHIVTNGF
                     EEVQYRKIHYAGLEKYFDRVITSEKAGCKKPDRMFFEYTLKEINAEARDCLMIGDDME
                     VDLAGSRRAGIDQVFCNFENISHVESFTFEISQINDLRTFL"
     gene            complement(454..1209)
                     /locus_tag="PLI16_07440"
     CDS             complement(454..1209)
                     /locus_tag="PLI16_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84430.1"
                     /translation="MLTSVEAQHCPRNLYDCKGACGWFTDSDNDGYCDLTAFSEAIIK
                     KSLHKKDSIENAQQQKNPNVTDSLPQTANKTSTNNVGTQKNTGNKKLSEPVSDHKCPF
                     ANTPECEKNQVEQTNVSGTDKGQQVQNDDKNKNFEIKKYDIFLIFGLSILCYLISWIL
                     SKKNVWKKSTHKKFWNTILLITFLNTGLTGLFLVIQINYLVLFDWFSKLLFWHVEFGI
                     SMAAISIVHILWHWKYFWHLLVKRKDTSDCKEA"
     gene            complement(1356..2291)
                     /gene="miaA"
                     /locus_tag="PLI16_07445"
     CDS             complement(1356..2291)
                     /gene="miaA"
                     /locus_tag="PLI16_07445"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOH84431.1"
                     /translation="MGIQQFSPGQKHLIIILGPTAVGKTSLAINLAQHFKTEIISADS
                     RQFYREMTIGTAKPSPEELALVRHHLVGMLSISDYYNVFRFETEVMSLLENLFTMHHV
                     VIMAGGSGLYIDTICHGIDHIPDPDEDLRNELNQIFLQQGIQPLRDRLKLLDYQAYQK
                     IDLANPKRIIRALEVCLTTGRAYSSFLNNVNPDRPFNIIKIGLKRERKELYDRINRRV
                     DDMVANGLAEEAHTLYPYRDYNSLQTVGYRELFEYFDGSYPLETAIEKIKTNTRRYAK
                     KQMTWFARDKDIHWFSPDEAESIKTFLTKQTNTLY"
     gene            complement(2299..2886)
                     /locus_tag="PLI16_07450"
     CDS             complement(2299..2886)
                     /locus_tag="PLI16_07450"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84432.1"
                     /translation="MLIYRFKVINDEVDNFYREIEIKPGNTFEDFHKLLVECINLTPG
                     EMASFYICDSRWNKLQEISLCDLSEEQDSGEEPANDVKPVKIMADVTLKQSINDPNQR
                     LIYVYDFLKMHTFYIELSKITEGDPKNTYPRCTKSVNDIVKPKKTPSKAADIIEKEEE
                     ELIDEEADNFFDDDDLPADDGSIDLNDGFDDSKYL"
BASE COUNT          985 a          686 c          786 g         1142 t
ORIGIN      
        1 tgccttgtcg aaagactttt ctgaaaaata tctgcaagtg ttgcctacta aaaacaaagt
       61 gttcccggga acctatgaaa cgctggatta tcttggcggc aggtatttac ttcatattgt
      121 aaccaacggt tttgaagaag tacagtatcg aaaaattcac tatgcaggac tggagaaata
      181 ttttgacagg gtgattactt ccgaaaaagc cggctgtaag aaacccgaca ggatgttttt
      241 tgaatacacc ctgaaagaaa ttaatgccga agccagggac tgcctgatga taggcgatga
      301 tatggaagtg gacctggcag gttcacgccg ggccggtatc gatcaggtgt tctgtaactt
      361 tgagaacata agccacgtcg aaagttttac ttttgaaatc tcgcagatca acgacctgag
      421 aactttctta taatcttcgc tacaagctgc tttttatgcc tctttgcagt ccgaagtgtc
      481 tttgcgtttt accaacaggt gccagaaata tttccaatgc catagaatat gaacgatgga
      541 tatggcagcc atgctgatgc caaattccac gtgccagaaa agaagttttg aaaaccagtc
      601 gaataataca aggtaattga tctgtattac caggaaaagg ccggtgaggc cggtattcag
      661 aaaggttatc aataatatgg tgttccagaa ttttttgtgg gtgctttttt tccaaacatt
      721 ttttttcgag agtatccatg atatcaggta acacaaaatg cttaatccga agatcaggaa
      781 gatgtcgtat ttttttattt caaaattctt gtttttatca tcattctgca cctgttgccc
      841 tttatcagtc ccgcttacat tggtttgctc aacctgattc ttttcacatt cgggggtgtt
      901 ggcaaaaggg catttgtggt cggagacagg ttcagacaat tttttgttac cggtattttt
      961 ctgcgtcccg acattgttgg tgcttgtttt gtttgctgtc tgcggcaggc tgtcggtaac
     1021 atttggattc ttctgttgct gggcattttc gatagaatct tttttgtgca aacttttttt
     1081 tataatcgct tcgctgaacg ctgtaaggtc gcaatagccg tcgttgtccg aatcggtgaa
     1141 ccagccgcag gcgcctttgc agtcatataa atttcttgga caatgttgtg cttcaactga
     1201 agttaacaag gtgaatgtca gtaatataat tgtcaatatg cgagtcataa tgcaaaaatt
     1261 gaagttattt tgttttacaa aacgtgagtc aggtaaaatt cttataaact ttttgaaaat
     1321 gggtgcaaaa atacgaaata atctgtcaaa ataactcaat aaagggtatt agtttgctta
     1381 gtaagaaatg ttttaatact ttctgcttca tcgggcgaaa accaatgaat atctttatca
     1441 cgggcaaacc aggtcatttg tttcttcgcg tacctgcggg tgttggtttt gattttttct
     1501 atggccgttt ccagtggata gctgccgtca aaatattcaa agagttctct gtatcctact
     1561 gtctgcaggc tgttgtaatc ccggtaagga taaagggtgt gggcttcttc ggccaggccg
     1621 ttggctacca tatcatccac acgccggtta attctgtcgt ataattcttt gcgttcgcgt
     1681 ttcagaccaa tttttatgat gttgaatggg cggtcagggt ttacattatt taaaaatgaa
     1741 gagtaggctc ttcctgtagt taaacatact tccaaagccc tgattattct cttggggttg
     1801 gccaggtcaa ttttctggta tgcttggtaa tcaagcagtt taagccggtc gcgtaagggt
     1861 tgaatgccct gttgcagaaa gatttggttg agttcgttgc gcaaatcctc atccggatcg
     1921 ggaatgtggt caatgccgtg gcatattgta tctatataca ggcctgaccc accggccatg
     1981 atgactacgt ggtgcatggt gaaaagattt tccagaagac tcattacctc ggtttcaaag
     2041 cggaaaacat tgtagtagtc cgaaatggag agcatgccga ccagatgatg ccttacgagg
     2101 gctaattctt caggcgaagg tttggcagtg ccaatagtca tttcgcgata aaactgccgc
     2161 gaatcggcag agattatttc tgttttgaag tgctgtgcca ggttaatggc aagagaagtt
     2221 tttcccacgg cggtgggacc cagtatgatg atcaggtgtt tttgaccggg agaaaactgt
     2281 tgaataccca tctattattt aaaggtattt tgaatcatcg aacccgtcgt tgaggtcaat
     2341 actgccatca tcagcaggga ggtcgtcgtc atcaaagaaa ttgtcggcct cttcgtcaat
     2401 taactcttcc tcttcttttt caatgatatc agcagctttg gacggtgtct tttttggttt
     2461 gacaatgtcg ttgaccgatt ttgtgcaacg gggataggtg tttttggggt caccttcggt
     2521 aattttcgat aactcgatat agaaagtatg cattttcagg aagtcgtaaa catagatcag
     2581 gcgctggttg gggtcgttaa tactttgttt cagcgtaaca tccgccatta tttttaccgg
     2641 ctttacatcg tttgcaggtt cttcgccgga atcctgttct tccgataagt cgcagagcga
     2701 tatttcctgc agtttgttcc acctgctgtc gcaaatataa aaagaggcca tttcgcccgg
     2761 tgtaaggtta atgcattcca ccaaaagctt atgaaaatcc tcgaaagtat tgcctggttt
     2821 aatttcaatc tccctgtaaa aattgtcaac ttcgtcgttg ataactttaa atctgtatat
     2881 cagcatttct ctattatgtt tctctattat gtttctataa aattagtgca aaattaaaca
     2941 aaaagcaata ataatattga aaaaataaat atcgtgacca atcatttttt gaggtgttgg
     3001 cggagattat ttcattgtaa ataacttaac ttttgcattt cagataaaaa tgagaaactc
     3061 ttacattttt aataagttgt tcattgttac taatgctgag actcgtgtgt tgccattcag
     3121 ggaaagtaga tgtgtatgta actttttttc ggaaaaaaag ttaccaaaaa accttgtcaa
     3181 aacgaatgct tccgcgcgct ctcatcaggt cgggaaaatc atgcaaacga gtgcccgaaa
     3241 tagccttcaa aggacggtaa cctgttgcct gatttctttt gtgagaaaaa gtgtttgtgt
     3301 taatcggcgt aaagtgcagg ccatttcaga caaacagccc ggttagccgg gctgcctttg
     3361 ctgattttca ccttcctgct gattcacgcc agcgccaccc cgtcgttttg acgggccaac
     3421 gcgccgcaat taacaaacat ttgtaatatg cgaatacatt caaatggatt taaaactaca
     3481 tcctaaagtg tcatctgcgg cggaaaaatt gcgaaatatt caaaagcaaa aaaagaggct
     3541 gtttcactaa aacagcctct tgatatttat cactaagaag atttattaat ggacaaagt
//
LOCUS       DAOSFP010000239         3584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24097_length_3584_cov_3.900255, whole genome shotgun sequence.
ACCESSION   DAOSFP010000239 DAOSFP010000000
VERSION     DAOSFP010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24097_length_3584_cov_3.900255"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /gene="purH"
                     /locus_tag="PLI16_07455"
     CDS             complement(<1..1512)
                     /gene="purH"
                     /locus_tag="PLI16_07455"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOH84433.1"
                     /translation="MIKINSALVSVYHKTGLDEIIESLNVLGVKIYSTGGTLDYIRKY
                     CHDAVSVESVTDYPALFGGRVKTLHPKVMGGILYQRNNAQDVQQAKDFAIPSIDLVIV
                     DLYPFEKTLAGGASEEEIIEKIDIGGISLIRAAAKNFNDVVIVPSADLYGKLAQLLKE
                     KNGYTSLEDRKYFAAQAFNVSSHYDTAIFNYFNAAEKIPVFKQSCSQSTLLRYGENPH
                     QQGMFFGDLNEVFEQLHGKSISYNNLVDIEAAVNLMADFEEATFAILKHTNVCGLACR
                     AQLAEAWKDALAGDPVSAYGGVLITNRTVDKETATAVNEIFFEVLIAPDYVPEALEIL
                     FAKKNRIILKQKKFAFAAQQYKSLLNGVIVQDKDLKTETSEDLRVVTDTAPAAAEIDD
                     LLLANKIVKHTKSNTIVLVKNRQLLGNGTGQTSRVDALRQAIIKAREFGKDLNKAVMA
                     SDAFFPFADCVEIAAQAGISAVIQPGGSVRDSDSISCCNQHKIAMVFTGYRHFK"
     gene            1769..3019
                     /locus_tag="PLI16_07460"
     CDS             1769..3019
                     /locus_tag="PLI16_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH84434.1"
                     /translation="MILFIRLLRESYLFAFQSIIANKLRTFLSLLGITIGIFAVTTVF
                     TVVDSMEITIRKNIESLGNNVLFIQKWPWSFSQDFPWWKYFNRPQPRYSEMNELVKRS
                     SSIEVATFMISTSKTIKHQDKSIDNIMIAGASHDYEKVFTIKLGSGRYFTPQESAGGH
                     PVVIIGSNVAENLFGNTDPLGQDVKIFGRKVQVIGVFEKEGTDTFGNTADDQALIPIN
                     YARNIIDINNEYYDPTLIVKAKPNISNAQAKDELTGIMRSIRKLKPGSDDDFSINEPD
                     LLTKGFENIFNVISIAGWIIGGFSLLVGGFGIANIMFVSVRERTNIIGIQKSLGAKNY
                     FILFEFLFEAIMLCIVGGLFGLLLVYGGTLIVSAAFDMELMLTADNIILGLTVSAFIG
                     LISGYVPAYMASKLDPVEAIRAGN"
     gene            complement(3049..>3584)
                     /locus_tag="PLI16_07465"
     CDS             complement(3049..>3584)
                     /locus_tag="PLI16_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84435.1"
                     /translation="SAAPTYFETASIKSVYGAPYAMIDGGVFANNPALCAYAEARKMK
                     FSEILNDAKKPDLPMAKDMIIVSIGTGAKGKPYLYKDARKWGAVGWLLPIIDILMSGN
                     AETVSYQLKQIFKTLQKEEDRNDYYRLEPGIGEAKSAMDCADEYNIKKLVESGKRFVT
                     EHEEELNGIVARLIANK"
BASE COUNT          952 a          790 c          775 g         1067 t
ORIGIN      
        1 tttaaaatgt ctgtatcctg taaaaaccat agctatttta tgttggttgc agcaacttat
       61 ggaatcacta tcccgcaccg agcctccggg ctggattacg gcactgattc ctgcctgtgc
      121 ggcaatctca acacagtcgg caaaaggaaa gaaagcatcg gatgccatca cggccttgtt
      181 gaggtcttta ccaaactcac gggcttttat aattgcctgg cgtaaggcat ccacccggga
      241 agtctgcccg gtaccattac ccaacagctg acggttttta accaaaacaa ttgtgttgga
      301 cttggtgtgt tttactattt tgtttgctaa caacaagtca tcgatttcgg cggcggccgg
      361 agcggtatcg gtaaccaccc ggagatcctc actggtttct gtcttcaggt ctttgtcctg
      421 cacgatgaca ccgttcagca gggatttgta ttgttgggcc gcaaacgcaa acttcttttg
      481 tttcaggatg atcctgttct ttttggcaaa aaggatttca agagcttccg gcacataatc
      541 cggcgcaatc agcacctcaa aaaatatctc attaacggct gtggccgttt ctttgtcaac
      601 agttctgttg gttatcagca ctccgccata ggctgacacg gggtctcctg ccagggcatc
      661 tttccatgct tcggccagtt gtgcacggca agccagaccg cagacattgg tatgttttaa
      721 aatagcaaaa gttgcctcct caaagtcggc cataagatta acggcagctt ctatatccac
      781 aaggttattg taggaaatgc tttttccgtg caactgctcg aacacttcat tcaggtcgcc
      841 aaagaacatg ccctgctggt gaggattttc cccatagcgc aggagggttg attgactaca
      901 gctctgcttg aaaacaggaa ttttttcggc ggcattaaaa tagttaaaaa tagccgtatc
      961 ataatgcgac gacacattaa aggcttgagc ggcaaaatat ttacgatcct caagagaggt
     1021 atagccattt ttttctttca gcaattgtgc aagtttgcca taaaggtcag cagaaggaac
     1081 aataaccaca tcattgaagt tttttgccgc ggcccgaatc agcgaaatgc cgccgatatc
     1141 aattttttca atgatttctt cttcggaagc acccccggca agcgtttttt caaatggata
     1201 caaatccacg atgaccagat caatggaggg tattgcaaaa tcttttgcct gttgtacatc
     1261 ctgcgcgttg tttcgctggt aaagaattcc acccataacc ttgggatgca gggtttttac
     1321 acgcccgcca aaaagtgccg gataatctgt taccgattcc acagaaactg catcatgaca
     1381 atatttcctg atataatcaa gggtgcctcc cgttgaataa atctttacgc ctaaaacgtt
     1441 aagactctcg ataatctcat caagtcctgt tttatgatat actgaaacta aggcactgtt
     1501 aatcttaatc ataggtccgg tgggtaaatt tttctgcaaa ggtcggtaaa tttagttaat
     1561 aaaattgttt tactgtcaaa ttaatatgga ttgatttttc taggctgttt tcagtatcca
     1621 ggcaaaattc tgtattgttc tacgatagtt ttttttccgg tcccaaacaa ttttgtaatt
     1681 ttatggcatg tttgcctaat ggccatctta ttaaatttac aatttaaacg attaaacgca
     1741 aacatagtta cttttttaac gaaaaaaaat gatacttttt ataaggctgc tacgcgaaag
     1801 ttatttgttt gcttttcaat ctattatcgc aaataaattg agaacatttt tgtcgctgtt
     1861 aggtataaca atagggattt ttgccgtaac aacggtgttt acggtggtgg attcgatgga
     1921 aattaccatt cggaaaaaca ttgaatccct gggcaacaat gtgcttttta ttcaaaaatg
     1981 gccatggagt ttcagccagg attttccctg gtggaaatat tttaaccggc cgcaacccag
     2041 gtattccgag atgaatgagc tggtgaaacg ttccagcagc attgaagtgg ccacttttat
     2101 gatttctacc agcaaaacca tcaaacacca ggacaaaagc attgacaata tcatgattgc
     2161 gggtgcatcg cacgactacg aaaaggtttt taccatcaag ctgggcagtg gacgttattt
     2221 caccccacag gaatcagccg gtgggcatcc ggtggttatc atcggcagca atgtggctga
     2281 aaaccttttt ggcaataccg atcccctggg ccaggatgta aagatttttg gcagaaaggt
     2341 tcaggtcatc ggtgtttttg agaaggaagg cactgatacc tttggcaaca ccgccgacga
     2401 tcaggcattg atacccatca attacgcacg aaacattata gacatcaaca acgaatatta
     2461 cgacccgaca cttattgtta aagccaaacc aaatatttcc aacgcacaag ccaaagatga
     2521 actgacgggt attatgcgtt cgatacgcaa gcttaagccg ggcagcgacg atgacttttc
     2581 tattaacgag ccggatttgc tcacgaaggg tttcgaaaat attttcaacg ttatttccat
     2641 tgcaggatgg attatcgggg ggttttcgct gctggttggg ggttttggta ttgccaatat
     2701 tatgtttgta tcggtgcgcg aacggaccaa cattattggg atacaaaagt cgctgggagc
     2761 caaaaattat tttatacttt ttgaattttt gttcgaagcc attatgcttt gtatagtcgg
     2821 cggcctgttc ggactgttgc tggtatatgg cggcacactg atagtgtcgg cagccttcga
     2881 catggaactg atgcttaccg cagataatat cattcttgga ttaacagtgt cggcctttat
     2941 cgggttgatt tcgggctatg ttcctgccta tatggcctcc aaactcgatc cggtggaagc
     3001 catacgtgcc ggaaattgag tttgccgtgc ccgttttttt tgaatttgct acttattggc
     3061 tatcagcctg gccacaatcc cgttcagctc ttcttcatgc tcggtaacaa accgcttgcc
     3121 ggattccacc aattttttga tattatactc atcggcacaa tccatcgctg atttcgcctc
     3181 cccgatacct ggttcgagcc ggtaataatc attgcggtct tcttcttttt ggagtgtttt
     3241 gaaaatttgt ttcaactgat aacttaccgt ttcggcgtta cccgacatga gaatatcgat
     3301 gataggcagc aaccagccca cggcacccca ttttctggca tctttgtaca gataaggttt
     3361 tccttttgcc ccggtgccaa tagaaactat aatcatgtct ttggccatag gtaaatcagg
     3421 cttttttgca tcgttcaaaa tttcgctgaa tttcattttt ctggcctcag cataggcaca
     3481 aagtgccgga ttattggcaa aaaccccgcc atcgatcata gcatagggcg ctccgtaaac
     3541 cgatttgata gaggctgtct caaaataagt aggagctgcc gaag
//
LOCUS       DAOSFP010000240         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24116_length_3582_cov_3.731783, whole genome shotgun sequence.
ACCESSION   DAOSFP010000240 DAOSFP010000000
VERSION     DAOSFP010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24116_length_3582_cov_3.731783"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="PLI16_07470"
     CDS             complement(<1..1036)
                     /locus_tag="PLI16_07470"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOH84436.1"
                     /translation="MNRIFFLLPALCCMFTFSGLQAQNKRALVIAVGDYNYATTRWRP
                     ISSAQDVPLIKNALLTQGFKEADIRLLQDKDADKAGIVKAFDDLAANTNPGDVVFVHF
                     SGHGQQIEDNDGDEIDGFDEALIPVNARQFFKKDVYEGENHLRDDEFGKLLNTVREKA
                     GPKGNVMVVVDACHSGTSTRGENNTRGTSEVFASPGYKPADNKAAPENYGVYTEDDKL
                     APMACFYGASAHQLNYEYKVNDSTYVGSLSYAFSKAFGESTPLTTYKGLFDRIRVIMA
                     SVAPNQAPQAEGTLDQYILAGKLTGVPDYATVHGFFDEKNVSINRGLLHGVNKGAKVG
                     FYNVDEREYKN"
     gene            complement(1051..2502)
                     /locus_tag="PLI16_07475"
     CDS             complement(1051..2502)
                     /locus_tag="PLI16_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOH84437.1"
                     /translation="MKKFVIFFLITVFGCSGYAQNTSDMLETVLSSVVTVAVYKVEST
                     RQILGFRGNSDIAYDQALDLSNSVASGSGFIIEKGGKKYVITNAHVVEDASTDKGSIY
                     VYSINRSKYEVAILGGDSFYDIAVLTFIDTPAAEVSALPIRTTEARIGETVYAIGNPL
                     GEYPYTISDGIISARNRVRGGATGKFGFLQTTATVIWGNSGGPLVDTKGELVGINSQI
                     AFAQANDMLLWQPQINFALEGLLSNRLIDEIINNDGRVKRAFIGIEVSQRYETVFDRV
                     TSTDQWQLKDMEPVISGVMRSSPAFSVLADKLGARLLEVNGAVVRNIQEVLGEFEKAK
                     PGETVSLLIFHDNAEEKVEIKTQELNARHHEQIARYVMEKEDRVSIVFDDNKVLLNIG
                     KEYANKKVQSKKISTQEDSYQVLTVGLSEEDYDVLWRLNSLSDLGAALRFTGPYGFYD
                     MVVVDQKKPRAKPEKIRISLADSENLQKLTLWY"
     gene            complement(2645..3055)
                     /locus_tag="PLI16_07480"
     CDS             complement(2645..3055)
                     /locus_tag="PLI16_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84438.1"
                     /translation="MKRTVLLSVLMIAFVFATANSASAQKFKDLVKQKVEGTRGANSD
                     LKDKVPCEMPDVVKSKIKKVKGSWCTIQFQNWTGYYIHIWVDGYYQGQLAPWAKSDID
                     VVSGWKDVYCETSGGTYSWVSEGDCDGTHSFNLQ"
     gene            complement(3254..>3582)
                     /locus_tag="PLI16_07485"
     CDS             complement(3254..>3582)
                     /locus_tag="PLI16_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84439.1"
                     /translation="EGSGLGLQNSFELMQELFANLSAGSGAVVISAAAGTGYALESPE
                     WNNGVFTYCILNGLKNLAADASGDKIVSVSELKDYVSKEVERLTNGAQRPTSRKESLE
                     FDWKLW"
BASE COUNT          941 a          882 c          729 g         1030 t
ORIGIN      
        1 catttttata ttcgcgttca tccacattat agaatccgac tttggcgcct ttattcacgc
       61 catgcaataa gcccctgttg atggagacat ttttttcgtc gaaaaaacca tgcaccgttg
      121 catagtcggg tactcccgtt aatttgccgg ccagaatata ctggtcgaga gtgccctcgg
      181 cctgtggcgc ctgattaggg gcaacgcttg ccatgataac acgaatccgg tcgaaaaggc
      241 ctttataggt tgttaatggt gtggattcgc caaaagcctt actaaaagca taggacaggg
      301 agcccacata tgtggagtcg ttcaccttat actcataatt gagctggtgt gctgatgcac
      361 cataaaagca ggccatcgga gccagtttgt catcttcggt ataaacccca taattctccg
      421 gtgccgcctt gttatctgct ggtttgtagc cggggctggc gaaaacctcg gaagtcccgc
      481 gcgtgttgtt ttcccccctg gttgaagttc ctgaatggca ggcatcaact actaccatca
      541 cattgccctt aggcccggct ttttctctaa cggtattcag cagcttgcca aattcatcgt
      601 ctctcaggtg gttttccccc tcgtacacgt cctttttaaa aaattgacgg gcattgacgg
      661 ggatgagcgc ctcgtcgaag ccatcgattt catcaccatc attgtcttcg atttgctgtc
      721 catgccctga aaaatgcaca aaaaccacgt cgccgggatt ggtattggcc gcaagatcgt
      781 cgaaagcttt tacaatcccg gccttatcag cgtctttatc ctgaagcagg cggatatcgg
      841 cctctttaaa gccctgggta aggagtgcat ttttaatcag tggcacatcc tgagccgatg
      901 aaatggggcg ccaccgggtt gttgcgtaat tataatctcc aacagctatc acaagagccc
      961 gtttgttctg ggcctgtagt ccgctgaacg taaacataca gcacaaagcc ggtaacaaaa
     1021 aaaatatcct gttcataaag taggctgttt tcaataccaa agagtcaatt tttgtaaatt
     1081 ttcgctatcg gcaaggctaa tacgaatttt ttcaggcttg gccctgggct ttttttggtc
     1141 cacgactacc atgtcgtaaa agccataagg ccccgtaaag cgaagtgccg caccaagatc
     1201 gctcaaactg ttgagccgcc ataaaacgtc ataatcttct tcacttaatc cgacggtaag
     1261 cacctgatac gaatcttcct gcgtgctgat tttcttcgac tgcacctttt tgttggcata
     1321 ttccttccca atgttgagta acaccttatt gtcatcgaaa acaatactca ccctgtcttc
     1381 tttctccatc acataacggg caatctgttc atgatgccgg gcgttgagtt cctgggtttt
     1441 gatttcaact ttttcttctg cgttgtcatg gaagataagc aacgaaacgg tttctcccgg
     1501 tttggccttt tcaaattcgc caagcacttc ctgtatattt ctcactaccg cgccattgac
     1561 ctcaagcagc ctggcgccaa gcttatcagc caaaacgctg aaagctggag aagaacgcat
     1621 cacgccggaa atcacaggtt ccatatcttt aagctgccat tggtcagtac tggtaacacg
     1681 gtcaaaaaca gtttcgtaac gttgcgacac ttcgataccg ataaaagccc tctttacacg
     1741 accatcgttg ttgatgattt catcaataag cctgttgctg agcaggccct ccaaagcaaa
     1801 attgatttgc ggttgccaga gcagcatatc gtttgcctgg gcaaaggcaa tctgagagtt
     1861 gatacctacc agttcgcctt tggtatctac caacgggccg ccgctgttgc cccagattac
     1921 tgtggcggta gtttgcagaa agccgaattt tcctgtcgct ccgcccctga cacggttcct
     1981 ggcgctgata attccgtcag aaattgtata gggatattcg ccaagcggat tgccaatggc
     2041 gtaaactgtc tctccaatgc gggcttcggt ggtacggatg ggcagtgcag atacttcagc
     2101 cgccggggta tcgataaagg tcagaacagc aatatcataa aatgaatcac ctccaagaat
     2161 ggctacctca tattttgaac gattgattga atagacataa atactgcctt tatcggttga
     2221 agcatcttca accacgtgag cgtttgtaat gacatatttt tttccacctt tttcgataat
     2281 aaaacctgat cccgacgcaa ctgagttact caggtcgaga gcctggtcat aagcgatatc
     2341 ggaattaccc cggaagccaa gaatctgcct ggtggactca accttgtaaa cagccaccgt
     2401 aactaccgat gataacaccg tttccagcat atccgaggta ttctgtgcgt agcctgaaca
     2461 accgaatact gtaatcagaa agaatatgac aaactttttc atacaacttt gtttagcata
     2521 gtatcagatg tttttcgcat aaaaaaacct gaccaatgcg ttcatttcat tgtgaaacat
     2581 aaaaacacac ctgtttcctt tggtgcatca tgttgcaggc tcactatgcc ctcaacgcag
     2641 caacttattg cagattgaag ctgtgagtgc cgtcgcaatc gccttcagaa acccagctat
     2701 aagttcctcc gctggtttcg caatatacat ctttccaacc ggaaacaaca tctatatcgc
     2761 ttttggccca gggtgccaat tgtccctgat aatagccgtc aacccaaata tggatataat
     2821 aaccggtcca attttgaaat tgaatagtgc accaggagcc tttcactttt ttgattttgg
     2881 atttcaccac atcaggcatt tcgcacggga ctttgtcttt aaggtcggaa ttagcgccac
     2941 gggtaccttc taccttttgc ttaacaaggt ctttgaattt ctgagctgag gcgctgttag
     3001 ccgttgcaaa aacaaatgca atcatcagta cactcaataa aacagttctt ttcatagctt
     3061 tcattttaaa aatttttaaa aagtatttta ttatatttat atgattcacg aacattggaa
     3121 gaaaccggca atccttttcc gaaaacattt tcaaatatag tacttttcat ttacaatgtt
     3181 accaattaaa ataattttaa gatttatttt cattacgtac gaatgaaaaa gaaaatgtat
     3241 tcatcaaact ttttcaccat aacttccagt caaactctaa actttcttta cggctcgtcg
     3301 gcctttgtgc tccattggtc aggcgttcca cttctttgct tacatagtct tttagttctg
     3361 aaactgaaac tattttatcg ccgctggcat cggcagcaag gtttttcagt ccattgagta
     3421 tgcaatatgt gaacacccca ttgttccatt ccggcgactc cagagcatat cccgtgcctg
     3481 ctgcagcaga aattacgaca gcgcctgagc ctgccgacaa attggcaaaa agctcctgca
     3541 tcagctcaaa actgttttgc aggccaaggc cggaaccttc tt
//
LOCUS       DAOSFP010000241         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24159_length_3577_cov_3.314026, whole genome shotgun sequence.
ACCESSION   DAOSFP010000241 DAOSFP010000000
VERSION     DAOSFP010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24159_length_3577_cov_3.314026"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PLI16_07490"
     CDS             complement(<1..185)
                     /locus_tag="PLI16_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HOH84440.1"
                     /translation="MKKVLFITYYWPPAGGAPINRILKFYQYLPEFGWEPVILTTEGG
                     DFPFEDDSLLKEVRPET"
     gene            complement(261..623)
                     /locus_tag="PLI16_07495"
     CDS             complement(261..623)
                     /locus_tag="PLI16_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH84441.1"
                     /translation="MADFDLKLRTRKFAVQVFKFAEKIPRSIGNDIVSKQIIRSSSSI
                     AANYRSVNRAKSQADFINKLVIVQEETDETLFWLEFISDLEILKNNTELTSLSNECEQ
                     ILSIITKSIITSKNKLKK"
     gene            complement(763..1944)
                     /locus_tag="PLI16_07500"
     CDS             complement(763..1944)
                     /locus_tag="PLI16_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84442.1"
                     /translation="MSQETGKITKSLDKVLLYAELNDYKGYNKYDALDSKILWFFSFG
                     NKFLRLLYSQVVMRAPINIRPLFFVPKTRNPKGIALFSMAYMNHYKAHGDANDLSQAE
                     TLLEWLLQNPSKTEWGMCWGYQHPWQDVGFFAPAHLPNRVVTYFVTTAMMDAYEITKN
                     QKYLEAAKSALTFLLKAPKVLYEDDTMKCLSYVPSEKINWIVMDVSILTGSIVARVNK
                     YVKDEHLASEARKLVNFVVDKQTDYGAWYYTHPAGDHPRKHDNYHTGYIVDAILDYMD
                     HSGDQSFMNQYLKGVAYYKENLFLPDGAPKWANDKTYPFDVHGSAQGIISFVKASRFD
                     KSYLDLAEKIANWAIDNMQNQKHGFFYYQKTSLFRKPYTLMRWSCGWMSRGLSELVNY
                     K"
     gene            2233..>3577
                     /locus_tag="PLI16_07505"
     CDS             2233..>3577
                     /locus_tag="PLI16_07505"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HOH84443.1"
                     /translation="MKTQKFSNQSRLTSVLGMMLFLIIPLSMISQKSYQITENRAFVN
                     RESLLTHQMATSDDKTLSPYFFIKSESGETEQLPLKSTGADVNIAGVIADVTVKQVYV
                     NTGKNVIEAIYIFPASTRAAVYSMKMKVGDRLITAKIQEKQKARQDYQQAINEGKSAS
                     LLEQQRPNVFQMNVGNILPGDTISIEMCYTELLIPEQGVYEFVYPTVVGPRYSNQPDA
                     LASANDKWVSNPYTHAGEKPTYAFNIGVNLATGMPIKDVRCPSHEMTINYENPASANL
                     RLKNTGVFEGNRDVVIQYRLADNKIESGLLLYQGENGGENFFLAMVQPPPKPSVDQIP
                     PREYIFIMDVSGSMYGYPIETSKKVLKNLIKNIRTTDKFNVLLFAGCANMLMPQSVYA
                     TEDNLQKAIKMIDQQQGSGGTELLAALKKALSVEKQSGFSRTFVIATDGYVDVEKE"
BASE COUNT         1114 a          752 c          677 g         1034 t
ORIGIN      
        1 tttgtttcgg gccttacttc tttaagcaga ctgtcatcct caaaaggaaa atcgccgccc
       61 tccgttgtaa gaataacggg ctcccagcca aattccggaa ggtactgata aaacttcaat
      121 atcctgttga taggagctcc acccgcaggg ggccagtagt aagttatgaa gaggactttt
      181 ttcataatag ttcagatttc agaattatga agtcagaatt atgaaatcag atttctgaat
      241 tcagaatttc aacatcttaa ttacttttta agtttatttt ttgaggtaat tattgatttt
      301 gtaataatag ataaaatttg ttcacattca ttcgagagag aagttaactc agtattattt
      361 ttcagtattt ccaaatcaga aataaattcc aaccagaaca aggtttcatc ggtttcttcc
      421 tgtacaatta ccagcttatt gataaaatct gcctgagatt ttgcacggtt cacggatcta
      481 taattagcag caatcgaact tgatgatcgg ataatttgct tggaaacaat atcatttcca
      541 atggatctgg gtattttttc ggcaaacttg aaaacctgaa ccgcaaattt tcttgttctt
      601 aattttaaat caaaatcagc catgaaagat aaaattaata tttaattcca aatttattct
      661 gacttctaac ttctgaattc tgcattctgt attctcatct ctgaattctg cattctaaat
      721 ttttcttcct caattctgac ttctgacttc tgacttctga atttatttat aattaaccaa
      781 ctcactcagt ccccggctca tccatccgca agaccaacgc atcagggtgt agggcttacg
      841 gaacaatgaa gttttctgat aataaaaaaa tccgtgcttc tggttttgca tattatcaat
      901 agcccagttg gcaatctttt cggcaaggtc aagataactt ttatcaaacc ggcttgcttt
      961 cacaaacgaa attataccct gggccgagcc atgcacatca aaaggataag ttttatcgtt
     1021 agcccatttt ggagcgccat caggtaaaaa caagttttct ttataatacg ccactccttt
     1081 caggtattga ttcataaatg actggtcgcc gctatggtcc atataatcaa ggatagcgtc
     1141 aacaatgtaa cccgtatggt aattatcatg ctttcgcgga tgatcaccgg cgggatgcgt
     1201 atagtaccat gcaccgtagt cggtttgttt gtcaaccaca aagtttacaa gtttgcgggc
     1261 ttcacttgcc aaatgttcat cctttacata tttattgacc cgtgccacta tggaacctgt
     1321 aagaatgctt acatccatga ctatccagtt gattttttcg gacggcacat aactgaggca
     1381 cttcatggta tcgtcttcat acaatacttt tggcgctttc agcagaaaag tcagcgcact
     1441 ttttgctgcc tctaagtact tttggttttt ggtgatttca taagcatcca tcatcgctgt
     1501 tgtaacaaaa tatgttacca cccggttagg caaatgcgca ggagcaaaaa aacctacatc
     1561 ctgccaggga tgctgataac cccagcacat cccccactca gtttttgaag ggttttgaag
     1621 cagccattca agcagcgttt ctgcctgaga aagatcattt gcatcaccat gtgctttata
     1681 atggttcata tatgccattg agaacaatgc aatccctttt ggatttcgtg ttttaggaac
     1741 aaaaaacaag ggcctgatgt ttataggagc gcgcattacg acctggctgt ataacaatcg
     1801 caaaaactta ttcccaaaag aaaaaaacca taagatttta ctgtctaaag catcgtattt
     1861 attatatcct ttataatcgt taagctcagc atacaagagt actttatcaa gcgattttgt
     1921 aatttttccg gtttcctgcg acatatacta aaatttgaag caaaattaac aatattgtat
     1981 ttacctgtgt aagatttcag cattctccct aacgcaataa ttgctccggc aaagaaacct
     2041 taaggatgta catggggatt taattaccca aatggggcat attaactgtc ccaaacatgc
     2101 ataaccctga tttactgcat attataattt tatttatttt tttatggaaa aatctgcaaa
     2161 cctcatctat aaaaatacaa attcaaaaaa gggcttttaa aaaaaattaa ataactcgta
     2221 aaaattaata ccatgaaaac acaaaaattc tcaaatcaaa gccggttaac cagcgtactg
     2281 gggatgatgt tatttctcat cataccctta agcatgattt cgcaaaaaag ttaccagatt
     2341 acagaaaaca gggcgtttgt taatagagag tcattgttaa cccaccaaat ggctaccagt
     2401 gatgacaaaa cgctgtcgcc ctattttttt atcaaaagcg aaagcggcga aacagaacaa
     2461 ctgcctctga aaagcaccgg cgcggatgtc aacatcgccg gagtaatagc tgatgttaca
     2521 gtaaaacagg tgtatgtgaa tacggggaaa aatgttattg aagcaattta catttttccg
     2581 gcatccacgc gtgcagcggt ttacagtatg aaaatgaaag tcggcgaccg cctgataacg
     2641 gcaaaaattc aggagaagca aaaagcccgg caggattacc agcaggcgat aaatgaaggc
     2701 aaaagtgctt cgctgctgga acagcaacgt ccgaatgttt ttcagatgaa tgttggcaat
     2761 attctgcccg gcgacaccat cagcattgaa atgtgttata ccgagctatt gattcctgaa
     2821 cagggggtgt atgagtttgt ttaccccact gtggttggcc caagatactc taaccagcca
     2881 gatgcccttg catccgccaa cgacaaatgg gtttcgaatc catacaccca tgcaggagaa
     2941 aagccaacct atgcctttaa cataggggtg aaccttgcta ccggtatgcc cataaaagat
     3001 gtgcgttgcc cttcgcatga gatgacaata aattatgaaa atccagccag tgccaacctt
     3061 cggctaaaaa acaccggagt ctttgaaggc aaccgcgacg tggttattca ataccgcctg
     3121 gcggacaata aaatagaatc gggattattg ttatatcagg gggaaaacgg tggggaaaac
     3181 tttttcctgg ccatggtaca acctcctcct aaaccctctg tggatcagat accgccgcgt
     3241 gaatatattt tcatcatgga cgtttccggc tccatgtatg ggtacccgat tgaaacctcg
     3301 aagaaagtgc tgaaaaatct aataaaaaac attcgtacca cagacaaatt caacgtactg
     3361 ctgttcgctg gctgcgccaa tatgcttatg ccgcaatcag tgtacgccac agaagacaac
     3421 cttcagaaag caatcaaaat gattgatcag cagcaaggat ccgggggcac agaattgctg
     3481 gcagccttga aaaaagccct gtcggtagaa aaacaatcag gcttttcacg aacctttgta
     3541 attgctaccg atggctatgt ggatgtagaa aaggaat
//
LOCUS       DAOSFP010000242         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24252_length_3568_cov_4.338457, whole genome shotgun sequence.
ACCESSION   DAOSFP010000242 DAOSFP010000000
VERSION     DAOSFP010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24252_length_3568_cov_4.338457"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1435)
                     /locus_tag="PLI16_07510"
     CDS             complement(254..1435)
                     /locus_tag="PLI16_07510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOH84444.1"
                     /translation="MQKRIFVTGMGIITALGKNLPETFDALVARRSGIGSIKHLNTIH
                     RDKIPVAEVPQTNEELAGLCGLQGRTDLTRTTLLAMVAVNEAIQTAQINDFGETGLIS
                     ANTVGGMDKSEQYYYDYLKLKKVNPFISTSDCADSTEKIADHLGISKFVTTISTACSS
                     ASNAVLLGARMIQNGTLKRVIVGGTECLTKFHLNGFNALKILDYEPCKPFDKNRAGIN
                     LGEGAAYLVLESEDVAAGTGFELKGWGNACEAFHQTASSPEGDGAYLAMNKALEMSGL
                     QPGDIDYINAHGTGTDNNDLSEGRAIQRLFAPKLPLVSSTKPYTGHTTSAAGTVESII
                     TLLGMKNNMIFPNLNFNQQMPELTFSPVCCLMKNIEQKHVMTNSFGFGGNNTTLIFSK
                     I"
     gene            complement(1442..1705)
                     /locus_tag="PLI16_07515"
     CDS             complement(1442..1705)
                     /locus_tag="PLI16_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOH84445.1"
                     /translation="MEEQELIEKLRKEIIQQLNLEDLNPEDIDPDAALFGEGLGLDSI
                     DALEIIVLLEKNYGIKIEDPKEGKKIFASIRTLAKYIIDHQKA"
     gene            complement(1712..2350)
                     /locus_tag="PLI16_07520"
     CDS             complement(1712..2350)
                     /locus_tag="PLI16_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84446.1"
                     /translation="MHITKTTYISRNTIWQDNRIIHQQDETVSYQDFVKAAFKLLCAP
                     YPKFYKMDSMSKLGYLATDILLKDTDLLSRYAKEEIGLVFLNSSSTILTDTEHQQSIN
                     DRSNYFPSPAVFVYTLPNIMLGEICIKNGFFGENALLISEKFDAATLHTQVKLLAEAG
                     QIQACIAGWTEINEHHFEAFSMLIEPDDKAENSKEKCTFDVENIQKLYTRKY"
     gene            complement(2356..3513)
                     /locus_tag="PLI16_07525"
     CDS             complement(2356..3513)
                     /locus_tag="PLI16_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOH84447.1"
                     /translation="MHQVFVCGGNVITSLGFSTEEHIDKIKKEISGIRLIQDNSLYPE
                     DFYASLVDNQRLENEFASLSGPGQFTRFEKLMILSARKALAQNQHIDPASEKTVFVIS
                     STKGNINLLEENQKAAFEPERIFLWKSAEIIARYFGNNNKPLIISNACISGVLAITAA
                     SHLLRSGLYENAVVIGGDIASEFVISGFLSFKSLANGICKPFDKDRSGLNLGEGAGTL
                     ILSTKPEQKNGLPLIKVTGGASNNDANHISGPSRTGEGLFLAITRAMKMAQIHPEQID
                     FISAHGTATPYNDEMESKAFSSAGLSTVPLNSLKAYFGHTLGAAGIIESLISIYGLTH
                     DIIFKTLGFNELGVPEKITVVDTLTAKVQKNILKTASGFGGSNAAVVFAKD"
BASE COUNT          874 a          727 c          833 g         1134 t
ORIGIN      
        1 tatttgacta ttaactattt gactaatgac tatttgacta atgactattt gactattaac
       61 tatttgacta atgactattt gactaatgac tatttgacta caaaatacaa gttacaagtt
      121 gcacaaaaca aagaactatc ttcccacttt ttactttcaa ctttttactt tcaacttgct
      181 actttcaact tgctactttc aacttgctac tttcaacttg ctactttcaa ctaataacta
      241 tttgactatt taactatatt tttgagaata tgagcgttgt attatttccg ccgaaaccaa
      301 acgaattggt cataacatgc ttttgctcaa tatttttcat caaacagcat acagggctaa
      361 atgtgagctc gggcatctgt tgattgaaat tcagatttgg aaaaatcata ttgtttttca
      421 tgcccagcaa ggtgatgata gactctacgg taccggcagc gctggtggtg tgcccggtat
      481 agggcttcgt ggaacttacc aggggcagtt taggggcaaa gagccgttgg atggcgcggc
      541 cttctgaaag atcgttattg tcagttccgg tgccatgtgc attgatataa tcaatatcgc
      601 caggttgtaa accgctcatt tccaacgctt tattcatggc taagtaagct ccgtcacctt
      661 ccggagatga cgcggtttgg tggaaagcct cacaggcatt tccccatcct tttaattcaa
      721 aaccggtacc ggcggccaca tcttcagatt ccagtaccag gtaggctgct ccctctccaa
      781 ggtttatgcc ggcgcggttt ttgtcgaagg gcttacaagg ttcataatcc agaattttca
      841 gggcattgaa accgttgaga tgaaacttgg tgaggcattc ggtgccgcca acgattaccc
      901 gtttcagtgt gccattctgt atcatcctgg cgccaagcaa taccgcattg gatgccgagg
      961 aacaggcggt gctgatcgtg gtgacaaatt tactgatacc tagatggtca gctattttct
     1021 cggtgctgtc ggcgcaatca cttgttgaaa taaagggatt gacttttttt aatttgaggt
     1081 agtcgtagta gtattgttca cttttatcca tgccgcctac ggtgttggcc gaaataaggc
     1141 cggtttcgcc gaagtcgttt atttgtgctg tttggatggc ttcattgacg gcaaccatgg
     1201 cgagcagggt agtgcgggtg aggtcggtgc ggccttgcaa accacacaaa ccagccagtt
     1261 cctcgttagt ttgaggtacc tcggccaccg gaatcttgtc ccggtgtatc gtgttgaggt
     1321 gtttgatgct gccaataccc gaccggcggg caacaagagc atcaaaggtt tccggcaggt
     1381 tttttcccag ggcggtgatg atacccatac cggtaacaaa gatcctcttc tgcatcaatt
     1441 attaagcttt ttgatggtca ataatgtatt tggccagtgt ccggatagat gcaaatattt
     1501 ttttgccttc tttagggtct tcgattttga taccgtaatt tttttcgagc aatacaataa
     1561 tttccagggc gtcgatagaa tcaaggccta atccttcgcc gaaaagagca gcatcaggat
     1621 cgatatcttc ggggttgagg tcttcaaggt tgagttgctg gataatttct tttctgagtt
     1681 tttcaatcaa ttcttgttct tccatagtgt attaatattt tctggtatat aatttttgaa
     1741 tattttcgac gtcaaaagta catttttctt ttgaattttc tgccttatca tcaggttcga
     1801 tgagcatgga aaaggcctca aagtgatgtt cgttaatctc tgtccatccg gcaatacagg
     1861 cttgtatctg accggcttca gccaggagtt ttacctgggt atgcagtgtt gctgcatcaa
     1921 atttttcgct gataagcagt gcgttttccc cgaaaaaacc gttttttatg catatttcgc
     1981 ccagcatgat gttgggcagg gtataaacaa acaccgcagg gctggggaaa taattactcc
     2041 ggtcgtttat tgattgttga tgttccgtgt cggtgaggat ggtggacgaa ctgtttaaaa
     2101 ataccagtcc aatttcttcc ttggcgtagc gggatagtaa gtccgtatct ttcagcaaga
     2161 tatctgttgc cagataaccc aacttactca tactgtccat tttataaaac ttgggatagg
     2221 gtgcacacaa gagtttaaag gcggctttga caaaatcctg gtaactgacc gtttcgtctt
     2281 gttgatggat tatccggttg tcttgccaga ttgtattgcg tgagatatat gtggtttttg
     2341 ttatatgcat gtacattaat cttttgcaaa cacgacggca gcgttgctcc ctccaaaacc
     2401 ggaggcggtt tttaatatgt ttttttggac cttagcggtc agcgtatcaa cgacagtgat
     2461 tttttccggc acgcctaatt cgttaaatcc caatgtttta aatatgatat cgtgtgtcaa
     2521 tccataaatt gaaatcaatg attcgatgat acctgcggct cccagggtgt gtccgaagta
     2581 ggctttcagg ctgttgagtg gtaccgtgct caggccggcg ctgctgaatg cttttgattc
     2641 catttcatcg ttatacggtg tggcggtgcc atgagccgaa ataaaatcta tttgttctgg
     2701 gtggatttgc gccatcttca tggcccgtgt tattgccagg aacagtcctt cgccggtgcg
     2761 agaaggccct gagatatggt tggcgtcgtt gttgcttgct cctcctgtga ctttaatcag
     2821 gggaagtccg tttttttgtt cgggttttgt gctgaggatc agcgtgccgg caccttcgcc
     2881 aaggttcagt ccgctgcggt ctttatcgaa gggtttgcaa attccgttgg ccagagattt
     2941 gaacgataaa aatccggaga ttacaaattc agaggctatg tcgccgccta tgaccacagc
     3001 gttttcgtaa aggcctgacc gtaagaggtg actggcggcc gtgatagcta agacacctga
     3061 aatacaggcg ttagatatta tcaggggctt gttgttattg ccaaaatacc gggcaattat
     3121 ttctgctgat ttccaaagaa atatcctttc gggttcaaaa gccgcttttt ggttttcttc
     3181 gaggaggttg atgttgcctt ttgtagaaga aatcacaaaa acggtttttt ccgaagcagg
     3241 gtcgatgtgt tggttttgcg ccagggcttt ccgggcagag agtatcatta atttttcgaa
     3301 ccgggtaaat tgcccggggc ccgaaagaga ggcaaattca ttttcgagac gttgattatc
     3361 aaccagtgag gcataaaaat cttcaggata cagggagtta tcttgtatca ggcggattcc
     3421 tgagatttcc ttttttattt tgtcgatatg ttcttccgtg gagaatccga gagaagtaat
     3481 tacgttaccg ccgcatacaa aaacctgatg catgataaaa aattaagtgg caaaattatg
     3541 aaaaaaatct tttggtttta ctttcagg
//
LOCUS       DAOSFP010000243         3562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24311_length_3562_cov_4.498717, whole genome shotgun sequence.
ACCESSION   DAOSFP010000243 DAOSFP010000000
VERSION     DAOSFP010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24311_length_3562_cov_4.498717"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="PLI16_07530"
     CDS             <1..392
                     /locus_tag="PLI16_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84448.1"
                     /translation="KIDDLKIELGYALLPLVILVCMHKRYVHTWLIAVVYFLVSSLSV
                     FFNLMNTSAMLSRALEQMPEIFQVPQGISEDEFTTMLKIAFGFSIVLSALFELAIMII
                     FYVKRKYFRDYRPEEQLTAENSSGQNQ"
     gene            complement(439..1164)
                     /gene="lptB"
                     /locus_tag="PLI16_07535"
     CDS             complement(439..1164)
                     /gene="lptB"
                     /locus_tag="PLI16_07535"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOH84449.1"
                     /translation="MILRTDNLIKKYRSRTVVKNVSLEVKQGEIVGLLGPNGAGKTTS
                     FYMIVGLIKPNEGRVFLEDIDITDEPMYKRAQKGIGYLPQEASVFRGLSVEDNIMAVL
                     EFTKLTKSQQKQRLEELLDEFGLQHVRKGKGITLSGGERRRTEIARALAVSPHFILLD
                     EPFAGVDPIAVEDIQTIVAKLKTKNIGVLITDHNVHETLSITDRAYLLFEGSILKAGT
                     AEDLANDPQVRKVYLGENFELRK"
     gene            complement(1196..>3562)
                     /locus_tag="PLI16_07540"
     CDS             complement(1196..>3562)
                     /locus_tag="PLI16_07540"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOH84450.1"
                     /translation="KDPMPFVNVYFAGTGVQTTTDFDGKFSIETKLAKDSLTAQYMGY
                     NTAKKAVVKNKFQYIDFELNVLSFQLTEAVILPGENPAEVLLKKVIYNKPVNNREFFD
                     YFQYKAYTKVQIDANNYSENLQNNLLLRPFKFVFENSDTSTVSGKVYLPLFFSESMSD
                     IYYRKEPKKYREIIGASKVSGMENKTISQFLGDTYQKINIYDNYNELFEKNFVSPIAN
                     FGLSYYKYYLVDSGFIGNRWCYHIMFKPRRSQELTYTGNLWVHDTTYGIVKINMRVVK
                     DANINIINEILFDQEFALVDNTFWMPVKDYMVADLNITENTSKIIGIYLHKTTAYHDF
                     IINQPKENKFYNSPEAIFIADSAVEKKDDDWTKIRPEELSKEEQQIYKLIDTVQTLSA
                     YKLYKGIGEAILGNYIRMGYIDVGPLLQMISINPVEGQRFRLGFRTSSQLSKKFQLLM
                     HGAYGTYDDAFKYGISAFYMFSKNPHTIVGFRYKYDIEQLGQSTNAFSEDYFLRGILS
                     RNKSDKLTMAKDLLIFAEKDWFMGFYNDLFLKYRVLYPLPGEEAFKFKDGNGTTTKDV
                     ITIAEIQLLTHFAYNEKFVSGAFRRRSIGTKYPIVDVTYSYGIKNCFRSDYEYHKLDL
                     RIRQWFNIGTAGWSRYMIGAGKVWGTLPFQLLKIHEGNETYLWDEYAFNTMNYYEFVS
                     DAYFSWYYTHHFDGFFLNRIPLIKKLRWREVVHFRGLVGSITSKNKNYNFFPESTYFL
                     NKGPYCEAGVGIENIFKFLRIDGIWRLNYLDHPHTNKFIVMASLRFNF"
BASE COUNT          911 a          737 c          809 g         1105 t
ORIGIN      
        1 tcaagatcga cgatctcaag atcgagctcg gctacgcgct gctgccgctg gtcatcctgg
       61 tgtgcatgca caagcggtac gtacacacct ggctcatcgc tgtggtgtat tttttggtga
      121 gttcgctgag cgtgtttttt aacctgatga atacctcggc tatgttgagc agggcgttgg
      181 aacaaatgcc cgaaatattt caggtgccac aaggtatcag cgaagacgaa ttcaccacta
      241 tgctgaagat tgcctttggc ttttccattg tgctttctgc gctgtttgaa ctggccatta
      301 tgataatttt ctatgtgaaa cgcaaatatt tcagggatta tcgcccggaa gaacagttga
      361 cagcggaaaa cagttccgga caaaaccagt aagcgatttt tttgttgtgt tgcaggaagt
      421 tttttctgaa ggagactttt attttcgtaa ttcaaagttt tcgccgaggt acactttgcg
      481 tacctgtggg tcgttggcaa ggtcttcggc ggtgccggct tttaatatcg atccttcgaa
      541 cagcaggtaa gccctgtcgg tgatagaaag ggtttcgtgc acattgtgat cggtgatgag
      601 gacaccgata ttttttgttt tgagcttggc tacgatagtc tgaatgtctt caacggcaat
      661 ggggtcaacg cccgcaaaag gttcgtctaa caggataaaa tgagggctta cggccagcgc
      721 ccttgcaatc tcggttctgc ggcgttctcc gcccgagagg gtgatgcctt tacctttgcg
      781 tacatgctgt aacccgaatt catcaagtaa ttcttcgagg cgttgttttt gctggctttt
      841 ggtgagtttg gtaaattcaa gtacggccat aatgttgtct tctacactca agcccctgaa
      901 aacagaagct tcctgcggaa gatagccgat gcctttttgg gcacgcttat acatcggctc
      961 gtcggtaata tcaatgtctt caagaaaaac ccggccttcg ttaggtttta tcaatccgac
     1021 aatcatataa aaagacgtgg tttttcctgc cccgttaggc ccgagcaatc ctactatctc
     1081 gccttgtttt acttccagcg aaacattttt caccacagtg cgtgaacggt atttttttat
     1141 gagattatcc gtgcgtagga tcatgtatta caaaaagaat tgttgcaaaa ttacattaaa
     1201 aattaaaccg taacgatgcc atcactataa atttattagt atgaggatgg tccaggtagt
     1261 ttaatcgcca gatgccgtcg atacgcaaaa atttgaaaat attttcgata cctacgccgg
     1321 cttcacaata aggtcctttg tttaaaaaat aggtgctttc aggaaaaaaa ttatagtttt
     1381 tattcttgct ggtgatgctt cccaccaggc ccctgaaatg tactacctca cgccagcgca
     1441 atttttttat cagggggatg cggttcagaa aaaacccgtc gaagtggtgc gtgtaatacc
     1501 aactgaaata cgcatcgctg acaaattcat aatagttcat cgtgttaaac gcatattcat
     1561 cccacaggta agtttcgtta ccttcatgaa ttttcagcaa ctgaaagggt agtgtccccc
     1621 acaccttgcc ggcgccaatc atgtaccgcg accagccggc cgtaccgata ttgaaccact
     1681 gccggatgcg aaggtccagt ttgtgatatt catagtcgct tcgaaaacag ttttttattc
     1741 cgtaggaata tgtaacatcc acaataggat attttgtgcc tatgcttctg cggcggaacg
     1801 caccggaaac gaatttttca ttataggcaa agtgtgtcag cagttgtatt tcagcgatag
     1861 taatcacgtc tttggtggtg gttccgttgc catctttaaa tttaaaagct tcttctccgg
     1921 gcaaaggata aagcacacgg tattttaaaa acaggtcgtt ataaaaaccc atgaaccagt
     1981 ctttctcggc aaagatcagc aagtctttgg ccatggtgag cttgtcggat ttgttacgcg
     2041 aaagaatacc tctcagaaag taatcttcac tgaaagcatt ggtgctttgc cctagctgtt
     2101 caatatcgta tttgtatcgg aaaccaacta tggtgtgagg attttttgaa aacatataaa
     2161 aagcgctgat accatatttg aaggcgtcgt cgtaagtgcc ataagcgcca tgcatcagca
     2221 actgaaattt tttgcttaat tggctgctcg tgcgaaaacc cagcctgaac cgctggcctt
     2281 caacggggtt gatgctgatc atctgcaaca atggccctac atcaatgtaa cccatgcgga
     2341 tgtaattacc caaaatagct tctccgatac ctttgtaaag tttatatgca gaaagcgttt
     2401 gcaccgtatc gatgagttta taaatctgct gttcctcttt gctaagttcc tccggcctta
     2461 tttttgtcca atcatcatct tttttttcta cagcgctgtc ggcgataaat atggcttcgg
     2521 gactattgta gaatttgttt tcttttggct ggttgatgat aaagtcgtgg taagcggttg
     2581 tcttgtgaag gtatatcccg atgattttac tggtgttttc agtaatgttg agatctgcta
     2641 ccatataatc ttttaccggc atccagaagg tgttgtccac cagggcgaat tcctgatcga
     2701 acagtatttc gttgatgata ttgatgttgg catccttaac gacacgcatg ttgattttta
     2761 ctattccgta ggtggtgtcg tgtacccaca ggttgccggt gtaggtgagt tcctgcgaac
     2821 ggcgcggttt gaacatgata tgataacacc accggttgcc gataaacccg ctgtcaacca
     2881 gataatattt atagtatgat aaaccaaaat tggctatcgg gctgacgaag tttttttcaa
     2941 acagttcgtt atagttgtcg taaatattga tcttttggta ggtgtcaccc aaaaattgtg
     3001 aaatggtttt gttttccatg cccgacactt tgcttgcgcc gatgatctcg cggtattttt
     3061 tgggttcttt tctataataa atgtccgaca tggattcgga gaaaaacaag gggagataca
     3121 cttttccgct aaccgtggat gtgtctgagt tttcaaaaac aaatttgaac ggccgcagca
     3181 ggaggttgtt ctgcaggttt tcggaatagt tgttggcgtc aatctgcact ttggtgtagg
     3241 ccttatactg gaaataatca aagaattcac ggttgttgac aggcttgttg tagatgactt
     3301 tttttagcag cacttcggca ggattttcgc cgggtaagat aactgcttcg gtcaactgaa
     3361 atgataagac atttagctca aaatctatgt attgaaattt gttttttacc acggcttttt
     3421 ttgcggtatt gtaacccata tattgcgcag tgagcgagtc tttggccaac ttggtttcga
     3481 tagaaaattt gccgtcaaaa tcggtggtag tttgcacacc ggttcccgca aaatagacat
     3541 ttacaaaagg catggggtcc tt
//
LOCUS       DAOSFP010000244         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24458_length_3545_cov_3.147278, whole genome shotgun sequence.
ACCESSION   DAOSFP010000244 DAOSFP010000000
VERSION     DAOSFP010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24458_length_3545_cov_3.147278"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..2307)
                     /locus_tag="PLI16_07545"
     CDS             complement(199..2307)
                     /locus_tag="PLI16_07545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOH84451.1"
                     /translation="MKKIIIMLMACATVSFFACNKGKKNSDNPFFSDYTTPFKVPPFD
                     KIDTTHFMPAFIEGMKQQNDEITKIVNNTEAPTFDNTILPFDKSGLLLTKVSNVFFNL
                     TESTTNEQIQAIERKIMPMYSKHSDEIFMNAKLFERIKTVYENRNEMKLDDQQIRVVE
                     KYFRDFERLGANLPKDKQEELKKINEELSLLSTKFGENLLAENKNFKMVVDKKEDLEG
                     LPQGSVDAAAEAAKAQNLEGKWVFTIDKPSMIPFLTYAKNRDLREKLYRGYFMKGDNA
                     NTNDNNEVIAKIAKLRVQKAKLLGFDSYAAYVIDENMAKTPAKVNDFLAQLWEPALKM
                     AKNEVVELQKIADKEGGKFKIQPWDWWYYSEILRKEKYDLDENEMKPYFSLENTINGI
                     FYVANKLYGITFEKLTNLPVYFPEVETYQVKEADGKHLGLIYFDFHPRDSKRGGAWCG
                     GFESAGWRDGKKVDPIVSIVCNFTKPTKETPALLTYDEVETLFHEFGHGLHSLFTEGK
                     YSRTAGSVPRDYVELPSQFMENYASEPEVLKYYAKHYKTGEVIPDKLIEKIQNSSKFN
                     QGFITTELLAAAILDMDYHNLTEPKDLNIKEFEKAAMDKIGLIPEILPRYRSTYFSHI
                     WGGGYAAGYYVYLWAEVLDADAFDAFKQSGDIFNKDLATKFRKYCLQESGDGEGMDQY
                     RKFRGQDPSIDPLLKRRGFK"
     gene            2450..3280
                     /locus_tag="PLI16_07550"
     CDS             2450..3280
                     /locus_tag="PLI16_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOH84452.1"
                     /translation="MAASKNKAWYLYAGIGVIALIFLIFFYGPEKNDEKGFKDNDSSG
                     NNKNIEKKVFTELAPGAGPAGQIIHNKYYTLSYNKQYKQANWVCYKLTSQMLKNKTGR
                     TDDFRPDAEAGAGAATPEDYKNSGYHRGHLCPAGDMKFSAKAMSETFVMTNISPQDAD
                     LDGGIWRELEEQVREWAREHKELYITTGPVFGKHVKTIGRNKVAVPEYFFKVILDYKQ
                     PGVKGIGFIFKNEACADQLPFLAVSIDSVEAFTGIDFFSALPDADEQKIEKTARWSMW
                     "
     gene            3356..3432
                     /locus_tag="PLI16_07555"
     tRNA            3356..3432
                     /locus_tag="PLI16_07555"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3390..3392,aa:Pro,seq:ggg)
BASE COUNT          951 a          726 c          832 g         1036 t
ORIGIN      
        1 aaagaaaaaa tggcaaggat agccgaagta ccgataaagc cagaggtgcc aggaaaaagt
       61 caaagaaaag gtaaaaacct gtatttcagc cattggccgg aagaccacac aggaaaattc
      121 agaattgttg atcagatagt tcaaaaacaa taaagcccgg aaaaccgggc tttattgttt
      181 ctttttgcgt actctatctt atttaaatcc ccttcgtttc agcaacgggt cgatagaagg
      241 atcctgtccg cggaatttgc ggtactggtc cataccttcg ccatcgccac tttcctgcag
      301 acaatatttg cggaattttg tggcaaggtc tttattaaat atgtcgcctg attgtttgaa
      361 agcgtcgaaa gcgtcggcat ccaacacttc agcccacaga tatacataat atccggcagc
      421 atagccaccg ccccagatgt gtgagaaata agtggacctg tagcggggta aaatttcggg
      481 gataagcccg attttatcca tggctgcttt ttcaaattct ttaatgttta agtctttagg
      541 ttcggtcagg ttgtgatagt ccatatccag gatggctgca gcgagaagtt cggtggtgat
      601 aaatccctgg ttgaatttgg aactgttctg aatcttttcg atgagcttgt cgggaataac
      661 ttcgccggtt ttgtaatgct ttgcatagta tttaagcact tcgggttcgc tggcatagtt
      721 ttccataaat tgtgaaggca gttccacata atccctgggc acgctgcctg cagtgcggga
      781 atattttcct tcggtgaaga gagagtgcag tccatggcca aattcgtgga ataaagtctc
      841 cacttcatcg taggtaagca atgcaggagt ctccttggta ggttttgtaa aattgcagac
      901 gatagaaact atgggatcta cttttttgcc gtcgcgccag cctgccgatt caaagccgcc
      961 gcaccaggca ccgccgcgtt ttgaatcgcg cggatgaaaa tcaaaataga ttaagcccag
     1021 gtgtttgcca tcagcttctt ttacctgata ggtttccact tcgggaaaat ataccggcag
     1081 gttggtcaat ttttcaaaag tgatgccata gagtttgttg gccacataga aaataccgtt
     1141 gatagtattt tccaggctga aataaggttt catttcgttt tcgtcgaggt cgtatttttc
     1201 tttgcgtaat atttcagaat aataccacca atcccagggc tgaattttaa attttccacc
     1261 ttctttatca gcgattttct gcaattcaac cacttcattt ttagccattt tcaaggcagg
     1321 ctcccataat tgagccaaaa aatcatttac tttagcggga gtcttggcca tattctcgtc
     1381 tatcacataa gctgcataac tgtcgaatcc cagtaatttg gctttttgaa cgcggagttt
     1441 tgcaattttg gcaatcacct cattgttgtc gttggtattg gcgttatcgc ctttcataaa
     1501 atatcccctg tacagcttct cacgcaaatc acggtttttg gcgtatgtaa gaaaaggaat
     1561 catgctgggt ttgtcgatgg taaaaaccca ttttccttcg aggttctgag ctttggcagc
     1621 ttcggcagcg gcatccacag agccctgggg cagtccttcc aggtcttctt ttttgtccac
     1681 caccatttta aaatttttgt tttcggccag caggttttca ccaaatttgg ttgaaagcaa
     1741 ggacagttct tcgttgattt tcttgagttc ttcctgttta tcttttggca ggttagcacc
     1801 gaggcgttcg aaatcgcgga aatacttttc cacaacacgt atctgctggt cgtcgagttt
     1861 catctcgtta cgattttcat atacggtttt tatacgttcg aacaatttgg cattcatgaa
     1921 aatctcatcg ctgtgcttgg aatacatcgg catgatctta cgctcaatgg cctgaatctg
     1981 ctcattggtg gtagattcgg tgaggttgaa aaacacgttg gacactttag taagcaacag
     2041 tcctgatttg tcaaaaggca gtatggtatt gtcgaacgtg ggtgcttccg tattattgac
     2101 aattttggtg atttcgtcat tttgttgttt catgccttcg ataaaagcag gcataaagtg
     2161 ggtggtgtca atcttgtcga aaggcggcac cttaaacggg gtggtataat ccgaaaaaaa
     2221 cggattatcg ctgttttttt tgcctttgtt acaggcaaag aaactaacgg tggcacaggc
     2281 cattaacata atgatgattt ttttcatttt taaatttgtt tacaattatg gaccaaaagt
     2341 acaaaaaaag tttgcatatt tacagttgtt gcaaagagcg ggcatatttt ttttgacccg
     2401 ccggtcgaga accagggaaa attgtgattt ttgcatgaaa taaaaacgaa tggcagcaag
     2461 caaaaacaag gcatggtatt tatatgcagg tataggcgtg atagcgctta tatttctgat
     2521 ttttttttac gggcctgaga aaaacgacga aaagggtttt aaagataacg attcgtctgg
     2581 caacaacaaa aacattgaaa aaaaagtttt tacagagttg gcgcccggtg cgggaccagc
     2641 cgggcaaatc atccacaata aatactatac gctcagctat aacaagcaat acaaacaagc
     2701 caactgggta tgttacaagc tgacgtcaca aatgctgaaa aacaagaccg gccgtaccga
     2761 tgacttcagg ccggatgctg aggccggagc aggtgctgcc acaccggagg actataagaa
     2821 ctcaggctat cacagggggc acctctgccc tgccggcgac atgaaattct cggctaaggc
     2881 catgtcggag acctttgtga tgacgaacat ctcgccacag gatgcagatt tagacggtgg
     2941 tatctggcgg gagctggagg agcaggtccg ggaatgggca agagaacaca aagagttgta
     3001 tattaccacg gggccggttt ttggcaagca cgtaaaaact ataggcagaa ataaggtggc
     3061 tgtacccgag tattttttta aggtaatcct cgactataag cagcccggtg tgaaaggcat
     3121 aggatttatt tttaagaacg aggcctgtgc cgaccagctg ccatttcttg ctgtgagcat
     3181 cgattcggtg gaggccttca cgggcataga ttttttcagt gcgctgcccg atgccgatga
     3241 gcaaaagata gaaaaaacag caagatggtc tatgtggtga ttattagtct tttagcaatt
     3301 tttttcgtca attaattttt tttcaaaaaa agattctatc tttgcagcac attttcgggg
     3361 cgtggcgcag ttggctagcg tacttgcatg gggtgcaagg ggtcggaagt tcgagtcttc
     3421 tcgccccgac caataaaatc aagggtttca gagggtctga aatccttttt tgttttcggt
     3481 tttacacaca agaaaaacac ggaggcacgg atccgccgcg gcggatccgt gcctccgtgt
     3541 ttttc
//
LOCUS       DAOSFP010000245         3544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24465_length_3544_cov_3.797077, whole genome shotgun sequence.
ACCESSION   DAOSFP010000245 DAOSFP010000000
VERSION     DAOSFP010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24465_length_3544_cov_3.797077"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /gene="tyrS"
                     /locus_tag="PLI16_07560"
     CDS             <1..1096
                     /gene="tyrS"
                     /locus_tag="PLI16_07560"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOH84453.1"
                     /translation="PIALVGGATGMIGDPSGKSEERNLLDEETLRANQAGIQKQLEKF
                     LDFSEGPVQAEIVNNYDWMKDFNFLSFLRDVGKHITINYMMTKDSVQKRMQGGSGISF
                     TEFTYQLVQGYDFFWLNINKGCKLQMGGADQWGNITTGTELIRRKGGTEAFAITCPLI
                     TKADGTKFGKTEAGNVWLDPQKTSPYKFYQFWLNCSDEDSEKYIKIFTLLTREEVEEL
                     VRQHQEAPHLRVLQKALAKEVTMRVHSEKDYQAAVEASGILFGKGTEETLRSLDEETI
                     LSVFEGVPQSFISADDLEKKMDVIEFLTTQTGILSSKSEARRMFGEGCIFINKQKMSE
                     GAVIDKTFLLNNKYILVQKGKKSYYLVISR"
     gene            complement(1104..>3544)
                     /locus_tag="PLI16_07565"
     CDS             complement(1104..>3544)
                     /locus_tag="PLI16_07565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOH84454.1"
                     /translation="KKTEQALIQEEDKFRNVIEQATEGISINDIHGRIIEWNNALVEI
                     TGITKDQALGKYHWDIEYEMMTPKKKTKEFYEKIKQNALNAFQEDAFPEKFKKIEGKI
                     LRKDGEERYVDYSIFLIKTQNASYISRVVRDITGQKKFMQALQRSEAQYRALFEHSSV
                     AILIINPENYKIMAANQKAAETYGYTIDDFFEMPLEQITREIDLDKQQIENIKNHIVT
                     KDFNTIHLNRKGKEIHMLVDGSTVEYGGIPAVMNFNRNITEIKRLEKARETVYKISQL
                     THTVDNMYDMYKSIHKIINEILPARNFYIALYDDASDIVSFPYFVDEKDPSPSPHRLK
                     RGLTEYVLRHGKPVVTTSELLKQLELSGEIELIGAYSETWMGVPLITHNKVIGVVAVQ
                     SYSTAIQFTENEKNLLVYVSEQIAQQIYKKQAEEEILRGKAKAEESDRLKTSLITNMN
                     HDLRTPMTGILGFASLLKNRIKDNETLSMVDTIIESGNKLMSIINSILQLSQLEAGNK
                     QITLITSEVNKYVEMPLGQLEIKARQKNLSIVRQYEDDIVAVFNESYLIQSITNIVNN
                     AIAYTEKGSITIKTGLSEHQGKKFAHIKIEDTGIGIDANNFSKIFQEFRQVSEGLNRR
                     YDGSGLGLSISKKMIEMMGGHILVESQLGKGSVFTILLPLASEKNVKKETPVKEKEPH
                     KIARSRKPHNLPKVLVVEDNKINGELIFAYLKQDYEVDIAKTGFLAIELVKRSEYNIV
                     LMDINLGEGMNGMQAAREMHKINKDLPIVALTGYSTEKEITTILKHDFIGYILKPVDR
                     VSLYKVLEKTIAKK"
BASE COUNT          884 a          693 c          815 g         1152 t
ORIGIN      
        1 gcctatagca ctggtaggcg gtgccaccgg catgattggc gacccgtcgg gcaagtcgga
       61 agaacgtaat ttgctggatg aagaaaccct gcgcgctaat caggcgggca ttcaaaaaca
      121 gttggagaaa ttccttgatt tttcggaagg ccccgtacaa gcggagatcg tgaataatta
      181 cgactggatg aaagatttta attttttgag ttttttgcgc gatgtgggca aacatatcac
      241 cattaattat atgatgacaa aagactcggt gcaaaagcgg atgcagggcg gcagcggcat
      301 ttcgtttact gagtttacct atcagttggt gcaggggtat gattttttct ggcttaatat
      361 caacaaaggt tgcaagctac agatgggcgg tgccgatcag tggggtaaca ttactacagg
      421 aacagaatta atcaggcgca aaggcggaac agaagctttt gccattactt gtccgctgat
      481 taccaaggcc gatggcacta aattcggaaa aaccgaagcc ggcaatgttt ggcttgaccc
      541 gcaaaaaact tctccatata aattttatca gttttggctg aattgttctg atgaggattc
      601 tgaaaaatat atcaaaattt tcaccctgtt gaccagggaa gaggtggaag agcttgtgcg
      661 gcaacatcag gaggctccgc atttgcgggt tttgcagaag gcactggcaa aagaagtgac
      721 gatgagggtg cattcggaaa aggattacca ggcggcggtg gaggcttccg gcatattatt
      781 tggcaagggg actgaagaaa cccttaggtc gctcgacgaa gaaaccattt tgtcggtatt
      841 tgaaggcgtg ccacaaagtt ttatttccgc agatgatttg gagaaaaaga tggatgttat
      901 agaatttctg actacccaaa caggtatctt gtcatcaaaa agcgaagcaa gaaggatgtt
      961 tggcgaaggg tgtattttta tcaataagca aaaaatgtca gagggtgccg tgatagacaa
     1021 gacttttctc ttaaacaaca aatacatact cgtgcagaaa ggcaaaaagt cctattatct
     1081 ggttatcagc cgatgacaag gggttatttt ttcgcaattg ttttttcaag taccttgtaa
     1141 agcgataccc tgtccacagg tttgaggata tatccgataa agtcatgttt tagtatcgtg
     1201 gtaatttctt tttcggtaga atagccggta agcgccacga tgggcaggtc tttgtttatt
     1261 ttgtgcattt cgcgggcggc ctgcattccg ttcatgcctt caccgaggtt gatatccata
     1321 agcacaatat tgtattccga ccttttaacc agctcaatgg ccagaaaacc tgttttggca
     1381 atatccacct cataatcctg cttgaggtag gcaaaaatca gttccccgtt gattttgttg
     1441 tcctcaacca cgagaacctt gggtaaatta tgtggtttcc gcgatcttgc gattttgtga
     1501 ggttcctttt cttttaccgg agtttctttt ttgacatttt tttccgacgc gaggggcaat
     1561 agtatagtaa aaacagaacc tttgcccagt tggctttcca ccagaatatg cccgcccatc
     1621 atctctatca tttttttgct gatggataat cccaggccgc tgccgtcgta tcggcggttg
     1681 aggccctcgc tcacctgcct gaattcctga aatattttag aaaaattgtt agcatcgatt
     1741 cctatgccgg tatcttcaat ctttatgtgt gcaaattttt tgccctgatg ttctgaaagg
     1801 cctgttttta tggtaatact gcctttttcg gtataggcaa ttgcattgtt cactatattg
     1861 gtaattgatt gtatgagata gctctcattg aaaacggcaa caatatcatc ctcatactgc
     1921 ctgacaatgg agaggttttt ttgccgggct tttatttcaa gttgtccaag gggcatttcg
     1981 acgtacttgt ttacctcgct ggtaatgaga gtgatttgtt tgtttccggc ttcgagttgc
     2041 gataattgca gtatggaatt gatgatagac atcagtttgt tgcccgattc gataatggta
     2101 tcaaccatcg acagtgtttc gttgtctttt atcctgtttt taagtaatga tgcgaagcca
     2161 aggatgcctg tcatcggggt gcgcaaatcg tgattcatat tggtgatcag agaggtttta
     2221 aggcggtcgg attcttcggc ttttgccttt cccctgagga tttcttcttc agcctgcttt
     2281 ttataaatct gctgagctat ctgttccgaa acatacacca gcaggttttt ttcgttctcg
     2341 gtaaactgaa ttgctgtcga atagctttgt acggcaacta cgccaatgac tttattatga
     2401 gtgatgaggg gcacacccat ccaggtttcc gaataggcgc caatgagttc aatttcaccg
     2461 gaaagttcca gttgtttcag gagttcggag gtggtgacca cgggcttgcc atgtcgcagc
     2521 acatactcgg tgagcccccg tttcagacgg tgcgggctcg gggaggggtc tttttcatcc
     2581 acaaaataag ggaaagaaac aatgtcagag gcatcgtcat aaagagcaat gtagaagttt
     2641 ctggccggaa ggatttcgtt gatgatttta tgtatggatt tatacatatc gtacatattg
     2701 tcaacagtgt gggtcagctg cgaaatttta taaacggttt ctctggcttt ttccaggcgt
     2761 tttatttcgg taatgttgcg attgaagttc atgacggcgg gaattcctcc gtattctact
     2821 gttgagccat caaccagcat gtgtatttct ttgccttttc tgttcaggtg gatggtgttg
     2881 aaatctttgg taacaatatg gttttttatg ttttctatct gttgtttgtc cagatcaatt
     2941 tcccgggtaa tttgttccaa aggcatttca aaaaagtcgt ctattgtgta tccataggtc
     3001 tcggcggctt tttgattggc ggccattatt ttgtagtttt ccggattaat aatcagaatg
     3061 gctacactgg agtgctcaaa taaagccctg tactgtgctt cggaccgttg cagtgcctgc
     3121 ataaactttt tttgcccggt tatatcacgg acaacacggc tgatatagga agcattttgt
     3181 gttttaataa gaaaaatgga ataatccacg tacctttctt caccatcttt tctcaaaatt
     3241 tttccttcta tttttttaaa tttttcagga aatgcatctt cctgaaaagc gttcagggcg
     3301 ttttgtttga ttttttcata aaattcttta gtcttttttt tcggagtcat catttcgtat
     3361 tcgatatccc aatggtattt tcccaaagcc tgatcttttg taataccggt aatttccacc
     3421 aaagcattgt tccattcgat aattctcccg tgaatgtcgt ttatcgagat tccttcggtg
     3481 gcttgttcta tcacatttcg aaatttatct tcttcctgta ttaatgcctg ttcggttttt
     3541 tttt
//
LOCUS       DAOSFP010000246         3520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24725_length_3520_cov_3.192785, whole genome shotgun sequence.
ACCESSION   DAOSFP010000246 DAOSFP010000000
VERSION     DAOSFP010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24725_length_3520_cov_3.192785"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLI16_07570"
     CDS             complement(<1..967)
                     /locus_tag="PLI16_07570"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOH84455.1"
                     /translation="MKKFFLFLISFVVLSAFSSQVKAQLTVDTSLTVQQMVQNYLLGS
                     GVTVTNVTYTGKPGSVGAFNTGATPTNLGLASGLVLSNGQTNGTPAIGSPASNLADHN
                     NAGISDPLLENLVPPSTNIFDASVLEFDFVPFADTMKFRYVFASEEYPDYVSAMYADV
                     FGFFVTGPNPAGGNYTNQNIALIPGTSLPVTIANVNNGPGNSGPCNNCAYYIGNTGGM
                     TIVYNGFTTVLTAWCLVSPCASYHLKLAIGDAGDGWWDSSVFLEANSFSTNNIEVTAT
                     DAEICTGESTTLTAVGANTFLWMPDSIAGPVNTVSPTVTTTYTVIG"
     gene            complement(1161..1994)
                     /gene="folP"
                     /locus_tag="PLI16_07575"
     CDS             complement(1161..1994)
                     /gene="folP"
                     /locus_tag="PLI16_07575"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOH84456.1"
                     /translation="MNHYFLNCRGKLVSLEKPLIMGILNVTQDSFYDGGCYRDELSWL
                     THTEKMLADGADIIDVGCVSTRPGSRPITAIDETARAVEVLTSLKKSFPEIIISIDTY
                     RADVARASADAGADIINDISGGMMDVNMFPTVAELHIPYILMHMQGTPETMQMKPSYT
                     DVTKEILFFLSEKVCLLKRMGVNDIIIDPGFGFGKTIEQNFHLLKDIDFFKFLSHPLL
                     IGVSRKSMIWKVLKSSPEKALNGTSVLNTYALLKKAGIIRVHDVKEARECVELINFLS
                     V"
     gene            2052..2597
                     /locus_tag="PLI16_07580"
     CDS             2052..2597
                     /locus_tag="PLI16_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOH84457.1"
                     /translation="MNQTEKQYDVAIAACRELFVKKMNDYGTAWRILRPMSLTDQIFI
                     KAQRIRSIEEKGTQKISEGIFSEFIGIVNYSVMALIQLELNDFTNINLEIPKASELYD
                     KYVRLAKNLMMDKNHDYGEAWRDMRISSLTDIILMKIFRTKQIEDNQGLTEVSEGVDA
                     NYFDMINYAVFALIKINTEKK"
     gene            2617..>3520
                     /locus_tag="PLI16_07585"
     CDS             2617..>3520
                     /locus_tag="PLI16_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOH84458.1"
                     /translation="MSTNLIVIIILIAIAFADFLLYSFSKRLRKFSIHFARIVLGLVF
                     IFSGFVKAVDPLGSMYKFDDYFMAFGMDWLMPASLIMGFFLFCSEFIIGFCFLFNVKM
                     KFFSWIMILYMTFFTILTIILAVKNPVSECGCFGDAIILTNWETFYKNVGLLIFALIV
                     FWGRNIIKNRFPNVTQYGIMFIGFAIILWISVYCYRHLPLMDFMPWKVGNRISEKVVD
                     TPEIADINLVYKHKETGEIKKWTSKTLPYKDTAYFKQFEFVDQEKTVIQEFKPAPIHD
                     FMVDDVNKVNHNADIIGNPNYQFLL"
BASE COUNT         1057 a          757 c          689 g         1017 t
ORIGIN      
        1 ttccgataac agtataggtg gtagttaccg ttgggctgac cgtattgaca ggccccgcta
       61 ttgaatcggg catccataaa aaagtattgg caccaacagc ggtcagggta gtgctttctc
      121 cggtacagat ttcagcatcc gtagctgtca cctcaatatt atttgtagag aaactgtttg
      181 cttcgagaaa aacagaagaa tcccaccatc cgtcgccggc atcaccaatg gccagtttca
      241 gatgatatga cgcacagggt gaaacaaggc accaggcggt taataccgta gtaaatccat
      301 tataaacaat cgtcatgccg ccagtattgc cgatataata ggcgcaatta ttacatgggc
      361 cactgttgcc agggccgttg ttcacattgg caatggtaac cggtagtgat gtcccgggga
      421 tcagcgctat attctgattg gtataatttc cgccggcagg attgggtcct gtaacaaaaa
      481 agccaaatac atcggcatac atggcgctga cataatcggg gtactcctcc gaagcaaaca
      541 catacctgaa tttcattgtg tcggcaaacg gcacaaaatc aaattcaagc actgatgcat
      601 caaagatatt agttgatggc ggtacaaggt tttccagtaa gggatcgctg attcctgcat
      661 tgttatgatc tgcaagattg ctggctggag aacctatcgc cggtgttccg tttgtctgcc
      721 cgtttgagag caccaaaccg ctggcaagac ccaggtttgt gggtgtggcg cctgtattaa
      781 aagccccaac cgacccgggt ttgccggtat aggtcacatt ggtaactgtt acaccggaac
      841 ccagcagata attctgtacc atctgctgta ccgtaagaga tgtatccacc gtcagctgtg
      901 cttttacctg agaggaaaag gccgacagga caacaaaaga gattaaaaaa aggaaaaact
      961 ttttcatatg tttttggttt aataaatatt atttacgctc catgaatttt ttatacagaa
     1021 attttcgtta caccttatca ttaaatgtga ctttgcaaat tttcatccta tctatatatc
     1081 taatattatc aatatttttt ttcaaaaaac gttgtgaaga taaataaaaa ttaataatta
     1141 ccacaacccg aaaataattt ttatacggat aaaaaattaa taagttctac acactcccgg
     1201 gcttctttta catcatgcac acgaataatc cctgcttttt tcagcagggc gtaggtattc
     1261 agcacacttg tgccgttaag ggccttttcg ggcgaacttt tcaacacctt ccatatcatt
     1321 gattttctgg aaacaccaat taataaggga tgagataaaa atttaaaaaa gtcaatatcc
     1381 tttaacaaat gaaaattttg ttctatcgtt ttgccgaatc cgaaaccggg atctataata
     1441 atatcgttaa cccccattcg tttcaacagg caaacttttt ccgataaaaa aaacaggatt
     1501 tctttagtta catcagtata tgatggcttc atttgcatag tttccggggt cccctgcata
     1561 tgcatcaata tataaggtat gtgtaattca gcgaccgttg gaaacatatt tacatccatc
     1621 atgccccccg aaatatcatt aattatatcg gctccggcat ctgctgaagc acgggccaca
     1681 tcagccctgt aagtatcaat agaaataatt atctcaggaa agcttttttt taacgaggta
     1741 agcacttcaa cggcccgtgc tgtttcatct atggcagtaa tgggtctgga acccggcctg
     1801 gtggaaacac aacccacatc aataatatca gcgccatcgg ccagcatttt ttcggtatgt
     1861 gtcagccatg aaagttcatc acgataacag ccaccgtcat aaaaagaatc ctgagtaaca
     1921 ttcagtatcc ccatgatcag cggcttttcg agcgacacta atttgccccg gcaattcaga
     1981 aagtaatgat tcaccagttt ctaatttttt ttatctttgc acgttaatat tttacaaatg
     2041 taataaaata catgaaccag accgaaaaac aatacgacgt cgccattgct gcctgcaggg
     2101 aactttttgt aaaaaaaatg aacgattacg gcaccgcttg gaggatactg cgcccgatgt
     2161 cgcttaccga ccagattttt attaaggcac aacgcatccg aagcatagaa gaaaaaggaa
     2221 cgcaaaaaat ttctgaaggt attttctccg agtttatcgg cattgtcaat tactctgtca
     2281 tggccttgat acaactggaa ctgaacgatt tcaccaacat caatttggaa ataccaaagg
     2341 cctcggagct gtacgacaaa tatgtccgtc ttgccaaaaa cctgatgatg gataaaaacc
     2401 acgactatgg cgaggcctgg cgcgatatgc gcattagttc gctcaccgat attatcctga
     2461 tgaaaatttt cagaaccaaa caaattgaag ataatcaggg attgaccgaa gtatcggaag
     2521 gtgtggatgc caattatttt gatatgatca actacgccgt attcgcgctg attaaaatca
     2581 acacagaaaa aaaataacta ctaaatcaaa caagttatga gtaccaatct tatagtaata
     2641 ataattctga tagccattgc ttttgcagat ttcctgttat actctttttc aaagcgcctg
     2701 cgcaagtttt ccatacattt tgcccgcatt gtcctgggat tggtatttat tttttcgggc
     2761 tttgtaaaag ccgttgaccc gctggggtcc atgtataaat ttgatgatta ttttatggca
     2821 ttcggtatgg attggctgat gccggcctcg ctgatcatgg gtttttttct tttttgttcg
     2881 gagtttatca ttggattttg tttcctgttc aatgtcaaaa tgaaattttt ttcatggata
     2941 atgatattgt acatgacctt tttcaccata ctcaccatta tcctggcagt taaaaacccc
     3001 gtgtcggaat gcggctgttt tggcgatgcc ataatactta ccaactggga aactttttat
     3061 aaaaatgtcg gcctgctgat tttcgccctt atcgtttttt ggggaagaaa tattatcaaa
     3121 aaccggttcc ccaatgtgac ccaatacgga ataatgttta tcgggtttgc catcatactg
     3181 tggatttcgg tgtattgcta ccgccacctg cccctgatgg acttcatgcc ctggaaagta
     3241 ggcaacagaa tttcggaaaa agtagttgac actcccgaaa tcgccgatat taacttagtg
     3301 tataaacaca aagaaaccgg cgaaatcaaa aaatggacat ccaaaacgct tccttataag
     3361 gatacggcct attttaaaca atttgaattt gttgaccagg agaaaaccgt catccaggaa
     3421 ttcaaacccg cgcccataca cgactttatg gttgacgatg tgaataaagt caatcataac
     3481 gctgatatca tcggcaaccc caactaccag ttcctgctta
//
LOCUS       DAOSFP010000247         3514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24784_length_3514_cov_4.507372, whole genome shotgun sequence.
ACCESSION   DAOSFP010000247 DAOSFP010000000
VERSION     DAOSFP010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24784_length_3514_cov_4.507372"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLI16_07590"
     CDS             <1..359
                     /locus_tag="PLI16_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84459.1"
                     /translation="EVPVEDTVSLYNVTLAIRHTSYYAYANIRVNMMINYPTGDMRVK
                     DYNILVRNTDGSFLGDGAGDLWDVTHPALEGVSFPFKGIYVFEVQNVMPLMTTPDIMD
                     VGLIVRKSKKKIETTE"
     gene            343..2151
                     /gene="uvrC"
                     /locus_tag="PLI16_07595"
     CDS             343..2151
                     /gene="uvrC"
                     /locus_tag="PLI16_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOH84460.1"
                     /translation="MKQLNDHIKNILAALPDKPGVYQFFNSTGTIIYVGKAKKLNKRV
                     RSYFKDESSMTGKLTVLVKKIADIKYIVVDNEYEALLLENNLIKKYQPRYNVLLKDDK
                     TYPWICIKKERFPRIFYTRNIIRDGSEYFGPYASGRMMNTLLELIFQIFQVRTCNYNL
                     TEENIKRRKYKVCLKYHIGNCKGPCDGHQNEEDYNETIAGIKEIIKGNINAVQQQLKK
                     AMSTFADAYEFEKAQIVKEKIELLQRYQSKSTVVNPAISNVDVFSIIDDDNTAYINFL
                     KVVDGAIVQAHTVELRKHLDETPVQLLEYAITDLRDRMKSNSAEIIVPIALSIDFPGT
                     KITVPQKGDKKKLLDLSVNNAKYYKLDKEKQKELVDPDRHVTRIMEQAMKDLHLNELP
                     DHIECFDNSNTQGSFPVASMVVFKHGRPEKSEYRHYNIKTVEGPNDFASMEEVVFRRY
                     KRLLDENKALPKLIVVDGGKGQLSSALHSLEKLGLRGKIAIIGIAKKLEEIYFPGDSI
                     PLYIDKKSETLKLIQQLRDEAHRFGITHHRQKRQKATVKSVLSDIEGIGYETTQKLLR
                     KFKSVANIKKASFEDLQPVVGAQKAKIITEYFNKKD"
     gene            2209..3456
                     /locus_tag="PLI16_07600"
     CDS             2209..3456
                     /locus_tag="PLI16_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOH84461.1"
                     /translation="MKTLNLFFTAGLVSLVLLCACKTNKNNMSTTSTPSEENSPAFSI
                     AESNNRFAFDLYKNISTKPENMFYSPYSISTAMAMTYTGARGNTEKQMGAVMHFPPNS
                     MDFNNQNNTGLKEINALNDGNALNTYTANSIWAQKDFKFKENFIGALKDSYLSSLHTV
                     DFIKETEQSRLLINKWVETETKEKIKDLIQPGLIDYLTRMVLVNAIYFKADWETAFDG
                     TLTKKMPFFQDETKQESCDFMFAERDFKFYEQEDGLKAIEVPYSKGKMSMLVILPKDI
                     NGIFGLTKETGLDWYNKINSGLAMKKVKLWLPKFKTTSEFELSEMLQQMGMKDAFTDN
                     ADFSGMTGKKDLKISKVIHKAFVEVNEAGTEAAAATAVVMRVKSMPVNQPEFKADHPF
                     LFIIKENTQNRILFMGNIFNPAK"
BASE COUNT         1184 a          816 c          715 g          799 t
ORIGIN      
        1 ttgaagtgcc tgttgaagac actgtgagtc tttacaacgt aacgctggct atccggcaca
       61 cttcatatta cgcctatgcc aacatccgcg taaacatgat gatcaactat cctacggggg
      121 atatgcgcgt taaggactac aacatccttg tcagaaatac cgatggttct tttcttggcg
      181 acggcgccgg cgacctgtgg gatgttactc acccggcact tgaaggggta agctttcctt
      241 ttaagggcat ctatgttttc gaagtccaga atgtgatgcc cctgatgaca acgcctgata
      301 tcatggacgt gggccttata gtgcgaaaat cgaaaaaaaa gattgaaaca actgaatgac
      361 catataaaaa acatcctcgc cgcactgccc gacaaacccg gtgtatatca gtttttcaac
      421 agtaccggga ctatcatcta tgtgggcaaa gccaaaaaac tgaacaagcg cgtcaggagt
      481 tactttaagg atgagtcgtc gatgacgggc aaactcactg tgctcgtaaa aaaaattgcc
      541 gacataaaat acattgtagt tgacaatgaa tacgaagccc tgctgctcga aaacaacctg
      601 ataaaaaaat atcagccccg ctataatgta ttactgaaag acgataagac ctatccctgg
      661 atatgcatca aaaaggaacg atttccacgg atattttata cccgcaacat tatcagggac
      721 ggctctgaat actttggacc atatgcttcc gggcgcatga tgaatacctt gctggagctg
      781 atatttcaaa ttttccaggt gcgtacctgc aactacaacc ttaccgaaga aaacataaaa
      841 cgccgaaagt acaaggtgtg tctcaaatat cacattggca actgtaaggg cccctgcgac
      901 ggtcatcaaa acgaagaaga ctacaacgag accatcgccg gcatcaaaga gattatcaag
      961 gggaacatca atgccgttca acagcagcta aaaaaagcca tgagcacttt tgccgatgca
     1021 tatgaatttg aaaaagcaca gattgtaaaa gaaaaaatag aattactcca aagataccag
     1081 agcaaatcta ccgtggtaaa tcccgccatc agcaatgtgg atgtgttcag catcattgat
     1141 gacgataata cagcttatat caatttttta aaagtggtgg atggcgccat cgtccaggcc
     1201 cataccgtgg agctgcgcaa acaccttgac gaaacaccgg ttcagttact cgaatacgcc
     1261 atcaccgacc tgcgggaccg aatgaaaagc aactccgccg aaataattgt ccctattgct
     1321 ttaagcattg atttcccggg aacaaaaata accgtcccgc aaaaaggcga caaaaaaaag
     1381 ctgctcgacc tatcggtaaa caatgcaaaa tactacaagc tcgacaaaga aaaacaaaaa
     1441 gaacttgtcg atcccgacag gcatgttacc cgcatcatgg agcaggcaat gaaagatttg
     1501 cacctgaacg aactgcccga ccatattgag tgttttgaca attcgaatac gcagggaagt
     1561 tttccggtag cctcgatggt ggtttttaaa catggccgcc ccgaaaaaag cgaataccga
     1621 cattataaca tcaaaaccgt ggaaggcccc aacgattttg cttccatgga agaggtggtc
     1681 ttcagacgtt ataaaagact gctcgacgag aacaaagccc tgcccaaact catcgtggtg
     1741 gacggcggaa aagggcaact aagctcggcc ctccacagcc ttgaaaaact gggcttacgc
     1801 ggaaaaattg ctatcatagg catcgccaaa aaactggaag aaatatattt tcccggagac
     1861 agcatcccgc tgtatatcga caaaaaatca gaaacgctga aactgataca acaactgcgc
     1921 gatgaggcac accgttttgg tatcacgcac caccggcaaa aaagacaaaa agcaaccgtc
     1981 aagtccgttt tatcagatat cgaaggcatt ggatacgaaa ccacacaaaa acttttacgg
     2041 aaattcaaat cggttgctaa tattaaaaaa gcatcgttcg aagacctgca gccggtggta
     2101 ggagcacaaa aagcaaaaat tattactgaa tatttcaata aaaaggattg atatttgcag
     2161 tcaccgaata ttgatgttac tttgtacaat attttaacat aaccggatat gaaaacattg
     2221 aatctatttt ttacggcagg cctggtatcc cttgttttgc tgtgtgcctg caaaaccaac
     2281 aaaaacaata tgagcaccac ctccacccca tcagaagaaa actcccctgc cttcagcatc
     2341 gccgagagca acaaccggtt tgcctttgac ctttacaaaa atatcagtac caagcccgaa
     2401 aacatgtttt attcgcccta cagcatttcc acggccatgg ccatgactta tacgggagcc
     2461 cgcggaaaca ccgaaaaaca aatgggtgcc gtcatgcact ttccaccgaa cagcatggat
     2521 tttaacaacc aaaacaacac cgggctcaaa gaaatcaacg cactgaacga cggcaacgcg
     2581 ctaaacactt acaccgcaaa ctctatctgg gcccaaaaag attttaaatt caaagaaaat
     2641 ttcatcgggg cgctaaaaga cagctacctg tcatcattgc ataccgtgga ttttattaaa
     2701 gaaacggaac aaagccgctt actgatcaat aagtgggtgg aaacagaaac caaagaaaaa
     2761 atcaaagacc tgatacaacc tggccttatc gattatctta ccagaatggt tttggtgaat
     2821 gctatttatt ttaaagccga ttgggaaaca gcttttgatg gaacgcttac caaaaaaatg
     2881 ccttttttcc aggatgaaac caaacaggag tcatgcgatt ttatgtttgc tgaaagagat
     2941 ttcaaatttt acgaacagga agacggactc aaagccattg aagtacccta ttctaaagga
     3001 aaaatgtcga tgcttgtgat actacccaaa gatataaacg gcatcttcgg tctgacaaaa
     3061 gaaaccggcc tcgactggta caataagatt aacagcggcc tggctatgaa aaaagttaaa
     3121 ctttggctgc caaaattcaa gactacttct gaatttgaat taagtgaaat gcttcaacaa
     3181 atgggcatga aggatgcctt taccgataat gctgattttt cgggcatgac cggcaaaaag
     3241 gatcttaaaa tcagcaaagt aattcacaaa gcttttgttg aagtcaacga agccggcacc
     3301 gaagctgccg ctgccacagc cgtggtgatg cgggtaaaat ccatgcctgt gaaccaacct
     3361 gaatttaagg ctgatcatcc cttcctattc atcatcaaag aaaacacgca aaaccgcatc
     3421 ctatttatgg gcaacatttt taatccggca aaataacacc atggcggagt gcacacccat
     3481 acaaaccacc ggctggagtg actacgagct tatc
//
LOCUS       DAOSFP010000248         3507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24857_length_3507_cov_4.351390, whole genome shotgun sequence.
ACCESSION   DAOSFP010000248 DAOSFP010000000
VERSION     DAOSFP010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24857_length_3507_cov_4.351390"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..958
                     /locus_tag="PLI16_07605"
     CDS             113..958
                     /locus_tag="PLI16_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOH84462.1"
                     /translation="MKQIVVAIDFSKHSIHALELAITIANVIRAHIMMVWVDKPDSDD
                     SIFDNADHEHRQEAKQRLEDMVTKYSKQLKKGKLTYKLRKGKIYKEIANQAKYNDAYL
                     IIAGSHGVSGFESFWIGSNASKIVTYSPCPVITLRESYPLKKSIQKVVLPIDNSTSTR
                     QKVPFAMDFAKCFGSEIHVLELQSTKLKAVRNKVSSYAAQVSDYLTENKVKFIVKTIE
                     ADNITNATIKYAEEVKADLIIIMTEQEQELSNIWLGPYAQQMVNNSPIPVMSIHPKEL
                     DYLAK"
     gene            969..1388
                     /locus_tag="PLI16_07610"
     CDS             969..1388
                     /locus_tag="PLI16_07610"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOH84463.1"
                     /translation="MRIRFIRIVFLGVFLLASRAVFSQDNVLRQKISVIEDPQIVSLV
                     QKHIYLNEKQKITGWRVQIFFESGNNSKSRAHAKKGLFMTLFPGTGVYLMFQSPYYKV
                     RVGDFRSRMDAEGFKQKIINDFPDSFVVKDEINFPEL"
     gene            complement(1400..2944)
                     /locus_tag="PLI16_07615"
     CDS             complement(1400..2944)
                     /locus_tag="PLI16_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84464.1"
                     /translation="MKKILSLLVFVLPAIFVSAQNDVKWTRIDSPKEKYDFKYFISTS
                     PEASKAVFTIPHNKPFQQTKHNIFIRNYNSDLSNFSETGIPPESPVTLFLAGFRDYTV
                     ISGSMDENKNPMAQFAKENMIVIADDKMNPLVVKTFPLHGKKNKFTGSPELYLSHDST
                     HLIVVNNEILAFDKPSLKEPPKMTYIDVYDKSLNHVWSDSVNFNTIFGEKTDINNYSM
                     DFVGGKLILTASCPSDGMKKIKATVYLVAFDKPQSYKIIWNQIFPYENLAYRTVVGPQ
                     GNIIISGANMMGSNAPFGQAKKQLFFLNKDLKNTEAEPVFKSYELDKTFVAKYPEYKM
                     FLAESLFGPFDLFLLGDRVVYASEHRYYSTSTSSSGSSSTTYYFKHITLLCFDNEGQI
                     EWLNMINKSVASKQSFDDHFCNGFVSGNNLCFFYYDYADNIAAAKFIEKPRFVGEDKL
                     WVTQAIVSPDGDIKKTMITNIGAHDIRANLSATQQVNDHRFILVGTGTSLSSLGGYFG
                     IYELDK"
     gene            complement(3009..>3507)
                     /locus_tag="PLI16_07620"
     CDS             complement(3009..>3507)
                     /locus_tag="PLI16_07620"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOH84465.1"
                     /translation="RLYTQPQDVAYAYEQLKKVSNQFTIAASFGNVHGVYKPGNVKLE
                     PVILKNSQQYIQEKYGTLPNPVNFVFHGGSGSEPEKIREAISYGVVKMNIDTDTQWAY
                     WEGVLNYYKKNEAYLQGQLGNPEGPEKPNKKFYDPRAWLRNGEKSVVERLKIAFTDLN
                     AINRN"
BASE COUNT         1025 a          691 c          761 g         1030 t
ORIGIN      
        1 caaaaggcca ttttcattgt ttttatgatt cctgaaattt atttttaaaa aagacatttg
       61 ctaaatgtat tttttcattt aatttagcaa aaaaaacaca aaagatatcc atatgaaaca
      121 gattgtagtt gctatagact tttcaaagca ttccatccat gcgttggaat tagccatcac
      181 cattgccaat gtgatacgcg ctcatattat gatggtgtgg gttgacaaac ccgatagtga
      241 tgattccatt tttgataatg cagaccatga acaccggcag gaggccaaac agcggctcga
      301 ggatatggta acaaagtatt caaaacaact gaaaaaagga aaactcacct acaagctcag
      361 gaagggcaag atatacaaag agattgccaa tcaggcaaaa tataacgatg cgtaccttat
      421 tattgccgga tcgcacggtg tgtcgggttt tgaatcgttc tggataggaa gcaatgccag
      481 caaaattgtc acctattcgc cctgtccggt tatcaccctt agagagtcgt acccattaaa
      541 gaaaagcata caaaaagtgg tgctgcctat tgataattcc acttccacca ggcaaaaagt
      601 tccctttgcc atggattttg ccaaatgctt tggctccgaa attcatgtgc tggagttgca
      661 gtccaccaag ctaaaagcag tgcgaaacaa agtatcgagt tatgctgccc aggtgtcgga
      721 ttatcttacc gaaaataaag taaaatttat tgtaaaaact attgaggcgg acaatattac
      781 caatgccacc atcaagtatg ccgaagaagt taaagccgat ctgattatca tcatgaccga
      841 acaagagcag gaactgtcca acatctggct cggcccttat gcacagcaga tggtaaacaa
      901 ctccccgatt cccgtgatga gcatccaccc caaagaactg gactacctcg cgaagtagta
      961 ataaaaaaat gagaatcaga tttatcagaa tagtatttct gggtgtattt ttattagcat
     1021 ccagggctgt tttttcgcag gataatgtgt tgaggcaaaa gatttcggtg atagaggatc
     1081 cgcagattgt ttcgctggtt cagaaacaca tttacctgaa cgaaaaacaa aaaattaccg
     1141 gttggcgggt tcagattttt ttcgagtcgg gaaataattc aaaaagccgt gcccacgcca
     1201 aaaaaggatt gtttatgaca ctttttcccg gtacgggtgt ttacctgatg tttcagtcgc
     1261 cgtattacaa agtccgggtg ggtgattttc gttcgcggat ggatgcagaa ggcttcaaac
     1321 aaaaaatcat caatgatttt cccgactcat ttgtggtaaa agatgagata aattttcctg
     1381 agttatagtt ttttctttct tacttatcca actcgtaaat cccgaaatag cctcccaggg
     1441 aagacaatga agtgccggtg ccaaccagaa taaaacgatg gtcgtttact tgttgtgttg
     1501 ctgacagatt tgccctgata tcatgggcgc cgatattggt tatcatcgtt tttttaatgt
     1561 cgccatccgg gctgacgatc gcctgtgtta cccacaattt atcttcacca acaaaccgcg
     1621 gcttttcgat gaatttggcg gcggcaatat tatcggcata atcatagtaa aaaaagcaaa
     1681 gattgtttcc gctaacgaaa ccgttgcaaa aatggtcatc aaaggattgt ttgctggcca
     1741 ccgacttatt gatcatattc agccattcga tttgcccttc gttatcgaaa cacagcagtg
     1801 taatatgttt aaaataatag gtagtggaac tgctgcccga tgaagaggtg cttgtggagt
     1861 aataacggtg ttctgaagca taaaccactc tatcacctaa cagaaacagg tcaaaaggcc
     1921 cgaaaaggct ttctgccaga aacatcttat attcaggata tttggcaacg aatgttttat
     1981 ccagttcata gcttttaaaa accggctctg cttcagtatt tttcaaatcc ttattcagaa
     2041 aaaacaattg tttttttgcc tggccgaaag gggcgttgga ccccatcata ttggcgccgc
     2101 tgataattat attgccctga ggacctacca cggtgcggta tgccagattt tcataaggga
     2161 aaatttgatt ccagataatc ttataggatt gcggcttatc aaaggctacc agataaaccg
     2221 tggctttgat ctttttcata ccgtcggaag gacatgaagc tgtgaggatg agtttgccgc
     2281 ccacaaaatc catcgaataa ttattgatgt cggttttttc gccgaaaata gtgttgaagt
     2341 taacagagtc gctccagaca tgattaaggc ttttatcata cacatcaata taggtcattt
     2401 ttggcggttc tttcaggctg ggtttgtcaa aagccagaat ttcattgttc accactatca
     2461 ggtgggtaga gtcgtgcgaa agatataatt ccggcgaacc ggtaaatttg ttttttttgc
     2521 catgaagcgg gaatgttttt accaccaacg ggttcatttt gtcgtcggca ataactatca
     2581 tgttttcctt tgcgaattga gccatggggt ttttattttc gtccatggag ccgctgatta
     2641 cggtgtaatc gcgaaatccg gcaagaaaca aagtaaccgg agattcaggc ggtatgcccg
     2701 tttcagaaaa gttactcaga tcgctgttgt aattgcggat aaaaatattg tgtttggtct
     2761 gctgaaaagg tttgttgtga gggatggtga acacggcctt gctggcttcc ggcgaagtgc
     2821 tgatgaaata tttgaaatca tatttttcct taggactgtc aatgcgtgtc catttgacat
     2881 cgttttgtgc cgaaacaaaa attgccggca atacaaatac taataacgag agtatttttt
     2941 tcataaagga taaaaattaa tattatgaga aaaaaataag gtagagagcg acaaaaaaga
     3001 aattgccttt agtttctgtt aatggcattc agatctgtaa aggcgatttt aagtctttcc
     3061 accacagatt tttcgccatt acgcagccag gcccgcgggt cgtagaattt tttgttaggt
     3121 ttttcaggcc cttcggggtt gccaagctgg ccctgaaggt aggcttcatt tttcttgtaa
     3181 tagtttaaaa cgccttccca ataagcccat tgagtatcgg tgtcaatgtt cattttgaca
     3241 acgccataag aaatggcttc gcggattttt tccggttcgg agcctgagcc gccatggaaa
     3301 acaaagttta cgggattggg cagggtgcca tatttttcct gaatatactg ctgagaattt
     3361 tttagaatga caggttccag ttttacattg cccggtttat acaccccgtg gacattacca
     3421 aaggatgcgg caatagtaaa ctgattgctg acttttttca gttgttcata ggcataggcc
     3481 acgtcctgcg gctgggtata aagcctt
//
LOCUS       DAOSFP010000249         3497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24937_length_3497_cov_3.180128, whole genome shotgun sequence.
ACCESSION   DAOSFP010000249 DAOSFP010000000
VERSION     DAOSFP010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24937_length_3497_cov_3.180128"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="PLI16_07625"
     CDS             complement(<1..408)
                     /locus_tag="PLI16_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HOH84466.1"
                     /translation="MKLHREGTRIILFSALLLLLINFPVFMLLDDYLWFKIFILTASL
                     VCYVIILRFFRVPAREYVQDPELIFSGADGEVVAIEEIDEEKYLNEKRIQVSVFMSLN
                     NVHINFYPVSGEVIKTEDYYGRYFVARKPKSSYM"
     gene            complement(411..1235)
                     /locus_tag="PLI16_07630"
     CDS             complement(411..1235)
                     /locus_tag="PLI16_07630"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOH84467.1"
                     /translation="MKKLITRTITGLVFITIVIGAICWSVYSFMLVFLVVALLGIYEF
                     YKLALLKKIQPQFLNGLVFGGFLFVSISLYAHNVFDFGWVLLSLPIAIIIPVSELFRK
                     EGQPFTNIAYTVLGGLYVALPLALLNFFYSPFELRGGFNTHILLGFFIITWVADTAAY
                     LVGSAIGKHKLFERISPGKTWEGSVGSVVFGVAAAYVFSLIFKDIQFLHWAIISVIIV
                     VTGTLGDLAESMLKRSVNVKDSGTIMPGHGGILDRFDGVFFAAVFVFIYIYFFIAN"
     gene            complement(1232..1435)
                     /locus_tag="PLI16_07635"
     CDS             complement(1232..1435)
                     /locus_tag="PLI16_07635"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOH84468.1"
                     /translation="MEKDWVLVFETNKDIEAEMVRGMLEENDISVFVINKKDSAYLFG
                     NIELYVLPENLLVAKTYIKDFNL"
     gene            complement(1439..2086)
                     /locus_tag="PLI16_07640"
     CDS             complement(1439..2086)
                     /locus_tag="PLI16_07640"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOH84469.1"
                     /translation="MEEYSSREKVLKNVRNAGIHQAECPFSEIDFSQNVFKVSEDPLE
                     VAFAKAFVAIAGKFVYCADKKEFVDSINAFAKENKWDNMYCLEPSIQSLLVEAAVPFS
                     SDPDTLTEAVQGITSCEYLVSRLGSIMVSSVQASGRRMIVYPDVHIVMAYASQIVPDL
                     PEAFEALFKKYKNNLPSLISVISGPSRTADIEKTLVMGAHGPRDLYLFLIDDINQ"
     gene            complement(2112..>3497)
                     /gene="ftsH"
                     /locus_tag="PLI16_07645"
     CDS             complement(2112..>3497)
                     /gene="ftsH"
                     /locus_tag="PLI16_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOH84470.1"
                     /translation="AVAGEAKVPFFSLSGSDFVEMFVGVGASRVRDLFKTAKEKAPCI
                     VFIDEIDAIGRARGKNPMTGANDERENTLNQLLTEMDGFSSNAGVIILAATNRADILD
                     RALLRAGRFDRQIHVELPDLEERTAIFKVHMKPLTLSKDIDIGFLSRQTPGFSGADIA
                     NVCNESALIAARKNKKVIEKQDFLDAIDRIVGGLEKRSKIISMEEKKVIAFHEAGHAA
                     ISWLVEHASPLVKVTIIPRGKALGAAWYLPEERQITFTEQMLDEMCAILGGRASEEVN
                     FGKISTGALSDLERTTKQAYAMVMYYGLNKKIGNLSYYDSSGQQEYALSKPYSETTAQ
                     TIDEEVSKMVDAAYTRAVKIIRDNKEKVTELANLLLEKEVIFREDVEKIFGKRPFDDG
                     HHIDQDARDIEFTLRKGKPEGAEKKQEQDTSETKSEPGENAGQKAAGPEDIENKEDKE
                     NPQPTTDNTEN"
BASE COUNT          945 a          831 c          770 g          951 t
ORIGIN      
        1 catataggaa gatttcggtt tacgagccac aaagtagcgg ccataatagt cttcggtttt
       61 tatcacctcg ccgctgacag ggtaaaaatt aatatgaaca ttattaagtg acataaatac
      121 agatacttgt atgcgttttt cgttcaggta tttttcctca tcaatttctt cgatagccac
      181 tacctcgccg tcggcgccgg aaaatatcag ttcaggatct tgcacatatt cccttgccgg
      241 caccctgaaa aaacgcagta tgatcacata acacaccagg gaagccgtca atatgaaaat
      301 tttgaaccac aaataatcgt ccagtagcat aaaaaccgga aagtttataa gcaacagcaa
      361 caacgctgaa aaaagaatta ttcgcgtacc ttctctatgt aatttcataa ttaattggca
      421 ataaaaaagt aaatataaat gaaaacaaaa accgcggcaa agaacacccc gtcgaaacgg
      481 tcgaggatac cgccgtggcc cggcatgatg gtgccggaat ccttcacatt cacgctgcgt
      541 tttagcatgg attcggccag atcgcccagt gtgcctgtca ctacaatgat aaccgaaatg
      601 atggcccaat gaaggaactg tatatccttg aaaatcagcg aaaagacata agcggctgcc
      661 acaccgaaaa caaccgatcc cacggaacct tcccaggttt taccgggtga gatacgttca
      721 aacagcttgt gttttccaat ggctgaacca accaggtaag ctgccgtatc ggcaacccag
      781 gtgataataa aaaagccgag gaggatgtgg gtattaaacc cgcctctcaa ctcgaaagga
      841 ctgtagaaaa aattcagcag cgccagtggc agcgcgacat aaagtccccc caggaccgta
      901 taagcaatat tggtaaacgg ctgtccttct ttccggaaaa gttcagaaac aggaatgatg
      961 atggcaatag gcaaacttaa caatacccag ccaaaatcaa aaacgttatg agcatataat
     1021 gagatactta caaacaaaaa accgccaaat accaggccgt ttaaaaactg aggctggatt
     1081 tttttcagca gggcaagttt ataaaactca tatatgccga gcagcgcaac cacaagaaat
     1141 accagcataa aagaataaac actccagcaa atggcgccta ttacaatagt aataaacaca
     1201 aggccggtga tggtacgggt gataagcttt ttcacaagtt aaaatccttt atataagttt
     1261 ttgccaccaa cagattttcg ggcaagacat acagttcgat attaccgaaa aggtatgccg
     1321 aatccttttt atttatgaca aaaacactga tgtcattttc ctcaagcatc cccctcacca
     1381 tttcggcttc aatatccttg ttcgtttcaa aaacgagtac ccaatctttt tccatagttt
     1441 attgatttat gtcgtcaatt aaaaaaagat ataggtcgcg ggggccatga gcgcccatta
     1501 ccagggtttt ttcgatatcg gcggtacggc tgggcccgga tatcactgat atcagcgaag
     1561 gaagattatt tttatatttt ttgaacagcg cctcgaaagc ttcgggcagg tcgggcacaa
     1621 tctgcgaggc gtaggccata actatgtgca catcgggata tactatcatg cggcggccgg
     1681 aggcctgtac cgatgacacc atgatgctgc ccagcctgga gacaaggtat tcgcaggaag
     1741 taattccttg tacagcttcg gtgagcgtat ccgggtcaga gctgaaaggc acggctgctt
     1801 ctaccagcag gctttgtatg gatggctcca ggcaatacat attgtcccat ttgttttcct
     1861 ttgcaaaagc gttgatgcta tccacaaact ctttcttatc ggcgcaatac acaaatttgc
     1921 cggcaattgc aacaaaagct tttgcaaaag cgacttccaa ggggtcctca gacactttaa
     1981 aaacattctg ggaaaagtca atttccgaaa aagggcattc cgcctggtgt attccggcgt
     2041 ttcgcacatt tttcagcact ttttcccgtg agctgtattc ttccataaag gtctgtcagg
     2101 tattaaaata actaattttc agtattatca gttgttggtt gtgggttttc tttatcttct
     2161 ttattttcaa tatcttcagg accggcagcc ttttgtccgg cattttcccc cggttcggat
     2221 tttgtttctg aagtatcttg ctcctgcttt ttttcagcgc cttcgggttt tcctttacgc
     2281 aaagtaaact caatatctct ggcatcctgg tcaatatgat gtccgtcatc gaaaggcctt
     2341 ttgccgaata tcttttcgac atcttcgcgg aaaatcacct ctttctcgag tagcagatta
     2401 gccagttcag ttaccttttc tttgttgtcc cggataatct tcacagccct ggtataggca
     2461 gcatccacca ttttgcttac ttcctcgtcg atggtttgcg cggttgtttc gctgtagggt
     2521 ttggaaaggg catattcctg ctgtcctgat gaatcgtaat agcttaagtt gccgattttt
     2581 ttgtttagtc cgtaatacat caccatggca tacgcctgct tcgtggttct ttccaggtcg
     2641 ctgagcgcgc cggtggatat tttcccgaaa ttgacttctt cggaagctct gccgccgagg
     2701 atagcgcaca tctcgtcgag catttgttcg gtaaaagtga tttgtctttc ttcgggcagg
     2761 taccaggcgg cgcccagggc tttgccgcgc gggataatag taactttaac cagtggcgaa
     2821 gcatgttcca ccagccagct tatagcggca tgaccggcct catgaaaagc gatgactttc
     2881 ttctcttcca tggagatgat tttgcttcgt ttttccagac cgccgacgat acggtcgatg
     2941 gcatcgagaa aatcctgctt ttcgataact tttttgtttt tgcgggccgc tatcagcgcc
     3001 gactcattac atacgttggc aatatcggca cctgagaaac ccggtgtctg acgcgagagg
     3061 aatccgatat cgatatcttt cgacaaggtc agcggtttca tatgtacctt gaaaatggct
     3121 gttctttctt ccagatcagg gagttcaacg tgtatctgcc tgtcgaaacg cccggcacgc
     3181 agcaaggccc tgtcgaggat gtcggcacgg ttggtggccg ccaggataat cacaccggca
     3241 ttacttgaaa agccgtccat ttcggtgagc aactgattga gggtattttc tctctcgtcg
     3301 ttggcgcccg tcatgggatt tttgccacgg gcacggccaa tggcatcaat ttcgtcgata
     3361 aaaactatgc aaggtgcttt ttctttggcg gttttaaaca agtcgcgtac gcgggaggcg
     3421 cccacgccaa caaacatctc cacaaagtcg gaacctgaaa gtgagaagaa aggcacctta
     3481 gcttcgccgg ccaccgc
//
LOCUS       DAOSFP010000250         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24951_length_3495_cov_4.002616, whole genome shotgun sequence.
ACCESSION   DAOSFP010000250 DAOSFP010000000
VERSION     DAOSFP010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24951_length_3495_cov_4.002616"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLI16_07650"
     CDS             <1..479
                     /locus_tag="PLI16_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84471.1"
                     /translation="ITMHFNAFDITPRDYVRVTDLEDNTLIAELTGSVIPDDIICNSG
                     QILVQLKTFDKVMAQGFSISYYNSSNLNEIPHTEFSVSPNPAKDLLTLTFQNGQQEEY
                     ALKIFSVEGRLLFEKAFRLSPGQQNQVIDVSMLQPGFYLLHIASGNVNSTKKIIIE"
     gene            488..2590
                     /locus_tag="PLI16_07655"
     CDS             488..2590
                     /locus_tag="PLI16_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH84472.1"
                     /translation="MKKIHIYFSLIGLFLANISLTFAEKIDVKTAQTLATNFYYEKCL
                     LFGYDLKQPEELTLSFTKTKNSEEMYYIFSSQNGYIIVAAEDDAYPVLAYSFEGPYTG
                     KNISPEFGYWMSIYEDQIIHIRKNSMKADKKISDTWNFYLTDFICDEQLKNGTKSVAP
                     LLMSTWDQGTYYNYLCPPDPAGAGGHVYSGCVATAMAQVMYYYRYPVQGTGSHGYYSN
                     YGYLSANFGATTYQWNEMQNNIGGKFNYSMALIQLHCGIAIDMDYSPDGSGASMYDAA
                     SAMKSYFGYSASTQLYRKDDYTETQWKNMLIDDLDNKMPIQYAGYGESGHAFVCDGYQ
                     GTDFFHFNWGWSGYYNGYFYLNNLNPGYTFSNWQQAILNSYPATAFPQQCSNTTLVQS
                     TCGTVEDGSSPANDYENNKDCMWLIAPTDPVDYIRITFERLNTEAGNDFITIYDGETT
                     SDPVLGVFSGSTIPSDVLSTGNKVLVRFTTNSSVVADGWLLTYNGKITSFCSGVTELT
                     APSGVISDGSGTYNYNNSTLCHWRISPPDAGTISITFNNLDLAQDNDFVKIYDDNSDT
                     ELMTLHAGDLPPTFTTYTGKVLLLFFTSVMDNAGGWELSYTSAPLSVSENPEAFVSIA
                     PNPASEYVKLQAFLPQATTVDLAICNNLGSVVLSRQLSSTDGRFDEAVDVSDLSQGIY
                     FLKLAADSFQQTQKLIIR"
     gene            2678..>3495
                     /gene="aroC"
                     /locus_tag="PLI16_07660"
     CDS             2678..>3495
                     /gene="aroC"
                     /locus_tag="PLI16_07660"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOH84473.1"
                     /translation="MSNTFGELFRLSIFGDSHGPSMGGIIDGCPSGLVIDENKILADL
                     QRRQSGTTSFASARKEPDKFTLLSGISQGKTTGGPIGFLIPNKDSKRADYQHLDDIFR
                     PSHADFTYYAKYGTMESAGKLHASGRIFVPVVLAGSIAKQILALHGIRVFAYVRQIGT
                     ISTPFSYEEIDPETIENSPVRCPDKKATKEMLHLLDKLKKLGDSTGGIISGVVEGCPP
                     GLGDPVFGKLQAKLAQAMFSINAVKGFEYGSGFAAAGMKGSEHNDLFVVKQNRLR"
BASE COUNT          969 a          851 c          766 g          909 t
ORIGIN      
        1 cgataaccat gcattttaac gcatttgaca taactccgcg cgattatgtc cgtgttactg
       61 atctggaaga caacacctta atcgcagagc tcacgggtag tgttattccg gatgatatca
      121 tttgtaattc aggacaaata ttggtacagc tcaaaacctt tgacaaagtg atggctcagg
      181 gtttttctat ttcctactac aattccagca acctgaacga aatacctcac acggaatttt
      241 cagtctctcc caaccctgcc aaagacctgc tgacactcac attccaaaac ggccagcaag
      301 aggagtatgc gctgaaaatt ttctcggtag aaggcaggct gctttttgaa aaagcattcc
      361 gcctgtcgcc cggccaacaa aatcaagtta tcgatgtatc tatgctccag ccggggtttt
      421 acctgttaca catcgcgtcg ggcaacgtga acagcactaa aaaaatcatt atcgaataat
      481 attatttatg aagaaaatac acatatattt ttccctgata ggtctttttc ttgcaaacat
      541 atcgctgact tttgccgaaa aaattgacgt aaaaacggca caaaccctcg ccactaattt
      601 ttattatgaa aagtgcctgt tatttggtta tgatttaaaa caaccggaag agcttacgct
      661 gagcttcacg aaaacaaaaa acagcgagga aatgtattat attttttctt cacaaaacgg
      721 ctatatcatt gtagctgccg aagatgatgc ataccctgtg ttagcctact cgtttgaagg
      781 gccctacacg ggcaaaaata tttctcccga attcggctat tggatgagta tttatgaaga
      841 tcaaataata catatccgca aaaactcgat gaaagccgat aaaaaaattt ctgacacctg
      901 gaatttttac cttacggatt ttatttgcga tgagcaactg aaaaacggca ccaaatcggt
      961 agcgcccctg ctcatgtcca cctgggatca gggaacctat tacaactact tatgtcctcc
     1021 cgatcctgcc ggcgccggtg gccatgtcta ttccggatgt gtagctaccg ccatggcgca
     1081 ggtaatgtat tattacagat atcctgtgca aggcaccggt tctcatggat attattcaaa
     1141 ttatggttac ctgtcggcca attttggagc aactacctac caatggaatg agatgcaaaa
     1201 caatatcggc ggcaaattca actacagcat ggcgctcata cagttgcact gcggcatagc
     1261 catcgacatg gattacagtc ccgatgggtc aggagccagt atgtacgatg ccgcctctgc
     1321 catgaaatca tatttcggtt attccgcttc cacacaatta tatcgtaaag atgattacac
     1381 cgaaacccag tggaaaaata tgctgattga cgacctggac aacaaaatgc ccatacaata
     1441 tgccggctat ggcgaaagcg ggcatgcctt tgtgtgcgac gggtatcagg gcaccgactt
     1501 ttttcatttc aactggggat ggagcgggta ttacaacggc tatttttacc tgaacaacct
     1561 caacccgggc tacactttca gcaactggca gcaggccatc ctgaactctt accctgccac
     1621 cgcttttcct cagcaatgca gcaacaccac actggttcag agcacctgcg gcaccgtgga
     1681 agacggcagc agccctgcga atgattatga gaacaacaaa gactgtatgt ggctgattgc
     1741 gcctaccgat cccgtagatt acatcaggat tacatttgaa aggttgaaca ccgaagccgg
     1801 caatgacttt atcactattt acgatggaga aaccaccagt gaccctgtgt taggtgtttt
     1861 ttcgggcagc accattccat ctgatgtttt atctaccggc aacaaggtac tggtacgttt
     1921 taccaccaac agttcggtag tggctgatgg ctggctgttg acctacaacg gaaaaatcac
     1981 cagtttttgc agcggagtaa cagaattgac agcaccttcg ggagtgattt ctgacggaag
     2041 cggcacctat aactataata acagtacgct gtgccactgg cgtatcagcc ctccggatgc
     2101 cggtaccatt tccatcacgt tcaacaacct ggatttagcg caggacaatg attttgtcaa
     2161 aatttatgat gacaattccg acaccgaact gatgactttg catgccggag acctgccacc
     2221 gacgtttacc acatacaccg gaaaagtact gttacttttt tttaccagcg ttatggataa
     2281 tgccggcgga tgggaacttt cctacacctc tgccccgctt tcggtgagtg aaaaccccga
     2341 agcttttgtc agcatagccc ccaaccctgc ttcggaatat gtcaaattac aggcctttct
     2401 gccgcaggca acaacagtag accttgccat ttgcaataat ctgggcagtg ttgttttatc
     2461 aaggcaatta tcatcaaccg atggccgctt cgacgaggcc gtggatgtca gtgatctttc
     2521 gcagggaatt tatttcctga aacttgctgc tgactcattt cagcaaacgc agaagctgat
     2581 tatccgttaa caccgtccgg ttttttgtta ataatcgtgt tgatttttca gggtcagcat
     2641 aagcgtgttt taccagttta ttcatacttt tgtatttatg agcaatacat tcggagaact
     2701 gttcaggcta agtatttttg gtgattcaca cggaccttcc atggggggca tcattgatgg
     2761 ctgtccttct ggtcttgtca tcgacgaaaa caaaatcctt gcagacctgc aacgccggca
     2821 aagcggcacc accagtttcg cttcggcacg caaagagccg gacaagttca cgttgctatc
     2881 gggcatcagc caggggaaaa ccacaggagg gcctataggg tttctcattc ccaacaagga
     2941 tagcaaacgg gcagattacc aacatctgga cgatatcttc aggccttcac atgctgattt
     3001 tacttattat gccaaatacg gcaccatgga aagtgcagga aaactgcacg cttcggggag
     3061 aatctttgtt cccgtggtgc tggccggctc tatagcaaag cagatacttg ccctgcatgg
     3121 tatcagggtc tttgcctacg tcaggcagat tggaaccatt tccacccctt tcagctatga
     3181 agaaatcgac ccggaaacaa tagaaaacag tcctgtacgt tgtcctgata agaaagcaac
     3241 aaaagaaatg ctccacctgc ttgataagct aaaaaaactt ggcgacagta ccggcggcat
     3301 catcagtggt gttgtcgagg gttgtcctcc cggactgggc gacccggtgt tcggcaaact
     3361 ccaggcaaaa ctggctcaag ccatgttttc tataaacgcc gtcaaaggtt ttgaatatgg
     3421 cagtggtttt gccgccgcag gcatgaaagg cagcgagcac aacgaccttt ttgtggtgaa
     3481 acaaaaccgc ctgcg
//
LOCUS       DAOSFP010000251         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_24981_length_3492_cov_3.771603, whole genome shotgun sequence.
ACCESSION   DAOSFP010000251 DAOSFP010000000
VERSION     DAOSFP010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24981_length_3492_cov_3.771603"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLI16_07665"
     CDS             complement(<1..866)
                     /locus_tag="PLI16_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84474.1"
                     /translation="MTFFFKYYFSLLSFMMLLNIAVTQGQKNTDYYMGKGQEELQKNN
                     FTGAIALFNKVIEEKKENHLAYFYRGFAKMQLGDLIGAESDFERTTMIHRGYSGGYYY
                     LGIIKAEKKDYFDALKNFDRAIELNCTNTDYFNARGYVKTMIWDTMAAEEDLNQSILL
                     NNANVNAWYNRSVLWLNSKKYEKALADCNKAIGINPAFDELFLLRGHIRLCMEDSLSA
                     YKDFEYVLSRDSGNIAAYYNLALYYHHTKDYQNAMRHYSKVIALNPYNSECYFNRAAL
                     YAENEQYQEAVS"
     gene            complement(1026..3131)
                     /locus_tag="PLI16_07670"
     CDS             complement(1026..3131)
                     /locus_tag="PLI16_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOH84475.1"
                     /translation="MKPPLKILFFLVAALTLSLAGLPAFCQEIEPDSTIDEQVQQQIE
                     NYSEQTEAEIDYVDMLADLEYFLKHPVNLNRADYEELTALKILNEIQINNLIDHIQKN
                     GKLLSVYELQSVQGFDVDLISRLLPYITIGPENERQTFNFKDLLRYGKNDVFIRYQRG
                     LEKSKGYEKSDTTEPHYLGSPDKLYVKYRYNYINKVLFGFTAEKDAGEQFFKGINKYG
                     FDYYSGFVNVRNIGIIKSILVGDFNVQFGQGLTLWTGLSFGKGSEPVNIKKLGRGISP
                     YSSSYESGFMRGTGFTIGFKNFELSAFYSKKNIDASLNGSDTLDNDEFYITSITESGY
                     HYTQSLADKKNTLNQQVYGGHLAYKTRKINIGVTAYGMHLAAELLKEQQPYNMFDFKG
                     KNNFNIGIDYSYIFRNFNLFGETSYSRNKYNPKGGWATINGLMVSLDRYVSLVVAYRH
                     YQRNYQSMFASPFLESGNAYNESGFYTGLSIRPHYRVQINAYADFYSFPWLRYLVNSP
                     SKGFDCNLQLQYKPTKKINIVLRYKYENKQTNSDDEDAAIDYPVPYTRQNYRFQVTYP
                     VSASLNMGNRVEVSRYKNHTADPEYGYMIYQDIRYKSNKFPLAASFRFALFDTKSYDT
                     RMYAYENDVLYAYSIPSFYGKGIRYYLMLKYKLGRHIDIWLRFAQTFYNDRNVIGSGW
                     DEINGNKKSEIKAQVRFKF"
     gene            complement(3137..>3492)
                     /locus_tag="PLI16_07675"
     CDS             complement(3137..>3492)
                     /locus_tag="PLI16_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84476.1"
                     /translation="PIQVKLASYNDERLPLIFKIGLSYTFSDKAVLAVEVEKDNNFKP
                     VFKTGLEYHVAKPVFVRIGVATTPFVYSFGAGFEFYHFKLDISASRHPVLGFTPQASL
                     VYDIHPIISKKNISK"
BASE COUNT          897 a          715 c          800 g         1080 t
ORIGIN      
        1 tctgataccg cctcctggta ctgctcattt tcggcgtaca gggcggcccg gttaaaatag
       61 cattcggaat tgtagggatt gagggcgata accttgctgt agtgccgcat agcattttga
      121 tagtctttcg tgtggtgata atacagggca aggttgtaat aagcggcaat gttgccggag
      181 tcgcgggaaa gcacatattc aaagtcctta taggcggaga gggaatcctc catacacagc
      241 ctgatgtgcc ctctcaacag aaataattcg tcgaaggccg ggtttatccc gatggctttg
      301 ttgcagtcgg ccagggcttt ttcgtacttt ttgctgttaa gccataacac gctcctgttg
      361 taccaggcgt ttacgttggc attgttcagg aggatgcttt ggttaaggtc ttcttccgca
      421 gccatggtat cccatatcat cgtcttgaca tagccgcggg cattgaaata atcggtgttg
      481 gtgcaattta attcaatggc cctgtcaaaa tttttcaggg catcaaaata gtcttttttt
      541 tctgctttga tgatgccaag gtaatagtat cctccactat aaccacggtg tatcatggtg
      601 gttcgttcaa aatcgctttc ggcgccaatc aaatctccca attgcatttt tgcaaaaccc
      661 ctgtaaaagt aggcgagatg gttttctttt ttttcttcga taactttgtt aaacaacgca
      721 atggcgccgg tgaaattatt tttttgcagt tcctcctggc ctttgcccat ataatagtcc
      781 gtattttttt gtccctgcgt tacggctata tttaatagca tcataaatga caacagggaa
      841 aaataatatt tgaagaaaaa tgtcacggag cttatgtaat atttttcgtg tggtaacgac
      901 gatttatttt ttactgacat tattaaccga aaaaaacgaa aaatatttta tcttcattga
      961 catgctatta ctgaccagat gtgtgaaaca cctgtcacgg gcagtgtaac caccgcattg
     1021 gaccattaaa atttaaacct tacctgagct ttgatttcgg attttttgtt gccgttgatt
     1081 tcgtcccagc ctgagcctat cacatttcta tcgttataaa atgtctgggc aaaacgcaac
     1141 cagatatcga tatgccggcc aagtttatac ttcaacataa ggtaataacg tatccctttg
     1201 ccataaaaac tggggatgga atacgcatac aacacatcgt tttcgtaggc gtacatcctg
     1261 gtgtcgtacg atttggtgtc gaacagggca aaacgaaacg aggcggccag cgggaatttg
     1321 ttggatttgt accgtatgtc ctgataaatc atatatccgt attcggggtc tgccgtatga
     1381 tttttatagc gcgatacttc cacacggttg cccatattca gtgatgcaga tacgggataa
     1441 gtcacctgga aacggtagtt ttgacgggta taaggaacgg gataatcaat agcggcgtct
     1501 tcatcatcgc tgttggtttg cttattttca tatttatagc gaagcactat gttaatcttt
     1561 tttgtaggct tgtactgaag ttggaggttg cagtcgaagc ctttggatgg gctgttgacc
     1621 agatacctca gccaggggaa ggagtaaaaa tcagcatagg cgttgatctg caccctgtag
     1681 tggggacgga tgctcaatcc ggtataaaac ccgctttcgt tgtatgcgtt gccgctttcc
     1741 agaaaaggcg aggcaaacat tgattggtag ttgcgctggt aatgacggta ggcaacaaca
     1801 agtgatacat aacggtcgag gcttaccata agtccgttga tagtggccca tccgcctttg
     1861 ggattgtatt tgttacggct gtacgaggtt tcgccaaaca gattgaaatt tctgaaaata
     1921 taactgtaat cgatgccgat attgaaattg ttttttcctt taaaatcaaa catattgtaa
     1981 ggctgctgtt ctttcagcag ttctgccgcc agatgcatac cgtaggcggt aacaccgatg
     2041 ttgatttttc ttgttttata ggccaggtgg ccgccataga cctgttggtt cagagtgttt
     2101 tttttgtcgg ccagcgattg ggtgtagtgg taaccgcttt cggttatcga ggtgatataa
     2161 aactcgtcat tgtccagggt gtcgcttcca ttcagcgagg cgtcgatatt ttttttggag
     2221 taaaaagcgg aaagctcaaa atttttaaaa cctatggtaa atccggtacc acgcataaat
     2281 cctgattcgt aagaggagga ataggggctg atgccgcgtc cgagtttttt gatgttcacg
     2341 ggttcgcttc cttttccaaa actaagccct gtccataaag tgagtccctg cccaaactgc
     2401 acattgaagt cgccgacgag gatactcttg atgatgccaa tattcctgac attgacaaaa
     2461 ccggaataat agtcgaatcc gtatttattt attcctttga aaaactgttc tccggcatct
     2521 ttttcggccg taaatccgaa caagaccttg ttgatatagt tataacggta ttttacataa
     2581 agcttgtcgg gactgccgag gtaatgcggt tcggtagtat ccgatttctc gtatcccttc
     2641 gatttttcaa gcccgcgctg gtagcggatg aatacatcgt ttttcccgta tctcaatagg
     2701 tccttgaagt taaatgtctg cctttcattt tcaggaccta tggtgatgta tggcagcagg
     2761 cggctgataa ggtccacgtc gaagccctga acggattgta attcatatac cgataataac
     2821 ttgccatttt tttgaatgtg atcaatcagg ttgttgattt gaatttcgtt caatattttc
     2881 agggcggtca attcttcata atcggccctg ttgaggttca cgggatgttt cagaaaatac
     2941 tccagatcgg ccagcatgtc cacgtaatct atttcggctt cggtttgttc gctgtagttt
     3001 tcaatctgtt gctgcacctg ttcgtctatg gtgctgtctg gctctatttc ctggcaaaag
     3061 gccggtaggc cggccaatga aagtgttaac gctgcaacaa gaaaaaacaa tattttcaac
     3121 ggtggtttca tcagtactat ttgctaatgt ttttcttcga aattatcggg tgtatgtcgt
     3181 aaaccagcga tgcttgcggt gtaaatccca gcacgggatg gcgcgaagca gaaatgtcaa
     3241 gtttgaaatg ataaaattca aaacccgctc cgaacgaata aacaaaaggt gttgtggcaa
     3301 caccaattct tacaaaaacc ggcttggcaa catggtattc cagtccggtt ttgaacaccg
     3361 gcttaaaatt attgtctttt tctacttcca cagccaaaac tgccttatca gaaaatgtgt
     3421 acgacaagcc gattttaaaa atcaggggca gccgttcgtc gttgtatgag gcaagtttta
     3481 cctgtatggg at
//
LOCUS       DAOSFP010000252         3451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_25421_length_3451_cov_3.683451, whole genome shotgun sequence.
ACCESSION   DAOSFP010000252 DAOSFP010000000
VERSION     DAOSFP010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25421_length_3451_cov_3.683451"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="PLI16_07680"
     CDS             complement(<1..810)
                     /locus_tag="PLI16_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOH84477.1"
                     /translation="MFCYQCQETAKGVGCTIAGVCGKKEDVSNLQDLLIHVLKGISLY
                     SSKARSAGYSDNKLNRFVIESLFTTITNANFDKNVFYERIRKALEWREQIKNVAASKG
                     VDVTKITHDAALWTADTNEAFEEKAKSVGVLSYQDEDIRSLVHLLLFGIKGTAAYAEH
                     ASNLGFENEQVHTFIQHALAEITRNDLEVPYLVNMVMECGKNGVDVMSLLDAANTSRY
                     GNPEITKVDIGVRNNPGILISGHDLRDLEDLLDQTEGSGVDVYTHSEMLPAH"
     gene            complement(852..1715)
                     /locus_tag="PLI16_07685"
     CDS             complement(852..1715)
                     /locus_tag="PLI16_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOH84478.1"
                     /translation="MKRTIIKIDEEKCTGCGICVSGCHEGALQIIDNKARLVSELFCD
                     GLGACIGDCPEGAITLEEREAEPYDEIKVMDRISGQGKATILAHLRHLRDHNEKEYVK
                     QSLQYLKEHNLFPEITEEFVIERKVADHAHHHGGGCPGSKAMDFRDIKVPAQTSAVQI
                     DSQLRQWPVQLHLVNPAASYFKNADVLIAADCVAFAYGNFHNDFLKGKSLIIACPKLD
                     SNTESYVQKIKLMIDESKINTITILMMEVPCCGGLLHLTKMAMQQASRKVPIKTVYFS
                     IQGKVLSEEWV"
     gene            complement(1898..2299)
                     /locus_tag="PLI16_07690"
     CDS             complement(1898..2299)
                     /locus_tag="PLI16_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOH84479.1"
                     /translation="MSHIVNISEAATIGIHVVVLIARSKNNNMNVKKLSELTGASKNH
                     IAKVMQRLVKENFVKSTRGPAGGFVLNTPPEDISLLKIYESIEGELSITKCPFDQQIC
                     PFEECLMGGIVHKVTNELREYFKNKTVKELM"
     gene            complement(2457..3296)
                     /locus_tag="PLI16_07695"
     CDS             complement(2457..3296)
                     /locus_tag="PLI16_07695"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOH84480.1"
                     /translation="MLLVLEYSLQNLVDAGIDGRTGDIQNRRKTCSHIGVFLIGVAVG
                     TFFTGSDFLLNEFNQVFWLNKTRGLEAVFNFQNVTLGLAVFSLSRILGAQYFINSIGD
                     DNIAIRSRRQVGYNTLLFLVFFLTFIFMLLLSDGFSYSPDNGIVSMEPNKYFHNLIEM
                     PAVMLLFITGVVLVLSGIIITWFKKSTKGIWFSGPGTILTVLALLLLAGLNNTCFYPS
                     ASYLQSSLTIQNSSSSQFTLTVMSYVSLFIPFVLFYIIIAWRAINKKRINQEDLSETD
                     HKY"
BASE COUNT          966 a          703 c          696 g         1086 t
ORIGIN      
        1 atgtgctgga agcatttcac tgtgtgtata aacatctact ccgctgcctt cggtttggtc
       61 caggaggtct tccagatcgc gcaggtcatg gccgctgatt aaaattccgg gattgttgcg
      121 tacgcctata tcaaccttgg taatttcggg attgccgtaa cgcgaggtgt tggcagcatc
      181 cagcagcgac atcacatcca ctccgttttt accacattcc atcaccatat ttaccaggta
      241 ggggacttcc aggtcgttgc gtgtaatttc ggccagggca tgttgaataa aggtatgaac
      301 ctgttcgttt tcaaaaccta aattagaagc atgttctgca taggccgccg tgcctttgat
      361 accaaacaac agcaggtgca ccagggagcg gatatcctca tcttggtaag agagtacacc
      421 tactgatttt gctttttctt caaaagcttc gttggtgtcg gcggtccaaa gggctgcatc
      481 gtgagtgatt tttgtaacat caacaccttt tgaagcagca acgtttttta tttgttcgcg
      541 ccattcgagt gccttgcgaa ttctttcgta aaatacattt ttatcgaaat tggcattggt
      601 gatggtggta aacaaacttt cgatgacaaa tctgttgagt ttgttgtcgg aatagcctgc
      661 actgcgagct tttgagctat ataacgaaat gcccttaagc acgtgtataa gcaaatcctg
      721 cagattggaa acatcttctt ttttgccgca aacaccggca atggtacagc caactccctt
      781 ggctgtttcc tgacattgat aacaaaacat actcataatt tttttttagt tttatttgta
      841 aatataatga attaaaccca ttcttcactt aaaactttcc cctgaatgct gaaataaact
      901 gttttgattg gcacttttcg ggaggcttgc tgcatggcca ttttggttag gtgcaataat
      961 cccccgcaac acggaacttc catcatcagg atggtaatgg tattgatttt cgattcatcg
     1021 atcatcaatt ttattttttg cacgtatgat tcagtatttg aatcaagttt tgggcaggca
     1081 ataatcaggc tttttccttt taaaaaatca ttgtgaaaat tgccgtaggc aaaggcgaca
     1141 cagtcggcgg caataagtac atcggcattt ttaaagtagg aggcagccgg attgacaaga
     1201 tgtaattgca cgggccattg cctgagctga gagtctatct gcaccgcgga tgtttgtgcc
     1261 ggtactttga tgtcgcgaaa atccatggct tttgatccgg gacaaccgcc gccatgatga
     1321 tgagcatggt ctgcaacttt tctttctata acaaattctt cggttatttc cgggaataag
     1381 ttgtgttctt taagatattg taaactttgt tttacatatt ctttttcgtt gtggtcgcgc
     1441 agatggcgga ggtgtgccag gatggtggct ttgccctgtc cgctgatacg gtccattact
     1501 ttaatttcat catacggctc agcttcgcgt tcttcgaggg tgatggcccc ttcagggcaa
     1561 tctcctatac acgctcccag cccgtcgcaa aaaagttcgc taaccaaacg ggctttgttg
     1621 tcaataatct gaagggctcc ttcatggcat ccgctcacac atatgccgca accggtgcat
     1681 ttttcttcgt caatttttat gattgtcctt ttcataacta ttttttttcg tatttggttt
     1741 aaaaaggtaa ttaagtacgc aaatgtacaa ataaaaaaaa taacttcaac aaaaattgta
     1801 attataaatt ataaattatt gataataagt aaaatactga tctaatttat aattgcttta
     1861 aacacaatcg taaagaaata ttttacctgt ctaagaatta cattaattct ttgacagttt
     1921 tattcttaaa atattcgcgc aattcgttgg taaccttgtg tacaataccg cccatcaggc
     1981 attcttcaaa tgggcagatt tgctgatcaa acgggcactt tgtaatggat aattcgcctt
     2041 cgatggactc gtaaattttg agcagagaga tgtcttcagg tggtgtattt aatacaaaac
     2101 caccggcagg tccacgggtt gatttaacaa aattttcttt taccagacgt tgcattacct
     2161 tggctatatg gttttttgaa gctccggtaa gttccgataa ttttttcacg ttcatattat
     2221 tgttttttga acgtgctatc agtacaacta catggatgcc aatagtagca gcttctgata
     2281 tgtttacaat gtgcgacatg gtgtgttttt attagggttg ttatacaatg tgtttttttt
     2341 caaaataatt gatggcgatt ctgataagct tataattgat gtaaataaat acaggccaac
     2401 tgatatacca caaaactgat gctaatgctg aatcatacat tgtttgattg ttttaattaa
     2461 tatttatggt ctgtttccga caaatcttcc tgatttattc tttttttatt gatagctctc
     2521 catgcaataa ttatataaaa tagtacaaaa ggaataaaca gcgacacata actcataaca
     2581 gtaagggtaa attggctgga cgagctgttt tgtatggtca gggagctttg caggtaggat
     2641 gccgatgggt aaaaacaggt gttgttgagt ccggcaagca ataatagcgc taaaacagta
     2701 agtattgttc ccgggccgga aaaccagatt cctttggtgc tttttttaaa ccaggtgatt
     2761 ataatacccg acaataccaa cacgacacct gttatgaaca gcaacatcac cgcgggcatt
     2821 tctataagat tatggaaata cttatttggc tccatactga ctataccgtt gtctggcgaa
     2881 taggaaaaac catcacttaa cagcaacata aaaataaatg tcaggaagaa cactaaaaac
     2941 aacagcgtgt tgtaacccac ctgcctgcgg cttctgatgg caatattatc atcacctatg
     3001 ctgttaataa aatattgtgc gcctaatatt cgggaaagcg aaaatactgc cagcccaagg
     3061 gtcacattct gaaaattaaa taccgcctca agtccgcggg ttttgtttaa ccagaacacc
     3121 tggttaaatt cgttcaacag aaaatccgag ccggtaaaaa atgtcccaac agccacaccg
     3181 ataaggaaaa cacctatgtg agaacaagtc tttctccgat tctgtatgtc accagttcga
     3241 ccatcaattc cagcatcaac gaggttctga aggctatatt cgagaaccag taacacatga
     3301 gaattctgat tcgatacgtt atattttttg ccctgttaat tgcatgggga ggaatagcgt
     3361 cagcggctgt tcctcccgat gccgtttcca ttgaaatcgc cgcatccaat gatcatcccg
     3421 cgatcggcga ttcgcttacc attgtttgca c
//
LOCUS       DAOSFP010000253         3443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_25509_length_3443_cov_3.621311, whole genome shotgun sequence.
ACCESSION   DAOSFP010000253 DAOSFP010000000
VERSION     DAOSFP010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25509_length_3443_cov_3.621311"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1277)
                     /locus_tag="PLI16_07700"
     CDS             complement(60..1277)
                     /locus_tag="PLI16_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOH84481.1"
                     /translation="MWEVIKANQRKSMALFVVMTAVLVGLGFIIGYAYDPEGGGAFAG
                     MAIAAIIMIIMSLISIKSGSKIMLAASNAKEVTPEVHPQLFNVVEEMRIAAGIPKMPK
                     VYIINTDAPNAFATGMSPDNAAVAVTAGLLARLNRDELQGVIAHEMSHITNRDIRFMT
                     IAGVMLGSIVIIAEIFLRSLWITGGRSRNSNSGGNQGQIIIMLIAMVFAILAPIMAQL
                     LYFAISRKREYLADASAVRLTRYPEGLASALEKISGNTAEMPMANRVTAPMYIANPLK
                     QKGMKMADLTSTHPPISERIRILRAMSGGAGFVNYQQAYKNVTGKDKTIIPESEINTQ
                     DSAGLREGTGMAAFADPKTTTRSSHDIMMMVDKYTFINCNCGMKIKVPANFGGNTVYC
                     TRCGAKYTVTKNQ"
     gene            complement(1317..1871)
                     /locus_tag="PLI16_07705"
     CDS             complement(1317..1871)
                     /locus_tag="PLI16_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOH84482.1"
                     /translation="MTALWIILGIIAVLAFMVIGMYNSLIKLRNQVKNAWAQIDVQLK
                     RRHDLIPNLIETVRGYMIHERQTLESITNMRSQAMNATNMQDRVKAEGELTNMLGRLQ
                     VAVEAYPDLKANQNFLALQEELTSTENKISYARQSYNDQVLFYNNKKQIFPSNIIAGM
                     FNFKDEVFFEIQDQSEKEVPKVSF"
     gene            complement(1894..2298)
                     /locus_tag="PLI16_07710"
     CDS             complement(1894..2298)
                     /locus_tag="PLI16_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOH84483.1"
                     /translation="MICPVCKKPMLVLELQQIELDHCQECGGIWLDEGELELLLGDAE
                     DKNDLMASFSADLKNPEKKRRCPACSKKMNKVLVGKNNRVLLDKCRKNHGFWFDSGEL
                     NEVIELGSGEKYNKILQLLNEMFAYKLKSNIN"
     gene            complement(2541..>3443)
                     /locus_tag="PLI16_07715"
     CDS             complement(2541..>3443)
                     /locus_tag="PLI16_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84484.1"
                     /translation="AIYDKWEIIAGLPATRYIIIKNDNSLYKLDSTQYGFRSLSRDLA
                     FMTSVQIRVFMLTYNYSITGDTLRMVNQTDSIVMKKNSNGPDETSWVTMVTVTDTLYN
                     PAVGGDGRQDIGFDGANILWAGNWNTSTLYKINPANGTHTTLTLAGNYYSPSTNYASS
                     SIWIVNNYKIDKINPNTGTVLQSSPNLCLHRIKSLAQVGQKMYYSSDGYIYTWDISTD
                     EILQHFPCDAEGMEYAGGYLYLLQDHQIHKCQLSPFKCVLTYYINSPFSGGNIGGLTY
                     DCASFWVVGEDPYNYEYVLAKLSI"
BASE COUNT          907 a          735 c          716 g         1085 t
ORIGIN      
        1 aaattgtgtc atgccggatg ggttttatta attgaagata tttgtaaggt aatgaaaact
       61 tattggtttt ttgtaacagt atattttgct ccgcaacgtg tacagtaaac tgtgtttcct
      121 ccgaaattgg cgggcacttt gattttcatg ccgcagttac agttgataaa tgtgtatttg
      181 tcaaccatca tcatgatgtc gtgtgagctt ctggttgtgg ttttggggtc ggcaaaagcc
      241 gccataccgg tgccttcgcg aagtcccgca ctatcctgtg tgtttatttc cgattcggga
      301 ataatggttt tgtcttttcc ggtaacattt ttataggcct gctggtagtt tacaaaacct
      361 gcaccccccg acatggcgcg taaaatgcgg atacgttccg aaataggagg gtgggtgctg
      421 gtcaggtctg ccattttcat ccctttttgt tttagcgggt tggcaatata cataggtgct
      481 gtaaccctat tggccatagg catttcggct gtattgcccg aaattttttc cagggcagaa
      541 gccagccctt ctggatatcg tgtcagtcgc acagccgaag catcagcgag gtattcacgt
      601 ttgcgcgaaa tggcaaagta cagcaattgc gccataatgg gtgcgaggat agcaaatacc
      661 atagcgataa gcatgatgat aatctggccc tggttgccac cgctgttgct gttgcggctg
      721 cgaccgccgg taatccacag actccgcaaa aatatttcgg caatgataac gatggatccc
      781 agcattaccc cagctatggt cataaaccgg atgtcgcggt tagtaatgtg cgacatctca
      841 tgtgcgataa caccttgcag ctcgtcgcgg ttcagtcgag ccagcaaacc tgccgttacg
      901 gccactgcgg cattgtcggg gctcatgccg gtggcaaagg cattgggggc atcggtattg
      961 ataatataca ctttcggcat tttgggaatc ccggcagcga tacgcatttc ttctaccacg
     1021 ttgaatagtt gcgggtggac ttcaggggta acttctttgg cattgcttgc tgccagcatg
     1081 attttgctgc ccgatttgat ggaaatcaac gacatgataa tcatgataat tgctgcaata
     1141 gccatccctg caaaggcacc gccaccctca ggatcgtaag catagccaat aataaaaccc
     1201 agtcccacca acactgctgt catcactaca aacagcgcca tcgacttccg ctgattagct
     1261 tttattactt cccacataat tggccttaat tagaaataat taaaaattct gtacaattaa
     1321 aaactaactt tgggtacttc tttttccgac tggtcttgta tttcaaagaa tacttcatct
     1381 ttaaagttga acatgccggc gataatattt gacgggaaaa tctgtttctt gttgttgtaa
     1441 aacaacacct ggtcgttgta agactggcgg gcgtatgaaa tcttgttttc cgttgaagtc
     1501 aactcttcct gcaaagccag aaaattttgg ttggctttca ggtcaggata ggcctcaacg
     1561 gctacctgca aacgtcccag catatttgtc agttcacctt cggccttaac cctgtcttgc
     1621 atattggtag cgttcatggc ctggctgcgc atattggtaa tgctttccag cgtctggcgt
     1681 tcatgaatca tgtagcccct caccgtttca ataaggttgg gaataagatc gtggcgccgt
     1741 ttcagttgaa catcaatctg tgcccaggca ttttttacct ggtttcttaa ttttatcagg
     1801 gagttgtaca tccctattac cataaaagca agcaccgcga taattcctaa aataatccat
     1861 aaagcagtca taatttttta cctcctgtaa attttaatta atattagatt ttagtttata
     1921 agcaaacatt tcatttaata gctgtaaaat tttgttgtat ttttctccgc tcccaagttc
     1981 aataacttca ttcagttctc cagagtcaaa ccagaatcca tgatttttcc tgcatttatc
     2041 cagcaatacg cgattgtttt ttcctacaag caccttgttc attttttttg agcaagccgg
     2101 acagcggcgt tttttttccg gatttttaag gtccgcgcta aaagaggcca taaggtcgtt
     2161 tttatcttcg gcatcaccaa gcaaaagctc cagttctcct tcatcgagcc agatgccgcc
     2221 gcattcctgg caatgatcca attcaatctg ctgaagttcc aataccagca taggtttttt
     2281 acatacgggg cagatcatat ttgtttttat aaattgatga attattcaaa aataattaaa
     2341 tgtgaatttt taatatttct atttatcatt attttttaaa atgatttatc aaatttagtg
     2401 atatttttat tttttctgac aattagtcgc aaattccttg tttttgttac attttggcaa
     2461 attattgtca gtgtgatttc cgcttgatgt cggtacacac aggctcaggc agcgatggct
     2521 gatttcatga agactaaagg tcaaatactc agttttgcga gaacatattc ataattgtaa
     2581 gggtcttctc cgacaaccca gaaagatgca cagtcgtatg tcaggccacc aatgttacca
     2641 ccggaaaagg gagaattaat ataataggtt aaaacacatt taaagggact aagctggcat
     2701 ttatgaatct gatgatcctg aagtaaatac aagtatcctc cggcatattc catcccttct
     2761 gcatcacagg gaaaatgctg aaggatttca tcggtcgaga tatcccatgt gtatatgtac
     2821 ccgtcagatg aatagtacat tttttgaccg acctgcgcca gggattttat cctgtgcaaa
     2881 caaagatttg gactgctctg gagcactgtc cctgtattgg gattaatttt gtcgatttta
     2941 taattgttaa caatccaaat ggatgatgaa gcataattgg tactgggcga ataatagtta
     3001 ccggccaggg ttaatgttgt atgtgtgcca ttggcgggat ttattttata aagcgtgcta
     3061 gtattccagt tgccggccca aagtatattc gcaccgtcaa aaccgatatc ttgtcttccg
     3121 tcccctccaa cagcaggatt atacagggtg tccgtaaccg taaccatcgt cacccacgat
     3181 gtttcatcag gtccatttga attttttttc atcacaatac tgtcagtctg attgaccatc
     3241 ctgagggtgt cgcctgtgat agaatagtta tatgttaaca tgaagaccct tatttgtact
     3301 gatgtcataa atgcaaggtc ccttgacaaa gacctgaagc catactgcgt cgaatcaagt
     3361 ttataaaggg aattatcatt ttttattatg atataccgtg ttgctggcag gcctgcaata
     3421 atctcccatt tatcataaat agc
//
LOCUS       DAOSFP010000254         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_25606_length_3434_cov_3.042024, whole genome shotgun sequence.
ACCESSION   DAOSFP010000254 DAOSFP010000000
VERSION     DAOSFP010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25606_length_3434_cov_3.042024"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..2273
                     /locus_tag="PLI16_07720"
     CDS             399..2273
                     /locus_tag="PLI16_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOH84485.1"
                     /translation="MDSLPKKILASFFRQMLFWLLVLELTRLVFVFYNLKFLRDIPVG
                     ETAGVFWYSLPLDVSAICYIMVLPFFFLVIQNFYTTRWLEIAYKTYVFIVLFLLLLIT
                     SVEAGIYSEWKMKLHYKALQYLLHPDEIAASAQSWQILVITLLILLQTLLYYFLYLKI
                     FYYRVSPSKKNYLFSLFFVLLTPGLLFLGMRGGLQQIPINQSQSYFSKHDILNCVSVN
                     SLWNFGHSIADNYSMLDENPFEYYSKEEACAIVSQMFSAPADSSVKVLKNNHPNVVMF
                     ILEGWSADLIESLGGEAGITPFFRKLEKNGILFTNIYTNGTRSQEGMSAIFSGFPAQG
                     ITAVSQQPGKYQKLPGLIQKLKPEGYFSSFYFGGQLIYGNIKSYLVYNGFDQLIEESD
                     FPAPMPRGKLGLHDEFTFPYFLKGLNSQKQPFFSALFTMSTHSPYDYDKPQTIMWPEY
                     EKKYVNAANYADGCLKDFFTEAQKEKWYDNTLFILISDHCHGSYRNHPFYSPDYHKIV
                     MLWCGNVIKDEFMGSRVEKIGSQTDLPALLLSQLEINHDEFNWSKNLWQPSCPEFAFF
                     AYNGGFGLVKRGAQITYETVSNSPSNLIIDTTATITEQELLKQAKSYMQCSFQQYMDF
                     "
     gene            2400..>3434
                     /locus_tag="PLI16_07725"
     CDS             2400..>3434
                     /locus_tag="PLI16_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84486.1"
                     /translation="MKKSYFLLPLLFAFTFFCSLGLYGQNLVLNPSFETVNTANLKCS
                     WYLTNTEFTNAISNWTMPTGGSTDIFHSSLATSCFCSPFSTDASSPGTQAPRTGNSMS
                     AVFVYGSGGCTPYREYLQGELSSPLVPGQQYCIEFYVSLADLSTYACNNLGVYFTTAP
                     FYNSSMCVYSATPQVNYIGIITNKTAWTLISLSFTPTQAFTHFTIGNFYSDAATSTTN
                     VGGTKTQTRYFLDDVDIHLCNTMTVTVNSPTICAGQTATLNATSNLAGTNFTWSTGAT
                     GATINVSPTTTTSYTVTGTASGGVTASAVATVNVNPLPSVSAGALPSAVCNGQCTNLS
                     ANGASSYTWMP"
BASE COUNT          953 a          745 c          682 g         1054 t
ORIGIN      
        1 tgatgagggc aatttttgtt acatttttac tgatttgccc gacagtaaca ccggcagcca
       61 ttgtaaaaac agcggcaaaa agaaaaaacc tgattttttt catgggattt gattgatgta
      121 taaaatgata atgcgaaatg caaagataat gattagatta atcacgtatt aaaacttttt
      181 ttgacttttg gttcaatagg gtgcctctac tttgttgatc gggttgctct aaaaaaatcg
      241 tggcggccga agttttgaaa actacaatat attgaacgta tatgagaatt tgaagaacaa
      301 agacaaccaa aggttttgaa gttattagag gtgcccttta aaaaatattt aatgtgtcat
      361 atgggttcaa tagtgtaaat ttgccgaaaa actaaaacat ggattctctg ccaaaaaaaa
      421 tactggcatc cttttttcgg caaatgttgt tttggctcct tgttttagaa cttacccggt
      481 tggtttttgt tttctataac ctcaaatttc tcagagatat tcccgttggc gaaacagcgg
      541 gcgttttctg gtattcgctg ccccttgatg tgtcagctat ttgctatatc atggtgttgc
      601 cgtttttttt tctggtaatc caaaattttt atacaacacg ctggcttgag attgcctata
      661 aaacatacgt tttcatcgtg ttgtttcttt tgttgcttat cacctcggtg gaagccggca
      721 tatacagcga atggaaaatg aaactgcatt acaaggccct tcaatacctt ttgcatcctg
      781 atgaaatagc ggcctcggca caatcctggc agatactggt gataacactg ctgatcttgc
      841 tgcaaacact gttgtactat tttttgtacc tgaaaatatt ttattatcgt gtttcccctt
      901 caaagaagaa ctatttattt tctttgttct ttgttctgtt gacgccaggc ctgctttttc
      961 tgggtatgcg gggcggcctg caacagattc ccatcaacca gagccagtct tatttcagca
     1021 agcacgatat tctcaactgt gtatctgtca attcactgtg gaacttcggc cacagtattg
     1081 ctgacaacta cagtatgctg gatgaaaatc catttgagta ttatagcaaa gaagaagcct
     1141 gtgccattgt tagccaaatg ttttcagctc ccgcagattc ttctgttaaa gtactgaaaa
     1201 acaaccatcc caatgtcgtt atgtttatcc tggaaggttg gtcggctgac cttatcgaga
     1261 gcctgggggg cgaagccggc atcacgcctt tttttcgcaa gttggaaaaa aatggcattt
     1321 tgtttaccaa catatatacc aatggaaccc gttctcagga aggcatgtcg gctattttca
     1381 gcggattccc cgcacaaggt atcacggctg tttcgcaaca acctggaaaa taccaaaaat
     1441 tgccgggtct gatacaaaag ttgaaacctg agggctattt ctcttctttt tattttggcg
     1501 gacagcttat ttatggaaac atcaaaagtt atttggtgta caacggtttc gatcaactga
     1561 tagaggaaag cgatttcccg gcccctatgc ccaggggaaa gttaggattg cacgacgagt
     1621 ttacgtttcc atatttccta aaaggactga acagccaaaa gcagcctttt ttttccgcac
     1681 tgtttaccat gagcacacat tcgccgtacg actacgacaa accacagaca ataatgtggc
     1741 ctgaatatga aaaaaaatat gtcaatgccg ccaattatgc agacggctgc ttgaaagatt
     1801 ttttcacaga agctcaaaaa gaaaaatggt acgataacac cttattcatc ctgatttccg
     1861 accattgtca tggctcatac cgtaaccatc ctttttattc tcctgactat cataaaattg
     1921 ttatgctgtg gtgtggcaat gtaattaaag atgaatttat gggcagcagg gtagagaaga
     1981 tcggctctca aaccgacctg cctgcccttt tgctgtcgca gcttgaaata aatcatgatg
     2041 agtttaactg gagcaaaaac ttgtggcaac cctcatgccc agaatttgca ttttttgcct
     2101 ataatggagg ttttggactg gttaagcgcg gagcgcaaat cacgtatgaa accgtttcca
     2161 attctccttc aaatttgatt attgatacca cagcaactat cacagaacag gaactgctaa
     2221 aacaagccaa atcatatatg caatgcagct tccagcaata tatggatttc tgattacgag
     2281 gaaggaggca gtggcttaaa aaaatcgacc ggaaatactg tttcttgaat ggaatttgct
     2341 ttttttttaa tatttttgag cagatatttt atgtataatc caacttcaat actgtctaaa
     2401 tgaaaaaaag ctattttctt cttcctttac tttttgcctt tacttttttt tgtagcttag
     2461 gcctttatgg acagaacctg gtgcttaacc cgagttttga aactgttaat acggctaatc
     2521 tgaaatgtag ctggtatctg accaataccg agtttaccaa cgcaatttca aattggacca
     2581 tgcctacagg cgggtcaaca gatatttttc attcatcact tgctacatca tgtttttgta
     2641 gtccgttttc caccgatgcc agttctcccg gaacacaggc tccccgcacc ggaaactcca
     2701 tgtcggcagt ttttgtttat ggcagcggcg gctgcacacc ataccgcgag tacttgcaag
     2761 gagagctatc ttcccctttg gttccggggc aacaatattg cattgagttt tatgtgtctt
     2821 tagctgattt aagcacgtac gcatgcaaca accttggggt ttatttcaca acagcgccat
     2881 tttataactc cagcatgtgt gtttatagtg ctactcctca ggtaaactat atagggatca
     2941 ttacaaacaa aacagcatgg acattgattt cgctgtcttt cacacccaca caggccttta
     3001 cgcattttac cattggcaat ttttattccg atgcagctac ttcaaccaca aacgtgggcg
     3061 gaaccaaaac acaaacccgt tatttccttg atgatgttga tatccacttg tgcaacacaa
     3121 tgaccgttac tgttaacagt ccaacaatct gtgcagggca gacagctaca ttaaatgcca
     3181 cttcgaacct tgccgggaca aactttacct ggagtaccgg cgctaccgga gccaccatta
     3241 atgtgagccc taccacaaca acttcatata ctgtgaccgg tactgcatct ggcggtgtta
     3301 ctgcttcggc agtcgccact gtaaatgtca atcctcttcc ttcggtaagc gccggcgctt
     3361 tgccaagtgc tgtttgcaac ggacaatgca ccaaccttag cgctaacggt gcttctagct
     3421 atacctggat gccc
//
LOCUS       DAOSFP010000255         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_25807_length_3415_cov_4.220833, whole genome shotgun sequence.
ACCESSION   DAOSFP010000255 DAOSFP010000000
VERSION     DAOSFP010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25807_length_3415_cov_4.220833"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="PLI16_07730"
     CDS             complement(<1..673)
                     /locus_tag="PLI16_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84487.1"
                     /translation="MPKIIRKTVISIVLLLCFSSSYGQPVQRFCLPAGSTSHDYIKNT
                     IILKIKPEYASFCKSKHLDLPVINAVFQQLGVNSLEKKFPAIRPPAQKTNLQGQQYAD
                     LSRIYEITYLASENIEQAINRLMATGMIEYAQPHYIMKPLDYVPDDPLIANQYHLGLI
                     GAFGAWDMVKGDSNMVIGILDWGVDITHPDLIGNIKYNYHDPIDGIDNDNDGYTDNYR
                     GWDMGE"
     gene            complement(726..1172)
                     /locus_tag="PLI16_07735"
     CDS             complement(726..1172)
                     /locus_tag="PLI16_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOH84488.1"
                     /translation="MLPLNLPSYEFSITPGASPKIFDFIRKKYVALTPEEWVRQNFLR
                     FLTEEKKYPPSMILLEQTLKVYKTQKRCDAVVYDNKGKPLMILEFKAPEIAVNQKVFD
                     QIARYNIALRLKYLIVSNGLNHFFCIIDPDKKTYAFQDDIPEYPAL"
     gene            1222..2001
                     /locus_tag="PLI16_07740"
     CDS             1222..2001
                     /locus_tag="PLI16_07740"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOH84489.1"
                     /translation="MKTKNEIVQDWLPRYTGTEMDAFGKYILLTNFNHYLELFAEKFN
                     VPINGENKAMPSATANDITIVNFGMGSANAATIMDLLSAVEPKAALFLGKCGGLKKKN
                     KLGDLILPIAAIRGEGTSNDYFPPEVPALPAFNLQKAVSTSIRELGHDYWTGTVYTTN
                     RRVWEHDDEFKKYLRKIRAMAIDMETATLFTVGFANKIPMGALLLVSDQPMISSGIKT
                     QKSDKKVTEMYAENHLKIGVESLNQLINNGLTVKHLYFDYK"
     gene            2007..3014
                     /gene="holA"
                     /locus_tag="PLI16_07745"
     CDS             2007..3014
                     /gene="holA"
                     /locus_tag="PLI16_07745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOH84490.1"
                     /translation="MKSYNEIIKDLERKIYYPVYFLSGEEPYYIDMISDYIEDNLLQP
                     EEKEFNLHVLYGRDTSIQNIVDRCKGFPMMSNYQLVIVKEAQDIKDIENLEKYISNPV
                     DSTILVVGYKYHKLDKRKSFAKLVEKKAVLFESAKIYDNQVAPWIINYVREKGYSIQQ
                     KAAMMMADFLGTDLGKIVNEISKIIINYPRGSEISAEQVEVNIGISKDFNVFELQKAL
                     GSKNIFKANQIIKYFAENEKDNPIVKVIPILYLFFSKVFLYHHLDDKSANNVASVLSI
                     NRMFIDDYSTAARNYPRQKLRNVFGLLREYDLRAKGVNNDGTSTGDSELMKEMIYKIL
                     H"
BASE COUNT         1027 a          667 c          731 g          990 t
ORIGIN      
        1 tttcgcccat atcccagccg cggtaattgt cggtatagcc gtcgttgtca ttatctatcc
       61 cgtcaatggg atcgtggtag ttgtatttaa tgttgccaat taagtcggga tgcgtaatgt
      121 caacacccca atcaaggata ccgataacca tattgctgtc gccttttacc atatcccatg
      181 ctccgaaagc acctatgagg cccaggtggt attggttggc gatgagcgga tcgtcgggta
      241 catagtccag cggcttcatg atgtaatgtg gttgggcata ttctatcatg ccggtggcca
      301 taaggcggtt gatggcctgc tcgatgtttt cggaagcaag gtatgttatc tcataaatcc
      361 gcgacaagtc ggcatattgc tgtccctgca gattggtttt ttgtgccggt ggccggatag
      421 ccggaaattt tttttcaaga ctgttcacgc ccagctgttg aaaaaccgca ttgataacag
      481 gcaaatcaag gtgtttgctt ttgcaaaaag aagcatactc cggttttatt ttcaggatga
      541 tagtgttctt tatataatca tgagaggtgc tccctgccgg cagacaaaac cgctgcaccg
      601 gctggccgta gctactggaa aaacacaaca acagcactat tgaaataact gttttccgaa
      661 ttatctttgg catgtgttta aaaaattttt gtataaaatt acggtaaaaa ttaattgaca
      721 gcaaattata aggccggata ttcgggaata tcgtcctgaa aagcataagt ttttttatcc
      781 ggatcaataa tacagaaaaa atggttcagg ccgttggata caatcagata ctttagacgg
      841 agggcgatgt tgtaccgggc aatctggtca aaaacctttt gattgacggc tatttcgggc
      901 gctttaaatt ccagtatcat cagtggcttt cccttgttgt catatactac ggcatcgcag
      961 cgtttttgag ttttataaac tttcagtgtc tgttccagca aaatcatcga cggcgggtat
     1021 tttttttctt cggtcaggaa ccttaaaaaa ttttgtctta cccattcttc gggtgtaagg
     1081 gccacatatt ttttgcggat aaaatcaaaa attttcgggg aagctcctgg tgttatcgaa
     1141 aactcgtaag agggaagatt aagcggaagc ataaattatt tttgtatttt tgccttaaag
     1201 ttactcataa aatatttgct gatgaaaaca aaaaacgaaa tagtacaaga ctggctaccg
     1261 cgttataccg ggacagagat ggatgctttt gggaaatata tattgctcac caatttcaat
     1321 cattacctcg aactttttgc tgaaaaattt aatgttccca tcaatggcga aaacaaagct
     1381 atgcccagcg ctactgctaa tgatattacc atcgtgaact ttggtatggg tagcgccaat
     1441 gctgcgacca tcatggattt actgagtgcc gtagaaccca aggcggcact gtttctgggc
     1501 aagtgcggcg gcctgaaaaa gaaaaacaaa ctcggcgacc tgattcttcc tattgccgct
     1561 atcaggggtg aaggtacctc caacgactat tttcccccgg aagtaccggc cctgccggct
     1621 tttaatctgc aaaaagccgt ttcaacctcc atccgcgagt tggggcacga ctactggaca
     1681 gggacggttt atacgaccaa ccggcgcgtt tgggaacatg atgacgagtt taaaaaatac
     1741 ctgcgtaaaa ttcgtgcgat ggccattgat atggaaacag ctaccttgtt tacagtgggt
     1801 tttgcaaata aaattcctat gggcgccctg ttgttggttt ccgaccagcc tatgatttca
     1861 tcgggtatca aaacccagaa aagcgacaaa aaagttacgg aaatgtatgc cgaaaaccac
     1921 ctgaaaatcg gcgtggagtc actcaaccag ctcattaaca acggacttac cgtgaaacat
     1981 ctttatttcg attataaata agctccatga aaagctataa cgaaataatt aaagatctcg
     2041 aacgtaaaat ctattatccg gtatattttt tatcgggcga agaaccttat tatattgata
     2101 tgataagcga ttatatcgaa gataatcttc tgcagcccga agaaaaggaa ttcaaccttc
     2161 acgtcttgta tgggcgcgat accagcattc agaatattgt ggacagatgt aaggggtttc
     2221 ctatgatgtc gaactatcag ctggtaattg taaaagaggc acaggatatc aaagatattg
     2281 aaaacctgga aaaatatatc tcaaatcctg tggattccac catattggtg gtcggttaca
     2341 aataccacaa gttagataag cggaaatctt ttgccaaact ggtcgaaaaa aaagcggtct
     2401 tgttcgaatc ggcaaaaatc tatgataacc aggtggcgcc atggataata aattatgtga
     2461 gggaaaaagg gtattccata cagcaaaagg ccgcgatgat gatggcagat tttctcggaa
     2521 ccgacctggg aaaaatcgta aacgaaataa gcaagatcat cattaattac cccaggggtt
     2581 ctgaaatatc cgccgagcag gtagaggtga acataggtat cagcaaggat tttaacgtgt
     2641 ttgaactgca aaaggccctg ggaagcaaaa atatttttaa agccaaccaa atcatcaaat
     2701 attttgctga aaacgaaaag gataacccta ttgtaaaggt aattcccatt ttgtatttgt
     2761 ttttttccaa agtgttttta taccatcatc tcgacgacaa atctgccaac aatgtagcgt
     2821 cggtactgtc tataaaccgt atgtttatcg acgattattc cactgcagcc cgcaattatc
     2881 cacgacagaa actccgtaat gtgtttggtt tgctccgcga atacgacctg cgggccaaag
     2941 gagtgaataa cgatggcaca tctacgggcg acagcgaact gatgaaggag atgatttata
     3001 aaattttaca ttaaaatcgg gcaacagttt tcgggacttt ctaaaaagca ctctgtggac
     3061 ttttagtaca ctgtttactt ctttaatatg tttttaaaca gaggattaaa tgaaagagaa
     3121 acatcgaaaa attgtatttt gagacagcct tctaagaaac ggatttaacc caagttaagc
     3181 aatagaagtg tgcgaacttg cccagacggt tatataatct attgctttag ttgggtttat
     3241 cccgacttag aaaatgtttt ccgacaattt ttctttgtcg ggattacatt ttctttgtcg
     3301 tttacgccta aaaatgttta gctataatac caaataatca cgatattatg aagaaagttt
     3361 ctatagtggt tccctgtctt aggacagatt ttttataatt cttaattgat ttttc
//
LOCUS       DAOSFP010000256         3382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_26161_length_3382_cov_5.763451, whole genome shotgun sequence.
ACCESSION   DAOSFP010000256 DAOSFP010000000
VERSION     DAOSFP010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26161_length_3382_cov_5.763451"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLI16_07750"
     CDS             complement(<1..219)
                     /locus_tag="PLI16_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84491.1"
                     /translation="MILHTQKNISPEKLQQLADKYEAIVLQASDYNILITPSALKKTE
                     EADKDFFTSSFVFDNDIQLASKKYIPEKR"
     gene            complement(216..1223)
                     /locus_tag="PLI16_07755"
     CDS             complement(216..1223)
                     /locus_tag="PLI16_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOH84492.1"
                     /translation="MKFSASIYSNKEKKLPELIEELDACGVNFFHIDCLDDIRVFDDI
                     ALIRTLSKTPIDLHLITPSPEKYFDLIALHQVDYVTFQYENLTDGLRIPQGLKTEFGL
                     AITSETGLAAFEPFAGTCSFILFMTTTPGMSGGVFNKQNFKKIREFRSQYPGKKIHVD
                     GGINAEVSFIMRNMGVYAAVIGSYLFKNSFIGSAMVQLKSDGVSSHYHIQDFMLEYDE
                     LPVIHEENLSFERVLLSIEQYKMGFTNIADTNGKLLGIITNADIRKGLIRNIGNLNHI
                     DVRQMINRNPAFIYEDDTVGDVLAYIKNLDFPVLFLPVVNRQKQITGAIKFNYLIKGE
                     S"
     gene            1314..1643
                     /locus_tag="PLI16_07760"
     CDS             1314..1643
                     /locus_tag="PLI16_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOH84493.1"
                     /translation="MTIKQAQQTVDEWIKTTGVRYFSELTNMAMLTEEVGEVARIIAR
                     TYGEQSFKDSDKEKNLADELADVLFVLICLANQTGTDLTEALQKNLEKKTARDNMRHK
                     NNSKLTS"
     gene            1718..1801
                     /locus_tag="PLI16_07765"
     tRNA            1718..1801
                     /locus_tag="PLI16_07765"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1752..1754,aa:Leu,seq:caa)
     gene            complement(1930..2784)
                     /locus_tag="PLI16_07770"
     CDS             complement(1930..2784)
                     /locus_tag="PLI16_07770"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HOH84494.1"
                     /translation="MSKSITHINLISKMPTFAACKGLFSVKTFNKTCSIGLIALLLLI
                     KVTNAQIVNVESHRMQNDSIKLAGHAGASFSYQQNNQKELLQLSVSAALQLRSKSLKD
                     VFLMLGSYEVSKSNSSTLTNAGFAHLRYTRKFNSYFRWEVFMQYQSNPVLLLEMRLLA
                     GTGPRFKILNKPGLKSSLGSLYMFEHEKTNETVPETHNHHRISAYFTFTWSLPKKIGE
                     FSTITYYQPRIDYFNDFRITNQTALSFGLGKHIAFVVGLRYLYDAFPPEGVIRNSFSS
                     EMGIKASF"
BASE COUNT          934 a          690 c          729 g         1029 t
ORIGIN      
        1 ccttttttcc ggaatatatt ttttactggc caactgtatg tcgttgtcaa atacaaaaga
       61 tgaagtgaaa aaatccttgt cggcctcttc ggtttttttc aaagccgaag gggtgatcag
      121 gatgttataa tcactggcct gcaacacgat ggcttcgtat ttatctgcca gttgctgaag
      181 tttctcgggg ctaatatttt tttgggtatg gagtatcatg attctccttt gattaaataa
      241 ttaaacttta tggctccggt aatttgtttt tgtctgttaa ccaccggtag gaacaatacg
      301 gggaaatcca gattttttat ataggcaagc acatcgccca cggtatcgtc ttcgtaaata
      361 aaagcagggt tacggttgat catttgccgt acatcaatgt ggttcaggtt gccgatgttt
      421 ctgatgaggc ctttgcggat atcggcgttg gttataattc ccaggagttt tccgttggtg
      481 tcggcgatgt tggtaaatcc cattttatat tgttcgatgg acagcaacac tctttcaaaa
      541 gagaggtttt cctcatgaat caccggtaac tcgtcgtatt caagcataaa atcctgtatg
      601 tggtaatgtg agctcacccc gtcgcttttc agttgaacca tggctgagcc gataaacgag
      661 tttttgaaca ggtatgaacc gatgactgcc gcataaacac ccatgttgcg catgataaac
      721 gacacttccg cgttgatgcc tccatccacg tgaatttttt taccgggata ttgacttctg
      781 aattccctga tttttttgaa attctgctta ttaaacacac caccgctcat gcccggcgtg
      841 gtggtcataa ataaaataaa actgcaggtg ccggcaaacg gctcaaaagc agcaaggcct
      901 gtttcggaag ttattgccag cccgaattcg gttttcagcc cttgtggaat gcgcaaacca
      961 tcagtaagat tttcatattg aaaagtaaca tagtcaacct ggtgcagggc aatgaggtcg
     1021 aagtactttt ccggcgaagg cgtgatgagg tggaggtcta tcggggtttt gctcagcgtg
     1081 cgtatcaggg cgatatcgtc aaaaacccgg atatcgtcaa ggcaatcaat atgaaaaaaa
     1141 ttaacaccgc aggcgtccag ttcttctatc agttcaggca gttttttttc tttgttggaa
     1201 tatatagaag ccgaaaattt catcgcagat ggtaattttt tagcaaaact acaaactttt
     1261 ggctacaata tgctaatttt accttgatgt atttattata aaatgatttg tttatgacca
     1321 taaaacaagc acaacaaacg gttgatgagt ggataaaaac caccggtgtc cgttatttca
     1381 gcgaattgac caatatggcg atgctgaccg aagaagtcgg cgaggtggcc cgcatcattg
     1441 cccgcaccta tggagaacaa tcatttaaag actcggacaa ggaaaaaaat cttgccgatg
     1501 agcttgccga tgtgttgttt gtgcttattt gtctggccaa tcaaaccggc accgacctta
     1561 cagaagcgct gcaaaaaaac ctggaaaaaa agactgcacg cgacaacatg agacataaaa
     1621 acaacagcaa attgacatct tgaaaatttt tgatttggct ataacaaaac tattttctac
     1681 ttttgtcctc cttttaaaaa agttctttac tttttatgcc cgagtggcgg aataggtaga
     1741 cgcgttggtc tcaaaaacca atgagagcaa tctcgtgccg gttcgattcc ggcctcgggt
     1801 acacacaaaa attttaacca actgtaaatt agtgttttta tggttggttt ttttgttgtg
     1861 gtacaaactg ggggacgaat gaagctgaat gccgcgggaa attttttacc agaatctgcc
     1921 gcaattagtt tagaaactcg ccttaatccc catctcgctg ctaaaagaat tccgaattac
     1981 tccttccgga ggaaaagcat catacagata acgcaggccc actacaaaag caatatgttt
     2041 tcccaaccca aaactcagcg ccgtctggtt ggttatcctg aagtcgttaa aataatctat
     2101 ccgtggctga taataagtta tagtactaaa ttccccgatt tttttgggca acgaccaggt
     2161 aaaggtaaaa taagccgaaa ttctgtgatg gttgtgcgtt tcaggtaccg tttcgtttgt
     2221 cttttcgtgc tcaaacatat aaagcgagcc taaactcgat ttcagtcccg gcttatttaa
     2281 aatcttgaac cgcggtccgg tgccggcaag cagccgcatt tcaagcagta atacggggtt
     2341 gctctggtat tgcataaaaa cttcccacct gaaatagctg ttaaacttcc gggtgtacct
     2401 caggtgggca aagccggcat tagtgagggt gctgctgttc gatttggaaa cttcatagct
     2461 gcccagcatc agaaaaacat ccttaaggct ttttgaccgt agctgtaaag ctgccgaaac
     2521 gctcaactgc aacagctctt tttggttgtt ttgctgatac gaaaacgaag cgcctgcgtg
     2581 accggccagt tttatgctgt cgttttgcat gcgatgggat tccacgttga cgatttgggc
     2641 atttgtcacc ttgattaaaa gcaaaagtgc tatcaatcca atactgcatg ttttattaaa
     2701 tgttttcacg ctgaataagc ctttgcaggc ggcaaaagta ggcattttac tgataaggtt
     2761 tatatgggtg atagatttgg acaatatctt ttttgaggtg cctttatgaa actatttatt
     2821 tgagttttgc atgattttta taagtcacac agagccggac aaagcgcggc aatgagggtt
     2881 gagtccgtgt cgaagtgtga caacaacggt cgtcttccct gcctgcgtgc cgaacacttc
     2941 ggcaggcagg gacaatcttc tccttccact tttggtttca ggagaatctg ctaccaatga
     3001 caacaatatt gtatttggct gttttacagt tatttcggtc aggcagagcc gttgttctgg
     3061 ataaaaatga atttgttgaa aaaatttatt gaacaacgag tcgaagtcga ccgaaataaa
     3121 attcgatgaa attcaccaac aatcaggccc catttcgaca cgtcccgaaa gtccctatac
     3181 ctttggccac aaagatttat attatcggga cggctcaacg tgacctgaat ttttgtcatt
     3241 aaatgttatt tttgtgcgca gcataattac cactctgact gaccgttgtt ttgtcctttt
     3301 tgactatatg taaaaatgtt ctttttcata gagttattat cttggcaatc cacatttgat
     3361 tattatttta caattgtatt tt
//
LOCUS       DAOSFP010000257         3363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_26376_length_3363_cov_3.253628, whole genome shotgun sequence.
ACCESSION   DAOSFP010000257 DAOSFP010000000
VERSION     DAOSFP010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26376_length_3363_cov_3.253628"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="PLI16_07775"
     CDS             <1..1002
                     /locus_tag="PLI16_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84495.1"
                     /translation="LMFALPLGIFIIGLFDKAKKVISFSILAGFTAAVLLANQSLLLK
                     LFSGSMFFVGDPKGFFPENLAKFDVFPFKSLFFYEGALQVVFNMTPILEPIYLFVAGL
                     FSLIIVFEILRYILRKIDAVKLTKDYLTLGVTVLFVNVLFLIYMSLSHKGQSWEIPPW
                     TPVEETRYYGQSMLFIEIVLLTMLLYEQYKSKILKALYVSLLAVVLLYAGSYWINENY
                     TVYVKNNRQNTYAGMYQEELKISQIVDGHLDEGHFNVFASPNALTDNFVFDFSGVQAI
                     NCNYTALLNDTVIITHRPVTLFLATPQDSCNLGKLEKFVPEKIAGHNTFDIYKIKIN"
     gene            1005..2198
                     /locus_tag="PLI16_07780"
     CDS             1005..2198
                     /locus_tag="PLI16_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH84496.1"
                     /translation="MELTILMPCLNESETLAICIQKARSFLQRNGIDGEVLIADNGST
                     DGSKEIAVSEGARVVDVSDKGYGNALIGGINAAKGKYIIMGDADDSYDFVNMEPFVEK
                     LREGYDLVMGNRFKGGIAEGAMPFLHRYLGNPVLSFIGRLFFNIKTGDFHCGLRGFNA
                     DRIKMLKLHTPGMEFASEMVVKSSLFKYKMAEVPTTLKKDGRSHPPHLHTWRDGWRHL
                     VFLLMYSPKWLFLIPGLIILILSIIGFGVLSVKMLYLKNIGIDIHTLTYLGFAIILSY
                     QIILFALLSKFYAVNQGLIPVRHQFMKVFKFFTLEKGLVTGLILFFAGLVFSIILFKY
                     WADKDFGNITDFSHTFRLLIPSVILMALGIQTIFSSFILRIIGIIQHVVFLDDEKIQN
                     NTTKK"
     gene            2272..>3363
                     /locus_tag="PLI16_07785"
     CDS             2272..>3363
                     /locus_tag="PLI16_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84497.1"
                     /translation="MKIKFLIIAFLIIIVTTVSFLFSPRPGPEIYKPENYVSLSAHAG
                     FYVNCDADLYINLSQSPKLLLKDKEPRQSRPLYVIAGWLIGTPLQFILEKSGLSGDFQ
                     RNLRGYYLGYVLLNFIILLISFILFKKICDHYAGSNVNNLVLYALMIFLASNSVTKSY
                     FWTAHQQMFVFLTPLLCLLLIIKLPQKNLSLLRLSGISFLLGTLTLAYGSFLLCLPCL
                     LYATFLVNKKYKSLNDFLKMALIFTVFVAPTLLWILILGLNGVKYYNHEADQYRQFVW
                     IIDSLKVSPLFFLKTALGNLYLFILTFRGLVFFLVLFLALFFARRLMSARKKNEDSKK
                     QVVGILFAMAMVFMFFLCMGYYQIRLTFTL"
BASE COUNT          892 a          747 c          698 g         1026 t
ORIGIN      
        1 ctgatgtttg ctttgccttt aggcatattc attatcgggt tatttgataa ggccaaaaaa
       61 gtgatctcat tttcgatact ggccggattc acggcagccg tgctgctggc caaccaaagc
      121 ctgctgctga aactgttctc gggcagcatg ttttttgtag gcgaccccaa aggttttttt
      181 cccgaaaact tagccaagtt tgatgttttt ccttttaaat ctttgttttt ttatgagggc
      241 gccttacagg ttgttttcaa tatgacgccc atcctggagc ctatttattt atttgtcgct
      301 ggattattct ctcttattat cgtgtttgaa attttgagat atattcttcg aaaaattgat
      361 gcggtaaaac tgacaaaaga ttatctgacc ctgggtgtca cggttttatt tgtcaatgta
      421 ttatttttga tttacatgtc gctttcgcat aaagggcagt catgggaaat tccgccttgg
      481 acaccggtgg aagaaaccag gtattacgga cagtcgatgt tgtttatcga aattgtgtta
      541 ctcaccatgc tgttatacga acaatacaaa tcaaaaatcc tgaaagcgct gtatgtttcg
      601 ctgctggccg tagtgttgtt gtatgccggc tcatactgga taaatgagaa ttatacagta
      661 tatgtaaaaa acaaccggca gaacacctat gccggcatgt accaggaaga actgaaaatt
      721 agccagatcg tggacggtca tctcgacgaa ggacacttta atgtgtttgc ttctcctaat
      781 gctttaacag acaattttgt gtttgacttt tcgggcgtgc aggccataaa ctgcaattat
      841 accgccctgc tgaacgacac ggtcattatt acccaccgcc ccgtcacctt gtttttggcc
      901 acgccccagg actcctgcaa tctcgggaaa ctggaaaaat ttgtccccga aaaaattgcc
      961 gggcataata ccttcgatat ttataaaata aaaatcaact agctatggaa ctgacaattt
     1021 taatgccctg cctcaacgaa tccgaaacgc tggcgatttg catacaaaaa gccaggtcgt
     1081 tcttgcaaag gaacggcata gacggagaag tcctcattgc cgacaacggc agcaccgacg
     1141 ggtcgaaaga gattgccgtc agtgaaggag cccgtgtagt tgacgtgagc gacaagggct
     1201 atggcaacgc cctgataggt ggcatcaatg cggccaaagg caagtatata attatgggcg
     1261 atgccgatga tagttacgac tttgtaaaca tggaaccctt cgtcgaaaaa ctccgcgaag
     1321 gatacgacct ggtgatgggc aaccgcttca aaggtggaat agccgaaggc gccatgcctt
     1381 ttttgcatcg ctacctgggt aacccggtgc tatcgtttat aggccggctg ttttttaata
     1441 tcaaaaccgg cgacttccat tgcggactca gaggtttcaa tgccgacaga ataaaaatgc
     1501 tgaaactcca tacaccaggg atggaatttg cttccgagat ggttgtgaaa agttcattat
     1561 tcaaatacaa aatggccgaa gtacctacca ccctaaaaaa agacgggcgt tcccaccccc
     1621 cgcacctgca cacctggcgc gacggctggc ggcacctggt ttttctgctg atgtacagcc
     1681 ccaaatggct tttcctcatc cccggattaa tcattctgat actcagcatc atcggtttcg
     1741 gagtgttatc cgtaaaaatg ctttacctga aaaatatcgg catcgacata cacacgctta
     1801 cctacctggg atttgccatt atcctcagct accagataat tctttttgcg ttgttgagca
     1861 agttttatgc tgtaaaccag ggactgatac ccgtcagaca ccaattcatg aaagtgttta
     1921 agttttttac cctcgaaaaa gggctggtga ccgggctgat actgtttttt gccggattgg
     1981 tattttccat tattctcttt aaatactggg ccgacaagga tttcggaaac atcaccgact
     2041 tttcacacac attccggctg ctgataccct cagtaattct tatggcctta ggcatacaga
     2101 ccattttttc cagtttcatc ctgcgtatca tcggcatcat acaacatgtg gtttttttgg
     2161 atgacgaaaa aattcaaaac aacaccacaa aaaaatgata cagcggctaa aggcgccttt
     2221 taaaacggct gataataata ttttacactg ttttaactga actctgtggc tatgaaaata
     2281 aagtttttga ttattgcatt tttaatcatc atcgttacaa cagtatcttt tttgttttca
     2341 cccagaccag gccctgagat ctacaagccg gaaaattatg tcagcctctc agcacatgcc
     2401 ggattttatg ttaattgcga tgccgacctg tatatcaatc tttcgcagtc gccgaaatta
     2461 ttattaaaag ataaagaacc caggcagtcg cgtccgcttt atgttattgc gggatggctg
     2521 ataggcacac ccttgcagtt tattcttgaa aaatcaggac tttcgggcga ttttcaacga
     2581 aacctcaggg gatattatct ggggtatgtg ctactgaact ttattatcct gttaatcagt
     2641 tttattttat ttaaaaaaat ctgcgatcat tatgccggca gcaatgttaa taaccttgtc
     2701 ctgtatgcct tgatgatttt cctcgcttcc aattcagtaa ccaaatccta tttctggacc
     2761 gcccatcagc aaatgtttgt cttcctgact ccgttgctgt gtcttttgct aatcataaaa
     2821 ctgcctcaaa aaaacctttc gttattacga ctttccggca tcagtttttt attagggaca
     2881 ttaaccctgg cctatgggag ctttctgcta tgcttaccct gcctgttata tgccactttt
     2941 ctggtgaata aaaaatacaa aagtctaaat gattttttga aaatggcgct gattttcaca
     3001 gtatttgtgg cacctactct actgtggata ctgatattgg gtctcaacgg ggtgaaatac
     3061 tacaatcacg aagcagacca gtaccggcag tttgtttgga ttatcgactc actgaaagtg
     3121 tctcctttat ttttcctgaa aaccgcgctg ggcaacctgt atctttttat ccttaccttc
     3181 agaggcctgg tatttttcct ggtgcttttc ctggcacttt tttttgcccg ccggttgatg
     3241 tccgcacgta aaaaaaacga agacagcaag aagcaggtag ttggaatttt gtttgccatg
     3301 gccatggttt ttatgttctt cctgtgtatg ggttattacc aaatccggtt gacatttaca
     3361 ctg
//
LOCUS       DAOSFP010000258         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_26389_length_3362_cov_3.735107, whole genome shotgun sequence.
ACCESSION   DAOSFP010000258 DAOSFP010000000
VERSION     DAOSFP010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26389_length_3362_cov_3.735107"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..3028)
                     /locus_tag="PLI16_07790"
     CDS             complement(170..3028)
                     /locus_tag="PLI16_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84498.1"
                     /translation="MKSKKLLFGIGLLSIVAAFVVVTVVNSNAERKYNPKKSVFYQEF
                     AGAYKWLASIRNNQVTGEISMADIMKVRKEIAELSHTKSIGLQWEDAGPDNVGGRTRG
                     ILIDKDNSNLLFAGGVAGGLFKSTTGGTSWVPVPGMPEVNICCIAQSPITGYYYIGTG
                     ESFANVDGISGTPGFVGSGMYESTDGGETWHQYPNAFPTAQNSIGAEWAFVNDIAIDP
                     VNGRIYAATNKGLKYCDLGSTTWVNPLYLTPTLLNAANANCVEVASDRSVVVAIGNKV
                     WISQGGTGNGEPHTFTEISPVAGAGRTEISIAPSDPNIIYTCIATSGGALKGIYRTTD
                     KGENWTLIGPGGSTSFFLFGNDASNPTYGQGGYDNVIAVNPYNANQVFVGGIRVWEWH
                     LGSQFTQITLGLEEYDAHVDMHAIVFDKRNPGTFYLGSDGGVAKTTNNGQTFQTINKN
                     YNVTQFYSVAMDGGTGIMSGTQDNSSPYVSGTGLYPKKARVLYGGDGGWSAFSFINPD
                     VFFGTMQYAGIWRSPDRGVTYQEATNSEFMSGTMIGSTTPGQDGAGPFITPMLHWESF
                     NNTFSADQTWFIDTLDHVVGDHITVKSQNNRYPFDYVITPADGNLSAGDTLWVQDIVS
                     SHFFVGTATGVWYTRGALDFGKTPQWYNIANIANPHTFAISKDGNYLFVGTISGAVYR
                     ISNILAISDSLSGWYNSPYCVVERALINNFGQAVTSIAIDPNNPQRLLVTLGNYGNTN
                     YVYFCKNALDVAPVFESKQGTTNGKKLPAMPVYSAIFEMNNPNMVIIGTEYGMFATQD
                     ITKTAALIEWTEENDGMAKVPVFAIRQQVYNYPGVTNYGAIYIGTHGKGFFKNTQYLG
                     INDNQNAKENVSGTLSIYPNPAIDNVNISYSLPVKSAVTIKIYDLNGKLVKLANLGAK
                     ASGNQIENIDCSNFGKGTYIVQLLAGNSSRTAKFIVTK"
     gene            complement(3230..>3362)
                     /locus_tag="PLI16_07795"
     CDS             complement(3230..>3362)
                     /locus_tag="PLI16_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOH84499.1"
                     /translation="KYYSPAEFEQLKKIALEKGFEFAESGPLVRSSYHASKHIKTDN"
BASE COUNT          868 a          718 c          754 g         1022 t
ORIGIN      
        1 catgttttgt gctagtttgg taaatggctg ccgccgtaaa aacacttatt atacagagga
       61 tggtggcaat ggtaaaaaat ccctttttca ttgttaatta ttttttattt gtaaatgtgt
      121 ttgttttagc aaaaagccct ggaccttacg atccaaggct ttcaatactt tattttgtaa
      181 cgataaattt agcggttctg ctgctgtttc cggccaggag ctgaacaata taagttcctt
      241 ttccgaaatt gctgcaatca atgttttcta tttgatttcc gctggctttg gcgcccaggt
      301 tagcgagttt aaccagttta ccgttcaggt catagatttt tatggttacc gccgatttga
      361 caggcagtga ataagaaata ttcacattgt caatggcagg attgggataa atactcaggg
      421 ttcctgatac attctctttg gcgttttgat tgtcgttgat gccaaggtat tgggtgtttt
      481 tgaaaaagcc tttcccatgg gtcccaatgt atattgcacc atagttagta acaccggggt
      541 agttataaac ttgctgcctg atggcgaata ccggaacttt tgccatgccg tcattctctt
      601 cagtccattc aataagggcc gcagttttgg taatatcctg ggtggcaaac attccatatt
      661 ctgttccaat aatcaccatg ttggggttat tcatttcgaa tattgcactg taaaccggca
      721 tggcaggtaa ttttttaccg ttggttgtgc cttgtttgga ttcaaaaaca ggagcaacat
      781 ccagtgcatt tttacagaaa tatacatagt tggtgtttcc gtaattaccc aaggtaacaa
      841 gcaacctctg ggggttgttt ggatctatgg caatgctggt aactgcctgg ccgaaattgt
      901 tgatcagggc gcgttctacc acgcagtagg ggctgttgta ccatccgctg agcgaatcgg
      961 aaatagctaa gatattggaa atacgataga ctgctccgga aattgtaccc acaaacaggt
     1021 agtttccatc tttagatatc gcgaaagtat gagggttggc aatattggcg atattgtacc
     1081 attgcggggt ttttccaaaa tccagggctc cgcgcgtgta ccatacgcct gttgccgtac
     1141 caacaaagaa gtgtgaactg acaatatcct gcacccatag ggtgtctccg gcgcttaaat
     1201 tgccgtcagc aggagtaata acatagtcga aaggataccg gttgttctga cttttcacgg
     1261 tgatatgatc tcctaccacg tgatctaaag tatcgataaa ccaggtttgg tcagcgctga
     1321 atgtattatt aaaactttcc caatggagca tgggggtgat aaagggtcct gcaccatcct
     1381 ggccgggagt ggttgagcct atcatggtgc cgctcataaa ttcactgttg gtggcttcct
     1441 gatatgtaac accgcggtcg ggtgaacgcc agatgccggc atattgcata gtgccgaaaa
     1501 atacatcggg attgataaaa gaaaatgctg accatccgcc gtcgccacca taaagtaccc
     1561 tggctttttt gggatacagg ccggtgccac tgacgtaagg actgctgtta tcctgtgttc
     1621 ccgacataat acctgtgcca ccatccatag caacagaata aaactgggtt acattgtaat
     1681 ttttatttat tgtctggaac gtttggccgt tattggtggt ttttgccact ccgccgtcgg
     1741 aacctaaata aaaggtaccc ggatttcttt tgtcaaatac aatagcgtgc atatccacat
     1801 gggcgtcata ttcttccaat cctaaagtaa tttgcgtaaa ctgactgcca aggtgccatt
     1861 cccaaaccct gatgccaccc acaaaaacct ggttggcatt gtaaggattc acagcaataa
     1921 cattatcata acctccctgc ccgtaggtgg ggtttgatgc gtcgttgcca aataaaaaga
     1981 aagaggtgga gcctcccggt ccgatcaatg tccagttttc gcccttgtcg gtggtgcggt
     2041 aaattccttt taacgcacca cctgatgttg ctatacaggt gtagataata ttcggatcgg
     2101 atggagcaat ggaaatttct gttcttccgg cgcctgccac agggcttatt tcagtgaagg
     2161 tatgaggttc gccgtttcct gtaccgccct gtgaaatcca taccttgttg ccgatggcaa
     2221 ccacaacgga ccggtcgctg gctacttcaa cacaattggc gttggcagca ttcagcaggg
     2281 tcggtgtaag atagagggga ttaacccagg tggttgatcc caggtcgcaa tattttaatc
     2341 ctttgtttgt agcggcatag atcctgccgt ttaccggatc aatagcgata tcgtttacaa
     2401 aagcccattc ggcgccgata ctattttgag cagtgggaaa agcattggga tactgatgcc
     2461 atgtttcgcc gccatcagtt gattcgtaca ttccgctgcc gacaaatccg ggggtgccgc
     2521 tgatgccgtc aacattggca aacgattcgc cggtaccgat gtaataatat ccggttatag
     2581 ggctttgtgc aatgcaacaa atgtttactt caggcatgcc gggtacaggc acccatgaag
     2641 tcccgccggt ggtggattta aacaatccgc cggctacacc gcctgcaaat agcaggttgc
     2701 tgttgtcttt gtcaatcaaa ataccacggg tacggccgcc aacattgtcg ggaccagcat
     2761 cttcccactg aaggcctatg gattttgtat ggcttaattc agcaatttct ttacggactt
     2821 tcataatgtc ggccatgctg atctcgccag taacctggtt gtttctgatc gaggcaagcc
     2881 atttgtacgc gccggcaaat tcctgataaa agactgattt ctttggatta tactttcttt
     2941 cagcattgct gttgaccact gttacaacaa caaaagctgc cactatactt aacaatccga
     3001 ttccaaatag tagttttttg cttttcatga gtctatcttg tttattatta attaattata
     3061 attatttatt atttgaattt tcaaaaaacg gtgcaagata ctgcttttta ttataccgac
     3121 aatgaagttt tttccaaaaa aatcatacat aaatttaata ttatgattat caaaattaag
     3181 gataaatatt gaaattttca acatcaaatc agaatccgga tgttttttgt cagttatcgg
     3241 ttttgatatg cttgcttgca tgatatgatg agcggaccag cgggccggat tctgcaaatt
     3301 cgaacccttt ttccagtgct atttttttaa gctgttcaaa ttcggcaggg gaatagtatt
     3361 tt
//
LOCUS       DAOSFP010000259         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_26819_length_3325_cov_3.686544, whole genome shotgun sequence.
ACCESSION   DAOSFP010000259 DAOSFP010000000
VERSION     DAOSFP010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26819_length_3325_cov_3.686544"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLI16_07800"
     CDS             complement(<1..372)
                     /locus_tag="PLI16_07800"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOH84500.1"
                     /translation="MFFLYSVSVFVYTALIKIASLFNSKARRWTQGRKHWHDNLKQAV
                     KNDHPWIWFHASSLGEFEQGRPVMEAFRQKYPQYKILLTFFSPSGYEIRKNYDGADYV
                     CYLPADTPGNVRKFLVTVNPVM"
     gene            complement(384..1136)
                     /locus_tag="PLI16_07805"
     CDS             complement(384..1136)
                     /locus_tag="PLI16_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84501.1"
                     /translation="MKKLLFFLVFLFLCSQARPQVSVNDSCIFSTMLYATYAYQLPGG
                     DLASRFGGNSAIGGGFQIKTKKNWLFGVEGNYLFGNVVKDQFGILDNIATSDSFLITA
                     DGQYADVFFSEQGFAVSLKAGKVIPVFKSNPNSGILIKLQPGFVQHKIKITNPNNTVP
                     PLRGDYKKGYDELANGFSITEFLGYMYMGNKRLVSFYAGFEFTQAFTKCRRTYNFNTR
                     TRDTEQKYDFFYAFRVGWLIPLYKRAPKGYYF"
     gene            1326..2456
                     /locus_tag="PLI16_07810"
     CDS             1326..2456
                     /locus_tag="PLI16_07810"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOH84502.1"
                     /translation="MHKITMVDLKRQYEDIKHEIDTAIHEVIDSTAFINGPAVNRFQK
                     NLEAYLGVKHVIPCANGTDALQVSMMALGLKPGDEVITTSFTFIATAEVIALLGLTPV
                     LVDVDPDTFNIDPVAIERAITPKTKAIVPVHLFGQSADMEAITAIAEKYQLHIIEDNC
                     QAIGADYIFADGHRKKTGGIGIVGCTSFFPSKNLGCYGDGGAVFTNDDNLATKLRQIV
                     NHGMTVRYYHDMIGVNSRLDSIQAAILDIKLKHLDEYAQRRNQAASYYDQAFAAEPRL
                     KTPVRFKKSTHVFHQYTLVTQDTDRGKLQDFLNTKNVPAMIYYPVPLHMQKAYLDPRY
                     RQGDFPVTEMLCKSVISLPMHTHLDNEQLEYITASVLEFFKQ"
     gene            2461..3039
                     /locus_tag="PLI16_07815"
     CDS             2461..3039
                     /locus_tag="PLI16_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOH84503.1"
                     /translation="MEKDFFAHETAIIDQGCTIGKGSKIWHFSHMMTGCELGENCNIG
                     QNVVVSPGVKLGRNVKVQNNVSIYTGVICEDDVFLGPSMVFTNIINPRSAVVRKDQYV
                     KTVVKKGASIGANATIVCGHDIGEYAFIGAGAVVTKEVPAFALVVGNPAKQIGWISEY
                     GHRLNFNEEGIAVCPESNEKYKFHNGEVSKLA"
     gene            3041..3307
                     /locus_tag="PLI16_07820"
     CDS             3041..3307
                     /locus_tag="PLI16_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84504.1"
                     /translation="MKKFFLLIACCCMSIFFTSCEESNCDNCTIQKREFCLMMFEVNC
                     NSAALTTNMDYLVQACGQDEASQWISAVTDSCTQGILLCPAECQ"
BASE COUNT         1025 a          768 c          679 g          853 t
ORIGIN      
        1 cattaccgga ttgaccgtta ctaaaaattt gcgcacattg ccgggggtat cggcaggaag
       61 ataacaaaca taatcagcgc catcgtaatt cttcctgatt tcatatcccg aaggtgaaaa
      121 aaaagtaagt aaaattttgt actgcggata cttttgcctg aacgcctcca tcaccgggcg
      181 cccctgctcg aactcgccaa gcgaagaagc atgaaaccag atccagggat gatcattctt
      241 tacagcctgt ttcaggttgt cgtgccagtg tttccgccct tgtgtccacc ggcgggcttt
      301 agaattaaac agggaggcaa tttttatcaa tgccgtgtaa acaaatactg atacggaata
      361 taaaaaaaac atcagcagaa tttttagaaa taataaccct tgggggccct tttatacaaa
      421 gggatcagcc atccaacacg aaaagcataa aagaaatcat atttttgctc tgtatcgcgt
      481 gttctggtgt taaaattata tgtcctgcgg cattttgtga acgcctgggt aaattcaaac
      541 cctgcataga atgacaccag ccgcttgttt cccatataca tatatcccaa aaattcggta
      601 attgaaaacc cgttggccag ttcatcgtaa ccttttttat aatcgccacg gagcggggga
      661 acggtgttgt ttgggttggt gatttttatt ttatgctgca caaatcccgg ctgcagcttt
      721 atcaggatac cgctgttggg attggattta aacaccggaa tgaccttgcc ggccttaaga
      781 ctgacagcaa aaccctgttc gctgaaaaac acatcggcat attgtccgtc agcagtgata
      841 aggaaactat ccgaagtggc aatattatcc agaatgccaa actgatcttt caccacattg
      901 ccaaaaaggt aattaccctc caccccgaac aaccagtttt tttttgtttt tatctgaaaa
      961 ccaccgccga tcgccgagtt gccgccaaac ctgctggcaa ggtccccgcc cggtagctga
     1021 taagcatacg tggcataaag catagtggaa aatatacagg aatcattaac actaacctgc
     1081 gggcgtgcct gagagcataa aaaaagaaaa accaaaaaaa ataataattt cttcatcttc
     1141 atttctgacg ccaaaattaa ctaaaaaaca gtctgtataa tttaacagtc cagaaataat
     1201 attattgccc agaaataatt ttttttgggc atcgcaaaca ttatcatttt gactacctgc
     1261 gtattcttaa aaaacatatc tttgcaactt aaaaaaaatt cgatatcata taaacaattc
     1321 gacatatgca caaaatcact atggttgacc taaagcgtca atacgaggat atcaaacacg
     1381 aaatagatac tgccattcat gaagtaattg attccacagc ttttatcaac ggtcctgctg
     1441 taaaccggtt tcaaaaaaac ctagaagcct atcttggcgt aaaacatgta atcccttgtg
     1501 ccaacggcac ggatgcgtta caggtttcga tgatggcatt gggattaaaa ccgggcgacg
     1561 aagtcatcac tacctctttt actttcatag ccaccgctga agtaattgcc ctgctgggcc
     1621 tgactccggt tctggtggat gttgaccccg acacttttaa tattgaccct gtggccatag
     1681 aacgcgccat cacacccaaa accaaagcta tcgtcccggt gcaccttttc ggacagtcgg
     1741 ccgatatgga agccatcacc gctatcgcag aaaaatacca acttcatata attgaagaca
     1801 actgccaggc cattggagcc gactatattt ttgccgatgg acacagaaaa aaaacaggtg
     1861 gtattggcat cgtgggatgc acatcatttt tcccatccaa aaatctggga tgttacggtg
     1921 acggcggagc cgtatttacc aacgacgaca acctggccac caaactgcgg caaattgtaa
     1981 atcacggcat gacggttcgg tattaccatg acatgatagg cgtaaattcg cggctcgaca
     2041 gtattcaggc agcaattctt gacataaaac tcaaacacct cgacgagtac gctcaacgcc
     2101 gcaatcaggc cgcctcatac tacgaccagg catttgcagc agaaccacgc cttaaaacac
     2161 cggtacgctt caaaaaatcc acccatgtct ttcatcaata cacgctggta acacaagata
     2221 ccgacagagg caaacttcag gatttcctga acaccaaaaa tgtccctgcc atgatctatt
     2281 atcccgttcc gctgcatatg cagaaagctt acctcgaccc cagataccgg caaggcgatt
     2341 tcccggtaac ggaaatgtta tgcaaatcgg taatatcact gcctatgcac acccatcttg
     2401 acaacgagca acttgagtac attaccgctt cggtactgga atttttcaaa caataatcct
     2461 atggaaaaag acttttttgc acatgaaaca gccatcattg atcagggctg cacaatagga
     2521 aaaggaagca aaatatggca tttcagccac atgatgaccg gttgtgaact gggcgaaaat
     2581 tgtaatatcg gacaaaatgt cgtggtttca cctggtgtaa aactgggcag aaacgtaaaa
     2641 gttcagaaca atgtatccat ttataccggc gtaatttgcg aagatgatgt ttttctgggg
     2701 ccttccatgg tttttaccaa tatcattaat ccccggagtg ctgttgtacg caaggatcaa
     2761 tacgttaaaa ccgtagtaaa aaaaggtgcc agcattgggg ccaatgccac tatcgtctgc
     2821 ggacacgata tcggagaata cgcctttatt ggcgcgggag cagttgtcac caaggaagtt
     2881 cccgcctttg ccctcgtggt ggggaacccc gccaaacaaa taggatggat cagcgaatac
     2941 ggacaccgcc ttaatttcaa tgaagaggga attgccgtgt gtcctgaaag caatgaaaaa
     3001 tacaagttcc ataatggaga agtttctaaa ttagcatgac atgaaaaaat ttttcttact
     3061 aatcgcctgt tgttgcatga gtattttttt tacatcatgc gaagaatcca actgcgacaa
     3121 ctgtactatc caaaaacgtg aattttgcct gatgatgttt gaggtcaatt gtaacagcgc
     3181 tgcactgaca accaatatgg attatctggt gcaggcctgc ggacaggatg aagccagtca
     3241 gtggatatca gctgttaccg acagttgtac ccagggaatt cttttatgtc ctgctgaatg
     3301 tcaataagat tcatctcatg aaaaa
//
LOCUS       DAOSFP010000260         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_26852_length_3322_cov_4.157943, whole genome shotgun sequence.
ACCESSION   DAOSFP010000260 DAOSFP010000000
VERSION     DAOSFP010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26852_length_3322_cov_4.157943"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2486)
                     /locus_tag="PLI16_07825"
     CDS             complement(<1..2486)
                     /locus_tag="PLI16_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84505.1"
                     /translation="MRNFRLVLLLIISLIILIACQTLSYSQVASFPWSNNNGIPYHKS
                     FDRCTGFEAFIPVQHKKIAFLLKEESVIRIFASEKKLKHQDIAINAPAYDFDYHKQKF
                     FLLSPGAVEIIDLSKGTRQLIPMKTSPVFFSKIKVVGGKIFLFTNNQETYELQPETAK
                     IQKVPDQGMIMQEGVSLRSVKNSENSFSISINNHTKTYNTTQKLASAQIAGYNGSCIF
                     VDVQYIVHEVPLKVAREIWSIKTGKNGIIREPVIRKVPNCYYILAASDISITPTKAYY
                     ALTDPEGVKIFNLNANTEITTKKEFLDIHFSSNTMPAPELQDIGHAVKAEDSISRPEI
                     IANAEPFATHAWYCNAGNIYDNVSCGGTTINTPSWITTGPKTSIPYCWGGWTSLTVFD
                     AELLNNKSAGDDATSYSSSSPQCAVGVDCSGFVSRVWELSYKRGTSTLPNVSTAYASF
                     SQLLPGDLINYAGSHARLFHSWNLSGTMHIIESSASGTNWRVGYNDYTAAQLQANYIP
                     RYYNYIINSVPAPQDTTPPQTSITAGTWHTGNFNVSFTDTDASGIQERYWLPASLNNN
                     NWHASTADGFLYEDYTDTVNVIWTTIEPSWNIISGALCQTNQALTQNNAYTFLKQDSN
                     NSYLFSWKMKVSGTSSDRRGGIYFFSDSGAMLQRNNAYMVYYRADYDKCQIYKSVNNA
                     ITLMTDDEAVIDENGWFDCDVTYQPASGLISAYLNKELVSSWVDPAPLTSGRYVSLRT
                     GACQGYFDEIRAYKSRANSSLVTVGPGKMIPDQNPNPSTPACRIHSVVIDSANNMFST
                     FSATNIDYTPPTPVPAVADYHPGDADTTSEA"
     gene            complement(2605..>3322)
                     /locus_tag="PLI16_07830"
     CDS             complement(2605..>3322)
                     /locus_tag="PLI16_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOH84506.1"
                     /translation="PVIIGITQAETKTVFTETASKNNSKIIFADTLLHLENFRFMQEP
                     PFGSISNIRFSEKPFLNEVYCALSGIYQQENIKTVVAACLELAECGYPIDEKTLRNGL
                     KNVVKNTGLQGRWQVLSTTPLTICDTGHNKDGLTQVIRHINLIKHNNLHFVFGLVSDK
                     DVAAILGLLPADATYYFCKPDIPRGLDVTTLIKEAEKQGLKGKSFDSVGQALKSAQNA
                     AKENDLVFVGGSTFVVAEVL"
BASE COUNT          819 a          703 c          809 g          991 t
ORIGIN      
        1 gcttcggagg tggtgtcggc atcgccggga tgatagtctg ccactgccgg aacaggtgtt
       61 ggtggcgtat agtcgatgtt ggtggcggaa aaagttgaaa acatattgtt agccgagtca
      121 atcaccaccg aatggatgcg gcaggccggg gtggaggggt tgggattttg gtccggtatc
      181 attttccctg gtccgaccgt aaccagcgaa gagtttgccc tgcttttgta cgctctgatt
      241 tcgtcgaaat atccctggca ggctcccgtt ctcagcgaaa catagcgtcc cgaagtcagg
      301 ggcgcaggat cgacccatga agaaaccaat tccttattca gataagctga gataagcccg
      361 cttgcaggct gataagtaac atcgcaatca aaccatccgt tttcatcgat gacggcttcg
      421 tcgtctgtca tcagggtgat ggcgttgttt acggatttat aaatctgaca cttgtcgtaa
      481 tcggcacggt aatacaccat ataggcgttg ttgcgttgaa gcattgctcc gctgtcgctg
      541 aagaaataaa tacctcctct tctgtcgctg ctggtgccgc ttactttcat cttccagcta
      601 aacaggtagc tgttgttgct gtcttgttta agaaaagtgt acgcgttgtt ttgggttaat
      661 gcctgatttg tctggcatag ggcgcctgaa attatgttcc aggagggttc aatcgtcgtc
      721 cagatgacat taacggtatc ggtgtagtcc tcatataaaa atccgtcggc tgtgctggca
      781 tgccagttgt tgttgttcag cgatgcagga agccagtatc tttcctgtat tcccgatgcg
      841 tcggtatcgg tgaacgacac attgaaattt ccggtatgcc aggtgcctgc tgtaatggag
      901 gtctgtggag gcgtggtgtc ctggggcgca ggaaccgaat tgataatata gttataatac
      961 cgcggaatat aattagcctg aagctgagcg gcagtatagt cgttatatcc gacgcgccag
     1021 ttagtgcccg aagcggaaga ttcaatgatg tgcattgttc cgctcaggtt ccaggaatga
     1081 aacagccggg catggctgcc ggcgtaatta atcagatcgc ctggtaaaag ctggctgaaa
     1141 ctggcatagg cagtggaaac attcggcagt gtggaggtgc cacgtttgta agaaagttcc
     1201 cacacccgtg aaacaaatcc cgaacaatca actcctacgg cacattgcgg acttgaagag
     1261 ctgtaagatg tagcatcgtc gcctgcagat ttattgttta acagttctgc atcaaacacg
     1321 gtcaggcttg tccagcctcc ccagcaatag ggaatagagg tcttagggcc tgttgttatc
     1381 caggaaggag tgttaatggt ggtgccaccg caacttacgt tgtcatagat attgccggca
     1441 ttgcaatacc atgcgtgagt ggcaaaaggt tccgcattgg caatgatctc cgggcggctg
     1501 atagagtctt ctgccttcac cgcatgacca atatcctgta actcaggcgc aggcatcgtg
     1561 ttgctactga aatgaatatc cagaaattcc ttttttgtag taatttctgt gttagcattc
     1621 agattgaata ttttcactcc ttcggggtcc gtaagggcat aatatgcctt tgttggtgtg
     1681 atgctgatgt cgctggctgc taatatgtaa tagcagtttg ggaccttccg gatcaccggc
     1741 tcacggataa tgccgttttt accagtttta attgaccata tttcacgggc caccttcaag
     1801 gggacttcat gaacgatgta ctgcacatcc acaaatatgc aggagccatt ataccctgct
     1861 atttgagccg aagctaattt ttgggtggtg ttgtaagtct tcgtgtggtt gttgatactt
     1921 attgagaaac tgttttctga attttttacc gatcttagag aaacaccttc ttgcattatc
     1981 ataccctggt ccggtacttt ctgaatttta gctgtttcag gttgcagctc ataggtctcc
     2041 tgattattgg tgaacaagaa aatttttccg ccaacgactt tgatttttga aaaaaatacc
     2101 ggtgaggttt tcatgggaat aagctggcgg gtgcctttcg ataggtcgat gatttcaact
     2161 gcaccgggag ataaaagaaa gaatttttgt ttatgataat caaagtcgta agcaggtgcg
     2221 tttatggcaa tatcctgatg tttcagtttt ttctctgaag cgaaaattct tatcacgctc
     2281 tcttctttta aaagaaaagc gatttttttg tgttgtaccg gaataaaggc ctcaaaccct
     2341 gtgcagcggt cgaacgattt gtggtacggt atgccgttat tgttgctcca ggggaaggag
     2401 gctacctgtg aatatgaaag ggtttgacag gcaattaata ttatcaggct gataatcagc
     2461 aaaagtacaa gacgaaaatt tctcatggtt ttttattatt tccggacgca taaaaatacc
     2521 aaaaatcctt ttaaaaccag cgttataaaa tagtttataa aagtttagtt tccacttgtc
     2581 catccggatg attaaaaaac acgatcataa aacttcggcc accacaaaag tgcttccgcc
     2641 cacaaatacc aggtcgtttt ctttcgcagc gttttgtgct gattttaaag cctgtcctac
     2701 cgaatcaaaa ctttttccct tcaggccctg tttttctgct tcttttatta gtgttgttac
     2761 atccaggccc cgtggaatat cgggtttgca aaaataatac gtggcgtccg caggcaacag
     2821 cccgagtatg gctgccacat ctttatcgct gaccagtccg aaaacaaaat gtaaattatt
     2881 gtgtttaatg aggtttatat gccggattac ctgtgtcagc ccgtctttgt tgtgtcctgt
     2941 gtcgcagatg gtgagggggg ttgtggataa tacctgccag cgtccctgca aaccggtgtt
     3001 tttcacaaca tttttcaatc cattgcgcag ggttttttca tcaatagggt atccgcattc
     3061 cgctaattcc aggcaggcgg ctaccacggt cttaatgttt tcctgctgat agataccgct
     3121 caatgcgcag taaacttcat tcaggaaagg tttttcgcta aaccggatat tgctgatgct
     3181 tccgaacgga ggctcctgca taaaacgaaa attttccagg tgcagaagtg tatcggcaaa
     3241 gataattttg ctgttatttt ttgaagcggt ttcggtaaat accgttttgg tttctgcctg
     3301 cgttattcca atgattacag gt
//
LOCUS       DAOSFP010000261         3317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_26918_length_3317_cov_3.657265, whole genome shotgun sequence.
ACCESSION   DAOSFP010000261 DAOSFP010000000
VERSION     DAOSFP010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26918_length_3317_cov_3.657265"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            438..1412
                     /locus_tag="PLI16_07835"
     CDS             438..1412
                     /locus_tag="PLI16_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84507.1"
                     /translation="MKVAIVFVLCLCLFNIALRGQNVSPGDTITEEMQGLILSKKGDK
                     INVLMNDSATKMPVLNMVGELSKSFEKEILGTTVTGWLTIGIVRVVAIKERIIRLAVL
                     EEKSIITIDGVKQDQFTPGQTIKLTWKMLVSPDEILFRKGLQEYDNSKPSAINYFRQA
                     TIINPKHAEAFNMIGIIHHQNNIFDSAAIYYKKAWMLDTSNITYIKNIAVASTNLNLY
                     SEAYLFAEKAVFHSPDDAEAHYLRAITYLYAIIYSWKGEMTAIQKKQILSDMDFAVTT
                     NENDSFYYKERMIIRSFFLDYAGACEDAKKYRALEGSAGDAFVIEYCY"
     gene            1421..2035
                     /locus_tag="PLI16_07840"
     CDS             1421..2035
                     /locus_tag="PLI16_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOH84508.1"
                     /translation="MMKKITLLIILSFVLSSSYAQKFGGGFFAGIAASQISGDQLSGF
                     YKAGPYVGAFSNFAFDEQSALQLELYYILKGSSKNQRPKINDYTIYKLNLHYIELSLL
                     YKWQFSKRFWLETGPALGVLMKNTGIEKDEFGTVYDSQRPRFNKFDFSGLLGLGINII
                     DHLKANIRYQNSIIPVRGSEIPYWRIQKRWQFSSSITISAIYEI"
     gene            2032..2604
                     /locus_tag="PLI16_07845"
     CDS             2032..2604
                     /locus_tag="PLI16_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOH84509.1"
                     /translation="MIRKKIVIGITGASGAIYAENLVNRLHQHQDQVAELGIVLSENA
                     VKVRQHELGLPANSFAAGRIYDNKDFFAPFASGSSRYDVMIICPCTVGTLGRIAHGYA
                     DDLIARTADVMLKENKKLILLLREMPFNLVHIENIKLLCQSGAIICPSSPSFYSRPQN
                     IEELINTVTDKLLLLAGFEIKSYEWGNDKP"
     gene            complement(2615..2950)
                     /locus_tag="PLI16_07850"
     CDS             complement(2615..2950)
                     /locus_tag="PLI16_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84510.1"
                     /translation="MEQKKRVIIHINNIGPEVLEALHNKYPEGYQDRIFKVTKPNGDF
                     FHAITVDTKDASYLIKVDVKIDTATDEKIEEQLFAKDDIPDADIKASDEDDTEDAGKM
                     PADDDDIAI"
     gene            complement(3062..>3317)
                     /locus_tag="PLI16_07855"
     CDS             complement(3062..>3317)
                     /locus_tag="PLI16_07855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH84511.1"
                     /translation="TPDRDGNNEEFLVKGTGILNSTFNMIIYDRWGKQVFQSNDVEKG
                     WNGKINETTVADPAVFTWVVTYRDIDRKYHKYTGSLVLLR"
BASE COUNT         1015 a          687 c          612 g         1003 t
ORIGIN      
        1 aaactgttca ttaaatatga caatgttgcc ttgttatatt tactgataag tttcatggtt
       61 ttatcctcct gtttttatgt ttataaattt ttaagttttc aaaacagttt tctcaaactc
      121 tatgcaaaat ttggtaaaaa agacgacgta ttttttaaac aacttattat caacacattg
      181 cgttaatttc tttcaaaaac acagccagcc atattgtcag ttacgacaca taaaacaatg
      241 ccatattgac gccttgtttt tatcccatac tactatatcg ttgaatatgt gctaattaac
      301 aaaatttctt attttacgat gttgtcaata ccttttcgac tacaaaactg ctggtgtcta
      361 tacttgtagg agccccagtt ttatgcaaat aatatggaaa tatgcttatt tttttgtatc
      421 tttacaaaaa caatacgatg aaggttgcga ttgtttttgt tttatgtttg tgccttttca
      481 acatagcgtt gcgtggccaa aatgtaagtc cgggtgatac catcaccgaa gaaatgcaag
      541 gacttatcct gtcgaaaaaa ggcgataaaa tcaatgtgtt aatgaatgat tccgccacaa
      601 aaatgcccgt tttaaacatg gtgggagaat tgtcgaaaag ttttgaaaaa gaaatcctgg
      661 gcaccactgt taccggttgg cttaccattg gcatagtaag agttgtcgct atcaaagaac
      721 ggattatcag gttggcagtt ttggaagaaa aaagcattat caccatcgac ggggtaaaac
      781 aagaccaatt cactccggga caaacaataa agcttacatg gaaaatgctt gtcagccccg
      841 acgaaatact tttccgcaaa gggttacaag agtacgacaa cagcaagcct tcagccataa
      901 actatttcag gcaggccacc atcatcaatc caaaacatgc agaagccttc aacatgattg
      961 gcataataca tcatcaaaac aatattttcg attctgcggc catatattac aaaaaggcct
     1021 ggatgcttga cacatcaaac ataacctata tcaaaaacat agctgttgcc agcaccaacc
     1081 ttaacctgta ctccgaagca tatctctttg ccgaaaaagc agtttttcat tctcctgatg
     1141 atgcagaagc gcattatctg agagcaatta cttatcttta cgccattatc tattcatgga
     1201 aaggagaaat gactgcaatc caaaaaaaac agattttatc agatatggat tttgccgtaa
     1261 ccaccaatga aaacgactct ttttattaca aggaacgcat gataataaga agcttttttc
     1321 tggattatgc cggcgcctgc gaagatgcca aaaagtaccg ggcgctggaa ggcagtgccg
     1381 gcgatgcatt tgttattgag tattgctatt aatataaaaa atgatgaaga aaattacgct
     1441 gctgataatt ttatcgtttg tcctttcatc ttcttatgcc caaaaattcg ggggcggttt
     1501 ttttgccggc atagcggctt cgcagatttc cggcgatcag ctttcaggat tttacaaagc
     1561 cggaccctat gtgggagcct tttccaactt tgcttttgat gaacaatcgg cactacaact
     1621 cgaattgtat tacattttaa aaggcagcag caaaaatcag cggccaaaaa tcaatgatta
     1681 caccatctat aagctaaatc tgcattacat cgaattaagc ctgttgtaca aatggcagtt
     1741 ctcaaagcgg ttttggcttg aaaccggacc tgccctgggc gtactgatga aaaacaccgg
     1801 cattgaaaaa gacgaatttg gcaccgtgta cgattcgcag cggccccgtt tcaacaagtt
     1861 tgacttcagc ggccttttag gcttgggaat caacattatc gaccatctga aagccaatat
     1921 acgctatcaa aactccatta ttccggtccg cggttcggaa attccatact ggagaattca
     1981 aaaaaggtgg caattcagca gcagtatcac catatccgct atttacgaga tatgatcaga
     2041 aaaaaaattg ttatcgggat aacaggtgcc agtggcgcaa tatatgcaga aaaccttgtc
     2101 aacaggctgc atcagcacca ggaccaggtt gccgaattgg gcattgtgct ttctgaaaat
     2161 gccgttaaag tcaggcagca cgaactcggc ttgccggcca actcttttgc cgcgggcagg
     2221 atatatgaca ataaggattt ttttgcacct tttgcttcgg gttcgtcacg atacgatgtc
     2281 atgattatct gcccctgcac cgtgggaacc ctcgggcgga ttgcccacgg ttatgccgac
     2341 gacctgattg cacgcacagc agacgttatg ctgaaagaaa acaaaaaact tatcttgctg
     2401 ttgcgagaaa tgcctttcaa cctggtacat attgaaaata tcaaactatt atgccaatcc
     2461 ggcgcaatca tttgcccttc atcaccatct ttttattcca ggccccaaaa tatcgaagaa
     2521 ttaataaata cagtaaccga caagctgtta ttgttggcag gttttgaaat aaaaagttat
     2581 gaatggggta acgataaacc ttaaaagttt cctgctatat agctatatca tcatcgtcag
     2641 cgggcatttt tccggcatct tcagtgtcgt cttcatcaga ggccttaata tctgcatcag
     2701 gaatatcgtc tttggcaaat aattgttctt caattttctc atcggtagct gtgtcaattt
     2761 ttacgtctac cttgataaga tatgaagcat cctttgtgtc aacggttatg gcatgaaaga
     2821 aatcgccatt aggttttgtt actttgaaga ttctatcctg atatccttcc gggtacttat
     2881 tatgtaaggc ttctaaaact tccggtccga tgttattgat atgaataata actctctttt
     2941 tctgttccat ataaaatcaa attcaaaatt gccagttcca tcaaaaaatg taccgatctg
     3001 caaaataaaa gttttcaaaa ataatcattt tgcattttgc aaaatattct tttcaaaaaa
     3061 atcaccttaa taatactaac gatccggtat atttatggta tttcctgtcg atatcgcggt
     3121 aggtcaccac ccaggtaaat acggcggggt cggctactgt agtttcattg atttttccat
     3181 tccagccttt ttctacatcg tttgattgaa aaacctgttt tccccaacgg tcataaataa
     3241 tcatgttaaa ggtactattg agtattcctg ttcctttaac caaaaattcc tcgttgtttc
     3301 catccctgtc aggagta
//
LOCUS       DAOSFP010000262         3308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27006_length_3308_cov_3.576698, whole genome shotgun sequence.
ACCESSION   DAOSFP010000262 DAOSFP010000000
VERSION     DAOSFP010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27006_length_3308_cov_3.576698"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLI16_07860"
     CDS             <1..263
                     /locus_tag="PLI16_07860"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="MDM; functions in conversion of succinate to
                     propionate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOH84512.1"
                     /translation="KQAVENDVHILGVSSLAAGHKTLVPQVIEELKKLGREDIMVIVG
                     GVIPPQDYDFLYKAGAIAIFGPGTKIPEAAIKILEILIEARK"
     gene            373..753
                     /locus_tag="PLI16_07865"
     CDS             373..753
                     /locus_tag="PLI16_07865"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH84513.1"
                     /translation="MNRFITFLLAVFIGMPVVFGQTTIKGKVFSKTDGKAIAGATVIA
                     KDSPEILTLSDSEGNFKIKVPHGTAALEVNAAGYTSKTRGIEGKRKVNFELAPASTDP
                     RINMMKSRHIKISQPATIDEKLTK"
     gene            complement(763..1605)
                     /locus_tag="PLI16_07870"
     CDS             complement(763..1605)
                     /locus_tag="PLI16_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84514.1"
                     /translation="MKKNKFIWTFALPFLGVVPIVLIAAFEPKIVSRWGEPVWNTLFA
                     VAMVLVFVLSFLPFIGLFKNLWGKKGFNFFWGRGKLAGQILVAGSDATATIVSIGENS
                     KGGIVTINDQPLLNLVLRIDNNYDPEYEVSFDTIMPRTAVAQFQPGAQFAVKVDRADK
                     NNVVFNAQATQTMTGSGQKPTYGGKGWTAQDHLRVEQAGIDGMAKLMSVEDTGKSEDF
                     NPVVMIGYEVSLHGETSYFFEKEIAMPTETVLKLKTLTGKSFKARVHPDDRTKITVDF
                     TFAD"
     gene            1723..3210
                     /locus_tag="PLI16_07875"
     CDS             1723..3210
                     /locus_tag="PLI16_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH84515.1"
                     /translation="MKKITALIIIVYVLTMKGAAQTNTDAEKGLQQISVTEMMKDIEF
                     FASPKLEGRFPGSKGYDKAAGYSAEYFKNIGLTPYSHNSYIQPVPVEYNEFTGAVKLE
                     KIQQGKAVKQFTHGSDFVCRGFSGTGNITAPVVFCGYGMTFPELGYDDYASADVKDKI
                     VLVFKQNPSWANNIEGINMMLTRYKAGNAAKHGAKAILFVSTPNMANPQKPIGSIMDG
                     TGEQLPDFPQIQISLELANELLMASEKNLSSLQAWIDSLKKPVSIEINDTMHLQVNTK
                     YEKEHFSKNIIAVLPGSDAKLKDEYLVIGAHLDHVGKQGNIFFPGANDNASGSAAVLQ
                     IARTFATAEIKPKRSIIFVLFTSEEQGLNGSRFFTDNPPAPLEKIIAMFNLDCIGHGD
                     SIQVGGGSTVPLLWHAARRQDSLHTRLMTAKTWPGGGSDAQPFFENGIPTLYFATKNS
                     YTHLHLTTDTPPTLNTELLEKVTRLVFLTAFEIAGGNYSKELLVK"
BASE COUNT          941 a          809 c          729 g          829 t
ORIGIN      
        1 ccaaacaagc cgtagaaaac gacgtgcaca tactcggcgt ttccagtctg gctgccggcc
       61 acaaaacatt ggtgccacag gtaattgaag aactaaagaa actgggacgt gaagacatca
      121 tggtcattgt tggaggcgtt atccctccgc aggattatga ttttctttac aaggccggag
      181 ccatcgccat ttttggaccg ggaaccaaaa ttcctgaagc cgccatcaaa atactggaga
      241 tattaataga agccaggaaa taagtttcct gcattcacat gttccggttt tcattttgtg
      301 ttcgctgtgt tttttggctg ccagtgtttg gcgtgaaatt tgcctatgtg acatgttaaa
      361 aatgtaagca ccatgaaccg tttcatcacc ttcttacttg ccgtattcat tggaatgccc
      421 gtggttttcg gacaaactac catcaagggg aaagttttct caaagactga tggcaaggcc
      481 attgccggag ctactgtcat tgcaaaagat tcgcccgaaa tccttacgtt aagtgactca
      541 gaaggaaact tcaaaataaa agtcccgcac ggcaccgcag cactcgaagt gaatgcggca
      601 ggttatacct caaaaaccag aggcattgaa ggcaaaagaa aagtgaattt cgaactggca
      661 cctgccagca ctgaccccag gataaacatg atgaaatcac gtcatataaa gatttcgcaa
      721 cccgccacca ttgacgaaaa gttgacaaag tagcctgccg gcctaatccg caaaggtaaa
      781 atctacggtg atttttgtcc tgtcatcggg atgcaccctg gctttaaagc tttttccggt
      841 caatgtcttc agttttaata ccgtttcggt aggcatggct atttcttttt cgaaaaagta
      901 agatgtttct ccgtgcaggg acacctcata gccaatcatc accaccgggt tgaaatcttc
      961 gctttttccg gtgtcttcaa cactcataag tttggccatg ccgtcgatac cggcctgctc
     1021 caccctcaga tggtcctggg ctgtccaacc ttttccgcca taggtgggct tttgacctga
     1081 tccggtcatg gtttgggtag cctgggcatt aaacaccaca ttgtttttgt cggctctgtc
     1141 cactttgacg gcaaactgcg ctcccggctg aaattgcgcc actgccgtac ggggcatgat
     1201 ggtatcaaaa ctcacctcat attcaggatc atagttgtta tcaatacgta aaaccaggtt
     1261 caacagcggc tggtcgttga tagtaactat cccacccttg ctgttttcgc cgatacttac
     1321 aatggtagcc gtggcatcgc tgcctgccac cagtatctgt cctgccagtt ttcccctgcc
     1381 ccagaaaaag ttgaaacctt tttttcccca caggttctta aacaagccga taaagggtaa
     1441 gaacgaaagc acaaaaacca gcaccatagc cacagcaaac agggtgttcc acaccggttc
     1501 gccccacctg gaaacaattt tgggctcaaa tgccgcaatc agcactatgg ggacaacacc
     1561 aagaaaaggc aaggcaaaag tccagataaa cttatttttt ttcataatcg acggatattt
     1621 gatattacaa tttcaatata tggttaatta cctgcttcct cttcatcaat ctttgtaatt
     1681 tagcaagcct aatttacaac tttttcagat aatttacccg ccatgaaaaa aattacagcc
     1741 ctgataataa tagtttatgt cctgacgatg aaaggtgccg cccagacaaa taccgatgca
     1801 gaaaagggct tgcaacaaat ctcggtaact gaaatgatga aggatattga gtttttcgct
     1861 tctccaaaat tagagggccg ttttccaggc agcaagggat atgacaaagc agccggttat
     1921 tccgccgaat attttaaaaa tattgggttg acgccataca gccacaatag ttatatacag
     1981 cctgtccctg tagagtacaa tgaatttacc ggggctgtaa aactcgaaaa aatacagcag
     2041 ggcaaagccg tgaagcagtt tacacacggc agcgattttg tatgccgggg gttttcaggc
     2101 acagggaaca tcacggcgcc tgtggtgttt tgcggctatg gcatgacttt tccggaactt
     2161 ggttacgatg attatgcttc ggctgatgtg aaagataaaa tagtgctggt cttcaagcaa
     2221 aacccttcct gggcaaataa tatagagggc atcaatatga tgctgacccg ctataaggcc
     2281 ggcaatgcag caaaacacgg cgccaaagcc atactttttg ttagcactcc caatatggca
     2341 aatccgcaaa agcccatcgg cagcattatg gatgggacag gcgaacaact gcctgatttt
     2401 cctcagattc agatcagcct ggagttggca aacgaactgc ttatggcctc agaaaaaaat
     2461 ctcagttccc tgcaggcgtg gattgattcg ctgaaaaagc ctgtcagcat agagattaac
     2521 gacactatgc acctgcaagt gaacacgaaa tatgaaaaag aacatttttc gaaaaacatc
     2581 attgctgttt tgcccgggtc tgacgctaag ctgaaagatg aatacctggt catcggggca
     2641 catctcgacc atgtaggaaa acagggcaac atattttttc cgggcgccaa cgacaacgcc
     2701 tcgggcagtg cagccgtgct gcagatagca aggacttttg ccactgcgga aatcaaacca
     2761 aaacggtcaa taatttttgt gttgtttacc agcgaagaac aagggcttaa cggttcaaga
     2821 tttttcaccg acaatccccc ggcaccgctt gaaaaaatca ttgccatgtt caaccttgac
     2881 tgcataggcc atggcgacag catacaagtg ggaggaggaa gcacggtgcc gttgctttgg
     2941 catgcagcgc gccggcagga ctcgctgcat acgcgcctga tgactgcaaa aacctggccc
     3001 ggtggcggat ccgatgccca gccatttttt gaaaatggca tccccacctt atactttgcc
     3061 accaaaaact cgtacactca tctgcacctc accaccgaca ctcccccaac cctgaataca
     3121 gaactgctgg aaaaagtaac caggctggtt tttctaaccg ccttcgaaat tgccggtggt
     3181 aattattcaa aagagctcct ggtgaaataa tctgtaatac ctattggaaa ataattcgta
     3241 ttattttaac catcggcctg cctaccggca aggcaggcat aaccctgtca gactgtcgca
     3301 gacaggca
//
LOCUS       DAOSFP010000263         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27062_length_3304_cov_3.695291, whole genome shotgun sequence.
ACCESSION   DAOSFP010000263 DAOSFP010000000
VERSION     DAOSFP010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27062_length_3304_cov_3.695291"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..1533
                     /locus_tag="PLI16_07880"
     CDS             298..1533
                     /locus_tag="PLI16_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018572321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOH84516.1"
                     /translation="MNKVELLAPVGSFESLAAAIQGGADAVYFGVGNLNMRSRSSVNF
                     TIQDLNEIAELCRKNNMKSYLTLNTIIYDHEISEVTAILEAVKQSGIDAVIASDFCVI
                     ERARQAAIPVHISTQCNITNIDAVRFYSKFADVMVTARELHLDQVAAIVRQIEQEQIK
                     GPSGELVRIEVFAHGALCMAVSGKCYLSLHENNHSANRGACYQLCRRSYIVTDKESGT
                     ELEIDNEYIMSPKDLCTIGFLDKVLKAGATTLKIEGRGRSADYVKTVTACYKEAINSI
                     ADGTFNETKVNEWNDRLKTVYNRGFWDGYYLGQTLGEWNNVYGSQAVIEKVYVGKVTN
                     YYSKIKVADIKVETRPLSLGDEIMIIGNTTGVYEDVVKEMRVDNKPVPIVDKGIVCSI
                     PLQGEVHRGDKLYMLKAKD"
     gene            complement(1578..>3304)
                     /locus_tag="PLI16_07885"
     CDS             complement(1578..>3304)
                     /locus_tag="PLI16_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH84517.1"
                     /translation="YKDTKFNLAKAYQLNGEYDRALGLIRQYKQSLSPADLQQYSKEI
                     EKRIMECENGKKMTASPVRVFIDNLGPIVNSAFPEYSPLINADESVLFFTSRRDNTTG
                     GKRDENDLGFYEDIYMTEKINGAWTAPKNPPNPLNGSKHDATVGLSPDGQTLLIYKGV
                     NGGDIYECKLKGTAWSTPSRIGGDINTDFHESSGTISYDGKNLYFVSDRDGGFGGNDI
                     YISVKDKKGNWGKPQNLGAGINTPYNEEGVFIHPDGKTLYFSSQGHTTMGGYDIFKSV
                     FDGTKWGEPENIGYPINTPDDDVFFSISASGVHGYYSSIKQDGLGGQDIYVITFLGPE
                     KPLINNTEDNLLAGMAQPVNETVIEKPVEIKENQVTLLKGIVMDDITLEPLGATIEIT
                     DNERNEVIGTYESNSASGKYLVSLPSGKNYGIAVKAPEYLFHSENIIIPPTTTYREVE
                     KDIKLKKVAVGSTIVLKNIFFDFDKFSLRPESIPELERLLKFMTELPSVKIEISGHTD
                     NIGAAAYNKTLSENRAKAVVDYLVGKGIDKNRMTYKGFGFDQPIATNDTEEGRQLNRR
                     TEFKIISK"
BASE COUNT          822 a          709 c          800 g          973 t
ORIGIN      
        1 ctttagggat ggggtaatga tgattttcag caaaagctag ttttgataca gcctcgcctt
       61 tgctgatttt caccgtcctg ctgattcacg cctgcgccgg cccgccgttt tgactggcca
      121 ccgcgccgct attaacaatc atttgtgcta taagcataca ttttaccggc ctgtgaggaa
      181 gaaaaaccct tattaatatt gggcgcataa tgcgaatcgt aaatcaaatt gtattctaag
      241 tttaaaaaac gaaggctgtc taattgacca gatcttagta actttgcaca aaaaacaatg
      301 aataaagtcg agctgcttgc tcccgtcggg tctttcgaat ctctcgccgc agctatacag
      361 ggcggtgccg atgccgtgta ttttggtgtc ggaaatctta atatgcgttc ccggtcttcg
      421 gttaatttta cgatacagga tcttaatgaa attgctgagc tctgccgcaa aaataacatg
      481 aagtcctatc tgacgctgaa caccattatt tatgaccatg aaatcagtga ggtgacagcc
      541 attttggagg cagtgaagca aagcggtatt gatgctgtta ttgcttctga tttttgtgtg
      601 attgaacggg cccggcaagc cgctatccct gtgcatattt ctactcaatg caatatcacc
      661 aacatagatg cagtgcggtt ttactcaaaa tttgccgatg tgatggttac tgcccgtgaa
      721 ctgcatcttg accaggtggc cgccattgtc aggcagatag aacaagagca aatcaaaggc
      781 ccctccggcg agttggtcag gatagaggtg tttgcgcatg gagctttgtg tatggccgtt
      841 tcgggtaagt gctacctgag cctgcacgaa aacaaccatt cggcaaaccg tggtgcctgc
      901 tatcagctgt gccgcaggtc ttatattgtt accgataaag aaagcggaac cgaactggag
      961 attgacaatg aatacatcat gtcgcccaaa gacctctgca ctataggctt tttggataaa
     1021 gtgcttaagg ccggcgccac aaccttaaaa attgaaggcc gcggcaggtc ggcagattat
     1081 gttaaaacag ttacggcgtg ttataaagaa gccataaaca gtattgccga tggcacattt
     1141 aatgagacaa aagtcaatga atggaatgac agattgaaaa ccgtgtacaa ccggggcttc
     1201 tgggatggct attatttggg acaaacactg ggagaatgga acaacgttta tggctcgcag
     1261 gccgtcattg aaaaagtata tgtcggaaaa gtaaccaatt attattcaaa aattaaggtg
     1321 gctgatatca aagtagaaac ccgccccttg tctttgggtg atgaaataat gattatcggt
     1381 aataccaccg gtgtttatga agatgttgta aaagaaatga gggtggacaa caagcctgtg
     1441 cccatagtgg ataaaggaat tgtatgttcg attccactac agggcgaagt gcaccgggga
     1501 gataaattgt atatgctgaa agccaaggat tagttggttt cgcgaagatg cataccgccc
     1561 aacaggtcag cagttgatta tttgctgata atcttaaact cggttctccg gtttagttgt
     1621 cttccttctt ctgtatcatt ggtggcaata ggctgatcga aaccgaaacc cttgtaagtc
     1681 atcctgtttt tgtcaatccc tttgcccacc aggtaatcaa ccacggcctt tgccctgttt
     1741 tcagagagtg ttttgttata tgctgcagcc ccaatgttgt cggtatgtcc cgagatttca
     1801 attttcacgc tgggcagttc cgtcatgaat tttaacaggc gttccaactc gggaatcgac
     1861 tccggacgca gtgaaaattt gtcaaaatca aaaaagatat ttttcaacac tatggtgctg
     1921 ccaacagcta ctttttttag tttgatgtct ttttccactt cgcggtaggt agtggtagga
     1981 gggatgatga tattttcgga atggaacagg tattcggggg cttttacggc gatgccgtag
     2041 ttttttcctg atggcagcga taccaggtat tttcctgagg cactgttcga ctcgtaggtg
     2101 cctatcacct cattgcgttc attgtcggta atttcaatgg tggcgcctaa gggttccagg
     2161 gtaatgtcat ccataacaat tcctttgagc agcgttacct ggttttcttt tatttccacg
     2221 ggtttttcta tcacggtttc gtttacaggt tgtgccatgc cggccaggag gttgtcttcg
     2281 gtgttgttta ttaaaggttt ttcggggcct aagaaggtga tgacataaat atcctgtcct
     2341 cccagcccat cctgttttat agatgaataa tacccatgga cgccacttgc tgatatcgag
     2401 aaaaatacgt cgtcatcggg ggtgttgata gggtatccta tgttttcggg ctcaccccat
     2461 ttggttccgt caaaaaccga cttgaagatg tcatatccac ccatggtggt atggccctgc
     2521 gaactgaaat acagggtttt tccatcagga tgaataaaca cgccttcttc gttgtagggg
     2581 gtgttgattc ccgcgccgag gttttggggt ttgccccagt ttcctttttt gtcctttacg
     2641 gaaatataga tgtcgttgcc accaaagcca ccatcacggt cgctgacaaa ataaaggttt
     2701 ttaccatcgt aagaaatagt gcccgacgat tcatgaaagt ctgtattaat gtcgccacct
     2761 atgcgcgatg gggttgacca ggcggttcct ttcagtttgc attcgtaaat atctccgccg
     2821 ttgacacctt tgtaaatcag cagggtttgc ccgtcgggcg aaaggcccac ggtggcatcg
     2881 tgcttgctgc cgttcagggg gttgggggga tttttcggtg ccgtccacgc cccattgatt
     2941 ttttcagtca tataaatgtc ttcgtagaat cccaagtcgt tctcatcgcg tttgcctccg
     3001 gtggtattgt cgcgtcttga ggtaaaaaac aacaccgact catcagcgtt tatcagggga
     3061 gaatattcgg ggaatgccga attgactatg ggccccagat tgtcgatgaa tacacgcacc
     3121 ggcgaggcag tcattttttt gccgttttcg cattccataa ttctcttttc aatttctttt
     3181 gaatattgct gaaggtcagc gggcgataac gattgcttat attgcctgat gagacccagt
     3241 gcccggtcgt attcaccatt cagttgatag gccttggcca ggttaaattt ggtgtctttg
     3301 tatt
//
LOCUS       DAOSFP010000264         3291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27218_length_3291_cov_3.453646, whole genome shotgun sequence.
ACCESSION   DAOSFP010000264 DAOSFP010000000
VERSION     DAOSFP010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27218_length_3291_cov_3.453646"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLI16_07890"
     CDS             <1..471
                     /locus_tag="PLI16_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84518.1"
                     /translation="YSALTGLPEKRLKTIVFALNGSKKNKAETWGVNKIRINPEAYTS
                     ADDPAWIGTLAHELTHEYHYFKSLLPDCIIRFFDQFGIKFRQYILGMPHEKAYKASRM
                     EKQAFAVQAIIMDFVKTYTPQKTLADNTLSEQEKVQLLLNMAEEYKLKKWRGSR"
     gene            complement(620..2368)
                     /locus_tag="PLI16_07895"
     CDS             complement(620..2368)
                     /locus_tag="PLI16_07895"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOH84519.1"
                     /translation="MNKTISINIGGLFFQIDEEAYKLLENYLDSLKNHFANTEGKEEI
                     LQDIESRIAEIFQVKIENGAPIIVPADVEEAIKILGKPDDIGSSEESKEGKQEKSSPF
                     KKRLYRDEDNKVLGGVCSGLGHYFNVDPVWFRIGFIVALFIFGSSALVYLILWIIIPK
                     ATTTEEKLNMKGEPMTISEIQENIKSEFEELKARFKEMKGDAKNHTRREYNDMKRRMH
                     RAKRESAYSNRQRQMAAAISRTQSVKESGTGVGSVFGEIIYYFLRALLIFAGIVLLII
                     GIVVTVSLVLSITAPDSFLFFTKWGISSVSFPAFSQVFFENPWQQHMAFLSLILLIGV
                     PLLMLIFNSIRMIAGFKTKIRVVSITASLLWLTGLILAIILTINIVGNFSEKSIYRQE
                     VMLPQTRETLVIDVADNLPLVNKTQYFDDDFFDSDPGDHEHFVFNNCFFSDGNDKLVV
                     YGFPKLKIIPSKTGTYKLTVIKFSRGTTLMNARRRAESIRFDTPAADSVLLINNYFLL
                     AAGEKWRNQSVKLILEVPEGKSVYLSKNLENLVYDRDNLDGSWDFDMLGKKMYMVNGR
                     LTGDVVVVTDTIKNPK"
     gene            complement(2390..2716)
                     /locus_tag="PLI16_07900"
     CDS             complement(2390..2716)
                     /locus_tag="PLI16_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOH84520.1"
                     /translation="MDIENSKAQMRKGILEYCILSVIAEKDVYASDIMEKLKEGKLIV
                     VEGTLYPLMTRLKNDGLLNYRWEESKTGPPRKYYTLTEAGRIFLQNLDESWEELVKSV
                     KLITKK"
     gene            3015..>3291
                     /locus_tag="PLI16_07905"
     CDS             3015..>3291
                     /locus_tag="PLI16_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84521.1"
                     /translation="MDKEAKAVIIWITVCVAFVILALVLLFVPWSIPREMEVKFALIL
                     TGMLSLIIITGLTKKKYIHEAASVLNVLQYIMIALLCAVVFTGFLFWF"
BASE COUNT          917 a          736 c          652 g          986 t
ORIGIN      
        1 tattcagcgc tgacgggcct gcctgagaaa agattgaaaa ccatagtttt tgcgctcaac
       61 ggttcaaaaa aaaacaaggc ggaaacctgg ggagtaaaca aaattagaat caaccccgaa
      121 gcttacacca gtgcagatga tcccgcatgg attggaaccc ttgcccacga gctgactcac
      181 gaataccact attttaaaag cctacttccc gattgtatta tcaggttttt cgaccagttc
      241 ggcataaagt tcaggcagta tattctcggt atgccgcacg aaaaggccta taaggcctca
      301 cgaatggaaa agcaagcttt tgccgtacag gccatcatca tggattttgt aaaaacctat
      361 actcctcaaa aaacccttgc cgacaatacc ctttcggaac aggaaaaggt gcaactgtta
      421 ttgaacatgg cggaagaata taaattgaaa aaatggcggg gtagccgata ggttttgtaa
      481 tattgtaggg gtgactaccc tctccttatc ggagccaaag aatcggaggg cgactcaaag
      541 tcgccctccg attgcccaag gcactattgt cggggtgact gcctcttgag aaggccaaca
      601 cgcagtcacc ccgacaatat tatttaggat tttttatcgt atcggttacc accaccacat
      661 cgccggtgag ccggccgttg accatgtaca tttttttgcc gagcatatca aagtcccaac
      721 taccgtcaag gttgtccctg tcgtaaacca ggttttcaag attttttgac agatagacac
      781 ttttgccttc gggcacctcc agaatcagct ttacagactg gttgcgccat ttttcaccgg
      841 cagcaagcag aaaataatta ttgatcaata acaccgagtc ggccgctggc gtatcaaacc
      901 tgatggattc ggcacgcctg cgggcattca tcaacgtggt gcctcttgaa aattttatca
      961 cggtgagttt gtaggttccg gtcttcgagg gaatgatctt cagcttagga aatccataca
     1021 ccaccagctt atcattccca tccgaaaaaa agcagttatt aaaaacaaaa tgttcatgat
     1081 cgccgggatc actatcaaaa aaatcatcat caaaatattg ggttttattt acaagcggca
     1141 ggttgtcggc cacatctatg acaagtgttt cgcgggtttg cggcaacata acctcctgcc
     1201 tatatatgct tttttcgctg aaatttccca caatattaat tgttagaatt atagcgagta
     1261 tcagtccggt cagccataag agcgaagcgg taatggaaac aacccttatt tttgttttaa
     1321 atccggcaat catgcggatg ctgttgaaaa taagcatcag cagggggacc ccgattaata
     1381 aaatcagcga caggaacgcc atgtgttgct gccagggatt ttcaaagaaa acctggctga
     1441 aagccggaaa agacactgaa ctgattcccc atttggtaaa aaacagaaaa ctatcggggg
     1501 cggtaattga caataccagc gaaactgtca caacaattcc tataatgaga agtacaattc
     1561 cggcaaagat cagcaacgcg cgcagaaaat aataaataat ctctccaaag acactgccca
     1621 cacctgtgcc tgattccttt accgactgcg tcctgctaat ggcggcggcc atctgccgtt
     1681 gcctgttgct ataggcactt tccctcttgg cacggtgcat cctgcgtttc atatcgttgt
     1741 attctctccg cgtatgattc tttgcatcgc ctttcatctc cttgaaccgt gctttcagtt
     1801 cctcaaattc actttttatg ttttcctgta tctccgaaat agtcatgggt tcgcctttca
     1861 tattgagctt ttcctcggta gtggttgctt tggggattat tatccaaagt atcagataga
     1921 ccaaagcgct ggatccaaaa ataaacaagg caacgataaa acctattctg aaccataccg
     1981 gatccacatt gaaataatgc cccagccctg aacagacacc gcccagcact ttgttgtctt
     2041 cgtcgcgata gagccttttt ttaaacggag aacttttttc ttgtttacct tctttcgact
     2101 cttccgatga tccgatatca tcgggtttcc ccaggatttt tatggcttcc tcaacatccg
     2161 caggcacaat gattggagcg ccgttttcga ttttcacctg gaaaatctct gcgatacggc
     2221 tttcgatatc ctgaaggatt tcctctttgc cttcggtatt cgcaaaatga ttcttcagtg
     2281 aatcgagata attttcaagc aacttgtagg cttcttcatc aatctggaag aacagcccac
     2341 cgatattaat tgaaattgtt ttgttcatgg cttttatatt tattgttttt tatttttttg
     2401 ttatgagttt cactgattta acaagctctt cccagctttc atccagattt tgaagaaaaa
     2461 tgcggccggc ttcggtaagt gtataatact tacggggtgg cccagtcttc gactcttccc
     2521 accggtaatt gagaagtccg tcgttcttca ggcgtgtcat cagcggatat aaggtacctt
     2581 ctactactat cagttttccc tctttgagtt tttccatgat gtcggaagca tatacatctt
     2641 tttccgcaat gacagagagg atacaatatt ccagtattcc tttccgcatc tgtgcttttg
     2701 aattttcaat gtccatttca tacttttttg gaatccggta caaagataat acattttata
     2761 gtactatgca atacaaggta ctatttttaa aaaatattca aataacgaaa ctaaacgcct
     2821 tcaaatcggg aatatccgaa gattaatgta tatgcatttt agcacatgcg tccgtactaa
     2881 gcattatctt ttgggcttga aatctgtaat aatttattgt atatttgaat attatttcct
     2941 gtagttgaac ttcggcacac taaatcccgc ctgttttgat gcctgccgtt tttgataatc
     3001 taaaaaacaa aaaaatggat aaagaagcaa aagccgttat aatttggata acagtatgcg
     3061 tggcattcgt gatacttgcg ctggtattgt tattcgttcc atggagtatt ccccgcgaaa
     3121 tggaggtaaa atttgctttg attttaacgg gtatgctatc cttaattatc ataaccggct
     3181 tgacaaagaa aaaatacatc catgaagctg cttctgtttt aaatgtactt caatacataa
     3241 tgatagccct gctttgtgcc gttgtgttta caggatttct tttctggttt g
//
LOCUS       DAOSFP010000265         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27530_length_3265_cov_4.736760, whole genome shotgun sequence.
ACCESSION   DAOSFP010000265 DAOSFP010000000
VERSION     DAOSFP010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27530_length_3265_cov_4.736760"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2753)
                     /gene="ileS"
                     /locus_tag="PLI16_07910"
     CDS             complement(<1..2753)
                     /gene="ileS"
                     /locus_tag="PLI16_07910"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOH84522.1"
                     /translation="MKQKYTEYKQLSLTNIASEINEYWQQHDIFQKSIDTRKGCPPFV
                     FYEGPPSANGQPGIHHVMARAIKDIFCRYKTLKGYYVERKAGWDTHGLPIEIAVEKTL
                     GITKEDIGKSITVEEYNQQCRKEVMKYKDQWDDLTVKMGYWVDLENPYITFDNKYIES
                     VWHLLSKLYEKGLLYKGYTIQPYSPAAGTGLSSHELNLPGCYKDVKDNTVVAQFKVVN
                     NDHPLIKELSKKLHGELFILAWTTTPWTLPSNTALAVGSNIDYIAVQSFNQYTHQPIT
                     VILAKNLKESYFPEKNAALSMEEYLPENKNIPYRVVAEFKGKQLEEIRYEQLLPYVQP
                     DDGDAFKVVAGDFVTTEDGTGIVHIAPSFGADDFRTARQKGLGSLTLVDKQGKFVAEM
                     GEFAGRYVKNEYDPVKNNEPGNNVDIDIIIKLKKENRAFKSEKYEHSYPHCWRTDKPI
                     LYYPLDSWFIKTTACKERMIELNKTINWKPESTGTGRFGNWLENLVDWNLSRTRYWGV
                     PLPIWATEDRSEKICIGSVDQLKKEIEKAVEKGFMSTNPYKDFIDGNNSKENYNTIDL
                     HRPYVDEIVLVSPSGQKMFREPDLIDVWFDSGSMPYAQLHYPFENADIFKSKFPADFI
                     AEGVDQTRGWFFTLHAIAAMLFDSVAFKNVVSNGLVLDKNGNKMSKRLGNGVDPFETI
                     SKYGPDATRWYMITNSQPWDNLKFDSEGIGEVQRKFFGTLYNTYAFFALYANIDGFAY
                     QEEEIPVGQRPEIDRWIISELNTLIMRVDECYADYEPTRAGRLIQDFVDEYLSNWYVR
                     LCRRRFWKGDYSKDKISAYQTLYTCLKTIALLTSPIAPFFAEKLFLDLNNVTGKLQFP
                     SVHLAEFPVADNKLVDKDLEERMELAQKISSMVLSLRKKHNLRVRQPLAKIMLPITET
                     KTKV"
     gene            3001..>3265
                     /locus_tag="PLI16_07915"
     CDS             3001..>3265
                     /locus_tag="PLI16_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOH84523.1"
                     /translation="MKKLILIIIGFFLLTAPTGALAQDDTTGFVKRWLPGTITGVIRT
                     KYEQNTSNGKGRFDVRNARFGMKGNVGKYFGYKFEIDFCDEGKI"
BASE COUNT          823 a          696 c          772 g          974 t
ORIGIN      
        1 acctttgtct tggtttccgt tatgggtagc attatttttg caagcggctg acgcacacgc
       61 aaattatgtt ttttgcgtag cgataatacc atggaagata ttttctgagc gagctccatg
      121 cgttcttcaa ggtctttatc aaccaatttg ttgtcagcta ccggaaattc agcgagatgt
      181 acggacggaa actgaagttt tcccgttacg ttattgaggt cgaggaagag tttttcggca
      241 aaaaaaggag cgatgggcga tgttagtaat gcgatggttt tcaggcatgt ataaagggtc
      301 tggtaggccg aaattttgtc cttggaataa tcgcctttcc agaaacgacg ccggcataag
      361 cgcacatacc agttgctgag gtactcatcc acaaaatcct gtatcagcct gccggcacgg
      421 gttggctcgt aatcggcata gcactcgtca acccgcatta tcagtgtgtt gagttccgaa
      481 ataatccacc ggtctatctc cggacgttgt ccgacaggga tttcctcttc ctgataagca
      541 aagccgtcaa tgttggcata caaagcaaaa aacgcatagg tgttgtacag tgttccgaaa
      601 aatttccgct gcacctcacc gatgccttca ctgtcgaatt tcaggttatc ccagggttgc
      661 gaattggtta tcatgtacca gcgcgtggca tcggggccat atttggaaat ggtctcaaaa
      721 ggatctacgc cattgccgag ccgtttcgac attttgttgc cgtttttatc aagcaccaag
      781 ccgttggaaa ccacgttttt aaaggcaacg gagtcgaata acatcgccgc aatggcatgc
      841 agggtaaaaa accatcccct ggtttggtcg acgccttcgg caataaaatc ggccggaaat
      901 ttagatttaa aaatatccgc attttcaaaa ggataatgca gttgtgcata aggcatggag
      961 cccgaatcga accatacatc aatcaggtcg ggttcgcgga acatcttttg gcccgagggc
     1021 gaaaccagca caatttcatc aacataagga cggtgaaggt caatggtgtt atagttttct
     1081 tttgaattat ttccgtcgat aaaatccttg tatgggttgg tgctcataaa gcctttttca
     1141 acggcttttt cgatttcctt tttcagctga tccaccgatc ctatgcagat tttttccgag
     1201 cggtcttccg tggcccagat aggcaaagga acaccccagt atcttgtgcg tgaaaggttc
     1261 cagtccacca ggttttcgag ccagttgcca aacctcccgg taccggtgct ttcgggcttc
     1321 cagttgatgg ttttatttaa ctcaatcata cgttctttgc atgccgtggt tttgataaac
     1381 caggaatcga gcggatagta caagataggc ttgtcggtgc gccagcagtg cggataggag
     1441 tgttcgtatt tttctgactt gaaggccctg ttttcttttt tcagtttgat aatgatgtcg
     1501 atatcaacat tgttgccggg ttcgttattt ttaacagggt cgtattcgtt tttcacataa
     1561 cgtcctgcaa attcgcccat ttccgcgaca aattttccct gcttgtcaac cagggtgagc
     1621 gatcccaggc ctttttgccg ggcagtacgg aagtcgtcgg cgccaaaact gggtgcaatg
     1681 tgcacgatac ctgttccgtc ttcggttgtt acaaaatcac cggccaccac cttgaaagca
     1741 tcgccgtcgt cgggctgtac gtaagggaga agctgttcgt aacggatttc ttccagttgt
     1801 tttcctttaa attctgccac aacgcgatag ggtatgtttt tgttttccgg caggtactct
     1861 tccatcgaca gtgccgcatt tttttccggg aaatagctct ctttcaggtt tttggcaagg
     1921 ataacggtta tcggctggtg cgtgtattga ttaaaagatt gcacggcgat ataatcgatg
     1981 ttggatccaa cggccagagc cgtattggat ggcagggtcc aaggtgtggt ggtccaggca
     2041 aggatgaaca gctcgccgtg aagtttttta ctgagttctt ttatcaaagg atggtcgttg
     2101 ttaaccactt taaactgggc aacgaccgtg ttgtctttta catccttata acagccgggc
     2161 aggttgagtt cgtgcgagct tagccccgtg ccggctgccg gcgaataggg ttgtatggtg
     2221 taacccttgt atagcagacc tttttcgtat aatttgctga gcagatgcca aacggattca
     2281 atgtatttgt tgtcgaaagt aatataagga ttttcgaggt caacccaata gcccatttta
     2341 actgtgaggt cgtcccactg gtccttatat ttcatcactt ctttgcggca ttgttggtta
     2401 tattcttcta ccgtgatcga tttgccgata tcttcttttg taattcccag ggttttttca
     2461 acagctattt cgataggcaa gccgtgggtg tcccagccgg ctttgcgttc cacataataa
     2521 cccttaaggg ttttgtaacg gcaaaaaata tctttgatag cacgagccat cacatgatga
     2581 atgccgggct ggccgttggc agagggaggc ccttcgtaaa acacaaaggg cgggcacccc
     2641 ttgcgggtat caatactttt ttgaaaaata tcgtgctgct gccagtattc atttatttca
     2701 gaagcaatgt ttgtcaggct gagttgcttg tattccgtgt atttctgttt cataaagctt
     2761 aaatattgca taaaaaaacg tgcaaaggta aaaaaaaatc atgacaagcc gcctgtgaaa
     2821 tgcaaacaca gttttatcaa caaaggcgcg actttttcac aagcagtttt ttacaaaaca
     2881 ataacccagg gttaatttaa ggtcaagcca atattaagat tgcgttaatt ttttgatata
     2941 caaatgaata tatggtaaaa aacctctttt tttgcatccg caagtctcaa aattgtgaaa
     3001 atgaaaaaat taattttgat tattattgga ttctttttgt tgacagctcc aactggcgcg
     3061 ctggcgcaag acgacactac agggtttgtg aaacgctggc ttccggggac cattaccggc
     3121 gttatccgga ccaaatacga gcaaaatacc agcaacggaa agggtcgttt tgatgtgcgc
     3181 aatgcccgtt ttggcatgaa aggtaatgtg ggtaagtatt ttggttataa attcgagatt
     3241 gatttttgcg atgaaggcaa gatta
//
LOCUS       DAOSFP010000266         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27636_length_3257_cov_3.173017, whole genome shotgun sequence.
ACCESSION   DAOSFP010000266 DAOSFP010000000
VERSION     DAOSFP010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27636_length_3257_cov_3.173017"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLI16_07920"
     CDS             complement(<1..209)
                     /locus_tag="PLI16_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84524.1"
                     /translation="MNIKGCQSVFLLLALMICGCYNPSNMSSTNITANYQDESRKLNV
                     NLFANHISNDSTRIYYQFNTEGLFF"
     gene            complement(206..1579)
                     /gene="rmuC"
                     /locus_tag="PLI16_07925"
     CDS             complement(206..1579)
                     /gene="rmuC"
                     /locus_tag="PLI16_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOH84525.1"
                     /translation="MIVTIILSVAIAFIAGLGLAWLIFNPKYSRVIQEKSAMETALIK
                     KEAELSSLAGRLDDNISGLTKNLDKAVKELAEERAGKLSLTAQLSRSEEQIKTLQDKL
                     TSQKSEIEDLQNKFTDAFENLATKILTKNTLDFSATNQKNIADILTPLKEKLITFERQ
                     VDETYKNTLRDQTDLKAELKKLQDLNINISEEARNLTKALKGDTKKQGNWGEIVLERI
                     LERSGLNKGEEYETQATYRNEHGEMIRPDVVVKLPDNKHIIIDSKVSLIAYEKFVNAD
                     TADEKDRYAKLHIESLKNHIRGLSEKSYANTAGINTPDFVLMFLPIESSFSMAIQQDI
                     DLFNFAWDKKVVMVSPSTLLATLRTIESIWKHEKQTQNAIEIASEGGKLYDKFVGLIE
                     DLKKLGTQLDTVQKTYQEANKKLYSGSGNLVSKVEKLRLLGAKNNKNLPVDHKPGNFL
                     NENTEIL"
     gene            complement(1642..2880)
                     /locus_tag="PLI16_07930"
     CDS             complement(1642..2880)
                     /locus_tag="PLI16_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH84526.1"
                     /translation="MNLLIRVLVVSLVILVSELYFYRLLNILFIKNEKKKTILKFFVI
                     SFAVLFILFEGGCYLIVGAPLDDPVKYRQLFFPLSAFILVYIPKAAAALVLMIYDISR
                     YSVSFFRRKKAIGKTKKQGRTIYVAASLLMIIMLAFCIDGFVFEKTRVKIQKEDFYFS
                     QLPAGFDGFRIVQISDLHLGTFTDDAIVKEYLKTIRELSPDMLVMTGDMINVTDTELL
                     PYQEDFNAINPPYGKFAILGNHDIGDYFKMKDPPNLEAINANIIRREKETGFHVLIDS
                     ACHIYKNGDSITLIGVNNFGMFPFKKTGNLKKALSYTSPDDFKILLSHDPNHWSMEVK
                     DKTNIQMTLSGHTHAMQIAIITKFFRFSPSQFINKYWYGAYGSVNQKLYVNPGLGYSG
                     FHGRIGSRPEITVIVLHRTK"
     gene            complement(2933..>3257)
                     /locus_tag="PLI16_07935"
     CDS             complement(2933..>3257)
                     /locus_tag="PLI16_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84527.1"
                     /translation="SSWSPAYKFTTKDLSGIDQVASIGNVSIYPNPSTNGHVNVAIPM
                     TSDNTINLSVVNMVGQEIFADVLSLKAGNNLYTLNLRDKENGIYFVKLQSSDNTISRK
                     IILNK"
BASE COUNT          845 a          635 c          702 g         1075 t
ORIGIN      
        1 tgaaaaaaca agccttcggt gttgaattga taataaatcc gtgtgctgtc attgctgata
       61 tggttagcga agaggttcac attgagtttc ctcgattcat cctggtaatt ggctgtaata
      121 ttggtagagc tcatgttgga agggttatag catccgcaaa tcatcaaggc caacaaaagg
      181 aatactgatt gacaaccttt tatattcata gtatttcggt gttttcgttt aggaaattgc
      241 cgggcttatg gtccaccggc agatttttat tgttttttgc accaagcagc ctcagttttt
      301 cgactttgct gaccaggttt ccgctgccgc tataaagctt tttgttggct tcctggtatg
      361 ttttctgaac ggtatctaat tgtgttccca gtttcttaag gtcttcaatc aatcctacaa
      421 atttgtcgta cagttttccg ccttcactgg ctatttctat ggcattctgg gtttgttttt
      481 catgcttcca gatcgattct atggtgcgca gggtggcaag cagggtagaa ggactaacca
      541 taacaacttt tttgtcccag gcaaaattga acaggtctat atcctgttgt atcgccatac
      601 tgaaggatga ttcaataggc agaaacatca gcacaaagtc gggagtgtta atgccggcgg
      661 tgttggcata gcttttctcg ctcaatcccc tgatatggtt tttcagtgat tcgatatgaa
      721 gttttgcgta gcggtctttt tcatcggcag tatcggcgtt gacaaacttc tcataagcaa
      781 tcagggaaac cttagagtcg atgatgatat gtttattgtc cggaagtttt accaccacat
      841 caggccttat catttcgccg tgttcgttgc ggtaagtggc ttgtgtttcg tattcttctc
      901 ctttgttcag tcccgaacgt tccaggatgc gttccaggac tatttcgccc cagttgcctt
      961 gttttttggt gtcgcctttc agggctttgg tcaggttgcg ggcttcttcc gatatgttga
     1021 tattcagatc ctgcagtttt ttcagctcgg ctttcaggtc ggtctggtcg cgcagggtgt
     1081 ttttatacgt ttcatccacc tgcctttcga acgtgataag tttttctttc agcggggtta
     1141 ggatgtcggc aatatttttt tgattggtgg ctgaaaaatc aagggtattc ttcgtgagga
     1201 ttttggtggc gaggttttca aaggcatcgg tgaacttatt ctgtaaatct tctatttccg
     1261 atttttgcga tgttaattta tcctgaagtg tttttatttg ttcttccgag cgggatagct
     1321 gtgctgtcaa acttaatttg ccggcacgtt cttcggctag ttcttttacc gctttgtcaa
     1381 ggtttttggt aaggcccgaa atattatcgt caagacggcc tgcaaggctc gacagttctg
     1441 cttctttttt tatcaaagcg gtttccatag cggacttttc ctgtattacc ctggagtatt
     1501 ttgggttgaa aatcagccag gcaaggccaa gtccggcaat aaaagctata gctacagaaa
     1561 gtatgatggt aacaatcatc ctgaaaaaat atttactctg taaatttact gaatattttt
     1621 ttcatcacat cgtggtgggg cttattttgt gcgatgcaac actatcacgg taatttctgg
     1681 ccggctgcct attcttccgt gaaaaccgct gtatcccagg cccgggttga catataattt
     1741 ctggttaacg gatccgtaag cgccatacca gtatttattg ataaactggc tgggactgaa
     1801 acgaaagaac ttggtaataa tagctatttg catcgcatgt gtgtggcccg ataaggtcat
     1861 ttggatattt gttttatctt tgacctccat agaccaatgg ttgggatcgt gtgacagcaa
     1921 aatcttaaaa tcatccggac tggtatagga cagggctttt ttcaggttgc cggttttttt
     1981 aaagggaaac atcccgaaat tattgactcc tataagcgta atagagtcgc catttttata
     2041 aatatggcag gctgaatcaa taagcacatg aaaacctgtt tctttttctc tcctgataat
     2101 gttagcgttg atggcttcga gattgggagg atctttcatt ttgaaataat ctccaatatc
     2161 atgattgccc aatatggcaa atttgccata gggtggatta atagcattaa aatcttcctg
     2221 gtagggcaat aattcggtat cggttacatt tatcatatcg ccggtcatta ccagcatatc
     2281 cggtgaaagt tctctgatag ttttcaggta ttccttcacg atagcatcat cggtaaacgt
     2341 gcccaggtgc aaatccgaaa tttgaacgat tcggaatcca tcaaaacctg ccggtaactg
     2401 actgaaataa aaatcttctt tctgaatctt tactcgggtt ttctcaaata caaaaccatc
     2461 aatgcaaaaa gccagcatga ttatcatcaa tagcgatgcg gcgacatata tcgtgcggcc
     2521 ttgttttttt gtcttgccaa ttgctttttt ccgccggaaa aaagatacgg aataccggct
     2581 gatatcatag atcatcagca caagtgccgc ggctgctttg ggtatataaa ccagaataaa
     2641 cgcactcagg gggaaaaaaa gctgacggta tttcacagga tcatccagcg gagctccaac
     2701 aatcaaataa cacccgcctt caaacaggat aaaaagtacg gcaaaactga tgacaaaaaa
     2761 tttcaggatt gtctttttct tttcattttt tatgaaaagg atattgagca gcctgtaaaa
     2821 atataattcg gatacgagaa ttaccaggct aacaactaat actctgatta ataaattcat
     2881 aaaaaaagca gttactttaa ggtaactgct tttcaaatgt aagaaaattt tcttatttat
     2941 tcagaataat ttttcgtgaa atggtattgt cactgctttg cagtttcaca aagtagatgc
     3001 cgttttcttt atcacgcaga ttgagtgtat aaaggttgtt tccggctttt aacgagagca
     3061 catcagcgaa tatttcctga ccaaccatat ttactacact gaggttgata gtattatccg
     3121 atgtcatagg tattgccacg ttgacatggc cattggttga aggatttgga taaatgctta
     3181 catttccaat ggacgcaacc tggtcgatgc ctgagaggtc tttggtggta aatttataag
     3241 cagggctcca tgaactt
//
LOCUS       DAOSFP010000267         3243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27809_length_3243_cov_3.072146, whole genome shotgun sequence.
ACCESSION   DAOSFP010000267 DAOSFP010000000
VERSION     DAOSFP010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27809_length_3243_cov_3.072146"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="PLI16_07940"
     CDS             complement(<1..741)
                     /locus_tag="PLI16_07940"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOH84528.1"
                     /translation="MKKLFILAILIAFMGTFTAKVQAQRKCGTMEYLELQKADDPGLN
                     LRIDNYEQALQQWIEKNQDYINSTKGTVTVPVVVYIIYNTAAQNISDTRVQEQIDVLN
                     RDYAGLNTHSMQAFSSSLKVNTDLQFCLAQRTPSGAATTGIERRQTSVTSFSYSNDGA
                     KYYSTGGLDAWDPTKYMNIWVCPLGDGLCGYAQFPTSGVDATFGVVIHYEFFGVTGAT
                     ASYNLGGTTTHEIGHCFNLYHIWGDDGGT"
     gene            complement(888..1910)
                     /locus_tag="PLI16_07945"
     CDS             complement(888..1910)
                     /locus_tag="PLI16_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HOH84529.1"
                     /translation="MKNKPNPDTKRKRSFFTLSGFITLMIGLLAGFIITYLATYPSKS
                     KHKTEQITETRAGGYKYINPLIECDNYRPSETYNLAKLKKEITEYINEVKTEGKISHV
                     SVYYRDLNNGPWIGINEQANYTPASLLKVPLLITILKKAQKEKGFLASKVTYTGHLDT
                     NFRQNIGYESKLVKGQSYTIDQLLELMIVESDNDAKEMLGALISFDTVQKVIGEMGVD
                     LSGKNFNDDFLSVKEYSSFFRILYNASYLDKDMSEKALYLLSKVRFNDGLPARLPKDI
                     VIAHKFGERGYLDSPMKQLHDCGIIYLPGKPYLLCVMTKGECFDTLVNVIADISEIVY
                     SNLISQ"
     gene            complement(1918..2937)
                     /locus_tag="PLI16_07950"
     CDS             complement(1918..2937)
                     /locus_tag="PLI16_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH84530.1"
                     /translation="MKKTATATKKTQTRLFSKSRLLLFATGLIIGVASGYLTTFLIKW
                     LPRPIALQETRAGGYRFINPLLDCDNFQIADNKRLSSLKKELSNYVNNTLTTKSIKHI
                     SIYYRDLNNGPWLGINEHENLSPANVSRVPLLIALLKRAETDTGFLKKEIPFTPQINS
                     SAADKAKIADSKTLFSVGQLIELMLNNQDNEARAVLEKLMGYDYLAKVVKEMGIIAVQ
                     ENYGSKNLSVKDYTSFFRILYNASFLSREMSEKALELMSKSGTKQGIATRLPADVIVS
                     HQYGEIKLPDSPYIQLQDCGIIYSGHTPFLLCIMAKGDNIDILNNVLNDVSEIVYKNL
                     NNYRK"
BASE COUNT          922 a          596 c          663 g         1062 t
ORIGIN      
        1 cgtaccgcca tcatcgcccc aaatatggta caggttaaaa caatgtccta tttcgtgtgt
       61 ggtggtcccc cctaaattgt aagaggcagt agctcctgtc acaccaaaaa attcatagtg
      121 aattaccacg ccgaaagtag cgtcgacgcc actggttgga aattgggcat agccgcataa
      181 tccgtcgccc aatgggcaaa cccagatatt catatatttg gttggatccc aggcatccag
      241 tccgccggtt gaataatatt tggctccatc gttggaatac gaaaaggatg taacagaagt
      301 ttgccttctt tcaataccgg tagtggcggc tcctgaagga gtgcgctgtg caaggcagaa
      361 ctggaggtct gtattcactt ttaaagaaga tgaaaatgcc tgcatggagt gggtgttcaa
      421 tccggcataa tccctgttta atacatcaat ttgttcctgc accctggtat cgctgatgtt
      481 ttgtgcggca gtgttgtaaa taatataaac cactacggga actgttaccg ttccttttgt
      541 actattgata tagtcctggt ttttttcgat ccattgttgc aaggcttgtt catagttgtc
      601 gatacgtaaa ttcaaacctg ggtcgtcagc cttttgtaac tccagatatt ccatggttcc
      661 gcatttgcgc tgagcctgaa cttttgccgt gaaagttccc ataaatgcaa taagaattgc
      721 aaggataaat agttttttca tagtggtttg tttttttgta caacaaattt accttgcaaa
      781 aagtcaaaag tcaagttttt tttttgcaaa tttttcaaaa aaaatgcgat gaaatgcgat
      841 aaaacacaga taaagagggg ttaaggatag atgaaggcag ggttgtttta ttgagatatc
      901 aggttggaat aaactatctc tgaaatatct gcgatgacat ttacaagtgt gtcaaaacat
      961 tcacccttgg tcattacaca tagcaggtag ggttttccgg gaagatagat gatgccgcaa
     1021 tcatgcaatt gtttcattgg tgaatccaga tatcctcttt cgccaaattt gtgggcaata
     1081 actatatctt tgggtaaacg ggcagggagg ccatcgttaa aacgtacttt gctgagcaaa
     1141 tacagggctt tttctgacat atccttgtct aggtatgaag cattatataa tatccggaaa
     1201 aaagaggaat attcttttac tgaaagaaaa tcatcattaa agttttttcc tgacaaatcg
     1261 acacccattt ccccgataac tttttgtacc gtatcgaaac ttattagcgc cccgagcatt
     1321 tctttggcat cgttatccga ttctacaatc attaattcaa gtagttggtc tatcgtatag
     1381 gattgccctt ttaccagttt gctttcataa cctatattct ggcggaaatt tgtatccagg
     1441 tggccggtat aagtaacctt gctggctaaa aacccctttt ctttttgggc ttttttcaat
     1501 atagtaatca gcagcggtac tttcaataaa ctagcgggcg tgtaattggc ttgctcattg
     1561 attcctatcc agggaccatt attcaggtct ctgtaataga cggatacatg cgagattttg
     1621 ccctcagttt ttacttcgtt aatatactct gtgatttctt tttttaattt ggcgaggttg
     1681 tatgtttccg aaggccggta attatcgcat tcaatgaggg gattaatata cttataaccc
     1741 ccggcacggg tttctgtgat ttgttccgtt ttgtgttttg attttgatgg ataggtggct
     1801 aaataggtga tgataaaacc agccaacagg ccaatcatca gtgtgataaa cccggataag
     1861 gtgaaaaaac tgcgctttct tttggtatct ggatttggtt tgtttttcat gcgtggccta
     1921 ttttcgatag ttattcaaat ttttataaac aatttctgaa acatcattca gaacattgtt
     1981 aagaatatct atgttgtcgc cttttgccat aatacaaagc agaaatgggg tgtgtccgct
     2041 ataaatgatg ccgcagtcct gcaattgaat gtaaggcgaa tccggaagct ttatttcacc
     2101 atattggtgc gatactatga catcggcagg caacctggtg gcaatgcctt gcttggttcc
     2161 cgatttgctc attagttcaa gagctttttc cgacatttcg cggctgagaa aagatgcatt
     2221 gtataatatt ctgaaaaagg aggtgtagtc ttttaccgaa agattcttcg atccatagtt
     2281 ttcctgtacg gcaataatac ccatttcttt aacgactttt gccagatagt cataacccat
     2341 aagtttttcc aacacagctc tggcttcatt gtcctgattg tttagcatca actcaattag
     2401 ctggccaacg ctgaatagtg tttttgaatc agcaatcttt gctttgtcag cagcggagga
     2461 attgatttgc ggagtaaaag gtatctcttt ttttaaaaat ccagtgtcgg tttctgctct
     2521 ttttaataag gcaatgagca ggggaaccct tgaaacattg gcgggagata agttttcatg
     2581 ttcattaata cccagccagg gtccgttgtt taaatcccgg taataaatgg atatgtgctt
     2641 tatgcttttg gttgtcaagg tgttattaac ataattactc aactcctttt ttaatgagga
     2701 cagtcttttg ttgtcggcaa tttgaaaatt atcgcaatcc agcaggggat taataaaccg
     2761 atatcctcct gctctggttt cctggagggc gataggacgg ggtagccatt taattagaaa
     2821 agtagtaaga tatcccgaag caacaccaat tattagtcct gttgcaaaaa gcaatagacg
     2881 acttttagaa aatagcctgg tttgtgtttt ttttgttgct gttgcggtct ttttcatctg
     2941 gaaaacccgt ttaggaaatc aatcactagt taattgcaaa agaactattt tgcgataaaa
     3001 atgaaaaatt aattacatgc aaaattacga aaaaaaatga caaagtagga tattgttatt
     3061 atacaatgat agtattttat cacaaaaaat atcacgatat ttatatgata taataaatgg
     3121 ttgaaataga ctgtcagttc taatcaagag attatcattg ctacaaagca tttttatttc
     3181 aatacagcag tggtctagcc ccctattaat cggacttttt tatagacggt taaacaaagg
     3241 tac
//
LOCUS       DAOSFP010000268         3235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_27913_length_3235_cov_3.635220, whole genome shotgun sequence.
ACCESSION   DAOSFP010000268 DAOSFP010000000
VERSION     DAOSFP010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27913_length_3235_cov_3.635220"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="PLI16_07955"
     CDS             complement(<1..773)
                     /locus_tag="PLI16_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH84531.1"
                     /translation="MPDHPKKTKLPPLFIINATIGFRNFLLKTAAGMLPATYVMLEEA
                     SKVWKVKAIEIAASLNIAGHLKQQPLSVDQLAGLTGTDADALYRVLRALAGEGIFKEL
                     PGRVFKNTRLSLAIAPQKDSVKYFSMHHIGETNWALTGDMTYCVKTGKNAFEHKFNTT
                     PFEHLAVHPDENDIFNKAMTETAELSGSIMADSYPFGKYKTIVDIGGGQGLLLAQILD
                     KHPGPTGILFDQPHVVASAKEHLADNKLQNRCTITEGSF"
     gene            871..1767
                     /locus_tag="PLI16_07960"
     CDS             871..1767
                     /locus_tag="PLI16_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOH84532.1"
                     /translation="MKNKAALTYLALILAMIFWSYSYIWIKAVYHYLNPLTTVFLRLI
                     LSSVFLLLFSIFYKKLEKVQKRDVPKFLLMAFFQPFLYFLGESNGIKYVSPTVAAIMV
                     STIPLFVPFTMYFLAKEPLKSNNFLGIFISFIGVLMVVLNKDLSLAASPFGLLCMCLA
                     VSSVFGYSYLLQNMLKKYNPYTITATNNIIGIFYFMPLVLIFDFNDLVSFEFNSTLIF
                     NLLALAIFASSLAYILFNAGTKNIGVSKATVFTYLIPIFTAILSYVYLKEHFSVVKIA
                     GIFITLAGLMVGEARFYRKKKV"
     gene            complement(1901..>3235)
                     /gene="sufB"
                     /locus_tag="PLI16_07965"
     CDS             complement(1901..>3235)
                     /gene="sufB"
                     /locus_tag="PLI16_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOH84533.1"
                     /translation="IRFISKKKNEPDFMLEFRLKAYKKWLTMKQPNWAHLNIPPIDYQ
                     DIIYYAAPKKTKKAGSMDEVDPELLKTFDKLGIPLNEQKILSGVAVDAVIDSVSVKTT
                     FQKTLEEKGIIFCSISEAIQNHPELVKKHLGSVVPTGDNYFAALNSAVFTDGSFCYIP
                     KGTRCPIELSTYFRINAANTGQFERTLLIADEGAYVSYMEGCTAPRRDENQLHAAVVE
                     IIAMEDAEVKYSTVQNWYPGDKNGAGGIFNFVTKRGICKGNNSKISWTQVETGSAITW
                     KYPSCILKGDNSVGEFYSVAVTNNYQQADTGSKMIHIGKNTRSTIISKGISAGKSHNS
                     YRGLVRVNKSAVNARNFSQCDSLLMGDKCGAHTYPYIKAENKSAIIEHEATTSKISDE
                     QLFYCKQRGIDQESAINLIVNGYAKEVLNKLPMEFAVEAQKLLSISLEGSVG"
BASE COUNT          759 a          678 c          743 g         1055 t
ORIGIN      
        1 aaaaaacttc cttcggtaat ggtgcagcgg ttttgcagtt tgttgtctgc cagatgttct
       61 tttgcactgg cgacaacgtg gggctgatca aaaagaatac ccgtgggacc tgggtgttta
      121 tcgagtattt gtgccaacag caggccctgg ccgccaccaa tatcaacaat ggttttgtat
      181 tttccgaaag gatatgagtc tgccatgatg ctgcccgaaa gctcagccgt ttctgtcatg
      241 gctttattga aaatgtcatt ttcatcggga tgaacagcga ggtgctcaaa aggagtggtg
      301 ttgaatttgt gttcaaaggc atttttgcct gtctttacac aataggtcat atcacctgtc
      361 agcgcccagt tggtttcgcc gatatgatgc atggagaaat actttacgga gtctttctgc
      421 ggggctatgg ccaatgataa tcttgtgttt ttaaacactc tgccagggag ctctttaaaa
      481 atgccttctc ctgccaaagc gcgaagcacc cggtacaggg catcggcatc ggtgccggta
      541 agcccagcca gttggtcaac ggagagtggc tgttgtttaa ggtggcctgc tatatttagg
      601 ctggcagcta tctcgatggc tttgaccttc catactttcg atgcttcctc cagcatcaca
      661 taagtggccg gcagcattcc cgctgctgtt tttaacagga aattccgaaa acctatggtg
      721 gcattgataa taaaaagggg agggagtttg gtttttttgg ggtggtccgg cataattttt
      781 ttttgtaaaa ttaagataat tttcaaaaac tcttttttgg aaacctgaac cgattattgg
      841 gattaaaaca atacttttgc actcctgttt atgaagaata aggctgctct tacttacctc
      901 gcgctgatac ttgccatgat tttctggagt tactcctata tatggatcaa ggctgtgtat
      961 cactatctga atccgctcac cacggtgttt ctgcggctta tactctcgtc ggtttttctg
     1021 ctgttgtttt caatttttta taaaaagttg gaaaaagtac aaaagcgtga tgtccccaaa
     1081 ttcttgctga tggctttttt tcagcctttt ttgtatttcc tgggcgaaag caatggtatc
     1141 aaatatgttt cgcctactgt ggcagcaatt atggtttcta ctataccgtt gtttgtgcct
     1201 tttacaatgt attttctcgc caaagaaccg ttgaagtcca ataatttttt aggcattttt
     1261 atttcgttta tcggggtgtt gatggtggtg cttaataaag atctttcgct tgccgcttcc
     1321 ccttttgggc tgttgtgcat gtgcctggcg gtgagcagtg tttttggata cagctacctg
     1381 ctgcaaaaca tgctaaagaa atacaaccct tataccatca ctgcaaccaa caatattatc
     1441 gggatatttt attttatgcc actggtgctg atttttgatt tcaacgatct ggttagtttc
     1501 gaatttaatt caactttgat tttcaacctg ctggccctgg ctatttttgc ctcttcgctg
     1561 gcttatatac tttttaatgc cggcaccaaa aatattggtg tttcaaaagc tactgttttc
     1621 acttatttaa tacctatttt caccgcgata ctatcgtatg tgtatcttaa ggaacacttc
     1681 agcgtggtga agatagctgg aatattcatc accctggcag gcctgatggt aggcgaggcc
     1741 cgtttttacc ggaaaaagaa agtctgactt ataccgattg gaaaaataat atagtctaaa
     1801 cttcgcttca ctcgtttgca ctattaaata taaccttcag tataattaaa cgagaaatat
     1861 caatacgtcc tgttggacta atttatattt caatttggta ttatcccacg ctgccttcga
     1921 ggctgatgga gagcagcttt tgagcttcta cggcaaattc catcggtaat ttgttgagta
     1981 cttccttggc atagccgttg acaatgaggt tgatggcgct ttcctggtcg atgcctcgtt
     2041 gtttgcaata aaatagttgc tcgtcagaaa ttttggaggt ggtggcttcg tgctcgataa
     2101 tagctgattt gttttctgct tttatgtagg gataggtgtg ggcaccgcat ttgtcgccca
     2161 tcagcaatga gtcgcattgt gagaaattac gggcgttgac agcgctttta ttcaccctga
     2221 cgagtccgcg gtagctgtta tggctttttc cggctgaaat tcctttggag atgatagtgc
     2281 tcctggtgtt tttgccaatg tgtatcattt tgcttcccgt gtcggcttgc tgataattgt
     2341 tcgttaccgc caccgaataa aactcaccta ccgaattatc acctttcagt atgcagctgg
     2401 gatatttcca tgtaatcgcc gagcccgttt ccacctgtgt ccatgatatt tttgaattgt
     2461 tgcctttgca tattcctctt ttggtaacaa agttgaaaat gccaccggcg ccgtttttat
     2521 cgccggggta ccagttttga acggtggagt acttcacttc ggcatcttcc atggcaataa
     2581 tttccacgac cgcggcatgc agttggtttt catcgcggcg gggagcggtg cacccttcca
     2641 tataacttac gtaagcccct tcgtcggcaa tgagcagggt gcgttcaaac tgcccggtat
     2701 tggcggcatt gatgcggaaa taggtggaca gttcaatggg gcagcgcgta cccttgggaa
     2761 tataacaaaa tgagccatcg gtaaaaacag ccgaattgag cgcggcaaaa taattgtcac
     2821 ctgttggcac cacagagccc agatgttttt ttaccaattc cgggtggttc tggatagctt
     2881 cacttatgga acaaaaaatg atgccttttt cttccagagt cttctgaaag gtggtcttta
     2941 ccgaaacgct gtcgataacg gcatccacgg ccacacctga aagaatcttc tgctcattga
     3001 ggggaatgcc gagtttgtcg aaagtcttca gcaattcggg atccacttca tccatgctgc
     3061 ctgccttttt ggtttttttt ggtgcggcat agtagataat atcctggtag tcgataggcg
     3121 gtatgttgag atgtgcccag ttgggctgct tcatggtgag ccattttttg taagctttaa
     3181 gcctgaactc gagcataaaa tcgggttcgt ttttcttttt tgaaataaaa cggat
//
LOCUS       DAOSFP010000269         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28019_length_3227_cov_4.303909, whole genome shotgun sequence.
ACCESSION   DAOSFP010000269 DAOSFP010000000
VERSION     DAOSFP010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28019_length_3227_cov_4.303909"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLI16_07970"
     CDS             complement(<1..239)
                     /locus_tag="PLI16_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOH84534.1"
                     /translation="MDSTFEPINLYELNESVFRLLDKDWMLVTAGIQGNYNMMTASWG
                     GMGVLWNKQVAFIFIRPQRHTYSFAEKYPCLTLTF"
     gene            343..1104
                     /locus_tag="PLI16_07975"
     CDS             343..1104
                     /locus_tag="PLI16_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOH84535.1"
                     /translation="MQITFLGTGTSQGVPVITCTCSTCLSADPRDKRLRSSVLIESGD
                     LTLIIDTGPDFRQQMLRENVKKLDAVLITHEHKDHIGGLDDIRAYNFMQRCAMDVYAR
                     ASVQRAIKKEFNYAFSKNKYPGIPQIVQHTITNAPFEIEGLKIVPVEAKHYFLKIFGY
                     RIGTFAYLTDASDISPREKKKLLDLDVLVINALRTKKHYSHFNLEQALNLIKELQPQK
                     AYLTHISHYMGKHEETGKLLPENVQFAFDGLKISV"
     gene            1138..1770
                     /locus_tag="PLI16_07980"
     CDS             1138..1770
                     /locus_tag="PLI16_07980"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HOH84536.1"
                     /translation="MSKLWFVLFLWLFLIQYFSFGQFNPNMMNDTNRPVIPGSKPVPP
                     NLLLPKIDSVINRSITNHGLPIQPNPFSVAGDSMVVFDSLHGKLNIFVKNINKVMGTL
                     NVAVFNSYASFLNNGPVFRGAIIPVTSKNMMIPFDSVPKGIYSIAVFHDEDANGYLNK
                     NQLNIPLEGYGFSNNVLTNMGPPNYSQIKFPYSGKNKNITIYMTYFKFPK"
     gene            1876..2985
                     /locus_tag="PLI16_07985"
     CDS             1876..2985
                     /locus_tag="PLI16_07985"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOH84537.1"
                     /translation="MSQEKLTYVDFETMERFMAEVLVKSGVPEADAKICAEVLITADK
                     LGIDSHGFNRLKPIYYDRIKDGILNPVTQFEVVRESPATAVVDGHNGMGHVIAKRSMQ
                     IAIDKARMFGMGMVAVRNSTHYGIAGYYALMAAKENMIGITGTNARPSIAPTFGVENM
                     LGTNPLTFALPTDEDFPFMLDCATSVSQRGKIELYARKGMELPKGWVIDENGESKTNA
                     KEVLSDLIAEKAALTPLGGVGEETAGYKGYGYATVVEILSAALQSGSYMKMLLGIKDG
                     KKIPYPLGHFFIAIDINAFTNPEDFKHITGDILRELRASKKMPGQTRIFTAGEKEYYI
                     WLDRKDKGVPVNESLQKDIKKLVEELHITGYDFPF"
     gene            3039..>3227
                     /locus_tag="PLI16_07990"
     CDS             3039..>3227
                     /locus_tag="PLI16_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84538.1"
                     /translation="MKKVKFLMIFAGLLFLANCCFAQVNNDKPQLLTDVTVTISGMSS
                     GEITIKQLTDNELKIDKPG"
BASE COUNT          977 a          661 c          690 g          899 t
ORIGIN      
        1 aaaaaagtaa gcgtcaggca gggatacttt tctgcaaaac tgtaggtatg cctttgagga
       61 cgtataaaaa taaatgcgac ctgtttattc cataatacgc ccattccgcc ccatgaggcg
      121 gtcatcatgt tgtaattgcc ctgaatacct gcagttacca acatccagtc tttatccaac
      181 aacctgaaaa cactttcatt aagttcataa aggttgatag gttcaaatgt tgagtccata
      241 aatctcatat tttgttgata acaactaatt ttttgtaaag gtaaataaat tatatttgtg
      301 tgttgtaaaa ttgccgggaa aaacacttat accttttcac tgatgcagat aacttttctt
      361 ggaacgggca catcgcaggg ggtgccggtc attacctgta cctgcagcac ctgtttgtcg
      421 gccgatccgc gcgacaaaag gctgaggtca tcggtgctga ttgaatccgg cgatttaacc
      481 ttaatcatcg atacaggccc tgatttcaga cagcagatgc ttcgcgaaaa cgtaaaaaaa
      541 ctggatgccg tgctgataac gcatgagcat aaagatcata tcggcggact ggacgatatc
      601 agggcttaca attttatgca acgatgtgcc atggatgtat atgcccgggc atcggtacag
      661 cgggctataa aaaaagagtt caattatgcc ttcagcaaaa ataaatatcc cggcattcct
      721 cagattgtgc agcatactat taccaatgct ccatttgaaa tcgaaggcct taagatagtg
      781 cccgtggagg caaaacatta ttttttaaag atttttggct atcgtatagg tacttttgct
      841 tacctcaccg atgccagcga tatcagccca cgcgaaaaga aaaaacttct ggatttggat
      901 gtgttggtaa ttaacgcatt gcgcaccaaa aaacattatt cgcatttcaa cctcgaacag
      961 gcattaaatt tgataaagga actgcaaccc caaaaggcat atctgacaca tattagtcat
     1021 tatatgggca aacatgaaga aaccggtaaa ttattacccg aaaatgtgca atttgctttt
     1081 gacggcttaa agattagcgt ataaagtatt atttttgtaa aaaatatttt tctgttgatg
     1141 agtaagcttt ggtttgtact ttttttgtgg ttattcctga tacaatactt ttcattcgga
     1201 cagttcaatc ctaatatgat gaacgatacg aataggcctg ttattcctgg cagtaaaccc
     1261 gtccctccca atttgctgct tcccaaaatt gattcggtaa taaacaggag tattacgaac
     1321 cacgggctcc ccatacaacc caacccattt tctgttgccg gcgattcaat ggttgttttt
     1381 gattcgctgc atggcaagct gaatattttt gtgaaaaata tcaacaaagt catgggaacg
     1441 cttaatgtgg cagtttttaa cagctacgcc agttttctca ataacggccc ggtgttcagg
     1501 ggcgctatta ttcctgttac gtccaaaaat atgatgatac cgttcgattc cgtacccaaa
     1561 ggcatctatt caattgccgt ttttcacgac gaagatgcca acggttacct gaataaaaat
     1621 caattgaata ttccgttgga gggttatggc ttttccaata atgtattaac caatatgggg
     1681 cctccaaact acagccagat taaattcccg tattccggaa aaaacaaaaa tattaccatt
     1741 tatatgactt actttaaatt tccgaaatag tggatgcaaa agctatgatt ggtaagtagc
     1801 ctgagagacc tttaattaag catgttgact atttcggatt taccgttctt cgaaaaataa
     1861 aaaaaataaa taaatatgtc tcaagaaaaa ctgacttatg ttgactttga aacgatggaa
     1921 cgtttcatgg cggaagtgct tgtcaagagc ggtgtgcccg aagcggatgc caaaatttgc
     1981 gccgaggtgc ttatcacagc cgacaaactc gggattgatt cacacggttt taaccgcctg
     2041 aaacctattt attacgaccg tataaaagat ggcattctca atccggttac tcagtttgag
     2101 gttgttcgcg aaagccctgc cacggctgtt gttgatggac acaacggcat gggccacgtg
     2161 attgccaaac gttcaatgca gatagctatt gataaagcca ggatgtttgg catgggtatg
     2221 gtagccgtgc gcaattccac acattacggc atagccgggt attatgcgct gatggcagcc
     2281 aaagagaaca tgatagggat taccggtact aatgcccggc cttccatcgc gccgaccttt
     2341 ggcgtggaaa acatgctggg caccaacccg ctgacctttg ccctgcctac tgatgaagat
     2401 tttcccttta tgctcgattg cgctacatcc gtgagccagc gcggaaaaat tgaactgtat
     2461 gcccgcaaag gtatggagct ccccaaaggc tgggtaattg atgaaaatgg cgaatcaaag
     2521 accaatgcga aggaagtgct cagtgatctc atagccgaaa aagctgccct gacaccactg
     2581 ggtggagtgg gagaagaaac cgcaggatac aaaggatatg ggtatgccac ggtggtggaa
     2641 atactttcgg cagccctgca gagcggcagc tacatgaaaa tgctgctcgg gataaaagat
     2701 ggaaagaaaa ttccttaccc cttaggccat ttttttattg ccattgatat caatgcgttt
     2761 actaatccgg aagatttcaa acatattacc ggcgatatcc ttcgcgaatt gagggcttcg
     2821 aaaaaaatgc ccggacaaac gcgtattttt accgccggcg aaaaagagta ctatatttgg
     2881 ctagaccgca aagacaaagg ggttccggta aacgaatccc tgcaaaagga catcaaaaaa
     2941 ctggtagagg agctgcatat tacgggatat gattttccat tctgatgttt ttttggattt
     3001 gtattgaata ttttttaatt ttacaaaaaa ataaccgcat gaaaaaagtt aagtttttaa
     3061 tgatttttgc cggtcttttg tttctggcaa attgttgctt tgcacaggta aacaatgata
     3121 aacctcagtt gttgactgat gttactgtca caatcagcgg catgagcagt ggcgaaatca
     3181 ccattaagca attgactgac aacgaactaa aaatcgacaa accgggt
//
LOCUS       DAOSFP010000270         3211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28222_length_3211_cov_4.286439, whole genome shotgun sequence.
ACCESSION   DAOSFP010000270 DAOSFP010000000
VERSION     DAOSFP010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28222_length_3211_cov_4.286439"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2450)
                     /gene="gcvP"
                     /locus_tag="PLI16_07995"
     CDS             complement(<1..2450)
                     /gene="gcvP"
                     /locus_tag="PLI16_07995"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOH84539.1"
                     /translation="MITNNFVKRHNGPRGKEIEQMLQKIGVSSIDELINQTIPQSIRL
                     KNTLNLPEGMNEYEFLNHLKDIAGRNKIYKNFICLGYYGTITPGVITRNILENPGWYT
                     SYTPYQAEISQGRLEALLNYQTVISDLTAMPIANASLLDEGTAAAEAMIMFFNSRSRD
                     QVKNNANKFFVSKCVFPQTYEVLKTRSKPLGIELVIGNFRESNLDNTFFGALLQYPTS
                     YGKIFNYAAFVEQAHSMNVSVAVATDLMALTMLTPPGEWGADAVLGSTQRFGLPMGFG
                     GPHAGFFAVNEKFKRNIPGRIIGISVDANGSRAYRMALQTREQHIKRERATSNICTAQ
                     ALLAIMSGMYAVYHGPVGLKEIARHINILTGVLNSEIQKYGYTQENDFFFDTLKIKLP
                     DNVKITDLRNLMLENKINLRYIDEKSIGISIDETTHLNDINLLLSVFAKAAGKDFTPF
                     VCNKEACRQITTMPQELMRTSAYLEQSVFNSYHSETEMMRYIKKLEVKDLSLNRAMIP
                     LGSCTMKLNAGTEMFALSWPEFGNIHPFVPADQAEGYLYLIKELEKALCEITGFKAIS
                     FQPNSGAAGEYTGLMVIRQYHASRGQQQRDVVLIPASAHGTNPASAVMAGFKVVVVKC
                     DDHGNIDTNDLREKAVQYNETLAALMVTYPSTHGVFEENILEIVDIIHQHGGQVYMDG
                     ANMNAQVGFTSPAHIGADVCHLNLHKTFAIPHGGGGPGVGPIGVAGHLVEFLPQHVFC
                     GVLNTKNGITAVSSAPFGSAMVLTISYAYIKLMGGAGLTEATRFAILNANYLKSCLEN
                     HYPILYAGSKGRVAHEMIL"
     gene            2792..>3211
                     /locus_tag="PLI16_08000"
     CDS             2792..>3211
                     /locus_tag="PLI16_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOH84540.1"
                     /translation="MKTPLLKIDILSIYKNSLGDQVPLPSRMAHCTPDTYKAIMSISK
                     DLSEKGGKLILSDLFRSYDMQAQAYNDYASGKKQAYSPPPGGSFHEAGRAFDLDLSKI
                     KISLAKFWDLAAKYGVVPIISKPVLQSEAWHFDCRGSH"
BASE COUNT          893 a          678 c          706 g          934 t
ORIGIN      
        1 aggatcattt catgagctac gcggcctttg gagcctgcat aaagtatagg ataatggttt
       61 tcgaggcatg atttcaggta attggcgttg agtatggcaa agcgggtagc ttcggtaagg
      121 cctgcaccgc ccatcagttt tatataggca taagatattg tcagtaccat ggcgctgcca
      181 aaaggtgcgg aagaaaccgc cgtgataccg tttttggtat ttaatacacc gcagaacaca
      241 tgttgcggca aaaattcaac caggtgcccg gccacaccaa tcgggcccac ccccgggcca
      301 ccaccgccat gagggatagc aaatgtttta tggaggttga gatggcacac atcggcacca
      361 atatgagcag ggctggtaaa tccaacctgg gcattcatat tggccccatc catatacact
      421 tgtccgccat gctgatgaat gatatccaca atttcgagga tattttcttc aaaaacaccg
      481 tgtgttgaag ggtatgttac cataagtgcc gcaagagtct cgttgtactg aactgctttt
      541 tcacgcaagt cgttggtatc aatatttcca tgatcatcgc attttaccac caccacttta
      601 aaaccggcca ttaccgcgct ggcggggttg gtgccgtgag ccgaagccgg aatgagcacc
      661 acgtcccttt gttgttgtcc gcgactggca tgatattgcc tgatgaccat caggccggta
      721 tattctcctg ccgcacccga atttggctga aacgatatgg ctttaaaacc ggtaatctcg
      781 caaagagctt tttcaagttc tttgatcagg tataagtaac cttcagcctg gtcagcagga
      841 acaaacggat ggatatttcc aaattcgggc cagctaagtg caaacatctc ggtaccggca
      901 ttgagtttca tggtgcatga gcccagcgga atcatagccc ggttcagcga caaatctttt
      961 acttccagtt ttttgatgta gcgcatcatt tcggtttcgg aatgatagga attgaaaacg
     1021 gactgttcca ggtatgcgga tgtcctcatc agttcctgcg gcatggtggt tatttgccgg
     1081 caggcttcct tattgcaaac aaacggtgta aaatctttgc ctgctgcttt ggcaaaaaca
     1141 gataacagca gattgatgtc attgagatgg gtggtttcat caatggaaat acctatgctt
     1201 ttttcgtcga tatacctaag atttatctta ttttcaagca tcaggtttct caggtcagta
     1261 atcttgacgt tatcagggag ttttatcttc agggtgtcaa agaaaaaatc attttcctga
     1321 gtgtatccat atttctgaat ttcggagttc aacacgcctg tgaggatatt gatgtgccgg
     1381 gcaatttctt tgagtccaac ggggccgtga tatacggcat acatacccga catgatagca
     1441 agcaatgcct gagcggtaca gatattggaa gtggcgcgct cacgttttat gtgttgttcg
     1501 cgggtttgca gcgccattct atatgcacgg ctgccattgg catctaccga gataccaata
     1561 atacgtcccg ggatgttgcg tttaaatttt tcgttaacgg caaaaaatcc cgcatgagga
     1621 ccgccaaaac ccataggcaa tccaaaacgc tgggtagagc ccagtacggc atcagcgccc
     1681 cattcgcccg gaggggtgag catggtaagg gccatcagat cggtggctac tgcaacggac
     1741 acattcattg aatgtgcttg ttcaacaaat gccgcataat tgaaaatttt tccgtacgag
     1801 gttgggtatt gtaacaatgc accgaaaaat gtattgtcaa ggttactttc tctgaaattt
     1861 ccaataacca actctattcc caagggtttc gagcgtgttt tcaacacttc gtaggtttgt
     1921 ggaaagacac attttgaaac aaaaaatttg ttggcattat tctttacctg gtcgcggctg
     1981 cgtgagttga aaaacataat catagcttcg gcagcggctg ttccttcatc caataaagaa
     2041 gcatttgcaa tgggcatagc ggtcagatcg gaaatcacgg tttgatagtt caacagagct
     2101 tctaaacgcc cctgcgaaat ttctgcctga taaggagtat aggatgtgta ccaacccgga
     2161 ttttcaagaa tatttctcgt gataacacca ggagtgatag ttccataata gcccaaacaa
     2221 ataaaattct tgtatatttt gttacggccg gcaatatctt tcaaatgatt gagaaattcg
     2281 tattcattca tcccttcggg aagattcaaa gtatttttca gtcggatact ttgcgggatg
     2341 gtttgattga taagttcatc tattgaggaa acccctattt tttgcagcat ctgttcaatt
     2401 tctttaccac ggggcccgtt gtggcgtttt acaaaattat tagtaatcat aacttattgt
     2461 gtttacgttg tttatatttt tttagtgctt gaagtaaata ttttgacaaa gataagaaac
     2521 aggtattaat aaccggaaaa attcatacga tttttttacc cttagggccg gcaaaagaaa
     2581 aaccggcaat ccggttatga tttgtctttt caaattgtac tttttccggt gataattggg
     2641 ctaaaaccta attaattgca ttaattgtcc gataaattca aatgttttct tgcattacaa
     2701 gtttattcgt tttatttttg cttaaaaaga accatattaa cactttaata attattttta
     2761 atttttaaaa tcaataaatt aaacaaaact tatgaaaacg ccattgctaa aaatcgacat
     2821 tctttcgata tacaaaaaca gcctgggcga tcaggtacct ttgccatcgc gcatggcaca
     2881 ctgcacgccg gatacctaca aggccataat gagcataagc aaagaccttt cggaaaaagg
     2941 aggcaaattg atactaagcg atcttttccg cagttacgat atgcaagccc aggcatataa
     3001 tgattatgca tccggcaaga aacaggcata cagtcctcct ccgggtggca gttttcatga
     3061 agccggacgg gcatttgatc tggatcttag caaaataaaa atatcgctgg caaaattctg
     3121 ggatttggct gctaaatatg gtgttgttcc cattatctca aaacccgtac tccaatcgga
     3181 agcgtggcat tttgattgcc ggggcagcca t
//
LOCUS       DAOSFP010000271         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28234_length_3210_cov_4.034548, whole genome shotgun sequence.
ACCESSION   DAOSFP010000271 DAOSFP010000000
VERSION     DAOSFP010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28234_length_3210_cov_4.034548"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            318..755
                     /locus_tag="PLI16_08005"
     CDS             318..755
                     /locus_tag="PLI16_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84541.1"
                     /translation="MRIKLSILLIITFCITNISHKAEAQEKPEDLTEHFFKLYVEQSS
                     DTAIGYIFANNKWMNNYKAYVDNLKMQTKEFIAKIGQYYGYELIDKVVYSENYVLLNY
                     MLRYDRQPIKISFILYKPNQKWQIQNLKLDEKLEDDIESKQKQ"
     gene            complement(774..1283)
                     /locus_tag="PLI16_08010"
     CDS             complement(774..1283)
                     /locus_tag="PLI16_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84542.1"
                     /translation="MTNLIFRNIIRFVVLILFQVLVFNNIQVFSFVTPYVYILFVLLL
                     PFETPRWLMLVSGFALGLLIDFFSNTGGIHTAATVLIAYLRPWAQNTVSSRQEYEPGI
                     QPGIKNLGFRWFFLYSLILITIHHVFLFYLEVFRFAGFFQTLLHALLNVLYTLVFIIL
                     IQYLFYRKQ"
     gene            complement(1276..2106)
                     /gene="mreC"
                     /locus_tag="PLI16_08015"
     CDS             complement(1276..2106)
                     /gene="mreC"
                     /locus_tag="PLI16_08015"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOH84543.1"
                     /translation="MRPLLQFFLKHINFFLFLLMEFFAFFYIVKSNYYQQSVFINSAN
                     AITGGIFTMQSNLTDYLSLKIINKRLAEENARLRNNQRSAFLSTNNKTFVVDDTLYQQ
                     QYEYTEARVVANTTNKINNYITLNKGFNHGIRKEMGVITPLGVAGIVKDVSANFCTVT
                     SVLHSKSKISAKIKKNNYIGTLVWEGGDYRLANLKDIPTHVKIASGDTIVTSGYSLIF
                     PGGLMIGTVYFYDSNKDNDFYNIKIRLSTDFNKISYVYIVKSLMKPELEELKIKTQND
                     "
     gene            complement(2167..3189)
                     /locus_tag="PLI16_08020"
     CDS             complement(2167..3189)
                     /locus_tag="PLI16_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOH84544.1"
                     /translation="MGLLSLFTKEIAVDLGTANTIIIYKDKIVVEEPSIIAVNLVTGK
                     TEAVGKKAMMMHGKTHQNIRTIRPLRDGVIADFHSAEIMIKEFIKMMDNKHSLFPPSL
                     KAVISIPSGITEVEERAVRDSAEQAGAKEVRLIHEPMAAAIGIGIDVLEPNGNMIIDI
                     GGGTSEIAVIALGGIVNNKSIRIAGDEFNQDIEEYLRKQHNMNVGERTAERVKIEVGA
                     ALPEIENPPPDYAVHGRDMLTGIPREIMVNYTEIAHALDKSITKIETAVLNALDMTPP
                     ELSADIFRTGIYLAGGGALLRGLDKRLHMKTKLTVHVAEDPLKAVARGSAIALKNFDK
                     FPFLIK"
BASE COUNT          968 a          668 c          659 g          915 t
ORIGIN      
        1 ctttatgtaa aaagccgttt tgaccattta ctgcttcaaa tccatccggc acgtccagta
       61 caacattatt gacagtcgcc ttaaaaaact tgcttggggc ttctttggtc tgcgatgaac
      121 aggcaaatga agcaactata aacaacaggg ttagcattct gattttcatg tatttttatt
      181 tttttttgta cattaatttt gtattagcaa acaaatttat taattaatcc caataataag
      241 tctaattttt tcgaaaaatt gaaatatttt tacgttgaat tttaaaatac tatttttaca
      301 ccaaataacc aataactatg agaataaaac tttcaatact gttaatcata acgttctgca
      361 taacaaacat tagtcacaag gctgaagctc aggaaaaacc agaagacctt acagaacatt
      421 tttttaaact ttacgtggaa caaagctcgg atacagccat cggatacata ttcgccaata
      481 acaaatggat gaataattac aaggcttatg ttgataacct gaaaatgcag accaaggaat
      541 tcattgcgaa gataggacag tattacggat acgaactgat tgacaaagtg gtatattcag
      601 aaaactatgt cttactgaat tatatgctgc gttacgaccg gcagccaata aaaatttctt
      661 ttattctgta taagcccaac caaaaatggc agattcaaaa cctcaagctt gatgaaaaac
      721 tggaggacga tattgaatca aaacaaaagc aataatcagc aactgttttt cgtctattgc
      781 tttctgtaaa acaaatactg tatgaggatg ataaagacca gcgtgtaaag cacgtttagc
      841 agggcatgga gcaaggtctg gaaaaagccg gcaaacctga aaacttccag gtagaagaga
      901 aaaacatgat gaatagtgat caggattagt gaatacagga aaaaccaacg gaaccccaga
      961 ttttttatcc ccggctgtat gcccggttcg tattcctgcc ttgaggaaac tgtgttttga
     1021 gcccagggcc ttaaatacgc aatcaggacc gtagccgcgg tatgtatccc acccgtattt
     1081 gagaaaaaat caatcaacaa ccccaaagca aaacccgaca ccagcatcaa ccatcgtggc
     1141 gtctcgaaag gcagcagaag gacaaagaga atataaacat acggcgtaac aaatgaaaaa
     1201 acctggatat tattaaacac cagcacctga aataatatca gcacaacaaa ccgtatgata
     1261 ttccgaaaaa tcaggttagt cattttgcgt cttgattttg agttcttcga gttccggttt
     1321 catcagcgat tttacaatat atacatatga aattttgtta aaatcggtac ttagccgtat
     1381 tttgatatta taaaaatcat tgtccttgtt ggagtcgtaa aaatatacgg tgcctatcat
     1441 caatccgcct ggaaaaatca aggaatatcc gctggtaaca atggtgtcgc cgctggcgat
     1501 tttgacgtgt gtcgggatat ctttcaggtt ggccagccgg tagtctccgc cctcccacac
     1561 cagggttccg atataattgt tttttttgat tttggccgag attttcgatt tggaatgcag
     1621 cacggatgta actgtgcaga aatttgccga cacatcttta acaataccgg ctacgcccag
     1681 aggggtaatc acccccattt ccttacgtat gccatggttg aatcccttat tcagggttat
     1741 ataattgttg attttattgg tagtgttggc caccactctt gcttcggtat attcgtattg
     1801 ctgctgatac agcgtatcgt ccaccacaaa ggttttgttg ttggtcgaca ggaaagcgct
     1861 gcgctggttg ttacgcagcc gggcattttc ttcggccagt cttttgttga taattttcaa
     1921 cgacaggtaa tcggtaagat tgctttgcat ggtaaagata ccacctgtaa tggcattggc
     1981 ggaattgata aagaccgact gctggtaata attgcttttt acaatgtaaa aaaaagcaaa
     2041 aaactccatc aacagaaaca ggaagaagtt gatatgtttc agaaaaaatt gcagcagggg
     2101 gcgcataaaa aagaattaca cccgggaaca ttcccgaaga taacaatcag cgaaataaca
     2161 aaaaatctac ttaataagga acggaaattt gtcaaaattt ttcaaagcga ttgctgaacc
     2221 tctggccaca gctttcaggg ggtcttcggc cacatgcacg gtaagcttgg ttttcatgtg
     2281 cagccttttg tcgagtccgc gcaataaggc accgccgccg gcaagataaa tacctgtacg
     2341 gaaaatgtct gccgagagtt ccggaggtgt catatcgagg gcattgagaa cagcggtttc
     2401 tatcttggta atggatttat ccagggcatg agctatttcg gtataattca ccatgatctc
     2461 gcgcggaata cccgtgagca tatccctgcc gtgaactgcg tagtcagggg gcgggttttc
     2521 aatttcgggc agagcggcac caacctctat tttaacccgt tcggcagtgc gttctcccac
     2581 gttcatattg tgctgtttgc gcaggtattc ttcaatatcc tggttaaact catcacctgc
     2641 aatacggatg gatttattgt taactattcc tccgagggca ataacggcaa tttcgctggt
     2701 tccaccaccg atatcaatga tcatattgcc gttgggctcc aacacgtcga taccgatgcc
     2761 gatagctgcc gccattggct catggataag ccttacttcc ttggcgcctg cctgctcggc
     2821 ggagtcgcgc acagcacgtt cctccacctc ggtgatgcct gaaggtatac tgatcacagc
     2881 tttcaatgat ggcggaaaaa gagaatgttt gttgtccatc attttaataa attcctttat
     2941 catgatttcc gccgaatgaa aatcggcaat cacgccgtcg cgcaaaggcc tgatagtccg
     3001 tatattctgg tgtgttttac catgcatcat catggctttt tttccaacag cctcggtttt
     3061 gccggtaacg aggttcacgg caataatcga aggttcttca accacaattt tatctttgta
     3121 gattataata gtgttagcgg taccgaggtc aacggcgatt tctttagtaa ataatgacaa
     3181 caatcccata taaggtttat ttttttaatg
//
LOCUS       DAOSFP010000272         3179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28629_length_3179_cov_3.066901, whole genome shotgun sequence.
ACCESSION   DAOSFP010000272 DAOSFP010000000
VERSION     DAOSFP010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28629_length_3179_cov_3.066901"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLI16_08025"
     CDS             <1..310
                     /locus_tag="PLI16_08025"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOH84545.1"
                     /translation="KYKDTYMYRSALDTTVTDYFPEIMSKDILYENWNYYRRNFELTC
                     IVGSLFYILNIIDASVDGHLYKFDISDDLSLKVEPDMNSLSYYSGKNMAGGIKISLKF
                     "
     gene            complement(463..1887)
                     /locus_tag="PLI16_08030"
     CDS             complement(463..1887)
                     /locus_tag="PLI16_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOH84546.1"
                     /translation="MNFKMKISLLSLWVLSLPYVIFAEPVTSETAKKVVIAKIALSGY
                     GNEFSIKNITAVNNGDLIRFYAAELSPAGYMIVAADNDLPPVIAYSYINNLDAEGTFL
                     KILKNDISQRLAYKENAPAQKIKNRKMQWEALTNDTDRLLVVSQQWPPAGTTSTGGWL
                     ETNWTQSAPYNQMCPIDPVTLSRSYVGCPATAMAQILNYHKTTNNTVFDDGDDYNHNY
                     SGRTFTIDDDFSLHGFPSFPQLNAYLDTLNSHYLNNILPTNQDKAALSFACGVAATQI
                     FTSEGSGTFGVNQAMEAYSRFGCTTAQLLWSADTSLFTRLIQNMMDSLPAHLAIVDES
                     NSSGHNVVVDGYNTDNYFHVNFGWGGSSNGWYLLPDEIPYGLTVIEGVILDIMKNEYA
                     STPQIETISPLAAYPNPVSDLITIVCPQKTIIEILNIQGQILKTVYTSSAETIVSLEN
                     MASGIYVIRAISDKESVIQKFIKR"
     gene            complement(1889..>3179)
                     /locus_tag="PLI16_08035"
     CDS             complement(1889..>3179)
                     /locus_tag="PLI16_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOH84547.1"
                     /translation="ISGWDTSDYTGLAEFYNDFGNFDVEISVPGKYVVWATGNLVNPG
                     DLLNEPYLAKYREALSSSETVTIALPDDYNKGNVTKQGAWNTWKYKADNVSDFAFALS
                     DHFVWEGCSIEAGTGGRKVFIQTAYNAKHSDYKEVIAIARKTILFLSEKLPGVPYPFP
                     CMSIFDGADGMEYPMMTNVGSSEDRGDVVYAHSHEITHTYFPFYVGTNETKYGWMDES
                     MAVFLPEALQKEIEPTKDVASYTTHVYSYYGGREFEPAVMTPTYYLTPEIYFILNYGK
                     AEQILRLLEIQLGKDVFSKCLKGFMDSWKYKHPTPYDFFNCFNQISGQDLTWFWKPWY
                     FEQGFPDLTISSATYEAGKCTVEIKNSGTLPLPVYLLFDTENGEKIRYDLPADTWKDG
                     KNITIVTLEIKQKVTKITLGNSQVPDGNPSDNNFKIK"
BASE COUNT          896 a          666 c          636 g          981 t
ORIGIN      
        1 taagtacaaa gacacctata tgtacaggtc ggctttagat acaacggtta cagattattt
       61 tcccgaaatt atgtcgaaag acatactgta cgaaaactgg aattattacc gtcgaaattt
      121 tgaacttacc tgtattgtcg gcagtttgtt ttatatttta aacatcattg atgcctcggt
      181 tgacgggcat ctttataaat ttgacatctc tgatgatcta tcgttgaagg tagagccgga
      241 tatgaactcc ctctcctact acagcggaaa aaatatggcc ggcggaataa aaatctccct
      301 gaagttttag ataacttcat tttttgggaa aaaccttcag attcttttcc tttttcataa
      361 caaacgattc ccgtgatcgg ttttccctgt tgatgataaa ggcatattac gcattaaata
      421 ttatactgat tggctggtta gcaattaatg agtctgagac aattatcttt taataaattt
      481 ttgtattaca ctttctttgt cgctaatcgc tcttattaca tatatcccgc ttgccatgtt
      541 ttctaaacta actattgttt cggcagatga agtataaacg gtcttaagta tttgcccctg
      601 gatatttaga atttctataa tcgttttttg cgggcaaact atcgtaatta gatctgagac
      661 agggttcgga tatgctgcaa gtggagaaat agtttctatt tgtggcgttg aagcatactc
      721 atttttcata atatccaata ttaccccctc gataacggtg agtccgtatg gtatttcgtc
      781 agggagcagg taccatccgt tgcttgagcc tccccatccg aaattcacat gaaaataatt
      841 atccgtatta tagccatcga ccacaacatt gtgaccggaa gaatttgatt catctacgat
      901 agcaagatgt gcgggcagcg agtccatcat gttttgaata agtctggtaa aaagcgaagt
      961 gtcagccgac caaagcaatt gggcagtagt acaaccaaaa cgggaataag cttccatcgc
     1021 ctgattaaca ccgaaagttc cggaaccttc ggaagtaaaa atctgtgtgg cggccacccc
     1081 gcaggcaaac gacagggctg ccttatcctg gttggtgggc aaaatattat tcaaataatg
     1141 tgagttcagt gtgtcaagat acgcattgag ctgcggaaaa gaaggaaaac catgcaggct
     1201 gaaatcatcg tcgatggtaa acgtacggcc tgaataatta tggttataat catcaccgtc
     1261 atcgaaaacc gtgttattag ttgtcttgtg ataattgaga atttgtgcca tagctgttgc
     1321 aggacaaccg acataacttc ttgacaaagt taccggatca ataggacaca tctggttgta
     1381 aggggcgctt tgagtccaat ttgtttcaag ccagccgcct gtagaagttg tcccagcagg
     1441 tggccattgc tgcgaaacaa caagtagcct gtccgtgtca tttgttaaag cctcccattg
     1501 catttttctg tttttgattt tttgtgcagg ggcattttct ttgtatgcca ggcgttggga
     1561 aatatcattt ttcaggattt tcaaaaaagt cccttcagcg tccaaattat tgatatacga
     1621 ataggcaatt accgggggca agtcattatc ggcagccacg atcatatagc ctgcagggct
     1681 taactcagca gcataaaagc gtatgaggtc tccgttgttg acagcagtaa tgttttttat
     1741 tgaaaactca ttgccatagc ctgaaagtgc aatttttgca ataacaacct tttttgccgt
     1801 ttcgcttgtt acaggttcag caaaaatcac ataaggcaac gataataccc atagactcaa
     1861 caacgaaatt ttcattttga aattcatact attttatttt gaaattatta tccgaaggat
     1921 taccatcggg aacctgacta ttacccaatg tgatctttgt gactttctgt ttaatctcca
     1981 gtgttactat agttatgttt ttcccgtctt tccaggtgtc tgccggcaaa tcataccgta
     2041 ttttttctcc gttctcagta tcaaacaata aataaacagg caagggaagc gttcctgaat
     2101 ttttaatttc gaccgtacat tttccggctt cgtatgtagc agaagatatg gtaaggtcgg
     2161 gaaaaccctg ctcaaaatac catggcttcc agaaccacgt aaggtcctgg cctgaaatct
     2221 gattaaaaca attgaaaaag tcgtatggcg ttggatgttt gtatttccaa ctgtccataa
     2281 atcccttaag gcatttacta aagacatcct tacctaattg tatttcgagc agccttagta
     2341 tttgctccgc cttaccataa ttgagaataa aatagatttc cggtgtaagg taatacgtag
     2401 gcgtcatcac agcaggttca aattccctgc cgccgtaata tgaataaaca tgagttgtgt
     2461 atgacgccac atcttttgtg ggttcaattt ctttttgcaa tgcttcgggt aaaaaaacag
     2521 ccatgctttc gtccatccag ccatattttg tttcatttgt tcccacataa aaaggaaaat
     2581 atgtgtgtgt tatttcgtgc gaatgcgcgt aaacaacatc tcccctgtct tcggatgagc
     2641 cgacattggt catcatcggg tattccattc cgtcggcccc gtcgaaaatg ctcatgcatg
     2701 gaaagggata aggcaccccc ggcagttttt cagacagaaa taagatcgtt ttccgtgcta
     2761 tggcaataac ttctttataa tcactgtgct ttgcgttata agcagtttga ataaaaactt
     2821 ttctgccgcc cgtgccggct tcgatgctgc agccttccca aacaaaatga tcactgagtg
     2881 cgaaggcaaa atcgctgaca ttgtccgctt tatatttcca tgtattccat gctccctgtt
     2941 tggtcacatt acccttgtta taatcatcgg gaagtgcaat ggtaacggtt tcggaagaac
     3001 ttaacgcctc ccggtacttt gcaagataag gctcattcag taaatcgccg gggtttacca
     3061 ggttacctgt ggcccacaca acatatttgc ccggaacgga gatttccaca tcaaaattgc
     3121 caaaatcatt ataaaattca gccaatcctg tataatcgct ggtgtcccat ccgctgatg
//
LOCUS       DAOSFP010000273         3163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28815_length_3163_cov_4.043436, whole genome shotgun sequence.
ACCESSION   DAOSFP010000273 DAOSFP010000000
VERSION     DAOSFP010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28815_length_3163_cov_4.043436"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..794
                     /locus_tag="PLI16_08040"
     CDS             42..794
                     /locus_tag="PLI16_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOH84548.1"
                     /translation="MAKSRILIVEDEVIVSKYIESCLTNLGYEITGIYTTGEDAVANV
                     EDNNPNLILMDIRLAGKMNGTEAAVQITNMMDVPIVFLTAYTDEKTIEMAKDSDPYGY
                     LVKPFYEKELQTTIEMALHKHSKVKQLKKERDLYYSVIENKNTADSIYIRTNSRLKRV
                     KYEEICYVEALRDYITINTITENYTVRINMKEIQKYLPERDFVRIHKSFIVRMDKIFS
                     IKYTSLIVEPSMKDLPVGNFYRKDLYSRLNML"
     gene            complement(801..2957)
                     /gene="rnr"
                     /locus_tag="PLI16_08045"
     CDS             complement(801..2957)
                     /gene="rnr"
                     /locus_tag="PLI16_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOH84549.1"
                     /translation="MAKGTSRKKSGKSSSVEELVLNVFFKKPYISFNYKQISKAVGIN
                     DKAVRELIRQQLEILLKNKSLIEDKRGKYKLNPEKHPSFFPSKTYISGTVDMKQTGKA
                     YIITDEPGPDILIAPYNTNRALHGDKVRVLLLPGRQGKKPEGQVVEIVKRAKEQFVGT
                     LQVTRYYSFLIPDNQNIPVDIYIPNDKIKNAQNGDKVVVKISDWPENSKNPFGEVLQV
                     LGKPGDNNVEMQSIIAEFGFPLSFPKNVEQDAAKIDITISPEEIKKRRDFRETFTITI
                     DPEDAKDFDDAVSLCKLTNGNWEVGVHIADVSHYVKENSAIDHEAFGRGTSIYLADRV
                     VPMLPEILSNNVCSLRPNEDKLCFSAVFEMTETGQVAGEWFGKTIINSNHRFNYEEVQ
                     STIVNKTGLYAGEILTLHQLADCLREERFKSGSFDFDTEEVRFKYDENGRPIEVYIKQ
                     QQESNKLIEDFMLLANRRVAELIGRPQNNKPVKTFVYRIHDKPLPEKLDTFTKFVAKL
                     GYKINNKNTKTLADSFNALLTNVKGKGEENMINNLAIRTMAKAVYSTENIGHYGLSFR
                     FYTHFTSPIRRYPDLMVHRLLERYLANKPAVDKHEFEEKCKHSSDMEKLATDAERASI
                     KYKQAEYMADKVGQVFEGLISGVSKWGIYVEIIENKCEGMVSIRDMEDDAYFIDEENY
                     TVIGRYSNKKYRLGDKVKIKVKKILLNKKQIDFVFV"
BASE COUNT          865 a          651 c          656 g          991 t
ORIGIN      
        1 aaaattatat tatttttata gcctttaaaa attaaaaaat tatggcaaag tcaagaattt
       61 taattgtaga agatgaagta attgtttcca aatacataga atcatgttta accaacctgg
      121 gctatgagat taccggaatt tacaccacgg gggaagacgc cgtagccaat gtggaggata
      181 ataacccgaa tcttatcctt atggatatca ggctggccgg caaaatgaac ggcaccgagg
      241 ccgctgtaca gattaccaat atgatggatg tgcccatcgt ttttctgacg gcgtacaccg
      301 acgaaaaaac cattgaaatg gccaaggatt ccgatcctta tggctacctg gtaaaaccgt
      361 tttatgaaaa agaacttcag accaccatcg aaatggcttt acacaaacat tccaaagtaa
      421 agcaactgaa aaaagaacgc gacctgtatt attccgttat tgagaataaa aacacggccg
      481 acagcatcta tatccgcacc aacagccgtt tgaaaagggt taagtacgaa gaaatatgct
      541 atgtggaagc attgcgtgat tatatcacca ttaatacgat cacagaaaac tatactgtca
      601 ggatcaatat gaaagaaatt caaaaatacc tgcccgagag ggattttgta agaattcaca
      661 aatctttcat tgtgcgcatg gataaaatat tttcgataaa atataccagc ctgatagtgg
      721 aaccgtcgat gaaagatttg ccggttggta acttttatcg caaagacctt tacagcaggc
      781 tgaatatgct gtaaaacccg ttaaacaaaa acaaagtcaa tttgtttttt gtttaataaa
      841 atctttttca cttttatttt taccttatca cccaggcggt attttttgtt tgagtaacgg
      901 cctattacgg tatagttttc ctcatcaata aaataggcat cgtcttccat atcgcgtatg
      961 gacaccatgc cttcgcattt gttttcgata atctctacat aaatacccca tttagagaca
     1021 cccgaaatca agccttcaaa aacttgtcct accttgtcgg ccatatattc cgcctgttta
     1081 tattttatgg aagcacgttc ggcatcggtg gccagttttt ccatatccga tgaatgtttg
     1141 catttttcct caaactcatg cttgtccacg gcgggcttgt tggccaaata acgttccagg
     1201 agcctgtgta ccatcaaatc gggataacgg cgtatgggag aggtaaaatg cgtgtaaaag
     1261 cgaaatgaca gcccgtagtg gcctatattc tcggtggaat acaccgcctt ggccatggtg
     1321 cgtattgcca ggttgtttat catgttttct tcgcccttgc cttttacgtt tgtcaacaaa
     1381 gcattgaatg aatcagcaag ggttttggtg tttttgttgt tgattttgta tcccagctta
     1441 gccacaaatt tggtgaaggt atccagtttt tcgggcagcg gcttgtcgtg tatcctgtat
     1501 acaaaggtct ttacaggttt gttgttttgc ggtctgccaa tcaactcggc taccctgcgg
     1561 ttggccagca acatgaaatc ctcaattaat ttgttggatt cctgttgttg cttaatgtaa
     1621 acttcgatgg gccttccatt ttcatcgtat ttaaaacgta cttcttcggt atcaaaatca
     1681 aaggagccgc tcttgaaacg ttcttcccgc aagcagtcgg ccagctggtg cagggttagt
     1741 atttcaccgg cgtataagcc tgttttgttt acaattgtgg attgtacttc ttcataatta
     1801 aaacggtggt ttgagtttat gatagttttg ccgaaccact cgccggccac ctggccggtt
     1861 tcggtcattt caaataccgc cgagaagcac aatttatctt cgttgggcct gagcgaacac
     1921 acattgtttg ataaaatttc cggcagcatg ggaaccacgc ggtcggcaag gtatatagag
     1981 gtgccgcgcc cgaaagcttc gtgatcaata gccgaatttt ccttaacata atgtgatacg
     2041 tcggcgatgt gaacacctac ctcccaattg ccatttgtta atttacaaag ggaaacagcg
     2101 tcatcgaaat ctttggcatc ttccggatct atcgtaatag taaaagtttc gcgaaaatca
     2161 cggcgttttt ttatttcttc gggactaatg gtgatatcaa tttttgcggc gtcctgttct
     2221 acattttttg gaaacgaaag gggaaaccca aactcagcaa tgatggattg catttccacg
     2281 ttattatcgc ccggcttgcc caatacctgc aacacttcgc caaaaggatt cttggagttt
     2341 tcgggccagt cgctgatttt taccactacc ttgtcaccgt tttgcgcgtt ttttatttta
     2401 tcgtttggta tataaatatc aacaggaata ttttgattgt cggggatcag gaaagaataa
     2461 tagcgggtaa cctgcagcgt acctacaaac tgttctttgg ctctttttac tatttccact
     2521 acttgtcctt cgggtttttt tccctggcgt ccgggcaata gcaacaccct gactttgtcg
     2581 ccatgcagtg cgcggttggt attgtaagga gctattaaaa tatccggtcc cggctcatcg
     2641 gtgatgatat aggccttgcc tgtctgtttc atatcaacgg ttccggagat ataagtctta
     2701 ctgggaaaaa acgacgggtg tttttcggga ttcagtttgt attttcccct tttatcttcg
     2761 atgaggcttt tgtttttcag caggatttcg agttgctggc ggataagttc tcttaccgct
     2821 ttgtcgttaa tgccaaccgc ttttgaaatt tgcttgtaat taaagcttat gtagggtttt
     2881 ttaaaaaaaa cattcaggac aagttcttcg acagatgatg atttgcctga ctttttgcgg
     2941 gatgtgcctt ttgccatttt ttttcgtaaa attaaacatt attgactgac aaattactgc
     3001 tggaaccaaa gttgataaaa tgttcatgat attttaacat aaacataata tcagggttgt
     3061 gagaagatct ccttctgact aaaaagaaaa aaccaacgac gcttttttca agtgggagta
     3121 tgactgcgct ctggccatgc gcttatagca agtcatgctc cca
//
LOCUS       DAOSFP010000274         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28927_length_3154_cov_4.184253, whole genome shotgun sequence.
ACCESSION   DAOSFP010000274 DAOSFP010000000
VERSION     DAOSFP010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28927_length_3154_cov_4.184253"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /gene="rpoB"
                     /locus_tag="PLI16_08050"
     CDS             complement(<1..1026)
                     /gene="rpoB"
                     /locus_tag="PLI16_08050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOH84550.1"
                     /translation="MASNKIERINFASTKVQAEYPDFLDIQVKAFQDFFQLETTPENR
                     QNEGLYKVFAENFPISDARNNFVLEFLDYFIDPPRYGMEECLERGLTFSVPLKAKLKL
                     YCTDPDHEDFETIIQDVYLGMIPYMTPKGTFIINGAERVVVSQLHRSPGVFFGKTRHA
                     NGQPLYSARIIPFKGSWMEFATDINNVMYAYIDRKKKLPVTTLLRAIGYESDKAILEI
                     FQLADEVKVSKANLKKLVGHKLAARVVRSWIEDFVDEDTGEVVSIERTEVLIDRETVI
                     ENEHIDIIIDAGVKTILVHSETSSSTDYSIIFNTLQKDTANSEKEAVEFIYRLLRNAE
                     PPDEDTAR"
     gene            complement(1140..1523)
                     /gene="rplL"
                     /locus_tag="PLI16_08055"
     CDS             complement(1140..1523)
                     /gene="rplL"
                     /locus_tag="PLI16_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOH84551.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAKILKEEYGIEPAAAAAVVAAP
                     AAGASEGAAEAEEKSRFDVILKSAGQAKLGVVKLVKELTSLGLKEAKELVDAAPKAIK
                     EGVSKDEANALKSQLEEAGAEVEIK"
     gene            complement(1569..2096)
                     /gene="rplJ"
                     /locus_tag="PLI16_08060"
     CDS             complement(1569..2096)
                     /gene="rplJ"
                     /locus_tag="PLI16_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOH84552.1"
                     /translation="MKKEEKDKVIDDLSETLKAANNFYVTDISDLTVATTNELRRLCF
                     KRNVKLTVVKNTLLKKAMERSERNFESLYPVLKGGTSIMVCEGANVPAKLIAEFRKAN
                     KIEKPILKGAFIEESTYIGNDQLAVLVSLKSKNELIGDIIGLLQSPAKNVIGALQSGG
                     HTISGIVKTLSEKPE"
     gene            complement(2122..2814)
                     /gene="rplA"
                     /locus_tag="PLI16_08065"
     CDS             complement(2122..2814)
                     /gene="rplA"
                     /locus_tag="PLI16_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOH84553.1"
                     /translation="MAKITKKRKEALKLVDKNTTLSLEEAAVLVKKISYTKFDSSVDI
                     DVRLGVDPRKADQMVRGIVTLPHGTGKTVRVLVLCTPDKEEEAKNAGADYYGLDDYIA
                     KIKEGWTDVDVIITMPSVMPKVGALGRVLGPRGLMPNPKSGTVTMEIGKAVQEVKAGK
                     IDFKVDKYGIIHAGIAKVSFDSNKIADNAKELLQTIMRLKPSSAKGTYIKSIYMSSTM
                     SPGIQVDTKSVV"
     gene            complement(2843..>3154)
                     /locus_tag="PLI16_08070"
     CDS             complement(2843..>3154)
                     /locus_tag="PLI16_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOH84554.1"
                     /translation="RTQDSAGKLIPAIIMVYKDKSFDFIIKTPPVAVQLLEAVKKQKG
                     SAEPNRNKISSISWDQVKQIAEGKMVDLNAFTIESAMKMVAGTARSMGISIQGNPPFS
                     V"
BASE COUNT          789 a          663 c          653 g         1049 t
ORIGIN      
        1 cctggcagta tcttcatcgg ggggttcggc attacggagc aggcgataga taaattcaac
       61 agcttctttt tcggaattag cagtatcttt ttggagtgta ttgaagatta tcgaataatc
      121 tgtgctactg ctggtttcgc tgtgtaccaa tattgttttg acgcctgcgt cgatgatgat
      181 atcgatatgt tcgttttcta tgacggtttc gcggtcgata agcacttcag tacgctcaat
      241 agaaaccact tctccggtgt cttcatccac aaaatcttcg atccatgacc gtactacacg
      301 ggcagcgagt ttgtggccta caagtttttt caggttggct ttgctgactt tcacctcatc
      361 ggcaagctgg aatatttcga ggatggcctt atccgattcg taaccgatgg cacgtaacag
      421 ggtggtaacg ggcaatttct tttttcggtc gatgtaggca tacatcacat tgttaatgtc
      481 ggtggcaaac tccatccacg atcctttaaa ggggatgata cgagccgaat acaggggttg
      541 tccgttggca tgcctggttt ttccgaagaa cacaccgggc gacctgtgca actgcgacac
      601 cactaccctt tcggctccat tgatgataaa agtacctttg ggagtcatgt aagggatcat
      661 gccaaggtac acgtcctgga tgatagtttc gaaatcctca tgatcggggt ccgtgcaata
      721 gagtttgagt ttagctttta gtggcacact aaaagtcaga ccacgttcca ggcattcttc
      781 cataccatat cttggagggt cgataaaata atccagaaat tctaaaacaa aattattgcg
      841 agcatccgaa ataggaaagt tttctgcgaa aactttatat aagccctcat tttgcctgtt
      901 ttcaggcgtg gtttctaatt ggaaaaaatc ctgaaaagcc ttgacctgga tgtcaagaaa
      961 atcaggatat tctgcctgga cttttgtgga tgcgaaattg attctttcaa ttttatttga
     1021 agccaaagtc ttaaggtttt aaattaagta aaaaaagtta ttaatgaacg ctaataaaaa
     1081 agaatgttca gacacccata atttcagggt gtctgaacta ttttacaagg tatttttctt
     1141 tacttaattt caacctcagc accagcctcc tctaactgtg attttaatgc attggcttcg
     1201 tctttgctca cgccttcttt tatggcttta ggagcggcat caaccagttc tttggcttct
     1261 ttcaagccca ggctggtgag ttctttcacg agttttacca cacctaattt agcctgaccg
     1321 gctgatttga ggataacgtc gaaacgtgat ttttcttccg cttcggctgc gccttctgat
     1381 gcgccggctg cgggggctgc aacaactgct gctgctgctg cgggttcaat accatattct
     1441 tctttcagaa tttttgctaa ttcgttaact tctttaacgg ttaaattaac cagttgttct
     1501 gcaaaagctt ttaaatctgc cattttattt aatttttaat ttggtttaca ataattattt
     1561 taattagttt attcaggttt ttctgataat gtttttacga tacctgaaat cgtatgtccg
     1621 ccagactgca aagcgccgat aacatttttg gccggcgact gtaacagacc gataatatct
     1681 ccgataagct catttttgga tttgagtgac accaatacgg ctagttgatc gttgccgata
     1741 taggttgatt cctcaatgaa agcacctttt aaaataggtt tttctatttt attagccttc
     1801 ctgaattcag caatcagttt agccggcaca ttggcgcctt cgcacaccat aattgatgta
     1861 ccgcctttga gcaccggata aagagattca aaattccgtt ctgaacgttc cattgctttt
     1921 ttgagcaggg tatttttcac aacggtgagc tttacgttgc gtttgaaaca caagcgacgt
     1981 aattcgtttg tggttgcaac ggtaagatcg gaaatatccg taacgtaaaa gttattcgca
     2041 gccttaagtg tttcgctcaa gtcgtcaata accttatctt tttcttcttt tttcatatgt
     2101 ttcagattat ttccttgtta attatacgac tgattttgta tcgacctgaa taccgggact
     2161 catcgtggat gacatataga tactctttat gtatgttcct ttggctgagg atggtttcag
     2221 gcgcataatg gtttgcagca gttcttttgc gttatcggca attttgttgc tgtcgaaaga
     2281 aacttttgca ataccggcat gaatgatacc atatttatcg accttgaaat caattttacc
     2341 ggcttttacc tcttgtactg ctttaccaat ttccatggtt accgtacctg atttggggtt
     2401 aggcataagg cctctcggac caaggacacg gcctaaagca cctactttgg gcatcacgct
     2461 cggcatagta ataataacat ctacatcagt ccatccttct tttatcttgg cgatataatc
     2521 gtcgagcccg taataatcgg ctccggcgtt tttggcttct tcttccttgt cgggggtaca
     2581 aagtaccagg accctgactg ttttgccggt tccgtggggc agggtaacga taccgcgcac
     2641 catttggtcg gctttacgtg ggtcaacacc cagacgcaca tcaatatcca cagaggaatc
     2701 aaattttgta taggagattt ttttcaccaa tacagctgct tcttctaaag ataaggtagt
     2761 gtttttatca acaagcttta acgcctcttt tcttttcttt gtaattttag ccattgttcg
     2821 aatgttaaaa ggttaccttt tgttaaactg aaaagggtgg atttccctga atagatattc
     2881 ccatgctgcg tgcagtacct gccaccattt tcatggctga ttcgatggtg aatgcattca
     2941 aatccaccat tttaccctcg gcaatttgtt taacctggtc ccaactgatt gaactgattt
     3001 tattacggtt cggttcggca gagccttttt gttttttaac tgcttcgagt aattgtactg
     3061 ctacaggagg agttttaatt atgaaatcaa aggatttatc cttgtacacc atgatgatgg
     3121 caggaataag tttccctgcg ctatcctgag tacg
//
LOCUS       DAOSFP010000275         3154 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_28931_length_3154_cov_3.172314, whole genome shotgun sequence.
ACCESSION   DAOSFP010000275 DAOSFP010000000
VERSION     DAOSFP010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28931_length_3154_cov_3.172314"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLI16_08075"
     CDS             complement(<1..327)
                     /locus_tag="PLI16_08075"
                     /inference="COORDINATES: protein motif:HMM:NF017451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOH84555.1"
                     /translation="MMKLEELKVYNLSMELGEKIWSVVNTWDYYSRDTIGRQFVKAVD
                     SVAANISEGYGRYHYCENINFCYYSRGSLFEAKTWTEKSFRRGLISEKEHAEIINELV
                     VIAKMLN"
     gene            complement(371..1762)
                     /gene="lpdA"
                     /locus_tag="PLI16_08080"
     CDS             complement(371..1762)
                     /gene="lpdA"
                     /locus_tag="PLI16_08080"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOH84556.1"
                     /translation="MNFDIIVIGSGPGGYVAAIRASQLGFTVAVVEKAEVGGICLNWG
                     CIPTKALLKSASVFNYARHAADYGVKIGEDISADFEAIIRRSRGVADTMSKGIQFLFR
                     KNKIEVISGTGKVLPGKKVRVTANDGGVAVYEAAHIILATGARSRELPHLKIDGKKII
                     GYRQAMTLEKQPQSMVVIGSGAIGSEFAHFYNTLGTKVTLVEMLPNVVPVEDEEVSKQ
                     LERSFKKSGMKVYTSSIVESVDTSGELCKVTIKTPKNVELVEAEVVLSAVGVSTNIEN
                     LGLEETGIQTERGKVLVDEYYRTSQEGYYAIGDIVKGPALAHVASREGIVCVEKIAGK
                     EVLPVDYGNIPGCTYTHPEVASVGYTEKAAIEAGLSIKTGKFPFTASGKANSSGFRDG
                     FIKVIFEADSGKWLGAHFIGENVTEMIAEVVVARNMGATAHDILTAVHPHPTMSEAVM
                     EAVAAAYGEVIHI"
     gene            complement(1838..>3154)
                     /locus_tag="PLI16_08085"
     CDS             complement(1838..>3154)
                     /locus_tag="PLI16_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84557.1"
                     /translation="SDRRGNGSRYFEELGSYNHYRTHFTRLAYNRVTEKSEIHISAFF
                     KKENYIKQNETIKRNSGLYTFYNTYQNTHDEGICFTYTTRKIKNNVITAGADFKTGGC
                     SSKDIYHTSVDTIENNGKMDFYGVFVQDQWSLLKNKLVFHAALRADLVRFYDGSFHIY
                     SPTLATEFLYNYQGDYTSKLWFALSPKAGAKYMFNENYDIYTLYSWGFRPPQINDLSR
                     TGDVNKGFKLANPDLKPEHVQSIELGANIRPAKWLLMQPVFFYSVGSNFQYFVATGDS
                     VYTSGTNMKPVIKRQNVGRVNIAGFEFKTQISFTKNIHLTACYSYNHSVIGKYEMDSN
                     SVTDLTGKFLIEIPKNLVSGIFVWNNKIVNTSITAKFLDKEWVDDDNTIELDSYFVMD
                     AKLQHMFYNKINCALICQNILNKRFVDSKGYLSPGRYFLIEVSFTW"
BASE COUNT          870 a          732 c          600 g          952 t
ORIGIN      
        1 gttgagcatt tttgctatca caaccaattc attaataatt tcagcatgtt ctttttcgct
       61 aattaaacct cttctgaatg atttctctgt ccatgtttta gcctcaaata atgagcctct
      121 tgaataataa caaaagttga tattttcaca ataatggtat ctcccgtaac cttcgctgat
      181 atttgccgca accgaatcaa ccgctttgac aaattgtctt ccaatagtgt ctctagaata
      241 ataatcccac gtatttacaa cagaccatat tttttcacct aattccattg acaggttata
      301 tactttaagt tcctccaatt tcatcatttt aaaataatct taaaaatgac taatttccat
      361 tgtctaatga ctatatatga atcacttcgc cataggccgc cgccacggct tccataaccg
      421 cttctgacat ggtggggtga ggatgtaccg ccgtgagaat atcgtgagcc gtggcaccca
      481 tattacgtgc caccaccact tcggcaatca tttcggtaac gttctcgcca ataaaatgcg
      541 cgccaagcca tttgccggaa tcagcctcaa aaatcacctt gataaaacca tccctgaacc
      601 ccgaagaatt agcttttcca ctggctgtaa aagggaattt tcctgttttt atggacaacc
      661 cggcttcgat ggcagctttt tcggtatatc ctaccgaggc cacttcagga tgtgtgtagg
      721 tacatccggg aatattgccg taatcaacag gcaacacttc tttgcctgca attttctcaa
      781 cacagacaat accttcgcgc gaagctacat gagccagagc aggccccttt acgatgtcgc
      841 cgatggcata atagccttcc tggctggtcc ggtagtattc atccaccagc acttttcctc
      901 tttcggtttg tatgccggtt tcttccaagc ctaaattctc aatattggta ctcacgccga
      961 cagccgaaag aacaacttcc gcttcaacca attcaacatt tttgggagtt tttatggtaa
     1021 ctttacacaa ttctccgctg gtatcgacac tttccactat tgatgatgtg tacactttca
     1081 taccgctttt tttgaatgac cgttccagtt gtttggaaac ctcttcatct tcaaccggga
     1141 cgacattggg cagcatttcc accagagtga ctttggtgcc aagggtatta taaaaatgtg
     1201 caaattcgga accgatagcc ccggaaccga taaccaccat ggattgcggc tgtttttcca
     1261 gcgtcattgc ctggcggtat cctataattt tttttccatc aattttcaga tgcggcaatt
     1321 cgcgtgaacg cgcccctgtc gcaaggataa tatgagcagc ttcgtaaaca gccactccgc
     1381 cgtcattggc cgtaacccga acttttttgc cgggcaaaac tttgccggta ccgctgataa
     1441 cttcaatttt atttttacga aacagaaact gaattccctt actcatggta tctgccactc
     1501 ccctgctgcg ccgaataatc gcttcgaagt ccgcgctaat atcctcacca atcttcacgc
     1561 cgtaatcagc agcgtgcctt gcataattaa aaacacttgc cgacttcaac agcgctttgg
     1621 tcgggataca cccccagtta aggcaaatac cgcccacttc agctttttca acaactgcta
     1681 ctgtaaatcc taattgcgaa gccctgattg cagccacata accgcccggg ccggaaccga
     1741 taacgataat atcaaaattc atattctgtt tctttatttt ttaattgggc aaagttaaga
     1801 aaattaaaaa gcaaattctt atcaaggtga atgcttttta ccaggtaaat gaaacttcta
     1861 tcaagaagta acgccccgga gagagataac ccttgctgtc aacaaaacgc ttgttaagga
     1921 tattctgaca aataagtgcg caattgatct tattgtagaa catatgttgc agcttggcgt
     1981 ccattacaaa atacgagtca agctctatgg tattgtcatc gtccacccat tctttgtcaa
     2041 ggaatttggc tgtaatggaa gtgttcacaa ttttattgtt ccacacaaaa atgccgctaa
     2101 caagattttt gggaatctct ataagaaatt taccggtaag atcggttaca ctatttgaat
     2161 ccatttcata cttgccaata accgaatggt tataggaata acaggctgtg agatggatat
     2221 ttttcgtgaa actgatttgg gttttaaatt caaaacctgc gatattcacc ctgccaacat
     2281 tttgcctttt aataaccggt ttcatatttg ttcctgatgt gtacacagaa tcgccggtgg
     2341 caacaaaata ctgaaaatta ctgccaacac tgtagaagaa aaccggctgc ataagcaacc
     2401 attttgccgg cctgatattg gctcccaatt caatgctttg aacgtgttcg ggtttcaggt
     2461 cggggttagc cagtttgaat cctttgttta catcgcctgt tcgagaaagg tcgttgatct
     2521 gaggaggcct gaaaccccat gaatacagtg tgtaaatatc ataattttcg ttgaacatat
     2581 atttcgcccc tgctttcgga cttaatgcaa accatagttt ggaagtgtaa tcgccctgat
     2641 aattataaag aaattctgtt gccagggtag gcgaataaat atggaaacta ccgtcataaa
     2701 aacgcaccag gtcggcacga agggcggcgt gaaacactaa tttgtttttc agcaacgacc
     2761 actgatcctg aacaaaaaca ccgtaaaagt ccattttgcc gttattctcg atggtatcca
     2821 cggaggtgtg ataaatatct tttgaggaac atcccccggt tttaaaatca gcaccggccg
     2881 tgattacatt attttttatt ttccgggtgg tataagtaaa acagattcct tcgtcgtgag
     2941 tgttttggta agtattataa aaagtatata gcccggagtt tcgtttgatg gtttcgtttt
     3001 gcttgatata attttccttt ttgaaaaacg cgctgatatg aatctcggat ttttctgtca
     3061 cgcggttata ggcaaggcgt gtaaaatggg tgcggtaatg attataactg cccaactctt
     3121 cgaaataacg gctgccgttt ccccgccggt cgga
//
LOCUS       DAOSFP010000276         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29017_length_3148_cov_3.756870, whole genome shotgun sequence.
ACCESSION   DAOSFP010000276 DAOSFP010000000
VERSION     DAOSFP010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29017_length_3148_cov_3.756870"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLI16_08090"
     CDS             <1..300
                     /locus_tag="PLI16_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84558.1"
                     /translation="SLIHIRVIPHPLLEDLGIDIKKIDTYATTGKKNIPLHIGFSKKF
                     KGALELALYSSEGTELGKSLVEINQEENSAFTVNFSFSPETNFLKTEYARINSSY"
     gene            300..1316
                     /locus_tag="PLI16_08095"
     CDS             300..1316
                     /locus_tag="PLI16_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HOH84559.1"
                     /translation="MLTIDKFSVEKLFADFTGMKVLVIGDIMVDSYIWGKVERISPEA
                     PVPVVSVTKRENRLGGAANVALNIQSLGAVPLLCSISGNDQKANEFSQLLEKNHISSS
                     GILRSDKRITTTKFRIIGNNYQMLRVDEEQSHALTQPETRAFYNHIERIALAEKPHAI
                     LFEDYDKGAIDIALITWVVELAERLKIPVAVDPKKKNFSSYKNATLFKPNYKELCDGM
                     KIENIQRTPEELLKICSGFQHFHNIAKMILTLSEQGIFISSINKFGVAEGAMIPARIR
                     NVADVSGAGDTVISIATLCTALNLPLYDTAFISNIAGGLVCEEAGVVPVNKEKLLKEL
                     LNTL"
     gene            1339..2061
                     /gene="ubiE"
                     /locus_tag="PLI16_08100"
     CDS             1339..2061
                     /gene="ubiE"
                     /locus_tag="PLI16_08100"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOH84560.1"
                     /translation="MDSLLKDKKTEDIGKMFDDIAPGYDFLNHFLSFTIDKFWRAKLI
                     RLLQKENPAHILDVATGTADLALMTAKRLPQANITGIDIAEKMLEKGRKKVNKKNAAN
                     RIELCKANAENLPFADNAFDAVMVAFGVRNFQHLHTGLNEMLRVAKPGKKIFILEFSK
                     PTGVFKLIYNAYFRLYLPLAGRLISGNKKAYQYLHDSVIAFTEGDGFLDIMEQNGYCN
                     TSLRRLSGGIATIYMGEKEKII"
     gene            2114..2845
                     /locus_tag="PLI16_08105"
     CDS             2114..2845
                     /locus_tag="PLI16_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOH84561.1"
                     /translation="MKKIFRNIFCLLVFITLFDWSAVAQRRGAGQAENLPKFDRRKYH
                     FGFLLGMNYMDFAIKTVEDFSPFDSLTNATTKPDFGFDIGIVSSLRLTKYLSLRFVPT
                     LSFGNRNVEYTFRYKDSISYIQNKKIESTFMTFPLYIKYQSKRLGNFRAYIIGGAKYS
                     IDMASQAKKRNKEGEAPIKLARHDYMADIGTGVDFFLPYFKFGIELKMSYGLRDLLIK
                     ENTLYTNSVKRLNSKIFQLSFTFEG"
     gene            2913..>3148
                     /locus_tag="PLI16_08110"
     CDS             2913..>3148
                     /locus_tag="PLI16_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84562.1"
                     /translation="MRYKFLFTLAVSLCIVTNVFSQQKGETSVIDVQGGAQLTEFSVT
                     ADNYDANRHFFISEYFRENYNRANENLPVISSGIT"
BASE COUNT          954 a          739 c          644 g          811 t
ORIGIN      
        1 agcctgatac acatcagggt aattccccat cctttgcttg aagacctggg cattgacatc
       61 aaaaaaattg acacgtacgc caccaccggc aagaaaaaca ttccgctgca tattggtttt
      121 tcaaaaaaat tcaaaggagc gctggaactg gcgttatact cctccgaagg taccgagtta
      181 ggaaaaagct tagtggagat aaaccaggaa gaaaattcgg cattcactgt aaatttttcc
      241 tttagccccg aaactaattt cctgaaaaca gaatatgcac gcataaattc gagttactga
      301 tgctgactat tgataaattt tccgttgaaa aacttttcgc tgattttaca gggatgaaag
      361 tcctggtaat cggcgatatt atggttgatt cctatatatg gggcaaggta gagcgtatct
      421 cacccgaggc tcctgtacct gtcgtttcgg ttacaaagcg cgaaaaccgc ctgggcggag
      481 ccgccaacgt ggcgttgaac atacaatcgc tgggtgcggt acccctgttg tgcagcatca
      541 gcggcaatga tcaaaaagcc aatgaattca gccaactgct cgaaaaaaac catatcagca
      601 gcagcggtat cctacgcagc gataaacgca tcaccaccac caaattccgg atcatcggaa
      661 ataattacca gatgctgcgc gtggacgaag aacaatctca tgcattgaca cagcccgaaa
      721 cccgcgcgtt ctacaatcat atcgaacgta tcgccctggc cgaaaaaccc catgccatac
      781 tcttcgaaga ttacgacaaa ggcgccatag atattgccct gatcacctgg gtggtggagc
      841 tggccgaacg gctgaaaatt cccgtggctg ttgaccccaa aaagaaaaac ttctcctctt
      901 ataaaaatgc caccttgttc aagccaaact ataaagaact gtgtgatggc atgaaaatag
      961 aaaacataca gcgcacgccc gaagaactgc ttaagatatg ttcgggtttc cagcattttc
     1021 ataacatagc aaaaatgata cttaccttgt ccgaacaggg aatttttata tcgtcgataa
     1081 acaaattcgg tgtggccgaa ggcgctatga taccagccag gattagaaac gttgccgacg
     1141 tgtcgggtgc cggcgatacg gttatttcta ttgccaccct gtgtacggcc ctgaacctgc
     1201 cgctctacga cacagccttt atctcgaata tcgcgggtgg cctggtatgc gaagaagccg
     1261 gcgtggtgcc cgtcaataaa gaaaagcttt tgaaagaact tctgaatacc ctataactgc
     1321 aattttccaa attttcggat ggatagtttg ctgaaagaca aaaaaacaga ggatatcggc
     1381 aaaatgttcg acgatattgc tcccggatat gattttctga atcattttct ttcgtttact
     1441 attgataaat tctggagggc aaaacttatc cggctgttgc aaaaagaaaa ccccgcgcat
     1501 attcttgatg tggctacagg caccgccgac cttgccctga tgacagccaa acgccttccc
     1561 caggcaaata ttaccggcat cgacattgcg gaaaaaatgc tggaaaaagg cagaaaaaaa
     1621 gtcaataaga aaaacgctgc caatcgcatc gaactgtgca aggccaacgc tgaaaacctg
     1681 ccttttgccg acaacgcctt cgacgcggtt atggtagctt ttggcgtacg caattttcaa
     1741 cacctccata ccggcctaaa cgaaatgctg cgggtggcaa agcccgggaa aaagattttc
     1801 atacttgaat tttcaaaacc caccggagtt ttcaaactga tttacaatgc ttatttcagg
     1861 ctatacctgc cgcttgccgg acggttaatt tcgggcaaca aaaaggcgta ccaatacctg
     1921 cacgatagtg tgattgcttt caccgagggt gatggatttc tggatataat ggaacaaaat
     1981 ggttattgca ataccagcct ccggaggctt agcggaggca tcgccacaat ttatatgggc
     2041 gaaaaagaaa aaattatata attttgcaat actttttaat acacagcgtt atatacagtt
     2101 caaagttttt atcttgaaaa aaatattcag aaatatcttt tgcctgttgg ttttcattac
     2161 gttgttcgac tggtcagcag tagcccagag gcgcggagca ggccaggcgg aaaaccttcc
     2221 caaattcgac cgccggaaat atcactttgg atttttgctg ggtatgaact atatggattt
     2281 tgccatcaaa accgtggaag atttcagccc gtttgactcg ctcaccaatg ccaccaccaa
     2341 acccgatttt ggttttgata tcggcatagt gtccagcctg cgcctcacca aatacctgag
     2401 cctgcgcttc gtgcccacac tctcgtttgg caaccgcaat gtagaataca ctttccggta
     2461 caaagattcc atctcataca tacaaaacaa aaagatcgaa tctactttta tgacttttcc
     2521 gctatacatc aaataccagt cgaaacgtct cggaaacttt agagcctaca tcattggcgg
     2581 cgccaaatac agtatcgaca tggcctccca ggcaaaaaaa agaaataaag aaggcgaagc
     2641 ccccataaaa ctcgcccgcc acgactatat ggccgatatc ggtacgggcg ttgatttttt
     2701 tctcccttac tttaaattcg gcattgaact aaaaatgtcg tacggattgc gcgacctgct
     2761 gatcaaggaa aacacactgt acaccaacag tgtcaaacgc ctcaattcaa aaattttcca
     2821 gctttctttc actttcgaag gataaatctt cctgcttcct ttaagtttcc caagattata
     2881 ctaaatttgt agtattcaaa aaatatattc ccatgagata taaattctta tttacgctcg
     2941 ctgtttcttt atgcattgtt accaatgttt tttcacaaca aaaaggggaa acatctgtaa
     3001 ttgatgtaca gggaggcgcc cagttgaccg aattttccgt tacggcagat aactatgatg
     3061 caaacaggca ttttttcata tccgaatatt tcagagaaaa ttataaccgt gctaacgaaa
     3121 atctacctgt tataagttca ggtattac
//
LOCUS       DAOSFP010000277         3132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29230_length_3132_cov_4.011375, whole genome shotgun sequence.
ACCESSION   DAOSFP010000277 DAOSFP010000000
VERSION     DAOSFP010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29230_length_3132_cov_4.011375"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLI16_08115"
     CDS             complement(<1..618)
                     /locus_tag="PLI16_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOH84563.1"
                     /translation="MYDKVFIAGIGAVSAIGMNADETLSSLKTSRHGVCKVDFIDTIY
                     KGKIPFAEIKKSNRELKENLDLDPDTIITRTSLFGMTAAREALINAGLELSRWRCGLV
                     SATSVGGMDLSERFYDEFYADHNAGDINGVRGHDCADSTEHIASFLGIKDYLATISTA
                     CSSSANAIMFGSRLIKNNVVDVVVAGGTDSLTRFTLNGFNTLMILD"
     gene            810..1171
                     /gene="rnpB"
                     /locus_tag="PLI16_08120"
     ncRNA           810..1171
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLI16_08120"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1268..3049
                     /locus_tag="PLI16_08125"
     CDS             1268..3049
                     /locus_tag="PLI16_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOH84564.1"
                     /translation="MKLLWKYLKPQKWLIILSLTLAGISQLLNLIDPLIFGKIIDDYA
                     TQPGNRSETELVRGVLFWLGVAIVIALLARGTKSFQEYFTRLAVQKFGMQIFNDGLKQ
                     TLRLSFQEFEEQRSGETLSVLQKVRMDTQQFTNSFINILFSSIVGIGFLIWYSVTKNW
                     MLIPVFVIGILVLGSLTGLLSKKIKTTQRSINRETNKMSGIITESLRNIELVKSLGLT
                     FPEIKRLKEHTRKIFDLEMEKVKKVRTLTFFQGTMLSILKQSILFILLWLIFRKVLTT
                     GELIAMQFISTAIFGPLQDLGNIILSYREVEASVNNFDQLMAKPIERRPENPVELGDL
                     LTISFDNVVFRHKTAGSNAIDGISFKVQTGETIAFAGPSGSGKSTLVKLLLGLYKPGQ
                     GSIEFNGTPSIQIRYNELRRQIGFVSQDTQLFAGTIKDNLLFVKSDATDADISDALNK
                     AACDKLLKNSPKGINTILGEGGMKLSGGEKQRISIARAILRRPRLLIFDEATSSLDSI
                     TEEDITNTVRGFSGRPEQITILIAHRLATIMHADMIYVLEKGKIIETGTHETLLQQKG
                     LYYAMWRQQIGERRTVIPSDETPVSWS"
BASE COUNT          931 a          698 c          708 g          795 t
ORIGIN      
        1 gtccaatatc atcagggtat taaaaccgtt gagtgtgaat cgtgtcagcg agtcggtgcc
       61 gccggcaacc accacatcta ctacgttatt ttttatcagc cgggagccaa acatgatggc
      121 attggccgac gatgagcagg ctgtgctgat ggtagcgaga taatctttta ttccaagaaa
      181 cgaggcgata tgttccgtgc tgtcagcaca atcatgacca cggacgccgt tgatatcgcc
      241 ggcgttatga tctgcataaa actcatcata aaaacgttcg ctaagatcca tgccccccac
      301 cgaagtagcc gaaaccaggc cacaacgcca ccggctgagt tccaggccgg cattaatgag
      361 ggcttccctg gctgctgtca ttccgaaaag actcgtgcgc gtgattatcg tgtcgggatc
      421 caggtcaaga ttctctttca gttccctgtt tgattttttt atttcggcaa aaggtatttt
      481 acctttataa atggtatcaa taaaatctac cttgcatacg ccatgtctgg aagttttaag
      541 agaagagagt gtttcatctg cattcatacc gattgcggaa acggctccga tacctgcaat
      601 aaatacttta tcgtacatgc atattttttt tgaacagcga aattagtaat ttttagccag
      661 aaagcagtga ttttatcagg catgaaattc caggttggct aaacccgtca tccggcatag
      721 tgaacggata tataaatagc ggcattacag gccattcccc aaaaaacatt ttgcagattt
      781 ttataaaatg tgtacctttg ttcagtaaaa caacagttcg gtctgtcgct gacgccaaaa
      841 ggcggaagag gaaagtccgg gcaacaaagg gctccatact tcctaacggg aaggtctctg
      901 cacactggtg cggggaacag acagtgccac agaaaataac cgtcccgacg gtatccgtcg
      961 ggataagggt gaaaacgtga ggtaagagct cacggcttgt tatggtaaca taacatgcgg
     1021 gtaaacctta tgggttgaaa gaccaaataa accggaggtt gagggctgcc cgcccgattc
     1081 cggagggtag gtcgttagat tgctcaggcg actgcgtgac cagataaatg gcagactaaa
     1141 acagaacccg gcttacggaa ctgttgtttt actttctatt ccgtggtgtt ttataaaaaa
     1201 aacagaaaaa attatacgga taatttcttt aaaaaataca ggtacacttt ttggaagcac
     1261 caacagcatg aaattacttt ggaaatactt aaaacctcaa aaatggctga ttattctttc
     1321 attaaccctt gcgggaatca gccaactatt aaaccttatc gatccgctga tattcgggaa
     1381 aataatagat gactacgcta cgcagccagg caatcgcagc gaaacggaac tggtaagggg
     1441 cgtgttgttc tggttgggag tagctatcgt catcgcactg ctggcccggg gcaccaaatc
     1501 atttcaggag tattttaccc gcctggctgt gcagaaattc ggcatgcaga ttttcaacga
     1561 tggattaaaa cagacgctcc gcctgtcatt tcaggagttt gaagaacagc gcagcggaga
     1621 aaccttatcg gtgctgcaaa aggtacggat ggatacgcag caatttacca attcttttat
     1681 caacatattg ttttcctcca tagtgggtat cggcttttta atatggtact cggttaccaa
     1741 aaactggatg ctcatccctg tttttgtgat aggcatactc gtgttaggct ctctgaccgg
     1801 tctattaagc aaaaaaatta aaaccaccca gcgaagcatc aaccgtgaaa ccaacaagat
     1861 gtcgggaatc attaccgaat cgctgcgcaa tatcgaactg gttaaaagcc tgggactgac
     1921 attccctgaa ataaaacgcc tgaaagaaca tacccggaag atttttgatc tggaaatgga
     1981 aaaagtaaaa aaagtacgta ccctgacatt tttccagggc accatgctca gcatactaaa
     2041 acaatccatc ctgtttatcc tgctttggct gattttcaga aaagtgctga ctaccggcga
     2101 actgattgcc atgcagttta tatccacagc catcttcggg cctttacagg atttgggaaa
     2161 tattattctc agctaccgcg aagtagaagc gtcggtaaat aatttcgacc agttgatggc
     2221 aaagcccatt gagcgcaggc cggagaatcc cgtggaattg ggggatttgc ttacgataag
     2281 ttttgacaac gtggtatttc gtcataaaac agccggcagt aatgccattg acggcatctc
     2341 cttcaaggtt caaaccggcg aaaccatagc ttttgcaggt ccttccggtt caggtaagtc
     2401 cacgctggta aagctgttgt taggcctcta taaaccagga cagggtagca tagaatttaa
     2461 cggaacaccc tctatccaaa taaggtataa tgagttgcgc cgacagatag gttttgtatc
     2521 acaggacacc caattgtttg cagggaccat caaagacaat ctgctttttg tcaaatcgga
     2581 tgctacggat gccgacataa gcgatgcctt gaacaaggct gcctgcgaca aactgctgaa
     2641 aaattcgcct aaaggcatca acaccatatt aggagaagga ggtatgaaac tatcgggagg
     2701 cgaaaaacaa cgcatctcca tagcaagagc tatcctgcgc cggccgcgcc ttttgatctt
     2761 tgacgaagcc acctcctctt tggattctat aacagaagaa gatatcacca acaccgtcag
     2821 aggcttctcg ggccgcccag aacagataac catactgata gcccaccggc ttgctaccat
     2881 catgcatgcc gacatgattt acgtgctgga aaaagggaaa ataatagaaa caggtacgca
     2941 cgaaaccctt ctacaacaaa aaggcctgta ctatgccatg tggcggcagc aaatcggtga
     3001 gagacgaacg gtgattccat cagatgaaac accggtatcc tggagttaac aaagacatag
     3061 tttttatgaa gaaaaaaatt ttttctttag cacggtcaaa ttattgattt tgtaaaaata
     3121 cttttacctt tg
//
LOCUS       DAOSFP010000278         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29331_length_3125_cov_3.503583, whole genome shotgun sequence.
ACCESSION   DAOSFP010000278 DAOSFP010000000
VERSION     DAOSFP010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29331_length_3125_cov_3.503583"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="PLI16_08130"
     CDS             complement(<1..972)
                     /locus_tag="PLI16_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOH84565.1"
                     /translation="MITFVVSLTILIGGYFIYGAVLERVFGVDPQKLTPVQRLKDGVD
                     YVEIPLWRIFLIQFLNIAGLGPIFGAIAGALWGPVAFLWIVFGCIFGGMVHDYLSGML
                     SIRHDGKSIPEIVGKYMGIGVKQFMRGFTVILMIVVGAVFVKGPASILNDISGGVMHI
                     NFWIATVFIYYILATMIPIDKLIGRIYPVFGFALLFMAVGITVGIIAEGYAMPELTAA
                     SFANLHGTPDKFPIFPMMFVSIACGAISGFHSTQSPLMARCLKNEKYGKRIFAGAMAA
                     EGVVALIWAAAAMAFFGGVEGLNKEMTAHGDNAAWAVNIISNTLLGKF"
     gene            complement(1178..2647)
                     /locus_tag="PLI16_08135"
     CDS             complement(1178..2647)
                     /locus_tag="PLI16_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOH84566.1"
                     /translation="MTPLEQHFSKFKKNIVGNDLMIDTPYGKVKMIYADWIASGRFYK
                     PIEKIISEQIGPYIGNTHTETSETGTLMTLSYHLAHKKIKEHVHASDTDVIITAGFGM
                     TSVVNKLQRILGLRTCGMINKSNCLQKCEKPVVFISHMEHHSNHTSWYETNAEVVLIK
                     PGKDLLFDLNNLEKELKKYKDRKFKIGSFTACSNVTGISTPYHQIAKVMHRYGGLCFV
                     DFAASAPYCDINMHPEDPMEKLDAIFFSPHKFLGGPGSSGVLVFDSSLYPFHAPDQPG
                     GGTVDWTNPWGEYKYIDNIEAREDGGTPGFLQAIRSALCIELKNKMGTANIQQREQEL
                     LKIAFKKMPAIPGLHILAENIRHRLGVISFYIDGLHYNLAVRLLNDRFGIQVRGGCAC
                     AGTYGHYLLNVSYQQSRHITSQITRGDLSEKPGWVRLSLHPTMTDEELNYIIKAIASV
                     AKNAAKWVDDYDYNPRTNEYKPKKNMADELTRVNRWFKL"
     gene            complement(2674..>3125)
                     /locus_tag="PLI16_08140"
     CDS             complement(2674..>3125)
                     /locus_tag="PLI16_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84567.1"
                     /translation="QLSLSGKFKSIPDEMKHSLVISDINFTWNHQSRSLVSGSPIGIA
                     MINENQLNKYVEGYIEISRKRSGSILNMYFEVENDWYYFNYAQNKLQSISSNKSYNDL
                     IKKAMSSNKNILKAEDKLPQFAFIISTEKRKNDFLKKIGKSEDQNNE"
BASE COUNT          781 a          689 c          728 g          927 t
ORIGIN      
        1 aaattttcca aggagcgtat tggaaattat gttgactgcc caggcggcat tgtcgccatg
       61 tgctgtcatt tctttgttta atccctccac acctccgaaa aatgccatag cagcggctgc
      121 ccatatcagg gctaccacgc cttcggcggc catagcacct gcgaaaatac gtttaccgta
      181 tttttcattt ttcagacaac gggccatcag cggcgactgt gtgctatgaa agcccgaaat
      241 ggcgccacag gcaatagaaa caaacatcat aggaaaaata gggaacttgt cgggagtacc
      301 atgcaggttg gcaaaactgg cggcggtaag ctcaggcatg gcgtagcctt cagcgataat
      361 acccacggtg atgcccaccg ccataaataa cagcgcaaaa ccaaaaacag ggtagattct
      421 gccgattaac ttatcaatcg gtatcatggt ggccaggata taataaataa atactgtggc
      481 aatccaaaaa ttaatgtgca ttacgccccc tgagatatcg ttgaggatac tggccggccc
      541 ttttacgaaa acagcgccta ccactatcat cagtatcact gtgaaacccc tcataaactg
      601 ttttacccct atgcccatgt atttgccgac aatttcgggg atgctcttac cgtcgtggcg
      661 tatcgacaac attcccgaca ggtaatcatg aaccatgccg ccgaaaatac atccgaaaac
      721 aatccacaga aaagcaaccg ggccccacag agcgcctgct atagcgccaa agataggtcc
      781 aaggccggca atgttcagaa actgtatcag gaaaatgcgc catagtggga tttcaacata
      841 gtccacaccg tctttcaggc gctgtaccgg cgtgagcttt tggggatcaa cgccgaatac
      901 gcgttccagc acggcgccat aaataaaata tcctccgatt aatatagtga gagaaacaac
      961 aaaagtaatc ataaaaataa atttaaatat tttgtaatga taaattaatg taccttaaac
     1021 aagtattgat ttaacgtatt ccaagcctat aatccgggac aatacgtttc ttaaattaat
     1081 acttgtcagt ctctcgttgt tattcttata tgtttctttg ttgatagtat aaagcctgtt
     1141 atcctgtcgc cgtgtttccc ggtcataatc tggagtttta caatttaaac caccggttga
     1201 ctctggttaa ctcatcagcc atgttttttt tgggtttata ctcattggtc cttggattat
     1261 aatcataatc gtcaacccat tttgcagcgt ttttggccac cgacgcaatg gctttaataa
     1321 tgtaattcaa ttcttcatcg gtcatcgtgg gatgtagcga aagtcttacc catcctggtt
     1381 tttcagacag gtcgccgcgg gtgatttgag aagtgatgtg tcttgattgc tggtaactga
     1441 cattgagcag gtaatgtcca taggtgccgg cacaagcgca tccgccacgt acctgaatgc
     1501 caaaccggtc gttcagcagc ctgaccgcca ggttataatg cagtccgtcg atataaaaag
     1561 atatcactcc gagtcggtgg cggatatttt cggcaaggat atgcaggccc ggtatggccg
     1621 gcattttttt aaaggctatc ttcagcagtt cctgttctct ctgttggatg ttggccgtgc
     1681 ccattttgtt tttcagttcg atgcaaaggg cgctgcggat ggcttgcaaa aaaccgggag
     1741 tgccgccgtc ttcgcgggct tcgatattgt cgatgtattt gtattccccc caggggttgg
     1801 tccagtccac ggtaccaccg ccgggctggt cgggagcatg gaaaggataa agcgaggagt
     1861 cgaaaaccag taccccggag ctgcccggtc cccccagaaa tttatgaggc gaaaaaaaga
     1921 tggcgtcaag tttttccatg gggtcttccg ggtgcatatt gatgtcgcaa tagggcgcag
     1981 aggcagcaaa gtcaacaaaa caaagtcctc cgtaccgatg catgactttt gcaatttggt
     2041 gataaggcgt actgatgccg gtaacattgg aacaggccgt gaatgagccg attttaaatt
     2101 tgcggtcttt gtactttttc aactcctttt ccaggttgtt caggtcgaaa agcaggtctt
     2161 tgccgggttt tataagtacc acttccgcat tggtttcata ccaggaagtg tggttgctgt
     2221 gatgttccat gtgtgaaata aagaccaccg gtttttcaca tttctgcagg caattgcttt
     2281 tgttgatcat gccgcacgta cgcaacccca gtatccgctg aagcttatta accacggagg
     2341 tcatgccgaa tcctgcagtg atgattacat ctgtgtcgga ggcgtgtaca tgttctttga
     2401 tttttttatg tgccaggtgg tacgacaagg tcatcagggt gccggtttcg ctggtttcgg
     2461 tgtgggtgtt gccgatatat ggcccaatct gttcggaaat tattttctcg atgggcttat
     2521 aaaaccgtcc gctggctatc cagtcagcat agatcatttt tactttgcca taaggcgtgt
     2581 cgatcatgag gtcgttgccg acaatgtttt ttttaaattt cgaaaagtgt tgttccaggg
     2641 gtgtcatgtg ccgggttgtg gttttagcga atgttattca ttgttttgat cttcagattt
     2701 tcctattttt ttcaaaaaat cattttttct tttctctgtg gatatgatga atgcaaattg
     2761 aggcagtttg tcttctgctt tcaaaatgtt tttgttggag ctcatcgctt tttttatcag
     2821 gtcgttgtag cttttgttcg aggagatgga ttgcagtttg ttttgtgcat agttaaaata
     2881 gtaccagtcg ttttcaactt caaaatacat gttgaggatg ctcccactgc gtttgcgcga
     2941 tatttctata tagccttcca cgtacttgtt cagctggttt tcgtttatca tggctattcc
     3001 tatgggactt ccggatacca gggagcgtga ttggtggttc caggtaaaat tgatatctga
     3061 gattaccaaa ctatgcttca tttcgtccgg tatggatttg aatttccctg acaggctgag
     3121 ctggc
//
LOCUS       DAOSFP010000279         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29383_length_3121_cov_3.294521, whole genome shotgun sequence.
ACCESSION   DAOSFP010000279 DAOSFP010000000
VERSION     DAOSFP010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29383_length_3121_cov_3.294521"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..2250)
                     /locus_tag="PLI16_08145"
     CDS             complement(175..2250)
                     /locus_tag="PLI16_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOH84568.1"
                     /translation="MSDTKKFIPFVSAETNMKEFTLRAVLLGVLMAMIMGAANAYLGL
                     KAGMTIAATYTTAVLGMAVLKAFKGTILEENAARTIGSIGGNIATGAIFTLPAFFIAS
                     VWTPFYSVSHYLIAIVILASAGILGIMFTTLLRRVLVEDADLPFPESVAAAEIHKSGR
                     GGSGGSKFLLWGMGIGAFFTALVEFKFIAATWQKIVSFSKGAILLRSPGMAPAYFGVG
                     YIIGPKLGAINFSGGLLAWGLMAPVIAYFINYNDAAAVSDWAVEITRVWKAYVRYIAI
                     GGMLVGSFYTLYNMRKSLFSGIQRSFANLKKSSADSGQLLRTEKDINMRWSLIVIGII
                     MLIMFLVFHYFAGDPMAAGIATIVMLFMGFVFAAVSGYLVGIIGVSNNPTSGLTVSVL
                     IIAALLMVALGMRGQAGLAAVLGIAAFTCTSVSVAGEMMQDLKAGHILGCTPWRMQLG
                     DVFGVVAASTIMFLILTLLHLGDIKDVVGKKIEKLESENVTVVQYQGENPALKNSTYT
                     IADIKAMSPEQQNDVLAANAGFGGEKLPAPQASLMAVVAKGIVDMKMELILIIVGMFM
                     GIALILMQVKSPMLISVGMYLPIDTSFAIFLGGVMKAITDTYVKKRGYTEAQKEKINN
                     TGVLLSSGLIAGEALMGLLFAGLAFAEIKMFKMFENPSFLGSLIAMGIIGLVLIRFSL
                     GEKINNVKK"
     gene            2385..>3121
                     /locus_tag="PLI16_08150"
     CDS             2385..>3121
                     /locus_tag="PLI16_08150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH84569.1"
                     /translation="MNESEWLPSCLASIEKQTYDNLDVVICINQPENYREHPVKKAIC
                     EDNARTLELLRSYKNFPIHIIDKSSAGFGWKGKKHGVGWARKVTMDYISGIASADDII
                     LSMDADTVFNPDFCRSVVHSFHKHKKATALALPYYHRLTGDEPIDRALLRYEIYMRYY
                     AINLWRIKNPYCFTAIGSSMAVPVHAYRAIGGITPKLSGEDFYFLQKLRKYGDIIVWN
                     EEQTFPATRYSDRVFFGTGPALIKGVQ"
BASE COUNT          889 a          799 c          654 g          779 t
ORIGIN      
        1 caaaagccgc cgtatgttcg cttaatcctt cgcctttgtc gaaagaaaaa accgtaacag
       61 tacccaaagg tctcttgata atggtgcggc tcaccacagc tccaccggcg taatcgacaa
      121 tttcgggaaa ggaaaaaact ttcccacgtt ccagttcatt aggttccata aaatttattt
      181 ttttacatta ttaatttttt cgcctaagga aaatctgatc agcaccaggc caataatccc
      241 catcgctatc aggctgccca aaaaagaagg gttttcaaac attttaaaca tctttatttc
      301 ggcaaaagca aggcctgcaa acaacagtcc catcaacgct tctcccgcaa tcagtcccga
      361 tgacagaagg acaccggtgt tattaatttt ttctttttgc gcctcagtgt atcccctctt
      421 tttcacatag gtgtctgtaa tagctttcat gacaccgcct aagaaaatcg cgaaagacgt
      481 atcaatgggc agatacatgc ccacactgat gagcatgggg ctttttacct gcattaaaat
      541 cagcgctatg cccataaaca tacccacaat aatcagaata agttccattt tcatatccac
      601 aataccttta gccaccacgg ccatgaggct tgcctgcgga gcggggagtt tttcgccgcc
      661 aaaaccggca ttggccgcca aaacatcgtt ttgctgttcc ggtgacatgg cttttatgtc
      721 ggctatcgta taggttgaat tttttaatgc agggttttct ccctgatatt gcaccacagt
      781 aacgttttcg ctttcgagtt tttcaatttt tttgccgaca acatctttaa tatcgccaag
      841 gtgcagcagg gtgagaataa gaaacataat tgtcgatgcc gccaccacgc caaacacgtc
      901 gccgagctgc atacgccaag gggtacaccc gagaatatgg ccggctttca aatcctgcat
      961 catctcccct gccaccgata ccgatgtaca ggtgaatgcg gcaatgccta acacggcagc
     1021 cagtcccgcc tggccacgca tgcctaacgc caccatcaac agagctgcta tgatcagcac
     1081 cgaaacagta agtccgctgg tcggattgtt gctaacgccg ataatgccga caagatagcc
     1141 cgaaacggca gcaaacacaa atcccatgaa caacatcacg atggtggcaa taccggcagc
     1201 catcggatcg ccggcaaaat agtggaacac cagaaacatg atgagcatga tgatacctat
     1261 cactatcaac gaccagcgca tattaatatc tttttcggtg cgcaggagtt gtcccgaatc
     1321 tgcagatgat tttttcaggt tggcaaacga acgctgtatg ccgctgaaca agcttttgcg
     1381 catattatac aaggtataga acgatcctac gagcataccc ccgatagcaa tgtaccggac
     1441 ataagccttc catacacgcg ttatttcaac agcccagtcg ctgactgcgg cggcatcatt
     1501 gtaattgatg aagtaagcta ttaccggtgc catcaagccc caggcaagca gtccgccgct
     1561 gaagttaata gcgccgagtt ttggtccgat gatgtaaccc acaccaaaat acgcaggggc
     1621 cattccaggg gaacgcaaca aaatggcgcc ttttgaaaat gaaacgattt tctgccaggt
     1681 ggcggcaata aatttgaatt ctaccaaagc tgtaaagaat gcgccaatgc ccatacccca
     1741 cagcaaaaat tttgatcctc ccgatccgcc cctgcccgat ttatgtatct cggccgccgc
     1801 cacggattcg ggaaacggca agtcggcatc ctcaaccagc accctgcgca gcaaggtagt
     1861 aaacataatg cccaggatac ctgcggaagc aagtatcaca atggctataa gatagtgcga
     1921 aaccgaataa aacggtgtcc ataccgaagc aataaaaaag gcaggcaacg taaaaatggc
     1981 tcccgttgca atgttgccgc caatagagcc gatggttcgt gcggcatttt cttcaagtat
     2041 ggtgccttta aaagctttca gtacagccat tcccagcacc gccgttgtat aggttgccgc
     2101 aatggtcatg ccggccttta aacccagata ggcattagcg gcacccatga tcatagccat
     2161 caaaacaccc agcaatactg ccctcagggt aaactctttc atgttggttt cggccgaaac
     2221 aaaaggaata aatttcttgg tgtcgctcat tttccgaatt attaatttgt acaagtataa
     2281 acatttttga attattaaca gtattttttg cttatagcgg aatatttttt tcctttgccc
     2341 ttatgttgag cccaaaacca caaatttttt ttgccatacc tgtcatgaac gaatcggaat
     2401 ggctcccctc ctgccttgcc agtattgaga aacagaccta tgacaacctt gatgtggtta
     2461 tttgtattaa ccaacccgaa aattaccggg agcatcctgt aaaaaaagct atctgtgagg
     2521 ataatgcgcg tactctggaa ctcctgcgaa gctacaaaaa ctttccgata catattatcg
     2581 acaaatcatc agcaggcttt ggatggaaag gcaaaaaaca tggcgtaggc tgggcgcgca
     2641 aagttaccat ggattatatc tcgggcatag catcggcaga cgatattatc ctgagcatgg
     2701 atgccgacac cgtgttcaac cctgactttt gccggtcggt ggttcatagt tttcacaaac
     2761 ataaaaaagc cacagccctt gccctaccct attatcacag gcttacaggc gacgagccca
     2821 tcgaccgtgc gctgcttcga tacgaaatat atatgcgcta ttacgccatc aacctttggc
     2881 ggataaaaaa cccatactgt ttcacggcga tcggatcgtc gatggctgtt ccggtgcatg
     2941 cctaccgtgc cattggaggc attacgccaa aactcagcgg tgaagatttt tattttctgc
     3001 aaaaattaag aaagtacggc gacattattg tatggaacga agagcaaact tttcctgcaa
     3061 ccaggtattc cgacagggtg tttttcggca ccggccctgc ccttattaaa ggtgtacaaa
     3121 a
//
LOCUS       DAOSFP010000280         3113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29491_length_3113_cov_3.179856, whole genome shotgun sequence.
ACCESSION   DAOSFP010000280 DAOSFP010000000
VERSION     DAOSFP010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29491_length_3113_cov_3.179856"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1642)
                     /locus_tag="PLI16_08155"
     CDS             complement(83..1642)
                     /locus_tag="PLI16_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOH84570.1"
                     /translation="MFEEKMFSEKDQQQILEKGILMEQITRQIENFRTGFPFATLVKP
                     ATTASGLMVFDEKQAKKFAAYFTENMKFISVIKFVPASGAASRMFSHLFAWHEKNRNS
                     ENQPLSDSHYDSNSTEYFNRNIRNLAFFDDLKEAMTAGGHDIEACIKDQSYVPVLDFL
                     LTEKGLNYAALPKALIKFHRYPGRNRTALDEHLAEAAELCRDIDQADIHFTISPEHIK
                     KFESHLQRVLPYFEDKFKIKYNITHSEQKPSNDIIAVDENNQPFRNPDGSLLFRPGGH
                     GALIHNLNELDADVVFVKNIDNVAPHSECGDKVFYKKVLAGYLLYIRYHVYEYLRVLD
                     NDNVSDDLIVRITGFVQKELLHDLPEKFHSLEPAGKVDYLKKILNRPIRVCGMVKNQG
                     EPGGGPFFVKDAQGNISQQIVESSQVNMNEEQQKSIFKQSTHFNPVDLVCSLRDYKGN
                     KFDLLQFVDPATGFISSKTKDGKPLRAQELPGLWNGAMAFWNTIFVEVPISTFNPVKT
                     VNDLLRKEHLE"
     gene            1744..2598
                     /locus_tag="PLI16_08160"
     CDS             1744..2598
                     /locus_tag="PLI16_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOH84571.1"
                     /translation="MKEKFYEWIEKLIPWLLNHGIKILFISLAMFILYKLVGRAIKKI
                     IRLSVKADKDSSPEAQKKRELTLLRIFNWVSRVLFFLLGGLMILQEAGIPVGPLLAGA
                     GIVGIAVGFGGQYLIRDLLSGFFIIFENQYRIGDVVSFDKTSGVVEDISLRLTTLRDM
                     DGTVHHVPHGEIKIVSNLSKDYARINMDIGVAYNTSFDHVIKVINEVGYELSNDPQWK
                     ELIINPPQFLRVAEFAESAIIIKILGETQPMKQWEVSGELRKRLKIAFDKEGIEIPFP
                     QRVMHQAV"
     gene            2713..>3113
                     /locus_tag="PLI16_08165"
     CDS             2713..>3113
                     /locus_tag="PLI16_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84572.1"
                     /translation="MKFIRVTLVFLFIVTLFTACKKEGPQGPAGPEGPAGSTTAVNPA
                     IYGKWQVTSGMPDTKYIILKSNNDIYRLDSADYGFKSLTSCMALLTYTQINAFLITYN
                     YTITNNTLTLSNLNGTIMLTKNDNAPDETQWV"
BASE COUNT          895 a          630 c          640 g          948 t
ORIGIN      
        1 aatcgttttg gtgcattagt attgcttgtt ccgcctgtgg cggattggtt ttttttagaa
       61 tttaaaatac tttgtatgtt ttctactcca ggtgttcctt cctcaacaag tcattcaccg
      121 ttttcacagg gttgaaggta ctgatgggaa cttctacaaa aatagtgttc cagaaagcca
      181 tggcgccgtt ccacaaaccg gggagttctt gtgcccttaa cggtttcccg tccttggtct
      241 ttgaagaaat aaagccggta gcaggatcga caaactgcag cagatcaaat ttgtttcctt
      301 tgtaatccct gagactgcat accagatcaa ccggattaaa atgagtggac tgtttaaata
      361 tgcttttctg ctgctcctca ttcatgttta cctgtgaact ttccacgatc tgctggctga
      421 tatttccctg cgcatctttc acaaagaacg gccctccgcc gggctcgccc tggtttttta
      481 ccatgccgca cacacggatg ggccggttga gaattttttt caaataatct accttgcctg
      541 caggttcaag tgaatggaat ttttcgggca aatcatgtaa aagctctttt tgaacaaatc
      601 cggttatcct cactatcagg tcatccgaaa cattatcatt atccaaaacc cgcaggtatt
      661 catacacatg gtatctgata tacaacaggt acccggccag cactttctta taaaacacct
      721 tgtcgccaca ttcactatgc ggtgcgacat tgtcaatatt ttttacaaaa accacatcgg
      781 catcaagctc attcaggttg tgtatcagcg cgccgtgccc gcccgggcgg aacagcaggc
      841 tgccgtcggg attgcgaaaa ggctgattgt tctcatcaac agcaatgata tcgttagaag
      901 gtttttgctc ggaatgcgtg atattgtatt ttattttgaa tttatcttca aaatatggca
      961 atacacgctg aagatgggat tcaaattttt tgatgtgttc cggtgaaatg gtgaaatgga
     1021 tatcggcctg atcaatgtcg cggcataatt cagccgcttc ggccaaatgc tcatccaaag
     1081 ccgtacggtt tctgcccggg taacgatgaa attttatcaa tgccttgggc aaagcggcat
     1141 aattaagccc tttttcggtg agaagaaaat caaggacagg gacgtagctc tgatctttga
     1201 tacatgcctc aatatcatgt ccgcctgccg tcattgcttc tttgagatca tcgaaaaacg
     1261 ccaaattccg gatgttccgg ttaaaatatt ccgtggagtt actatcataa tgggaatcag
     1321 aaagcggctg attttcggaa tttctgtttt tctcatgcca ggcaaacaaa tggctgaaca
     1381 tacgtgaggc agcgccggaa gccgggacaa attttatcac actgataaac ttcatgtttt
     1441 cagtaaaata agcggcaaat tttttggctt gtttctcatc aaaaaccatt agtcctgatg
     1501 cagtggtagc aggttttaca agtgttgcaa aaggaaatcc tgtcctgaag ttttctattt
     1561 gtcgcgtaat ctgctccatc agaataccct tttctaaaat ctgttgctgg tctttttcgg
     1621 aaaacatttt ttcttcaaac atacaattaa ttttttaaaa aaaagatgac gttttttttg
     1681 atatatacat aaaatatggt ttgatttttt aaccctatat ttgcaaaaaa taagtgcaaa
     1741 tttatgaaag aaaaatttta tgaatggatt gaaaagttaa ttccctggtt attaaatcat
     1801 ggcataaaga tacttttcat cagcctggcg atgttcattt tatacaagct ggttgggagg
     1861 gcgataaaaa aaatcatacg cctcagcgta aaagccgaca aagatagttc gcccgaggcc
     1921 cagaaaaaaa gggaattgac actgctcaga atattcaatt gggtcagccg ggtattattc
     1981 tttttgcttg gagggcttat gatattacag gaagccggca ttcccgtggg gccgcttttg
     2041 gcaggcgccg gtattgtagg cattgccgtt ggttttggag gacaatatct tatccgcgat
     2101 ttgctttcgg gtttttttat aatatttgaa aatcaataca ggataggcga tgtggtcagt
     2161 tttgacaaaa catcgggtgt tgtggaagat atcagtttgc gccttacaac cttacgcgat
     2221 atggacggca ccgtgcatca tgttccgcac ggtgaaataa aaattgtttc caacctgtca
     2281 aaagattatg cacgtatcaa tatggatatc ggtgtggcct acaatacctc ctttgatcat
     2341 gtgattaagg ttattaatga agtcgggtat gaattatcaa acgaccctca gtggaaagaa
     2401 ctcatcatca atccgcccca atttctaaga gtggctgaat ttgccgaatc agcaatcata
     2461 ataaaaatac ttggcgaaac acaacctatg aaacaatggg aagttagcgg cgaactccgc
     2521 aagcggctta aaattgcctt tgacaaagaa ggtattgaga ttccatttcc tcagcgggtg
     2581 atgcaccagg ctgtataatt cttttttaaa aaaatagtat ttaacagatt ttttcataaa
     2641 aaactgtttt tctgcattgt atttattaca tttgcaaaag ggctattatt gttttaattt
     2701 aattttaaac aaatgaaatt tatcagagta actttagtgt ttttattcat agttacattg
     2761 tttacggcat gtaaaaaaga agggccacag ggccctgccg ggcccgaagg acccgccggt
     2821 tctaccacag cggttaatcc tgccatttac ggaaaatggc aggttacaag cggaatgccg
     2881 gatacaaagt acattatttt gaaaagtaat aatgatattt accgtctcga ctcagccgat
     2941 tacggattca agtctttgac cagctgcatg gcactgctga cttatacaca gatcaatgca
     3001 ttcttaataa cctataatta caccattacc aacaatacac tgacgctttc aaatctgaat
     3061 ggaaccatta tgctgacaaa aaatgacaat gctcctgatg aaacccagtg ggt
//
LOCUS       DAOSFP010000281         3111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29521_length_3111_cov_3.902814, whole genome shotgun sequence.
ACCESSION   DAOSFP010000281 DAOSFP010000000
VERSION     DAOSFP010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29521_length_3111_cov_3.902814"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="PLI16_08170"
     CDS             complement(<1..1234)
                     /locus_tag="PLI16_08170"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOH84573.1"
                     /translation="MKKTALLLIFAVISGMIAAQTPRKDKAVYKEYQPGYYQNYILKG
                     IEEFNTKEEAPKTERRFKMDFSGLNLPTNIELYKSYWHNEPISQGNTGTCWCFSTTSF
                     FESEIYRLTGQKVKLSEMYTVYWEYVEKASYYIKTRGTSAFAEGSEANAVTRMWKKYG
                     VVPLEAYNGMLQGQTYHNHSKMIDEMESYLLSLKKSNAWNEELAINTIKSILNTYLGV
                     PPVTVNIKGKDMTPKEYLTNVLKLNLDDYCDVTSVLEKPFNEKVEYVVPDNWWHSADY
                     YNLPLDDYMKIIKNAIRKGYTVGIGGDVSEAGFEANAQVAMVPTFDIPSEYIDDFARQ
                     FRFGNKTTTDDHGLHIVGYVEKDGKDWYMVKDSGAGSRNVGKQSKSFGYYFFHEDYVK
                     LKMMDIIIHKDMMKDYLPM"
     gene            complement(1374..2426)
                     /gene="porQ"
                     /locus_tag="PLI16_08175"
     CDS             complement(1374..2426)
                     /gene="porQ"
                     /locus_tag="PLI16_08175"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOH84574.1"
                     /translation="MKRFAAILFSVFFYTSLFGQTGGNNTYEFLNLPVSARVAALGGN
                     MLVARDNDLSLAIINPSLIGDNLHHQFSLNFAEYISDVNYGSVAYSHTFKKVGSFAGA
                     LQFINYGRFLEADEAGMTYGNFSGGEYALTLGWGRKLHKNFLLGANLKMIYSQLEKYN
                     SFGLAVDVAGTYFHEKSGFMASLLFKNIGRQLDAYVPGNIEPLPFEIQLGISQRFNKL
                     PLRYSVVYNHIEKWDLTFKDPKTSNVDPVTGDTISPRKVGVFADKLMRHLVLGVEFYP
                     AKRFYISIGYNYQRRHELRTEVKKGIVGFSFGCGINVYKFRISYAWAKYHLKGSPSTI
                     TISTNLTELFSKKEKK"
     gene            2518..>3111
                     /gene="ribD"
                     /locus_tag="PLI16_08180"
     CDS             2518..>3111
                     /gene="ribD"
                     /locus_tag="PLI16_08180"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOH84575.1"
                     /translation="MQQDELFMRRCIDLAMNGLGNVAPNPMVGCVIVHQGLIVGEGFH
                     AVFGGPHAEVAAINAIKNKEILKESTLYVSLEPCCHFGKTPPCTDKIIQTGIKKVVVG
                     IRDPFAQVQGMGIARLREAGVEVVEGVLEADCAALNKRFFTFHQKKRPYVILKWAQSL
                     DGFIDKTRDTDEPHINWITGEKIRLLVHRWRSEEQAIL"
BASE COUNT          795 a          655 c          731 g          930 t
ORIGIN      
        1 acattggcag gtaatccttc atcatatcct tgtggataat gatgtccatc attttcagct
       61 taacatagtc ttcgtgaaag aaataatacc cgaaactctt gctttgttta cccacattgc
      121 gcgagccggc gcctgaatct ttcaccatat accagtcttt gccatctttt tccacatagc
      181 ccactatatg caagccgtgg tcgtcggtgg tggtcttgtt gccaaaccga aactgcctgg
      241 caaagtcatc aatgtattcg gaggggatgt cgaacgtagg caccatagct acctgggcgt
      301 tggcttcaaa tccggcttct gaaacatcgc cgccgattcc taccgtgtaa cctttgcgga
      361 tggcattttt gatgattttc atgtagtcgt caagcggcag gttgtaataa tctgcgctgt
      421 gccaccagtt gtcggggacc acatattcca ctttttcgtt aaagggtttt tcgagcaccg
      481 aagtaacgtc acaataatca tcaaggttta acttcagtac attggtaagg tattctttcg
      541 gtgtcatgtc tttacccttg atattcacgg tgacaggggg cacgcccagg taggtgttca
      601 ggatgctttt gatggtgttg atagccagtt cttcgttcca ggcgttggat ttttttaggg
      661 atagcaggta actctccatt tcgtcaatca tcttgctgtg attatggtag gtttgtccct
      721 gcagcatgcc gttgtatgct tccaggggaa ctacgccgta ttttttccac atacgggtta
      781 cggcgttggc ttccgaacct tcggcaaatg ctgatgtgcc gcgggtttta atgtaatagg
      841 atgctttttc gacatattcc cagtacacgg tgtacatctc cgaaagtttt actttctggc
      901 ctgtaagacg gtatatttct gattcaaaaa atgaggtggt ggaaaaacac cagcacgttc
      961 ctgtattgcc ttgcgatatg ggctcgttat gccagtagct tttatacaat tcgatgttgg
     1021 tgggcaggtt tagccccgag aaatccattt tgaaacgcct ttccgttttt ggggcttctt
     1081 ctttggtgtt aaactcttca atacctttta aaatgtagtt ttgatagtat cccggctgat
     1141 attctttata tacggccttg tcttttcggg gtgtctgggc cgcaatcatt ccgctgatga
     1201 cagcaaatat cagtaataaa gcagttttct tcatgttgat taaatttatg ctatatgcaa
     1261 aagtacaaaa aaaaggcact tcggtgatgt ttcccgggat taatagcaat atagaataag
     1321 tttgctgtcc tcagttttct gaaggtacta tttcttcaat gaaaatgctg tacttatttt
     1381 ttttcttttt tcgaaaacag ctctgtcagg ttggtggaaa tggttatagt gctgggcgat
     1441 cccttcaggt gatattttgc ccacgcatag ctgatacgga atttatatac gttgatgccg
     1501 caaccgaacg aaaacccgac gattcctttt tttacttcgg tgcgcagttc gtggcggcgc
     1561 tggtagttat atcctatgct gatgtaaaag cgtttagccg gataaaattc cacacccagc
     1621 accaggtgcc tcatcagttt atcagcaaaa acgccgactt ttcttggact aatagtgtct
     1681 cctgtaacgg ggtccacatt tgaagttttt gggtctttaa acgtgaggtc ccatttttcg
     1741 atgtggttgt aaaccactga gtatcgcagg ggtaatttat tgaatctttg cgaaatgcct
     1801 agctgaatct caaaaggcag tggttcaata ttgcccggga cataggcgtc gagttgccgg
     1861 ccaatatttt taaacagcaa acttgccata aaaccgcttt tttcgtgaaa gtaagttcca
     1921 gccacatcta ctgccaggcc aaaagaattg tatttttcca gttgcgaata aatcatcttc
     1981 agatttgctc ccagcagaaa attcttatgt agtttccggc cccagcccaa cgtaagggca
     2041 tattctccac cggaaaagtt gccataggtc atgccggctt cgtcggcttc gagaaaacgt
     2101 ccgtaattga taaactgcaa agctccggca aaactgccca ctttcttgaa agtatgcgaa
     2161 taggcaaccg aaccgtaatt cacgtcgctg atatactcgg caaagttcaa cgaaaactga
     2221 tggtgcaggt tgtcacctat cagcgagggg ttgataatgg cgagagataa gtcgttgtct
     2281 ctggcgacaa gcatgttgcc tccaagtgcg gccacacggg cggaaaccgg aaggttgaga
     2341 aattcgtagg tgttattgcc tccggtttgt ccgaacaggg aagtatagaa aaaaactgaa
     2401 aaaagtatag ccgcaaatct tttcaaaagt cagtgtttta tgaagcaaaa ataatgtttt
     2461 ttgttgctcc tgcctgacat aacggaaatt aattaaaaat attgtacttt tgacaccatg
     2521 cagcaggatg aattgtttat gaggcgttgt attgatctcg ccatgaatgg attgggaaat
     2581 gtggcaccca accctatggt aggctgtgtc attgttcatc aaggccttat tgtcggcgag
     2641 ggctttcatg cggtttttgg cggtcctcat gccgaggttg ctgctatcaa cgctattaaa
     2701 aacaaagaaa ttctgaagga aagtacctta tatgttagtc tggagccctg ctgccatttt
     2761 ggaaaaacac cgccctgcac tgataagatt atccaaacag ggatcaaaaa agttgtggta
     2821 ggcatcaggg atcccttcgc acaggtgcag ggcatgggaa ttgcacgact gcgcgaagca
     2881 ggggtggaag ttgtggaagg cgtgctggaa gcggattgtg cggcgctgaa caaaaggttt
     2941 ttcacttttc atcaaaagaa gcgcccttat gttatactga aatgggcgca aagccttgat
     3001 ggttttattg ataaaacccg tgatactgat gaaccgcata taaactggat aaccggcgag
     3061 aaaatccgtt tgctggtgca tcgttggcgc tcggaagaac aagctatact g
//
LOCUS       DAOSFP010000282         3098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29698_length_3098_cov_4.103516, whole genome shotgun sequence.
ACCESSION   DAOSFP010000282 DAOSFP010000000
VERSION     DAOSFP010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29698_length_3098_cov_4.103516"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="PLI16_08185"
     CDS             <1..120
                     /locus_tag="PLI16_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOH84576.1"
                     /translation="KEYFSEISMGMSSDYMIAVEEGSTMVRVGTAIFGERNYL"
     gene            complement(111..1133)
                     /locus_tag="PLI16_08190"
     CDS             complement(111..1133)
                     /locus_tag="PLI16_08190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH84577.1"
                     /translation="MKYSIIIATYNRLEEVKELLASAENLEFPREKFEFVFSDDGSTD
                     DTENFLRSYQSASHLNIRYLYQQNKGPGEARNHGMRESQGEYFIFVDSDCMFPPEWLR
                     KIDDHLAQDPLDSWGGPDTCHESFSPLLKAINYSMTSFIGTGGTRGSTKSVQKKFYPR
                     SFNMGVSKKVFETIGGMGKLRHGQDMEFSGRIYNHGFKVGLIADAFVYHKRRTSLKRF
                     YKQVFNWGVARVNLGRIDKQMFKPVHFLPALLIAGFVLLVVLTPFPFFPKWLWLIVLA
                     GVLGIAAIAFLQSFAKYKNLKTSLLSIITLYIQIIAYGLGMWKGLFQVWTGKKTASGY
                     SKNYYK"
     assembly_gap    1333..1432
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1473..>3098)
                     /gene="asnB"
                     /locus_tag="PLI16_08195"
     CDS             complement(1473..>3098)
                     /gene="asnB"
                     /locus_tag="PLI16_08195"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOH84578.1"
                     /translation="EEEGNTFFHKLNGIFAFALLDKNKDRLLLVRDHFGVKPLHYYFN
                     NGLLIFGSEQKSILLHPAVSRSINYNALHSHLNLRYTQRNETLFKGIKRLPPAHFLLF
                     ENGQLHIKRYWELTPEPDTEMNEDAAIEKMHFYIKQAIDRQLMSDVPLGVYLSGGMDS
                     STIVQKMHELGVPKINTFTMGFNEPTDEFPDAEHIAKHFGTNHHTLSLTLNPMQQFPE
                     VIWHAEEPKINLLQGFNMSKFVRKEITVVLGGLGGDELFAGYDIHKFIYPFNKYHTHT
                     PEWLQKILRWKSGLIFNMQNSTHTLTIDEYRRGMQMLLAIGDIERFYLILRNVWDYDN
                     RFYKEIYHQNFFKQMQEQCQKVHTEFDGFFARSKNLNALDQVLFTEFQSKMVNDYLLT
                     EDRMSLANSVEERVPFLDLDLVNFGFSIPVHLKIKNNQTKYLFRKAMAQKLPPKIITK
                     KKWGFSVNPYIQFQKDLKTTAEAILTPEFIEKQGIFNYQYIKRIIDHSPHPKMRWHYN
                     YLWIVTGLAIWQKMYIDSDIFKTKEIDIKNYFS"
BASE COUNT          822 a          607 c          644 g          925 t
ORIGIN      
        1 aaggagtact tttcggagat ttctatgggt atgagcagcg attacatgat tgccgttgaa
       61 gaaggctcaa ccatggtaag ggtgggaacg gcaatttttg gcgaacggaa ttatttgtaa
      121 taattctttg aatatcccga ggccgttttt ttgcctgtcc atacctggaa cagtcctttc
      181 cacatgccca gaccataagc aataatctgt atgtacaaag tgattattga aagcaatgat
      241 gttttcagat ttttatactt ggcaaatgac tgcaaaaaag caatagccgc aatgcccagc
      301 acaccggcaa gaacaatcag ccaaagccat tttggaaaga aaggaaatgg cgtaagcaca
      361 acaagaagaa caaatcctgc aatgagcagg gccggcagaa aatgcaccgg tttgaacatc
      421 tgtttatcta tcctgcccag gttcacccgg gccacgcccc agttgaacac ctgtttgtaa
      481 aaacgtttta gcgaggtacg gcgtttatga tatacaaagg catcggcgat aaggcctact
      541 ttaaaaccat gattgtaaat tcttcccgag aactccatat cctgaccgtg tctgagtttt
      601 cccataccac ctatggtctc aaatactttt tttgaaacac ccatattgaa acttcgggga
      661 tagaattttt tctgaacaga tttggtgctt ccacgggtgc caccggtgcc tataaaagag
      721 gtcattgaat agttgatggc tttcagcaat ggcgaaaagc tttcatggca ggtatcaggg
      781 ccgccccagg aatccaaagg gtcttgtgcc agatgatcgt caatcttgcg gagccattca
      841 ggcggaaaca tacaatcgga atctacaaaa atgaagtatt cgccttgtga ttcgcgcatg
      901 ccatggttac gcgcttcgcc gggacctttg ttttgctgat acaaatagcg gatattcaga
      961 tgactggccg attgatagct cctgagaaaa ttctctgtgt cgtctgtgga gccatcgtca
     1021 gaaaagacaa attcgaattt ttctcttgga aactctagat tctcggcaga agccagcaat
     1081 tctttcacct cttccaaccg gttgtatgta gcgattatta ttgagtattt catattattt
     1141 gtaattgagc ctctgcgtct ttttgagaat attaaattaa aaatataact tagatttcag
     1201 tctccctttc ttagcgcctt agcgtctttg cgagaaaatt aagtaataaa aataacttcg
     1261 gtttttgttt tcctttttta gcgccttagc gtctttgcga gaaaattaag taataaaaat
     1321 aacttcggtt ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgttttcc
     1441 ttttttagcg cctttcgtct ttgcgggatt aattatgaaa aataattttt gatgtcaatt
     1501 tctttggttt taaatatgtc tgaatcgata tacatttttt gccatattgc caaccccgtc
     1561 acaatccaca gataattgta gtgccagcgc atcttggggt gaggggagtg gtcaatgatg
     1621 cgtttgatat actgataatt aaaaattccc tgtttttcga taaattcagg tgtgagaata
     1681 gcttcggcag tggtttttaa gtctttttga aactgtatgt aaggatttac cgaaaagccc
     1741 cattttttct tggtaatgat ttttggcggg agtttttgtg ccatggcttt cctgaaaagg
     1801 tatttcgtct gattgttttt tattttgaga tgaacaggga ttgaaaaacc aaaattcacg
     1861 aggtcaaggt ccaggaatgg cacacgttct tcaaccgagt tggccagcga catgcggtct
     1921 tctgtaagca ggtagtcatt caccatttta ctctgaaact ctgtgaataa cacctgatcc
     1981 agtgcattta agttttttga ccgtgcgaaa aatccatcaa attcagtgtg cactttctga
     2041 cattgttcct gcatttgttt gaaaaaattc tgatgataaa tttccttgta aaatctatta
     2101 tcgtaatccc aaacattccg caaaataaga taaaatcttt cgatatcgcc tatggcaagc
     2161 aacatctgca tccctcgccg gtattcgtca atggtcaggg tgtgcgtgga attttgcatg
     2221 ttgaaaatca aacctgattt ccatctgagg attttttgca accattcagg cgtatgtgtg
     2281 tggtatttgt tgaaaggata aataaattta tgaatatcgt aaccggcaaa aagctcgtca
     2341 ccacccaaac cgccaagaac aaccgtgatc tctttcctga cgaatttcga catgttgaaa
     2401 ccctgaagta ggtttatctt aggctcttcg gcatgccaga taacctctgg aaactgctgc
     2461 atcggattta gtgtcagtga aagggtgtga tggttggtgc caaaatgttt cgcaatatgt
     2521 tctgcatcgg gaaattcatc cgttggctcg ttgaaaccca tggtaaaggt gttgatcttc
     2581 ggcacaccca gctcatgcat tttctgtaca atggtggacg aatccatccc gcccgagagg
     2641 tatacgccca gagggacatc gctcatcaac tgcctgtcga tggcttgttt gatataaaag
     2701 tgcatctttt cgatggcagc atcctcgttc atttcagtgt cgggttcagg cgtgagttcc
     2761 caataacgct ttatatgtaa ttgcccgttt tcaaacagca ggaaatgtgc cggcggcagt
     2821 cgttttattc ctttaaaaag cgtttcattt cgttgggtgt acctgagatt caggtgcgag
     2881 tgaagcgcat tgtaattgat gctgcggctg acagccggat gaagcagtat gcttttttgt
     2941 tccgagccga agatcagcaa accattgtta aagtagtaat gcaaaggttt tacaccaaaa
     3001 tggtcgcgca ccagcaacaa tcggtctttg ttcttatcta acagtgcaaa agcaaaaata
     3061 ccattcagtt tatggaaaaa tgtgttgcct tcttcctc
//
LOCUS       DAOSFP010000283         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29787_length_3092_cov_3.219295, whole genome shotgun sequence.
ACCESSION   DAOSFP010000283 DAOSFP010000000
VERSION     DAOSFP010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29787_length_3092_cov_3.219295"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PLI16_08200"
     CDS             <1..362
                     /locus_tag="PLI16_08200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOH84579.1"
                     /translation="NIYMNADNNEMEIIFNNLISNAVKYNKTNGTVEIKIATDDNKIS
                     IAVSDTGIGMSEDDIAKLFQEFSRIKNEKTKGISGSGLGLSILKKTVEDFYHGTVTVK
                     STPDVGSTFTVEIPKNY"
     gene            391..1818
                     /gene="hydG"
                     /locus_tag="PLI16_08205"
     CDS             391..1818
                     /gene="hydG"
                     /locus_tag="PLI16_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOH84580.1"
                     /translation="MKFTPEKYKIPDVPMQPFIDAEEIEEYLNNAVSTKESVRAIIAK
                     SLSKKRLSLAETAILINTTDPELVEEIKDGARQLKKLVYGNRIVLFAPLYIGNKCANN
                     CTYCGFRASNTAAKRVTLTDEEIVNEVKSLEDNGQKRLILVYGEHAQYNPEFIAHTVR
                     LAYSVKKNNGEIRRVNINAAPLDIEGFKVVKEAGIGTYQVFQETYHPEAYKTYHLRGK
                     KADYGYRLTSLDRAQEAGLDDVGIGALFGLYDWRFEVMGLVRHTNHLEACYNVGPHTI
                     SFPRIKDASMLSMGDKYFVNDGDFAKLVAILRLAVPYTGMILTAREPVALRNEVIQFG
                     VSQIDGGTKLELGSYSESEHVEQDLNRGQFRINDSRTLSEIISELLDQEMLPSFCTAC
                     YRLGRTGEHFMEFSVPGFIKRFCTPNAILTLAEYLVDYGNELNSEKGWKLIEKELQAM
                     GDEKNVAEIRNRIELIKNGKRDLYF"
     gene            1928..>3092
                     /gene="hydF"
                     /locus_tag="PLI16_08210"
     CDS             1928..>3092
                     /gene="hydF"
                     /locus_tag="PLI16_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOH84581.1"
                     /translation="MSKGRDLKPQIGIFGRRNNGKSSLINLLVGQEVAIVSEQPGTTT
                     DPVRKSIEIFGIGPVIMVDTAGIDDAGSLGSKRIEKSLQVIKTIDCAVLVITENFFGQ
                     PEVKLIEDFNRYNIPFIVVHNKSDLQALSAATRELVQKYTKQDIIEFSTLKPDNLEVL
                     VSALKNAIPESAYLSRSLVGDIINEGDYVLLVTPIDSEAPEGRMILPQQMAIRDVLDN
                     NCVCIVLKETELQHFFKTSCIKPVLVITDSQVFGTVKELVPEDIPLTSFSIIFSRLKG
                     DFENYVNGTQTISSLNDGDRILILESCTHHVSCDDIGRVKIPMWLSKFTGKKLYFSNV
                     SGLDALPENFKDYKLVIQCGGCMVTRKQILNRLKPFTENSVSITNYGMAIAFMN"
BASE COUNT         1010 a          643 c          673 g          766 t
ORIGIN      
        1 ctaacatata catgaatgcc gacaacaacg aaatggaaat aatctttaac aaccttattt
       61 ctaacgctgt caaatacaac aaaaccaacg gtacggtgga aattaaaata gctaccgatg
      121 acaacaaaat ttctattgcg gtttccgata ccggtattgg aatgtcggaa gatgatatcg
      181 caaaattgtt ccaggagttt tcacgtatca aaaacgagaa aaccaaaggc atcagcggca
      241 gcggactcgg actttcgatt ttgaaaaaaa ccgtggaaga tttttatcat ggaactgtaa
      301 cagtgaaaag cacgcccgat gtgggaagta cttttactgt tgaaattcct aagaattatt
      361 aataaacttt tgcagaaaac gaggtgccaa atgaaattta ctcctgagaa atacaaaatt
      421 cctgatgtcc ccatgcagcc ttttatcgac gctgaagaaa ttgaggagta tttaaataat
      481 gccgtatcca caaaagaatc tgtcagggct atcattgcaa aatccttatc gaaaaaacgc
      541 ctgtcgctgg cagaaactgc cattctgatt aatactaccg accctgaact tgtcgaagag
      601 ataaaagatg gagcacggca actgaaaaaa ttggtatatg gcaacaggat agtgctgttt
      661 gcccccctct acatcggtaa taaatgtgca aacaactgca cctattgtgg cttcagagct
      721 tccaataccg ctgcaaaaag ggtaaccctc accgacgaag agattgttaa tgaggttaaa
      781 tcgctggaag ataatggaca aaaaagatta attctggttt atggcgaaca tgcacaatac
      841 aacccggaat tcattgccca taccgtccgt cttgcctatt ctgtcaagaa aaataatggc
      901 gaaataaggc gcgtgaacat taatgctgcg ccactggata tagaaggttt taaggttgtt
      961 aaagaagcag gcataggcac atatcaggtt tttcaggaaa cataccatcc agaggcttat
     1021 aaaacctacc acctgagggg taaaaaagct gattacggat accggcttac ctcgctggac
     1081 cgtgctcagg aagccggcct tgatgatgta ggcatcggcg cactttttgg attatacgac
     1141 tggcgttttg aggttatggg actggtgcgt cataccaacc atctcgaagc ctgctataac
     1201 gttgggccac ataccatttc atttccccgg attaaagatg cttccatgct cagcatgggt
     1261 gacaaatatt ttgtgaacga cggcgatttt gcaaaactgg tggccatact gcgactggca
     1321 gtgccttata ccggcatgat attaaccgcc cgcgagcctg tggcactgcg caacgaagtc
     1381 attcagtttg gtgtgtcgca aatcgacgga ggcaccaaac tggaacttgg cagctattcc
     1441 gaatcagaac atgtggaaca agacctgaac agaggccagt tccggatcaa cgacagccgg
     1501 acactgagtg agattatttc ggaactcctc gaccaggaaa tgctgccttc attttgcacc
     1561 gcatgctacc ggctgggaag aacaggcgaa cattttatgg aattttcggt tcccggcttt
     1621 ataaaacgct tttgcacccc caatgccata cttaccctgg ccgagtacct ggtggactac
     1681 ggcaacgaat taaacagcga aaagggctgg aaacttattg aaaaagaact gcaggcaatg
     1741 ggcgacgaaa aaaatgttgc tgaaatcaga aataggatag agcttattaa aaacggaaaa
     1801 agagatttgt atttctaaat taccagaaaa acaacaataa ccggtttaac cgacaaatgc
     1861 cggcaaatgt tgatacaact tacacagaaa aatgtgtaaa taaaataaga ggcaaacaac
     1921 cagtattatg agcaagggaa gggatttaaa accacaaata ggtatctttg ggagacgcaa
     1981 caacggcaaa agctcattga taaatttgct cgtagggcag gaggtggcta ttgtgtcgga
     2041 acagccaggc actaccaccg acccggtgcg aaaatccata gagatatttg gcattggccc
     2101 tgtgataatg gttgataccg ctggtattga tgatgcaggc agcttgggat ccaaaagaat
     2161 tgaaaaatca cttcaagtta taaaaaccat tgattgtgcc gtactggtca taacggaaaa
     2221 tttctttgga caaccggaag taaaactgat agaagatttt aacagataca acattccttt
     2281 tatcgttgtc cacaataaat ctgacctgca agccttatcg gcagccacac gcgaacttgt
     2341 acaaaaatat accaaacaag acataatcga gtttagcacc ctcaaacccg ataacctgga
     2401 agtactggtg tctgccctga aaaatgctat acccgaatcg gcatacctta gccgttctct
     2461 ggtgggcgat atcatcaacg aaggagacta tgtactgtta gtaaccccta ttgactcgga
     2521 agctcccgaa ggccgcatga ttttgcctca gcaaatggca atacgggatg tgctcgacaa
     2581 taactgtgta tgcatagttc ttaaggaaac agaacttcag cattttttta aaacctcttg
     2641 cataaaacct gtgctggtta taactgacag ccaggttttc gggacagtca aggaactggt
     2701 gcctgaagac atacccttga caagtttcag cataattttt tcgcgtctga aaggcgactt
     2761 tgagaattat gtgaatggaa cacaaaccat aagctcactc aacgacggtg acaggatact
     2821 tatactcgaa tcatgtacgc atcatgtcag ctgtgatgat atcggaagag tgaaaattcc
     2881 tatgtggtta tcaaagttta caggaaaaaa attatatttt tcaaacgtat ccggtttgga
     2941 tgcgttgccg gaaaatttca aagactataa attggttata caatgtggag gctgcatggt
     3001 gacccgcaaa caaatactaa acagactaaa gccatttaca gaaaattcgg tttccataac
     3061 taattacgga atggcaattg cttttatgaa cg
//
LOCUS       DAOSFP010000284         3089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29819_length_3089_cov_4.095913, whole genome shotgun sequence.
ACCESSION   DAOSFP010000284 DAOSFP010000000
VERSION     DAOSFP010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29819_length_3089_cov_4.095913"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /gene="rho"
                     /locus_tag="PLI16_08215"
     CDS             complement(<1..1301)
                     /gene="rho"
                     /locus_tag="PLI16_08215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOH84582.1"
                     /translation="MLPSLDEDETTVRPVQPADEESVKPGTQETPVAETAHAASAGEN
                     GTSNVEEIKEPAAEENKTNEQERVQRDHMNRNRPQREKQPEYSFDFEGIVSSEGVLEI
                     MPDGYGFLRSSDYNYLNSPDDIYVSQSQIKLFGLKTGDTVKGRIRPPKEGEKYFPLVK
                     VEHINGRSPEIVRDRVNFDHLIPLFPEKKLKLTGHPQMTVTSRIIDLFTPIGKGQRGL
                     IVAQPKTGKTMILKEIANAVAHNHPEVYLIILLVDERPEEVTDMQRSVNAEVIASTFD
                     EPADRHVKIANIVLEKAKRLVECGHDVVILLDSITRLARAYNTVAPASGKVLSGGVEA
                     SALQKPKKFFGAARQIENGGSLTIIATALTETGSKMDEVIFEEFKGTGNMELQLDRKL
                     SNKRMFPSIDLVASSTRRDDLLHEKDVLQRIWILRNHLADM"
     gene            complement(1515..1841)
                     /locus_tag="PLI16_08220"
     CDS             complement(1515..1841)
                     /locus_tag="PLI16_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOH84583.1"
                     /translation="MYNIIDLNGMSIQELQEIAEKLNIPDYEDLRKKELIHKILDTQA
                     LKPEEPKPREKSPEENIKRRRVTQRRARIENPELKQQAGNGQLKPEGFAQPQDNPSNN
                     SEKRQR"
     gene            complement(2004..>3089)
                     /locus_tag="PLI16_08225"
     CDS             complement(2004..>3089)
                     /locus_tag="PLI16_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH84584.1"
                     /translation="PDSTPFANQQYLELTTKKPGFTVTMLRVCKNEPQEKTGFFKKML
                     YGEKTRYQDIPIERYYFEGDKIFVEEYTGLTPNNGEIKSFSLADVLYALPPDSQKIFT
                     AKTTVTFTDIEGKTITEDISSLQQKTETEHIFTKKFLLGSDRFGRDVLSQIMIGTRVS
                     LSVGFISVFISLLVGILLGALAGYFRGWVDNLIMWFINVVWSIPTLLLVIAITFVLGK
                     GFWQIFIAVGLTMWVEVARVVRGQILSIREKEFVEAGVALGFTNTRIIFRHILPNVLA
                     PVIVISAANFASAILIESGLSFLGIGVQPPMPSWGTMIKEHYGYIILDSAYLAIAPGI
                     AIMLTVLAFMLTGNGLRDAMDVKMVNK"
BASE COUNT          692 a          646 c          777 g          974 t
ORIGIN      
        1 ttcatgtcgg ccaggtggtt gcgcaaaatc catatacgct gtagaacatc tttttcgtgg
       61 aggaggtcgt cgcggcgtgt gcttgacgct accagatcga tagacgggaa catgcgtttg
      121 tttgagagtt tgcggtcgag ctgaagttcc atattgccgg ttcctttgaa ttcttcaaag
      181 atcacctcgt ccatttttga tccggtttcg gtgagggcgg tggcgataat ggtgagcgaa
      241 cctccgtttt ctatttggcg tgcagcgccg aagaattttt tgggtttttg cagggccgag
      301 gcctccacgc cgcccgagag tacttttccg gaggctggcg ccacggtgtt ataagccctt
      361 gccagacggg tgatcgagtc gagcaggatc accacatcgt gtccgcattc aaccaggcgt
      421 ttggcttttt caaggacaat gttggcaatt tttacatgac ggtcggccgg ctcatcaaaa
      481 gtagaagcga ttacttccgc atttacgctg cgttgcatat ctgtaacttc ttcggggcgt
      541 tcatcaacca gcagtatgat gaggtacact tcgggatggt tgtgggcaac ggcattggca
      601 atttctttaa gaatcatggt tttacctgtt ttgggttgcg ccacgatgag gcctcgctgt
      661 cctttgccga taggggtgaa caggtcgatg atgcgcgaag taaccgtcat ctgaggatgt
      721 ccggtaagtt tgagtttctt ttcaggaaac agggggatga ggtggtcgaa attaacacgg
      781 tcgcggacta tttcggggct tcggccgttg atgtgttcca cttttaccag gggaaaatat
      841 ttctctcctt ctttgggcgg acggattctg cctttaacgg tgtctccggt ttttagtccg
      901 aagagtttga tctgcgattg cgaaacataa atgtcgtcgg gggagttgag ataattatag
      961 tcagaagaac gtaaaaaacc atagccatca ggcataattt cgagcacgcc ttcgctggaa
     1021 acgatacctt cgaagtcgaa cgaatattcg ggttgttttt cgcgttgggg ccggttgcgg
     1081 ttcatgtggt cgcgctgtac gcgttcctgt tcattggttt tgttttcttc ggctgcgggt
     1141 tctttgattt cttcaacgtt acttgtgccg ttttctccgg cgcttgcagc atgggcggtt
     1201 tcggctaccg gggtttcctg agtgccgggt tttacagatt cttcatcagc aggctggaca
     1261 ggtcgtacag tcgtttcgtc ttcatccagc gacggcagca taaaatcaaa ctttttggct
     1321 tcttcttcaa aaagttcttt tttataatcc ttatcgattt ttggctcctg ggttttgttt
     1381 tcttcagtac tgaaaactat ttcgggttgt ttctcctgag ttatttgctt ttttggtgtg
     1441 ttttcaggtt tttcttcaac tttttctata ggggtatttt tttcaaaaac cttatgttta
     1501 ttgaagtttt tcggttatct ttgccttttt tcagaattgt ttgacggatt atcctgaggt
     1561 tgtgcaaaac cttccggttt gagttggccg tttccggctt gttgtttcag ttcagggttt
     1621 tctatgcgcg ctctgcgttg ggttacacgg cggcgtttta tattttcttc cgggcttttt
     1681 tcccgtggtt ttggttcttc tggttttaat gcctgcgtat cgagaatttt gtggataagc
     1741 tctttttttc taaggtcttc gtaatcggga atgtttagtt tttctgctat ttcctgtagc
     1801 tcttgaatgc tcattccatt caggtcaatg atgttataca taaaatgtag attggttaag
     1861 aattagtcaa tttggtgaaa atagtttgta aaatgctggt taaatgaaat gtttcaggta
     1921 acgaatgtta gtgaaacaga attgcaaacg gggaaattcg gtgcaaatat accaactttg
     1981 attaaaaaat gaaaaagaaa attttatttg tttaccattt tcacatccat ggcatcacgc
     2041 aggccattgc ctgtgagcat aaaggccagc acggtgagca ttatggcaat gcccggagca
     2101 atggcaagat aagccgaatc aaggatgatg tatccgtaat gttctttgat catggtaccc
     2161 cacgagggca tgggtggctg aacgccgatg cccagaaaac tcaggccgga ttctatgagt
     2221 atggccgaag caaagttggc tgccgagatt actatcactg gtgccagtac gttcggtaaa
     2281 atgtgccgga agatgatgcg tgtgttggta aacccaagcg ccacgccggc ttccacaaat
     2341 tctttttcgc gtatgctgag aatctgtccg cgcaccaccc gggccacctc tacccacatg
     2401 gtgaggccca ccgcgataaa tatctgccaa aaacccttgc ccaatacaaa cgtgatggca
     2461 ataaccagca gtaaggtagg aatggaccac accacattga taaaccacat gatgaggttg
     2521 tcgacccaac ctctgaaata tcctgccagg gctcccagca aaatgccgac cagcagcgaa
     2581 ataaacaccg aaataaaacc taccgacagg ctcacgcggg tgcctatcat aatctggctg
     2641 agcacatcgc ggccaaaacg atcactgcct aataaaaact tttttgtgaa gatgtgttcg
     2701 gtttctgttt tctgttgcag gctgcttatg tcttcagtga tggtcttgcc ttcaatatcg
     2761 gtaaaagtaa ccgttgtttt ggccgtaaag attttttgtg agtcgggagg cagggcgtac
     2821 agtacatcgg caaggctaaa agattttatt tcaccattgt tgggtgtcag gcccgtatat
     2881 tcttctacaa atattttatc accttcaaaa taataccttt cgatggggat gtcctggtag
     2941 cgggtttttt cgccgtaaag cattttttta aagaaacccg ttttttcctg tggttcgttt
     3001 ttgcataccc tgagcatggt tacggtaaat cccggttttt tggtggtcag ttccaggtac
     3061 tgttggttgg caaaaggtgt ggaatcagg
//
LOCUS       DAOSFP010000285         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_29908_length_3083_cov_3.475561, whole genome shotgun sequence.
ACCESSION   DAOSFP010000285 DAOSFP010000000
VERSION     DAOSFP010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29908_length_3083_cov_3.475561"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLI16_08230"
     CDS             <1..429
                     /locus_tag="PLI16_08230"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84585.1"
                     /translation="TIRAEGRVVNGKIDGLWRTYFEDGQIQSAITYKDGMAEGLAFFY
                     YDNEKQTTRVELTFGEDKVVGVYREFYENGNRKAMLNFNEGQPDGVAEFYYDSGVIKI
                     AGRYKSGAKEGKWKHYNETGELISKDKWKKGEQKIKNEED"
     gene            517..1104
                     /gene="nadD"
                     /locus_tag="PLI16_08235"
     CDS             517..1104
                     /gene="nadD"
                     /locus_tag="PLI16_08235"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOH84586.1"
                     /translation="MNTTAKVVGLYLGSFNPIHKGHLSIARQVLKTAGLHEVWFVISP
                     QSPFKSVAALLDDDYRYEMVRLAIKNNPRLRACNIEFGLPQPSYTINTLKALKEKYPE
                     TEFCIIIGSDNLDAFDKWKNYEDIIGQYRLLVYPRAGSDGGRFRHHPSVTWIEAPLLD
                     ISSTAVRAAVNQGQDVSKLLPAMVLRYINKMGFYK"
     gene            1219..2433
                     /locus_tag="PLI16_08240"
     CDS             1219..2433
                     /locus_tag="PLI16_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOH84587.1"
                     /translation="MQHLPSINISEYDYPLPDERIARYPIPLRDASKLLYYTNGVITD
                     KHFFDLPDLLAQDTLLVMNNTRVIHARMNFRKETGAKIEIFCLEPVSPVRDVQLAMQQ
                     TGACTWKCLVGNAKKWKGEKLFMQVGEGNSAYRLCAELCGRENESFLVHFSWFPEQIL
                     FSDVIETAGSVPLPPYIHRPAEQNDNERYQTVYARRDGSVAAPTAGLHFTPAVLEKLA
                     EKKIATTYTTLHVGAGTFRPVQSDTIGQHVMHAEQVVVHKKLLTDLLSAKGPLIAVGT
                     TTIRSLESLFLIAAKRYLSGNLTHTLTQWEPYDKELDSLSRDLVLNDLLSFMEKSGLE
                     QFSFSTSIIILPGYRFKMADGMVTNFHQPRSTLLLLVSAMVGDDWKKVYEHALKSDYR
                     FLSYGDACLFLK"
     gene            complement(2489..2950)
                     /locus_tag="PLI16_08245"
     CDS             complement(2489..2950)
                     /locus_tag="PLI16_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84588.1"
                     /translation="METSIPVSTGKHSFFLILLCVFSGFSNLLSLFVTLMVLIYGKSV
                     FFVDSVPVIDIMAEELRHGSIVFYFIKAGVHILCLFALVKLTRGLRKGLILYALCQLL
                     LLALPWLFLQSLGVSYLIMITAISLIFALFFIMLFSLALPKKTEKAPEPLP"
BASE COUNT          929 a          670 c          739 g          745 t
ORIGIN      
        1 acaattaggg ccgaaggccg tgttgtaaac ggaaaaattg acggcctctg gcgtacatat
       61 tttgaagatg gccaaataca gagcgctata acctataaag acggtatggc cgaaggctta
      121 gcattttttt attacgacaa cgaaaaacaa actacccgtg tagagctaac ctttggcgaa
      181 gacaaggtgg tgggtgttta ccgcgagttt tatgaaaacg gcaaccgcaa ggcgatgctc
      241 aacttcaacg aaggacaacc cgacggtgtt gctgaatttt attacgacag cggcgtgata
      301 aaaattgccg gacgttataa aagcggagcc aaagaaggca aatggaaaca ctataatgaa
      361 acaggtgagc ttatcagcaa agacaaatgg aagaaaggtg aacaaaaaat aaaaaatgaa
      421 gaagattaat ctgcagggta tatttttatt gttatcaggt aaatatctta gcagacaagg
      481 attcagggcc gaatctcaac cttatatttg acagccatga acaccaccgc aaaagtcgtt
      541 ggcctgtacc tcggatcttt taatcccata cacaaggggc atctgtctat cgcccgccag
      601 gtgctgaaaa cggcagggct gcacgaagtc tggtttgtta tttcacctca aagtcctttt
      661 aaaagtgtgg ctgccctgct cgacgatgat taccgttatg agatggtgcg gctggccata
      721 aaaaacaatc ccaggctgcg tgcctgtaac attgagttcg ggcttccgca accttcttac
      781 actatcaata ccctgaaagc gctaaaagag aaatatcccg aaacagaatt ctgcatcatt
      841 ataggatctg ataatctgga tgcctttgac aaatggaaaa actacgaaga tatcatcggt
      901 cagtaccgcc tgttggtcta tccccgtgcc gggtcggatg gaggccggtt ccggcatcat
      961 ccctcggtaa catggattga agcgccgctg ctggatatct catcaacggc tgtcagggcg
     1021 gctgtcaacc agggacagga tgtcagcaaa ctcttacctg caatggtttt gagatatatc
     1081 aataaaatgg gtttttacaa ataacatccg atgttttaaa aggactggcg ggagcagcgg
     1141 ttgaggcttt aattcacgag taaaagcttt gcaacaggta ccgcagaaaa accttatttt
     1201 tgcaccatag aaaaaagtat gcagcacctt ccatcaataa atatttcgga gtacgactat
     1261 ccgctgcctg acgaacggat agcccgctat cccataccgc tgcgcgacgc ctcgaaactg
     1321 ctgtattaca caaacggagt gattaccgac aagcattttt ttgacctgcc cgatctgctg
     1381 gcgcaggata cactcctggt gatgaacaat acccgcgtga tacatgcccg tatgaatttc
     1441 agaaaagaga ccggggcgaa gatcgagata ttttgtctgg aaccggtatc gccggttcgt
     1501 gatgtgcaac tggccatgca gcaaaccggc gcctgtacat ggaaatgcct ggtgggaaat
     1561 gccaaaaaat ggaaaggtga aaaacttttt atgcaggtag gtgagggcaa tagtgcttat
     1621 cggttgtgtg cagaattgtg tggccgcgaa aacgagagtt tcctggtgca tttcagttgg
     1681 tttcctgaac aaatcctgtt ttctgacgtg atagaaaccg caggtagtgt gccgcttcct
     1741 ccatatatac acaggcctgc cgaacagaac gataacgaac gctaccagac ggtgtatgcc
     1801 cgcagggacg gctctgttgc ggcccccacg gcagggttgc attttacgcc tgccgtgttg
     1861 gaaaaactgg ccgaaaaaaa aattgccact acctatacca ccctgcatgt tggcgcagga
     1921 actttcaggc cggtgcaatc cgacactatc ggccagcatg tgatgcatgc cgaacaggta
     1981 gttgtacata agaaactgct taccgacctg ctgtcggcca aaggcccgct gatagctgtt
     2041 ggcactacca ccatacggtc gctggaaagt ttgtttttaa tagcggcaaa aaggtatctc
     2101 agcggtaacc taacccacac gctgacacaa tgggaaccct acgataaaga gctggattca
     2161 ctctccaggg atttggtgct taacgacctg ctcagtttca tggaaaaatc aggcctggag
     2221 cagttttctt tttcaacaag catcatcatt ttaccggggt accggtttaa aatggccgat
     2281 ggcatggtta ccaactttca tcagccccgc agtaccttgt tactattggt ttctgccatg
     2341 gtgggcgacg actggaaaaa ggtatatgag cacgccctca aaagcgatta ccgttttttg
     2401 agctatggcg atgcttgtct ttttctgaaa taatatcagt tttaattgtt aagagtagct
     2461 gctattgtgg gttgccaggg ataatttttc agggcaaagg ttccggtgct ttttctgttt
     2521 tttttggcag cgccagcgaa aatagcatga taaaaaacag ggcaaaaatc aatgaaatgg
     2581 cggtaatcat tatcaggtag gagacaccca gcgattgaag aaacagccag gggagggcta
     2641 gcaacagcaa ctggcacagc gcatacagaa tcaggccttt tcggaggcct ctggtcagct
     2701 ttaccagagc aaaaagacag agaatatgca caccggcttt gatgaaataa aaaactatgg
     2761 agccgtggcg taattcttcg gccataatat caataaccgg caccgaatct acaaaaaaga
     2821 cagatttacc atatatcaat accatcaggg taacaaacaa cgagaggaga ttagagaatc
     2881 ctgaaaagac acacaaaaga attagaaaaa agctatgttt accggttgat accggaatgg
     2941 atgtttccat ggattatttt ttcaggtatt tatattgtaa ggagtataaa acaatgaata
     3001 ccacgctgcc taatatgaag atcgcattaa aaaaagtcat cactacaaaa aaagagagaa
     3061 agagcatgat tacattggcg gcg
//
LOCUS       DAOSFP010000286         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30009_length_3075_cov_3.538079, whole genome shotgun sequence.
ACCESSION   DAOSFP010000286 DAOSFP010000000
VERSION     DAOSFP010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30009_length_3075_cov_3.538079"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLI16_08250"
     CDS             complement(<1..473)
                     /locus_tag="PLI16_08250"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOH84589.1"
                     /translation="MSEWKEYKLGDVITFQRGHDLPKTEMKDGLYPVAGSNGIIGYHN
                     NFTTKGPGITIGRSGSIGTPFFYEHDYWAHNTVLYVKDFKGNFQKFIFYFLKTLDFTQ
                     FNAGSAVPTLNRNHIHDLLVKIPPYDEQTAIASILSSLDDKIDLLHCQNKTLEQMA"
     gene            complement(470..1003)
                     /locus_tag="PLI16_08255"
     CDS             complement(470..1003)
                     /locus_tag="PLI16_08255"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOH84590.1"
                     /translation="MYILQCADGSYYTGSTNNLELRLAQHQTGEGANHTKKRLPVKLV
                     YYEEYQRIDEAFYREKQVQGWRREKKEALIRGDYNLLPELAIAYRDLDTVASSTSATE
                     EITLPEVLEGSTDTVASGASATESASATEEITLPEALEGSVKKTLEGSEKAVSSRASE
                     TAKTRASETAKTKTRKK"
     gene            complement(1070..2233)
                     /locus_tag="PLI16_08260"
     CDS             complement(1070..2233)
                     /locus_tag="PLI16_08260"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOH84591.1"
                     /translation="MNKLPEKNYFEVLDLLKEKIRHTRYRAVITVNYELLKIYWEIGK
                     AILEQQKKAGWGAKIIDRLAVDLKIEFPDFKGLSVRNLKYMRAFAEAYPEFSSFALPP
                     DAPILNTAKTTIVQQAAAQSGNGNTDSFVQQLAAQIPWGHHQVLLDKVKTREQQLFYM
                     QKCVENGWSRSILVEQIESGLYERQGKAISNFRQTLPQLQSDLVQQSLKNPYIFDFIG
                     FGEEIKERELEKGLIQHLKKFMLELGKGFAYVGNQFDLTVEDDEYFLDLLFYNYRLHR
                     FVIFELKIGDFKPEYAGKLNFYINTVDAQIKSNKDDATIGILLCKTPNELVIRYSLQG
                     IKTPIGVSEYLLKKGIPKVLKTDLPSIEELEANIDKEYKGLKRKDNQKNTSKK"
     gene            complement(2230..>3075)
                     /locus_tag="PLI16_08265"
     CDS             complement(2230..>3075)
                     /locus_tag="PLI16_08265"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOH84592.1"
                     /translation="FGQESNQTTYRLCRMNLAIRGIDGSNIKWNNEGSFLRDVHPDLK
                     ADFILANPPFNDSDWSGELLQKDGRWKYGLPPAGNANYAWIQHFIYHLAPSGIAGFVL
                     SKGSLTTNTSGEGEIRKNLIEERLIDCIVNLPDKLFFNTGIPACLWFVTRNKANGKFR
                     NRTNEVLFIDARNLGFMVNRRSREFTDEDISKVASTYHNWRTSTSIGHQDIPGYCKSV
                     TIDEIREKNYVLTPGRYIGLPDEEDDFNFPERFNSLKAEFEKQIAEESVLNKTIMENL
                     KKIQI"
BASE COUNT          758 a          679 c          616 g         1022 t
ORIGIN      
        1 gccatttgct ccagggtttt gttctggcag tggagtaaat caattttatc atcaagcgag
       61 gacaggatgg aggcgatggc ggtttgttca tcataaggcg gaattttaac cagcaagtcg
      121 tgaatatgat ttctatttaa agtgggcaca gcactaccag cattaaattg ggtaaagtca
      181 agtgttttca aaaaatagaa aataaacttt tgaaaattac ctttgaaatc tttcacataa
      241 aggactgtat tatgcgccca ataatcatgt tcataaaaaa atggagtgcc aatgctacca
      301 cttctaccaa tagttatacc tggtccctta gttgtaaaat tgttgtgata tccaataatt
      361 ccattcgaac ctgcaacagg atataaacca tctttcattt ctgtttttgg caagtcatgt
      421 ccacgctgaa atgtaataac atccccaagt ttatattctt tccactcgct cattttttcc
      481 ttgttttggt ttttgcggtt tctgaggctc tcgtttttgc ggtttctgag gctctcgaag
      541 aaaccgcttt ttcgctgcct tcgagagttt ttttcacgct gccttcgagt gcctcaggca
      601 gcgttatttc ctcggtggct gaggcactct cggtggctga ggctcccgaa gccaccgtat
      661 ctgtgctgcc ttcgagaacc tcaggcagcg ttatttcctc ggtggctgag gtactcgaag
      721 ccaccgtatc caaatcccta taagcaatag ccaactccgg caacaaatta taatcccctc
      781 tgatcaatgc ttctttcttt tctctgcgcc acccctgcac ttgtttttcc ctatagaaag
      841 cttcatctat acgttgatat tcttcgtaat acaccagttt taccggcaat ctcttttttg
      901 tgtgattggc gccttcgcct gtctgatgtt gtgccagacg caattcaagg ttatttgtac
      961 tgcctgtata atagctacca tcggcacact gcaatatgta catatagcct ttcatttgct
     1021 acaataaaat atcatttttg ttatgtttca ttatctcatt tcagctcttt tattttttgg
     1081 aggtgttctt ctgattatct ttccgcttca gccctttgta ttccttatca atgttggctt
     1141 ccagttcttc aatacttgga aggtcggttt tgagaacttt cgggattccc tttttcaaca
     1201 agtattccga aacgccaata ggtgttttga tgccctgcaa agaatacctg ataaccaatt
     1261 cattaggggt tttgcataac agtattccaa tggtggcatc gtctttattg ctttttatct
     1321 gagcatcaac ggtattgata tagaaattaa gtttaccggc atattccggt ttaaaatcgc
     1381 caattttaag ctcgaatatc acaaaacgat ggaggcgata attgtaaaaa agaaggtcga
     1441 ggaaatattc atcatcctct actgtcaggt caaactgatt gccaacatag gcgaagccct
     1501 tacctaattc gagcatgaac tttttcagat gctgaatgag gcctttttcc agttctcttt
     1561 cttttatttc ttcaccaaaa ccgatgaaat caaatatgta agggtttttc aggctctgct
     1621 gcaccagatc agattggagt tgcggcaggg tttgtctgaa attactgatg gcttttccct
     1681 gcctttcata aaggccgctt tctatttgtt ccaccagaat actccggctc cacccgtttt
     1741 cgacacactt ttgcatataa aacaactgct gttcccgtgt cttaacttta tccagcagta
     1801 cctgatgatg tccccatggt atttgtgcag caagttgctg cacaaatgaa tcagtatttc
     1861 cattgccgga ttgtgcagca gcttgctgca caattgttgt tttagctgta ttgagtattg
     1921 gtgcatcagg tggaagggca aatgaactaa attcaggata tgcttcggca aatgccctca
     1981 tgtatttgag gtttcgtacc gaaaggcctt taaaatccgg aaattcaatc tttaaatcta
     2041 ccgcaagccg gtcgatgatc tttgcacccc agcccgcttt tttctgttgt tctaaaatgg
     2101 cttttcctat ttcccaataa attttgagta gctcgtagtt tacggttatt acagccctgt
     2161 aacgtgtatg cctgattttt tctttcagca ggtcgagtac ttcaaaataa tttttttcag
     2221 gcaatttatt catatttgga tctttttcag gttttccata attgttttat tcaataccga
     2281 ttcttctgct atttgctttt caaactcggc tttcagcgaa ttaaaccttt caggaaaatt
     2341 aaaatcatct tcttcatccg gcaaaccgat gtatcggccc ggtgtaagca cgtaattctt
     2401 ttcacgtatt tcatcaatag taaccgattt gcaatagccc ggtatatcct ggtgccctat
     2461 gcttgtcgaa gtgcgccagt tgtggtaggt ggatgctacc ttgcttatat cttcatcggt
     2521 aaattcacgg ctgcggcggt taaccatgaa accgaggtta cgggcatcaa taaacaaaac
     2581 ttcgtttgta cggttgcgga atttgccatt tgctttgtta cgtgtcacaa accacagaca
     2641 agccggaatg ccggtattaa aaaataattt gtcgggcagg ttcacaatgc aatctatcaa
     2701 tctttcctca atcaggtttt tgcgtatctc tccctcgccg gaggtattgg ttgtaagcga
     2761 tccttttgaa agcacaaaac ctgcaatgcc gcttggcgca aggtggtaaa taaaatgctg
     2821 tatccaggca tagttggcat ttccggcagg cggcaggccg tatttccagc gtccgtcttt
     2881 ttgcagcagt tcgccgctcc agtcgctgtc gttgaaagga ggattggcaa gtataaaatc
     2941 tgctttcagg tcggggtgta cgtccctgag aaaactgcct tcattattcc atttaatatt
     3001 ggagccgtca atacctcgta tggcaaggtt catgcggcac aggcggtaag tggtctggtt
     3061 gctttcctgt ccgaa
//
LOCUS       DAOSFP010000287         3074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30021_length_3074_cov_3.613779, whole genome shotgun sequence.
ACCESSION   DAOSFP010000287 DAOSFP010000000
VERSION     DAOSFP010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30021_length_3074_cov_3.613779"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLI16_08270"
     CDS             complement(<1..215)
                     /locus_tag="PLI16_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84593.1"
                     /translation="MEQQLFKYPYYRANQFLTSQDLNESLSYVEEQERLTRIKMIGSG
                     ILSGLNFDNVLNAGDTLTEVVIKSGFG"
     gene            complement(219..2843)
                     /locus_tag="PLI16_08275"
     CDS             complement(219..2843)
                     /locus_tag="PLI16_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84594.1"
                     /translation="MLDSRDYIIEKNPVRPDALNYQVLRSYGLGYIQKLSSKLWTDHN
                     IHDPGITILEMLCYALTDLGYRTSFDMQDILTPAGKKGPEMKHAFHPAKKILTSHPVT
                     INDYRKFILDRVPGVRNVWFEIQDQKVYCPAVGFDATKKENLFVNPVSPGALKLKGLY
                     HVKVELEDYGIISQAHKNFLEKLKKYKENPNTDVGRTNYKKCYKNYIHSLLMSCRNIC
                     EDFEKVSILDDYFIGICADIELKPQAKDQHVLAEIYRKLYEYINPSIRFYTIQQLLDK
                     GRSIEQIFQGPVAERGFIDYEELMTFDRKTVLHISDLINSIMDIEGVLNIRSIQFNTA
                     KDNDNKNLKNKSCLHLADCADPEKYSFRFRFDIDFQSDNKLNKIIFRKGMIYYQARPT
                     KDYKITEVIDYAEKSADFENDLPEPSGTNRELDNYISLQDEFPKAYMTGREGIPNSAG
                     ELRKAQRLQLKAYLLFFDQLLADYLAQLDSVKDMLSWRDVDAEETYNYKKLTNEIFDL
                     DKILSEELLADYDKYQAVIEHVKVEKNRRNRLLDHLLARFNEKFVDYTVFKFVQNAEG
                     SSYDNYANNELIHDKKDMLKHYPAMSGNRSHAIDYTHPLSAKNFAMPEFRISKALGVD
                     ADDSNRLLAPEVVDNNPAKMIFENNSAQGFDKTFGLHIYEHILFRPLFADAAGPKHKF
                     LKLYYGDAGTASAKGIITDPYSMKATVVVPGWLNISARMEFRSFVEQKIRMEMPAHVA
                     LKICWIDPRQMWEMETKFKKFVDSLNDLFIPANTTVEAVITAYRTALNEVVAALSSLN
                     NMYPPSALDELLDFVTGNAKHTPVILDNTALAGGNDRNWAFDDENAAPEPVPKKKTVK
                     KKKTTVPKKVNSTNKKPK"
     gene            complement(2837..>3074)
                     /locus_tag="PLI16_08280"
     CDS             complement(2837..>3074)
                     /locus_tag="PLI16_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84595.1"
                     /translation="KAFIAPWMNNRVIIPAFSGVLYRSQIINFIEERPYVDYITAFDV
                     RKNGVSCSEAIRGSEENILLTSSETHHISTEATC"
BASE COUNT          770 a          672 c          679 g          953 t
ORIGIN      
        1 atgccaaaac cactttttat gacaacctcc gtcagtgtgt cgcctgcatt caggacattg
       61 tcgaaattca ggccgctgag aatcccgcta ccaatcattt ttatacgggt cagtcgttcc
      121 tgttcctcaa cataagataa tgactcattg aggtcctgac tcgtaagaaa ttggtttgcc
      181 cggtaatagg ggtatttgaa tagttgctgt tccatggctt atttgggttt tttgttcgtc
      241 gaattaactt ttttggggac cgttgttttt ttctttttca cggttttttt cttgggcaca
      301 ggttccggcg ctgcattttc atcatcaaaa gcccagttgc ggtcgttgcc gcctgcaagt
      361 gccgtattat ctaaaatcac aggagtatgt ttggcattgc cggtcacaaa atcaagcagc
      421 tcgtcgaggg ccgatggcgg gtacatattg tttagcgaag acaaggcggc caccacctcg
      481 ttcaaggctg tccggtaggc agtgatgacc gcttccacag tagtgttggc ggggatgaaa
      541 aggtcattga ggctgtcaac aaatttttta aattttgttt ccatttccca catttggcgg
      601 gggtcgatcc aacatatttt taaggccaca tgggcgggca tttccatacg gatcttttgt
      661 tccacaaaac tcctgaactc cattcttgcc gagatattga gccatcccgg aacaaccacg
      721 gtagctttca tggaataggg atcggtaata atacccttgg ctgaggcggt tcccgcatca
      781 ccataataca gtttcagaaa cttatgctta ggacctgccg catcggcaaa caaaggcctg
      841 aaaagaatat gctcataaat atgcagtccg aaagttttat caaacccctg cgcactgttg
      901 ttttcaaaaa tcatttttgc cgggttgttg tccaccactt cgggcgccag caggcggttg
      961 ctatcgtcag catccacacc cagtgctttg ctgatgcgaa attctggcat agcaaagttt
     1021 ttagccgaca gcggatgggt ataatcaatg gcatgagaac ggttgccgct catagccggg
     1081 taatgtttta gcatatcttt tttgtcgtgg ataagctcgt tgttggcata attgtcgtac
     1141 gacgagcctt cggcattctg aacaaattta aagacggtat agtcaacaaa tttttcattg
     1201 aaccgcgcca gcaggtggtc gagaaggcgg tttctgcggt ttttctccac ttttacatgt
     1261 tcaatcaccg cttggtattt gtcataatca gccaacagtt cttccgacag aattttatcc
     1321 aggtcaaaaa tctcgttggt cagtttttta taattatagg tttcttcggc atcgacatca
     1381 cgccacgaaa gcatatcttt aacagaatcg agctgtgcca ggtaatcggc cagcagctga
     1441 tcgaaaaaca aaagataagc cttcaactgc aggcgttgtg cttttcgcaa ttcgccggcg
     1501 ctgttgggga tgccttccct gccggtcatg taggccttgg gaaactcgtc ctgaagcgag
     1561 atataattat ccagttcgcg gttagttcct gatggctccg gcaaatcatt ttcaaaatcc
     1621 gctgattttt cagcataatc tattacttcg gtgattttat aatccttagt tggccgggcc
     1681 tgataataaa tcatcccctt gcgaaaaatg attttattta acttattgtc cgattgaaag
     1741 tcaatatcaa aacgaaaacg aaatgagtat ttctccgggt cggcgcaatc agccagatgc
     1801 aggcatgatt tatttttaag gtttttgtta tcgttgtctt tggccgtatt gaactgtatg
     1861 cttctgatat ttaatacgcc ttcaatatcc atgatgctat ttatcaggtc agaaatatga
     1921 agcaccgttt tcctgtcaaa agtcatcagt tcttcataat caataaaacc ccgttcggca
     1981 acaggcccct ggaaaatttg ttcgatactt cttcccttat cgagcaactg ttgtatggtg
     2041 taaaatctga tggaggggtt tatatattcg tacagtttgc ggtatatctc tgccagcaca
     2101 tgctgatctt tagcctgggg tttcaactct atatcggcac aaatgccaat aaaataatca
     2161 tccaggatgc ttactttttc aaagtcttcg caaatgttgc ggcaactcat cagcaagctg
     2221 tgaatatagt ttttataaca ctttttgtaa ttagtccggc ccacatccgt attgggattt
     2281 tctttatatt tttttagttt ttcaagaaag tttttatgcg cctggcttat tattccgtaa
     2341 tcttcaagtt ccacttttac gtgataaagt cctttgagct tcagcgcgcc agggcttacc
     2401 gggttaacaa acaaattctc ttttttggtg gcatcaaagc cgacggcggg acagtatacc
     2461 ttctggtcct gaatttcaaa ccacacattg cggactccgg gcaccctgtc gaggataaac
     2521 ttcctgtaat cgttgatagt aacgggatgc gaagtaagga ttttttttgc cggatgaaaa
     2581 gcatgtttca tttcggggcc tttttttcct gccggagtaa gaatatcctg catatcaaag
     2641 gatgtgcggt accccaggtc ggtaagggcg taacataaca tttcgaggat agtgattccc
     2701 gggtcgtgga tgttatggtc ggtccacagt ttgctgctca acttttgaat ataacccagg
     2761 ccgtagcttc tgagcacttg ataattaagc gcgtcgggcc tgacgggatt tttttcaatg
     2821 atataatctc ggctgtctaa catgttgcct ccgtgcttat atgatgggtt tctgatgagg
     2881 tcaataaaat attttcttcg gaaccgcgaa tagcttcact gcacgaaaca ccgtttttcc
     2941 tcacatcaaa agcggtaata taatcgacat agggccgctc ttcaataaaa ttgatgatct
     3001 gtgaacggta aagcactccc gaaaaggcgg gtatgatgac acggttgttc atccatgggg
     3061 caataaatgc tttc
//
LOCUS       DAOSFP010000288         3050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30347_length_3050_cov_3.764608, whole genome shotgun sequence.
ACCESSION   DAOSFP010000288 DAOSFP010000000
VERSION     DAOSFP010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30347_length_3050_cov_3.764608"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLI16_08285"
     CDS             complement(<1..264)
                     /locus_tag="PLI16_08285"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOH84596.1"
                     /translation="MENIQIVALIIFFITYVGIIFTRLPWINIDRPSAAFFGAVAMIL
                     FSVLSFEEAVLSIDFNTIALLLGMMIIISTLQLDGFFSFIARKT"
     gene            complement(266..2374)
                     /locus_tag="PLI16_08290"
     CDS             complement(266..2374)
                     /locus_tag="PLI16_08290"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOH84597.1"
                     /translation="MFWNKYPIARILLPFLSGICIAIGFELSFAIPVIVFALLAGLIS
                     FVVIFFQRKLSFRMRWLTGILFTTFFFLAGYQLTVIKTKQHDNTHYSNYLAAENYVLA
                     ELCEPVQIREKVCKCVVEIKEVKDSVQSCRTSGKAIIYLEKDSLSSKLKYGDEIFFRG
                     RFTEVSPPMNPGEYNYKNYLKYRQVEYSTYVMKARWKLIASGNGSVLKGFAYNLREKL
                     LQILKDNGLQGQEFAVVSALLVGYTDNLDPELIREYQGTGAVHILSVSGLHVGIIFIV
                     LNFLLQFFDKTRYGRLPKALLLLVFIWFYAVLTGMSPSVMRATAMFSFVVVGKAFRQP
                     PAIYNTIAASALVLLMFDPYMFMAVGFQLSYLAVLGIVALYPAISKVWIPKYWLLRQI
                     WSLVAVSLAAQLVTFPLCLLYFHQFPNYFLLTNIIATPLSGLVIYLGIAVLVFSFIPW
                     LAMMLAKALSYSLIFLNSSVSFIEGLPFSVTRSISVTFVEMLLVYAIILSLCVFIMRR
                     NNKAFIGAAAFLLLLVFSVTVRNFNALSQQAIIVYNINKHTAIDLIEGKTAFFICDSA
                     VMSDVKKQEFHLMNNRCRMMIEEVIPLPVGDGDITKKKENLFARKNLYCFAGKTLGIV
                     NGATYNTKKMKLDYMVVANNPDLTMEEMLNQYAPVFIIFDASNSAKNVAGWSNQCEML
                     GKSYYSTKNSGAWQCFMNNK"
     gene            complement(2378..>3050)
                     /locus_tag="PLI16_08295"
     CDS             complement(2378..>3050)
                     /locus_tag="PLI16_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOH84598.1"
                     /translation="AITCRTYALNNIKKHAKEGFNLCDSVHCQLYLGRCKTNEILAAT
                     FKTAGDVIVDKDNKMISAAFHANSGGQTVNSEDIWKIPTSYLKSVVDTFSLRMSGAVW
                     EEKILQANWLDYLSSRFNYPVEDPAKKNYALNFKQDKRALYFSDSIPLSAIRYDLGLK
                     STWFSVSPAGSNVVLRGKGYGHGVGLSQQGAIRMAQLGYNYLEIIKFYYKDVEVVNYD
                     ELNIK"
BASE COUNT          930 a          705 c          641 g          774 t
ORIGIN      
        1 ggttttgcgt gctatgaacg aaaaaaaacc gtcaagctgc agggtggata tgataatcat
       61 cataccgagc agcaaagcaa tagtattaaa atctatggac aaaacagcct cttcaaagct
      121 cagcacacta aacaatatca ttgccacagc accaaaaaat gccgccgatg gccggtcgat
      181 attaatccac ggcaggcggg taaaaatgat gcccacatag gttataaaaa aaatgataag
      241 ggcaacaatc tgaatatttt ccatattact tgttattcat aaaacactgc caggctccgg
      301 aatttttcgt ggaataatac gattttccga gcatttcgca ctggttggac catcctgcaa
      361 catttttggc tgaattggaa gcatcaaaaa taatgaaaac cggcgcatat tggttcagca
      421 tctcttccat ggttaaatcc ggattgttgg ccacaaccat ataatccagc ttcattttct
      481 ttgtattata agttgccccg tttacaatgc caagtgtctt accagcaaag cagtacaggt
      541 tcttccgggc aaataaattt tcctttttct ttgtgatatc accatcaccg acaggcagag
      601 ggatgacctc ctctatcatc atccggcaac ggttgttcat aaggtgaaac tcctgttttt
      661 tcacatcgct catcaccgca gaatcacaga taaaaaatgc cgtcttgccc tcgatgaggt
      721 caattgcagt atgtttgtta atattataaa ctatgattgc ctgctgcgac agcgcgttaa
      781 agttccgtac ggtaacggaa aaaaccagta gcaggagaaa agccgccgca cctataaagg
      841 ccttgttgtt tcgccgcatg ataaacacac aaagcgacag aataatggca tacaccagca
      901 acatttcaac aaaggttacc gatatggacc tggtaaccga aaaaggtagg ccttcaataa
      961 aagaaacaga gctgttaagg aaaatcagcg aataagacaa agcctttgcc agcatcatgg
     1021 cgagccaggg aataaaacta aataccagta cagctatgcc gaggtatata accaatcccg
     1081 acaagggtgt tgcgatgatg ttggtaagca ggaaatagtt gggaaactga tgaaaataca
     1141 gcagacacag aggaaaagta accagttgtg ctgccagcga aacagccacc agcgaccaaa
     1201 tctgtcgcag caaccagtat ttcggtatcc acaccttgct gatcgccggg tatagagcta
     1261 cgattcccaa tacagccagg taggacagtt gaaagcccac cgccatgaac atatatggat
     1321 cgaacatcaa caataccaat gccgaggccg cgatggtatt gtatattgca ggtggctggc
     1381 gaaaggcttt gcccacaact acaaaactaa acatagccgt ggccctcatc accgatggag
     1441 acatgccggt aagcaccgca taaaaccata taaaaaccag cagtaacagc gccttgggca
     1501 ggcgtccata tctggtcttg tcgaaaaact gcaataaaaa attcagcaca ataaaaatga
     1561 tgcccacatg aaggcctgag acactcagaa tgtgtacggc gccggtaccc tggtattcgc
     1621 ggatcagttc gggatcgagg ttatcggtat atccaaccag caaggccgac accacagcaa
     1681 attcctgtcc ctgcaggcca ttatccttca atatttgaag tagtttttcg cggaggttgt
     1741 acgcaaatcc cttgagaaca cttccattac ccgaagcaat caatttccac cgggctttca
     1801 tgacataagt gctgtattcc acctgacggt atttcaggta gtttttataa ttgtattcac
     1861 cagggttcat gggcggagac acctccgtaa aacggcccct gaaaaaaatc tcatcgccat
     1921 atttcagttt tgatgacaag gaatcttttt ccaggtaaat aattgctttg ccgcttgtac
     1981 ggcaggattg cacactatct tttacctctt ttatttccac cacacatttg catactttct
     2041 ctcttatctg cacaggctcg cacaattccg caaggacata gttctcagcg gcaaggtagt
     2101 tactgtaatg ggtgttgtcg tgttgtttag tttttatcac tgtcagttga taaccagcca
     2161 ggaaaaaaaa cgtggtaaac agtatcccgg taagccagcg catacgaaaa ctcagtttcc
     2221 tctgaaaaaa aatcaccaca aaagaaatca gacctgctag gagagcaaaa acaatgacag
     2281 gtatggcaaa gctaagttca aagcctatag cgatgcaaat tccggataaa aaaggtaaga
     2341 gaatgcgtgc aattgggtat ttattccaaa acatgtacta cttgatattc agttcgtcat
     2401 aattgaccac ttccacatcc ttataataga acttgatgat ttccaggtag ttgtatccga
     2461 gctgagccat tctgatagca ccttgttggc tcagaccgac gccgtgtcca tagccctttc
     2521 ccctgagtac tacattagac cccgcaggag ataccgaaaa ccaggtggat ttcaagccaa
     2581 gatcatatct tattgccgac aagggtatgc tgtcggagaa atacagcgcg cgtttgtcct
     2641 gtttaaagtt cagggcatag ttttttttgg ccgggtcttc caccggataa ttaaagcggc
     2701 tggacaaata gtcgagccag ttggcttgca ggattttctc ttcccaaaca gctccggaca
     2761 tccgcaatga aaaagtatcc accacggatt tcaggtaaga ggtgggtatt ttccaaatat
     2821 cttcagaatt gacggtctgc cctcccgaat tggcatgaaa tgcagccgat atcattttat
     2881 tatccttgtc cacgataaca tcgccggcag ttttaaaggt agctgccagt atttcgttgg
     2941 ttttgcaacg tccaagatat agctgacaat gaacactatc gcacagatta aacccctcct
     3001 tggcatgttt cttaatgttg ttgagtgcat aggtacgaca ggtaatggcc
//
LOCUS       DAOSFP010000289         3046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30403_length_3046_cov_3.645269, whole genome shotgun sequence.
ACCESSION   DAOSFP010000289 DAOSFP010000000
VERSION     DAOSFP010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30403_length_3046_cov_3.645269"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..153)
                     /locus_tag="PLI16_08300"
                     /pseudo
     CDS             complement(1..153)
                     /locus_tag="PLI16_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
     gene            complement(202..1254)
                     /locus_tag="PLI16_08305"
     CDS             complement(202..1254)
                     /locus_tag="PLI16_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HOH84599.1"
                     /translation="MDPVMIYWIVFIGLFITVFIIDLYVTDHRKGALTVKTSLKWTGL
                     WISIALLFGVSLYFFFPQNPDSAVKTAPVMMTKFIAGYLTEYSLSVDNLFVFIMIFSM
                     MAVSQKNQPRLLKLGILISIALRILFILVGMELVEKFHWIIYVFGVILIWTAYKMAFS
                     KEDDQVDPSKNILFKGASKLFPVDPDKDPHKFFNKINGKWHITSIFLVLLVIGSTDVL
                     FAVDSIPAIIGVIKEGATNILTPEEENFIAITSNVFAVMGLISLFFALKGIIGLFRFL
                     KHGVSFILLFIGAKMLLSAIPAVMGFFSNHSWVSLVVIIATLTLSILLSMLISEKKEI
                     DQLKDEVDDLKENILN"
     gene            complement(1267..1707)
                     /locus_tag="PLI16_08310"
     CDS             complement(1267..1707)
                     /locus_tag="PLI16_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOH84600.1"
                     /translation="MGKYKFKINEKQYDVEIANIDDNIADVVVNGKSYKVEVEKSLQT
                     TKTPKLVRTMAVPSTDGSPQTAKTSSPSAPKGSGVVKSPLPGLILNIYVNVGDPVSIG
                     QKIITLEAMKMENNINSDKEGRVTSIKVKKGDSVMEGDVLIEIG"
     gene            complement(1735..1980)
                     /locus_tag="PLI16_08315"
     CDS             complement(1735..1980)
                     /locus_tag="PLI16_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84601.1"
                     /translation="MAVFKKKKIEPPVVEVVPEEINPDEVSAVIAMALDLHLKDIHDY
                     EKTVLTIQKVMRPYSPWSSKFYGLRQMPLHIPRQRLK"
     gene            complement(1992..>3046)
                     /locus_tag="PLI16_08320"
     CDS             complement(1992..>3046)
                     /locus_tag="PLI16_08320"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOH84602.1"
                     /translation="AGGAVYSPALTDFIMMTKKSSYMFVTGPKVVKTVTGEVVTDEEL
                     GGAMVHASKSGVNHFVAEDEQEGMQLIRKLLSYMPQNNLEDPPLATCEDPIDRLEDFL
                     NDIVPDNPNKPYDVKDIIHAIVDYNEFLEVMRHFAPNIIIGFARFNGMSVGVIANQPN
                     YLAGVLDINASRKAARFVRFCDAFNIPLVTLVDVPGFLPGTTQEYGGIIIHGAKLLYA
                     YGEATVPKVTVTLRKSYGGAHDVMSSKQLRGDINYAWPSAEIAVMGPKGAIEVLYQKE
                     LKEITDEAQKAAFIAEKENEYKDKFANPYQAAKFGYIDDVIEPRNTRFRIIRALQSLA
                     TKKDQNPPKKHSNLPL"
BASE COUNT          902 a          645 c          609 g          890 t
ORIGIN      
        1 aatttgtgta agtgcggaag tcaaggggat gttcactaac actgccccaa aacccattgg
       61 caacagtaaa tttggttcgt attcctttgc aatggcaaga taaatgagtg ttaaacccac
      121 ggcaatcatt accagatttt gccaggtaat gctataaata accggcaaca aactttgtaa
      181 atccataatt cttaatattt tctaatttaa tatattttct tttaaatcat ccacttcatc
      241 ttttagctgg tcaatttctt tcttttcaga aatcaacatg gacagcagta tgctaagagt
      301 taacgtggca ataatgacca ccagagacac ccaggaatga ttggagaaaa atcccattac
      361 tgccgggatg gccgacaaca gcatttttgc accaataaat aacaggatga aactcacgcc
      421 gtgttttaaa aagcggaaca ggccgataat ccctttaagg gcaaaaaata aggaaatcaa
      481 acccataacg gcaaacacat tggaagtgat ggcaatgaaa ttttcttctt caggagtcag
      541 gatgttggta gctccttctt tgataacccc aatgatggcc gggatagaat ccacagcaaa
      601 caacacgtcg gtggatccga ttaccagcag caccaaaaat atggaagtaa tgtgccattt
      661 cccgtttatt ttattaaaaa acttgtgagg atctttgtcc ggatcaacag ggaaaagctt
      721 agaggctcct ttgaaaagaa tatttttaga ggggtccacc tgatcatctt ctttcgaaaa
      781 agccatttta taggccgtcc agatcagaat cacaccaaac acataaataa tccaatgaaa
      841 cttttccacc agttccatac caaccaaaat gaataaaatc ctgagagcga tagaaatcaa
      901 tatcccaagt tttaataagc ggggctggtt tttctggcta acggccatca tcgaaaaaat
      961 catgataaat acaaaaaggt tatccaccga cagggagtac tcggtaagat aacccgcaat
     1021 gaattttgtc atcataacag gggctgtttt caccgcgctg tcaggatttt gcggaaagaa
     1081 gaaataaagg gacactccga aaagcaacgc gatgctaatc cataggcctg tccattttaa
     1141 ggaggtttta actgtaagcg cacctttacg gtggtcggta acatataagt caataataaa
     1201 aacggtaatg aatagtccaa taaaaactat ccaataaatc ataactggat ccatgtcaaa
     1261 aaatttttat ccgatttcaa ttaatacatc accttccata acggagtcac cttttttcac
     1321 tttaatgctg gttactctac cttctttatc ggagttgata ttgttttcca ttttcatggc
     1381 ttcaagggta ataattttct ggccgataga aacgggatcg ccaacattaa catagatatt
     1441 caaaattaaa cccggcaacg gagattttac cacgccactg cctttggggg ccgaaggcga
     1501 agaggttttt gccgtttgcg gcgaaccatc cgtggaagga acggccattg ttctgaccag
     1561 cttgggggtt ttggtggttt gcaacgactt ttctacttcc actttatagg actttccgtt
     1621 aacaaccaca tctgcaatat tatcatcgat gttcgctatt tcaacatcgt attgtttttc
     1681 attaatttta aatttatact tacccatatt tttgcttttt tggattattg acaattattt
     1741 taatctttga cggggaatat gcaaaggcat ctgcctgagt ccgtaaaact ttgaactcca
     1801 gggagaataa ggtcgcatga ctttctgaat tgtcaacacg gttttttcat aatcatgtat
     1861 atctttcaga tgtaaatcaa gtgccattgc aataaccgcc gacacctcat cgggattaat
     1921 ttcttccggt acaacttcaa ccaccggtgg ttctattttt ttctttttaa aaactgccat
     1981 gacccatcag attaaagtgg taaattggaa tgttttttag gaggattctg gtcttttttg
     2041 gttgccagcg actgtaaggc gcggatgatt ctgaagcggg tattccgtgg ttcaattaca
     2101 tcatcaatgt atccgaattt tgcagcctgg taaggattag cgaatttgtc cttgtattcg
     2161 ttttcctttt cggcaatgaa agctgctttt tgagcttcat cggttatttc cttcaattct
     2221 ttctgataca aaacttcgat agcgcctttg ggtcccatga cggcgatttc ggctgaaggc
     2281 catgcgtagt tgatatctcc gcgaagctgt tttgagctca ttacatcgtg tgcgccaccg
     2341 tatgatttac gaagggtaac ggtaacttta ggcacagttg cttctccata cgcatacaga
     2401 agttttgcgc catggataat aataccgcca tattcctgcg tggtgccggg taaaaatccc
     2461 ggaacatcca ccagggtaac caggggaata ttaaatgcat cacagaaacg gacaaaacgg
     2521 gcagctttgc gtgaagcatt gatatcgagc acaccggcga gatagttggg ctgattggct
     2581 ataacgccaa cagacatccc gttgaaacgt gcaaaaccaa tgataatatt cggtgcaaaa
     2641 tgcctcatca cctccaaaaa ttcattataa tccacgatgg cgtgaatgat atcttttaca
     2701 tcataaggtt tgttgggatt atccggaaca atgtcattaa gaaaatcttc gagccggtcg
     2761 ataggatctt cgcaggttgc caatggagga tcttccagat tattctgggg catatagctc
     2821 agaagtttgc gtatcagctg catgccttcc tgttcgtctt ctgccacaaa atgattcacg
     2881 cctgattttg aagcatgtac catagcgcca cccagttctt catcagtgac cacttcgccg
     2941 gtaaccgttt ttactacctt gggaccggta acaaacatat aactgctctt tttggtcatc
     3001 atgataaaat cggtaagagc cggagaataa acggcgccgc cggcac
//
LOCUS       DAOSFP010000290         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30545_length_3035_cov_3.900000, whole genome shotgun sequence.
ACCESSION   DAOSFP010000290 DAOSFP010000000
VERSION     DAOSFP010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30545_length_3035_cov_3.900000"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="PLI16_08325"
     CDS             <1..532
                     /locus_tag="PLI16_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HOH84603.1"
                     /translation="GTKANFETVIGDNTLIMAYVHVAHDCIVGNNVIIANSAGLAGHI
                     KIDDWAIIGGMVAIHQFVSIGAHSLISGGSLVGKDVPPYCKAAREPLAYSGINSIGLR
                     RRGFSSEKIKEIQDIYRIIFVNKFNVSQAINFIEAEIPATPERDEIILFIQNSKRGIM
                     KGYKRNGCNNTKKESI"
     gene            673..1233
                     /locus_tag="PLI16_08330"
     CDS             673..1233
                     /locus_tag="PLI16_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOH84604.1"
                     /translation="MPARIRLQRRGKKGQAYYHIVIADGRAPRDGKFIESIGTYNPLT
                     IPAQIELDIDKAVTWLRNGATPSDTAKAILSYKGVLYKNHLLKGVAKGAMTAEQAEAK
                     FNIWMQEKQAKIQAKVNNQLLSKKDAAKKRHEAEAKVNLAISEAVAKKRAKLQEQEMA
                     AAAPAEEANNEAEAPAENTETGEKPE"
     gene            1250..1777
                     /gene="rimM"
                     /locus_tag="PLI16_08335"
     CDS             1250..1777
                     /gene="rimM"
                     /locus_tag="PLI16_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOH84605.1"
                     /translation="MEKEQFSYVGKIIKTPGYKGEILAQIEAGFIDKFKKTEFVFVEI
                     QHEQVPFLILSRENKYHHVFLFSLDDIDTVEKAQKLTGCRLFIAEQAKKKKISGDFEL
                     KELIGFEVQDKKSGKIGKIACFLELPQQQIMQIFKDKKEILIPFNEAIVLAIDVAHKT
                     VTIRAPEGLIDHYLK"
     gene            1774..2484
                     /locus_tag="PLI16_08340"
     CDS             1774..2484
                     /locus_tag="PLI16_08340"
                     /inference="COORDINATES: protein motif:HMM:NF017755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOH84606.1"
                     /translation="MRQPFRFKQFSVSDDLCGMKLTGDAVLLGAIASPGTSGKILDIG
                     TGCGILSLMMAQKSNALIDAVEADANAAMQASRNFAQSPWNNRIEIHQMAIQEYAEKC
                     QKKYDCIISNPPYFQNKLKSTALNKSDARHNSNLDFARLSHCVSRLLSETGTFWAIMS
                     VSEKNNFLKAFLTRGLYCHSTIFIYDKPGREPKRLISCFSFTVPESVPEQTLILKNPD
                     NSPSKAFIALTKDFYPGF"
     gene            2531..2878
                     /locus_tag="PLI16_08345"
     CDS             2531..2878
                     /locus_tag="PLI16_08345"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOH84607.1"
                     /translation="MKKIMSFLLVITAIVLACQPLYAQKVYSCDSKYDADVKVYVADS
                     KYDADLVVYKCSSQYDAGDNDGVWFFVDSKYDAKKKIYFVDSKYDADLIIYFTDSKYD
                     AGWRNNSKKHLMY"
BASE COUNT          991 a          712 c          614 g          718 t
ORIGIN      
        1 aggcaccaag gccaactttg aaaccgttat cggcgacaac acccttatca tggcttacgt
       61 tcatgtggca cacgactgca ttgtgggcaa caacgtgatt attgccaatt cggcaggcct
      121 ggccggccat atcaaaatcg acgactgggc tatcattggc ggcatggtgg ccatacatca
      181 gtttgtcagc attggcgctc actcactcat atccggggga tccctggtag gaaaagacgt
      241 gcccccctat tgcaaagccg cccgcgaacc ccttgcctat tcaggaataa actccatcgg
      301 cttgcgccgc agaggatttt cctccgaaaa aataaaagaa atacaggaca tctaccgcat
      361 catttttgtc aataaattca atgtatccca ggccataaac ttcatagaag ccgaaatccc
      421 cgccacaccc gaaagagatg aaatcatttt gtttatccaa aactcaaaac gcggcatcat
      481 gaaaggctac aaacgcaatg gatgcaacaa caccaaaaaa gagagcatct aacggataat
      541 caatcttttg catcagcttt tgcctggatt tacaggaaat acaaaaaaaa tatttcatta
      601 ttcggaaata tttacgatct ttgcacccca atttcaatta ataaaaaaca ttaacaatta
      661 acagttaaaa ctatgccagc aagaatcaga ttacaacgtc gtggtaaaaa aggtcaagcc
      721 tattaccata ttgtaattgc ggatggtcgt gcaccacgtg atggaaaatt cattgagagc
      781 attggcacct acaaccctct tacaatacca gcccaaatcg aactcgatat cgacaaagct
      841 gttacctggc tccgcaacgg agcaacccct tcggacaccg ccaaagccat tttgtcttac
      901 aaaggcgtcc tgtacaaaaa ccacctgctg aaaggtgtcg ccaaaggcgc catgacagca
      961 gaacaggccg aagccaaatt caatatctgg atgcaggaaa aacaagccaa aatccaggct
     1021 aaggtcaaca accaactcct cagcaaaaaa gatgctgcca aaaaacgtca cgaagccgaa
     1081 gcaaaagtaa acctcgccat ctcggaagct gtggcaaaga aacgcgccaa actgcaggaa
     1141 caggaaatgg ccgcagcagc ccccgctgaa gaagctaaca acgaagcgga agcccccgcc
     1201 gaaaataccg aaacaggaga aaaaccagaa taaaacctct tgcccggtaa tggaaaaaga
     1261 gcaattttcc tatgtgggta aaataatcaa aactcccggt tataaaggcg aaattctggc
     1321 ccaaatcgaa gcgggtttta ttgataaatt taaaaaaacg gaatttgttt ttgttgaaat
     1381 acaacacgaa caggttccgt ttcttatttt atcgcgcgaa aacaaatacc accacgtttt
     1441 tttattttca ctcgacgaca tcgacaccgt cgaaaaagca caaaaactta ctggttgccg
     1501 ccttttcatt gccgaacagg ctaagaaaaa aaaaatatcc ggcgattttg agctgaaaga
     1561 gttgataggt tttgaggtgc aggacaaaaa atcggggaaa ataggaaaaa ttgcctgctt
     1621 cctcgaactc ccccaacaac agataatgca gattttcaaa gacaaaaaag aaattctgat
     1681 accctttaat gaagctattg ttctggccat cgatgtggcg cacaaaacgg ttactatccg
     1741 ggctccggag ggactgatag atcattacct gaaatgaggc agcctttccg gttcaaacaa
     1801 ttcagcgttt cagacgactt gtgcggcatg aaactcaccg gcgacgctgt tttgcttggc
     1861 gccatagcct cccccggcac atcaggaaaa attttagata taggcaccgg ctgcggcatc
     1921 ctgtctctga tgatggcaca aaaaagcaac gccctgattg atgctgtgga agccgatgcg
     1981 aatgccgcca tgcaggcctc acgcaatttc gcgcaaagcc catggaacaa ccgaatagag
     2041 attcatcaga tggccatcca ggaatatgca gaaaaatgcc aaaaaaaata cgattgcatc
     2101 atcagtaatc caccctactt tcagaataaa ctgaaaagca cagcgctaaa caaaagcgat
     2161 gcccggcaca acagcaacct ggattttgcc cgactgagcc attgtgtaag ccgcctgctc
     2221 tcggaaacag gcaccttttg ggccattatg tcggtttcgg aaaaaaacaa ctttctgaaa
     2281 gcctttttga ccagaggatt gtactgccat tctacaattt tcatctatga caaaccaggc
     2341 agggaaccca aaagactaat ttcgtgtttt tctttcactg tgcctgaatc cgttccggaa
     2401 caaaccctga tactcaaaaa ccctgacaac agcccgtcaa aggcttttat agcgctgacc
     2461 aaagattttt acccggggtt ttgaatttgg aatgaaaatt ggtaaatttg caaggatcaa
     2521 aaccatgaaa atgaaaaaaa taatgtcttt cctgctggtt attactgcca tagttttagc
     2581 gtgccagcca ctttatgccc aaaaagtata ttcatgcgac agcaaatacg atgctgatgt
     2641 aaaagtttac gtggccgaca gcaaatacga cgccgacctg gtggtgtata aatgctcaag
     2701 ccagtacgat gccggagata acgacggcgt ttggtttttt gtggatagca aatacgatgc
     2761 gaaaaagaaa atatactttg tggacagcaa gtatgatgct gacctgatta tttatttcac
     2821 cgacagcaag tacgacgccg gctggagaaa taattcgaag aaacacctga tgtattaaac
     2881 actcccattg tactttttgg agagtacatt tatttcttac tttgccaatt catatgtaaa
     2941 gaactcatga gcaggtatta ttttgatggt gttcttatca taaactaagg tatccttttg
     3001 actttttgtg atgatataac cttctttgaa cttaa
//
LOCUS       DAOSFP010000291         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30571_length_3034_cov_2.239678, whole genome shotgun sequence.
ACCESSION   DAOSFP010000291 DAOSFP010000000
VERSION     DAOSFP010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30571_length_3034_cov_2.239678"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLI16_08350"
     CDS             <1..453
                     /locus_tag="PLI16_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84608.1"
                     /translation="AYGHNTLAQTGWYRGGLDYRRLPNQYGVQNLFCKNNLIFLVEYF
                     CQTSVLTNNDNLSSIDSIPVMDNEPLLYPNPAAGKFIIQLCGNNNKIVDITLTNIDGA
                     LVYKTRKTLKGNGMDSIEINVEGLPAGNYFCSIVAGNSIVSKKITLID"
     gene            468..947
                     /locus_tag="PLI16_08355"
     CDS             468..947
                     /locus_tag="PLI16_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOH84609.1"
                     /translation="MMMKITLRKAGKNDIPEILALIRELATYERAPQEVTVTAEDLEK
                     DGFGDNPAFEVLLAFNDEQLLGMAFYYFSYSTWKGKCLYLEDIIVKEQFRGNKAGKLL
                     FEAVIKKARETGAKRMHWQVLEWNEPAINFYKKYGAVLDETWTNGKFTEEQLAAFKL"
     gene            1006..1326
                     /locus_tag="PLI16_08360"
     CDS             1006..1326
                     /locus_tag="PLI16_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOH84610.1"
                     /translation="MKKVFLKIWSVLKNYYVLATVVFIVWVGFLDTDNLMSQISQRKE
                     MNGLLEQKKFYTEEIQRMKELSQSLKTDKEAMERYGRENYQMKKKSENIFLIVPPKSD
                     TKEK"
     gene            1736..2833
                     /locus_tag="PLI16_08365"
     CDS             1736..2833
                     /locus_tag="PLI16_08365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH84611.1"
                     /translation="MKSGNKVILILIMIVACALRFYDFFDIPYTHDEFSALFRTRFNT
                     FADLIDKGVKVDTLPAGIQIFLFYWIKIFGETEWIVKLPFLLFGLGAVYFVYVVGKKW
                     YNETVALVAAAFLASLQYTVFYSQVARPYISGLFFSLLMVNALTNLIKTPEKRFYSNM
                     LIFVFAAAACAYNHHFSLVFAAIAGVSGLFMIRKDFLIKYLAACFVSVLLYLPHIKIV
                     LAQLQMGGNENWLGAFDWSFLYNYFRYIFQFSIISYIMVGFLIAFEILGYKKSNAKPG
                     PSLLFLSWFVLPLLVGVLYSVCVNNVMQYSVLIFSFPYLLFLLFGHIPKQKTIINLLL
                     VFLILCVNVSALVFERHHYSSLYESAYLHIL"
     gene            2849..>3034
                     /locus_tag="PLI16_08370"
     CDS             2849..>3034
                     /locus_tag="PLI16_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84612.1"
                     /translation="MKNYKNVVTLIDTDKEISDYYLQKETYTAHYTFVDTTFTRKRFV
                     SFLKESSRHSPYFYYGCL"
BASE COUNT          840 a          550 c          670 g          974 t
ORIGIN      
        1 gcttacggcc ataataccct ggcacaaaca ggctggtacc gcggaggcct tgattacaga
       61 aggcttccaa accaatatgg cgtgcaaaac ctgttttgta aaaacaactt gattttcctg
      121 gttgaatatt tttgtcagac ctcggtactt actaataatg ataatttgag ttccattgat
      181 tccataccag ttatggataa tgagccgttg ctatatccca atcctgcagc cggaaagttt
      241 atcattcaac tatgtggcaa caataacaaa atagttgata ttacccttac aaatattgat
      301 ggggcgttgg tttacaagac caggaaaact ttaaaaggaa acggtatgga ttcgatagaa
      361 ataaatgttg aaggtcttcc ggcgggcaat tatttttgca gtattgttgc aggaaacagc
      421 atagtttcga agaagataac cctaattgat taaaaatata atccgatatg atgatgaaaa
      481 ttacattgcg taaagccggc aagaatgata tccctgaaat tctcgcgctg atcagggaac
      541 tggcaacata cgaacgcgcc ccgcaggagg ttaccgttac ggcagaagat ctggaaaagg
      601 atggatttgg cgacaacccg gcttttgaag ttctgcttgc tttcaatgat gagcagttgc
      661 tcggcatggc tttttattat ttcagctatt ccacatggaa aggcaaatgc ctgtatcttg
      721 aagatatcat cgtaaaagaa cagtttcgcg gaaacaaggc cggaaagttg ttgtttgaag
      781 ccgtaattaa aaaggcccgg gagacgggag ctaaacgcat gcattggcag gtactggagt
      841 ggaacgagcc ggccataaat ttttataaga agtatggcgc cgtgttagat gaaacctgga
      901 caaacggtaa gtttaccgaa gaacaattag cggcttttaa gctgtgaaaa tttttttatt
      961 ttaaatgacg gataatacca atgcattatc tatatttgtg ctatcatgaa aaaggttttt
     1021 ttaaagatct ggagtgtcct gaaaaactat tacgtccttg ccaccgtcgt atttatcgtg
     1081 tgggtaggct ttttagacac cgacaacctg atgagtcaga tttcgcagcg taaagagatg
     1141 aacgggttgc tggaacagaa aaagttctat acagaagaaa ttcagcggat gaaagaactt
     1201 tcgcagtcgc tgaaaaccga caaggaagca atggaacgtt atggtcgtga aaattatcag
     1261 atgaaaaaaa agtcagaaaa tatttttctg attgtccctc ccaaatctga cacgaaagaa
     1321 aaatagtctt tcagccctta tctccaaaat tcattatttt tttagtttgg ccatagtgta
     1381 atgctagttt tgcgctgtgg tataagttgt attgcctgcg aataggtgta ctttagcccg
     1441 acaaagcacc atgccaccga ctgtagtatt tcgcgcatag cgcgctagtt tggaaattcc
     1501 gctgtaatga tttcggttaa taccattact taatctaaat gagcccaaag cggaattttg
     1561 atttagtatt gtttatgccg atagagtaac tgttatagtt caaagtgcaa atgcgctatt
     1621 ggacgattac agataccgta tcggtattat ttttatattt gtaagagttt ttggcaggta
     1681 atggtctcgg ttttttacgc ttgcttccgt tgtttttcta tgagaaaata caccaatgaa
     1741 atcaggcaat aaggtaattt taatactgat aatgattgta gcttgtgcgc tgcgtttcta
     1801 tgattttttt gatattccct atactcatga tgaattcagt gcgcttttcc gtacacggtt
     1861 taatactttc gccgacctga tagacaaagg tgttaaagta gatacgctgc ctgccggtat
     1921 acagatattt ttgttttatt ggataaaaat ttttggtgaa accgaatgga tagtaaaact
     1981 tccttttctg ctgtttggct tgggtgcagt ttactttgta tatgtcgtgg gcaaaaaatg
     2041 gtataacgaa accgttgctc tcgttgcggc cgcttttctt gccagcttgc aatacacggt
     2101 tttttacagc caggtggcgc ggccgtatat ttcggggctg tttttttcgc tgctgatggt
     2161 taatgccctg accaatttga taaaaacccc cgaaaaacgg ttttattcca atatgctgat
     2221 atttgttttt gctgctgcgg cctgtgcgta taatcatcat ttcagcctgg tgtttgccgc
     2281 tatcgccggt gttagcgggc tgttcatgat acggaaagat tttttgataa aataccttgc
     2341 cgcgtgtttc gtcagtgtgt tattgtacct gcctcatatc aaaattgttt tggctcagct
     2401 gcaaatgggc ggaaatgaaa attggctcgg ggcatttgac tggagttttt tatacaatta
     2461 tttcaggtac atttttcagt tttcgatcat aagttacatt atggtgggct ttctgattgc
     2521 ttttgaaata ctgggttaca aaaaaagcaa tgctaagccc gggccgtcgc tattgttttt
     2581 gagctggttt gtacttcctt tgttggttgg cgtgttgtat tcggtatgtg tcaataatgt
     2641 aatgcagtat tctgtgctga ttttcagttt cccgtatttg ctgtttctgt tgttcggaca
     2701 tattcctaag cagaaaacca taatcaatct gctgttggtt tttttgatac tttgcgtcaa
     2761 tgtaagtgcg ttggttttcg aacgccatca ctattcatcg ttgtacgagt cggcatacct
     2821 gcatatattg taagatgggg aggcggccgt gaaaaactac aagaatgtgg taacccttat
     2881 tgataccgac aaggaaatat cggactatta cctgcaaaag gaaacataca ctgctcacta
     2941 tacttttgtt gatactacct ttacccgtaa gcgttttgtc tctttcctca aagaaagcag
     3001 ccggcattca ccctattttt attatggatg cttg
//
LOCUS       DAOSFP010000292         3031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30618_length_3031_cov_3.194892, whole genome shotgun sequence.
ACCESSION   DAOSFP010000292 DAOSFP010000000
VERSION     DAOSFP010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30618_length_3031_cov_3.194892"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2356
                     /locus_tag="PLI16_08375"
     CDS             <1..2356
                     /locus_tag="PLI16_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84613.1"
                     /translation="SVPANQEISIVFSFIGYQKVTHTLKLAAGESKKMNQIMVSSTTQ
                     IPIFEFFGQKDERALITRIDHKQAEFIPTTSGNAIEELIKKTSLGVYSSNELSSAYSV
                     RGGNFDENLVYVNDVEVYRPLLLRSGQQEGLSFVNSDLTSSVLFSSGGFDTKYGDKMS
                     SVLDIQYKKPKKTAGSIMLSLLGASGHIEAASKNERFTFLLGVRQKSNQYVLRAMETK
                     GQYKPSFTDVQLYTTYQISKKIEISLLGNYARNKYMVVPESRETEFGTVKESYQLKIF
                     FDGKELDYINTYLGALSLAYKPRSDLFFKWNNSVYQTRESQSYDVQGQYLIGRLEADF
                     GEEDFGEMKEVLGVGTHFQHARNELIATVINSDIKGGYTKKNHYLQFGVKYQHEIIDD
                     KLKEWEMIDSSGFTLPHMPDSVGYTNPWLQPANPLLLQEVVNTKVAMQSNRFSGFIQD
                     TWTFNIDSAELLLTYGVRAVYWDFNKQICISPRINLSFKPNWKTDIVFRFASGIYNQP
                     PFYKEIRAFDGSIHPEVKAQRSIHFLLGGDLNFKLWNRPFKFVGEVYYKMLDKLIPYE
                     IDNVRLRYFANNSGKGYAYGLDLKINGEFVKGDESWLGISLMRTREKIIGETFTDYYN
                     ASGEKIIFGYTADNVVVDSVVRQPGYIPRPTDQLLNINIFFQDHFPRWPTFKMHLNLV
                     YSSPLPFGPPEHKRYLDTLRMPAYFRVDIGFSKSLIDESTIFRKKNPLKIFKSLWISG
                     EVFNLLGRMNTVSYLWVKDVNNRQYAVPNELTKRLFNIKLVAKF"
     gene            complement(2426..>3031)
                     /locus_tag="PLI16_08380"
     CDS             complement(2426..>3031)
                     /locus_tag="PLI16_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84614.1"
                     /translation="QVPEKIPEKVLRVAEMVKQKYDLRVSQFKSSKEILPYAGKMFKT
                     YNEAFRHLYGFTPLTDRQIKFYIDQYFSMIDPKYVKFIVDRNDEVVGFGFCFLSLSKA
                     LIKAKGRLFPFGFIPVLWSMKHNDTVDLIMQGVKDEYHAKGVPAIFYASIMQVCIDNG
                     ITKAIASSVLEQNKESMLLFTNGYEKRMHMRRRSYKKILSA"
BASE COUNT          896 a          677 c          650 g          808 t
ORIGIN      
        1 ctcggtgcct gccaaccagg aaatcagcat tgttttttca tttatcggct accaaaaagt
       61 aacccatact ctgaaacttg cggcaggaga atcaaaaaaa atgaaccaga tcatggtgtc
      121 aagcaccaca cagataccta tctttgaatt tttcgggcaa aaagatgaaa gagccctcat
      181 tacgcgcatc gaccacaaac aagccgaatt tattccgacc acttcaggaa acgccatcga
      241 agaactgata aagaaaacca gcctgggcgt atattccagc aatgagctca gctcagctta
      301 ttcggtgagg ggcggcaact tcgacgaaaa cctggtgtat gtcaacgatg tagaagtgta
      361 ccgtcccctt ttgttgcgtt cgggacagca ggaaggccta agctttgtca attccgacct
      421 gacttcctcg gttctttttt cgtcgggagg atttgacacc aaatacggcg acaagatgtc
      481 gtcggtgctc gacatccaat acaaaaaacc taaaaaaacc gccggctcca ttatgctgag
      541 cctcctggga gcatccgggc atattgaagc cgcctcaaaa aacgaacggt ttacgtttct
      601 gctcggggtt cggcaaaaat caaatcaata cgtgctgcgt gccatggaaa ccaaagggca
      661 gtacaaaccg tcgttcaccg atgtgcagct atacactaca taccaaatca gcaaaaaaat
      721 cgaaatcagt ctgctgggaa attacgcccg caacaaatat atggtagttc ccgaatcgcg
      781 tgaaaccgag tttggtaccg tcaaagaatc ttaccaactg aaaatatttt tcgatgggaa
      841 agaactggat tatataaata cgtatctggg agccctctcg ctggcttaca aacccaggtc
      901 agacctgttt ttcaagtgga ataattcggt gtaccagacc cgggagtctc aaagctacga
      961 tgtgcaggga caatacctga ttggcaggct ggaagcagat tttggcgaag aagatttcgg
     1021 ggaaatgaaa gaggtactgg gtgtgggcac tcattttcag catgcccgca acgagcttat
     1081 tgccaccgtg ataaactccg acatcaaggg cggatatacc aaaaaaaatc attacctgca
     1141 gtttggcgta aaatatcagc atgaaataat tgatgacaag ttaaaggaat gggaaatgat
     1201 tgactcctct gggtttacgc ttccacacat gcctgattcg gtgggctaca ccaatccttg
     1261 gctacagcct gccaacccgt tgctgttgca ggaagtggtc aatacaaagg tagccatgca
     1321 aagcaaccgt ttttcgggat ttattcagga cacctggact tttaatattg acagtgctga
     1381 actgttgcta acttatggcg tcagagctgt gtactgggat ttcaacaagc aaatctgcat
     1441 cagtcccaga atcaatttat cgttcaagcc aaactggaaa accgacattg tcttccgttt
     1501 tgccagcggt atctataacc agccaccttt ttataaagaa atcagggcat tcgacgggag
     1561 catacaccct gaagtgaagg cgcagcgctc catacatttc ctgttgggcg gcgacttgaa
     1621 tttcaaattg tggaacaggc cctttaaatt tgtcggggaa gtttattata agatgctgga
     1681 caaattgatt ccgtatgaga tagacaatgt gcggctgcgc tatttcgcga ataacagcgg
     1741 aaagggttat gcctatggcc tcgatcttaa gataaacggc gagtttgtga aaggcgatga
     1801 atcgtggctg ggtatttcgc tgatgcgcac ccgcgaaaag attattggag aaacattcac
     1861 cgattattac aatgccagcg gagaaaaaat tatttttggc tataccgccg ataatgtggt
     1921 ggtagatagt gtggtgcgcc agccgggtta tattcccagg cctacggatc aattgctgaa
     1981 tatcaacatc ttttttcagg accattttcc acgctggcct actttcaaaa tgcacctgaa
     2041 cctggtttat tccagtccct taccattcgg tccacccgaa cacaaaaggt acctggatac
     2101 cttgcggatg cctgcttatt tcagggtgga tataggtttt tccaaatccc tgattgatga
     2161 aagcaccatt ttccgcaaga aaaacccctt gaagatcttc aaatcgctgt ggatttcggg
     2221 tgaagtgttc aacctgcttg gacgcatgaa caccgtttcg tacttatggg taaaagatgt
     2281 taacaacagg cagtatgccg tgcccaacga acttaccaaa cgcttattca acatcaaatt
     2341 agtggcaaaa ttttaaaaac atcttttatt gtaacacatt tattgttaaa gtgttcatag
     2401 tgtttatgcc acaaaaacgg aataatcagg cggacaaaat tttcttataa gaccttcttc
     2461 gcatgtgcat acgtttctcg taaccattgg taaacaacag catcgactct ttattctgtt
     2521 ccagcactga gcttgctatg gctttggtaa ttccattatc aatacatacc tgcataatag
     2581 aagcataaaa aatggccggc acccctttgg cgtgatattc gtcttttacc ccttgcatta
     2641 tcagatcaac agtatcatta tgcttcattg accacagaac agggataaaa ccaaacggga
     2701 acaatctgcc ttttgctttt attagagctt ttgataacga taaaaagcaa aatccgaaac
     2761 ctacaacctc atcattacgg tcaacgataa atttgacgta tttcggatct atcatcgaaa
     2821 aatactgatc aatataaaat tttatctgcc tgtcggtaag gggtgtaaat ccgtaaagat
     2881 gtctgaaagc ctcattatag gttttaaaca tcttgccagc atagggcaaa atctcctttg
     2941 aggatttgaa ttgtgacaca cgcaggtcat atttttgctt gaccatttcg gcgaccctta
     3001 gcactttttc aggaatcttt tcaggaacct g
//
LOCUS       DAOSFP010000293         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30833_length_3016_cov_3.104694, whole genome shotgun sequence.
ACCESSION   DAOSFP010000293 DAOSFP010000000
VERSION     DAOSFP010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30833_length_3016_cov_3.104694"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..2265)
                     /locus_tag="PLI16_08385"
     CDS             complement(103..2265)
                     /locus_tag="PLI16_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOH84615.1"
                     /translation="MKKFFLIWVLISFHSTIFAQCNYVFTIKNTHGQTMPGIEVTAKN
                     IEKNIILKSKTNSLGTATFTLTEGGTYTFSYLEINEAATLNLPEGFKGKGGKTVTYDP
                     EKIFTEKTKSDRTGISFTSRYPQQLKGQPNVVKFMIDLRKNNETAIPNTAVEIVDCKD
                     KVKYKGTTDAGGIATFYLPVNKAYEVDVDGNQAVHTFTVPDKTNAEFKNVVYFERTRV
                     KETSKGDTIIQANITQTQGTSTHLLFTISLVGFDDQPLPNEKVYLDAENSRKVYAGTT
                     DKNGSCKFMLEKGTNYLVNLTYENGIHLVEAPSSLGFAAASITRRYRGTAAIKQMMAQ
                     RHVNEKGFVVNHEQTPVRAAKRPSNYLKITNQGFDIDFATSGPIGTSTIAGNKLFTQE
                     GFYSPNFYCLQALTGEYQWGIELGESGASPIVYHNGVLLINTYSCTLYAFDASTGKML
                     WSKWLAGTIYSTPSADSNSVYVVYNNGYANPSNPSESFVVTSFDLRSGKMNWINWIDK
                     EVIACPVVEGNEVHVASQSGNYYVFDKVSGKQNLFSNNIKAVSSPTLTPQYIYVTTSV
                     NGREQLAVLDRKTLQLQRKYPTPLATKKISNRYSCYEQMNYNGAHPVVYKNKIVIITD
                     STKVAAFDANSEKILWQQAVSTHPNQMPLVADEKLVLATTTGEIKTFDLATGTLLKTQ
                     QHKGAIDGQLLTNKGYMYVGSDGALSAVKESRDMQWNQ"
     gene            complement(2481..>3016)
                     /locus_tag="PLI16_08390"
     CDS             complement(2481..>3016)
                     /locus_tag="PLI16_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84616.1"
                     /translation="TSEASNLNAYWNPATDSNSGISQYFYALGTTPGACDVIPFTPLN
                     DTFAVCNNLNLQNFQMYYFSVKAKNMAGLESDFTTSDGIFTDILYTSAGTPADRPFQI
                     FPNPAGNYLNIYGTDPGTKLQFRLTDAIGRTVISMPLQSKNQHQINLSDISEGIYFYC
                     ITDGTQQMDKGNLLIIR"
BASE COUNT          698 a          627 c          735 g          956 t
ORIGIN      
        1 taacagtatg gaaaatatcc tgaagacggt tttttcttaa aaatctgcgg aacagggtga
       61 ttatttttat ttataataga tattgtggcg ggcgtcttta tttcattgat tccactgcat
      121 gtccctgctt tctttaacgg ccgacagtgc gccatcggat cccacgtaca tatacccttt
      181 gtttgtcaat agctgtccgt ctatagcgcc tttatgctgc tgggttttca gaagggtacc
      241 ggtagccagg tcgaaagtct ttatctctcc ggtggttgtt gccaacacca gtttttcgtc
      301 ggcaaccagg ggcatctggt tgggatgggt gctgacagcc tgttgccaga ggattttttc
      361 gctgttggca tcaaaagctg ccactttggt gctgtcggtg atgattacaa ttttgttttt
      421 atagaccacc ggatgggctc cgttatagtt catctgttca tagcaggagt agcggttgct
      481 gatttttttt gtggcaagcg gggtgggata ttttctctgt agctgtaagg tctttctgtc
      541 gagcacagcc agttgttccc tgccgttgac ggacgtcgtt acatagatgt actgaggcgt
      601 aagcgtggga gaggatacag ccttgatgtt attggagaac aggttttgtt tacccgacac
      661 tttgtcaaag acatagtagt tgccgctttg tgaggccaca tgcacctcat ttccttccac
      721 cacgggacaa gcgataactt ctttgtcgat ccagttgatc cagttcattt ttccgctgcg
      781 cagatcaaaa ctcgttacaa caaaactttc ggaaggattg gaaggattgg cgtatccgtt
      841 gttgtacact acatacacgc tgttgctatc tgccgaaggc gtggagtata tagtgcccgc
      901 cagccatttg ctccacagca ttttgcctgt ggaggcatca aaagcatata atgtgcagga
      961 ataggtgttg attaaaagca ctccgttgtg gtacactatc ggcgaggccc ccgattcgcc
     1021 cagttcgatg ccccactgat actctcctgt aagggcttgc aggcaataaa aattcgggga
     1081 gtaaaaacct tcctgagtga ataatttgtt accggcaatg gttgaagtgc ctatgggtcc
     1141 cgaggtggca aagtcaatgt cgaaaccctg gttagtgatc tttaaataat tggatggtct
     1201 tttggccgcc cgtaccgggg tttgctcatg attaaccaca aaacctttct cgttgacatg
     1261 cctctgagcc atcatttgtt ttatggcagc cgttcctctg tagcgccggg tgatggaggc
     1321 cgcagcaaaa cccaggctgg atggcgcctc aaccaggtgg atgccgtttt cataggtgag
     1381 gtttaccagg taattagtgc ctttttcaag cataaacttg caggaaccat ttttgtccgt
     1441 agtgccggcg taaacctttc tgctgttttc cgcatcaaga taaacctttt cattgggcag
     1501 tggctggtca tcaaagccca ccaggctgat ggtaaacagc agatgcgtgg atgttccctg
     1561 ggtctgtgtg atgtttgcct gaattatcgt gtcgcctttt gaggtttctt ttacgcgggt
     1621 tctttcgaag tacaccacat ttttgaattc ggcattcgtt ttatcgggca cagtaaatgt
     1681 atggaccgcc tgattgccgt cgacgtcaac ctcgtatgct ttatttacag gtaaataaaa
     1741 agtggctatg ccaccggcat cggtagttcc cttgtacttg accttgtcct tgcagtcaac
     1801 aatttccacc gctgtgtttg gtatggctgt ctcgttgttt ttccgcaggt cgatcataaa
     1861 tttcaccaca ttgggttggc cttttagttg ttgcggatac cggcttgtaa agctgatgcc
     1921 ggtccggtca gattttgttt tttctgtaaa tattttttcc gggtcgtagg tcactgtttt
     1981 gccgcctttt cctttaaacc cttcgggaag gttcaaggtg gctgcttcat ttatttcaag
     2041 atagctgaaa gtgtacgttc ctccttcagt aagggtgaat gtggcggtcc ccaggctatt
     2101 tgtttttgat tttaagatta tgtttttttc aatgtttttt gcagttacct ctatgccagg
     2161 catagtctga ccgtgggtgt tcttaatggt aaaaacatag ttgcactggg cgaaaattgt
     2221 gctgtggaag ctgattaaca cccatatcaa aaaaaacttt ttcataagct tgaatttaat
     2281 aaatggatag ggataacagg cggcaaatta ctgtaataac gaaaaccact cccgggatat
     2341 ttttggagaa aaaaataaat ataactcttc agaaaattct gaagatcatc cgcacttttt
     2401 tttgttgctg tgcgcttctg ataccggcaa atttatatgt caggttctct ttttttctta
     2461 tttattacga atcaataggt tcagcgaata atcaggaggt tgcctttgtc catttgttgt
     2521 gttccatccg tgatacaata gaaataaatg ccttcggaga tatcgctcag gttaatctga
     2581 tgttgatttt tactttgcaa aggcattgaa ataaccgtgc ggcctatcgc gtcggtcaac
     2641 cggaattgca gtttcgtgcc gggatccgta ccataaatat tcaggtagtt tccggccgga
     2701 ttggggaaga tctggaatgg cctgtcggca ggggtaccgg ctgaagtata aagaatgtcg
     2761 gtaaatatcc cgtcggaggt agtaaagtca gattccagtc ctgccatgtt tttggctttt
     2821 actgaaaaat aatacatctg aaagttttgc aggttcaggt tattacatac cgcaaaagta
     2881 tcgttcaacg gggtgaaagg tattacatcg caggctcccg gagtggttcc taatgcatag
     2941 aagtactgcg atatgccgct gtttgaatct gttgcagggt tccagtatgc attcaagttc
     3001 gatgcttcgg aggtgg
//
LOCUS       DAOSFP010000294         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30849_length_3015_cov_3.700000, whole genome shotgun sequence.
ACCESSION   DAOSFP010000294 DAOSFP010000000
VERSION     DAOSFP010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30849_length_3015_cov_3.700000"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..1969)
                     /locus_tag="PLI16_08395"
     CDS             complement(11..1969)
                     /locus_tag="PLI16_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018463067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HOH84617.1"
                     /translation="MQDNKSVKSKVSFFSVIMTLGIVYGDIGTSPLYVMRAVLGSSEA
                     ITPGFVIGAISCVIWTLTLLTTLKYVVIALRADNKGEGGIFSLFALIRKKAPWIFVFA
                     IIGGATELADGVITPSITVVSATEGLLLVNSHVPVIPIALLIILALFSYQKFGTKVVG
                     SSFGPIMLMWFTMLGIVGTMYVVRAPYIFKAFNPYYAIDFLIGHPGGFVLLGAIFLAT
                     TGAEALYSDLGHCGKNNIRISWMFVKLTLILNYLGQGSWILLNMDQVTHTTNPFFGMM
                     QDVSFLIPAIILATLAAIIASQALICGAYTLISVAMSLNLWPRLRISYPTNIKGQMYI
                     SSVNWALCIACFFVVLFFKESNNMTAAYGLSITITMLMTTILLSYYLLRIVKVHILVV
                     ALFMMTFLTIEGSFLIANLHKFMSGGWFTIMIGGFLFSIMFIWQKGRSLKNRFIEFTR
                     IAPFAELLKDVKKDTTIPKYATNLVYITKANHVGDVETKIIYSIINKQPKRADLYWLL
                     HINITDDPNTKEFKVTTIIPDTLIKIDFNLGFRVQPRINLFFKQVVDEMIRNNEIDMI
                     SRYPSLQKHHVLADFRFILIDRIQNYDFDFPPFEQFVMDIYTYLKKIGITEVRAFGLD
                     TSNVVIETVPLSSMKNPKFDLKRIRDLS"
     gene            complement(2095..>3015)
                     /locus_tag="PLI16_08400"
     CDS             complement(2095..>3015)
                     /locus_tag="PLI16_08400"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOH84618.1"
                     /translation="WKNKLSQAACIPAPDFMLLHNEYGRYCGHQVKKFLSKNHFTPDF
                     ISSHGHTIFHQPEKQLTVQIGSGAALAAETGITTVSDFRITDVALHGQGAPLVPIGDA
                     LLFADFDICLNIGGISNLSYQKKSQRIAYDVSPANMVLNYLARKAGKDYDDGGKMAST
                     GKVSLPLLTMLDQLDYYKIEPPKSLGREWIETAFLALLDSMPEISLEDKLRTCTEHIA
                     GQIARQINAIKAGKVLVTGGGALNKFLMSRIKQQCKVDIVIPGKETVEYKEALIFAFL
                     GVLRMRNENNCLKSVTGAKKDNCGGTVFLP"
BASE COUNT          869 a          638 c          678 g          830 t
ORIGIN      
        1 attgctatta tcaacttaaa tcacgaatgc gtttcaggtc aaatttaggg tttttcattg
       61 acgaaagagg caccgtctct atgacgacat tgctggtgtc aagtccaaaa gcccgaactt
      121 cggtaatgcc gatttttttc aggtaagtat agatatccat tacaaactgt tcgaacgggg
      181 ggaagtcaaa gtcataattc tgaatgcgat cgatgagaat aaaacggaaa tcggccagca
      241 catggtgttt ttgtagggag ggatacctcg atatcatatc aatttcgtta ttacgaatca
      301 tttcatcgac cacttgcttg aagaacaggt ttatcctcgg ttgtactctg aaaccgaggt
      361 tgaaatcaat ttttataagg gtgtcgggga ttattgtggt aactttaaat tctttggtat
      421 tagggtcgtc ggtaatgttg atgtgtaaca gccagtaaag gtcggctctt ttaggctgtt
      481 tgttgatgat agaataaata atttttgttt ccacatcgcc gacatggttg gccttggtga
      541 tatataccag gttagtggca tattttggaa tggtagtgtc tttttttaca tccttcagga
      601 gctctgcaaa aggggctatt cgtgtaaact caataaatct gttttttaac gaccgtcctt
      661 tttgccagat aaacattatg ctgaataaaa atccgccaat cattatcgta aaccatccgc
      721 cgctcataaa tttgtggagg ttggctatca gaaaactacc ctcgatggtg agaaatgtca
      781 tcataaataa agcaaccacc aggatatgaa cctttacaat ccttaaaaga taatagctga
      841 gcaaaatagt ggtcatcagc atggtaatgg taatagacag accataggcg gcagtcatgt
      901 tattggattc cttgaaaaat agcacgacaa aaaaacaggc aatacacaag gcccagttta
      961 ccgaactgat atacatctgc cctttgatgt tggtgggata agatatcctg aggcggggcc
     1021 acaaattaag cgacatggcg accgaaatga gggtataggc accgcatatc agggcctggc
     1081 tggcgataat ggctgccagg gtagccagga taatggccgg aataagaaat gaaacatcct
     1141 gcatcatgcc aaaaaatggg ttcgtggtat gagtaacctg atccatatta agtagaatcc
     1201 atgagccttg ccctaaataa ttcagaatga gcgtcaactt cacaaacatc cagctgattc
     1261 ttatgttgtt ttttccgcaa tgtcccaaat cggaatacaa ggcctcggct cctgtggtag
     1321 ccagaaaaat agccccgagg aggacaaagc cacccggatg ccctatcaga aaatcaatgg
     1381 cataataagg attaaacgcc ttgaaaatat atggagcacg aacaacatac atcgtgccga
     1441 cgatgcccag catggtaaac cacatcagca tgataggccc gaaggaactg cccactactt
     1501 tggtgccaaa tttctgatag ctgaaaagtg ccaggataat cagcagggct attggaataa
     1561 caggaacatg ggaatttacc aataataaac cttcggtcgc ggaaaccacc gtgatggagg
     1621 gagtgataac accatcggcc agttcggtgg caccgccaat aatggcaaaa acaaagatcc
     1681 agggtgcttt tttcctgata agtgcaaaca acgaaaaaat accgccttca cccttgttgt
     1741 cagcccgcag ggcaataacc acatatttca acgttgtaag taaggtgagc gtccatatga
     1801 cacaggatat cgcaccgata acgaacccgg gagtgattgc ctcgctgctt ccaagtacag
     1861 cacgcatcac atataacggg gacgtgccga tgtctccgta tactattccc aatgtcatga
     1921 tgacactgaa aaaagaaact ttggatttga cagatttgtt atcctgcatt tttaataata
     1981 aaacggcaaa tataaatcaa caatgacctc aaaatctatt tattcaaaaa tatttttttc
     2041 aacagccggc caccaggtaa tggccatggt tttgttgtgt ttttgatgag aacttcaagg
     2101 caaaaagaca gtcccgccgc agttgtcttt tttggcgccc gtaacgcttt tcagacaatt
     2161 gttttcatta cgcatacgta acactccaag gaaggcaaaa atcaatgctt ctttgtactc
     2221 cactgtttct ttaccaggga taacaatatc gactttacat tgctgcttta tcctgctcat
     2281 taaaaattta tttaacgcac caccgcctgt taccagtact ttaccggctt ttatggcgtt
     2341 tatctggcgc gcaatttggc cggcaatatg ttcggtacag gtacgtaatt tatcctccag
     2401 ggaaatttcc ggcatgctgt ccaaaagagc gaggaaagca gtttctatcc attctctacc
     2461 gagagacttg ggcggttcga tcttgtaata gtccagctga tccagcatgg tgagcagggg
     2521 gagggatact ttgcccgtag aagccatttt tccgccgtcg tcataatctt ttccggcttt
     2581 gcgtgccagg taattcaata ccatatttgc cgggctgaca tcgtaagcta ttcgttgtga
     2641 tttcttttga tacgatagat ttgaaatgcc tccgatattc agacagatgt caaaatcggc
     2701 aaaaagtaat gcatcgccaa tgggcaccag gggagctcct tgtccgtgca gggcaacatc
     2761 agttattctg aagtcgctca cggtggttat tccggtttcg gcagccagcg ccgcgccgct
     2821 gccaatctgc acggttagtt gtttttccgg ctggtggaaa atagtatggc cgtggctgct
     2881 gataaaatca ggggtgaaat ggtttttcga aaggaacttt tttacctggt ggccgcagta
     2941 tcttccgtat tcattgtgga gtaacataaa atcgggcgcc gggatgcagg ctgcctgcga
     3001 taatttgttt ttcca
//
LOCUS       DAOSFP010000295         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30872_length_3014_cov_3.224062, whole genome shotgun sequence.
ACCESSION   DAOSFP010000295 DAOSFP010000000
VERSION     DAOSFP010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30872_length_3014_cov_3.224062"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLI16_08405"
     CDS             <1..646
                     /locus_tag="PLI16_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOH84619.1"
                     /translation="VQKKSGNAQQVAFTAISILALAGVIVLFILFFRNKENTAANVVA
                     PSIDKVTFAYVNTDTIMQDYDFVLDVQVELANFEKSLQNQYASRAKAFEKEYNDYIKR
                     ATAGLLTLDQQKKKEEELSKEQMAIQELESNLALQLQEEKLKRNMEVHDTIVNTIARY
                     NKTKNFTFIFEKSYGGGLLFVHPAFDITEDILTVLNKEYEKKPTAGGSETEKAK"
     gene            657..1085
                     /locus_tag="PLI16_08410"
     CDS             657..1085
                     /locus_tag="PLI16_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84620.1"
                     /translation="MKIKILLLLLLAFFCAGCTTKIVEDTIESYPDGSPKMVRYYKDD
                     GDQRVLFKETLYYSNHQKYMEGEYKNGKRHGKWTSWYQNGNTWSEGSFNNGVDDGVRT
                     AYHENGKKHFEGKYKDGKKTGIWKFWDENGVQQKDQDYGQ"
     gene            complement(1086..2183)
                     /locus_tag="PLI16_08415"
     CDS             complement(1086..2183)
                     /locus_tag="PLI16_08415"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOH84621.1"
                     /translation="MRKAGVILLVLLFPIVFAAAQQPVKPVRNLRLETLVHYGFVIPH
                     HKTMQHLTQSHFPCFEVNLSEQTHGKQIWEQLFKYPLKGLGLYYSPLGNTRELGHALA
                     VYPYINFCLTHGKKVNLYFRVAAGLGYLTKSFDAETNYKNIAIGSHFNAFIQLRYELR
                     WHITDRLGFSAGISISHFSNACYKVPNFGINIPTLNMGLSYRLNKTPAAIIKNEIPAA
                     DKKWQFSILGSFGLNELAGPGGRKYPYYCLSGFFMKPASLKRQFGIGLDVYYSEALGT
                     LIQRSDSVSNIKYQAIRPGLSFAYIINFARLSFVAQLGAYLYTKEKTDGYIWDRVALR
                     YTVKDHYLIQMGLKTHLSRAEVLELSVGYKF"
     gene            2254..>3014
                     /gene="mazG"
                     /locus_tag="PLI16_08420"
     CDS             2254..>3014
                     /gene="mazG"
                     /locus_tag="PLI16_08420"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOH84622.1"
                     /translation="MRDELKAFERLLDIMDELRAKCPWDKSQTIESLRYLTIEETYEL
                     SESIIRNDMDDVRKELGDLMLHIVFYSKIASEKNAFNITDVLNGICEKLIVRHPHIYG
                     DVKVSNADDVKSNWEKIKLKTGEKSVLAGVPASLPAMVKAYRIQEKVSGVGFDWEKRS
                     QVFDKVMEEANELKNEVENNLSIEKTEQEFGDLIFALINYARYINVNPEDALEKTNIK
                     FIKRFSFIEKKAKESGRRIQELSLAEMDSYWNESKL"
BASE COUNT          990 a          620 c          647 g          757 t
ORIGIN      
        1 tgtgcaaaaa aaatcaggca acgcacaaca ggtcgctttt acggctataa gtattctggc
       61 gctggcaggc gtcatcgttt tgtttatttt atttttccgc aacaaagaaa atacggctgc
      121 caatgttgtt gctccgtcga ttgacaaagt aacatttgca tacgtaaaca ccgacaccat
      181 aatgcaggat tatgattttg tacttgatgt tcaggtggag ctggccaatt ttgaaaaaag
      241 cctccagaat caatatgctt ctagggccaa ggcctttgaa aaagagtata atgattatat
      301 caaaagagct accgcaggcc tgttgacact cgaccagcaa aagaaaaaag aagaagaatt
      361 gtccaaagaa caaatggcca tacaggaact ggaatcaaac ctggctttgc agcttcagga
      421 ggaaaaacta aagcgcaata tggaggttca cgataccatt gtaaacacca ttgcccgtta
      481 caacaaaaca aaaaatttca cttttatctt tgaaaaatct tatggcggcg gattgctgtt
      541 tgttcacccg gcctttgata tcaccgaaga tattctgacc gtgctgaata aagaatatga
      601 aaaaaaacca acagccggag gttcggaaac cgaaaaggcc aagtaatcgc taaactatga
      661 aaataaagat actgctgcta ttgctgcttg cttttttttg cgcaggctgt acaactaaaa
      721 ttgtggagga taccatagaa tcatatcccg atggctcccc caaaatggtt cgttattata
      781 aggatgatgg tgatcaacgg gtattgttta aggaaacttt gtattattcc aatcatcaga
      841 aatatatgga aggagaatac aaaaacggca aacgccacgg taaatggacc agctggtatc
      901 aaaacggcaa cacctggagc gaaggttctt ttaacaacgg agttgatgac ggtgtgcgta
      961 ccgcctacca cgaaaacgga aaaaaacatt tcgaaggaaa atacaaagac ggcaaaaaaa
     1021 ccggcatctg gaaattctgg gacgaaaacg gcgtgcagca aaaagaccag gactacggac
     1081 aataatcaga atttataacc cacactcaat tccagcacct cggctctgct aagatgtgtt
     1141 ttaagtccca tttgaattaa ataatggtct tttaccgtat aacgtaaagc cacccggtcc
     1201 cagatatagc catcggtttt ttctttggtg tataggtagg cgcctaattg ggcaacaaac
     1261 gagagccgtg caaaatttat gatataggca aaactcagcc cgggtctgat agcctgatat
     1321 ttgatgttcg acacgctatc gctgcgctgg ataagagttc ccagagcttc gctgtaatat
     1381 acatccaggc cgatgccaaa ttgccgcttc aggctggcag gcttcataaa aaaaccgctt
     1441 aaacagtaat aagggtattt tctaccgccc ggtcctgcca actcgttcaa tccaaaactg
     1501 cccaaaatgg aaaactgcca ttttttgtcg gcggcgggta tttcattttt tattatcgct
     1561 gccggtgttt tgtttaagcg gtacgacaaa cccatgttca gcgtgggaat attgatgcca
     1621 aaatttggca ccttataaca tgcattggaa aaatgcgaaa tacttatgcc tgccgaaaag
     1681 cccagccggt cagtgatatg ccacctcaac tcataacgga gttgtataaa ggcgttgaaa
     1741 tgcgacccaa tggcaatatt cttatagttg gtttccgcat caaaactttt tgtcagatac
     1801 cccaggcctg cagctacacg aaaatacagg ttgacttttt tgccatgggt aaggcaaaag
     1861 ttgatgtacg gataaacagc cagggcatgt cccagctctc tggtattgcc aaggggtgaa
     1921 tagtacaaac caaggccttt taatgggtat ttaaaaagtt gctcccatat ttgtttcccg
     1981 tgcgtctgtt ccgacagatt gacctcaaaa caaggaaaat gactttgcgt aaggtgttgc
     2041 atggttttgt gatgaggaat cacaaaacca taatgaacca aagtttccag gcgcaggttc
     2101 ctgaccggtt ttaccggctg ttgtgcagcc gcaaaaacaa taggaaataa taaaactaat
     2161 agaatgacgc ccgcttttct caaaataatt tttgttacat gccgtacaaa gataaaaaat
     2221 agaattgatt acatttgccg aaaacaaaac atcatgagag atgaattaaa ggcgtttgaa
     2281 cgcttgctgg atatcatgga cgaactgcgg gctaaatgtc cctgggacaa atcccaaacc
     2341 atcgaatccc tgagatatct taccattgag gaaacttacg aactttccga atccatcatt
     2401 agaaacgata tggatgatgt gcgcaaagaa cttggcgacc tgatgctgca tatagttttt
     2461 tattccaaaa ttgcttccga aaaaaatgcc ttcaatataa cagatgtgct gaacggcatc
     2521 tgcgaaaaac tgatagtgcg tcatccgcat atttatggtg atgttaaagt gtccaatgcc
     2581 gatgatgtaa aaagcaactg ggaaaaaata aaactgaaaa caggcgaaaa atctgtactt
     2641 gcaggcgttc ctgcttcgct gcctgccatg gtaaaggcat accgcataca ggaaaaagtc
     2701 agtggcgtag gcttcgactg ggaaaaacgc agtcaggtat tcgacaaagt tatggaagaa
     2761 gccaacgaac ttaaaaatga agtggaaaac aacctcagta ttgaaaaaac agaacaggag
     2821 ttcggcgacc tgatttttgc cctgatcaac tatgcccgtt acattaatgt gaacccggaa
     2881 gacgcccttg aaaaaaccaa tataaaattt attaaacggt tttcgtttat cgaaaaaaaa
     2941 gccaaagaat caggacgccg gattcaggag cttagtctgg ccgaaatgga tagctactgg
     3001 aacgaatcaa aact
//
LOCUS       DAOSFP010000296         3005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_30989_length_3005_cov_3.881017, whole genome shotgun sequence.
ACCESSION   DAOSFP010000296 DAOSFP010000000
VERSION     DAOSFP010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30989_length_3005_cov_3.881017"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLI16_08425"
     CDS             <1..634
                     /locus_tag="PLI16_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOH84623.1"
                     /translation="WLDQNETLLEKPDEIHCITTKELSAISLLTTPNQVLATIKIPSP
                     VFQAVMAAQSLIITLDDIRDPGNLGTIIRIADWFGFKNILCSETSVDAFNPKCVQSTM
                     GSIARVNIYYENLMDVFKNCPDNTNIYGAFLHGENIYDINPEPHGFLIIGNEANGIQP
                     EVAKLVTRHISIPAGSHAGLHAESLNASAATAILCSEFFRKTNHLTQTST"
     gene            631..1755
                     /gene="lpxB"
                     /locus_tag="PLI16_08430"
     CDS             631..1755
                     /gene="lpxB"
                     /locus_tag="PLI16_08430"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOH84624.1"
                     /translation="MKYYIIAGEASGDLHASNLMAAIKKNDPAAGFRCWGGDLMKAQG
                     GEIVKHIREISFMGFAEVLSHLRTILRNIRFCKSDIDRYNPDALILVDYPGFNLKIAA
                     YARQKGYKVIYYVSPQIWAWKQSRVHAIKKNVDKMLVILPFEKEFYKRFNYEADFVGH
                     PLLDAIDNYKNNSNSAQFRKENNLGVQPVVALLPGSRKQEITRMLKMMVSVCDDFKEV
                     QFVIAGAPSINPGFYSELLHGKEVKIVYNKTYDLLQNAAAALVTSGTATLETALFDVP
                     EVVCYKGSWFSYFIARLVIKVKYISLVNLIMDKKIVTELIQSELTKANLIAQLRQLLY
                     STQHITQLKSDYALLKHKLGDRGASAKAAAVINTYLKSGQ"
     gene            complement(1749..>3005)
                     /locus_tag="PLI16_08435"
     CDS             complement(1749..>3005)
                     /locus_tag="PLI16_08435"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HOH84625.1"
                     /translation="PLTPKQIERLIKHHKLDFVVQTSDKRVFTDDEYQKAGAKIVKDL
                     KKCDIVLGIKEMPEDIFENEKTYVFFSHVIKGQTYNMPMLRKMMDMKCNLIDYEKIVD
                     EQGKRLIFFGKFAGMAGMINTLWSFGLRLKELGIDNKLTRVKQSHKYHSLNEARNTIS
                     AIGQQISEDGLVEGLKPFVVGFTGYGNVSNGAQEILGLLPVKEISPEKLLTLKNRAHL
                     PNNIIYKVVFKEEDIYEHKDGKEFDLHDLYANPEDYKSKFEQYVPLLTILMNCMYWDA
                     RYPRILTKEYLKKLFQKGQPKLTAIGDITCDPDGSIECTHDGTHIEDPIFVYNPLTDT
                     YAMGYKGEGILDMAVSILPSELPRDSSESFGDMLINFIKPIANADFDLAFEDLDLPRA
                     LKKAMILHKGEFTPEYKYLEEYVKGY"
BASE COUNT          870 a          677 c          609 g          849 t
ORIGIN      
        1 gtggctcgac caaaacgaaa ccctgcttga aaaacccgat gaaatccatt gtattaccac
       61 aaaagaatta tcggccatca gcttgctgac aacccccaac caggtgttag ccaccatcaa
      121 aataccttcg ccggttttcc aggccgtcat ggcagcccaa tcgcttatca tcaccttgga
      181 cgatatccgc gaccccggca acctgggcac cataatccgt attgccgatt ggttcggatt
      241 taaaaacatc ctttgttccg aaacatcggt ggacgccttt aaccccaaat gtgtacaatc
      301 cacaatggga tcaattgccc gggtaaatat ttattatgaa aacctgatgg atgtttttaa
      361 aaattgtcct gacaacacta atatttacgg ggcatttctg catggcgaaa acatctatga
      421 cattaacccc gaaccccatg gatttctcat tatcggtaac gaagccaacg gcattcagcc
      481 tgaggtagca aaactggtaa ccagacatat aagtattccg gcaggcagcc acgctggttt
      541 gcatgccgaa tcgctgaacg cttctgcagc tacggccatt ctctgttctg aatttttcag
      601 gaaaaccaat cacttaacgc aaacttccac atgaaatatt atatcatagc cggagaagcc
      661 tcaggcgatc tgcacgcctc caacctgatg gcggctatca aaaaaaacga tcctgcagcc
      721 ggttttcgtt gttggggcgg cgaccttatg aaagctcagg gtggagaaat agttaaacat
      781 atccgcgaga tttctttcat gggatttgcc gaggtattgt cccatttaag aactatcctc
      841 cgcaatatcc gcttttgcaa atcagatatt gaccgttata atcccgatgc gttaatcctt
      901 gtagattatc ccggttttaa cctgaaaatt gccgcttacg cgcgtcagaa agggtacaaa
      961 gtcatctatt atgtctcgcc acagatttgg gcatggaaac aatcccgtgt tcatgccatc
     1021 aaaaaaaacg tggataaaat gttggtcata ctcccatttg aaaaggagtt ttataagcgt
     1081 tttaattacg aagcggattt tgttggacat cccttgctgg atgccattga taactataaa
     1141 aataacagca acagcgctca attcaggaaa gaaaacaacc ttggcgtaca gcccgtggtg
     1201 gcgctgctcc ccggaagcag gaaacaggaa ataacgcgta tgctgaaaat gatggtttcg
     1261 gtatgcgatg attttaagga ggtgcaattt gtcattgccg gcgccccgtc aataaacccc
     1321 ggattttatt cggagttgtt gcatggcaaa gaggtaaaaa ttgtttataa caaaacctat
     1381 gatttattgc aaaacgcagc cgccgccctg gtaacttccg gcacggccac actggaaacc
     1441 gcactgtttg atgtccccga ggtggtatgc tacaaaggat cctggttttc gtatttcatt
     1501 gcacggcttg taatcaaggt aaaatacatt tcgctggtca acctgattat ggacaaaaaa
     1561 attgtaacgg aattaattca atcagaatta acaaaagcaa atctaatagc acagctgcga
     1621 cagttgctat acagcacaca acatatcact caactaaaga gtgactatgc cctgttaaag
     1681 cacaaactgg gtgatagagg agcctccgcg aaggcggccg ctgttataaa tacttacctg
     1741 aagtcaggtc aataaccttt gacgtattct tcaaggtatt tgtactcggg tgtgaattct
     1801 cccttatgca gtatcatcgc ttttttcaag gcccggggca ggtcgaggtc ttcgaacgcc
     1861 aaatcaaaat cggcattagc aataggctta ataaaattta tcagcatatc gccaaaggat
     1921 tcggaagagt cgcgcggcaa ttcgctgggc aatatggata ctgccatatc cagtatgcct
     1981 tcgcctttat aacccatggc ataggtgtcg gtcagcggat tataaacaaa aatgggatct
     2041 tcaatgtggg ttccgtcgtg ggtacattct attgaaccat caggatcaca ggtgatatca
     2101 cctatggctg taagtttggg ttgccccttt tgaaacagtt ttttcaggta ttctttggtg
     2161 agaatccggg ggtaacgtgc atcccaatac atacaattca tcagtatggt caggagcggg
     2221 acatattgtt caaatttact tttataatct tcggggtttg catacaaatc atgcaaatca
     2281 aattctttac catccttatg ttcgtaaatg tcctcttctt taaaaaccac cttataaata
     2341 atattattgg gcaaatgggc tcggtttttt aaagtaagta atttttcggg cgagatttct
     2401 ttaacaggca aaagtcccag aatttcctga gcgccattag aaacattacc atagccggta
     2461 aatcccacca cgaatggttt taatccttcg acaagcccgt cctccgaaat ttgctgtccg
     2521 atagccgaga tggtattgcg ggcttcattc agcgaatggt atttatgcga ctgcttaacc
     2581 ctggtaagtt tgttgtcgat tcccaattct tttaaccgaa gaccgaagga ccacaaggta
     2641 ttgatcatac cggccatgcc agcaaatttt ccgaaaaaaa tcaaacgttt accctgttca
     2701 tccacaattt tttcataatc aataaggttg catttcatat ccatcatctt acgaagcatg
     2761 ggcatattat aggtctggcc ttttatcaca tgagaaaaaa acacataagt tttttcattt
     2821 tcaaaaatat cttccggcat ttcttttatg ccaagaacaa tatcacactt ttttaggtct
     2881 ttgactatct tagcaccagc cttttgatat tcatcgtcag taaataccct tttgtcggat
     2941 gtttgaacca caaaatccag cttatggtgc ttgatcaatc tttcaatttg tttaggtgtt
     3001 agagg
//
LOCUS       DAOSFP010000297         2995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_31141_length_2995_cov_4.530272, whole genome shotgun sequence.
ACCESSION   DAOSFP010000297 DAOSFP010000000
VERSION     DAOSFP010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31141_length_2995_cov_4.530272"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..626)
                     /locus_tag="PLI16_08440"
     CDS             complement(90..626)
                     /locus_tag="PLI16_08440"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOH84626.1"
                     /translation="MKDSTRHIAGYFVGGSVFLLLIPYCFYMISVREYPVFHDELAIS
                     ATVRIIISVPVILTGLVFAAWSNIFLFSVGKGGPVDVFNKAISPRTKKLVISGPYRYS
                     RNPMVFGAFCLYTGIAIGLNSARCLIISIIFLLIMVVYIKIFEEKRLLKDFGDEYLAY
                     KKNVSMIFPLKFRKKKRG"
     gene            complement(752..1360)
                     /locus_tag="PLI16_08445"
     CDS             complement(752..1360)
                     /locus_tag="PLI16_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOH84627.1"
                     /translation="MKKIVFGLFLLTCTCMLNSCEKKVGGCTDQYAINYNADANENDG
                     SCISIGSPYEGGILAYILQPDDPGYDANVKHGLVVDTTYQFSTIIWGCPGVATGGTST
                     ALGSGQANTTAIVNGCSESGAAYVCDTLVSGVYDDWYLPSRDELEKLYKSKDIIGGFA
                     SDRYWSSSESNEHDAWSHDFGNDSIKENHKYHPYYVRAVRSF"
     gene            complement(1446..2426)
                     /locus_tag="PLI16_08450"
     CDS             complement(1446..2426)
                     /locus_tag="PLI16_08450"
                     /inference="COORDINATES: protein motif:HMM:NF021829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOH84628.1"
                     /translation="MVLCLCFIISVLCVQGQTVNDTAIRYVHTQTPGLMSCIIKTAAR
                     LFLPKNSIPRKLSNDNFTSAPAAIPKTVATDLFIDTLRVHGRNVFTLAPKGTKSAKVV
                     LFLHGGAYINNIFRQHWSFAAKIIRETNCTFIIPDYPLAPAYTFQEAFDMLETLYKQL
                     LGTANEKDIVLMGDSAGGGLALALAMKLRDGGTALPAAVILLSPWLDISMTNPQIKEV
                     QKKDVSLRAENLVLAAKAWAGTTETNHYLLSFVYGSLGGLPKISIFIGTHDILEPDCR
                     KLKSLMEQKNIAMNYFEYPKMFHDWMMLGPLRESKLARAQICSLILDKKQ"
BASE COUNT          871 a          653 c          613 g          858 t
ORIGIN      
        1 gttaaaatag aggccacgga ctttagtcct gacagccggc ggaaagattc aagcttattg
       61 ccggtaaatt aaaaatcaaa ataaaagttt taccctcttt ttttcttcct gaattttaac
      121 gggaatatca ttgacacatt tttcttatac gccagatatt catcaccaaa atccttcagt
      181 aatcttttct cttcgaatat tttaatataa acgaccatga tgagcaaaaa gataatggaa
      241 attatcaggc acctggcaga attcagccct atggcaatac cggtatataa acaaaaagcc
      301 ccgaagacca tcgggtttct gctgtatctg tatggcccgc tgatcacaag tttttttgtc
      361 ctgggactga tggctttgtt aaaaacatcg accgggccac ccttcccgac actgaacaaa
      421 aatatatttg accaggcagc aaataccaat cctgtgagaa tgacaggtac tgaaattatt
      481 atcctgacag ttgccgaaat ggccagttca tcatggaaca caggatattc cctgaccgat
      541 atcatgtaaa aacaatatgg tatcagcaaa agaaaaaccg atccgccaac gaaatatcct
      601 gcaatatgtc tggtagagtc tttcattatc gcgatatttt gttgtggatg tttattgtat
      661 tgcaggtgtt caaaaactta taccgtatta atgattgcta agtgtcagtg cgaaatctgc
      721 aacttcgcac atcctacaca ccctgtccca atcaaaacga ccttacagcc cttacataat
      781 acgggtggta cttgtgattt tctttgatac tgtcgttgcc gaaatcgtgc gaccaggcat
      841 cgtgttcatt gctttcggag ctgctccagt aacggtcgct ggcaaaaccg ccgataatat
      901 ctttagattt gtaaagtttt tccaactcat cgcggctcgg caggtaccag tcatcataaa
      961 cgcccgagac cagggtgtcg catacatagg ccgcgcctga ttcactgcag ccgtttacaa
     1021 tggctgtggt attggcctgt ccgctgccaa gcgccgtgga cgttccccct gtggccaccc
     1081 ccggacatcc ccagattatg gttgagaact ggtatgtggt gtccacaacc aaaccgtgtt
     1141 ttacgttggc atcataacca ggatcatcgg gctgtaagat ataggcaaga ataccgcctt
     1201 cgtaaggcga gccgatactg atacaggaac cgtcgttttc gttggcatcg gcattataat
     1261 taattgcata ctggtcggtg caaccgccaa ccttcttttc gcaactgttc agcatacaag
     1321 tacatgtcaa taaaaacaaa ccaaaaacta tttttttcat tttatttttt ttaagagtgc
     1381 aatttacaac tatttttcaa tctaattctc caaaacggac gttacatttt tttggcgatt
     1441 caaaattatt gttttttgtc cagtatcaac gaacaaattt gtgctcttgc cagcttcgat
     1501 tctcttaggg ggccaagcat catccagtcg tgaaacatct ttgggtattc aaagtaattc
     1561 atggctatgt tcttctgctc catcaacgac ttgagtttcc tgcaatccgg ctctaatatg
     1621 tcatgggtgc caataaaaat gctgattttg ggcagtcccc ccaatgagcc ataaacaaaa
     1681 ctaagcaggt aatgattcgt ttcagtggta cctgcccagg cctttgctgc cagcaccagg
     1741 ttttctgccc gcagcgaaac atcttttttt tgaacttctt taatttgcgg gtttgtcatg
     1801 ctgatgtcga gccaggggga taaaagaata actgccgcgg gcaacgccgt accaccatcc
     1861 cttagtttca tagccagggc gagggcaagc ccgccgcccg ccgagtcgcc catcaggacg
     1921 atatcttttt cgttggccgt gccgagcagt tgtttataaa gggtttcaag catgtcaaaa
     1981 gcctcctgaa aggtataagc gggcgctagc gggtaatcgg ggattatgaa tgtgcagtta
     2041 gtttcacgga taatttttgc ggcgaaactc cagtgctgtc ggaaaatatt attgatatag
     2101 gcgccaccat gaagaaacaa aacaacttta gcgcttttcg tccctttggg tgccagggta
     2161 aaaacattcc ggccatgtac ccgcaaagtg tcaataaaaa gatcggtcgc cactgttttg
     2221 ggaatggctg ccggcgcaga ggtaaaattg tcgttgctca gttttcgcgg gatgctgttt
     2281 ttgggcaaga atagccttgc cgcggtctta ataatacaag acataaggcc gggagtttgt
     2341 gtatgcacat acctgatagc ggtgtcatta accgtttgcc cttgcacaca caaaactgaa
     2401 ataataaaac acaaacagag aaccatcctt tttatccggc agcgcactat gcctttgcag
     2461 ttgagaaagt cagtcattgt ctttagaaat aagatggcat aaaaaaaact tcaggaacaa
     2521 aaataattta atttaataac aaattttact tgcttaaacg gcccaatcat tttttgatgt
     2581 gtttagagat ttttttattt taaataactc aactctcgga attcagataa aaaaaaaaca
     2641 gaaaatctct ttctgttatt aataagttgc tcattaatgc cattgctaag acctgtgtgt
     2701 ttccattcag gacaaatata tgttcatgta actttttttc ggaaaaaaag ttaccaaaaa
     2761 accttgtcaa aacgtatgct tctgcccgct ctcatcaggt cgggaaaatc atgcaaacga
     2821 gtgtctgaaa aggtcatcaa aggcctacaa caatttgact gattttttga gaaagaaaaa
     2881 aattataaag gttattaata aacataaacg tctgctaaca agctaaatgt tcgtggaaaa
     2941 ggttcgaatt gctgcgtagc agcaacagtg cggtggccag aaaatatggc ggtgt
//
LOCUS       DAOSFP010000298         2987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_31251_length_2987_cov_4.050477, whole genome shotgun sequence.
ACCESSION   DAOSFP010000298 DAOSFP010000000
VERSION     DAOSFP010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31251_length_2987_cov_4.050477"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1521)
                     /locus_tag="PLI16_08455"
     CDS             complement(<1..1521)
                     /locus_tag="PLI16_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84629.1"
                     /translation="MSNGIKIPHPLKREGTYQAERFPEALDYEYIKLDERSLTDLVKQ
                     SAEYARFVKYYNELNLEDGNWQPFFEEIYDYSAKRVKFSSVGELEQKASTSPHLALFL
                     AFLRIFNIALENLNSLTEKHLDFYYRHILQLQSRNETPDRVPVFFETDKHTLQALVPA
                     GTTLSAGKDMNGKPLCYVTENDLIVNKAKVASIKSIYVQRDEKKNMCGVHASADAPVD
                     NAVVENGAAAGWLPFGSSAGKKAGIGFAIASPVLNLKEGKRRITIELHDAALDTAEGL
                     TVEYTSEKGWTEAAIDVNAANAGQKNKLLYLVVKIDPGQPAVLPYNGKIHQAGLDTVH
                     PVVRFIINNDHFADTYHKLSAIRSGQIKNITVQVSGAKNLLIQNDFGIVDNTKPFPPF
                     GANPVKHKSAVYIGNNDIFNKYLQSFNIALSWKGLPGRIDKYYHTYDFALKKLFSKAA
                     DRKKYFDTEQFDEFTPGHPPGKVSILDNGKWKMLKMNKDAGYARDVRYDPARDRSLM"
     gene            complement(1558..1965)
                     /locus_tag="PLI16_08460"
     CDS             complement(1558..1965)
                     /locus_tag="PLI16_08460"
                     /inference="COORDINATES: protein motif:HMM:NF016829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HOH84630.1"
                     /translation="MDDIKSFLGTGWSFPPQFSKTARTVKMIADEADIKNSLEVLLAT
                     RVGERLMQPDYGCNLDVLVFEPVDVTMQTYIKDLVFTAIYLFEPRVKPDDVILSTPEE
                     GQVLIEVFYTIRGTNTRHNLVYPFYLEEGTLLK"
     gene            complement(1988..>2987)
                     /gene="vgrG"
                     /locus_tag="PLI16_08465"
     CDS             complement(1988..>2987)
                     /gene="vgrG"
                     /locus_tag="PLI16_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HOH84631.1"
                     /translation="DSFVQKNNLARINGRVKSFGIPGVQPGDIIELRGVGARFNGRAF
                     VTGVYHAVDGGLWQTDIQFGLNSATFAEKYDNIVERQANGMLPAVNGLQTAKVESLEG
                     DPLGEDRILIFLPVFPEGQNTFWARIATLDAGNRRGTFFRPEIGDEVIVGFIDDNPNN
                     AVILGMMNSSALPAPIATQDANNVKGIYTRSGMKQEFDDEKKSVLIETPQGNKVHLSE
                     QERGIVVEDQNGNKITLDTNGIKLEDMNSNKVTLDSGGMKIQNLAGSEVLLSTSGIKI
                     MSPGTVEIQGAQVKISGASLTLDAAMVKASGVVQSTAVVANSVIGATYTPGAGNVW"
BASE COUNT          729 a          744 c          641 g          873 t
ORIGIN      
        1 catcaacgac ctgtcgcgtg cggggtcata acgaacatcc cttgcatatc cggcatcttt
       61 attcattttc agcatcttcc acttgccgtt gtcaaggata gacaccttcc ccggagggtg
      121 tcccggagta aattcatcaa actgttccgt atcgaaatac tttttccggt cggcagcttt
      181 ggaaaatagt ttcttcagtg caaaatcata ggtatgataa tacttatcga tgcgcccggg
      241 cagtcctttc cagctcagcg cgatattaaa cgattgcagg tatttattaa aaatgtcgtt
      301 attcccgatg tacactgctg atttgtgttt aacggggttg gcgccaaagg gcggaaaagg
      361 cttggtatta tccactatgc caaaatcatt ttgtatcaac agatttttag cgccgctgac
      421 ctgaactgta atatttttta tttgtccgga ccgaatagcc gacagcttgt gatacgtatc
      481 cgcaaaatga tcattattga taataaagcg aacgaccggg tgaaccgtgt ccaggccggc
      541 ctgatgaatt ttcccgttgt agggtaatac agcaggctgt ccgggatcaa ttttcaccac
      601 aaggtacagc agtttgttct tttgtccggc gtttgctgca ttaacgtcaa tggcggcctc
      661 ggtccagcct ttttcagaag tgtactctac ggttaaccct tcggcagtat caagggcggc
      721 atcgtgtaac tcaatggtaa tacgtctttt tccttcttta aggttaagca ccggcgaggc
      781 tatggcaaaa cctatcccgg cctttttgcc ggcagaagag ccaaaaggca gccagccggc
      841 agcagcgcca ttttccacaa cagcattatc tacaggtgca tctgctgaag catgaacacc
      901 gcacatattc tttttttcat ccctttgtac ataaatactc tttatggacg ccacctttgc
      961 cttgttgaca atcaggtcgt tttccgtaac ataacacagg ggtttgccgt tcatatcctt
     1021 gcctgctgac agggttgttc ctgcgggcac caatgcctgg agggtgtgct tatctgtttc
     1081 aaaaaaaacc ggaaccctgt cgggggtttc attacgggac tgcaactgca aaatatggcg
     1141 ataataaaaa tccagatgtt tttcggtcag cgagttcagg ttttccaggg caatattaaa
     1201 tatccttaag aaagccagaa aaagcgccag gtgtggcgat gtggatgctt tttgttccag
     1261 ttctccgacc gaagaaaact tcacgcgttt agcggaataa tcataaattt cttcaaaaaa
     1321 aggttgccag ttgccatctt ccaggttgag ttcgttgtaa tatttcacaa accttgcata
     1381 ttccgccgat tgttttacca ggtcagtgag agagcgttca tcaagtttta tgtactcata
     1441 atccaatgct tcggggaaac gttccgcctg gtaggttcct tctcttttca gaggatgtgg
     1501 tatcttgata ccgttgctca taattatttt tacagttatt ttatttattt catttcttta
     1561 tttcagcagg gtaccttctt cgagataaaa cgggtacacc aggttgtgcc gggtgttggt
     1621 gccacggatg gtataaaaaa cttctatcag cacctgacct tcttcgggtg ttgacaaaat
     1681 cacatcgtca ggttttacac gtggttcaaa tagatagata gcggtaaaaa ccaagtcttt
     1741 gatgtaggtc tgcatggtaa catcaacagg ctcaaaaacg aggacatcca gattgcaccc
     1801 ataatccggc tgcatcagac gctctccgac tctggtagcc agcagcactt ccagactgtt
     1861 ttttatatcg gcttcatccg ctatcatctt cacggtcctg gccgtctttg aaaattgcgg
     1921 cgggaagctc caccctgttc ctaaaaaaga ttttatatca tccataaatt ttaattctcc
     1981 ctgttactta ccaaacattt cctgcgcccg gcgtataggt ggcgcctatt accgagttgg
     2041 caacaaccgc agtggattga actactcccg aagcctttac cattgccgca tccagtgtta
     2101 atgaagcgcc ggaaattttc acctgcgccc cctgaatttc caccgtgccc gggctcatta
     2161 tttttatgcc cgaagttgat aacaacacct cgctgccagc caaattctga attttcatac
     2221 cgcctgaatc gagggtaacc ttattgctgt tcatatcctc aagcttgatt ccgtttgtgt
     2281 ccaaggtaat tttattcccg ttctggtctt cgactacaat accccgctcc tgctcgctca
     2341 agtgaacctt attcccctga ggcgtttcta tcagcacact ctttttttca tcatcaaatt
     2401 cctgtttcat ccctgagcgg gtataaatcc ctttgacgtt attggcatcc tgagttgcta
     2461 tgggtgccgg cagtgcgctg ctgttcatca tcccaagaat tacggcatta ttggggttat
     2521 catctataaa gcctacgatc acctcatcgc caatttcggg acgaaaaaat gttcctcttc
     2581 tgttgccggc atccagggtg gctatccgtg cccagaacgt attttgtcct tcgggaaaga
     2641 cgggcaaaaa aatgagtatt ctgtcttcgc ccagcggatc gccctcaagg ctttctacct
     2701 tggcggtttg caggccgtta accgcgggaa gcatgccatt ggcctgccgt tccacaatat
     2761 tgtcgtattt ttcggcaaat gtggcgctgt tcagaccaaa ctgtatgtcg gtttgccaca
     2821 aaccaccgtc aacggcatga tacactcccg taacgaaggc ccggccgtta aagcgggcgc
     2881 ccacaccacg aagttcaatg atatcaccgg gctgaacacc aggaataccg aatgatttca
     2941 ccctaccgtt gatcctcgcc aaattatttt tttgaacaaa cgaatcc
//
LOCUS       DAOSFP010000299         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_31518_length_2970_cov_4.108062, whole genome shotgun sequence.
ACCESSION   DAOSFP010000299 DAOSFP010000000
VERSION     DAOSFP010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31518_length_2970_cov_4.108062"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLI16_08470"
     CDS             <1..388
                     /locus_tag="PLI16_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84632.1"
                     /translation="IISLFFFKHAASDKLANLSMFVFVFVVGWMAIPYWTNGLYHVFC
                     CGTSSLFDPKSLLPMTIIGEAWRIPILVLYPLFMGYLLFSLIRFIVLVVRRKNPGIIN
                     VVILLFNLLIIAVFFFVPHYFYWLLD"
     gene            complement(414..1856)
                     /locus_tag="PLI16_08475"
     CDS             complement(414..1856)
                     /locus_tag="PLI16_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HOH84633.1"
                     /translation="MKSIREITKQRPWVPWVLFFVTTVIVFLIGLFASSIIERRSESK
                     LYFQVVKEIPEWEPDNAVWGENYPREYDTYIATTDTTFASKHGGGATIDYLQKYPELV
                     VLWAGYAFSSDYKQGRGHYHAIEDITNTLRTNYPQPATCWTCKSTDVPQLMDKMGIAN
                     FYRGKWQDLGKEVVNPIGCQDCHDPKTMNLRITRPALIEAYERSGRDINTATQQEMRS
                     LVCAQCHVEYYFKGEGKYLTFPWDKGQTVDSMEKYYNEVNHIDFTHALSKTPILKAQH
                     PDFEMFQTGIHGQRDVACADCHMPYRREGGIKFTDHKIQSPLNNISGSCQVCHRESEA
                     TLLKNVYDIQDKTEQIRRIAEKNLYIVHVGCKLAYDKGANDDEMKTIHQLIRSSQWHW
                     DWVAAANSMGFHSPVESLRVLALSIQKAQEARLLLLEVLLTHNVKLPFAIPEIATKAE
                     AMKLINLEIDRITGEKAAFLKDVAPTWKKK"
     gene            complement(1861..2472)
                     /gene="nrfH"
                     /locus_tag="PLI16_08480"
     CDS             complement(1861..2472)
                     /gene="nrfH"
                     /locus_tag="PLI16_08480"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HOH84634.1"
                     /translation="MKKILSFFFPPENWKTIVVILLGIFFGLGFFVLYISNAPSYLSD
                     EPKTCINCHVMYPQYASWAHSSHFRATNCNDCHVPHNNFVNKYYFKAKDGLRHSTMFT
                     FRLEPQVIQIKDAGINVVQENCKRCHSDLVSMVSLTEVTGDNHTEGKGHRCWDCHREV
                     PHGTVNSLSANPSALVPHLSPVIPSWLSKTRNKENLQSIQFKK"
     gene            complement(2524..>2970)
                     /locus_tag="PLI16_08485"
     CDS             complement(2524..>2970)
                     /locus_tag="PLI16_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84635.1"
                     /translation="DSIFSELLQACTPSLNNILSSELPLEEKITEYVSKYIDILQENP
                     RLTYFVLSVLQRNPEKIKKLQIFQSLYDTGNFASQFISAMKKRNISSYDPTQFYINMV
                     SLITFPFTIKPVILEKNEMDEKDFNLFMQERKKIITDLLILSIKKK"
BASE COUNT          731 a          628 c          664 g          947 t
ORIGIN      
        1 catcataagc ctttttttct ttaaacatgc cgcatcagac aagcttgcca acctgagcat
       61 gtttgtattc gtatttgtcg ttggctggat ggcgataccc tattggacaa acggtttgta
      121 ccatgttttt tgctgcggca catcatctct tttcgaccca aagtcattgt tgccaatgac
      181 catcatcggg gaagcctggc gaattcccat actggtatta tatccgttgt ttatgggcta
      241 cctgcttttc agcctgatac ggtttattgt acttgttgtc cgccgaaaaa atcccggcat
      301 tatcaacgtg gtgatattac tttttaacct tttaataatt gcggtatttt ttttcgttcc
      361 gcattatttt tattggttac tggattaaaa tttctttttt tcagtgccgg ttattatttc
      421 tttttccagg taggagccac atctttaaga aaagcggctt tttctccggt aatcctgtct
      481 atttcgaggt taatcagctt catggcttct gctttggtag ctatttcggg aatagcaaac
      541 ggcagtttta cgttatgtgt cagcaatacc tcaagtagca gcaggcgggc ttcctgtgcc
      601 ttttggatag acagcgccag cacacgcagc gattccaccg gggaatgaaa tcccatgcta
      661 ttggcagcgg ctacccagtc ccaatgccat tgcgaagaac gtatcagttg atggatggtt
      721 ttcatttcat catcattggc gcccttatcg taagccagtt tgcaacccac atgcactatg
      781 taaaggtttt tttcggcgat acgccttatt tgttcggtct tgtcctgtat atcgtaaaca
      841 tttttcagca gggtggcttc gctttcgcgg tggcatacct ggcatgagcc cgaaatgtta
      901 ttcagcgggc tttgtatttt gtggtcggta aattttatgc caccttccct gcggtaaggc
      961 atgtggcagt cggcgcaggc gacatcgcgc tggccgtgta tgccggtctg aaacatctcg
     1021 aaatcgggat gttgcgcttt taatatcggt gttttgctta gggcatgagt gaaatctata
     1081 tggttcactt cattatagta cttttccatg gaatcaaccg tctgtccctt gtcccaggga
     1141 aaagtcaggt atttgccttc tcctttgaag taatactcca catggcattg tgcacatacc
     1201 agcgaacgca tttcctgttg ggtggcggtg ttaatatccc tgcccgagcg ttcataagct
     1261 tcgatcaggg caggcctcgt gatgcggagg ttcatggttt taggatcgtg acagtcctga
     1321 catccgatag ggtttactac ttcttttccc agatcctgcc atttgcctcg ataaaaattt
     1381 gctattccca ttttgtccat caactgcggc acatcggtgc ttttgcatgt ccagcaggtg
     1441 gccggctgag ggtaatttgt tcgcagggta tttgttatat cctcaatggc atggtagtga
     1501 ccacgccctt gcttgtagtc gctggaaaag gcatatcctg cccatagcac caccagctca
     1561 ggatactttt gcaggtaatc aatggtggct ccgccgccgt gtttgctggc aaaggtagtg
     1621 tcggtggtgg caatataagt atcatactcg cgtgggtaat tttcgcccca cacagcgttg
     1681 tccggctccc attcgggtat ctcttttact acctgaaaat atagcttgga ttcgcttctc
     1741 ctttcgatga tagaagaggc aaaaagacca atgagaaaca cgataacggt agttacaaaa
     1801 aataataccc agggcaccca gggacgttgt ttggttattt cgcgtatgct tttcataaaa
     1861 ttatttttta aattgtatgc tttgcaaatt ttctttgttt cttgttttgc taagccatga
     1921 agggattacc ggcgacaagt gcggtacaag cgctgagggg ttggcgctaa gactgttgac
     1981 ggtgccgtga ggcacttcgc ggtggcaatc ccagcaacga tggcccttgc cttctgtgtg
     2041 gttatctccg gttacttccg tcagtgaaac catactgact aaatcactat ggcatcgttt
     2101 acagttttcc tgaaccacat taatacctgc gtccttgatt tgtatcacct gcggttccag
     2161 ccggaaagtg aacatggtgg aatggcgaag cccgtctttg gctttaaaat aatatttgtt
     2221 cacaaagtta ttatgcggca cgtggcagtc gttgcagttg gtagccctga aatgactgct
     2281 gtgcgcccac gaggcatact gtgggtacat cacatggcag ttgatacatg ttttaggctc
     2341 atccgacagg taggaagggg cgttggatat atacaaaacg aaaaaaccta aaccgaaaaa
     2401 aataccgaga agaattacga caatagtctt ccagttttca ggaggaaaga aaaaactcaa
     2461 tatctttttc aataaataaa tttattgtaa agatatcata aaaatttcaa actgaaatag
     2521 atattacttt tttttaatgc tgagtatcag caggtcggta atgatttttt tacgctcctg
     2581 cataaaaaga ttaaaatctt tttcatccat ttcatttttt tccagaatga ccggttttat
     2641 agtgaatggg aaggtaatga gcgaaaccat attaatataa aactgcgtgg gatcatagct
     2701 gctgatattt ctttttttca tggcactgat aaattggctt gcaaagttgc ctgtgtcata
     2761 taaactttga aaaatctgca gttttttaat cttttcgggg ttacgctgca ataccgacaa
     2821 cacaaaataa gtgaggcgcg gattttcctg tagaatatcg atgtacttac tgacatattc
     2881 tgttattttt tcttccaggg gcagttcgga actcaaaata ttatttaaac tgggagtgca
     2941 cgcctgcaaa agttctgaaa aaattgaatc
//
LOCUS       DAOSFP010000300         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_31521_length_2970_cov_3.803431, whole genome shotgun sequence.
ACCESSION   DAOSFP010000300 DAOSFP010000000
VERSION     DAOSFP010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31521_length_2970_cov_3.803431"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLI16_08490"
     CDS             complement(<1..248)
                     /locus_tag="PLI16_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84636.1"
                     /translation="MQQFKSLTTIIILLLCMQSAFAQGKYTTTNQKAIKLFDKAMESF
                     QAKDMLSCESNLLKAIKEDEQFLEAHMLLGNIYDEQKLT"
     gene            complement(346..972)
                     /locus_tag="PLI16_08495"
     CDS             complement(346..972)
                     /locus_tag="PLI16_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOH84637.1"
                     /translation="MDTNQLLKEGKLLPLMEEFYTLQGEGTHTGKAAWFIRIGGCDVG
                     CRWCDVKESWIAGYHPLTSTDAIIAKAAKCAAKAVVVTGGEPLLCQLDYLCAGLKKEG
                     IKTYLETSGSEKLSGAWDWICLSPKRNAPPLAEIFQLADELKVIVYDDDDFDWAEANA
                     RFVRKECRLLLQPEWSRYKTVIPRIVDYILKHPQWGVSLQSHKFMRIP"
     gene            complement(974..1867)
                     /gene="folD"
                     /locus_tag="PLI16_08500"
     CDS             complement(974..1867)
                     /gene="folD"
                     /locus_tag="PLI16_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOH84638.1"
                     /translation="METIMVLLDGKKISDEIKSEIAAEVSKMIADTGKSPHLAAVLVG
                     EDPASQTYVASKERSCKEAGFISSVYKYPENIPESELLKVVDFLNNDEEVDGFIVQLP
                     LPKHINEQIIIEAINPKKDVDGFHPVNAGRMLLGLPSYLPATPYGIMLLLERYKIETE
                     GKNCVVIGRSHIVGTPMSVLLSRNNKSANCTVTLCHSKTQQLAEICSKADILVAAIGR
                     QGFVTEDMVKPGAVVIDVGIHRIPDETSKSGFRLKGDVKFDEVAKKCSYITPVPGGVG
                     PMTIVGLLMNTLKAAKKEYYK"
     gene            1970..>2970
                     /gene="ffh"
                     /locus_tag="PLI16_08505"
     CDS             1970..>2970
                     /gene="ffh"
                     /locus_tag="PLI16_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005815035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOH84639.1"
                     /translation="MFENLSEKFDRAFKIIKGQGHITDINVAESLKEVRKALLDADVS
                     YKIAKSFTEDVKAKALGQNVLTSVSPGQLMVKIVHDELTLLMGSEKSDINLKGSPAIV
                     LIAGLQGSGKTTFAAKLANYLKTKKGKNPLLVACDVYRPAAIEQLKVLGQQISVEVFS
                     EEEDKKPVSLAKKAINYGKDKGHNVIIIDTAGRLAIDEEMMNEISNIKNAVVPHEILF
                     VVDSMTGQDAVNTAKAFNDRLDFDGVVLTKLDGDTRGGAALTIRSVVNKPVKFIGLGE
                     KMDAIDVFHPERMASRILGMGDIVSLVERAQEQYDVEEAQKLQKKIAKNQFNFNDFI"
BASE COUNT          795 a          670 c          692 g          813 t
ORIGIN      
        1 gtaagttttt gttcgtcgta aatattacca agcaacatgt gtgcttccag aaactgttcg
       61 tcttccttaa tggctttcag caggttggat tcgcagctca gcatgtcttt cgcctgaaag
      121 ctctccatgg ctttatcaaa aagttttatg gctttctgat tggtggtcgt atattttccc
      181 tgggcaaagg cagactgcat gcaaagaagt agaataatta tggttgtcag cgatttgaat
      241 tgttgcatag tatgatgatt tataattata acctgacaaa tgtataatta ttttgcaaat
      301 agcggaccgg aatataaaag tgggttgctc aagcccatgc tgatttcagg gaatcctcat
      361 aaatttatgc gattgcaacg aaacgcccca ttgcgggtgt ttcaaaatgt aatcgacaat
      421 gcgggggata actgtcttat accggctcca ttcgggttgc agcaggagtc tgcattcctt
      481 cctgacgaaa cgggcgttgg cttcagccca gtcaaagtcg tcatcatcgt acactattac
      541 cttcagctca tcggccagtt gaaaaatttc agccagcggc ggcgcattac gtttgggcga
      601 caggcatatc caatcccagg caccgcttag tttttcactg cccgatgttt caagataggt
      661 tttgattcct tcttttttaa gccctgcaca taaataatcg agctggcaga gcaagggctc
      721 tccgccggta accacaacgg ctttggcagc acatttagcg gctttggcaa taatagcatc
      781 cgttgaagtc aggggatgat agcctgcaat ccacgattct ttgacatcgc accagcggca
      841 gcccacatcg cagccgccta tgcggataaa ccaggcagct ttgccggtgt gtgtgccttc
      901 gccctgcaaa gtataaaact cttccatcag cggaagcagt ttaccttctt tcaaaagctg
      961 gttggtatcc aatttatttg tagtattctt tttttgccgc tttcagggta ttcatcagca
     1021 gccccactat ggtcataggg ccaactcccc ccggaacagg cgtaatatat gaacattttt
     1081 ttgccacctc atcaaatttc acatcgcctt tcagacggaa gccggatttg gaggtttcgt
     1141 cggggatcct gtggatgccg acgtcaataa ccacggctcc gggtttaacc atatcctcag
     1201 taacaaaccc ctgcctgccg atggctgcaa ccagaatatc tgccttgctg catatttcgg
     1261 ctaactgctg agtcttgctg tggcaaaggg ttaccgtaca attggccgat ttattgttgc
     1321 gcgacagcag aacgctcatt ggcgtgccaa caatatggct gcgacctatc accacgcagt
     1381 tttttccttc ggtctcaatc ttgtaacgtt ccagcaaaag catgataccg tatggtgttg
     1441 caggcagata agaaggcaat ccgagcaaca tcctgccggc attcaccgga tgaaaaccgt
     1501 caacatcttt tttggggttg atggcttcga tgattatttg ctcgttgata tgttttggca
     1561 aaggcagttg cacgataaaa ccgtcaactt cttcgtcgtt atttaaaaaa tccactacct
     1621 taagcagttc agattcagga atgttttcgg ggtatttata taccgaagaa ataaaacctg
     1681 cttccttgca ggaacgttct tttgaggcca cataggtttg gctggcaggg tcctctccta
     1741 ccagcaccgc tgccagatgc ggcgacttac ctgtgtctgc aatcatcttg ctcacttcag
     1801 cagctatttc ggatttgatc tcgtctgaga ttttttttcc atcaagtaaa accataattg
     1861 tttcaattaa attcacgcaa aggtagttaa tctgccggat aaaaattgaa atcattttgc
     1921 tccgggattt tttttatata tttgccggaa actataatat ttattgcaga tgtttgaaaa
     1981 cctgagcgaa aaattcgacc gcgcgtttaa gattataaaa ggccaaggtc atattacgga
     2041 tatcaatgta gccgagtcgt tgaaagaagt tagaaaagcg ttgctcgatg ccgacgtgag
     2101 ttataaaatt gccaaaagtt ttaccgaaga tgtcaaagcc aaggcgctgg ggcaaaatgt
     2161 gctgacctcc gtgtcgcccg gacaactgat ggtgaagata gtacacgacg aactgacgct
     2221 gctgatgggc agcgaaaagt cggatatcaa cctgaagggc agtcctgcca ttgtgctgat
     2281 agcgggattg cagggttctg gaaaaaccac ttttgccgcc aaactggcca actacctgaa
     2341 aacaaaaaaa ggtaagaatc cgttgctggt ggcttgcgac gtgtaccgtc cggccgctat
     2401 tgaacagtta aaagtgctcg gacagcagat cagtgtggag gtattcagcg aggaagaaga
     2461 taaaaagcct gtttcgttag caaaaaaagc tatcaactat ggtaaagata aaggccataa
     2521 tgttatcatc atcgacaccg ccggacgtct ggctattgat gaagaaatga tgaacgagat
     2581 ctccaatatt aaaaacgccg ttgttcctca tgagattttg tttgtggtgg attccatgac
     2641 aggccaggat gccgtgaaca cagccaaagc ctttaacgac aggcttgatt ttgatggcgt
     2701 ggtgctgacc aaactcgacg gcgacacccg cggcggtgca gccctgacca tcaggtcggt
     2761 ggtgaacaaa cccgtgaaat ttatcggcct tggcgaaaag atggatgcca ttgatgtgtt
     2821 tcatcctgaa cgtatggcca gccgtatcct gggcatgggt gatatcgtgt cactcgttga
     2881 acgtgcacag gagcaatacg atgtggaaga agcccaaaaa cttcagaaaa aaattgccaa
     2941 aaaccagttt aatttcaacg attttatcag
//
LOCUS       DAOSFP010000301         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_31838_length_2950_cov_5.178238, whole genome shotgun sequence.
ACCESSION   DAOSFP010000301 DAOSFP010000000
VERSION     DAOSFP010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31838_length_2950_cov_5.178238"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLI16_08510"
     CDS             complement(<1..784)
                     /locus_tag="PLI16_08510"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOH84640.1"
                     /translation="MYNSYLSCLAGCSTLVVTPQAGHPAYADYQVCGESAIEGCLEGV
                     IFCDTVRVFFLDDVSLSTNPNPAEFCAYESGISITGSIANGLPPYTMYWTDNIDGTGS
                     VIDNDDTYFASVPGTYSFVVQDSRFPGCPQTILNVPVVSHQVPAITAISPTNVSCFGD
                     STGSAATIVTGGTSPYNYIWNDPDAQTSSTAVDLVPGTYTVTVSDAYGCSTTASTVIT
                     QPAGALNAAITAQTNVLCFGNATGSATVTANAGTSPYTYLWST"
     gene            complement(872..1399)
                     /locus_tag="PLI16_08515"
     CDS             complement(872..1399)
                     /locus_tag="PLI16_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84641.1"
                     /translation="MKTTYIPNDLVVEKSKNITQEKSCVYVFNKHLQVFLLVGLLTLF
                     INPDNLYSQSGNCTTNTPFYSVNLVGNPGGIWTSPSFIRQGECCGVLNPDRCLEFEIM
                     LDPNAVGLVFSITSGAIPPGAIYYQINCGPPTPVGTGICLNTPGPHYLTFCKPGNNEN
                     IYSITSISAPFVSPD"
     gene            1804..2142
                     /gene="rbfA"
                     /locus_tag="PLI16_08520"
     CDS             1804..2142
                     /gene="rbfA"
                     /locus_tag="PLI16_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOH84642.1"
                     /translation="MDSIRLNKVSRLVQKELADIFLNQAKKIAGNAMITVTAVKVTKD
                     LSYARVFLSLFGIKDKAALMVSIRESSKEIRRELGNRLKHQLRIIPELDFIHDDSLDY
                     IDHIDELLKH"
     gene            2144..>2950
                     /locus_tag="PLI16_08525"
     CDS             2144..>2950
                     /locus_tag="PLI16_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH84643.1"
                     /translation="MNLPVYIAKRYLFAKKSHNIINIISAISVGGITIGTAALIIVLS
                     VFNGFENMVVTMFNSFNPDLKVTPVVGKTFDEQELDKKNILNVEGISCVTKVIEENAL
                     VKYLDKQYICTVKGVSDGFYRTSRLDSMIVHGDAILERGEQNFAIVGMGVSYNLGLNL
                     NDYENPLSVYVPRRFEGSRINPVEAFNHLLIQPSGVFSVQQEYDQKYIIVPLRFAAEL
                     LESEHQLSALEIKVDPAARPDRVQKQLQSMLGDDYAVKNKYQQEELLFKIM"
BASE COUNT          859 a          559 c          656 g          876 t
ORIGIN      
        1 tagtactcca taaataggta tagggagaag ttccggcgtt ggcggtaacc gtagctgatc
       61 cggtggcatt gccaaaacac aagacattgg tctgtgccgt tatggcggca ttcagtgcac
      121 ctgcgggctg tgtgattact gttgaagctg ttgttgaaca tccgtaggca tcggatactg
      181 tgacggtata tgttcctggc acgaggtcta ctgccgtgct gcttgtctga gcatcaggat
      241 cgttccatat ataattgtaa ggggaagtgc ctccggtaac tattgttgcc gctgaaccag
      301 tcgaatctcc gaaacaggat acattggttg gggatatggc agtaatagcg ggaacttgat
      361 gacttacgac aggtacattc agaatggttt gagggcagcc aggaaacctc gaatcctgga
      421 caacaaaaga ataagttccc ggtactgaag caaagtaggt gtcatcattg tcaatcactg
      481 agccagttcc gtcgatgtta tctgtccaat acatggtata ggggggtaag ccgttagcta
      541 tgcttccagt tatactaata ccgctttcat aagcacaaaa ttcagccgga tttggatttg
      601 ttgataagct gacatcgtca agaaaaaata ctctgacagt atcgcagaaa ataacaccct
      661 ccaggcatcc ttcaatagcc gattctccgc atacctggta atctgcataa gcagggtggc
      721 ctgcctgggg agtgacaact aatgttgaac accctgcaag acaacttaaa taggaattgt
      781 acaaaccatt tcctgtaata tcgttccaga ttattgttgc cggatcaaag cctgttgcat
      841 taaccgttgc cggacatcca gcagaggcgt attaatcggg agatacaaag ggggctgaaa
      901 tagatgttat ggaatagatg ttttcattat tgcctggttt gcaaaacgtg aggtaatgtg
      961 gcccaggggt gtttaaacaa atgcctgtcc ccactggcgt tggaggtccg caatttatct
     1021 gataataaat cgcgcccggg ggtatagccc cggaagtaat gctgaaaact aatccgacag
     1081 cgttgggatc aagcatgatt tcaaactcca gacaccggtc cgggtttaaa actccgcaac
     1141 attccccttg tctgataaat gaaggggatg tccatattcc tccgggattt ccaaccagat
     1201 ttacagaata gaaaggagtg tttgtggtac aattaccgga ttgtgaatag aggttgtccg
     1261 gattaataaa taaagtaagc agaccaacta acaaaaaaac ctgtaagtgt ttattgaaca
     1321 cataaacaca tgatttttcc tgtgttatgt ttttggattt ttctacgact aaatcattgg
     1381 ggatatatgt tgttttcata ggcttctgta gttgatggtt tagcgcaaaa attttatctg
     1441 cttttttatt tatgtcatct ttatttggtt gtcagttatt ttttttaaat attgacaggg
     1501 ttataatcag gtgttatggg ttttgtcgtt attattctta tttatgtctt gaaatattgt
     1561 agaattgtta ttcttttttg aaaaatgatt attgaaattc gttttttttc tttaaagcgc
     1621 aagtataaac aattttcctt aaaaaagcaa gtttttatta aaaaaaagtt cagcatagcg
     1681 gtctagtatt ttcatatatc gttgaactca agtcgtatat attgagtgga catatgggtc
     1741 ttatctggca tataaaaagt ggttctattc cgaaatcggg aaataattct tatctttgca
     1801 ttcatggata gcataagact caataaagtt tcacggctgg ttcagaaaga actggccgac
     1861 atttttctaa atcaggcaaa aaaaattgct ggtaacgcaa tgattactgt gacagctgta
     1921 aaggtgacca aagatttatc ctatgccagg gtcttcctta gtctttttgg tatcaaggac
     1981 aaagcagcac taatggtttc catcagggaa agcagcaaag aaatcagacg cgaactgggc
     2041 aacaggctca aacatcagct gcgcatcatt cccgaactgg attttattca tgacgactcg
     2101 ctggactaca ttgaccatat tgacgaactg ctgaaacact aggttgaact tacctgttta
     2161 catagcaaag cgttatcttt ttgcgaaaaa atcgcacaat attattaata ttatttctgc
     2221 tatttcagtt ggtggaatta ccattggcac agcagcatta attattgtcc tttctgtttt
     2281 caatggtttt gaaaacatgg tggttaccat gttcaattcc tttaacccag acctgaaagt
     2341 gactcccgtc gtgggaaaga cttttgatga gcaggaactt gataaaaaaa atattttaaa
     2401 tgtagagggc atcagttgtg ttaccaaagt cattgaagaa aatgcccttg tcaaatacct
     2461 tgataagcaa tacatctgta ccgtaaaagg tgtgagtgat ggtttttacc gcaccagcag
     2521 gctggattcc atgattgtgc atggagatgc aattcttgaa cggggtgagc aaaattttgc
     2581 catcgtagga atgggcgttt catataatct tggcctaaac ctaaatgact atgaaaatcc
     2641 gttgtcggtt tatgtgccgc gtcgtttcga ggggagccgc ataaacccgg tagaggcttt
     2701 caaccacctg ttgatccaac cctcgggtgt gttttcggta cagcaggaat acgaccaaaa
     2761 atacataatt gtacccctgc gttttgctgc agagctcctc gaatctgaac accagttatc
     2821 tgctctggaa ataaaagtgg atcctgctgc caggcccgac agggttcaaa aacaactgca
     2881 atcaatgctg ggcgatgact atgctgtcaa aaacaaatac cagcaggaag aactgctttt
     2941 taagataatg
//
LOCUS       DAOSFP010000302         2934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32100_length_2934_cov_2.496700, whole genome shotgun sequence.
ACCESSION   DAOSFP010000302 DAOSFP010000000
VERSION     DAOSFP010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32100_length_2934_cov_2.496700"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLI16_08530"
     CDS             <1..842
                     /locus_tag="PLI16_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOH84644.1"
                     /translation="FTLILLFTNSYKKIEKWIIAFVSIVGLSFIYELYLVDINWPSVA
                     ICFFKPEIPDHSIVFIMGVLGAVIMPHNLFLHSEVIQSRQWNLREEKVIRKQLRYEFT
                     DTLLSMVIGWAINCAIILLAASTFFHNGVAVDGLEQAKSILEPLLGSSSAVIFAVALL
                     FSGIASGITSGMAGGTIFSGVFQEPYDIKDNHSRMGVLISIVLAVIIIMFVANPFLAL
                     IYSQVALSIALPFTIFIQIYLTSSKKVMGKYANGMFLKVVLFALAAVVTGLNIYLLID
                     TLF"
     gene            943..1389
                     /gene="moaC"
                     /locus_tag="PLI16_08535"
     CDS             943..1389
                     /gene="moaC"
                     /locus_tag="PLI16_08535"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOH84645.1"
                     /translation="MPKLSHTDSSGRANMVDVGHKPQQTRIAKASGMIMLHKDTLKLI
                     KENVVKKGDVLTVAEVAGIQAAKQNAFIIPLCHPLLLTKIDVKCTLRKNGVEAESVVK
                     CIGQTGVEMEALTAVSVALLTVYDMCKAMDKEMVISDIRLLSKEKI"
     gene            1481..2611
                     /locus_tag="PLI16_08540"
     CDS             1481..2611
                     /locus_tag="PLI16_08540"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH84646.1"
                     /translation="MKIVKKIVLVILALIVLINLGIVVSGNSYIYKALSYTYVGIDDY
                     TLFENRPVKAGKPIPIPEAAQYNKTKLSPELKKYLVETQTIAFLVLKNDSVYYEEYWD
                     GYDANSLSNSFSAAKSIVSILTGIAIDEGKIKSVDQKVSDFIPEYKHGFNADLTVKHL
                     LTMTASFDWDESYEGLFSKTTKAYYGRNLKKMLLDFEVVETPGKTFKYQSSNQLVLTY
                     ILEKATGMTLSEYASEKLWKPLGARNDALWSLDKNDGMEKAYCCFNSNARDFSRIGLL
                     YLHNGVFNGTRIVSEEYVKKSLEPAGVTDEDGNTVDYYGYSWWLSDVDTVNYFYARGI
                     LGQYIIVVPDKNIVVVRLGKIRKGHKGLPPHDYITTCVIKEF"
     gene            2620..>2934
                     /locus_tag="PLI16_08545"
     CDS             2620..>2934
                     /locus_tag="PLI16_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84647.1"
                     /translation="MKIKIVSVNISEKKGTIKHPVEKVIISASGVENDAHSGDWHRQV
                     SMLSTQSIAKSEEKAKRKINFGEFAENLTISGMNLVATAPLDRFVINDVELEVTQIGK
                     KCH"
BASE COUNT          861 a          554 c          678 g          841 t
ORIGIN      
        1 tgtttaccct gatattgttg tttaccaact cttataaaaa aatcgaaaaa tggattattg
       61 ctttcgtttc tattgtgggt ctttcgttca tttatgagtt ataccttgtt gacataaact
      121 ggccttctgt cgccatatgt tttttcaaac ccgaaatacc tgaccattcc atagttttta
      181 tcatgggtgt tttgggcgct gttatcatgc cgcataattt gtttctgcac tccgaggtaa
      241 ttcaaagccg ccagtggaac ctccgggagg aaaaggtaat cagaaaacag ctcagatatg
      301 agtttaccga caccctgctt tccatggtaa ttgggtgggc aatcaactgc gcaataattt
      361 tgcttgccgc atcaactttt tttcataacg gagttgccgt tgacgggttg gagcaggcaa
      421 aaagcatatt ggaacctttg cttggcagca gttctgctgt gatttttgct gttgctttgt
      481 tgttttcggg tattgcttcg ggtatcactt cgggaatggc cggcggcaca atattttcgg
      541 gtgtctttca ggaaccgtac gacatcaaag acaaccattc ccgtatgggt gtgctgatct
      601 ctattgtgct tgcggtaatt atcatcatgt ttgtagccaa tccgttcttg gcgctcattt
      661 attcgcaggt ggccctgagt attgccttgc cgtttaccat ttttatccag atttatctta
      721 cctcatcaaa aaaagtaatg ggaaaatatg ccaatggtat gtttctgaaa gtggtgttgt
      781 ttgccctggc agctgttgtt acaggcctga acatttatct gttgattgat actttgttct
      841 gaaatgcgac ggggagagga tgctattttt tatggctcgt tatgctgcga aaaccctgaa
      901 aagtataaat aaaagtagta attttgagtt taaaacgaca gtatgcccaa actatctcat
      961 accgattcat caggaagggc caatatggtg gatgtaggcc ataaaccgca acaaacccgc
     1021 attgccaaag caagcggaat gataatgctg cacaaggata cgctaaaatt gataaaggaa
     1081 aatgtggtga agaaaggtga tgtgctgacc gtggccgagg tggccggcat acaagccgcc
     1141 aagcaaaatg ccttcattat tcccttgtgc cacccgttgt tgctgacaaa gatcgatgta
     1201 aaatgtaccc tgcggaaaaa tggggtggag gccgaaagtg ttgtgaaatg tatcgggcaa
     1261 acaggagtgg aaatggaagc cctcacggct gtgagcgtcg cactgctgac tgtgtatgat
     1321 atgtgcaagg ccatggataa ggaaatggta attagcgata tcaggctgtt gagcaaagaa
     1381 aagatttgag aactgtgtac acgaaaaatg ggaaaataga ggtattcgga agtaggctgt
     1441 gttcctgaaa ttagtcaaca tagtttagta atggaaaaag atgaaaattg taaagaaaat
     1501 agtactggta attctggcat taatcgtgtt gattaacctg ggaattgtcg tttcgggcaa
     1561 tagttatatc tataaggcct tgagttatac ctatgtcggg attgatgatt acacactgtt
     1621 tgaaaaccgc cctgtgaaag ccggaaaacc tatccccatt cccgaagctg cacaatataa
     1681 caaaacaaaa ctttcaccgg aattaaaaaa atatcttgta gaaacacaaa ccatagcttt
     1741 tctggtgctg aaaaatgatt ctgtgtatta tgaagaatac tgggatggtt atgatgccaa
     1801 ctcgctttct aattcgtttt cagcggctaa aagcattgtc agcatcctga caggtattgc
     1861 aatcgatgaa ggaaaaatta aaagcgttga tcagaaagtg tcggacttca ttcctgaata
     1921 taagcatgga tttaatgccg accttacagt taaacacttg ctcaccatga cagcttcctt
     1981 tgactgggat gagagttacg agggcctgtt ttcaaaaacc accaaagctt attatggccg
     2041 taatctgaaa aaaatgttgc tcgattttga ggtcgtcgaa acgcccggaa aaactttcaa
     2101 atatcaaagc tcgaaccagc ttgtgctgac ctatattctg gaaaaggcta cgggtatgac
     2161 tttgagcgaa tatgcttcag agaaattgtg gaaacctctg ggtgcccgta atgatgcact
     2221 ttggagcctt gacaaaaatg acggcatgga aaaagcttat tgttgcttca actccaatgc
     2281 acgtgatttt tccagaatcg gcctgcttta tcttcacaac ggcgtattca atggaacccg
     2341 cattgtttct gaggaatatg tgaagaaatc cctggagccg gcaggcgtaa ccgatgaaga
     2401 tggcaatacg gtggattatt acggctattc ctggtggtta tctgacgtgg atacagtcaa
     2461 ctatttttat gccaggggga ttttagggca atatattatt gttgttcctg acaaaaatat
     2521 cgtggtcgta aggctgggaa aaatccgcaa aggccataag ggcttgcctc cgcacgatta
     2581 tatcacaact tgtgttatca aagaatttta aaaatttcca tgaaaataaa aattgtatca
     2641 gtaaatattt ccgaaaaaaa aggtacgata aaacaccctg ttgaaaaagt tattatcagc
     2701 gcttccggtg ttgaaaacga tgctcattcc ggcgactggc acaggcaggt cagcatgttg
     2761 tccacccaaa gtattgcaaa gtctgaggaa aaagcaaagc gaaaaatcaa ttttggcgag
     2821 tttgccgaaa accttaccat ttcgggtatg aacctggtgg caacagctcc gctcgacagg
     2881 tttgttatta acgatgtgga actggaggta acgcaaatcg gtaaaaaatg tcat
//
LOCUS       DAOSFP010000303         2933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32107_length_2933_cov_4.349548, whole genome shotgun sequence.
ACCESSION   DAOSFP010000303 DAOSFP010000000
VERSION     DAOSFP010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32107_length_2933_cov_4.349548"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="PLI16_08550"
     CDS             <1..761
                     /locus_tag="PLI16_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84648.1"
                     /translation="APYYEISKNIIANELFWNKSFTGFRFPFTKSSFLGLECLSGRRY
                     KFDSSIGVDNLESFYGCAFPYEICISGNGLYKYSGLLEISPILNDDYFFFEQILNGAY
                     SEDKLTKDAKLYQKYLLNTWEYAIKPYNGLMVYLGHPLYVGHSDSTMIPLINLVKQVK
                     SENTWITTIEAVADYWEKLGQTRFFVNENDESAIITVKTADSITIKNISFEAYKKPTA
                     IEARIGKASIVKKDNTNFIVFDAAPNQQVTVRFK"
     gene            complement(793..867)
                     /locus_tag="PLI16_08555"
     tRNA            complement(793..867)
                     /locus_tag="PLI16_08555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(832..834),aa:Glu,seq:ctc)
     gene            complement(1007..2497)
                     /locus_tag="PLI16_08560"
     CDS             complement(1007..2497)
                     /locus_tag="PLI16_08560"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOH84649.1"
                     /translation="MRKAIILIISIFIFQMTKASPGDSIFNMTYIHNLHITFPYPNFF
                     DSLIYTNITDEYIPVDIDFNGEIYSQVGIKVKGNSSFNNPSQKKSFKLDFNTFVQGQD
                     MHGLKKLNFNNSFKDPSFMREKLNNDFLISHDLPAPRVTYCNVYMNNQLWGLYAIVEE
                     IDDEFCTRWFNSNDGNLFKGDPHGTLQWKGSMNQPLYESDYELKNNSSVNDWSDLISL
                     INVINNTPDASLPATLDQKMHTEDFIRHWVAENIFVNLDSYLGSGHNYYIYHDTVTDK
                     FQWIAWDSNESFGSFKMNLSTSQLKNLDMYFVGQAGARPLCENLLENSHYKNLYDMAY
                     CRLRDDFSNLYFDPIIDSLRPVIQSSVYSDPKKFYNNAKFDSSFNYDVKLPEGIVVFG
                     LKPFIAARSVAIQNSITGHGVDCSLQVSEGISPLHGINVYPNPASAVLYIEAGIPIEK
                     TTLYDLQGRLVRTYCITAGFIDISDVSPGMYVLRINDGLYGKIIIN"
     gene            complement(2594..2665)
                     /locus_tag="PLI16_08565"
     tRNA            complement(2594..2665)
                     /locus_tag="PLI16_08565"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2630..2632),aa:Arg,seq:cct)
BASE COUNT          906 a          586 c          543 g          898 t
ORIGIN      
        1 ctgcacccta ttacgaaatc agtaaaaaca ttattgctaa cgaattattc tggaataaat
       61 cttttacggg atttcggttt ccgtttacaa aaagcagttt tctgggatta gaatgtttat
      121 cgggacgcag atacaaattc gattccagca taggtgtgga caaccttgaa agtttttatg
      181 gctgcgcatt cccctatgaa atatgtattt cgggcaacgg cctgtacaaa tattcgggtc
      241 tgttggaaat ttcccccatt ttgaatgatg attacttttt ctttgaacaa atactcaatg
      301 gagcctattc tgaagacaag ctgacaaagg atgccaaact ttatcagaag tatttactca
      361 acacctggga atatgccata aagccatata atggacttat ggtttacctg ggacatcctc
      421 tatatgtcgg acatagtgat tcaaccatga tacccctgat aaacttagta aaacaagtta
      481 aatcggaaaa cacctggata accaccattg aagccgttgc ggactattgg gaaaagcttg
      541 gacaaacacg tttttttgtg aacgaaaatg atgaatcagc tattattacc gtaaaaaccg
      601 ccgacagtat taccataaaa aatatttctt tcgaggcgta taaaaaacca acggccattg
      661 aagccaggat aggcaaggcc tccattgtga aaaaagacaa tacaaacttt attgtttttg
      721 atgctgcacc caatcagcag gtaacagtaa ggttcaaata attttgaatg ggtcgttttt
      781 ttactgtaaa agtggcagcc ccgatgagaa tcgaactcat gtttttagac tgagaatcta
      841 ttttcctaac cactagaaga cggggccgag tggtattata aactatttca gataactaat
      901 cagggacaaa attatgatat ttttttggtt tcagacaatt cattggtttt tttataccga
      961 ttgcacattg gtaatacacc aacaaatacc tggaaaattt tgaaaattag ttaataatta
     1021 ttttcccgta caggccatcg tttatcctaa gcacatacat acccgggcta acgtcgctga
     1081 tatcaataaa tccggcggtt atgcagtaag tccgcaccaa tcgtccctga agatcataaa
     1141 gtgttgtttt ttcaatgggg attcccgctt caatatacaa cacggctgac gcagggttgg
     1201 gataaacatt tatcccgtgc aacgggctta tgccttcaga cacctgcagc gaacaatcca
     1261 caccatgacc ggtaatactg ttctgaatag ccacactgcg agctgcaata aacggtttta
     1321 acccaaaaac aacaatacct tccggcaact tcacatcata gttaaaactt gaatcaaact
     1381 tggcattgtt ataaaatttt ttcgggtcgg aatacacgct gctttgaatt accggccgca
     1441 acgagtcaat gattggatcg aaatataaat tggaaaaatc atcccttagc cggcagtaag
     1501 ccatatcata aaggttttta taatgggagt tttctaacag attttcacaa agtggcctgg
     1561 caccggcctg cccaacaaaa tacatatcga gatttttcaa ttggctggta ctgaggttca
     1621 ttttgaagct tccaaatgat tcgttgctgt cccacgcaat ccactgaaac ttatcagtaa
     1681 ccgtatcatg ataaatatag taattatgtc cactgcccag gtaggaatca aggtttacaa
     1741 aaatattttc cgccacccag tgtcgtataa aatcttccgt gtgcatcttt tgatccaagg
     1801 ttgcaggcaa actggcatca ggcgtattat tgatcacatt gatcagggat atcaggtcgc
     1861 tccagtcgtt tacagagctg ttgtttttaa gttcataatc ggattcatac aacggctggt
     1921 tcatgcttcc tttccattgc agtgtgccat gggggtcgcc tttaaacagg ttgccatcat
     1981 tgctattaaa ccatcgtgta caaaattcat catcaatctc ttccacaatg gcatataatc
     2041 cccaaagctg gttgttcatg taaacattac agtaggtaac ccgcggggct ggaaggtcgt
     2101 gactaatcag aaaatcatta ttgagctttt cgcgcatgaa tgatggatct ttgaaagaat
     2161 tgttgaaatt aagtttttta agcccgtgca tatcctgtcc ctggacgaaa gtattaaaat
     2221 caagcttaaa agattttttc tggcttgggt tattgaatga tgaattgcct tttactttaa
     2281 tgcccacctg actgtatatt tctccgttaa agtctatatc aacaggaata tattcatcgg
     2341 taatattcgt ataaatcagg gaatcgaaaa aattcggata aggaaaagtg atatgaagat
     2401 tatggatgta cgtcatatta aaaatcgaat ctccggggct ggccttagtc atctgaaaga
     2461 tgaatattga aataatcagg ataatggctt ttctcataac tttgatttag taatggattt
     2521 ttgtgattta tcgtttttct ctttatcaaa tttacaaaaa aaaagcgaca cctgttgtat
     2581 cactttattt ccgtagcccc gacaagaatc gaacttgtat ttaaagttta ggaaacttcc
     2641 gttctatcca ttgaactacg gggccaaaac ttttgcaaag gtaaaatttt ttacggatat
     2701 ccgtccatcc ttcagacttt ctaatgcgta aacaaaaatc ttttgaatcc ttttttatgt
     2761 ttacttccgt tccctgcctg ccagcaggca ggtatccatc gaactaaggg gccaattttt
     2821 ttcaaaaata atttatttca ccacacaacc gttgtgaaaa tcttccgaag gacttacaaa
     2881 acataatttg ccttcgctgt tttcagccat cagtatcata ccctgcgaag gta
//
LOCUS       DAOSFP010000304         2929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32178_length_2929_cov_3.884482, whole genome shotgun sequence.
ACCESSION   DAOSFP010000304 DAOSFP010000000
VERSION     DAOSFP010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32178_length_2929_cov_3.884482"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..715
                     /gene="ruvC"
                     /locus_tag="PLI16_08570"
     CDS             185..715
                     /gene="ruvC"
                     /locus_tag="PLI16_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOH84650.1"
                     /translation="MGIDPGTNILGYGLIHNKDKKIELITMSVIKLNKLNDPYLKLKT
                     IFEEVLGIIEQYKPDELAIEAPFFGKNVQSMLKLGRAQGVAIAAALYKSIPVFEYSPR
                     KIKQAITGKGSASKEQVASMLARIIPINVVTEYYDATDALGVAVCHYFQKNTLSDKKA
                     YSGWKSFVSNNPERIK"
     gene            717..1808
                     /locus_tag="PLI16_08575"
     CDS             717..1808
                     /locus_tag="PLI16_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOH84651.1"
                     /translation="MTAVFNPTACVVNHRIFSETLKFYSELIADIENAKNNICFEIFK
                     FIDDAVGIRIRDALIKKSREGVRVRLMIDSWGVPSSDTFFDELVSAGGQVRFFMKLRY
                     NLDALTKHHRRNHRKIIVIDDHIVYLGSANINQYSITWRELIVKMEGKIAHTFKKAFN
                     SSWKIYRKNTFNIRHQTTALSCGDFKIIRDVPSLTRQKVKKKFESLIKNARRQVIIET
                     PYFLPGYYLRKLLMDAGKRGVDVKVIIPKQSDVTLVDILRNKYLGLLHKNNVQLLMYK
                     PNNLHSKMMLVDNEAFFFGTSNFDYRSFRYMYEIMLLGKDPNLLQDLVVHNNKTLENC
                     DTFNYELWKKRPMMQKFFENLLIPFRHLL"
     gene            complement(1812..2375)
                     /locus_tag="PLI16_08580"
     CDS             complement(1812..2375)
                     /locus_tag="PLI16_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOH84652.1"
                     /translation="MRRYQIFLLCFILSGMVVFSSCKTTRHSRPITHKESKNPDNADT
                     GLLKDYAGKFGVNFNGDEDLNLVSCLAGWLNVPYKYGGNTKQGTDCSGLVSSVFKEVY
                     NIAMYRSSADQLKNVREISKSQLKAGDLVFFKIDNNKVSHVGIYLGGNKFIHATTQKG
                     VIVNSLDEEYYKKYYYTAGRVIMQGLP"
     gene            complement(2458..2841)
                     /locus_tag="PLI16_08585"
     CDS             complement(2458..2841)
                     /locus_tag="PLI16_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOH84653.1"
                     /translation="MNSFFENDSLNFFNRQHCSVPAANIVENENSFELELAVPGMNKE
                     DFRIDVENNMLTVSSEKKEENEESNKNYTRKEFVFGSFSRSFMLPKSIDNDKINAEYK
                     NGVLSLHLPKREEEKTKVKRQIAIS"
BASE COUNT          894 a          614 c          565 g          856 t
ORIGIN      
        1 ggctccgaca ctatcatcaa caagccatta ctgaggcctg caagatcttc tttgcaaaat
       61 aaacgatatc caataaagcc aaaagaaaga aatattatca gaattttgat aatataatta
      121 gccgttttga ttaattttgc tttaggcatt taatcttatt tttgtgagta cggacagaat
      181 aattatgggt atcgacccgg gaacaaatat tttgggttat ggattaattc ataacaaaga
      241 taaaaagatt gaacttatca cgatgagcgt gattaaatta aacaagctca acgatccgta
      301 cctgaagctg aaaaccatat ttgaagaggt tctgggcatc atagaacagt ataagcccga
      361 tgaactggcc attgaggcgc catttttcgg aaaaaatgtg caatcgatgc tcaaactcgg
      421 ccgtgcacag ggagtagcca ttgctgcagc attgtacaaa tcgataccgg tgttcgaata
      481 ctcgccacgt aaaataaaac aggccattac cggtaagggc agcgcttcca aagaacaggt
      541 tgcctcgatg ttggccagga taatccccat caatgtggtt accgaatact atgacgccac
      601 cgatgcattg ggggtggctg tttgccatta ttttcaaaaa aacaccctta gcgataaaaa
      661 agcctatagc ggctggaaga gttttgtcag taataaccct gaaagaataa aataatatga
      721 ccgctgtttt caaccccacg gcatgtgttg taaatcacag aattttcagc gagaccctta
      781 agttttattc tgagttgatt gccgacattg aaaatgccaa aaacaacatt tgttttgaaa
      841 ttttcaaatt tattgatgat gctgttggta taagaatcag ggatgcactg ataaaaaaaa
      901 gccgtgaagg tgtccgggta agattaatga tagattcatg gggagttcct tccagcgaca
      961 ctttttttga tgaacttgtc agcgccggcg gccaggtaag atttttcatg aaactccgct
     1021 acaacctcga tgcgctgaca aaacaccacc gccgcaacca ccggaaaatc attgtgattg
     1081 acgaccatat tgtttatctt ggatcagcca atatcaacca atattccatt acctggcgcg
     1141 aactgatagt aaaaatggaa ggcaaaattg cacatacttt taaaaaagcg ttcaacagca
     1201 gttggaaaat ttacaggaaa aacacgttca acatcaggca tcagaccact gcgctcagtt
     1261 gcggagattt taagattatc cgcgatgtgc cctcgcttac caggcaaaag gtgaaaaaga
     1321 aatttgaaag cctgataaaa aatgcccgcc ggcaggtaat catcgaaaca ccttattttc
     1381 tgccgggcta ctacctgaga aaattgctta tggatgccgg caaaagaggc gtagatgtaa
     1441 aagtcatcat acccaaacaa tcggatgtaa ccctggtgga catcctgaga aacaaatacc
     1501 tgggattgct gcataaaaat aatgtacaat tgctgatgta caaacccaac aacctgcact
     1561 ccaagatgat gctggtggac aatgaagcat tttttttcgg cacctccaat tttgattacc
     1621 gcagtttcag gtatatgtat gaaattatgc ttctgggaaa agaccccaat ttactgcaag
     1681 acctggtggt gcacaacaac aaaaccctgg aaaattgcga cacgtttaat tacgagctgt
     1741 ggaaaaaacg gcccatgatg caaaaattct tcgaaaacct gctgataccc ttccgccacc
     1801 ttttatgatt atcagggcaa gccttgcata atgacccttc cggcggtata atagtatttt
     1861 ttataatatt cttcgtccag gctattaaca attacgcctt tttgagtggt agcatgaatg
     1921 aatttgttcc cgccgagata aataccaaca tgcgacacct tgttattatc aattttaaaa
     1981 aaaacaaggt cacctgcctt caactggctt ttgcttattt ccctgacatt tttaagctga
     2041 tcggccgaac tcctgtacat ggcaatatta taaacctcct taaataccga acttactaat
     2101 cccgagcaat ctgttccctg tttggtgttc cctccgtatt tgtaaggaac gttcagccag
     2161 cctgccaggc atgaaaccag gtttagatct tcatcgccgt tgaaatttac accaaacttc
     2221 ccggcataat ccttcagcaa accggtatct gcattatctg ggttcttgct ttccttatga
     2281 gttattggcc tgctgtgccg ggtagttttg cacgaagaaa acaccaccat ccccgataaa
     2341 atgaagcaga gcaaaaaaat ctggtagcgt ctcattatca atattttata ttgcaaaaat
     2401 ataaataaaa aaaggagata gccgggctac ctccttttat ttgccaacaa cactttgtta
     2461 actaatagca atctgccttt ttactttggt cttttcttct tctctcttag gcagatgcag
     2521 gctaagaaca ccatttttgt actcggcgtt gattttatca ttgtcaatgc ttttgggcaa
     2581 cataaacgag cggctgaagc ttccgaaaac aaattccttg cgggtgtagt ttttgttgct
     2641 ttcttcattt tcttccttct tttctgaaga aactgtcagc atgttgtttt ccacatctat
     2701 tctgaaatct tctttgttca tgccgggcac agccaactcg agttcaaaag aattttcgtt
     2761 ttcgacaatg ttggccgcag gaacgctgca atgttgcctg ttgaaaaaat tcaacgaatc
     2821 gttttcaaaa aaactgttca ttaaatctga gaatgttggc tcgttgttta atctgataag
     2881 ttttatggtt ttatcctcct gtttttatgt ttataaattt ttaagtttt
//
LOCUS       DAOSFP010000305         2928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32198_length_2928_cov_3.633136, whole genome shotgun sequence.
ACCESSION   DAOSFP010000305 DAOSFP010000000
VERSION     DAOSFP010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32198_length_2928_cov_3.633136"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLI16_08590"
     CDS             complement(<1..1181)
                     /locus_tag="PLI16_08590"
                     /inference="COORDINATES: protein motif:HMM:NF020249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HOH84654.1"
                     /translation="MTKVKILWVDDEIDLLKPHILFLQEKGYAVHTSNNGDEALDQIR
                     DGDFDIIFLDENMPGLTGLETLAQIRNDYPNLPVVMITRNEQEHTMEDAIGSNISDYL
                     IKPVNPNQILLCLKKNLENKRIISEKTTQAYQQQFRHIGMEISNRLSHTEWMEVYKKM
                     VFWELELEKSNDESMNEVLALQKSEANAMFSKFYENNYIAWLSGKAADTPVMSHNLIK
                     EKVFPALNLPENLFVIVIDNLRYDQWKVIQPVLEEFFRVQNDEMYYSILPSATQYARN
                     ALFAGLLPTEIERKYPKYWTYEEDEGTKNQYENELLGELLRRYGKDIRYNYSKILNIN
                     AGKKYVENLPNLMQNKLNVLVYNFVDMLSHARTDMEIIRELASDEAAYRSITLSWLKH
                     SP"
     gene            1274..2503
                     /locus_tag="PLI16_08595"
     CDS             1274..2503
                     /locus_tag="PLI16_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH84655.1"
                     /translation="MVHNKRKIINDPVHGFITIPSELVFDIIENPYFQRLRHIKQLGL
                     TYLVYPGAIHTRFQHAVGALHLMTLAVEMLKVKGTEITDEEIQGLYLAILLHDIGHGP
                     FSHALEHTLVEEVSHETLSLLFFRRLNETFGGRLDMAIRIFSDQYPKRFLHQLVSGQL
                     DVDRLDYLMRDSFFTGVSEGVISTDRIIKMMNVYNDELVVDVKGVYSIENFIVARRLM
                     YWQVYLHKTVLSAEHLLIHILKRAKFLCEQGKTIFAPPALQEFLKNRFTIADFNENPG
                     LLDSFAQLDDYDVFMSVKMWTRSDDRVLSLLSKKLVERKLLRIEISDTPFDEGYIDSL
                     KQKVQQAFDISAEEADYFVFSEEVANNAYNPYFDKIHVLYKDNRVLDITESSDQLNIN
                     LLTKTVRKYFLCYPKGL"
     gene            complement(2707..>2928)
                     /locus_tag="PLI16_08600"
     CDS             complement(2707..>2928)
                     /locus_tag="PLI16_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84656.1"
                     /translation="QKIFENLQRPRRLRGFPTRAVGTTLIRFSIFSESGLKHCLGCLF
                     PCLPLPSGQAGLVHFWRLPRLLSGDKQKK"
BASE COUNT          763 a          624 c          710 g          831 t
ORIGIN      
        1 ggcgagtgtt tcaaccacga cagggtgatg gaacggtaag cagcttcgtc gctggcaagt
       61 tcgcgtatga tctccatatc ggtacgggca tgcgaaagca tgtccacaaa gttgtaaacc
      121 aatacgttga gtttgttctg catcaggttg ggcaggtttt caacatattt tttgccggca
      181 ttgatattca atatttttga ataattgtag cggatatctt tgccatagcg gcgtagcaac
      241 tcgcccagca actcgttttc atactgattt ttcgtgcctt cgtcttcttc gtaggtccag
      301 tattttgggt atttgcgttc aatttcggta ggcaacaggc cggcaaaaag agcatttcgg
      361 gcatattgcg ttgctgaagg caggatgctg taatacattt cgtcgttctg caccctgaaa
      421 aactcttcga gcacaggttg gatgaccttc cattggtcat agcggaggtt gtcaatcaca
      481 atgacaaaaa ggttttcggg caggtttaac gcgggaaaca ccttttcctt tatgaggttg
      541 tgcgacatca cgggcgtatc ggcggctttg ccgctcagcc aggcgatata attgttttca
      601 taaaattttg aaaacatggc gttggcctcg gatttttgaa gcgcaagcac ctcgttcatc
      661 gattcgtcgt tagacttttc gagttctaat tcccaaaaca ccattttttt atatacttcc
      721 atccattcgg tgtgcgacag gcggttagaa atttccatgc cgatgtggcg gaattgctgc
      781 tggtaggcct gggtggtttt ctcgctgatg atacgtttgt tttcgagatt ttttttcagg
      841 cacagcagta tctggttggg atttacaggt tttatcaggt aatcggaaat gttcgagccg
      901 atggcatctt ccatggtgtg ctcctgctcg ttgcgggtaa tcatcaccac gggcaggttg
      961 ggataatcgt tgcggatttg cgccagtgtt tccagtccgg tgaggccggg catgttttca
     1021 tccagaaaaa taatgtcgaa gtcgccgtcg cgaatctgat ccagggcctc atcaccgttg
     1081 ttggaagtgt gcacagcata gcctttttcc tgcaaaaaca aaatatgcgg ttttaataaa
     1141 tctatttcgt catctaccca taaaatttta actttggtca taatctaaca gtgttttgtg
     1201 gtgtaagaaa aaaaattgta atattgacac ggattattat ttacaaaaat acgaattaaa
     1261 aagataaaaa aaattggttc acaacaaaag aaagattata aacgatccgg ttcatggttt
     1321 tattactatc cccagcgaac tggtgtttga tattattgaa aatccctatt ttcagcggct
     1381 gcgtcatatc aagcaacttg gacttactta cctggtttac cccggagcta tccatacccg
     1441 ttttcaacat gcggtgggag cgcttcacct gatgacactg gctgtggaga tgctaaaggt
     1501 aaaaggaacc gagattaccg atgaggaaat ccagggactt tacctggcca tactgctgca
     1561 cgatattggc cacgggcctt tttcacatgc cctggaacac actttggtag aagaggtgag
     1621 tcacgaaacg ctgtcgctgc tttttttccg caggctgaac gaaacgtttg gcgggcggct
     1681 ggatatggcg atacggattt tttcggacca gtacccaaaa agatttctgc accagctggt
     1741 ttcgggccag ctggatgtgg accgcctcga ctatctgatg cgcgacagtt tttttaccgg
     1801 cgtgtcggaa ggtgtcatca gcacggaccg catcatcaaa atgatgaatg tttataacga
     1861 cgaattagtg gttgatgtca aaggagtgta ttccatcgaa aactttattg tggcccgccg
     1921 cctgatgtac tggcaggtgt acctgcacaa gacggtgctg tcggctgaac acctgttgat
     1981 acatattttg aaacgtgcca aattcctttg cgagcaaggc aaaaccattt ttgctccgcc
     2041 ggctttacag gaatttttaa aaaaccgttt tacgatagcc gattttaatg aaaaccccgg
     2101 cctgctcgat agttttgccc aactggacga ctatgatgtc tttatgtcgg tgaaaatgtg
     2161 gacacgcagc gatgacaggg tgctgtcgct gctcagcaaa aagctggtgg agcgcaaact
     2221 gctcaggata gaaatcagcg acacgccttt tgatgaaggg tatatcgata gcctgaagca
     2281 aaaggtgcag caggcctttg atatcagcgc cgaagaggct gactattttg tgttttcgga
     2341 agaagtggcc aataatgcct acaaccctta ttttgacaag atccatgtgt tatacaaaga
     2401 caaccgggtg ctggatataa ccgaaagctc cgaccagtta aacattaatc ttctgacaaa
     2461 aacggtccgt aaatactttt tatgctatcc caaaggcctg taataattaa accgaaagga
     2521 aaagcctgat tcgattgcgt atattggtgc gtcgataggc tcttcggggc tgtgagaggc
     2581 tctccggtgg gtttagcaac tcacagacgg tcgattgtgg gtagttattt atcccaaatc
     2641 tgaagacttc gaaaaagtag aataaagcat ctgactttta aagtgatttt ttttggattt
     2701 gggtgttcat ttcttttgct tgtcccccga caaaagtcgg ggcaggcgcc agaaatgaac
     2761 caaacctgcc tgacctgacg gcaacggcag gcagggaaaa agacaaccca gacaatgctt
     2821 cagcccgctt tcagaaaata ttgaaaatct aatcaaagta gtcccgacgg cgcgcgtcgg
     2881 gaaaccccgc aatctgcggg gcctttgaag attttcaaat attttctg
//
LOCUS       DAOSFP010000306         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32224_length_2927_cov_4.134053, whole genome shotgun sequence.
ACCESSION   DAOSFP010000306 DAOSFP010000000
VERSION     DAOSFP010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32224_length_2927_cov_4.134053"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLI16_08605"
     CDS             <1..722
                     /locus_tag="PLI16_08605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOH84657.1"
                     /translation="MGLGVHFINDMIGAQMRQGLMASYAYHLKLSEKWRLGLGLGLGL
                     SYNTLNGSKLIADEADDPAIPLTKESKLSFDAQTGIFAHNERFYAGFSVTNLLAGMSK
                     SENIITPNVSRHYYLTAGYLFDIGTKFKLKPSFLISEDFKSPTNIDISLYGIIIDKIW
                     LGANYRVNTTVGKKNLDNSLYHQNAVAFDIGWYVIDRLRIGYAYTLTLTTLKNYSGHE
                     IAIGYYFPSKVSPQMKSPRYF"
     gene            742..2727
                     /locus_tag="PLI16_08610"
     CDS             742..2727
                     /locus_tag="PLI16_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH84658.1"
                     /translation="MKTNIKNVIKLLVLSAALVFFSNSIFAQKQLQLAKDLMAKSEYS
                     EAIKVYKDVVKTPSAGIQEKRDLAACYLMTGNTLAAEEWLADVADNPDHTAQDLLHYA
                     DVLKSNGKYGEAIKQYEEYGKMLPAESSKVKEWVNACRKSQEWIADPAYTDVINADAI
                     NSENSDFGLIPYENGYVLTSDRRLKGKSYGASDIYGWTGNPYLKIIQYKDKDLGTTPR
                     ELENVNNNYHNGPAVYDKNSGTLYFTRTKMVKMIKKPMNCDPTSWYDNSKAKDYVNRL
                     ELYYATKEDGKYQQPVSFQYSNPQSHSVGHPALSPNGKIMYFVSDMAGGMGGTDIYYC
                     DKFTDGTWGTPKNAGKNINTTGNEVFPYVASDGTLYFSSDGWPGMGGLDLFSAKGSKD
                     SWATPENMKYPFNSPKDDFCMVYTGEGTGYLSSNRDGGKGQDDIYKFIPSPPKTFIFT
                     VTAKEKTDSVNLGMLKNAMVQVVGDSGECPVLYEKNGVYYFQADCGKEYKVKGSRDGY
                     FTAEKVITTNCLTRNDTVPVELILDKIVINKPIVLNNIYYDYDKWDIRPDAAFELDKL
                     VKILMENPKISIELGSHTDSRGKEKYNLDLSQKRAESAVNYIISKGIAQTRIKAKGYG
                     ETVPVNKCIDGVTCTEEEFQMNRRTEFKVTSISGGQK"
BASE COUNT          933 a          598 c          635 g          761 t
ORIGIN      
        1 atatgggact tggtgtacat tttattaatg acatgatagg tgcacagatg cggcaggggc
       61 ttatggccag ttatgcttac cacttaaaac tcagcgaaaa atggcgtttg ggattaggac
      121 tgggattagg cctgtcgtac aatacactca acgggtcgaa actgatagcc gacgaggccg
      181 acgatccggc tataccgctg accaaggaaa gcaaactttc ctttgatgcc cagacaggta
      241 tttttgctca caacgagaga ttctacgccg gattttctgt taccaatctt ttggccggca
      301 tgtcgaaaag cgaaaacatc attactccca atgtgagccg tcactattat cttacagcag
      361 gatacctttt tgatatcggt acaaaattca aattaaaacc cagtttcctt atttccgaag
      421 attttaaatc acctaccaac attgatattt ctctttatgg aattattatt gataaaattt
      481 ggctgggtgc caactaccgg gtgaacacta cggtgggcaa gaaaaatctg gataattcgc
      541 tttatcacca aaacgctgtg gcctttgata ttggatggta tgtaatcgac cgcctgcgga
      601 tagggtatgc ctatacgcta accctgacca ctcttaaaaa ttattcaggt catgaaattg
      661 caataggata ttatttcccg tcaaaggtta gtccgcagat gaaatcaccg aggtattttt
      721 aacttttaaa tttttgaaac tatgaaaaca aacataaaaa acgtgataaa gttattagtc
      781 ctttcggcag cattagtgtt tttttccaac agcatttttg cacaaaagca actacaatta
      841 gccaaagacc ttatggcaaa aagtgaatat agcgaagcca taaaagtata taaggacgtt
      901 gttaaaaccc cgtcagccgg tattcaggaa aaaagagacc tggctgcttg ttacctgatg
      961 acaggcaata cccttgcggc agaggaatgg ctggcagacg tggcagacaa tcccgatcat
     1021 acggcacagg acctgctgca ctatgctgat gtgttgaaat caaacgggaa atatggcgaa
     1081 gccatcaaac aatacgaaga gtatggcaaa atgctacccg cagaaagttc caaagttaag
     1141 gaatgggtta atgcgtgcag aaaatcgcag gaatggattg ccgatcccgc ctataccgat
     1201 gtgattaatg cagatgcgat aaactcagaa aacagtgatt ttggtttaat accttatgaa
     1261 aatggctacg tgcttacttc tgacagaagg ctcaaaggca aaagctatgg cgcatcggat
     1321 atctatggat ggacaggaaa tccttatctg aaaattatcc agtataaaga taaagacctt
     1381 ggcacaaccc ccagggaatt ggaaaatgtc aacaataact accacaatgg acccgctgtg
     1441 tacgataaaa actcgggaac actttatttt acgcgcacca agatggtcaa aatgattaag
     1501 aaacccatga attgcgaccc caccagctgg tacgacaatt ctaaagctaa agattatgta
     1561 aaccgtttgg agctttacta tgctaccaaa gaagacggaa aataccagca acccgtatca
     1621 tttcaataca gcaacccgca atctcattcg gtaggccatc ctgccttgtc gcccaatggc
     1681 aaaataatgt attttgtctc ggacatggcc ggcggcatgg gcggaaccga tatttattac
     1741 tgtgataaat ttaccgacgg cacctgggga acaccaaaaa atgccggaaa aaatatcaac
     1801 acaaccggta acgaagtatt tccttatgtg gcttctgacg gaacactcta cttttcaagc
     1861 gacggatggc ccggcatggg cggactcgat ctgttttcgg ccaagggaag taaggactcg
     1921 tgggctacac ccgaaaacat gaaatatcct ttcaattcgc ccaaagatga tttctgcatg
     1981 gtatatacgg gggaaggcac gggatacctt tcttcgaacc gcgatggagg gaaaggtcag
     2041 gatgatatct ataagtttat cccttctccg cctaaaacat ttatttttac ggtcacagcc
     2101 aaagaaaaaa ccgatagcgt taacctgggc atgctcaaaa atgctatggt acaggtagtg
     2161 ggcgatagcg gcgaatgtcc cgtgttgtat gaaaagaacg gcgtatatta ttttcaggct
     2221 gattgcggta aagaatataa agtaaaaggc agccgtgatg gctattttac cgcagaaaaa
     2281 gttattacca ccaattgcct gactcgtaat gatacagtgc ccgtggaact gatactcgac
     2341 aaaattgtca tcaacaaacc gattgttttg aataatattt attatgatta cgataagtgg
     2401 gatattcgcc ccgacgcagc gtttgagctt gacaagctgg tgaaaatcct gatggaaaat
     2461 cccaagatta gcattgagct cggttcgcat accgactcca gaggaaagga aaagtacaac
     2521 cttgacttat cgcaaaaacg cgccgaatct gctgtaaact atattatttc aaaaggcatc
     2581 gctcagacca ggattaaagc aaaaggctat ggcgaaaccg taccggttaa caaatgtatt
     2641 gatggcgtaa cctgcaccga agaagaattt cagatgaaca ggcgtaccga atttaaagtt
     2701 accagtatca gtggaggaca gaaataaaaa tggctgttta agttgagcag catctattga
     2761 aaaaactaat caggaagccg gatttttccg gcttcttttt tttgtggtgt tccggatacg
     2821 cttattaaca ttgaacctgc acatgacgta ttaacagttt tatgttgata ttatttttag
     2881 caatacctta gtaagttggg tattaatagc aacctttgca atattat
//
LOCUS       DAOSFP010000307         2919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32336_length_2919_cov_5.940992, whole genome shotgun sequence.
ACCESSION   DAOSFP010000307 DAOSFP010000000
VERSION     DAOSFP010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32336_length_2919_cov_5.940992"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLI16_08615"
     CDS             complement(<1..1005)
                     /locus_tag="PLI16_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOH84659.1"
                     /translation="MSFSKFSVFLWLALALFFFCGCGNDNKPVDEFVIHGKLKNSLGE
                     KIVLQQLGLDSIKPVDSLQVDENGEFTMTYKPQDISFYLLKVSEENFIMLLADKGETI
                     EITGNIRQLANEYAVTGSAGSALLWELNSYTRKNINKTDSLYKIQLRQQDSANFPQVK
                     QHLDSVYNTIFEDQRRYVQGFITKNSTSLASIIAIYQVFGRQKVLNERDHFSTFKMLD
                     STLFLLYPNNEYVLELHKRVSDVKKAQADKLKNLAKLDSAMVAPEIKMKNIGGFLQPL
                     SSLKGHVTLVLFWAASSRPSIAALEQYKWIHKKYREKGFQVYAVSLDKNRRDWEFAVN
                     E"
     gene            complement(1014..1631)
                     /locus_tag="PLI16_08620"
     CDS             complement(1014..1631)
                     /locus_tag="PLI16_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84660.1"
                     /translation="MDQENKTPEPATPAAEQPTKKGLSAQTIVIIIILALMIIIPLVI
                     FLWKQAEIKSLKQSHETRISEIITQANNTIMENNRKNIETVTRVFTWAVRSEMLRQNM
                     EQINTYMTDLVKMEGMHHISVINADGIVVSSTNKKYEGVVYPGPIAKELSGITEAITK
                     SDESGDMVSICPVMGLDNRMGTLIITYQPKTNIFVPKEAVSEEKK"
     gene            1831..1901
                     /locus_tag="PLI16_08625"
     tRNA            1831..1901
                     /locus_tag="PLI16_08625"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1863..1865,aa:Cys,seq:gca)
     gene            2092..2577
                     /locus_tag="PLI16_08630"
     CDS             2092..2577
                     /locus_tag="PLI16_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84661.1"
                     /translation="MKKLIISLFTVLMLLCTNYAYPQDSCRVLKHEISAKYQGHCKKG
                     LANGKGFAEGIDKFEGRFKNGFPNGAGIYTWANGDTYNGIFKDGLRNGEGTFKYKKNG
                     ADSVLHGIWKNDVFVKKILPTPYKIYIARDFDRYSITKLREGNKVSLKLHQMGYSGSL
                     S"
     gene            complement(2706..2915)
                     /locus_tag="PLI16_08635"
     CDS             complement(2706..2915)
                     /locus_tag="PLI16_08635"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HOH84662.1"
                     /translation="MDGKGRATDNIYIERLWRNVKQENIYLNAYETGLGLYKGLGEYF
                     YQYNNHRVHQALNYLKPIDIYKNVA"
BASE COUNT          780 a          573 c          638 g          928 t
ORIGIN      
        1 ctcattaacg gcgaattccc agtcgcggcg gtttttgtcc agcgaaacgg catatacctg
       61 gaatcctttt tcgcggtatt ttttgtgtat ccacttatac tgttcgagtg ctgctatgct
      121 cggccggctg gatgctgccc agaagagcac cagagtaaca tgtcctttga gcgatgagag
      181 gggttgaaga aaaccaccta tatttttcat ttttatttcg ggggccacca tggcggaatc
      241 tagtttcgcc aggtttttga gcttgtcggc ctgtgccttt tttacatcgc tgacccgctt
      301 atgcagttcg agcacgtatt cgttgttggg atacaataga aacagcgtgc tatcgagcat
      361 tttaaacgtg ctgaaatggt cgcgttcgtt gagcactttt tggcggccaa agacctgata
      421 aatagcgatg atcgacgcca gcgatgtgct gttttttgtg ataaaaccct gcacatagcg
      481 gcgctggtct tcaaaaatag tgttatatac agaatccaga tgttgtttta cctggggaaa
      541 gttggcgcta tcctgttgac ggagctggat tttataaagg gaatcggtct tattgatatt
      601 tttccgggta tagctgttca gctcccagag caaggccgag ccggcagagc ctgtaacggc
      661 gtattcattg gccagttgcc tgatgttgcc tgtaatttct attgtctcgc ctttgtcagc
      721 caacagcata ataaagtttt cttcagaaac tttcagcaaa taaaaactga tgtcctgggg
      781 cttatatgtc atggtaaact cgccattttc gtccacctgc agcgaatcaa ccggcttgat
      841 actgtcgagg ccgagttgtt gcaacacaat cttttcgcca agggaatttt ttagctttcc
      901 gtgaatgaca aactcatcca caggcttatt gtcgttgccg caaccgcaga aaaaaaacag
      961 ggcaagggca agccacaaaa aaacggaaaa cttactgaaa gacatcgcgc tatttatttc
     1021 ttttcttccg acactgcttc tttggggaca aagatattgg ttttgggctg ataagtaatg
     1081 atcagggttc ccattctgtt atcgagaccc atcaccgggc agatagaaac catatcgccg
     1141 ctttcgtccg atttggtaat ggcctcggtg atgcccgaca gttctttagc aatagggccg
     1201 gggtagacta caccttcata ttttttgttg gttgagctga ctacaattcc gtcggcattg
     1261 attacggaga tatggtgcat accttccatt ttcaccaggt cggtcatata ggtattgatt
     1321 tgctccatat tttgtctcag catttcggaa cgcacagccc aggtaaaaac acgggttacc
     1381 gtctcgatgt ttttgcggtt gttttccata atggtgttgt tggcctgtgt gatgatttcg
     1441 ctgatacgtg tctcatggct ttgtttcagg ctttttatct cggcctgctt ccacaggaat
     1501 atcaccagcg ggatgataat catcagggcg aggataatga tgattacgat ggtttgggca
     1561 cttaaaccct tttttgtggg ttgttccgct gcgggggttg cgggttcggg ggttttattt
     1621 tcctggtcca ttttttaaaa ttttaataca aagctacgtt atttatgcta ataaaagata
     1681 gtttttatca gaaagaacag ccgttgtatt ttttttacga tgatgaaaat atcttaaaaa
     1741 ataaggcatt tcggcaaggg ctatgtgcaa tgatgccgga aaaaattttt ttgcttacaa
     1801 aaatatttta tatctttgcc ctcctaacaa ggtactatgg ccgagtggtt aggcagaggt
     1861 ctgcaaaacc tcgtacagcg gttcgaatcc gcttggtacc tcaaaaacat tctgaaaatc
     1921 agttatttaa ataactgaaa aagacaataa aagaagcagt gaaaactgct ttttttgttt
     1981 tattccggca taaaaaatcc tcttgattta taaggctttt ttgtttaaat ttgtgttaga
     2041 cttgttaatc cttttaaggc aaagcacaat aaaaaaataa acctgcaggc tatgaaaaaa
     2101 ctgataatat ccttgtttac cgtactcatg ctgctttgca caaattatgc ttatccgcag
     2161 gatagttgcc gggtgttaaa acatgagatt tccgcaaaat accaggggca ttgtaaaaag
     2221 ggactggcaa acgggaaagg ctttgccgaa ggtattgata aatttgaagg ccggtttaaa
     2281 aacggatttc ctaacggagc aggcatatac acatgggcta atggtgacac ctataatggc
     2341 atttttaaag atggcctgag aaacggggaa ggtactttta agtataaaaa aaatggtgcc
     2401 gacagcgtct tgcacggcat ctggaaaaac gatgttttcg tcaaaaagat tttgcctacc
     2461 ccctataaga tttacatagc gcgcgacttt gacagatatt ctattacaaa actccgggaa
     2521 ggcaataaag tttcgcttaa attacatcaa atgggctata gtggttccct gtcttaggac
     2581 agatttttta taattcttaa ttgatttttc ttttcagaga aacagatgac gaagcacctg
     2641 tttcccgttg aaaagatatt tttttccctt tgcaaatata aaaattattt gttcgaattt
     2701 ttcttttaag ccacattttt ataaatatct atcggtttta aataatttag tgcttgatgt
     2761 acacgatgat tgttgtactg gtagaaatat tcccctaatc ctttgtacag ccctaatcct
     2821 gtttcgtagg cattaaggta gatattttct tgcttcacgt ttcgccataa gcgttcaatg
     2881 taaatgttgt ctgttgcacg gcctttacca tccatgctt
//
LOCUS       DAOSFP010000308         2898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32685_length_2898_cov_3.863173, whole genome shotgun sequence.
ACCESSION   DAOSFP010000308 DAOSFP010000000
VERSION     DAOSFP010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32685_length_2898_cov_3.863173"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1573
                     /locus_tag="PLI16_08640"
     CDS             113..1573
                     /locus_tag="PLI16_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOH84663.1"
                     /translation="MTEKIINEKYLASAINRAKEKGVILPTFRQMENPELIPEKIKNK
                     LKDIGLWDLHPLNLFRITWKNEPKKTGGLYDGVNYLELPKEITGVDARIVFLVGKWFP
                     TGAHKVGAAYGCLVPRIVTGQFDPAFHKAVWPSTGNYCRGGAFDSYLLGVTAVAILPE
                     EMSKERFNWLKEIGAEVIATPGCESNVKEIYDACWDIRKNRKDCVIFNQFEEFGNPCW
                     HYHVTGKAVEEVVKSLGGNLSAYVSATGSAGTIGAGDYLRTVYPHIKVVASEALQCPT
                     LLMNGFGGHRIEGIGDKHVPWVHNVKNTDMVTAIDDEDCMRLLRLFNEPEGKKHLADA
                     GLSSEIISQLSLIGISGIGNLLSAIKAAKHYEMTSEDIIVTLATDSAEMYQSRVTELA
                     EQKGAYNSLQAAKDFEKCLFGTGTDNIKELTYTDRKAIHNLKYYTWVEQQGKTYEEIN
                     QLWYDRNFWPDTFNQVTYWDEKIDEFNERSGVLKNL"
     gene            1576..2751
                     /locus_tag="PLI16_08645"
     CDS             1576..2751
                     /locus_tag="PLI16_08645"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOH84664.1"
                     /translation="MCEFLKNPFYREVPQWTNQENAFMNEDILNFHRLLEGYEPTPVH
                     SLNGLAKKLGIGSVYVKDESFRFGVKAFKPLGASYTIFRFLKSKWEDSYASEFTVSDF
                     KNHEKMAALGAFTFCAATDGNHGRAVAWTARILKQNAVIYMPANTVASRIKNIESEGA
                     RVVLIDGTFDECVSRADADAKANGWIIISDTAYPGYMFYPQFIMAGYSTIFREMDGIV
                     TMPSSCKADIVILQAGVGGLAAAGTWYMTHKYGKNRPRIICLEPTRADAFMESVKNGK
                     PTHSKQDYNSIMAGLNCGVSLLAWDYISQGANYMLTVSDTYAEKAMRQYYYPEGNDPQ
                     IISGESGASGMAGLLALCEEEKFSTLRNELGMNSKTSVLLINSEGDTDPVNFKKVIS"
BASE COUNT          871 a          673 c          654 g          700 t
ORIGIN      
        1 cataggtatt tttaaatatg tgcaaatttt actcctccaa tgactaattt ttttactttt
       61 gaacccgcaa atttttcata ctacaaaaac catactaaaa aataaattat ccatgactga
      121 aaaaattatt aacgagaaat accttgcaag tgccataaac agggcaaaag aaaaaggcgt
      181 tatcctgccc actttccggc agatggaaaa ccctgaactg ataccggaaa agataaaaaa
      241 caaattaaag gatataggcc tatgggacct ccaccccctg aaccttttcc gcatcacatg
      301 gaaaaacgag ccaaagaaga cggggggatt atatgatggt gtaaactatt tggaacttcc
      361 caaagaaata accggggtgg atgcccgcat agtttttctg gtcggaaaat ggttccctac
      421 cggcgcccat aaagttggcg cagcatacgg ctgtcttgtc ccccgtatcg ttaccggtca
      481 gtttgatccc gcgtttcata aagccgtatg gccttccacc ggtaattatt gccgcggcgg
      541 cgcctttgac tcatacttgt taggcgttac cgcagtggcc atcctgcccg aagaaatgag
      601 taaagaacgt ttcaactggc tgaaagaaat cggtgccgaa gtgatagcta caccgggttg
      661 cgaatcaaac gtaaaagaaa tttacgatgc ctgttgggat atacgcaaaa accgcaaaga
      721 ttgcgttatt ttcaaccaat tcgaagagtt tggaaatccc tgctggcact accatgttac
      781 gggtaaagcc gttgaagaag tagtgaaatc cctcggaggg aatctctccg cttatgtcag
      841 cgctacgggt tcagcaggaa ccattggcgc cggagactac ctgcgaactg tttatcctca
      901 tatcaaagta gtagcttccg aagcattgca gtgtcccacc ctgctcatga acggctttgg
      961 cggacaccgc atcgaaggca ttggcgacaa acatgttccc tgggtgcaca acgtcaaaaa
     1021 caccgacatg gttacagcca ttgatgatga agactgcatg aggctgttgc gcctgtttaa
     1081 tgaacccgaa gggaaaaaac accttgcaga cgccggctta tcttcagaaa ttatcagcca
     1141 gttatcgctg attggtattt caggaattgg caatctgtta tcggccataa aagctgcaaa
     1201 gcactatgaa atgacctccg aagatatcat cgtaacactg gccacggatt ctgctgaaat
     1261 gtaccagtct cgtgttacag aacttgctga acaaaagggc gcctataaca gcctgcaggc
     1321 tgccaaagac tttgaaaaat gcctgttcgg aacaggtacc gacaacataa aggaactgac
     1381 atataccgac agaaaagcca tccacaacct gaagtattac acatgggtgg aacaacaagg
     1441 aaaaacctat gaagaaataa atcaattgtg gtatgaccgt aatttctggc ccgacacctt
     1501 taatcaggtg acctattggg atgaaaaaat cgatgaattc aacgaacgca gcggcgtatt
     1561 gaaaaactta taaccatgtg cgagtttctc aaaaatccgt tttataggga agtcccccaa
     1621 tggacaaatc aggaaaatgc tttcatgaat gaggacatcc tgaattttca ccgtttgctg
     1681 gaaggttatg agcccacgcc ggtgcactcc ctgaacggcc tggctaaaaa attggggata
     1741 ggttcagtgt atgtgaaaga tgaatcgttt cgctttggtg tcaaggcatt caaaccgctg
     1801 ggagcttctt ataccatttt ccgttttctg aaatcaaaat gggaagatag ctatgcgtcg
     1861 gagttcacgg tgtcggactt taagaatcat gaaaagatgg ccgccttagg agcctttact
     1921 ttttgtgccg ccaccgacgg caaccatggg cgcgccgtgg catggaccgc ccgcatcctg
     1981 aaacagaacg ccgtgattta tatgcccgca aacacggtcg cttcccggat aaaaaacatt
     2041 gaaagcgagg gtgcccgtgt agtacttatc gacggcacct ttgatgaatg tgtaagccgc
     2101 gcagatgccg atgccaaagc caacgggtgg atcatcattt ccgatacagc ctatcccgga
     2161 tatatgtttt atccccaatt tatcatggcc ggatattcca ctatcttccg cgaaatggat
     2221 ggtatcgtta ccatgccgtc gtcctgcaaa gcagacatcg tcatactgca ggccggtgtt
     2281 ggcggactgg ccgctgctgg cacctggtat atgacacaca agtatggcaa aaaccggcca
     2341 cggataattt gcctggaacc tacccgtgcc gatgcattta tggaatccgt taaaaacggt
     2401 aaaccaactc attcaaagca ggattataat tctatcatgg ccggcctcaa ctgcggggtt
     2461 tcattactgg catgggatta tatcagccag ggtgcaaatt atatgcttac cgtcagcgac
     2521 acctatgccg aaaaagctat gcggcaatat tattaccccg aaggcaacga cccgcagatt
     2581 atttcgggtg agtcaggcgc ctcaggtatg gcagggctgc tggcgctttg tgaagaagaa
     2641 aagttttcaa cactccgaaa cgaactgggc atgaactcca aaacctctgt attgctcatc
     2701 aattcagaag gagataccga tccggtaaat ttcaaaaaag taatatcata gaaaacctca
     2761 acctggaata taaaaaagta tttttatgat tgtattcaaa ctcaacgggg aaaatatcaa
     2821 ttatgacggc gatcccaaaa aacctctgct gaaatacctt cggaacgata aacacatcac
     2881 ttcctgcaaa gatggctg
//
LOCUS       DAOSFP010000309         2884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32930_length_2884_cov_3.694592, whole genome shotgun sequence.
ACCESSION   DAOSFP010000309 DAOSFP010000000
VERSION     DAOSFP010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32930_length_2884_cov_3.694592"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="PLI16_08650"
     CDS             <1..265
                     /locus_tag="PLI16_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84665.1"
                     /translation="SSSSHPSDWFFVIWLFMMGLSAFLVRLFIDTGILENQFWMYLFH
                     FTVLVQWALIIVPFGKWTHFLYRSFAMYFASIINKTLSVKTKK"
     gene            549..944
                     /locus_tag="PLI16_08655"
     CDS             549..944
                     /locus_tag="PLI16_08655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOH84666.1"
                     /translation="MEIKVTFDGNKKVNAIVRNHIVKTDQPINGGGEDTAVSPYELFL
                     ASLATCAGIYVKGFCDNRNIPAENITLTQNHEFNEKGLLKKVELTINVPKDFPEKYVD
                     SLIHVASLCKVKQQLMSPPELSVVSKIIE"
     gene            1138..1449
                     /locus_tag="PLI16_08660"
     CDS             1138..1449
                     /locus_tag="PLI16_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOH84667.1"
                     /translation="MLTFLKNLFSPQPKADFGALIKQGATIVDVRSKGEYQGGHLKGS
                     INIPLKNLADNLAKIKKDKPVITCCASGARSGVAKSMLKANGYDVFNGGPWESLKKHC
                     N"
     gene            complement(1455..2267)
                     /locus_tag="PLI16_08665"
     CDS             complement(1455..2267)
                     /locus_tag="PLI16_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84668.1"
                     /translation="MKKTFFIPLVFLTFMAIFSACKKEDVLPEDKNTGKIALKFTHKV
                     DGQAIEMDTLKYINAAGNHYMVNEIQYFISDVTLHNADGSSFMIKAWEDIHYVDTDLP
                     STWTWNVLDSIPAGTYNSISFTFGISQAKNQSFMFVNPPENNMFWPEFLGGGYHYMKL
                     NGKWSDGVSAALPYEFHLGIGQIYSGTGTSIDSIIGFVQNYFTVDLPNSSFTLAKNET
                     RTIDLVMNVESWFETPHVWDFNVQGTYIMQNQAAMQLAKENGFDVFSVSSIQ"
     gene            complement(2283..2648)
                     /locus_tag="PLI16_08670"
     CDS             complement(2283..2648)
                     /locus_tag="PLI16_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84669.1"
                     /translation="MKNILILLSILTLTIFSACEKKNAHKVTYYVTNSQQGFTASYLD
                     ETGALQTEYIPTQSASEKKIIKTYWANAGDIVYISVRDTATYSFVKATIFVDDKVYKE
                     ASRTDDKTKPLTVSGTIPY"
     gene            complement(2661..>2884)
                     /locus_tag="PLI16_08675"
     CDS             complement(2661..>2884)
                     /locus_tag="PLI16_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84670.1"
                     /translation="HVSEAFYEPTTPGFALFNLGLSYKYNEHISISGGVNNLFDKAYY
                     EHLNRKMLGLPTKLYEPGRVFYINLFFNI"
BASE COUNT          888 a          562 c          588 g          846 t
ORIGIN      
        1 aagttcatct tcgcatcctt cggactggtt tttcgttatc tggctgttta tgatgggact
       61 cagcgctttt ctggtgcgtt tatttattga taccggtatc ctcgaaaacc aattctggat
      121 gtatcttttt cattttaccg tgctggttca gtgggcacta ataatcgttc catttggcaa
      181 atggacacat tttctgtacc gttcttttgc catgtatttt gcaagtatta ttaacaaaac
      241 tctatcagtt aaaacaaaaa aatagttcta ttcatttttt taacaaaaac attgccttcg
      301 tgcctttgtg gcgaacgaga aagaaagcaa tgccactaag acactaagac acaaagaaaa
      361 aatgaaataa aagtgaatta aaaaacttag tgccttcgtg cctttgtggc gaataagaaa
      421 gaaagcaatg ccactaagac actaagacac aaagaaaaaa gatgtaatca gaaagactta
      481 ataaacatag tgccttcgtg cctttgtggc taaaaaccaa aaaataataa atttaaataa
      541 ttataaaaat ggaaattaaa gtaacattcg acggaaacaa aaaagtaaac gccatcgtgc
      601 gtaatcacat cgtaaaaacg gaccaaccta taaatggtgg tggcgaggac actgccgtgt
      661 cgccctatga attgtttttg gcttcgttgg ctacctgtgc cggtatttat gtaaaaggat
      721 tttgcgataa caggaatatt cctgccgaaa atatcactct gacacaaaac cacgaattca
      781 acgaaaaagg tttgctgaaa aaagtggaac tgactatcaa tgtacccaaa gacttcccgg
      841 aaaaatacgt ggattcacta attcatgtag caagtttatg caaagtgaaa caacaattga
      901 tgtcacctcc cgaactttca gtcgtttcaa aaatcatcga ataaaaataa caggcttaat
      961 tggctaataa tttattagtt gcaagtagaa agtagaaagt ggaaagtgga aagtagaaag
     1021 ttagttgtat tgagcaattt gcgactagca acttgcgact tgtaacttgt accttgcgac
     1081 ttttattaag ttaacgccca aagttcccta aacctctaat cccctaaaca tccatatatg
     1141 ctaacattcc ttaaaaatct cttttccccg caacccaagg ccgatttcgg tgcattaatt
     1201 aaacagggcg cgaccattgt tgacgtcagg tcaaaaggag agtaccaggg cggacattta
     1261 aaaggttcca taaacatacc tttgaaaaat ctggcagaca acctcgctaa aataaagaaa
     1321 gataaaccgg tgattacctg ctgtgcttcg ggtgcccgta gcggagtcgc gaaaagtatg
     1381 ttgaaagcca atggttatga cgtttttaac ggcggccctt gggagagcct gaaaaaacat
     1441 tgtaactgat tggtttattg tatcgaactg acactgaata catcaaaacc attttctttg
     1501 gctaactgca tagctgcctg gttttgcatg atataagtgc cttgtacgtt aaaatcccat
     1561 acatggggag tttcgaacca gctctccacg ttcatcacca gatcaatggt gcgggtttcg
     1621 tttttggcaa gggtaaatga tgagttgggc aagtcaacag tgaaatagtt ttgtacaaaa
     1681 cctattatgg aatcaatgct tgtccctgtt cccgaataaa tctgtcctat gccaaggtgg
     1741 aactcatagg gcagagctgc tgatacgccg tcactccact tgccgttgag tttcatatag
     1801 tgatagccac cacccagaaa ttcgggccag aacatattgt tttccggtgg gtttacaaac
     1861 ataaaagact gattcttggc ttgcgaaatg ccaaaagtaa acgaaatcga gttgtaggtc
     1921 cctgccggaa tcgaatccag aacattccag gtccatgtcg aaggcaggtc ggtgtcaaca
     1981 taatggatgt cttcccaggc ttttatcata aagctgctac catctgcatt gtgcagggtg
     2041 acgtcagaaa taaaatactg aatttcgttc accatgtaat ggttgccggc ggcattaatg
     2101 tattttaaag tatccatctc aatagcctgt ccgtcaactt tatgggtaaa ttttaacgca
     2161 atttttccgg tatttttatc ttccggcagt acatcttctt ttttacaggc cgaaaatatt
     2221 gccataaaag tcaaaaaaac cagagggata aaaaatgttt ttttcatgcg tttcatgtga
     2281 ttttaataag gtattgttcc tgatactgtt aacggctttg ttttgtcgtc agtgcgggaa
     2341 gcttctttgt aaactttgtc atccacaaat atagtcgctt tgacaaacga ataggtcgcc
     2401 gtgtccctga ccgaaatata aacaatatcg ccggcattcg cccaataggt tttgatgatt
     2461 ttcttttcgc tggcactctg ggtaggaatg tattcggtct gcagggctcc ggtttcgtca
     2521 agataggaag cggtgaatcc ctgttgtgag ttggttacat aataggtgac cttgtgagcg
     2581 tttttctttt cacaagccga aaaaattgtc agtgtaagga tgctaagtag gattaaaata
     2641 tttttcatgg gtaacaggtg ttaaatgttg aaaaacaaat tgatataaaa aacccgtccg
     2701 ggctcataca acttggtagg caagcctaac atcttgcggt tcaggtgttc gtaatatgcc
     2761 ttatcgaaca ggttgtttac ccctccgcta atacttatgt gctcattgta tttatagctt
     2821 aatcccagat taaacagtgc gaatccgggt gtggttggct cgtaaaaagc ttccgacaca
     2881 tgtc
//
LOCUS       DAOSFP010000310         2884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32933_length_2884_cov_3.475786, whole genome shotgun sequence.
ACCESSION   DAOSFP010000310 DAOSFP010000000
VERSION     DAOSFP010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32933_length_2884_cov_3.475786"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLI16_08680"
     CDS             <1..902
                     /locus_tag="PLI16_08680"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOH84671.1"
                     /translation="KFLHQGNTVVISAMEHHSNIVPWQLACEERQAHLKVIPINEQGE
                     IIFDEVEKFLDKNCKILAITHVSNTLGTINEIKQLINLAHAHEIPVLIDGAQAISHIK
                     VDVQALDCDFYCFSGHKMYAPMGVGVLYGKEKILDEMPPYQGGGEMIKTVSFAKTTFN
                     ELPFKFEAGTPSVGDVLGLKEAIRFIENISYEVMEAHEHELLRYGTEKLEQLGDIRII
                     GTAPQKTSVISFLPGNIHPFDAGTILDKLGIAVRTGHHCTQPLMDFYKISGTVRASFA
                     IYNTKEEIDAMIAALQRIKIMFA"
     gene            902..1336
                     /locus_tag="PLI16_08685"
     CDS             902..1336
                     /locus_tag="PLI16_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOH84672.1"
                     /translation="MKTYQETAAEIISEFMLFTDWMDKYNYLIELGKALPLIDEQFKT
                     DNYLIAGCQSQVWVKAEYKDGAVFFTADSDAIITRGIVSLLIRALSGRPCDEIINADM
                     EFINQIGLREHLSPTRSNGLNSMIKQMKMYALAFKTKHESNL"
     gene            1340..1651
                     /locus_tag="PLI16_08690"
     CDS             1340..1651
                     /locus_tag="PLI16_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOH84673.1"
                     /translation="MDKAELEKIIIEKLQTIYDPEIPVNIYDLGFIYEIRIDDNYQVT
                     IKMTLTSPKCPVAETLPIDVRDGIQAISGIKSVYVDVVFEPEWNQDMMTDAAKLELGL
                     L"
     gene            complement(1818..2663)
                     /locus_tag="PLI16_08695"
     CDS             complement(1818..2663)
                     /locus_tag="PLI16_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84674.1"
                     /translation="MKKYFFLCICALPFLWSCNNTGENAGDEIDPKDQQIKELQAQLA
                     EKDANVNDMFSFINDVEDNLNTIEASQMKISESKKGNNEVEQDVKESIMEHITNINTL
                     LEKNKELIKKLEGALRNSNMKVDALNKTIELLNQQMTQKDAEIADLKVQLEKLNFTVA
                     ELSTKIEDLTGQNEEKAQLIDQQDVELNTAYYVIGVRKELKAKGVIVGDKISQKINPE
                     NFTKIDIRKTTEITLNVKKAKLLSTHPEGSYTMLMKGKTIDKLVINVPKTFWSISKFL
                     VIELD"
BASE COUNT          853 a          618 c          578 g          835 t
ORIGIN      
        1 aaaaattcct ccaccaggga aacacggtag taatatccgc catggaacac cattccaaca
       61 tcgtaccgtg gcaattggcg tgcgaagaac ggcaggccca cctgaaagtt atccccataa
      121 acgaacaagg tgaaattatt ttcgacgagg tggaaaaatt tctggacaaa aattgcaaaa
      181 tactggccat tacccatgtg tcaaataccc tgggcaccat caacgaaata aagcaactca
      241 taaatctggc gcacgcccat gaaattccgg tattgattga cggggcgcag gccatctcgc
      301 atatcaaagt ggatgtacaa gcgctggatt gcgattttta ctgtttttcg gggcacaaga
      361 tgtatgcccc tatgggcgta ggagtgcttt acggtaagga aaaaatactt gacgaaatgc
      421 ctccctacca gggcggcggc gaaatgataa aaacagtaag ctttgcaaaa actaccttta
      481 acgaactgcc tttcaagttt gaagccggca cacccagtgt gggtgatgtc ttaggcctga
      541 aagaagccat ccgcttcatt gaaaacataa gttatgaagt gatggaagcc cacgaacacg
      601 aattgctccg gtatggcaca gaaaaactgg aacaattggg tgacataaga ataattggta
      661 cagcaccaca aaagacatca gtgatttcgt ttcttccggg aaatattcat ccttttgatg
      721 ccggtaccat ccttgataaa ctgggtatcg ccgtgcgcac gggacaccac tgcactcagc
      781 cgcttatgga tttctacaaa atctccggca cggtaagggc ttcttttgca atttataata
      841 ccaaagaaga aattgatgcc atgatagcgg ctcttcaaag aataaaaata atgtttgcat
      901 aatgaaaacc tatcaggaaa cagcagcgga aatcatcagc gaattcatgc tttttaccga
      961 ctggatggac aaatacaatt acctcatcga gttgggcaaa gcattaccgc ttattgacga
     1021 acaattcaaa accgacaact accttattgc aggctgccaa tcgcaggtgt gggtaaaagc
     1081 cgaatataaa gacggcgcgg tttttttcac agccgacagc gatgccatca tcaccagggg
     1141 tatagtcagt cttcttattc gtgccttatc gggaaggccc tgcgacgaaa ttatcaacgc
     1201 cgatatggaa tttatcaacc agataggctt gcgcgagcac ctttccccta cccgctccaa
     1261 cggattgaat tccatgataa aacaaatgaa aatgtacgca cttgcattca aaacaaaaca
     1321 cgaatcaaac ctttaaatta tggataaggc cgagctggaa aaaatcatca tcgaaaaatt
     1381 acaaactatt tacgatcccg aaatcccggt aaatatttac gatctggggt ttatttatga
     1441 aatccgaatc gatgacaatt accaggtaac gataaaaatg acacttacct cgcccaaatg
     1501 cccggtagca gaaactttac ccattgatgt ccgcgatggc atccaggcta tttccgggat
     1561 caaaagtgtt tacgtagatg tggtctttga acccgaatgg aatcaggata tgatgacgga
     1621 cgccgctaaa ctggagctgg ggttgttata atctgagatt tataaaaaac tttatcatct
     1681 gcgtacttag cccagaaggc ttatgggctg aatcctttca aaatgaaaac aactaacagt
     1741 aagcacttca ttttgcagac gatgctttta ttaatttaca aagaaaaaat cttcaacaga
     1801 caggctgccc ggcacattta atccaactca atcacgagaa atttggaaat actccagaaa
     1861 gttttcggga catttatcac cagcttatca atggtttttc ccttcattaa catcgtatag
     1921 cttccttcgg gatgggttga cagcaatttg gctttcttta cattcagggt aatttcggtt
     1981 gttttgcgga tatcgatttt tgtaaaattt tcgggattga ttttctgaga aattttatca
     2041 ccaacgataa cacctttggc tttcaattct tttcttaccc caataacata ataggccgtg
     2101 ttaagctcca catcctgctg atcaatgagc tgggcttttt cctcgttttg tccggtaaga
     2161 tcttctattt tggtactaag ttcggccaca gtaaaattga gtttttccaa ttgtactttc
     2221 aggtcggcaa tttcagcatc tttttgtgtc atctgctggt tgagcagttc aatggtttta
     2281 ttcagggcat ccaccttcat gtttgaattg cgcagggcac cttccagttt cttaatcagt
     2341 tccttgtttt tttcaagcag ggtattgata tttgtgatat gctccatgat gctttctttt
     2401 acatcctgct ctacctcgtt atttcctttt ttcgactcgg aaattttcat ctggctggct
     2461 tcgatagtgt tgaggttatc ctccacatcg ttgatgaaac tgaacatgtc gttaacattg
     2521 gcatcttttt cggccagttg cgcctgcagt tcttttatct gctggtcttt cggatcgatc
     2581 tcatcaccgg cattttctcc ggtattattg caactccata aaaatggcag tgcacatata
     2641 cataaaaaga aatacttttt catgattttt cttttgttaa tagtttacaa tacaaagata
     2701 aacggaaaat ttcgccggtg attgtgtttt accatatttt tattgggaag cctatggtaa
     2761 ctatgtaaag atcctttata aggtaggaat tgggcataac cttatccgca aaaatccgga
     2821 cataaaaaag aggctgtctc aaaagggcag cctctttaat ttattgatta tcaacacgat
     2881 aatg
//
LOCUS       DAOSFP010000311         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_32991_length_2881_cov_3.959306, whole genome shotgun sequence.
ACCESSION   DAOSFP010000311 DAOSFP010000000
VERSION     DAOSFP010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32991_length_2881_cov_3.959306"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLI16_08700"
     CDS             complement(<1..617)
                     /locus_tag="PLI16_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84675.1"
                     /translation="MSNKSKLIWVYVISAIFIALNIYFIIKEFYWFSLIPIVLILLLL
                     FFFALDKVLYIITFCTPLAINLMDFDDRLSLSLPTEPLLFGVLIFFFIKLVFERQYDT
                     KVLKHPVTIAIIINLLWMFITALTSELPLVSIKFLISRLWFVVPFYFVAVILFKKLNN
                     IRIISWMYAIPLVIVIIYSTYRLYIWSFDEQAAHWVMDPFYNDHTA"
     gene            complement(626..1585)
                     /locus_tag="PLI16_08705"
     CDS             complement(626..1585)
                     /locus_tag="PLI16_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84676.1"
                     /translation="MNNFFKSTYLIKLILRWKWHLLIITLLAIVLSSVFSAPAFIKPK
                     YKSYAVIYPANISPYSSETNTEQMLQLLKSEDMLFDIVNKFDLNKEYKIDTVDKYYRS
                     KIISELEDNVEIKRTEYESVLIEVYDREPGQACAIVNEFINLMNSKARNLQRSKTAEV
                     VKLYGDQMEDKKKQIDSIEKRLQLLRNEYNILDYNIQVEEYSKGYVKNITAGRGNKTD
                     DIAVTLNNLKQYGGEFRQLDANLWGLIDSYIALKKEYDKSVSDLNKELTYANVVTQPV
                     AADKKSYPIRWLIVCISTVSAFLLSLLLFSIIEGAQKPEKISQ"
     gene            complement(1705..2754)
                     /locus_tag="PLI16_08710"
     CDS             complement(1705..2754)
                     /locus_tag="PLI16_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOH84677.1"
                     /translation="MAEQDIRQANFNTTNFFFFLYKWRKPLIIICLVAAIVSSVISLL
                     ITPKYKSSVILFPASTNAISKALLADNYGGKLDILEFGEEAQTEQLLQILSSNEIRSR
                     VIKKFNLMEHYEIENDSKYKMTRLFEEYNSNITYRRTEYMAVEITVLDKDPQLAADIA
                     NYISDQLDSVKNKMQKERAIKAFQIVETEYKKLQNDIKSLEDSLTVLRKMGVNDYETQ
                     AEAYNTQLAIALSKNNREAVRAIEERLQLLGDYGSAYVSLRDELELEKKQLSAIKAKY
                     EEAKVDATQDLPSKFVVDKAFKAERKSYPVRWLIVMLSVVSVFFISIMTLIIIENFSK
                     KKFPTNKPEDIQSST"
BASE COUNT          807 a          529 c          605 g          940 t
ORIGIN      
        1 gccgtatggt cattgtagaa tggatccatc acccaatggg cggcctgctc gtcaaaagac
       61 catatgtata agcggtaggt ggaataaata atcactatta ctaatgggat ggcatacatc
      121 caactgataa tcctgatgtt gttaagcttt ttaaataata tgactgcaac aaaataaaaa
      181 ggaactacaa accataaacg ggaaataaga aacttgattg aaaccagggg cagttcgctg
      241 gtaagcgctg ttataaacat ccagagcagg ttgattatta tcgcaatggt aaccgggtgt
      301 ttcagcactt ttgtgtcgta ttgcctttca aaaacaagtt ttatgaaaaa gaaaatcagt
      361 accccgaaga gcaggggctc tgtgggcagc gacaacgaca gcctgtcgtc gaaatccatg
      421 agatttatgg ctaaaggtgt acaaaaagtg atgatgtaca atactttgtc taaagcaaag
      481 aaaaaaagca gcaaaaggat taaaacgatg gggattaacg aaaaccaata aaattcttta
      541 ataataaaat atatgttcag ggcaatgaag attgcactaa taacatatac ccatattagt
      601 tttgatttat tactcaagac actatttatt gagaaatttt ttccggtttt tgcgcaccct
      661 caatgattga gaacagcaac aaagaaagca aaaaggctga aactgttgaa atacatacaa
      721 taagccagcg gatgggatag gattttttgt cggcggcaac cggttgggta accacattgg
      781 cgtaagtcaa ttctttattc aggtcggata cggatttgtc gtattctttt ttcagggcaa
      841 tataagaatc aatcaggccc cacaggttgg catccagttg ccggaactca cctccgtatt
      901 gtttcaggtt gtttagcgta acggcgatgt cgtcagtttt gttgcctctg cctgcagtaa
      961 tgttttttac atatcctttg ctgtattctt caacctgaat attgtaatcc agaatgttgt
     1021 attcgttgcg cagcaattgc agcctttttt cgatgctgtc aatctgcttt ttcttatcct
     1081 ccatctggtc cccgtatagt tttaccactt cggcagtttt gctgcgttgc aggttgcggg
     1141 ctttgctgtt catcagattg atgaattcat tgactatggc acaggcctgt cccggttcac
     1201 ggtcgtaaac ctctatcaaa accgattcat attcagttct tttgatttcc acattatctt
     1261 ccagttcgct gattattttg cttctgtaat atttatcgac agtgtcaatt ttatactctt
     1321 tgttaaggtc aaatttgttt acaatgtcaa aaagcatatc ttccgatttt agcaattgca
     1381 gcatctgttc ggtgttggtt tctgacgaat agggcgaaat attggcaggg taaataacgg
     1441 cgtatgattt gtactttggt ttaataaaag caggcgctga aaaaacggaa gacaacacta
     1501 tcgctaacag ggtaatgatc agcaaatgcc atttccatcg taatatcagt tttattaaat
     1561 atgtgctttt aaaaaagttg ttcatatttt cgtcaaaata atctattaga ggatattgtt
     1621 tgttttcagg tgcgaaaaga gtttcatgaa gttttgtttt gtttataatt gcgtgttctg
     1681 gtgcggcatc gctgatgtca agggtcaggt tgaactttgg atgtcctccg gtttgttggt
     1741 cggaaatttt ttttttgaga aattctcaat gataatcaat gtcattatgg agatgaaaaa
     1801 caccgacact acagacaaca tcacgattaa ccaccggaca gggtaagact ttcgttctgc
     1861 tttaaacgct ttgtccacca caaacttgga gggcagatcc tgggtggcat ccactttggc
     1921 ttcttcgtat tttgctttaa tggcgcttaa ctgctttttt tcgagctcca gttcatcgcg
     1981 caacgataca taagcactgc cataatcgcc cagcaactgc agcctttctt caatggcgcg
     2041 tacagcttcc ctgttgtttt ttgataaggc gatggctaac tgtgtattgt acgcctcggc
     2101 ttgtgtctca taatcgttaa cccccatttt tcgtagtacg gtaagggaat cttcgaggga
     2161 cttaatatca ttttgtaatt ttttgtattc ggtttcaaca atctgaaaag ctttaatagc
     2221 gcgttctttc tgcattttgt ttttcaccga gtcgagctgg tcggagatat aattggcaat
     2281 gtcggcagct aattgagggt ctttatcgag tacggtaatc tctactgcca tatattctgt
     2341 gcgccggtag gtaatattgc tgttatattc ctcaaacagg cgtgtcattt tatattttga
     2401 atcgttttcg atttcataat gctccatcag gttgaatttt ttaattaccc gcgatcttat
     2461 ttcattggag cttaaaatct gtaaaagttg ttctgtttga gcttcttccc caaattcaag
     2521 gatgtcgagt tttccgccat agttgtcggc cagcagcgcc ttggaaatgg cgttggtgga
     2581 ggccgggaat aaaatgacgg aggacttgta tttaggggtg attagcagtg agataaccga
     2641 tgatactatg gccgcaacaa ggcagataat tatcaggggc tttctccatt tataaagaaa
     2701 gaaaaaaaag tttgtcgtat tgaaattagc ttgtctgata tcctgttcag ccataacctg
     2761 aattgtaatc tgttataaaa ttgagcaaaa gtaatttatt ttttttattt gacaacttat
     2821 tttttaggac aacatgctca aaaaaaggca gggcaggcat tattttctga ttagcatctg
     2881 g
//
LOCUS       DAOSFP010000312         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_33337_length_2859_cov_3.833809, whole genome shotgun sequence.
ACCESSION   DAOSFP010000312 DAOSFP010000000
VERSION     DAOSFP010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33337_length_2859_cov_3.833809"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1749
                     /locus_tag="PLI16_08715"
     CDS             100..1749
                     /locus_tag="PLI16_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HOH84678.1"
                     /translation="MLFGIFGYFGFRFITGKTENKLSVILMLVGFAAILLLHYVAFNH
                     AFEDVDDNASYMIAAKSLVDKGAPYYLYLPEMTPDTEGALGLPIMLIPFYLIWGMNYV
                     PMEALIYFLMVGVVIISFFLFKKMAGTNFAVLITILVATHPYLVSFSSIIMTEIPYIF
                     WSLLAIYLCLKFEEKQKINYLLLAATVIAILMTYLTRAIGAGFFAAAILYFFLKSNFF
                     KNIKEKSLPFYKDVAFKKFLLISALLVIVFVAYQLWIRSVGGASQAEALAKMSLYDQF
                     TKNLGLVWDVFSQNIFCGKLARVSVNPDYLQPVNFLWALILLVAMAGLVYSIIKKELA
                     GLYFVFMMALLLVGNVATQALVVSRYLIVFTPFMIYFLYQGIKVLIGLPDKKKQIAQL
                     AGILVFCFLLGNSFTGDTYNVQRSTQATLYTPSYKAFLDCATWAKDNLPKEAIVASRK
                     ERIFYIFSDGIRGYKHVSSNEIGRMQEEGVTMEQFQQKKLDKMAATNTDYVIIDTFNA
                     ASVQMIYPIIEKNPDKFKLLKIIGEEKTGACYVFQVIKWWK"
     gene            complement(1756..>2859)
                     /locus_tag="PLI16_08720"
     CDS             complement(1756..>2859)
                     /locus_tag="PLI16_08720"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOH84679.1"
                     /translation="INVTKVDSSRPPSKNSGFYYALPRTFIKVDVVVDKTEQIRGPYA
                     EYAEKYLGLTNVIKTNATGYEVSEYKLSTFAEPDPQQFYFVMLKNACSSKIDKFRLYL
                     DEAGVLFNTTAAPEKSIADNAVFISEEEGNVYPDIFKSYTDLNLFVKVDTIIEKVNVD
                     SVMIEKMTFKRSLVEKTPEQRAKEAADYIIKVKENRFNLISGFQEVNYDKETFKYMND
                     ELEKMENEYRKLFTGITFTKKISYSFIYLPQSGKPVDTVPLFRFSTLRGVLDITDVYG
                     EIVSLSIKKYGNTDSLQVYQTKKDSLKVAQHGFYYRVPDYAEVNLFHSKGRNVSGKFL
                     ISQYGVLSSMPATIRRFATYPATGAIKNVGIKK"
BASE COUNT          740 a          636 c          602 g          881 t
ORIGIN      
        1 catttttgcc aacgattttt ttaatgtcaa ggagctgtcc aatccttaca tcaaagcaca
       61 agcttcctgg taattaccct gcaattcatt atttacacca tgctgttcgg cattttcgga
      121 tattttggct tccgctttat taccggaaaa accgaaaaca aactttcagt aatacttatg
      181 ctggtgggat ttgcagctat attactgctg cattacgttg catttaacca tgcttttgaa
      241 gatgtggacg ataatgcttc gtatatgatt gccgccaaat cgctggtgga caagggggct
      301 ccttattacc tctacctgcc cgaaatgaca cccgataccg aaggcgccct gggcctgccc
      361 atcatgctta taccgtttta cctgatttgg ggcatgaatt acgttcctat ggaagcgttg
      421 atttattttt tgatggttgg ggtcgtcatc atctcgtttt ttcttttcaa aaaaatggcc
      481 gggacaaatt tcgcggtgct catcaccatc cttgtggcca cgcaccccta tctggtttcc
      541 ttttcgagca tcatcatgac cgaaataccc tatatcttct ggtcattgct ggcgatttac
      601 ctatgcctta aatttgaaga aaaacaaaaa atcaactacc tgttgctggc ggctacagtc
      661 atcgccatcc tgatgaccta tctcacgcgc gccattggcg ccggattttt tgcagccgct
      721 atcctttatt tctttttaaa atcgaatttc ttcaaaaata ttaaggaaaa aagcctgccg
      781 ttttataaag atgttgcttt caaaaaattc ttattgatat ctgccttgct ggttatcgtc
      841 ttcgtggcct accagttgtg gatacgttcg gttggtggag cttcacaggc agaggccctg
      901 gccaaaatga gcctctatga tcagtttaca aaaaatctgg ggctggtatg ggatgtgttt
      961 tcgcagaaca tcttttgcgg aaaactggcc cgtgtaagcg tcaaccccga ttacctgcag
     1021 ccggtaaact tcctgtgggc actcatactg ctggtggcta tggcaggctt ggtttacagc
     1081 atcatcaaaa aagaactggc cggcttgtat tttgttttta tgatggcttt actgcttgtc
     1141 ggtaatgtgg ccactcaggc gctggtcgtg tctaggtatc tcattgtttt cacgcccttt
     1201 atgatttatt tcctgtacca gggaataaaa gtgctgatag gtttgcctga taagaaaaaa
     1261 cagatagccc aactggccgg catcctggtc ttttgttttc tccttggcaa ttcttttacc
     1321 ggcgacacct acaatgtgca gcgttccacc caggccacac tatatacgcc atcctacaag
     1381 gcttttcttg attgtgccac atgggccaaa gacaatctgc ccaaggaggc tatcgtagcc
     1441 agccgcaaag aaaggatttt ttatattttc agcgatggga taaggggtta taaacatgta
     1501 tcgtccaacg aaataggccg aatgcaggaa gaaggcgtta ccatggagca gttccaacaa
     1561 aagaaactcg ataaaatggc ggcgaccaat accgattatg tcattatcga cactttcaat
     1621 gcagcatcgg tacaaatgat atatcctatc attgaaaaaa atccggacaa attcaaactg
     1681 ctgaaaatta tcggcgagga gaaaaccggc gcctgctatg tgtttcaggt cataaagtgg
     1741 tggaaataat accgattatt tttttattcc gacgtttttt attgcgcctg ttgcaggata
     1801 cgttgcaaaa cgcctgatgg tcgccggcat ggaagacaac acgccatatt gcgaaatcag
     1861 gaatttaccg ctgacattcc tgccttttga atgaaacaaa ttgacctcgg cataatccgg
     1921 caccctgtaa taaaatccgt gctgtgctac tttcagggaa tcctttttcg tctgatatac
     1981 ctgtaaggaa tccgtattac catatttttt tatactgagg gaaactattt cgccatacac
     2041 atcggtgatg tcaagcacac ccctgagcgt tgaaaaacga aacagcggaa cggtgtcaac
     2101 aggtttcccg gattgtggaa ggtaaataaa actgtacgat atctttttgg taaacgtgat
     2161 accggtaaac agtttacggt actcattttc catcttttca agttcatcgt tcatgtattt
     2221 gaaagtctcc ttgtcgtagt ttacttcctg aaatccgctg ataagattaa aacggttttc
     2281 tttcacttta ataatatagt cagcggcttc cttggcccgc tgttcgggtg ttttttcgac
     2341 caatgaccgt ttgaacgtca ttttttcaat catcaccgaa tccacattta ctttttcgat
     2401 gatagtatcg acttttacga aaaggttcag gtcggtgtag gacttaaaaa tatcaggata
     2461 cacattgcct tcttcttcag aaataaaaac agcattatca gctatggact tttctggtgc
     2521 tgccgtggta ttgaacagta cgcctgcctc atccaggtac agcctgaact tgtcaatttt
     2581 actgctacag gcgtttttca gcataacaaa ataaaattgc tgcgggtcgg gttcggcaaa
     2641 ggtggaaagc ttgtattcgc tgacttcata ccctgtggcg ttggttttaa tgacattggt
     2701 aagtcccagg tacttttcag catattcggc ataaggtccc ctgatttgtt cggttttgtc
     2761 aaccaccaca tccactttta taaaggtgcg gggcaaggca taatagaaac cgctgttttt
     2821 tgatggcggc ctgctgctgt caacctttgt aacattaat
//
LOCUS       DAOSFP010000313         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_33376_length_2856_cov_5.039272, whole genome shotgun sequence.
ACCESSION   DAOSFP010000313 DAOSFP010000000
VERSION     DAOSFP010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33376_length_2856_cov_5.039272"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLI16_08725"
     CDS             complement(<1..947)
                     /locus_tag="PLI16_08725"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOH84680.1"
                     /translation="MVFSSVLFLFFFLPITLLLYYITRKRYKNLVALLASVVFYAWGA
                     PLFVFVIFGSIICDFLFAKYIHKSEGLKKKLLLTAAISLNVGILIYFKYANFFVENLN
                     TLLYSFGFTEIQWVKIALPIGISFFTFHEMSYVIDVYRGVKPPMKNILNYALYILFFP
                     QLIAGPIIRFNEISDQIEDRRYQFTVDNHLLGFFRFVIGLAKKVLIANVLGEEADRIF
                     ALGFYDLTTPLAWLGILAYAFQIYFDFSGYSDMAIGLARMMGFIFPENFNNPYISQSI
                     TEFWRRWHMSLSRWMRDYLYISLGGNRVSVPRMYFNLAFV"
     gene            complement(952..>2856)
                     /locus_tag="PLI16_08730"
     CDS             complement(952..>2856)
                     /locus_tag="PLI16_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOH84681.1"
                     /translation="QMAVLTPPPIPVEMKITDDGLFYGTPVSEFDSIPITFKVTDNNN
                     IENTKTLSFSSEGVIATHSVSAGTDTIINAGETVLLSVALKNIGNSVFPNAQLKISSG
                     DIHVTITDSVEPGGNLAPDDSVVFQDAFSFVVDTAVTDNYIININLAVYNAADTFNYN
                     LPLRVRSLILDIGAISVADGYNNILEPGENASVLTEIINIGGTTAYQINGILSTNDPY
                     VSVISDSMYLASLVSGDTSLGFYVIHVANNVPDGHLVVFNLALSADGGYLTHKFFTIQ
                     IGGNGEDFETNDFSRFPWVTTTTGDSAWFVTTQLPFEGLYCTESGNISDNQQTSLAVT
                     LNILNSGTVSFYRKVSCEAHPGYTNYDYFAFFIDGTEMGRWDGQTGWIRSEFPVSSGV
                     HTFRWSYVKDYSVSTGEDCAWLDYIVFPPTIEPGSNVLQYPASLFKEQEVESVSTDSI
                     TFTNYSLTNPALYHCEISGFSANSTSTWLTPEVQYGCVDAYGSGSLKLIFNSHNLVPD
                     TYNCLVNLTYNFTDSVSIPVTFQVVNTSGVDENSPAGYNVQAFPNPFTNQITISFTLD
                     EPADIGIEIYDLNGKKIKTLASGELHAGIHQIPWDGTAENHSRVPAGIYYFRFIGEDA
                     AISQRVILIK"
BASE COUNT          829 a          634 c          683 g          710 t
ORIGIN      
        1 acaaaggcca ggttgaaata catcctgggc acggatactc ggttgccacc cagcgaaatg
       61 tacaggtaat cgcgcatcca ccggcttagc gacatatgcc agcggcgcca gaactcggta
      121 atgctctgcg agatataagg attgttgaag ttttcgggaa aaataaaacc catcatccgc
      181 gccagcccta tagccatatc agaataaccc gaaaagtcaa aatatatctg aaaagcatag
      241 gccaaaatcc ccagccaggc caacggggta gtaaggtcat agaatcccag ggcaaaaatt
      301 ctatcagcct cctcgcctaa tacattagct atcagtactt tttttgcaag ccctatcaca
      361 aacctgaaaa atccaagtaa atgattatca acggtaaact ggtatcgcct gtcttctatt
      421 tgatccgaaa tctcgttaaa gcgaatgatg ggcccggcga taagttgcgg aaaaaataaa
      481 atatataaag cgtagttcaa aatgtttttc atcgggggtt tcacgccgcg atacacatca
      541 atgacatagc tcatctcgtg aaaagtgaaa aatgaaatcc cgatgggaag agcaattttt
      601 acccactgta tctcggtaaa tccaaaggag taaagcagcg tatttagatt ttcgacaaaa
      661 aagttggcgt atttaaaata aattaagatg cctacattta gcgaaatagc agcagtaagc
      721 agcagctttt tcttcaggcc ttctgacttg tgtatatatt tggcaaaaag aaaatcgcaa
      781 attatagaac caaaaattac aaagacgaaa aggggcgccc cccaggcata aaacaccaca
      841 ctggccagca gggctaccag gtttttgtaa cgcttacgtg ttatgtaata caataacagg
      901 gttatcggga gaaagaaaaa caaaaagagg acgctgctaa aaaccatgga attattttat
      961 caggatgaca cgttgtgaaa tagcagcatc ctcaccgata aagcgaaaat aataaatgcc
     1021 ggcaggcacc ctgctgtggt tttcggcggt gccgtcccat ggtatttggt ggatacccgc
     1081 atgaagctcg cccgaggcca gcgttttaat ttttttccca ttcaaatcat agatttcgat
     1141 acctatgtcc gccggttcat caagagtaaa cgaaatggta atctgattgg tgaacgggtt
     1201 gggaaaggcc tgtacattat agccggcggg actgttttcg tcaaccccgc tggtatttac
     1261 cacctgaaat gtcacgggga ttgatacgct gtcggtaaaa ttataagtca ggttgactag
     1321 gcagttatag gtgtcgggca ccaggttgtg cgaattaaaa atcagtttca gcgagccgct
     1381 gccgtatgca tcaacacatc cgtactgtac ttcgggggtc agccaggtac tggtgctgtt
     1441 ggcagaaaaa ccgctgattt cacaatggta cagcgccgga ttggtaaggc tgtagttggt
     1501 aaaagttatg gaatcggttg agacagattc tacttcctgt tctttaaaaa gggaagcggg
     1561 atattgcaat acgttggagc cgggttcaat agtcggagga aacacaatat aatcgagcca
     1621 ggcacaatct tcgcctgtgc ttaccgaata atctttaaca taggaccacc ggaacgtgtg
     1681 tactccgctg ctaacgggaa attcagaacg tatccagccg gtctgcccgt cccatcggcc
     1741 catttcagtg ccatcgatga agaaagcaaa gtaatcgtaa ttggtatagc ccggatgtgc
     1801 ttcgcacgac actttccggt aaaaactaac cgtgccgctg ttgaggatat tcagtgttac
     1861 ggccagcgaa gtttgctggt tgtccgagat gtttcccgac tctgtacaat ataatccttc
     1921 gaaaggcagc tgcgtggtta caaaccaggc tgaatcaccc gtggtggtag tcacccaggg
     1981 aaaccggctg aaatcattgg tttcaaagtc ttcgccattg ccgccaatct gtatcgtaaa
     2041 aaacttatgt gtcaggtatc ctccgtcggc actcaatgcc aggttaaaga ccaccagatg
     2101 gccgtcgggg acattgttgg caacatgaat cacataaaag cccaaggagg tgtcgccgct
     2161 gaccaggctt gccaaataca tggaatcgct gataacggaa acatagggat cgttggtgga
     2221 taaaattccg ttgatctggt aggccgtggt acctccaata ttgatgattt cagtgagcac
     2281 agaggcgttt tcgcccggtt caagaatgtt attgtaacca tccgccacac tgatggcgcc
     2341 aatgtctaat atcagcgaac gcacccgcag cggcagatta tagttaaatg tgtcggcagc
     2401 attatatacc gccagattaa tgttgataat gtagttgtcg gtaaccgcgg tatccaccac
     2461 aaatgaaaac gcatcctgga aaaccaccga atcgtcggga gccagattgc cgccgggttc
     2521 cacgctgtcg gtaatggtca cgtgtatgtc gccggaagaa attttcaatt gtgcgttggg
     2581 aaaaacactg ttgccgatat ttttcaacgc gacgcttaac aacaccgttt cgccggcatt
     2641 gattatcgtg tcggttccgg cgctgacact atgggtggca atcacgcctt cggaagaaaa
     2701 cgacaaggtt ttggtgtttt caatattgtt gttgtcggtt actttaaaag ttattggaat
     2761 gctgtcaaat tctgaaacag gggttccata aaacaaaccg tcgtcggtaa ttttcatttc
     2821 aaccggtatg ggcggcgggg tgaggacagc catctg
//
LOCUS       DAOSFP010000314         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_33633_length_2842_cov_3.222103, whole genome shotgun sequence.
ACCESSION   DAOSFP010000314 DAOSFP010000000
VERSION     DAOSFP010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33633_length_2842_cov_3.222103"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2842)
                     /locus_tag="PLI16_08735"
     CDS             complement(<1..>2842)
                     /locus_tag="PLI16_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84682.1"
                     /translation="LGSESNAIFESDNYYNEFRWEKLRGMEEIHPLVELKQIADARGS
                     KTISELDVMTKFRVPKELAQGQLLTYAEKGFIIYNIDDGIVVLKDRIYNYINAKTGKT
                     DYDVIQFNSIISAQDNGTLNLMNYDLKLRGVARIHLSDSQFVYVDPYEQEIVLKKNRD
                     FEFDGRIHAGLFDFYGKKITFKYDMFRFDMPMTDSMSFKVQDRTKPTDLYGHHPLVKV
                     RTVIENLNGEMLIDHPNNKSGLKNFANYPSFISKKDAYVYYDKPFIFNKVYSRDRFYY
                     RLQPFRIDTLDSYNSERKQFNGYLVSAGIFPDIVEPLSVQPDYSLGFIIQTPPGGFKA
                     YGGKGTYDSIVDLSYKGFLGKGTLKYLNSTSRSDDFIFFPDSLYARAKEYTIKEKPTP
                     VEYPSVYAQDVDIKWYPYQDVMNVNQMTKPFEVFANKTLLKGGLSMSSKALTAHGLIS
                     FGKVEMSSENYLLKHHSFSSDTTNVQFKTPDLSEMVLRTENFNVAIDFNKQLGKFRSN
                     DENSKITFPFNYYACYMYNFDWLMDKDMLNFHSSKKADYAYLKTKTMQELIDEEFTDS
                     YFVSEHPAQDSLSFYAPDAFYHLTENIIYTQDVPIIHVADAGIFPDSGKVTIDKKAEM
                     LPLHNARLIANTDSKFHYIYNDTINILSKKIYRGNGFYDYVDELGDKQKIYLKRIEVD
                     KTGQTNAFGDISEESGFRLSPFFDFAGKVTMYADKEFLNFDGGSRIRQDCDTSKARWL
                     RFVADIDPEKVAIPITDKPKEYVSGATGNELGMGLYIGDDSTLVYPAFVKPKNNFNDG
                     DVITATGVMTYDKAQQRYYVGPVVDSSAKYIKPENYLYLDKQNCYIFGEGRVNMGANL
                     GRLVLESFGTVRNNIIDNSTYFDVAMNLDFFFEKDAYQMFAQAIINDGGLEPADPSED
                     KFYNAMRFMVGPKKADDLISQLSLSGKFKSIP"
BASE COUNT          697 a          602 c          683 g          860 t
ORIGIN      
        1 tccggtatgg atttgaattt tcccgacagg ctgagctggc tgatgaggtc gtcggctttt
       61 ttgggcccga ccataaagcg catggcgttg taaaatttat cttccgaggg gtcggcaggt
      121 tccagccctc catcgttgat gatagcttgt gcaaacatct ggtatgcgtc tttttcaaag
      181 aaaaagtcga ggttcatggc tacatcaaaa taggtgctgt tgtcaataat gttgtttctt
      241 actgttccaa aggattccag tacgagcctg ccgaggttgg cgcccatgtt tacccggcct
      301 tcgccgaaga tataacagtt ttgtttgtcg agataaagat aattttccgg cttgatgtat
      361 ttagccgagg agtcaaccac agggccgaca taatatcgtt gctgggcttt atcataggtc
      421 attaccccgg tggcagtgat tacatcacca tcgttaaaat tgttttttgg cttcacaaat
      481 gccggatata ccagcgtgga atcatcacca atatacaacc ccatgcccag ctcgttgccg
      541 gtggctcccg acacgtattc tttgggtttg tccgtaatgg gaatggctac tttttcaggg
      601 tcaatatctg ctacgaatcg caaccatcgt gcctttgagg tgtcgcagtc ctgcctgatg
      661 cgcgaaccgc catcaaaatt gagaaattcc ttatcggcat acatcgttac ttttccggca
      721 aaatcaaaaa acgggctcag cctgaatcct gattcttcgc tgatatcacc gaaagcattg
      781 gtttgtcctg ttttgtcaac ttctatacgc ttgagataaa ttttttgttt gtcacccaac
      841 tcatccacat aatcgtaaaa tccgttacca cggtagatct tctttgataa aatgttgatg
      901 gtgtcgttgt aaatataatg aaatttcgaa tcggtgttgg caatcaggcg ggcattgtgc
      961 agcggcagca tttcggcttt cttgtctatg gttactttgc ccgaatcagg gaaaatgccc
     1021 gcgtcggcaa catgtattat gggtacatcc tgagtgtaaa taatgttctc ggtgagatga
     1081 taaaacgcat cgggggcata aaaactcagg gagtcctgcg caggatgttc tgacacaaaa
     1141 taggaatcag taaattcttc atcaataagt tcctgcatgg ttttggtctt caggtaagca
     1201 tagtcggctt ttttgctgct gtgaaaattc agcatatcct tatccatcag ccagtcgaag
     1261 ttgtacatgt agcaggcgta atagttaaat ggaaaggtga tttttgagtt ttcatcgttc
     1321 gacctgaatt tgccgagttg tttgttgaaa tcaatggcca cgttgaaatt ctcggtacgc
     1381 aaaaccattt cgctcagatc gggtgtctta aactgcacgt tggtggtgtc cgacgaaaat
     1441 gaatgatgtt tcagcaggta gttttccgac gacatctcca cctttccgaa cgatatcagg
     1501 ccgtgggctg tgagcgcttt tgacgacatg gacaaaccgc ccttcaacag ggttttgttg
     1561 gcaaaaactt caaaaggttt tgtcatctgg tttacgttca taacatcctg gtagggatac
     1621 catttgatat cgacatcctg ggcataaacc gacgggtatt caacgggagt gggcttttcc
     1681 ttgatggtgt attcttttgc cctggcgtac agcgaatcgg ggaagaaaat aaaatcgtca
     1741 gaccggctgg tggaattgag gtatttgagg gtgcccttgc caagaaatcc cttgtagctg
     1801 aggtccacaa tggagtcgta tgtgcctttt ccaccatagg ctttgaatcc tcccggaggt
     1861 gtttgtatga taaatccgag ggaataatcg ggttgcacgg aaagcggttc gacaatgtcg
     1921 gggaagatgc ctgccgatac gagatagccg ttaaattgtt ttcgttcgct gttgtagctg
     1981 tctaatgtgt cgatacggaa tggctggagc cggtaataaa aacgatccct cgaatatact
     2041 ttgttgaaga taaatggttt gtcgtaataa acgtaagcgt cttttttact gataaaggag
     2101 gggtaattgg caaaattttt caggcccgat ttgttgttgg gatggtcgat gagcatctcg
     2161 ccgttgaggt tttcaatgac ggtgcgtact tttaccagcg gatggtgccc gtaaaggtct
     2221 gtgggcttag tgcggtcctg caccttgaaa ctcatcgagt cggtcatcgg catatcaaag
     2281 cggaacatat catatttgaa cgtgattttt tttccgtaga aatcaaataa accggcgtgt
     2341 atcctgccgt caaactcaaa atcacggttt tttttgagta caatttcctg ctcgtaggga
     2401 tccacatata caaactgcga gtcgctaaga tgaatacgtg caacgccacg tagtttcagg
     2461 tcgtaattca tcaggttcag ggtgccgttg tcctgtgccg agatgataga gttgaactgg
     2521 ataacatcgt aatcggtctt gccagttttt gcgttgatgt agttataaat gcggtctttc
     2581 agcacaacga tgccgtcatc gatgttatag ataatgaagc ctttttccgc atatgtcaac
     2641 aactggccct gagccaactc cttaggaacc cggaattttg tcattacatc cagttcgctt
     2701 attgtttttg agcccctggc atcggctatc tgttttaatt ctaccagggg atgtatttct
     2761 tccattcccc tgagtttttc ccagcgaaac tcattgtagt agttatccga ttcgaaaatg
     2821 gcgttgcttt cgctacccag gc
//
LOCUS       DAOSFP010000315         2829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_33866_length_2829_cov_3.290916, whole genome shotgun sequence.
ACCESSION   DAOSFP010000315 DAOSFP010000000
VERSION     DAOSFP010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33866_length_2829_cov_3.290916"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLI16_08740"
     CDS             <1..259
                     /locus_tag="PLI16_08740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008665944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH84683.1"
                     /translation="FYIVQGKVYTNQDLDFFEQQMNKKFTAEERTAYTTVGGTPHLDG
                     AYTVFGEVVEGFDVINKIAAVKTQPGDKPVEDVTMEIIEIK"
     gene            318..1340
                     /locus_tag="PLI16_08745"
     CDS             318..1340
                     /locus_tag="PLI16_08745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOH84684.1"
                     /translation="MEHKIKTHLDEIASFSTTKADELEQFRIKYLGKNGILNAFFEEF
                     KAQPPQQKQVLGKLLNSLKQAASEKYATLKNELDAMGQQDIKPLDLTLNATSGAYGAR
                     HPMSIIRQEIIEIFSRLGFTVTDGPEIEDDWHNFTALNFQPDHPAREMQDTFFIEQNP
                     DIVLRTHTSSVQVRLMQNQKPPLRALMPGRVYRNEAISARAHCFFNQVEGLYIDKNVS
                     FADLKQTLLYFSKEMFGADTKIRLRPSYFPFTEPSAEMDISCTLCGGKGCPMCKKTGW
                     VEILGCGMVDPNVLENCNIDSKVYTGYAFGMGIERITNLKYTVKDLRLFSENDIRFLK
                     QFENNI"
     gene            complement(1548..>2829)
                     /locus_tag="PLI16_08750"
     CDS             complement(1548..>2829)
                     /locus_tag="PLI16_08750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84685.1"
                     /translation="GNIENLDDEQLAYLNSLLVNFSNIFFTDINVFNAQGFLAATSRP
                     QIFSEALLSDRMNPTAFNLLNGENKTTFIHKENIGELKYYSAYVPFFGMNNNVLYYIN
                     LPYFAKESELNRELSSFLSAFINIYVVFIALTIIIALITGSRITRPLNVIKEKIRNVK
                     LEKKNEKIVWERNDEIGGLISEYNRMIDELAESAEKLSRSERESAWRVMAMQVAHEIK
                     NPLTPMKLSIQHLERSWKDKTPDWDEKLERFTKNIIEQIDNLSAIASEFSYFAKMPKP
                     VNEKINLSELLTNSVSIFMANTHIDILFEKNKIPRDCFVFADRNQIIRVMNNILTNAI
                     QAIGKDQNGFIEIGLNKLEERAVVFVRDNGSGISEEMKPRIFTPNFSTKSEGMGLGLA
                     IVKTIIENSGGKIWFESEVKKGTTFYIELPLYIE"
BASE COUNT          789 a          622 c          588 g          830 t
ORIGIN      
        1 gttctatatc gtccagggaa aagtttacac caaccaggac ctcgacttct ttgaacagca
       61 gatgaacaaa aagtttactg cggaagaacg caccgcttac accaccgttg gcggcacgcc
      121 gcatcttgat ggcgcataca ccgtttttgg cgaagtggtg gaaggttttg atgtgataaa
      181 caaaattgct gccgttaaaa cccaacccgg cgacaaaccc gttgaggatg ttaccatgga
      241 aattattgaa ataaaataat catttctcca gacacctcct tttctgaagc cggcattacc
      301 tgcataatac tacaaaaatg gaacataaaa taaaaactca tcttgacgaa atagcctcat
      361 tttcaacaac aaaagccgac gaacttgagc aattccgaat caaatactta ggaaaaaacg
      421 gcattctcaa cgcttttttc gaagaattca aggcccagcc cccgcagcaa aaacaggtgt
      481 tgggaaaact gttaaattct ttgaaacagg ctgcatccga aaaatacgct accctgaaaa
      541 acgaactcga tgcgatgggc cagcaggaca tcaagccgtt ggacctgaca ctcaacgcta
      601 ccagcggagc ctatggtgcg cgccatccca tgtcgataat tcgccaggaa atcattgaga
      661 ttttttcgag attaggtttt accgtaaccg acggccccga aatagaggac gactggcaca
      721 atttcacggc acttaatttc cagcccgacc atccggcgcg cgaaatgcag gatacctttt
      781 ttattgaaca aaatcctgat atcgtgttgc gtacacacac ttcttctgta caggtccggc
      841 taatgcagaa ccaaaaacca cccctgaggg ccctgatgcc gggcagggtt taccgcaacg
      901 aagccatttc ggcgcgtgca cattgttttt ttaatcaggt ggaaggctta tatatcgata
      961 aaaatgtgtc ttttgccgat ttaaaacaaa cactgcttta cttttcaaaa gaaatgtttg
     1021 gcgccgacac taaaatacgt ttacggcctt catacttccc ttttaccgag ccttcggccg
     1081 aaatggatat atcctgtaca ttatgcggcg gaaaaggatg tcctatgtgt aaaaaaaccg
     1141 gctgggttga gatattgggc tgtggcatgg ttgaccccaa tgtgctcgaa aactgcaata
     1201 tcgatagcaa ggtttatacg ggctatgctt ttggaatggg tatcgaacgt atcaccaatc
     1261 tgaaatatac cgttaaagac ttgcggctct tttccgaaaa cgacatccgt tttctgaagc
     1321 agtttgaaaa caatatttag tgtttgtaag aaaacgccaa ttttttcccc gcctgcatcg
     1381 ggcagggtta cgctttcgac aaaaatgctc atttacacca gtaaactccg ctttttgtcg
     1441 cttcgcaagc tcgcctgact ggcgcagtcg ggcaggcctc aaacttgacg ttttctttgt
     1501 acaaacatga gtttattatt aatactattt aattgttttt caacgcttta ttcaatataa
     1561 agcggaagct ctatgtaaaa tgttgttcct tttttcactt cggattcaaa ccagattttg
     1621 ccacctgagt tttcaatgat agtcttaact atggccaatc ccagtcccat accttcggac
     1681 ttggtggaaa aattgggtgt aaaaatccgc ggcttcatct cttcggaaat cccagagccg
     1741 ttatctctca caaatactac tgccctttct tccagtttgt taagaccaat ttcgataaac
     1801 ccgttttggt cttttccaat ggcctgaatg gcattggtca ggatgttgtt cattacacgg
     1861 atgatttgat ttctatcggc aaaaacaaaa caatcccggg ggattttgtt tttttcgaac
     1921 aggatgtcaa tgtgcgtatt ggccataaaa atcgacaccg agtttgtcaa cagttcggaa
     1981 aggtttattt tttcgtttac cggtttgggc attttggcaa agtaagaaaa ttcggaagca
     2041 atagcggaca gattatcaat ttgttcgata atatttttgg taaagcgttc cagcttttca
     2101 tcccagtcag gagttttatc tttccatgat ctttccaggt gctgaatgct gagtttcatg
     2161 ggtgtcagcg gattttttat ctcgtgggcc acctgcatgg ccatgaccct ccaggcgctt
     2221 tcgcgttcgg aacggcttag tttttcggca ctttcggcca gttcgtcaat catgcggttg
     2281 tattctgaaa tcaggccccc tatctcatcg ttgcgttccc agacaatttt ttcgttcttt
     2341 ttctcaagtt tcacattcct gattttttcc ttaataacat tgagcgggcg ggtaatccgg
     2401 ctaccggtaa tcaaggcaat tattattgtt aatgcaatga ataccacata tatattgata
     2461 aatgccgaaa gaaaagatga taattccctg tttaattcgc tttcttttgc aaaatatggt
     2521 aaattgatgt aatacagtac attattgttc attccgaaaa atggcacata ggcagaataa
     2581 tacttcagtt cgccaatatt ctctttatga atgaatgtgg tcttgttttc accgttgagc
     2641 aggttaaagg ccgttggatt catcctgtcg gacaataggg cttcggaaaa gatttgtggg
     2701 cgcgaggtgg ccgccagaaa gccctgagca ttaaatacat tgatatcggt aaaaaatatg
     2761 ttggaaaagt tcaccaataa actgttgagg taagcaagtt gttcgtcgtc caggttttcg
     2821 atattgcca
//
LOCUS       DAOSFP010000316         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_34250_length_2807_cov_3.685683, whole genome shotgun sequence.
ACCESSION   DAOSFP010000316 DAOSFP010000000
VERSION     DAOSFP010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34250_length_2807_cov_3.685683"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..540)
                     /locus_tag="PLI16_08755"
     CDS             complement(172..540)
                     /locus_tag="PLI16_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84686.1"
                     /translation="MYSINYERKVFTRKFLSLAVRTGFSYFNLFGLSTELFDYHLNMI
                     TLFKLYKRKYFELGSGATYITEWDYNHSGKNSVFYIDYVFIAGLRIENFNGFQWRLCL
                     TPKFRSYDGRYLFGRLWGGH"
     gene            1157..2512
                     /locus_tag="PLI16_08760"
     CDS             1157..2512
                     /locus_tag="PLI16_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOH84687.1"
                     /translation="MNRKNVIIIGAAGRDFHNFNTFFRGNEAYNVVAFTAAQIPDIAG
                     RKYPKELAGNLYPDGIPIYVESDLPKLIKDLKVDICVFAYSDVPYNRVMGVSAIVNTA
                     GADFMLMGPKHTQVVSTKPLISVCAVRTGCGKSQTSRRVIEILMAKGLKVVAIRHPMP
                     YGDLNEQKVQRFATVEDLKKHKCTIEEMEEYEPHVVRGNVIYAGVDYEAILREAEKEA
                     DVIIWDGGNNDFSFYKADLSITVVDPHRPGNELSYYPGEVSLRMADAVVINKIDSASP
                     ENIQIVRNSIAKVNPNAVVIDGASPLTVDKPELIRGKRVLVVEDGPTLTHGEMKIGAG
                     TVAAMKFGAAELVDPRPYAVGKLAETFKIYPNIGTLLPAMGYGEQQVADLEKTIAATP
                     CDAVVIATPIDLNRVVKIQKPNAIVGYDLQEIGRPDLDDVIGDFLKKHNLDKGGCSCC
                     Q"
BASE COUNT          898 a          624 c          522 g          763 t
ORIGIN      
        1 acgattttag atttttgaag tgtaggaata cttctcagat catcattcgt taatcagtgt
       61 tcgatattaa aagggaataa atgatgtatg aattttccag acaggaactg cccgatcagc
      121 cttaatatag ccatcattgt tcaatattgc tgtcaaaagc tgtatcccac gctaatgccc
      181 cccccatagc ctaccaaata aataccggcc atcgtaagag cggaactttg gtgttaaaca
      241 caaacgccac tggaatccat tgaagttttc tatcctaagt cctgcaatga atacatagtc
      301 aatataaaaa actgaattct tcccggaatg attataatcc cattcggtaa tataggtagc
      361 gccggaaccc aactcaaaat atttcctttt gtaaagtttg aaaagtgtaa tcatgttaag
      421 atgataatca aacaattcag tactgagccc aaacaggtta aagtatgaaa accctgtgcg
      481 taccgccagt gataagaatt tgcgtgtaaa aaccttacgc tcataattga ttgagtacac
      541 gggcgcactg cccaataact caacaaaaaa ggtattgttc ctgattctac tttctctaat
      601 agaatccagt ttacggcgat ttctttcttc gtatgaaatt accttgacag ccttcgggct
      661 tttttaaggc atatgtttct ggttgaacaa atacgacgct acctcctcca tcccaatgga
      721 atcgttttgg gttttaaaag tagtttgcgt agtgtaaaga atactgttat tcctgatgta
      781 caatattttg caccagatag tatcctgatt tttttaatca tagcatcctc tctactctgc
      841 gcaaagcagc agtgtactta gataatcagc ccgaacaagg cgagaatggc ttttccgacc
      901 tatcttattc ccatgacata atggattact gtgcaaatat aaataattat cacaaagaac
      961 attttttaaa aagtacgcca tcggcattat ttataagttt atttctattt tttcagcatc
     1021 acagtatctt tgcccctgct ggcttataga ccaaaagcag tgttgttgaa aaaaatattt
     1081 ttatttcttt taataatgaa cctcttaaaa agttaccttt gccaaataca ataaactaac
     1141 cccaaaaaaa tatattatga atcgaaaaaa cgtaattatt attggtgcag caggtagaga
     1201 ttttcacaat ttcaatacct ttttcagagg aaatgaagct tacaatgttg tagctttcac
     1261 cgccgcacaa attcccgaca tcgccgggag aaaataccct aaagaattag ccggtaacct
     1321 ctatcctgat ggcattccaa tatacgtaga aagcgacctt cccaaactca taaaagacct
     1381 gaaagttgat atctgcgtgt ttgcatacag cgatgttccc tataacagag ttatgggtgt
     1441 cagcgccata gtaaatacgg caggcgccga ttttatgctg atgggcccca aacataccca
     1501 ggtagtcagc accaaacctt tgatttctgt ttgtgctgtg cgtaccggat gcggcaaaag
     1561 ccagacttca cgccgggtaa tcgaaatatt gatggctaaa ggtttaaaag ttgtggctat
     1621 ccgccatccg atgccttacg gcgaccttaa cgaacaaaaa gtacaacgtt ttgccaccgt
     1681 cgaagacctg aaaaaacaca aatgcactat cgaagaaatg gaagaatacg aacctcacgt
     1741 agtgcgtggc aatgtgattt atgccggtgt tgattatgaa gctatcctgc gcgaagcaga
     1801 aaaagaagcc gacgttatca tttgggatgg cggcaacaac gacttttcat tttacaaagc
     1861 tgatctttcc atcacggtgg ttgatccgca ccgtccaggc aacgaattga gctattaccc
     1921 cggcgaagtg tcgctgcgca tggccgacgc cgttgttatc aacaaaatag acagcgcctc
     1981 gcccgaaaac atccagatcg tacgtaacag catcgccaag gttaacccca acgctgtcgt
     2041 tattgatggt gcttctcctc ttaccgttga caaacccgaa ctcatcagag ggaaaagagt
     2101 gttggttgtt gaagacggtc ccaccctcac ccatggtgaa atgaagattg gcgcaggcac
     2161 cgttgccgct atgaaatttg gcgcagccga actcgttgac ccccgcccct atgctgttgg
     2221 aaaattagct gaaacattca aaatttatcc aaacattggt accttgcttc ctgcaatggg
     2281 ctatggcgaa caacaagtgg ccgacctcga aaagactatt gccgctacac catgcgacgc
     2341 agttgttatt gctacaccta ttgacctgaa cagagtggta aaaattcaaa aacccaacgc
     2401 catagtaggc tatgacctgc aagaaatcgg acgtcccgac cttgatgatg tcattggcga
     2461 tttcctgaaa aaacacaacc tcgacaaagg cggttgttct tgctgtcaat aaattaaagg
     2521 cgacgaaagt cgcctttttt tatgccccaa tcttaaatac aaccaataaa aaatatatat
     2581 ccgcaagcca ttcaggcaac cgaccgttgt taaaaaaaaa tgataaaata ttttcaaaat
     2641 agctcagaat tagcccaaaa cataataata ttgttatagc aatccattaa aaacccaata
     2701 ccataaaata aatgaaaaac aaaagtttaa tatccattga tgacctcaac aaagagcagt
     2761 acatcaaggt tcttgacacc gccgccactt ttgaaaaaaa tcctgtg
//
LOCUS       DAOSFP010000317         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_34331_length_2802_cov_4.556243, whole genome shotgun sequence.
ACCESSION   DAOSFP010000317 DAOSFP010000000
VERSION     DAOSFP010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34331_length_2802_cov_4.556243"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLI16_08765"
     CDS             <1..985
                     /locus_tag="PLI16_08765"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH84688.1"
                     /translation="VSYSIANYLGLNTELTNAIAIVHDLGHAPFGHEGEYILRKIVMD
                     EMGESFWHEKNSLFFVDKLETLPDLTNKDHNLNLTYAVRDGIISHCGEVNENAIFPRQ
                     ELIRLEDIERPNQYAPFTWEGCVVKIADKISYLGRDIEDALTLKILDKGQIAELSEIV
                     AGIKKVKVWQINNTILMNDFVINLCRMSTPEKGILFSEKYLELINSVKNFNYKHIYRH
                     KRLEYYGRLASLVINSIYSALAALYGPKNKLISTLDQQEKIYPSLIGAFKYWLMKYSD
                     IDLEERKTTKFGNQLVYDINNEKDYKMAILHYISSMTDHFAMKLFGELTRF"
     gene            1030..1728
                     /locus_tag="PLI16_08770"
     CDS             1030..1728
                     /locus_tag="PLI16_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84689.1"
                     /translation="MAKKNGKMKEIMDRFVTRSQIKQQVYQNTLHVFNELKSVVKDFA
                     VETQKDFKKGKIYNIPIEYKVKGDFETELTFAGDTLFFTMHTNVFEFPRDHSIMRSSY
                     LKEEPDRAYCGIIYVYNFLADSLRYNRENDIGYLVARIFVNKERHFVVEGKRQNEFLS
                     NSFVQESIDYPILRRILETAVLYCLEFDLLLQPYDMQKEITVSQIKEYSSSISITTGK
                     RLGFRFQADPEEQL"
     gene            1810..1883
                     /locus_tag="PLI16_08775"
     tRNA            1810..1883
                     /locus_tag="PLI16_08775"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1844..1846,aa:Glu,seq:ttc)
     gene            1902..2156
                     /gene="rpsT"
                     /locus_tag="PLI16_08780"
     CDS             1902..2156
                     /gene="rpsT"
                     /locus_tag="PLI16_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOH84690.1"
                     /translation="MANHISSKKRIRQTSTKRLHNRYYAITMRNALKQFRALKEKDKA
                     VAEMPKMVSMIDKLVTKDILHKNKAANLKSGMAKFCSSLK"
     gene            2249..>2802
                     /gene="radC"
                     /locus_tag="PLI16_08785"
     CDS             2249..>2802
                     /gene="radC"
                     /locus_tag="PLI16_08785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOH84691.1"
                     /translation="MSELRNIKQWAEDDRPREKLIAKGKAALSDAELLAILMRSGSQK
                     ETAVDLSKRILSDVSDNLLELSKLSLNDLMAYGGVGEAKALSIIAALELGKRCRVSEV
                     AIKEKISGSRDVFEYFQNNLADNQYEEFWVLFLNRANKIIKKNIISQGGIAGTVADPK
                     KIFKAALENNASSIILCHNHPSGN"
BASE COUNT          921 a          534 c          598 g          749 t
ORIGIN      
        1 agtgagttac agcatcgcca attatctcgg gttgaacacc gagctgacca atgccattgc
       61 tatcgtccac gatttgggcc atgctccttt tggccacgag ggcgaatata ttctccgcaa
      121 aatagtgatg gatgaaatgg gcgaatcgtt ctggcatgag aaaaacagtc tgttttttgt
      181 ggataaactg gaaacgcttc ccgaccttac caataaagat cacaacctaa atcttaccta
      241 tgccgtgcgt gatggcatta tttcgcattg cggcgaagta aacgaaaacg ctattttccc
      301 caggcaggag ctgatcaggc tggaagatat cgaaaggccc aatcaatatg ccccttttac
      361 ctgggagggc tgtgtggtga aaattgccga taaaatttcg tacctcgggc gggatattga
      421 agatgcgctg accttgaaga tccttgacaa agggcagatt gccgaacttt cagagattgt
      481 tgcggggata aaaaaagtga aggtctggca gataaacaac accatcctga tgaatgattt
      541 tgtaatcaat ctttgcagaa tgagcacgcc cgaaaaaggc attttgtttt cggagaagta
      601 ccttgaattg atcaattccg taaaaaattt caattataaa cacatctacc ggcacaaacg
      661 tctggagtat tacggacgcc ttgcttcgct ggtgattaac tctatttatt cagccctggc
      721 agccttatac ggccccaaaa acaaactgat aagcactctc gaccagcagg agaaaattta
      781 tccttcgctg ataggggctt ttaaatactg gctgatgaaa tattccgaca tagacctcga
      841 agaacgcaaa accacaaagt ttggcaatca gttggtgtat gatatcaata atgaaaaaga
      901 ttataaaatg gcgattttgc actacatatc ctctatgaca gatcatttcg ccatgaaact
      961 gtttggcgaa ctgacacgtt tctgacagtt ttcaggaaat taatacattt gcaaaaaaag
     1021 taagttgata tggcaaagaa aaatggcaaa atgaaagaaa ttatggaccg ttttgtgacc
     1081 aggtcgcaaa ttaaacagca ggtctatcag aatacgcttc atgtatttaa tgaattgaaa
     1141 tcggtagtaa aagattttgc agttgaaaca cagaaagact ttaaaaaggg taagatttac
     1201 aacataccaa tagaatacaa agtaaaagga gattttgaaa ctgaacttac ctttgcaggt
     1261 gatacgttgt tttttaccat gcataccaac gttttcgagt tcccgcgcga ccatagcatt
     1321 atgcggagtt cctatctgaa agaagaacct gacagggcgt attgcggaat aatttatgtg
     1381 tacaattttc tggcagattc attgcgttat aaccgcgaaa atgatatagg atacctggtg
     1441 gcccgtattt ttgtaaacaa agaacgtcat tttgtggttg agggaaaaag gcagaatgag
     1501 tttttaagca attcctttgt acaggaatca atagattacc caatactaag gcgaattttg
     1561 gaaactgctg tgctgtactg tcttgaattt gacctgttgc tacagccgta tgatatgcag
     1621 aaagaaataa ccgtgagcca gatcaaggaa tattcatcaa gtatcagtat aaccacaggc
     1681 aagcgacttg gtttccgttt tcaggccgac ccggaagaac agctatagaa agaaaaatat
     1741 tgaaaaaaat tttttgcaat aaaaaataat cgtatctttg caccctcaaa aattgagaga
     1801 aaaacatccg gcccgttcgt ctagtcggtt aggacgccag gttttcatcc tggaaatcag
     1861 gggttcgagt cccctacggg ctgcaaacaa aattttttaa aatggctaat catatttcat
     1921 ccaaaaaaag aatcaggcaa acaagcacca aaagattgca caaccgttat tatgctataa
     1981 ccatgcgtaa tgcattaaaa caattcagag cgctgaaaga aaaggataag gcagttgctg
     2041 aaatgcctaa aatggtttcc atgattgata aacttgtcac caaggatatc ctgcataaga
     2101 acaaagccgc taaccttaaa tcaggtatgg ccaaattttg cagctccctg aaataatttt
     2161 tttttttaat atttttttta acccttgtca caagcaaggg tttttttgtg aataaaatta
     2221 tataatttta cataaaaaaa tatacgctat gtctgaattg agaaatatca agcaatgggc
     2281 cgaggacgac cgtccccgcg aaaaacttat tgccaaagga aaagcggcat tgagtgatgc
     2341 cgagttgctg gcaatactga tgcggtcggg ttcgcaaaaa gaaactgccg tagatctttc
     2401 taaaagaatc ctgtcggatg tttcggacaa tctgcttgaa ttatcaaaac ttagcctgaa
     2461 tgatttaatg gcatacggcg gcgtgggtga agccaaggcg ctgagtatta ttgccgccct
     2521 ggagcttggc aaacgttgcc gtgtgtcgga agtggccatc aaagaaaaaa tttcaggcag
     2581 cagggatgtc tttgaatatt ttcagaacaa cctggccgac aatcaatatg aagaattttg
     2641 ggtgttgttt ctcaacaggg caaataaaat aataaagaag aacatcatca gtcagggagg
     2701 catcgcgggt acggtggccg atccgaaaaa aatttttaaa gcagccctcg aaaacaatgc
     2761 ctcctcgata atactttgtc ataatcatcc atcaggaaat at
//
LOCUS       DAOSFP010000318         2789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_34555_length_2789_cov_5.837601, whole genome shotgun sequence.
ACCESSION   DAOSFP010000318 DAOSFP010000000
VERSION     DAOSFP010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34555_length_2789_cov_5.837601"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLI16_08790"
     CDS             complement(<1..471)
                     /locus_tag="PLI16_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84692.1"
                     /translation="MRKFTLLSVMLMFCVSVFAQNASMDLKKYKSQIKPQAAKPTLDQ
                     AKAVIWQNDFSVPANWTIAHNTGTTGDWVIGTGGPSGSYAIDPIASTTAANGFALFDS
                     DLLCSGNQIANITTATSFSCAGHPNVKLSFQEMYRRYYDSTFVYVSNNGTTWTKF"
     gene            complement(610..1266)
                     /locus_tag="PLI16_08795"
     CDS             complement(610..1266)
                     /locus_tag="PLI16_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84693.1"
                     /translation="MCFSAEASFVAATAIAAVGIVSIKKSTSPAQRAFAVIPLFFAMQ
                     QFLEGWIWIVLANPNYATYKQFLTIGYLIFVWVVWPLYVPFSMGLLEKKPGRRKILYG
                     FLALGMVLTIICIYMLIFHHIEAVEARLHIKYIIDDLPSFDWLYGILYLVPTTVSFFV
                     SSIKKIWVLGVILLSSYIFSFIVYAGNVLSVWCFFGAVTSVLILWYIIQMKKKPETSE
                     "
     gene            complement(1298..1894)
                     /locus_tag="PLI16_08800"
     CDS             complement(1298..1894)
                     /locus_tag="PLI16_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84694.1"
                     /translation="MKKNFFLLAIIVIATAALPSCKKGENDPALSLLSRKARLVGDWV
                     LTGGSVTTLYSDGDTEITIYNGSERTIVTNGVTSNTTVYTKKFSIFKDGSFTKSTTDD
                     NDIYSSEGAWYFCPKNKELDLKSKESFAFSTTKQYYSLDGGPQHEITMEGTHAVGYPE
                     VMQIDRLTKNELVILVDDYYGTESGHYTATGTMTYKKM"
     gene            complement(1984..>2789)
                     /locus_tag="PLI16_08805"
     CDS             complement(1984..>2789)
                     /locus_tag="PLI16_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84695.1"
                     /translation="NGSCTSVASADVVINTQPVTPATPVVGTITQPTCAVATGSVVLS
                     GLPSGNWTINPGVIAGTGTTYTISGLGAGTYNFTVNNGSCTSVASADVVINTQPLPPT
                     VSLGHDTTICANQTVTLDAGNPGSGFLWSPDGQNTQVVTLDSLGLGIGPHTIIVTVTA
                     PNTCSASDTVVVTFDPCTDIPENTNDIILSIVPNPSNGLFFINVSGIEGPTELKIFSV
                     SGQLVFAEMIDGTGLTNKSINMEAYPTGMYFVRIVNNKTIYSEKIIIEK"
BASE COUNT          841 a          566 c          570 g          812 t
ORIGIN      
        1 aaatttagtc catgtagtcc cattgttact tacatataca aatgtactgt cataatatct
       61 ccggtacatt tcctgaaatg aaagcttaac atttggatgg cctgcacaac tgaacgatgt
      121 agccgtagtg atatttgcaa tttggttccc tgaacaaaga aggtcggaat caaataatgc
      181 aaagccatta gcagcagtag ttgatgcaat cggatcaatt gcgtaactac cagaggggcc
      241 tcctgttccg ataacccagt caccggtagt tccggtatta tgagctattg tccagttggc
      301 tggtacggaa aagtcgttct gccagataac agcttttgcc tggtcgagcg taggtttggc
      361 agcctggggt ttgatctgag atttgtattt tttcaaatcc atagatgcgt tttgtgcaaa
      421 aacagaaaca caaaacatca acataactga taaaagtgta aattttctca taagataatt
      481 ttttttgtaa aaatatataa aaacagatta attggcaaat tttttataaa cagaataatc
      541 taacatttta gagcttataa aaattatcta cttaggttat taataatccg atgttgtctt
      601 attggctccc tattctgaag tttcaggttt ttttttcatc tgaataatat accacagtat
      661 taaaacgctg gtaacggcgc caaaaaaaca ccataccgaa agtacgttcc cagcataaac
      721 tatgaaggaa aaaatatacg aacttagcaa tatcacgccc agtacccata tttttttgat
      781 gctcgaaaca aaaaatgaaa cggtggtagg caccagatat aaaatgccgt acaaccagtc
      841 aaaagaagga aggtcgtcaa tgatatattt gatatgaagc cgtgcttcaa ctgcttcaat
      901 gtgatgaaat attaacatgt atatgcagat tatggtcagc accatgccca gcgccagaaa
      961 accgtatagg atttttctcc ttcctggttt tttttcaagc agccccatag aaaaagggac
     1021 atataaaggc cagaccaccc acacaaatat caggtatcct atggttaaaa actgtttata
     1081 cgtcgcgtaa ttcgggtttg ccagcacaat ccatatccag ccctccagga attgctgcat
     1141 ggcgaaaaac aatggaataa ctgcaaatgc acgttgagca ggggaggttg atttttttat
     1201 tgaaacaatg ccaaccgctg caattgccgt ggcagctaca aaacttgctt ctgctgaaaa
     1261 acacataagt tgatatttat ttgaacaact tcgaaattta cattttctta taggtcattg
     1321 tgcccgtggc agtatagtgt ccgctttcag tgccataata atcatctacc agaatgacca
     1381 gttcattttt tgtcagcctg tcaatctgca taacttcagg atatcctact gcatgggtgc
     1441 cctccatagt tatttcgtgc tgtggtcccc catccaaact gtaatactgt ttggtggtcg
     1501 aaaaagcgaa cgattctttt gatttcagat ccagttcttt gtttttcggg caaaaatacc
     1561 atgccccttc cgatgaataa atatcattat cgtcggtagt tgatttggta aaagacccat
     1621 ctttaaaaat ggaaaatttc ttggtatata cggtagtatt tgatgtgacc ccgttggtca
     1681 caatggttct ttccgaacca ttgtagatgg tgatttcggt gtcgccatca ctgtacaagg
     1741 tagtaacgct accgccggta agcacccaat cacccaccag gcgggccttg cgtgaaagca
     1801 gcgaaagggc aggatcgttt tctccttttt tgcacgacgg caaagcggcg gttgcgataa
     1861 ctataattgc taacaggaaa aaattttttt tcataagtgt tgtttttttt gtgtacaaat
     1921 ttaaaaaaat aaaaagagac catctaataa tcgagacggc ctcttttcaa tttttaggtt
     1981 gtactatttt tcgataataa tcttttccga atatatggtc ttgttattca caatacgtac
     2041 aaaatacata cctgttgggt aggcttccat attgatggat ttgttcgtta aacccgtacc
     2101 atcaatcatt tcagcaaaaa ccaactgacc gcttacgcta aaaatcttaa gctctgtggg
     2161 tccttctatg ccgctgacat tgataaagaa caaaccattt gaaggattgg gtacaatgct
     2221 caaaataatg tcattagtat tttcaggaat atcagtacaa ggatcgaagg ttactacaac
     2281 tgtatctgaa gccgaacagg tattcggagc cgtaaccgtt acgatgattg tatgagggcc
     2341 tattccaaga ccaagagaat cgagcgtaac aacctgtgtg ttctgaccat cgggtgacca
     2401 taagaaccca gaaccaggat tgccggcatc aagagtaact gtctggttgg cacatattgt
     2461 tgtatcatgg ccaagtgata ctgttggcgg caagggctgt gtattgatta ccacatcggc
     2521 agaggcaaca gaagtacagg agccattatt gacggtaaag ttataggttc ctgcgcccag
     2581 tccgctgata gtgtatgttg ttcctgttcc ggcgataact cccggattga tggtccagtt
     2641 tcctgaaggt aatccgctta acaccacgct gcccgtagca acagcacaag tcggctgggt
     2701 tatagtgcca accacaggag tggcaggcgt aaccggctgt gtattgatta ccacatcggc
     2761 agaggcaaca gaagtacagg agccattat
//
LOCUS       DAOSFP010000319         2769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_34920_length_2769_cov_3.474208, whole genome shotgun sequence.
ACCESSION   DAOSFP010000319 DAOSFP010000000
VERSION     DAOSFP010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34920_length_2769_cov_3.474208"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="PLI16_08810"
     CDS             <1..276
                     /locus_tag="PLI16_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84696.1"
                     /translation="TITMKGVKDYIPAKSDKVRVPSLNGLWQLVPLAKGVTKIIYQVH
                     SESGGYVPEAIVNAFITDTPYYNLLNLKKIVESPLCPRSKIANLKEL"
     gene            369..1139
                     /locus_tag="PLI16_08815"
     CDS             369..1139
                     /locus_tag="PLI16_08815"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOH84697.1"
                     /translation="MKHTDDALILSCCESVHTFLAAHADAGNIKKYSRYFREGYDAWG
                     VDDKAMRAQRDLWYSQWKLQLNRQQMLALGDRLMAAGKYEESIIAINLLIQLQKDDKS
                     DILSRAGEWLEKFVVNWAQTDYMAGDAISPILLADPAAVDELLPWRKSASKWRRRAVP
                     VSLIKLMKQRGEPEKMLLIIDPMMNDAAREVQQGLGWFLREVWKKHPDAAEPFLLKWK
                     DTCGRLIIQYATERMDASQKILYKKSVGQKGSTIKKNK"
     gene            1142..2425
                     /locus_tag="PLI16_08820"
     CDS             1142..2425
                     /locus_tag="PLI16_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018966080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HOH84698.1"
                     /translation="MNEIICPNCKKVFKVDEAGFAEILKQVRDHQFEEELKNRLNLAE
                     KEKINAVKLAEANLRNSLQDALAQKDKEIIEMKARNERDLTTKLAQKEAEIAQMKSAI
                     EKSAIETKLTINQAVQKIEKERDELANLVKNKETEKALLEKSLTEKFSSALKTKDDII
                     KMKDEEIALRKDLKVRLSTKMIGETLEQHCETEFNKLRATGFQKAYFEKDNDARSGSK
                     GDYIYRETDDTGNEIISIMFEMKNEGDETATKKKNEDFFRELDKDRNEKKCEYAVLVT
                     LLEAENELYNAGIVDVSHKFPKMYVVRPQFFIPIITLLRNAALNSMRYKAELALVKNQ
                     NIDITNFEENIRIFKEGFARNYELASRKFQTAIEEIDKTIDHLQKTKEALLSSENNLR
                     LANNKAEDLTIKKLTRGNPTMTAKFEELSKKPGND"
     gene            2465..>2769
                     /locus_tag="PLI16_08825"
     CDS             2465..>2769
                     /locus_tag="PLI16_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84699.1"
                     /translation="MKILFHILLVFTFFSASMAQTTSKDYVCRKETKELWRELADSGR
                     YHEAINILMDSIQTSKQKNKKSAYWHVGQLYACNNEYEVAIEYLKKSKSRPTSLNPKT
                     "
BASE COUNT          933 a          571 c          666 g          599 t
ORIGIN      
        1 accattacga tgaaaggcgt taaggattac atccctgcca aatccgacaa agtgagggtg
       61 cccagcctga acggtttgtg gcaactcgta ccgctggcaa aaggagtaac aaagatcatt
      121 tatcaggtgc attccgagtc gggtggctat gtgcccgaag ccattgtcaa tgcctttatt
      181 actgatacac cctactataa tctgctcaac ctgaaaaaaa ttgtggaatc acccctgtgt
      241 ccccgttcga agatagcaaa cctgaaagaa ctctgaaaca ggccgccaac gactacggat
      301 gcaatagcag aaagcagcta aggcatatgg caggaaacac agtaaagacg gtacaaaaaa
      361 aacaatttat gaaacacacc gatgatgcac ttattttatc ctgttgcgaa agtgtgcaca
      421 cctttcttgc cgcacatgcc gatgcaggta acataaaaaa atattcccgt tattttcgtg
      481 aaggctacga tgcctggggc gttgatgata aggccatgag ggcgcagcgc gacttatggt
      541 atagtcaatg gaaactgcaa ctcaaccggc aacaaatgct ggcgctgggc gaccggctga
      601 tggccgcggg caaatacgaa gaaagtatca tagctataaa cctgctgata caactgcaga
      661 aggacgacaa gtcggatatt ttgtccaggg cgggggagtg gctcgaaaag tttgttgtca
      721 actgggcaca aaccgattat atggcgggtg atgcgatttc ccccatactg ttggccgatc
      781 ctgcagcggt ggacgaactt ttgccctgga ggaaatctgc ctcgaaatgg cggcggcgtg
      841 ccgtgccggt gtcgctcatc aaattgatga aacaaagggg cgagccggaa aaaatgctac
      901 tcatcatcga ccccatgatg aacgatgcgg ccagggaggt tcagcagggg ctggggtggt
      961 ttctgcgcga agtatggaaa aaacaccccg acgccgcgga gccttttcta ttgaaatgga
     1021 aggatacttg tggccgcctc ataatacaat atgccacgga acgcatggat gcctcccaaa
     1081 aaatattgta taaaaaatct gtcgggcaga aaggaagtac gattaaaaaa aataaatagt
     1141 tatgaacgaa attatttgtc cgaattgtaa gaaagtattt aaagtggatg aagccggctt
     1201 tgccgagatt ctgaagcagg tcagggatca tcagtttgag gaagaactga aaaacaggct
     1261 gaaccttgcc gaaaaagaaa aaatcaacgc cgtgaaactg gcggaagcca acctccggaa
     1321 ttcattacag gatgccctgg cacagaaaga taaggaaatc attgagatga aagcccggaa
     1381 tgagcgcgac ctgactacca agctggcgca gaaagaagcc gagatcgcgc agatgaaatc
     1441 agcaatagag aaatctgcca ttgagactaa actcacaata aatcaggcag tccagaaaat
     1501 agaaaaagaa cgggacgagc tggccaacct ggtgaaaaat aaggaaacag aaaaggcgct
     1561 gcttgaaaaa tccctcacgg aaaagttttc ctcggcccta aaaacaaaag acgatatcat
     1621 caaaatgaag gatgaggaga tagccctgcg aaaagatctg aaagtcaggc tttccacaaa
     1681 aatgatcggc gaaaccctcg aacagcattg tgagacagag tttaataagc tccgggctac
     1741 gggttttcaa aaagcgtatt ttgaaaaaga caacgatgcc aggtcgggaa gcaaaggcga
     1801 ttatatttac agggaaacag atgacacggg caatgaaatc atttcgatca tgtttgaaat
     1861 gaaaaacgaa ggcgacgaaa cggctacaaa aaagaagaat gaagattttt tcagagagtt
     1921 ggacaaagac cgcaacgaaa aaaaatgcga atatgccgtg ctggttacct tactcgaagc
     1981 cgaaaacgag ttgtacaacg cgggcattgt tgatgtatcg cacaagttcc cgaagatgta
     2041 tgtggtgcgc ccccagtttt tcatacctat cataacgctg ctgcgcaatg ccgccctgaa
     2101 ttccatgcgg tacaaagccg agctggccct ggtgaaaaat caaaatattg atatcacgaa
     2161 ttttgaagaa aacatcagga ttttcaagga ggggtttgca aggaattatg aactggccag
     2221 ccgtaaattt cagactgcca ttgaagaaat tgataaaacc attgatcatc tgcaaaagac
     2281 caaagaagcc ttattgtctt cggagaacaa cctgcgcctg gccaacaaca aggccgagga
     2341 cctgaccata aaaaaactca cgcgtggcaa tcctaccatg acggccaagt tcgaagagtt
     2401 gtcaaaaaag cccgggaatg attgaatcct aatgttgtgc ataataattt gagtcaatag
     2461 cagtatgaaa atattatttc atattttact tgtttttact ttcttctctg ccagtatggc
     2521 tcaaacaact agtaaggatt atgtctgcag gaaagagacg aaagaattgt ggagagagtt
     2581 agcagatagc ggaaggtatc atgaagcaat taatattttg atggacagta ttcagaccag
     2641 taaacagaaa aataaaaaaa gcgcctattg gcacgttggc caactttatg cgtgtaataa
     2701 tgaatatgag gttgccattg aatatttaaa aaaatctaaa tccagaccga catcactcaa
     2761 tcctaaaac
//
LOCUS       DAOSFP010000320         2766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_34976_length_2766_cov_4.447436, whole genome shotgun sequence.
ACCESSION   DAOSFP010000320 DAOSFP010000000
VERSION     DAOSFP010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34976_length_2766_cov_4.447436"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            507..1652
                     /gene="wecB"
                     /locus_tag="PLI16_08830"
     CDS             507..1652
                     /gene="wecB"
                     /locus_tag="PLI16_08830"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOH84700.1"
                     /translation="MIKLLTIVGARPQFIKASAISRTIRQDFWDKISEVILHTGQHYD
                     DNMSMVFFREMEIPLPLYNLEVGSGKHGKQTADMMAGIESILAKESPDAVLIYGDTNS
                     TLAGALAAAKMHIPVVHVEAGLRSFNKAMPEEINRIMADHVSTLLFSPTQKGIDNLLG
                     EGVRHSAAKPYNIDNPGVFHCGDIMYDNALYFSKVAEDKSTLLKDLRLKSGGYVLVTM
                     HRDTNTDDPVRLNAIFRAIQRISEKSDMKFVIPLHPRTRKKWNELPDDTLKDIIENNN
                     LITIIPAVSYLDFISLEKNARLIMTDSGGVQKESYFFKKPCLILRDETEWVELVEMGT
                     AILCGADEEKIVLSYKEVMNKKDLKFPEIYGDGQAARFILNEIIGNI"
     gene            complement(1736..>2766)
                     /gene="gdhA"
                     /locus_tag="PLI16_08835"
     CDS             complement(1736..>2766)
                     /gene="gdhA"
                     /locus_tag="PLI16_08835"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOH84701.1"
                     /translation="KFLSFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSL
                     MTELCKYIGPDTDVPAGDIGVGGREIGFMFGQYKRIRDEFTGVLTGKGLTWGGSLIRP
                     EATGYGQVYFANEMLKTRGMDLQGKTVVVSGSGNVAQHAAQKATQLGGKVVTLSDSGG
                     YIYDPDGITPEKLAYVMDLKNNKRGRISEYARKYNCEYVEGKTPWGVKCDIALPSATQ
                     NELNGDDARTLLANGCICVTEGANMPSTPEAIETFLASKILFGPGKAANAGGVATSGL
                     EMSQNSLRLSWTREEVDERLHNIMINIHKTCEKYGKESDGFINYVKGANIGGFVKVAE
                     AMLAQGLV"
BASE COUNT          744 a          663 c          608 g          751 t
ORIGIN      
        1 attggtaggc cccggattta ccttttatta cgccgataac gatgccgcaa ataattgcca
       61 ggatgaggat gatgaaatat cttttaataa acgaatagac agtggccaat agctggatga
      121 catcccggtt atcattatta gtttccttca tttttttttg tggtaaaatt aataaaattt
      181 tcaaacctgc ttttattatg tcaaaacagt tcgcagtttt tgatccccct atggtatttt
      241 tgatttttat tttagtaata atgagcacgt tcccataagt acttaatgtg aaaaatcagc
      301 cattttcctg ccccaaagcc tgcctgcgtg ccccatactg tggtaggcat ggacggctga
      361 ttttcagcaa ctttcctttg agattaaggg taaaaagtca ctgaaaatta ttaaatccga
      421 ctttttagtg gacttaataa tgttaaaccg gctcaaaccg gactcaatag tagtatattt
      481 gcaaaaacaa agcatctgaa ctgattatga taaagctact aaccatcgtc ggcgcccgcc
      541 cgcagtttat aaaggcttcc gccataagca gaaccatcag acaggatttt tgggacaaaa
      601 tttccgaggt gattttgcat accggtcagc attacgatga caatatgtcc atggtttttt
      661 ttcgcgagat ggaaattccc ctgccactgt ataacctcga agtgggctca ggaaagcatg
      721 gaaaacaaac cgcagatatg atggccggaa ttgagagcat actggcaaaa gaaagccctg
      781 atgcggtgct gatatacggc gacaccaact ccacgctggc cggcgcactg gcagcagcca
      841 aaatgcatat ccccgtggta catgtcgaag ccggcctcag gtcattcaac aaggccatgc
      901 ccgaagaaat aaaccgcatc atggccgatc atgtgtccac cctgttgttt tcgccaacac
      961 aaaaagggat cgacaacctg ctgggcgaag gagtgagaca tagcgctgca aaaccctata
     1021 acattgacaa tcccggcgtt ttccactgcg gcgacatcat gtacgacaat gcgctgtatt
     1081 tttcaaaagt tgccgaagat aaaagcacct tattaaaaga tctgcggctg aaaagcggcg
     1141 gttatgtgtt ggtaacgatg catcgcgaca ccaacaccga cgatcccgtg cggctgaacg
     1201 ccattttcag ggcgatacaa aggataagtg aaaaaagcga tatgaaattt gtcatcccct
     1261 tgcatccccg gacaaggaaa aaatggaatg aactgcccga tgacaccctg aaagatatca
     1321 tcgaaaacaa caaccttatt accattattc ctgcggtatc gtatttggat tttattagcc
     1381 ttgagaaaaa tgcccgcctg attatgaccg attccggcgg cgtacagaaa gaatcttatt
     1441 ttttcaaaaa gccctgcctt atcctgaggg atgaaaccga atgggttgag ttggttgaaa
     1501 tgggcactgc catattgtgt ggcgccgacg aagaaaaaat tgtgctttcg tataaggagg
     1561 tgatgaacaa aaaagacttg aaatttcctg aaatatacgg cgatggccag gctgcccggt
     1621 ttatcctgaa tgaaataatc gggaatatct gagcattccc aatcatttgt tgaagttttt
     1681 ataagtaaaa aagccgcacc ttttgggcgg cttttttatt ttttaaaaca gaccgctaca
     1741 ctaaaccctg tgcaagcatc gcttcggcaa cttttacaaa accgccgatg ttggcgcctt
     1801 tcacgtagtt gataaatccg tcgctttcct tgccatattt ttcgcaggtt ttgtgaatgt
     1861 tgatcataat gttgtgcagc ctttcatcca cttcttctct tgtccagctt aagcgcaggc
     1921 tgttttggct catttccagt cccgatgtag ccaccccccc tgcattggca gccttgccgg
     1981 ggccaaagag aatcttgctg gccaggaaag tttcaatagc ttccggtgtg gatggcatat
     2041 ttgccccttc tgtaacacag atacagccat tagctaaaag tgtacgcgca tcatcaccgt
     2101 taagctcgtt ttgggtggcg cttggcaggg ctatgtcgca tttcacaccc caaggggttt
     2161 tgccttccac atattcgcag ttgtactttc gggcatactc tgaaattctt cctctcttgt
     2221 tgtttttgag atccattaca taagccagtt tttcgggcgt aatgccatca ggatcataga
     2281 tatatcctcc cgaatccgac agggtaacta ccttgccgcc aagttgggtg gccttctgtg
     2341 cagcatgttg agccacattg cccgaaccgg aaacaacaac cgtcttgcct tgtaaatcca
     2401 ttccccgtgt tttcagcatc tcgttggcaa aatacacttg tccgtagccg gtagcttcgg
     2461 ggcgtatcag gctgccaccc caggtgaggc ctttcccggt aagcacgccg gtaaattcgt
     2521 cgcggattct tttgtattgt ccaaacataa agcctatctc acggccaccg acgccaatat
     2581 caccggccgg aacatccgtg tcggggccga tgtacttgca tagctcagtc atgaggcttt
     2641 ggcaaaaacg catcacctca ttatccgact tgcctttcgg atcgaagtcg gagccgccct
     2701 tgccgccacc cataggcaag gtggtcaggc tgtttttaaa cacctgctca aaggacagaa
     2761 atttta
//
LOCUS       DAOSFP010000321         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_35648_length_2731_cov_3.277280, whole genome shotgun sequence.
ACCESSION   DAOSFP010000321 DAOSFP010000000
VERSION     DAOSFP010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35648_length_2731_cov_3.277280"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLI16_08840"
     CDS             complement(<1..982)
                     /locus_tag="PLI16_08840"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84702.1"
                     /translation="MRKIFVFFLIMCWMVTAYSQSRILGLVTDAHSGNLLSGASVAIV
                     EMNTKTLTDVDGTYSFNTLQPGQYHVIVSYVGYKTDTTVVSLGRKTDRYLYHRFNTAV
                     VDMSEVSVTASRTAKGLSLPGRVDILTKTQMQEIPLVSVDDALMLVPGLNASRNYGIY
                     NKTGDVTLRGLNRNIHLLILLDGVPYSLFDGSSNLWNKLSPGKIENIEVLKGPNSALY
                     GGNAMAGVININTQKPQKPFEAKAKVFLGTYNTQGGEVTVGGNLAKNDKGFSWGAGGY
                     FRKSGGYVMVPDSLRTQYDVKTYLMEYNGNAKVAYHFAKGHNIEVQYEYSS"
     gene            complement(982..1842)
                     /locus_tag="PLI16_08845"
     CDS             complement(982..1842)
                     /locus_tag="PLI16_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84703.1"
                     /translation="MLLIIAVAFMISCNKNDGNPPSISLGTTAGFICSDTILPAGTNF
                     FVRIVAQKGDENITNLVIKNYHDGQQTVLFDTGVNNETIAITKILTKNVYQTENFTFM
                     VIDKNGKNASVSFIVNLDSSAGYQAVKVFDSVVLGAQKCNTTGSFMSLASGHVWFQAD
                     AATIPDSIEMLYYYDSTGDANTISSPNANIGSFIYPGATSPIYWPVRNETSFYKTTYS
                     LADFNAITNDSLLLAAYSGISAKRKAKNLVAGNVYSFKTIHTKYGMFAVEQVTGTDSG
                     TVRFTIKIQK"
     gene            complement(1925..2488)
                     /locus_tag="PLI16_08850"
     CDS             complement(1925..2488)
                     /locus_tag="PLI16_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84704.1"
                     /translation="MNTLSSPKTVSKAFPTKVIITIAEIILLVGIGALGVLLHARFRF
                     PFRMPGHWGVVYMALLFSGRLFSKKPYASSLSSAGAASMLLLPLGFSDPFMPVMYVLP
                     VFLVDVFYKLFGAKNKTVVFIALAAGLAYMTIPLFRIIISLATGVVYGSFLSGYYFPV
                     IMHFVFGACGGLIALGFFTLIKKLFKQ"
     gene            complement(2439..>2731)
                     /locus_tag="PLI16_08855"
     CDS             complement(2439..>2731)
                     /locus_tag="PLI16_08855"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862314.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOH84705.1"
                     /translation="LVHKGNIMKFTEGAFRNWGYDLAESEFASRVFTSRQWEKIKTTE
                     NQILFNFEKILLTEWWRPVLLQNHAVLFVEPVKDKDYEYIVISKDRIKSISN"
BASE COUNT          778 a          614 c          555 g          784 t
ORIGIN      
        1 cggatgaata ctcgtactgc acttcaatgt tgtgtccttt tgcaaagtga taggctactt
       61 ttgcattgcc attgtactcc atcaggtatg tttttacatc atactgggta cgtaatgaat
      121 ccggtaccat cacatacccg ccgctttttc tgaaatatcc gccggcaccc cacgaaaagc
      181 ctttatcatt ttttgcaaga ttacctccta cagtcacctc gccaccctga gtattatacg
      241 ttccgagaaa cactttggct ttagcttcaa agggtttttg tggtttttga gtgtttatat
      301 tgattacccc ggccatggca ttaccgccat acaaggccga gttaggccct ttcagcactt
      361 caatattctc aatttttccg ggagaaagct tattccatag attggaactg ccgtcaaaca
      421 agctgtaagg aaccccatcg agcaaaatca gtaaatggat gttgcggttg aggcctctta
      481 aggttacatc gccggttttg ttatagatac cgtagtttct tgacgcgttg aggcccggca
      541 caagcatcag cgcatcatca acagatacta aaggaatttc ctgcatctga gttttagtga
      601 gtatatccac cctgcccggc agactaaggc cttttgctgt acgtgaagcg gtaacggaaa
      661 cttccgacat atccaccacc gccgtgttaa acctgtgata gaggtaccgg tccgtttttc
      721 ttccaagact aacgactgtt gtgtcggttt tatacccgac atagctcact attacatggt
      781 attgacccgg ttgaagagta ttaaaagagt aggtaccatc gacatctgtt aatgtctttg
      841 tgttcatctc aactatggcc accgaagcgc ctgaaagcag gtttccgcta tgagcatctg
      901 ttaccaggcc aagtattctg ctctgcgagt aagcagttac catccagcac ataatgagga
      961 aaaagacaaa tatttttctc attatttctg aattttaata gtaaatcgta cggttcccga
     1021 gtcggtgccg gttacctgtt ccacggcaaa catcccatat tttgtatgta tggttttaaa
     1081 agaatacaca tttcccgcca caagattttt ggctttacgt ttggcactaa tcccgctata
     1141 ggctgccagt aataagctgt cattggtaat cgcattaaag tcggcaaggg aataagtggt
     1201 tttgtaaaaa ctggtttcat tccgcacagg ccagtagatg gggctggtgg ccccgggata
     1261 aataaatgag ccaatgttgg cgttgggtga agatatggta ttggcatctc ctgttgagtc
     1321 gtaataataa agcatttcaa tagagtcggg aatggtggct gcatcggcct gaaaccacac
     1381 atggccgctg gccagactca taaagctacc agtcgtatta catttctggg cgccaagcac
     1441 cacgctgtcg aaaactttta ccgcctgata accagcactg gaatcaagat ttacaataaa
     1501 agatacggag gcatttttcc catttttgtc aattaccata aaggtaaaat tttcggtttg
     1561 atatacattt tttgtgagga ttttggtaat agctattgtc tcattattaa cacccgtatc
     1621 aaacaatacc gtttgttgtc cgtcgtgata attctttatc acaagattgg ttatattttc
     1681 atcccctttt tgagcaacaa tccttacaaa aaaatttgtt cccgcgggca gtatggtatc
     1741 actgcagata aatcctgccg tcgttccaag gctgattgac ggaggattgc catcattttt
     1801 gttgcaggaa atcataaaag ccacagcaat aattaatagc aaaacagata actttatttt
     1861 tgttactacg tgcgatatat ttatgctgca gggcttttgt tttctataat tcatgcaaaa
     1921 gcgtttactg tttaaaaagt ttttttatca atgtgaaaaa tcccagggct atcaagcctc
     1981 cgcaggcgcc aaacacaaag tgcatgatta ccggaaaata atatcctgac aaaaatgagc
     2041 catagaccac accggtggcc aacgaaatga ttatcctgaa caggggtatg gtcatataag
     2101 caagtccggc cgccagtgca ataaaaacca cggttttgtt tttagcgccg aacaacttat
     2161 aaaaaacatc cactaaaaaa accggcaaca catacatcac gggcataaaa ggatcggaaa
     2221 atcccagcgg caagagcaac atggatgccg ccccggcact ggaaagactt gaagcgtatg
     2281 gtttttttga gaacagcctc cccgaaaata acaacgccat atataccact ccccaatgcc
     2341 ccggcatacg gaacgggaaa cggaatctgg catgaagcaa gacacccagc gcgccgatac
     2401 ctacaagcaa tattatttca gcaatcgtta tgattacctt agttggaaat gcttttgata
     2461 cggtctttgg agatgacaat gtattcataa tccttatcct ttacgggttc tacaaacaat
     2521 accgcgtggt tttgcaacaa tacgggacgc caccattcag taagcaaaat tttctcaaag
     2581 ttaaaaagaa tttgattttc tgtagtttta attttttccc actgccggct ggtaaaaacc
     2641 cggcttgcga attcactttc tgcaaggtcg tacccccaat tgcggaaagc tccttcggta
     2701 aacttcataa tgttaccttt atgcacaagt g
//
LOCUS       DAOSFP010000322         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_36015_length_2711_cov_4.053087, whole genome shotgun sequence.
ACCESSION   DAOSFP010000322 DAOSFP010000000
VERSION     DAOSFP010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36015_length_2711_cov_4.053087"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLI16_08860"
     CDS             complement(<1..616)
                     /locus_tag="PLI16_08860"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH84706.1"
                     /translation="MAYQEQLQLADSVDFDNEIPAPWQYKTNHNEAVKNRDEFKLLAL
                     SVDAEHLKKQMLLGETLPQIGVGAGYLYNNLMEKNNTNAVVFASVSVPISGWWEAMHT
                     LKKQKIQQQIVENERNDKNELLLLQMQQAWNAVEEAYSQIQLAKEAMNEARQNLKVTT
                     DYYRSGMKTISEVLEAQTLLQQAGNLYTDQAVQFRIRLTAYLQMV"
     gene            complement(626..1354)
                     /locus_tag="PLI16_08865"
     CDS             complement(626..1354)
                     /locus_tag="PLI16_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOH84707.1"
                     /translation="MKKTIITIIVLFAILPVKAQFSLDSCKKMAVANNAKVKNALLEI
                     EAAGQTKKAAFTRYFPHVSAAGSAFMSNESFIDVDLNDVDLNVTFEDQRLNEILQTLY
                     SNYGNYLPDATLNAQMMDKGIVAGVTAVQPVFAGGRIVHGNQLAKLGVEASHYKYNMA
                     EDGVLLKTEEYYWLVVSLKEKLKTIASAEKLLDTLYKDVNGAFESGVIIQNDLLKVKL
                     KQNELSSNRLKLENGIALATMGLC"
     gene            complement(1424..>2711)
                     /locus_tag="PLI16_08870"
     CDS             complement(1424..>2711)
                     /locus_tag="PLI16_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOH84708.1"
                     /translation="TSSPRFMATYAPNLPGKNFAQFIVNTNSNKETEDLLRDYSGKYA
                     NHFPNAIIRFKQINYQVARNPIEVRFTGDDIEQLHNESDKLVAFMHTLDGELSWIHTD
                     FDGYQPCAKIDLDQVEASRLGITKSMLAVDLATSFNGMPLTTLWEGDYAVPVKLKRDR
                     KSSDTDFQSLENEIIPTLIPGVWVPLRQVASITPDWQPTQIVRRNGVHSLTVSCDLKF
                     GGSEPKSLAKIKKYIDKTLVPQLPPDIKISYGGLEDTNKALIPEIIAGLCIAIMIIFF
                     FLLFAFKKISISILAIASSSFSLLGAIIGIWIFGIDFGITSVLGVVSLIGIIVRNAII
                     MFEYAEKLRTQKGYSAREAGLEAGKRRMRPIFLTSAAAAVGVIPMIISKSTLWMPMGV
                     VISFGMLFSVVLVVTILPVVYWQVYKNVKIKKVNTH"
BASE COUNT          688 a          613 c          590 g          820 t
ORIGIN      
        1 tcaccatctg caaatacgca gtcagcctga ttctgaattg aacagcttgg tctgtgtata
       61 ggtttccggc ctgctgtaac agcgtctgag cctccaatac ttcagaaata gttttcatgc
      121 cggaacgata atagtcggtg gtaactttca ggttttgcct ggcttcgttc atagcttctt
      181 ttgcaagctg tatctggcta taggcctctt caacagcatt ccatgcctgt tgcatctgaa
      241 ggaggagcag ctcattttta tcattccgct cgttttcaac aatttgttgt tgtatttttt
      301 gttttttcag ggtatgcatg gcttcccacc aacccgaaat tggcacgcta acgctggcaa
      361 aaacgacagc gttggtattg tttttttcca tcaggttatt gtacagatag ccggcgccaa
      421 cacctatttg tggcagggtt tcaccgagga gcatttgctt tttcaagtgt tctgcatcca
      481 cgctcagtgc aagcaattta aactcatcac ggttttttac ggcttcgtta tggttggttt
      541 tatattgcca gggcgccgga atctcattgt caaaatcaac agagtcggca agttgtaatt
      601 gttcctgata ggccaagcca atatactagc acaaccccat ggtggctaag gcgattccgt
      661 tttccaattt taaacgattg gagctaagtt cgttttgttt aagttttact ttcaacaggt
      721 cattttgtat aattactccc gattcaaagg cgccatttac atctttgtac aacgtatcca
      781 aaagcttttc ggcacttgct atggttttta gtttttcctt taacgaaaca accagccagt
      841 aatactcttc ggttttgagc aacacgccat cttcggccat gttgtatttg tagtgcgatg
      901 cttcaacacc gagttttgcc agctgattac catgcacaat cctacctcct gcaaaaaccg
      961 gctgaactgc tgtaacccct gccacaatgc ccttgtccat catttgtgca tttaaagttg
     1021 catcgggaag gtagttccca taattagaat aaagcgtttg cagtatctca ttgagtcttt
     1081 ggtcttcaaa tgttacattc aggtcaacgt cgttcagatc aacatcaata aatgattcgt
     1141 tcgacataaa ggcgcttccc gcagcgctga catggggaaa atacctggtg aacgccgcct
     1201 tcttagtctg ccctgccgct tcaatttcca ataaggcatt tttcactttg gcattattgg
     1261 ccacagccat cttcttgcaa ctgtcaagcg aaaactgtgc ttttacggga aggatggcaa
     1321 acaggactat aatagtgatt attgtttttt tcattttata ttcttaaaaa aatcaggtca
     1381 aataatattt ctacctgttt taagtatttt aatacctgtg ttcttaatgt gtatttacct
     1441 tttttatttt tacattttta taaacctgcc aatagacaac cggcaagata gttaccacga
     1501 gtacgaccga gaagagcatt ccgaaactga tcacgactcc cattggcatc caaagggtgc
     1561 ttttacttat gatcattgga ataacgccaa cagcggctgc cgccgatgtt aggaaaattg
     1621 gccgcatgcg gcgtttccct gcttccaggc cggcttctct ggcgctgtaa cctttttgag
     1681 tccttaattt ctctgcatac tcaaacatga tgatggcgtt acggacaata atgccgatca
     1741 ggctgaccac accaaggacc gaggtgatgc caaagtcgat gccgaaaatc cagatgccga
     1801 tgatagcgcc cagcaaacta aacgaactgg aggcaatagc cagaatagat atgctgatct
     1861 ttttgaatgc gaataacagg aaaaagaaaa tgatcatgat ggcaatgcaa agtcctgcaa
     1921 taatttccgg gataagtgct ttgttggtat cttccaaccc tccataagaa atttttatat
     1981 caggtggcaa ctgcggaaca agagtcttgt cgatgtattt cttgatttta gccaggcttt
     2041 tcggctcgct tccgccaaac ttcaagtcgc aggaaacggt aaggctatgt acgccgtttc
     2101 gccggacaat ctgtgtcggc tgccagtcgg gtgtgatgct ggccacctgg cgcaacggca
     2161 cccacacacc ggggatcagc gtggggatta tttcattttc gagcgactga aagtcggtat
     2221 cactgctttt gcggtctctt ttgagtttta cgggaacggc ataatcgcct tcccacagcg
     2281 tggttagcgg catgccgttg aaactggtcg ccagatcaac ggccagcatc gattttgtga
     2341 ttcccaaacg gctggcttct acctgatcca gatcaatttt ggcacaaggc tggtaaccgt
     2401 caaaatcggt atgtatccac gaaagttcgc cgtcaagcgt atgcataaaa gccaccagct
     2461 tatcagattc attgtgcagt tgttcgatat catcaccggt aaaacggact tcaataggat
     2521 ttctggccac ctgataattg atttgtttaa agcgaatgat ggcattagga aaatggttgg
     2581 cgtattttcc agaataatcc cggagcaaat cttccgtttc tttgtttgag ttggtattga
     2641 cgataaactg tgcgaaattt ttgccgggta ggttaggcgc ataggttgcc ataaaccggg
     2701 gagaactggt g
//
LOCUS       DAOSFP010000323         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_36016_length_2711_cov_4.022967, whole genome shotgun sequence.
ACCESSION   DAOSFP010000323 DAOSFP010000000
VERSION     DAOSFP010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36016_length_2711_cov_4.022967"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLI16_08875"
     CDS             <1..968
                     /locus_tag="PLI16_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOH84709.1"
                     /translation="YDFLNGMNDAGNSIATIVGTNVLPPKIAVLWAAFFNFVAFGIFY
                     GSPVAHKIGTGIIMPGTLEKDTVFILCSLLGAGSWVLICARNGIPNSVSHALIGGLIG
                     PALIKFGPAAVNVPNVLTIVLFIALAPLFGFIIGYFFMNLTLLIWGKKPPLKTDRYFR
                     VFQLLSSAAFSIGHGGNDAQKTMGIIFMLLLATGDPQYTSNPDHIPFWVALTCYTSIA
                     LGTLLGSGRIVKTLGRGLCHLKPIGGFCAETAGALTLFGVTLAGIPVSTTHTITGAIM
                     GVGVTKRASSVKWKLAANIVVAWVLTIPASMFFSALIYYVLKLFL"
     gene            1030..2301
                     /locus_tag="PLI16_08880"
     CDS             1030..2301
                     /locus_tag="PLI16_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOH84710.1"
                     /translation="MDVILDIVPKQLIDFLLVTVFSLLIGLSQRKLHPVSEDERLFGT
                     DRTFTFIGILGYILFIIDPEYLFLYMGGGLVLLVLLSVNYYHKITVFKDFGLTSIIIA
                     FITYCLAPLVITQPAWLYLLVVVTVLIFTELKETFTTFSHQIEKEEFITLAKFLIIAG
                     IVLPIFPNKPIVPFLEITPYKIWFAVVVISTVSYLSYLLKKYVFKKSGILISGILGGL
                     YSSTATTLILAKKSREAGVMKNNYASAIIFATAMMYLRIAILMLIFNAAMFRNSFVFL
                     LIMLLLTVVIGAIVYFYQHKEEESAAIATIKDKNPLEFKVALLFMVLFVAFTFVTYYA
                     VKYFGDMGLNVISIIVGVTDIDPFLISIFQGKINTSVDVLTRVSFQAIISNNILKLVY
                     SLIFADRKIRMPILIAFLIIIVINILLLFFI"
     gene            2403..>2711
                     /locus_tag="PLI16_08885"
     CDS             2403..>2711
                     /locus_tag="PLI16_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84711.1"
                     /translation="MEFEEQYARISVPSKKQSTLRNLCLVSLFFSGSMTLFSFGGFFF
                     SGWLSDYIQAFWQGFYGFGKSYLLLFFLGMFILFGSSFTGTLLMFRMKRPGYWIYLAA
                     N"
BASE COUNT          664 a          638 c          560 g          849 t
ORIGIN      
        1 tctatgactt tctgaatggc atgaatgatg ccggtaactc catagccacc atcgttggca
       61 ccaatgtgtt accgccaaaa atcgccgttt tatgggctgc ttttttcaat tttgttgctt
      121 ttggcatttt ttacggctca cccgttgccc acaaaatcgg caccggaatc attatgcccg
      181 gtacgttgga aaaagacacg gtttttattc tttgttcgtt gctgggcgcc ggctcatggg
      241 tattgatatg tgcccgaaac ggtattccca acagcgtttc acacgccctc atcggcggac
      301 ttatcggacc tgccctgatt aaattcgggc ccgcagcagt aaatgttccc aacgtactca
      361 ccatcgtatt gttcattgcc ctggctcccc tattcgggtt tataatcgga tattttttta
      421 tgaacctcac tcttttgatc tggggcaaaa aacctccgtt gaaaaccgac aggtatttca
      481 gggtattcca attgttatcc tcagcagcgt tcagcatcgg tcacggaggc aacgacgccc
      541 aaaaaactat gggcatcatt tttatgctcc tgctggctac cggtgacccg caatatacca
      601 gcaatcctga tcacataccc ttctgggtgg ccctcacctg ctatacctcc attgctctgg
      661 ggacactgct gggctccgga agaattgtaa aaacccttgg cagaggactc tgccacctga
      721 aacctatcgg gggattttgc gctgaaacag ccggtgctct tacccttttt ggagttaccc
      781 tggcaggtat ccccgtcagc accacacaca ctatcaccgg cgctatcatg ggtgtaggag
      841 ttaccaaaag agccagcagc gtaaaatgga aactggctgc caacatcgtg gtggcctggg
      901 tgcttaccat cccggcatcg atgtttttct cagcactcat ctattacgtg ctaaagttat
      961 ttttatagaa atctagcacc ggaaccagaa cctatgcagc attccggcgt ataaaagaaa
     1021 aaatatttca tggatgttat ccttgacata gttcccaaac agcttattga ctttttgctg
     1081 gttaccgtgt tttcgctact gataggctta tcacagcgaa aattgcaccc tgtcagtgaa
     1141 gatgaacggc tttttggcac cgaccgcact ttcacattta tcgggattct cggatatatt
     1201 ttgtttatca ttgaccccga atatttattt ctgtatatgg gcggtggact tgtgctgctg
     1261 gtgctgctgt ccgttaatta ttatcacaaa atcaccgtgt ttaaagattt cgggttgact
     1321 agcatcatca ttgctttcat tacctattgc ctggcgcccc tggtaattac ccaacccgcg
     1381 tggctctatc tccttgttgt ggttactgtg cttattttta ccgaactcaa agaaacgttc
     1441 acgacttttt cgcatcagat cgaaaaagaa gaatttatca ccttagccaa gtttctaatc
     1501 attgcgggca ttgtactgcc aatatttccc aataagccga tagtgccgtt tctggaaata
     1561 accccttaca aaatctggtt tgccgtagtg gttatctcca cggtttctta tttaagctac
     1621 ctgctgaaaa aatatgtatt taaaaaatcc ggcatcctga tttccggaat tttgggaggt
     1681 ctttacagca gcacagccac cacgctgata ctggctaaaa agagcaggga ggccggagta
     1741 atgaaaaaca attatgcctc ggccatcatt tttgccaccg ccatgatgta cctgcgcatt
     1801 gcaatactga tgctgatttt caacgcggcc atgttccgta acagttttgt ttttctgctg
     1861 atcatgctac tgcttacggt ggtcatcggc gcaattgtct atttttatca gcacaaagaa
     1921 gaagaaagcg ccgccatagc caccatcaag gataaaaacc ccctggaatt caaggtggcc
     1981 ttgctgttta tggtactttt tgtggctttt accttcgtta cgtattatgc tgtgaaatac
     2041 ttcggcgata tggggctaaa cgtaatctca atcatagtgg gggttaccga tatcgacccg
     2101 tttctgattt ccatttttca ggggaaaata aacacctctg ttgatgtgct caccagagtt
     2161 tcctttcagg ctattatcag taacaatatc ctcaaattag tctattcact tatctttgcc
     2221 gacaggaaaa tccgcatgcc catacttatt gctttcctga ttataattgt gatcaatata
     2281 ctgttgctgt tttttatctg acacccgaca gactattttt ctttggtaat aagtttggca
     2341 aattattcat agtgtattaa tttagccata aatttactta taatataccg tatctttcag
     2401 ccatggaatt tgaagaacaa tatgcgcgta tttccgtacc gtcgaaaaaa caaagtacac
     2461 taagaaattt atgcctggtc tcactgtttt tcagcggcag catgacactg ttttcctttg
     2521 gcggattctt cttttcgggg tggctcagcg attacataca ggcattttgg cagggatttt
     2581 atggttttgg taaaagttat ttattgcttt tttttctggg gatgtttata ctgtttggca
     2641 gttcatttac cggcacactg ctcatgttcc ggatgaagcg cccgggatac tggatttacc
     2701 tcgccgccaa t
//
LOCUS       DAOSFP010000324         2711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_36024_length_2711_cov_2.732304, whole genome shotgun sequence.
ACCESSION   DAOSFP010000324 DAOSFP010000000
VERSION     DAOSFP010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36024_length_2711_cov_2.732304"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PLI16_08890"
     CDS             <1..770
                     /locus_tag="PLI16_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOH84712.1"
                     /translation="LYKPFTLNGHPVIFMDIPSAEMTKYAANAMLATKISFMNDIANL
                     CEIMGANVNMVRKGIGSDSRIGNKFIYPGIGYGGSCFPKDVKALIKTADENKYSLRVL
                     KAVEEVNDDQKSVLFNKVMKYFNGQLKDKTIAMWGLSFKPETDDMREAPALVLIEKFL
                     QAGAKVKAYDPEAMKEAKRRLGNKIEFVNDQYEALIDADCLLIVTEWTEFRAPNFNVI
                     KKLLKKNVIFDGRNIYDLAVMKAEGFDYFCIGINTTK"
     gene            773..1702
                     /locus_tag="PLI16_08895"
     CDS             773..1702
                     /locus_tag="PLI16_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH84713.1"
                     /translation="MRKKILVTGGAGFLGSHLCERLLNEGNEVICLDNYFTGQKQNIV
                     HLLDNPYFELIRHDVTMPFFIEVDQIYNLACPASPIHYQYNPIKTIKTSVMGAINMLG
                     LAKRIQAKILQASTSEVYGDPKEHPQNESYWGNVNPIGLRACYDEGKRCAEALFMNYH
                     RQNQVKIKIIRIFNTYGPRMHPHDGRVVSNFILQALNNQDITIYGDGMQTRSFCYADD
                     LIDGMIRMMNTPEDFIGPVNIGNPVEFTILELAQLVIELTKSKSKIIFHPLPADDPMQ
                     RKPDIELAKTVLGWEPKIQLREGLEKTIAYFKK"
     gene            1715..2584
                     /gene="rfbD"
                     /locus_tag="PLI16_08900"
     CDS             1715..2584
                     /gene="rfbD"
                     /locus_tag="PLI16_08900"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOH84714.1"
                     /translation="MGNILVTGSNGQLGNEIRVLADTCTQHRYFFTDIDELDICNEKA
                     VDEFVSKNNITYIINCAAYTAVDKAEQEQQKAFEINATAVDNLCKAAKTAGAFMLQIS
                     TDYVFDGTKNTPYIETDKVNPVSVYGKSKLKGEEFVLSHGLPALIVRTSWLYSSFGNN
                     FVKTILRLAKEKAGLRIIADQYGTPTYARDLAKAVLQVVSDKTLPRSPEIYHFSNAGE
                     TNWYGFAKAITELSGITIPVEPIQTKDYPTAATRPPYSVFSKEKIIRDFNLHIPDWKD
                     SLKACLDILNKNN"
BASE COUNT          854 a          578 c          585 g          694 t
ORIGIN      
        1 agttgtataa gccctttacc ctgaatggcc atccggtaat atttatggac attcccagcg
       61 ccgaaatgac caaatatgcg gctaatgcca tgttggccac aaaaatcagt tttatgaacg
      121 atattgccaa cctgtgcgaa atcatgggag ccaatgtaaa catggtccgc aaaggaatag
      181 ggagcgacag ccgcattgga aacaaattta tttatccagg catcggttat ggcggatcat
      241 gtttccctaa agatgtcaaa gccttgataa aaaccgctga tgaaaacaag tacagcctta
      301 gggttttaaa agctgtggaa gaagtcaacg atgatcagaa aagcgtgctt tttaataaag
      361 ttatgaagta ttttaacggc cagcttaaag ataaaaccat agccatgtgg ggattgtctt
      421 ttaaacccga aaccgatgat atgcgggaag ctccggccct ggtgctcatc gaaaagtttt
      481 tacaggcagg tgccaaagtt aaagcctatg accccgaagc catgaaagaa gcaaaacgcc
      541 ggctgggcaa caaaattgaa tttgtcaatg atcagtacga agccctgatt gatgccgatt
      601 gcctgctgat agttaccgaa tggacagaat tcagagcacc caactttaat gtgataaaaa
      661 aattgttgaa aaaaaatgtg atcttcgatg gcaggaatat ctacgacctg gctgttatga
      721 aagccgaagg ctttgactat ttctgtatcg gaatcaatac cactaaataa ttatgagaaa
      781 aaagattttg gtaacgggcg gagccggatt cctgggatcg cacctgtgcg aaaggttact
      841 gaatgagggt aacgaagtga tttgtctgga caattatttc acaggacaaa aacaaaatat
      901 tgttcacctg ctcgataacc catactttga gttgatacga catgatgtga ccatgccatt
      961 ttttattgag gtggaccaga tatacaatct tgcatgtcct gcttcgccaa tacattatca
     1021 gtacaatcca ataaagacta tcaaaacttc cgtgatgggc gccattaaca tgctgggcct
     1081 ggccaaaagg atacaggcaa aaattttaca ggcctccacc agcgaagttt atggggaccc
     1141 caaagaacac ccgcaaaacg aatcgtactg gggcaatgtg aaccctatcg gtttgcgggc
     1201 ctgttacgac gaaggcaaac gctgcgccga agccctgttc atgaattatc accggcagaa
     1261 tcaggttaaa ataaaaatca tccgaatctt caatacctat gggccgcgca tgcaccccca
     1321 cgacggaagg gtggtgtcca actttatcct gcaggcgctc aacaaccagg acattaccat
     1381 ttacggcgac ggaatgcaga caaggagttt ttgttatgcg gacgacctta tcgatggcat
     1441 gatacgcatg atgaatactc cggaagattt tatcggccct gttaatattg ggaaccccgt
     1501 ggaatttacc attctggaat tggcacaact ggtgattgaa ttgacaaaat cgaaatcaaa
     1561 aatcattttc catcctttgc ctgccgatga ccccatgcaa cgcaaaccgg atattgaatt
     1621 ggcaaagact gtccttggat gggaacctaa aatacaactc agggaaggtc tggaaaagac
     1681 catcgcctat tttaaaaaat aacttgataa gataatgggt aacattcttg taaccggaag
     1741 taatggccag ctgggcaatg agatcagggt gctggctgac acctgtacgc agcaccggta
     1801 tttttttacc gatattgacg aactggatat ttgtaacgaa aaggccgtcg atgaatttgt
     1861 cagcaaaaat aatattacat acatcatcaa ttgtgctgcc tataccgcgg tggataaagc
     1921 agaacaggaa caacagaaag cttttgaaat taacgctact gccgttgaca atttgtgcaa
     1981 agctgccaaa acagccggtg cctttatgct gcaaatctcc acagattatg tgttcgatgg
     2041 cacaaaaaac acgccttaca tcgagacaga taaagtcaac cctgtttctg tttatggaaa
     2101 atcaaaacta aagggtgaag aatttgtttt atcccacggt ttgccggcac tgatagtgcg
     2161 tacctcatgg ctttattcat cctttggaaa caattttgta aaaaccatcc tccgcctggc
     2221 aaaggaaaag gccggcttgc gcattattgc cgaccagtac ggaacaccca cctatgcgcg
     2281 cgacctggca aaggcggtgc tgcaagtggt ttctgataaa actctgccgc ggagtcccga
     2341 gatttaccat ttttcaaatg caggagaaac caattggtat ggttttgcta aagctattac
     2401 tgaactttcg ggaataacta ttccggttga gccaatccag acaaaagatt accctaccgc
     2461 agcaacaagg ccaccataca gcgtgttcag caaagaaaaa attatccgcg attttaacct
     2521 gcatattcct gattggaaag acagcctcaa agcctgcctc gacatattga acaaaaataa
     2581 ttaaggcgac gcttaaatta ttttgccctt atcggagata ttgattaatt ttatttttcg
     2641 cacctatccc gatagtgaaa tatattttta gttaaaataa ttgttgtaat aacacctttt
     2701 tgttcaatat t
//
LOCUS       DAOSFP010000325         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_36080_length_2708_cov_3.334715, whole genome shotgun sequence.
ACCESSION   DAOSFP010000325 DAOSFP010000000
VERSION     DAOSFP010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36080_length_2708_cov_3.334715"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PLI16_08905"
     CDS             complement(<1..561)
                     /locus_tag="PLI16_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOH84715.1"
                     /translation="MMHKIFKPEIPIMLAFLLVFASSGVFSQNLDIDLLRKINLNRNR
                     KLDNTFRVITNSMRPVQIATPIVLFGLSLTTKDSVMRRNFYYVGVTILVTTAITSVLK
                     LSIRRPRPFVTYPDIERETDVGHLSFPSGHTSDAFALATSLSLAYPKWYVIVPMYLWA
                     GAVGYSRMHLGVHYPGDVLAGAVIGAG"
     gene            complement(662..817)
                     /locus_tag="PLI16_08910"
     CDS             complement(662..817)
                     /locus_tag="PLI16_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84716.1"
                     /translation="MNTSALLFMIITQAIITGLMLYFFFRVLFAKPKVGINEDSYADN
                     DEPAAKK"
     gene            complement(820..2634)
                     /locus_tag="PLI16_08915"
     CDS             complement(820..2634)
                     /locus_tag="PLI16_08915"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOH84717.1"
                     /translation="MVDNKLQWGSRVGLILAMAGNAVGLGNFLRFPVQAVQNGGGAFI
                     IPYLVCFLLMGVPLLFIEWSIGRFGGQFGHHTTPFAFARMSKARMWKYIGVFGIFSNI
                     TIASYYCYIESWTMSYVYHSAVGTFAGMDQNSVAAFFDDYLNVFTSTTGIPFEAIVFF
                     VVCLVLNTWVLSRGLSGGVEKAAKIGMPLLIVFGIFLAVKAIFLKAGQDGAVYDGTVG
                     LNFLWTPQFDSLANPKVWLAAAGQIFFTLSLGQGSVQCYAAYVKKKDDIALNAMSAGW
                     MNEFVEVVLGSAVIIPISIGYLGIDRVIELTSFGGLGLGFRTMPFLFQQWGPLLAAVA
                     GVAFFGLLFFAGITSTLAMGTPVMSFLIDEFNWRRSRAAMGFGFSVLLLGLPTVVFFK
                     KGVFDEYDYWAGTVSLVVFAMFEAILFAWVFGMKKGWQEITDGADIKVPAIFKYILKF
                     VTPVILILVFVTSLIRPANDDWSKVSITGWEVHPESIIAKIRNDHIGPNCEYFADTYY
                     AETAGVVDSVYTSEEKNVVAVVKDDQSIKTYETDMTNSVVVKKGDRVDAGVALFSGSF
                     INNVFYVDMSRLLLVALFLSICVLIFVAYRKRKKEGTI"
BASE COUNT          823 a          696 c          580 g          609 t
ORIGIN      
        1 tccggcccct atgacggcgc cggcaagcac atcgccggga taatgcacgc caagatgcat
       61 cctggagtag cccacagcac cggcccacag gtacataggc acaataacat accatttggg
      121 ataagccagg cttagcgagg tggccagggc aaaggcgtcg gaggtatggc ccgaaggaaa
      181 cgacaagtgc ccgacatcag tctccctttc aatatcggga taggtaacaa aaggccgggg
      241 ccgccggatg ctgagtttta aaacagaggt aatggccgta gtgaccagta tggtaacgcc
      301 cacataataa aaattccgcc tcatcacact gtctttggtg gtaaggctca gcccgaacaa
      361 cacaatgggc gtggctatct gcacaggccg catgctgttg gttatcaccc ggaaggtatt
      421 gtccagtttc cggttacgat tcaggttgat ttttcgtaac aagtcaatat cgaggttttg
      481 tgaaaagaca ccggaagaag caaacaccag caaaaatgcc agcattatcg gtatttccgg
      541 cttaaaaatc ttatgcatca taacctaaaa atttttcata tctgatagcc tcctgcccac
      601 tttaaaaaaa caatctgcac caacgtttac ctgcatcagt tgcctgccaa tcagcgggaa
      661 gttatttttt agcggccggt tcatcgttat cggcgtatga atcttcgttg atgcctactt
      721 tgggtttggc aaaaagcacc ctgaaaaaga aatataacat caggccggtg atgatggcct
      781 gcgtaatgat catgaacaat agtgcacttg tattcataat tatatcgtcc cttctttttt
      841 acgtttacga taggcaacaa atatcagcac gcagatggat aaaaacaaag ccacgagcag
      901 cagccgcgac atatccacat aaaacacatt attgataaaa ctcccgctga aaagagccac
      961 gcccgcatcc acccggtcgc cttttttaac tacgactgag tttgtcatgt ctgtttcgta
     1021 agttttgatg ctttgatcgt ctttaaccac agccaccaca tttttttctt cgcttgtata
     1081 aacagaatca accacaccgg ccgtttcggc ataatatgta tcggcaaagt attcacagtt
     1141 ggggccgata tgatcgttgc ggattttggc aataatactt tcggggtgca cctcccagcc
     1201 ggtgatgctc accttgctcc agtcatcatt ggccggacgg ataagcgagg tgacaaaaac
     1261 caaaatcagg attaccgggg taacaaactt caggatgtat ttaaagatgg cggggacttt
     1321 gatatcggcg ccgtcagtaa tctcctgcca accttttttc atgccaaaaa cccaggcaaa
     1381 aagtatggct tcaaacatgg caaaaaccac cagcgacacg gtgcctgccc aataatcata
     1441 ttcgtcgaac actcctttct taaaaaatac aacagtcgga agcccgagca acaataccga
     1501 gaacccaaag cccatggcgg cgcgcgaccg tcgccagttg aactcatcaa taagaaatga
     1561 cattacggga gttcccatgg ccaaggtaga ggtaatacct gcaaaaaaca gcaatccaaa
     1621 gaaggcgaca ccggccacgg cagccaacaa cggcccccac tgctggaaga ggaacggcat
     1681 ggtgcggaaa cccaggccca ggccgccaaa ggaggtaagt tcaattaccc tatcgatgcc
     1741 cagataacca atagatatcg ggatgatgac agcgctgccc agcacgactt caacaaattc
     1801 attcatccag cctgccgaca tggcgttgag cgcgatatcg tctttctttt tgacataagc
     1861 cgcataacac tgcaccgagc cttgccccag cgacagtgtg aaaaatattt gtccggctgc
     1921 tgccagccat actttaggat tagctaaaga gtcgaactga ggtgtccaga gaaaattcag
     1981 tcctacggta ccgtcgtaca cggctccgtc ctggccggcc ttcaggaaaa tagctttgac
     2041 ggccagaaaa ataccgaaaa ctatcaataa cggcatacct attttggccg ctttttccac
     2101 ccctccgctc aacccgcggc ttaacaccca ggtattcagc accaggcaca ctacaaagaa
     2161 aacgatggct tcgaacggta ttccggtggt ggaggtaaac acattaagat aatcatcaaa
     2221 aaatgcagcc acgctgtttt gatccatgcc ggcaaaagtc ccaacagcag aatgatatac
     2281 atatgacatg gtccagctct ctatatagca atagtacgac gcaatggtaa tatttgagaa
     2341 aataccaaac acgcctatat atttccacat cctggctttc gacatcctgg caaaggcgaa
     2401 aggcgtggtg tgatggccga actgtcctcc aaaacggcct atcgaccatt cgataaacaa
     2461 caaaggaaca cccatcagca gaaaacacac caggtaagga ataataaatg cgccaccccc
     2521 attttgcacc gcctgaaccg gaaatcgcaa aaaatttcca agacccaccg cgttgccggc
     2581 cattgccaga atgagtccta cacgcgagcc ccattgtaat ttattatcaa ccatagagtt
     2641 attcttgatt atacaattaa atatgttaag agtactacaa taataaaaaa taaaacaata
     2701 ataaaatc
//
LOCUS       DAOSFP010000326         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37081_length_2658_cov_3.736842, whole genome shotgun sequence.
ACCESSION   DAOSFP010000326 DAOSFP010000000
VERSION     DAOSFP010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37081_length_2658_cov_3.736842"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PLI16_08920"
     CDS             complement(<1..211)
                     /locus_tag="PLI16_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HOH84718.1"
                     /translation="MKKYNHLLFDLDKTIWDFAINSEDTIRELYGDFSLKHLGINDFI
                     TFFREYEKINLELWECYRQNEISKTD"
     gene            333..1214
                     /gene="dapA"
                     /locus_tag="PLI16_08925"
     CDS             333..1214
                     /gene="dapA"
                     /locus_tag="PLI16_08925"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOH84719.1"
                     /translation="MMIKGAYTLLITPFREDLTLDEEGLRVLVQRQVASGIQGIAPLG
                     VTGENTLMTNEEVYRVVEIVAQEAKGKAMIAPDICSTSLWEAKERLQRFVDLGADYIC
                     VFTPYFVLPKQDGLIEFYTKLADFSPVPILLHNAPERTGVDLLPETTGLLAKHPNIIG
                     IKDGNKKLDHLAKILHLTRQEDFCVFTGKDTTAYPLISFGGAGTFTVAGNVIPEVMKK
                     LTENALAGNLKEAAELHYSYYDLFEAFRYESNPMAAKMALNMMGLPAGGHRCPLTPLS
                     EEKTAKLKMFMQKKGLL"
     gene            1211..2131
                     /locus_tag="PLI16_08930"
     CDS             1211..2131
                     /locus_tag="PLI16_08930"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOH84720.1"
                     /translation="MKKIRLRGPATTANVGPGYDIFAMALKEPHDEVTLELTDDQQIT
                     IAVENNPQQIVPLNPEKNTAGLAILEIKKRYHLDFGVKVTISKLMTSGGGLGTTGASS
                     AAAVYGMNKLLNLNLSPNEMVDIARFGEVASGGSPHADNVAAALLGGFVLVKNYNPLE
                     VVKLEMPEFPVVLAVIQKKQGTTRGLITYEIGQEKLKDQMAHCCSVIHSIMTKDLKEF
                     GKAISVDHIAEPVRATTIMNYYETREAILKAGAYGTNVSGGGSSVFAVCEKSLQDKIA
                     EIMEKGLGQNPNFDRVIKTTTSNTGVTEIA"
     gene            2128..>2658
                     /locus_tag="PLI16_08935"
     CDS             2128..>2658
                     /locus_tag="PLI16_08935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH84721.1"
                     /translation="MKIDFSGKTVLVTGASRGIGRATAVRFAECGATVIVHYKDFGGG
                     ALKTLSLMAEGKHFLMKADLTDPAQVENLVTKSFRKAGKIDVLVNNAGIYRECDLREC
                     SFTEWQKNFDDTFRTNLYAPAYLSYFVARHMAAHGGGKIINVSSRGAFRGEPMAPAYG
                     ASKAALNQFSQSLAYLM"
BASE COUNT          786 a          633 c          616 g          623 t
ORIGIN      
        1 gatcagtctt gctaatctcg ttttggcggt aacattccca caattccaga tttatctttt
       61 catattcccg gaaaaaagtt atgaaatcgt tgatgcccag atgcttcaag ctaaaatcgc
      121 cgtacaactc ccggatggta tcttcagaat ttattgcaaa atcccaaatg gttttatcca
      181 gatcaaacag taaatggttg tattttttca taattgaacg gcaaaaataa tcattgtttg
      241 ttaaaaagaa tgtgagaaaa aaaatctccg gggtttttaa gaacgttcat tttcgctatg
      301 tttgcaaaat aatattatta aaacctaatt ttatgatgat taaaggcgca tatacattat
      361 tgatcacccc gttcagggaa gatctcacac tggatgaaga aggtctaaga gtacttgttc
      421 agcggcaggt agcctcgggc atacagggca tcgctcccct gggtgttacc ggcgaaaaca
      481 ccctgatgac caacgaagaa gtatatcgtg tggtagaaat tgttgcacaa gaggctaaag
      541 gaaaagccat gatagcccct gacatatgca gcaccagttt atgggaggcc aaagaacgcc
      601 tgcaacgttt tgttgatctt ggcgctgatt atatctgtgt cttcacacca tattttgtat
      661 tacccaaaca ggatggcctc atagagtttt ataccaaact ggctgatttc tcgccggttc
      721 ccatcctgct ccacaatgca ccggaaagaa ccggagtgga cctgctgccc gaaaccacgg
      781 gattgctggc aaaacatcca aacatcatcg gtatcaagga tggtaacaaa aagctcgacc
      841 acctggccaa gatcctgcac ctcacccggc aggaagattt ctgtgtgttt accggaaaag
      901 acactacagc ctatccgttg atttcttttg gcggtgctgg aacctttacc gtggccggca
      961 acgtgattcc cgaagtgatg aaaaagttga ccgaaaatgc cctggcagga aacctgaaag
     1021 aagcggctga actacactac agctactacg acctgtttga agccttccgc tatgaatcca
     1081 acccgatggc ggccaaaatg gccctcaata tgatgggact tcctgccgga ggacaccgtt
     1141 gccccctcac ccccctgagc gaagaaaaaa cggccaaact taaaatgttt atgcagaaaa
     1201 aaggtttgct atgaaaaaaa tcaggttgag aggtcctgca accacagcca acgttggtcc
     1261 gggctacgat atctttgcta tggccctgaa ggagccccat gatgaagtta cactcgaact
     1321 tactgatgat caacaaatta ctattgccgt agaaaacaac ccacagcaga tagttccctt
     1381 aaaccccgaa aaaaacaccg ccggactggc catcctcgaa ataaaaaaaa gataccatct
     1441 tgatttcggt gtaaaagtaa ccatatcaaa acttatgacc tcgggcggag gcctgggcac
     1501 caccggcgca tcgtcggcag cagctgttta cggaatgaac aagctgctga acctgaactt
     1561 atccccgaac gaaatggtgg acattgcccg ttttggcgaa gtggcttccg gaggctcgcc
     1621 ccatgcagac aacgtggctg cggcgctgct cggaggattt gtgctggtaa aaaattacaa
     1681 cccgctcgaa gtagtaaaac ttgaaatgcc cgagtttcct gtggtactgg cagtaataca
     1741 aaaaaagcaa ggtacaacgc gtggtttaat cacctatgaa atcggtcagg aaaagctcaa
     1801 agaccagatg gcacactgtt gcagcgttat tcacagcata atgaccaaag accttaaaga
     1861 attcggcaag gcaatttcgg tggatcatat tgcagaaccc gtgcgcgcaa ccactattat
     1921 gaattactat gaaaccaggg aagccattct taaagccggc gcttatggca ccaatgtcag
     1981 tggaggcggc tcttcggtat ttgctgtctg cgagaaatca ttacaagaca aaattgccga
     2041 aataatggaa aaaggtctgg gacaaaatcc caatttcgac agggtgataa aaaccactac
     2101 cagtaataca ggtgttacgg aaatagcatg aaaattgatt tcagcggtaa aacggtattg
     2161 gttacaggcg cctcgcgagg cattggcagg gccaccgcgg taagatttgc cgaatgcggc
     2221 gccactgtaa ttgtacatta taaagacttc ggcggcggcg cgctgaaaac gctgtcgctc
     2281 atggccgaag gcaaacactt tttgatgaaa gccgacctta ccgatccggc gcaggtcgaa
     2341 aacctcgtta ccaaatcctt cagaaaggcc gggaaaattg atgtacttgt caacaatgca
     2401 ggtatttaca gggagtgcga cctccgggaa tgttccttta ccgaatggca aaaaaacttc
     2461 gacgatactt tccgcacaaa cttgtacgca ccggcatacc tgtcgtattt tgtcgccaga
     2521 cacatggccg cacacggcgg aggcaaaatt atcaatgtat cttcgcgggg cgcgttcagg
     2581 ggagagccta tggctcctgc ctacggtgcc agcaaggcag cgctcaatca gttcagccag
     2641 tcgctggctt acctgatg
//
LOCUS       DAOSFP010000327         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37166_length_2654_cov_3.615621, whole genome shotgun sequence.
ACCESSION   DAOSFP010000327 DAOSFP010000000
VERSION     DAOSFP010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37166_length_2654_cov_3.615621"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="PLI16_08940"
     CDS             <1..400
                     /locus_tag="PLI16_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84722.1"
                     /translation="DGNGTGSFSSNLTSLSPNTTYYIRAYATNCVGTGYGNQTTFKTQ
                     TITEINENKTWYNSVKVYPNPVSDELIIETNGDESIINVEILNSFGAIIFKGDLFQTT
                     AIKTDGFASGVYMLKLSDGKSLEVRKIIKE"
     gene            complement(409..1575)
                     /locus_tag="PLI16_08945"
     CDS             complement(409..1575)
                     /locus_tag="PLI16_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84723.1"
                     /translation="MKTKVVFFLISVVLLAAGCTSSKQYLEKGNFDMATKKAIAKLRK
                     NPDNVKQLAVLTQAYKQARRQNLDRIDYLRKTGQPDIWEEVFQNYNALKNRQDMAKSL
                     PQHILASIGFEYHDYDQDIIEAEKKACDYYYALGNRLLKSNDKMSARQAYSEFTKIKK
                     HYSDYKDVDELIRQAEIKGTTFVILYMQNNSNAILPKDFEYEFLKISLTGLNKKWVQY
                     DTREQEGLAYDYAIVVNLKMIDVSPERIKEKIWTEAKTIQDGWTYQLDNRGNVMKDSL
                     GNDIKVPKYVRTECHVKEIAQNKEAILSGKVDFYNNVSKQLMTSENIIANSIFSNGYL
                     IANGDLRALSPETQKRLGSVPLPFPSDFDMILMANEFLQKAVMDVVNNKSYLIK"
     gene            1783..>2654
                     /gene="rfbA"
                     /locus_tag="PLI16_08950"
     CDS             1783..>2654
                     /gene="rfbA"
                     /locus_tag="PLI16_08950"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOH84724.1"
                     /translation="MKGIILAGGSGTRLYPLTIAVSKQMMPVYDKPMIYYPLSVLMMA
                     GISEVLIISTPYDLPNFQKLLGDGSELGCKFSYAPQEIPNGLAQAFVIGEKFIGNDKV
                     ALVLGDNIFYGTGLPKLLQENNDPDGGVVFAYHVSDPERYGVVEFDEKLQAISIEEKP
                     QAPKSNFAVPGLYLYDNSVVEVAKNIKPSKRGEYEITDVNKYYLEQKKLKVAILTRGT
                     AWLDTGTFSSLLQASQFVEVIENRQGLKIGCIEEIAYRMGFIDAEKLRFLGQKYIKSG
                     YGEYLLKILEQKQL"
BASE COUNT          715 a          525 c          599 g          815 t
ORIGIN      
        1 cgatggtaat gggacaggtt ctttttccag caatcttaca tcgttgtcac ccaacactac
       61 ttattacata cgtgcttatg ctacaaattg cgtaggtaca ggatatggaa atcaaaccac
      121 ctttaaaacg caaaccatta ctgaaataaa tgaaaataaa acctggtata atagcgtaaa
      181 agtttatcca aatcctgttt cagatgaatt gatcatcgaa actaatggtg atgaaagtat
      241 catcaatgtt gagattttaa actcatttgg agcaataatt tttaaaggcg acttatttca
      301 aacaaccgct atcaaaacag atggttttgc ctccggcgta tatatgctaa aactaagcga
      361 tggaaaatcg cttgaagtta gaaagattat caaagagtaa aacgcaagct actttatcag
      421 atacgacttg ttgttgacca catccatcac ggctttttgc agaaattcgt tggccatcag
      481 tatcatatca aagtcgctgg ggaagggcag gggtacgctg cccagacgtt tctgggtttc
      541 gggcgataat gcccgcaggt cgccgttggc aatcagatag ccattgctga atatggaatt
      601 ggcgatgatg ttttcggaag tcatcagttg tttgctgaca ttgttgtaaa aatccacctt
      661 gcccgagagt atggcttctt tgttttgtgc tatctccttc acatgacatt cagtcctcac
      721 atatttaggc accttaatat cgttgcctaa gctgtctttc atcacattgc cgcggttgtc
      781 gagctggtag gtccagccgt cctgtatggt tttggcttcg gtccatattt tttcttttat
      841 gcgttcgggc gagacatcaa tcatcttcag atttactact atggcgtagt cgtatgccaa
      901 gccttcctgt tcacgggtat cgtattgcac ccattttttg ttgaggccgg taagcgatat
      961 cttcaaaaat tcatactcga aatccttggg caaaatggcg ttgctgttgt tttgcatata
     1021 cagtatcaca aaggtggtgc ctttaatctc ggcctgcctg ataagctcgt ccacatcctt
     1081 gtaatccgaa taatgttttt tgattttggt aaactcgctg tatgcctggc gtgccgacat
     1141 tttgtcgttg ctcttaagca gcctgttgcc gagtgcgtag taatagtcac aagctttttt
     1201 ttcggcttct attatatcct ggtcatagtc gtgatattca aatccgatag aggcaagaat
     1261 gtgttgcggc aggcttttgg ccatgtcctg gcggtttttt agcgcattgt agttttggaa
     1321 gacttcttcc cagatatcgg gttggccggt tttgcgcaaa taatctatcc tgtcgaggtt
     1381 ttgccttctg gcttgtttat aggcctgggt aagcaccgcc aactgtttta cattgtcggg
     1441 atttttacgc agtttggcga tggctttttt tgtagccata tcaaaattgc ctttttcaag
     1501 gtattgtttt gaagaggtgc atcctgcggc taggagaacg acgcttatca ggaaaaaaac
     1561 aacttttgtt ttcataccgg ttcttttttt aaaaaataca aaatttaagc caaaaatcag
     1621 ctcgaccaaa gataggataa ttcttgtatt ttatgcacct gcttatacgg ttatgctcgc
     1681 tttcggagag aaataaattg caatgtcaat atcaggaatt gttgtacttt tacttttttt
     1741 tatgctaatt ttgaaacata atcgtaataa aaaagccaaa agatgaaagg tataatcctt
     1801 gccggaggtt ccggcacccg gctgtatccg ttgaccattg ccgtgagcaa gcagatgatg
     1861 cctgtttatg ataagcccat gatttattac ccgttgagtg tgctgatgat ggcgggaatt
     1921 tcggaggtgc tgataatttc tacgccttac gacctgccga attttcaaaa acttttaggc
     1981 gacggcagcg aactgggatg caaattcagc tatgctcccc aggaaatccc caatggactg
     2041 gcacaggctt ttgttattgg agaaaagttt attggcaacg acaaggtggc gctggtactt
     2101 ggcgataata ttttttatgg caccggcctg ccaaagcttt tgcaggaaaa caacgatccc
     2161 gatggcgggg tggtgtttgc ctatcatgtt tctgatccgg agcgttacgg tgtggtggaa
     2221 tttgatgaaa aactgcaggc aatatccatc gaagagaagc cgcaggcgcc caagtcgaat
     2281 tttgcggttc ccggcttgta tctttacgat aacagtgtcg tggaggtggc aaaaaatatc
     2341 aaacccagca aacggggcga atacgaaatt accgacgtaa acaaatatta tctggaacag
     2401 aaaaaattga aagtggccat acttacccgc ggaactgcct ggctagacac cggcactttc
     2461 tcatctttgc tgcaggcatc gcagtttgtg gaggttattg aaaacaggca gggcctgaaa
     2521 attggctgta tcgaagagat agcctatcgc atgggcttta ttgatgctga aaaattgagg
     2581 tttttgggcc agaaatatat aaaatccggt tatggagaat atcttttaaa aatattagaa
     2641 caaaaacaat tatg
//
LOCUS       DAOSFP010000328         2645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37342_length_2645_cov_4.434749, whole genome shotgun sequence.
ACCESSION   DAOSFP010000328 DAOSFP010000000
VERSION     DAOSFP010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37342_length_2645_cov_4.434749"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLI16_08955"
     CDS             <1..212
                     /locus_tag="PLI16_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84725.1"
                     /translation="LKVAAINCGDLTIFETLNILISNTINLQIFYYRFSQEGFAHVLI
                     GKEPSLPQAHNFTVQGNIDLIQSMQ"
     gene            complement(387..968)
                     /locus_tag="PLI16_08960"
     CDS             complement(387..968)
                     /locus_tag="PLI16_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84726.1"
                     /translation="MKTKVVFFLAVLFLVSCAQINQLTNLLTCDFRLSTAENITLGGV
                     NVQKLKSFSDLKLVDGAALLANVASGSLPLHFTLNVEARNPNKEKAALNKLDWIALID
                     DTQIATGTTAKRVEIAPNNGTGTFPIQVNTDLVKLLSGQAGKNIINFGLNLVDAGNRP
                     TRISLKAKPTVMVGSYALQYPGYITIKKDFGAK"
     gene            complement(1085..1405)
                     /locus_tag="PLI16_08965"
     CDS             complement(1085..1405)
                     /locus_tag="PLI16_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84727.1"
                     /translation="MKLKNFNWSVFLLTTIILCVLLIPSSFAALAEDEGTLSADDTFM
                     SFFVGLFYILRFPTHTLVLTSLSLGGPLVFWGGLLVNCMIYGLLTERIVSVFRGIFWK
                     NKSG"
     gene            complement(1448..1870)
                     /locus_tag="PLI16_08970"
     CDS             complement(1448..1870)
                     /locus_tag="PLI16_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84728.1"
                     /translation="MKKVSLELSPEQYKELLKLVYVGDWVIDETENIVLNDMVQTVFS
                     KAEEAGLKGLVEFNKEQNLFVPSLQMDEEVIDLINDYDDECFWDTLIFGLAERDAEKV
                     LGEDLENKSLEEKVELLEPYTKKYIKEFEENGLDNIKV"
     gene            complement(1881..>2645)
                     /locus_tag="PLI16_08975"
     CDS             complement(1881..>2645)
                     /locus_tag="PLI16_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009010756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOH84729.1"
                     /translation="SVGVSQDELIKIAIKSLGLDDLKPFNPREKIIEFVLEDKKAVKT
                     RKLIDMSCAAFADETASESPAPGGGSISAYMGALGASLATMVANLSSHKPGWDERWEE
                     FSNWAEKGQKIKDELLALVDEDTNAFNKIMNAFGLPKGNDQEKAIRTQAIQDATRYAI
                     EVPYKTMEKALASFEIIKAMAEVGNPNSVTDAGVGALCARSAVMGAFLNVKINACGLK
                     DKDFVANTIAKGEEMLKKAIAEEESVLKIVHENINF"
BASE COUNT          713 a          594 c          542 g          796 t
ORIGIN      
        1 aactaaaagt agccgcaata aattgcgggg atttaaccat ttttgaaaca ttaaacattt
       61 taatatcgaa tacaattaat ttacaaatat tttattacag gttttctcaa gaaggctttg
      121 cgcatgtact tattgggaaa gagccatctc tcccacaggc acataacttc actgtgcagg
      181 gtaatattga tttaatccaa agtatgcaat aacggaattt gcctatgggt gccggagaca
      241 gaagttgggt gatttaatac tgagtggatg aatgcgcaca agaaggagct tttgccagtc
      301 atccgcacag tggtgtccgg tatgcaggct tataaacaag aaatttcaat ccgccttacc
      361 gcactgattg atggttcatt ttggttttat ttagctccaa aatccttttt gatcgtaata
      421 taaccgggat attgcagggc atagcttccg accatgacag taggtttggc tttaagtgag
      481 atacgcgtag gccggttgcc agcatccacc aggttaaggc caaaattgat gatatttttt
      541 ccggcctgcc ctgaaagcag tttcaccagg tcggtattta cctgtatcgg gaaagtgcct
      601 gtgccgttgt ttggtgctat ctccaccctt ttggctgtgg tgccagtggc tatctgggta
      661 tcgtcaatta acgcaatcca gtcgagttta ttcagcgctg ctttttcttt gttggggttg
      721 cgggcttcca cgttcagcgt aaagtgcagc ggtaaggagc cggaagccac attggccagc
      781 aaagcagcgc catcaaccag cttcaggtcg ctgaaagatt ttagtttctg aacattgacg
      841 ccgcccagcg tgatgttttc ggctgtagac aagcgaaaat cgcaggtcag cagattggtc
      901 aactgattta tctgagcaca ggataccagg aatagaactg ccaaaaagaa cactactttt
      961 gttttcatag ctttgaattt ttttacaata tgtttttgtt tcggtatcaa atcactgtta
     1021 aggtaaaata tacaaaacag ctttgtattc tatttcaaaa accacgccgg aatacacctc
     1081 ccagctatcc cgatttgttt ttccaaaata ttccacgaaa aactgaaaca atcctttctg
     1141 tcaatagtcc gtaaatcata caattgacta aaagtccacc ccaaaaaacc agcggtccgc
     1201 caagcgaaag acttgtcaaa accagggtat gagtcggaaa gcgaagaata taaaaaagcc
     1261 caacaaaaaa actcataaaa gtgtcatctg cgcttaaagt tccttcatct tcggccaatg
     1321 cagcaaatga tgaggggatt aacagcacac ataatatgat agtcgtaaga agaaaaacgc
     1381 tccagttaaa atttttcaat ttcataaaat ggcgttttac atccaaagat attatgtgct
     1441 gttccgctta tacctttata ttatccaggc cgttctcttc aaattccttg atatactttt
     1501 tggtgtaggg ctccaatagt tccacttttt cttccagaga cttattttcc aaatcttcgc
     1561 caagcacttt ttcggcatcc ctttcggcaa gcccgaatat cagggtatcc caaaaacact
     1621 cgtcatcata atcgttgatg aggtcaataa cttcttcatc catttgcaac gaaggcacaa
     1681 agaggttttg ttctttgttg aattccacca gccctttcag accggcttct tcagcttttg
     1741 aaaaaactgt ttgaaccata tcgttcagta caatattttc ggtttcatca atcacccagt
     1801 cgcccacata tacaagttta agaagttctt tgtattgttc aggtgataat tcaagactaa
     1861 cttttttcat ggtattttat ttagaaatta atattttcat gtactatttt taacacggat
     1921 tcctcttcgg caatggcttt tttgagcatt tcctcacctt tggcgatggt attggctaca
     1981 aagtctttgt ctttcagtcc gcaggcattg atcttaacgt tgagaaaagc gcccatcaca
     2041 gccgatctgg cacacagcgc tcccacaccg gcatcggtaa ctgaattggg gttacccacc
     2101 tcggccatag ccttgatgat ttcaaaagaa gccagggctt tttccatggt tttatagggt
     2161 acttcgatgg catagcgtgt agcatcctgt atggcttggg tgcggatggc tttttcctgg
     2221 tcgttgccct ttggcaggcc gaaagcattc attattttgt tgaatgcatt ggtatcctca
     2281 tccaccagcg ccagcagttc gtccttgatc ttctgacctt tttcggccca gttagaaaat
     2341 tcttcccaac gttcgtccca tccgggttta tgtgatgaca ggttggccac catggttgcc
     2401 agcgaagcgc ccagagcgcc catatatgcc gaaatggaac ctccaccggg ggccggcgat
     2461 tccgaagccg tttcatcagc aaaagcagca cagctcatgt ctatcagctt gcgggttttg
     2521 acggcctttt tatcttcgag gacaaattca atgatctttt cacgcgggtt aaaaggtttc
     2581 agatcgtcga ggcccagcga ttttatggca atttttatca gttcatcctg tgaaacacct
     2641 accga
//
LOCUS       DAOSFP010000329         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37690_length_2629_cov_4.059441, whole genome shotgun sequence.
ACCESSION   DAOSFP010000329 DAOSFP010000000
VERSION     DAOSFP010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37690_length_2629_cov_4.059441"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PLI16_08980"
     CDS             <1..352
                     /locus_tag="PLI16_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84730.1"
                     /translation="FNSTVKVLTILWSVNHKEIKNQLLTKMAQVQKFIQERNINCTAE
                     MIEADSESDMVPSLLVYAKQQGDIDLILIMTQQETGLIPFFVNSHASDIIRRSDFPVM
                     SIVPKDMGETLSFK"
     gene            349..1581
                     /locus_tag="PLI16_08985"
     CDS             349..1581
                     /locus_tag="PLI16_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOH84731.1"
                     /translation="MTASVISIGDELLIGQTVNTNASFIAAELNLAGIQVRSILTIAD
                     DKDDIRNALQQASRQSGIVIITGGLGPTNDDITKNTLCEYFGSTLILHKPSLENIRKL
                     FYQRGMKITTVNRNQAMVPHNCTVIKNTLGTAPGMWFRKDNVHYFSLPGVPFEMKALI
                     TGGVIPLLAKLNISSPIIHKTILTIGIGESALAELISGWENKLPDHIKLAYLPEPGIV
                     KLRLSCYQAGSGETKKTFEAEVKKLKKLIPGLIYGYEDDTLAGVTGKMLLKKQASIAT
                     AESCTGGLIAHLITAVPGASEYYKGSVIAYDNDIKSKVLGVNKKLIEKYGAVSEEVVI
                     AMAQNVKKIMATDYALAVSGIAGPAGGTAEKPVGTVWVACATPEKTLTQKLSLGNNRE
                     RNIKRASLAALNLLRKQL"
     gene            1646..>2629
                     /locus_tag="PLI16_08990"
     CDS             1646..>2629
                     /locus_tag="PLI16_08990"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOH84732.1"
                     /translation="MSLEKFTGHLQNEYAFSGKSIILGGAMYEKKSVPGLLIRLPLAT
                     VNRHGLIAGATGTGKTKSIQRITESLSEQGVNVLLMDIKGDISGISKAGAENPKIKER
                     MDMIGLPWQPCEYPTELMTISHEKGLRLRATVSEFGPVLFSKILELNENQEGTVAIVF
                     KYCDDHQLPLLDIKDFRAALNYVATGDGKKEIAENYGLISPASAGVIMRKLLELEQQG
                     GDLFFGEKSFEVEDLLQKTSDGKAYINILRLTDIQDRPKLFSTFMLCLLAEIYHKFPE
                     IGDKGEPKLIIFIDEAHLIFKEASNALMDQIETIIKLIRSKGVGIYFCAQAP"
BASE COUNT          806 a          628 c          592 g          603 t
ORIGIN      
        1 gtttaactct accgtgaaag ttctcaccat tttatggtcg gtaaaccaca aagagataaa
       61 aaaccagctt ttgacaaaaa tggcccaggt gcagaagttt attcaggaac gcaatataaa
      121 ttgcacggcc gagatgattg aagctgacag cgaatcggat atggtgccat cgctgcttgt
      181 atatgccaaa caacagggcg atattgacct tatcctcatc atgacacagc aagaaacagg
      241 actgataccc ttctttgtca actcccatgc ttcggacatc atccgcaggt cggattttcc
      301 ggtcatgtca atagttccca aggacatggg tgaaacgcta tcctttaaat gacagcatct
      361 gttatcagta taggcgacga acttctgata ggacaaactg tcaatacgaa tgcttctttt
      421 attgctgctg aactgaacct ggcgggtatt caggtaagaa gcattctgac catcgccgac
      481 gacaaagacg atatcagaaa tgcgttgcaa caggcttcgc ggcagtcggg tatcgttatc
      541 atcacaggcg gcctggggcc aaccaacgat gacattacaa aaaacactct ttgcgaatat
      601 tttgggtcaa cactgatttt acacaagccc tcactggaaa atatccgcaa attgttttat
      661 cagcgcggta tgaaaattac caccgtcaat cgcaaccaag ctatggtacc ccataactgc
      721 acggtaataa aaaacacgtt gggtaccgca ccgggcatgt ggtttcggaa agacaacgta
      781 cactactttt cgttgcccgg cgtacctttt gaaatgaaag ccctgattac cggcggtgta
      841 atccctttgc tggccaaact caatatttca tcacctatca tccataagac catcctcacc
      901 ataggcattg gcgaatcggc tctggccgaa cttatcagcg ggtgggaaaa caagcttccc
      961 gaccatatca aattagccta tctgcccgaa ccgggtattg taaaactcag gctgtcgtgc
     1021 taccaggccg ggtctggtga aacgaaaaaa acttttgagg cggaagtaaa aaaattaaaa
     1081 aagctaatcc ccgggcttat ctatggatat gaggacgaca ccctggcagg agtaacaggc
     1141 aaaatgctgc tgaaaaaaca agccagtatt gccaccgccg aaagttgcac aggcggactc
     1201 atagcacacc tgattactgc agtgcccggg gcgtcagaat attataaggg atcggttatc
     1261 gcttacgata atgatataaa atcaaaagtg ctcggggtaa acaaaaaact tattgagaaa
     1321 tatggagctg tgagtgaaga ggtggttatt gccatggcac aaaacgtaaa aaaaattatg
     1381 gcaacagatt acgccctcgc ggtttcgggt attgccgggc cggcaggcgg cactgccgaa
     1441 aaaccggtag gaacagtttg ggtagcctgt gccacccccg aaaaaaccct tacccaaaaa
     1501 ctttcgctcg gcaacaaccg cgagcgcaat attaaacggg ccagccttgc cgcccttaac
     1561 ctgctgagaa aacaactctg accctgtcgg aaaaaaattt tcttatcttt gaccaaagaa
     1621 ataattcaat aaaaagtatt ttgtcatgtc gctggaaaaa ttcaccgggc atctgcaaaa
     1681 cgaatatgct ttttcaggta aaagtattat ccttggcgga gccatgtatg aaaaaaaatc
     1741 tgtccccggg ctgctgataa ggctgccgct ggccaccgtc aaccggcatg gccttattgc
     1801 cggagctacc ggtaccggaa aaaccaaatc catacaacgc atcaccgaat ctctttcaga
     1861 acagggcgtc aacgtgttgc tgatggatat caagggcgat atcagcggga tttcaaaagc
     1921 aggtgcagaa aaccccaaaa tcaaagaacg gatggacatg ataggcctgc cgtggcaacc
     1981 ctgcgagtac cccactgaac tgatgactat ctcgcacgaa aaaggattac ggctccgtgc
     2041 aaccgtttcg gaattcgggc ccgtactttt ttccaagata ctggaattga acgaaaacca
     2101 ggaaggaacg gtggccattg tattcaaata ttgcgacgac caccaactgc cgcttttaga
     2161 cataaaagac tttcgcgccg cactgaatta tgttgctacc ggcgacggca aaaaagaaat
     2221 agccgaaaat tacggcctga tttcaccagc ctcagcaggg gtaataatgc gcaaactgct
     2281 ggaactggaa caacaaggtg gagacctgtt ttttggggaa aaatcgtttg aagtagaaga
     2341 cctacttcag aaaaccagcg acggtaaggc atacatcaac atcttgagac tcaccgatat
     2401 tcaggacagg cctaaacttt tttcgacctt tatgctttgt ctgctggcag agatatacca
     2461 caaattccca gaaattggtg ataaaggaga gcccaaactt atcattttca ttgatgaggc
     2521 ccacctgata tttaaagagg cctccaacgc gctgatggac cagattgaaa ccattataaa
     2581 actcatccgc agcaaaggcg taggtattta tttctgcgca caggcgccc
//
LOCUS       DAOSFP010000330         2628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37716_length_2628_cov_3.146133, whole genome shotgun sequence.
ACCESSION   DAOSFP010000330 DAOSFP010000000
VERSION     DAOSFP010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37716_length_2628_cov_3.146133"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLI16_08995"
     CDS             <1..364
                     /locus_tag="PLI16_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84733.1"
                     /translation="GNFRTEYYYDEKGIREKTLKYNAKGQLVEKNLFTTDDQDNVIEI
                     YEEDTRSIKTTRFTYDESNRITRQEEYNRDEDLLMSIERTYNEDGKLAEALVYTQNPA
                     ENIRSMSATKYEYFYYDE"
     gene            467..1939
                     /locus_tag="PLI16_09000"
     CDS             467..1939
                     /locus_tag="PLI16_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOH84734.1"
                     /translation="MSLKLSKTDLLFLASGLLIFLYIVVKASISSFTTDESFSYLHYA
                     HTSFLDIISFSDWYTNNHILNSLLMKYAEVLFGSSEISLRLPNLLLFAVYMVYAFLLF
                     RNSDRLVALAVFILLCTNPGMVDLFGLARGYGLSYGFLLMSLYHFIRYFQTRKSKDMV
                     LFHIASMLAVLSSFTMLISYAALMLIYFITDFINSRVILKKKYLFFESVKVHIIPLVL
                     ITIILYEPVRRVFMRSELNFGGKEGFYGNTVSDLIKNIFHGVEISPFWMIMAQIVITA
                     LVLAGSVIILRLFFKKDGPSFFQFIGLFITNLLVIFISLAIVFQHLILGTDYPIARFS
                     LFLFPVFIVYFGFLLSYISQKNKNLVAVSGLCLALLSAGSFVYKADLRSCSEWRFDME
                     TKNMMRMLSDEHAANEAETAEVRLGIHWLFEPTVNFYRETWQLDWLLPVDRRGFTAED
                     DYIYTFRDEFIGFPNDKYEVVHEFETANTVLMKRLEKTSQ"
     gene            complement(1955..>2628)
                     /locus_tag="PLI16_09005"
     CDS             complement(1955..>2628)
                     /locus_tag="PLI16_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84735.1"
                     /translation="ALGLIMLVRPVNGIILLALFFVPTGWQGFKKGLSELFLHPWYPL
                     AGLAIFAAIISIQPILYKISTGSFFTDSYGSEFFNFSNPHLFDFLFSYKKGAFLYTPI
                     LFLSLTGFYFFWKTDRYIFYGLLFFVLVLFYVLSSWWNWWYGGSFSSRVLIEFLPVFM
                     LLLAKAIDGFRNKAVKGIFVGLIAICVLVNQIQIYQYRYGMIHFEQMDKQKYWENFLR
                     IDRLL"
BASE COUNT          805 a          515 c          522 g          786 t
ORIGIN      
        1 tggcaatttc aggacggagt attattatga tgaaaaaggc atacgtgaaa aaacattgaa
       61 atacaatgcg aagggacaac ttgtagagaa aaatcttttt accaccgacg accaggataa
      121 tgttattgaa atctacgaag aagatacccg ttcgataaaa acaacccggt ttacctatga
      181 cgaaagcaac aggattacca ggcaggaaga atacaacaga gacgaagacc tcctgatgag
      241 tattgaacgc acatacaatg aagatggaaa actagccgaa gccctggtat atactcaaaa
      301 tcctgccgaa aacatccgca gtatgtctgc cacaaagtac gaatacttct attatgacga
      361 ataaggaatg aaatccggaa cataatcatc ccgcacaaaa attccaacta agcccatcat
      421 aatctagagg cttacaagaa accaaaagca tatatattta taccagatga gtctgaaact
      481 ttcaaaaact gatttattgt ttcttgcttc gggcctgctg attttcttgt atatagtcgt
      541 taaagcttcg atatcatctt ttactaccga cgagagtttt tcatacttac actatgctca
      601 taccagtttt ttggatatca tttcctttag cgactggtac accaataatc atattttgaa
      661 cagcctgctc atgaagtatg cggaagtgct tttcggctcg tccgaaattt ctttaagact
      721 gccaaatttg ctgttgtttg cggtgtatat ggtgtatgct ttcttattgt tcagaaacag
      781 cgacaggcta gtagctttgg ctgtctttat tcttttatgt actaaccccg gaatggttga
      841 tttatttgga ttggccaggg gatatggctt gtcgtatgga tttttgctga tgagtttata
      901 tcattttata aggtattttc agactagaaa atcaaaagac atggtgcttt ttcatatcgc
      961 atccatgttg gccgtgctga gcagtttcac tatgctgatt agttatgcgg cattgatgct
     1021 gatctacttt attaccgatt ttattaatag ccgggtaatt ttgaaaaaga aatacttgtt
     1081 tttcgaatca gtcaaagtcc atattatacc ccttgtgttg attaccatca ttctgtacga
     1141 gccggtgcga agggttttta tgagaagcga gttgaacttt ggcggcaaag agggctttta
     1201 tggtaatacg gtcagcgatt taatcaaaaa tatattccat ggcgttgaaa tatcgccttt
     1261 ttggatgata atggcgcaaa tagtaatcac agcactggtt ttggccggga gtgtaattat
     1321 tttgaggctg tttttcaaaa aagatggtcc ttcctttttt cagtttatcg gcctgtttat
     1381 aaccaacctg ctggtcatct ttatttctct ggctattgtt tttcagcatt tgattcttgg
     1441 aacagattat cctatcgcgc gtttttcact ttttcttttt cctgttttca ttgtctattt
     1501 tggatttttg ctttcgtaca tcagccaaaa aaataaaaac cttgtggccg tttcgggatt
     1561 atgccttgcc ctgttatctg ccggaagctt tgtttataag gccgatctgc gttcctgttc
     1621 ggaatggcgt ttcgatatgg aaaccaaaaa catgatgcgc atgctgtctg atgagcatgc
     1681 cgccaatgag gcagaaactg cagaggtacg cctgggaatt cactggctct ttgaacccac
     1741 cgttaatttt tacagggaaa cctggcagct tgattggttg ttgcccgttg acaggagggg
     1801 atttacagcc gaagatgact atatttacac gttcagggat gaatttattg gtttccctaa
     1861 tgacaagtat gaagttgtcc acgaatttga aacagcaaac acggttttga tgaaaagatt
     1921 ggaaaagacc tctcaataat accaacgaag gttttcataa taagcggtcg atacgcagga
     1981 aattttccca atacttttgt ttgtccatct gttcgaaatg tatcattcca tacctgtact
     2041 gataaatctg aatctgattt accaatacgc aaatggcaat caggccaacg aaaatgcctt
     2101 ttacggcttt atttctgaat ccgtcaatag ccttagccag cagcaacata aataccggca
     2161 gaaactctat aagcacccgt gacgaaaaac tgccgccata ccaccagttc caccaggatg
     2221 acaacacgta aaataacacc agcacaaaaa acagcaaacc ataaaaaatg taccggtcag
     2281 ttttccagaa gaaataaaat cccgttaacg ataaaaacaa tatgggtgta tataaaaaag
     2341 cacctttttt ataactaaac aggaagtcaa acaggtgagg attagaaaaa ttaaaaaatt
     2401 cgcttccata gctgtcggta aaaaatgagc ccgtgctgat tttgtatagg ataggctgta
     2461 tggatattat cgcagcaaaa attgctagtc cggccaaggg ataccaggga tgcagaaaca
     2521 attcagacaa tcccttttta aatccctgcc aacctgtcgg aacaaaaaac agcgctaaaa
     2581 ggatgattcc attcacaggc ctcacaagca ttatcaggcc tagagcaa
//
LOCUS       DAOSFP010000331         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37768_length_2626_cov_3.954103, whole genome shotgun sequence.
ACCESSION   DAOSFP010000331 DAOSFP010000000
VERSION     DAOSFP010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37768_length_2626_cov_3.954103"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLI16_09010"
     CDS             <1..307
                     /locus_tag="PLI16_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84736.1"
                     /translation="AYTVGFGCSMIWFGSSAGVAITNKFPEARNVLLWLRKGWHVTLA
                     YVAGFFVLYMVVGWEPADNRVHKEPAISCPAQECGARDQANLLELQKSGVLDSLQIK"
     gene            375..746
                     /locus_tag="PLI16_09015"
     CDS             375..746
                     /locus_tag="PLI16_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84737.1"
                     /translation="MQKVKKVAGILVIAMFYCISAGFVNTNYDNAGNAVLSKAGMSAF
                     SSVAPLNMHCHTVPGDNQVKTISSMAAKFIRIEFCNFSVIGERICCTTEQQSGRSISC
                     VTGFLIQQHKRFIMFPFHNFW"
     gene            973..1308
                     /locus_tag="PLI16_09020"
     CDS             973..1308
                     /locus_tag="PLI16_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84738.1"
                     /translation="MSQHEFCGDLLLGMDENSGIVCFIMFTDDTVIREHIKLAEIADC
                     KVVNTSRSANGAVVIDKLELCFTAIEKTKPQVFWEFYNSHNNPTLIGELQLIGKWAEI
                     IKSKLKKSR"
     gene            2021..>2626
                     /locus_tag="PLI16_09025"
     CDS             2021..>2626
                     /locus_tag="PLI16_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84739.1"
                     /translation="MKKCLTIIVLIKCLIAGYACFAQNTIKVPADTMVFSGSFSSWTN
                     YNVKGMLPLQCGGRYLPALNYEIRFPHNNKLDFEVSLNFDGALSFHPFDSLQSGGNIA
                     PYRAWMRFSSNQWEVRAGLQKINFGSATLLRPLMWFDQVDPRDPLKITNGVWGLLFRY
                     YFLNNANIWVWGLYGNGKQRPWEIGKISQYYPEFGCRMQTPL"
BASE COUNT          766 a          494 c          581 g          785 t
ORIGIN      
        1 ggcttatacg gttggcttcg gctgctccat gatatggttt gggtcttcgg caggggtagc
       61 tattaccaac aagtttcccg aggcccggaa tgtgcttttg tggctcagaa aaggttggca
      121 tgttaccctg gcttacgtgg caggtttctt tgtcctctat atggtggttg gctgggagcc
      181 ggcagataac agggtgcata aagagccggc aataagttgc ccggcccagg aatgcggcgc
      241 acgcgatcag gccaatctgc tggagctgca gaaatcagga gtgttggaca gtctgcagat
      301 aaaatagttt atttcttgtg aaattttgac ggatttcctc cctggggaaa ctgccgcaaa
      361 atataattgc cactatgcaa aaggttaaaa aggtagcggg aatacttgtt attgctatgt
      421 tttactgcat ctcggcaggt ttcgtaaata caaactacga taacgccggg aatgcagtat
      481 tatccaaagc cggcatgagc gcattttcct ccgttgcccc gttgaacatg cattgtcata
      541 ccgttcccgg cgacaatcag gtcaagacca tcagttccat ggcggcaaaa ttcatcagga
      601 tagaattttg taatttttca gttattggtg agcgcatctg ctgtacaacc gaacaacaaa
      661 gcggcaggag catatcctgt gttaccggct ttctgataca gcagcataaa agatttataa
      721 tgttcccttt ccataatttc tggtaagcgc atacattatt tatcgaaatc tcttgaatgg
      781 tgccgcctgc gctgtactca aatattttat tatttaaatt taatcaatat gaataacaca
      841 accatcatat cgttagctat tcttttagca ctaactgtag tcctgattct tattttcacg
      901 attaaaaaac aaagtaaaat aaaaaagaaa ctgctcgtat tacaggatta tgcatcccaa
      961 aataattgta gtatgtccca acatgagttt tgtggcgatt tgcttttggg aatggatgaa
     1021 aactcgggta ttgtttgttt tattatgttt accgacgaca cagtaatccg ggaacatata
     1081 aagcttgcag aaatagccga ttgcaaagtg gtgaacacca gccgctctgc caatggagca
     1141 gtggtaattg ataagctgga gttgtgcttt actgctatag aaaaaacgaa gccacaggta
     1201 ttttgggagt tttataattc ccataataac cctaccctga ttggcgaact gcaattaatc
     1261 ggaaaatggg ctgaaatcat aaaaagtaaa ctgaaaaaga gccgctgata tccggaaaaa
     1321 tctaattgtt tttaatcaac aaccactttt gaccgattaa aaaatattaa acttgctgaa
     1381 acgcttgata atgttgtgtc cctgacggaa cttaccccat cgggcttgtt tgtcgctctg
     1441 ccaattttcc tgaggctgta tcaaaactag cttttgctga aaatcatcat taccccatcc
     1501 ctaaagggag tgctgatttt cagcgatttc caccctttag ggaggggaaa gaaggcggtg
     1561 aaaatcacaa agtaaacctt ttgagacagc cccaatatat ttgtagaata caagatataa
     1621 aacgtaatgt agtcccgtag ggacgaaata taatttaagc ggacaataat gatcaaaaac
     1681 atgaaaaaga ccgaagaaat tcggtctttt tttattttgg ctcgtgctaa ggcctgaaat
     1741 atgcagccat tgcggtattt agtctgattt attcattgat ggacgaagtg aaatttgagg
     1801 atgtgtgcgg acgttaaggc aagctgacta ggaacagctt gtcaaaagag taattgatga
     1861 agtacaaact ttgtgtatta gaaaaaccga tgataaaaaa gtttttttta cctttattaa
     1921 agtttgaaat gcttattttt atatctgaaa gtttaaaatt caatatgttc tgctgcaagt
     1981 aaatgttcag tttgacttat ttcatttcaa gtaaatgaaa atgaaaaaat gcctgacaat
     2041 catagtatta attaaatgtc ttatagccgg atatgcttgt ttcgctcaga atacgataaa
     2101 agtaccagcg gataccatgg ttttttccgg atctttttct tcctggacaa attataatgt
     2161 caagggaatg ttgcctttgc agtgcggtgg gcgttatctt cctgccttga attatgagat
     2221 aagatttccg cataataata aactcgattt tgaggtttca ctgaatttcg atggggcact
     2281 atcttttcat ccgtttgatt cccttcaatc cgggggcaac attgctcctt acagggcatg
     2341 gatgcggttt tcttccaatc agtgggaggt gagggcaggc cttcagaaaa taaattttgg
     2401 ctcggcaacc ctgctgaggc cgctgatgtg gttcgaccag gttgacccgc gcgatcccct
     2461 gaaaatcacc aatggtgtgt ggggcctgtt gttcaggtat tattttctta acaatgccaa
     2521 catttgggtt tggggattgt atgggaacgg aaaacagcga ccctgggaaa ttggaaaaat
     2581 tagccagtat tatccggagt tcggatgtcg catgcaaact ccattg
//
LOCUS       DAOSFP010000332         2625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_37788_length_2625_cov_3.856809, whole genome shotgun sequence.
ACCESSION   DAOSFP010000332 DAOSFP010000000
VERSION     DAOSFP010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37788_length_2625_cov_3.856809"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="PLI16_09030"
     CDS             complement(<1..1890)
                     /locus_tag="PLI16_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOH84740.1"
                     /translation="MMMVSNKKSLGRWSYFYKALLYIGAIVLIVLLFPKEYKFKYHFT
                     QDKPWVYEDLFAPFDFAVNKTEEELEKDRNDIIDKSIPYFITDTGVVPVRRRQLLIEL
                     GAGNSESGPNANIIRQKLGLSIFDSIYKKGVLRYDDILKNKDENFTIYLVNGNVVERR
                     TLSEIYNLEKAHDYILSQLHGKNGIDADFLLQALEKALIQNVSYSPEITNHENFLALK
                     SISSTKGYIQKNERIISKGELITPRYYQILLSLKKQYEGESSNIHSHFFIVLGQIILT
                     SIAMIMLVLFLLMFRADIIANTKKNLLILTVLMLVIVSASIIVRRYGYEYIYVLPLCV
                     VPLMIRTFFDTRLALFVHLISIILIGFLVPNGFEFIFLQLIAGIVTIISTVRLEKRSQ
                     FFITSLYIFCTYSLIYVGMTMMMSGSYKDIVLQNFMFFAFNSVLTLAAYPLIVLFERI
                     FGYVTNVSLLEYSNTNNTLLRELSQKAPGTFQHSLQVANLAEEAAHRIGANALLVRAG
                     AMFHDIGKVVSPVYFIENQLSSNNPHDKLSYAESAEIITRHIENGIILARKYGIPEQI
                     IDFIRTHHGTKRTEYFYRLYKNKNGEKIDESVFYYPGPVPFSKETAILMMADTVEAAA
                     KSLKVH"
     gene            complement(1921..>2625)
                     /locus_tag="PLI16_09035"
     CDS             complement(1921..>2625)
                     /locus_tag="PLI16_09035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH84741.1"
                     /translation="RLGDGLPTVDIMHYMPEGEDIFPYSFLEGSSLPTDFALLRGICR
                     KNNVEDYLEIGTWRGESVANVAPLAKNCYTLNLSDEEMRKSGLDEAYIAMHRFFSKDL
                     PNVTHLQANSMTFDFASLNKKFDLIFLDGDHHAEAVAHDTRKAFSLLKDEKSVIVWHD
                     YGLSTETPRYDVLSGILDGCPPNCIEHLYHVSNTLCALFTREKLPASFISPNTRPQKQ
                     FEIKIHALACNTDEKK"
BASE COUNT          766 a          544 c          551 g          764 t
ORIGIN      
        1 atgaactttt aaacttttcg ccgctgcttc tacggtatca gccatcatca gtatggctgt
       61 ttcttttgag aaaggtaccg ggcccggata ataaaaaaca ctttcatcaa ttttttcgcc
      121 gtttttattt ttatacaaac ggtagaaata ctccgttctt ttggtgccat gatgggtccg
      181 gataaaatct attatctgct cgggtatgcc atacttgcgg gccaggataa tgccattttc
      241 tatatgacgg gtaataattt ctgcactctc tgcataactc aatttatcgt gaggattatt
      301 gctactcagc tgattttcga taaaataaac aggacttacc actttgccaa tatcgtgaaa
      361 catggctccg gccctcacca gcaaggcgtt ggcaccgata cggtgcgcgg cctcttcggc
      421 caggttggcc acctgtaacg agtgttgaaa agtaccagga gctttctgcg acaattctct
      481 caacagcgtg ttgttggtat tggaatattc cagcagtgaa acattggtaa catagccaaa
      541 aattctctca aacaacacta tgagcggata agccgccagg gtaagtaccg aattaaaggc
      601 gaaaaacata aaattctgaa ggacgatatc tttgtaactg cccgacatca tcatagtcat
      661 gccgacatag atgagcgaat aggtacaaaa aatatacaga gaagtaatga aaaactgcga
      721 acgtttttca aggcgcaccg tgctgatgat ggttactatg ccggcgatca gttgtaagaa
      781 aataaattcg aaaccgttag gcaccaaaaa gccaataaga atgatactta tcagatgtac
      841 aaataatgcc agccgggtat cgaaaaatgt gcgtatcatc agaggcacta cgcacagcgg
      901 caacacatag atatattcgt agccgtaacg cctcacgatt atgcttgccg acactattac
      961 caacatcagg acagtaagaa taagcaggtt tttttttgta ttggcaatga tgtcggcacg
     1021 aaacattaac aggaacaaaa ccagcataat catagcaatg gatgttagta tgatttggcc
     1081 gagtacaatg aaaaaatgac tgtgaatatt gctgctttcg ccttcgtatt gctttttcag
     1141 ggatagcaat atctggtagt acctgggcgt gatcagttcg cccttcgaaa tgatgcgttc
     1201 atttttttgg atatatcctt tagtggaaga aatacttttc agagcaagga aattctcatg
     1261 attggtaatt tcggggctgt atgacacatt ttgaatcagt gctttttcga gcgcctgtaa
     1321 caaaaagtcc gcatcgatac cattcttccc gtgcaattgg ctcaaaatat aatcatgagc
     1381 tttttcaaga ttataaattt cggaaagcgt acgtctttcc actacatttc cgtttacaag
     1441 ataaatagtg aaattttcat ctttattttt caaaatatca tcataacgca gtacgccttt
     1501 tttatagata gagtcaaaaa tcgacagtcc gagtttttgc cgtatgatgt tggcattcgg
     1561 gccggattct gagttacctg ccccaagctc aatcagcagt tgccggcgtc gaactggcac
     1621 tacaccggta tcggtaatga aataaggaat acttttatcg ataatatcat ttcggtcttt
     1681 ttccagttct tcttctgtct tattcacggc aaaatcaaag ggagcaaaca ggtcttcgta
     1741 cacccagggt ttatcctgtg taaaatggta tttgaattta tattctttgg gaaacaacaa
     1801 aactatgagc acaatagcac cgatatacaa aagtgctttg taaaaatagg accaccgccc
     1861 gaggcttttt ttatttgata ccatcatcat tcaatagcct tttgaatctt gtaaaagtaa
     1921 ctattttttt tcatcagtat tacaagccag cgcgtgaatc tttatttcaa actgtttttg
     1981 tgggcgtgtg ttagggctaa taaaggatgc tggtaatttt tcgcgggtaa acaaggcgca
     2041 aagggtattg gatacatggt acaaatgttc gatacagtta ggagggcagc catcaagtat
     2101 gcccgacaac acatcataac gcggagtttc ggtgcttagt ccgtaatcgt gccagacgat
     2161 aaccgatttt tcatctttca gcaaactgaa agccttcctg gtgtcatgag caactgcttc
     2221 agcatgatga tcgccgtcga gaaagatgag gtcgaatttt ttattcagtg aggcaaagtc
     2281 aaaagtcatg gaattggcct gcagatgggt aacattaggc aagtctttgg aaaaaaaacg
     2341 gtgcatggct atatacgctt cgtccagtcc tgattttctc atttcttcat ccgacaggtt
     2401 tagtgtgtaa cagttttttg ccaatggtgc tacgttggcc acgctttcgc cccgccaggt
     2461 gcctatttcc aggtaatcct ccacattatt tttgcggcaa ataccgcgca gcaaggcaaa
     2521 gtcggtaggc agcgatgagc cttccagaaa gctgtatgga aaaatatctt ctccctcagg
     2581 catataatgc ataatatcaa ccgtgggcaa gccatctcca agccg
//
LOCUS       DAOSFP010000333         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_38182_length_2609_cov_2.207518, whole genome shotgun sequence.
ACCESSION   DAOSFP010000333 DAOSFP010000000
VERSION     DAOSFP010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38182_length_2609_cov_2.207518"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="amrB"
                     /locus_tag="PLI16_09040"
     CDS             complement(<1..993)
                     /gene="amrB"
                     /locus_tag="PLI16_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOH84742.1"
                     /translation="MKRIRLLTLLSLIFMTASCNSQNHEQPKNDRRPAVAGQFYAGDA
                     AMLNKDLKALFDKASPKKVNDVLAIISPHAGYVFSGEVAATAINQIDENKEYTDIFVI
                     GSSHSSYFSAASVYCDGDFITPLGTVEVDTDLAKKLVKENAIIKNYKDVHIFEHSLEV
                     QLPFLQYKMKKPFRIVPIVIGTSDAADCKKIAKALQPYLNSNNLFVISTDFSHYPNYE
                     NAKKVDHLTAQAIESNSPAQLLEALKYNEKLYIPNLATSLCGWSSVLTLLYMTENLDD
                     ITISTLQYKNSGDTPVYGDSLRVVGYTAMTLSGKIKNKMNEEFSLSSKDKEDLLD"
     gene            complement(1146..>2609)
                     /locus_tag="PLI16_09045"
     CDS             complement(1146..>2609)
                     /locus_tag="PLI16_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOH84743.1"
                     /translation="KIFSRITAVSVTAGLTLPANTLHQLFSQYVSQTRVPFVGEPLNG
                     LQIMGMLETRTLDFDTVFLLSVNEGVLPKAKSGTSFIPHGIRVDANLPTYKQNEEIFA
                     YHFYRILQRSKNIYLIYNTQPDDMGGGERSRFVNQVVHELPKKNPLVTIEELFVTFPP
                     EKNSIQPMAIKKDQAVAERLAALAKKGLSPSALNVYRKCGLQYYFSYVAGIWEAEELS
                     ETIDSKELGDHIHEVLDSLFKPYENGVLTEDVVSGFLDNYEAILEKVYYSDKPGIYDR
                     KSGKNLLIYNVIRDYLRKFILTQLEIVREHQKEKILHKVLSTEKDLRHSLELGDDSMV
                     KTLLLKGVADRIDLIGNNLQIIDYKSGNTGGKNFDIYKLLADEQDYRNDYCFQVLLYL
                     WLYNKQHPGERHEGQYCGVWSMRRIEEGISNVCYDEFPEKSRSPKLYAMQAEHLEAFE
                     QMLMNILKSLFNRDEDFVQTGNEENCELCPYALICGR"
BASE COUNT          660 a          626 c          557 g          766 t
ORIGIN      
        1 atccaacagg tcttccttgt ccttactgct caacgaaaat tcttcgttca ttttattctt
       61 gattttgcca ctcaaggtca tggcagtata gcctaccacc ctcaggctat cgccgtacac
      121 aggcgtgtcg cctgaatttt tgtactgcag cgtgctgatg gtaatgtcgt ccaggttttc
      181 tgtcatatac agcagggtga gcacagaaga ccatccgcac aaactggtgg caaggttcgg
      241 gatatatagt ttttcgttgt actttaatgc ttccagcagc tgcgccggcg agttgctctc
      301 gatggcctgt gccgtcaaat ggtccacttt tttggcattt tcgtagttgg ggtaatgcga
      361 aaagtcggtg ctgatgacaa acaggttgtt gctgttcaga taaggctgca gggctttggc
      421 aatttttttg caatcagcgg catcgctggt gccaatgaca attggaacaa tgcggaacgg
      481 ttttttcatc ttatactgaa gaaaaggaag ctgcacctcg agactatgtt caaaaatatg
      541 cacatcctta taatttttta ttattgcatt ttcttttacc agtttttttg ccaggtccgt
      601 atccacttcc acggtgccga ggggtgtaat aaaatcacca tcgcaataca ctgatgccgc
      661 gctgaaatag gagctgtgac tggagccgat gacaaaaatg tcggtatatt ccttattttc
      721 gtctatctga ttgattgcgg tggcagccac ttcgcccgaa aaaacataac ctgcatgtgg
      781 cgaaataatg gccaggacat cattgacttt ctttggcgac gccttatcaa acaaggcttt
      841 caggtcctta ttcaacattg cggcatcacc ggcatagaat tgccccgcca cggcaggccg
      901 gcggtcgttt ttgggctgct catggttctg agaattacaa cttgcggtca taaaaattaa
      961 acttaaaagg gttaataatc gtatcctttt catattgctc atttatacaa atatacgaaa
     1021 tttatagaca ttacggggca ggatttccgg attatttatt acaaatattc cccaccgcaa
     1081 taaagtatgt accagctaaa agcaatatac aactccttat tttttcacag taaccctcaa
     1141 caccattatc ttccacatat cagcgcatac gggcataact cacaattttc ttcgttaccg
     1201 gtctgaacaa aatcttcgtc cctgttaaaa agacttttaa ggatattcat gagcatttgt
     1261 tcaaaggctt ccaggtgctc tgcctgcatg gcatagagtt tgggcgacct gcttttctcc
     1321 ggaaattcat cataacacac attactgatc ccctcttcaa tccggcgcat gctccacacc
     1381 ccgcaatact gcccctcatg ccgttccccg ggatgttgtt tgttatacaa ccacaggtac
     1441 agtaaaacct ggaaacaata atcgttacgg taatcctgct catcagcaag cagtttgtaa
     1501 atatcaaaat tctttccgcc ggtattgccc gacttataat cgataatctg aaggttgttg
     1561 ccaatcaggt ctatcctgtc ggccacccct tttaacagca gcgttttcac catgctgtca
     1621 tcgcccagtt ccaggctgtg ccgcaggtct ttctccgttg aaaggacttt atgcaatatc
     1681 ttttcctttt gatgttccct gacaatctcc agttgcgtta gaataaattt tcgcaggtaa
     1741 tccctgataa cattatatat cagcaggttt ttgccgctct tgcggtcgta tatccccggc
     1801 ttgtcgctgt aataaacctt ttccaggata gcctcatagt tgtcaagaaa tccgctgaca
     1861 acatcctctg tcaacacccc attttcatac ggtttgaaga ggctgtccaa aacctcatga
     1921 atatgatcgc caagttcttt gctgtcgatg gtttccgata gctcttccgc ttcccatatc
     1981 ccggcaacat aactgaaata atattgcaaa ccgcattttc ggtaaacatt cagtgcggaa
     2041 ggcgaaagtc cttttttggc caaggccgcg agccgttctg ccaccgcctg atcttttttg
     2101 atagccatgg gctggatact attcttttcg ggcggaaaag taacaaacag ttcttcgata
     2161 gttaccaaag ggttcttttt gggcagctcg tgcaccacct gatttacaaa acggctgcgt
     2221 tcgccgccgc ccatatcgtc gggctgggtg ttatagatga ggtagatatt tttactgcgc
     2281 tgtaaaattc ggtaaaagtg atatgcaaaa atttcctcat tttgtttgta agtgggaagg
     2341 ttggcatcca cgcgaattcc atgagggata aatgaagtgc cggattttgc tttaggcagc
     2401 accccctcgt tgaccgacaa caaaaacacg gtgtcgaagt cgagcgtgcg tgtctcaagc
     2461 attcccataa tctgcaggcc attgagaggc tcgcccacaa aaggaaccct ggtttgcgaa
     2521 acgtactgag aaaacaactg atgaagcgta ttggccggga gtgtgaggcc tgccgttacg
     2581 cttaccgccg tgatacggga gaagatctt
//
LOCUS       DAOSFP010000334         2606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_38235_length_2606_cov_3.696198, whole genome shotgun sequence.
ACCESSION   DAOSFP010000334 DAOSFP010000000
VERSION     DAOSFP010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38235_length_2606_cov_3.696198"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2121)
                     /locus_tag="PLI16_09050"
     CDS             complement(<1..2121)
                     /locus_tag="PLI16_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOH84744.1"
                     /translation="MTDEINKHDEINKLAAESSERLKELSCINQTIQILKEEKPLRET
                     FEKICHVLQKSWQYPQFTEACITYDNMELSTPAYEKTAWRQLQPFKTIDDKKGFIQIC
                     YTKEFPEADEGPFLAEERNLIENIANIISGHINSLLARLILDKTKRIDVLDSELLQSE
                     TPQIRGRQLLQRFLNKQNYERDIFHDLMSFKVREILIVANLYDAYNIEKEGRFVEHFL
                     GEYYQLNLVSMPRVTGVSSAEEVFRELRGKHYDMVIFMMGVDRKIPSEISAEIKAGYP
                     YIPIFMMLNNYADIEFYEKNRNLFSAIDDVFVWNGDPKVFFAMINYVEDRVNVDNDTR
                     IGFVRVILLVEDSAIYYSKYLPLLYSNVMQQTQRIIEDVASDEVLKVLRLRARAKILL
                     ARNYEDAIRIFDKYKDFMLCLITDVKYEKDGQLDENAGVSLVQYARSRKKDLPIIMQS
                     TDSANADKAFDLKTIFIDKNSSTLLQDVQSFITHYLGFGNFIYKNAEGKQIAIARSLK
                     EFENYLKIIPDDSLVYHARRDHFSLWLMARGEVLLARYINPYKIPDFKTTQELRDFLL
                     HAIQMHRSEFNKGKVISYEEVDNLEESSIISLASGSMGGKGRGVAFINTLIYNFDFHN
                     LIPNINIRCPKTLIIGTDEFEYFLDRNKLRNIIATTNSYEEIRAEFVKGNLGDGLIKR
                     LRKILKLIKKPIAVRSSGLFEDSLA"
     gene            2308..>2606
                     /locus_tag="PLI16_09055"
     CDS             2308..>2606
                     /locus_tag="PLI16_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HOH84745.1"
                     /translation="MNTLNEKLEKFMAKVIAKNPAEPEFHQAVKEVAESLMPYIEENP
                     KYNGILERMVEPERVFIFRIPWVDDKGDFQMNRGFRVQMNSAIGPYKGGMRFHPTV"
BASE COUNT          726 a          536 c          517 g          827 t
ORIGIN      
        1 tgccagcgaa tcttcaaaca ggcctgaaga acgcacggca ataggttttt tgataagttt
       61 caggattttt cttaagcgtt ttatcaaacc atcacccagg ttgcctttca caaattcggc
      121 gcgtatctct tcgtaactat tagttgtagc aataatgttc cgcagtttat tcctgtcgag
      181 aaaatattca aattcatccg tgcctattat caatgttttc ggacaacgta tgttgatatt
      241 aggtatcagg ttatggaaat caaaattata aatcaatgtg ttaataaaag ccactccccg
      301 gcccttacct cccatagacc ctgaggccag gctgataatg ctgctttctt cgaggttgtc
      361 cacctcttcg taactgatga ctttgccttt gttgaactcg ctgcgatgca tctgtattgc
      421 gtggagcagg aaatcccgca gttcctgggt agttttgaaa tcaggaatct tgtaagggtt
      481 aatatagcgt gccagtagca cctcgcccct ggccatgagc cacagcgaaa aatgatcgcg
      541 tctggcatga tataccagcg aatcatcagg gattatttta aggtagtttt cgaattcctt
      601 aagcgagcgg gcgatggcga tttgcttgcc ttccgcattt ttataaataa aatttccgaa
      661 tcctaaatag tgtgtgataa aactctgtac atcctgcagg agtgtagaag aatttttatc
      721 aataaatata gttttcagat cgaaagcttt gtcagcgttg gcgctgtcag ttgattgcat
      781 gataatcggc aggtcttttt tcctgctgcg ggcatactgt acgaggctca caccggcgtt
      841 ttcgtctaat tggccgtctt tttcgtattt gacatctgtt atcaggcaaa gcataaaatc
      901 tttgtacttg tcaaaaattc tgatggcgtc ttcgtaattt ctggctaaca aaatttttgc
      961 cctggcccgc aggcgcagta ctttgagcac ttcgtcggag gccacatcct caatgatgcg
     1021 ctgagtttgc tgcatcacat tggaatacag caatggcagg tattttgaat aatagatggc
     1081 tgaatcttcc actaaaagta taaccctaac aaagccgatt cgcgtatcat tatcaacatt
     1141 tacgcggtct tccacatagt tgatcatggc aaaaaacact ttgggatcgc cattccacac
     1201 aaaaacatcg tcaatggcag aaaacaggtt tctgtttttt tcataaaact caatatccgc
     1261 gtaattatta agcatcatga atattgggat gtagggataa ccggctttga tttcggcact
     1321 gatttccgaa gggattttcc tgtccacacc catcataaaa atcaccatat cgtagtgttt
     1381 tcctcttaat tcgcgaaaga cctcttccgc tgacgacacg ccggtaacac ggggcatcga
     1441 caccagattc agctgataat attctcctaa aaaatgttcc acaaagcggc cttccttttc
     1501 aatgttataa gcatcataca ggttagccac aatcaaaatt tccctgactt taaaagacat
     1561 gaggtcgtgg aaaatatccc gttcataatt ctgcttgttc aaaaaccgtt gcaaaagctg
     1621 cctgcctctt atttgaggcg tttccgactg aagaagctcc gaatcaagga catcaattcg
     1681 tttggtttta tccagaataa ggcgtgccaa caggctgttg atatgccccg aaataatatt
     1741 ggcaatgttt tctatcaggt tacgttcttc agccaggaaa ggaccttcat cggcttcagg
     1801 aaactctttg gtataacaaa tttgtatgaa gccctttttg tcgtcgatgg ttttaaaggg
     1861 ctgtaattgc ctccaggcgg ttttttcata agcgggagtt gacaattcca tattgtcata
     1921 agtaatgcaa gcctctgtaa actgaggata ctgccaggat ttttgcagaa catggcaaat
     1981 tttctcaaag gtttccctaa gcggcttttc ttcttttaaa atctggatgg tctggttgat
     2041 gcaggaaagc tcttttaacc gctcggagct ttctgccgct aacttattaa tttcatcatg
     2101 tttgtttatt tcatcggtca taaaaataat tttgctttca agcctattct tttatcttca
     2161 aatttattaa ctttttttat caatacaatc aattactaaa attttattta ttttttttaa
     2221 tatttcaata aatttatttt gctttagtta atttttaact ttatttttgt attagacaaa
     2281 tagtttacaa atttaaaagg aaaaaacatg aatacactta atgaaaagct tgaaaaattt
     2341 atggcaaaag ttattgccaa aaatcctgcc gagcctgaat ttcatcaggc tgttaaagaa
     2401 gtggcagagt cgttgatgcc ttatattgaa gagaatccca agtacaacgg cattcttgag
     2461 aggatggtgg agcccgaaag ggtttttatt ttcaggatac cctgggttga cgacaagggt
     2521 gattttcaga tgaaccgcgg tttcagggta cagatgaaca gcgccatagg gccgtacaaa
     2581 ggcggcatgc gcttccaccc taccgt
//
LOCUS       DAOSFP010000335         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_38604_length_2588_cov_4.122385, whole genome shotgun sequence.
ACCESSION   DAOSFP010000335 DAOSFP010000000
VERSION     DAOSFP010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38604_length_2588_cov_4.122385"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLI16_09060"
     CDS             complement(<1..218)
                     /locus_tag="PLI16_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84746.1"
                     /translation="MKNIIRAAVLLVIPGMMLFSCKTAQQSGIPNCQLKTLQDTISYI
                     IGADVASNLRKNHFDINPDVFTYAFNKN"
     gene            complement(324..1067)
                     /locus_tag="PLI16_09065"
     CDS             complement(324..1067)
                     /locus_tag="PLI16_09065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH84747.1"
                     /translation="MKNIAYSISFLALAAFLFVSCGNSNKADLKTKNDSVAYVIGANI
                     GQNLQQNIAADSLEFNTNALIQGFKDALNGVDSLVFSKEDKQNIMMQFQKDMQQKQME
                     KMTKAAEPNKQAGLQYLEENKKQPGVTQTQSGLQYKVIKEGNGKTPTANDQVTVNYEG
                     KLLDGTVFDSSFERGKPATFPVTGVIPGWTEGLQLMKEGGSYELYIPSDLAYGDQGTQ
                     GIPGGSTLIFKVDLIKVEAAPANAQQPIQ"
     gene            1361..>2588
                     /gene="ispG"
                     /locus_tag="PLI16_09070"
     CDS             1361..>2588
                     /gene="ispG"
                     /locus_tag="PLI16_09070"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOH84748.1"
                     /translation="MFFIKSKLVLSRELHIGNLLLGGKNPVRVQSMTNTNTMDTAATV
                     SQTLQLATAGCDLVRITARNVAEAENLRKIKQALAEKNCFVPLVADIHFQPRAAEVAA
                     RIVEKVRINPGNYVDKISPGAKKIIYSENQYVKELEDISNRLKPLLGICREYGTAIRI
                     GVNHGSLSERVVSRYGDTVEGMVVSAMEYIDICRYHGFHNLVLSMKSSDVKTMIYAYR
                     QLVLRMTESGYDYPLHLGVTEAGAGKDARIKSGAGIGLLLMEGIGDTIRVSLTENPVH
                     EVPVALSLVKAFSAAKKNSAPSPKRAYEKQETINIGTIGGEQQPVCIFAGIEAKSCKE
                     PMPDFYWNKDGQCLTSPDNQQTVGLVYVDSKDLDDTGFLKKSCGEIPRVLFFDIGSTG
                     DLPALREKLTAWRQRGN"
BASE COUNT          699 a          509 c          622 g          758 t
ORIGIN      
        1 atgtttttgt tgaaggcgta tgtaaaaaca tccgggttga tgtcgaaatg attttttcgc
       61 aggttgcttg ccacatcggc gccaataata taactaatgg tgtcctgaag tgttttcaac
      121 tggcagttgg ggatgccgga ttgctgtgct gttttacacg aaaacagcat catcccgggg
      181 atgacaagca atacggctgc cctgatgatg tttttcatga tgatttttat cttaatttgt
      241 tgaattataa atgaatatgt tgaataaaaa agctgttgca aaaatacaac agctttttaa
      301 tatttctgta gaaataaaga ttattattga ataggctgct gggcattggc aggagcagct
      361 tctactttta tgaggtccac tttaaaaatg agtgtcgatc cgccggggat tccctgtgtg
      421 ccctgatcgc cgtaagcaag gtcggaaggg atatagagtt cgtaggatcc gccttctttc
      481 ataagttgca ggccttctgt ccatcccggg attacaccgg taacagggaa agtagcaggt
      541 ttgccgcgtt cgaaagaaga atcaaaaaca gttccgtcaa gcagtttgcc ttcgtagttt
      601 acggtaacct ggtcgttggc ggtgggggtt tttccgttgc cttctttgat gactttatat
      661 tggagtccgc tttgtgtctg ggtaacaccg ggttgttttt tgttttcttc gagatactgt
      721 aatccagcct gtttgttggg ttcagcagct ttggtcattt tttccatttg tttttgctgc
      781 atatctttct gaaactgcat cataatattt tgtttgtctt cttttgaaaa gaccaggcta
      841 tccacgccat tcagtgcgtc cttaaatccc tgaatcaaag cgttggtgtt gaattccagt
      901 gagtcagcag cgatgttctg ctgcaggttc tggccaatgt tagctccaat tacataagca
      961 acagaatcgt tttttgtttt caggtcagcc ttgttactgt ttccgcacga aacaaacaga
     1021 aatgccgcca atgcaaggaa agaaattgaa taagcgatgt ttttcatgat actttttttt
     1081 aaattttata aaatgtttgc aaatttacaa ataataataa tagtgcaagt ttttttataa
     1141 agtttggcac tatctttttt caggagttat tagcagaaat gactgaccgg attcaatgac
     1201 ttgcctgatg ttatttgatt ttggccccca aaaagatttg actaacaaaa gggttaccct
     1261 gacctgctgc ccgcaacacc cacacacatt cgtccatttc actacttcct ttatgaatag
     1321 gtcaggttca attcaatttt gatcaaaaaa taaagtaatt ttgtttttca ttaaaagtaa
     1381 actcgtgcta tcccgcgaac tacatatcgg caatttgttg ctgggtggaa aaaacccggt
     1441 acgtgtgcag tctatgacca atacgaacac catggacaca gcggctacgg tgagccagac
     1501 cctgcagctt gcaacggcgg gctgcgattt ggtcagaatc actgcccgta atgtcgctga
     1561 agctgaaaat ctgagaaaaa taaaacaggc tttagccgaa aaaaattgtt ttgtgccact
     1621 ggttgccgac atacattttc agcccagggc tgccgaggta gctgcgcgaa ttgtggaaaa
     1681 ggtcagaatc aatcctggta attatgtcga taagatatcg cctggcgcaa aaaaaatcat
     1741 atacagtgaa aaccagtatg tgaaagaact ggaagatatc agtaatcgcc tgaagccctt
     1801 gctcgggatt tgccgggagt atggcacagc gatacgcata ggggtaaatc atggctcgtt
     1861 gtcggagcgg gtagtatcac gttatggcga tactgttgaa ggtatggtgg tttcggctat
     1921 ggaatatatt gatatctgcc gttaccatgg ctttcacaat ctggtgctgt caatgaaatc
     1981 gagtgatgta aaaactatga tttatgcata ccggcaactg gttttgcgga tgacggagtc
     2041 gggttacgat tatccgctgc acctgggggt tactgaggcc ggcgccggaa aggatgcccg
     2101 gatcaaatca ggggcaggta tcggcttact gctgatggag ggcattggag acactatacg
     2161 tgtatcgctg acagaaaatc ctgtgcatga ggttcctgta gcgctatctt tggtaaaagc
     2221 cttttctgct gcaaaaaaga attctgcacc cagcccaaag cgtgcttacg aaaaacagga
     2281 aaccataaac ataggtacca ttggcggtga gcaacagcct gtgtgtattt ttgcgggtat
     2341 tgaagcgaaa tcctgcaaag agccaatgcc ggatttttat tggaacaagg atgggcaatg
     2401 ccttacaagc cccgacaatc aacaaactgt cgggttagtt tatgtagatt caaaagacct
     2461 ggatgatacc ggctttctga aaaaaagctg cggcgaaatc ccgcgggtgt tgttttttga
     2521 tatcggcagc acgggagatt tgcctgccct gcgggaaaaa ttgactgcct ggcggcagag
     2581 aggcaaca
//
LOCUS       DAOSFP010000336         2588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_38609_length_2588_cov_3.694433, whole genome shotgun sequence.
ACCESSION   DAOSFP010000336 DAOSFP010000000
VERSION     DAOSFP010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38609_length_2588_cov_3.694433"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="murB"
                     /locus_tag="PLI16_09075"
     CDS             <1..719
                     /gene="murB"
                     /locus_tag="PLI16_09075"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOH84749.1"
                     /translation="YCIKEQLSGLENLSGIPGQAGSSPIQNIGAYGVEVKSCIKEVLA
                     ISIENGEKLRFSNADCRFGYRDSIFKRDMKGRVIITNVVFCLSKKARFMLEYGGVAEE
                     VKRISDTPDLAAVSKAICNIRDKKIPNPKDIGSAGSFFKNPVIEKEAFEQLLARYPDI
                     SHYPAAEGRHKLAAAWLIDHCGWKGYRKDDTGVNDKQPLILVNYGQATGTEILHLSQQ
                     IQDSVHEKFGVYLETEVNIL"
     gene            772..>2588
                     /locus_tag="PLI16_09080"
     CDS             772..>2588
                     /locus_tag="PLI16_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOH84750.1"
                     /translation="MEILEHFQFPITDSVLTFLIVFIIIFFAPRLLKKLRIPGIVGFI
                     LAGVLIGPTGLNIISPKNGVEMFSSFGLLYIMFLVGLEIDMVDFKKHRSRSIVFGILT
                     FFIPITIGFAITYYVIKLDFLASLLVSSMFSTHTMVSYPIASRLGITKNKVINTVVGG
                     TIITDTAVLMLLAIIVKVFQGSLDTGFWIILFGYLAIFMVIMLWIVPAISRWFFKHLE
                     GDGGGQYIYLLCALFASAFLAEAAGIEPMIGAFMAGIALNSLIPSTSVLMNRTLFIGN
                     TIFIPFFLISVGMIVDLNVLLTGREAIIFAGILVVTAEATKFLAAYLTQKIYKFTPIE
                     RNVLFGLSSSHAAATIALILVGYNLGILNIDVLNGTVIVIFISCLISSFVTENAGKKL
                     ARIEKNRPKEEDNYQQRILVSISNPETMQSLINFALLVKGDRTKEPLYVLNVFTGNPD
                     TEESRYEIMVKNRQIENVLGYAVPDDKSARLVSRIDVNVANGINRAIKELMITKIIIG
                     WNGQSTTLLNLFGGIVENVLPKNNQMMFVTKIIHPLGYHKRLVLIVPPNAEYEPGYKK
                     WAHQVSAIAKELAAKVMIFANDETGHGIAELRAAVLEAA"
BASE COUNT          719 a          568 c          592 g          709 t
ORIGIN      
        1 catactgtat taaggaacag ctcagcggat tggaaaacct gtcgggcata cccgggcagg
       61 caggcagcag ccccatacag aatatcgggg cttatggtgt ggaagtaaaa agttgtataa
      121 aggaagtatt ggccatcagc atagagaacg gtgaaaagct gcggttttcc aatgccgatt
      181 gccggtttgg ataccgcgac agcatcttca agagggatat gaagggcagg gttatcataa
      241 ccaatgtggt attttgtctt tcaaagaaag cccggtttat gctggaatat ggcggagtag
      301 ctgaagaagt aaagcgtatc agcgacacgc ccgacctggc ggctgtgagc aaagccattt
      361 gcaacatacg cgataaaaag attcccaatc caaaggatat aggctctgca ggcagttttt
      421 tcaagaatcc ggtgatagaa aaagaagcat ttgaacagct ccttgcccgt tatcctgaca
      481 tttcgcacta tccggccgcc gaaggcagac acaaactggc cgcagcctgg ctgatcgatc
      541 actgcggatg gaagggctac cgcaaagacg acaccggtgt taatgacaaa cagccgctga
      601 tactggtaaa ttacgggcag gccaccggta cagaaatatt gcacctgtcg caacaaattc
      661 aagacagcgt acatgaaaag tttggcgttt atctcgaaac ggaagtaaat attctttgat
      721 tttcagccat atcgttcagg aaattttata tatttttgca aaaaatttcc tgtggaaatc
      781 ctagaacatt ttcagtttcc cataacggat tccgtcctta ctttcctcat tgtttttatc
      841 attatatttt tcgctccgcg tttgctgaaa aaactgcgga ttccgggcat tgtcgggttt
      901 attcttgccg gtgttttaat tggacccaca ggcctgaata tcatttctcc caaaaacggg
      961 gtagagatgt tttcgtcatt cggtcttttg tatatcatgt ttttggtagg actggaaata
     1021 gatatggtgg atttcaaaaa acatcgctcc cgaagcattg tttttggcat cctgactttt
     1081 ttcataccga tcacaatcgg ttttgcaata acatattatg tcataaaact ggatttcctg
     1141 gcttccctgt tggtttccag tatgttttca acacatacta tggtatccta tcccatagca
     1201 tcgcgcctgg gaatcacaaa aaacaaggtt atcaataccg tggttggcgg aaccatcatc
     1261 accgatacgg cagtgctcat gctgttagct atcattgtca aggtctttca gggttcgctg
     1321 gatacgggat tctggataat actatttgga tacctggcaa ttttcatggt catcatgctg
     1381 tggatagtgc ctgctatcag ccggtggttt ttcaaacacc tggaaggcga tggaggcgga
     1441 caatacattt acctgttgtg cgcccttttt gcttcagctt ttttggctga ggccgccggc
     1501 attgaaccca tgatcggggc atttatggct ggcatcgccc tcaattcgct gattccgtcc
     1561 acatcagtgc ttatgaacag aacattgttt attggcaaca ccatctttat accgtttttc
     1621 ctgatcagtg tagggatgat agtggatctg aatgtattac tgaccggaag ggaagcgata
     1681 attttcgcag gtattttggt agttaccgct gaagcaacca aattccttgc cgcgtacctc
     1741 actcagaaaa tttacaaatt cactcccata gaaagaaatg tccttttcgg cctgagcagt
     1801 tcgcatgccg cggctactat tgcacttatc ctcgtgggtt acaatctggg catcctgaat
     1861 attgacgtcc taaatggaac tgttatcgta atctttatta gctgcctgat cagttccttt
     1921 gtaacagaaa atgcaggaaa aaaactggca cgtatcgaaa aaaacaggcc aaaggaggaa
     1981 gacaattacc agcaaagaat acttgtttcc atttcaaacc cggaaaccat gcaatcgtta
     2041 ataaatttcg ctttgcttgt caagggtgac cgcacaaaag aacccttgta cgtgttgaat
     2101 gtttttacgg gtaatccgga taccgaagaa tcacgttatg aaatcatggt taaaaacagg
     2161 caaattgaaa atgtcctggg ctatgctgtt cccgatgaca agtcagcccg cctggtttca
     2221 cgcatagatg tcaatgtagc caacggtatc aaccgagcca tcaaggagct gatgattaca
     2281 aaaatcatca ttggatggaa tggccaatcc accaccctgt tgaatctttt cggaggcata
     2341 gttgaaaatg ttcttcctaa aaacaatcag atgatgttcg ttacaaaaat catacacccg
     2401 ctgggatatc acaagcgttt agtgcttatt gtgccaccca atgcggaata tgaacccggc
     2461 tataaaaaat gggcacatca ggtctcggcc attgccaaag aacttgcagc caaggtgatg
     2521 atttttgcca acgacgaaac cggccacggc atcgctgaat tgcgcgcagc ggtgctggag
     2581 gcggcgga
//
LOCUS       DAOSFP010000337         2585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_38668_length_2585_cov_3.779051, whole genome shotgun sequence.
ACCESSION   DAOSFP010000337 DAOSFP010000000
VERSION     DAOSFP010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38668_length_2585_cov_3.779051"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLI16_09085"
     CDS             complement(<1..518)
                     /locus_tag="PLI16_09085"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOH84751.1"
                     /translation="MKILKKIIISLLVLIALILLVLLFMPSTRHVEESINIKGPAKNI
                     YEQVVTIKNWEKWMPFSEGDPEMKTSYEGPAKGVGAIMKWESKIQGNGTMTIEEAEAY
                     KRIKLKLEFEGQGISHSGFSFTETGDSTNVVWSMDIENLKYPIGRLIGIFMPGVIHKS
                     FHNGLVKLKTVS"
     gene            complement(609..2222)
                     /locus_tag="PLI16_09090"
     CDS             complement(609..2222)
                     /locus_tag="PLI16_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84752.1"
                     /translation="MKKQILLLVCVALLAIPLVNYGQYTNDVTLNTQVSVLEDDVTGV
                     TGIAVTESGKTYITYLDGYFELKIQLLDDNGNKVFDSAGLVLETAATSYSVAKSTNII
                     TDASEQAIVAFWNWDASEIKLFSVSSTGTITDTLMCGTGSIPVLEPLPSGAFILAFSR
                     GDSTVIKKITSTAGLLATEYTRSVNYLCRDMVALPNGNFYMVSFDLLPNPGYAYTFAN
                     YIDGSTGNPLWANWTACSSNTNTGIYTANVACAVDNANFLYVSNTYFSGMNRTAFVQR
                     IDSTGNALWGVNGYTLITDGVFNYQQRIFTLYDNSSNELLCLINGQNVNDGSATNKIC
                     YQKISQNGIVQLPSSGIELAGASTNAALFGMDFCDNNIAFTYISGITNYIYASQIDPG
                     GTFQWSPNPLPLNITANAKSWVDALLKIKNNDIFIAFHETRSGHSGAYAQKAGCNGLL
                     PTGIINPESEPVPDALYPNPATEKITLECNNQDNSTVSVYSITGKLMLQKAISETKIT
                     LDISDLPQGLYMIKISNSSQPVLKKFVIE"
BASE COUNT          722 a          524 c          542 g          797 t
ORIGIN      
        1 tcgctgacgg ttttaagttt taccaggcca ttgtggaaac tcttatgaat aacgccgggc
       61 ataaaaatgc ctatcagcct gccaatggga tatttcaggt tttcaatatc catactccaa
      121 acaacgttgg tgctatcacc tgtttctgta aacgaaaaac cgctgtgcga aattccctga
      181 ccttcaaatt ccagtttgag ttttattctc ttgtatgctt cggcttcctc gatggtcatt
      241 gtgccattgc cctgtatttt gctttcccat ttcatgatgg cacccacacc ttttgccggg
      301 ccttcataac tggttttcat ttcaggatca ccctctgaaa atggcatcca tttttcccag
      361 ttctttatag taaccacctg ctcatagatg ttctttgcag ggcctttgat attgattgac
      421 tcttccacat gcctcgtgga gggcatgaat aataacacca gcaatataag cgctattaac
      481 acaagcaatg aaatgataat ttttttcaga attttcatag tgtggggttt ttgattaata
      541 aaatgttctt tgcaatagtg ttttctattg taaaagtaat atatagttat tgaaaaaatt
      601 tcttattttt attctatcac gaattttttt agcacaggct ggctggaatt tgaaatcttt
      661 atcatatata atccttgcgg taaatcgctg atatcaagag taattttggt ttcagatatt
      721 gccttttgga gcatcagttt gcctgttatg ctgtaaaccg aaacagtgga gttgtcctga
      781 ttgttgcact ctaaggtaat cttttcagtg gctggattgg gatagagggc gtcaggaaca
      841 ggttcggatt ccggatttat gatacctgta ggtaatagac cgttacagcc ggctttttgt
      901 gcgtaagcgc ccgaatgtcc gcttcttgtt tcgtggaaag caatgaatat gtcgttattc
      961 ttgattttta aaagcgcgtc aacccatgat ttggcgttag ctgtaatgtt gagcggcagc
     1021 gggtttggac tccactgaaa agttccgccc ggatctatct gcgaggcata aatataattt
     1081 gtgattcccg aaatataggt aaaagcaata ttgttgtcgc aaaaatccat gccaaacaaa
     1141 gccgcattgg ttgatgcacc ggcaagttct attcctgagg aaggcaactg aacaatgccg
     1201 ttttgagaaa ttttctggta acatattttg ttggttgccg agccatcgtt gacattctgt
     1261 ccattgatca ggcacaataa ttcattggag gaattatcat aaagggtaaa aatcctttgc
     1321 tgataattga atacgccatc ggttatcagg gtgtatccgt ttacacccca taaggcattg
     1381 ccagtactat caatgcgttg tacaaaagct gtccggttca tccctgaaaa atatgtgtta
     1441 gaaacataca ggaaattggc attatcaaca gcacaggcaa cattggccgt ataaatgcct
     1501 gtattggtgt tggaagaaca ggctgtccag ttggcccata aaggatttcc ggtgctaccg
     1561 tcaatataat tggcaaaggt gtatgcgtac ccggggttgg gcaacaggtc gaaagaaacc
     1621 atatagaaat tgccgttagg taaggccacc atatcccggc acaaataatt gacactgcgt
     1681 gtgtattctg tggctaacag gcctgcagtg ctggttattt tttttatgac ggttgaatcg
     1741 cctctgctga aagccagtat gaacgctcct gatggcagcg gctcaagaac gggaatgctg
     1801 cctgtgccgc acatcagtgt gtctgttatt gtaccagtgg aactcacgga aaataatttt
     1861 atttcgctgg catcccagtt ccagaaagcc actatggctt gctccgaagc atcagtaata
     1921 atattggtgc ttttggccac cgagtatgaa gtggctgccg tctccagcac caggcctgcc
     1981 gaatcaaaaa ctttattgcc gttgtcatcg agtaattgga ttttcagctc aaaatagccg
     2041 tccaaatagg taatgtaagt cttacctgat tctgtaactg caattccggt aaccccggtt
     2101 acatcatcct ccaataccga aacctgtgta ttcaaggtca catcattggt gtattgccca
     2161 tagtttacaa gaggaattgc caacagggca acacatacta aaagtaatat ttgctttttc
     2221 atgattggtt ttatttaggt taccagagat gtttattact attcacttcg aatgacaaag
     2281 aacgcttacc ggaaatttcc ggtaacaact tacaaattta ataaaatatt atgttaaaat
     2341 caagtattat tgcctgtttt tcaatacatt tagacaataa atttctaact ggccagaata
     2401 aaataccaca ggtcaaatac aggggtaaca aagtataaca atattaccag tgttgcaggc
     2461 gatgttactt agtgtcatta agaaaactcg tatttgttca aagaaaacgt caagtttgag
     2521 gcctgcccga ctacgccagt caggcgggct cgcgaacgga taaaaagcgg agtcccgaca
     2581 cttct
//
LOCUS       DAOSFP010000338         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39351_length_2555_cov_4.238400, whole genome shotgun sequence.
ACCESSION   DAOSFP010000338 DAOSFP010000000
VERSION     DAOSFP010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39351_length_2555_cov_4.238400"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PLI16_09095"
     CDS             complement(<1..1879)
                     /locus_tag="PLI16_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOH84753.1"
                     /translation="MKKYYLLILALCYSLTVFSQRQHEKDFIKHISQQEARSHENIFK
                     AGNTKLGDNYDLNYHRMHWFVDPSVNYISGSITSYFTALSTINQMVFELNSVLTIDSV
                     KYHNNQLTFSHPDNTFTIDFPTAIAAGAMDSVCVYYHGAPPQGAGFGSFIQSNHGGTP
                     IIWTLSEPFGAPDWWPCKNSLTDKIDSIDIYVTTTNGNRVGSNGLLISETVAGANKIF
                     HWRHRYPIATYLIAIAVTNYYAYSDHASLQNGTLEILNYVYPEDSSYARQNTPTLIPV
                     IQLYDSLFSDYPYLDEKYGHAQFNWGGGMEHQTMTFLVNYGFDLMAHELAHQWFGDKV
                     TCKSWHDIWINEGFATYLTGLTYYFLSNHAEWISWKADNIFYITTETWGSVYCDDTSN
                     VSRIFDGRLSYTKGAMVLNMLRFKMGDSAFFTCLKNYITDPLLAYGTASTDDFKAHAE
                     ASYGANLTDFFNDWIYNQGHPIYTLNSTIHADSSVSINFLQSQSHPSVSFFKLPVPVR
                     FSNGINDTTLVFDNTQNNQTFDIQLNFFPTQVTFDPEKELIAVLDTLIVNTGHCAAIE
                     NQLDIFPNPASEIITISYNGQQPEKVSIYDLSGALVTEYFPAVGGNSFVMDISGVNSG
                     FY"
     gene            complement(1891..2325)
                     /locus_tag="PLI16_09100"
     CDS             complement(1891..2325)
                     /locus_tag="PLI16_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84754.1"
                     /translation="MKIIKRHNLLIIVSLCALIFCSCKKEIQDPIPDVYVNFSMNISS
                     TFYIELSSVGGWVNVSGGYKGITVYRLSSEEFVAFERACPFDWKIDSAIVNVEPSGLI
                     LKCPACSSEFLIIDGSIVNGPSAIGLKKYNTNFDGQFLYIYN"
BASE COUNT          741 a          483 c          591 g          740 t
ORIGIN      
        1 aataaaatcc gctgtttacc cccgagatat ccattacaaa cgaattgcct ccaaccgcag
       61 gaaaatattc cgtcaccagg gcgccgctaa ggtcatatat actgactttt tcgggctgtt
      121 gtccgttata gctgatggta attatttcgc tggcgggatt gggaaaaata tcaagctggt
      181 tttcgatggc ggcgcaatgg cccgtgttta caatcaaggt atctaaaaca gcaataagtt
      241 ctttttcggg atcgaatgtg acttgtgtag ggaaaaaatt cagttgtata tcgaaggtct
      301 gattgttttg ggtgttatca aaaacaaggg tggtgtcgtt gatgccgttg gaaaacctta
      361 caggcacagg cagtttaaag aaacttaccg aaggatgact ctgcgattgc aggaaattga
      421 tgcttaccga actgtcagca tgaatggtgc tgttgagggt atagattgga tgcccctgat
      481 tatatatcca gtcattgaaa aagtccgtca ggttagcgcc gtacgatgct tcagcatgag
      541 ctttaaaatc atcggtagag gctgtgccat aagccagcag tggatcggta atatagtttt
      601 tcaggcaggt aaagaaagcg gaatctccca tcttgaatcg cagcatattt aagaccatgg
      661 ctcctttggt ataggacaac cttccgtcga agatgcgtga cacgttgctg gtgtcgtcgc
      721 aataaaccga accccaggtc tctgtagtaa tatagaagat gttgtctgct ttccagctaa
      781 tccactccgc atggttgctg agaaaataat aggtaagccc tgtcaggtag gtggcaaatc
      841 cttcattaat ccatatgtcg tgccagcttt tgcaggtaac cttgtcgccg aaccattggt
      901 gggccagttc atgggccatc aggtcgaagc catagttaac aaggaaggtc atcgtctggt
      961 gttccatgcc gccaccccag ttaaactgcg catggccgta tttttcatcc agatagggat
     1021 aatcggaaaa caagctgtca tacagttgga taacgggtat aagggtgggg gtgttctgcc
     1081 ttgcatagga agaatcttcg gggtaaacat agttcagaat ctccagggtg ccgttttgca
     1141 ggcttgcatg atcggaataa gcataataat ttgttacagc aatggcaatt agataggtgg
     1201 caatggggta gcggtggcgc caatgaaaaa ttttattggc tccggccacg gtttccgata
     1261 tcagcaagcc gttgcttccc accctgttgc cattggtggt agtaacatag atatctatcg
     1321 aatcaatttt gtcggtgaga gaatttttgc atggccacca gtcgggagcg ccgaaaggtt
     1381 cggaaagtgt ccagataatg ggcgttccgc catggttgct ctggataaaa gatccaaaac
     1441 ctgctccctg gggaggagcg ccatgataat agacacaaac agaatccatg gctcctgctg
     1501 caatagcagt tggaaaatca atagtgaagg tattgtcagg gtgactgaaa gtcaactggt
     1561 tgttgtgata ttttacgcta tcgatagtta acacgctgtt gagttcaaaa accatttggt
     1621 tgatggtcga tagtgctgta aaataagaag ttattgaacc actgatatag ttaacactgg
     1681 ggtcgacaaa ccaatgcatc cggtgataat tcaggtcgta attatctccc agttttgtat
     1741 tgccggcttt gaaaatattt tcatgactgc gggcttcctg ttgcgaaatg tgtttgataa
     1801 agtccttttc gtgttgccgc tgcgaaaaaa ctgtcaatga ataacatagg gcaagaataa
     1861 gcaagtaata ttttttcatg aatgtgaaaa ttagttataa atataaagaa attgaccgtc
     1921 aaaatttgta ttgtattttt ttaagccaat agccgaaggg ccgttaacta tcgaaccgtc
     1981 gataatcaaa aattcgctgc tgcatgccgg gcatttcagt atcagccctg atggctcgac
     2041 attaactatg gccgagtcaa ttttccaatc gaaggggcag gctctttcaa aagcaacaaa
     2101 ttcttcggat gataaacgat acacggtaat tcccttatag cctcccgaaa catttaccca
     2161 gccgccaaca gatgataatt caatgtaaaa agtcgatgaa atgttcatcg aaaaattcac
     2221 atatacgtcg ggtataggat cctggatttc ttttttacag gaacaaaaaa tcagggcaca
     2281 taaggaaact attattaata aattatgccg ctttattatt ttcattaaaa caatattggt
     2341 ttaaaaacgt tatttatggt gaataatttt gtgattattt tatttttttt gtcccgacgg
     2401 tatcgtaaaa cttaatattt ttatcgtttc taacgataag acaaaggtaa ccaaactata
     2461 aataacgatg aggtttttaa gaaatattat tttagtaata atggtgttta ctatcgcatt
     2521 gtccgggagc ggttgcagta gttttcagca ggcca
//
LOCUS       DAOSFP010000339         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39488_length_2549_cov_3.263031, whole genome shotgun sequence.
ACCESSION   DAOSFP010000339 DAOSFP010000000
VERSION     DAOSFP010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39488_length_2549_cov_3.263031"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..2033
                     /locus_tag="PLI16_09105"
     CDS             174..2033
                     /locus_tag="PLI16_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOH84755.1"
                     /translation="MNTVDYNEEAIRTLEWNEHIRQRPGMYIGKLGDGTSPDDGIYVL
                     LKETIDNAIDEFIMGTGKTIEITIKDQTVTVRDYGRGIPLNKLADCVSKINTGAKYGS
                     NSKVFIKTVGLNGVGSKAVNALSSYYVAQSVREQKIKTVEFAEGVQTKDHPITTTDLN
                     NGTIVKFTPDEKIFGNFHFIPEYVEKMLWNYVFLNNGLTIYFNGQKFVSKNGLLDLLN
                     NNIDGPTLYPVIHINENGFECAITHSATKYNEEYYSFVNGQHTTQGGTHQQAFREAVV
                     KTIREFYKKDFEPSDIRESIIAAISIKIQEPVFESQTKTKLGSINVEPNGATVRNYVQ
                     DVIKKHLDDYLHMNPETADILHRKILQSEKDRKELANIKKLAKDSIKKVSLHNKKLRD
                     CRIHYNSNDERRYESTLFITEGDSASGSITKSRDVNTQAVFSLRGKPLNCYGMTKRVC
                     YENEEFNLLQSSLNIEDEIENLRYNNIVIATDADVDGMHIRLLLLTFFLQFYPEVIRT
                     GHLYILQTPLFRVRNKKKTIYCYSDTERIKAIAELGSSPEITRFKGLGEISPDEFKHF
                     IGPNIRLEPVMLTKDSPVKDLLSFYMGKNTPERQDFIIENLRVELDVIKPATN"
     gene            2265..>2549
                     /locus_tag="PLI16_09110"
     CDS             2265..>2549
                     /locus_tag="PLI16_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84756.1"
                     /translation="MSKTNKKISRRQFIQTCMHYAGIAALSISGLDVFAGDKKNIAMN
                     NPADIWKWNTPAHYYTKTEKGIVCGLCHNACLITLNGKGMCRTRVNFKNEL"
BASE COUNT          833 a          641 c          489 g          586 t
ORIGIN      
        1 atctccaatc tctccaatct ctcaatctct caatctctca atctccaact ccctccaatc
       61 tctcccaatc tccccttttc tcaatcttta actcactatt gttaataaaa aaaatcccag
      121 tctatcctta ctctgacaaa gtcttcgtac ctttgcataa atttttcgca cccatgaata
      181 ctgttgatta taacgaagaa gccatccgca ccctcgaatg gaatgaacac atccgccagc
      241 ggccgggaat gtatattggc aagcttggcg acggcacctc gccggatgat ggcatctatg
      301 tgctgctaaa agaaaccatc gataatgcca ttgatgaatt tatcatgggg accggaaaaa
      361 ccatcgaaat aaccatcaaa gaccagacag ttaccgttcg tgactatgga cgcggcatac
      421 ctctcaataa actggccgat tgcgtatcaa aaatcaatac cggagcaaaa tacggatcca
      481 attccaaagt ttttattaag actgtagggc tgaacggcgt cggatccaag gccgtcaacg
      541 ccttgtcatc atactatgtg gcacaatctg tcagggaaca aaagataaaa accgtggaat
      601 ttgccgaggg agtccaaacc aaagaccacc ccattaccac aaccgacctg aataacggca
      661 ctattgtcaa attcaccccg gatgaaaaaa tttttggcaa ctttcatttt attccggaat
      721 atgttgaaaa aatgttgtgg aactatgttt ttctgaataa cggtctcaca atttatttca
      781 acggccagaa atttgtttca aaaaacggcc tgctcgacct gctgaacaac aatattgacg
      841 gccccaccct ctaccctgtc atccatatca acgaaaacgg ctttgaatgt gccataaccc
      901 acagcgctac caaatacaat gaagagtatt attcattcgt caacggccag cacaccaccc
      961 agggcggcac acaccagcaa gccttccgtg aagccgtggt gaaaaccata cgcgagtttt
     1021 ataaaaaaga ctttgaaccc agcgacatcc gcgaatccat catcgcagcc atcagcatca
     1081 aaattcagga acctgtattt gaatcgcaga ccaagacaaa actggggtcg ataaatgtgg
     1141 aacccaacgg cgccaccgtc agaaactacg tgcaggacgt cataaaaaaa catctcgacg
     1201 attacctgca catgaacccg gaaaccgctg acatactgca ccgcaaaatc ctgcagtcgg
     1261 aaaaagaccg taaagaactg gccaacataa aaaaattagc caaagacagc atcaaaaaag
     1321 tcagcctgca caacaaaaaa ctccgcgatt gccgcataca ttacaacagc aacgacgaaa
     1381 gacgttacga gagcaccctc ttcatcacag aaggcgactc ggccagcggc tccatcacca
     1441 aatcacgcga tgtgaacacc caggccgtgt tcagcctcag gggcaaaccg ctcaactgct
     1501 atggcatgac aaaaagagtt tgctacgaaa acgaagagtt taacctgctg cagtcgtcgc
     1561 tcaacatcga ggacgaaata gaaaacctgc ggtacaacaa tatcgttatt gccaccgatg
     1621 cagatgtgga cggcatgcac atacgcctgc tgctgctgac atttttcctt cagttttatc
     1681 ccgaagtgat acgaacagga catttgtaca tactccaaac tccgctgttc agggtaagaa
     1741 acaagaaaaa aaccatttac tgttacagcg acacggaaag aatcaaagct attgccgaac
     1801 tgggaagcag ccccgaaata acccgtttta aaggactggg agagatatca ccggacgaat
     1861 tcaaacattt tatcggaccc aacatcagac tcgaaccggt aatgctaacc aaagattcac
     1921 ccgtaaaaga cctcttgtcg ttttatatgg gaaaaaacac gcccgaacga caggatttta
     1981 ttatcgaaaa cctaagggtt gaactggatg tgatcaaacc tgcgaccaac taaaatctga
     2041 acattgacat tttagcggac tcagtaaatt aatctgaaga cacatttttt tacaccaaaa
     2101 tttttggtca tattactgcg ttactattga agtgagtcgg cataaaagcc gtcatgcttt
     2161 gttttgcata ttttgcagtc tgggcaaatt taaaaatccg gagaacagtt tttcgtcctg
     2221 atgatggctg ccggcaaaaa tatttttagt aattttacgg tataatgagc aagaccaata
     2281 aaaaaatcag ccgccggcaa ttcatccaaa catgtatgca ttatgcgggc atagcggctc
     2341 tgagtatttc aggcctcgac gttttcgccg gcgacaaaaa aaatattgcc atgaacaatc
     2401 ctgctgatat ctggaaatgg aataccccgg cgcattatta taccaaaacc gaaaaaggca
     2461 tagtatgcgg cctttgccac aatgcctgcc tgataacgct caacggcaaa ggcatgtgca
     2521 ggaccagggt aaatttcaaa aatgaactt
//
LOCUS       DAOSFP010000340         2541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39658_length_2541_cov_4.524940, whole genome shotgun sequence.
ACCESSION   DAOSFP010000340 DAOSFP010000000
VERSION     DAOSFP010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39658_length_2541_cov_4.524940"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1288
                     /locus_tag="PLI16_09115"
     CDS             110..1288
                     /locus_tag="PLI16_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOH84757.1"
                     /translation="MIPFDIALQTVLNNTKPLAGTESVKLDAALDRVLAMDVFSDMDM
                     PPFDKSAVDGYACRRQDVHNELKIVEVVPAGKAPEHNISENLCIKIMTGAMIPEGANC
                     VLMVEHTEKTKEGHIRFLKNDTQNNIAYQAEDIKKGALVLKKGILLKPEHIAVLASVG
                     CFEPQVSLMPRLSIISTGDELVEPNEVPGLSQIRNSNSWQLLAQAQQMKINAVYGGIA
                     RDDEEVTFRKLSKAADENDVVLLTGGVSMGDFDFVPEMIIKTGFEILFRTMAIQPGKP
                     TVFGMKGNKFCFGLPGNPVASFVIFELMVKPLLYKMMGHQFNPAIQSLPFAHDFKRRA
                     AERLSVIPVIVNREGTVATLDYHGSAHIHALVGANGLAFIPVGKTMVGKGELIDVRLL
                     "
     gene            1272..2072
                     /locus_tag="PLI16_09120"
     CDS             1272..2072
                     /locus_tag="PLI16_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH84758.1"
                     /translation="MLDSYKREINYLRVSVTDRCNLRCTYCMPADGVSLMRHEDILSF
                     EEISDIVKVAVSKGISKVRITGGEPLVRRGIISLIEQIAIIPGILDLSMTTNGILLEE
                     YAGQLAKAGIQRVNISLDTVDPQKYAEITRGGDIQKVVRGIAAAKKAGLSPIKINCVV
                     KNSSNEPDALGVKDFCEKNGLQVRFIQLMDLEKGHFSVVDGGSGGDCAICNRLRLTAD
                     GKIKPCLFNDIEFDVRTMGAEKAIAEAIKAKPECGTFNKNGRFNNIGG"
     gene            2072..>2541
                     /locus_tag="PLI16_09125"
     CDS             2072..>2541
                     /locus_tag="PLI16_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOH84759.1"
                     /translation="MWKKYFDKKNHKPRFGGLEILKYIGPGLLVTAGFIDPGNWVTNI
                     AAGSEFGYTLLWVVTLSTIMLIILQHNVAHLGIATGLCLSEAASKYTPAWVSRPSLAL
                     AMLASISTSLAVMLGAAIALKMLFGIPVPAGAIISAVFTLILLFTNSYKKIEKW"
BASE COUNT          704 a          543 c          640 g          654 t
ORIGIN      
        1 cggcggatcc gtgctctccg tgcctccgtg gttttccgac ccaaacacta ttcataaaaa
       61 cagcttcaag tcctaaagtt ttgtaatttt gtacataaag ctaattggaa tgatcccttt
      121 tgatatagcc ttacaaacgg ttttaaataa tacgaagcca ttggctggaa ccgaaagcgt
      181 aaaattagac gcggcattgg acagggtgct ggccatggat gtcttctcgg atatggatat
      241 gccgcccttc gacaaatcag cagttgatgg ttatgcatgc cgcaggcagg atgttcataa
      301 tgaactgaaa attgttgagg ttgtgccggc cgggaaagct ccggaacata atatttcgga
      361 aaacctgtgt ataaaaatta tgacaggggc catgatcccg gagggagcca actgtgtgct
      421 gatggttgaa cataccgaaa agacaaaaga aggtcatatc agatttttga aaaacgacac
      481 gcagaacaat atcgcttacc aggcagaaga tataaaaaaa ggagccctcg tgctgaagaa
      541 ggggatactg cttaagcccg aacatattgc cgtattggct tcggtgggat gttttgaacc
      601 gcaggtgagc ctcatgccac ggctgagcat catttccacc ggcgacgaac tggtggaacc
      661 taatgaagtt ccgggacttt cgcagattcg caactccaac tcctggcaat tgctggcaca
      721 ggcacagcag atgaaaataa acgctgtgta tggcggtatt gcgcgagatg atgaagaagt
      781 aaccttccgg aagcttagca aggccgccga cgagaatgac gtggtattgc ttaccggcgg
      841 cgtttcaatg ggagactttg attttgttcc ggaaatgata ataaaaaccg gcttcgaaat
      901 attattccgc accatggcca tacagccggg aaagcctacg gtttttggaa tgaaaggcaa
      961 caagttctgt ttcgggttgc cgggaaaccc ggtggcgtct tttgttattt ttgaattgat
     1021 ggtaaaaccg ttgttgtata aaatgatggg ccaccaattt aatccagcga tacaaagcct
     1081 cccttttgct cacgatttca agcgtcgggc ggccgaacgc ctttcggtga ttcctgtaat
     1141 agtaaaccga gagggcacgg ttgccactct tgattatcat ggttcagcgc acatacacgc
     1201 actggttggc gctaacgggc ttgcttttat ccctgttggc aaaacaatgg ttggtaaagg
     1261 agaattaata gatgttagac tcttataaac gcgaaataaa ttatctgcgg gtttcggtaa
     1321 ccgaccgttg caacctgcga tgtacctatt gcatgcctgc cgacggcgtc tccctgatgc
     1381 ggcatgaaga tattttaagt tttgaagaaa tcagcgatat cgttaaggtt gctgtctcaa
     1441 aagggatatc aaaagtgcgg atcaccggtg gcgagccatt ggtgcgcagg ggaattattt
     1501 cgcttattga acagattgcc atcattcctg ggatccttga tttgtccatg acaacaaacg
     1561 ggattttgct tgaagaatat gccggacagc ttgccaaagc cggaattcag cgggtgaaca
     1621 tcagtcttga tacggtggat ccgcaaaaat acgctgaaat tacccgtggc ggtgatatcc
     1681 aaaaagtggt aagaggtata gccgcagcaa aaaaggctgg tttaagcccg ataaaaatta
     1741 actgtgttgt aaaaaattca tcaaatgagc cggacgctct tggcgtgaaa gatttttgcg
     1801 aaaaaaacgg cctgcaggtc cgttttattc agcttatgga tcttgaaaaa ggccattttt
     1861 ctgttgttga cggggggtcc ggcggggatt gcgccatttg taaccgcctt aggctgacag
     1921 ccgatggaaa aataaaaccc tgcttgttca acgatataga gtttgatgtg aggacgatgg
     1981 gcgccgaaaa ggccatagct gaagccatca aagccaagcc cgaatgcgga acctttaaca
     2041 aaaacggtcg ctttaacaat atcggaggat aatgtggaaa aaatacttcg ataaaaaaaa
     2101 tcacaaacca cgttttggtg gtctggaaat actaaaatat atcggtcctg gcttgttggt
     2161 taccgccgga tttatcgacc ccggcaactg ggtaaccaat attgccgcag gttctgagtt
     2221 tggatacaca ttgttgtggg tagttaccct gtccaccatc atgttgatta tccttcaaca
     2281 taatgtggcg cacctgggca tagcaaccgg cttgtgtctt tcagaagccg cctcaaaata
     2341 tacgccggca tgggtgtcaa ggccttcgct ggcactggca atgctggctt ccatcagcac
     2401 ctcgctggca gtgatgcttg gtgcagctat agcccttaaa atgctctttg ggatacctgt
     2461 gccggccggc gcaattatca gcgctgtgtt taccctgata ttgttgttta ccaactctta
     2521 taaaaaaatc gaaaaatgga t
//
LOCUS       DAOSFP010000341         2534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39827_length_2534_cov_3.750706, whole genome shotgun sequence.
ACCESSION   DAOSFP010000341 DAOSFP010000000
VERSION     DAOSFP010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39827_length_2534_cov_3.750706"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="PLI16_09130"
     CDS             <1..866
                     /locus_tag="PLI16_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84760.1"
                     /translation="SYGQGFRFPSIGERYISTSAGNFGIYPNPDLKSETSWNAEVGLK
                     QLFKISKFVGFIDICGFWQEYQNYIEFNAGLWGRNLKPDSSGWDITKNLGYKFLNTAD
                     ARVWGIDVSLMGEGQFTKDFSMQVMGGYTFSRPTCLDPDKVYYTDYFISGNDTLSSQD
                     FTYMNTSSDTREHILKYRFEQLAKLDIQFTYKNFAWGVGGRYYGFMYNIDNFFMQNDR
                     PGLFNTGIKDYRKEHHNGDFVFDARVSYELKKHFKFTVLMTNILNTEYSLRPISVEPP
                     RVTSIQIVYKV"
     gene            872..1630
                     /locus_tag="PLI16_09135"
     CDS             872..1630
                     /locus_tag="PLI16_09135"
                     /inference="COORDINATES: protein motif:HMM:NF024599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCMD domain-containing protein"
                     /protein_id="HOH84761.1"
                     /translation="MKKLSYKALLLLTALLCTGILLPSCRKYIDEPEPVITELVCEKN
                     IRGGCLDQWTMVDDGFGFKSFEPLGNYLRTLNYLATLPAAAGGPGPLTTDSTSEAYEG
                     SYAAKLITGTFSPMGEPILIPGLVGTDSLDIPNATIRVGKRYTLKPLKFRGYYKYAPV
                     NGDSALVSVLLSKYNTALGKRDTIGLAREIYKNTVTEYTMIDLPINYQSQETPDTLTL
                     LICSSAGIRFDDLMNCKGQVGSTMWIDEIFFIMP"
     gene            complement(1668..1916)
                     /locus_tag="PLI16_09140"
     CDS             complement(1668..1916)
                     /locus_tag="PLI16_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH84762.1"
                     /translation="MMNTFEQLNAIFCKVFYDDDIKITRETTSEDVDGWDSLSHLNLV
                     VSVEKHFGIKFNDAEIAKWKNVGELMDSLEQLIQQKQH"
     gene            complement(1924..>2534)
                     /locus_tag="PLI16_09145"
     CDS             complement(1924..>2534)
                     /locus_tag="PLI16_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH84763.1"
                     /translation="PGIKIKPGSMGRPIDIIEAAVLSEGGRPCPVGETGHLCIKTPWD
                     SMLTAYLNNEAAFNEKIKNGYYYTGDMAFCDEEGYYHFVGRNDDVINTAGHLISPFEV
                     ESTLLEIPEIAESAVIGAPDELLFEKVVAFIRLKDHVVWTDDLELKIRIFLSNRLSSI
                     ATPQDIRLIEEVPKNKSGKIMRRVLKAWYMGEDPGDISTMDI"
BASE COUNT          704 a          581 c          552 g          697 t
ORIGIN      
        1 cttcctacgg acaagggttt cgttttccca gtattggcga acggtatatc tccacttcgg
       61 caggaaactt tggcatttat cctaatcccg acctgaaatc ggaaaccagc tggaatgccg
      121 aagtggggct gaaacagttg tttaaaatca gcaagttcgt gggattcatt gatatttgtg
      181 gattctggca ggaataccaa aactatattg agtttaacgc cggcctctgg ggaagaaacc
      241 tcaaacccga cagttcggga tgggacatca caaaaaacct cggttataag tttttaaaca
      301 ctgccgatgc acgcgtatgg ggtatagacg tttcgctgat gggcgaaggc cagtttacaa
      361 aagatttttc gatgcaggta atgggaggat atactttctc aaggcctacc tgcctggatc
      421 ccgacaaggt gtattatacg gattacttta tcagcggcaa cgatacgttg tcgagccagg
      481 actttacgta tatgaacacg tcttcggaca ccagagaaca catattgaaa taccgcttcg
      541 agcaattggc gaaattagac atacaattta cctataaaaa ttttgcctgg ggcgtgggtg
      601 gacgttatta cgggtttatg tacaatattg ataatttctt tatgcagaac gaccgccccg
      661 gcttattcaa tacgggtatt aaagactacc gtaaagaaca ccataacgga gattttgttt
      721 ttgacgcaag ggttagctac gaattaaaaa aacacttcaa atttactgta ttgatgacca
      781 atattctgaa cacagaatat tcgttgcgcc ccatttctgt ggagccaccg cgtgttactt
      841 ccattcagat agtttacaaa gtataacgtc tatgaaaaag ttatcttata aagctctttt
      901 gttattgaca gccttattat gcactggtat tttattgcct tcgtgcagaa aatatattga
      961 tgaaccagaa ccggtaatca ccgaactggt atgcgaaaaa aacatcaggg gaggctgcct
     1021 cgaccagtgg actatggtgg atgatggttt tggttttaag agttttgagc ccttgggcaa
     1081 ttaccttcgc acgttgaact atctggctac gctgccagct gctgccggag gccccgggcc
     1141 acttacgact gacagtacca gtgaggcata cgaaggcagt tatgccgcca aactgattac
     1201 gggaaccttt agcccgatgg gagaaccgat actaatcccc ggtctggtgg gcacagactc
     1261 tcttgacatt cccaatgcca ctatccgcgt gggaaaacgt tatacattaa agcctctcaa
     1321 attcagagga tattacaaat acgcccctgt taacggcgat tctgccctgg tttcggtgtt
     1381 attatcgaaa tacaacacag ccttaggaaa aagagacacc ataggattgg cccgtgaaat
     1441 atataaaaac acggttacgg aatacactat gattgacctc ccgatcaact atcaaagtca
     1501 ggaaacaccc gacacgctta cactgcttat ctgttcaagc gcaggtatcc gtttcgacga
     1561 cctgatgaac tgtaaaggcc aggtgggcag cacgatgtgg atagatgaga ttttcttcat
     1621 catgccgtaa ggcagcgaaa aaaacctttc aacccaaaaa tattatttta gtgctgtttt
     1681 tgctggatga gttgttccag cgaatccatc agttcaccca catttttcca ttttgcgatc
     1741 tcggcatcgt tgaattttat gccgaaatgt ttttccaccg aaaccaccag gttcaggtgt
     1801 gataaagaat cccagccatc aacatcttct gaggtggttt ccctggtgat ctttatatcg
     1861 tcgtcataaa atactttgca aaaaattgca ttcagttgtt caaatgtatt catcataaaa
     1921 tgtttaaatg tccatagttg aaatgtcgcc ggggtcttcg cccatatacc aggctttcag
     1981 cacacggcgc atgatttttc cacttttgtt ttttggcacc tcttcaatca gacggatatc
     2041 ctgcggcgtg gcaattgaag acagccggtt ggacaaaaaa atgcgaatct tcagttccag
     2101 atcgtccgtc cacacaacat ggtctttgag ccgtataaag gccaccacct tttcgaaaag
     2161 caactcatcg ggcgctccga taacagccga ttctgcaatt tcgggaattt ccagcagagt
     2221 gctttctacc tcgaacggac tgatcagatg accggccgta tttataacat cgtcgttacg
     2281 tcccacaaaa tgatagtaac cctcttcatc acagaaagcc atatcgcctg tataataata
     2341 accgtttttg attttttcgt taaaggcagc ctcattgttc agataggctg taagcatgga
     2401 gtcccagggc gttttaatgc acagatggcc ggtttctccc acagggcatg gtctgccgcc
     2461 ttcagacaaa acggccgctt cgatgatatc aatgggccgc cccatcgaac ccggcttaat
     2521 ctttattccc gggc
//
LOCUS       DAOSFP010000342         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39863_length_2533_cov_3.100484, whole genome shotgun sequence.
ACCESSION   DAOSFP010000342 DAOSFP010000000
VERSION     DAOSFP010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39863_length_2533_cov_3.100484"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..2159
                     /locus_tag="PLI16_09150"
                     /pseudo
     CDS             255..2159
                     /locus_tag="PLI16_09150"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
     gene            2167..>2533
                     /locus_tag="PLI16_09155"
     CDS             2167..>2533
                     /locus_tag="PLI16_09155"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH84764.1"
                     /translation="MDNDSGTSVFKTDACFDTAPASGYHLIAGLNSHNLQMLVFDPEK
                     NMLQKISILYFDKNFSLVKRLDAQSAVWANDLWLFQDVLERDFFSDGTTKAKHLKIKK
                     ISINKTPDDFKASRKEGEEM"
BASE COUNT          750 a          573 c          560 g          650 t
ORIGIN      
        1 agggcgaaaa tgcgtttacc tcgcggagta tgtattataa ttttgatacc cgcaaggggc
       61 tggtcaagga tatttttacc aacgagggcg aaagctacct gcatggcagc gttgtaaaac
      121 gttttcctga cggcaaggca aatttcaaaa aaggcgcttt caccacgtgc agtctcgaaa
      181 atccgcatta tcagatacgc ttcagcaagg ccaggatgat acccaacgac aaatttgttt
      241 cggggccggc ctatatggaa attgaacatg tgcccacgcc gctcctgctg ccgtttggat
      301 tttttcctaa caaaaggggg taaatatcgg gaattatcat cccaacctgg ggagaatcga
      361 ccaacagggg cttttatttt gaacgcggcg gctactattg gggtattaat gactacctgg
      421 atatgatttt tcagggagac atctataccc gtggaagttg ggcgctgtac ctgacaacaa
      481 attacaagaa aaggtacaaa tacgatggca gcatcaaact ttcctacgcc gtaaatataa
      541 tcagccagaa gtttttgcct gattataaca aattcaagga tttttcggtg gaatggtcgc
      601 acaagcagga cgccaaggcc aggcccaaca gcagcttttc ggctcaggta aaaatggcca
      661 gcagcagctt ttacaagttc aacccgtcgt caaccaacga ttatttgtcg aacacctttg
      721 agtcgagtat tgcctatcag acttcgctct ttggaggcaa aagcaacctc tcgctgaacc
      781 tggggagccg gcagaacgtg aacaccaagg aacttcagtt gtggctgccg caggttaatt
      841 tctcagtcaa tacgttttat cctttccgga aaaaaaataa agccggcgcc atgaaatggt
      901 gggacaatat caacgtgaac tattcgctta acgcgcaaaa ctatatcaac acctacgaca
      961 gcctggtgct gactcccaga atctgggata tgatgcaaaa cggcatacag cattccatac
     1021 aggtgcgcaa ccccattaaa ttgctgaagt acatcagctg gaataactcg gtgaatttta
     1081 cagaacgctg gtattttcag tcggtcaacc agcagtggtt caacgatgtt gacacatcct
     1141 atatcaaaaa agacaccatc aggcgttttc agatggccca cgaaatcaat attgcgtcgc
     1201 agctttccac caagatttac ggaatcttcc gctttaaaaa aggcgctttg caggccatac
     1261 gccatgtgct gacgccttcg gttactttcg gctatcatcc tgattatggc tctaaaatat
     1321 ggggttacta taaatatgca caggtcgatt ccaccggccg caccatgagg tattccatct
     1381 tccagaatgg catttacggc accccccccg atggcctgtc gggaaaagtg agttttaacc
     1441 tgaacaacaa ccttgagatg aaggttcgtt caaaaaaaga cactgtcacc gggtcgaaaa
     1501 agattgtact gcttgattac ctgaatattg gttcgggtta tgactttgcc aaagattcgc
     1561 tgcgctggga ttacctttcg ctcaatatac gtacacggct ttttaaccgg ctggacgtga
     1621 gtttcaacgg tgcctgggac ccttatgtga tagatacgaa cggcaaccgc ctgaaccggt
     1681 ttgaatggaa cgaaaaccac cgcttgttcc gccggcagaa tacctcgtgg atcatgggta
     1741 tctcgtattc gatcagcgat aacgacttca agaaaaaaga caaaaaaacc aacgagaaaa
     1801 aaagtgtctt cctgaacaaa tggaacatca gcatgaatct gaacttcagc tattccagcc
     1861 ggttcgatta ccggacgatc agctatatcc ccgacacggt tttaacggtg atggtcaacg
     1921 gagagtttta tattactccc aagtggcggg tgaatttcac gacaggttat gattttaaat
     1981 caaaagactt ttcctatact tccttaagca tccaccgtga tttgcattgc tgggagatgt
     2041 tcatggagtg ggtaccatac ggtttccgaa aaagttataa ttttaccatc cgaattaaat
     2101 cttccatgct gaaagacctt aaactcacca ggaagagcga ttggagagat aatttttaat
     2161 tgccatatgg ataacgattc cggcacttcg gtatttaaga cagacgcctg cttcgacacg
     2221 gcgccggctt cgggctatca tttgatagca gggctcaaca gccataatct gcaaatgctg
     2281 gttttcgatc ccgaaaaaaa catgttgcaa aaaatatcaa ttctttattt cgataaaaat
     2341 ttttcccttg taaaaagact cgatgcacaa agtgcggttt gggctaatga cctttggtta
     2401 ttccaagatg ttcttgaacg agattttttt tccgatggaa caacaaaagc aaagcatctt
     2461 aaaataaaaa aaatttcgat caacaaaaca cctgatgatt tcaaggcttc ccgcaaagag
     2521 ggggaagaga tga
//
LOCUS       DAOSFP010000343         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39934_length_2530_cov_4.037172, whole genome shotgun sequence.
ACCESSION   DAOSFP010000343 DAOSFP010000000
VERSION     DAOSFP010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39934_length_2530_cov_4.037172"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PLI16_09160"
     CDS             <1..1476
                     /locus_tag="PLI16_09160"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOH84765.1"
                     /translation="KKYWITQQLNYDWLLPNYPSEKVGVVMRGRSYISEVAFDTVLKN
                     RDFGPGNIVFDEAAGKKDDDYWKARRQDTLTQKELKTYQKIDSIGKKKNFDYYSFAID
                     KVLEGYVPVSFLDFSLSHLFNYNSIEGIRVGLGLRTNEKMCRWLTVSAFGGYGFWDKQ
                     FKYGGGLEFTLSKKHEIFLGAAYKKDLEMPGMTDFLGYNNFSYWNEYLINRMDYVEQL
                     KAYFNFRFLRYAQGGITLLHENRQPVYPYVYTPSVSDTFAKNNFSFSEVKMGFRYAYD
                     EKLIDAFNKRYTLGTKYPILYVSYIHGFRGLLGGQFAYDKLEIAVEKEFMIKHLGKTS
                     IMAEGGYIWGNAPYMKLFKGRGSYSSSFVFYLRNSFQAMRVDEFTYDKYVALHFRHSF
                     GSYLFNIKKFRPELSICQSILYGELSNKDAHPGNNFFQVPDEWYFESGLILDNLARLK
                     LFNLAYLKMGVGVFYRYGYYAFEDPLKNIAVKFSFKLSGSR"
     gene            complement(1529..>2530)
                     /locus_tag="PLI16_09165"
     CDS             complement(1529..>2530)
                     /locus_tag="PLI16_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOH84766.1"
                     /translation="YLSLRFQEKYIYSNCDKVDHIYHKAADSYLQHDLSVAFNFGKAK
                     DTDQDGIPDSKDKCPDVKGLLKFDGCPDTDNDGVTDADDKCPGTPEGVKVDSKGCPLD
                     TDGDGIADYMDKCPDVKGLAKFEGCPDSDGDGVIDNNDKCPGTPEKVKVDTKGCPLDT
                     DGDGVVDYLDKCPDVKGLANLDGCPDRDGDGVADYLDKCPDVKGIAANKGCPEVKQEV
                     KEIFKKALQGIEFETGKDIIRPVSFPILNNVVKIMKENKEYNLLINGHTDNVGDDQSN
                     MTLSQKRAAAVMAYLVNKGVEKERMKSFGFGETKPVADNNTAAGRAKNRRVEFVVEF"
BASE COUNT          643 a          528 c          605 g          754 t
ORIGIN      
        1 aaaaaatact ggattacgca gcagctgaac tacgactggc tattgccgaa ttatccttca
       61 gaaaaggtgg gggtggtgat gcgcggaaga agctatatct cggaggttgc atttgatacg
      121 gtgttaaaaa acagggattt tggcccgggc aacattgtat ttgatgaggc ggcaggaaaa
      181 aaagacgacg attactggaa agcgcgccgg caggatacgc tgactcaaaa ggagctgaag
      241 acctatcaga aaatagattc tattggcaaa aaaaagaact tcgattatta ttcgtttgcc
      301 attgataaag ttcttgaagg ttatgtgcca gtgagttttt tggatttctc gctgtcacat
      361 cttttcaatt acaacagcat tgaggggata cgggtgggac tgggtttgcg caccaacgaa
      421 aaaatgtgcc ggtggttgac cgtcagcgct tttggcggct atggtttttg ggataaacag
      481 tttaaatacg gcggcgggct ggagtttacc ctgtcaaaga aacacgaaat ttttcttggg
      541 gctgcctata aaaaagattt ggaaatgccc ggaatgactg attttcttgg gtacaataat
      601 ttttcttatt ggaatgagta cctgattaac aggatggatt atgtggaaca attgaaagcg
      661 tattttaatt tcaggttttt gcgttatgca cagggaggaa taacattgct gcacgaaaac
      721 cgccagcccg tgtatccata tgtgtacaca ccttccgtaa gcgatacctt tgccaaaaac
      781 aacttcagct tttcagaagt aaaaatgggt ttccgctatg cttacgatga aaaacttatc
      841 gatgctttca ataaaagata taccctgggc acaaaatatc ctattttgta tgtcagctat
      901 attcatggct tcaggggctt gctgggcggg cagtttgcat acgacaagct tgaaattgcc
      961 gtagaaaaag agtttatgat caagcacctg gggaaaacca gtatcatggc cgaaggtggc
     1021 tatatctggg gaaatgcgcc ctacatgaaa ctgttcaagg gccggggttc ctatagttcc
     1081 agtttcgtct tttatttacg aaactcattt caggcaatgc gcgttgatga gtttacgtat
     1141 gataaatacg tagcccttca tttcaggcat tcgttcggaa gttatttgtt caacataaaa
     1201 aaattcaggc cggaactgag tatatgccaa agcatattgt acggcgaatt atcaaacaag
     1261 gatgcccatc cggggaataa tttttttcag gttcctgacg aatggtattt tgaatcggga
     1321 ttgatacttg acaacctggc ccgcctgaaa ttatttaacc tggcatacct gaaaatgggt
     1381 gtgggtgttt tttaccgcta tggttattat gcttttgaag accctttgaa aaatatcgcg
     1441 gtaaagttta gtttcaaact atcggggagt agataaaaaa aaatgaccga tcagcagtaa
     1501 tcggtcattt ttctatctgt taaaaaaatc agaattcaac cacaaattcc acgcgtcggt
     1561 ttttggctct gccggcagcg gtgttgttat ctgccacggg ttttgtttcg ccaaagccga
     1621 atgatttcat gcgttctttt tccacgccct tgttgaccaa gtatgccatg acggctgcgg
     1681 cgcgtttttg cgaaagcgtc atgttcgact ggtcatcgcc cacgttgtcg gtatggccgt
     1741 tgatgagcag gttgtattcc ttgttttctt tcataatctt aacaacattg ttcaatatcg
     1801 ggaagcttac agggcgaatg atatccttac ctgtttcaaa ctcgatgccc tgcagtgctt
     1861 ttttgaagat ttcctttact tcctgtttta cttcgggaca tcctttgttg gcagcaattc
     1921 cttttacatc ggggcatttg tcgaggtagt cggcgacacc gtcaccatcc ctatcggggc
     1981 agccatcgag attggccagg ccttttacat cgggacattt atctaaataa tcaaccacgc
     2041 cgtcgccatc tgtatccagc gggcatcctt tggtatccac ttttaccttt tcgggtgtgc
     2101 cggggcattt gtcgttattg tcgataacgc cgtcaccgtc ggagtcgggg catccttcaa
     2161 attttgccag gcctttcacg tcgggacatt tgtccatata gtcggcaatg ccgtcgccat
     2221 ctgtatccag cggacatcct ttgctgtcta ctttcacgcc ttcgggggta cccggacatt
     2281 tgtcgtcggc atcggttacg ccgtcattgt cggtgtcggg gcatccgtcg aatttcaaga
     2341 ggcctttcac gtcgggacat ttatctttgc tgtcgggaat gccgtcctgg tcggtgtctt
     2401 tggctttgcc aaaattaaag gccacactca gatcgtgctg caaataactg tcggcagctt
     2461 tatgataaat atggtccacc ttgtcgcaat tggaataaat gtatttttcc tgaaaacgca
     2521 ggctcaggta
//
LOCUS       DAOSFP010000344         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39935_length_2530_cov_3.831111, whole genome shotgun sequence.
ACCESSION   DAOSFP010000344 DAOSFP010000000
VERSION     DAOSFP010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39935_length_2530_cov_3.831111"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..873)
                     /gene="lipA"
                     /locus_tag="PLI16_09170"
     CDS             complement(4..873)
                     /gene="lipA"
                     /locus_tag="PLI16_09170"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOH84767.1"
                     /translation="METKPVKPERKPDWLRIKLPGVNDYSYIKDYLSDFQLHTICESG
                     KCPNMAECWEQKTATFMILGNSCTRACRFCAVSKSQPLAPDPDEPRKLAEAIKKIGLK
                     HCVITSVTRDDLPDGGAGIWHDAIIAIKQKNPGITIEALIPDFNGNHDSLDLLVKAQP
                     DIISHNLETVSRLTPEIRIKARYALSLEVLHYLACKGITTKSGIMLGLGETPEEIYET
                     MDDLLNVQCRIITIGQYLQPSKKHFPVKKYYSPAEFEQFKKIALEKGFEFAESGPLVR
                     SSYHASKHIKTDN"
     gene            complement(879..1628)
                     /locus_tag="PLI16_09175"
     CDS             complement(879..1628)
                     /locus_tag="PLI16_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84768.1"
                     /translation="MNFSRKILLLSILLLSGPVIFAQSESNIDNKILYRKERSGFIMV
                     HSGGFGVGYRSGKHKTYFRKFMWEVEGLNIKHPKEHKTQSYYENSKNFIYGKLNSFFA
                     VRGGVGQQHILNGKPYWGGVEVRLFYFGGLSLGITKPIYLNIVKFDETTGQSYLSLER
                     FDPNIHSVYDIYGKASFFKGFNKIGVYPGIYTKVGLSFEYGAEDTFLKALECGVFADC
                     FYKNVPIMAFQKNQFLFANVYLSLHFGVRKN"
     gene            complement(1689..1820)
                     /locus_tag="PLI16_09180"
     CDS             complement(1689..1820)
                     /locus_tag="PLI16_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84769.1"
                     /translation="MKKTLSVILLLVAVAMVISSCATRELCPAYSKEIKKPATEKRV"
     gene            2099..>2530
                     /locus_tag="PLI16_09185"
     CDS             2099..>2530
                     /locus_tag="PLI16_09185"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH84770.1"
                     /translation="MNPQIEESWKTRLMQEFTAQYFSQLKEQLTEEKKRFVIYPPGSL
                     IFSAFDHTPFDAVKVVIIGQDPYHGAGQANGLCFSVSNGIPKPPSLENIFKELKTDLG
                     ISVPVTGNLEKWANQGVLLLNASLTVRANTANSHQNLGWHTF"
BASE COUNT          692 a          496 c          548 g          794 t
ORIGIN      
        1 gtgtcagtta tcggttttga tatgcttgct tgcatgatat gatgagcgga ccaacgggcc
       61 ggattctgca aattcgaacc ctttttccag tgctattttt ttaaactgtt caaattcggc
      121 aggggaatag tattttttta cgggaaaatg ctttttcgag ggttgaagat attgccctat
      181 ggtgatgata cggcattgaa cgtttaacag atcgtccatg gtctcgtaaa tctcctcagg
      241 ggtttctccc agtccgagca tgatgcctga ttttgtggtt attcctttgc aggcaaggta
      301 atgtaacacc tcgagactga gcgcgtaccg ggctttgatg cgaatctcag gagtgagtct
      361 gctgacggtt tcaaggttgt gcgaaatgat atcgggttgt gcttttacca acaggtccag
      421 gctgtcgtgg ttgccgttaa aatcaggtat taatgcctca atggtgatgc cgggattctt
      481 ttgtttaatg gctataatgg cgtcgtgcca gatgccggcg ccgccatcgg gcaggtcgtc
      541 gcgggtaact gaggtgatca cacaatgttt caagcctatt ttttttatgg cttctgccag
      601 ttttcttggt tcatcagggt cgggggccag gggttgtgat ttgcttacgg cacaaaaacg
      661 gcaggccctt gtacagctgt tcccaagaat cataaaagtg gcagtttttt gttcccagca
      721 ttcggccata ttgggacatt ttccgctttc acatatagta tgaagttgaa aatcagataa
      781 ataatctttt atatatgaat aatcattaac acctggcagt ttaattctga gccagtctgg
      841 ttttctttcg ggcttcacag gtttagtttc catcgatatt agttttttct cactccaaaa
      901 tgcaggctca ggtaaacatt tgcaaataag aactgatttt tctgaaaggc cataatgggc
      961 acatttttat aaaaacagtc cgcaaaaaca ccgcattcga gtgcttttaa aaaagtgtct
     1021 tccgcaccgt attcaaaact caatccaact ttggtataaa ttcccggata aacgccaatt
     1081 ttgttaaacc ctttgaaaaa tgatgccttg ccatagatgt catagacact gtgtatattg
     1141 ggatcaaagc gttccagaga aaggtacgac tggcctgtcg tttcgtcgaa ttttactatg
     1201 ttcaggtaaa tgggtttggt gattccgagc gaaaggccgc caaaatagaa tagcctgact
     1261 tcgacaccgc cccaatatgg cttgccgttt aagatgtgct gctgtcctac accgccacgg
     1321 actgcaaaaa aactgttgag ttttccgtaa ataaaattct ttgagttttc gtagtacgat
     1381 tgggtcttat gttctttggg gtgcttgatg tttaagcctt ccacttccca cataaatttg
     1441 cgaaaatatg ttttgtgttt gcccgaacgg taacccactc caaatcctcc ggaatgtacc
     1501 atgataaaac cggaacgttc cttacggtac aggattttat tatcaatatt ggattcggat
     1561 tgcgcgaaaa tgacaggccc ggataaaagc aaaatggata gaagcaatat tttccgggaa
     1621 aaattcatac aaacaaatag ttttgaataa gagccgctta taaaattgcc ggaaatactg
     1681 atgttgtgct acactctttt ttccgtggcc ggttttttta tttctttact gtaggcgggg
     1741 cataactctc tggtggcaca ggatgaaata accattgcta cagcaactaa taaaagtatt
     1801 acagacagtg tttttttcat tttgatcagt attttgaatg agatctgtgt gatttatatg
     1861 catcgcagtg tacctgagtc tggcatgaag aaagaacaac tatgattgtt gctatcagaa
     1921 tacagactgc aattatcttt ttcattgatt attaatgctt atattgtgta gcaaaaatac
     1981 acattttttc cgacataatg agatcagtct tttgatttat taacaatttt atgttgaaca
     2041 atattaatat ctactttaat aacgttattt ttactgcaaa attttttcga tggctactat
     2101 aaatccacaa atagaagaaa gctggaaaac caggctgatg caagagttta cggctcagta
     2161 tttcagccag ttgaaagaac aactgacaga agaaaaaaaa cgatttgtta tatatcctcc
     2221 cggatcactt atattttcgg ctttcgacca tacgcctttc gatgccgtga aagtggtgat
     2281 tatcggccag gatccgtatc atggagcggg acaggctaat gggttgtgct tttcggtttc
     2341 caatggcatc ccgaagccgc cttcgcttga aaatattttt aaagaactta aaacagacct
     2401 cggaatatct gtccccgtga cgggaaatct cgaaaaatgg gccaatcagg gcgtgctgct
     2461 gttaaatgct tcgcttaccg tcagagccaa tacggctaat tcgcaccaaa atcttgggtg
     2521 gcacactttt
//
LOCUS       DAOSFP010000345         2529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_39957_length_2529_cov_4.337914, whole genome shotgun sequence.
ACCESSION   DAOSFP010000345 DAOSFP010000000
VERSION     DAOSFP010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39957_length_2529_cov_4.337914"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1437
                     /locus_tag="PLI16_09190"
     CDS             262..1437
                     /locus_tag="PLI16_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HOH84771.1"
                     /translation="MKNTLQNKFWIILTAFFLLSVYLVMFSCKKSDPLPEPIDVGYNP
                     TPYTIARPWYFPTTMNIPADNPMTVEGVELGRYLFYDGRLGGRSHPDSLMSCATCHLQ
                     ENAFECGINHPEYLGGFTHGITGVSTPHVMLPLFNLVYNSNGYLWNGIIHPSNPNTSR
                     RRLEDLCWMGVHAPHEMYSDTNKSKALIGSIPGYKPLFKKAFGTEEITFERMAKAISQ
                     FIRSIVSYNSKCDKWLRGEGSLTPSELNGFVLFVTEEGADCFHCHGGDGNPLFTTNLF
                     YNNGTDSVFNDPRDRYSVTGDPMDHGAYKAPTLRNIELTGPYMHDGRYATLDEVIEFY
                     SHHLIWSPYINPLMHHIGENGAQLTTQEKADLKAFLLTLTDETLLTNPDYGPPASLP"
     gene            1479..>2529
                     /locus_tag="PLI16_09195"
     CDS             1479..>2529
                     /locus_tag="PLI16_09195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOH84772.1"
                     /translation="MKIRIAFFILFIALITHSRAQEANDTILLNTVEITEYATKKIPF
                     IRNIVVKSEMEENSLRDVGDALRSIPNVSGLRKGGANIDPVVRGFKYSQLMTLINGAI
                     EIEGGCPNRMDPTTSHIDIDDVEQIEIIKGPYALRYGPSFGGVINLSTYQPKFGEKFE
                     IHSRISTGYESNWNGNKEHLAVTGGTKKINFLLTGNRLQYGNYDDGNDNIYKSSFLKY
                     SYSGKIGFSPVDKHNFSLFYQGTMHKNVYFPALPMDEREDNSNIMGLTYTTNQQQGIF
                     SGAYFKANYAMVNHIMDNKSRANADTNMVAVSDVDANTLGIRTGVNLQFKDRYFLFFG
                     ADYQYINKDGDRTKIL"
BASE COUNT          791 a          569 c          499 g          670 t
ORIGIN      
        1 aaagttatag atttttatct ataatcttgt ccgactattt tgggggctaa tacaacgata
       61 taaagtatat tctataatta cagaataggg ggcgtgattt tttgcccgaa taattattcc
      121 gatacgatat ctgaaatagt aaaatagtgt ctttacaccc tgaactgtga aatgcttttc
      181 catactggta ttttaaaatg aacaaaattc cctatctttg tatttagaaa aaatattgaa
      241 tagcgatgtt atcctgaaaa aatgaaaaac acattacaaa ataaattctg gattatttta
      301 accgcatttt ttttgttatc ggtttattta gtaatgtttt catgtaaaaa aagtgaccct
      361 cttcccgaac cgattgatgt tggctacaac cctacaccat ataccattgc aaggccatgg
      421 tattttccca ccactatgaa tatacctgcc gacaacccca tgacggttga aggagttgaa
      481 ctcggccgct acctttttta cgacggtcgt ttgggaggac gctcccaccc cgactcactg
      541 atgagttgtg ccacctgcca tctgcaggaa aatgcttttg aatgcggaat aaatcatccc
      601 gaatatctcg gagggttcac ccatggaata acaggggttt ccactccgca tgttatgttg
      661 ccgcttttca atcttgtata taattccaac gggtatttat ggaatggaat catacatccc
      721 agtaacccca atacttctag aaggcgtctc gaagacctgt gctggatggg cgtgcatgcc
      781 ccccacgaga tgtatagcga caccaataaa tcaaaagcgc tgataggcag catcccggga
      841 tataaaccgc tgtttaaaaa agcctttggc accgaagaaa ttacttttga acgtatggct
      901 aaggcaatat cacagtttat ccgcagcata gtttcatata attcaaaatg tgataagtgg
      961 ctcagaggcg aaggatcgct gactccatcg gaacttaatg gctttgtatt gtttgttacc
     1021 gaagaaggtg cagattgttt tcattgccat ggcggagatg gcaatccgtt gtttaccacc
     1081 aacctgtttt acaacaacgg caccgattct gtttttaatg atccccggga caggtattcg
     1141 gttactggcg accccatgga ccacggggca tacaaagcgc ccaccctgcg caacatcgag
     1201 ctgacaggtc cttatatgca cgatggccga tatgctaccc tggatgaagt tatcgaattt
     1261 tacagccatc acctgatatg gtctccatat atcaacccat tgatgcacca cattggtgaa
     1321 aacggggcac aactaactac ccaggaaaaa gcagacttga aggcttttct cctgacattg
     1381 actgacgaaa ccctgctgac caatccggac tatgggccac cagccagcct gccataaaaa
     1441 catattgaaa aataacaact atgtataaac tataactcat gaaaatccga attgcttttt
     1501 ttattctttt tatcgcattg ataacgcatt ccagggcaca ggaagcaaat gacaccattc
     1561 tacttaacac ggttgaaatt accgaatatg ccacaaagaa aatacctttc atcagaaata
     1621 tcgttgtaaa atccgaaatg gaagagaatt ccctgcgcga tgtgggcgat gcactacgaa
     1681 gcattcccaa tgtttcaggc ctgcgaaaag gcggagcaaa catcgaccct gtagtccgcg
     1741 gattcaaata cagccaactg atgacattga ttaacggagc catagaaatt gaaggaggct
     1801 gtcccaaccg catggatccc accacttcac acatcgatat tgatgatgtg gaacaaatag
     1861 aaatcatcaa agggccttat gccttgcgtt atggcccttc gtttggcggg gtaataaatc
     1921 tgtcaaccta ccagccaaag ttcggcgaaa aatttgaaat ccattccagg atatcaactg
     1981 gctatgaatc aaactggaat ggcaacaaag aacatctggc ggttacaggc ggaacaaaaa
     2041 aaatcaattt tctgctaacc ggcaaccgat tgcaatatgg aaattacgat gatggaaacg
     2101 acaacattta taaatcctca tttctgaaat acagctacag cggaaaaatc ggcttctccc
     2161 ctgtggacaa acacaatttc agcctttttt accagggaac gatgcataaa aatgtttatt
     2221 ttcccgccct gcccatggac gaacgcgaag acaattccaa catcatggga ctgacctata
     2281 ccacaaacca gcaacaggga atattcagcg gggcctattt taaagcaaat tatgccatgg
     2341 taaatcacat catggataac aaatccaggg ccaatgccga caccaatatg gtcgccgttt
     2401 cggatgtaga tgccaatacc ctgggcatac gcacgggagt aaacttacaa ttcaaagacc
     2461 gttatttctt gttttttgga gctgattatc aatatattaa taaagatggc gaccgtacca
     2521 aaatactaa
//
LOCUS       DAOSFP010000346         2525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_40035_length_2525_cov_3.875304, whole genome shotgun sequence.
ACCESSION   DAOSFP010000346 DAOSFP010000000
VERSION     DAOSFP010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40035_length_2525_cov_3.875304"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PLI16_09200"
     CDS             complement(<1..457)
                     /locus_tag="PLI16_09200"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH84773.1"
                     /translation="MIDLKNVFPEKNTLPDRVVLDKPIEQREYLINGEILQWQGDFQQ
                     VFSPVYFGNDTQGAYIGSYPLMDARESLKALEAAVQAYDHGRGEWPTMSVEKRIHCVK
                     KFVVMMQEQRELIIRLLMWEIGKNLKDSEKEFDRTVVYINDTIESLKNLD"
     gene            894..>2525
                     /locus_tag="PLI16_09205"
     CDS             894..>2525
                     /locus_tag="PLI16_09205"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH84774.1"
                     /translation="MSDFRKIILIVLLSLGCCYVLKAQKVALVLSGGGAKGCAHIGVL
                     KALEENCIPIDYIAGTSIGAIVGAMYASGYTPEQIEKIFTTEEFINWAKGTIEDKYKF
                     FYKEDAPDASWINFKFNYDTILSYKLPTNIISPYQVDFGMMYFLAAAGEAAGNNFDSL
                     FIPFRCVAADVAEKKAISFASGYLPEAVRASMTYPFYFKPIKIDNKLLFDGGMYNNFP
                     SDVALEDFNPDIIIGSNVASNWSASSIDQDNVVEQIENMLTTNTNYSVLCDNSVMISP
                     PLPKMNLLNFDNAKEIIDIGYYAALDKIPDIRLFVVDSLSKDQRNAKRKKFLNKKPPM
                     LYDSVKIEGINPLQSVYTRNSIMHQDSVLTVEQVKEEYFRLIADDKIESIYPKTYYNK
                     KTGYFTLNLDVKRDKNFVAQLGGLISSSSITGAYIGLQYKYWGLTGSKLSVNFYIGRF
                     YSSAMIKNRFDFATRVPFSLETSFAWNKWDYFKTTTRFFEDAMPSYLIENEINFMAEL
                     AFAAGNKAKFIVGPAIGQIRNNYYQTNSFSRTDTAD"
BASE COUNT          757 a          513 c          496 g          759 t
ORIGIN      
        1 tgtcgagatt tttcaacgat tcgatggtat cgttgatata tacaacggtc cggtcaaatt
       61 ccttttctga atcttttaag tttttcccga tttcccacat cagcagcctg ataattaatt
      121 cacgctgctc ctgcatcatc acgacaaact tttttacaca atgtatcctt ttttctaccg
      181 acatggtggg ccattcgccc cggccatgat cgtaggcctg taccgctgcc tcaagagcct
      241 tgagcgattc gcgggcatcc attaacggat aactgcctat gtaagcacct tgagtatcat
      301 tgccaaaata taccggtgaa aatacttgtt gaaaatcgcc ctgccattgc agtatttctc
      361 cgtttataag atattcacgt tgctcaatcg gcttatctaa aacaacccta tccggcaggg
      421 tgtttttttc aggaaacaca ttttttaaat ctatcataat ttatatgtta atgttttttt
      481 gcaattctgt tgtcacacca gaatgtttga tactggcata cgaaggtaat atatatgttt
      541 gaatttctgc acggcttatt tttaccctga tttttattcc ggctaaaata gtacatacca
      601 tagagcaagc ggggcgcttt aacacagttt ttgtaaaatt tttgctgggt tttcccgaca
      661 ggagtccgtt tgacgggttt ccccggggtt aaatccggaa ctcatatgat taaatatggg
      721 gttaaattca agcacaataa taccctgcaa tattttttta tgaaaaaaac gttagatatc
      781 cgacaaattg atatttttgt atgacaagaa actaccataa aaagtagcat tataaaccat
      841 aaaagaggta tgggttaatg ctttttttgt atttttaagg ttttaataac atcatgtctg
      901 atttccgaaa aataatactg atagtgcttt tgagcttggg ctgttgttac gtgctgaaag
      961 cacagaaagt agccttggtt ttgagcggtg gaggcgccaa aggctgcgcc catattggcg
     1021 tattaaaagc cctggaggaa aactgcatac ccattgatta tattgccggc acatccatag
     1081 gagctattgt gggtgccatg tacgcctctg gttatacgcc ggagcaaatt gaaaaaattt
     1141 ttaccactga agagtttatt aactgggcca aaggaaccat agaagataaa tataaatttt
     1201 tttacaagga agatgcgccg gacgcctcat ggataaactt caagttcaat tatgatacaa
     1261 ttttaagtta caaattaccc acaaacatca tttcacccta tcaggttgat ttcggcatga
     1321 tgtattttct ggccgctgcg ggcgaagccg cgggcaataa tttcgatagt ttgttcatcc
     1381 ccttccgctg tgttgcagcc gacgtagccg aaaaaaaagc tatttccttt gccagcggct
     1441 atcttcccga ggctgtaagg gcttcgatga cttatccttt ttactttaag cccataaaaa
     1501 ttgataacaa gctgttgttc gacgggggta tgtacaataa tttcccttcg gatgttgccc
     1561 tcgaagattt caatcccgac atcattatcg gtagtaatgt ggcttcaaac tggagtgcgt
     1621 cttcaataga ccaggataat gttgtggaac aaattgaaaa tatgttgacc accaatacca
     1681 attacagcgt attgtgcgac aacagcgtaa tgatttcgcc gccactgccc aaaatgaacc
     1741 tgcttaattt cgataatgcc aaagaaatca ttgacatcgg ctattatgca gctctcgata
     1801 aaataccgga tatcaggttg tttgtggttg actctttaag caaggatcaa agaaatgcca
     1861 aacgaaaaaa gtttctgaat aaaaaacctc caatgcttta tgactcagta aagatagaag
     1921 gaataaaccc actgcaatcg gtgtatacaa gaaattctat catgcaccag gattcagtcc
     1981 tgactgttga acaggtaaaa gaagaatact tcaggctgat agccgatgac aaaatagaat
     2041 cgatttatcc aaaaacttac tataacaaaa aaacagggta ttttacatta aatctcgacg
     2101 taaaaagaga caaaaatttt gtggcccagt tgggagggct gatttcgtcg agcagtatca
     2161 caggggcata tatcggtttg cagtacaaat attggggact tacaggatca aaactctccg
     2221 ttaattttta tatcggtcgg ttttacagtt cggccatgat aaaaaacaga ttcgactttg
     2281 ccacccgtgt tccattttca ttggaaacat cgtttgcgtg gaataaatgg gattatttca
     2341 agaccaccac tcgtttcttt gaagatgcaa tgccttcata tctcatcgaa aacgaaatca
     2401 actttatggc cgaactggct tttgccgcgg gcaacaaggc caaattcatt gtagggcctg
     2461 ctattggaca gatacgaaac aactattacc aaaccaacag tttttcccgt acagatactg
     2521 ccgac
//
LOCUS       DAOSFP010000347         2515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_40272_length_2515_cov_3.938211, whole genome shotgun sequence.
ACCESSION   DAOSFP010000347 DAOSFP010000000
VERSION     DAOSFP010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40272_length_2515_cov_3.938211"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PLI16_09210"
     CDS             complement(<1..1373)
                     /locus_tag="PLI16_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOH84775.1"
                     /translation="MVHSTKNFIIYKASAGSGKTYTLVKEYLKLVLTDPSKYRHILAV
                     TFTNKAAYEMKSRIMSSLKMLSGLGEDTVTEDEKKSVRKKNGALISELCASSGLDEKG
                     LAARSGSILHKILHNYSDFAISTIDSFVQKIIRTFAYDLKISQNFEVELDSDRVLSEA
                     VDVLISYAGSDDQITRMLIGFIEQKMDDGQNWDISQDLKTFSRQLLKEDTQKFLHLFG
                     DVKLANFSKAISGISARRAATDNNARKLAGAVLKLIEDNDINPKSFYQGMRGIPGFFT
                     KVSQNGLAGSSPNSYCNSAIQEDMWYSKTQPENEKNKIDGVKDQIAEAYNAIIPMIRE
                     FRDLAVIVKSLYPLSLLNQIEKETEALKSDANLIFISDFYRKIHEQLLGEPVPFIYQR
                     AGEKYAHYFIDEFQDTSVLQFQNMLPLIENALASGNKNLIVGDGKQAIYRWRDGEVMQ
                     FATLPAI"
     gene            1654..1845
                     /locus_tag="PLI16_09215"
     CDS             1654..1845
                     /locus_tag="PLI16_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004570569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOH84776.1"
                     /translation="MKKGTVKFFNELKGFGFIKDNESDKEYFVHASGLIDKIKEGDTV
                     SFELTEGKKGLNAVNVKLV"
     gene            2117..>2515
                     /locus_tag="PLI16_09220"
     CDS             2117..>2515
                     /locus_tag="PLI16_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84777.1"
                     /translation="MKKLVFLLFSMVISCLANGQTTLISPAGDGGFESGGTPAANNWY
                     AVNSTTDGWYVGSVPVPATGTNCAYISSAAGTSWTYNETSAIQHIYYDVIIPAGESKL
                     TLTFKWKALGEGSGFSDWDNMKVFFGAGSAM"
BASE COUNT          712 a          482 c          531 g          790 t
ORIGIN      
        1 tagatggcgg gcagagtcgc aaactgcatc acttctccat cgcgccagcg ataaatagcc
       61 tgtttgccat caccgacaat caggtttttg tttcccgaag ccagcgcatt ttcgataagc
      121 ggcaacatat tctgaaattg cagcacggag gtgtcctgaa actcatcaat aaaataatga
      181 gcatattttt cgcctgcccg ctggtagata aaaggaacgg gttcgccaag caactgttcg
      241 tgaattttgc ggtaaaagtc gctgataaaa ataaggttag catcgctttt caaggcttcg
      301 gtttcctttt ctatctggtt tagcaacgac aaaggatata ggcttttaac gattaccgcg
      361 aggtcgcgaa attcgcgtat cataggaata atagcgttgt aggcttcggc aatctggtct
      421 tttacgccat caattttatt cttttcattt tcaggctgtg ttttggaata ccacatatct
      481 tcctgtatgg cgctgttgca ataagaattg ggggaggagc cggccaggcc gttttgactg
      541 acttttgtaa aaaaaccggg tatgcctctc attccctggt aaaaactctt tgggttgata
      601 tcattgtctt ctatcaattt taatacggcg ccggcgagtt tccttgcatt attatctgtt
      661 gcggctctcc gggcggagat tccggaaata gcttttgaaa aattggctaa cttaacgtcg
      721 ccaaacaggt ggagaaattt ttgcgtgtcc tctttcaaca gttgtctgga gaatgttttc
      781 aggtcctggc tgatgtccca gttctggcca tcatccattt tttgttcgat aaacccgatg
      841 agcatcctgg taatctggtc atcggagccg gcataggata tcaacacgtc aacagcttcg
      901 gacaacaccc tgtcggaatc gagctctacc tcgaaatttt gcgaaatctt caggtcgtaa
      961 gcaaacgtgc ggattatttt ttgtacaaaa ctgtcaatag tgctgatggc aaaatcagaa
     1021 taattatgaa gaattttatg cagaatactg cctgaccggg cggccaggcc tttttcatcc
     1081 aggcctgagc ttgcgcataa ttcgcttatc agcgccccat ttttttttct gacggatttt
     1141 ttttcgtctt ccgtgacggt atcctcaccc aggcctgaaa gcattttcag acttgacata
     1201 atgcggcttt tcatttcata ggctgcttta ttggtaaagg taacagccag gatatgcctg
     1261 tatttggagg gatctgtcag caccagttta agatattctt ttaccagggt atacgtctta
     1321 cctgagcctg cagaggcttt ataaataata aagttttttg tggaatggac catgatgaat
     1381 taaaaagtaa aaataacgtt tttgccaata cgataaattt tttaacctga acatttcaaa
     1441 aaaatgacct ggcaataatt gcaggaatcc gccgtataaa agtcccgttg ttgcatgtgc
     1501 tgttttgttt tgcatgtagc actaaggaaa cgccaactta caatatattc gggttatatt
     1561 tagtattaag aaatgttaaa aataaaaatt ttttttcatt tcatgatatt tgagtatctt
     1621 tgcaaatatt ttaatacaca ataatttatt acaatgaaaa aaggaacagt aaaattcttc
     1681 aatgaattaa aaggttttgg atttattaaa gataatgaat ccgacaagga atacttcgtg
     1741 catgcctctg gccttatcga caagattaag gaaggtgaca cagtaagctt tgaactcacc
     1801 gaaggaaaaa aaggattaaa tgcggtaaac gtaaaactcg tataaattat cccgattgaa
     1861 gattatcaaa gttaaagagg ctgtctcaaa aaggcagcct ctttttttgt agtgagtcgt
     1921 cctattgtaa tcctgaagat ataaaagact gtcgcgattg tgagacagtc tctctttttt
     1981 gtggcttatg gtaacataga attaataagt ggtaagctgc tggtttataa taaaaaaatt
     2041 ttgcagttat tgtttttatg aatattttta tacctaaatc aaacgatgat ataaatgtcc
     2101 atataattac ttgattatga aaaaactagt ttttttgtta ttctctatgg tcatcagctg
     2161 cctggctaat gggcaaacca ctttgatttc tcctgccggt gatggtggtt ttgaatcagg
     2221 aggaaccccc gcagccaata actggtatgc tgtcaattcc actacagatg gctggtatgt
     2281 aggttctgtg ccggtaccgg caacgggaac caactgtgct tatatttctt ccgcagcggg
     2341 cacttcctgg acttacaacg aaaccagcgc aatacaacat atttactatg atgttattat
     2401 cccggcagga gaatccaaac taactcttac gttcaagtgg aaagccttgg gtgaaggctc
     2461 cggatttagt gactgggata atatgaaagt gttttttggg gcaggttccg ctatg
//
LOCUS       DAOSFP010000348         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_40769_length_2495_cov_3.697131, whole genome shotgun sequence.
ACCESSION   DAOSFP010000348 DAOSFP010000000
VERSION     DAOSFP010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40769_length_2495_cov_3.697131"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLI16_09225"
     CDS             complement(<1..349)
                     /locus_tag="PLI16_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84778.1"
                     /translation="MKLKDLYMIMFLAMLFLPFVIFPALYNFYINANTAHPILLAMLK
                     FGILATTGELIGLRIKTGNYFEKGFGIIPRAVVWAFLGATIAIAFKIFSAGTPACLEY
                     LGVSGVVDAIKGDF"
     gene            complement(393..1295)
                     /gene="xerD"
                     /locus_tag="PLI16_09230"
     CDS             complement(393..1295)
                     /gene="xerD"
                     /locus_tag="PLI16_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOH84779.1"
                     /translation="MKNWSIYLKGFRSFLLLEKSLSVNSIDAYLHDVEKLLQYFEYSQ
                     QAIKPEEVSLQHLQAFLKWINELGMSPYTQARVISGIRAFYKYLIIENIVKNDPAAML
                     ELPKINRKLPDVLSIQEIDALIGAIDLSLPQSERNKAILETLYGCGLRVSELVNLKIS
                     DIYFNEGFVRIIGKGNKERLAPIGTSAMKQIRLYLEHVRVHCEIKKGSADILFLNRRG
                     ARLSRQMVFLMIKGLAEKIGLKKKIGPHTFRHSFATHLIEGGADLRAVQDMLGHESIT
                     TTEIYTHLDREFLRDAIIQFHPRS"
     gene            complement(1319..2038)
                     /locus_tag="PLI16_09235"
     CDS             complement(1319..2038)
                     /locus_tag="PLI16_09235"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOH84780.1"
                     /translation="MIIDLFIPCFIDQVYPQVGANMVKIFEKLGVGVDYNPEQTCCGQ
                     MAFNSGFWDDARRMGEKFIHDFSNNRKIVAPSASCVGMVRNYYSELFNNSSLHNQCKQ
                     IQGNIYEFTDFLVNVLHVEDIGAEFNNVITYHDACAALREYGIKEQPRKLLRHVKGLQ
                     LNEMKDNEVCCGFGGTFSVKFEPISTAMGQQKVQNAIDSGANYIVSTEASCLMHLQGY
                     ISKNKLPMKTMHIVDVLASGW"
     gene            2256..>2495
                     /locus_tag="PLI16_09240"
     CDS             2256..>2495
                     /locus_tag="PLI16_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOH84781.1"
                     /translation="MPQRYFIKLAYDGSRYHGWQQQHNALSIEHLVNEALSTVLRQAV
                     KVTGCGRTDAGVHAAEFYAHFDCNSTIENKETTAYN"
BASE COUNT          718 a          570 c          513 g          694 t
ORIGIN      
        1 aaaaatcgcc ttttatagca tcaacaacac ccgaaacgcc aagatattca aggcaagccg
       61 gtgttcccgc cgaaaaaatt ttgaaggcga tggcaatagt ggcgccaagg aaggcccaca
      121 ccaccgcacg cggtatgata ccgaaacctt tttcaaaata attgccggtc ttgatgcgca
      181 ggccgattag ttcgcccgtg gtagccagga tgccaaattt cagcatggca agcagtatgg
      241 gatgcgccgt atttgcatta atataaaaat tgtacaacgc aggaaatatc acgaatggca
      301 aaaacaacat tgccagaaac attatcatat acaggtcttt gagtttcata gttttttttg
      361 gctaaagtac aaaaatatta tctcctaaaa atttatgatc gggggtgaaa ctgaattatg
      421 gcatcgcgta aaaactccct gtcgagatga gtatatattt cggttgtggt aatggattcg
      481 tgaccaagca tatcctggac ggcgcgcagg tcggcgcccc cttctatcag gtgtgtggca
      541 aaagaatgtc tgaaagtatg agggcctatt tttttcttca ggcctatctt ctccgccagg
      601 cctttgatca tcagaaacac catctgccgg ctcaacctgg cgcccctcct gtttaaaaac
      661 aaaatgtcgg cactgccttt ttttatctca caatgcacac gaacatgttc caggtacaaa
      721 cgaatctgct tcatggctga tgtacctatg ggagccagcc tctctttgtt gcctttgccg
      781 atgatacgca cgaaaccctc attaaaataa atatcggaaa ttttaaggtt taccagctcc
      841 gacacccgca agccacatcc atagagggtt tccagtatgg ccttatttct ttcgctctgc
      901 ggcagactca ggtctatggc acctatcagt gcgtcgatct cctgaatgct gagcacatcg
      961 ggaagcttgc ggtttatttt gggcaattcc agcatggcag ctggatcgtt ttttacaata
     1021 ttttctatga taaggtattt gtaaaaagcc ctgatgcccg aaatcacacg ggcctgggtg
     1081 taaggactca tccccagttc gttgatccat tttaaaaaag cctgaagatg ttgcaggctt
     1141 acttcttcag gttttattgc ctgctgtgaa tattcaaaat actgaagcag cttttctaca
     1201 tcgtgcagat aagcatcaat tgaattaacc gagagtgatt tttcgagtaa taaaaacgac
     1261 cggaaccctt ttaaataaat actccagttt ttcatttatc gataataata agaaaatcct
     1321 accagccgct ggccagcaca tccacaatat gcatggtttt catcggcagt ttgttctttg
     1381 agatataccc ttgcaggtgc atcagacagg aggcctccgt tgaaacgata tagtttgccc
     1441 cgctatcgat ggcattttgc accttttgtt gccccatggc cgttgaaatc ggctcaaatt
     1501 tcaccgaaaa agtgccgccg aaaccgcaac aaacctcatt atctttcatt tcattcaatt
     1561 gcaggccttt cacgtggcgc aacaatttgc ggggttgttc cttgatgccg tattcgcgca
     1621 aggcagcaca ggcatcgtga taggtaatca cattgttaaa ttcagcgcca atgtcttcca
     1681 catggagcac attgacaaga aaatcggtaa attcataaat attaccttgt atttgcttac
     1741 attgattgtg caaagaggaa ttattaaaca actcagaata ataatttcgc accatgccca
     1801 cacacgatgc cgatggggca acaattttcc ggttgttgga gaaatcatgg ataaacttct
     1861 cccccatgcg gcgggcatca tcccaaaagc cgctgttgaa agccatctgc ccgcaacagg
     1921 tttgttcggg attataatca acaccaacgc caagtttttc aaatatttta accatgttgg
     1981 cacccacctg tggataaacc tggtcaatga aacatggaat aaacaggtca ataatcatgt
     2041 aataatatct ttaatgaaag caaatgtaaa aataaatttt gttttttagt acttttggat
     2101 aattaatcat atgctgatta ttttgatcac atgaagtatt ataccatttt tcagcaaatg
     2161 tataaaaacc accatgcacc gcaagggatt ttttctttga ttacggcata ttgccataac
     2221 gccgccttta ttaacctgtg cttatattag acaacatgcc gcagcgctat ttcatcaaac
     2281 ttgcctacga cggaagccgc taccatggct ggcagcagca gcacaatgcc ctgagcatcg
     2341 agcatctggt caatgaagct ttgagcacgg tgctgcggca ggctgtaaaa gttaccggtt
     2401 gcgggcgtac cgatgcaggg gtacatgccg ctgagtttta tgcacatttt gattgcaaca
     2461 gcactatcga aaacaaggaa acaacggcat ataat
//
LOCUS       DAOSFP010000349         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_41368_length_2471_cov_2.652732, whole genome shotgun sequence.
ACCESSION   DAOSFP010000349 DAOSFP010000000
VERSION     DAOSFP010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41368_length_2471_cov_2.652732"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLI16_09245"
     CDS             <1..769
                     /locus_tag="PLI16_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84782.1"
                     /translation="YKKQNPHLLVVNSIIFLCCTSGIILWFRVFDAHDYYLVNYLIFI
                     PATCLTFFRFLQTEFPHWLKSLKLKIPVLGVVLLSICWGHAVVEARYANADTPEQDYS
                     PLLSRYEVDNYRYAHWMYPQEKQAFETITPYLRSIGITSDDKVFSFPDPSPNISLYLM
                     GQRGIALQRDNGVELLKYLDFYKDLHIKYFILSDSTLLQDETTKKILSRKIGQYKTVS
                     IYDISEWDSIKDDTKSIGESLLPAETGITADARARRK"
     gene            783..2144
                     /locus_tag="PLI16_09250"
     CDS             783..2144
                     /locus_tag="PLI16_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84783.1"
                     /translation="MEKESNSKYNTQTLFSRVIKKYAAAITGVFFILMSLFIVNNIPV
                     KYSRMIWSDAEAYYDYLPYTFIKKDIAHMHYAVPLPGGNRLNKVTMGVAMMELPFFLA
                     AHAYSKIQDLGADGYSFPYQVALLISISVYMFLGLFVLFKILRPRFEKKTAFFTVAAL
                     FFASNLYYYTFCEPGLSHGYSFFMLTLFLFRLDYFIKNPNIKNTLLCGIPLAWATLIR
                     PTNILYVILFLLYEVYSVRQLKERLSFIGKNLRYFFIFLILGILFYIPQIMYWSYVWG
                     KLKIYGYVAADMQPETFAYLTSPKIFKVLFGVESGWLVYTPFFIFFFIGLIITLVKKL
                     HHSFAILIIFLLILYLNASWWSYTFACSFGYRSLIEFYPLFAFPVAFLFSRIFASRHI
                     ILKTTMMLLIVVFSFFNIRISGFYYKDPCWEVPQWTWNHYRKIIIKAFYIQPDLKLKE
                     WRE"
     gene            2201..>2471
                     /locus_tag="PLI16_09255"
     CDS             2201..>2471
                     /locus_tag="PLI16_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84784.1"
                     /translation="MKLRAKFLFSALLCCSILLFAQCKKDETEEIPVDELAFTEAEKN
                     LIYSGDNDSIMRIMNYFVHEDSLILRKQSIDVNLNDTAILNRLIKR"
BASE COUNT          692 a          519 c          468 g          792 t
ORIGIN      
        1 ttataaaaag caaaatccac acctgctggt tgtcaacagc attatttttc tttgttgtac
       61 aagtggaatc atattgtggt tccgggtttt tgatgcacat gactattacc tggtaaatta
      121 tctgattttc atcccggcaa cctgtttgac atttttcagg tttttgcaga cagagtttcc
      181 gcattggtta aaatcactga aactgaagat cccggtgtta ggagtagtct tgttgagtat
      241 ctgctggggg catgctgtag tggaggcccg ttatgcaaac gcagacaccc ccgaacagga
      301 ctattcgcct ctgctatcca gatatgaggt tgacaattac cgctatgcac actggatgta
      361 cccgcaggaa aaacaggcct ttgaaacaat tactccctac cttcgctcca ttggtattac
      421 ttcggacgat aaggttttct cgttccccga tccatcgcct aatatatcct tatatctcat
      481 gggacagcgg ggtatagccc tgcaacgtga taatggagtg gagctgttga aataccttga
      541 cttttacaag gacttacaca taaaatattt tattcttagc gattcaaccc tattgcagga
      601 cgaaactaca aagaaaattc tctcgagaaa gatcggccaa tataaaacgg tttctattta
      661 tgacatcagc gaatgggact caattaagga cgatacaaaa tccatcggcg aaagcctgtt
      721 gcctgccgag accggaataa cagccgatgc ccgggcacgc aggaaatgat taattctaaa
      781 ttatggaaaa agagagtaat tccaaataca acacgcagac attattttca agggttataa
      841 agaaatatgc cgcggcaatc acaggagttt tttttatttt aatgagcctg ttcatcgtta
      901 acaatattcc ggtcaaatac tcacggatga tatggtcgga tgctgaggcc tattatgatt
      961 accttcctta cacatttata aaaaaagaca ttgcacacat gcattatgcc gtgccacttc
     1021 ccggtggaaa ccgcctgaac aaagttacca tgggggttgc catgatggaa ttaccatttt
     1081 ttttagccgc acatgcttat agcaaaatcc aggaccttgg cgccgacggg tattcctttc
     1141 cttatcaggt ggccttgctg atttcaataa gtgtgtacat gtttttgggg ttattcgtct
     1201 tattcaaaat tctcaggcca cgcttcgaaa aaaaaaccgc tttttttacg gtagcagcct
     1261 tgttttttgc cagcaacctt tattactaca cattttgcga gccaggctta tcgcatgggt
     1321 attcattttt tatgctgaca ctgtttttat ttcgacttga ctatttcata aaaaatccaa
     1381 acatcaagaa tacactcctt tgcggcatcc cgcttgcctg ggcaaccctt atcaggccta
     1441 ctaacatact ttatgtgatt ctgtttttgc tgtatgaggt atattcggtt cgtcagttaa
     1501 aagaaagatt gtcttttatc ggcaaaaatc tccgttactt tttcatattc cttattctcg
     1561 gaatcttgtt ttatatccct caaataatgt actggtctta tgtatggggg aaactgaaga
     1621 tatacggtta tgtagctgcc gacatgcaac ccgaaacctt tgcttacctt acctctccta
     1681 aaattttcaa ggtgctcttc ggtgtggaaa gcggctggct ggtatatacg cctttcttta
     1741 ttttcttttt tataggattg attatcacac tggtaaaaaa actacatcat tcgttcgcta
     1801 tcctgatcat ttttctgctc attctttatc tcaatgccag ttggtggagc tatacctttg
     1861 cctgttcgtt cggataccgc agccttattg agttttatcc tctctttgct tttcctgtgg
     1921 cctttttgtt ctccagaata tttgcttcac ggcacatcat cctaaaaaca acgatgatgc
     1981 tgctaatagt agttttttca tttttcaata tccgaatttc gggattttat tacaaagacc
     2041 cttgttggga agtgccgcaa tggacctgga atcattaccg gaaaattatc atcaaggcct
     2101 tttacatcca accggattta aaacttaaag aatggcgcga ataaaatgca acactattgt
     2161 ttttttaata aatttgccat aaaaaataat ttttcaagtc atgaagctcc gagcaaaatt
     2221 tcttttttca gcgctgttgt gttgtagcat cctgttattt gcccaatgta aaaaagatga
     2281 aacagaagag ataccggttg acgaactggc attcaccgaa gctgaaaaaa acctgatata
     2341 cagtggagat aacgactcca taatgcgtat catgaattat tttgtgcatg aagattcgct
     2401 gattttacgc aaacaatcta tcgatgtaaa tctgaatgat actgccatcc tgaatcgcct
     2461 tatcaaaaga a
//
LOCUS       DAOSFP010000350         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_41746_length_2455_cov_2.982500, whole genome shotgun sequence.
ACCESSION   DAOSFP010000350 DAOSFP010000000
VERSION     DAOSFP010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41746_length_2455_cov_2.982500"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PLI16_09260"
     CDS             complement(<1..866)
                     /locus_tag="PLI16_09260"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOH84785.1"
                     /translation="MKIIILGAGLVGSPMAIDLSKDEDTEVFVADISPVMFEYLHSFG
                     IKTIQQDLSLPETVKNIVSGYDMVINAVPGFMGFQTLKAIIEAGKNVVDISFFPEDPF
                     LLSELALKNNVIAVMDCGVAPGMMHILTGYAHHQLDETETCLTYVGGLPQIREYPYEY
                     KAVFSPIDVIEEYTRPARYIENYKAVTKEALSDAELLNFPGLGTLEAFNSDGIRSLGS
                     TIKAANIKEKTLRYPGHIGLMKVLRQTGFFSQETIDVKGTKIKPMDVTAKLLFPMWEL
                     KKGEKDLTIMKV"
     gene            999..>2455
                     /locus_tag="PLI16_09265"
     CDS             999..>2455
                     /locus_tag="PLI16_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOH84786.1"
                     /translation="MKNVISCFLILFSLNLFSQGYWTQKADFGGTPKYWAVGFSINGK
                     GYAGLGRGLGFTNSSDLWEYNPVTNAWTQKTSFPGGARYSSHVFVINNKVYFCSGAYW
                     SGISGDYTPYNDVWEYDPAIDSWAQKNNFPGPGRHDAFAFSFGGSGYLCFGQDINGNR
                     LNDLWEYNPTMDTWTQKASFPGTARSGGIQFGFGKYGYIGLGRDNNNVSLGDIWRYDC
                     SLNTWTQMGNFPGTSRTSASTFVTDFRAYVVCGYNFQSASYTTQLWEYNPVADSWVQR
                     EDFPPASGRACGTPFAINNRGYFGLGNVNNTFYKDLWEYTPSCLVGINTNVTVVGSTI
                     YAVAAGASYQWLDCNNAYTVLPGETHQSYSPAVSGNYAVIVTQSSCSDTSACTAVDIC
                     DSINTNVTVIGLTIYSLAAGATYQWLNCDVGMAVIDNETNQSYSPPVNGNYAVVVTQG
                     NCSDTSDCVAFSNIGIDEQGNSGINIYFDPLHNEI"
BASE COUNT          647 a          509 c          565 g          734 t
ORIGIN      
        1 atgactttca tgatagtaag gtctttttcg ccttttttca attcccacat ggggaacagc
       61 agttttgccg ttacatccat gggtttgatt tttgttcctt taacatcgat ggtttcctgg
      121 ctgaaaaatc ctgtttgccg caacactttc atcagcccga tgtgtcccgg ataccgcagt
      181 gtcttctctt tgatattggc tgctttgatc gtgctgccca gcgaacgtat gccatcgctg
      241 ttgaacgcct ccagcgtgcc gaggcccgga aaattcagca attcggcatc cgacaaggct
      301 tctttggtta ccgctttgta gttttctatg taccgcgcag gcctggtgta ttcttctatg
      361 acgtcaatgg gtgaaaacac cgctttgtat tcgtagggat attcgcgaat ttgcggtagc
      421 ccgccgacat aggtaaggca ggtttctgtt tcatcgagct ggtggtgggc gtacccagtg
      481 agaatgtgca tcatgcccgg cgccacaccg caatccatca ctgcgataac attgtttttc
      541 agggcaagtt cactcaacaa aaacgggtct tccgggaaga aagagatatc caccacattt
      601 tttccggctt cgataatggc cttcagggtt tgaaacccca taaatccggg gactgcgttg
      661 attaccatat cataacctga aacaatattc tttaccgttt cgggcaacga aagatcctgc
      721 tgaatagttt taattccaaa ggaatgtaaa tactcaaaca taacgggact gatgtctgcc
      781 acgaacactt cggtgtcttc atctttggaa aggtcgatag ccattgggct tccaaccagg
      841 ccggcgccga gaataatgat tttcatagtt agtttttagg acaaatctac aaaaaactct
      901 tcatatgcta atccgctatt tattggtatt attgaaaaat tggaaactat cttactaagt
      961 ttaaaaaaaa ctgtatattt accgaaaaac atatagatat gaaaaatgtt atttcctgct
     1021 ttttgattct tttttcctta aatctatttt cgcaggggta ttggacccag aaggcagatt
     1081 ttggaggcac ccccaaatat tgggctgttg gtttttccat caatggaaag ggctatgccg
     1141 gactggggcg tggattaggt ttcactaatt caagcgacct ctgggagtat aaccccgtca
     1201 ctaacgcctg gactcagaaa acgagctttc caggaggggc taggtattca tcgcatgttt
     1261 ttgtaattaa taataaggtg tatttttgtt ctggtgccta ctggagtggt atctcaggcg
     1321 attatacacc gtataacgat gtctgggaat atgaccctgc catagattca tgggctcaga
     1381 aaaacaattt tccaggcccg ggcaggcatg atgcgtttgc tttttctttt gggggctcgg
     1441 ggtatttatg ctttgggcag gatattaacg gcaaccgttt aaatgattta tgggagtata
     1501 atccaactat ggacacctgg acacaaaaag ccagctttcc tggcactgcc cggtcaggag
     1561 gtattcagtt tggttttgga aaatatggct atattggtct tggaagggac aacaataatg
     1621 tgtcattagg tgatatatgg cgttatgatt gttctctaaa tacctggacc cagatgggta
     1681 attttccagg cacaagcaga acttcggcat caacatttgt aactgatttc agagcgtatg
     1741 tggtttgcgg atataatttc cagtcagcgt cctacacaac ccaattatgg gagtataacc
     1801 ctgttgccga ttcatgggtg caaagggagg attttccacc tgcaagcgga agagcttgcg
     1861 gaactccttt tgctattaac aacaggggct attttggatt aggaaatgtg aataacacat
     1921 tttataagga tttgtgggaa tatacaccat cgtgcctggt gggcataaac acaaacgtta
     1981 ccgttgtcgg atcaacgata tatgccgtag cagcaggtgc ctcataccag tggctggatt
     2041 gcaataatgc ttatactgtt ttacctggcg aaacccacca aagttattcg cctgcggtaa
     2101 gcggaaatta tgccgtgatc gtgactcaaa gttcatgtag cgatacatca gcttgtaccg
     2161 cagtagacat ttgtgattcg ataaatacaa atgttacggt aatagggctg actatctatt
     2221 cactcgccgc cggcgctact tatcaatggt tgaattgcga tgtcggcatg gcggttattg
     2281 ataatgaaac gaatcaaagt tattcgcccc ctgtcaatgg aaactacgct gttgtggtaa
     2341 cgcagggcaa ctgcagcgat acttccgatt gtgttgcttt ttcaaatatt ggtattgatg
     2401 aacagggtaa ttcaggcata aacatttatt ttgatccact gcataatgaa ataaa
//
LOCUS       DAOSFP010000351         2446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_41960_length_2446_cov_3.759515, whole genome shotgun sequence.
ACCESSION   DAOSFP010000351 DAOSFP010000000
VERSION     DAOSFP010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41960_length_2446_cov_3.759515"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..1025)
                     /locus_tag="PLI16_09270"
     CDS             complement(207..1025)
                     /locus_tag="PLI16_09270"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOH84787.1"
                     /translation="MIIDGHAHAAGVFYEKDSLVAELDRLGVDKIVLAPMGVRQDPAY
                     SKENMDTVFANTNINNVEQKINEFVSGLSNIIYDAPNKYLFSLKQQCPERIIQYYWAN
                     PNDPHVMEYLEKYVHLWNFSGVKLHQMVADFKTDQESIHQIAEFCEAHDMPLFIHLFK
                     QKNVFRFMDVCTEHQHTRFIVGHMIGYDILQEHALGLDNFWFDISPSWLVPKKILKKA
                     LAYYGADRLMLGSDTPLGSNKNLEKNLKRVRALAISDSDKDKILGLNLQALLNL"
     gene            complement(1072..1623)
                     /locus_tag="PLI16_09275"
     CDS             complement(1072..1623)
                     /locus_tag="PLI16_09275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOH84788.1"
                     /translation="MKKNCPANSRVLFVFFGLAWLAVMNFSCGNTEKTKVVINEDTIK
                     TSLVKVNKELTAREDRDIENYIQRHEWAMQKTGTGLRYQIYKKGAGAQPKDQDKVVIS
                     FVVSLINGVECYNSKNDGMKLFELGKAQVESGLEEGIKLMHAGDKAKLIIPSHLAFGF
                     MGDDNKIPKRATLIYDVELVEIK"
     gene            complement(1598..>2446)
                     /locus_tag="PLI16_09280"
     CDS             complement(1598..>2446)
                     /locus_tag="PLI16_09280"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH84789.1"
                     /translation="FPGVYKWMPGAGSIKIGTKSKEECTELLLNADLIFCLDFNSPDR
                     VGGLAPALKSSKGEKILIDHHLFPSDFFKITYSRPETSSTTELIYEFVAGLGDESLID
                     YDIAVAVYVGIITDTGSFSHSSSYPGTYVITSKLIEIGIDPAEIQRKVFCNFSESRVR
                     LLGYCLSEKLTIIDKYATAYIVIDKEDMKRFHFKIGDSEGIVNYGLTIKNIRLAALFT
                     EKNGMVKLSLRSTGELDVDKFARKHFSGAGHLRASGADIKKSLKESVEEFISLLPLYE
                     KELSGK"
BASE COUNT          671 a          573 c          484 g          718 t
ORIGIN      
        1 aatgtccatt agagaacatc gtctaaaaaa aaccattaaa aatgtccatt actcctaaaa
       61 gttagatgtt ttattctact ttttaaagtc ttgaaatttg ggttaaaata tatcaaactc
      121 gtgaaactat tgaaaatatt acatccctga ccggatttga tctatctggc ttacttatca
      181 ttctatgaac agcctatcca ggacaatcaa agattaagca gcgcctgcag gtttaatccg
      241 agaattttgt ctttatctga atcggaaatt gccaaagccc taaccctttt aaggtttttc
      301 tccaaatttt tgttactgcc caggggtgta tcagagccga gcatgaggcg gtctgcgcca
      361 taataggcaa gcgctttttt gagaattttt ttaggaacca gccacgacgg agaaatatca
      421 aaccagaaat tgtcaaggcc cagggcatgt tcctgcaaaa tgtcataacc aatcatatgc
      481 cccacaataa agcgtgtatg ctggtgttcg gtgcatacgt ccataaaacg gaagacattt
      541 ttttgcttga acaaatgaat gaaaagcggc atatcatgcg cctcgcagaa ctctgcaatc
      601 tgatgaatgc tctcctgatc ggtcttaaaa tcagcgacca tctggtgcag ctttacaccg
      661 ctgaagttcc acaggtgtac gtatttttca agatactcca tgacatgcgg atcgttgggg
      721 tttgcccagt aatactggat gatccgttca ggacattgtt gtttcaatga aaacaaatac
      781 ttgttaggtg catcgtaaat aatattagaa aggccgctta caaattcatt gatcttttgc
      841 tctacattgt tgatattcgt atttgcaaaa acagtgtcca tattttcttt ggaataagca
      901 ggatcttgtc ttacacccat aggcgccagt acaatcttat ccaccccaag gcggtcgagc
      961 tcagctacca gggaatcctt ctcatagaag actcccgcag catgtgcgtg tccatcaatg
     1021 atcataggtt tttttggtaa atataagcta ttataatccg gaagtcaaca actattttat
     1081 ctccaccaac tccacatcat agatcagcgt ggcacgtttg ggaatcttgt tgtcatcgcc
     1141 cataaagcca aaagccaaat gagagggaat aataagtttg gccttgtcac ctgcatgcat
     1201 cagcttgatg ccctcttcga ggccgctctc tacctgggcc ttacccagct caaacaactt
     1261 catgccgtcg tttttcgagt tgtaacactc cacgccgttg atcagcgaaa ctacaaagct
     1321 gatgactacc ttgtcctggt ctttgggctg cgcccctgct ccttttttat aaatctgata
     1381 tctcaggccg gtgccggttt tctgcatagc ccattcgtgc cgctgaatat aattttctat
     1441 atccctgtct tccctggctg taagttcttt gtttaccttt accaggctgg tttttatggt
     1501 atcttcattt ataaccacct tggtcttttc ggtattacca cacgaaaagt tcatgacggc
     1561 cagccatgcg aggccgaaaa aaacaaataa tacccgacta tttgccggac agttcttttt
     1621 catacaaagg caataaagaa ataaattctt ccacactctc tttcaatgac tttttgatgt
     1681 cagccccgga agctctcagg tggcctgcac cggagaaatg tttgcgggca aacttatcca
     1741 catccagttc accggttgaa cgtaaggaaa gctttaccat gccattcttt tcggtaaaca
     1801 aggcggccag acggatattt tttatggtaa gcccataatt gacaatacct tcactatcgc
     1861 caatcttaaa atgaaaccgt ttcatatcct ctttatcaat aacgatatag gcagtggcgt
     1921 atttgtcaat gatggtaagt ttttcgctca ggcaatatcc cagtagcctc acacggcttt
     1981 ccgaaaaatt gcaaaacacc ttgcgttgta tctccgcggg atctattcca atttcaatta
     2041 gtttcgaggt gatgacgtat gttcccggat agctgctgct gtggctgaac gatccggtat
     2101 cggtgatgat gcctacgtac acggcaaccg caatgtcata gtcgataagc gattcatcgc
     2161 ccaggcccgc aacaaactca tatatcagtt cggtggttga cgaagtctct ggcctggaat
     2221 aggtgatttt gaaaaaatcc gatggaaata aatgatggtc aatcagtatc ttttcacctt
     2281 tggacgactt gagagctggc gccaaccctc ccacccggtc aggcgaattg aagtccagac
     2341 aaaagatcag gtcggcatta agcaggagtt ctgtacattc ctccttcgat ttagtgccta
     2401 tttttatgct tccggcaccc ggcatccatt tataaacccc cggaaa
//
LOCUS       DAOSFP010000352         2434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_42244_length_2434_cov_4.968894, whole genome shotgun sequence.
ACCESSION   DAOSFP010000352 DAOSFP010000000
VERSION     DAOSFP010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42244_length_2434_cov_4.968894"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLI16_09285"
     CDS             complement(<1..372)
                     /locus_tag="PLI16_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOH84790.1"
                     /translation="MLVIGIAGGSGSGKTTVVKKIVNSLPASAVTVISQDAYYKNNGH
                     LSAEERSKINFDHPSSIEFNLLIKHLDILKKGKPIEMPIYSYLTCARAKETIPVQPKR
                     VMIVEGILIFTNAKLLERFDVK"
     gene            462..>2434
                     /locus_tag="PLI16_09290"
     CDS             462..>2434
                     /locus_tag="PLI16_09290"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HOH84791.1"
                     /translation="MKKIVLSAFMLLVVCAGFAQEKNPTSPLLYHADDMLLKKAVTEK
                     PELINQYMIYEENLKNLINSIYSQNGKNGAKTDTLIDGKRIIPVVFHIVHNGGPENIS
                     REQIEDAIKLINIDYNKLNADTTLGHTYPPFNALRADCKIEFRLAKVDPNGNCTDGIM
                     RHYDPQTNYGYFKTMTDYCWTPSRYLNVFTVNFIYPEGMSLPDGAFIGGMSPFPPSNT
                     LSSALTGGDTLADGVLIRHDCIGSIGTATAMGGMPINAINRTFTHESGHYFNLYHTFQ
                     SLLAVIGVDGCLDMLGSGDEVADTPPVSSGTQNTSLACYEPGTVNSCTTDSPDLPDMI
                     ENYMDYQWGYCANMFSLGQYARIDATLQTDRLKLWSKENLIYTGVLDTTPVLCAPIAE
                     FFSSSNLVCAGGNIQFTDYSFSGAVQDYLWTFEGGTPATSTDQNPLVVYNTPGTYFVK
                     LSVSNASGADSLIKQDMITVKPATAALLAPFMENFETTVLNNGWIVKNDVGNQWEISD
                     TAYFSASKALRLKNFTGNTAGSFDEIITPAYDLTSLPAGGTPFVRFKLAYAGKISSSD
                     TVYDALKMFVSKDCGETWSEKYTESGATLGSTNATSGSFAPATTADWKEISRPLPGFL
                     TANNVMFKFVFHSNGGNNVYIHDINVTTQIAGC"
BASE COUNT          700 a          522 c          530 g          682 t
ORIGIN      
        1 tttaacatca aaacgttcca gaagtttggc attagtaaaa atcagtatgc cttccacaat
       61 cataacacgc ttaggctgaa caggaatggt ctctttagct cttgcacacg taaggtatga
      121 ataaatgggc atttcgatag gtttgccttt ctttaatatg tcgaggtgtt tgatcaacag
      181 gttgaactcg atggaagagg ggtggtcaaa atttattttt gatcgttctt cggcgctgag
      241 atggccgtta tttttgtaat aagcatcctg tgatataaca gtcacggcac ttgccggcaa
      301 actgtttacg atttttttta ctacggtggt ttttcccgac ccggaaccac cggcaatacc
      361 aatgactagc attttttttt tcaaaattac aaaaaacaga aataggacaa cggcactacc
      421 ctcaaaaaaa aatgtatttt tgtgaaaaaa atttattggt catgaagaaa attgttttat
      481 cagcattcat gctgttggtg gtttgtgcag gatttgccca ggagaaaaat ccgacatctc
      541 cgttacttta tcatgcagat gatatgttat taaaaaaagc ggttaccgaa aagccggagc
      601 taatcaatca atacatgatt tatgaggaaa acctgaagaa cctcattaat tctatatata
      661 gccagaacgg gaaaaatgga gccaaaacag atacactcat tgacggaaaa agaattattc
      721 ccgtggtgtt tcacatcgtg cacaacggcg gccccgaaaa tatcagccgg gaacaaattg
      781 aggatgccat caaacttatc aatattgact ataataaact taatgccgat accactttag
      841 gtcacacata tcctccgttc aatgccttga gagccgactg taagatagag tttcgcctgg
      901 caaaagtcga tcccaacgga aactgtaccg atggtattat gagacattat gatccccaga
      961 ccaactatgg ctattttaaa actatgacag actattgttg gacaccaagc cgttacctga
     1021 atgtttttac tgtgaatttt atttatcccg aaggtatgtc tctgcctgat ggggccttca
     1081 tcggcgggat gtctcctttt cctcccagca ataccttgtc gtcggcactt accggtggcg
     1141 ataccctggc tgacggggtg ctgataaggc acgactgtat cggtagcatt ggcacagcta
     1201 cagctatggg cggaatgcct atcaatgcaa ttaacaggac atttacccat gaaagcgggc
     1261 attatttcaa cttataccac acctttcaat ctcttctggc cgtcataggg gttgatggat
     1321 gccttgatat gctgggaagc ggtgatgaag tggccgacac tcctccggtt tcttcaggca
     1381 cacaaaatac ctctttggcc tgttatgaac cgggcacggt gaattcctgc accaccgaca
     1441 gtcccgacct gccggatatg atagaaaact atatggacta tcagtggggc tattgtgcaa
     1501 atatgttttc attaggacag tatgcaagaa tagatgccac actacaaacc gatagattga
     1561 aattatggtc aaaagaaaat cttatttata ccggagtgtt agataccacg ccggtgctgt
     1621 gtgctccaat agccgagttt ttctcttctt ctaatctggt ttgtgccggc ggaaatattc
     1681 aatttaccga ttattctttt tcaggagcgg tacaggatta cctgtggaca ttcgaaggcg
     1741 gaacacctgc cacatctacc gatcagaatc ccctggttgt atataacacc ccgggaactt
     1801 attttgtgaa actttcggtt agcaatgcca gcggtgcaga ttcactcatc aaacaggaca
     1861 tgatcacggt gaagcccgct acggctgcat tattggcgcc ttttatggaa aactttgaaa
     1921 ccaccgtgct caacaatgga tggatagtga aaaatgatgt gggtaatcag tgggaaataa
     1981 gcgatacggc ctacttctct gcttccaaag cattacgcct gaaaaatttc acgggaaaca
     2041 cagcaggaag ttttgacgag attatcactc cggcttatga cttaacttcg ctccccgcag
     2101 gcggaacacc ttttgtacgg tttaaacttg cctatgccgg caaaatcagc agttcggata
     2161 ccgtgtatga tgctctgaaa atgtttgttt caaaagattg cggcgaaacc tggtcggaaa
     2221 aatacaccga aagcggtgct actttgggca gtaccaatgc tacctcaggt agttttgctc
     2281 cggcaacaac tgctgattgg aaagagattt caaggccgtt gccgggattt ctgacagcca
     2341 ataatgtgat gtttaaattt gtttttcatt ccaacggcgg taataatgtt tatattcacg
     2401 acatcaatgt tacaacccag atagccggtt gcga
//
LOCUS       DAOSFP010000353         2429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_42384_length_2429_cov_3.654170, whole genome shotgun sequence.
ACCESSION   DAOSFP010000353 DAOSFP010000000
VERSION     DAOSFP010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42384_length_2429_cov_3.654170"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>311)
                     /locus_tag="PLI16_09295"
     CDS             complement(<1..>311)
                     /locus_tag="PLI16_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HOH84792.1"
                     /translation="ADYEWQQLNSRIKSLEEAHIFIDDTPALPIFELRAKARRLVAQH
                     NIQMIIIDYLQLMSGTAETKGMREQEISGISRALKGLAKELSIPVIALSQLSREVEKR
                     P"
     assembly_gap    312..321
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<322..1182)
                     /locus_tag="PLI16_09300"
     CDS             complement(<322..1182)
                     /locus_tag="PLI16_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOH84793.1"
                     /translation="MADIVRKRTKAPVTEQSILQYGKIPPQAVELEEVVLGAMMLEKD
                     ALANVIEILKAEVFYKEAHQHIFTAISHLFNSSQPVDILTVTNELKTMAKLDIAGGPY
                     YIAQLTSKVASSANIEYHSRIILQKFIQRELIRISGEISTEAFEDTTDVFELLDKAER
                     NLFDISETNLRRKYSSIQDILKEAIGEIEKAKEAGKNSDFATIGVPTGYTGLDRLTAG
                     WQKSDLIILAGRPSMGKTAFVLSMARNAAIDYGIPVAIFSLEMSAIQLVLRLISMETE
                     LQSDSLRRGKL"
     gene            complement(1262..1969)
                     /locus_tag="PLI16_09305"
     CDS             complement(1262..1969)
                     /locus_tag="PLI16_09305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOH84794.1"
                     /translation="MLKKVNIENILFLDIETVPRWEDFSQMPENFQKLWAHKTQFLSS
                     EKELSPADVYERAGIYAEFGKIICISVAFVRGGGIKVKSFFGDDEKVLLKEFALLLTQ
                     YYNKEQHNLCAHNGKEFDFPYIVRRMLVNGVEVPDILDLAGKKPWEVKLLDTMELWKF
                     GDYKNYTSLELLSALFDIPSPKDDIKGSDVAGVYWKEKDLERIVRYCQKDVVTIVQLF
                     RRYQNTPLIADAFIVTI"
BASE COUNT          700 a          532 c          446 g          741 t
ORIGIN      
        1 ccggacgttt ttccacttca cggctaagct gcgacagcgc gatgaccggg atgctaagct
       61 ctttggccaa tcccttgagc gccctggaaa ttccgctgat ttcctgttcc ctcattcctt
      121 tagtttcagc agttcccgac atcagctgca aatagtcaat gatgatcatc tggatattat
      181 gctgcgcaac aagcctcctg gcctttgccc tgagctcaaa tatcggcaag gcaggtgtgt
      241 cgtcgataaa aatatgtgct tcttcaagac tttttatacg gctattgagt tgctgccact
      301 cataatcggc cnnnnnnnnn ncagttttcc acggcgcagg ctgtccgact gaagttccgt
      361 ttccatcgaa atcagacgca acaccaactg tatggccgac atttcaagcg aaaaaatagc
      421 aacaggaata ccataatcga tggccgcatt acgtgccatg gaaagcacaa aagcggtttt
      481 tcccatggaa ggccttcctg caagaattat cagatcggat ttctgccatc ctgcggtaag
      541 ccggtcaagg ccggtatagc ccgttggcac cccaatagta gcaaaatcac tgtttttgcc
      601 ggcttctttt gccttttcta tttcgccgat ggcctctttt aaaatatcct gaatactgct
      661 gtattttctg cgcaaattgg tctcgctgat atcgaacagg ttgcgttcgg ccttatcaag
      721 cagctcaaat acatcggtgg tatcttcaaa ggcttcggta ctgatctctc ctgatatcct
      781 gatgagttcg cgctgtataa atttctgcaa tattatacgc gaatggtatt caatattggc
      841 tgaagatgcc accttggatg taagctgggc aatataataa gggcctccgg caatatcaag
      901 cttggccatg gttttcaatt cgttggtaac cgtaaggata tctacgggtt gcgacgaatt
      961 aaacagatgg gaaatagccg taaaaatatg ctgatgggct tctttataaa atacttcagc
     1021 tttcaaaatc tcaatgacat tggcaagggc gtctttttcg agcatcatgg cacccaaaac
     1081 cacctcttcg agttcaacgg cctgaggggg aattttaccg tattgtagta tagattgctc
     1141 ggtaaccggc gctttcgtgc gttttcttac tatatctgcc ataacaactt tttattgttc
     1201 aaaaataact gaatgagcaa cgccttgaac aaaacagcag ataaaaattt atcaacaact
     1261 atcaaatcgt tactatgaaa gcatcggcta tcaggggggt attctgatac ctgcgaaaca
     1321 gctgcactat ggtaacgaca tctttctgac agtacctgac aatgcgttcc agatcttttt
     1381 ctttccagta aacgcccgcc acatcgcttc ctttgatatc atcttttggc gaaggaatat
     1441 caaacagggc actcaacaat tccagggatg tatagttttt ataatcaccg aacttccata
     1501 gttccatagt atccaggagt ttaacctccc agggcttttt accggccagg tcgagaatgt
     1561 caggaacctc caccccatta accagcatac gccgcacgat gtatggaaaa tcaaattcct
     1621 taccgttatg tgcacaaagg ttatgttgct ctttattata atactgtgtc aataataaag
     1681 caaattcctt taataatacc ttttcatcat cgccaaaaaa cgacttaacc tttattcctc
     1741 cccctctgac aaatgccacc gatatgcaaa tgatttttcc gaattcggca tagattccag
     1801 cccgttcgta cacatcagca ggcgacaact ctttttcaga agaaagaaat tgggtcttat
     1861 gagcccatag cttttgaaaa ttttcaggca tttgagaaaa gtcttcccaa cggggtactg
     1921 tttcaatatc cagaaacaaa atgttttcaa tatttacttt tttaagcata tgattaaatt
     1981 tatcccatta taattatcaa tctgacaggt attaccgttt cttcgtataa cccggacgct
     2041 ggccaggcag accgaaaagt cataatactt aactcaaagc aatttttcgt ttatctattg
     2101 aaacccaaag gatttcgata cgttttatgc ttagcgaagc ttattattct aatcggtatt
     2161 aatttgtaaa gttaagaatt tttttgtttc aaacacaaca tatatatcat tttctatttt
     2221 cctgataaaa ttcaccacta tttaaattgg tatttacaca gttaaatatt gtgcaaccaa
     2281 tgcgaaattt ataggagaaa ttatatgaat cattctggca ttttgtaaat ttttgattgc
     2341 atttaatcaa gtattcatac aaataatttt ataattaaaa tgaagttggt atgaatgatt
     2401 gaccaaggtt tagcataaat aaacaaaat
//
LOCUS       DAOSFP010000354         2414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_42790_length_2414_cov_3.641797, whole genome shotgun sequence.
ACCESSION   DAOSFP010000354 DAOSFP010000000
VERSION     DAOSFP010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42790_length_2414_cov_3.641797"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1105
                     /locus_tag="PLI16_09310"
     CDS             194..1105
                     /locus_tag="PLI16_09310"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOH84795.1"
                     /translation="MNWKLTILGSNAATPANDRNPSAQVLDCDDRLFLIDCGEGTQMQ
                     LRKYKIKFQRIDHIFISHLHGDHYLGLAGLLFTYHLFGRTKKLHVYANAELKEIIGLQ
                     LRASGTTLLYPLEFHDLPENQAASLFDDAKISIRCFPLLHRVPTHGFVFRQNFTERNI
                     IKEKIDELEIPMRQLAAIKKGADFTDKNGNVHPNAELTFEPPPAKMFAYCSDTGFSPK
                     IVPYIKNASLLYHEATFMQDMQANADAKQHSTTVNAATIAKKAQVNKLLIGHFSARYD
                     ELTDVIEETRSIFPDADLAIEGNVYKI"
     gene            1372..1761
                     /locus_tag="PLI16_09315"
     CDS             1372..1761
                     /locus_tag="PLI16_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84796.1"
                     /translation="MKKNIPLYLLIAFFISACNQTNVLIGNWDITEVTVDTNLIPDKM
                     DVPTATGLFVLGETSKPTSIIITKDSIQLCSNSDRTSDKISKINKTEKNKFLIDFGKK
                     SGIFEIQEGGTAKFTVDAMTYFLKKGK"
     gene            complement(1773..2402)
                     /gene="recR"
                     /locus_tag="PLI16_09320"
     CDS             complement(1773..2402)
                     /gene="recR"
                     /locus_tag="PLI16_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOH84797.1"
                     /translation="MASYPSKLFEQTVNEFAKLPGIGRKSAVRMVLHLLGETSETADA
                     LGNAIVKLRKEVKYCKHCFNITENEVCDICANVKRDKSVICVVEDIRDVIAIENTNQY
                     NGVYHILGGIISPMDGVGPNDLNIKQLLGRIAEGNCSEVILALPPTMEGDTTNFFLYR
                     KLCDFNICISTIARGISFGDELEYADEVTLGRSILNRTPYQTEPQKSNK"
BASE COUNT          719 a          532 c          497 g          666 t
ORIGIN      
        1 ggtcccggaa catgcggtaa aaaaaacaag cgctatcaat gcatacacta tccttactgt
       61 tttcatacgg atgattttat atgcaaattt atatgttttt atccatttac aagaaaccgg
      121 attttttatt gtttgaaaag acagctatta aggccgttaa caacgcacga aaaaaagacg
      181 tacctttatc cgcatgaatt ggaaattgac gatactgggc agcaacgcag ccactcctgc
      241 caatgaccgc aatcccagcg cacaggtgct cgactgcgac gaccgccttt ttctgatcga
      301 ctgcggtgaa ggcacgcaga tgcagttgag gaaatacaaa ataaaatttc agcgcatcga
      361 ccatattttt atttcgcacc tgcacggcga ccattacctg ggccttgccg gactgctgtt
      421 cacctatcac ctgttcgggc gtaccaaaaa actacatgtg tatgccaatg cagaacttaa
      481 agaaattatc ggtttgcagt taagagcttc tggtactaca ctcctttacc cgcttgaatt
      541 tcacgacttg ccggaaaacc aggcagccag cctttttgat gatgcaaaaa ttagtatccg
      601 ttgctttccg ctgttgcacc gggtgcccac gcacgggttt gttttcaggc agaatttcac
      661 cgaacgcaac attatcaagg aaaagatcga cgaacttgaa atccccatga ggcaattggc
      721 agccatcaaa aaaggtgctg attttacaga taaaaacgga aatgtacatc ctaacgccga
      781 acttactttt gagccgccgc cggcaaaaat gtttgcctac tgctcggata ccggcttcag
      841 cccgaaaata gtaccctaca taaaaaatgc ttcgctgctg tatcacgaag ccacctttat
      901 gcaggatatg caggccaatg ccgatgcaaa acaacattcc accaccgtaa atgccgctac
      961 catagcaaaa aaagcccagg tcaacaaatt gcttatcggg catttttcag ctcgttatga
     1021 tgaactaaca gatgttattg aagaaacacg cagcatattt cctgatgccg atctggctat
     1081 cgaaggaaat gtatataaga tttaggccaa aagagttgag gcagtttcaa aagcactctg
     1141 tgtaactctg tgcaactctg tgtatactcc gtggcactct gtgtaattgt ttattttatt
     1201 gtcacacaga tattcacaga gaccgctacc gcggctcaca gagagtcact gagtgaaaat
     1261 acagctatta aaaataatat caaccaattc atacaatttc taaaataaat tctttatatt
     1321 tatagggtga ttagttattt catttatact aacccataaa aactcactaa gatgaaaaag
     1381 aatataccgc tttatttgtt gattgcattt tttatcagcg cctgtaatca aacaaatgta
     1441 ttgattggta attgggacat taccgaagtt actgttgaca ctaatttaat accggacaaa
     1501 atggatgtgc caacagctac aggcctcttc gtgttgggtg aaacttcaaa gccgacaagt
     1561 ataattatta caaaggactc cattcagcta tgtagtaatt cagacaggac atctgataaa
     1621 atatctaaaa ttaataaaac tgaaaaaaat aaattcctaa ttgattttgg taagaaatcc
     1681 gggatttttg aaattcagga aggtggcacg gcgaaattca cggttgatgc tatgacctat
     1741 tttttgaaga agggcaaata acagtataaa aactacttgt tgcttttctg gggttccgtc
     1801 tggtacggtg tccggttcag gatggaacgc cccagtgtaa cttcgtcggc atactccagc
     1861 tcgtcgccaa aagaaatgcc gcgggcaatg gttgagatac agatattaaa atcgcataat
     1921 ttccggtaaa ggaagaaatt ggtggtatcg ccttccatgg ttggcggcaa ggcaaggata
     1981 acctccgagc agttgccttc ggcgatacgg cctagcaatt gctttatgtt gagatcgttg
     2041 gggccaacgc catccatagg cgaaataatg cctccaagaa tatggtacac cccgttgtac
     2101 tggttggtgt tttcaatggc aataacatcg cgtatatcct ccaccacgca gatgaccgat
     2161 ttgtcgcgtt ttacattggc gcagatgtcg caaacttcat tttcggtaat attgaaacaa
     2221 tgtttgcagt actttacttc cttacgcagt ttgacgatgg cattacccag cgcatcggcc
     2281 gtttcgcttg tttctcccaa caggtgcaac accatgcgca ccgctgattt gcggcctatg
     2341 cccggcagtt tggcaaattc attgaccgtt tgttcgaaaa gttttgaagg atagcttgcc
     2401 accggaattt tttt
//
LOCUS       DAOSFP010000355         2413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_42821_length_2413_cov_3.268872, whole genome shotgun sequence.
ACCESSION   DAOSFP010000355 DAOSFP010000000
VERSION     DAOSFP010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42821_length_2413_cov_3.268872"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLI16_09325"
     CDS             <1..617
                     /locus_tag="PLI16_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH84798.1"
                     /translation="VVLTATSVHGCHAEGRDTIIVYQTPEAGFYPHPDIARLDEPEIF
                     FYDQSNNAQHWYWDFGDPASIDSNYSFDHNTSHHYSDTGTYLVMHVVTNIYDCADTVY
                     QTVKIINPFYFWVPGAFTPNGDATNNVFMGQGVGFREEDFSMVIYDRWGELVFKTNDP
                     YQPWDGIKAGANIKCPAGVYVWMIDITEENTVHHKMKGTVTLIR"
     gene            complement(667..1386)
                     /locus_tag="PLI16_09330"
     CDS             complement(667..1386)
                     /locus_tag="PLI16_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH84799.1"
                     /translation="MKKLSVLIPAYNEEKTISAVLDKVLSVSLINNITKEIIVINDCS
                     RDGTENQILEYIKSHHEADIQYVKHEINQGKGAAIHSGIAKASGDYIIIQDADLECDP
                     EEFNLLLKPMDEGFADAVFGSRFAGGNIHRVLRKRNLRANKFLTWLTNLFIDLTLTDM
                     ETCYKLFRSEIIKNIHLTEKRFGFEPEVTIKVARVPRIKIYELSISYYARGVDDGKKI
                     GWKDGLRAIYCILKYGLFRAK"
     gene            complement(1393..2406)
                     /locus_tag="PLI16_09335"
     CDS             complement(1393..2406)
                     /locus_tag="PLI16_09335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH84800.1"
                     /translation="MKAAVVILNWNGKKFLEQFLPAVIQHSKNIASIIVADNASSDDS
                     VAFLKEHFPEIRIIQNQSNGGFAKGYNDALKLIDAEYYVLLNSDIEVTPGWIEPVLGL
                     MDSDKSIAACQPKIRSYHEREKFEYAGAAGGYIDKYGYPFCRGRLFFTIEEDEGQYND
                     TREIFWATGACLFVRAELYHKLGGLDEDFFAHMEEIDFCWRLKNHGYKIMYCPDSIVF
                     HIGGGTLPKSSSRKTYLNFRNNFTLLYKNLPAKRLAPVFTARLLLDGIAAIKFLLDGG
                     FKDFIAVSRAHLSFYRNIGKIRKKRKLQIQQKVRHIYHGNIVVDYYLLKRKKFTDLKK
                     KIY"
BASE COUNT          655 a          479 c          507 g          772 t
ORIGIN      
        1 tcgtggtgtt aacggctact tcagttcatg gttgtcatgc tgaaggaaga gatacaatta
       61 tagtctatca gacgcctgaa gcggggtttt atccacaccc cgatattgcc aggctggatg
      121 agccggaaat ctttttctac gatcaatcca ataatgcgca gcactggtat tgggattttg
      181 gcgaccccgc atccattgac agtaattact cttttgacca taatacttcg caccattata
      241 gcgatacagg aacttacctg gtaatgcatg tagtaaccaa tatttatgat tgtgctgata
      301 cggtttacca aacagttaag attatcaatc ctttttattt ttgggtgccc ggcgccttta
      361 cgccaaacgg cgatgctaca aataatgtgt ttatgggaca gggtgttggt ttccgggagg
      421 aagatttttc aatggtcatt tatgaccgtt ggggtgagtt ggtattcaaa accaacgatc
      481 cttatcagcc atgggatggt atcaaggcag gtgcgaacat caaatgccct gcaggtgttt
      541 atgtgtggat gattgatatc acggaagaaa ataccgtaca tcataaaatg aaaggcactg
      601 taacactcat aagatagaga aggtaaaacg ggaaagcggt tttataccgt gggaaattgt
      661 atgtgattat tttgctctga ataacccata tttcaggatg cagtaaatgg ccctgagacc
      721 atctttccat cctatctttt tgccatcatc cacgccacgt gcgtaataag aaatgctgag
      781 ttcgtaaatt tttattcggg ggaccctggc cacttttata gtaacttcag gttcaaaacc
      841 gaaacgtttt tcggtgagat gaatgttttt tatgatttct gacctgaaga gtttgtaaca
      901 ggtttccata tctgtcagcg taagatcaat aaacaggttg gtcagccagg tgagaaattt
      961 gttggctctc aggttgcgtt ttcttaatac cctgtggatg tttcctccgg caaagcgcga
     1021 gccgaatacc gcatcggcaa agccttcgtc catgggtttt aataataggt tgaattcctc
     1081 cggatcgcat tcgaggtcgg cgtcctgaat gatgatatag tcgccggaag ctttagctat
     1141 tcccgaatgt atggcggctc ctttaccctg gtttatttca tgctttacat attgaatgtc
     1201 ggcctcatga tggcttttta tatattcaag tatttgattt tctgtgccat ctctggaaca
     1261 atcgttgatt acaatgattt cttttgttat attattaatt aaactaacac tgagtacttt
     1321 gtccagcaca gcgctgatgg ttttttcttc gttataggca ggaatcagga cagatagttt
     1381 tttcataaag ggttaatata ttttcttttt caggtccgta aatttttttc ttttcaacag
     1441 gtaataatca acaacaatat taccatgata tatgtgtctg actttttgtt gtatttgcaa
     1501 ttttcttttt tttcttattt ttccaatatt tctgtaaaaa gacagatgag cgcgtgacac
     1561 agcaatgaaa tctttgaatc cgccgtcaag aagaaattta atggcggcaa taccatcaag
     1621 taaaagccgg gcggtaaaaa cgggcgctaa cctttttgca ggaaggtttt tgtatagcag
     1681 tgtaaaatta ttcctgaaat tcaggtaggt ttttcttgac gaactttttg gcagggttcc
     1741 accaccgata tgaaaaacga tggagtctgg gcagtacatg attttatatc catggttttt
     1801 taaccgccag caaaaatcta tttcttccat gtgggcaaaa aaatcttcat caaggccgcc
     1861 taatttatga taaagttccg ccctgacaaa gagacaggct ccggtagccc aaaatatttc
     1921 cctggtatca ttatactgtc cctcgtcttc ttcaatcgta aagaacaatc ttccacgaca
     1981 gaaaggatag ccgtatttgt cgatatatcc gccggctgca cctgcatatt caaatttttc
     2041 tctttcgtgg tacgatcgga ttttgggctg acaggcagca atacttttat cagaatccat
     2101 tagtcccaat accggctcta tccatccggg agtaacttca atatcggagt tgagcagcac
     2161 ataatactcc gcgtcaatta atttcaacgc atcgttgtat cctttggcaa atccaccgtt
     2221 tgattgattt tgaattatcc tgatttccgg aaaatgttcc ttaagaaaag caacggaatc
     2281 gtcggaggaa gcattgtcgg caactatgat gctggcaata tttttactgt gctgtatgac
     2341 agcaggcaga aactgctcca ggaacttttt tccgttccag tttaatatta cgacggctgc
     2401 tttcaagaga gca
//
LOCUS       DAOSFP010000356         2399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_43213_length_2399_cov_3.591724, whole genome shotgun sequence.
ACCESSION   DAOSFP010000356 DAOSFP010000000
VERSION     DAOSFP010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43213_length_2399_cov_3.591724"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLI16_09340"
     CDS             complement(<1..809)
                     /locus_tag="PLI16_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOH84801.1"
                     /translation="MKSAEIRKTFLEFFKTKQHSIVPSAPMVVKNDPTLMFINAGMNQ
                     FKDYFLGNSIAPEKRVADTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWELLTGIYKINPDSLYVTIFEGDTHEGLAKDVEAYEQWKKIVPADRILMGS
                     KKDNFWEMGDTGPCGPCSEIHVDLRNDEEKLAVSGRELVNKGHPLVIEIWNLVFIEFN
                     RKADGSLVNLPAKHVDTGMGFERLCMTLQKVRSNYDTDVFQPLIQQIALLAG"
     gene            1003..1971
                     /locus_tag="PLI16_09345"
     CDS             1003..1971
                     /locus_tag="PLI16_09345"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOH84802.1"
                     /translation="MKKPKYHFNTKTLSFEEFKGSVKNTTLRFISILTSGMVISAAIM
                     FVLFSFVDSPKERALKRENEKMKLEYERLGERMDQISAVLANIQNRDDNIYRTIFEAE
                     PIDNAVRKAGYGGTDRYKSLQGYDNSEIMKDAAMRLDQISRQMYIQSKSFDEIVAIAK
                     NKEKMLSCIPAIQPVSKKRSTIISGYGYRIHPIYKTSRMHTGIDFMARTGTPVYATGD
                     GMVIDPKGNQSGYGIVCVINHGYGYQSLYAHMSKMIVKPGAKVKRGQVIGYVGSTGLS
                     VAPHCHYEIIKNGVKINPVNFFYNDLTPQEYEKVIEQASILNQALS"
     gene            1991..>2399
                     /locus_tag="PLI16_09350"
     CDS             1991..>2399
                     /locus_tag="PLI16_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84803.1"
                     /translation="MNRKFLKINGGNLHKERFSELKIYLFLLVIIIVGYYHVALFIHP
                     LKWDMIDQAYPYRYYIGECLRHGFFPYWLPYQQLGIPVCADPQSGVWYPITWIFGFFA
                     GYILYSLSAEYMLHVFLAAAGFYMLGKNLGLSKA"
BASE COUNT          677 a          520 c          487 g          715 t
ORIGIN      
        1 ccggccagca gggcaatttg ctgtatcaat ggctgaaaca catcggtatc gtaattagac
       61 cttacctttt gcaaggtcat acacaggcgc tcaaaaccca ttcctgtatc cacatgtttg
      121 gccggcagat ttaccagcga cccatcggct ttgcggttaa attcaataaa caccagattc
      181 catatttcaa taaccagcgg gtgtcctttg tttactaatt cgcgaccgct aactgccagt
      241 ttttcctcgt cgttgcgcaa gtccacatga atttcggaac aggggccgca aggaccggta
      301 tcacccattt cccagaagtt atcttttttg gaacccatta atatcctgtc agcaggtaca
      361 atttttttcc attgttcata agcttccacg tcttttgcta atccctcatg ggtgtctcct
      421 tcaaaaatcg taacgtacaa agaatcagga tttatcttat atatcccggt cagaagttcc
      481 caggcccatt cgatggcttc ctttttaaaa taatcaccaa aagaccagtt gcccagcatt
      541 tcaaacatgg tatgatgata ggtatcatgg ccaacctctt ccagatcatt gtgtttgccc
      601 gatactctca ggcatttttg tgtgtcggcc accctttttt cgggagcaat ggaatttccc
      661 aaaaaataat ctttaaactg gttcatcccc gcattgataa acatcagggt aggatcattc
      721 ttgaccacca tgggagcgga aggaacaatg ctatgttgtt ttgtcttaaa aaattcaaga
      781 aaagtttttc tgatttcagc tgatttcatg agttgtgtct gttacagtga atatttgtag
      841 ataagtattt gttttttaat cagttaatcc aacaaaatgc cggtttaact ttttaataac
      901 tcaaaaacga ctgcaaattt aatttttttt gttagctttg catttcgttt ataacatttt
      961 agctgaattt acgtttaaaa gtttttgaac caattgctgc tgatgaagaa accaaagtat
     1021 cattttaaca ccaagacact ttcgtttgag gaatttaaag gctctgtaaa aaacaccacg
     1081 ctgcggttta tttctatact cacatcgggc atggtaattt ctgcggccat catgtttgta
     1141 cttttcagtt ttgtggattc accaaaagaa agagctttga agcgtgaaaa tgaaaagatg
     1201 aaacttgaat atgagcggct tggtgaacgc atggaccaga tttcggcggt actggccaac
     1261 atacaaaacc gtgacgacaa tatttaccgg acaatttttg aagcagaacc cattgataat
     1321 gccgtacgca aagccggata cggcggaaca gaccgttata agtcgctgca gggttacgac
     1381 aactccgaaa ttatgaaaga cgccgccatg cggttggatc agatttcgcg gcaaatgtac
     1441 attcagtcga aatcctttga tgaaattgtg gccatcgcca aaaacaagga gaagatgctt
     1501 tcctgcatcc ccgccataca gccggtatca aagaaaagat ctactatcat ttcgggttat
     1561 gggtacagga ttcaccccat ttacaaaacc tcgcgcatgc acacgggtat agattttatg
     1621 gcccggacag gaacccctgt ttatgctacc ggcgacggaa tggtgattga ccccaaagga
     1681 aaccaaagcg gctacggcat tgtatgtgtg attaatcacg gttatggata ccagtcctta
     1741 tatgcgcaca tgagtaaaat gattgtgaag ccgggtgcaa aagtaaaacg gggacaggtc
     1801 atcggatatg ttggcagcac cggcctgtcg gtagctccgc attgccatta tgagattatc
     1861 aaaaacggcg taaaaataaa tcctgtaaac tttttctata acgacctgac accccaggaa
     1921 tatgaaaaag taatcgaaca ggcctccata ctgaaccagg cgctgtcgta acaaccttat
     1981 tccccctttt atgaaccgta agtttttgaa aataaacggg ggaaaccttc ataaggaacg
     2041 attttccgaa ttaaaaattt acttatttct tcttgtaata attattgtcg ggtattatca
     2101 tgtagccttg tttatacacc ccctgaaatg ggatatgatc gatcaggctt atccttacag
     2161 gtattatatt ggcgaatgcc tgcgtcacgg tttttttccc tactggttgc cctaccagca
     2221 actgggcatc cccgtttgtg ccgatcctca gagcggtgta tggtatccca taacatggat
     2281 tttcggtttt tttgccggat atattcttta ttcactttcg gcggaatata tgctgcatgt
     2341 ttttctggct gctgccggat tttatatgct tggcaaaaat ctggggttat caaaagcaa
//
LOCUS       DAOSFP010000357         2394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_43354_length_2394_cov_3.638735, whole genome shotgun sequence.
ACCESSION   DAOSFP010000357 DAOSFP010000000
VERSION     DAOSFP010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43354_length_2394_cov_3.638735"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="dnaJ"
                     /locus_tag="PLI16_09355"
     CDS             complement(<1..1016)
                     /gene="dnaJ"
                     /locus_tag="PLI16_09355"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOH84804.1"
                     /translation="MTKRDYYEILGVDRNCSEAELKKAYRQMAIKYHPDKNPGNKEAE
                     EKFKEAAHAYDVLSNPEKRSRYDRFGPDGVNGGNGFGGGSMSMDDIFSHFGDIFGGAF
                     SSFGGFGGGSSQRRKVNYGSNLRVRIKLTLEEIATGVEKKINVNKYVTCTDCKGTGAK
                     GGSSYKTCSTCNGRGHVVSVTNTFLGQMQTTSTCPTCGGEGQSITEKCTSCFGNGIIK
                     GDEVISIKIPAGVAEGMQLSVSGKGNAAARGGYPGDLIVLIEEIPHPDLHRDGNNLLY
                     TQYISFPEAVLGANVEIPTLEGKARVKIEPGTQSGKILKLKGKGLPSLNYHGRGDLLV
                     GINV"
     gene            complement(1027..1605)
                     /locus_tag="PLI16_09360"
     CDS             complement(1027..1605)
                     /locus_tag="PLI16_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOH84805.1"
                     /translation="MKKKGSTHMKDGQTDQQKVDENIQSDVTQETEQEQENHEQEKDG
                     IISEQKSRIEELNNKYLYLFAEFDNFRKRTSKEKLDLIKTASSGIVTAMLPVLDDFER
                     AIRAFPDDENMKSLKDGVVLIYEKYRAVLVKAGLEEIKAMGEEFNTDIHEAVANVPAQ
                     TEEQKGKIIDVTEKGYYLNGVVIRFAKVVVAN"
     gene            complement(1677..1967)
                     /locus_tag="PLI16_09365"
     CDS             complement(1677..1967)
                     /locus_tag="PLI16_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84806.1"
                     /translation="MAAPKKKKKRKIKYKKFEFKLSEKQKKIIDKFCRAKKVSPNKMI
                     KTAIRDYISKFADSLPEEDYISENQLQLFDVEEEQELFNSVPQENEFKETLF"
     gene            2094..>2394
                     /locus_tag="PLI16_09370"
     CDS             2094..>2394
                     /locus_tag="PLI16_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOH84807.1"
                     /translation="MINDKLFNPASIVVVGASNDVTKPGGKVLKNLLDGNYNGKIYVT
                     NLKETEVQGIRSFQNINDLPQTDLAIIAIAAKYCPETVEILAKQKQTRAFIILSAG"
BASE COUNT          587 a          569 c          447 g          791 t
ORIGIN      
        1 cacacattga tgccgacgag caggtcaccg cgtccgtgat aattcagcga aggcagacct
       61 ttccctttga gtttcaaaat ttttcccgac tgtgttcccg gttcaatttt cacccttgct
      121 ttgccctcca gtgtggggat ttcaacatta gcacccagca cggcctcagg gaaactaatg
      181 tattgagtgt acaagaggtt gttgccgtcg cggtgcaaat cgggatgtgg tatttcttca
      241 attagcacga taaggtcgcc cggatatccg ccacgagcgg cggcgttgcc tttgccgctt
      301 accgacagtt gcatgccttc ggccacgccg gcaggaattt ttatgctaat cacttcatcc
      361 cctttaatga taccgttgcc gaaacacgaa gtgcattttt cagtaattga ctgcccttcg
      421 ccaccgcacg tagggcaggt tgaggtagtt tgcatttgtc cgagaaaggt gttagtaaca
      481 ctcaccacat ggcctctgcc gttgcaggta gaacatgtct tataagaact tcctcctttg
      541 gcacctgtac ctttgcagtc ggtgcaggtt acatatttgt tgacgttaat ctttttctcc
      601 acaccggtgg caatttcttc caacgtcagt tttatcctta ccctgaggtt gcttccgtag
      661 tttaccttac gacgctgcga cgagcctccg ccaaaaccgc caaaactgct gaacgcaccg
      721 ccaaagatat cgccaaagtg gctgaagatg tcatccatgc tcatgctgcc accaccgaag
      781 ccatttccac cgttcacgcc atctgggcca aaacggtcgt aacggctgcg tttttcaggg
      841 ttactcaata catcataggc atgggctgct tctttaaatt tctcttctgc ttctttgttg
      901 ccggggtttt tgtcaggatg atacttgatg gccatctgac gataagcttt tttcagttct
      961 gcttcgctgc agttgcggtc aacacccaga atttcgtaat aatcgcgttt tgtcatacat
     1021 aaaagattag ttggcaacca ccactttagc aaaacgtatt accacgccat tcaggtagta
     1081 tcccttttcg gttacatcta tgatttttcc tttttgttcc tctgtctgtg cagggacatt
     1141 agcaacggct tcatgtatat cggtgttaaa ctcctcgccc atcgctttta tttcttcaag
     1201 accagctttt accaacacgg cacggtattt ttcataaatc aacaccacgc catctttcag
     1261 gcttttcatg ttttcatcat cgggaaaagc ccggatagcc ctttcgaaat catccaacac
     1321 cggcaacatg gctgtaacaa tgccagatga tgcagttttt atcaaatcca gtttttcttt
     1381 tgaagtacgt ttacgaaaat tatcaaactc ggcaaaaagg tacagatatt tgttattcag
     1441 ttcttcaatt ctgctttttt gctccgagat aataccatct ttttcctgtt cgtggttttc
     1501 ctgttcttgt tcagtttcct gcgttacatc agactgtatg ttttcatcaa ctttctgctg
     1561 gtctgtctgt ccatccttca tgtgtgtact ccctttcttt ttcataaagc ttttattctt
     1621 tttcaatgcc ataccttatc aatatttttg ccaataaagc acaatgcgac aatttgtcag
     1681 aacagggttt ctttaaattc attttcttgt ggtactgaat taaaaagttc ctgctcttct
     1741 tccacatcaa acaattgcaa ctggttttcg cttatataat cttcttccgg cagtgaatct
     1801 gcaaatttgc tgatataatc cctgatggca gtttttatca ttttgttggg acttaccttt
     1861 ttagcacggc agaatttatc aatgattttt ttttgctttt cagaaagttt gaactcaaat
     1921 tttttgtatt tgatctttct ttttttcttt ttttttggtg ctgccatggt aattttttta
     1981 taatccatta aaaagcggac aaaagtaata aaaattcaat tttttatcat acaattactt
     2041 tgatttggcc agcttttttt atcttagcac acttttattc aaaacattca ataatgataa
     2101 acgataaatt attcaatcct gccagcattg ttgttgttgg cgcatccaac gatgtaacca
     2161 agcccggtgg taaggtgctg aaaaacctcc tcgacgggaa ctataatgga aaaatatacg
     2221 ttaccaacct gaaagaaacc gaggtgcagg gcatacggag ttttcaaaat atcaatgatc
     2281 tgcctcaaac cgacctggcc atcattgcca tcgctgccaa atattgccct gaaacggttg
     2341 agattctggc aaaacaaaaa caaaccaggg catttatcat cctctcggcc ggct
//
LOCUS       DAOSFP010000358         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_43563_length_2386_cov_4.410553, whole genome shotgun sequence.
ACCESSION   DAOSFP010000358 DAOSFP010000000
VERSION     DAOSFP010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43563_length_2386_cov_4.410553"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PLI16_09375"
     CDS             <1..1546
                     /locus_tag="PLI16_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOH84808.1"
                     /translation="PLLLGLAGFFIQLNKDFKGFLVVLLLFIFTGLAINVYLNPVPYQ
                     PRERDYAYAASFYAFAMWIGLGVVSINQALQKLKKFIKPVILSIAAVILCMVFVPVLM
                     AREGWDDHDRSNRYTARDIAADYLNSCAPNAILFTLGDNDTFPLWYAQEVEGIRTDVR
                     VVNLNLLNMDWYIDQMKHKVYDSDPLPITLDHKKYIASNRDYIFVYEDTNLVKPGAYA
                     KLNALVDFATSDDDALKINTTRGKLNYFPTRKFSLPVDTALVLKNGTVPPEYADSVLT
                     DIRWNYDGHVVMKNGLAVFNLLSELNWKRPVYFASTTGDDAYLNLLDYLQLEGLAYRL
                     VPYRCHSIDQETGRVNTTIMYENMMNRFAYGNMNCDGIYLDETNMRLTNTFRKLFSRL
                     ATALQYEGKPDKALQVCDKCIEVMPDNTVPYNYMLLPIIKVYYQCGQKEKANQIVTRL
                     GDYCEQELRYFNQFKGTQAVYISMEKNDALDLLKKLTDISKTYNQSALEKQLNTVFEK
                     YNTAKN"
     gene            1609..2268
                     /locus_tag="PLI16_09380"
     CDS             1609..2268
                     /locus_tag="PLI16_09380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOH84809.1"
                     /translation="MYLTKTPFLLQKLYFTNDLWCVETDHNEIYLTFDDGPEPEVTPW
                     VLDLLDQYNAKATFFCVGNNVGKNPELFSEILRRGHSIGNHTYSHVNGWRSNLYNYLL
                     NVKKCNRHFKTNLFRPPYGKISARQRTLLKKHFRLIYWTVISGDFDKTISPEICLEKT
                     ISNTEKGAIIVFHDSQKAKKNLTFTLPAFLKHFHNKGFSFKAIPCEIDKPKLAPVRNT
                     L"
BASE COUNT          723 a          597 c          471 g          595 t
ORIGIN      
        1 gccgctgctg ttaggcctcg ccggattttt cattcagcta aacaaagatt tcaaaggatt
       61 tctggtggta ttgttattat ttatttttac cggtttagcc atcaacgtgt acctcaatcc
      121 ggtgccctat cagccgcgcg aacgcgatta cgcctatgcg gcatcattct atgcctttgc
      181 catgtggata gggctgggag ttgtcagcat caaccaggct ttgcaaaaac ttaaaaaatt
      241 tatcaaacca gtcatcttat cgattgcagc cgttatttta tgtatggtat ttgtgcccgt
      301 tctgatggcc cgtgaaggct gggacgacca cgatcgttcg aaccgttaca ctgcgcggga
      361 cattgccgcg gactacctca actcctgcgc tcccaacgcc atcctcttta cactgggcga
      421 taacgatact tttcccttgt ggtatgccca ggaggtggaa ggtatacgca ccgatgtgcg
      481 ggtggtaaat cttaacctgc tcaatatgga ctggtacatc gaccagatga agcataaggt
      541 gtacgactct gacccgctgc cgataaccct cgaccataaa aaatatatcg cctccaaccg
      601 cgattatatc tttgtatatg aagacaccaa cctggtgaaa cccggagctt acgccaaact
      661 caatgcactg gttgattttg cgaccagcga cgacgatgcc ttgaaaatca acaccaccag
      721 aggaaaactg aactattttc ccacccgaaa attctcacta cccgtagata cggcactagt
      781 actcaaaaac ggcaccgtgc cacctgagta tgccgattcg gtattaacgg atatccgctg
      841 gaactatgat ggacatgtgg tgatgaaaaa cggactcgcc gtctttaacc tcttatcgga
      901 attaaactgg aaacgtcccg tatattttgc atccaccacc ggtgatgatg cctatctgaa
      961 tctactggac taccttcaac tggaaggact ggcttaccgt ctggtgccct accgttgtca
     1021 ctccattgac caggaaacag gaagagtgaa taccaccata atgtatgaga atatgatgaa
     1081 caggtttgcc tacggcaaca tgaactgtga tggtatttac cttgatgaaa ccaatatgcg
     1141 cctgaccaac acctttagga aactcttctc aaggctggcc acagccctgc aatacgaggg
     1201 gaaaccagac aaggctctgc aggtttgcga caaatgtatc gaagtaatgc ccgacaatac
     1261 cgttccctac aactatatgc tcctgccgat aatcaaggtg tattatcagt gcgggcaaaa
     1321 agaaaaagcc aaccaaattg taacccgcct cggtgactat tgcgaacagg aactcaggta
     1381 ttttaatcag ttcaaaggca ctcaggcggt gtacatctct atggaaaaaa atgacgcgct
     1441 cgacctgctg aaaaaattaa cagatatctc aaaaacatat aatcaatcag cactcgaaaa
     1501 acaattgaac accgtttttg aaaaatataa cacggcaaaa aactgaccgg aacttatcat
     1561 cactttttta aaaaaatatt attttctttg taaattattt cgttgagaat gtaccttacc
     1621 aaaacgccat ttttattaca aaaactatat tttaccaatg acctctggtg tgtagaaaca
     1681 gaccataatg aaatctacct gacttttgac gacggccccg aacctgaagt aaccccatgg
     1741 gtgctcgacc tgctggatca atacaatgcc aaggccacat tcttttgtgt gggcaacaat
     1801 gttggaaaaa accccgaact gttttccgaa attctccggc gtggccacag catcggaaat
     1861 cacacctaca gccatgttaa cggctggaga tccaacctgt ataattacct gcttaatgta
     1921 aaaaaatgca acaggcactt taaaaccaat ttgttcaggc ctccttacgg caaaatctcg
     1981 gcacggcaac gcaccctgct gaaaaaacat ttccggctga tttattggac cgtcatcagc
     2041 ggtgattttg acaagaccat ttcgccggaa atctgcctgg aaaagaccat ttcaaatacc
     2101 gaaaaaggag ctatcattgt ttttcacgac agccaaaaag ccaagaaaaa tctcaccttt
     2161 acccttccgg catttttaaa acactttcac aacaaaggct tttcttttaa agccattcca
     2221 tgcgaaatag acaaacccaa actggcaccc gtccgcaata cactataaac ccaatgagca
     2281 taaaaacagc agaacgtaat tcagggatac ttcccgaaga caatgtgata caccaccggc
     2341 acctgattgc ctatcaggaa gccgccaaat tatgccatgg caaagt
//
LOCUS       DAOSFP010000359         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_43575_length_2386_cov_2.747319, whole genome shotgun sequence.
ACCESSION   DAOSFP010000359 DAOSFP010000000
VERSION     DAOSFP010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43575_length_2386_cov_2.747319"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLI16_09385"
     CDS             complement(<1..322)
                     /locus_tag="PLI16_09385"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HOH84810.1"
                     /translation="MDGTSDQQPAMASKKFIEIEKVIGKKNPRLLKLLPGFVIRYIKR
                     IIHQDEINDFIERNGNVYGLEFIDRIVKEFIKELNTNGIENIPADRRILIASNHPLGG
                     LDGVA"
     gene            complement(398..1696)
                     /locus_tag="PLI16_09390"
     CDS             complement(398..1696)
                     /locus_tag="PLI16_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOH84811.1"
                     /translation="MNFSKIPAIECKPVAEIVRFQEKQLRAELKYLQDYSKYYRRFFK
                     KHHIDIRKIKTLKDMQHIPVTTKDDLQRYNDDFICVPRSKVVDFMTTSGTLGYPITLA
                     ATENDLQRIAYDEALNFACAGAGKQDVFQLMTTLDRRFMAGLAYFMGLRRLGAGVIRV
                     GSGMPELHWDTIRRFNPTYIVTVPSFILKLVDFARANGIDYLNSSVKNAVCIGEPLRN
                     PDFSLNTLGSRINERWPLKLFSTYASTEMGAGFTECPQGMGGHIHPELIIIEFLDENN
                     MPVADDEAGEITITTLGVEGMPLLRYKSGDVCFHHNEPCACGRTTMRLGPVIGRKQHM
                     IKYKGTTLYPASLYDILDGFDTIENYVVEVSTNEIDTDRICIYIGCRDHVSGFEKKIQ
                     DRFRAKLRVVPEIIFQSAEAVNRMAFPPMSRKPVKFIDKR"
     gene            complement(1754..2029)
                     /locus_tag="PLI16_09395"
     CDS             complement(1754..2029)
                     /locus_tag="PLI16_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOH84812.1"
                     /translation="MEVNDELKLKIKQMIVDTLKIKDVNPSDILNDQPLFSGENIITL
                     DSIDGIELIMAIQRQFGVRLDDQNLARNILNTVDSMAAFIADGIEKA"
BASE COUNT          687 a          549 c          486 g          664 t
ORIGIN      
        1 atgccacccc atcgagccct cccagcggat ggtttgacgc aatcagtatc cgtctgtcag
       61 caggaatgtt ttctattccg ttagtattta gttctttaat aaactctttg acaatccggt
      121 cgataaactc cagcccgtac acattgccgt tgcgctcaat aaaatcgtta atctcgtcct
      181 gatgaataat ccgctttata taacgaatga caaacccggg caaaagtttc agcaaccggg
      241 gatttttctt gccgataacc ttttcgatct cgatgaactt tttggaagcc atggcaggct
      301 gctggtcgct ggtaccatcc ataaatgcaa aatattttag aagtgtaaag ttaggtcttt
      361 ttttgtgaaa tttcaaaaat atgcaaagta agagcaatta ccttttgtcg ataaacttaa
      421 ccggcttacg gctcatgggc ggaaaggcca tacggtttac agcctccgca gactgaaaaa
      481 ttatttcagg aactacccgt aattttgccc tgaaacgatc ctgtattttt ttttcaaaac
      541 cagatacatg gtcacggcat ccgatataaa tacaaattct gtccgtatca atctcgttgg
      601 tggaaacctc caccacatag ttttcaatag tatcaaaacc gtcgagtatg tcatacaaag
      661 aagccggata taaggttgtt cccttgtatt tgatcatatg ctgttttcgg ccgataacgg
      721 ggcccagcct catggtggtt ctgccgcagg cacagggttc gttatgatga aaacacacgt
      781 cgcccgactt ataacgcagc aatggcatcc cttccacacc cagcgtggta atagtgattt
      841 ctccggcttc atcatcagcc accggcatat tgttttcgtc aagaaactca atgatgataa
      901 gttcaggatg gatatgaccg cccatcccct gaggacattc ggtaaacccg gcacccattt
      961 ctgtggaggc gtaagtagaa aataatttca gcggccatct ttcgttaatg cgtgaaccca
     1021 gggtattaag agaaaaatcg ggattacgca atggttcgcc aatacatacg gcatttttta
     1081 ccgaagaatt caggtaatca atcccgttgg ctcgggcaaa atcaaccagc ttcaaaataa
     1141 aagacggtac ggtaactatg taggttgggt taaacctcct gatggtatcc caatgcagct
     1201 cgggcattcc cgaacccacc cggatgactc ctgcccccag ccggcgcaac cccataaaat
     1261 aggccaaccc tgccataaac cggcggtcca gtgtcgtcat caactgaaac acatcctgtt
     1321 tgccggcccc ggcacaggca aaattcaagg cctcgtcgta ggcaatgcgt tgcaagtcgt
     1381 tttcagtggc tgccagcgtg ataggataac ctaaagtgcc ggaagtagtc ataaaatcca
     1441 ccaccttgct cctgggcaca cagataaagt catcgttata ccgctgcaga tcatccttgg
     1501 tggtaaccgg aatgtgttgc atatccttca gagtttttat cttgcggata tcaatgtggt
     1561 gttttttaaa aaaccgccgg taatacttcg aatagtcctg taaatatttc aactctgccc
     1621 gcagctgttt ttcctgaaac ctgacgattt cggccacagg cttacattca atagcgggaa
     1681 ttttagaaaa gttcatgatt aaaaaatgaa agtaacaaca aaaacattaa taggtatcac
     1741 cattaaaaaa gccttacgct ttttctatac catcggcgat aaatgccgcc atactatcca
     1801 cggtattcaa aatattgcgg gcaaggttct gatcgtcgag acgcacccca aattgccgtt
     1861 ggatagccat tataagctct ataccatcaa tggaatccag ggtaatgata ttttcacctg
     1921 aaaaaagtgg ctggtcatta agaatgtcgg aggggttgac atccttgatc ttcagtgtgt
     1981 ccacaatcat ctgttttatc ttcaacttca gttcatcatt tacttccata agtttgtttt
     2041 tattcacatc aaatatctga caaaattatt aattttttta taaaagtact caatcatttt
     2101 ttgaggtgct gaaagagact attttatttt aaataactca acttccgcaa ttcagttaaa
     2161 attagcctga cctattcata aatgaatgaa gttaaatttg tgaatgtgcg tggacgttgt
     2221 ggacagcaga tcagagtgtg ggtcgggagg gctaataagc aacatttgta gtgggggcat
     2281 gacttgcttc agcacatgac cacgcgaagt catactccca cttgcaaatg gaaaaaagtt
     2341 accaaacccg cccgtcatgc taacgccggt tataacagcg ggaaac
//
LOCUS       DAOSFP010000360         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_43859_length_2376_cov_2.846187, whole genome shotgun sequence.
ACCESSION   DAOSFP010000360 DAOSFP010000000
VERSION     DAOSFP010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43859_length_2376_cov_2.846187"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLI16_09400"
     CDS             complement(<1..730)
                     /locus_tag="PLI16_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOH84813.1"
                     /translation="MFAIIDIETCGGKYEYRKGAITEICILIHDGLKVVDKFTTLINP
                     QCHINHFYRNISGITDEMVADAPKFYEVAGKILEMTENRIFVAHNVSFDYNFIKSEFA
                     SLGYKFNREKLCTVRMARKLLPGRISYSLGHLCASLGIEIEGRHRAEGDAVATAQLFD
                     LLLQLKSQDPHYRNMGVEGIMTRRIDKIKEYILKKLPEECGVYYFLDKNQHIIYIGKS
                     VNMYQRAISHFNTQEKKGKNMLNDL"
     gene            complement(1010..1789)
                     /locus_tag="PLI16_09405"
     CDS             complement(1010..1789)
                     /locus_tag="PLI16_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HOH84814.1"
                     /translation="MSKIAYNIRYLRELKKLSQEGLAEDLKITRARLGAYEEARNEPP
                     IEMLIRLSEYFKISVDALIKADLRKTDVNALMKIGDNRLLFPVILDKDNNDRIEVITA
                     KASAGYLNGYADPEYIEKMPFMELPFSVTGKHRAFPVKGDSMPPLATGDFVIGKYVES
                     LKEVLDGKTYVLLTKSEGIVYKRIYKKPGFVVELHSDNKSYQPYVINAYDILEIWEFV
                     CCLKTSDRKKDEINMEHIINLLLSMKVELEQLKKNDGKIWK"
     gene            1864..>2376
                     /locus_tag="PLI16_09410"
     CDS             1864..>2376
                     /locus_tag="PLI16_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOH84815.1"
                     /translation="MNRCIVHLDLDTFFVSVERLMDSRLNGKPVIIAGASDRGVVASC
                     SYEARAFGVHSAMPAKLAKQLCPQAVFVMGDMENYSKYSDQVTEIIKDQAPLVEKASI
                     DEHYIDITGMDRYIKNSMLWTHELRQTIIKESGLPISFGLSVNKTVSKVATGEAKPSG
                     EKQVDAGIEKL"
BASE COUNT          628 a          514 c          503 g          731 t
ORIGIN      
        1 acaggtcgtt gagcatattt tttccttttt tctcctgtgt gttgaaatgg ctgatagccc
       61 gttggtacat gttaacactt ttgccaatat aaatgatgtg ttgatttttg tcgagaaaat
      121 aataaacacc gcattcttct ggtagtttct taagtatgta ctcttttatt ttatcgatcc
      181 tgcgtgtcat gattccttca acgcccatgt tacgataatg aggatcctgc gacttgagct
      241 gcagtaacaa gtcgaaaagt tgtgcggtgg ccactgcatc gccttcagcc ctgtggcggc
      301 cttcaatttc gatgcccagc gaagcacaca aatgccccag gctgtacgaa atccttcccg
      361 gcagcagttt gcgggccata cgcacagtac aaagcttttc ccgattaaat ttatatccca
      421 gagaggcaaa ttcgctttta atgaaattat aatcgaagct cacattgtgg gcaacaaaaa
      481 tcctgttttc ggtcatttcg agtattttgc cggccacttc ataaaatttc ggcgcatcgg
      541 ccaccatctc atcggtgatg cccgaaatat tccggtaaaa atgatttatg tggcactgcg
      601 gattaataag agtagtaaat ttatcgacta ccttcagacc gtcgtggatc aggatacata
      661 tttcggtgat ggcgcctttg cggtattcgt acttgcctcc acaggtctct atgtcgatga
      721 ttgcaaacat tttttattgg atgcggattg atattctaaa atcaaaatta ttcattactt
      781 gggttcggga tagataaatt attgttgttt tttcaagatg ctaattatgt gaatcgtgtt
      841 taaatatttt ttttaccaca aaggcgctca aaggttgaca caaagtaaca caaagtatat
      901 ttttgagatt attttttttt taattttacc ttcgtgtccc tttgtgaatg tacttcgtgt
      961 ccaccgccgg actttgtggt aaaatttaat ttctaacctt ggattcgcat tacttccaaa
     1021 tttttccatc atttttcttc agttgctcaa gttccacttt catgctcaac aacaggttga
     1081 taatatgttc catatttatc tcgtcctttt tcctgtcgga ggttttgagg cagcaaacaa
     1141 actcccatat ttcaaggata tcgtaagcgt taataacata gggctgatac gacttgttat
     1201 cggagtgcag ttcaacgaca aaccccggct ttttataaat acgtttatag acaatacctt
     1261 cactttttgt tagtagcacg taggttttac cgtcaaggac ctctttcagg gattctacat
     1321 atttgccgat tacaaaatcg cccgtagcca gcgggggcat cgaatcgccc tttacaggaa
     1381 aagccctgtg tttgccggtt acgctaaaag gcagttccat gaaaggcatt ttttcaatgt
     1441 attcaggatc agcataacca ttcaggtatc ctgccgaagc cttggcggta atcacttcga
     1501 tgcggtcgtt gttgtcttta tcaagaatga cgggaaaaag aagcctgtta tcgcctatct
     1561 tcatcagggc attgacatct gttttccgca aatcggcttt gataagggca tcaacagaaa
     1621 ttttaaaata ctccgagagc ctgatgagca tttctatcgg cggctcgttg cgggcttctt
     1681 cgtaagcacc aagacgggcg cgggtaatct ttaaatcttc ggcaagacct tcctgcgaaa
     1741 gcttttttaa ctccctcaga tacctgatgt tataagctat tttactcatt actacatttt
     1801 atagcacaaa tatactactt tttgtagtaa ctttgcaaat aaaaaatttt tttttaaaaa
     1861 atcatgaacc gttgtattgt gcatctcgat ctggacacgt tctttgtttc cgtggagcgg
     1921 ctcatggaca gccgtcttaa cggcaaaccc gtgatcatag caggcgcttc ggaccgtggg
     1981 gtggtagctt cgtgcagcta cgaagcacgg gccttcgggg tacattcggc tatgccggcc
     2041 aaactggcca aacagctatg tccgcaggca gtttttgtga tgggcgatat ggagaactac
     2101 tcgaaatatt ctgaccaggt gacagaaatt attaaagatc aggcgcctct ggtagaaaaa
     2161 gcttctattg atgaacatta tattgacatt accggcatgg accgctacat caaaaacagc
     2221 atgctgtgga cccatgagct gcggcagacc atcatcaaag agtcgggact gcctatttcg
     2281 ttcgggctat cggtaaacaa aaccgtgtcg aaggtggcca ccggcgaagc caagcccagc
     2341 ggagaaaaac aagtggatgc cggcatcgaa aagctg
//
LOCUS       DAOSFP010000361         2365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_44133_length_2365_cov_3.355844, whole genome shotgun sequence.
ACCESSION   DAOSFP010000361 DAOSFP010000000
VERSION     DAOSFP010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44133_length_2365_cov_3.355844"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="PLI16_09415"
     CDS             <1..359
                     /locus_tag="PLI16_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH84816.1"
                     /translation="MIEAGAEGFLLKKTNKDELQRAINQVMAGENYFSEEFISNVNRN
                     LRPSLRMAGIELTDREQEVLELICKGMSNAEISKYLGVSARTVDGHRAHLLEKTGAKN
                     SPHLVMFAIKNGLIKA"
     gene            complement(454..1584)
                     /locus_tag="PLI16_09420"
     CDS             complement(454..1584)
                     /locus_tag="PLI16_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HOH84817.1"
                     /translation="MLSEVHKKNNDELYKTSIVQQTAFWSLVKRKTGRETIAINFKSK
                     KSHLFHDAAVDYDINSDLLVVILQVNKHDSIAYVPYGPELEPDNEFQGLFLEELSECL
                     RSFLPAACFMIRYDLCWEVFWAGDNAYFDHTGNVIDSPSLKSQELRLNFNTFNWNLRK
                     AQTNILPTNTLYISLEADTATILERMKPKTRYNIKLSKKKGVTVRIADISDLHIWYQL
                     YSDTAQRNGLYLNNINYFEAILTTRADKTKSPAEVYYLIAEYDKTPLAAMFLIITGQR
                     GSYLYGASSSINRNFMATYALQWEAIKISKEKGCIEYDMFGISPKNDISHPLYGLYRF
                     KTGFGGEMFHSLGCWDYFFDKEKYQQFIGTEMVNPGYHLTTK"
     gene            complement(1707..2003)
                     /locus_tag="PLI16_09425"
     CDS             complement(1707..2003)
                     /locus_tag="PLI16_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84818.1"
                     /translation="MKKIIINKLDYLRIKQSISNAKQSNRISAFESEKLVNELESAEI
                     IEPEKIPSNVLTMNSKVKGIFLNSRQQLTFQIVYPHQAHLKKIKFPFFRKWPQP"
BASE COUNT          703 a          465 c          479 g          718 t
ORIGIN      
        1 agatgattga agcaggggcg gagggttttc tgttgaaaaa aaccaacaaa gacgagctgc
       61 agcgggctat caaccaggtg atggccgggg aaaattattt ttcggaagaa tttatcagca
      121 acgtaaaccg taatctgagg ccttcgctga ggatggccgg tatcgagctt acggaccgcg
      181 aacaggaagt gctcgaacta atatgcaaag gcatgtcaaa tgccgaaatt tcaaaatacc
      241 ttggcgttag tgcacgcacc gtggatggac acagggcgca tctgctcgaa aaaactggtg
      301 cgaaaaactc tccgcacctg gtgatgtttg ccataaaaaa cgggttaatc aaggcctgag
      361 aactgtcgtt gaccgcccgg aatccgccca acagccttca ttgcaagaca tgccttttat
      421 aggcattcag gaatgtttgt caatcgaaca ggatcatttt gtggtgagat gatatcccgg
      481 atttaccatt tccgtgccta taaactgctg gtatttttct ttgtcgaaaa aataatccca
      541 acagccaaga ctatgaaaca tttccccgcc aaaacctgtt ttaaaccggt ataagccata
      601 aagagggtgg gagatgtcat tttttggcga aatgccaaac atatcatatt ctatgcagcc
      661 tttttccttt gagattttta tagcttccca ttgcagggca taagtagcca taaaattcct
      721 gtttatgctt gatgaggctc catacaaata agagccgcgc tgcccggtta ttatcaaaaa
      781 catggcagcc agtggcgtct tatcatattc ggctattaaa tagtaaacct cggcaggcga
      841 tttggttttg tcggcgcggg tggtcaaaat agcttcaaaa taattgatgt tattcagata
      901 caatccgttt ctttgtgcgg tatctgagta caattgatac cagatgtgta aatcgctgat
      961 atcagctatt ctcacggtaa cgcctttctt tttcgagagt ttgatattat atctggtttt
     1021 aggcttcatg cgttccagaa tggtggcggt atctgcttcc agactgatat acaaagtgtt
     1081 tgtcggtaaa atattcgttt gcgcttttcg gagattccag ttgaaggtat tgaaatttaa
     1141 cctcaattcc tgcgacttca aagaaggtga atcaattaca ttgccggtat ggtcaaagta
     1201 ggcattgtct ccggcccaaa aaacctccca gcacaaatca tacctgatca taaagcaggc
     1261 ggcaggcaaa aaagatcgta aacactccga tagttcttct aaaaaaagtc cctgaaactc
     1321 attgtcgggt tccagttccg ggccataggg gacataagca atactatcgt gtttattcac
     1381 ttgcaaaatg accaccagaa ggtctgaatt gatatcataa tccaccgcgg catcatgaaa
     1441 aagatggctc ttttttgatt taaaattgat ggcaatggtt tcacggcctg tttttctttt
     1501 gacaagtgac caaaaggcgg tttgttgcac tatagaggtt ttatatagct cgtcattgtt
     1561 ttttttatgt acttctgata acatgataat tctttataag gtgtaatcac cagctgcttc
     1621 gggttggtat ataatttcat ctatcctgat cgtcgttaag ccggcgggaa caatccattc
     1681 gattaaatcg ccaacacggt aacctattag ggctgtggcc acttgcgaaa aaatggaaat
     1741 tttatttttt tcaaatgggc ctgatgtggg taaactatct gaaatgtcag ctgttgacgg
     1801 ctgtttaaaa aaataccttt tactttggag ttcatagtta acacgttcga cggaattttt
     1861 tccggttcaa tgatttccgc cgactccaac tcgttaacta atttctctga ttcaaatgcg
     1921 gaaatacggt ttgactgttt tgcattgctt atcgactgct ttatccgcaa ataatcaagc
     1981 ttgttaataa taattttttt catgtctttt attttttatt acaatcaaaa acatgcaata
     2041 ccgtcgggta gagctaatta aaaaggtctg ttgccatttg ccgaacctcc aaaatttcaa
     2101 aaatacaaat ttattcaaga ggggcaaaat ccgctcaggt tcaattcctg tttatgatag
     2161 gtaaaaaaca ataccctatc aagaagtaaa ccgttgatta tttgcagtta aacacttttc
     2221 acgaaacaca tgcctggctt aataagaaaa tatcttaatc attcaaaaac aagtgaaaag
     2281 accatgtggt aatttcccgg atattatacg tatatttacc cgttgaaaaa taaattattt
     2341 ttttaaagaa aatatcaatt ttgca
//
LOCUS       DAOSFP010000362         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_44451_length_2354_cov_3.030883, whole genome shotgun sequence.
ACCESSION   DAOSFP010000362 DAOSFP010000000
VERSION     DAOSFP010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44451_length_2354_cov_3.030883"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLI16_09430"
     CDS             <1..397
                     /locus_tag="PLI16_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOH84819.1"
                     /translation="VIAALVIIVDQVLKAYVYDVSKKLSVFVGLIITNCILMGRLEAF
                     AMANKPWPSFLDGIGNGLGYGLILVIIAFIRESLGSGTLFGFHIIPQTFYDHGYMNNG
                     MMILPPMALIIIGVIIWVQRSFNKSLIEK"
     gene            409..1026
                     /gene="nqrE"
                     /locus_tag="PLI16_09435"
     CDS             409..1026
                     /gene="nqrE"
                     /locus_tag="PLI16_09435"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOH84820.1"
                     /translation="MNDLVNIFVRSIFIDNMIFAYFLGMCSYLAVSKTVKTSFGLGVA
                     VVFVLVITAPVNWLIDTYLLKPGALSWLGSGYSDVDLSYLSFIIFIAVIAAIVQLVEM
                     VVEKFSPSLYNALGIFLPLIAVNCAILGLSLFVQEREYTAISEVTVYALGSGIGWLLA
                     IVSIAAIREKIKYSNVPVPLRGLGITFILTGLMAIAFMSFMGIKI"
     gene            1057..2316
                     /gene="nqrF"
                     /locus_tag="PLI16_09440"
     CDS             1057..2316
                     /gene="nqrF"
                     /locus_tag="PLI16_09440"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOH84821.1"
                     /translation="MILLDTGLGLILVLGITIFLVISLLLVLLLLFAKAKLSPSSPVL
                     ININSDKEVEVNSGSSLLSTLSENKIFLPSACGGGGTCGMCRCQVISGGGSILPTEVG
                     FFTRKQIQDNWRLGCQVKVRENLEIQIPKEIFGIKKWECEVVSNKNVATFIKEFVVKL
                     PEGETLDFQSGGYVQIDVPKCEVDYKDILVEEKFRSDWEKFRMFDLKMKNPEPVYRAY
                     SMANHPAEGNIVMLNIRIATPPWDKKINGFMKVNPGICSSYIFSRKPGDKVMVSGPYG
                     EFHIKHTDKEMVYIGGGAGMAPLRSHIFHLFHTEHTKRKVSYWYGGRSMREIFYEDHF
                     RAIEKENSNFTFNIALSEPLPEDNWTGYTGFIHQVVYDNYLSKHPEPEDIEYYLCGPP
                     MMNDAVFKMLDKLGVPKENIAYDDFGG"
BASE COUNT          644 a          482 c          548 g          680 t
ORIGIN      
        1 agttattgcc gccctggtaa ttattgttga tcaggtgctg aaagcctatg tgtacgatgt
       61 aagtaaaaaa ctttcggtat ttgtgggtct tatcatcacc aattgcattt tgatgggccg
      121 cctggaagcg tttgccatgg ccaataagcc gtggccctcc tttcttgacg gcatcgggaa
      181 tggccttggc tatggtttaa ttcttgtgat tattgcattt atcagggaat cgcttggctc
      241 cggcaccctg tttggctttc acatcattcc gcaaaccttt tatgatcatg ggtacatgaa
      301 caacggcatg atgattctgc cgcccatggc tctgatcatc attggagtca ttatttgggt
      361 gcaacgcagt tttaataaaa gtttaattga aaaataaaat tcaacgatat gaatgacctg
      421 gtaaatattt ttgtccgctc gatattcatt gacaatatga tatttgcata tttcttagga
      481 atgtgctcat atctggcggt atcaaagact gtgaaaacct cgttcgggtt gggtgtcgcg
      541 gtggtatttg tgctggttat tacggcccca gtcaattggc ttattgacac ctacctgtta
      601 aaacccggcg ccctgagttg gcttggcagc ggatattcgg atgtagatct cagttacctg
      661 agttttataa tttttattgc cgtaatagca gcaatagtac aactggttga gatggtagtt
      721 gaaaaattca gtccttcgct gtacaatgcg ctgggtatct ttctcccgtt gattgctgtt
      781 aactgcgcca ttctgggact ttccctcttt gttcaggaaa gggaatacac ggccatcagc
      841 gaagtaaccg tttatgccct gggctcgggc atcggctggc tgctggccat agtgtctatc
      901 gcagcaatac gtgaaaagat taagtattcc aatgtcccgg tacccctcag gggactaggc
      961 attaccttta ttcttaccgg gcttatggcc attgccttta tgagtttcat gggaataaaa
     1021 atctgacaaa caaaacaatt aaaaaaacct gaaattatga tattgctgga tacaggatta
     1081 ggattaatat tggtactggg cattacaatc tttttagtaa taagtttgtt gctggtactt
     1141 ttgcttttat ttgctaaagc aaaactgtcg ccatcaagcc ctgtgctgat aaacataaac
     1201 agcgacaaag aagttgaggt gaactcaggt tcttcgcttt tgtccacctt gtcggaaaac
     1261 aagatttttc tgccttcggc ctgcggcggc ggaggaacct gcggtatgtg ccgttgccag
     1321 gtaatcagcg gcggtggcag tattttaccc actgaggtag gttttttcac ccggaaacag
     1381 atacaggaca actggcgcct gggctgtcag gtaaaagtaa gagaaaacct ggaaatccaa
     1441 atcccgaaag aaatctttgg cataaagaaa tgggaatgtg aagtggtgtc aaataaaaac
     1501 gtagcaactt ttatcaaaga atttgtagta aaacttcctg aaggagaaac ccttgatttt
     1561 caatcgggag gttatgtaca gatagatgtg cccaaatgtg aagtcgatta taaagacatc
     1621 ctagtggagg aaaaatttcg cagcgattgg gaaaaattca ggatgtttga tctgaaaatg
     1681 aaaaaccccg agccggtgta ccgggcatac tccatggcca accacccggc tgaaggcaat
     1741 atcgttatgt tgaatatacg tattgccact ccgccctggg ataaaaaaat caatggtttt
     1801 atgaaggtaa acccgggtat ttgttcgtct tatatctttt cgcgtaaacc gggcgacaaa
     1861 gtgatggtat ctggcccata cggagagttt catatcaaac ataccgacaa agaaatggtg
     1921 tatatcggcg gcggcgccgg catggcacct ttacgttcgc acattttcca cctgttccat
     1981 acggagcaca ccaaacgcaa ggtttcatac tggtatggcg gccgctcgat gcgtgagatt
     2041 ttttatgaag accatttcag ggctattgaa aaagaaaatt cgaattttac cttcaatatc
     2101 gccctttcgg agcctttgcc cgaagacaac tggacaggct atacaggatt tattcaccag
     2161 gtggtttatg acaactactt aagcaagcat cccgaaccgg aagatattga atactatctc
     2221 tgcggcccac cgatgatgaa tgacgcggta tttaaaatgt tagacaaatt aggagtgcca
     2281 aaagaaaata tcgcttacga cgattttggc ggttaggcag tgctgtcaat ttgccagttt
     2341 cagccccact atac
//
LOCUS       DAOSFP010000363         2332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_45110_length_2332_cov_4.177866, whole genome shotgun sequence.
ACCESSION   DAOSFP010000363 DAOSFP010000000
VERSION     DAOSFP010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45110_length_2332_cov_4.177866"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLI16_09445"
     CDS             complement(<1..443)
                     /locus_tag="PLI16_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84822.1"
                     /translation="MKKYLTFILTVILSGSITAQKYYTGQAPEAAQILKTRGEIVVSF
                     VVNEKSQINNDLTKILSIDNVKQLPGGQGYQVRAYANQQEYEEFLSRNIPYEIIPKII
                     PKALTMATTVAEMTNWDRYPTYSVYEQMMANFAITYPGLCDIDTIL"
     gene            complement(613..>2332)
                     /locus_tag="PLI16_09450"
     CDS             complement(613..>2332)
                     /locus_tag="PLI16_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOH84823.1"
                     /translation="TPGLLSITGSDAGSTYYYYFYEWLVQKDACVSSRVPAVASILTV
                     PTAGFSSSVSTYTATFTNTSTNSTGYAWNFGDGGTSAQQNPVHTYATSGTYNVRLITT
                     NGSCSDTIYHNVTITAGSNYSITGKTRYLSKATAGIAPGMPTYNPAIYNIGKVLVILK
                     SSPAGIELARDTTNSQGVFQFNNVANGNYILSYDKYTADTMQMCNDVNAIDLAIMKYL
                     VGHDTTSDPSRKFSAKHLKAANVDNNATINSIDIARMSAKIGQPYSASANFPKGNWVA
                     FDTAVTVAGANLNLTLKTIGYGDYNASSTSYKDSVNTWSTAKNLEKNTILISSEETSV
                     INDPSYVEISLTSSNLISDFAALGLELNYSAEDYKLVSAVMPNNKTKGTPTKINPSLE
                     EIIAENNDLLVTDYNGVIRVVYATTNIFDIKAHDTVITLGFISRNGINPGMIDIITSG
                     TGVIGDQYGNEVTDAYLIMPKIFLQNDANHQETLYFEGHPNPVNDNATILFNLPENGT
                     VTLKVFNAIGEQVAILMNETCQRGKNQTEFSAKNLRDGFYTLKLEFAGSEKSGCHILK
                     MIVQHR"
BASE COUNT          592 a          454 c          539 g          747 t
ORIGIN      
        1 agaatggtgt ctatgtcgca caggcccgga tacgtaatgg cgaaatttgc catcatttgt
       61 tcgtaaacgc tataggttgg atacctgtcc cagttagtca tttcggctac agtggttgcc
      121 atggttaatg ccttgggtat gattttagga attatttcat aaggaatatt tctgctcaaa
      181 aattcctcat attcttgttg gttagcatac gcacgcacct gatatccttg tccgccggga
      241 agctgcttca cattgtcgat agacaatatt ttagtgagat cgttgttgat ttgtgatttt
      301 tcgttaacaa caaaactaac cacgatttct ccgcgtgttt taagaatctg tgccgcttcc
      361 ggtgcctgcc cggtataata tttttgagcc gtaatgcttc ccgaaagtat tacggtgagt
      421 atgaatgtca ggtacttctt cattggttct gggaatattt taaagcgaaa aaagctgtaa
      481 ataaacggat ggttggataa caaagttaca tgtttttaaa catattaaga aagaataatc
      541 atgaaattaa atatcacgtt tagcaacatc tgaaaggttt caaaacattt ttcagaatga
      601 aatttgttgt aattaccggt gctgcacaat cattttcaaa atgtggcaac ctgatttttc
      661 cgaccctgca aattcgagtt ttagggtgta gaaaccatcc cgaagatttt tggcagagaa
      721 ctcggtttgg tttttaccac gctgacaggt ttcattcatc aatatggcaa cctgctcacc
      781 gatagcgtta aaaactttca gcgttactgt gccgttttcc ggtaggttga acagaatagt
      841 ggcgttgtcg tttaccggat tggggtgccc ctcaaaataa agagtttcct ggtggttggc
      901 atcattttgc aaaaatattt ttggcattat aaggtaagca tctgttacct cattgccata
      961 ttggtctcca ataacgcctg tgcccgatgt gatgatatct atcatgcccg gattaatgcc
     1021 atttcgtgaa ataaatccca gggtaattac cgtatcatgg gccttgatat caaaaatatt
     1081 tgtagtggca tacaccaccc tgattacgcc attatagtcg gtaaccagta aatcattgtt
     1141 ttctgcaatt atctcttcga gggatggatt aatttttgtc ggtgttcctt ttgttttatt
     1201 atttggcata accgccgaaa ccagcttata atcttccgct gaataattca gttccaggcc
     1261 taatgctgca aaatcgctta tcaggttgct gctggtcagt gaaatctcca cataggatgg
     1321 gtcgttgatt accgaggttt cttcggatga aatcaggata gtgttctttt ccagattttt
     1381 agccgtactc catgtgttga ccgaatcttt gtaactggtg cttgacgcgt tgtaatcgcc
     1441 gtatccgata gttttcagtg tgaggtttag gtttgctccg gcaacagtaa cggccgtatc
     1501 aaaagctacc cagtttcctt tcgggaaatt ggctgaagcg ctgtacggtt gtccaatttt
     1561 tgccgacatc ctggcaatgt caatagaatt tatggtggca ttattgtcaa cattggcagc
     1621 ttttaagtgt ttggccgaga atttcctcga aggatctgag gtggtgtcgt gccctacaag
     1681 gtatttcata atagccaaat caatggcatt gacatcgttg cacatttgca tggtatcggc
     1741 ggtgtacttg tcgtaagaca aaatatagtt gccattagcg acattattaa actgaaatac
     1801 tccctgcgaa ttagtggtgt cccttgccag ttcgatgccg gcaggtgatg attttaaaat
     1861 taccaggact ttgcctatat tataaatagc cggattatag gtgggcattc cgggcgcaat
     1921 tcctgcggtg gctttgctaa ggtaacgggt ttttccggtg atggagtaat tgctgcctgc
     1981 agtgatggta acattgtggt aaatggtgtc ggaacagctg ccattggtgg taattagcct
     2041 gacgttgtaa gtgccgctgg tggcataggt atgtaccggg ttttgttggg cgctggtgcc
     2101 tccgtcgccg aaattccagg catacccggt ggaatttgtg gaagtgttgg taaaagtagc
     2161 cgtatacgtg cttaccgaac tgctaaatcc tgctgttggc acggtgagaa tgctggcaac
     2221 agctggtacc cttgaactta cacaggcgtc tttttgaaca agccattcat aaaaataata
     2281 ataataagtg cttcctgcat cactgcctgt tattgacaga aggccgggtg tt
//
LOCUS       DAOSFP010000364         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_45224_length_2328_cov_4.249010, whole genome shotgun sequence.
ACCESSION   DAOSFP010000364 DAOSFP010000000
VERSION     DAOSFP010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45224_length_2328_cov_4.249010"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PLI16_09455"
     CDS             <1..448
                     /locus_tag="PLI16_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HOH84824.1"
                     /translation="PIDLNCFPHGGDFTENGYTTEEIKLENETRKILGDQSIAITATV
                     VRIPVVGGHSETVNIEFEHDFELADIVNLLSNAPGIVVQDKPEENLYPMPLTAHDKDE
                     VFVGRIRRDFSRPKSLNMWVVSDNLRKGAATNAIQIAETLTEKKFV"
     gene            448..1725
                     /locus_tag="PLI16_09460"
     CDS             448..1725
                     /locus_tag="PLI16_09460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOH84825.1"
                     /translation="MEVFKFGGASIKDAVSIKNVPVVLEHFKNKKIVVIISAMGKTTN
                     ALEQLLRSYFNKQEDTPGHLQNIKDFHFTIINELFPEENHPVYADINQLFDKLEKAIA
                     QPPDANFDYQYDRIVHFGEVISSKILWHFLNYSGIQAAWMDITNVLVTNNEFRNAKVN
                     WQKTHEQIQKHLVPVIDSNPITVLQGFIGKSETGSVVTLGREGSDYSAAIIAYCTDAE
                     SVTIWKDVDGVLNADPKFFADTQIIENISYADAVEMAYYGASVIHPKTIKPLENKNIP
                     LYVKPFANPSAAGTAIQSIPAKLPIPVNIFKTNQVLISIYPNDFSFIAEENLRDIFSL
                     FADLKISINLMQNTAISFSVVIDYHPEKLDSLIGGLQKYFKVRYNAGLELITIRNFNE
                     ATIKKILGGKKILLEQKTRYNAQFVVRNNNHQK"
     gene            complement(1800..>2328)
                     /locus_tag="PLI16_09465"
     CDS             complement(1800..>2328)
                     /locus_tag="PLI16_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mpv17/PMP22 family protein"
                     /protein_id="HOH84826.1"
                     /translation="LRIKTGNYFEKGFGIIPRAVVWAFLGATIAIAFKIFSVGTPACL
                     EYLGLSGAVDAIKGDFSALKLLTAFCISVCLNMAYAPVMMTFHKITDTHITMNHGSMK
                     SLITPIPFAKIFSGINWDVQWNFVYKKTIPLFWFPAHTITFLLPGHFQVLFAAILGVV
                     LGIILAVAAVMNKKK"
BASE COUNT          717 a          549 c          503 g          559 t
ORIGIN      
        1 tcctatcgat ttgaactgtt ttccgcacgg aggcgacttt accgaaaacg gttataccac
       61 cgaagaaata aaactagaaa acgaaacgcg taaaatactg ggcgaccaat ctatcgccat
      121 cacggccacg gtggtgagga ttccggtggt gggcggccat tccgaaaccg tgaacattga
      181 gtttgaacac gactttgaat tggccgacat agtgaacctg ctgagtaacg ccccaggtat
      241 cgtggttcag gacaaaccgg aagaaaacct ttatcccatg ccgttgacgg cacatgacaa
      301 agacgaagtt tttgtcggcc gtataagaag agatttttcc aggccgaaaa gcctgaacat
      361 gtgggtagtt tctgataatt tgcgcaaagg agcggccacc aacgccatac aaattgccga
      421 aacgttaaca gagaaaaaat tcgtttgatg gaagtattca aatttggcgg tgcttcgata
      481 aaagatgcgg tgagcataaa aaatgttcct gtcgttttgg aacatttcaa aaacaaaaag
      541 attgtcgtga tcatctcggc gatgggcaaa accaccaacg ccctcgagca gcttctccgg
      601 tcgtatttca acaagcagga agataccccg ggccacctcc agaatatcaa agatttccac
      661 tttaccatta taaatgagct gtttcccgaa gaaaaccatc ccgtgtatgc cgatatcaac
      721 caactgttcg ataagctgga aaaggctatt gcgcaacctc ctgatgccaa cttcgattat
      781 cagtacgacc gcatagtgca tttcggggaa gtaatctcgt caaaaatact ctggcatttc
      841 cttaattatt ccggaataca ggctgcatgg atggatatca ccaacgtgct ggtaaccaac
      901 aacgaatttc gcaacgccaa agtgaactgg caaaaaacac atgagcagat acaaaagcac
      961 ctggtgccgg tcattgacag taacccaata accgtgctac agggctttat cggcaaatcc
     1021 gaaaccggca gtgtggtaac cctgggccgc gaaggcagcg actattcagc cgctatcata
     1081 gcctactgta ccgatgcgga gagtgtcact atctggaaag atgtggacgg cgtgttgaat
     1141 gcagatccca aattctttgc cgacacacag ataatagaaa atatatctta tgccgatgcc
     1201 gttgaaatgg cttactacgg agcttctgtg attcatccga aaaccatcaa gcccctggaa
     1261 aacaaaaaca tcccgttgta tgtaaaaccc tttgccaatc catcagccgc cggaaccgcc
     1321 atccagagca tacccgcaaa attgcccata cctgttaata ttttcaaaac caaccaggtg
     1381 ctgatttcta tttatcccaa tgatttttca tttatagcgg aggaaaacct gcgtgatatt
     1441 ttttcactat ttgccgacct caaaatcagc atcaacctga tgcaaaacac agccataagt
     1501 ttctctgtgg taatagatta tcaccccgaa aaactcgaca gcctgatagg cgggttgcaa
     1561 aaatatttta aagtaaggta taatgcagga ctcgagctga ttactatccg taattttaat
     1621 gaggctacca ttaaaaaaat cctgggaggc aagaaaattt tactggaaca gaaaacccgg
     1681 tacaacgcac aatttgtagt cagaaataat aatcaccaaa aatagtcccc actttgtttt
     1741 cggggcatct tcaacagtac tatcctcttt tcgctactat accagccggt tgggattcgt
     1801 tatttctttt tgttcatcac tgcggccacg gccaggataa tgcctaatac gacacccaaa
     1861 atggctgcaa acagcacctg aaaatgccct ggcaacagga acgtgatggt atgtgccgga
     1921 aaccagaaca gaggaatggt ttttttgtac acaaaattcc actgcacatc ccagttaata
     1981 cccgaaaaaa tctttgcaaa aggtatgggc gttatcagcg acttcatgct gccgtggttc
     2041 atggtaatat gggtgtcggt gattttgtga aaggtcatca tcaccggtgc gtaagccata
     2101 ttaaggcaaa cgctgatgca gaatgccgtt aaaagtttta gtgccgaaaa atcgcctttt
     2161 atagcatcaa cagcacccga gaggccaaga tattcaaggc aagccggtgt ccccaccgaa
     2221 aaaattttga aggcgatggc aatagtggct ccaagaaagg cccacaccac cgcgcgcggt
     2281 atgataccga aacctttttc aaaataattg ccggtcttga tgcgcagg
//
LOCUS       DAOSFP010000365         2328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_45230_length_2328_cov_3.209855, whole genome shotgun sequence.
ACCESSION   DAOSFP010000365 DAOSFP010000000
VERSION     DAOSFP010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45230_length_2328_cov_3.209855"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PLI16_09470"
     CDS             complement(<1..278)
                     /locus_tag="PLI16_09470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOH84827.1"
                     /translation="MFEYRLSVRGYELDSYNHVNNAVYLNYYEQARWQLMKETGLLEK
                     LQNEKILVVVTEINIRYSKEARLFDNILIKSKVEFSGPYIVFKQYMFN"
     gene            complement(317..718)
                     /locus_tag="PLI16_09475"
     CDS             complement(317..718)
                     /locus_tag="PLI16_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84828.1"
                     /translation="MIKHFNNENNSEYFTEVYDFKGQWDAPSRCGLKIVENNGQTIII
                     ATELYAENPGTSVTNFCAPLATQICSEFAIDHDKVIFIVHTPDAKSKLTFLNESFFQV
                     DFAWTGSKFTDPKWSQIEKAKVDNLIQNSKS"
     gene            complement(740..2014)
                     /locus_tag="PLI16_09480"
     CDS             complement(740..2014)
                     /locus_tag="PLI16_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225159.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOH84829.1"
                     /translation="MQRIKFKLIYPKWEKLEGQTTFNLPPHGPVVMAAALPDYVDVEF
                     TDENVDEVIFNNDVDFVGISMMLTSQIKRGWEIATEYRKRGKKVIFGGISTMLHAEET
                     LEYADSIFLGEAEGRMEKVFEDFANNKLQKVYNYLTVHPDINTIGPARRSILNTEKYY
                     HKGVRMVDLFHASRGCKYNCYPCAVAYLGGKCFRPRPIDKVVEELATIDNNRLFIVDN
                     SLAQDKQWELDLFREMIPFKKNWCSHPIEDDDEVLDMAAQAGAWFVYQAVFDTSDYIK
                     NRIKRYHDHGIAVEGTILLGTDDHTEDDIKRLIDFLLEVNLDLAEFTVLTPFPHTKAY
                     DDMLRQNRIIDKDWNNYNAGKVVIQPKQMTPEKLQELYHYAWDTFYKDETQSEKMFKL
                     FHSVIMKEIANGTYKPIREELSRSSFGKKVIR"
BASE COUNT          582 a          454 c          524 g          768 t
ORIGIN      
        1 tcgttgaaca tatactgttt aaagacgatg tatggccccg aaaattccac cttgcttttg
       61 atgagaatat tgtcgaacaa acgggcttct ttggaatacc tgatattgat ttcggtaact
      121 accaccagaa ttttttcgtt ctgtagtttt tcgaggaggc cggtttcttt cataagctgc
      181 cagcgggcct gttcgtagta gttcaggtaa acggcattgt taacatggtt gtatgaatcc
      241 aattcatagc cacgtaccga taatcgatat tcaaacataa tttaatttat gaattcggta
      301 atgcgatttt tccgcatcag gatttgctgt tttgtattaa attatcaact tttgcttttt
      361 ctatctggct ccattttggg tcggtaaatt tggagccggt ccatgcaaag tcaacctgaa
      421 aaaaactttc atttaaaaat gtcagttttg atttagcatc cggtgtgtgc acgataaaaa
      481 ttaccttgtc atggtcaatg gcaaactcgg agcatatttg tgttgccagg ggtgcgcaga
      541 aatttgttac cgaggtgccg gggttttcag cgtagagttc ggtggcaatg ataatggttt
      601 gcccgttgtt ttccacaatt ttaagtccgc agcgtgaggg ggcatcccat tggcctttga
      661 aatcgtatac ttcagtaaaa tattcagaat tattttcgtt gttgaaatgc ttaatcataa
      721 taccaggtga gctttttgat tatctaatta cttttttccc aaaactgctt cttgataatt
      781 cttcgcgtat gggtttgtag gtgccattgg cgatttcttt catgatgacc gagtggaaaa
      841 gcttaaacat tttttccgac tgggtttcat ctttgtaaaa agtatcccat gcataatggt
      901 acaattcctg gagtttttca ggagtcatct gtttaggctg aatgaccact ttaccggcgt
      961 tgtaattatt ccagtcctta tcgatgatgc ggttttgcct gagcatgtcg tcgtaagctt
     1021 tggtgtgtgg gaagggcgta agtacggtaa attcggccag gtcgaggttg acttcgagca
     1081 ggaagtcgat cagccgttta atgtcgtctt cagtatggtc gtcggttccc agtaggatag
     1141 tgccttctac cgcgatgcca tggtcgtggt aacgttttat tctgtttttg atatagtccg
     1201 aagtatcgaa tacggcctga tatacaaacc atgctccggc ttgtgccgcc atgtcgagta
     1261 cttcatcgtc gtcttcaata gggtgggagc accagttttt cttgaagggg atcatttcgc
     1321 ggaagaggtc gagttcccat tgtttatcct gtgccagtga gttgtccacg ataaacagcc
     1381 tgttgttatc gatggtggcc agttcttcaa ccaccttgtc gatggggcgc ggcctgaagc
     1441 attttcctcc gaggtaggct acagcgcaag ggtagcagtt gtatttgcat ccacgcgaag
     1501 catggaaaag gtccaccata cgcactcctt tgtggtagta tttttccgtg ttcagaatac
     1561 ttctgcgggc agggccgatg gtattgatat cagggtgtac ggtgaggtaa ttataaactt
     1621 tctgcaattt attgttggca aaatcctcaa aaactttttc catacgtcct tcggcttcac
     1681 caagaaatat tgaatcggca tattccagag tttcctcggc atgcagcatg gtggatatcc
     1741 cgccaaaaat cacttttttg ccccgcttac ggtattcggt ggcaatttcc catccgcgtt
     1801 ttatctggct cgttagcatc atagaaatgc caacaaagtc aacatcattg ttaaaaataa
     1861 cttcgtctac attttcgtcc gtaaattcca catcaacata atcgggtaag gcggcagcca
     1921 taactaccgg gccatgtggt ggcagattaa aagtggtttg tccttcgagt ttttcccatt
     1981 tagggtagat aagtttgaat tttatgcgtt gcatggatta gaatttttct tagtttatta
     2041 ataatctgtt taagtatcca aatgatgtag tcatctgctt gtaaaacgga gtgcaaagat
     2101 aagtcaattt gaaataactt ttgttgctat gcatatattg tcatagataa aaaatgttgt
     2161 tttgaagaca tttatttttt gaaaggcttt atgttgccgc tgaggacaca ttcagcagac
     2221 atactcaggt gttattaatt ttttccacca gatatttcat tccaagcagt tcggcacagg
     2281 tagttaccga gccaatagta acgcccagta cgccgtggag aataatgc
//
LOCUS       DAOSFP010000366         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_45247_length_2327_cov_3.065141, whole genome shotgun sequence.
ACCESSION   DAOSFP010000366 DAOSFP010000000
VERSION     DAOSFP010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45247_length_2327_cov_3.065141"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            488..877
                     /locus_tag="PLI16_09485"
     CDS             488..877
                     /locus_tag="PLI16_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84830.1"
                     /translation="MATPPVIIYKTYKDYHKYVPVTLSADKSAIVSYPDIKDVYYNDT
                     LAYPTRLARGFWLDNRGIGPHAAFLKFTYEEYSQLEATPAPDVLFQNILETDPFKEIY
                     KLSCERDTVEINKIIKSGLSENCKKIK"
     gene            929..2041
                     /locus_tag="PLI16_09490"
     CDS             929..2041
                     /locus_tag="PLI16_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOH84831.1"
                     /translation="MKKLLYLSFIIALLGAGNIYAQKKAVIKFDKTVHDYGKIKEEAG
                     KVKCRFTFTNTGNDTLKIINVKPSCGCTAAEWTKTGVLPKKTGFIDAVFDPDHRPGPF
                     DKGIAVTTNAPDNPMINLVIKGEVLPKPKEMTDRYPVVVGNMRMETSQFNFGNTKNTE
                     IRTDTLNIINVWNRPMTLSFKDFPPYLTAKAIPETLKPNKEGIIILDYDVAKRNEWGY
                     VFDRLMLYTNDSLQPNKTINISQNITEDFANMTAEQMAVAPKIKWDNTTFDFGTAKQG
                     DKVECTFVFRNEGENDLIIRKVKASCGCTATNPEKTVLKKGEESKINATFNTAGKSGK
                     QYKSITIISNDPVNSTTMLSIQGTLDQPAPAETPQK"
BASE COUNT          798 a          503 c          427 g          599 t
ORIGIN      
        1 ccggccagaa agccttaacc actaaatgga gcatgaccag ggaatctttt cctccggaaa
       61 acaacaacac ggggtttgta aactgagcgg cagcctcgcg gataacaaag atggcctccg
      121 attcaagatc gcttatatgg gttgaatgat taatcataaa aacataaaca ttaccttctg
      181 ctttcagtaa tcattttttt tagcaaaagg atattcgtcg acatcatgac aataatattg
      241 aattcccgcc gaacttggta tattctttgc taaaaaaaat gcaaatgtag catttattat
      301 catttgctgt tttttactta gtaaaattat caagtacaca aaccgtgcta actatatgaa
      361 tattaggaaa atattttttt tactgatccc gctttctatg tttttgctaa cttcgtgtaa
      421 aactgcaaag gaaaagaata ctgcagataa tacctctgaa actaatcatg acaccaatac
      481 tatggttatg gccactcccc cggttattat ttacaaaacg tataaagact atcacaagta
      541 cgttccggta acattgtcag ccgacaaatc tgccattgtg tcgtaccctg acataaaaga
      601 tgtttattat aatgacacac tggcctatcc caccaggctg gccagaggtt tctggctcga
      661 taaccgggga ataggaccac acgctgcttt tttgaaattt acctatgaag aatacagtca
      721 gctggaggcc actcccgcac cggatgtgct gtttcaaaac attttagaaa ccgacccctt
      781 taaagaaata tacaaattat cctgcgagcg ggacacggta gaaattaata aaataataaa
      841 atcgggactg agcgaaaact gcaaaaaaat aaaataaaca caaaccactt acgttttata
      901 atgtattttt acaaaaaatt tttatcgtat gaaaaagtta ttatatctga gttttattat
      961 cgcactgctg ggtgccggaa acatttatgc ccagaaaaag gctgtcataa aatttgacaa
     1021 gaccgtacat gattatggaa agatcaaaga agaagccggt aaggtaaagt gccgctttac
     1081 ttttaccaat accggcaacg atacgttgaa aattatcaac gtaaaacctt cgtgtggatg
     1141 taccgccgcg gaatggacca aaacaggggt attacccaaa aagacaggtt ttattgacgc
     1201 ggtatttgat ccggaccacc gtccaggccc gttcgacaaa ggtattgccg ttaccaccaa
     1261 cgcacccgac aacccgatga taaacctggt gattaagggc gaggtattac ccaaacctaa
     1321 agaaatgacc gaccgctatc ctgtggtggt tggcaatatg cgcatggaaa cttcacaatt
     1381 taatttcggg aacacaaaaa acaccgaaat acgtaccgac acgctgaata tcatcaatgt
     1441 gtggaacagg ccgatgacct taagcttcaa ggattttccg ccctacctga ctgcaaaagc
     1501 tatccccgaa acacttaaac ctaataaaga gggtatcatt attctggatt atgatgtagc
     1561 aaaacgcaac gaatggggtt atgtgttcga caggttaatg ctgtacacca acgacagcct
     1621 gcaaccaaac aaaacgatca acatcagcca gaacatcacc gaagatttcg ccaatatgac
     1681 tgcagaacaa atggccgttg ctcccaaaat aaaatgggac aacaccactt ttgatttcgg
     1741 taccgccaaa cagggcgaca aagttgaatg tacctttgta ttccgtaacg aaggagaaaa
     1801 cgacctgatc atccgcaagg taaaagccag ttgcggctgt acagccacca accccgaaaa
     1861 aaccgtcctg aaaaaaggcg aagaaagtaa aataaacgcc acttttaata ccgcaggaaa
     1921 atcaggcaaa caatacaaaa gtatcaccat catttctaac gaccctgtaa actccaccac
     1981 gatgctgagt attcagggca ctctggatca acctgctcct gcagagactc ctcaaaaata
     2041 agacattaat taatccacaa aagccctgaa cccctttgtt cagggctttt tttatcccgg
     2101 atcccgtaca aagtattctc taatactaaa catcaaataa ttgggataac aaaaatttta
     2161 aatgacaggg ttttatccat catttttata gtaattttat cttttaaaaa gaaatttttt
     2221 cgtataaaga tttctgtaat gaacgaaagg tgtggctatg agcgactcaa aaaatgattc
     2281 tttcgaaaaa tcacgcaaag tagctgcccg acggtggttt caaattt
//
LOCUS       DAOSFP010000367         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_45260_length_2326_cov_3.774989, whole genome shotgun sequence.
ACCESSION   DAOSFP010000367 DAOSFP010000000
VERSION     DAOSFP010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45260_length_2326_cov_3.774989"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="PLI16_09495"
     CDS             <1..827
                     /locus_tag="PLI16_09495"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOH84832.1"
                     /translation="DNSVTIQEFMIMPVGAENFTEAIRMGAEIYHKLRDVLKKAGYST
                     NVGDEGGFAPMLKSNEEALQVIIKAIENANYKPGVDVFIALDPAASEYYDDNEKVYHL
                     KKSTGEKLNSNEMVDFWKDWCNRYPIISIEDGMAEDDWKGWALITKTLGSKIQLVGDD
                     LFVTNTDRLQMGIDKKCANSILIKVNQIGTLTETINAVNLAYLNGYTAIISHRSGETE
                     DTTIADLAVALNTGMIKTGSACRTDRICKYNQLIRIEEMLYGTGKYLGKDFKFLKK"
     gene            complement(1110..>2326)
                     /locus_tag="PLI16_09500"
     CDS             complement(1110..>2326)
                     /locus_tag="PLI16_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84833.1"
                     /translation="HIWGDDGGTCTGSDYCTDVPNQANYTYGVHTGVLTDKCTAKSPG
                     IMYMNFMDYSDDISYANFTPNQKARIAALFASGGLLYSLSVSDGCTAPGTIACGTPSG
                     LSATGITTTSATLNWAAVSGASSYNIQYRPSGTSTFVTTTSTTNSKAITGLTDGTIYE
                     FMVQAVCSETGTYSGLSSFTTLTAGGCTDIYESNNTVATAKTIAVNTNINASIETAAD
                     VDYYKFSNTSAKKKIKVTLTDLPADYDLKLFKSNGTTLIATSQNSGTTTESIVYNNAK
                     VGTYVIKVYGYNGAYSASSCYTLTASISSLSWKGVEEVMNEPDTPQDIMIYPNPASEN
                     LNVTYNAENNGDVQISIYDLAGRLLNSTSYQANKGLNNYSMSVSALAKGIYVLSLKNG
                     NTLLNQKLIIDK"
BASE COUNT          657 a          460 c          542 g          667 t
ORIGIN      
        1 ctgacaacag cgtaaccatt caggaattca tgattatgcc ggtaggtgcc gaaaacttta
       61 cagaagccat cagaatgggt gccgaaatat accacaaact ccgggatgtt ttgaaaaaag
      121 caggttattc aactaatgta ggtgatgaag gcggtttcgc acccatgctg aaatctaacg
      181 aggaagcact gcaggtaatt atcaaagcca ttgaaaacgc taattacaag ccgggcgtag
      241 atgtttttat cgccctcgat ccggcagctt cagagtatta tgatgataat gaaaaggtgt
      301 accatctgaa aaaatctacc ggcgaaaaac ttaattccaa cgaaatggtg gatttttgga
      361 aagattggtg taacagatat cctatcattt caatcgaaga cggcatggcc gaagacgatt
      421 ggaaagggtg ggcgctgatt acgaaaacgt taggcagtaa aattcaatta gttggtgatg
      481 atctttttgt taccaacacc gaccgtctgc aaatgggtat cgataaaaaa tgtgccaatt
      541 ccattttaat taaagttaac cagataggaa ctctcaccga aactattaat gccgtcaatt
      601 tagcctatct taacggatat actgcaatta tcagccatcg ttcgggagaa actgaagata
      661 ctacaatcgc tgacctggcc gtcgccctca ataccggaat gataaaaacg ggttcagcct
      721 gtcggaccga tcggatatgt aaatataacc agctgatacg aattgaagag atgctttatg
      781 gtacaggaaa atacctcgga aaagatttta aatttctgaa gaagtaactc ttgttatagt
      841 attgtctgcc ttgacaatgc cctgtcagga aagaggctgt ctcaaaaaga cagcctcttt
      901 aatttgtaat tcgaaagaac ccgtcataat ggctatttgt gggtgttttg cagatttact
      961 acgctttttg aatccccaac ctggctttga taataaatca tacacaacag tttaatattc
     1021 cctcaaaaga aaactgacct taacgctgaa aaacaaaaaa ggccgcctcg attatgagac
     1081 agcctttttg gtttttttta cttccagcct tatttgtcga taataagttt ttggttgagc
     1141 agggtgttgc cgtttttcaa cgaaagaaca tagatgcctt tcgcaagtgc tgacacgctc
     1201 atcgaataat tattaaggcc tttgtttgcc tgatagctgg tagaatttaa aaggcgtccg
     1261 gcaaggtcgt aaatactaat ctgcacatca ccattatttt ctgcattata ggtaacattc
     1321 aggttttcgc tggcagggtt ggggtaaatc ataatgtcct ggggggtatc aggttcattc
     1381 atcacttctt ccacgccttt ccatgaaagg ctgctgatgc tggctgtcag tgtatagcaa
     1441 ctgcttgcgg aataagcgcc attgtaccca tagactttta taacgtatgt tcccaccttg
     1501 gcattgttat aaacgatact ttcggttgtg gttcctgagt tctgcgaggt ggcaattaga
     1561 gtagtcccgt ttgatttgaa gagtttcaag tcgtaatcgg caggtaagtc tgtcagggta
     1621 actttaattt ttttcttcgc cgaagtattg gagaatttat aataatcaac atcggcagcg
     1681 gtttctattg atgcattgat attggtgttg acggcaattg tctttgctgt ggcaaccgta
     1741 ttgtttgatt cataaatatc ggtacagccg cctgcggtca gcgtggtaaa tgaggacagt
     1801 cctgaataag tgcctgtttc agaacatact gcctgcacca taaattcata aatagtgccg
     1861 tctgtcaacc ctgtgatggc tttggaattg gtggtggaag ttgtggtaac aaaggttgat
     1921 gttcctgatg gacgatactg aatattgtag ctggaagcgc cggatacggc tgcccagttg
     1981 agggttgccg atgtggtggt aataccggtg gctgataacc ctgagggcgt gccgcaagcg
     2041 attgtgccgg gggctgtgca gccgtctgaa accgataagg aatataatag tccgccggag
     2101 gcaaaaagag cagctatacg agccttttgg tttggggtga aattggcata tgaaatatca
     2161 tcgctgtaat ccataaaatt catatacatg attccaggcg attttgccgt gcacttgtcg
     2221 gtcaatactc cggtatgcac accatacgtg taattggcct ggttgggaac atccgtgcaa
     2281 tagtcgctgc ctgtgcacgt accgccatca tcgccccaaa tatggt
//
LOCUS       DAOSFP010000368         2311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_45680_length_2311_cov_3.291667, whole genome shotgun sequence.
ACCESSION   DAOSFP010000368 DAOSFP010000000
VERSION     DAOSFP010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45680_length_2311_cov_3.291667"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLI16_09505"
     CDS             <1..511
                     /locus_tag="PLI16_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84834.1"
                     /translation="RYSDRVFFGTGPALIKGVQSDWNSYPMYAYQLFDEVKHTFHLFE
                     DLFSADVPTPMDTFLEKTFLTKNIWVPLRENFKEKNKFIRACAEKVDGLRILQFLKSR
                     QSVSENEMRYIVEYLKLFHHAEAEEILHAASDLHFDTSPVEILDKLRDLLSNIENNYR
                     KKNQVINWF"
     gene            complement(617..928)
                     /locus_tag="PLI16_09510"
     CDS             complement(617..928)
                     /locus_tag="PLI16_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOH84835.1"
                     /translation="MKIYAGNLHYGMTEDELQKIFEEYGQVASVKIITDKYSGRSKGF
                     GFVEMPDDGEAQQAIEDLNGKDVKGRNITVNQSIERKEGEHRNNRRDNFRRNNNYRNN
                     D"
     gene            complement(1207..1398)
                     /locus_tag="PLI16_09515"
     CDS             complement(1207..1398)
                     /locus_tag="PLI16_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84836.1"
                     /translation="MKTTKIKILLTLVFLSVPLFITEVLAQAPPPAPSGNSVPINGLG
                     ILAALGIGYGIKKLRGKNK"
     gene            complement(1438..>2311)
                     /locus_tag="PLI16_09520"
     CDS             complement(1438..>2311)
                     /locus_tag="PLI16_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84837.1"
                     /translation="NSHYYGTYSSYVGGYSTNGGTNIIPSMQGFFVKANASTGSLGVN
                     NAARLNAQPTFFKSTNTSNPLLRLRGYPTQNSAHSDETIIYFDQNATAMFDGAYDAYK
                     LMNNDPAFPNIFTKDSSVYTLSINALPPLSAADVVIPLGFSTKTNGSFTINASEILNF
                     DPSQHVYLEDNQTSTIQDLTLNPVYTFTTTANALQYRFFIRFSPTIVTGIDENNTSFV
                     DAWSSGKDIYVNYSNTTLQKAVISVYDMLGQQVISDEVQGSSTFRYTVGKPGCYIINV
                     IGGGNSFQKKIIIL"
BASE COUNT          663 a          441 c          481 g          726 t
ORIGIN      
        1 caggtattcc gacagggtgt ttttcggcac cggccctgcc cttattaaag gtgtgcaaag
       61 cgattggaac agttacccaa tgtatgccta tcaactgttt gatgaggtaa aacacacatt
      121 ccatcttttt gaagacctct tttctgccga tgtaccaacg ccaatggaca cttttctcga
      181 aaagacattt ctgaccaaaa atatttgggt gccgttgcgg gaaaacttta aggaaaaaaa
      241 taaatttatc agggcctgtg ccgaaaaagt tgacgggtta aggatattgc aatttcttaa
      301 atcaaggcaa agtgtctccg aaaacgaaat gagatatatt gtggaatatt taaagctttt
      361 tcaccatgca gaagcagaag aaatattaca tgccgcgagc gatttgcatt tcgacacatc
      421 tcctgtagaa atactggata aacttaggga tttgctctcg aacatagaaa ataactaccg
      481 caaaaaaaac caggtgataa actggttcta atgatacggt acagtaatgt ttatgcaaaa
      541 aaaacagcga cctgatggcc gctgtttatg cttttaaaaa attttcgaaa caggaatttg
      601 tttctaagaa aaataattaa tcgttattcc tgtaattatt atttctcctg aaattatctc
      661 ttctgttgtt tctgtgttct ccttcttttc tttcgattga ttgattaaca gtaatgtttc
      721 tgccttttac atccttgcca ttgagatctt caatagcttg ctgcgcttca ccatcatcag
      781 gcatttcaac aaaaccaaat cctttgcttc ttcctgagta tttgtcagta atgattttaa
      841 cagaggctac ttgtccatac tcttcaaaaa ttttttgtaa ttcatcttca gtcattccgt
      901 aatgaagatt accagcgtaa attttcataa tgcgataatt aattttaatt ggttattaac
      961 gtacaaaagt acagtatttt tttacatcag cgaaaaatat taatatttat ttgttaaaat
     1021 ttggggtatt tgataatttt atttttaaaa cggatcaatt catgattgtt tttgatgggt
     1081 tgttgtcctt ttccagagga ggtaaacaat agccaaacct tccttcagca aagggaaagc
     1141 gaaatcggtt gacctcctgc ccgagcaaca gggcgattga gtatgctgca acagaatgtt
     1201 tatttttcac ttattcttac ccctgagttt ctttattcca tagcctatac ccagggctgc
     1261 cagtatgccc aaaccattta tgggcacact gttgcccgag ggcgccggag gcggcgcctg
     1321 ggccagcact tcagtaataa ataagggaac cgacagaaaa accagggtca gcagtatttt
     1381 tatctttgtt gttttcatgt ttttcgcttt ttagtttttt attactacca ttactgctta
     1441 caagatgatg atttttttct ggaaactgtt tccaccgcca atcacgttga tgatgtaaca
     1501 tccgggctta cccaccgtgt aacggaaggt gctgctgccc tgcacttcat cgctgatgac
     1561 ctgttgaccg agcatatcat agacagatat tactgccttt tgcagtgtgg tgttgctgta
     1621 attaacataa atgtccttac ccgatgacca tgcatcaaca aaggaggtgt tgttttcatc
     1681 aatgccggta acaatagtcg gcgaaaatct aataaagaac ctgtattgca gggcattggc
     1741 agtggtggta aatgtataaa ccggatttaa tgtcaggtcc tgaatggtgg atgtctggtt
     1801 atcttcaagg tatacatgct gcgaaggatc aaagttgagg atctcgctgg cattaatggt
     1861 aaatgagcca ttggttttgg tcgaaaaacc aagaggtatt accacatcag cagcagaaag
     1921 cggtggcaaa gcgtttatag ataaggtata taccgagctg tcctttgtga agatattagg
     1981 aaatgcaggg tcgttgttca tcaacttata ggcatcgtac gccccgtcga acatggcagt
     2041 ggcattctga tcgaaataaa tgattgtttc gtcgctgtgt gcgctgtttt gcgtgggata
     2101 gcccctaagc cgcaataaag gatttgaggt gtttgtggac ttaaagaaag tgggttgcgc
     2161 gtttagcctt gcagcattat taactcccag tgagcctgtg gaagcattag cttttacaaa
     2221 gaagccctgc atggaaggaa tgatattggt accgccatta gtactatatc cgcccacata
     2281 agaactgtag gtgccataat agtgtgaatt g
//
LOCUS       DAOSFP010000369         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_46117_length_2296_cov_4.396252, whole genome shotgun sequence.
ACCESSION   DAOSFP010000369 DAOSFP010000000
VERSION     DAOSFP010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46117_length_2296_cov_4.396252"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /gene="msrA"
                     /locus_tag="PLI16_09525"
     CDS             complement(<1..393)
                     /gene="msrA"
                     /locus_tag="PLI16_09525"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOH84838.1"
                     /translation="MEHKTNLDTATFGGGCFWCIEAVFEQVAGVKQVVSGYAGGETKN
                     PTYGQVCSGETGHAEVCQVVFDPEIISYKELLLIFFASHDPTTPDRQGPDFGSQYRSV
                     IFYHDEQQEITAKNLIKIFYDSSMPRRRW"
     gene            complement(533..1579)
                     /locus_tag="PLI16_09530"
     CDS             complement(533..1579)
                     /locus_tag="PLI16_09530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84839.1"
                     /translation="MKIKPLYQIFALLLILFTGIQAYSQKISGTPVSLPKQDNLANGK
                     TPNDTLLPGNLANLATPEYVMIPSDNGGYVLGTNGWNDKCKCQQFKVSYYYHIEGAIY
                     WFGYKRADSSGLIRFAIWNMDSINGTTYDTTDTINQIHTGQLSPGTLFVAITDTIKNV
                     DTSADMSHAHIVMFPFPVLVTSDYCLGFDITNSGADSIALVSTNKGQGGNLQLVWEQW
                     SSNNLWYTLQGAQWDSSKLDIDGMIFPIIDNTVGCVEQGDFISGIKMSQSYPNPNPGY
                     CTINYELESPQNKVSLMILGMDGKQVFYSESLETTSGIHQQTIDVSHLANGTYFYVLS
                     AGSAHLAKKMIISK"
     gene            1750..2274
                     /locus_tag="PLI16_09535"
     CDS             1750..2274
                     /locus_tag="PLI16_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH84840.1"
                     /translation="MKVEKNKMVSLTYRLTENDAQGILIEETKAEQPFDFLFGVDGVI
                     PGFEDNLRNLQVDDAFAFAVEAKDAYGAYDDNGVVELPITVFQKDGKLDTNICVVGNM
                     IPLRGENGQPFYGIVKEIKESVVVMDFNHPLAGKNLFFSGRIIGVRDASEEEICQRQG
                     CNHEGHDCSSGCSC"
BASE COUNT          653 a          457 c          514 g          672 t
ORIGIN      
        1 ccatcgccta cggggcatcg aggaatcata aaatatttta attaaatttt ttgcagtgat
       61 ttcttgctgt tcatcatgat agaaaataac cgagcggtat tgtgaaccaa aatccggacc
      121 ctgacggtcg ggcgtggtag ggtcgtgtga agcgaaaaat atcaacaaca attctttata
      181 gcttatgatt tcagggtcga aaaccacctg gcaaacctct gcatgccctg tttcgcctga
      241 acaaacctgt ccataggtcg gattttttgt ttcgccgccg gcatagcccg aaactacctg
      301 tttaacgccg gccacctgtt caaaaactgc ttcgatacac caaaagcagc caccaccaaa
      361 tgttgctgtg tcaagatttg tcttatgttc catacactct ttttttacaa tttcagcagc
      421 ctgccccggt aagacataaa acaagacact taaggcaatg actgcgcgaa tcagcataat
      481 aaatcaatac agtatttttg taataacgca gacgagagca aaaaagtact ccttacttgc
      541 tgataatcat ttttttggca agatgagcgg atccggcaga aagtacataa aagtaggtgc
      601 cgtttgccag atgcgaaaca tcaatcgttt gttgatgaat gcctgatgtt gtttcaagtg
      661 attcgctgta aaatacctgt ttgccatcca tgccgagtat catcaggctg accttatttt
      721 gcggcgattc cagctcatag tttatcgtgc agtatccggg attgggatta ggatacgact
      781 gactcatttt tattcccgaa ataaaatcac cttgttccac acacccaacc gtattatcta
      841 ttatgggaaa tatcatgccg tcgatgtcca gtttgctgct gtcccattgg gcgccctgga
      901 gggtgtacca aaggttgttt gaactccatt gctcccatac cagttgcagg ttgcctccct
      961 ggcctttgtt ggtgctaacc agggcaatgg aatctgcgcc cgaattggtg atgtcaaatc
     1021 ccaggcaata atccgatgta actaatacgg ggaacggaaa catcacaata tgtgcatgac
     1081 tcatgtcggc ggaagtgtcc acatttttta tagtatcggt aatggctaca aacagtgtcc
     1141 cgggacttag ctggcctgta tgaatctggt ttatggtgtc ggtggtgtca taagtggtgc
     1201 cgttgatgct atccatattc cagatcgcaa aacggataag gcctgaggaa tcggcgcgct
     1261 tatagccaaa ccaatagatg gcaccttcaa tatgataata ataggagact ttaaactgct
     1321 gacatttgca tttatcattc cagccatttg ttcccaaaac atatccgccg ttatccgatg
     1381 gaatcatcac atattcaggg gtggcaagat ttgccaggtt gccgggcaac aaggtgtcgt
     1441 tgggcgtttt tccattggcc aaattgtctt gtttgggtaa tgataccgga gttcctgata
     1501 ttttttgcga ataggcctgt atgccggtaa ataaaatcag caacagggca aatatttggt
     1561 atagaggttt gattttcata gggggcaaag gtactaatat tttcagagca aatgaataat
     1621 tgttcgggtt aatacatagt tgcaaaacga aaaactattc gccaaagtat ttgttttgaa
     1681 tagttacgga tgattttttg caatcgccat gatattaaac cttacctttg cggcctaaaa
     1741 actattaaaa tgaaagtaga aaagaacaaa atggtttccc ttacttaccg gcttacagaa
     1801 aatgacgccc aaggcatatt gattgaagaa acaaaggcag aacagccttt tgatttcctt
     1861 tttggcgttg atggtgtcat acccggattt gaagacaatc tgcgcaatct gcaggttgat
     1921 gatgcttttg cttttgctgt ggaagcaaaa gatgcttatg gtgcatacga cgataacgga
     1981 gtggttgaat tacctattac cgtatttcaa aaagatggca aattagacac caatatttgt
     2041 gtggtgggca atatgattcc tctgcgtggc gaaaatggtc agccatttta tggaattgtc
     2101 aaagaaataa aagaatctgt tgtggttatg gattttaacc accctctggc tgggaaaaat
     2161 ttgtttttta gcggcaggat tattggcgtt cgcgatgctt ctgaagaaga aatttgtcag
     2221 cgtcagggat gcaaccatga aggacacgat tgttcgagtg gttgtagctg ctaaaggtta
     2281 ttttacataa taaaaa
//
LOCUS       DAOSFP010000370         2278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_46623_length_2278_cov_4.136752, whole genome shotgun sequence.
ACCESSION   DAOSFP010000370 DAOSFP010000000
VERSION     DAOSFP010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46623_length_2278_cov_4.136752"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLI16_09540"
     CDS             complement(<1..712)
                     /locus_tag="PLI16_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84841.1"
                     /translation="MLHYKKLLLLSLLLLLIVFTCPGQHIAAVQEPETFSGGSSVQVN
                     VFFNCGIWRDSIVHMVWENHDANNIAQVFVESSYDGNNFSVISGATITNLIDIHLYNY
                     PKEIDYNKQVLMSSEHGNIRFIYNDVIMDPVAIHSRWYRIRMVTFTGATYISQVIHSD
                     DDGQKVITTNSNPIIIDNTPEDIKALHKGVKVACPSIGTPPSGYIATAQTQSFFGDCC
                     QWEERLYQGTGPIYTTCSG"
     gene            865..1521
                     /locus_tag="PLI16_09545"
     CDS             865..1521
                     /locus_tag="PLI16_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOH84842.1"
                     /translation="MINTIITFLENLSANNNREWFEKHKSSYEIARNNFYSIITALHK
                     EISVFDEGVRMLNPKDCIFRIYRDMRFSKDKTPYKTNFGAYMNLFGKKVNNAGYYFHL
                     EPGNCFLCGGIYMPQAPVLKLVRSEIYYNFDEFKKIISEKKFREFFGDVSGDRLQKIP
                     AGYPRDFAGANYLLLKDFLALHQYNPLQLGEKELVPYVINVFKAMKPLNDFLNKSLEN
                     "
     gene            1587..>2278
                     /locus_tag="PLI16_09550"
     CDS             1587..>2278
                     /locus_tag="PLI16_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOH84843.1"
                     /translation="MPVRAEPSDTSEIVSQLLFGELFRLTETRRSWVKICMTYDNYEG
                     WIDKKQCCLLTDEEFQRLHNDVKAVATDTISIIRDVTLGLDYPIVSGSSLPGLDEKKL
                     YIAGHEYFFDAVYRDVSVKADRGFASDLAFTYLNAPYLWGGRSPLGIDCSGFTQVVMK
                     MCGIALPRDAAQQAMEGVPVDFVEQALPGDLAFFENAENRIIHTGIILSGQRIIHASG
                     KVRLDDIDHEGI"
BASE COUNT          662 a          429 c          496 g          691 t
ORIGIN      
        1 tgcccgaaca ggtagtatag ataggacctg tgccctgata tagtctctct tcccattgac
       61 agcaatcccc gaagaaactt tgtgtctgag cagtggctat atagccagaa ggcggtgtgc
      121 ctatggaagg acaggccact tttacgccct tatgcaaggc tttaatatct tcaggggtat
      181 tgtcgataat tatggggttg ctgttggttg taataacttt ttgtccatcg tcgtcagaat
      241 gtattacttg agaaatgtac gtggctcccg taaacgtaac catcctgatc cggtaccatc
      301 tggaatgaat ggccaccgga tccattatca catcattgta aataaatctg atgttgccgt
      361 gctccgatga cattaaaacc tgtttgttat agtctatttc ctttggataa ttgtataaat
      421 gaatatctat cagattggta atggtagcgc cagaaatcac agaaaaatta ttgccatcat
      481 agctcgattc tacaaacacc tgggcgatgt tgtttgcatc atggttttcc caaaccatgt
      541 gcacgatgct gtcacgccag atgccgcagt tgaaaaaaac atttacctgt acggatgacc
      601 ctcccgaaaa cgtttcaggc tcctgcaccg cagcaatgtg ttggccgggg caggtgaaaa
      661 caataagcag gagtaatagc gagagcagga gtaatttttt ataatgtaac ataatttctt
      721 atgattttat cataccatat tatataacgg caaaaaaata tttatacttg ttagtagcac
      781 atatttttat tcgaatgttt tttttataga agtaatttgt aaaaataata aataaatttg
      841 ttcgtttttt atcttttcat aaaaatgatt aacacaatta ttactttttt agaaaatttg
      901 tcggcaaata ataatcgtga gtggtttgaa aaacataaat catcttatga aattgcccgt
      961 aacaattttt attctataat aactgccttg cacaaggaaa tctcagtgtt tgatgaaggt
     1021 gtgaggatgc tgaaccccaa agactgtatc ttcagaatat accgcgatat gaggttttca
     1081 aaggacaaaa ctccttataa gacaaatttt ggcgcataca tgaatctgtt tgggaaaaag
     1141 gtcaataatg ccggatatta ttttcatctg gagcctggca attgtttcct ttgcggaggc
     1201 atttacatgc cgcaggcacc cgttctgaaa ttggtgcgca gcgaaattta ttataatttc
     1261 gacgaattca aaaaaatcat ctctgaaaag aaattcaggg agttctttgg tgatgtatca
     1321 ggcgataggt tgcaaaagat accggcgggt tatccacgcg actttgcggg agccaattat
     1381 ctcctgttga aagatttctt agccctgcac caatacaacc cgctgcaact tggcgaaaag
     1441 gaactggtgc cttatgttat caacgttttt aaggcgatga aacccttaaa cgattttctg
     1501 aacaagtcat tggaaaattg aaaataatta gcgggaaaat attaaaataa ttaaatatgc
     1561 accagggaat atgtgtgtta agtgttatgc cagtgcgagc cgaaccttcc gatacttccg
     1621 aaatagtttc gcagctattg tttggagaat tattccggct caccgaaacc cgccgttcgt
     1681 gggttaaaat ctgcatgact tatgataatt atgaaggctg gattgacaaa aaacaatgct
     1741 gcctgcttac ggatgaagag tttcaaagac tccataacga tgtcaaagcg gtggctactg
     1801 atactatttc cattattcgt gatgtgactc ttggattgga ttatcccata gtgtcaggta
     1861 gcagtttgcc cggcttagat gaaaaaaagc tttatatcgc tggacatgaa tatttctttg
     1921 atgccgtgta tagggatgtt tcggtaaagg cggatagggg atttgccagt gatcttgctt
     1981 tcacctatct taatgctcct tatctatggg gtggcagatc tcctctgggc atcgactgtt
     2041 cgggctttac acaggtagtg atgaaaatgt gcgggattgc attgcctcgc gatgctgcgc
     2101 aacaggccat ggaaggagtt cctgtggatt ttgttgaaca ggcgctgccc ggcgacctcg
     2161 ctttctttga aaacgccgaa aacaggatta ttcacacagg aattatactt tcaggccaga
     2221 gaattattca tgcttcgggc aaggtgcgcc ttgatgatat cgaccatgag ggaatctt
//
LOCUS       DAOSFP010000371         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_47102_length_2262_cov_3.881740, whole genome shotgun sequence.
ACCESSION   DAOSFP010000371 DAOSFP010000000
VERSION     DAOSFP010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47102_length_2262_cov_3.881740"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLI16_09555"
     CDS             complement(<1..381)
                     /locus_tag="PLI16_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOH84844.1"
                     /translation="MENKKVILVPTDFSEVCDNAVTYGAKLAKILGFGLTVLHIIDKK
                     SKAELKKNKQSLTEVDDRLEKIASATAKNFGVEVSTLSLAGDIFTDIGKTAAKIKATL
                     VILGTHGKVGLKQKFGMSFAKKVIA"
     gene            complement(417..1004)
                     /locus_tag="PLI16_09560"
     CDS             complement(417..1004)
                     /locus_tag="PLI16_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOH84845.1"
                     /translation="MNLDAATSYAIKELEPIEKTRYLYHNVYHALDVFKSVAAYGISV
                     NINADDLVLLKTAAVFHDIGIITSYEKHEEASVDVIKKVLPGFGYDDVQIEKISQLIM
                     ATKMPTNPKNELEKLICDADLDYLGREDYLEISERLRREWEVIRQIKYTEDDWLDYQL
                     SYLVTHKYYSQAAQTLRNAGKALNIIKMEELLKKT"
     gene            complement(1090..>2262)
                     /locus_tag="PLI16_09565"
     CDS             complement(1090..>2262)
                     /locus_tag="PLI16_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH84846.1"
                     /translation="QYLDLDHYPEPDYDDFFSVAQEDKKNRLFFSTPIESIRGCPWNR
                     CKFCVLNKGYRYRERAADSVGREVAAVLEKYRTAYFQFMDNSLIGKDKQRLNAMLDRL
                     TNISLTSRQDFSLSAEIIPHGLDAAFYKKLALAGFRMMQVGGESFADSLLRKMNKKNS
                     FSDNLLAYKFCLKYGIIPIGANIITGIPDETLRDIRTSISNIPFLRFFVGPQAITFTE
                     SKFALDKQSQYYKELDKKELESYTEHFLFNYLPPQMTSGMNRFELFYFCKNKSAAQGK
                     LWEKFFHTLSQYQKARFTYKIYNHEGVILYEEYCQGAKNDSIVFDQPVEWQILVNANT
                     QLCSFESMLKLLKNKYPGITAAELKNIMDELKSKHLIYFDDTYDKIISVIDTDNLL"
BASE COUNT          584 a          437 c          461 g          780 t
ORIGIN      
        1 ggcgataact tttttggcga agctcatacc gaatttttgt ttcagcccta cttttccgtg
       61 tgtgcccaaa attaccaggg tggcttttat tttggcagcc gttttgccga tatcggtaaa
      121 aatgtcgccg gccagggata aggtacttac ttcgacaccg aaattttttg ctgttgctga
      181 ggcgattttt tcaagccggt catccacttc ggtaagactt tgtttgtttt ttttcagttc
      241 tgctttggat tttttgtcaa taatatgcag caccgtgagg ccgaatccta atattttagc
      301 taatttggct ccataggtta ctgcattatc gcatacttca gaaaaatctg ttggtaccag
      361 aatcactttt ttattttcca tgttattatt ttttcagtta tgatttaaaa gcaaatttat
      421 gtttttttta ataattcttc cattttaatg atatttaatg cttttccggc attgcggagg
      481 gtctgtgccg cctgagagta atacttgtga gtaacaaggt agctaagttg ataatcaagc
      541 cagtcgtctt cagtgtattt tatttgtctt ataacttccc actcacgcct tagtcgttca
      601 gagatttcca ggtaatcctc gcgccccagg taatcgaggt cggcatcgca tattagtttt
      661 tcgagttcgt ttttcggatt ggtaggcatt tttgttgcca ttatcagttg gctgattttt
      721 tcgatttgca catcgtcgta tccaaaaccg ggtaacactt tttttatgac atctactgat
      781 gcttcttcgt gtttttcata ggaagttatg atgccgatat catgaaaaac agccgcagtt
      841 tttagcaaca ccaggtcgtc agcatttata tttactgata ttccataggc ggcaacagat
      901 ttaaatacat caagggcatg gtatacatta tgataaagat accttgtttt ttcaatgggt
      961 tcaagctctt tgatggcgta tgacgtggcg gcatcaagat tcatctgtca cgaaaattta
     1021 tacaatatta gtttttttat ctattgaaaa agaaacaatt tggttttttt tacaaatatt
     1081 ttcaagcagt tacagcaggt tatcggtatc tataacggaa attattttgt catacgtatc
     1141 atcaaaataa atcaggtgct ttgactttaa ttcatccatg atgtttttta actcagccgc
     1201 tgtaatccca gggtatttat tttttaataa cttcaacata gattcaaatg aacacagctg
     1261 agtatttgca ttgaccagta tctgccattc taccggctgg tcaaacacta tcgaatcatt
     1321 tttggcgccc tggcaatatt cttcatagag tattaccccc tcatgattat atatcttata
     1381 ggtaaaacga gctttttggt attgactgag cgtgtgaaaa aatttttccc agagtttccc
     1441 ttgagctgcc gatttatttt tacaaaaata aaataattca aaacggttca tcccgctggt
     1501 catctgcggc ggcaggtagt tgaaaagaaa atgttccgta tagctttcaa gttccttttt
     1561 atccagttct ttgtaatatt gtgattgttt gtcgagtgca aacttacttt ctgtaaatgt
     1621 tatcgcctga gggccgacaa agaatcttaa aaaagggatg ttgctgatac ttgtacgaat
     1681 atcccggagt gtttcgtcag gaatgccggt aatgatgttg gccccgatag gaataatgcc
     1741 gtatttaaga cagaacttat aagccagcag gttgtcggaa aaagaatttt ttttattcat
     1801 ttttctgagc agtgagtcgg caaaggattc tccgcccacc tgcatcattc ggaaccctgc
     1861 cagggctagt tttttgtaga aagcggcatc cagtccatgg ggtattattt ctgctgaaag
     1921 actgaagtcc tgccgggagg taagggatat attggtgagg cggtccagca tagcgttcag
     1981 acgctgctta tctttgccga taaggctatt gtccataaac tgaaaatagg ctgtccggta
     2041 tttttccagc acggcagcaa cttctctgcc cacgctgtct gccgctcgtt ctctgtaccg
     2101 gtagcccttg ttcaatacac aaaatttgca gcggttccac ggacagccac gtattgattc
     2161 tatcggggtg ctgaaaaaca gcctgttttt tttatcttct tgggctacag aaaaaaaatc
     2221 atcgtaatca ggctcggggt agtggtcaag gtcaagatat tg
//
LOCUS       DAOSFP010000372         2253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_47399_length_2253_cov_4.013194, whole genome shotgun sequence.
ACCESSION   DAOSFP010000372 DAOSFP010000000
VERSION     DAOSFP010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47399_length_2253_cov_4.013194"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLI16_09570"
     CDS             complement(<1..222)
                     /locus_tag="PLI16_09570"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOH84847.1"
                     /translation="MRNDVYVEWKYKEKGQDKVWRPHNVNGVFYNIPVSLRSAFTNSM
                     NGVAVTLAREVGIDKVIRYAYLLGIRTKLE"
     gene            complement(246..1727)
                     /locus_tag="PLI16_09575"
     CDS             complement(246..1727)
                     /locus_tag="PLI16_09575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOH84848.1"
                     /translation="MLKLILSKIKPYYQKTKQWFAARKKRHKQRRWHIRLLYDIMFVF
                     LLFILFLFLININFLWLFGKSPRISEIRNPQQSIASEVYTSDGKMIGRFFKENRTPLE
                     LHDLSPLLVKTLIAVEDQRFYSHNGIDFKGLFSAFRDWFSGNYRGASTISQQLVKNMY
                     KSRTAYSRGLFGHIPYVRIVIMKLKEWIGALKIEMFYSKEEILVMYLNTVDFGNNTFG
                     IKTASRIYFGVSPDKLKPQQVAMLIGMLKAPSTYNPLRNPKNCTKRRNVVLGIMKREN
                     LITEGQLDSLKALPLGLKYKVEDLSDGQATYFRMAVADAIENWCKENNLDIYTSGLKI
                     YTTIDSRMQQYAEDAVKKQMNILQSEFNRQWGTENPWRDTQYQEIPGFLQSNALESAR
                     YKALRKETGNADSAMALMRKPVAMTLYHPKKGLTDTVLSPLDSINYTLRFLHTGFVAI
                     EPQNRYVRAWVGDIDYRFFKFDHVSQSARQPGSTFKLFVYPPP"
     gene            complement(1800..>2253)
                     /locus_tag="PLI16_09580"
     CDS             complement(1800..>2253)
                     /locus_tag="PLI16_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOH84849.1"
                     /translation="NNIKFSDCEFIVCNLSLARLNNTALREIKFRDCKMLGLLFENCN
                     KFGLSVSFTNCILNHSSFYQVKIKGTKFIHSQIQEVDFTECDLSGAVFDNCNLDRAIF
                     QHTVLEKTDFSTAYGYAINPEINRLKKARFSSSGLTGLLYKYDLDIID"
BASE COUNT          606 a          479 c          494 g          674 t
ORIGIN      
        1 ctccagtttt gttctgatac cgagcagata ggcataccgt atcactttgt caatacctac
       61 ttcgcgggca agggttacgg cgacaccatt catggaattg gtaaaagctg aacgcaggct
      121 caccgggata ttgtaaaaca ctccgttgac attatgaggc cgccatactt tgtcctggcc
      181 tttttctttg tatttccatt ccacataaac gtcgttgcgc atgtcgcagg ggctaattcc
      241 cgatgctatg gcggcggata aacaaacaac ttaaatgtgg atcccggctg ccgtgccgat
      301 tgagatacat ggtcgaattt aaaaaaccgg taatctatgt cgcccaccca ggcgcgtaca
      361 taacggttct gcggctcgat agccacaaat cccgtatgca gaaaccgcaa tgtataattg
      421 atagaatcca atgggctgag caccgtgtct gtcagacctt ttttcggatg atataaggtc
      481 atggctaccg gcttacgcat cagcgccatt gcagagtcgg cattgccggt ttcttttcgt
      541 aaggctttgt agcgtgcgct ttccagggcg ttgctctgca aaaatccggg tatttcctga
      601 tattgtgtgt cacgccaggg gttttccgtt ccccattgcc tgttaaactc gctttgcaga
      661 atattcattt gtttttttac tgcatcttcg gcgtattgct gcatacgcga atctatggtg
      721 gtgtatattt tcaggcccga agtataaata tccaggttgt tttctttaca ccagttttca
      781 atggcatcgg ccacagccat cctgaaatag gtggcctggc cgtcagagag gtcttccacc
      841 ttgtatttca gaccaagggg cagggctttc agtgagtcaa gctggccttc ggtaatgagg
      901 ttttctcttt tcatgatgcc gagtaccaca ttccggcgtt tggtacagtt ttttggattg
      961 cggagcgggt tgtaggtaga gggggctttt aacatgccga taagcatggc cacctgctgt
     1021 ggctttaatt tatcgggtga tacgccaaaa taaattctcg aagctgtttt aattccaaag
     1081 gtattgttcc caaaatcaac ggtattgagg tacatgacca atatctcttc tttggaatag
     1141 aacatctcga ttttcaaggc gccaatccat tccttaagtt tcatgattac aatccgaaca
     1201 taaggaatat gcccgaatag tccccgagaa taggccgtac gcgatttata catatttttt
     1261 accaattgtt gggagatggt gctggccccc cggtaatttc ccgaaaacca gtcgcggaac
     1321 gccgaaaaca agcctttgaa atcaatacca ttgtgagaat aaaaacgttg gtcttctacg
     1381 gcaatcagcg ttttcacaag caggggcgac aagtcatgca attccagcgg ggtacggttc
     1441 tccttaaaaa aacggcctat catttttcca tctgaagtat aaacttctga agcaatgctt
     1501 tgctgaggat ttctgatttc ggatattcgc ggcgatttac cgaacaacca tagaaaattg
     1561 atgttaataa ggaaaagaaa aagtatgaat aacagaaaga caaacatgat gtcatacaat
     1621 aacctgatgt gccaccggcg ttgtttgtgc cgcttttttc gggctgcaaa ccactgtttt
     1681 gttttctggt aatatggttt tatttttgat aaaatgagct ttagcatgga gaaaaatttt
     1741 tgcaaatgat tacaaaacga tggtagcctg tttttataat ataaaattca attagctttt
     1801 taatcaatga tatcgagatc gtatttgtat aaaagtcctg ttaagcctga acttgaaaac
     1861 ctggctttct tcaaacggtt tatctcaggg ttgatcgcat agccatatgc cgttgaaaaa
     1921 tcggttttct caagcacggt atgctgaaaa atggcccggt cgaggttaca attgtcaaaa
     1981 acagcgccgc tcaggtcaca ttctgtaaaa tcaacttcct gtatctgtga atgaataaat
     2041 tttgtgcctt tgatctttac ctgataaaag gaagaatgat tcagtatgca attagtaaaa
     2101 ctgaccgaca ggccaaattt attgcagttt tcaaaaagca gccccagcat tttacagtcg
     2161 cgaaatttaa tttcgcgtaa tgccgtattg tttagcctgg ccagactgag gttgcacaca
     2221 ataaattcgc aatcactgaa cttgatgttg ttc
//
LOCUS       DAOSFP010000373         2242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_47718_length_2242_cov_4.520348, whole genome shotgun sequence.
ACCESSION   DAOSFP010000373 DAOSFP010000000
VERSION     DAOSFP010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47718_length_2242_cov_4.520348"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLI16_09585"
     CDS             complement(<1..655)
                     /locus_tag="PLI16_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOH84850.1"
                     /translation="MLIKTYGSAILGIDAITITVEVNIDSGVQFFMVGLPDSAVKESQ
                     HRIVASFKNSGFNYPGKRIVINMAPADIKKEGSAYDLTIALGILAASDQLKSDELGKY
                     IIMGELSLDGSLQPIKGALPIAIEARKQGFKGFILPEENAREAAIVNNLEVIGVKSLR
                     QVVDFLNGDIGIEPIAINTRDEFFTRLNEYEFDFADVKGQENIKRALEIAAAGGHNVI
                     "
     gene            888..1901
                     /locus_tag="PLI16_09590"
     CDS             888..1901
                     /locus_tag="PLI16_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84851.1"
                     /translation="MRKISLIALLFAGLFVFGASSSAKAQTQLNNSGFETWKDVGIST
                     EEPAQWNSFKSASGDAFLLTFAQQQIKRSTLTRPGSTGNYSCLIWSRTILSVVANGNV
                     TTGQINMGSATATDPANYNISRTADTTYSEALNAHPDSLVFWTKFKPSNTAGSDSGRV
                     RALIHDNYDAKDPLDAASQPHVVGDATLNFPSTNNQWVRISIPFNYTGPATSPDYMLI
                     TFTTNKTPGGGSGGDSLYIDDLSLVYSGSSSIVNSNIDPSENFFVYTDDNDIIIDLSF
                     NQLRTSKIEMYNMSGQLVYKTQITAQSVQHFVGINQFKSGIYVITANTDDGRKFSQKV
                     FIK"
     gene            1959..>2242
                     /locus_tag="PLI16_09595"
     CDS             1959..>2242
                     /locus_tag="PLI16_09595"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOH84852.1"
                     /translation="MRFRKIKYCFLFLIFTAGLNFPAVVFSQKAIIKGVVTEDGEGNP
                     IPYASIGVKDQQKGTLSDMDGNYLLEVIPGDYTLMFSCVGYEKYTQKISA"
BASE COUNT          647 a          511 c          437 g          647 t
ORIGIN      
        1 aaatgacatt atggccaccg gctgctgcaa tctcgagagc gcgtttaatg ttctcctgtc
       61 ctttgacatc ggcaaaatcg aattcatact cattcagccg tgtaaaaaac tcgtcgcggg
      121 tgttaatggc tattggctct atcccgatat cgccattcaa aaaatccacc acctgccgca
      181 acgatttcac tcctatcacc tccaggttgt taacaatggc ggcttctctg gcattttctt
      241 caggcagaat aaaaccttta aaaccttgtt ttctggcttc tatggcgatg ggtaaagcgc
      301 cttttattgg ttgcaggctg ccatcgagcg aaagttcacc cataataata tactttccta
      361 actcatcgga ttttaactga tcggaagccg ccagtatgcc aagagcgatg gtcaggtcgt
      421 aggcagagcc ttctttctta atatcggcag gagccatatt gatgacaatc cttttccccg
      481 ggtagttaaa accgctgttt ttgaacgatg caacgatgcg gtgctggctc tctttcaccg
      541 cactgtcggg caggccaacc ataaagaact gaaccccgga atcgatatta acctcaacgg
      601 taatcgtaat cgcatcaatt cccaggatgg cacttccata agtttttatt agcatggtgg
      661 tatttttaat ggcgataaca aatatatttc atttattatc atgctgtact gttttgttaa
      721 atattttatt ttttttacgc ttcacctaat caaaacacat aatttacaat gttttacgag
      781 cgtattaccg gggcgaacca tttaacaagt tcggcgcggt tgtgcgtatt tgattttttt
      841 gtaattttgc ccccgatttt taacaaccat aataaatcaa tacaactatg agaaaaataa
      901 gtttaatagc attattattt gcaggattat ttgtttttgg cgcatcatca tcggccaaag
      961 cacaaactca acttaacaat tccggttttg agacctggaa agatgttggc atcagtaccg
     1021 aagagccggc gcaatggaat tcttttaaat cagcctctgg agatgctttc ttactgactt
     1081 ttgcgcagca acagataaag cgttcgacgt taaccaggcc agggtccacc ggaaactata
     1141 gttgcctcat ctggtcaaga accatccttt cggttgttgc caacggcaat gtaaccacag
     1201 gtcagatcaa tatgggtagt gcaacggcca cagatccggc caattacaat atttcccgta
     1261 ctgcagatac cacttatagc gaagctctta atgcccatcc tgattccctg gttttctgga
     1321 caaaatttaa accctccaac accgctggat cagactctgg ccgcgtaagg gcacttatcc
     1381 atgacaacta tgacgcgaaa gaccccctgg atgctgcttc tcagccacat gtggttggtg
     1441 atgccacgct taattttcct tcaactaaca accaatgggt acgcattagc attcctttca
     1501 actacactgg tccagcaaca tctcccgatt acatgctgat tacttttacc acaaataaaa
     1561 cccctggtgg cggctcagga ggagacagcc tgtatataga tgacctctca cttgtttaca
     1621 gcggttcctc ttcaatagta aattcaaaca ttgatccctc ggaaaatttc tttgtatata
     1681 ccgacgacaa cgacatcatc atcgatttat cttttaatca actccgtact tcaaaaatag
     1741 aaatgtataa catgagcggg caattagttt acaagacaca aatcacggct cagtccgtac
     1801 aacattttgt cgggataaat cagtttaaaa gcggcatcta tgttataacg gccaatacgg
     1861 atgatggacg aaagttcagc caaaaagtat tcataaaata aaaaatgcaa cggttgggtt
     1921 gggtatttgc aggcgaaaga aaaactaaat ttttacccat gagatttcgc aagataaaat
     1981 attgttttct atttttaatt ttcacagcag gattgaattt tcctgctgtg gttttttcac
     2041 aaaaagccat tatcaagggt gtagtaaccg aggatggcga gggcaacccc attccgtacg
     2101 ccagcattgg agttaaagac cagcaaaaag gcaccttaag tgatatggat ggcaattatc
     2161 tgctggaagt tatacccggc gactatacgc tgatgttttc ctgtgttggc tacgaaaaat
     2221 acacccaaaa aatcagtgcc aa
//
LOCUS       DAOSFP010000374         2240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_47785_length_2240_cov_3.232494, whole genome shotgun sequence.
ACCESSION   DAOSFP010000374 DAOSFP010000000
VERSION     DAOSFP010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47785_length_2240_cov_3.232494"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLI16_09600"
     CDS             <1..238
                     /locus_tag="PLI16_09600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOH84853.1"
                     /translation="MAPDNVFVYVVAPGFVETEMSKNALEGKRGAEIKKQSPLNRVAK
                     PEEVANTIVLLASEHTEFLTGGIVDINGASYLRS"
     gene            complement(235..2010)
                     /locus_tag="PLI16_09605"
     CDS             complement(235..2010)
                     /locus_tag="PLI16_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84854.1"
                     /translation="MSFVLIMPKAFLRYGFLLLLLSALSGNRALQAQKNYTLTIVSSD
                     SSAKVLKTSHTAKSFNTKEDAADAVKELIASLFMQGFLEANIDSMSSDSARLKAYVTL
                     GKKYSYRTLRQGNLDKNLRNEVNFKEQYFAGKPFRYETVIKISDKLLEYAENNGYPFA
                     EFRLDSITVGDGTIDASINFQRNNKVLIDSVIVKGSAKMAKGYLYSYLSVKPGNLYNE
                     SLIRKVDQKLKDLPFVNVSKPMEILFTEDKARIYVYPDKKKASTFYGILGVLPSSQTT
                     GKLMINGELKLSLLNSFGRGELIDLNWRSITKGTQDLKVNLAYPYLFSTPFGINYKFM
                     LYKQDTSYLTLTHTIGLQYYFSGNNYIKVFADIYKSDLLNVAGLETATVLPDYADVSA
                     NLFGLEFTRETYDYRANPRKGYLAHFSFAGGQKKIRENSSVNSALYDSLKLKSSQYRF
                     IVNGRLFVPLFRKMTLLFATENGYLINSSIFENELFKLGGLNSLRGFDEESLRASYFN
                     ILAAEYRFLFERNSFVALFINAAYYEKNTRNAFVHDIPYGFGAGVSFDTKIGMFSLYY
                     ALGSQFGQAIAFKQSKIHFGYNASF"
     gene            complement(2022..>2240)
                     /locus_tag="PLI16_09610"
     CDS             complement(2022..>2240)
                     /locus_tag="PLI16_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOH84855.1"
                     /translation="LSNQKQGLVFLLWGKFAQGKEALIDISKHHILKAAHPSPLSAYN
                     GFFGCRHFSKTNELLRMQGKKEIDWAIE"
BASE COUNT          643 a          493 c          488 g          616 t
ORIGIN      
        1 gatggcgccg gataatgtgt ttgtgtacgt ggtggcgccg ggtttcgttg aaaccgaaat
       61 gtcgaaaaat gccctggaag gcaaacgtgg cgcagaaata aaaaaacaaa gcccgctgaa
      121 ccgcgtagca aagcccgaag aagtagccaa cactatagtt ttgcttgcct ccgagcatac
      181 cgagtttctc acaggcggca ttgtggatat caacggagcc tcatacctca gaagttaaaa
      241 actggcgttg tacccaaagt gaatttttga ctgcttgaag gcaatggctt gcccaaattg
      301 gcttccaagc gcataataca gtgaaaacat accgattttg gtatcaaaag aaacaccggc
      361 gccaaagcca tagggaatat catgaacaaa agcattgcgg gtatttttct cataataggc
      421 cgcattgata aaaagcgcga caaatgaatt tcgttcgaac aaaaaccggt attctgctgc
      481 aaggatatta aaataagatg cacgcaatga ttcctcgtcg aagccgcgta acgaattgag
      541 gccgccgagt ttaaacaact cgttttcaaa aatggaactg ttgatcaggt atccgttttc
      601 ggtggcaaac aacaaagtca tctttcggaa taaaggcaca aacaggcggc cattgacaat
      661 gaaccggtat tgggatgatt ttagtttaag gctgtcgtat aaggcgctgt ttaccgatga
      721 gttctccctg atttttttct gacctccggc aaacgaaaag tgagccaggt acccttttcg
      781 cgggttggca cggtaatcat aggtttcccg tgtaaactcc aggccgaaaa ggttggccga
      841 aacatcagca taatcaggca gcacagtggc tgtctccagt cctgccacat tcagcaggtc
      901 cgatttataa atatcggcaa agaccttaat ataattgttg cctgaaaaat aatactgcag
      961 gccgatagta tgtgttaatg tcaggtagct tgtatcctgc ttatagagca taaacttata
     1021 attgatgccg aagggagttg aaaaaaggta aggataagcc aggttgactt tcagatcctg
     1081 cgtacctttt gtaatactgc gccagttaag gtcgataagc tctccccttc caaatgaatt
     1141 taacagcgac agtttcagct caccgtttat catcagcttg ccggtggtct ggctgctggg
     1201 caacaccccc agaatgccat aaaaggtgct ggcttttttc ttgtcgggat agacataaat
     1261 cctggcttta tcctcggtaa acaaaatttc cataggcttg ctcacattca caaaaggcag
     1321 atctttcaac ttctggtcca ccttgcggat gagcgattca ttgtatagat tgcccggttt
     1381 aaccgaaagg taactataca gataaccttt ggccattttc gcgctgcctt ttacaatcac
     1441 actgtctata agcactttat tgtttcgctg aaaattgata ctggcatcga tagtaccatc
     1501 gccgactgtt atgctgtcta atctgaattc tgcaaaagga tagccgttat tttcggcata
     1561 ttccagcaat ttatccgaga tttttatcac cgtttcatac cggaaaggtt ttcctgcaaa
     1621 atactgttcc ttaaaattga cctcatttcg caggttttta tccagatttc cctgacgcag
     1681 tgtgcgatat gaatattttt ttcccagggt gacataagcc ttaagcctgg ccgagtcaga
     1741 cgacatactg tcgatgtttg cctccagaaa gccctgcata aaaagagatg caatcagttc
     1801 tttaaccgca tcggctgcat cttctttcgt attaaatgac ttcgcggtat gtgatgtctt
     1861 cagcactttt gccgagctgt cggaagaaac aattgtcagg gtatagttct tctgagcttg
     1921 caacgcacgg tttccgctta aagcagataa tagcaacagc aagaaaccat atcgcaaaaa
     1981 ggctttcggc atgataagta caaaactcaa atttacaaaa attattctat agcccagtca
     2041 atctctttct tgccttgcat acgtagcaac tcgttagttt ttgaaaaatg ccggcatcca
     2101 aagaagccat tataagccga caaaggcgat ggatgggcgg ctttcaatat atggtgtttg
     2161 ctgatatcaa tgagagcctc tttgccctgg gcgaactttc cccataaaag aaaaaccagt
     2221 ccctgttttt ggttggagag
//
LOCUS       DAOSFP010000375         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_48239_length_2226_cov_3.859972, whole genome shotgun sequence.
ACCESSION   DAOSFP010000375 DAOSFP010000000
VERSION     DAOSFP010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48239_length_2226_cov_3.859972"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="PLI16_09615"
     CDS             complement(<1..1684)
                     /locus_tag="PLI16_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84856.1"
                     /translation="MKLTLAEHIAQSRIIVAANKAKGNRHAEMFATMYAQPARFIEEI
                     LQNTEDAYARKKTKNFNNLVRFKLFGDRVEIHHNGKDFDEADLMSITTFAHTTKTNDS
                     SINQIGKFGIGFKSVFSITDTPEIHCEPYHYAITDYEVLENTEPKKPDENFNTLIILP
                     FKKKSQAICFDAVKNGLTELNEYSLLFLKKTSCIEIYFSVANALRIERRQTITDKIFR
                     LVSICKTYAQGNQETTDYIIFSQEPLAEKQQPELAFKTEKNQEKITFTPIDNAPVCVY
                     FPTRMNSRLHFILNAPFTTNPLREYIPFDSRLAPQNLRVLDESKKLFLKVLRSLKQKK
                     WFDLRLIAMLPLMTPDNNEPGPTTDSLIYRTFYDTLLPFLQSEAAIPLPGNRYAAIHD
                     VMMPHNGEIAALLDAKDLQVLFQKKYFVDPQINAKQFGEVKKYFEEVLQVKTADAQGF
                     GFRLRVAVDFLKEKKIPWLKKFYQYVHKQQFLWDEFHVSEYYSLRSAAIVLTQSGSFV
                     AAYTDGNRHGVFLPVGTKCLLPVIHKKLAGDTDCLAFFKDMGISEPEMADDVE"
     gene            1802..>2226
                     /locus_tag="PLI16_09620"
     CDS             1802..>2226
                     /locus_tag="PLI16_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOH84857.1"
                     /translation="MSEQKEKRTELTELGEFGLIDRLSAGIKLTNKSSVKGIGDDAAV
                     INNGDFYTLVSTDLLVEGVHFDLVYTPLKHLGYKSAVVNFSDICAMNGIPQQLTVGLA
                     VSSRFSVEAIEELYAGIKLACEKYNVDLVGGDTTSSTSG"
BASE COUNT          616 a          450 c          503 g          657 t
ORIGIN      
        1 actctacatc atcggccatt tcgggttcgc ttatacccat atctttaaaa aaagccaggc
       61 agtccgtatc accagcaagc tttttatgaa ttaccggcag taaacatttg gtgcccacag
      121 gtaaaaacac accgtgcctg ttgccatctg tataagcggc aacaaacgag cctgactgcg
      181 tcaaaactat agccgctgaa cgcaaactgt aatattccga cacatgaaac tcatcccata
      241 aaaactgctg cttgtgcacg tattgataaa attttttaag ccagggaatt tttttttctt
      301 tcagaaaatc aaccgctacc cgcaacctga aaccaaaacc ttgcgcatcc gcggttttaa
      361 cctgcagtac ctcttcaaaa tattttttta cttcaccaaa ctgttttgca ttaatttgcg
      421 gatcaacaaa atactttttc tgaaatagta cctgcaggtc cttagcgtcc aataaggctg
      481 caatttcccc gttgtgaggc atcattacgt catgaatggc ggcatatcgg tttccaggca
      541 gggggatggc ggcttccgat tgtaagaagg gcagcaacgt gtcataaaaa gtccgataaa
      601 tcaatgaatc tgtcgtgggg ccgggttcgt tgttatcagg agtcatcaac ggcaacatgg
      661 caatcaggcg gaggtcaaac catttttttt gttttaaact gcggagaact tttaaaaaca
      721 gttttttgct ttcgtcgagc acccgcaggt tttggggcgc caaacgggag tcaaacggaa
      781 tatattcccg caaagggttt gtggtgaaag gggcgttcag aataaaatgc aacctgctgt
      841 tcatccgagt gggaaagtaa acacaaaccg gcgcgttgtc aataggcgtg aaagttattt
      901 tttcctggtt tttttctgtt ttaaaggcga gttcgggctg ctgtttttca gcaagcggct
      961 cttgcgaaaa gataatataa tctgtggttt cctggttgcc ctgcgcatag gttttacaaa
     1021 tgcttaccag ccggaatatc ttatcggtta ttgtctgccg gcgttcgata cggagcgcat
     1081 tggcaacgga aaaataaatt tcgatgcagc ttgttttttt taaaaaaagc agtgagtatt
     1141 cgttcagttc cgtcaaacca ttttttaccg catcaaaaca tatagcttgt gattttttct
     1201 taaagggcag aattatcagt gtgttgaaat tttcatcggg ttttttcggt tccgtgtttt
     1261 ccagcacttc ataatcggtg atggcgtaat gataaggctc gcaatgaatt tcaggcgtgt
     1321 cggtgataga gaaaaccgat ttgaaaccga taccgaattt tcctatctgg ttgatgctgc
     1381 tgtcgttagt tttggtagta tgggcaaagg tggttatcga catcaggtcg gcttcgtcaa
     1441 agtctttgcc attgtggtgt atttcaacac ggtcgccgaa cagcttaaac cgaacaaggt
     1501 tgttgaaatt tttggttttt tttcgtgcat aggcatcttc agtattttga agaatttctt
     1561 cgataaaacg tgcaggctgt gcatacatcg tagcaaacat ctcggcatga cggttgcctt
     1621 tggccttgtt ggcagctact ataatacggc tttgggcgat atgttcggct aaagtgagtt
     1681 tcaaacgctg ttttttgcaa atttaaagta ataaaacaag attttatctc ataaaacaag
     1741 atttgatcat aaaaatcgaa aaatcaaaaa cgctgtcgta acaaaatagt aattttgcct
     1801 tatgagtgaa caaaaagaaa aacgtaccga attgactgaa ctcggtgagt tcggtctcat
     1861 tgaccgattg tcagcaggca taaaacttac caataagtca tcagtaaaag gcattggaga
     1921 tgatgccgca gtgataaaca acggcgactt ttatacgctg gtatccaccg acctgctggt
     1981 cgaaggggtg cattttgacc ttgtgtacac tccgctgaaa catttgggtt ataaaagtgc
     2041 cgtggtaaat ttttcagata tctgtgccat gaacggaatt ccgcagcagc tcacggtggg
     2101 cctggctgtt tccagccgtt tttcggtgga agcgatagaa gaattatatg cgggcattaa
     2161 actggcctgc gaaaaataca acgtagatct tgtgggcggc gataccacaa gcagtacttc
     2221 cggtat
//
LOCUS       DAOSFP010000376         2221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_48401_length_2221_cov_4.414589, whole genome shotgun sequence.
ACCESSION   DAOSFP010000376 DAOSFP010000000
VERSION     DAOSFP010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48401_length_2221_cov_4.414589"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1070)
                     /locus_tag="PLI16_09625"
     CDS             complement(36..1070)
                     /locus_tag="PLI16_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOH84858.1"
                     /translation="MKTTLNKRFSLILSLFFLCGVLAAQPINKTDSLGRKQGLWKKFS
                     GDTLKYQGTFLNDKPDGEFKYFYPDGKIKALTMYADSGRKAQTVMYFSNGVKNAEGLF
                     VDKKKDGLWIYYGANGKKISEENYKTGVKDGTWKYFYDNGKINKTENYKNNMLEGECL
                     EFYADSVVKTKCLYLQNKLNGKFQFFYNSGKILYTGIYKNDRKTGEWMFFSENGLGNR
                     KLTYNNGTLMKEEVLVPVKSGAGSWVVVGDIAFCFADKDGCHVKKNSGEEILSSTTLN
                     DFEKLLDPFNFFRVSPDFLISRWSLKDRKLFSKENPVIVLNPDPGRPVKVHPDKLEGF
                     MSWASLMKYE"
     gene            complement(1051..1620)
                     /locus_tag="PLI16_09630"
     CDS             complement(1051..1620)
                     /locus_tag="PLI16_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOH84859.1"
                     /translation="MFPHIANPHSWTLFLDRDGVINKKLPDDYVKSWDEFEFLPFATA
                     SLARLRKYFGRIIVVSNQQGVGKGLMSQADLEAVHEKMCRMITADGGFINKIYCCTDL
                     ASSGSLNRKPDIGMALQAQQDFPEIDFSKSLMVGDALSDMEFAAHLGMVAVYIGSVKI
                     IAENNKLKIDYVFNNLKELADFIYENNPE"
     gene            complement(1753..>2221)
                     /locus_tag="PLI16_09635"
     CDS             complement(1753..>2221)
                     /locus_tag="PLI16_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOH84860.1"
                     /translation="KAGGCIYFCGNGGSAADAQHIAAELSGRFMLDRDPLDAEALHVN
                     SSYLTSVANDYSFSEVYARLLRAKGRPGDMLIAISTSGNSPNIVRAVEAAKEAGLITA
                     GLTGASGGQMKNLVDILINVPSDETPRIQEAHILIGHIFCQQVEEEMFGKDRK"
BASE COUNT          552 a          536 c          466 g          667 t
ORIGIN      
        1 tcatggccaa cagcaacctt tctttttgcg gtttcttatt catacttcat tagcgaggcc
       61 cacgacataa aaccctcgag cttgtcgggg tgcactttta caggcctgcc cgggtcagga
      121 ttaaggacta ttacaggatt ttctttggaa aacaatttcc tgtcttttaa cgaccaccgt
      181 gaaataagaa aatcaggact gactcggaaa aagttaaacg ggtcgagcaa tttttcaaaa
      241 tcgttgagtg tggttgaact caggatttcc tcgccagaat ttttcttcac atgacatcca
      301 tctttatcag caaaacaaaa tgcaatatct cccaccacca cccaactgcc ggcaccgctt
      361 tttacgggga ccagcacttc ttctttcatc agtgttccgt tgttataggt aagttttctg
      421 ttaccaaggc cattttcact aaaaaacatc cactcgcctg tcttgcggtc gtttttgtaa
      481 attcctgtat aaagaatctt gcccgaatta tagaaaaact gaaacttgcc gttgagtttg
      541 ttttgcagat acaggcactt tgtctttaca acgctgtcgg cataaaattc aaggcattcg
      601 ccctccagca tattgttttt gtagttttcg gttttattga ttttcccgtt gtcgtaaaag
      661 tatttccatg ttccatcttt gacaccggtt ttatagtttt cttccgaaat ctttttccca
      721 ttggcgccat agtaaatcca cagcccgtct ttctttttgt ccacgaacag gccttccgcg
      781 tttttcaccc cattgctgaa atacataact gtctgggctt ttctgccgct gtcggcgtac
      841 atagtcaggg ctttgatttt cccatcggga taaaaatatt tgaactctcc gtcgggctta
      901 tcgttgagga aagtgccttg gtactttaga gtatccccgg aaaatttttt ccataggccc
      961 tgcttgcgtc ccagcgaatc ggttttattt atgggctgcg cagcaagtac tccacacaaa
     1021 aaaaataatg acagtataag tgaaaatctt ttattcaggg ttgttttcat aaataaaatc
     1081 agctaattct ttcagattat taaaaacgta atcgatctta agtttattat tttccgcgat
     1141 gattttgacg gagccgatat aaacagccac catacccagg tgggcagcaa attccatatc
     1201 gctcagggcg tcgcccacca tcagggattt ggaaaaatca atttcaggaa agtcttgttg
     1261 tgcctgcaaa gccatcccta tgtcgggttt gcggttgagg ctgcccgagg aagccaggtc
     1321 ggtgcagcaa taaattttat tgataaagcc gccatcagcc gttatcatgc ggcacatttt
     1381 ctcatgaaca gcctccagat cagcctggct catgaggcct ttgcccacac cctgctggtt
     1441 ggaaacaacg atgatcctgc cgaaatactt ccgtagccgg gccagcgaag ccgtggcaaa
     1501 aggaagaaat tcaaattcat cccacgattt tacgtaatca tcaggaagtt ttttgtttat
     1561 cacgccatcg cggtcgagaa acaaggtcca ggaatgaggg ttggcaatat gaggaaacat
     1621 caacagacta ttttaaattt cttatattta cgctcttcac gggcctccca atccatatta
     1681 tcagggtttt caggtattat catatccttt tttctattgc ttgcctgggt ctttaagagg
     1741 ttactcatcg gcttatttgc ggtcttttcc aaacatctct tcttccacct gctggcaaaa
     1801 aatatgccct atcaggatat gggcttcctg tatgcgcggg gtttcgtccg aaggcacgtt
     1861 gataagaata tccaccaggt ttttcatttg ccctccgctg gcgcctgtaa gtcctgctgt
     1921 tatcaggcca gcctctttgg cggcctctac cgctctgacg atattcggtg aatttcccga
     1981 tgtagagatg gctatcagca tatcgccggg ccttcctttg gccctgagca gtcgggcata
     2041 cacctcgctg aacgaataat cgttggctac cgaggtgagg tatgaagaat ttacatgaag
     2101 ggcttcggca tccagcgggt cacggtcgag cataaaacgg ccagacagct cggcggcaat
     2161 atgttgggca tcggcagcac tgcctccgtt accgcagaaa taaatacatc ctccggcttt
     2221 t
//
LOCUS       DAOSFP010000377         2214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_48638_length_2214_cov_3.509495, whole genome shotgun sequence.
ACCESSION   DAOSFP010000377 DAOSFP010000000
VERSION     DAOSFP010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48638_length_2214_cov_3.509495"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLI16_09640"
     CDS             <1..813
                     /locus_tag="PLI16_09640"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOH84861.1"
                     /translation="KKQHPKPHRLKCVLGLEAKNPGIVLHDADIEETVKECVTGALSF
                     NGQRCTALKIIFVHRKIAKPFCDRLSEEVEKLSVGMPWDEGVHITPLPEANKSEYFAE
                     LINDAITHGAKVINPNGGIYNKSCFFPAVLYDVNDKMRIYREEQFGPVVPVICYDDIE
                     EPINYVINSNYGQQASIFGNDADQIAKLVDTLVNQVSRINLNSQCQRGPDTFPFTGRK
                     DSAEGTLSVSDALRVFSIRALVAAKENDLNKTIIREIIKDDKSAFLTTDFIL"
     gene            898..1827
                     /locus_tag="PLI16_09645"
     CDS             898..1827
                     /locus_tag="PLI16_09645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOH84862.1"
                     /translation="MINEANNVAGKTPDRSLIAYLSILVILCTGFIVGAKAMGEKGLY
                     LAQAYMLTPALAAVLTRLFFYRNKFSDARLRLGKVKDYFRFWLFSLLIAAASFLVFYL
                     IGAIKWDFTGETFLKNLAEQFKNSGQDINSGLPEGVTPKTMMWLLMAGQLTVLNIVPG
                     LITGFGEEFGHRGFMYTRLKEHGFLLSIIVGGLIWFAWHLPLQLLIPAKDDFSTTQTI
                     LNYFILAIGSVCTFAYLVYVFEKSQSIWVVSLAHIVINNASAAFSYFIILENQLLANA
                     GLTITMLLVLFFGPGHYLFKIFKQQKPDISEQN"
     gene            complement(1844..>2214)
                     /locus_tag="PLI16_09650"
     CDS             complement(1844..>2214)
                     /locus_tag="PLI16_09650"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84863.1"
                     /translation="LYANTGKPDTAIQILNGIIGKKPDYSKAYNKLGEIYGRIYGNFS
                     KSVEYLDKAYALNPTDLETLRNLGTLYGLQGKFEPSLKYLLEAEKVKPDDKDILNKLS
                     ITYLNIGNRQKAEEYSLKSK"
BASE COUNT          600 a          420 c          522 g          672 t
ORIGIN      
        1 aaaaagcaac atccgaaacc gcaccgcctg aaatgtgtac tcggcctgga agcaaaaaat
       61 ccgggcatag tgctgcatga tgccgacatc gaagaaacgg taaaggaatg tgttacgggt
      121 gctttgtcgt tcaatggaca gcggtgtact gccttgaaaa ttatttttgt acaccggaaa
      181 attgcaaaac ctttttgtga ccggctgtct gaggaggtgg aaaaattatc tgtcgggatg
      241 ccctgggatg agggagtcca tattacgccg ctaccggaag caaataaatc ggaatacttt
      301 gcagagctta tcaatgacgc aataacgcac ggagcaaaag tgatcaatcc gaacggtggc
      361 atttacaata agtcttgttt ctttcctgcc gttttgtatg atgttaatga caaaatgagg
      421 atatacaggg aagagcagtt cggacctgtg gtgcctgtga tatgttatga tgacattgag
      481 gagccgatta attatgttat caattcaaat tatggacaac aggcaagtat tttcggtaac
      541 gacgctgacc agatagcaaa actggttgat acattggtga atcaggtgtc gcgtatcaac
      601 ctgaactccc aatgtcagcg aggccctgat actttcccgt ttaccggtcg caaagattct
      661 gctgaaggaa cattatccgt atcggatgca ttgcgtgttt tttcaatcag agctttggtt
      721 gctgcaaaag agaatgattt aaacaaaacc attatccgag aaataatcaa agacgataaa
      781 tctgcttttt tgactacgga ttttatttta taagtgttag tatgcctggc ggtaaggccc
      841 atgagtggtg tcgcaaaaga ttttgtcggt atgcctgatt gttaaataat aacagccatg
      901 ataaatgagg ctaataatgt tgccgggaaa acaccagata ggtcactgat tgcttatctc
      961 agcatactgg taatcctatg cacaggcttt attgttggag caaaagcaat gggggaaaag
     1021 ggcttgtatc tggcgcaggc ctatatgctt acaccggcat tggcggctgt gctgacaagg
     1081 ctattctttt acagaaacaa attttctgat gccaggttaa gactgggaaa ggtaaaagac
     1141 tatttcagat tctggctgtt ttccctgttg atagcggcag catctttcct ggtgttttat
     1201 ttgatcggtg ctataaaatg ggattttacg ggagagacgt tcctgaaaaa tctggcagaa
     1261 caatttaaaa attcggggca ggacataaat agcggactgc cggagggagt aaccccgaaa
     1321 actatgatgt ggctgctgat ggcagggcaa ctgaccgtat taaatatcgt ccccgggttg
     1381 attaccggat ttggggaaga gtttggacac aggggtttta tgtacacccg gttaaaagag
     1441 cacggatttt tgctaagcat tatcgttggg ggacttatat ggtttgcctg gcatctgcca
     1501 ttacagttgt taattcccgc gaaagatgat ttttctacga cccagacaat cctgaattat
     1561 tttattcttg ccatagggtc ggtatgtaca ttcgcctacc tggtttatgt ttttgaaaaa
     1621 tctcaaagta tctgggtggt gtctttggct catattgtta ttaacaatgc aagcgctgct
     1681 ttttcatatt ttatcatact tgagaatcaa ttgctggcca atgctggcct gacaatcacc
     1741 atgttgctgg tgctgttttt cgggccgggc cactatctct ttaaaatttt taaacaacaa
     1801 aaacctgata tttctgagca aaattgataa tgcaggagta aaactatttt gacttcaggg
     1861 aatattcttc tgctttttgc ctgttcccga tgttcagata cgtaatcgac aatttattca
     1921 ggatatcctt gtcgtcaggt tttacttttt cagcttccag caggtatttt aatgacggtt
     1981 cgaatttacc ctgaaggcca tacagcgtgc ccaggttcct caacgtttcc aggtcggtgg
     2041 gattcagcgc atatgctttg tccaggtatt caaccgattt gctgaaattg ccgtatattc
     2101 ttccgtaaat ttcacccagt ttgttgtatg ctttgctgta atcaggtttt ttaccgataa
     2161 tgccgttcag aatctgtatg gcagtgtcgg gtttgccggt attggcatat aaat
//
LOCUS       DAOSFP010000378         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_49060_length_2201_cov_5.051258, whole genome shotgun sequence.
ACCESSION   DAOSFP010000378 DAOSFP010000000
VERSION     DAOSFP010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49060_length_2201_cov_5.051258"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLI16_09655"
     CDS             complement(<1..536)
                     /locus_tag="PLI16_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOH84864.1"
                     /translation="MFFVDTHAHLYAEEFSEDIDEVIRKATQRGVTKLLLPDIDSSSR
                     NSMLALHLRYPETCLPMIGLHPTSVKEDYQAELQEIEKIITAQKFYGIGETGIDLYWD
                     KTFLREQIEAFTFQAELSLRYNLPLVIHSRKSLHEIFPILKKYCNRGLRGVFHCFPGN
                     ISEARQVIDMGFYLGIGGV"
     gene            complement(564..1283)
                     /locus_tag="PLI16_09660"
     CDS             complement(564..1283)
                     /locus_tag="PLI16_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOH84865.1"
                     /translation="MTVLSVIIPAYNEGKTIHLILDKVKAVQLISNIEKEVIIVNDCS
                     KDDTEEAIHAYQHANPDLNIQYFKHEKNAGKGAALHTGIEKATGDYLIIQDADLEYDP
                     EEYNILLRPIISGFADVVYGSRLIGGKPHRILFFWHSIGNRMLTAFSNMFTNLNLSDM
                     ETCYKLFRREFIQGIRLRENRFGFEPEVTAKIARIKNIRIYEVGISYYGRTYAEGKKI
                     SWKDGFRAIYCIMKYGLFKSK"
     gene            1394..>2201
                     /locus_tag="PLI16_09665"
     CDS             1394..>2201
                     /locus_tag="PLI16_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84866.1"
                     /translation="MYKNHRIFFSLGLAGIFALFFLFLAYYHEYKLFVPKPPVISMTI
                     PLAGQPAIPNTFLYDFENVSGLENLTTERSFSSQYALKVKGKRNATPPVQIQLDTVMA
                     QSGEAHYGAWICKAGTAEILKGLLVFQIVDQSNKVKYSALSPVEEKDGSQASWFYVCG
                     KADWKESNVQPGDLAKVYFWNNTSNTVYIDDVLAIFGKQSIKGEKNLSEKSFNKKPGI
                     TSVNQPPYPVFIFEKEFVGNVKNTTVFLTDGIDSLKFFPDDEYVPGKFIKS"
BASE COUNT          600 a          459 c          469 g          673 t
ORIGIN      
        1 acgccgccaa ttcccaggta gaaacccata tcgataacct gccgggcttc gctgatgttt
       61 ccgggaaaac aatgaaaaac accgcgtagc ccccggttgc aatatttttt taaaatggga
      121 aatatttcat gaagtgattt tcgggaatgt attaccaatg gtaagttgta gcgcagggaa
      181 agctctgcct gaaatgtgaa tgcctctatc tgttcgcgga ggaaagtttt atcccaatac
      241 aggtctatgc ccgtttcacc aataccgtaa aacttttggg ctgtaataat tttttctatt
      301 tcctgcagtt cggcctggta gtcttctttt accgaagtag gatgcaggcc tatcatcgga
      361 aggcatgttt cgggatatct gaggtgcaga gccaacatgc tgtttcggct actgctgtca
      421 atgtcgggca aaagaagttt ggttactccc cgttgtgtcg ccttgcggat aacctcatca
      481 atatcttcag aaaattcttc agcatacagg tgggcatggg tatcaacaaa aaacatgaga
      541 tgggaacttt accggcagtt tatttatttg cttttgaaaa ggccgtattt cattatacag
      601 tatatggccc tgaatccgtc tttccagctt attttttttc cttcggcata cgtacgcccg
      661 taataagata tgcccacctc ataaatcctg atgtttttta tccgggcaat tttggcggta
      721 acttccggtt caaaaccaaa acggttttcg cgcaggcgga tgccttgtat aaattcccgc
      781 cggaaaagtt tgtagcaggt ctccatatcc gacaggttga ggttggtaaa catattggaa
      841 aatgccgtga gcatgcggtt tcctattgag tgccagaaaa acaatatgcg gtgcggtttg
      901 ccgccaatca gccgcgaacc ataaaccaca tcggcaaagc cactgatgat cggacggagt
      961 aaaatgttgt attcttcggg atcgtattct aggtcggcat cctgaatgat cagatagtcg
     1021 cccgtggctt tttcaatgcc ggtatgcaag gcagcacctt ttcccgcatt tttttcgtgc
     1081 ttgaaatatt ggatgttcag gtccggattt gcatgttgat aagcatggat ggcttcctcc
     1141 gtatcgtctt ttgaacaatc attgacaata attacttctt tttcgatgtt gctgattagt
     1201 tgaaccgctt tgaccttatc gagaatcaga tgtattgttt taccttcatt ataggcaggg
     1261 atgattacag ataatactgt catactgtgt tattgttttt cacaaaaata tcaataataa
     1321 tagttcagta tcaattgtcg gtaaaataat tattacataa tttcgttcgt atctttgcct
     1381 aaaattaccg tggatgtata aaaaccaccg catatttttt tctcttggcc tggcaggtat
     1441 tttcgccttg ttttttttat tccttgccta ttaccatgaa tataagcttt ttgtgcctaa
     1501 gcctccggtg atctccatga ccataccctt ggccggccaa cctgcgattc ccaatacatt
     1561 tttatatgat tttgaaaatg tttccggcct ggagaacctc accacagaac gctctttcag
     1621 cagccaatac gcgttaaaag taaaaggaaa aagaaacgcc actcctccgg ttcaaataca
     1681 gctcgatacc gttatggcgc aaagcggtga agcgcattat ggcgcctgga tatgcaaggc
     1741 cggtaccgcg gaaatcttga agggcttact tgttttccaa attgttgatc agtcgaacaa
     1801 agtaaaatat tctgcgctat ctcctgtcga agaaaaagat ggttcacagg cttcgtggtt
     1861 ttatgtgtgc ggaaaagctg attggaaaga aagcaacgtg caacccggcg atctggccaa
     1921 agtttatttc tggaacaata ccagtaacac ggtatatatt gatgatgttc tggcgatctt
     1981 tggcaaacag tctattaaag gtgaaaaaaa cctgtcagag aagtcattca ataaaaagcc
     2041 gggcatcact tctgttaatc agccgccata ccctgttttt atttttgaaa aagaatttgt
     2101 tggtaacgta aagaatacga cagtgttttt aaccgacgga atagattcac tgaaattttt
     2161 tcccgatgat gaatatgtcc cgggcaaatt tataaaatca g
//
LOCUS       DAOSFP010000379         2180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_49749_length_2180_cov_4.382118, whole genome shotgun sequence.
ACCESSION   DAOSFP010000379 DAOSFP010000000
VERSION     DAOSFP010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49749_length_2180_cov_4.382118"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PLI16_09670"
     CDS             complement(<1..1116)
                     /locus_tag="PLI16_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84867.1"
                     /translation="MKKNLLSCFFFAFIVLSTWAQDVGIGEWQVYLPYRKAIAVDIVG
                     EKVYCATPNSIFYYDKTDNSVNTLTKNNGLSDVNISTIRYSPQFGYLIVAYTNANIDL
                     VADGEIINISDIKRKPIMGNKAINNITVYGDYAYLSCGFGIVVLDIARKEIKDTYYIG
                     PDGNAINVLDLACDGIKFYAGTEKGLYYADYANANLANYANWKKESRLPDTNATYNSV
                     AVFQGKVFANRVNPAYNQDTLYVLNDTIWQVLDTVYNDDVYNMRVLNNHFLIAYTSHV
                     AVYNANMIKEEIIWNYQPGYAYPNCAVYDSDSATWIADKNYGLVCSKNWTITKITPNG
                     PGNNNVFAMDIAGSDLYVAPGGVNNSWGNSWNNSGIFS"
     gene            complement(1167..>2180)
                     /locus_tag="PLI16_09675"
     CDS             complement(1167..>2180)
                     /locus_tag="PLI16_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOH84868.1"
                     /translation="ETVCVAKIDLKKRRLTQNNHTATHLLHKALREVVGSHVEQKGSY
                     VSDERLRFDFSHFTGVTAEEQRKIEKMIFEKISENLPVNAETDTMENARKKGAIALFG
                     EKYGNEVRMITVGDDYSRELCGGTHVQATGQIGLFIIVEETAVAAGVRRIEAITGETA
                     FDYIFQQYLEPLNQIKSAMKKPYAELPAMVNALIDSNTSLKKQIEGFQREKAQELKNT
                     LRAKVENIDGINFIAEQVDVDAASIKDIVFSLKNDIDKLFVVLGNVSEGKVAITLMIS
                     DDLVEANKWNASKIIRDFAKEIQGGGGGQDFYATAGGKNPAGLPNVFSKAREFVAINQ
                     RKQ"
BASE COUNT          537 a          510 c          471 g          662 t
ORIGIN      
        1 tgaaaatatg ccgctgttat tccaggagtt tccccaggag ttattcactc ctcccggagc
       61 tacatacaag tcgctgccgg caatgtccat agcaaacaca ttgttgtttc cgggtccgtt
      121 gggtgtgatt ttggtgattg tccagttttt gctgcatacc agcccatagt ttttgtcggc
      181 aatccaggtg gcgctatcgc tatcatacac ggcacaattg ggataggcat aaccgggttg
      241 ataattccag attatttctt ctttaatcat attggcattg tacaccgcca catgcgaagt
      301 ataagcaatg aggaaatggt tattgagaac gcgcatattg taaacatcat cgttataaac
      361 ggtatcaagc acctgccata tggtatcatt cagcacatat aaggtgtcct gattatatgc
      421 cgggttaaca cggttggcaa atacctttcc ctgaaaaaca gccacggagt tataggtggc
      481 attggtgtcg ggcaggcggc tttctttctt ccagttggca tagttggcga ggttggcatt
      541 ggcatagtcc gcataataaa gccctttttc ggttccggca tagaatttta ttccgtcgca
      601 ggcaagatcc agcacgttaa ttgcatttcc gtcggggccg atataatagg tatctttaat
      661 ttcctttctg gcaatatcca gcaccacaat gccaaagccg cacgacaggt aggcataatc
      721 cccataaacc gtgatattgt tgatggcttt gtttcccatg attggcttac gtttgatatc
      781 cgagatattg attatttcac cgtcagccac caggtcgata ttggcgttgg tgtatgccac
      841 tatcagataa ccaaattgcg ggctgtaacg tatagtactg atattgacat ccgataatcc
      901 gttgtttttg gtcagggtgt tgacgctatt atcggtttta tcataataaa aaatactgtt
      961 gggggtagcg caatacactt tttctccgac gatatcaaca gcgatagcct tccggtacgg
     1021 aaggtacacc tgccattcgc cgatgccgac atcctgtgcc catgttgaaa gaacaataaa
     1081 agcaaagaaa aaacagctga gcagattttt tttcatcaga aaaaatttgg tatgaaatta
     1141 ttaaaaaaaa ctgttttttc tcaaaattat tgtttgcgtt ggtttatagc aacaaattcc
     1201 cgggctttag agaaaacatt aggtagtccg gcaggatttt ttccgcccgc tgtggcataa
     1261 aaatcctgtc cgccaccacc gccctgaatt tctttggcaa agtcgcggat tatttttgac
     1321 gcattccatt tattggcttc caccaggtcg tcggaaatca tcagggtgat tgcaaccttg
     1381 ccttcgctga catttcccaa aaccacaaac agcttgtcga tatcattttt caatgaaaag
     1441 actatgtctt tgatgctggc agcatccaca tccacctgct ccgcaatgaa atttatcccg
     1501 tcaatatttt caacttttgc cctgagggta tttttcaatt cctgagcttt ttcacgctga
     1561 aagccttcta tctgtttttt cagggatgta ttgctgtcta tcaaggcgtt aaccatcgct
     1621 ggcaactcgg cataaggctt tttcatggcc gattttatct gattgagggg ttcgaggtac
     1681 tgctgaaaaa tataatcaaa agccgtttca cccgtaatgg cctctatacg gcgcacaccg
     1741 gctgccacag cggtttcttc cacaataata aacagcccta tttgtcctgt ggcctgcaca
     1801 tgagtacctc cgcacagttc acgggaatag tcatcgccca cggttatcat gcgcacttcg
     1861 ttgccatatt tctcgccaaa cagcgcgata gcgccctttt tacgggcgtt ttccatggta
     1921 tctgtttcgg catttaccgg gaggttttcc gatatttttt caaagatcat tttttcaatt
     1981 ttccgttgtt cttcagccgt tacaccggta aaatgcgaaa agtcgaagcg caggcgctca
     2041 tcactgacat acgacccctt ttgttccaca tggctgccga ccacttcgcg cagggcctta
     2101 tgcaggaggt gcgtggcggt gtggttgttc tgtgtcaacc ggcgcttttt caggtcgatt
     2161 tttgccacac aaaccgtttc
//
LOCUS       DAOSFP010000380         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_49849_length_2177_cov_3.705467, whole genome shotgun sequence.
ACCESSION   DAOSFP010000380 DAOSFP010000000
VERSION     DAOSFP010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49849_length_2177_cov_3.705467"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLI16_09680"
     CDS             complement(<1..985)
                     /locus_tag="PLI16_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84869.1"
                     /translation="MKTRIIFILFVFAWLGAGAQTQILFQSDQTKSYLLSFTNSYEES
                     QQVINEIVDAISKTIPKPVYQTKITLNITESVKVTRKGNVVSIYVSYQDMKINGDIFY
                     KGFNMAEVLFPSKYEFKATLSRKNGTVLYNFTQPKNDFKPDFNEVLLQCNDDSLSTAY
                     NFIINETKLYYDMVARDRFRQKAGMIDQYFTADMDLNNIGKQLSTINPNAFEETEKTQ
                     ASLNSFNTMIDNIGQAPFWKMLQIESFDPLMLKTKIVDIKKSLTAIQSQLNYTKSIIH
                     ELYYNKGTDLYEKNKMAEAKTAFEKSLQYYPNYSPSQYFMAQIAYETNQIDE"
     gene            1236..>2177
                     /locus_tag="PLI16_09685"
     CDS             1236..>2177
                     /locus_tag="PLI16_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOH84870.1"
                     /translation="MSGFFGCISKEDCVKDVFYGTDYHCHLGTKRAGMVFYSKEKGFQ
                     RAIHSLEDGYFRNKFEGNIAGFSGNSGIGVISDTESQPIIVSSHLGWFAVASVSKINN
                     IGELEQRFMKEHRLFTEISQGAINPTELIAMLIAEQEDFVSGIRNVFDAIQGSCSMLI
                     ITQDGIIAARDKLGRTPVILGKKSNAYAATFETIAFPNLGFEVEKYLGPGEIVRLTAE
                     GYEQLRKPDEKMQICSFLWVYYGYPPSYYEGINVDDCRYECGRALARHDTVEADFVAG
                     IPDSGIGHALGYSNQKQLPYKRPYIKYTPTWPRSFMPQ"
BASE COUNT          601 a          419 c          496 g          661 t
ORIGIN      
        1 cttcgtcaat ttggttggtt tcataagcta tctgcgccat aaaatactgc gaaggagagt
       61 agttgggata atactgtaat gatttttcaa aggcggtttt ggcttctgcc attttgtttt
      121 tttcatagag atccgtgccc ttgttgtaat acaattcatg aattatggat ttcgtataat
      181 tcaactgact ttggatagcg gtaaggcttt ttttgatgtc aacgattttg gttttcagca
      241 tcagcgggtc aaaactctct atctgcagca ttttccagaa aggtgcctgg ccaatgttgt
      301 ctatcatggt gttaaaactg ttcagcgagg cttgtgtctt ttctgtttct tcaaatgcgt
      361 tggggttgat ggttgagagt tgtttgccga tattgttcaa atccatatcg gcagtaaagt
      421 actgatctat catgccggct ttctgacgga agcggtcgcg ggcaaccata tcatagtaca
      481 acttggtttc attgatgata aagttgtagg cagtagataa cgaatcgtcg ttgcattgaa
      541 gcaatacttc attaaaatct ggtttgaaat cgtttttggg ctgggtaaaa ttatacagca
      601 ctgtgccgtt tttgcggctc agggtggctt taaactcata tttggaaggg aacaacactt
      661 ccgccatatt aaaacccttg taaaagatat ctccgttgat tttcatatcc tgataggaga
      721 cataaatgct caccacattg cctttacgcg ttaccttcac actttcagta atatttaagg
      781 tgatttttgt ctgatatacc ggcttaggga tggtttttga aatggcatcc actatttcgt
      841 tgataacctg ttggctttct tcgtatgaat tggtaaaact cagcaggtat gattttgtct
      901 gatccgactg gaacaatatc tgagtttgtg cacctgcgcc aagccatgca aatacaaata
      961 aaataaatat gatgcgggtt ttcataaagt gtaaaaatta gtgatacaaa aatataattt
     1021 tgtgctgata tataagtgca aaatctcaaa tgatgggcag aaaaacacct gcgaaaacca
     1081 aaatgacggg gtggaaaaag ttttattaca tccagaccgg tatccttctg ttatgatgat
     1141 tgctaacttt tcctgcctgt tcaaaatatg ctgataactt ttccggaaag aatgcgtggt
     1201 tatctataat tttaaatttt aataaaacaa aaactatgag tggatttttt ggttgcatct
     1261 ctaaagaaga ttgtgtaaaa gatgtctttt acggtactga ttatcattgt catctgggaa
     1321 caaaaagggc cggtatggta ttttattcta aggaaaaagg atttcagcgt gcaattcaca
     1381 gcctcgaaga cggttatttt cgcaacaaat tcgaaggcaa tattgccggt ttcagcggca
     1441 attcgggcat aggcgttatc agcgatacgg aatcgcaacc catcatcgtt tcttctcacc
     1501 tgggatggtt tgctgtagcc tcagtgagca aaatcaataa tataggggaa ctggaacagc
     1561 gttttatgaa agagcaccgc ctgtttaccg aaatcagcca gggcgccatc aaccccacag
     1621 agctcatagc catgctgatt gccgaacagg aagactttgt ttcaggaatc cggaatgttt
     1681 ttgatgctat tcagggatct tgctccatgc ttatcataac tcaggatggc attattgccg
     1741 ccagggacaa actggggcgc actccggtta ttttgggaaa aaagagtaat gcttacgctg
     1801 ctacatttga aaccattgcc tttcccaacc tgggtttcga ggtggaaaag tacctggggc
     1861 ccggcgaaat agtgagactg actgctgaag gttatgaaca actgcgtaaa ccggatgaaa
     1921 aaatgcagat atgttctttt ttgtgggtgt actatggcta tccgccatca tattatgagg
     1981 gcatcaatgt ggatgattgc cggtatgaat gcggcagggc gctggcccga cacgacacgg
     2041 tggaggccga ctttgtggcc ggcattcccg attccggcat cggccatgcg ctggggtaca
     2101 gcaaccagaa acaactgccg tacaaaagac cttatattaa atacacgccc acctggcccc
     2161 gaagttttat gccacag
//
LOCUS       DAOSFP010000381         2174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_49948_length_2174_cov_4.343558, whole genome shotgun sequence.
ACCESSION   DAOSFP010000381 DAOSFP010000000
VERSION     DAOSFP010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49948_length_2174_cov_4.343558"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..268
                     /locus_tag="PLI16_09690"
     tRNA            192..268
                     /locus_tag="PLI16_09690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:226..228,aa:Arg,seq:acg)
     gene            complement(505..810)
                     /locus_tag="PLI16_09695"
     CDS             complement(505..810)
                     /locus_tag="PLI16_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOH84871.1"
                     /translation="MAKINENTRKEVKETVFSFFTEECEVDAASLKENTNIIRDIGGD
                     SLMYLELIEIFKKKYSLNIELKTIGKYVVKHPVETIEQLIDMTMLIIEHENNIVNLD"
     gene            complement(834..2048)
                     /locus_tag="PLI16_09700"
     CDS             complement(834..2048)
                     /locus_tag="PLI16_09700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOH84872.1"
                     /translation="MEKVVVTGMSMISSLGTDLQSNWENLTAGKSGVKNITLFDASSC
                     QTRIAAEIPHTFEELWPKYIKKRSADQMTRITKACFVCAKDAVAMSGINFDLMDKDRC
                     GVILGVVTTANTSSETGTTDQNRILKSMNNAMSAWISLEYKLQGPNFTVSSACASSAY
                     AIALGYNMIKNGMADVVIVGGADSIVNKEEIDGFNSLYAISTENHTPEKASKPFSKDR
                     DGFVIGEGAGVLVLESERSALARQATIYAEMAGYALSSEAYNIMAPMKDGEGIAHCIE
                     QALKNAGVETSQVDYINAHGTSTTLNDKYETLAIKKVFGDMAYQIPISSSKSMIGHTI
                     GAAGAIELIITIQTIRHSIITPTINLDQPDPELDLDYVPNQARNRQVNCALSHSFAFG
                     GHNAVLVIKKYH"
BASE COUNT          554 a          463 c          463 g          694 t
ORIGIN      
        1 ccactgctgg gtatttcaaa caccctgata gctttgggat tgttcaatgc ctgtgccata
       61 ataatcatgg taataaataa aaagtttttt agccgactgg cataaaagga cttactcttg
      121 aggtttaaaa atgtttagga aaattttttt ggattttctt attaaaaagt attaattttg
      181 cacccgcaaa aggccccgta gctcaactga atagagtatc tgactacgga tcagaaggtt
      241 acaggtttga atcctgtcgg ggtcaccaga taaaaaagag ttcagcagta aacacggttg
      301 aactcttttt ttataagcag gaatgtaacc gaatgtggtg catttattct attgcaattg
      361 gcataaattt cctgtccgat ggttatttgg aagacatttt aaggggtgtc agaacgcgtc
      421 attactatag acatgctata tagtatcaga ccgctggcag aggtacctga actgcaattg
      481 acctttaacc gtagagataa actatcagtc taagtttaca atattgtttt cgtgctctat
      541 gatgagcata gtcatatcaa tgagttgttc aatagtttcc accggatgct tcactacata
      601 tttgccaatg gttttcaact ctatgttcag ggaatatttc tttttgaaaa tttctatcag
      661 ttcgaggtac atcaatgaat cgccgccaat gtcgcggata atattggtgt tttctttaag
      721 agaggcggca tcaacttcgc attcttcggt aaaaaaactg aatacggttt cttttacttc
      781 tttccttgtg ttttcattaa tctttgccat cactaaaatt ttttttgaca aaattaatga
      841 tattttttaa ttaccaaaac ggcattatgt ccaccaaaag caaatgaatg cgataatgcg
      901 cagtttactt gcctgttgcg ggcctggttg ggaacataat ccaggtcgag ttcgggatcg
      961 ggctggtcca ggtttatggt gggcgtgatg atgctgtgtc tgatagtttg tatggtaatg
     1021 atcaattcta tggctccggc ggcgccgatc gtatggccta tcatagactt cgaggaagat
     1081 atcggaattt ggtaggccat gtctccgaat acttttttta tagctagtgt ctcgtatttg
     1141 tcgtttaacg tggtggaagt tccatgagcg ttgatatagt caacctgcga agtttccact
     1201 cctgcgtttt tcagtgcctg ctcaatacaa tgagcgatgc cttcgccgtc tttcatgggg
     1261 gccatgatat tgtatgcctc gctggataag gcataacctg ccatctcggc atatatcgtt
     1321 gcctgccggg caagcgccga ccgttccgac tccaaaacca acacaccggc tccttcacca
     1381 atcacaaaac catcgcgatc tttcgaaaaa ggtttgctgg ctttttccgg tgtgtgattc
     1441 tctgtggaaa tagcataaag actgttaaat ccgtcaatct cctctttatt cacgatggaa
     1501 tcggctccgc ccacgatcac cacatctgcc atgccgtttt taatcatatt gtaaccaagg
     1561 gcaatggcgt aggctgagga ggcacacgcg gatgataccg tgaaatttgg gccctgcagt
     1621 ttgtattcca gagaaatcca tgccgacatt gcattattca tgcttttcaa aatgcggttt
     1681 tggtccgtgg tgcctgtttc agaggaggta ttggccgtgg taaccacccc caggatgaca
     1741 ccgcagcggt ctttgtccat cagatcgaaa tttataccgc tcatagcaac ggcatctttg
     1801 gcacaaacaa aacatgcttt ggtaatcctt gtcatctggt cagcagatct ttttttgata
     1861 tactttggcc aaagttcctc aaatgtgtga ggaatctccg ctgcaatgcg agtctgacag
     1921 gacgaggcgt caaacaaggt aatatttttc accccgcttt tgccggctgt aaggttttcc
     1981 caattgcttt gcaggtctgt tccaagcgag cttatcatac tcatccctgt tactacaact
     2041 ttttccataa ttttttcatt tattgttttt cagccaaggt caaatggccc tttatcagta
     2101 ttcatatact ctttggctgc atagtcaaga gtttccctgt cgcaggcccc gtcatctatc
     2161 agttgttgag cctt
//
LOCUS       DAOSFP010000382         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_50260_length_2165_cov_4.036019, whole genome shotgun sequence.
ACCESSION   DAOSFP010000382 DAOSFP010000000
VERSION     DAOSFP010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50260_length_2165_cov_4.036019"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLI16_09705"
     CDS             complement(<1..229)
                     /locus_tag="PLI16_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HOH84873.1"
                     /translation="MNQPLAFVHPQAKIAKNVVIEPFVTIDKNVEIGEGTWIGPNATI
                     MEGARIGRNCKIFPGAVISAIPQDMKYAGEDT"
     gene            complement(226..1632)
                     /locus_tag="PLI16_09710"
     CDS             complement(226..1632)
                     /locus_tag="PLI16_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOH84874.1"
                     /translation="MAKQRTVASPVSLTGKGLHTGNNVIITFHPAPENHGIVFKRIDL
                     ENQPMVHAVLGNVKDTSRGTSIEENGVGVHTIEHTMAALSGLSIDNVLIEINSCETPI
                     LDGSAIKYVELLKTAGTIEQDALKKVISLNTNVLYKDEKNKVEILAIPSDTFKITTLI
                     DYDSDIPGKQFAMLDDIVDFEKEIAPCRTFVFLHELEFLLKNNLIKGGDLDNAIIFVN
                     KIISQEELDRLATLFNKPKVSVLKEGILNNVELHFQNEPARHKLLDLIGDLSLLGGTL
                     NAHIIAKRPGHFSNIEFARKIAETVGVNSRSPHKASVSLPSLTKTPLYDNIQIQNILP
                     HRPPFLLLDKILEMDDKMVIGIKNVTINEGFFTGHFPGKPVMPGVLQIEAMAQCGGIF
                     ALNTVPDPENYLTYFLKIDNARFKKMVVPGDTLVFKLELLDPMRRGICHMQGSAYVNN
                     QLVMEAELLAQIVKITKS"
     gene            complement(1743..>2165)
                     /locus_tag="PLI16_09715"
     CDS             complement(1743..>2165)
                     /locus_tag="PLI16_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOH84875.1"
                     /translation="IGALNPVNGRTPCIITEDMVREMPFGSVIIDVSIDNGGCSETSH
                     ATNHTQPVFKKYGITHFCVPNIPSKVPHTASTALSNFFAPVILQIAEEGGIENFLRNE
                     KSVRMGTYMFSGILTSKYISDLFHIPFQEIDLLMLPFH"
BASE COUNT          534 a          418 c          533 g          680 t
ORIGIN      
        1 tggtgtcttc accggcatat ttcatgtctt gcggtatggc cgaaatgacg gcgcccggaa
       61 aaattttgca gtttctgcct atgcgtgcac cctccataat ggtagcattg gggcctatcc
      121 atgtgccttc gccgatttca acatttttgt caatggtaac aaaaggttct ataactacat
      181 ttttggcaat tttggcttgc ggatggacga atgctaaggg ttgattcatg atttggttat
      241 ttttactatt tgcgcgagga gttcagcttc catcaccagt tggttgttga cgtaggcgct
      301 gccttgcatg tgacagatac ctcttcgcat ggggtcgagg agttcaagtt taaagaccag
      361 ggtatcgccg ggtaccacca tttttttgaa gcgggcattg tcgattttca ggaaataggt
      421 cagataattt tcagggtctg gaacggtgtt gagggcaaag atgccgccgc attgtgccat
      481 ggcttctatc tgcaatactc cgggcataac aggttttcct ggaaaatggc cggtaaaaaa
      541 gccttcgttg atggttacat ttttaatgcc tattaccatt ttgtcgtcca tttccagaat
      601 tttatcgagg agcaaaaagg gtggccggtg tggcaagata ttctgaatct gtatgttgtc
      661 gtacaaaggc gttttggtca ggctgggtaa actaaccgaa gctttgtggg gtgatctgga
      721 gttaactccc accgtttcag ctatttttct ggcaaattct atatttgaaa aatggccggg
      781 gcgttttgcg atgatgtggg cgttgagtgt gccgccaagc aacgaaaggt cgccaatcag
      841 gtcgagaagc ttgtggcgtg ccggctcatt ctgaaaatgc agttctacgt tgttgagtat
      901 gccttcttta agtacgctta cttttggttt gttgaaaagc gtcgccagcc tgtcgagttc
      961 ttcctgagag attattttgt tgacaaaaat gatggcattg tcgaggtcgc cacctttgat
     1021 gaggttgttc ttcagcagaa attccagttc gtgtaaaaag acaaaagtgc ggcagggtgc
     1081 aatttccttt tcaaagtcaa cgatatcatc aagcatggca aactgctttc cgggaatatc
     1141 cgagtcgtaa tcaatcaacg tggtgatttt aaacgtgtcg gaagggatgg ccaggatttc
     1201 aaccttgttt ttttcgtctt tgtaaagaac gttcgtgttg aggctgatta cttttttcag
     1261 ggcatcctgt tctatggtgc cagcggtttt cagcagttcc acatatttta tcgcgctgcc
     1321 atccaaaatc ggggtttcgc agctgttgat ttcaataagt acattgtcga tggataatcc
     1381 tgaaagtgcc gccatggtat gttcaatagt atgaacgccc actccgtttt cttcgatgct
     1441 ggtgcctctg gaagtgtctt tgacattgcc taatacggca tgtaccatgg gctggttttc
     1501 caggtcaatg cgtttaaata ctattccatg attttcggga gccggatgaa aggtaatgat
     1561 aacattattt ccagtgtgga gtcctttacc ggtaagactt acaggtgatg ctacagttct
     1621 ttgctttgcc atattgaccg gcaaaattaa taaaatcctt tttatagaca aataaaaaaa
     1681 ttgctgtttt aaacgatgtt attaacaaga tgaattaccg aaatatggta tatatcagta
     1741 aatcagtgaa atggtagcat gagcaggtct atttcctgga aaggaatatg aaataagtcg
     1801 ctgatatact tgctggtaag tatgccactg aacatatagg tgcccatgcg gacacttttt
     1861 tcgttgcgca agaaattttc aataccacct tcttcggcaa tttgcaatat taccggtgcg
     1921 aaaaaattac ttaatgctgt ggaggctgta tggggaactt tcgaagggat attgggtacg
     1981 caaaagtgtg tgatgccata ttttttgaat accggttgtg tatggtttgt agcatgcgaa
     2041 gtttcggaac acccgccatt atcgatgctg acgtcgatga ttaccgatcc gaacggcatt
     2101 tctcttacca tatcttcggt gatgatgcag ggagtgcggc catttacagg gttgagcgcc
     2161 cctat
//
LOCUS       DAOSFP010000383         2155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_50587_length_2155_cov_4.736190, whole genome shotgun sequence.
ACCESSION   DAOSFP010000383 DAOSFP010000000
VERSION     DAOSFP010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50587_length_2155_cov_4.736190"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLI16_09720"
     CDS             complement(<1..292)
                     /locus_tag="PLI16_09720"
                     /inference="COORDINATES: protein motif:HMM:NF025118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOH84876.1"
                     /translation="MFYLKIFDTSQRMMGLAQEIITVSQLNENIKYILEETFGFVWVE
                     GEISNLRRPQSGHVYFTLKDDKSQVRAVYFKQFGRYRQGVSFELEEGLHILCR"
     gene            391..1407
                     /locus_tag="PLI16_09725"
     CDS             391..1407
                     /locus_tag="PLI16_09725"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOH84877.1"
                     /translation="MKKKNLLNKPVEHIDIKSINTVKMINAMGKMSFSARDLACAADI
                     YDRMLREKNCAIILTIAGSTSAAGCMQVYVDLVKNNMVDVIVATGASIVDMDFFEALG
                     FKHYQGTPFVDDKLLRKLYIDRIYDTYIDEEQLQACDRTIKKIADNLEPRPYSSREFI
                     AEMGKYLKKHSKKKNSLVQAAYENNVPIFCPAFSDSSAGFGLVLHQYERKRNHITIDS
                     VKDFLELTMIKIKAGKSGLLMIGGGVPKNFAQDTVVCAEVLGKKVPMHKYAVQITVAD
                     VRDGACSSSTLKEANSWGKVDSSCEQMVYAEATTVLPLLASYAYHKGNWKKRKKWQLA
                     KIFD"
     gene            complement(1778..1969)
                     /locus_tag="PLI16_09730"
                     /pseudo
     CDS             complement(1778..1969)
                     /locus_tag="PLI16_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          634 a          447 c          418 g          656 t
ORIGIN      
        1 cccggcaaag aatatgcagg ccttcttcca gttcaaaaga aacaccctgc cggtaacgcc
       61 caaactgctt gaaatagaca gccctgactt ggcttttgtc atccttgagc gtaaaataaa
      121 catgccctga ctgcggccgg cgcagattgg atatctcccc ttccacccaa acaaaaccaa
      181 aagtttcctc taggatatat ttaatatttt catttagttg tgaaacagta attatttcct
      241 gcgccagccc catcatgcgt tgtgaagtat caaatatttt caggtaaaac aagtttgatt
      301 ttgcccccta acaaacgact tcagattgac ttttaatcat ccttgttcta ataatctcct
      361 caggtattaa atcaaggaga atcaaactta atgaaaaaga agaacctact gaataagccg
      421 gtggaacaca ttgacatcaa atccataaac accgtgaaaa tgattaacgc catggggaaa
      481 atgtcttttt ccgcacggga tctggcttgt gccgccgata tttacgacag aatgctcaga
      541 gaaaagaact gcgcaattat acttactatc gccgggtcaa cctccgccgc cggctgtatg
      601 caggtatatg ttgatctggt gaaaaataat atggtggacg taatcgtggc taccggcgcg
      661 tcaattgtgg acatggactt ttttgaagcg ctgggtttta agcattacca gggcacccct
      721 ttcgttgatg acaaattgct gcgtaaactt tatatcgatc gtatctatga tacttatata
      781 gacgaagaac agctacaagc ctgtgatcgc acaattaaga aaatcgccga caatctggaa
      841 ccgcgtccct attcttcacg tgagttcatt gctgaaatgg gaaaatattt aaaaaagcac
      901 tccaaaaaga agaattcact cgtgcaggcc gcatatgaaa acaatgtgcc gattttctgc
      961 ccggcatttt ccgacagttc ggcgggtttc ggacttgttc tgcatcaata cgaacgcaaa
     1021 agaaatcata ttaccatcga ctcggtgaaa gacttcctgg aactgaccat gatcaaaatc
     1081 aaagcaggta aaagcggttt attaatgatc gggggcggcg ttccaaaaaa tttcgcccag
     1141 gatacggttg tctgcgccga agtactgggc aagaaagtgc cgatgcataa atacgcagtt
     1201 cagataaccg tggcggatgt gcgcgatggc gcctgctcca gttccacgct taaagaagca
     1261 aattcatggg gtaaagtgga ttcgtcatgc gaacaaatgg tttatgctga agctaccact
     1321 gttttgcctt tgcttgccag ctacgcttat cataaaggaa actggaaaaa acgtaaaaaa
     1381 tggcagctgg caaaaatctt tgattaataa aatgaaaaga tgagggggat tgaacagata
     1441 ttataagtaa tctgttttat tgtatatttt tgctattcga tttttgtaca tacacccaaa
     1501 aatacaccaa aaaatataga ctgtaatctt actctgtacg tctctttatt tttcttttgc
     1561 ttttcgattt gtcaatttta tttttttgat aattgacttc acctacaagg tcacaatttc
     1621 taatttggta ttagagtgat gattaccact tcaaatatgc ttcctgctgc agcaggaaat
     1681 tacggacaca tctattttcc ttttgttccg tttattatac taaaatccta acttaataaa
     1741 tggaccggtt ttcgaggggc aggtcacatt ttatttttca attcagatat gcctctggtg
     1801 atggaggacg ataattcaat gaactatgtg gcttgacagt gttgtattcc atacgccatt
     1861 gttcaattaa ggttttcgct tcttttaatg tatagaagat ttcaccatta agtaactcat
     1921 ctctgaattt cccgttgaaa ctttcaatat agccgttttc ccaggggctg cattttttat
     1981 gtttaaccac tgcctttatt ttttttgttg tttttcaaaa ataaaagtcc tatattttca
     2041 cgaacagggg gtgtctttat gaaaaaaatt accgccttaa ttatcttatt attaattatc
     2101 ttcagctctg accttctttg cgcggccacg ttttctcagg ccgacctgac gggca
//
LOCUS       DAOSFP010000384         2151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_50735_length_2151_cov_3.203721, whole genome shotgun sequence.
ACCESSION   DAOSFP010000384 DAOSFP010000000
VERSION     DAOSFP010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50735_length_2151_cov_3.203721"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLI16_09735"
     CDS             complement(<1..614)
                     /locus_tag="PLI16_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HOH84878.1"
                     /translation="METKKSGFNTKLIHAGAYEDEFGSATVPIYQTSTFKFKNAQHGA
                     DCFSGKTEGFIYSRIANPTIQALENNIAALENGYKGIATSSGMGAINCVYMALLGAGN
                     HIVSSDAVYGPARGVLEQDYARFGVEATFVNTANIENIKAAIRPNTKVLYIETPANPT
                     MDITDIAACAEIARAHNLILVVDNTFSSPYLQKPLDLGADVVLHS"
     gene            complement(1022..>2151)
                     /locus_tag="PLI16_09740"
     CDS             complement(1022..>2151)
                     /locus_tag="PLI16_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84879.1"
                     /translation="SVRSMTVTQDHLFANDESHVFRLIGADNWNDFSPNISFDFFSSL
                     SHMNDTIVLSVENNTPPDAPFIMYSPDSGSSWQHINSPPFAGDDPYRIYCSNHRIYAH
                     ENEIMYYTGNLGNTWTEISLPSQYCNQFNDFIVFHDTPYAAACGNGQLLKLDNNQQWV
                     ISNNGLPADREPIALAASDSALYTYINVHGMYVSHDNGSNWSYANYGLNTDYSINDYA
                     GYGELFFVTTNKGVFVTDDYGQNWSGCNDGLKNTNAGAIKFFHDTLYVSTLSNDYAGP
                     GNGIWKCAISDVHTSLPGYQQLSSSYFFFPNPAKGKIHLANSLEEADYAIFDMTGKQV
                     RTGHIREKEAIDISKINNGMYLIRLLIKDTFHIAKLVINKD"
BASE COUNT          554 a          440 c          509 g          648 t
ORIGIN      
        1 gagtgcaata ccacatcggc tcccagatca agaggtttct gcaagtatgg cgatgagaaa
       61 gtattgtcaa ccacaaggat taagttatgt gccctggcta tttcagcgca ggcggcgata
      121 tcggtgatat ccatggtggg attggccggt gtttcgatgt agagcacttt ggtattgggc
      181 ctgatggctg ccttgatgtt ttcaatgttt gcagtattta caaaggtggc ttccacgcca
      241 aaacgtgcgt aatcctgttc gagcacaccc cgtgcagggc catacaccgc atcggagctg
      301 acaatatggt tgcctgcacc caataatgcc atatacacgc agttgattgc tcccataccc
      361 gaactggtag ctatcccttt gtaaccgttt tccagggcgg ctatattgtt ttccaacgcc
      421 tgaatggtgg gattggcaat tctgctgtaa ataaatccct cggtttttcc cgaaaaacaa
      481 tcggcgccgt gttgggcgtt cttaaattta aatgttgacg tttgataaat aggcacggta
      541 gcgctgccaa attcgtcttc gtaagctcct gcgtgaatca gcttagtgtt aaatcctgat
      601 ttttttgttt ccattataat tgttatagtt taaattgtta ttaaaaagtt tccttgtttt
      661 tattgtttgt tacagctttc gtggctggtg catccttttc cgctgccggt gatgttgttt
      721 ttgaggaatt tgcctgcaag ttcctttaca tctcggctac agcctctgta attcaacctt
      781 ggtgtcctgt gtcaatgttt tgaaaagtgt tcaaaaaaat tttgcgcaaa ggtaatgaac
      841 gtgtgtgcat aatacaaaca aatgcctaaa aaaagtatgc gatatgccgg atcgaggttg
      901 gggaaacggg attacctata agaatcaaaa aagattacaa tatatgcggg aagcggttga
      961 cgctgcggtc ttttcattgc ttggtgtcaa taataatcac gtcttaatga tttataaaaa
     1021 attaatcttt attaattacc agttttgcaa tatgaaatgt gtctttaatc aataggcgga
     1081 taaggtacat gccgttattg attttagaaa tatctattgc ctccttttcc ctgatatgtc
     1141 ctgtgcgcac ctgtttcccg gtcatgtcga aaattgcata atcagcctct tccaggctgt
     1201 ttgcaaggtg aattttgcct ttggcaggat tggggaagaa gaaataagat gacgataatt
     1261 gctgataccc gggtagcgaa gtatgcacat cgctgatggc acatttccat atgccattgc
     1321 ccggaccggc gtagtcgttg ctcagggtgg atacatacaa tgtgtcgtga aaaaacttta
     1381 tggcgccggc attggtgttt ttcaatccgt cattacatcc cgaccagttt tgcccataat
     1441 catcggtaac aaatacacct ttgttggtcg taacaaaaaa cagttcccca tacccggcat
     1501 agtcgtttat tgaataatca gtattcaatc cgtaattggc atatgaccag ttgctgccat
     1561 tatcatgact tacatacatc ccgtgtacgt tgatgtacgt gtataaggcg ctgtcggaag
     1621 ctgccagggc aatgggttca cggtctgccg ggaggccgtt gttgctgatg acccattgct
     1681 ggttattgtc cagcttcagc agttgcccgt taccgcatgc ggcggcataa ggagtatcgt
     1741 ggaatacgat aaaatcattg aactgattgc agtattgcga aggcaggctg atctctgtcc
     1801 aggtgttccc taagttgccg gtataataca taatttcatt ttcatgggca tagatccggt
     1861 ggttggagca ataaattctg tacggatcgt cgccggcaaa aggcggactg ttgatgtgct
     1921 gccagctact gccgctgtcg ggactataca tgataaaggg ggcgtccggg ggcgtgttat
     1981 tttcaacaga taaaacaatg gtatcgttca tatggctgag cgaactgaaa aaatcaaagg
     2041 agatattagg ggaaaaatca ttccagttat ctgcgccaat cagccggaat acatgacttt
     2101 cgtcgttggc aaacaaatga tcctgagtaa cagtcattga ccttacggat g
//
LOCUS       DAOSFP010000385         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_51950_length_2118_cov_2.869123, whole genome shotgun sequence.
ACCESSION   DAOSFP010000385 DAOSFP010000000
VERSION     DAOSFP010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51950_length_2118_cov_2.869123"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1572)
                     /locus_tag="PLI16_09745"
     CDS             complement(<1..1572)
                     /locus_tag="PLI16_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOH84880.1"
                     /translation="MKKLFSLHNIYFALLIRIALLLILFTLCRIVFYLFNTASFSVSP
                     GELFLLLIYGFRFDLSSIILVNSLYIFFQTIPFDFRSNKTYQKILEIIFYVTNAIALL
                     LNCADIAYFKYLFRRTNYDIIKSIFIGDDFLTVFWEYVADYWYVVVVWLLIIALMVFL
                     YKKTKRVLILSNQTYGQYLQAALVFIAVMGLSVVGFRGGFQLRPISLVTAGEYTTADN
                     VPLVINTPFSIYCTVQKQPLTEKKYFQDDEAKRIFNPVKHNYRKDAPMHRLNVAIIIV
                     ESLSKEYIGVLNQDMEDRKYTGYTPFLDSLISESLCFDNCFANSKRSIEAIPSILSGL
                     PSLMEDAYLTSAFSGNKINSLPLLLKKYGYASMFFHGGKNGTLNLDAYAKMAGFDSYY
                     GKKEYNNDADYDGNWGIYDEEFLKFAVNTLDTSRQPFITAIYTLSSHHPYSIPKKYRN
                     KFKKGMLEIHESIMYTDYSLKEFFKACSSREWYDSTLFVITADHASVAGTPYYLNNAG
                     SYAIPLIFYMPGSTLK"
     gene            complement(1639..1965)
                     /locus_tag="PLI16_09750"
     CDS             complement(1639..1965)
                     /locus_tag="PLI16_09750"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HOH84881.1"
                     /translation="MRKISVIAVLIIVLTSMVSCKKNNSGDTTAPVITLIGSPQVYVD
                     KGTSYTDEGATAYDETDGDITSRIVVVNPVDANTEGTYYVSYDVTDEAGNKATEVKRK
                     VVVMVF"
BASE COUNT          648 a          415 c          427 g          628 t
ORIGIN      
        1 cttcagcgtg ctgccaggca tataaaaaat cagcggaatg gcgtacgagc cggcgttatt
       61 caaataataa ggggttcccg ccactgaagc atgatcagcg gtgataacaa acagggtgct
      121 gtcgtaccat tccctggaag agcaggcctt gaaaaactct tttaatgaat agtcagtgta
      181 cataatgctt tcgtgaattt ccagcatccc ttttttaaat ttattacggt attttttcgg
      241 aatggaatac ggatgatgcg atgataaagt gtaaatggca gtaataaatg gttgccggga
      301 agtatccagg gtattgacag cgaattttaa aaattcttca tcataaattc cccagttgcc
      361 gtcataatca gcgtcgttat tatattcttt ttttccgtaa taagaatcaa aaccggccat
      421 tttggcataa gcatcaaggt ttaaggtccc gttttttccc ccatgaaaga acatggaagc
      481 ataaccgtat tttttcaata acagtggtaa ggaatttatt ttgttgcccg agaaagcgga
      541 ggtaagatag gcgtcttcca tcagcgatgg caagcctgat aaaatggacg gtattgcttc
      601 gattgaccgc ttgctgtttg caaaacaatt atcgaagcaa agactttcgg atatcagcga
      661 atcgagaaaa ggcgtatagc ctgtgtattt cctgtcttcc atatcctggt tcaacacacc
      721 gatatattct tttgacaggc tttcaacaat aattattgcc acgtttaacc tgtgcatcgg
      781 cgcatccttc ctgtagttgt gttttacagg attgaatatt ctttttgcct catcatcctg
      841 aaaatatttt ttttcggtaa gtggctgctt ttgcacagta caatatattg agaaaggggt
      901 gttgatgacc aacggtacat tatctgctgt cgtatattct ccggcagtca caaggcttat
      961 gggccttaac tgaaaaccac cgcgaaaacc aaccaccgac agtcccatca cagcaataaa
     1021 aacaagggca gcctgcaggt attgcccata ggtttggtta cttaatatca acacacgttt
     1081 tgttttttta tatagaaaaa ccatcagcgc aataatcagc agccacacca ctaccacata
     1141 ccagtaatcc gcaacatatt cccagaaaac cgtaaggaaa tcatcaccga tgaaaattga
     1201 ttttatgatg tcgtaattgg tacgtcggaa caaatactta aaataagcaa tgtcggcgca
     1261 gtttagtaac agggcaatag cgtttgtaac gtaaaagatg atttcaagga ttttctgata
     1321 cgttttgtta cttcggaaat caaatgggat tgtttgaaaa aaaatataca gggaatttac
     1381 gagtataatg gacgaaaggt caaacctgaa gccatagatc aacaataaga ataactcacc
     1441 tgggctcacc gaaaaacttg cggtattgaa aagataaaac acgatacggc aaagcgtaaa
     1501 taaaataagc aagagggcga tccttattaa aagcgcaaaa taaatattat gtaaactaaa
     1561 aagttttttc atggaccgca aatgtaatca aaaaggaatt tttcaggata gggtttctct
     1621 gaaaattgac agcccgtatc agaataccat gaccaccact ttacgtttta cttctgtggc
     1681 tttgttgccg gcttcatcag ttacgtcgta ggatacgtaa taagttcctt cagtgttggc
     1741 atccacagga tttacaacga cgatccttga tgttatgtct ccgtcggttt catcataggc
     1801 ggtagctcct tcatcggtat aggatgtccc cttgtcaaca tacacctgcg gtgagccaat
     1861 cagtgtgatt accggtgctg tagtgtcgcc ggaattattt tttttgcagg aaaccatact
     1921 ggtcagaact attattaata cagctatgac agaaattttt ctcatgttgt tttttttgta
     1981 atttataaaa atcaatttcg gcaatctgct acaatctttt atataccatg tcgccggttg
     2041 tactagttcc ggatacggaa tcgctgtaat ggatttcaaa aagtgtggta tcgctgatgc
     2101 ttgaaacaaa tccattgc
//
LOCUS       DAOSFP010000386         2109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_52268_length_2109_cov_3.380721, whole genome shotgun sequence.
ACCESSION   DAOSFP010000386 DAOSFP010000000
VERSION     DAOSFP010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52268_length_2109_cov_3.380721"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..613)
                     /locus_tag="PLI16_09755"
     CDS             complement(23..613)
                     /locus_tag="PLI16_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOH84882.1"
                     /translation="MTLPEKLKDYRVILASASPRRHSLLKGLGIDFEVVVRETRETFP
                     EYLQREEIPVYLAELKSAAYNPDEFGPKTLVITADTIVWLDGCIIGKPENKEHAISLL
                     KQLSGKCHEVYTGVCLKTKKTIHCFHACSKVSFRTLSDEEILWYVNHYQPFDKAGAYG
                     AQEWIGFAGIEHIEGSFYNVMGLPTQKLYVELEKIL"
     gene            complement(615..1565)
                     /locus_tag="PLI16_09760"
     CDS             complement(615..1565)
                     /locus_tag="PLI16_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOH84883.1"
                     /translation="MRHYFKLIRPVNLLIIALTQYLMLFWIIKPFLAENSCGLQMSHL
                     DFALFVLANLLLAAAGYAINDYFDIEADSINKPGKNVLENKIPRRHAYRMNIIMNSLA
                     GIIGFCCAYKVGYIMLGFFFVVIALALYYYSLKYKRQFLTGNIVVALMTAYAVFSVWL
                     FQFFAIFSLAKAGDPYIYIHTIDARKEISYFILGFAVFAFLLTLLREFIKDIEDVRGD
                     EAVGCNTLPIKKGMPAAQRWVLVLSVIIIILLAVCQALLFTTQTALSLYLVVIQAMLV
                     YLIIKVKRARDVSEFHFLSMFAKIIIVAGLLSTQVLYSYF"
     gene            complement(1650..>2109)
                     /locus_tag="PLI16_09765"
     CDS             complement(1650..>2109)
                     /locus_tag="PLI16_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84884.1"
                     /translation="FEINTKDVEEYYLTSVIRGFYNYLFRRFILKNSFGFVAVTHELA
                     NYVNRHKEIIPLNTIIKPPSAELRPGQKDSDSLPEYDLLDKILFGYIEQSLSPEQLIA
                     QGCDPALVHRVLKMVNTNEYKRYQSAPILRVSPKAFGMGRRMPVVGKYVS"
BASE COUNT          647 a          469 c          434 g          559 t
ORIGIN      
        1 taatgcaaat gtcaggcatt atttataaaa ttttttccag ctcgacatat aatttctggg
       61 taggcaggcc catcacatta taaaatgaac cttctatatg ttcgataccg gcaaacccta
      121 tccattcctg agcgccataa gcgccagcct tatcaaaagg ctgataatga ttcacatacc
      181 acagaatctc ttcgtcgctg agtgtgcgaa aactaacttt tgaacaggca tgaaagcaat
      241 ggatggtttt ttttgttttg aggcatacac cggtgtaaac ctcatggcat tttcctgaca
      301 actgttttaa caaactgatg gcatgctcct tattttcggg cttgcctatg atgcagccat
      361 caagccacac tatggtatcg gctgtaataa caagagtttt agggccaaat tcgtcaggat
      421 tataagcagc tgatttgagt tcggcaagat atacagggat ctcctctctt tgcaaatatt
      481 cggggaaagt ctccctggtt tcgcgcacca ctacttcaaa atcaatgccc aaccctttga
      541 gcaggctatg gcggcgcggc gaggcagaag ccagtatgac acggtaatct tttaattttt
      601 cgggtaacgt catatcaaaa ataagaataa agcacctggg ttgataacag gccggcaact
      661 atgataattt tagcaaacat gcttaaaaaa tgaaattccg aaacatctct ggcccttttt
      721 accttgataa taaggtaaac aagcatggct tgtatcacca caaggtacag gctcaatgcg
      781 gtttgcgtcg taaaaagcag cgcctggcac acagccagca atattataat aataacactc
      841 agcaccagca cccaccgctg tgctgccggc ataccttttt ttattggcag ggtattgcat
      901 ccaacggctt cgtcgccgcg tacatcttcg atatctttga taaactcccg taacaaggtc
      961 agcaaaaagg caaatactgc aaatccgagt atgaaataag aaatttcttt tcgggcatct
     1021 atagtatgga tgtagatgta agggtctccg gctttggcca gcgaaaaaat ggcaaaaaac
     1081 tgaaataacc acaccgaaaa cacagcatat gccgtcatca gcgccacaac aatatttccg
     1141 gtaagaaact gccgtttata tttcaacgaa taataataca gcgccagggc aattaccaca
     1201 aagaagaatc caagcatgat gtagcctacc ttataagcgc aacaaaaacc gatgatgcca
     1261 gccagagagt tcataatgat attcatccgg taggcatggc ggcgcggaat tttattttcg
     1321 agcacatttt tgccgggttt gttgatgctg tcggcttcga tgtcaaaata atcattgatg
     1381 gcataccctg cggcagcaag cagcagattg gcaagtacaa aaagtgcaaa atcaagatga
     1441 ctcatctgca acccgcacga attttcagcc aggaaaggtt tgatgatcca gaacaacatc
     1501 aggtattgcg taagggcaat aataagcagg tttacaggac ggataagttt aaaatagtgt
     1561 cgcatgcggt attttttgca aagataatct tatttgaaaa ttctttctta tgattcaaaa
     1621 atcacaagat gacaaaaaaa cctcccgctt catgaaacat attttcccac cacaggcatt
     1681 ctgcggccca ttccaaaagc cttgggggag acgcgcagga taggtgccga ctgatacctt
     1741 ttatattcgt tggtgtttac cattttcagc acacggtgca ccagggcggg atcgcaaccc
     1801 tgtgcgataa gttgttcggg actgagactt tgctcgatat atccgaaaag tattttgtcc
     1861 agcaggtcat actcgggcaa tgagtcagaa tctttttgtc cgggcctcag ctcagccgaa
     1921 ggcggcttga tgatcgtatt caatggaata atttccttgt gacggtttac ataatttgcc
     1981 aactcatgag tcaccgcaac gaagccaaag ctattcttta aaatgaacct cctgaagaga
     2041 taattataga aaccacgaat cactgaagtc aaataatact cttccacatc cttagtatta
     2101 atctcgaaa
//
LOCUS       DAOSFP010000387         2108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_52299_length_2108_cov_4.156357, whole genome shotgun sequence.
ACCESSION   DAOSFP010000387 DAOSFP010000000
VERSION     DAOSFP010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52299_length_2108_cov_4.156357"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="PLI16_09770"
     CDS             <1..611
                     /locus_tag="PLI16_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84885.1"
                     /translation="EKQPEKIVVSLEKEPVKEVEKKPEVKDEMVLFTKEPEKEEIKIS
                     NPVITVSEPPVSEKPNTIVTDNSEKELKISFDVKQSDTEFFVYTKKETAEASFVNEPA
                     GNGNTKTEETQKENFNTEEVERLNKNHPDRVANLRAMSLKIKNKDGLEEIENTPAYIR
                     RKVQLENNKPSSDSEISDLSIGSEDGDKPGIKKNPYIHDNPD"
     gene            619..1068
                     /locus_tag="PLI16_09775"
     CDS             619..1068
                     /locus_tag="PLI16_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOH84886.1"
                     /translation="MSLELKINEDIKQAMLAKNKDLLEALRAVKSALLLAKTEKADAV
                     VNEEAEIKILQKLVKQRRETAEIYASQNRKDLADIENFQADVIQKYLPEQMGEEELTA
                     IIKAIIAETGASSIKDMGRVMGAAAKKLAGKADNKTISEKVKLLLGL"
     gene            1389..1985
                     /locus_tag="PLI16_09780"
     CDS             1389..1985
                     /locus_tag="PLI16_09780"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOH84887.1"
                     /translation="MKKSAIIAIILLMIVSGKSFSQETKAQTKVQIKPALLVIDIQNV
                     YLSMVPEMEKGIALYYINSLIELFRSNGYPVIRIYHFDKESGPKPGSEQFEYPGTVLI
                     KDEDPKIIKTYGNGFTKTDLDKILREKGSNTLFLCGLSAVGCVLATWIGAQDHDYKAF
                     MVKDAIMSHNSDYTNNIEDMFNAVGYDIVKMILENSAH"
BASE COUNT          726 a          432 c          450 g          500 t
ORIGIN      
        1 cagaaaaaca accggagaaa attgtggtaa gcctcgaaaa agaacccgta aaagaagtgg
       61 agaaaaagcc tgaggtgaaa gacgaaatgg tgttgtttac caaagaaccc gaaaaagaag
      121 aaataaaaat aagtaacccg gtgatcactg tttccgaacc tcctgtcagc gaaaagccga
      181 atactatcgt tacggacaac agcgagaaag aattgaaaat ctctttcgat gtaaaacaaa
      241 gcgataccga gttctttgtt tatacaaaaa aggaaaccgc cgaagcttct tttgtaaatg
      301 aacccgccgg caatggcaat accaaaacag aggaaaccca aaaggaaaac ttcaataccg
      361 aagaggtaga gcggctaaat aaaaatcatc ctgacagggt ggccaatctg agggccatga
      421 gtctcaaaat aaaaaacaaa gacggcctgg aagaaattga aaatacgccg gcatacatcc
      481 gccgcaaggt gcagctggag aacaacaaac cctcgtccga ctccgaaatt tccgacctgt
      541 ctattggatc ggaagacggt gataaacctg ggatcaaaaa aaatccatac atacacgata
      601 acccagactg attttgttat gagcctcgaa cttaagatca acgaagatat caagcaggcg
      661 atgctggcca agaacaagga cctgctcgaa gccctgcgcg ctgtgaaatc ggccctgctg
      721 ctggcaaaaa ccgaaaaggc cgacgccgtg gtcaacgaag aagccgaaat aaaaattttg
      781 cagaaactgg ttaaacagcg ccgcgaaacg gcagagatct atgcttcgca aaatcgtaaa
      841 gaccttgccg atatcgagaa ttttcaggcc gacgtgattc agaaatacct gcctgaacaa
      901 atgggcgaag aagaattgac ggcaataatt aaagcaatca tcgccgaaac aggtgcctcc
      961 tctattaaag atatgggcag ggtgatgggt gcagcggcaa aaaaattggc cggcaaagcc
     1021 gacaataaaa caatttcaga aaaagtaaaa cttttgttag gcctctgata aacgccctgc
     1081 gggtgtgtct gttttccctt acttaccact accgcataaa ctccaattat tggggtcaat
     1141 aatgaaaaca atgccctgta ggtggcgaca taatgcttat gcgctgaaaa ctgagctatt
     1201 cctcacattt actgttatgc aaagaatgca actcgcaaaa ccattccttg tctaacgcct
     1261 gtatcttaca ttctcttcaa aaaaaccaca ttaaatattt tttaaaatag tcattattgt
     1321 ataaatttgt acaagacaca ggatgttttt tatataagtc cattaatgtt caattaatat
     1381 catgttttat gaaaaaatca gctattatcg cgattatcct gcttatgatt gtttcgggta
     1441 aatcattttc acaggaaaca aaagcacaga ccaaagttca aattaagcca gccttgctgg
     1501 tcattgacat acagaacgtt tacttaagca tggtgccgga aatggaaaag ggaatagcct
     1561 tatattatat caatagcctg attgagcttt tccgcagtaa tggctacccg gtgatacgaa
     1621 tttaccattt tgataaggaa tctgggccca aaccaggttc cgagcagttt gaataccccg
     1681 gcactgtatt gataaaggat gaggatccga aaattattaa gacttacgga aatggattta
     1741 ccaaaacaga tttggacaaa atcctcaggg agaaaggaag caatacttta ttcctttgtg
     1801 gattgagtgc tgtgggatgt gtgctagcaa cctggatcgg ggcgcaggac cacgattata
     1861 aagcctttat ggttaaggat gctattatga gtcataattc cgactataca aacaatatcg
     1921 aagatatgtt taatgcagtt ggctatgata tagtgaaaat gatactcgaa aactcagccc
     1981 actaaaaagg gagagatgat tgctgtgcca aacagcatca gtgcccattc agtaaaagac
     2041 cgggaggtta atctaccctg ttgtgtgtcg gattttcgca ccagatgtct tatctgcccc
     2101 aatcccgt
//
LOCUS       DAOSFP010000388         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_52449_length_2104_cov_4.530015, whole genome shotgun sequence.
ACCESSION   DAOSFP010000388 DAOSFP010000000
VERSION     DAOSFP010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52449_length_2104_cov_4.530015"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLI16_09785"
     CDS             complement(<1..662)
                     /locus_tag="PLI16_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84888.1"
                     /translation="MAINLVTGEIKIILEELTEQSNTISQYEGKIPQIELDIIMANIR
                     KLYERYGDLNELNKKPLTSKPINELPVAEKPVPQPAEDDIPEIKITVNPPQDLSAPVS
                     EEKTDLFDIKNEDSVEKQPEKQPEEKVEKKIEEKAPEPEPKKKLKTEKAKTGDLFSLA
                     DKETVADKFKETAPSVLEKISAEKNDKTVAEKIGKSTVTNLKSAIGINDKFLFINQLF
                     KG"
     gene            742..1329
                     /locus_tag="PLI16_09790"
     CDS             742..1329
                     /locus_tag="PLI16_09790"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOH84889.1"
                     /translation="MFIEKTVETHKKGFIEVICGSMFSGKTEELIRRLKRAKYARQRV
                     EIFKPAVDVRYDVENVVSHDENVIHSTPVESASQILLLTADVDVVGIDEAQFFDAEIV
                     NVCNMLANRGIRVIVAGLDMDYLGNPFGPMPALLATAEYVTKVHAICLHCGDLAHVSH
                     RMIENDKLVLLGETDTYQPLCRSCYNKAIKEAEKG"
     gene            complement(1330..1695)
                     /locus_tag="PLI16_09795"
     CDS             complement(1330..1695)
                     /locus_tag="PLI16_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84890.1"
                     /translation="MKIFLPKNVLALLLVASLGVLVFYGCKKESTDCTAIILAKQLND
                     TMVVVSYADIIIAPQYQDVRVEGKTDIAGRFEHAFKYEGILDVIVFKPIEGTPDSLIG
                     RGVIRLKPGETTTKTIFLK"
     gene            1790..>2104
                     /locus_tag="PLI16_09800"
     CDS             1790..>2104
                     /locus_tag="PLI16_09800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOH84891.1"
                     /translation="MKKIIIFFSCLFMLSVAFSQGDQAVSEKKFLLKTTYGDITLKLY
                     NDTPQHRDNFTKLVNSGFYNGSIFHRVINKFMIQGGGAKGGTENAGYTVPAEILPQHF
                     HKF"
BASE COUNT          560 a          412 c          441 g          691 t
ORIGIN      
        1 tcgcccttaa ataactgatt gataaacaaa aacttatcat taatgccaat agcgcttttc
       61 aggtttgtaa ctgtagattt gccaattttt tcggccaccg ttttatcatt tttttctgct
      121 gaaatttttt cgagaaccga cggtgccgtt tccttaaact tgtcggcaac ggtttccttg
      181 tcggccaatg aaaagaggtc gcctgttttt gctttttcgg tttttagctt ctttttcggt
      241 tcgggttcgg gagctttttc ctctatcttt ttttcaactt tttcttcggg ctgtttttca
      301 ggctgttttt cgacgctgtc ctcatttttt atgtcaaata aatctgtttt ttcttcggat
      361 accggggcgg ataaatcctg gggcggattt accgtaattt ttatttcagg aatgtcgtct
      421 tctgcaggct gcggcacggg cttctctgct acaggcagtt cattgatggg cttggaagta
      481 agcggttttt tgttcagttc attgaggtct ccataccttt catatagttt tcggatattc
      541 gccatgataa tatcgagttc gatctgcgga atttttcctt cgtattggct gatagtattt
      601 gattgttccg tgagttcttc cagaattatt tttatttctc ctgtaacaag attgattgcc
      661 atagttgaaa aattaaaggt taatattaaa tttgcagtat tttacaggca taaaattata
      721 aaaaataaat acgctgcgct gatgtttatt gaaaaaacgg ttgaaacaca taaaaaaggc
      781 tttattgaag tcatttgcgg gtcgatgttt tcgggaaaaa ccgaggaact tatccgccgg
      841 ttgaaaaggg ccaaatatgc ccggcaacgt gtagagattt tcaagcccgc ggtagatgtg
      901 cgttatgatg ttgaaaacgt ggtttcgcat gatgaaaatg tgatacattc cacgccggtg
      961 gaatcggcct cacaaatact tttactcacc gccgatgtgg atgtagttgg catcgacgag
     1021 gcacagtttt ttgatgccga aattgtaaat gtgtgcaata tgttggccaa cagaggtatc
     1081 cgtgttattg tggccggcct ggatatggac taccttggaa acccttttgg ccctatgcct
     1141 gccctgctgg ccaccgccga atatgttacc aaggttcatg ctatttgttt acactgtggc
     1201 gatctggccc atgtttcgca ccggatgata gaaaacgaca aactggtttt gctgggcgaa
     1261 acagatacct atcaaccttt atgcagaagc tgttataata aagcaataaa ggaagcggaa
     1321 aaaggataat tactttaaaa agattgtttt ggtggttgtt tctcccggtt tcaggcggat
     1381 aacgcctctt ccgatgagag aatcgggagt gccttcgatg ggtttaaata caatgacatc
     1441 aagtattcct tcgtacttaa atgcgtgttc gaatctgccc gcaatgtctg tcttaccttc
     1501 cacacgtaca tcctgatact gaggggcaat aataatatcg gcataagaaa ccacaaccat
     1561 cgtatcgttg agttgtttgg caagaatgat tgccgtacaa tcggtacttt cttttttaca
     1621 gccataaaaa acaagaaccc ccaaacttgc aactaataac agggctaaga catttttagg
     1681 gagaaatatt ttcattgtta tatttttttt aattttgttc tgcgtaaatt aatacgcaaa
     1741 ttaaaacaat tttgttacaa aactacacaa atttacaaaa tttatcgtta tgaaaaaaat
     1801 tatcattttc ttttcgtgtt tgtttatgtt gtctgttgcc tttagccagg gcgaccaggc
     1861 ggtatccgaa aaaaagtttt tactgaaaac gacttacgga gatattaccc tgaaactgta
     1921 caacgacact cctcagcacc gtgataattt caccaagctg gtaaacagcg ggttttataa
     1981 cgggtctatt tttcaccgcg tcatcaacaa gtttatgatt cagggcggcg gagccaaagg
     2041 cggcaccgaa aatgccggct atactgtacc tgcagaaatt ttgccgcagc atttccacaa
     2101 attc
//
LOCUS       DAOSFP010000389         2101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_52576_length_2101_cov_3.639785, whole genome shotgun sequence.
ACCESSION   DAOSFP010000389 DAOSFP010000000
VERSION     DAOSFP010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52576_length_2101_cov_3.639785"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PLI16_09805"
     CDS             <1..264
                     /locus_tag="PLI16_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOH84892.1"
                     /translation="PLILLDHQPFFLETSAKNGVDIQFSGHTHNGQIFPLNLITRAIY
                     HPDWGYRKIGDTHFYVSCGVGTWGPPVRIGNYPEIVNAKIIFN"
     gene            781..>2101
                     /locus_tag="PLI16_09810"
     CDS             781..>2101
                     /locus_tag="PLI16_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HOH84893.1"
                     /translation="MKKLLIICVIFLGMAYISNAQTADRKWSIGLHGGLTQYSGDLGQ
                     SFYMANKGAYCFGGLSFSRYLNRFLDISLMGNYGNVGYKENESDNFFSGNMIHTNINI
                     RLKFLGNDKYKLSPYIFAGAGFTHFNKIKNNTTDNYIKKETLKFNNAARPVCGVGLTW
                     HIGDVVSMQLQELFIFSDYDRVDGQVGWERYNDNYLQHSLGLLFKFGKAKDTDNDGIP
                     DKDDKCPTVPGLAQFQGCPDTDKDGVTDADDKCPGTPEGVKVDAKGCPLDTDGDGIAD
                     YLDKCPDVKGLAKFEGCPDSDGDGVIDNDDQCPNTPERVKVDEKGCPYDKDGDGIPDY
                     KDKCPDVKGLPAYEGCPDRDGDGIPDNVDKCPDVKGIVANKGCPEVKEEVKKVFNQAL
                     QGIKFETGKDIIRPVSFPILDNVVKIMKENKEYNLIINGHTDNVGSDE"
BASE COUNT          672 a          420 c          412 g          597 t
ORIGIN      
        1 ccgctgattc ttttggacca tcagcctttt tttctggaaa catcagccaa aaacggcgtg
       61 gatattcaat tttctggaca tacgcataat ggacagattt tcccgctcaa cctgataaca
      121 cgagctattt accatcccga ctggggttat agaaaaatcg gcgacaccca cttttacgtt
      181 tcatgcggcg tgggtacatg ggggccgccg gtacgtattg gcaattaccc ggaaattgtt
      241 aacgcaaaaa ttatttttaa ttaaaaacaa gactttttca gtccgaaaaa ttcatatgaa
      301 tttttcggac ttagtatttt taaaaagaat tcctgattcc atgtttaata tttgatggtc
      361 taagcaggtc acttctttat ctcaacaaat agtaccaata agaaatttaa aatatgtacg
      421 ggctttattt tataatgctg atgcaacagt tgaacagggt taaatagtgc aaacgagtga
      481 agcgaagttt atactacatt attttccaat cggtataaaa acctttcttt ataattttcg
      541 taaaaagcca taattaaaat gatatttgtt ctaattttaa aaaatcatat tatttttaca
      601 tttcaaatta tagataaaac ttttcacaaa ttattattta aaaaattaac agttaaaaaa
      661 aattaaatta tttttaaaaa atgtaatatc aaaaattttt tataacttcg tttagtaaaa
      721 ttattacttt tgtttattac ttttgttcgt tttttataat aaataacaaa ttcttatact
      781 atgaaaaaat tacttatcat ttgtgttatc tttctcggca tggcttacat cagtaatgcc
      841 cagacagccg atagaaaatg gagcatcggg ttacatggag gcttaaccca atatagcgga
      901 gatttgggtc agagtttcta catggccaat aaaggcgctt attgctttgg cggattgagc
      961 ttttcgcgct atttaaaccg gttcctcgat atttctctta tggggaacta cggaaatgtg
     1021 ggttataaag aaaatgaaag tgacaatttc ttcagcggaa acatgatcca cacaaatatc
     1081 aatatccggc ttaaattttt aggcaacgac aaatacaaat tgtcacctta catctttgcc
     1141 ggtgcaggat ttactcattt taacaaaatt aaaaacaaca ccaccgacaa ttacatcaag
     1201 aaagaaacct tgaaatttaa caacgctgcc cgccctgttt gcggtgttgg tttgacctgg
     1261 cacatcggcg atgtggtcag catgcaactt caggaactgt ttattttcag cgactatgac
     1321 cgcgttgacg gacaagtggg ctgggaacgc tataacgaca attacctgca acacagcctg
     1381 ggacttttgt tcaaatttgg caaagcaaaa gataccgata acgatggtat tcctgataaa
     1441 gacgataaat gccccaccgt tccgggactt gcacagttcc agggctgccc cgataccgac
     1501 aaggacggtg taaccgatgc tgatgataaa tgtccgggaa cccccgaagg cgttaaggta
     1561 gatgccaaag gctgtccgct ggataccgat ggtgacggca ttgccgatta tctcgataaa
     1621 tgtcccgacg taaaaggtct ggctaaattt gaaggctgcc ccgattccga tggtgacggc
     1681 gttattgaca acgacgacca gtgccccaac actcccgagc gcgtgaaagt agatgaaaaa
     1741 ggctgcccgt acgacaaaga tggcgacggt attcctgact ataaagataa atgccctgat
     1801 gttaaaggtt tgcctgctta tgaaggctgc cccgacagag acggtgacgg tattcccgac
     1861 aacgttgaca aatgtcccga cgtaaaaggc attgtggcca ataagggctg ccccgaagtg
     1921 aaagaagaag tgaaaaaagt cttcaaccag gctttacagg gcatcaaatt cgaaaccggc
     1981 aaagacatta tccgcccggt atcgttcccc atccttgaca acgttgtgaa aatcatgaaa
     2041 gaaaacaaag aatacaacct cattatcaac ggccataccg acaatgttgg ctctgacgaa
     2101 t
//
LOCUS       DAOSFP010000390         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_53320_length_2081_cov_3.438302, whole genome shotgun sequence.
ACCESSION   DAOSFP010000390 DAOSFP010000000
VERSION     DAOSFP010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53320_length_2081_cov_3.438302"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..1512
                     /locus_tag="PLI16_09815"
     CDS             139..1512
                     /locus_tag="PLI16_09815"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOH84894.1"
                     /translation="MKRIFLIISLVVSIHAAVFSQPSDTAVIRNIFTEALTNYSAYDN
                     LRHLCKEIGGRIGGSPQSLRTAAWAKEVLMENGADTVYFLEHTVRNWKRGSKEVAKIV
                     KSSSGKHVLSVCALGGSVGTGKKGITARVIEVQNFDELKKLGKEKVNGKIVFFNRPAS
                     PATYSTFPAYGGAANQRVHGASQAALYGGIASVSRSLTLADHDYAHTGIMRYTDSLSK
                     VPAFAISTKSADLLSKLLKTDPKLELFLQSACFEKEDTASCSVIGELRGSEYPDEIIV
                     VGAHIDAWDNGEGAHDDGAGVVQAMEVLRIFKSLGIKLNHTLRVVEFMDEEMAQRGAR
                     KYAETALSSGEKHIAAIESDVGGFTPQGFCYDAGDEVTKRLDEWKPLLLEYGLWLYIQ
                     GHSAVDISFLEGNDIPLFGLMTDSQRYFDYQHAPSDVFEAVNKRELQLGAASMAALIF
                     LIDKYGF"
     gene            1542..>2081
                     /locus_tag="PLI16_09820"
     CDS             1542..>2081
                     /locus_tag="PLI16_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84895.1"
                     /translation="MSILLGLVFILSGLTKLYPIELFELTFIDLGVGTWKTAPFISRL
                     FIAMEFFLGIMLIFNIRLKKFTLKATLGLIIAFTIYLFIQMYFEGNKGNCKCFGNFLV
                     MTPLESIIKNLAMIAVAGLLLWAKHDITYRFQKLLMILGLLASLSLPFILNPPDFIAT
                     YQFQEETTGYKLNMDTIYNS"
BASE COUNT          600 a          468 c          456 g          557 t
ORIGIN      
        1 caaaggaacc tacgttatca aaatcactaa tggagagaat ataagcaccc ataaagttgt
       61 cattgtaaaa taatttattt tttttccaaa aaatacaaaa cctcgccgta attttacggc
      121 gaggttttgt ctatatatat gaaaagaata tttttaatta tatccttagt agtaagtatt
      181 catgctgctg tttttagtca gccatcagat actgctgtca ttcgtaacat tttcaccgaa
      241 gcgcttacca attattccgc ttacgataac ctaagacacc tgtgcaaaga gataggcggc
      301 aggattggcg gctctccgca atcgctcagg accgcagcat gggcaaaaga ggtgctcatg
      361 gaaaacggcg ccgatacggt atattttctg gaacataccg tcagaaactg gaaacgaggc
      421 agcaaagaag tagccaaaat cgttaagtca tcctcaggaa aacacgtttt gagtgtttgc
      481 gccttaggcg gatcggttgg cacaggaaaa aaaggcatca ctgcacgggt gatagaagta
      541 caaaactttg acgaactaaa aaaactcggc aaagaaaaag tcaatggaaa aatagttttt
      601 ttcaaccgcc cggccagtcc tgccacttat tccacctttc cggcttatgg cggcgctgcc
      661 aatcaacggg ttcatggggc ttcgcaagcc gccctgtatg gtggcatcgc ttcggttagc
      721 cgctccctga cactggccga ccatgattat gcgcacacgg gaataatgcg ttataccgat
      781 agcctcagca aagttccggc ttttgccatc agcaccaaaa gcgctgatct gctcagcaag
      841 ttgctgaaaa ctgatcccaa actggaacta tttcttcaat cggcctgttt tgaaaaagaa
      901 gataccgcct cctgcagtgt catcggcgag ttgcgcggct cagaataccc tgatgaaatc
      961 atcgttgtag gagctcatat cgatgcatgg gacaacggtg aaggtgcaca cgacgacgga
     1021 gccggtgtgg tgcaggccat ggaagtatta agaattttta aatcattagg catcaaacta
     1081 aatcacacac tgcgggtggt agaattcatg gatgaagaaa tggcccagcg cggagcccgc
     1141 aaatatgccg aaacagcgtt aagcagcgga gaaaaacata tagcagccat cgaaagcgat
     1201 gtgggcggtt ttaccccaca gggtttctgc tatgatgccg gcgacgaagt aaccaaaagg
     1261 ctggacgaat ggaaaccact gcttctggaa tatggcctgt ggctctatat ccagggtcat
     1321 tccgccgtgg acatcagctt cctggaagga aacgacatcc cgcttttcgg actgatgacc
     1381 gattcacaac gctactttga ctaccagcat gccccttccg atgtctttga agcagtgaac
     1441 aaacgcgaac ttcagcttgg ggcagcatcc atggcagccc tgattttttt aatcgataaa
     1501 tatggttttt aattatgata aaaaaaattg tttttagtgt cttgagcatc ttgcttggac
     1561 tggtatttat cttatcgggc ctgacaaaac tatatcctat tgaactgttc gagctgacat
     1621 ttatcgacct gggtgtgggt acatggaaaa ctgcaccctt tatctcccgc ttatttatcg
     1681 ccatggaatt tttcctcggc atcatgctga ttttcaatat ccggctcaaa aaattcacac
     1741 ttaaagccac tctgggactg attattgcct ttaccatcta tctgtttatt caaatgtact
     1801 ttgaagggaa taaaggcaat tgcaaatgtt ttgggaattt ccttgtaatg actccgctgg
     1861 aatctataat taaaaatctg gctatgatag ccgtggcagg attactgttg tgggcaaaac
     1921 atgatatcac ataccgtttt caaaaattgc taatgatact tgggctcctt gcttcactca
     1981 gcctgccttt tattctcaat ccccctgatt ttattgcaac ataccagttt caggaagaaa
     2041 ctaccggcta taagctgaat atggacacca tatataacag c
//
LOCUS       DAOSFP010000391         2076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_53534_length_2076_cov_3.007422, whole genome shotgun sequence.
ACCESSION   DAOSFP010000391 DAOSFP010000000
VERSION     DAOSFP010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53534_length_2076_cov_3.007422"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="PLI16_09825"
     CDS             <1..763
                     /locus_tag="PLI16_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOH84896.1"
                     /translation="GIAAGLTIGGKIPFAGTFANFATGRVYDQIRQCVAYSNKNVKIC
                     ASHAGITLGEDGATHQIMEDIGLMKMLPNMVVINTCDFNQTKAATKAAAHYTGPVYLR
                     FGRPKVPVFIPENEPFIIGKALMIEEGCDVSIFATGHLVWKAMQAMEILKAKGISAEL
                     INIHTIKPLDEEAILKSVSKTRCVVTAEEHMMNGGLGDSIAQLLSRKMPLPLEMVAVD
                     DKFGESGTPDELMVKYGLDTTNVVNAAMKAVQRKS"
     gene            827..1510
                     /gene="mtgA"
                     /locus_tag="PLI16_09830"
     CDS             827..1510
                     /gene="mtgA"
                     /locus_tag="PLI16_09830"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOH84897.1"
                     /translation="MAKGKKLKKILKILLWLLLIFLGLSILPVVIYKFIAPPITPLMV
                     LRGFETDKKTDYQWIPIEKMSPNLFQAALAAEDDTFLKHHGFDFRAIKLAYESNQKSK
                     RTKGGSTISQQTAKNVFLWPHRDFVRKGLEAWFTILIEFIWGKERIMETYMNVVEFGP
                     GVYGVEAASLKYFKKHASKLSKYEAASLISVLPNPHIYKVLTPSRYTQRYRGAIIRRM
                     GYIPKVKFE"
     gene            complement(1553..2038)
                     /locus_tag="PLI16_09835"
     CDS             complement(1553..2038)
                     /locus_tag="PLI16_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOH84898.1"
                     /translation="MGANTLVPFIDSTYATIIHREGRAIMGLSMGGHGALYLTIRHQQ
                     MFGAAGSMSGCLDLRPFSNNWDLSGRLGSIDTYPENWEENSVINMLGRIKNDSISIIT
                     DCGVDDVFISVNRQVHEKMISLNISHDYIERPGGHTREYWSSALPYQLLYFHNYFSKY
                     R"
BASE COUNT          597 a          458 c          488 g          533 t
ORIGIN      
        1 gggtatagcc gcaggcctta ccattggcgg gaaaatccct tttgccggca ccttcgccaa
       61 ttttgccaca ggccgtgttt acgaccagat aaggcaatgt gtggcttact caaataaaaa
      121 tgtcaaaatt tgtgcttccc atgccggcat caccctcggc gaagatggcg ccacgcacca
      181 gatcatggaa gatatcgggc tgatgaaaat gttgcccaac atggtggtca taaacacctg
      241 cgattttaac cagaccaagg ccgccaccaa agcggcagca cactatacag gacctgttta
      301 tctgcgtttt ggaagaccaa aagtgcctgt atttattcct gaaaacgagc cttttattat
      361 tggcaaagcc ctcatgattg aagaaggttg tgatgtgagt atttttgcca ccgggcacct
      421 ggtatggaaa gccatgcaag caatggaaat cctgaaggca aaaggtatca gcgctgagct
      481 gatcaacata cacaccatca agccgctgga tgaggaagcc attttaaaat ctgtcagtaa
      541 aacccgttgc gtggttactg ctgaggaaca catgatgaat ggcggcctgg gcgacagcat
      601 cgctcagttg ctttcgcgca aaatgcccct gccattggaa atggtggctg tggacgataa
      661 attcggcgaa agcggtaccc ccgatgaact gatggtcaaa tacggcctgg acaccaccaa
      721 tgtggtaaat gccgcaatga aggctgtaca gcgaaaatca taatgttttg gatgtaatgc
      781 atgtgcggca tgcgcctgta atcatgcaga caggatagaa tgaacaatgg caaaagggaa
      841 aaagctaaaa aagatactga aaatactgct gtggctgtta ttgatttttt tgggactctc
      901 catactcccg gtagtgatct ataagtttat tgctccgcca attacgccat taatggtact
      961 gcgcggtttt gagaccgata aaaagaccga ttatcagtgg ataccgattg agaaaatgtc
     1021 gcccaacctg tttcaggctg cccttgcagc ggaagatgat acctttctga aacatcacgg
     1081 gtttgatttt cgcgcgatta agctggccta tgagtcgaac caaaaaagta aaaggaccaa
     1141 aggcggaagc accatcagcc agcaaacggc caaaaatgtt tttctgtggc cgcaccgcga
     1201 ttttgtgcgt aaagggctgg aagcctggtt tacaattctc attgaattca tctggggaaa
     1261 agaacggatc atggaaacgt atatgaatgt tgtggaattt ggcccgggtg tttatggcgt
     1321 ggaagccgca tcattaaagt atttcaaaaa acatgcatca aaattaagca agtatgaagc
     1381 ggcctcgttg atctctgtac tgcccaaccc tcatatttat aaggtgctga caccatccag
     1441 atatacacag cgatacaggg gtgcaattat ccgcaggatg gggtatattc cgaaggtgaa
     1501 gtttgaatga atgattattt aggaaatgat accacaggtc aatatgtatt gtttacctgt
     1561 atttgctgaa ataattgtga aaataaagaa gttgataggg cagtgcacta ctccagtatt
     1621 ccctggtatg tccgccgggc ctttcaatat aatcatggct gatattcagt gaaatcattt
     1681 tctcgtgaac ctgccggttt acgctgataa aaacatcgtc cacaccacaa tcagtgatta
     1741 tcgatatgga gtcgtttttt atccggccca gcatattgat tacggaattt tcctcccagt
     1801 tttcggggta tgtatctatg ctgcccagac gcccggacag atcccaattg ttgctaaatg
     1861 gcctcaaatc caaacatccg ctcatgcttc cggcggcgcc aaacatttgc tgatgccgta
     1921 tagtcaagta taaagctccg tggccgccca tgcttaatcc cattatggca cggccttcgc
     1981 ggtgtataat tgtcgcataa gtgctgtcaa taaaaggcac tagtgtatta gcccccaaaa
     2041 tagtcggaca agattataga taaaaatcta taactt
//
LOCUS       DAOSFP010000392         2070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_53734_length_2070_cov_3.687345, whole genome shotgun sequence.
ACCESSION   DAOSFP010000392 DAOSFP010000000
VERSION     DAOSFP010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53734_length_2070_cov_3.687345"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="PLI16_09840"
     CDS             <1..1212
                     /locus_tag="PLI16_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84899.1"
                     /translation="ADKAEDFCKSIPSFSCSDTLELIRRDCHLGIYGNYLKNANKLFD
                     QKKFKEASAEVTRGILYQEKFSKYIADNSDALALKQKIGIEEYYDSMKKGKEKMLSKD
                     YRAAFVEFSNAKNLESDYPVQKDRLLPDLLKKSKLESLFLDIDDAEASVAANNLSLAR
                     STLRLVIDDQKVYGLTDNSALSVRIENLKKAIFSQECQNAQKDYDAKVDAAVKAELEK
                     SYITANDNYNEALQIVKKNTDCDINGEMAKKGQANTEKPAQYQQTLNLCNDRVKNNDY
                     LKAIEAYNKLTPYYHDNGIQDYGITHQPLHLYIASQHSGFVYYGLTWLTDAGELDNAM
                     YLLKQLRYRNVIKSTTKNQQLALARAYALNDVKNGVTTTPKVKVLEYTLGDKWYSYFE
                     KEYLKQIKKLK"
     gene            1379..1780
                     /locus_tag="PLI16_09845"
     CDS             1379..1780
                     /locus_tag="PLI16_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84900.1"
                     /translation="MIRKILLALALGALGLLAISCHKDNDIDEPEKPADFRDIYIGKY
                     QVKETINCYGPCGTCYSLRDTVISVNYGLTDSTLSVLGRDVYLDSNGFYSAYHYGLRF
                     WDDSISSNFMNGGLGCGRYESYKGYRFSNVP"
BASE COUNT          674 a          448 c          417 g          531 t
ORIGIN      
        1 gccgacaaag ctgaagattt ttgtaaaagc ataccttcat tttcttgttc cgacacgctg
       61 gaacttatcc gccgcgactg tcacctgggc atttatggca attacctgaa aaacgccaac
      121 aaattgttcg atcagaaaaa atttaaagaa gcctcggcag aagtaacccg tggtattttg
      181 taccaggaaa aattctcaaa atacattgcc gacaacagcg atgccctggc attaaaacaa
      241 aagatcggca tagaagaata ttatgattcg atgaaaaaag gcaaagaaaa aatgctgtca
      301 aaagactacc gggctgcctt tgtcgaattt tccaatgcaa aaaacctgga aagtgattac
      361 cctgtgcaaa aagaccggct tctccccgac ctgctgaaaa aatcaaaact cgagtcgctg
      421 tttctcgata tcgatgatgc cgaagcctcc gtcgcagcaa ataacctctc cctggcccgc
      481 agtaccctgc ggcttgtaat tgacgaccag aaagtttatg gactaacaga caactctgcc
      541 ctgtcggttc gtatcgaaaa cctgaaaaaa gccatcttca gccaggaatg ccagaatgct
      601 caaaaagatt acgacgcaaa agtggatgcc gccgtaaaag ccgaacttga aaaaagttac
      661 atcaccgcca acgacaacta caacgaagct ctgcaaatag tgaaaaaaaa taccgactgt
      721 gatatcaatg gcgaaatggc aaaaaaaggg caggcaaata ccgaaaaacc ggcacaatac
      781 cagcagacgc tcaacctgtg taacgaccgg gtcaaaaaca atgactacct gaaagccatt
      841 gaagcctaca acaaactcac gccttattac catgacaacg ggatacagga ttatggcatt
      901 acccatcagc cactccatct gtatattgcc tcacagcatt ccggatttgt atattacggc
      961 cttacctggc tcaccgatgc cggcgaactg gataatgcca tgtacctgct gaaacaactc
     1021 aggtaccgta atgtgataaa atccaccacc aaaaatcagc agttagcgct ggcgcgtgct
     1081 tatgccctga atgatgtaaa aaacggtgta accaccaccc ccaaggtaaa agtgctggaa
     1141 tatacattag gcgataagtg gtatagctat tttgaaaaag aatacctcaa gcaaatcaaa
     1201 aaactaaagt aagtaaccgg ctgatacctt ttaaggctcc ggagtacagc atctgtgtgt
     1261 acccgatgca tgccagatat cctaccgcca ttttcttgta aagcagtacg gacaaaaata
     1321 gtttttcttg tattcgggct aataagttcg taactttgta attactaaaa acatggacat
     1381 gatacggaaa atattattgg cacttgctct tggtgcactc gggttattag ccatatcgtg
     1441 ccataaagat aacgacatcg atgagcctga aaaacccgcc gatttcaggg atatctatat
     1501 tggaaaatat caggtcaagg aaactatcaa ctgttacggc ccttgtggta cctgctactc
     1561 attaagagac acggtgattt ccgttaatta cggcttgacc gattccactt taagtgtatt
     1621 gggccgcgat gtttatctcg actcaaatgg cttttacagc gcttatcatt atggtttaag
     1681 gttttgggac gacagcatct cttcgaattt tatgaacgga ggcctgggtt gcggccggta
     1741 cgaatcatat aaaggttaca ggttttccaa tgttccatag tttttaggaa tttctttgcg
     1801 ggcacctcaa aacttcaatt tattcgttat tcagtgtttg atattaaaag ggaataaatg
     1861 atgtaggaat ttcgattacc gaatatcgat tttgaaatgc agggccactc ttaaatcatc
     1921 attcgttaat cggtgtccga tattaataag aaataaaaga tgtacgaatg tcgattaacg
     1981 attttagatt tttgaagtgt aggaatactt ctcagatcat cattcgttaa tcggtgtccg
     2041 atattaataa gaaataaaag atgtacgaat
//
LOCUS       DAOSFP010000393         2068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_53826_length_2068_cov_3.239940, whole genome shotgun sequence.
ACCESSION   DAOSFP010000393 DAOSFP010000000
VERSION     DAOSFP010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53826_length_2068_cov_3.239940"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLI16_09850"
     CDS             complement(<1..340)
                     /locus_tag="PLI16_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose transferase"
                     /protein_id="HOH84901.1"
                     /translation="MVFDKTKINTTVQIILLLVLIYLVVFVKLGAFHMRLWDESMFAV
                     NTYEMINNGNYFANYFDGHPDICNTKPVLTLWAQIACVKLLGYNELAVRLPSAIAAAL
                     TILLVFVFLYR"
     gene            complement(393..1856)
                     /locus_tag="PLI16_09855"
     CDS             complement(393..1856)
                     /locus_tag="PLI16_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84902.1"
                     /translation="MKRIFLASLFAGFIIITFLLSSCKKDTQINDDPSVTLTLSTESI
                     LFDTVFSTVGSTTSMCYVKNPTDDNIVISSIRLANGDASQFSMNVDGVPAQNLSDVEI
                     GPNDSIFIFVKVTVDPQNSNSPMVIRDSIIFELNGNVQDIDLTAWGQDAHYIVADKTL
                     GGSLRYKIVAGENENITWTNDKPYLIYGYAVVDSTGKLNIEPGCRLHFYNNSGMWVYK
                     YGTLKVNGTIDQPVTFEGSRLEAAYKDLPGQWDRIWINEGSNDNIINYAVIKNGFIGI
                     QAETMSSAGSSRLILKNTVVKNMTGIGLLSRYYRIISGNCVFANCGGYAVALTTGGNY
                     DFRHCTLGNYYSYSSRQTPSLLITNYYLAGAYTYTGDLDSAYFGNCIIYGSNAEELET
                     DSVEDAAFNYKFEYCLLKSTNYSSDSRLVNCIFNQDALFKDYNNNDYTLSPGSAAIDA
                     ANINLIYFPVIDLVYDIKGRNRTLNPPPDMGAYDYIP"
BASE COUNT          601 a          406 c          440 g          621 t
ORIGIN      
        1 tcctgtacag aaatacaaat accagtaata tggtcagcgc tgcggcaata gcggaaggca
       61 aacgtactgc caattcatta tatccgagca gttttacgca tgcaatctgt gcccagagag
      121 tcaaaacagg ttttgtatta cagatatctg ggtggccatc gaagtagttt gcgaaataat
      181 taccgttatt aatcatttca taagtgttta cggcaaacat tgattcgtcc cataagcgca
      241 tgtgaaaagc tcccagcttc acaaacacaa caaggtatat cagcaccagc agaagtatta
      301 tctgaacagt ggtattaatc tttgtcttat caaaaaccat tatttgtcag gagatttttt
      361 attttaaaaa gttaacggct gacaacataa tatcagggta tgtaatcata agctcccata
      421 tctggcggtg gattcagcgt gcggttgcgg cctttgatat cgtaaaccaa atctatcacc
      481 gggaaataaa tcaggttgat attagccgca tctatcgctg cagatccggg acttagcgtg
      541 tagtcgttgt tgttgtaatc cttaaatagc gcatcctgat taaaaataca atttaccagc
      601 cggctgtccg acgaataatt ggtgcttttc agcaggcagt attcaaattt atagttgaat
      661 gcagcgtctt ctacactgtc agtttccagt tcttctgcgt ttgagccata aatgatgcag
      721 ttgccgaaat aggccgagtc aagatctccg gtataggtat aagcgccggc caggtaataa
      781 ttggtaatca acagcgacgg tgtctggcgc gaagagtagc tgtagtagtt gcccagggta
      841 caatgcctga agtcgtagtt gccacccgtg gtaagggcca cggcatagcc cccgcagttg
      901 gcaaagacac agttgccgct gatgattctg tagtatcgcg aaagcaagcc gatgccggtc
      961 atgtttttca caacagtatt ttttagtata agacggctgc ttccggcgct gctcatggtt
     1021 tcggcctgta tgcctataaa accgttcttg atgacggcat agttgattat gttgtcgtta
     1081 cttccttcat tgatccagat tctgtcccac tggcccggta aatccttgta tgcggcttcg
     1141 aggcggctgc cctcgaaggt caccggttgg tcgatggtgc cgtttacttt taaggtgcca
     1201 tatttataaa cccacatacc cgaattgttg taaaaatgga gtctgcagcc gggttctata
     1261 tttagtttcc cggttgaatc aactacagca taaccgtaaa tcaggtaagg tttatcgttt
     1321 gtccaggtga tattttcatt ttcaccggct acaattttat aacgaaggga gccccccagc
     1381 gttttatcgg caacaatgta atgagcatcc tggccccagg ccgtaaggtc tatgtcctgt
     1441 acgttgccat tgagttcaaa aataattgaa tctcttatca ccatcggaga gttgctgttt
     1501 tgagggtcaa cggttacttt tacaaaaata aaaatactgt cgttcgggcc aatctcaaca
     1561 tcactaaggt tttgagcagg gacgccatcc acattcatcg aaaactgtga agcatcacca
     1621 ttggccagac gtattgaaga aatcacaata ttgtcgtcgg tgggattttt gacgtagcac
     1681 atactggtag tggatccaac cgtcgaaaaa acggtgtcga acaatatcga ctcggttgag
     1741 agcgtaagcg ttacagaagg atcatcattt atttgcgtat cttttttaca ggaactcaaa
     1801 aggaaggtga taataataaa tccggcaaat aaggatgcta aaaaaattct tttcatcagt
     1861 tttttcttaa aaacgtcatt ttgttgatat tgtttcgcaa aagtaaatta ttatttttta
     1921 ttattcatgg caaagagaaa agagagatta ttttttttga tgtatgaaag aaaaaaagct
     1981 taacacattg ttaaattatt aaaaaaatat aaaaaacttg tattttatca ttttaagtcg
     2041 taatttagaa aaatagtttc tttattat
//
LOCUS       DAOSFP010000394         2065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_53942_length_2065_cov_3.786567, whole genome shotgun sequence.
ACCESSION   DAOSFP010000394 DAOSFP010000000
VERSION     DAOSFP010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53942_length_2065_cov_3.786567"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLI16_09860"
     CDS             complement(<1..481)
                     /locus_tag="PLI16_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84903.1"
                     /translation="MTKKILLYSFLFIVSFSLFSQKENAPFGSRSGAMGHASVTFTDF
                     WSVQNNQAGMAYYDKIAAGIYYENRFITKELGLKCFSLVVPVNKAGVFGVNVSSLGYR
                     LYNESKIGLAYGMSFGKNISAGVQLDYIYTHIGENYGNKGAVTFEAGIRAVLIKNLVI
                     "
     gene            complement(486..989)
                     /gene="purE"
                     /locus_tag="PLI16_09865"
     CDS             complement(486..989)
                     /gene="purE"
                     /locus_tag="PLI16_09865"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOH84904.1"
                     /translation="MKALVSIIMGSTSDLPVMEAAAKFFEDMQIPFEINALSAHRTPD
                     EVAVFAKNAHARGIKVIIAGAGMAAHLPGVIAAMTPLPVIGVPIKSSLEGLDSVLAIL
                     QMPPGIPVATVAINGALNAAILALQILALDNEELMKKNIAYKENLKAKIVKANEELSQ
                     LKYSYKV"
     gene            1077..1613
                     /gene="hpt"
                     /locus_tag="PLI16_09870"
     CDS             1077..1613
                     /gene="hpt"
                     /locus_tag="PLI16_09870"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOH84905.1"
                     /translation="MKRVTLHDKTFELSIPERDILQAVDHVAAQINKDYAGCEPVFLI
                     ILNGSFMFAADLLKKIDFNCETSFVKLSSYAGTESTHCVRELIGIEENLKGRRIIVVE
                     DIIDSGLTMEYLLGELKKVQPADVSIATFLLKPDALTRDIRIDYVGIKIPNDFIVGYG
                     LDYDGQGRNLPDIYKILP"
     gene            1624..>2065
                     /locus_tag="PLI16_09875"
     CDS             1624..>2065
                     /locus_tag="PLI16_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOH84906.1"
                     /translation="MFNIILFGAPGTGKGTQSENIINKYHLEHLSTGDIMREEIRKGT
                     QIGVLAKSYIDKGLLVPDSVIFKNLYYKASAIKNPKGFIFDGFPRTINQADVLDKVLI
                     KKQCPIALVLYLDVTEKESFNRIMYRSQRSNRADDNEEIIWKRIA"
BASE COUNT          610 a          441 c          441 g          573 t
ORIGIN      
        1 ctatcaccag atttttgatg aggacagccc tgatgcctgc ttcaaaggtg actgcgccct
       61 tgttgccata gttttcgcca atatgggtat agatataatc aagctgcact ccggccgaga
      121 tatttttccc gaaagacata ccgtaggcca ggccaatttt tgattcgtta tacagacgat
      181 aacccaggct ggatacgtta acaccgaaaa ctccggcctt gtttacaggc accaccagcg
      241 agaaacattt taatcccagt tctttagtga taaaacggtt ttcataatag attccggcag
      301 ctatcttatc gtaataagcc atgcccgcct ggttgttttg taccgaccag aaatcagtga
      361 aggttaccga tgcatggccc atggctccgg agcgactgcc gaaaggtgca ttttcctttt
      421 gcgaaaaaag agagaaagaa acgataaaaa gaaaggaata taatagtatt ttttttgtca
      481 tcggatcata ctttatatga atactttaat tggctgagtt cttcgttggc cttgacaatt
      541 tttgctttca gattttcttt gtaggcaatg ttttttttca tcagctcttc attatccaat
      601 gcaagaattt gtaaagcaag tatggcagcg ttgagggcgc cgtttatggc taccgtggcc
      661 acaggaattc ccgggggcat ttgcaatatg gccaacacgg aatctaatcc ttccagcgac
      721 gattttatag gcacgccaat caccggcaag ggcgtcatgg ctgcgataac tccaggcaaa
      781 tgtgccgcca tacccgctcc ggcaataatt acttttatgc cccgcgcatg ggcatttttt
      841 gcaaaaacgg ctacttcatc tggagtccgg tgggccgaaa gcgcatttat ctcgaaaggt
      901 atctgcatgt cctcgaaaaa ctttgctgca gcttccatca ccggaaggtc tgacgtgctg
      961 cccattataa tgcttactag tgctttcata agaaaattat ctacacaaag ataaaatatt
     1021 tataagattg tcgggaaaat tattgaaatt tgtactttag ccatgaaaat aattttatga
     1081 aaagagtaac ccttcacgac aagacttttg aactgtctat tccggaaaga gacatcctgc
     1141 aggcagtgga tcatgtggct gcacaaataa ataaagacta cgcaggctgc gaaccagtat
     1201 ttctgattat cctcaacggc tcttttatgt ttgccgctga cttgctgaaa aaaattgact
     1261 ttaactgcga aacaagtttt gtgaaactct catcctatgc aggcactgag tccactcact
     1321 gtgtcaggga gcttattggc atcgaagaaa acctgaaagg caggagaata attgttgtgg
     1381 aagatatcat agattcaggc ctgaccatgg aatacctgct gggtgaactt aaaaaggtgc
     1441 agcctgccga tgtgagtata gctacttttt tgctgaaacc cgatgccttg accagagaca
     1501 tcaggattga ttatgttgga ataaaaatac ctaatgactt tattgtgggt tatggcctgg
     1561 actatgacgg acaagggcga aacctgcctg atatttataa aattttacct taaaaccttg
     1621 actatgttta acattatact atttggagca ccgggaaccg gaaaaggaac acaatccgaa
     1681 aatatcatta acaaatacca tctggaacac ctgtccaccg gtgacatcat gagggaggaa
     1741 atccgcaaag gcacccagat cggggttctg gcaaaatcgt atattgataa aggcctgctg
     1801 gttcccgaca gcgtgatatt taaaaacctc tattataaag cttcggccat caaaaaccca
     1861 aaaggtttta tctttgatgg atttccccgt accatcaacc aggccgatgt gcttgacaag
     1921 gtactgataa aaaaacaatg tcccattgct ttggtgttgt atctcgacgt tacagaaaaa
     1981 gagtccttca atcgtataat gtaccgtagt cagcgctcaa accgtgctga cgataatgaa
     2041 gaaataatct ggaaacgtat tgctg
//
LOCUS       DAOSFP010000395         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_54211_length_2058_cov_4.679481, whole genome shotgun sequence.
ACCESSION   DAOSFP010000395 DAOSFP010000000
VERSION     DAOSFP010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54211_length_2058_cov_4.679481"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLI16_09880"
     CDS             <1..685
                     /locus_tag="PLI16_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH84907.1"
                     /translation="KEEVLHNINLGIEKGKTISIVGPSGAGKSTLVDLLPRFYDCTGG
                     ELLVDGVNVKDYVISDLRQLMGIVTQETILFNDTVFNNIAFGMESVSEEAVIQAAKIA
                     NAHDFIMQLENGYQSYIGDRGMKLSGGQKQRLSIARAVLLNPPVMILDEATSSLDTES
                     ERLVQEALVKLMQNRTSVVIAHRLSTVQFADEIIVLQNGKIVERGTHTELSALKGVYH
                     KLCEMQTFT"
     gene            685..1137
                     /locus_tag="PLI16_09885"
     CDS             685..1137
                     /locus_tag="PLI16_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOH84908.1"
                     /translation="MPLMNPIDERIFKLINKHHIFTLATAENNMPYCCTCFYAFMREK
                     NLFVFTSEYKTQHISHFLKQPVVAGSIALETRIIGKIRGLQFTGKMIELKDQELKEAR
                     RAYLLKFPYTKPFLKDTPFWGIVPAFYKLTDNRLGFGTKLIWSNLDNL"
     gene            1507..>2058
                     /locus_tag="PLI16_09890"
     CDS             1507..>2058
                     /locus_tag="PLI16_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HOH84909.1"
                     /translation="MEKKGKSEQTTYSHESALAATIEYFKGDTLAADVWINKYALKNR
                     DGELLEKTPDDMHRRIAREIARIEKKYPNPMSEELIFDLLKDFRYIIPQGSPMAGIGN
                     DFQISSLSNCFVIGNDTPSDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPQGSLVKN
                     SALTSTGIVPFMERYSNSTREVAQ"
BASE COUNT          624 a          458 c          439 g          537 t
ORIGIN      
        1 aaaagaagag gtgcttcaca acatcaacct cgggattgaa aagggtaaaa ccatttccat
       61 tgtggggcct tccggtgccg gaaaatccac ccttgtggac ctgctgcccc gtttttacga
      121 ttgcaccggg ggtgaactgc tggtggatgg ggtcaatgta aaggattatg taatatccga
      181 cctcaggcaa ctgatgggta ttgtaacgca ggaaaccata ttgttcaatg atactgtgtt
      241 caacaatatt gctttcggca tggaaagcgt gagcgaagaa gctgtgattc aggcggcaaa
      301 aattgccaat gcacacgatt ttatcatgca gctcgaaaac ggttatcaga gttatattgg
      361 cgacaggggt atgaagttgt cgggcggaca gaagcaacgg ctcagcattg ccagggccgt
      421 gctgctcaac cctcctgtta tgatactcga cgaagccacc tcgtcgctcg ataccgagtc
      481 ggaacgcctt gtgcaggaag ccctggtaaa acttatgcaa aaccgtacat ccgtggtaat
      541 cgcccaccgc ctttccactg ttcagtttgc cgacgagatt attgtcctgc aaaacggcaa
      601 gatagtagaa cggggcaccc ataccgaact ctccgccctt aaaggcgtct atcataaact
      661 ctgtgagatg cagactttta cctgatgccc ctcatgaatc ccatcgacga aagaattttc
      721 aaacttatca ataagcatca tatttttacg ctggcaaccg ctgaaaataa catgccttat
      781 tgctgtactt gtttttatgc tttcatgcgt gaaaaaaatc tttttgtttt tacttctgag
      841 tataaaacac aacatatttc tcattttcta aaacagcctg tagttgccgg atccatcgcc
      901 ctcgaaaccc gcatcatcgg gaaaataagg ggtttgcagt ttacaggaaa aatgattgag
      961 ttaaaagatc aggaattaaa ggaagcccga agggcatacc tcctgaaatt cccttatacc
     1021 aagcctttcc tgaaggatac gccgttttgg gggatagtcc ccgccttcta taaactcacc
     1081 gacaaccgac ttggctttgg tacaaaactc atatggagta atttagacaa tttataaact
     1141 ttttatcaac aaaatttgtt tggcacagcg ttaacaaata catctttttt tcgttaataa
     1201 gtatgcgatt ttgttaataa aaatgggttg ttttgcatgg ccgctttgta taatttagcc
     1261 atcccttttt gccttcggta aaaatgtaaa ttgtaattat tttaacaaat cattagtgtt
     1321 tttaaatata taaatattgt tatggaagaa aaatccccga cttttaaacc tcagccagca
     1381 gataacgagg aaaagattca ggaaacaaaa cgggaatcgg cgtatgataa attatatgaa
     1441 cacagaatac ctcccaaacc tgatgtctta tttattagtg aagaatcaaa tgaaataaag
     1501 atcgaaatgg aaaaaaaagg aaaaagcgaa caaacaacat actcgcacga gtcggccctg
     1561 gccgctacta ttgaatattt taaaggagat acccttgctg cagatgtgtg gataaataaa
     1621 tatgctctca aaaaccgcga cggcgaactg ctggaaaaaa ctccggacga tatgcaccgc
     1681 cgcatagcca gagaaatagc ccgcatcgaa aagaaatatc ctaacccgat gtccgaagaa
     1741 ctgatcttcg acctgctgaa agatttcagg tacattattc cccagggcag cccaatggca
     1801 gggataggta acgattttca aatatcctca ctttcaaatt gttttgttat tggtaacgac
     1861 actccctcag attcttacgg aggcatcatg aaaattgatc aggagcaggt gcagcttatg
     1921 aaaagacgcg gcggtgttgg ccacgacctg tcgcatatcc gcccccaggg cagcctggtg
     1981 aaaaacagcg ccctgacttc caccggcata gtgccgttta tggaacgtta ctccaattcc
     2041 acccgcgagg tggcccag
//
LOCUS       DAOSFP010000396         2055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_54345_length_2055_cov_3.060000, whole genome shotgun sequence.
ACCESSION   DAOSFP010000396 DAOSFP010000000
VERSION     DAOSFP010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54345_length_2055_cov_3.060000"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="PLI16_09895"
     CDS             <1..716
                     /locus_tag="PLI16_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84910.1"
                     /translation="TQKPLVMFNTASEPKTGIVAGEGIWRWRMQNYLQTGNQTVFNEL
                     VHKTIQYLSVKVDKDRFRVNVKNKFYENEAVEFDAEVYNDSYELVNDPEVTLTITNSA
                     NKTFPFTFSRTAHAYHINAGIFPLGEYRFTAQAKAGSTRLQKTGVFTVVPVNIESLNL
                     IADHQLMFSLAQKSNGKMLYPGQLSQIPGLLKARDDVKSVTYTKKRFSDVVNLFWVFL
                     IILALLSTEWFIRKRFGAY"
     gene            754..>2055
                     /locus_tag="PLI16_09900"
     CDS             754..>2055
                     /locus_tag="PLI16_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84911.1"
                     /translation="MKKIFSILVFTAWFVLNIHGQEQKPKLDESDKIGSSSKSAMFTA
                     ECMGLVNNPGTDLKWRPVLTQKPVSFRPETPNKEFIKKAKAEKMKQKALTVNNNDSNE
                     KSTLSVTPVVGTNYDANPNNGFSPLDNSMAISNGGWIVSVTNTTIRYDMMNGNNVYYS
                     SIVSFINDTAITHVCDPVVIYDAGSDRFIFFVQESSGLSTKSKLLIFFSKTNNPQNGW
                     WYYKLTGNPLNDESWFDYPKLAVSTNELYITGNLFYDSGGFNEAVLYQINKTSGYSGT
                     SMSWQYWHNISGSPFTLLPVSYGQGGSYGPGCYLVATDNNGSSNIKLYDLTDDMSSSN
                     EELNRYNISTPSYEFAGDSYQLNTACLLDNGDCRALSGFYLNGIIHFVFHSDIGSGWN
                     GINYNRLTVSTKTNESSLFGLVGTYDYSYPSVVSFASSTTDK"
BASE COUNT          621 a          423 c          430 g          581 t
ORIGIN      
        1 aaacacaaaa acccctggtg atgtttaata ccgcctcgga acctaaaacc ggaattgtgg
       61 ccggagaagg aatctggaga tggcgtatgc aaaactacct gcaaactgga aatcagacag
      121 tatttaatga gctggtacac aaaactatac aatacctgtc cgtaaaagtt gataaagacc
      181 gttttcgtgt aaacgtgaaa aataaattct acgagaatga agctgtagaa tttgatgcgg
      241 aagtgtataa cgatagttat gaactggtca acgatcctga agtaacgctc actattacca
      301 attcggcgaa taaaactttt ccttttactt tttcgcgcac agcccatgct tatcatatca
      361 atgccggtat tttcccgctt ggtgagtacc gttttactgc acaggccaaa gcaggatcta
      421 cccgtttaca gaaaacaggg gtgttcactg tggtgccggt aaatattgaa tcactcaacc
      481 tgatagccga ccatcagctg atgttttcac tggcccaaaa aagtaatgga aagatgcttt
      541 atccgggaca actttcacaa attcccggcc tgctgaaagc ccgtgatgat gttaagtcgg
      601 taacctatac aaaaaaacgt ttcagcgatg tggtaaacct tttttgggtg tttctgataa
      661 ttcttgcctt gctcagcacg gaatggttta tcaggaagag attcggggca tattagggtt
      721 gtgattttta tgcgaaataa aaaataataa actgtgaaaa agatattttc aattttagtt
      781 tttacagcat ggtttgttct aaacatacat ggacaggagc aaaaacccaa actcgacgag
      841 agcgataaaa ttggcagttc atcaaaatca gcaatgttta ccgccgaatg tatggggctg
      901 gtcaacaatc cgggcacgga ccttaagtgg aggccggtcc tgacacaaaa gccggtttct
      961 ttcaggcctg aaactcctaa taaagaattc attaaaaagg ccaaagctga aaaaatgaag
     1021 caaaaagcac taactgtaaa taacaatgac tcaaatgaga aatcaacgtt gagcgttacc
     1081 ccggtggttg gtacaaatta cgatgccaat cctaataacg ggttttcccc tctggataat
     1141 tccatggcga tatccaacgg cggctggata gtcagcgtta caaataccac cataaggtat
     1201 gacatgatga atggaaataa tgtgtattac agcagcatag tttcattcat caatgacact
     1261 gctattaccc acgtttgcga tccggtagtg atttatgatg ccggttccga caggttcata
     1321 ttttttgtgc aggaatcatc cggtctttca acaaagtcga aattattgat ctttttctca
     1381 aagactaata atcctcaaaa cggatggtgg tattataaac ttaccggcaa tcccctgaat
     1441 gatgaaagtt ggttcgacta tcccaaatta gcggtttcaa ccaatgaatt gtacattacc
     1501 ggtaacttgt tttatgattc aggtggcttc aacgaagctg ttttatacca gataaacaaa
     1561 accagcggat actccggcac ttctatgtcg tggcagtatt ggcacaatat ttctggttct
     1621 ccttttacat tattgcccgt ttcttacgga cagggaggca gttacggacc gggctgttac
     1681 ctggtggcta ccgacaataa cggaagttca aatattaaac tgtatgatct gaccgatgat
     1741 atgagcagta gtaacgagga gcttaaccgg tacaatattt ctactccttc ctatgaattt
     1801 gccggagatt cttaccaact gaatactgcc tgcctgctcg acaatggcga ttgccgtgcc
     1861 ttaagcgggt tttacctgaa cggtatcatc cattttgttt tccattctga tatcggcagc
     1921 ggctggaacg ggataaatta taaccggctt actgttagta caaaaaccaa tgaatcttcc
     1981 ttgttcggcc tcgtaggtac ttatgattat tcttatccat ctgttgtttc ttttgcgagc
     2041 agtacaaccg ataaa
//
LOCUS       DAOSFP010000397         2048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_54612_length_2048_cov_5.931761, whole genome shotgun sequence.
ACCESSION   DAOSFP010000397 DAOSFP010000000
VERSION     DAOSFP010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54612_length_2048_cov_5.931761"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            673..1314
                     /locus_tag="PLI16_09905"
     CDS             673..1314
                     /locus_tag="PLI16_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84912.1"
                     /translation="MKNTLALLLFAFAPFAVFSEINTGNSLEWLCADAGLIARGHIIS
                     YSKTNTEKNNFIIVFETKEIFKGTTESPVEIVTATIAEDSLKKYTEEKTPLLVFLQEG
                     EKSFRDKKKKTPWHIMEACNAVSALINLAAPQQVLIKASTFTILGSREQIIAECRLSL
                     KKIAGYEVQGKTVFMNYLTVPFQTQAYGLLYSGSTCYLAVPDFMFPDSREKPY"
     gene            1323..>2048
                     /locus_tag="PLI16_09910"
     CDS             1323..>2048
                     /locus_tag="PLI16_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HOH84913.1"
                     /translation="MKPAFQITTPVQLRDVLFGFRVSRVILTAFELDVFTHIATGGSV
                     SEMVAAKIKADHRATDRLMNALCAIGLLKKRKGLFFNTTFSAQFLSKNSPGYLYGLMH
                     TANMWDTWSTMTEMVRTGKTQRDKVESRKSNLFSEAFIGAMHERAAQQAKGVLDKLNL
                     KGVRHFLDIGGGSGVYAMDFVKRDAENRATVFDLPDIIPITKKYVKKEGLLKRFSFIG
                     GDYNKDSLGAGYDLAFLSAIVHIN"
BASE COUNT          601 a          439 c          452 g          556 t
ORIGIN      
        1 acctgaattt ttgtcattaa atgttatttt tgtgcgcagc ataattacca ctctgtgtga
       61 cttaaaaaat catgcaaaac tcaaatcaat aacatatgat ttgattcgtt aatgcgtaat
      121 cgacaaagaa aatgtaatac cgccatagcg gaaacatttt ctaagtcggc ctgcctgccg
      181 caggcagggg ttaccccccg caacttcgtt cacggggcgc aatagttcac tacgttccta
      241 ttgctcaatt ccatctaaca taataataag attaactgga tttcaataaa ttcaaaaatt
      301 aatgaattat cagcacctta tcaaacattt tattttttcg tagaaaaaaa gtgcgttggc
      361 ctgcccagac ggcgggccag ctcaggcgtg aatcagaaaa tatttgaaaa tcttcaaagg
      421 ccccgcagat tgcggggttt cccgacgcat ggcgtcgggc ctactttgat tagattttca
      481 atattttctg agagcgggct gaagcattgt ctgggctgtc tttttctttg gttcatttct
      541 tttggacaag caaaagaaat gaacatacaa atccaaaaca aaatcatttt aaagttaagt
      601 gttttattct atttcttcaa aatcttcgaa tctgggatta tatttatctt tatagccata
      661 aaacgcgtgg tgatgaaaaa taccttagca ttattgctgt ttgcttttgc gcctttcgcg
      721 gtattttccg aaataaatac cggcaacagc ctggaatggc tttgtgcgga tgccggactg
      781 atagcccgcg gccacataat cagttattca aaaacaaata cggaaaaaaa taattttatc
      841 atagtttttg aaacgaaaga gatatttaaa ggtaccaccg aaagcccggt ggagattgtt
      901 acagctacta tcgctgaaga cagcctgaag aaatacacgg aagaaaaaac cccgctgctg
      961 gtttttttac aggaaggaga gaaatccttc agggataaaa agaaaaaaac gccgtggcat
     1021 atcatggagg cctgtaatgc cgtttcggca ctgataaacc ttgcagcgcc gcaacaagtg
     1081 cttattaaag caagcacgtt tacgattttg ggaagccgcg aacagatcat tgccgaatgc
     1141 cggctaagcc tgaaaaagat tgccggatat gaagttcagg gtaaaacagt gtttatgaac
     1201 tacctgacgg ttccgtttca gacacaggct tacggcctcc tgtattcagg cagtacatgt
     1261 tatcttgccg ttcccgattt tatgtttccc gacagcaggg aaaaacctta ttaaacaaaa
     1321 ttatgaaacc tgcttttcaa attaccactc ccgtgcaact gcgcgatgtg ctttttggct
     1381 ttcgtgtcag ccgcgttatt cttaccgctt ttgagctgga tgtgtttacc catattgcta
     1441 ccggaggttc cgtttccgaa atggttgctg caaaaataaa agcagatcac agggccaccg
     1501 accgcctgat gaatgccctg tgtgccatag gtttgctaaa aaaacgaaaa ggattgtttt
     1561 tcaatacaac attttcggcg caatttcttt cgaaaaacag tcccggttac ctgtatggcc
     1621 tgatgcatac agccaatatg tgggacacct ggtccaccat gaccgagatg gtaagaaccg
     1681 ggaaaaccca gcgcgacaag gtggaaagcc gcaaaagcaa cctcttttca gaagccttta
     1741 tcggggcgat gcacgagagg gcggcgcagc aggccaaagg cgtgctggac aaattaaacc
     1801 tgaaaggagt caggcatttc ctggatatcg gcggaggttc cggcgtgtat gccatggact
     1861 ttgtgaagcg ggatgccgaa aaccgggcca cggtgttcga tctgcccgac attatcccga
     1921 ttacaaaaaa atatgtaaaa aaggaaggct tgctgaaacg cttcagcttt attggcggtg
     1981 actataataa agattctttg ggggccggtt atgacctggc ttttttatcg gccattgtcc
     2041 acatcaac
//
LOCUS       DAOSFP010000398         2041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_54908_length_2041_cov_3.065962, whole genome shotgun sequence.
ACCESSION   DAOSFP010000398 DAOSFP010000000
VERSION     DAOSFP010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54908_length_2041_cov_3.065962"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1109
                     /locus_tag="PLI16_09915"
     CDS             192..1109
                     /locus_tag="PLI16_09915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84914.1"
                     /translation="MNKKTLVYGFALLFAVLTSKAQNTDALFISGFNNLQNNKINEAI
                     DDYTKVIALKPDYTDAYFNRAIAYVRLEKYKEAINDYTQVLLLKPDDENALLNRGMLY
                     IDQKKNANALTDFNKALEINPGFSNVYYQRGLLYRQAGKDTAAMNDFNKFISLAPQSV
                     AGYEALGGLYDQMEEYDAAIQTYSKILSIDATNARAYFNRAYDYDLVTKTENALTDYS
                     QAIQYNEKYSDAFFNRAYIYNDLNEFDKAIADYDKVIEYEPDSPDAYFNRGIAKINAG
                     RKHDGCSDIQKAASLGDKTAKKYCAVNCK"
     gene            1109..1342
                     /locus_tag="PLI16_09920"
     CDS             1109..1342
                     /locus_tag="PLI16_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOH84915.1"
                     /translation="MRKKISGLFLTVFNFYYDGFRSMTMGKKLWTIIIIKLFIMFVVL
                     KLFFFPDFLQTKFENDKQRSDYVLEQLTMPTNK"
     gene            1349..>2041
                     /locus_tag="PLI16_09925"
     CDS             1349..>2041
                     /locus_tag="PLI16_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOH84916.1"
                     /translation="MISDIDITLVDWSRAQFALTAIYHWFFVPLTLGLSFMIAIMETI
                     YVKTGNEEWKRITRFWMKLFGVNFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGILAFFLESTFIAVMFFGWNKVSKKFHLASSWLVAFGSNLSALWILVANSWMQYPV
                     GMEFNPETVRNEMISFWDVLLSPVAVNKFLHTISSSYVVASLFIVGVSAWYLLKKREL
                     LLARRSIVLASVF"
BASE COUNT          622 a          404 c          424 g          591 t
ORIGIN      
        1 ataccaaata atcaagatat tatgaagaaa gtgttctaat gctacgcgtt agaaaatctt
       61 taatgcgtaa tttgggataa aaccatcctt ccggagcaaa gaacaggcta tatagggcaa
      121 ttatcaaatt atttaggcat ttatatactc aatacggaat aattattatt tttgtacaaa
      181 taatgataat tatgaataag aaaactttgg tatatggctt tgctctgctc tttgctgtac
      241 ttacctccaa ggcgcagaac accgatgccc tgtttatcag tgggtttaac aacctgcaga
      301 acaataaaat aaacgaggcc attgacgatt acaccaaagt tattgccctg aaacccgatt
      361 ataccgatgc ttatttcaac cgcgccatcg cttatgtaag actcgaaaaa tacaaagaag
      421 ccatcaatga ttatacacag gttcttttat taaaacctga tgatgaaaat gcattgctca
      481 accggggtat gctgtatatc gaccagaaaa aaaatgcaaa cgcactgact gatttcaaca
      541 aagcgcttga aattaatccg ggtttttcta atgtctatta tcagcgaggc cttctttacc
      601 ggcaagcggg gaaagacact gccgccatga atgatttcaa taaatttatt tctctggcgc
      661 cgcaatccgt tgccggatat gaagctctcg gcgggctgta tgaccaaatg gaagaatacg
      721 atgcagccat acagacgtac tcaaaaatcc tcagtataga tgctacaaat gccagggcat
      781 attttaacag ggcatacgat tatgacctgg taactaaaac agaaaatgcc cttacagact
      841 actcgcaggc tatacagtat aatgaaaagt atagtgatgc attttttaac cgtgcctata
      901 tttataacga cctcaacgaa ttcgacaaag ccattgcgga ttatgacaag gtgattgagt
      961 atgagcccga cagtcccgac gcttatttta acagaggaat tgcaaaaata aatgccggac
     1021 gaaaacacga cggttgctcc gatatacaaa aagcagcttc actgggcgat aaaactgcca
     1081 aaaaatattg tgctgtaaac tgcaaataat gcgaaaaaaa atctcaggcc tatttttgac
     1141 agtcttcaat ttttattatg atggcttcag aagtatgacc atgggaaaaa aattatggac
     1201 aataattata attaaacttt tcatcatgtt tgtggtgctt aaactttttt tcttccctga
     1261 ttttctacag acaaagtttg aaaatgataa acaaagaagc gattatgtgc tggaacaatt
     1321 gacaatgccg acaaataaat agttgttcat gatttcagat atcgatataa ccctggttga
     1381 ctggtcgcgc gcacaatttg cgctgacagc aatataccat tggttttttg tgccgctcac
     1441 ccttgggctg tcgtttatga tagccatcat ggaaacgatt tatgtgaaga cgggtaatga
     1501 agaatggaaa aggataacca ggttctggat gaaattattc ggagtaaatt ttgctatcgg
     1561 tgtggccacg ggcatcatcc ttgaatttga gtttggcacc aactggtcga attattcatg
     1621 gtttgtcggc gacatttttg gtgcaccgct tgccatcgaa ggcatattgg ctttttttct
     1681 ggaatccaca ttcattgctg tgatgttctt cggatggaat aaggtgagca aaaaatttca
     1741 cctggcttcc tcctggctgg tagcctttgg atcgaacctt tcggccttat ggatattggt
     1801 tgccaactca tggatgcaat atcccgtcgg catggagttc aaccccgaaa cagtccgcaa
     1861 cgaaatgata agtttttggg atgtgctgct atctccggtg gccgtcaaca agtttctgca
     1921 cactatcagc tcttcctatg tggtagcgtc tttattcatt gtgggggtca gtgcgtggta
     1981 cctgctgaaa aaacgcgagc ttttactggc ccggcgtagc atagtgcttg cctcggtatt
     2041 c
//
LOCUS       DAOSFP010000399         2015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_56009_length_2015_cov_3.303571, whole genome shotgun sequence.
ACCESSION   DAOSFP010000399 DAOSFP010000000
VERSION     DAOSFP010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56009_length_2015_cov_3.303571"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1099)
                     /locus_tag="PLI16_09930"
     CDS             complement(128..1099)
                     /locus_tag="PLI16_09930"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOH84917.1"
                     /translation="MKKTVFFLCFAAILFASCGPGKQKDDKTTADADRAGVSGMINVD
                     GDGILYPLMNKWKQEFAKEFSEVKIGIKTGNTGSGLKRLAAGELQMVMAARELSETEK
                     QSGFWAVPVAKDVVLPVISFDNSNLQKIVFTGVTKEKMASAFTGKISSWGQLLQQPSK
                     DPVELYAMNDSADISLFWAGFLGVDAGNIKGNKVSGENEMAMKVAANKNALGYCSMTY
                     VFDITTGFKKKNLYVLPIDLNANKMADDNELVFDKLDDIKSAVSSGKYPSPPSRKLYL
                     VCKTKPSDKATVSFIKWILTVGQNHCGPFGFINLSTEEAAGYLKQLE"
     gene            1283..>2015
                     /locus_tag="PLI16_09935"
     CDS             1283..>2015
                     /locus_tag="PLI16_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84918.1"
                     /translation="MNIYIKKRRWKILLFVTLLIIFSLSLWYIDRVANQIETDQRNKI
                     KLWVDAITKKASLVNATDTFFTLLQSEERKKVELWANANVRLINATYEEDLTFYLDII
                     AGNNTIPVILTDENNKITLAKNVDFNLDSVKTLSGKIKEDFSKYPPIEINYIGKKKLL
                     LHYRDSKVFTGLREVLDKQVKAFLSEIVTNAPSVPVIVMDADRKQVIAYGNIDTVKLQ
                     DQNYVTQIIQSMEADNNTIAVNLPGK"
BASE COUNT          557 a          472 c          423 g          563 t
ORIGIN      
        1 gattataaat ctgattaata agaataataa gacacgctaa cacataattt ttctatgtcg
       61 ggattacccc ccgcaacttc atgcacgggt cacaatagtc cactacgtcg cgattgcgca
      121 atccgggtta ttccaactgt ttgagatatc cggcggcttc ttctgtgctg aggtttatga
      181 aaccaaatgg tccgcagtgg ttctgcccca cggtcagtat ccatttgata aaagacaccg
      241 tggccttatc cgagggtttg gttttgcata ccaggtaaag ttttcgcgac ggcggcgaag
      301 gatattttcc cgaagaaaca gcacttttta tatcgtccag tttatcgaaa accagctcgt
      361 tatcgtcggc cattttgttg gcgttcaggt cgatgggcag cacataaagg tttttctttt
      421 tgaaaccggt ggtaatatca aacacatagg tcatactgca atagcctaac gcgtttttgt
      481 tggcggccac tttcatggcc atttcatttt cgcccgacac cttgtttccc ttgatattgc
      541 cggcatccac tcccaggaaa cccgcccaga acagggaaat atcagcgctg tcgttcatag
      601 catacaactc cacaggatct ttggaaggtt gttgcagcaa ctggccccag gaagatattt
      661 ttccggtaaa ggcgctggcc atcttttctt tggtaacgcc tgtaaaaaca attttctgca
      721 aattactgtt atcaaagctt atcaccggca acaccacatc tttagccacc ggcacggccc
      781 agaagccgct ttgtttttcg gtttcgctca gttcgcgggc ggccataacc atctgtaact
      841 ctccggcggc aagtcttttt aagccgctgc ccgtgttgcc ggtttttata ccaatcttaa
      901 cttcgctgaa ttctttggca aattcctgtt tccatttgtt catcagggga taaaggatgc
      961 cgtccccgtc aacgttaatc attccgctca cgcctgcacg atccgcatcg gcagttgtct
     1021 tgtcatcttt ttgtttaccc ggtccgcaag acgcaaaaag tatggctgcg aagcataaaa
     1081 aaaacacggt ttttttcatt tgagaatttt tcttcaaaaa tacatctttt aggcttttgt
     1141 tttactgcgt gcgacagtat gcttttttat aaggtttatg tatatttgca ataatttacg
     1201 catataacac ggccagggcc ggcaataaat agtggcaaat gaacgtttca acacctgctg
     1261 aacaggaaaa aaaacagtgc tgatgaacat ttacatcaaa aaaagaaggt ggaagatttt
     1321 gctttttgtt accctgttga taattttctc tttatcattg tggtacatcg accgcgtggc
     1381 caaccagatt gaaacggacc agcgaaacaa aataaaactc tgggtggatg ccatcaccaa
     1441 aaaagccagc ctggtaaatg ctacggatac ttttttcacg cttttgcagt cggaagaacg
     1501 aaaaaaagtg gaattatggg ccaacgccaa cgtgcgtctc atcaatgcca cttacgaaga
     1561 agacctcacc ttttacctgg acatcatcgc gggcaacaac accatacctg tgatactcac
     1621 ggatgagaac aacaaaatta ctttagcaaa aaatgtggat tttaatcttg attccgtgaa
     1681 gaccttgtcg ggaaagataa aagaagattt ttcaaaatat ccccccatcg agatcaacta
     1741 tatcggcaag aagaaacttc tgctgcatta cagggactcc aaggttttta caggattgcg
     1801 tgaagtgttg gacaagcagg taaaagcctt tctttcagag attgttacca atgccccctc
     1861 ggttcccgtt attgtaatgg acgccgaccg caaacaggtg attgcctatg gcaatatcga
     1921 cacggtgaaa ctgcaggatc agaattacgt tacccaaatt atacagtcga tggaggccga
     1981 caacaacacc attgcggtaa acctgcccgg taaag
//
LOCUS       DAOSFP010000400         2014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_56034_length_2014_cov_4.276672, whole genome shotgun sequence.
ACCESSION   DAOSFP010000400 DAOSFP010000000
VERSION     DAOSFP010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56034_length_2014_cov_4.276672"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="PLI16_09940"
     CDS             <1..1393
                     /locus_tag="PLI16_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOH84919.1"
                     /translation="DEPTNHLYIESIQWMEDFLQSYKGAVIVVSHDRAFLDNVTRRTV
                     EISLGKIYDYKASYSDYVEMREERIESQLATYNNQQKQIEQIERFITRFRYKATKARQ
                     VQSRVKMLEKMEMVEIDETDASAIRFLFPPAPPSGRVSVQADKVSKSYGEKLILKDVD
                     LLVPRGEKIAFVGRNGEGKTTLVKMLLNQIEYTGKILLGHNIIPGYYAQNQTEMLDGE
                     KTVFQTIDEVAVGDIRPKIRALLGSFLFGGDTIDKKVKVLSGGEKSRLALAKMLLSPV
                     NLLVLDEPTNHLDMVSKDILKNALIRFDGTLIIVSHDRDFLQGLTNKVYEFKNKTIKE
                     YIGDIYYFLDAKKISSLKELERNVQGKVPAANKPAISDNKLNREKKKDFEREQRKLVN
                     KITLAEQHIEKLEAEIAGLDKVLTDPEKYKEALNDKEIFDNYQKFKSQLKDEIDKWEE
                     LNRELDELNAKNT"
     gene            complement(1405..1926)
                     /locus_tag="PLI16_09945"
     CDS             complement(1405..1926)
                     /locus_tag="PLI16_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84920.1"
                     /translation="MTLLLSLLQVDTFFKVIQIVVLSIAKLIFAPPLSLEMGLGYFET
                     VAITAISGIIGIFLFYHLSGLIIRFYKRKIRPYFRRIFATKNTAASAGNNTSGLATSD
                     KNSKWQMKTYVKKVRNKWGLYGICMFTLIAIPLVSFFANKYYHKNKYTLVYLSASVVI
                     WSFVVTTVYFFAF"
BASE COUNT          660 a          386 c          454 g          514 t
ORIGIN      
        1 tgacgaacct accaaccatc tgtatatcga atccatacag tggatggagg attttttgca
       61 atcttacaaa ggagccgtca tcgtggtgtc tcacgacagg gcttttcttg ataatgttac
      121 ccggagaacc gtcgaaattt ctttgggtaa aatttatgac tacaaagctt cgtattctga
      181 ttatgtggag atgcgcgaag aacgcattga aagccagtta gccacctata ataatcagca
      241 aaaacagatt gagcagattg agcggtttat cacacgtttc cgttacaagg ccaccaaggc
      301 caggcaggtg cagtcgagag tgaaaatgct cgaaaaaatg gagatggtgg aaattgatga
      361 aacagatgct tcggccatca gatttttgtt tcctccggcg ccaccttcag gtagagtaag
      421 cgtgcaggcc gacaaagtgt cgaaaagtta cggtgagaaa ctaattttga aggatgttga
      481 cctcctggtg ccacggggtg agaaaatagc gtttgtagga cgcaacggcg aaggaaaaac
      541 caccctggta aagatgttgc tgaaccaaat tgaatataca ggcaagatac ttcttggtca
      601 caatattatc cccgggtatt atgcccagaa tcaaaccgaa atgctggatg gtgaaaaaac
      661 agtttttcag accatcgacg aagtggctgt gggcgatatc aggcctaaaa tcagggcctt
      721 gctcggcagt tttctttttg gaggcgatac tatcgacaaa aaggtaaaag tgctgtcggg
      781 cggtgaaaaa tcacggctgg ctctggctaa gatgttgttg tcgcctgtta atttgctggt
      841 gctcgacgag cctaccaacc atctcgatat ggtttcaaaa gacattttga aaaacgcgtt
      901 gatccgtttc gacggcaccc tgattattgt ttcgcacgac cgtgattttc tgcaaggcct
      961 taccaacaag gtctatgaat tcaaaaacaa aaccataaaa gagtacatcg gcgatattta
     1021 ctattttctg gatgccaaaa agatctcttc actcaaagaa ctggagcgaa atgtgcaggg
     1081 aaaagtgccg gccgccaata aaccggccat ttcggataat aagctgaacc gcgaaaagaa
     1141 aaaggatttt gaacgcgaac aacgcaagct ggttaacaaa attaccctgg ctgaacagca
     1201 tatcgaaaaa ctcgaagcag aaattgccgg attggataag gtgcttacag accccgaaaa
     1261 atataaagaa gccctgaatg ataaagaaat atttgataat tatcaaaaat tcaaaagtca
     1321 gctgaaagac gaaatagata agtgggagga gttaaacagg gaactggatg aactcaacgc
     1381 caaaaatacc tgaagggcct gattttaaaa ggcaaagaaa tataccgtag taacaacaaa
     1441 agaccagatt accactgaag cgctgagata taccagcgta tatttgtttt tgtggtaata
     1501 cttgttggca aaaaatgaaa ccagaggtat cgcaatcagt gtaaacatac agatgccgta
     1561 aagcccccat ttattacgta cttttttcac gtaagttttc atttgccatt ttgaattttt
     1621 atcgctggtg gcgagtcctg acgtattatt tcctgctgag gctgccgtgt tttttgtggc
     1681 gaaaatccgg cggaaataag gccttatttt tcttttgtaa aatctgatga taaggccact
     1741 taaatggtaa aacaggaata tccctatgat gccgctgata gcagtaatag ctaccgtttc
     1801 aaagtaaccc aatcccattt cgagcgacaa gggaggtgca aagatcagct ttgcaatgct
     1861 cagcaccact atttgtatta ccttgaaaaa tgtatcgact tgtagcagtg agaggagaag
     1921 agtcatttta atgataaaaa actataattt caagacaaaa gtatgaaaac atctgaagat
     1981 aaacaagaga aatattaaat ttttactcca tagg
//
LOCUS       DAOSFP010000401         2013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_56067_length_2013_cov_4.641471, whole genome shotgun sequence.
ACCESSION   DAOSFP010000401 DAOSFP010000000
VERSION     DAOSFP010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56067_length_2013_cov_4.641471"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PLI16_09950"
     CDS             <1..706
                     /locus_tag="PLI16_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH84921.1"
                     /translation="LQSGLRSMTISLDGFEDSHNWMRGNKHSFEKAVNAIKLAVSAGD
                     SIVFDVVTCVNQKNFDELPELKKFLIGLGVKRWRIFSICPIGRAKENPLLSISNEQFR
                     RVLDFIKANREEGLIQTSFGCEGFLGDYEAEVRDGFFFCRAGVNIASVLADGSVCACP
                     NINRDAFTQGNIYKDDFIDVWNKKFQPMRDRRWTQEGLCADCKEWKWCRGNGIHLRDV
                     KNHDVLRCHYKMIEEL"
     gene            complement(815..1462)
                     /locus_tag="PLI16_09955"
     CDS             complement(815..1462)
                     /locus_tag="PLI16_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84922.1"
                     /translation="MILIVAIVVLFISGLLYLIFIGNSNSANREINSPDEDIAKNDGT
                     FKYPCPMESDPGWGEAMFPWHIVDGIRVRGSFWAYGLAFTGGRENYEDSCGWRQATID
                     FGESKQFNRVVIWHGGNNVDIPVYYLRCWNDSKENWDTLICKRDMASRATEYQKDHWL
                     SIPVEDTFSTVGSRKIQYLFNNCNGAHGWINEFEVYNDAKGDRPECLIGPLGIRK"
     gene            1589..>2013
                     /locus_tag="PLI16_09960"
     CDS             1589..>2013
                     /locus_tag="PLI16_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84923.1"
                     /translation="MFEHMRAFIFSITCFIILLCVCGYSHSQSKSKADQLAEELFHHP
                     DDLKDDTATVWKFYEYAILCSEKEGAWNSDKEPNVCMYGFHMSERLGYTRGIREFTTV
                     LQKCFKQTEGLPEIIVHTYPDAVCRDTLRLLYQARKQRHG"
BASE COUNT          573 a          456 c          434 g          550 t
ORIGIN      
        1 attacaatcc ggcttgcgtt ccatgactat cagccttgat ggctttgaag acagccacaa
       61 ctggatgcgc ggcaacaaac atagttttga aaaggccgtg aatgccatta aacttgccgt
      121 aagcgccggc gacagtattg ttttcgatgt ggttacctgt gtcaatcaaa aaaattttga
      181 tgaattgccc gaacttaaaa aattccttat cgggcttggc gtaaaacgtt ggcgtatttt
      241 cagtatctgc cctatcggac gggccaaaga gaatccttta ctaagcatca gcaacgaaca
      301 gtttcgccgt gtacttgatt ttatcaaagc caaccgggaa gaaggcctga tacagaccag
      361 ctttggctgc gaaggttttc tgggtgatta cgaagctgag gtgcgcgatg gatttttctt
      421 ttgccgggcc ggagtcaata tagcttcggt actggccgac ggttctgtct gcgcctgccc
      481 gaacatcaac agggatgcat ttacgcaggg caacatttat aaagatgatt tcatcgatgt
      541 ctggaataaa aaatttcagc ccatgcgcga ccgccgctgg actcaggaag gtctgtgcgc
      601 tgattgcaaa gaatggaaat ggtgcagggg caacggcata catctgcgcg atgtgaaaaa
      661 ccatgatgtg ctgcgctgcc attacaagat gatcgaagaa ttgtgaatgc agcaactaca
      721 gcatgttaaa atcaaaaaaa tgtaaaggcc ggaaaaatgt tgtaatggtt tcagtcactg
      781 cccctgctga taaacattaa atcatcagca aaaattattt cctgatgccc aaaggaccaa
      841 tcaggcattc aggcctgtcg ccctttgcat cgttgtagac ctcaaactca ttgatccagc
      901 cgtgggcccc gttgcaatta ttgaacaggt actgaatttt acgggaaccc accgttgaaa
      961 aggtatcttc taccggaatg ctcagccaat gatccttttg gtattcagta gcccttgacg
     1021 ccatatccct tttacaaatc agagtgtccc aattttcttt actatcattc cagcaacgaa
     1081 gataataaac agggatatcg acattattgc cgccatgcca gattaccacg cggttgaatt
     1141 gtttggactc gccaaaatca atggtagcct gacgccagcc acaggaatct tcataatttt
     1201 cccgccctcc tgtaaaagcg aggccataag cccagaaact gcctcgcacc ctgatgccat
     1261 ccactatatg ccatggaaac atggcttcac cccagcccgg gtctgactcc atcgggcagg
     1321 gatatttaaa cgtgccgtcg ttctttgcaa tatcttcatc cggactattg atttctctgt
     1381 tcgcggaatt actgtttcct ataaatatca ggtacaacaa gccggagatg aatagcacaa
     1441 caatcgctac gatcaggatc aaataaactt tttttcggct ttccgcaaca ggcataatct
     1501 taagtcttta taaacagctg taaatgtttc tctcttaaag tattttttaa aaagaatatt
     1561 ctggttcaaa aataattata tttgccttat gtttgaacat atgcgtgcat ttatattttc
     1621 aattacctgc tttatcattt tgctatgtgt ctgcggttat tcccacagcc agtccaaatc
     1681 aaaggcggac caacttgcag aggagctgtt ccaccatccg gacgacttaa aagatgatac
     1741 agccaccgtc tggaaattct atgaatatgc catattatgt tctgagaaag aaggtgcctg
     1801 gaattcagat aaagagccta atgtatgcat gtatggattt catatgagcg agaggcttgg
     1861 ttatacaaga ggtatcaggg aatttactac tgtattacaa aaatgcttca aacagacaga
     1921 aggactgccg gagatcattg ttcacacata tcctgatgct gtttgccgcg acacgctgcg
     1981 tttgctttat caggcgcgaa aacaacgtca cgg
//
LOCUS       DAOSFP010000402         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_56944_length_1993_cov_3.383901, whole genome shotgun sequence.
ACCESSION   DAOSFP010000402 DAOSFP010000000
VERSION     DAOSFP010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56944_length_1993_cov_3.383901"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PLI16_09965"
     CDS             complement(<1..739)
                     /locus_tag="PLI16_09965"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOH84924.1"
                     /translation="MAGYKFTVKIAGIINDTCYLGHYYGQHQYIDDTAMTNAKGEMVF
                     TGKEALPGGIYFVVYPKKKYFEFIVNKEQTISLETDTVYPIKNMKIKGSEENQIFYKY
                     LNVIDENQKKYDPLKKRMEANKNNKDSTEAIRKEMEKIDDFVKNYKLELIKSHPETLI
                     ANVFVSSRDPEVPEAPVLANGRKDSTFRYYYFKNHYWDNITFSDDKILRTPVFYNRIS
                     NFFTNVVVQQPDSIILEADKLVEKARAN"
     gene            1012..1842
                     /locus_tag="PLI16_09970"
     CDS             1012..1842
                     /locus_tag="PLI16_09970"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOH84925.1"
                     /translation="MKKAALLIIAFCALSLAGFGQHFNAGAKAGLNMIPLEKNDLKGR
                     LFSAGYHVGAVGSYKVNDWFSVSAEIMLTSQKKTYEKLDTASFLGTLAENPLLSFTGI
                     DIDEIMDTLGMIKDYINDNVYNTRTGVVNLTYIKVPVLACFNYKSLYFSVGPYVSFLL
                     SNKGNEELSQLIPIVETMTGLDTIPFYYSIINAMFPGYSEPLASKVEKDKTIGKIDFG
                     VMAEVSYRLGNNVTLGVSYHQGLLNYRKPEIYKNDYLSSLNFSIGYLFNFPANNKSIY
                     "
BASE COUNT          565 a          386 c          400 g          642 t
ORIGIN      
        1 gattggcccg ggctttttcc accagtttgt cggcttccaa aatgatggaa tcgggttgtt
       61 gaacaaccac attggtaaaa aaattactga tacggttgta aaatacaggt gtcctaagaa
      121 ttttgtcgtc cgagaaagtg atgttatccc aataatggtt tttaaaatag taataccgga
      181 aagtggaatc tttgcgtccg ttggccaaca ccggtgcttc gggtacttcg ggatcgcgtg
      241 acgaaacaaa cacattggca atcaatgttt cgggatggct ttttataagc tcgagtttat
      301 aattcttaac aaaatcatca atcttttcca tttccttacg gatggcttcg gtgctgtctt
      361 tattattttt attggcctcc attcgttttt tgagcgggtc gtattttttt tgattttcat
      421 caatcacgtt caaatactta taaaaaatct ggttttcctc cgacccttta atcttcatgt
      481 tttttatcgg gtacacggtg tctgtctcca gcgaaatagt ctgttctttg ttaacaataa
      541 attcaaaata tttctttttc ggatacacta caaaataaat tcccccgggc aatgcttcct
      601 tgccggtaaa aaccatctcg cctttggcat tggtcatggc cgtgtcgtcg atatactgat
      661 gctgcccgta ataatgtccc aggtaacatg tatcgttaat aatgcctgca attttgacag
      721 tgaatttgta ccctgccatt tcagttgcag tatgggatac tggtttttcc gcctggtaat
      781 taactataaa taatgcagtt aggataatat ttgagatata actaaacata aggtaaaata
      841 ttttgctttg gacttgcaaa tttaatgcta aagaagatat ttagagcggt ttttaataat
      901 ttttccatgt aaatgaatac aacgtttatg aaaatttggg ctgcaccgga aacaccaatt
      961 gaatatttgt agtcaaatat attaactttg ccgggctaaa aacatagaaa catgaaaaaa
     1021 gcagcattac tgataatagc attttgtgcg ctttctctgg ccggattcgg acagcatttt
     1081 aatgcaggag ccaaagcagg cctcaacatg ataccgcttg aaaaaaacga tctgaaaggc
     1141 cgcttattca gtgccggtta ccatgttggc gcagtgggca gttataaggt taacgactgg
     1201 ttttcggttt cggcagaaat tatgctcacc tcgcaaaaga aaacttatga aaagctggat
     1261 acggcttcat tcttaggcac actggctgaa aatccgttac tttcgttcac cggcattgat
     1321 attgatgaaa taatggatac gctgggaatg attaaggatt atatcaacga taatgtgtat
     1381 aacacccgta caggcgtggt aaatctaact tatatcaaag tacctgtgct agcttgtttc
     1441 aactacaaat cgctgtattt ctcggtaggg ccttatgttt cttttttgtt atcaaacaaa
     1501 ggcaacgaag agctcagcca gcttattccc attgtggaaa cgatgacagg cttggacacc
     1561 atcccatttt attattccat catcaatgct atgtttccgg gctacagcga acccctggcc
     1621 agcaaggtgg aaaaagacaa aactatcggg aaaatcgatt ttggcgttat ggccgaggtt
     1681 tcataccgtt tgggcaacaa tgtaaccctg ggcgtgagtt atcatcaggg attgctcaat
     1741 taccgcaagc cggagattta caaaaatgat tacctgtcgt cgctcaattt ttctatcgga
     1801 tatttgttca attttccagc aaacaataaa tccatttatt aatcagaggc tgtctcaaaa
     1861 tgtatttttt ttgatattct tctctcagtg gctctctgtg aaccgcaata gcggtctctg
     1921 tgaatatctg tgtaataagt aagcgaccgg caaagtgcat ctttcttata aggccgtagt
     1981 gatctatttt tgc
//
LOCUS       DAOSFP010000403         1984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_57340_length_1984_cov_3.910835, whole genome shotgun sequence.
ACCESSION   DAOSFP010000403 DAOSFP010000000
VERSION     DAOSFP010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57340_length_1984_cov_3.910835"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLI16_09975"
     CDS             complement(<1..1241)
                     /locus_tag="PLI16_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84926.1"
                     /translation="MSDKENEVVGTNGNGNGNGNYDADSIKVLEGLEAVRKRPSMYIG
                     DTGSRGLHHLVVEVVDNSIDEALAGYCDRIDVTINTDNSITVEDNGRGIPTGMHEQEH
                     RSALEVVMTVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSDMKVTVFREGKIFEQEY
                     KCGDPQYAVREIGESEKTGTTITFKPDKAIFYVTEYSYELLAARLRELAYLNKKVRLT
                     LTDMREKDENGQSINEEFYSEHGLRDFIGYLDGTREKLMPEPICIEGDKNDIPIDIAM
                     QYNDSFAENIHSYVNNINTHEGGTHLAGFRRALTRTLKAYAEKSGLLAKLKFDISGDD
                     FREGLTAIISVKVMEPQFEGQTKTKLGNNEVMGAVDQIVSELLTYYLEEHPKEARTIV
                     NKVILAATARHAARKARELVQR"
     gene            1455..1841
                     /locus_tag="PLI16_09980"
     CDS             1455..1841
                     /locus_tag="PLI16_09980"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HOH84927.1"
                     /translation="MFWYHLLDYFFLSFHTVFTLFNILGWIWKKTRLANFITLTLTAF
                     SWFGLGIFYGFGYCFLTDWHWQVLHKLGHHDLPYSYIKYLIFRLLGINVNEELVETLT
                     IVVFVLAFMLSIWFNFAKKIFFRKKP"
BASE COUNT          474 a          422 c          425 g          663 t
ORIGIN      
        1 cgttgcacca gttcccgcgc tttacgggcg gcgtggcgtg ctgtagcggc cagtataacc
       61 ttgttgacaa tggtacgtgc ttctttcggg tgttcttcga ggtagtaggt aaggagctcg
      121 ctgacaatct gatctacagc gcccatcact tcgttattgc caagttttgt cttggtttgt
      181 ccttcgaact gtggctccat taccttgacg gaaataatgg ctgtaaggcc ctcacgaaaa
      241 tcgtcgccgc tgatatcaaa tttcagtttg gccagcaacc ctgatttttc ggcataggct
      301 ttcagcgtgc gtgtgagcgc cctgcggaac ccggccaggt gggtgccacc ctcatgggtg
      361 ttgatgttat ttacatagga atggatattt tcggcaaaag aatcgttata ctgcatggcg
      421 atatcgatgg ggatgtcgtt tttgtcgcct tcaatacaaa tgggttcggg catcagtttc
      481 tcgcgtgtgc cgtcgaggta tccgataaaa tctctcaggc cgtgttcgga ataaaattct
      541 tcattaatgg actgcccatt ttcgtctttt tcgcgcatat cagtaagggt cagcctgact
      601 tttttgttga ggtaggctag ctcgcgcagg cgtgcagcca gcagttcgta gctgtattcc
      661 gttacataaa agatagcttt atctggtttg aaggtaatgg tggtacctgt tttttccgac
      721 tcgccgattt ctctgacggc atattgagga tcaccgcatt tatattcctg ttcaaaaatc
      781 tttccttcac gaaatacagt tactttcatg tcggaggaca gcgcattgac gcaggacaca
      841 ccaacgccgt gcaaaccacc ggatactttg taggagcctt tgtcgaactt tccgcctgca
      901 tgaagcaccg tcataactac ttccaatgcc gaacggtgtt cctgttcgtg catgccggta
      961 gggatgccgc ggccattatc ttctacggtt attgaattat ctgtgtttat ggttacgtct
     1021 attctatcgc aatagccggc taaagcctca tcgatagagt tgtccactac ttcaactacc
     1081 agatggtgta accccctgct cccggtgtct ccaatataca tcgaaggccg cttgcggaca
     1141 gcttccaggc cttctaaaac tttaatacta tcggcgtcat aatttccgtt cccgtttccg
     1201 tttccgtttg tgccaacaac ttcattttct ttgtcgctca taatcaatta cttataaaat
     1261 tttatttgta aacaaagata cgcatttttt ttgtttcaat ttcaaagttt tttcataaaa
     1321 atctgcagaa gttattaaca ggttttagta tgtgtaaaca tcagatattc agtgctataa
     1381 atcattgttg taccattaat ttttgtttac cggaaaattg ttgtattttt ataaaaataa
     1441 ttgaacgaat agtcatgttt tggtatcatc ttcttgatta tttttttctt tcgtttcata
     1501 cggtttttac cctgttcaat attttgggtt ggatatggaa aaaaacaaga ctggcaaatt
     1561 tcattacgct gacgctcacg gccttctcat ggtttggctt agggattttt tacggttttg
     1621 gatattgctt tcttaccgac tggcactggc aggtgctgca caaactcggg catcacgacc
     1681 tgccatattc atatatcaaa tacctcatat tcaggttgct cggcatcaat gtgaacgaag
     1741 agctggtcga aacgctaacc atagttgttt ttgttcttgc ttttatgctt tcgatatggt
     1801 ttaatttcgc aaaaaaaatt tttttcagaa aaaagccttg aaaacataat tcttacgtaa
     1861 tttgttcttt taaaaaagta aggtattgtc aggcgagtat aaagagtatg tattgtatgc
     1921 gctgccactg gtggtgctgg ttacgaacag aattccattt gttggaaaat ttctccggat
     1981 tatc
//
LOCUS       DAOSFP010000404         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_57438_length_1982_cov_3.854177, whole genome shotgun sequence.
ACCESSION   DAOSFP010000404 DAOSFP010000000
VERSION     DAOSFP010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57438_length_1982_cov_3.854177"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PLI16_09985"
     CDS             complement(<1..625)
                     /locus_tag="PLI16_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84928.1"
                     /translation="MNQRRRNKIDEIVDYLEKKAVQDPASAKAIAENINFFKATYAVH
                     SFYKWLFSTAPAIKAENLKELVEFDAVSPYFNELMTALTATEKRIFPGLIRPVVKKIT
                     QLIRDKNYSVIISIGAGAMEVEKQVIKKLVRLGNQEPVIFIGIDKSDISRSLARENLT
                     GSPDTIILERNLLNEAMLKEINRETRSLYTVVLCNNDIFSLDKYFTGT"
     gene            complement(641..1039)
                     /locus_tag="PLI16_09990"
     CDS             complement(641..1039)
                     /locus_tag="PLI16_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84929.1"
                     /translation="MDKDQVPQDKNHYLDKNFKTVVYALDPDGKYTKVPSLGWEPEHF
                     AQKQAWDSVAEDIEIIKKNVIAGNVSPIAYFIEKYHFSLRRLSHLTGFSKRQIKKHFR
                     PEVFSALHSTDLGMYARAFQVPVDQIVKPF"
     gene            complement(1049..>1982)
                     /locus_tag="PLI16_09995"
     CDS             complement(1049..>1982)
                     /locus_tag="PLI16_09995"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH84930.1"
                     /translation="FFSITKNCNLNCKGCYQKAQNRAGDKDMSTEKIISILQEGQALG
                     SSVIGVLGGEPFTRKDFFTITGSFKKMLFATFTNGMLIDEAMIAKLRKNKNIIPIISN
                     EGPECETDNRRGAGVYSHFRSIAKKFKANHIIFGVSFTVTSKNFGMLTDETFIKELIS
                     DGSSLFFYVNYKPVDGISQELSLNKDQLKVLEDYTYTFREKYKTLYFSPLAEKQFGGC
                     IGAGKGLIHINFDGGIEPCPFAPYSDVNLNNVSLKEALNSKLLRTIRDQHAWLEQNNN
                     CGCSLWENRDWLKSLTKEKEPVSEEIAEEIASAV"
BASE COUNT          482 a          423 c          423 g          654 t
ORIGIN      
        1 ttgtccccgt aaagtatttg tcgagcgaga aaatatcgtt gttgcataac actaccgtat
       61 aaaggctgcg ggtttccctg ttaatttctt tcaacatggc ctcattgagc aggtttcttt
      121 ccaggattat ggtgtcgggg ctgccggtaa ggttttctct tgccaggcta cggcttatgt
      181 ctgatttgtc gatgccaatg aaaattacag gttcctgatt tcccaggcgg acaagttttt
      241 ttatcacctg tttttcaact tccatggctc ccgccccgat gctgataatc acactgtagt
      301 ttttatccct gattaattgt gtgatttttt tgaccacggg cctgattaat ccggggaata
      361 tccttttttc tgtggcggtt aaggcggtca tcaattcatt aaaatacggg cttaccgcgt
      421 cgaattccac cagttctttg agattttcgg cttttatggc aggggcagtg gaaaacagcc
      481 atttataaaa ggaatgtacg gcatacgtgg ctttaaaaaa attgatgttt tcagcaatag
      541 ctttggccga agcagggtct tgcacggctt ttttctccag atagtccact atctcatcaa
      601 ttttattgcg tcttcgttgg ttcattcgtg gttagcgaac ttaaaatggt tttacaattt
      661 gatccacagg tacctgaaaa gccctggcat acatcccgag gtcggttgaa tggagtgctg
      721 aaaaaacttc gggtctgaaa tgctttttta tctgacgttt agagaatcct gtcaggtgcg
      781 acaaccttcg cagcgagaag tggtactttt cgataaaata ggcgattggg cttacgttgc
      841 ctgcaattac attctttttt attatttcaa tatcttcggc caccgagtcc caggcttgtt
      901 tttgagcaaa atgttcaggt tcccagccca agctcggcac cttcgtgtat ttgccgtcag
      961 ggtccagggc atataccacg gttttaaagt ttttgtccag gtaatggttt ttatcctgtg
     1021 gaacttgatc tttgtccata tatcattgtt atacagcgct ggcaatttct tccgcaattt
     1081 cttcagagac aggttccttc tctttggtaa gagatttcaa ccaatcgcgg ttctcccaaa
     1141 gcgaacatcc acaattatta ttttgctcca gccatgcatg ttggtcacgg atggtacgaa
     1201 gcaacttcga attcagggct tctttaagtg aaacgttgtt caggtttaca tccgaataag
     1261 gagcaaacgg acaaggctct atgcctccat caaaattgat atggatcaaa ccttttccgg
     1321 cgccaatgca tccgccaaac tgcttttctg ccaaaggaga aaaatacagg gttttatatt
     1381 tttcccggaa cgtgtaggtg taatcttcca gaacctttaa ctggtctttg ttgagcgata
     1441 actcttgcga aatcccgtca acaggcttgt agtttacata aaaaaacaaa ctgctgccat
     1501 cacttatcag ttctttaata aatgtttcat cggtaagcat accgaagttc ttactcgtaa
     1561 ccgtaaatga tactccgaag ataatgtggt tggctttaaa cttctttgca atacttctga
     1621 aatggctgta aaccccggcg cctctcctgt tgtcggtttc acattcaggg ccttcgttgc
     1681 taatgatagg aatgatgttt ttgttttttc tgagttttgc tatcatggct tcatctatca
     1741 gcatcccgtt tgtaaaggtt gcaaataaca tttttttaaa actgccggtg atggtgaaaa
     1801 aatctttgcg ggtaaaaggt tctccgccca gcacgccgat gaccgatgaa cccaaagctt
     1861 gtccttcctg gagtatagag atgatttttt cggtgctcat atctttatct ccggcacggt
     1921 tttgggcttt ctgataacat cctttgcagt tcaggttgca gtttttggtg atactgaaaa
     1981 aa
//
LOCUS       DAOSFP010000405         1980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_57541_length_1980_cov_2.292468, whole genome shotgun sequence.
ACCESSION   DAOSFP010000405 DAOSFP010000000
VERSION     DAOSFP010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57541_length_1980_cov_2.292468"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1186)
                     /locus_tag="PLI16_10000"
     CDS             complement(74..1186)
                     /locus_tag="PLI16_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84931.1"
                     /translation="MEIKKAKFIPVLILAAVLLICFAFTSKPHFTIKVINQKPDPWEE
                     QLNVLNNLVMQTSSINLLHGLSLSKEQAQRLIPLANQVDQLNLNLSPNTTYYNNELQK
                     ICNTYKNLIGALMQNENLSDTLKNEVMRAREKHSEIIKRSLLATQTPGYQADGCRKCH
                     ASPEKFPSGNISAKDTPPITSEDRKEIDLAHVKGLFGEEGTLLLWNLRDQVDEVLTNE
                     QKYVFNQFRCCLIPQEDVSNPGIFGQSFVTNEWIEYFRKVRQLNDNEWQDYKSLFIIP
                     LADILEAKMPGIKKKDKKKMLDEAEKVINDSRNLDEVDFELQKENLCVSLSNALKTDL
                     LNGEANRQPEDRKFVAALFLLYPGSSAFYMKLCGQQ"
     gene            complement(1177..>1980)
                     /gene="amrS"
                     /locus_tag="PLI16_10005"
     CDS             complement(1177..>1980)
                     /gene="amrS"
                     /locus_tag="PLI16_10005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOH84932.1"
                     /translation="LPQSTAFSLATAGCNYTCLNCQNWQISQVSPFDTQNYDLFPKEI
                     VAYALKNNCKSIAYTYTEPVVFYEYVYDTAQLAHQEGLKNLFISNGSINEAPLRKLCK
                     YLDAANINLKSFKEDIYRKLNGGKLATVKKSLKIFKEEGVWLEITNLVVPGWTDDLDM
                     IKEMCDWLYDEGFADCPLHFSRFFPTYKLTQLPATPIDTLNKAHDIALAAGIRHVYIG
                     NVPGTEYDDTYCPKCKKKIIERRGYTILSNNIKDGKCGFCNEKINGVWK"
BASE COUNT          444 a          389 c          501 g          646 t
ORIGIN      
        1 tattagcagg caggtaacca tattgaaata atgtcgggag atattttttg ttgataaagc
       61 gggatttccc tatttactgc tgcccgcata atttcatgta gaaagcgctg ctgccggggt
      121 acagcaggaa caaggcggct acaaatttcc tgtcttcggg ttgcctgttg gcttcaccgt
      181 tcagcaggtc tgttttcagc gcattgctga ggcttacgca gagattttct ttttgcaatt
      241 caaaatcaac ttcgtctaag tttctggaat catttatcac tttttcggcc tcatcaagca
      301 ttttcttttt gtcttttttc ttaatgccgg gcattttggc ttcgagaata tcggcaaggg
      361 gtatgatgaa aagactttta taatcctgcc attcattatc gttcagttgc ctgacctttc
      421 ggaagtattc tatccattca ttggtaacaa aagattgtcc gaaaattccc ggattgctta
      481 cgtcttcctg gggaataagg cagcagcgga actggttaaa cacatatttt tgttcgttgg
      541 tgagtacctc atccacctgg tcgcggaggt tccacaaaag cagggtgcct tcttcgccaa
      601 acaggccttt gacatgtgcc agatctatct ctttgcggtc ttcggaggta atgggcgggg
      661 tgtctttagc cgatatgttg cctgaaggaa acttttcagg ggacgcatgg catttacggc
      721 agccatcagc ttggtatccc ggagtttgtg tggccagcag gctgcgcttt atgatttccg
      781 aatgcttttc gcgcgccctc atcacctcgt tttttaaggt atcgctgagg ttttcatttt
      841 gcataagcgc tccaatgagg tttttatagg tattgcaaat tttttgcagt tcattattgt
      901 aataggtcgt attcggtgac aaattcagat ttaattgatc tacctgattt gccagaggaa
      961 taagtctttg ggcctgttct ttgctgagtg agagtccatg cagcaggttg atggacgagg
     1021 tctgcataac aaggttattc aagacgttga gctgttcttc ccagggatcg ggcttttggt
     1081 ttatcacctt gattgtaaag tgcggctttg atgtaaaagc aaaacaaatc aacagcactg
     1141 cggcgaggat aaggacagga ataaactttg ctttttttat ttccatacgc cgtttatttt
     1201 ttcgttgcag aagccgcatt ttccatcttt gatgttgttg gaaaggatgg tgtatccacg
     1261 tcgttcgatg atttttttct tgcacttggg acagtatgtg tcatcgtatt ctgttccggg
     1321 tacattaccg atatatacgt gtcttattcc tgcggccagg gctatgtcgt gggctttgtt
     1381 gagggtgtct atgggggtgg cgggaagttg tgtgagctta taggtgggga agaatctgct
     1441 gaagtgcagc ggacaatctg caaagccttc atcataaagc cagtcgcaca tttcttttat
     1501 catgtcgagg tcgtcggtcc agccgggcac taccaggttg gtgatttcga gccagacgcc
     1561 ttcttctttg aaaattttaa gggatttttt aaccgttgca agtttgccgc cgttaagttt
     1621 gcggtagatg tcttctttga aacttttcag gttgatattg gcagcatcaa ggtatttgca
     1681 gagtttgcgc aaaggagctt cgttgattga gccgtttgaa ataaacagat ttttcaggcc
     1741 ttcctgatgt gccagttggg cggtgtcgta cacgtattca taaaacacca cgggttcggt
     1801 gtaagtatag gcaatacttt tgcaattgtt tttcagggca taggcaacaa tttcttttgg
     1861 aaacaggtcg tagttttggg tgtcaaaagg cgacacctga gagatttgcc agttttggca
     1921 gttcaggcag gtgtaattgc agcctgctgt ggccagcgag aaagcagtgc tctgtggcag
//
LOCUS       DAOSFP010000406         1978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_57616_length_1978_cov_2.984919, whole genome shotgun sequence.
ACCESSION   DAOSFP010000406 DAOSFP010000000
VERSION     DAOSFP010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57616_length_1978_cov_2.984919"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLI16_10010"
     CDS             complement(<1..859)
                     /locus_tag="PLI16_10010"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HOH84933.1"
                     /translation="MKSKKVVKIVFKALGIVVALLIVLAILLPIIFKKQIVETVKTEI
                     NKSVNAKVDFADYHLSLFLNFPDFTLGLDKLTIAGLDVFEGDTLADVDKLRVSVDLFS
                     VIKGDQYEINSIMLDGPRVNLIVRKDGKANWDIMKPAADTTAAAPEEPSTFRLAIDKL
                     TINNGTLTYDDEEANMHAKAISLNHTLKGDLTADFTSLDTKTTIDTLTFTYGGVSYLK
                     KANVELNAELDADLKNTKFTFKENELRVNNLFLGFDGWVAMPGENIDMDIKFNTRKTD
                     FSNILSLILA"
     gene            1000..>1978
                     /locus_tag="PLI16_10015"
     CDS             1000..>1978
                     /locus_tag="PLI16_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOH84934.1"
                     /translation="MEKRWVLKQLGSLELIQQLAKELNVSFPIANLLYQRGITTFEAA
                     RNFFRPELSHLHDPFLMKDMDKAIARIEKAIEKKEPMLIYGDYDVDGTSAVALVYSFL
                     KNYYPDIDFYIPDRYTEGYGISYQGIDYAAEKGISLVIALDCGIKAVEKIDYATEKGV
                     DFIICDHHRPGSEIPRAAAVLDPKRPDCSYPYEELSGCGIGYKLIQAFAKNRNIDEEV
                     LYKYLDLVTVSIASDIVPITGENRVLAFHGLKLINSNPRPGIEAVLQSSGMKRVKATS
                     HDGETQIFSRQLNINDLVFLFGPRINAAGRIESGRNSVQLLIADNMADAL"
BASE COUNT          554 a          461 c          409 g          554 t
ORIGIN      
        1 cagccagaat aagcgaaagt atattactga aatcagtttt cctggtattg aattttatgt
       61 ccatatcaat attttcgcca ggcatggcca cccatccgtc gaaaccaaga aacaaattat
      121 tcactcgtaa ttcattttcc ttaaaagtaa attttgtgtt tttcaggtca gcatcaagtt
      181 cggcgttaag ctctacattg gcttttttca ggtaggaaac tcccccgtaa gtaaaggtga
      241 gggtatcaat agtggtttta gtgtccagag aagtaaaatc ggccgtcagg tctcctttga
      301 gcgtatggtt caggctgata gctttcgcat gcatattggc ttcctcatca tcataagtaa
      361 gtgtcccgtt gttgatggtt aacttgtcta tggccagcct gaaagtagaa ggctcttccg
      421 gcgcagccgc ggtggtgtcg gcagcaggtt tcatgatatc ccagttcgcc tttccgtctt
      481 tccgtactat caggtttact ctggggccat ccagcattat ggagttgatt tcgtactgat
      541 cgcctttgat tacgctgaac aaatccactg aaacccgtag tttatcaaca tccgccagtg
      601 tatcgccttc aaatacatcc aatcctgcaa tagtaagctt atcgaggcct aaggtaaaat
      661 caggaaaatt caggaataac gaaagatgat aatcggcaaa atcaactttt gcattaacgc
      721 ttttatttat ctccgtcttg actgtctcta caatttgttt tttgaatatg atcggaagca
      781 ggatggccaa cactattagc agggcaacaa ctatacccag cgctttaaaa acaattttta
      841 ctaccttctt tgatttcata atttctgttt tttacaacac ataacaaatt tacataaaaa
      901 acataaattt tttgaggttt gaataaataa aaatcttgtc attataaaaa tgcaacgctt
      961 ataattagta cctttgcatt cctttaatag ggcaacagga tggaaaagcg ctgggtacta
     1021 aaacaattgg gaagtctgga attaattcaa caactggcca aagagttgaa tgtttctttt
     1081 cctattgcca atttacttta ccagcgaggc attaccacct ttgaggcagc cagaaacttt
     1141 ttcaggccag agctcagcca tctgcacgat cctttcctga tgaaagatat ggacaaggcc
     1201 attgcccgca tcgagaaggc cattgaaaaa aaagagccga tgcttattta cggcgattac
     1261 gatgtggatg gcacttccgc tgtagccctg gtttattcgt ttctgaaaaa ttattacccc
     1321 gacatcgact tttatatccc cgaccgctat accgaaggtt atggcatttc gtaccagggt
     1381 attgattatg ccgcggaaaa aggcatcagc ctggttattg ccctcgactg cggtatcaaa
     1441 gccgtggaaa aaattgatta cgctaccgaa aaaggcgttg attttattat ctgcgaccat
     1501 cacaggcccg gcagcgaaat tccgcgtgcg gcagctgtgc ttgaccccaa gcgtcccgac
     1561 tgttcttatc cttatgaaga actctcaggc tgcggcatag gctacaaact gattcaggct
     1621 tttgccaaaa acagaaacat tgacgaagaa gtactataca aatacctcga cctggtaacc
     1681 gtcagcatcg cttctgacat tgtgcccatc accggcgaaa accgtgtgct ggctttccac
     1741 ggactgaaac tcatcaactc aaacccccgt cccggcattg aagcggtgtt gcaaagcagc
     1801 ggaatgaaac gggtaaaagc cacctcccac gacggagaaa cacaaatctt ttcccgtcaa
     1861 ctcaatatca acgacctggt ttttttgttc gggccacgta tcaatgccgc cggtcgcatc
     1921 gaaagcggcc gtaactcggt ccagctgctc attgccgaca acatggcgga tgcgctgg
//
LOCUS       DAOSFP010000407         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_57649_length_1977_cov_4.352237, whole genome shotgun sequence.
ACCESSION   DAOSFP010000407 DAOSFP010000000
VERSION     DAOSFP010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57649_length_1977_cov_4.352237"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLI16_10020"
     CDS             <1..189
                     /locus_tag="PLI16_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb6"
                     /protein_id="HOH84935.1"
                     /translation="NEKIQEFSTTTDNLEEIFENREQIEIAKYYERLPKPSLVAINEY
                     LNNKLYFRNPLKENPTDF"
     gene            199..1407
                     /gene="coaBC"
                     /locus_tag="PLI16_10025"
     CDS             199..1407
                     /gene="coaBC"
                     /locus_tag="PLI16_10025"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOH84936.1"
                     /translation="MLNGKKILIGITGSIAAYKIPLLIRLLKKEGADVQVVVTPAAKD
                     FVTPLTLSTLSGHPVLCEGFNAGDGKWFSHIELGSWADIYLIAPVSANTLAKMANGLA
                     DNLLTTTYLAAKCPVFFAPAMDLDMYKHPATQKNVNTLCAFGHHLIAPREGELASGLC
                     GAGRLEEPEKIVEILDNFLKKKDLLKNKKILVTAGPTYESIDPVRFIGNHSSGLMGFE
                     LAAVCAENGADVTLITGPVTLATPYPHIRRVDVVSASQMYQQCLKAFPDADITIMAAA
                     VADYTPALVSKQKIKKSGTPLSINLKSTKDILAALGKIKRKDQLLVGFALETDNENEN
                     ALKKLKNKNLDLIVLNSLKDKGAGFKQPTNKVTLFNYKSEKKTFPLKSKHEVAIDIFN
                     EISKLRKDKI"
     gene            1404..>1977
                     /locus_tag="PLI16_10030"
     CDS             1404..>1977
                     /locus_tag="PLI16_10030"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOH84937.1"
                     /translation="MKKLVVLVLIIFSFNINPGCAQELFCSVSVNHSQISGSDKTVFE
                     EMQKAMLEFLNNRKWTNYSYRQEEKIECSFYFNISERSSDVDFKGTLQIQSRRPIFNT
                     SYNSPMLNIVDKDISFKYIQNQAMDYDDNNFYSNLTSLLAYYAYIIIGIDFDSYMMKG
                     GSQFYNKAQNIVQLAQNTADKGWKSFESRKN"
BASE COUNT          645 a          455 c          379 g          498 t
ORIGIN      
        1 aacgaaaaaa ttcaggagtt ctccacaaca accgacaacc tggaagaaat atttgaaaac
       61 cgggaacaga tagaaattgc caaatattac gaaagactcc ccaaaccatc gctggtcgct
      121 atcaacgagt acctcaacaa caaactgtat tttcggaatc cgttaaaaga aaaccctact
      181 gatttttaat cttttaggat gctaaacgga aaaaaaatac ttattggcat taccggaagt
      241 attgccgcct ataaaatacc cctgctaatc cgtctgctga aaaaagaagg agcggatgtt
      301 caggtggtgg tgacccctgc agccaaagat tttgttacgc cacttacatt gtcaacactt
      361 tccggacatc cggtattgtg cgaaggtttt aatgccggtg atggaaaatg gttcagccat
      421 atcgaactgg gtagctgggc agatatttat cttatagccc ctgtttcggc caataccctg
      481 gcaaaaatgg ctaacggact ggccgacaac ctgctaacta ccacttatct tgcagccaaa
      541 tgtcctgtgt tttttgctcc cgccatggat ctggatatgt acaaacatcc ggccacgcaa
      601 aaaaacgtca ataccttatg tgctttcggc caccacctca ttgccccgcg cgagggtgaa
      661 ctggccagcg gcctgtgtgg cgccggcagg ttggaagaac ccgaaaaaat tgttgaaatc
      721 ctggacaatt tccttaaaaa aaaagacctt ttaaaaaata aaaagatcct ggttacagca
      781 ggacctacct acgaaagcat tgatcctgtg cgttttatcg gcaaccactc ttcgggactg
      841 atgggttttg aacttgctgc cgtatgtgct gaaaacggtg ccgacgtaac cctgattacc
      901 ggtccggtaa ccctcgcaac accgtatccg cacatccgga gggttgacgt ggtctcggcc
      961 agccaaatgt accagcaatg ccttaaagct tttcctgatg ccgacatcac tatcatggcc
     1021 gccgccgttg ccgattatac ccccgcattg gtcagcaaac aaaaaataaa aaaatcagga
     1081 acgcctctat ccataaatct gaaatcgact aaagatatcc ttgctgcgct gggcaagatt
     1141 aaacgaaaag accaactact ggtgggtttt gccctcgaaa ccgataacga aaacgaaaac
     1201 gcactgaaaa aactaaaaaa caaaaacctc gacctcatcg ttttaaactc acttaaagac
     1261 aaaggagccg gttttaaaca acccacaaac aaggttaccc tgtttaatta caaatcggaa
     1321 aagaaaactt ttccacttaa atcaaaacat gaagtagcaa tagatatttt taacgaaatt
     1381 agcaaattaa gaaaagataa aatatgaaaa aacttgttgt attggttttg attatcttct
     1441 cattcaatat caacccgggt tgtgcccagg aacttttttg cagtgtgtct gtcaaccatt
     1501 cccaaataag cggtagcgac aaaacagttt tcgaggaaat gcagaaggcc atgctcgaat
     1561 tcctaaataa ccgtaaatgg accaactatt cttaccgcca ggaagaaaaa attgaatgtt
     1621 cgttctattt caatatctcc gaaagaagct cggatgttga ctttaaaggt actttgcaga
     1681 tacagtcacg caggccgatc ttcaatacct cctataattc gcctatgctc aatattgtgg
     1741 acaaggatat ttcttttaaa tacatccaga atcaggcaat ggattatgat gataacaact
     1801 tctattcaaa ccttacctca ctgctggcat attacgcata catcattata ggcattgact
     1861 ttgattccta tatgatgaaa ggtggcagtc aattttacaa taaggctcaa aacattgtac
     1921 aactggctca aaatactgcc gacaaaggct ggaaatcatt tgaaagccgt aaaaacc
//
LOCUS       DAOSFP010000408         1969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_58010_length_1969_cov_3.810867, whole genome shotgun sequence.
ACCESSION   DAOSFP010000408 DAOSFP010000000
VERSION     DAOSFP010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58010_length_1969_cov_3.810867"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..1217
                     /locus_tag="PLI16_10035"
     CDS             372..1217
                     /locus_tag="PLI16_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOH84938.1"
                     /translation="MYTIEELKNMATQVRRDLLRMIHAVQSGHPGGSLGCADFLTCLY
                     FGIMEHKPQPFSMDGRGEDLFFLSNGHLSAAWYSVLARSGYFDVSELATFRKLGTRLQ
                     GHPTTAEHTPGVRVASGSLGQGMSVAIGAALAKKQNEDTHLVYSLHGDGELEEGQIWE
                     AVMFAAAKKVDNIISVIDYNGKQIDGPVDEVLSLGDLKAKFLAFGWKVLEMNGNDMEQ
                     IQITLKNAQSHTGKGVPVVILMKTEMGYGVDFMMGTHKWHGSPPSDEQLANALAQLPV
                     TLGDY"
     gene            1228..>1969
                     /locus_tag="PLI16_10040"
     CDS             1228..>1969
                     /locus_tag="PLI16_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOH84939.1"
                     /translation="MRLASLKNFLVFIVALVLSGQVFSQITISEKEKPKPTPVTRILF
                     VYDASQSMFGVWQSDRKISIASKLMSELLDSLKTVANLHLGLRVYGHQKYYPPQDCDD
                     TRLEIPIGEGNIEKIKNKLKTVNPRGTTPIAASLEAAGKDFPPCDNCRNIIILITDGL
                     EECGGDPCAVSLALQKKGVVLKPFIIGIGKNFKESFDCVGTYFDASTEKEFSKALNAV
                     ISQALNSTTAQINLLDSYGKPTETNVGMT"
BASE COUNT          558 a          397 c          471 g          543 t
ORIGIN      
        1 tgacaaaggt ataacttttc ctaattatac taattcgggg aaatgaatgt gtccaatcat
       61 tttttgaggt gctgttggag attgttttat tgtaaataat tcaactttta ctattcagaa
      121 aaaaaattgt aaaaacggtt gtatgctcgt gcgtccatca ggatgggtaa tgtgatgccg
      181 taattaagaa taacccttgt caataccata tgtgttatgg tgaataaccc cggtatgcta
      241 ttttgcaagt gtgtaaactg gctaaactta atgtaataaa ttgttattca attttttaaa
      301 aaacttattc aatacttttt aacaatcaaa aaacaggcag ataaaaacta tatttttgaa
      361 aaaaattgta tatgtacact attgaagaac taaaaaacat ggctactcag gtgcggcgcg
      421 atcttttacg catgatccac gctgtgcagt cgggtcatcc cggcggttcg ctgggttgtg
      481 ctgattttct cacctgcctt tatttcggga ttatggaaca taagccgcaa ccattcagca
      541 tggatggcag gggcgaagat cttttttttc tttccaacgg gcatctttct gccgcatggt
      601 acagcgtttt agcccgttca gggtattttg acgtcagcga attggctact ttcaggaagc
      661 ttggaacccg tttgcagggt catccgacca cggccgaaca tactcctggt gtgcgggtcg
      721 cctccgggtc gttggggcag ggaatgtcgg tggctattgg cgccgccctg gcaaaaaaac
      781 aaaatgagga tacacacctt gtttacagtt tacatggcga tggcgaactg gaagaaggac
      841 aaatatggga agctgtgatg tttgccgcgg ccaaaaaagt tgataacatt atatccgtga
      901 ttgactacaa cggcaaacag atagatggtc ctgtggatga agtattaagc ctgggggatt
      961 tgaaagccaa attcctggcc tttggttgga aagtactgga aatgaatgga aacgacatgg
     1021 agcaaattca gatcacattg aaaaatgctc agtcgcatac tggcaagggt gttcctgtcg
     1081 ttattctgat gaaaaccgaa atgggctatg gcgttgattt tatgatggga acccataaat
     1141 ggcatggcag cccaccgagt gatgaacaac tcgccaatgc actggcccaa ttgccggtta
     1201 cactgggcga ttattaaaat gacaactatg agattggcat cattaaagaa ttttctggta
     1261 ttcatcgtgg cactggtgtt gtcggggcag gttttttcac agattaccat ttcggaaaaa
     1321 gaaaaaccca aacctacgcc ggttaccaga atattgtttg tttatgatgc ctcacaaagc
     1381 atgtttggcg tgtggcagag cgaccgcaag atatccattg ccagcaagct gatgtcggag
     1441 ttgctcgaca gtttgaaaac cgttgccaac ctgcatctgg gccttcgtgt ttacgggcat
     1501 cagaagtatt acccgcctca ggattgtgat gatacgcgcc ttgaaatccc tatcggagaa
     1561 gggaatatcg aaaaaattaa aaacaagtta aagacggtca acccgcgggg aaccacgccc
     1621 atagccgctt cgctggaggc tgcaggcaaa gattttcctc cttgcgacaa ttgccggaat
     1681 attatcattt tgatcaccga tggtctcgag gagtgtggcg gcgacccttg tgctgtttca
     1741 ctggcgttgc agaaaaaggg ggtagtccta aaacctttta ttatcggcat cgggaaaaat
     1801 ttcaaagaat ctttcgactg tgtaggaact tattttgacg cctccaccga gaaagaattt
     1861 agcaaagcgt tgaatgccgt tatttcgcaa gcgctcaatt ccaccaccgc gcagatcaat
     1921 ctgctggata gctatggtaa accaacagaa accaatgtcg gtatgacgt
//
LOCUS       DAOSFP010000409         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_58134_length_1966_cov_4.342752, whole genome shotgun sequence.
ACCESSION   DAOSFP010000409 DAOSFP010000000
VERSION     DAOSFP010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58134_length_1966_cov_4.342752"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLI16_10045"
     CDS             complement(<1..201)
                     /locus_tag="PLI16_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOH84940.1"
                     /translation="MKKNNDVSLKTAEYLLQIKAIKLNLQKPFTWASGWKSPIYCDNR
                     KTLSYPKIRTYIRQRFSTLIQEE"
     gene            200..838
                     /locus_tag="PLI16_10050"
     CDS             200..838
                     /locus_tag="PLI16_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOH84941.1"
                     /translation="MSQSYKVYINDKEIFFGNPESVVSKYNKDNTVSVLDKPSADSLR
                     IAIAGFVADPHLQQLMIVSENPKEVFREFRKDYKEIRAAGGLVRDKDMRVLLIFRRGK
                     WDLPKGKIDTDERKKHAALREVREETGLDQLFILSRIAKTYHIYTEKNDRILKKTYWY
                     EMAAINPGTLVPQTEEDITGIRWFEPDKLDEVFSNTFPLVADLLKENLSAQA"
     gene            complement(918..1841)
                     /locus_tag="PLI16_10055"
     CDS             complement(918..1841)
                     /locus_tag="PLI16_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84942.1"
                     /translation="MKTLHISRICVMLIAVLALAFTSCKKDKDPDTTTLQQFVSDENR
                     VAAADDEIMKDINSVLSGSGQKATNTFPCNVTVDSSVIVGDTIIYSITFNGLNCSGRW
                     NRVGQAEVRKHVNTHWPDAGATVYVKYLNVLFTRVSDNLTVTLNGKRIFTNVSGGRII
                     NLGTTATSVVHRVTGSLIATFNDNTTRTWTIARLITYTGTIGNLWVTIDGFGSADGYN
                     NLVVWGTNRSGELFYTQITQSVVRKESCNFDPCAGVKVHQIPADNKQATFTAGFDDNN
                     LPVDVNGSACPTKYRIDWIWNGNSGTLYKYL"
BASE COUNT          548 a          430 c          423 g          565 t
ORIGIN      
        1 ctcttcctgt atcaacgtac taaaccgctg cctgatatac gttctgatct tcggataaga
       61 gagggttttt ctgttatcgc aatagatggg cgacttccat cccgaagccc aggtaaaagg
      121 tttttgcaga ttcagtttta ttgctttaat ttgcaataag tattctgccg ttttcaatga
      181 aacatcatta ttctttttca tgtcacaaag ttacaaagta tatataaacg acaaagaaat
      241 ttttttcgga aatccggaaa gtgttgtaag caaatataat aaagacaata cagtatctgt
      301 tttagacaag ccctctgcag attccttacg aatagcgata gcaggttttg ttgccgaccc
      361 gcatttgcag caactgatga tagtcagcga aaatcctaaa gaggttttcc gggagtttcg
      421 gaaagactat aaagagataa gggctgccgg cggcctcgtg cgcgacaagg atatgcgcgt
      481 gctgctgatt ttccgcaggg gaaaatggga tttgcccaag ggaaaaattg atactgacga
      541 aaggaaaaaa catgccgcct tgcgtgaagt tcgcgaggaa acggggctgg accaactttt
      601 tattctcagc agaatagcca aaacctatca tatttataca gaaaaaaacg accgtatcct
      661 gaaaaaaaca tactggtacg aaatggccgc catcaatccg ggtacactcg tgccgcaaac
      721 cgaagaagat atcacaggca tccgttggtt cgaacccgac aagctggacg aggtgttttc
      781 aaataccttt cccctggtag ccgatttgtt gaaagaaaat ctctcggccc aggcttagct
      841 gacaatacaa ttatttgttc aggataaaaa aaaggaccgg cttttaaacc agtcctttag
      901 gctatgctaa cagcacatta taagtattta tacaaagtgc cgctgtttcc gttccaaatc
      961 caatcgatac ggtacttggt cgggcaggca ctcccattga catctacggg gaggttgttg
     1021 tcatcaaaac ccgctgtaaa tgtcgcctgt ttgttatcgg caggaatctg gtgcacttta
     1081 acgccggcac agggatcgaa attgcatgat tctttgcgca ccaccgactg ggttatttga
     1141 gtgtaaaaca actctccact acggttggtt ccccatacta caaggttatt ataaccatcg
     1201 gcagagccaa atccgtcaat ggtaacccaa aggttgccaa tagtgccggt ataggtaatc
     1261 aggcgggcga tggtccatgt cctggtcgta ttgtcgttaa aagtagcaat cagcgagcct
     1321 gttaccctgt gcactacgga ggtggccgtt gtccccaggt tgattatcct tcctccgctg
     1381 acatttgtaa aaatcctttt tccattcagc gtaacggtaa gattatcgga cactcttgtg
     1441 aaaaggacat tcaggtattt cacgtacact gttgcccctg catcaggcca atgcgtgttt
     1501 acgtgttttc tcacttcagc ctgtcccacc ctgttccacc ggccggaaca attcagccca
     1561 ttgaaagtaa tgctgtaaat aatagtatca cccacaatta ctgatgaatc caccgtcaca
     1621 ttgcagggga aggtattcgt tgctttctgc ccgctgccgg agagcacgct gttaatatct
     1681 ttcatgattt catcgtcggc ggcagccact ctgttttcgt cggagacaaa ttgttgcagc
     1741 gtggtggtat cagggtcctt gtcttttttg caggaggtga atgctaaggc caatacagca
     1801 attagcatga cacatatccg cgaaatatgt aatgttttca tgatttttgg tattaatgat
     1861 tattaaatta attatgttat tatacttata attcatttta acctttaaag atacaatttt
     1921 ttttattatc cgcaacagta aactgcaaat aatttggctg cttttc
//
LOCUS       DAOSFP010000410         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_58284_length_1963_cov_3.786688, whole genome shotgun sequence.
ACCESSION   DAOSFP010000410 DAOSFP010000000
VERSION     DAOSFP010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58284_length_1963_cov_3.786688"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1847)
                     /gene="metG"
                     /locus_tag="PLI16_10060"
     CDS             complement(<1..1847)
                     /gene="metG"
                     /locus_tag="PLI16_10060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOH84943.1"
                     /translation="MVTTEKKYKRTTITSALPYANGPVHIGHLAGVYVPADIYARYLR
                     SKGEDVVFIGGSDEHGVPITIKARHEHCLPQDIVDKYHHIIKKAFEEFGISFDIYSRT
                     SNKIHHETSAEFFKTLYDKGEFIEKVTRQYYDEEAKQFLADRYITGTCPHCNNERAYG
                     DQCEQCGTSLNATDLINPKSVLSGNPPVLRDTKHWFLPLDKYEPWLRQWILEEHKEWK
                     TNVYGQCKSWLDQGLQPRAVTRDLDWGVKVPVEGAEGKVLYVWFDAPIGYISATKEWA
                     AANGKNWETYWKDKETRLLHFIGKDNIVFHCIVFPAMLHAEGSYILPDNVPANEFLNL
                     EGDKISTSRNWAVWLHEYLEEFPGKQDVLRYTLTATAPETKDNDFTWKDFQARNNSEL
                     VAILGNFVNRALVLTHKFYNGEVPACSTITEYDKQVLNDLAGFPATIAESLDAFRFRE
                     ALAETMNLARLGNKYLAETEPWKLINTDAERVKTIMNIALQICANLSIVMEPFLPFSA
                     AKLIGLLNVSKHTWHDAGRSDLLMPGHKISRAELLFEKIEDEAIDFQLKKLHDTKKIN
                     QANNIVLQAAKEAIQYDDFTRMDLRTATILEAEVVPKTKKLLKLKLDTG"
BASE COUNT          481 a          431 c          439 g          612 t
ORIGIN      
        1 atgccggtat cgagtttcaa ttttagaagt ttttttgttt taggcacgac ttcggcttcc
       61 agtatagtgg cagtcctcag gtccatacgt gtaaaatcat cgtattgtat agcttctttt
      121 gctgcttgta aaacgatgtt gttcgcctgg tttatttttt ttgtatcgtg tagttttttc
      181 aactgaaaat cgatagcctc atcttcaatt ttttcgaaca gtaattctgc tcggctgatt
      241 ttatgcccgg gcatcaacaa atcactacgg ccagcatcat gccaggtatg ttttgaaaca
      301 ttaagcaagc ctattagttt ggcagcagag aagggcaaaa acggttccat cacaatgctt
      361 agattggcac atatctgaag ggcaatattc ataatggttt tcacacgttc tgcatcggtg
      421 ttgattagct tccagggttc tgtttcagca aggtatttgt ttccgaggcg cgccaggttc
      481 attgtttcgg caagcgcttc acgaaaacga aacgcatcaa gactttcggc aatggtggca
      541 ggaaatccgg ccaaatcgtt caacacctgc ttgtcatact ctgtaatagt agagcaggca
      601 ggcacttcgc cattatagaa tttgtgtgtc aacaccagcg cgcggtttac aaaattgccg
      661 agaatagcca ccagttcact gttgttacgt gcctgaaaat ctttccaggt aaagtcgtta
      721 tcttttgttt ccggtgccgt ggccgtgagt gtatagcgca acacatcctg tttcccggga
      781 aattcttcca ggtattcgtg caaccatacc gcccagttgc gcgaagtgga tattttatca
      841 ccttcgaggt ttaaaaactc gttggccggt acattgtcag gcaggatata gcttccttcg
      901 gcatgaagca tggcggggaa aacaatacag tgaaacacga tattgtcttt gccgataaaa
      961 tgcagcagcc tggtttcttt gtctttccag taggtttccc agttttttcc gttggcggca
     1021 gcccattctt tggtggcgga gatataacct atgggcgcgt caaaccacac gtacaacacc
     1081 ttcccttccg cgccttccac cggcaccttc acgccccagt caaggtcgcg cgtaacggca
     1141 cgtggctgca agccctggtc aagccatgat ttgcattgac catacacatt cgttttccat
     1201 tctttgtgct cttccaatat ccattgtctg agccagggtt cgtatttgtc gagaggcaaa
     1261 aaccagtgtt tggtatctct cagtacaggc ggattcccgc ttaacaccga ttttggattt
     1321 atcaggtcgg tggcattgag cgaggtgccg cattgttcgc actggtcacc gtaagccctt
     1381 tcgttattgc aatgcggaca agtccctgta atatagcggt cggccaaaaa ctgcttagct
     1441 tcttcatcat aatattgccg ggtaactttt tcaataaatt cacctttatc gtaaagtgtt
     1501 ttaaaaaact cggcagatgt ttcgtgatga attttattgg aagtgcgtga ataaatatca
     1561 aaagaaattc caaattcttc gaaagctttt ttgatgatat ggtggtactt atccactata
     1621 tcctgaggca aacagtgttc gtggcgggct ttaatggtta ttggaacccc atgctcgtcg
     1681 gagccgccga taaaaacaac atcttcgcct tttgatcgta aatagcgggc ataaatatcg
     1741 gcaggcacat atacgccggc aagatggccg atatgtacgg gcccgttggc ataaggcagg
     1801 gcagatgtta tggtggtgcg tttatatttt ttttctgttg ttaccattta attgcgtgtt
     1861 tttaatgttt ttcgcaagat ttttataact gcaaaattaa ttatttcggc tgatttatat
     1921 ggataatccc gaattacgca ttaaattttt ctaacgcgta gca
//
LOCUS       DAOSFP010000411         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_58325_length_1962_cov_5.003146, whole genome shotgun sequence.
ACCESSION   DAOSFP010000411 DAOSFP010000000
VERSION     DAOSFP010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58325_length_1962_cov_5.003146"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1499)
                     /locus_tag="PLI16_10065"
     CDS             complement(123..1499)
                     /locus_tag="PLI16_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOH84944.1"
                     /translation="MEPLIANKTVINKQTENSACIEQELKWFEEVLNTRLKAYFNHDC
                     PYADIDEVPVPDACRFSGDYSDFIRTNQLLPAERICLMVAFIPLLKPQLFDLFNITNA
                     NTGQRFTEFGCVRGMQHNGLIPTFETLLFILAGDNIPKRLDYMDYFSQNHFLFKNKKL
                     NLDQHSDSEPGHSALIIPSADLTESLLKNKQYVPEFSMKFPARKISTEQQWDELVLSG
                     DVMAQINEIKAWVELGDKMLDEWNLRRKMKPGYRSLFYGPSGSGKTFTATLLGKYTGR
                     EVYCVDLSMVVSKYIGETEKNLSKIFEGAEHKNRILFFDEADALFGKRTNIKDSHDRY
                     ANQEVSYLLQRVEDYHGLVVLSTNLKANIDEAFARRFQSVIHFPVPDAGQRELLWNNI
                     FSEKTTLEKNTNIREIAEKYELTGGAIVNVVRYCSLMAMSRNNNIILHEDIINGIKKE
                     FHKEGKTI"
     gene            complement(1487..>1962)
                     /locus_tag="PLI16_10070"
     CDS             complement(1487..>1962)
                     /locus_tag="PLI16_10070"
                     /inference="COORDINATES: protein motif:HMM:NF039901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HOH84945.1"
                     /translation="ILHPLFENLFQHVGLIDGHGLFKSEASRHRAVHLLHSLTGIAGK
                     HFEYRLPLNKIICGMDINMPLDPAFRIKKQEREELNDLLKAMLTHWSALKSTSIRGLQ
                     ESFIRRRGTIEKSGNDWIVRVENTGIDILLNDLPWGVQIMKFPWNNYIIFVEWNH"
BASE COUNT          467 a          442 c          453 g          600 t
ORIGIN      
        1 aagggggagt tccgacagtt gtattgtact ggcaaatccg gggttttccc tggccttgtt
       61 tgggtggcat ggagcctgtt tttcggtttg tatgctggct tttgacatat ggtaacttaa
      121 tttcagatgg tttttccttc tttgtgaaat tcctttttta taccgttaat gatatcctca
      181 tgcaggatga tgttgttatt cctgctcatg gccatcagcg aacaatagcg tacaacattt
      241 actatggctc caccggtgag ttcatatttt tctgcaattt cccttatgtt ggtgtttttt
      301 tccagtgtgg ttttttcaga aaagatgttg ttccataata gttcgcgttg tccggcatcg
      361 ggaaccggaa aatgtatcac gctttgaaag cggcgggcaa aggcctcgtc gatattggct
      421 ttcaggtttg tagagagcac taccagtcca tggtaatctt ccacccgttg caacagatag
      481 gaaacttcct ggttggcata gcggtcgtgc gaatccttga tgttggtacg tttgccaaac
      541 agtgcatcgg cctcatcaaa aaacaatatc cggtttttat gttcggcgcc ttcaaaaatt
      601 ttcgataggt ttttttccgt ttccccaata tatttcgaca ccaccatgga taaatccacg
      661 caatagacct cccgcccggt gtactttccg agcagtgttg ccgtaaaggt ttttcccgat
      721 cccgaaggcc cgtaaaacag gcttctgtag cccggtttca tcttacggcg cagattccat
      781 tcgtcgagca tcttgtcacc aagctccacc caggccttga tttcgttgat ctgcgccatc
      841 acatcacctg acaacaccag ctcatcccat tgttgttcgg tgcttatttt acgagcagga
      901 aatttcatgg aaaactccgg cacgtactgt ttgtttttca acaacgattc cgtaaggtct
      961 gccgagggga taatcagggc gctgtggccg ggctcgctgt cggaatgctg gtcgaggttc
     1021 agttttttat ttttgaacaa aaagtgattt tggctgaagt aatccatgta atcaagcctt
     1081 ttgggaatat tgtcacccgc aagaataaac aacagggttt cgaaggtagg gatcaggccg
     1141 ttgtgttgca ttccccttac acagccaaac tcggtaaaac gctggccggt atttgcattg
     1201 gtgatgttga acaaatcaaa aagctgtggt tttagcagag gaataaaagc caccatgagg
     1261 cagatacgtt cggcgggcaa cagttggttc gttcttatga aatcgctgta atcgccggaa
     1321 aatctgcagg catctgggac ggggacctcg tcgatatctg catagggaca atcgtggtta
     1381 aagtaggcct tcagacgcgt gttgagcact tcttcaaacc atttcaattc ctgttcaata
     1441 caggcactgt tttctgtttg cttatttatc actgttttgt tagctattaa tggttccatt
     1501 ctacaaaaat gatatagttg ttccagggaa atttcataat ttgaacaccc catggcaggt
     1561 cgttgagcaa aatgtcaata cctgtgtttt ctacccgcac tatccagtcg ttgccacttt
     1621 tttctatggt tcccctgcgc cggatgaatg attcctgcaa cccgcgaata gaggtgcttt
     1681 tcagggcact ccaatgtgtc agcatggcct tcagcaaatc gttcagttct tcgcgttcct
     1741 gtttttttat acggaaagca gggtctaaag gcatgtttat atccataccg cagattattt
     1801 tattcagcgg cagccggtat tcaaagtgtt ttcccgcgat gcctgttaaa gaatgcaaaa
     1861 ggtgcacagc ccggtgtcgg gaggcttcag atttaaaaag cccatgcccg tcaatcaatc
     1921 ccacatgctg aaaaagattt tcgaacagcg gatgcaggat ga
//
LOCUS       DAOSFP010000412         1960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_58416_length_1960_cov_3.420472, whole genome shotgun sequence.
ACCESSION   DAOSFP010000412 DAOSFP010000000
VERSION     DAOSFP010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58416_length_1960_cov_3.420472"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /gene="greA"
                     /locus_tag="PLI16_10075"
     CDS             <1..364
                     /gene="greA"
                     /locus_tag="PLI16_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOH84946.1"
                     /translation="RDKGDLSENAEYDAAKDAKGMLELKISKLQELVNNAKIIDESML
                     DMSKVLILSTVELKNLSNGSKTTYTLVPENEANLKLGKISVNSPIAKGLLGKKAGDKV
                     EINVPAGILKFEILSISY"
     gene            375..773
                     /locus_tag="PLI16_10080"
     CDS             375..773
                     /locus_tag="PLI16_10080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOH84947.1"
                     /translation="MASIFSKIVAGDIPCYKIAENDKFFAFLDINPLAKGHTLVIPKQ
                     ETDYLFDLDDEQYKDLFLFAKKIAIAIKKTIPCKKVGIAVVGLEVAHAHIHLIPLNDV
                     YDIDFKKDKLKLTPEEFGKIASGIAQAMGD"
     gene            complement(755..1345)
                     /locus_tag="PLI16_10085"
     CDS             complement(755..1345)
                     /locus_tag="PLI16_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOH84948.1"
                     /translation="MLIIENTLVADELAQCMFCCDYNACKGICCVEGDAGAPLEENEI
                     KILTSSLPELQKFMSVEGITAVAKQGVFVYDMEGKKVTPIIRGRDCAFLVYKNDVAAC
                     AIEMAYRKKKIALQKPISCHLYPLRIIDYQDFIAVNYHEWHICKPALKKGKKENIYLY
                     EFLKTPLIRRFGKGWYDELIKIAEYLRSKKNQSPMA"
     gene            1421..>1960
                     /locus_tag="PLI16_10090"
     CDS             1421..>1960
                     /locus_tag="PLI16_10090"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="An essential enzyme in the nonmevalonate pathway of
                     isopentenyl diphosphate and dimethylallyl diphosphate
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOH84949.1"
                     /translation="MVVYPNCKINIGLNIVSKRPDGFHNIESVFYPVGWCDMLEIIPS
                     AANSFIFDGFKINCPLEENLCYKAWQLLKRRFDIPNISLYLYKKIPFGAGLGGGSSDG
                     AFVLRTLNELFGLEMDRTAMEALASELGSDCPFFIDNLPCFVSGTGADIAPVELSLKG
                     YYLMVVKPAVSVSTVEAYRM"
BASE COUNT          574 a          387 c          421 g          578 t
ORIGIN      
        1 ccgggataag ggtgaccttt ccgaaaatgc tgaatatgat gctgctaaag acgctaaagg
       61 catgcttgaa ttaaaaattt cgaagctgca ggaattggtt aataatgcca aaattattga
      121 tgaatccatg ctggacatgt cgaaagtact tatactatcg acggtagaac tgaaaaacct
      181 gtccaacgga tcgaaaacca cctacaccct ggtacccgaa aacgaggcaa acctcaaact
      241 gggcaaaata tctgtaaact cgccaatagc aaagggcctg cttggaaaaa aagccggcga
      301 taaagtagaa ataaacgtgc ctgcgggcat cttgaaattc gaaattttat ccatttcata
      361 ttaaatcaga acctatggca agcatatttt caaaaattgt ggcaggtgat ataccttgct
      421 ataaaattgc agaaaacgat aaattttttg catttctgga tattaatcct ctggccaaag
      481 gccatacgct ggtaataccc aaacaggaaa ccgattactt gtttgatttg gatgatgaac
      541 agtataagga tttatttctt tttgccaaaa aaatcgcaat tgctatcaaa aagactattc
      601 cctgcaaaaa agtaggtatc gccgtggtag gccttgaggt ggctcatgca cacatacacc
      661 tgataccact caatgatgtg tatgatattg actttaaaaa ggacaaactg aagttaacgc
      721 ccgaagagtt cgggaaaata gcatcaggca ttgctcaggc catgggtgac tgattttttt
      781 tgctgcggag gtattcagct atcttgatca gttcgtcgta ccagcccttt ccaaacctgc
      841 gtatgagggg cgttttcaaa aattcataga ggtaaatatt ttcttttttg ccttttttta
      901 aggccggttt acagatatgc cattcatggt aattgacggc aataaaatcc tgataatcaa
      961 taattcttaa aggatacaga tggcaggaaa tgggtttttg taaggcgatt ttctttttac
     1021 gataagccat ttcaatggca caggcagcta catcattttt gtacactaaa aaagcacaat
     1081 cccttccacg gattatgggc gttacttttt taccttccat atcatacaca aaaacaccct
     1141 gttttgccac cgcggttata ccttccacac tcataaattt ttgtaattct ggcagggagg
     1201 aggtgaggat ttttatttcg ttttcttcga gcggtgcgcc ggcatcgcct tctacgcagc
     1261 atatgccttt acaggcgtta tagtcgcagc agaacatgca ttgggcaagt tcgtcggcaa
     1321 ccagggtgtt ttcaattatc agcataaaag aatgaaaact caaatgtaga aaaatacctg
     1381 atagggtata atgatttggt atatttttgt ataaaaaaac atggttgttt atccaaactg
     1441 taaaatcaat atcggcctga atatcgtttc aaaacgtccg gatggttttc acaatattga
     1501 aagtgttttt tatcctgttg gttggtgcga tatgctggag attataccgt cagcagctaa
     1561 cagctttatt tttgatgggt ttaaaatcaa ttgtcccctg gaggaaaacc tgtgctataa
     1621 ggcctggcaa ctgctcaagc gccgtttcga tatacccaat atcagtttgt atctttataa
     1681 aaaaattcct tttggtgcag gcctgggagg aggttcttcc gatggcgcat ttgtgctgag
     1741 aacgctgaat gaattatttg gccttgaaat ggaccgcacc gctatggagg ccttggcatc
     1801 ggaactgggt agcgattgcc ctttttttat tgacaacctg ccatgttttg tttccggtac
     1861 aggcgccgat atcgccccgg tggaactttc actgaaaggg tactacctga tggttgtcaa
     1921 gcctgcggtt tccgtttcaa cggtggaggc ataccgcatg
//
LOCUS       DAOSFP010000413         1931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_59756_length_1931_cov_4.121535, whole genome shotgun sequence.
ACCESSION   DAOSFP010000413 DAOSFP010000000
VERSION     DAOSFP010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59756_length_1931_cov_4.121535"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..870)
                     /locus_tag="PLI16_10095"
     CDS             complement(1..870)
                     /locus_tag="PLI16_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOH84950.1"
                     /translation="MNIVIIYAVITLSVIGLLSAVILFFIAQKFKVIEDPRIDEVAGL
                     LPGANCGGCGFAGCRNMAENIVKKESTEGYFCPPGGNDTMKAIAAVFGKVAEDKAPMI
                     AVVRCNGTCTNAVNKNEYDGLKSCYFMHNLYSGTNGCPYGCLGCGDCVTACQFDAMYM
                     DEQTGLPVVVQDKCIACGACVKACPRGIIELRNKGKKDRRIFVSCINKEKGAVARKNC
                     EVACIGCGKCVKECKFEAITMENNLAYINFEKCTLCRKCVESCPTGAIREVNFPERKK
                     KDADTPQEQPANN"
     gene            complement(898..1884)
                     /locus_tag="PLI16_10100"
     CDS             complement(898..1884)
                     /locus_tag="PLI16_10100"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOH84951.1"
                     /translation="MINLTTKYMGFELKNPLIVASSGLTKSPEKIRSFEEKGAAAVVL
                     KSLFEEQIMFETNHIMDYPDSYNNYPEALDYIQNFTKHNSVEEYMTLIKTVKKEVKIP
                     VFGSINCVSLNEWTTIARDIEKAGADALELNIFIMPSDLNLTGAEHEKMYFDIIAKIK
                     NTVKIPVAIKVGYYFSGLVNLLQKFSWTGIGALVLFNRFYTPDINIDTLHLQASNILS
                     TPAEIGNSLRWIALLSDKAGCDIAASTGIHDASGVIKQLLVGATATQLCSTLYLNGAG
                     RIQEILEDLTKWMSDKNYNQISDFRGKLSLKKAENPVMYHRVQYMKHLSDTD"
BASE COUNT          520 a          443 c          393 g          575 t
ORIGIN      
        1 ttaattgtta gccggttgtt cctgaggagt atcggcatcc ttctttttac gttccgggaa
       61 atttacttcc cggatagcgc cggtaggaca cgattcaacg catttacggc ataaggtgca
      121 cttttcaaaa ttgatgtagg caaggttgtt ttccatggta atagcttcga atttgcattc
      181 tttaacacat tttccgcaac cgatacaggc tacttcgcag tttttcctgg ccacagcgcc
      241 tttctctttg ttaatacaac tgacaaaaat ccggcggtct tttttgcctt tgtttctcag
      301 ttcgatgatg cctctggggc aagccttgac acaggcgccg caggcgatac acttatcctg
      361 aaccacaaca ggcaggccgg tttgttcatc catatacatg gcatcaaact gacaggccgt
      421 aacacaatcg ccgcatccca aacaaccgta aggacaccca ttggtgcctg aatacaaatt
      481 gtgcatgaaa taacaggatt tcaatccgtc atactcattt ttatttacgg cattagtaca
      541 ggtgccatta caacgaacca ccgctatcat cggggctttg tcttcagcca ccttcccaaa
      601 aacggctgca atagctttca tggtgtcatt tcctcccgga gggcaaaaat agccttcggt
      661 tgattctttt tttacgatgt tttcggccat attccggcag cctgcaaatc cacatccgcc
      721 gcaattggcg ccaggcagca agccggccac ttcatcaatg cgcggatctt cgataacttt
      781 aaacttctgg gcaataaaaa acaaaatcac tgcagacagc aacccaataa cgctgagcgt
      841 aataacagca tagataatta caatattcac gtgctatatt atttaatgtt aaacaaatta
      901 atcggtatcg gacaggtgtt tcatatactg taccctgtga tacatcacag gattttcggc
      961 tttcttcagg ctgagtttac ccctgaaatc acttatctgg ttatagtttt tatctgacat
     1021 ccatttagtc aggtcttcca atatttcctg aatacgtccg gcgccgttaa ggtataatgt
     1081 ggaacatagt tgggtagcgg ttgcaccgac aaggagttgt tttatcacgc cgctggcatc
     1141 gtgtatgcct gttgaagcag caatgtcgca accggcctta tccgacagca gggcgatcca
     1201 gcggagggaa ttgccgattt cggcgggggt gctgagtata ttgcttgcct gcagatgtaa
     1261 tgtatcaatg tttatatcgg gagtgtaaaa acggttaaag agtaccagag cccctatgcc
     1321 tgtccacgaa aacttttgta acagatttac caagccactg aagtaataac ccaccttaat
     1381 ggccaccggg atttttaccg tgttttttat tttcgcaata atgtcgaaat acatcttttc
     1441 atgttcggca ccggtaaggt taagatcgct gggcatgata aaaatattaa gctccagggc
     1501 gtcggcgccg gctttttcaa tatcacgcgc tatggtcgtc cattcgttga gcgaaacaca
     1561 attgatactg ccaaaaacag gaatttttac ttctttcttt acagtcttga taagcgtcat
     1621 gtattcctca acagaattgt gtttggtaaa attctgaatg taatcgagcg cctcgggata
     1681 gttattataa gaatccggat aatccataat atgattcgtc tcaaacataa tctgttcttc
     1741 aaagagcgac ttcagtacca ctgccgcagc acctttttct tcaaaagagc ggattttttc
     1801 aggcgatttc gtcaaaccgg aactggcaac tatcaaaggg tttttcaact cgaaacccat
     1861 gtatttggta gtcaaattta tcatggtaat cattttttaa acaggtgcaa aattatactt
     1921 tttattaact a
//
LOCUS       DAOSFP010000414         1928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_59893_length_1928_cov_4.265884, whole genome shotgun sequence.
ACCESSION   DAOSFP010000414 DAOSFP010000000
VERSION     DAOSFP010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59893_length_1928_cov_4.265884"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLI16_10105"
     CDS             complement(<1..193)
                     /locus_tag="PLI16_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84952.1"
                     /translation="MKKFLISLLVFSVQALFFSTQAQPWMQAPYLENNKSNLSNAGYQ
                     NATFYDIQKAFYSYWKDKPY"
     gene            375..>1928
                     /locus_tag="PLI16_10110"
     CDS             375..>1928
                     /locus_tag="PLI16_10110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HOH84953.1"
                     /translation="MSGKTFAEKILGAECGSIVFKKPDIVLSHDNTASINATFTKMGG
                     DKVADPDQLLIVLDHNAPPTNAKLANDYQKIRDIVAKQGIKKFHDVGNGICHQLMSLH
                     ARPNMVIVGSDSHTCTAGAFNAFSAGIDRTETAGLWKQGETWFRVPESIKITLKGKLN
                     AGVYAKDIALWIIGMMGSDGADYMSIEYHGEGVKSLSIADRMTIANLASEMGAKNAVF
                     PADEVLWNFLGAKTQGTWADTDAKYLKEFEINLNELFPVVAAPHHVDNVKAVSEVQGT
                     KLNQAIVGTCTNGRIEDIRIAASILKGKKIPAGFQLLVIPASMEIYKMAMQEGLVMDL
                     LQAGANFLSPSCGPCLGTGQGIPADGYNVISTANRNFLGRMGNEKASIYLASPATVAY
                     SALKGEISDPRGITGADKFPYSKEQSHTVQVKQGEDRYDKGVWGYHDIDNMNTDQMFA
                     GNLTYNINSSEGDKIMPYLFKGVDESFAERVKAGDIVIAGMNFGCGSSREHPAVGLSF
                     AGVQAVICKS"
BASE COUNT          554 a          396 c          477 g          501 t
ORIGIN      
        1 cataaggctt gtccttccaa tagctgtaaa aagctttttg aatatcataa aatgtcgcat
       61 tctgataacc ggcatttgac aggttgcttt tattattttc aaggtatggg gcttgcatcc
      121 atggctgggc ttgggtagag aaaaatagtg cctgaacaga aaatacaagt agtgagatga
      181 ggaatttctt catgagtatg gaatttaaga caaaattacg aaataatctc ttgattatgc
      241 tgtttgggct ttgctatatt tggatttatc aggaaataaa ccaaatttta tggaataata
      301 tcttatttgg caaaataatg tttttctaat aagaaataga ctataacttt gtagtaaatt
      361 ttaaaaaaac acttatgtca ggaaaaacat ttgccgaaaa gattctcggt gccgaatgcg
      421 gaagcatcgt tttcaaaaaa cccgatattg tgctgtcgca cgacaatact gccagtatca
      481 acgccacttt taccaagatg ggcggcgaca aggtggccga tccggaccaa ctcctgattg
      541 tgctcgacca caatgccccg ccaactaatg ccaaactggc caacgattat cagaaaatca
      601 gggacattgt tgctaagcag ggaataaaaa aatttcatga tgtgggtaat ggtatttgtc
      661 atcagctgat gtcattgcat gcccggccca atatggtcat agtgggtagc gatagccaca
      721 cctgtacggc aggagctttt aatgcctttt cggccggtat cgaccgcacc gaaactgccg
      781 gtctgtggaa acagggcgaa acctggttcc gcgtgccgga gtcgattaag attacgctga
      841 aaggaaaact caatgcagga gtttatgcaa aagatattgc cctgtggatt attggcatga
      901 tgggaagcga cggcgccgac tatatgtcga ttgaatatca tggcgaagga gtgaaatcgc
      961 tgagtattgc cgaccgcatg accatagcta acctcgcttc ggaaatgggc gccaaaaatg
     1021 ccgtattccc cgccgatgaa gtgttgtgga attttctcgg tgcaaagact caaggcacct
     1081 gggccgatac tgatgcaaaa tacttgaaag aatttgagat taatcttaac gaattattcc
     1141 ctgtggtggc tgctcctcat catgtggaca atgtaaaagc cgtttctgaa gttcagggga
     1201 caaaactgaa ccaggccatc gtgggcactt gcaccaacgg acgcattgaa gatatccgta
     1261 tagccgcctc gattttaaaa gggaaaaaga ttcctgccgg attccagtta ctggttatcc
     1321 cggcatcaat ggagatatat aaaatggcga tgcaggaagg cctggtgatg gatctgcttc
     1381 aggcaggtgc caatttcctc tcgccgtcat gtggtccatg tttaggcaca ggccagggga
     1441 taccagccga tggttataat gtgatttcta cggcaaacag gaatttcctc gggcgtatgg
     1501 gtaatgaaaa agcgagtatc taccttgctt cgccggccac cgtggcttat tccgccctga
     1561 aaggcgaaat cagcgatccc cgcggaataa ccggtgccga caaatttccg tactcgaaag
     1621 aacaaagcca tactgtgcag gtaaaacagg gcgaagaccg ttatgataaa ggagtgtggg
     1681 gataccacga tattgataat atgaataccg accagatgtt tgcaggtaac cttacctata
     1741 acatcaacag ctccgaaggc gataagatta tgccttatct tttcaagggc gtggacgaaa
     1801 gctttgccga aagagtgaaa gcaggagata ttgtgattgc cggcatgaat ttcggttgtg
     1861 gcagctcccg tgagcacccg gctgttggtt tgtcttttgc cggtgtgcag gcggttatct
     1921 gtaaatcg
//
LOCUS       DAOSFP010000415         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_60520_length_1915_cov_3.480645, whole genome shotgun sequence.
ACCESSION   DAOSFP010000415 DAOSFP010000000
VERSION     DAOSFP010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60520_length_1915_cov_3.480645"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PLI16_10115"
     CDS             complement(<1..177)
                     /locus_tag="PLI16_10115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOH84954.1"
                     /translation="MDLIKKYFPGISEEQQQLFGKLGPLYKEWNEKINVISRQDIDNL
                     YLHHVLHSLSIARLI"
     gene            265..1293
                     /locus_tag="PLI16_10120"
     CDS             265..1293
                     /locus_tag="PLI16_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOH84955.1"
                     /translation="MKKYIVLFVGLVMTLNVTGQSKMSVAGYIQKYQHIAVEEMKLYK
                     IPASITIAQGILESANGNSELARNANNHFGIKCKVEWTGEKYYYDDDAENECFRKYKS
                     DRDSYRDHSLFLTTRKYYTGLFSLDIMDYKAWAYGLKKAGYATEPKYAEMLIKIIEEN
                     LLFKLDSGQLIGTQEALAANNLPSAQQPLTPQTEVKRTPDSLNKVAKIREINNGPGDF
                     PDIQLSGKNREVFVNNGVKYIVAEKQDSYQSVADYFGLAEFDILRYNDIKNKKPLMPG
                     AKVYIESKKKSAAIDFHVAGPGETMQDISQLYAVQMKELYRKNRMKQGTQPRQGQKLW
                     LTKPMPVY"
     gene            complement(1466..1594)
                     /locus_tag="PLI16_10125"
     CDS             complement(1466..1594)
                     /locus_tag="PLI16_10125"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOH84956.1"
                     /translation="MGSGCDEEALRVISSMPVWKPGRQNGKEVRVQLNMPIHFKLL"
     gene            complement(1609..>1915)
                     /locus_tag="PLI16_10130"
     CDS             complement(1609..>1915)
                     /locus_tag="PLI16_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84957.1"
                     /translation="EEIPDNLSDLAEKAQNTSPNDTLAGDVDIPDEKPKPSVIDDDYS
                     TPPPTVVEEMPEFPGGEAARIKFLSENIKYPQIAREIGVQGPVFVTFVVEKMAASLE"
BASE COUNT          556 a          423 c          427 g          509 t
ORIGIN      
        1 tatcagccgt gcaatactca acgaatgcaa cacatgatga agataaagat tgtcaatatc
       61 ctgacgcgaa atgacattga ttttttcatt ccattccttg taaagagggc cgagcttccc
      121 gaaaagctgt tgctgttctt cgcttatgcc gggaaaatat ttttttatca gatccattgt
      181 tttaacaaat tgatgtggaa aattacataa aaacaacagg tagccgcaac aaaaacttca
      241 atacttttga acaaagtttt gtgcgtgaaa aagtatattg ttttgtttgt tggattggtg
      301 atgacgctga atgttacagg gcagtccaaa atgtcggtgg ccgggtatat tcagaaatac
      361 cagcatattg cggttgagga aatgaagctt tacaaaatac cggcaagtat taccatcgcc
      421 caggggatac tggagtcggc caacggcaac agcgaactgg cccgcaatgc caacaaccat
      481 tttggtataa aatgtaaggt ggaatggacc ggcgaaaagt attactatga cgacgacgcc
      541 gaaaacgaat gtttcagaaa atacaaatcc gacagggact cctaccgcga ccactcgctt
      601 tttctgacca cacggaagta ctataccggt ttgttttccc tcgatattat ggattataag
      661 gcctgggcct atggactgaa aaaagccggc tatgccacag agcctaaata tgccgagatg
      721 ctcataaaga tcatcgagga aaatttgttg tttaagcttg attccggaca attaattggt
      781 actcaggagg ccctggcagc gaataatttg ccttcggccc aacagcccct gacaccgcaa
      841 acagaggtaa aaagaactcc cgacagttta aataaagtag cgaagattag ggagataaac
      901 aatggcccgg gcgatttccc cgacatacag ttgtccggga aaaacagaga agtttttgta
      961 aataatggcg tgaaatatat tgtagctgaa aagcaagatt cctatcaatc cgtggcggac
     1021 tattttgggc ttgccgaatt tgatattctg cggtataacg atataaaaaa caaaaagcca
     1081 ttgatgccgg gcgcaaaagt ctatatcgaa tcaaaaaaga aatccgctgc cattgacttt
     1141 catgttgccg ggccgggaga aaccatgcag gacatctcgc agttgtatgc tgttcagatg
     1201 aaagaattgt acaggaaaaa ccgcatgaaa cagggtaccc agccgcggca gggacagaaa
     1261 ctgtggctca ccaaacctat gcccgtttat taaaaaaaga ggccgtataa aaaataaata
     1321 cagcctgaat ctgcttttat atttccctta gtacgtgcct gcctgcccgg ccagacaaac
     1381 tttgtgcccc ttcgtggttc aactgcctgt ttttccacaa agaggcacaa aggacttttg
     1441 atactatttt ttttagcgcc agcgactaca gcaatttgaa gtgaattggc atattaagtt
     1501 gtaccctgac ttcttttcca ttctgacggc cgggtttcca tacgggcata gagcttatca
     1561 cccgtaaggc ctcttcgtca catccgctgc cgatacctct cagtattttt actccagtga
     1621 tgctgccatt ttttcgacca caaaggtgac gaacacaggc ccctgaacac ctatttcacg
     1681 agcaatttga ggatatttga tgttttcgga gagaaatttt attctggccg cctcgccacc
     1741 cggaaattca ggcatttctt ccaccacggt gggcggaggt gtgctgtaat catcatctat
     1801 tacacttggt ttgggctttt catcaggaat atcaacatct cctgccaggg tgtcgttcgg
     1861 gctggtgttt tgtgcttttt cggccaaatc cgacaggttg tccgggatct cctca
//
LOCUS       DAOSFP010000416         1915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_60532_length_1915_cov_2.897849, whole genome shotgun sequence.
ACCESSION   DAOSFP010000416 DAOSFP010000000
VERSION     DAOSFP010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60532_length_1915_cov_2.897849"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLI16_10135"
     CDS             complement(<1..182)
                     /locus_tag="PLI16_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOH84958.1"
                     /translation="MDPEKLLTDVCSIARTAGSFLKSEIHKISEKNIETKSEHDFVTY
                     VDKASEKFLIEELSTL"
     gene            358..1404
                     /locus_tag="PLI16_10140"
     CDS             358..1404
                     /locus_tag="PLI16_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH84959.1"
                     /translation="MKKLFLYLTFFVFAFTVAAQTRLTFMTNAPRNGDAPKYYAFDYV
                     DPGVAGGGQNWDFAKIVFNGKEIISNVGIEPAAPLMDAVNYNSVTGEDGYEYYNKINE
                     NGVELKGYTTKDISLIYTDPITKMKYPFAYGDSYVDFFAGEAHYLIVRGITFEGKYQV
                     SADAYGTLRMPDKTIKNALRVKVVKTAVEYNPCSTIDLTHTLYYWFADGYRYPLVMMS
                     HKEYQRSYEKVPNIETLAYYSPQEAQTAEENALGTMEYTAADFALNTYPNPFAGMTSC
                     TYFLRKSMDVTAELYDITGKTIAVMVNNQKQEEGVHSLDIDGAELGLVPGVYYIKFTF
                     DKKEVVQKIVKIQS"
     gene            complement(1401..1901)
                     /locus_tag="PLI16_10145"
     CDS             complement(1401..1901)
                     /locus_tag="PLI16_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84960.1"
                     /translation="MKSRRSTYNLLFMATCVVFLFCSCVQPPADSEKPAPTTPARDST
                     IEKYLDTQVMQPGFGGRIFSAYKVFSEEDDKVYLWAFVQEYYEKDGKTLMGSGWSVPV
                     VLEIERNTSEITIKGHKTPRDGEQYAQDIKKMFPKNIRTQIFTFPGSDEMQELQRYSA
                     ARAKKP"
BASE COUNT          540 a          398 c          407 g          570 t
ORIGIN      
        1 aagagggttg aaagttcttc aatcaaaaac ttttcggaag ccttgtccac ataggtaaca
       61 aaatcatgtt ccgacttggt ttcaatgttt ttttccgata ttttatgtat ttccgacttg
      121 agaaatgatc cggcagtgcg ggctattgaa cacacatcgg tcagtaattt ttcaggatcc
      181 atgagtatgt ttttttgtcc gttaaagata cagaaaaata atatctgtaa ataaaagtat
      241 ttggcaattt tttcattgag cctgacgggc atttataaca cttccctgtt tgtaatattc
      301 caaccgcaac ttgccaatta caaaaaatat ttgtattttt gtacaaaact caaacttatg
      361 aaaaagctgt ttttatacct gacatttttt gtatttgctt tcactgttgc cgcccagaca
      421 cggcttactt ttatgactaa tgcaccccgt aatggcgatg ccccgaaata ctacgctttt
      481 gattatgtag atcctggtgt ggcaggcggc gggcagaact gggactttgc gaaaattgtt
      541 tttaacggta aggaaatcat ttctaatgta ggcatagagc ctgctgctcc gctgatggat
      601 gccgtcaact ataattcagt taccggggaa gacggttacg aatattataa caaaatcaat
      661 gaaaacgggg ttgaattaaa gggttatacc accaaagaca tttcgttgat ttatactgat
      721 cctattacca aaatgaagta tccttttgct tatggcgaca gttatgttga tttttttgcc
      781 ggcgaagccc attatctgat tgtgcggggt ataacgtttg agggcaaata tcaggtgagt
      841 gccgacgcct atggaacgct gaggatgccc gacaaaacca taaagaatgc cctgcgtgtt
      901 aaggtggtga aaaccgctgt tgaatacaac ccatgctcca ccatcgacct gacacatact
      961 ttgtattact ggtttgccga tggatacagg tacccgctgg tgatgatgag ccacaaagag
     1021 tatcagcgaa gctatgagaa agtccccaat atcgaaacgc tggcttatta cagcccgcag
     1081 gaagcccaaa cggctgaaga gaatgccctt ggaactatgg agtatactgc cgccgatttt
     1141 gctttgaata cctatcccaa tccttttgca gggatgacca gttgcactta ttttttgaga
     1201 aagtccatgg atgttaccgc ggaattatat gatatcaccg gaaaaactat tgcagtgatg
     1261 gtaaacaatc aaaaacagga ggaaggtgtc cacagcctcg atattgatgg tgctgaactg
     1321 ggcctggtgc caggagttta ttacatcaaa ttcacttttg ataaaaagga agtagtacag
     1381 aaaatcgtaa aaatccagtc ttaaggtttt ttcgccctcg cagccgaata ccgctgtagc
     1441 tcctgcattt catcagaacc ggggaaagtg aagatttgcg tccggatatt tttaggaaac
     1501 atttttttga tgtcctgtgc atattgttct ccatcgcggg gagttttgtg gcctttgata
     1561 gtaatctcac tggtatttct ttcaatttcc agcacaacag gtaccgacca tccgctgccc
     1621 atcagggttt tgccgtcttt ttcataatat tcctgcacaa aagcccacag gtacacttta
     1681 tcatcctcct ccgaaaaaac tttgtaggcg ctgaatatcc tgccaccaaa gcctggctgc
     1741 attacttgtg tgtcaaggta tttttcaata gtactgtccc gggctggtgt ggtcggtgct
     1801 ggcttttcag aatcagccgg tggctgcaca caagaacaaa ataaaaatac cacacaggtc
     1861 gccataaaca acaaattata agtgctccgc ctgcttttca taatttagtt tttga
//
LOCUS       DAOSFP010000417         1906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_60917_length_1906_cov_4.880065, whole genome shotgun sequence.
ACCESSION   DAOSFP010000417 DAOSFP010000000
VERSION     DAOSFP010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60917_length_1906_cov_4.880065"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1621
                     /locus_tag="PLI16_10150"
     CDS             <1..1621
                     /locus_tag="PLI16_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84961.1"
                     /translation="NEAESAGNEIEKTDKKTAASKALAVHPAIRYSLYVLVLAISFVF
                     FTQKIVFINADLGRHIKNGEVIVNTGKIIPANYYSYTEPDFPVVYHHWGSGVVFYLIQ
                     KIMGFAGLSIIYCLLSALAVFLLYKTAENKSHPYVAFLFFILCIPLIAMRTEIRPEVF
                     SLLFMAIYIYLFELFISSKIHIRTLLITLPLLQLLWVNLHIFFVFGLIIAGVYIAYIW
                     WLRKNKETNKWLLLLSGLLIGVCFINPYGLQGVLTPLTIFNNYAYMVAENQSVFFMQN
                     RFGNGVYIHYEVVTLLTVLLMLWYLKKSGFKPGTVWFVLVLLLLFVASGYKAIRLIPF
                     AGAMLLAFAPGIFMPLTANSGAGFKKSLTILSLIAAIVLTMVYFAKKDTYLSLKKSGR
                     GFGIYKGINNSADFFKANHFKGPVFNNYDIGGYLIYHLFPENRVFIDNRPEAYSTDFF
                     EKVYNPMLEKETVWQQFDKKYQFNCIYFFRLDETPFGQPFIIKRINDRGTWAPVYVDD
                     AAIILLKRNARNQSLIQQYELPPETFVVTKN"
BASE COUNT          542 a          403 c          383 g          578 t
ORIGIN      
        1 aaatgaagca gaatcagctg gtaatgagat agaaaaaaca gataaaaaaa cagcggcttc
       61 taaagctctg gccgtccatc cggctattcg ttattcatta tatgttctgg tgctggcaat
      121 tagttttgtg ttttttacac aaaaaattgt atttatcaat gccgacctgg gaagacacat
      181 caaaaacgga gaagtaattg taaacaccgg caaaattatt cctgccaatt attactctta
      241 caccgagcct gattttccgg ttgtctatca tcactggggt agcggcgttg tgttttatct
      301 gattcagaaa attatggggt ttgcaggctt atctataatt tattgcctgc tatcggcttt
      361 agccgtattc cttttgtaca aaaccgccga aaacaagagc catccttatg tggccttcct
      421 gttttttatc ctgtgtatcc cgctgattgc catgcgcacc gaaataaggc ccgaggtctt
      481 cagcctgctc tttatggcca tttacattta tttgtttgaa cttttcatat cctcaaaaat
      541 tcacatcaga acactattga tcaccttgcc actgctacag ttgttatggg tcaacctgca
      601 catctttttt gtatttggat taatcatcgc cggcgtttat attgcctata tctggtggct
      661 ccgcaaaaat aaagaaacaa acaagtggct gctattactg tcaggactcc tcataggcgt
      721 atgttttatc aacccttacg gacttcaggg cgtgttaacc ccgctgacaa tctttaacaa
      781 ctacgcctat atggtggccg aaaaccagag tgtgtttttc atgcaaaacc gttttggcaa
      841 tggtgtatat atccattatg aagtggtaac actcctcacg gtgctgctga tgctatggta
      901 cctgaaaaaa tcaggattca aacccggtac ggtgtggttt gttcttgtgt tactgctgct
      961 ttttgtggca tccggatata aggcaatccg cctgatacct tttgcaggag ccatgctgct
     1021 tgcttttgct cccggtattt ttatgccact gacagccaat tcaggtgcag gattcaaaaa
     1081 aagcctgaca atactaagtc taatagcagc catcgtattg acaatggtgt attttgcaaa
     1141 aaaagacacc tacctgtcgt tgaaaaagtc aggccgagga tttgggatat ataaagggat
     1201 aaacaactca gccgattttt tcaaagcaaa ccatttcaaa ggccctgttt ttaacaatta
     1261 cgatattggt ggctacctga tataccatct gtttcccgaa aaccgtgttt ttatcgataa
     1321 ccggccggag gcatactcca ccgatttctt tgagaaagta tacaatccca tgttagaaaa
     1381 agagaccgta tggcaacagt tcgataagaa ataccaattc aactgcatat actttttcag
     1441 gttagacgag acaccctttg gacagccatt tatcattaaa aggatcaacg accgtggcac
     1501 gtgggcgccg gtatatgtag atgacgccgc tatcatactt ttaaaaagaa atgcccgtaa
     1561 ccaatccctc atacagcagt atgaattacc tcccgaaacc tttgtggtga cgaaaaattg
     1621 aaatccggaa gggagtaagt ggcataatag gccaccacgt tcctcttgca caactcgggg
     1681 gttatcgaaa aatatatttt ctcatttttt aattcattca aaacattata ctacttttga
     1741 tttcttatgg tatgacattt atgtaagcct acggcaaagt acatgttttt tttaaaccag
     1801 caagggttta attttgcaca ggcagaagtt cgtgcaacat gtttgcaggg caaacaggca
     1861 ggatagtaag gatttctttg agccacgcgt ggggttcgac attatt
//
LOCUS       DAOSFP010000418         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_61193_length_1901_cov_3.854821, whole genome shotgun sequence.
ACCESSION   DAOSFP010000418 DAOSFP010000000
VERSION     DAOSFP010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61193_length_1901_cov_3.854821"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1550
                     /locus_tag="PLI16_10155"
     CDS             <1..1550
                     /locus_tag="PLI16_10155"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOH84962.1"
                     /translation="FGGDTDNWMWPRHTGDFSVFRIYADKNNQPAKYSQDNVPYKPPF
                     HFPVSLKGVKPGDFTFIFGYPGTTNEYLVSYAVDRMVNYENPAAIKARGKRMEVYNKY
                     MAQSKKTRIQYSSKLASVENYHKKMIGESKGVVRTNIIAKKQELEKQFIQWAQASPEK
                     QNKYNGLIEKFEQLYKNYGANNLAADYVFEAGLGMEIVRYAANYEALVKSSQDKKQND
                     DAINKTLERIKLASEGFFKNYDSRIDHEVVGLLMEIYCSAPDKDLMPAEVARVGAAYK
                     GDFKKYADDLFAQSFMTDEQKVNDFLKNYKPSKAKEIEKDPAYILAMSIYDLYRKTIY
                     PKYVEYQDKADELYRVYMQGLMEMQPEKKFYPDANMTLRVAYGQVGGFNPRDAVRYEY
                     FTTLKGVIEKEDSTIYDYTVNSKLKDLYNSKDYGMYADTDGSMHVAFVATNHTTGGNS
                     GSPVLNADGHLIGINFDRVWEGTMSDIVFEPAMCRNISVDIRYCLFIIDKYAGAHRLI
                     EEMTLVK"
     gene            1618..>1901
                     /locus_tag="PLI16_10160"
     CDS             1618..>1901
                     /locus_tag="PLI16_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOH84963.1"
                     /translation="MKKIYRFVLKSFIGPMALTFFIALFILLMQFLWKYVDDMVGKGF
                     EWYVLAELMFYASATFVPMALPLAVLLASLMTFGNLGEHYELVAIKAAGIS"
BASE COUNT          582 a          389 c          452 g          478 t
ORIGIN      
        1 agtttggcgg cgatacagat aactggatgt ggccgcgtca taccggtgat ttttcagtgt
       61 tccgcattta cgccgataaa aataaccagc cggcaaaata ttctcaggac aacgtgcctt
      121 acaaaccgcc gtttcatttt ccggtatcct taaaaggtgt gaaaccgggc gattttacct
      181 ttattttcgg gtaccccggc actaccaacg aataccttgt ttcctatgcg gtggacagga
      241 tggtgaatta cgaaaacccg gctgctatta aggccagagg aaaacggatg gaagtgtaca
      301 acaagtatat ggcccaaagc aaaaaaacac gcatacaata ttcgtcgaaa ctggccagcg
      361 tggaaaacta ccataaaaaa atgatagggg agagcaaagg agtagtcaga accaatatta
      421 ttgccaaaaa acaggagctg gaaaagcagt ttatccaatg ggctcaggcc agccctgaaa
      481 aacaaaataa gtataacggc cttattgaga agtttgaaca gttgtacaaa aactacggcg
      541 ccaataacct ggctgccgat tatgtatttg aagccggtct gggaatggaa atcgtgcgtt
      601 atgcggccaa ctatgaggca ttggtaaaaa gcagccagga caagaaacag aacgatgatg
      661 ccatcaataa aaccctggag cggattaaac ttgcctccga aggatttttc aaaaattacg
      721 acagccgcat cgatcacgaa gtggtgggtt tgctgatgga aatctattgc tcagccccgg
      781 acaaggattt gatgcctgcc gaagttgcac gggtcggagc tgcatacaaa ggcgatttta
      841 aaaaatatgc cgacgacctc tttgcccaat cttttatgac ggacgaacaa aaggttaacg
      901 atttcctgaa aaattataaa ccatccaaag ccaaagaaat agaaaaagat cctgcttata
      961 tactggcgat gagcatttac gacctgtaca gaaaaacaat ttacccgaaa tatgtggaat
     1021 atcaggacaa ggccgatgaa ctttaccggg tatatatgca gggattgatg gaaatgcaac
     1081 ccgaaaaaaa gttctatccc gacgccaata tgaccttgcg tgttgcttat ggccaggtgg
     1141 gcggcttcaa tccccgcgat gctgtcaggt atgaatattt taccaccctg aaaggtgtca
     1201 tcgaaaaaga agactccacc atttatgatt ataccgtaaa cagcaaactc aaggatttat
     1261 acaatagcaa ggattacggc atgtacgccg acactgacgg gagtatgcac gtggcttttg
     1321 tcgccaccaa ccataccacc ggcggcaatt cgggaagtcc ggtattaaac gccgacggac
     1381 acctgatagg tatcaatttc gacagggtgt gggaaggcac catgagcgac attgtctttg
     1441 aaccggctat gtgcaggaat atctcggtgg atataagata ctgccttttt atcattgata
     1501 aatatgcagg agcacaccgg cttattgagg aaatgacgct ggtaaaataa attattgttc
     1561 aaacttaccg agtcaactga tattaaggta tagaaactat ttttcaataa acaagtgatg
     1621 aagaagatat accggtttgt gctgaaatcc tttatagggc cgatggcgct gacttttttt
     1681 attgccttgt ttatcctgct gatgcagttt ttgtggaagt atgtggacga catggtgggc
     1741 aaaggatttg aatggtatgt cctggccgaa ttgatgtttt atgcttcagc tacttttgtg
     1801 cctatggctt tgccgctggc cgtgttattg gcatcattga tgactttcgg caacctgggt
     1861 gagcattatg aactggtggc tataaaagct gccggcatat c
//
LOCUS       DAOSFP010000419         1896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_61424_length_1896_cov_3.918523, whole genome shotgun sequence.
ACCESSION   DAOSFP010000419 DAOSFP010000000
VERSION     DAOSFP010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61424_length_1896_cov_3.918523"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLI16_10165"
     CDS             complement(<1..332)
                     /locus_tag="PLI16_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84964.1"
                     /translation="MKKGYFIIISALLLTFFSSAQNVEFVKSNFKGREKEFKQARNNF
                     TVGLKYYEMGKSMYATALEYFLEAQKFNPDNAKLNYLIGTCYLNTVQKTKSIEYFEKS
                     LLLNAVEY"
     gene            complement(360..1409)
                     /locus_tag="PLI16_10170"
     CDS             complement(360..1409)
                     /locus_tag="PLI16_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH84965.1"
                     /translation="MKKSVFILCFLLLVFTLSAQEVSTDTLFKGDYQDAEKVYNEGVK
                     FFEHKKYNEAIKKFSEAINLKPDFEKAYFNRGAVNMEQNKFQEAIGDFSKSIELAPTA
                     NGYFSRGKCRFELKDHDNAMTDFTQAVALDANHAQSYYYRGEIFFEKNEYEKAISDFD
                     AAIKNKPDYAYAYNDRGSAKVKLDKFEEAKADYIIAAQINPNMPFTYNNLGSAKKKLK
                     DYTGAIADYSTAIQKKPDYFIAYNNRGTAHFELEEYEDAIRDFDQAIKLNPTYYYAYN
                     NRGNAKYKLQDYEGAIADYTKAIELNPSYGYAFLNRGIAYEMLRKPQNSCEDWKKAAL
                     LGITTAASYSNLQCK"
     gene            complement(1491..>1896)
                     /locus_tag="PLI16_10175"
     CDS             complement(1491..>1896)
                     /locus_tag="PLI16_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HOH84966.1"
                     /translation="ALTAHFHYLPLLYSLAVLCWVSGFDIIYALSDMNFDKQHGLHSI
                     PERFCLTSSLVISLLLHIVCLMVIIIAGIANHQGIFFWTGAGIFAFLLFYQHYIVKPN
                     DLSRINFAFFTLNGISGLVLLFFFLLDFFLAT"
BASE COUNT          513 a          351 c          430 g          602 t
ORIGIN      
        1 ttgtattcaa cagcgtttaa cagaagtgat ttttcaaaat actctattga tttagttttt
       61 tggactgtat tcaggtaaca tgtacctatc aggtaattca gcttggcatt gtcggggtta
      121 aatttttgtg cctccaaaaa atattcaagg gcggtggcat acatgctttt gcccatttca
      181 taatacttca gtccgacggt gaaattgttt ctggcctgtt taaattcttt ttctctgccc
      241 ttgaaattgc ttttaacaaa ttcaacattt tgggcagacg aaaaaaaagt gagcaataat
      301 gcagagatta ttatgaaata tccttttttc atggtgatat tatttgaatt ataaaactat
      361 tatttgcact gtagatttga ataactggca gcggtggtga tgcccaataa ggcggctttt
      421 ttccagtctt cgcaggaatt ttggggtttt cttagcattt cgtaagcgat gccgcggttc
      481 aggaacgcgt agccatagct gggattgagt tcaatggctt tggtgtagtc ggcaatggca
      541 ccttcgtaat cctgcagttt gtattttgcg ttaccccggt tgttgtatgc ataataatac
      601 gtggggttga gctttatggc ctggtcaaaa tccctgatgg cgtcttcata ttcttccagc
      661 tcaaaatgtg cggtaccccg gttgttataa gctataaaat agtcgggttt tttttgtatg
      721 gcagtggaat aatcggcgat agcgcctgta taatccttta atttcttttt tgcgctgccg
      781 aggttattgt atgtaaaagg catattggga tttatttgtg cagctattat ataatcggct
      841 ttggcttctt cgaatttgtc gagtttcact ttggcgctgc ccctgtcatt atatgcgtag
      901 gcgtaatcag gtttgttttt tatggccgca tcgaaatcgc tgatggcttt ttcgtattca
      961 tttttttcaa aaaatatttc cccgcggtag tagtacgatt gtgcatgatt ggcatccagg
     1021 gccaccgcct gagtaaaatc ggtcatggcg ttgtcatggt ctttgagttc gaaacggcat
     1081 tttcccctgc tgaaatatcc gttggcggtg ggtgccagtt ctatggattt tgaaaagtcg
     1141 ccgatggcct cctgaaattt gttctgttcc atatttacgg cgcccctgtt gaaataagct
     1201 ttttcaaaat caggtttcag gttgatagct tcggagaatt ttttgatggc ttcgttgtac
     1261 ttcttgtgtt caaaaaattt cactccttcg ttatacacct tctcggcgtc ctggtagtcg
     1321 cctttaaaca aagtatcagt ggaaacttcc tgggcgctga gcgtaaatac cagcaagaga
     1381 aagcacagga taaaaacaga ctttttcata aataaatttt tataatgaat gtacgtgtta
     1441 atgattgcaa atataacaat tgatagcaga taaaaaacaa tgtgagggtt ttatgttgct
     1501 agaaaaaaat ccagaaggaa gaaaaacagc aataccaggc cggaaatccc gttgagggtg
     1561 aagaaggcaa aatttatccg gctcaaatcg ttcggcttta caatgtagtg ttgataaaaa
     1621 agcaggaagg caaaaatacc tgcgcctgtc caaaaaaata tgccctggtg attagctatt
     1681 ccggcaatga tgataaccat caggcaaaca atatgcagca gcagcgagat gactaaagat
     1741 gatgtcaggc aaaaccgttc ggggatagaa tgcagcccat gttgtttgtc gaaattcatg
     1801 tcgctcaacg catagatgat gtcgaacccg ctgacccagc acagtacagc caaagaatac
     1861 agcagcggaa ggtagtggaa atgtgccgtc agggcc
//
LOCUS       DAOSFP010000420         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_61636_length_1892_cov_3.644529, whole genome shotgun sequence.
ACCESSION   DAOSFP010000420 DAOSFP010000000
VERSION     DAOSFP010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61636_length_1892_cov_3.644529"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1554)
                     /locus_tag="PLI16_10180"
     CDS             complement(85..1554)
                     /locus_tag="PLI16_10180"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOH84967.1"
                     /translation="MKKVLFFISIILLTAVSFSAQAKRLQAYMSYSTFYSPADGPYIE
                     TYMIVVGNSLKYVKNQNGKYQGTIEITMIFRQDSLIKDFKKYNLLSPETEDTNTINFH
                     FIDQQRFLLPNGSYQVEIIIADKNDNAPPFKSYETIVIEFPADKITLSGIELIDSYKK
                     TDSVNMLTKNGFELMPYIIYYYPEKVGKLAFYAEIYNTGKVLGENEKFLLTSYIEVNE
                     NNNLYGEFKKIKKETARDVVVVLSEFDLALLPTGNYNLVIEVRDKQNNLQAIQKLYFE
                     RDNTKVAFENKIYENAVTEHTFVEMYNNKDTLAAYIRCLYPISSESERMYAKNQINHN
                     DLKSMQKFFLTFWEARDTENPGKAWADYLLEVQKVNHDFSTKIRKGYETDRGRIYLQY
                     GPPNSISKSYYEPSAYPYEIWHYYTLKNQKNKKFVFYNPDLVTNDFTLIHSDATGEIS
                     DYRWEQKIYGRNNPTNDLDNSGVRNHWGRKSDEYFKNPY"
     gene            complement(1755..1839)
                     /locus_tag="PLI16_10185"
     tRNA            complement(1755..1839)
                     /locus_tag="PLI16_10185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1805..1807),aa:Ser,seq:tga)
BASE COUNT          508 a          330 c          395 g          659 t
ORIGIN      
        1 atatattgat atttatggta agggaaaaat ataaactagc atattcttaa aaatgctcct
       61 tggtattata tataattagg tgtcttaata aggatttttg aaatattcat cgctttttct
      121 gccccagtga tttctgacac cgctgttgtc aaggtcgtta gtgggattgt tgcgtccgta
      181 aattttttgc tcccacctgt agtcgctgat ttcgccggta gcatccgaat gtatgagcgt
      241 aaagtcgttg gttactaaat cgggattata aaacacaaat tttttgtttt tctgattttt
      301 taaggtgtag taatgccaaa tttcataagg gtaggcactg ggctcgtagt agctttttga
      361 tatcgaattg ggggggccat actgcagata tatccggccg cggtcggttt catatccttt
      421 acggattttg gtgctgaaat cgtgattcac tttttggact tctaataagt aatcagccca
      481 ggcttttccc ggattttctg tatctcgcgc ttcccagaaa gtaaggaaaa acttttgcat
      541 ggatttcagg tcgttgtggt tgatttggtt tttagcatac atgcgttcgc tttcggacga
      601 aatagggtag agacagcgga tataggccgc cagcgtgtct ttattgttgt acatttcaac
      661 aaaagtatgt tctgtaacag cattttcata aattttgttt tcaaaagcta cttttgtgtt
      721 gtcgcgttca aaatatagtt tttgtatcgc ctgaagattg ttttgtttat cgcgtacttc
      781 aatcaccagg ttgtagttgc cggtgggcag aagcgccagg tcaaactcac tcagcacaac
      841 gaccacatcc cgcgcggttt ctttttttat ctttttaaat tctccgtaaa gattgttgtt
      901 ctcattgact tcgatatacg atgtgagtag aaacttttcg ttttcgccca ataccttccc
      961 ggtattgtat atctcagcat agaatgccag tttgccaacc ttttcaggat aataatagat
     1021 gatatagggc atgagttcga acccgttttt ggtaagcata tttaccgaat cagttttttt
     1081 ataggagtct ataagttcaa tgcctgataa ggtaatctta tcggcaggaa attctatcac
     1141 gatagtttca tagcttttaa atggcggagc attgtcgttt ttatcagcaa tgattatttc
     1201 cacctgatat gagccgtttg gcaacaggaa ccgttgctga tcgataaaat gaaaatttat
     1261 tgtattggta tcttcagttt ctgggcttag caggttatat tttttaaaat ccttgatcag
     1321 actgtcttgc ctgaaaatca ttgtaatttc gatagtgccc tgatacttgc cgttttgatt
     1381 ttttacatat ttcaggctgt tgcccaccac tatcatgtat gtttcgatgt aggggccatc
     1441 ggcaggtgaa taaaatgtgg aatacgacat ataggcctga aggcgttttg cctgggccga
     1501 aaagctcaca gccgtcagga ggatgatgct gataaaaaat aatacttttt tcattttttg
     1561 cataatatat atgcaaagat aagtcatttt ttggcggaaa ggaaatttta ccccggtgat
     1621 ttgtggatta atcagtttgc ggtatggttt ttcaagaaaa tggagtgaaa tattctttgt
     1681 ggactaatga tagataaaat agttaaataa aaaaccgtaa attattgatt attaataatt
     1741 tacggttttt tctgcggaga gagagggatt cgaacccccg gacccattgc tggatcaacg
     1801 gttttcaaga ccgccgcaat cgaccactct gccatctcct gacttcgaca caataaaaaa
     1861 attagactta taacatactg attatattgt gt
//
LOCUS       DAOSFP010000421         1852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_63715_length_1852_cov_4.538119, whole genome shotgun sequence.
ACCESSION   DAOSFP010000421 DAOSFP010000000
VERSION     DAOSFP010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63715_length_1852_cov_4.538119"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLI16_10190"
     CDS             complement(<1..508)
                     /locus_tag="PLI16_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH84968.1"
                     /translation="MEKTKKIVMITGATSGIGLACAEIFAMHGYDLIITGRRNERLLK
                     ISKSFPEKYNVSVLPLNFDVRNKNETEKALGQLPEKWKNIDILINNAGLARGFNLIQD
                     GDTQDWDEMIDTNLKGLLYVSKFVIPLMIARQKGHIINVGSIAGKEPYINGNVYCATK
                     AAVDSLTRS"
     gene            complement(820..>1852)
                     /locus_tag="PLI16_10195"
     CDS             complement(820..>1852)
                     /locus_tag="PLI16_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84969.1"
                     /translation="DSPYFDQYYYQMNNWYRQYESNIYNDSHTIIRINALIRLFSFGF
                     YNVHTVFMCFISLVGLVALYKFFVPYMQHKKRLLFLAVFLIPSVVFWSSGVLKEAILI
                     FGIGMMLFSTSQLLQKKHFLLNTLFLVFSLVLLMYIKYYIFMIIIPLTIIHVWCRLTS
                     DKLAALKYASMAVVCIIAGLTIHYIFPGLNMLQILAMKQNDFVGLAQATHSGSLISED
                     LLQPTVSDVLIHTPGAFYNTLVRPYIFEANSLIILFAAIENLFIFAVIIVCLIFSSGK
                     IAHKSVFIFCLLFFTALYILTGLTTPVIGAMVRYKVPAMPFLMILLFMLLNSRKVAEK
                     FPSLKKLID"
BASE COUNT          565 a          369 c          412 g          506 t
ORIGIN      
        1 ttgacctggt cagtgaatcc acggcagctt tggtggcgca atatacattt ccgttgatat
       61 acggttcttt accggcaata gatcctacgt tgataatatg tcctttttgg cgtgctatca
      121 tcaaaggaat tacaaatttc gaaacatata acagtccctt gaggttggtg tcaatcattt
      181 cgtcccaatc ctgcgtatct ccgtcctgaa tcaggttaaa cccccgtgcc aaaccggcat
      241 tgtttatcag gatgtcaata tttttccatt tttcgggaag ctggccaagt gccttttcgg
      301 tttcattttt gttgcggaca tcaaaattaa gaggcaggac actgacgtta tatttttcag
      361 gaaaactttt gctgatcttt agcaagcgtt cattacgccg tccggtgatg atgaggtcgt
      421 aaccgtgcat ggcgaaaatt tcggcacagg caaggcctat tccactggta gcgccggtga
      481 tcattacaat tttttttgtc ttttccattg gataggattt attaatgctt attagtaact
      541 gttttgaatt tgtataatta gtcattattt acggtcaggc tgagccgatt attctcatcc
      601 gatagatcct acctgtaggc aaaacaggga ggataatcgt gtccctgcct gccgaagtgt
      661 ccggcacgca ggcagggaag tcgaccgtag cagccactct tcgacactga caaatacttc
      721 aacaaaagta tttgtccggc tctgagtaac tttagagaat aaaaatttca ttttaatgat
      781 ttattttata aaaatcaaaa cagcttcata aagtatgggt caatcaatta attttttcaa
      841 tgatggaaac ttttctgcaa ccttgcggct gttcaacagc atgaaaagta aaatcatcag
      901 aaatggcatg gccggaacct tgtaacgtac catggcaccg ataaccggcg tggtaagacc
      961 agtgagaata tacaaggcgg taaaaaacag caaacaaaaa ataaatactg atttgtgggc
     1021 aatttttcct gaggaaaaaa tcaggcagac gattatcacc gcaaaaataa acaggttttc
     1081 gatggcggca aaaagtatta tcagcgagtt ggcttcaaaa atataaggcc tgaccagggt
     1141 gttatagaat gcaccggggg tgtgtatgag gacatcggac accgtaggct gtagcaaatc
     1201 ctcactgatg aggctgcccg aatgggtggc ctgcgccagt ccgacaaagt cgttttgttt
     1261 cattgcgagg atctgaagca tattcagtcc ggggaaaata taatggatcg tcagtccggc
     1321 aataatgcat actactgcca tggaggcgta cttcagcgca gctaacttgt cggaggtcag
     1381 ccggcaccat acatgaatta tggtcagcgg tatgatgatc atgaatatgt agtatttgat
     1441 atacatgagc agcaccaacg aaaacaccag gaacaatgtg ttcagcaaaa aatgtttttt
     1501 ttgaagcaac tgactggtcg aaaaaagcat catgccgata ccaaaaatca aaatggcttc
     1561 cttgagcact cctgaactcc aaaaaacaac tgaaggaatc agaaatacag ccagaaacaa
     1621 caagcgtttt ttgtgttgca tgtaaggaac aaaaaatttg tacaaagcta ccaggccgac
     1681 caatgaaata aaacacataa aaaccgtgtg gacattgtaa aaaccaaagg agaacagacg
     1741 aatcagggcg ttgatgcgga taatcgtatg gctgtcgttg tatatattgc tttcgtattg
     1801 ccggtaccag ttgttcatct ggtagtaata ctggtcgaag tacggagaat cg
//
LOCUS       DAOSFP010000422         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_63770_length_1851_cov_3.911470, whole genome shotgun sequence.
ACCESSION   DAOSFP010000422 DAOSFP010000000
VERSION     DAOSFP010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63770_length_1851_cov_3.911470"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..637)
                     /locus_tag="PLI16_10200"
     CDS             complement(302..637)
                     /locus_tag="PLI16_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84970.1"
                     /translation="MEKVLSHGKNFGNENLKTYELMFRFSGHEKKVTQDDIETIKYRF
                     EAVVKPIQISSYLTRKITLNELGGSVICYVVYENSVYLISADDWTIIRNDSDSLAAQL
                     ENLLTPLLG"
     gene            complement(991..1410)
                     /locus_tag="PLI16_10205"
     CDS             complement(991..1410)
                     /locus_tag="PLI16_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOH84971.1"
                     /translation="MEQIKSDKVSINAPVEKIFNFLSDFNNFQTMMPEQAKNWTSTAD
                     TCSFEVSGMGTLALKITEKVPYTKILIVPDTGTKLPFTFELICLLDKIGDNQTEVVFV
                     FEHEMPMMIRMMAARPLQNLVNILAGKLKEYCESPAF"
     gene            complement(1417..>1851)
                     /locus_tag="PLI16_10210"
     CDS             complement(1417..>1851)
                     /locus_tag="PLI16_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOH84972.1"
                     /translation="SPDVIAGVATGAIAHGALVAQDLGLPFVYVRSAPKDHGLTNLIE
                     GVVENGQSVVVVEDLISTGVSSLKAVEALRQAGCDVKGMVAIFTYGFPEAEENFEKAK
                     CKLFTLSNYYALLEQAVESSFIKESDLPYLTQWREAPDKWGK"
BASE COUNT          552 a          396 c          355 g          548 t
ORIGIN      
        1 accgtttcgg aaagcacagt atgtttgaat ttgacaaaaa aacaatccgg gctgttattt
       61 gcggggtgga tgaatatgga tggctgcagt tacttaccga tgaaggtgtt gtactaagcg
      121 gcgaaatgga caaggtaaaa atgataattg agccgtgagt ctaagcccta tagatggaaa
      181 attggaatat taaggtaatg aacagtgaat cattatccca taatcccaaa aaattctccc
      241 agtaaaaaaa ttagtttcat tctgccctca attgccttta aggcattgac agccctatta
      301 gctaccccaa taatggtgtc agcaggttct cgagttgtgc agccagcgaa tcggaatcat
      361 ttctaatgat ggtccagtca tccgcgctga taagatatac gctgttttcg tacaccacat
      421 aacaaattac cgagccacca agctcattca aagtgatctt acgcgtcagg taactggata
      481 tttgaatggg ttttacaact gcttcgaacc ggtatttaat ggtttctata tcatcttgag
      541 taactttttt ttcatggccc gaaaacctaa acatcagctc ataagttttc aggttttcgt
      601 taccaaagtt ttttccgtga gaaagtactt tttccatatc agtaattatt tatttcattt
      661 taaattgatt tccggtatga aattacaaca aattcctgac actataacct gatttatcaa
      721 tattccgctc attaaaattc atccatacaa agccggttta cacctgcaat aacaaggaaa
      781 aaatgcaatg gttaactatt aaactattac tttaaatata aagtgatatc tatctgtatt
      841 aatgctcctt aatgtatcga cattatttca ttaaaaccat atagattttt ttccggcagg
      901 tggcggacaa gatggtgttt gactgaacat cgacttctga ttgaatccca ccaataaaaa
      961 aaaggttcaa caaaaaaaac ttatgaaaaa ttaaaacgca ggactttcgc aatattcctt
     1021 cagttttcct gcaagaatgt taaccaggtt ttgcaaaggt ctggcggcca tcatccttat
     1081 catcatgggc atctcgtgct caaaaacaaa aaccacctct gtttggttat cacctatctt
     1141 atcgagcaga cagatcagct caaatgtaaa cggcaattta gtgcctgtat ccggcacaat
     1201 aaggattttg gtataaggta ctttttcggt gattttcaga gcaagggttc ccatgccgct
     1261 gacctcaaaa ctgcatgtat ctgcggtgga cgtccagttt ttcgcctgct cgggcatcat
     1321 agtctgaaaa ttgttaaagt ccgaaagaaa attaaaaatt ttctcaacag gggcattgat
     1381 gcttacctta tcgcttttaa tctgctccat agtgcactat tttccccatt tatcaggtgc
     1441 ttcgcgccac tgtgtcaggt atgggaggtc cgactctttg ataaaactac tttctacagc
     1501 ttgttcaagc aaggcgtaat aattggataa ggtaaagagt ttgcattttg ctttttcaaa
     1561 attctcttct gcttcgggaa agccataagt aaaaatggcc accattcctt ttacatcgca
     1621 acccgcctgc cggagggctt ctactgcttt aaggctgctg acaccggtgg atataaggtc
     1681 ttcgaccact accaccgact gtccgttttc caccacgcct tcaataaggt tggtaaggcc
     1741 atggtctttg ggtgcagaac gcacatacac aaaaggcaag cccaggtcct gagccaccag
     1801 cgcaccatga gcaatggctc cggtagccac accggcaata acatcgggag a
//
LOCUS       DAOSFP010000423         1848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_63937_length_1848_cov_3.203012, whole genome shotgun sequence.
ACCESSION   DAOSFP010000423 DAOSFP010000000
VERSION     DAOSFP010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63937_length_1848_cov_3.203012"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            261..1517
                     /locus_tag="PLI16_10215"
     CDS             261..1517
                     /locus_tag="PLI16_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOH84973.1"
                     /translation="MQFSSLQLIDPLLRSLEQEGYTVPTPIQQEAIPIVLKGKDLLGC
                     AQTGTGKTAAYAVPILQLLHEAKNYDRKRKIKSLIVTPTRELAIQINDSFIAYGRHTG
                     LKSAVVFGGVNQNPQTTALKHGTDILVATPGRLLDLMNQGFISLRDVQIFVLDEADRM
                     LDMGFIHDVRKIIAALPQKRQTLFFSATMPPEIIKLSKSILNQPARVEVTPSASTVDI
                     VKQFVYFIDKGNKTSLLVDLLKDTHIKTALVFTRTKYGADKVVRVLAKNKIKAEAIHS
                     NKSQHARQKALTNFKEQNTRVLVATDIASRGIDVEAMECVINFEISNMPETYVHRIGR
                     TGRAGAGGTAISFCDAEEKKYLHDIEKLIGKKIPVIEGHPYPLVDLHPVKPPKQVRTL
                     PTNPQRNYSRQMPDKKKLSGNISKWG"
     gene            complement(1622..>1848)
                     /locus_tag="PLI16_10220"
     CDS             complement(1622..>1848)
                     /locus_tag="PLI16_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244666.1"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HOH84974.1"
                     /translation="TSTNPLISATTPGLEINKWRCIVANEATGKTTREGVFAGGDAVT
                     GAATVILSMGAGRAAAKAIDEYVKKQTQNN"
BASE COUNT          555 a          440 c          361 g          492 t
ORIGIN      
        1 tttgttgcgt aatttttttt aacaatattt ggtcatgtct tagaaaacag gcaggtaaat
       61 gagaaatatc aatacgtcct attggactaa ttgatatttc aatttggtat aatatcaccc
      121 caaataaata ttcaaaactt ttaatatatt gtagtgtcaa ttattagttg tatctttgca
      181 gctcgaaatt cactttccaa catttccctg ttacaataac caggttttgg ttagaaaagc
      241 tatcccacct taattttttt atgcaatttt catcactaca attaattgac cctttattaa
      301 ggtcattaga acaggaaggt tataccgttc ctacacctat tcaacaagaa gccataccca
      361 tagtattaaa aggaaaagac ctgctgggat gtgcgcaaac cggcacgggc aaaaccgctg
      421 cttatgctgt tcccatcctt caacttttgc atgaggcaaa aaactacgac agaaaaagaa
      481 aaatcaagag cctgattgtt acccccaccc gtgagcttgc aatacagata aacgacagtt
      541 ttatagctta cggtcgccat acgggactga aaagcgcagt agtttttggc ggagtgaatc
      601 aaaatccgca aaccacagcc ctgaaacacg gaacagacat cctggtggct actccgggaa
      661 gactgcttga cctgatgaac cagggtttta tctcattgcg cgatgtgcag atttttgtgc
      721 tcgatgaagc cgaccgtatg ctcgatatgg gctttattca tgatgtaaga aaaatcattg
      781 ccgccctgcc gcaaaaacgc cagacattgt ttttttccgc caccatgccg cctgaaatca
      841 tcaaactatc caaatccata ttaaaccaac ctgccagggt agaagtcact ccatcggctt
      901 caacagtgga tatagtcaaa cagttcgttt attttatcga caagggcaat aaaacctccc
      961 tgctggtaga tttgctgaaa gacacccata tcaaaacagc cctggttttt acccgcacca
     1021 aatacggtgc cgataaggtg gtgagggtgt tagccaaaaa caaaatcaag gccgaagcca
     1081 tccatagcaa caaaagccag catgcccggc agaaagcgct gaccaatttc aaggaacaaa
     1141 atacacgggt gctggtagcc acagatattg cctcgcgcgg catcgatgta gaagctatgg
     1201 aatgtgtcat caacttcgaa atttccaata tgcccgaaac ctatgttcac cgtatcggcc
     1261 gcacaggccg ggccggcgct ggaggcacag ctatttcttt ctgcgatgca gaagaaaaaa
     1321 aatacctgca tgacattgaa aaactgatcg gaaaaaaaat cccggtcatc gaagggcatc
     1381 cctatccgct ggtggacctc cacccggtaa agcctccgaa acaggtgcga accttgccaa
     1441 ccaatccaca acgcaactac tcccggcaaa tgcccgacaa aaaaaaattg tcaggaaaca
     1501 tttctaaatg gggttaaaaa ttacctggat taattcaaaa aaatattggg tgtcagttat
     1561 cagttcctgc taatatccga cacccaataa aatatttgct cccgtggagc ggctccgcgt
     1621 attaattatt ttgtgtttgt tttttaacgt actcatcaat tgcctttgca gcagctctcc
     1681 ctgctcccat agacaatatt accgtggcag ctccggttac ggcatcgcct cctgcaaaaa
     1741 cgccttctct tgtcgttttt ccggtggctt catttgccac tatacaacgc catttattta
     1801 tttctaatcc gggtgttgtg gccgatatca acggattggt agatgttc
//
LOCUS       DAOSFP010000424         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_64874_length_1830_cov_4.427042, whole genome shotgun sequence.
ACCESSION   DAOSFP010000424 DAOSFP010000000
VERSION     DAOSFP010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64874_length_1830_cov_4.427042"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="PLI16_10225"
     CDS             <1..1577
                     /locus_tag="PLI16_10225"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HOH84975.1"
                     /translation="ILLSELPLEKIQRRASESFGWMSGNAAINNRISFNGTLPGKQAV
                     IQPKLDISHPEDDYEKESGTVADTVMRMTEPLAEDKPISFSAGLKGKPLASRISPLIH
                     TSTGGASMEASSLSGLESRLNTSKNNGSPLPQEARSFMENRFGTDFGHVKIHTDTGAV
                     QMNKELNAQAFTHGNHIYFNSGMFDTASFSGKHLLAHELTHVVQQNGNHFSVQRETTP
                     EEKELREKAELLETEILADPAYAGLKSQSKSRVKTIIKKAQKKPLGEKIGQRNYYLIK
                     LKTAITTPFKGQDTGNEEYGCSLEADKENREEVEKFLKIEGKYWSNIGFENVEEEIVA
                     GGKRKVMREGQDGKIFYVDRSDAKNIRVLIKVKLNGNPDEIVKIKALEDAIEREASTT
                     GYTLDIEFVDKSGFDVFEFSVSFCEWANSGNWASGPVSLSHEVHHALGLDDRYDYIES
                     HAHNPMMNVPMRLVWFEVQMDKTMTTRDDYSKMSSSENPMLSEDVCSVAFGSKANRDK
                     CIEARKELDPPGIPGQ"
BASE COUNT          591 a          386 c          426 g          427 t
ORIGIN      
        1 caatactgtt gtcggaactc ccccttgaaa aaattcaacg gagggccagc gaatcttttg
       61 gctggatgag cggaaatgcc gcgataaaca acaggatatc ttttaatggc accctccccg
      121 gaaaacaagc tgtcattcag ccaaaacttg acatcagcca ccccgaggac gattatgaaa
      181 aagaatccgg caccgttgcg gatactgtga tgaggatgac agagccgctc gctgaagata
      241 aaccaatatc tttttcggca ggtctaaaag gaaaaccgct ggcttcacgc atctctcccc
      301 tgatacacac cagcacgggc ggtgcttcca tggaggcatc atccctgtcc ggcctggaaa
      361 gcaggctaaa cacctccaaa aacaatggca gtccgctacc ccaagaagca cgttctttta
      421 tggaaaaccg ctttggtacc gatttcggcc atgtaaaaat acacacggat accggtgccg
      481 ttcaaatgaa caaagaactg aatgctcagg cattcactca cggcaaccat atttacttca
      541 acagcggaat gtttgataca gcaagttttt caggaaaaca tttgctggcg cacgagctga
      601 cacatgtggt gcagcaaaat ggcaaccatt tttccgtaca acgggagacc acccctgaag
      661 aaaaagagtt acgcgaaaaa gccgaactgc tggaaacaga gattctggca gatcccgcct
      721 atgccggatt aaaaagccaa tcgaaaagcc gtgtgaaaac catcatcaaa aaagcccaaa
      781 aaaagccttt aggagaaaaa attggccaaa gaaattatta tttgataaaa ctgaaaaccg
      841 ccatcactac accatttaaa ggccaggata ccggcaatga agaatacgga tgttctctgg
      901 aagcagataa agagaatcgt gaagaagtgg aaaaatttct gaaaatagaa ggtaaatatt
      961 ggagtaacat aggttttgaa aacgtcgaag aagaaatagt ggcgggagga aaaaggaaag
     1021 taatgaggga aggtcaggat ggcaagatat tttatgttga cagaagtgat gcaaaaaata
     1081 ttcgtgtgct gataaaagtg aaactaaacg gaaatccaga cgaaattgta aaaatcaaag
     1141 cccttgaaga cgccattgaa agagaagcct ccaccacagg gtacacactc gacatagagt
     1201 ttgtggataa atccggtttt gatgtctttg aattttcagt aagtttttgc gaatgggcca
     1261 attccggcaa ttgggcttcg ggccctgttt ctttgtcgca tgaggttcac catgcacttg
     1321 gtttggacga tcgttatgat tatatcgaaa gtcatgctca caatcctatg atgaatgttc
     1381 ccatgagact ggtgtggttt gaagtacaaa tggataaaac gatgacaacc agggatgact
     1441 atagtaagat gagttccagc gaaaatccga tgctgtcgga agacgtatgc tctgtagcat
     1501 ttgggtcaaa ggccaacagg gataaatgta ttgaggcccg aaaagaactg gaccctcctg
     1561 gaatacccgg acaatgataa tgcccggttg ggatattcgg ctgcagtact gtcagacaag
     1621 tgggcgcatg gctgcgcgcc ggacatgcgc tcatggcaag tcgcgctccc actaaaagca
     1681 tagtcagtcg gccacttcaa ccattaccag ttgtggcgaa gtttgcaact tcgcactaaa
     1741 tagtcgctcc ttaaaatagt tataaacatt tgaatgtcaa taatttgtag taattttttt
     1801 gtgtttatat ttgcaggaat ttataccttt
//
LOCUS       DAOSFP010000425         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_64979_length_1829_cov_2.188839, whole genome shotgun sequence.
ACCESSION   DAOSFP010000425 DAOSFP010000000
VERSION     DAOSFP010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64979_length_1829_cov_2.188839"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="PLI16_10230"
     CDS             complement(<1..1124)
                     /locus_tag="PLI16_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH84976.1"
                     /translation="MEEITGRLNRKNYLLSIMVIGILYFIFGFVTWLNGALIPFLRTA
                     CELSDVMAYLVTFAFYISYFVMAIPSSKIIERFGFRNGMAIGLAVMSAGALIFIPAAL
                     TRSYWLFLTGLFVLGTGLALLQTAVNPYVTILGPIESAAKRISIMGICNKVAGVLAPI
                     LLASFLLKDAEQIKITLSETTDALQRAALLDELAGRMIMPYLFMTVFLAIMIILVRFA
                     KLPEVNQVNGDDTQHLAQDQSIFQHRNLILGVVALFFYVGVEVISGDTIIRYGESLNI
                     AMSSAKYFTSLMLVFMVIGYFVGVALIPKVLSQRNALTGSAVTGIIFTFAAVLTPAQM
                     ILNIPFIDLVTFKTVTLTIPYTVLFVAMLGLANSLVWPAI"
     gene            complement(1297..>1829)
                     /locus_tag="PLI16_10235"
     CDS             complement(1297..>1829)
                     /locus_tag="PLI16_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOH84977.1"
                     /translation="AKEIRKTNKEIPIIFLTAKNLKEDRIKGFQIGCDDYITKPFSTE
                     ELSLRIEAIIRRAVKKSQFPLIDNVEVYTLSTFTFDYTNMILKSSAGEQYLTRKEADL
                     LKLLCVNKNRLVHREIALKSIWGDSDYFTGRSMDVFITRLRKYLKDDDKIRIANIHGT
                     GFKLEVNEETPEEPIQ"
BASE COUNT          561 a          397 c          375 g          496 t
ORIGIN      
        1 catatagccg gccacaccag cgagttggcc agtccgagca tggctacaaa cagcacggta
       61 taaggaatag tgagagtaac tgttttaaaa gtaaccagat cgataaaggg aatattcaaa
      121 atcatttgtg ccggtgtaag aaccgccgca aatgtaaaga taatacctgt aacagcagaa
      181 cccgtcaggg cattgcgctg cgaaagaact ttgggaatca aagcgacacc gacaaaatac
      241 ccgatgacca taaaaacaag catcagcgac gtaaaatatt ttgccgagct catggcgatg
      301 ttcagcgatt cgccataacg gatgatagta tcgccggata ttacctccac acctacataa
      361 aaaaacaatg ccaccacgcc cagtatcaaa ttgcggtgtt gaaaaatgct ttgatcctgt
      421 gctaaatgct gtgtatcatc gccgttcacc tggttgactt ccggcaactt tgcaaaacgc
      481 accagaatta tcattatcgc taaaaaaact gtcatgaaca ggtacggcat aatcatgcgt
      541 ccggccagtt catccagcaa tgcagcacgt tgcaaggcat cggtagtctc tgaaagggtt
      601 atttttatct gctcggcatc tttcagtaaa aaagaagcaa gcaatatagg agcaagcact
      661 cccgccacct tattgcagat acccataata ctgatgcgtt tggcagcact ttctatcggt
      721 ccgagtatgg tcacataagg atttaccgct gtttgcagca aggcaagtcc tgttccaagc
      781 acaaaaaggc cggtgagaaa caaccaataa gaacgggtta atgccgcggg gataaaaatc
      841 agcgcccccg ccgacatcac ggcaagtcct atggccatac catttcggaa cccgaatcgt
      901 tcaataattt tcgacgatgg tatggccatc acaaaataag aaatataaaa ggcaaaggtt
      961 accaaatagg ccatcacatc gctaagttca caagcggtcc gtaaaaaagg tatcagagcg
     1021 ccgttcagcc aggtaacaaa accaaagatg aaatataaaa tgcctattac catgatagat
     1081 aacaggtaat tttttctgtt cagacgccct gttatttctt ccatgttatg ttttttttaa
     1141 aatcaatgag atagtatttt tataagcatt tatttctgcc gtacatcttg taccaaatgc
     1201 aaaaatacaa tattgataat acaatagaaa taaaacctga tgatattgtc aacagcagga
     1261 tgtaataatt aaaacggcac tatgccttaa actactctat tgtatcggtt cttcaggtgt
     1321 ctcttcattc acttcgagtt taaatcccgt accgtgaatg ttggcaatac ggatcttatc
     1381 atcgtctttc aggtacttcc ttaaacgggt gatgaaaaca tccatgctgc gtccggtaaa
     1441 ataatcgctg tcgccccaga ttgatttcag ggcaatttcc ctgtgtacca gcctgttttt
     1501 gtttacacac agaagtttca gcaaatcggc ttcttttcgt gtaaggtatt gctcgccggc
     1561 agatgatttc agaatcatgt tggtgtaatc gaaagtaaaa gtactcaggg tatatacttc
     1621 cacattgtcg ataagcggaa actgcgattt ttttacagca cgcctgatga tggcttcgat
     1681 acggaggctg agttcttcgg tactgaaagg cttggtaatg taatcgtcgc atccaatctg
     1741 gaaccctttg atacggtctt ctttaaggtt tttggctgtc agaaaaataa tgggaatttc
     1801 cttgttggtt tttcttattt ctttggcaa
//
LOCUS       DAOSFP010000426         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_65068_length_1827_cov_3.579571, whole genome shotgun sequence.
ACCESSION   DAOSFP010000426 DAOSFP010000000
VERSION     DAOSFP010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65068_length_1827_cov_3.579571"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="PLI16_10240"
     CDS             <1..1191
                     /locus_tag="PLI16_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOH84978.1"
                     /translation="EHDKDTMLEADHLIDIGPGGGINGGYIMAQGTPAEVMKTNSLTA
                     LYLNEVHQIKIPSTRRKANGNVLKLIGACGNNLKNITATFPLGTFICVTGVSGSGKST
                     LINQTLYPILSQHFYRSEQKPLEYKKIEGIKNIDKVIEIDQTPIGRTPRSNPATYTGV
                     FDDIRKLFALVPESKIRGYLPGRFSFNVKSGRCETCAGAGVRTIEMNFLPDVYVSCES
                     CNGKRYNRETLEIKYKGKSICDVLEMNIDEAVDFFSQIPAIIQKIKTLKDVGLGYIRL
                     GQQSTTLSGGEAQRVKLATELAKKDTGRTVYILDEPTTGLHFEDINVLLKVLDKLVEK
                     GNTVIVIEHNMDVIKMADYIIDLGPEGGNRGGQIICEGTPESIINCSNSYTAEFLKPV
                     LLKQ"
     gene            1228..>1827
                     /locus_tag="PLI16_10245"
     CDS             1228..>1827
                     /locus_tag="PLI16_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOH84979.1"
                     /translation="MHKLDNEEEEQLIDALEPKTWQEIKIQDSWQIFKVMSEFVDGFE
                     KLTKIGPCVTIFGSARTRPDNNYYEMAQDIAFKLVRLGYGVITGGGPGIMEAGNKGAY
                     FGKGKSVGLNIELPFEQKSNDFIDRDKNLSFDYFFVRKTMFVKYAQGFIVMPGGYGTL
                     DEFFEAITLIQTKKNVNFPVVLVGKDYWKGLISWIKDKVL"
BASE COUNT          580 a          370 c          424 g          453 t
ORIGIN      
        1 gaacacgaca aagataccat gttggaggcc gatcacctta tcgatatagg gccgggcggt
       61 ggtataaacg gcgggtacat catggctcag ggcacaccgg ccgaagtgat gaagaccaac
      121 agccttaccg cactgtacct taacgaagtg catcagataa aaataccttc aaccaggcgt
      181 aaggcaaatg gaaacgtgct gaagctgatt ggtgcctgcg gcaataacct gaaaaatatc
      241 acagccacat ttcctttagg aacatttatc tgtgttaccg gagtgtcggg cagtggaaaa
      301 tccaccttaa tcaatcagac cttgtatccg attttaagcc agcattttta tcgttcggaa
      361 caaaaaccac tggaatacaa aaaaattgaa ggaataaaga atatcgataa agtcattgaa
      421 attgaccaaa ctcccattgg ccgcaccccg cgttccaacc cggctaccta tacaggcgtt
      481 ttcgatgata taagaaaatt gtttgcactg gtgcccgaat caaaaataag agggtatctt
      541 cccggccggt tttcgtttaa cgtgaaaagc ggtcgttgcg aaacatgcgc aggcgccggt
      601 gtgagaacca tcgaaatgaa ttttttgccc gacgtgtacg tctcctgcga aagctgcaac
      661 gggaaacggt ataaccgcga aacactagag ataaaatata aaggaaagtc aatatgcgac
      721 gtgctggaga tgaatattga tgaggcagtg gatttttttt ctcagatacc cgctatcatt
      781 caaaaaatta aaacacttaa ggatgtcggt ctgggataca tccggctggg acaacaatcc
      841 accacactgt cgggcggcga agcacaaagg gtgaaactgg ccactgagct cgcaaaaaaa
      901 gataccggac gtactgtata tattcttgat gagccaacca ccggcttaca ttttgaagat
      961 atcaacgtgt tgctgaaggt gctcgataaa ctcgtcgaaa aaggtaatac tgtcatagtc
     1021 attgagcaca atatggatgt aataaaaatg gcggattaca ttattgacct cggtcccgaa
     1081 ggcggaaacc gcggcggaca gattatttgc gaagggacac cggaatccat catcaattgc
     1141 agcaatagtt ataccgctga atttttaaaa ccggtattgt tgaaacaata attttttatt
     1201 ctgacagtta ttataaaaaa atatattatg cataaactcg ataatgaaga agaagaacaa
     1261 ctgattgatg cactggaacc taaaacctgg caagaaatta agatacagga ctcctggcag
     1321 atttttaaag taatgtccga gtttgtggat gggtttgaaa aacttaccaa gataggaccc
     1381 tgcgttacta ttttcggatc ggctcgcaca cgccccgata ataattatta tgaaatggct
     1441 caggatatcg cctttaaact ggtgcgcctg ggttatggcg ttatcacggg tggcggcccc
     1501 ggtatcatgg aggccggtaa taagggagcc tactttggaa aaggaaaatc cgtgggactg
     1561 aacatcgagt tgcctttcga acaaaaatca aacgatttta tcgacaggga taaaaacctt
     1621 tccttcgatt atttttttgt gcgcaaaacc atgtttgtca aatacgcaca aggattcatc
     1681 gtaatgccgg gaggttatgg caccctcgac gagttctttg aagctatcac actgatacag
     1741 acaaagaaaa atgttaattt tcctgtggtg ttggttggaa aagattattg gaaaggcctg
     1801 ataagctgga ttaaagacaa ggtgctg
//
LOCUS       DAOSFP010000427         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_67184_length_1788_cov_3.274091, whole genome shotgun sequence.
ACCESSION   DAOSFP010000427 DAOSFP010000000
VERSION     DAOSFP010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67184_length_1788_cov_3.274091"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..881
                     /locus_tag="PLI16_10250"
     CDS             204..881
                     /locus_tag="PLI16_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOH84980.1"
                     /translation="MLHALVSGFILGLTLAVVFGPAFFSLLQTSILRGFRIGAYLAIG
                     IMLSDFTLIALSFLGVSQLITNDDYSKLFGIIGGLILLGYGAFVFRKKVVYNESEINK
                     LEKTSNSIFGDSPSKPYVYILKGYFLNLLNPFLLITWMGIMGYVAAEYNSDIKKLAVF
                     FGVALGTVFSTDLLKCFVANKLKKLLRPNILSIINRGLGTLLFLFGLYLIIKTILGFL
                     HTGIMIP"
     gene            complement(917..1459)
                     /locus_tag="PLI16_10255"
     CDS             complement(917..1459)
                     /locus_tag="PLI16_10255"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOH84981.1"
                     /translation="MGKLAELTDNGIKIHLFTGNHDMWMFRYFEQELGIDISRKPVVK
                     EINGLKILIGHGDGLGPGDYGYKMIKKIFASKTCQWLYARLHPNFAMGLGLFFSRRSR
                     LARGDIDRIYKGDNGERLIVYAKEMLKKEHFDLFVFGHRHLPIDLKIGENSRYINLGD
                     WLQHFSYALLDGKDFITKKY"
BASE COUNT          480 a          388 c          372 g          548 t
ORIGIN      
        1 tttatgtaaa atcatctact atcggcaagg gcactctgtt caggatagag ttaagtaaaa
       61 gcaggaccgc actgaagaaa gaataagttg gccgggagta tctcattatg aaaaaagtag
      121 tttgcatgct gaatccggaa atttttcatg caataaatgg gatttaattt ttaaaaaata
      181 ataatttagc aaaaatttta tcgatgttac atgccctggt cagcggattt attttgggat
      241 taacccttgc tgtagttttt ggcccggcat ttttttcgtt gttgcaaacg agcatcctca
      301 gggggttccg gataggtgca tacctggcca taggcattat gctgagcgat tttaccctta
      361 ttgccttaag ttttttaggt gtatcgcaat tgattaccaa cgacgactac agcaagttgt
      421 ttggcatcat tggaggcctt attttattag gttacggagc atttgtcttt cgcaaaaagg
      481 tggtttacaa cgaaagtgaa atcaacaaat tggagaagac aagcaacagc atcttcggtg
      541 actcgccctc caagccatat gtttatatcc tgaaaggata ttttctgaat cttttaaatc
      601 cctttctttt gatcacctgg atgggcatca tgggctatgt ggccgccgaa tataattctg
      661 atataaaaaa actggctgtt tttttcggtg ttgccctggg caccgtgttt tccaccgacc
      721 tgttaaaatg ttttgtagcc aataaactca aaaagttgtt gcgtcccaat atcctttcaa
      781 ttatcaaccg cggattggga acattattat ttctttttgg cctctacctg ataataaaaa
      841 cgatactggg ctttttacac acgggaatta tgattccctg atcatgcatt gtgcagcata
      901 catttgaagg tgataatcaa tactttttgg taataaaatc tttaccgtcg agcaaggcgt
      961 acgaaaaatg ttgcagccag tcgccaaggt tgatataccg tgagttttcg cctattttca
     1021 ggtcaatagg aaggtgccgg tgcccgaaaa caaacaggtc gaagtgttct tttttcagca
     1081 tctctttggc atacactatc aggcgttcgc cattgtcccc cttataaatc cggtcgatat
     1141 cgcctctggc caggcggcta cggcgcgaaa aaaacaggcc caaccccatg gcaaaattcg
     1201 gatgcagccg ggcataaagc cattggcagg ttttgctggc aaaaatcttt tttatcattt
     1261 tatagccata atcgccgggg cccagcccat cgccatgacc tatcaggatc tttagtccgt
     1321 tgatttcctt aaccaccggt ttcctgctta tatcaatacc cagttcctgc tcaaagtatc
     1381 tgaacatcca catatcgtgg ttgccggtaa acaggtgtat ttttatcccg ttgtcggtca
     1441 gttcggcaag ttttcccaaa agccgggtat atcctttggg tattaccgtc ttatactcaa
     1501 accagaaatc aaagaggtcg cccaggattt aaatctcggc agcatcggcg tttatcatat
     1561 ccagccatgc cacaaatttt ttttcacgcg ccaggctgct gtggtaatcg ggtatcccaa
     1621 agtggcagtc cgacacaaaa tatattttac ttgctgtacc tgtcaaaact tatagatttt
     1681 attcaaccat gcaggcaatt cttcttcggt gatgcctctt ttcaaaatac gtccgtcggc
     1741 atcgacaagc acagccagcg ggatgtcatc gatgcaatag gtttttac
//
LOCUS       DAOSFP010000428         1768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_68350_length_1768_cov_3.916521, whole genome shotgun sequence.
ACCESSION   DAOSFP010000428 DAOSFP010000000
VERSION     DAOSFP010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68350_length_1768_cov_3.916521"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1340
                     /locus_tag="PLI16_10260"
     CDS             42..1340
                     /locus_tag="PLI16_10260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HOH84982.1"
                     /translation="MLSVIVIVTSILLLLLLLQRIVKSEWLDPMFVSALYWGGLIVFS
                     VFFSKVFHFELYLPGLIPLFLLLVSFIIGSLVAKKLIFASGQKSAHSMVPPKIDCLQH
                     KRIKWLTIIFSSLGMAAIVLQLRFLDIHIGSFADLLYAANTISVIRYTGGTDMPKYGL
                     ILMAFLYSAGFLGGIYALSGKGFWNKMISLLPFAVMLVFTITNSVKSGFIFMLILWVS
                     GYIAVYVFMKNGQLKRIGRLVSSVVLIFVFILALIPLTQILRSGKVSADTEYVNAGGL
                     SYFCSVNTFTIWYKSYSHENITGFKYTLSGINNFFFGEREIGLYGEKNVEVGKYNGNP
                     VQTNVYTMARGLIEDFTLPGALLVLFLAGIIAQILYERTKKNSVICMGLLSLFYSVLL
                     WSITVNILNYNTIILCWLISFATLFFIKKSNFAAPQKTAD"
     gene            1343..>1768
                     /locus_tag="PLI16_10265"
     CDS             1343..>1768
                     /locus_tag="PLI16_10265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOH84983.1"
                     /translation="MKRGLFLTVNYFQAAMTIELIKSLLAISGVEHFRIVVVDNSASS
                     EEYEQLLNFKKLTEGDVEILKTPSNLGYFGAVNFALKHPGNSKGYDYVIVSNNDIEIR
                     DRQFFQNLLELQSDAQVIAPDIISLTTGRHQNPHRTSPVS"
BASE COUNT          479 a          331 c          374 g          584 t
ORIGIN      
        1 tatgttgctg ttgataaaaa atttgaataa aaaactcaga aatgcttagc gtcattgtca
       61 tagtaacatc aattcttttg ctgctgctgt tgctccaacg gatagtgaaa tcagaatggc
      121 tggaccccat gtttgtttca gcgctgtatt ggggcggcct gatagttttc agtgtgtttt
      181 tttcgaaggt ttttcatttt gaactttact tgcccggatt gatacccttg tttttattgc
      241 tggtgtcttt tattatcggt tcattggtgg caaaaaaact gatttttgca agcgggcaaa
      301 aatccgccca tagcatggtg cccccaaaaa tcgattgcct gcaacataaa cgcatcaaat
      361 ggcttacaat cattttttca tcgttgggaa tggctgccat tgttttacag cttagatttc
      421 ttgatatcca tattggcagt tttgctgatt tgctgtatgc cgccaatacc atttcggtaa
      481 tcaggtatac tggtggcacc gacatgccca aatatggact tattctaatg gcctttctgt
      541 attctgccgg gtttctgggt ggcatatacg ctttgtcagg caaaggtttt tggaataaaa
      601 tgataagttt gcttcccttt gcagtgatgc tcgtgtttac cattaccaac agtgtaaaat
      661 cgggttttat ttttatgttg attctttggg tttctggata tatagccgta tatgtattca
      721 tgaaaaacgg acaactaaaa aggattggca gattggttag cagcgtagtg ctgatttttg
      781 tttttattct tgccctgata cccctcacac aaatactgag gtcgggaaaa gtcagcgctg
      841 atactgagta cgtgaatgcc ggtggcttat cgtatttttg ttcggtaaac acatttacca
      901 tttggtacaa atcctactcg cacgaaaaca taacggggtt caaatatacg ctgtcgggga
      961 taaataattt tttcttcggt gagcgtgaaa ttggcttata tggagaaaaa aatgtggaag
     1021 ttggtaaata taacggtaat cctgtacaga ccaacgttta tactatggca cgcggactta
     1081 tcgaagattt taccttgccg ggcgctttgc tcgtactttt tttagccggc attattgcac
     1141 agatattata tgaaagaacc aaaaaaaact cagtgatctg catggggctg ctttcgctgt
     1201 tttacagtgt tttgttgtgg agcataaccg tcaacatatt aaattacaat accattatac
     1261 tttgctggct gatttctttt gcaacactgt ttttcattaa aaaaagtaat tttgcagcgc
     1321 ctcaaaaaac cgcagattga ttatgaaaag gggacttttt ctgacagtga actattttca
     1381 ggcagccatg actatcgagc tgataaaaag tcttcttgcc atttcgggtg ttgaacattt
     1441 caggattgtc gttgttgaca attcggcatc ctcagaggaa tatgagcaac tgctcaattt
     1501 taaaaagctc actgaaggtg atgttgaaat cttgaaaacc ccgtcaaatc tgggatattt
     1561 cggtgcggta aattttgctt taaaacatcc tggcaatagt aagggatatg attatgtaat
     1621 tgtgtctaat aacgacattg aaattcgcga ccggcaattt tttcaaaacc ttcttgaact
     1681 gcagagcgat gctcaggtta ttgcccccga tattatttcg ttaaccaccg gccggcatca
     1741 aaatccacac aggacaagcc ctgtgagc
//
LOCUS       DAOSFP010000429         1748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_69527_length_1748_cov_3.179563, whole genome shotgun sequence.
ACCESSION   DAOSFP010000429 DAOSFP010000000
VERSION     DAOSFP010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1748
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69527_length_1748_cov_3.179563"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLI16_10270"
     CDS             <1..628
                     /locus_tag="PLI16_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOH84984.1"
                     /translation="NTFFKWLKTEISVYFSKGIDFMSLLSTADSVNLGYTLAPIYQVS
                     NISSVNIYGVEADVSAPIGKYLRAYVNYTFNHSTVSRFVPNTEADRDLTGKFLPDVPM
                     HQFSTGISLRTDYINFSIAGKYTGTRWVKDDNTVDNIFMMTDRYPARFITDIKVWQNV
                     GPVDFSIDIDNLFNIVYINSKGYKSPGRMVFGKISYTFNNKKSKKTKY"
     gene            643..1710
                     /locus_tag="PLI16_10275"
     CDS             643..1710
                     /locus_tag="PLI16_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84985.1"
                     /translation="MKKINLLVLLLAVAVLTSCNQTKKENTAEGVIIPADTADKTNDF
                     YDNADTYPLKGDRKIEVGGEVANPGIVDFSKMTKRSVIVKETIYKAPADSFYGAFRYD
                     GYSVYDILNQIVLKKKNEEAFPPIIDLFVEIENDKGEKVVLSWGEIYYPIHRHEIIIA
                     TDVMRIVPSKTKELWTLPENTRLVVGSDLITARNISNPVKISVVSYPLDVPVNKEMKP
                     LYSPEFKITDGTKDLKTIKELPKDLLVQNYPAIFYGRGKGIHSTQPFKGVMLKDMLAE
                     DFPLSNDLLKRGLLLAVAKDGYRGVFTYSEVMNRNDQAELLLIASPEEKTDAVFRLYP
                     AMDFFSDRAIKALTEIRCVLR"
BASE COUNT          527 a          379 c          384 g          458 t
ORIGIN      
        1 caacacgttt ttcaaatggc tgaaaactga aatttccgtg tatttttcta aaggtattga
       61 ctttatgtcg ttgctttcca ccgccgactc tgttaatctt ggctacacac tggcgcccat
      121 ttatcaggtt agcaacatca gcagtgtaaa catctatggc gtggaagccg atgtttctgc
      181 ccccatcgga aagtatctga gagcctatgt aaactatacg ttcaaccact ccaccgtgag
      241 ccgttttgta cccaacacgg aggccgaccg cgacctcacc ggaaagttcc tgcccgacgt
      301 gcctatgcac cagttttcaa caggcataag cctccgaacc gattatatta atttcagcat
      361 cgccggaaaa tacaccggta cccgctgggt taaagatgat aataccgtgg acaacatttt
      421 tatgatgaca gaccggtatc ctgcacgctt catcaccgat atcaaggtgt ggcaaaatgt
      481 cggccctgtt gatttcagca tcgatatcga taatttgttc aatatcgttt acatcaacag
      541 caagggttac aaatcgcccg ggcgcatggt gttcgggaaa ataagctata cctttaacaa
      601 taaaaaatct aaaaaaacga aatattaatt aaatcaaata atatgaaaaa gatcaactta
      661 ttagttctgt tgttagcggt tgctgtgctg acctcatgta atcagacaaa aaaagagaat
      721 accgcagaag gtgttattat acctgccgat acagcagaca agaccaatga cttctacgat
      781 aacgccgaca cgtatccatt aaaaggcgat cgtaaaatcg aggtgggagg ggaagtggct
      841 aacccaggca tcgtggattt ttcaaagatg accaaaagaa gtgttatcgt gaaggaaact
      901 atttataaag cgcctgctga ttctttttat ggcgcgtttc gttacgacgg atattctgtt
      961 tacgacatac tgaatcagat agtcctgaaa aagaagaatg aggaagcttt tccgcctatt
     1021 atcgacctgt ttgtggaaat tgaaaacgac aaaggcgaaa aagtggtgct ttcgtggggc
     1081 gagatatact atccgattca ccgtcacgaa atcattatcg ctaccgatgt tatgcggata
     1141 gtgcccagta aaaccaaaga actgtggact ttgcccgaaa acacccggct ggtggtgggc
     1201 agcgacctga taacggcacg gaatatctca aacccggtaa aaatctcggt agtgtcatac
     1261 ccgcttgatg ttccggttaa caaggagatg aaaccgctgt acagcccgga gtttaaaatt
     1321 accgacggca ccaaagacct taaaaccatt aaagaattac ctaaagacct tttggtacaa
     1381 aattaccctg ctatctttta cggccgagga aaaggcatcc acagcaccca gccttttaag
     1441 ggtgtgatgc tgaaagacat gcttgcagag gattttccgt taagtaacga cttgcttaaa
     1501 cgaggccttt tattggctgt cgccaaagat gggtaccgtg gtgtttttac ttacagcgaa
     1561 gttatgaacc gtaatgacca ggctgaatta ttgctgattg caagccctga ggaaaaaacg
     1621 gatgctgttt tcaggctgta tccggccatg gattttttca gtgaccgtgc catcaaagcg
     1681 ctgacagaaa tacgttgtgt gctcaggtaa aaaactctaa tgaggtttta tacaacgaat
     1741 tgtttata
//
LOCUS       DAOSFP010000430         1710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_71808_length_1710_cov_4.508761, whole genome shotgun sequence.
ACCESSION   DAOSFP010000430 DAOSFP010000000
VERSION     DAOSFP010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71808_length_1710_cov_4.508761"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLI16_10280"
     CDS             <1..1067
                     /locus_tag="PLI16_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOH84986.1"
                     /translation="RKSDDIKCYGAYVLNPAENSIITILSKITLLATGGAGNVYSNTT
                     NPTVATGDGVAMVFRAKGEIKDMEFFQFHPTSLYNPKERPSFLISEAVRGFGGILKTI
                     KGNEFMQKYDPRLSLAPRDIVARAIDTEMKISGDEHVCLDCRHLDSNKLIEHFPTIYA
                     KCLSIGIDITKEMIPVVPAAHFLCGGIQTDEYGRTSIKNLYATGECACTGLHGANRLA
                     SNSLLESVVFSHRAALDALEKFSTIGLNTGIPDWNAEGTVLNEEMILITQTTRELREI
                     MSNYVGIVRSNLRLKRAMDRLDILYHETEKLYDQSVLTVEICELRNMINVAFLIISMA
                     QERKESRGLHYSLDYPDYKK"
     gene            1072..1590
                     /locus_tag="PLI16_10285"
     CDS             1072..1590
                     /locus_tag="PLI16_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOH84987.1"
                     /translation="MALIKKVKGIVPSWGANCFFADNATLAGDVIMGDDCSVWFNAVV
                     RGDVHSIRIGNNVNIQDCAVIHCTYQKASVSIGNNVSIAHGAIVHGCTIQDNVLIGMG
                     AIIMDGVVIESNTIIAAGAVVLENTLVESGSIYAGMPAKKIKNIDKSLLEGQVNRISK
                     NYMMYAGWYDAD"
BASE COUNT          510 a          400 c          375 g          425 t
ORIGIN      
        1 acaggaaatc cgatgatatc aaatgttatg gcgcttatgt gttaaatcct gctgaaaata
       61 gtatcattac catcttatca aaaatcactt tgctggctac cggcggtgcg ggcaatgttt
      121 acagcaacac caccaacccc acggtagcta ccggcgacgg cgttgctatg gtttttcgcg
      181 cgaaaggaga aataaaagat atggagtttt ttcagtttca tcccacttca ttatataatc
      241 ccaaagaacg gccatccttt ctgatttcag aggctgttcg gggatttggc gggatactga
      301 aaacaataaa aggaaatgaa ttcatgcaaa aatatgatcc ccgcctgagt ctcgcacccc
      361 gcgatattgt cgcacgtgcc atcgacacag agatgaaaat cagcggcgac gaacatgtat
      421 gtcttgactg ccgccacctc gacagcaaca agctcatcga acattttcct accatctatg
      481 caaaatgcct tagcataggt attgacatca ccaaagaaat gatacccgtg gtacccgctg
      541 cacatttttt gtgcggggga atacaaacag acgagtacgg cagaacctcc ataaaaaatc
      601 tttatgctac cggcgaatgc gcctgtacag gcctgcacgg cgctaacagg cttgcctcca
      661 attcgctgct cgaatcagtg gttttttcgc accgtgccgc cttggacgca ctggaaaaat
      721 tcagcaccat tggcctcaat accggcatac ccgactggaa tgccgaaggt accgtgttga
      781 acgaagaaat gatcctgata acacaaacca cccgggaatt gcgtgaaatc atgagcaact
      841 atgtcggcat tgtccgttcg aacctccgct taaaaagagc catggaccgc ctcgacattt
      901 tatatcatga aacggaaaaa ctatatgatc aatcggtact gacagtagaa atttgtgaat
      961 tacgcaacat gataaatgta gcgtttctga taatctcaat ggcccaggag cgcaaagaaa
     1021 gcagaggact tcactattct cttgattatc ccgattataa aaaataattt tatggcacta
     1081 ataaaaaaag ttaaaggcat tgttccctcc tggggtgcca attgtttttt tgccgacaac
     1141 gcaaccctgg ccggcgatgt aataatgggc gacgattgca gcgtttggtt caatgccgta
     1201 gtccgcggcg atgtgcattc catacgtatc ggcaataatg tcaacataca ggattgtgcc
     1261 gttattcatt gcacttacca aaaagcttct gtttctattg gcaacaatgt ttccattgct
     1321 cacggcgcca ttgttcatgg ctgcaccata caagacaatg tgcttatcgg aatgggggcc
     1381 ataatcatgg acggtgttgt tatcgaaagc aacaccatca tcgccgccgg tgcggtggta
     1441 ctcgaaaaca cattggtaga atcgggaagt atctatgccg gtatgcctgc aaaaaaaatc
     1501 aaaaacattg acaaatcgct gctcgaaggc caggtaaacc gcatctcaaa aaactacatg
     1561 atgtatgccg gctggtatga tgccgattga ataaccaagt ttaaaagcga ccaagtatcc
     1621 catgcaagta aatggaaaaa actaccggac cgtatggatg gagcagggct cggtatttat
     1681 gattgaacaa aacctgcttc cttatgattt
//
LOCUS       DAOSFP010000431         1707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_72017_length_1707_cov_3.609564, whole genome shotgun sequence.
ACCESSION   DAOSFP010000431 DAOSFP010000000
VERSION     DAOSFP010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72017_length_1707_cov_3.609564"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLI16_10290"
     CDS             complement(<1..470)
                     /locus_tag="PLI16_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84988.1"
                     /translation="MKIPVTAGKNIILIFALLMVLVSCSKTRKEYYQDGVLKSELTYK
                     NKKLHGLQTWYYSNANKQMECTYQDGMIEGKLTRWSFNGKVEREEFYTRNLRNGKSTA
                     YFEDGHPFTEENYENDTLQGAYREYHPNGTLKINGNFEKGLYQGKWEYYDDKGL"
     gene            complement(486..>1707)
                     /gene="clpX"
                     /locus_tag="PLI16_10295"
     CDS             complement(486..>1707)
                     /gene="clpX"
                     /locus_tag="PLI16_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004308630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOH84989.1"
                     /translation="CSFCGKKRSEVNLLIAGLEGHICDGCVEQAELILKEEYMVRKKT
                     GVSHKFELPKPAEIKHHLDQYVIGQDQAKRVLSVAVYNHYKRISQFSHKKSADDVEIE
                     KSNIILVGETGTGKTLLARTIAKMLHVPFCIADATVLTEAGYVGEDVESILTRLLQVA
                     DYNVEEAEKGIVFIDEIDKIARKSDNPSITRDVSGEGVQQAMLKLLEGAVVNVAPQGG
                     RKHPEQKMIQVNTENILFICGGAFNGIENKIGSRLMTSMIGYASGTKRKNIDKDNLMQ
                     YVAPQDLKSFGLIPELVGRLPIISHLNPLTRDTLKRILTEPKNALTKQYIKLFEMDKI
                     KLTFDEAALDLIVDKAVEYKLGARGLRSIFEIIMTDTMFEMPSNNDTSDLHITRAFAE
                     EKINRIDRAMLMVA"
BASE COUNT          404 a          408 c          353 g          542 t
ORIGIN      
        1 ttcaatcctt tatcatcgta atattcccat ttcccctgat acaggccttt ttcaaaattc
       61 ccgtttattt tcagcgttcc gttgggatgg tattcgcgat aggcaccttg cagggtatcg
      121 ttctcataat tctcctcggt aaaagggtga ccgtcttcaa aatacgcggt gcttttgcca
      181 ttgcgcaggt tacgggtata aaactcctca cgttcaactt taccattaaa actccaccgt
      241 gttagttttc cttcaatcat cccgtcctga taagtacatt ccatttgctt gttagcatta
      301 gaatagtacc aggtctgcag cccatgcagt tttttgtttt tgtaggtaag ctccgatttc
      361 aagacaccat cctgataata ttctttgcgt gttttgctgc acgataccaa taccatcaac
      421 aaagcaaaaa ttagaataat attttttcct gctgttactg gaattttcat aacgcgacaa
      481 tggtattatg cgaccatcag catagcgcgg tcaattctgt ttattttttc ctcggcaaag
      541 gcacgggtaa tatgcagatc gctggtgtcg ttatttgaag gcatttcgaa catggtgtcg
      601 gtcatgataa tttcaaaaat tgaacgcagg cctctggcgc caagcttgta ttccacggcc
      661 ttatcgacga taaggtccag tgctgcctca tcaaaggtaa gtttgatttt atccatttca
      721 aacaatttaa tatattgttt ggtcagggca tttttgggtt cggtgagtat gcgtttcagg
      781 gtatccctgg tcagcggatt caggtgactg atgatgggca ggcgtcctac cagttcgggg
      841 ataagcccga aacttttcaa atcttgcgga gccacatact gcatcaggtt atccttgtcg
      901 atattttttc gttttgtccc gctggcatag ccgatcatgc tggtcatgag ccgcgacccg
      961 attttgtttt caatcccgtt aaaagctccg ccacagataa acagaatatt ttcggtattg
     1021 acctgtatca ttttttgttc cggatgtttt cgtccaccct gcggcgccac attgacaacc
     1081 gcgccttcga gcagtttcag catagcttgt tgcacacctt cgccagacac atcccgtgtg
     1141 atggaaggat tgtcgctttt acgtgctatc ttatctatct cgtcaataaa aactatgcct
     1201 ttttcggctt cttccacatt atagtcggcc acctgcaaca ggcgtgtaag aatgctttca
     1261 acgtcctcgc ccacatagcc ggcttcagta agtaccgtag catcggcaat acaaaatggc
     1321 acatgcaaca ttttggctat ggtgcgagcc agcaaggttt tcccggtgcc ggtttcgccc
     1381 actaaaataa tgttggattt ttcaatctcc acgtcatcgg cagatttttt gtggctgaac
     1441 tggctgatcc ttttgtaatg attataaacg gcaaccgaca gtactctttt ggcctgatcc
     1501 tgccctatca catactggtc gagatggtgt tttatctcag caggcttagg caattcgaat
     1561 ttatgggaaa cgccggtttt tttgcgaacc atatattcct ctttaagtat gagttccgcc
     1621 tgttctacgc agccatcaca aatatggcct tccagtccgg caatcagcag attgacttcg
     1681 gaccgctttt taccgcaaaa agaacat
//
LOCUS       DAOSFP010000432         1697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_72647_length_1697_cov_3.978076, whole genome shotgun sequence.
ACCESSION   DAOSFP010000432 DAOSFP010000000
VERSION     DAOSFP010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72647_length_1697_cov_3.978076"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLI16_10300"
     CDS             <1..522
                     /locus_tag="PLI16_10300"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOH84990.1"
                     /translation="DKMAAESKSVFAADPRGRGWRKVVASPVPLKIGNKNTIENIARN
                     GQIVIAVGGGGIPVYYKRPNFLEGIDAVIDKDLASSLLALQIHADEFYILTDVPKVCI
                     NFHKPDEKHLDVMSVEEARRYLAEGHFAEGSMAPKIRAAINFTEKGKGEAIITEASQL
                     SNKNCGTKIVKSK"
     gene            530..1336
                     /locus_tag="PLI16_10305"
     CDS             530..1336
                     /locus_tag="PLI16_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HOH84991.1"
                     /translation="MCEIICPQCKATLAEHAKFCHVCGYKLLDDNAQLNDTAQPNDTV
                     EDKTIDMKDFTDVHPEEVMTAIKSGNIFKRAINIMFKPKTEWEGVTAEKPRVPMLIFG
                     YSLVFAIIPVVSLILGYGLIGHKITVAWDNSFGIGFMQAFVFILASFTAIIVGAVVIN
                     AMAPAFKTEKNFGRAMQLTTYSFTPVFFAGALFFFPFLSVLANLAGLYGVFVLLIGLP
                     IVMRTPKNDQIGYFFASIGVLFGIYYTVKWTLALTFALASSAMWHMHYSF"
     gene            1356..>1697
                     /locus_tag="PLI16_10310"
     CDS             1356..>1697
                     /locus_tag="PLI16_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH84992.1"
                     /translation="MELPEINELGEKVKHWQTEETRYYRLLPLKNYVFSVMAVLGITA
                     TCLPWADITVGFYNKAMAVGLHFFSGWIIFLIYIAVIGIMLFNKHLKVDIKLAEKAPA
                     YAAFTTSALTLI"
BASE COUNT          466 a          351 c          380 g          500 t
ORIGIN      
        1 gacaaaatgg ctgccgaaag caaatcggtg tttgctgctg accctcgcgg acgcggctgg
       61 cgcaaggtag tggcttcgcc agtgcccttg aagatcggca ataaaaatac tatagaaaac
      121 atagcccgca atggacagat agtaattgct gttggcggtg gaggaatacc ggtgtattac
      181 aaacgcccca attttctcga aggtattgat gccgtgattg acaaagacct tgcctcttcg
      241 ttgctggcat tgcagataca tgccgacgag ttttatatcc ttaccgatgt gccaaaagtg
      301 tgcataaatt ttcataaacc cgacgaaaaa caccttgacg tcatgagcgt ggaagaagcc
      361 agacggtatc ttgccgaagg gcattttgcc gaaggcagca tggctccgaa gatacgggct
      421 gctattaact ttaccgaaaa agggaaaggt gaagcgatta ttactgaagc atcacaactt
      481 tcaaataaaa attgcggaac caaaattgta aaatccaaat aattatttta tgtgtgaaat
      541 catttgtcca caatgcaaag ccacgctggc tgagcatgca aaattttgtc atgtttgtgg
      601 ctacaagctg cttgatgaca acgcgcaact caatgataca gcacaaccca atgacaccgt
      661 cgaagacaaa accatcgata tgaaggattt taccgatgtg catcctgagg aagtgatgac
      721 ggcaatcaaa agcggtaata tcttcaaaag agccatcaat attatgttta aaccaaaaac
      781 agaatgggag ggggttactg ctgaaaagcc cagggtacct atgctgattt tcggatattc
      841 attggtcttt gccattattc cggttgtttc tcttattctt ggctatggac ttatcggtca
      901 caaaatcact gttgcctggg ataatagttt tggcattggt tttatgcagg cctttgtttt
      961 tatccttgct tcatttactg ccatcatcgt gggtgctgtg gttatcaacg ccatggctcc
     1021 ggcttttaaa actgaaaaaa actttggcag ggccatgcag cttacaactt attcctttac
     1081 accggtattt tttgcaggcg ccttgttttt ctttcctttt ctatcggttc ttgccaacct
     1141 tgccggcctc tacggtgtat ttgtgttact gattggcctg ccaatagtga tgcggacacc
     1201 caaaaacgac cagataggat attttttcgc ttctatcggc gtgctttttg gaatttatta
     1261 cacagtaaaa tggaccctgg cactcacttt tgctttggca tccagcgcca tgtggcatat
     1321 gcattattca ttttaaaact gtttgctgat acctgatgga attacccgaa atcaacgaat
     1381 tgggcgagaa ggtaaaacac tggcagactg aagaaacaag gtactacagg ctgctgccat
     1441 taaaaaatta tgtcttttcg gtaatggctg tattgggcat tacagctaca tgcctgccct
     1501 gggctgatat tactgtaggc ttttataata aggctatggc tgtggggctg catttttttt
     1561 cgggctggat aatatttttg atatacattg cagtcattgg gattatgttg tttaataagc
     1621 atcttaaagt tgatatcaaa ctggcggaaa aagcccctgc ttatgcggct tttacaacat
     1681 cagcacttac gctgatt
//
LOCUS       DAOSFP010000433         1657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_75396_length_1657_cov_5.723471, whole genome shotgun sequence.
ACCESSION   DAOSFP010000433 DAOSFP010000000
VERSION     DAOSFP010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75396_length_1657_cov_5.723471"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..1179
                     /locus_tag="PLI16_10315"
     CDS             166..1179
                     /locus_tag="PLI16_10315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOH84993.1"
                     /translation="MPEFAELPEEEKEFYEHYRFIVDPGQQPMRIDKFLSDRISNVSR
                     SKIQSAADANAIQVNDKPVKSSYKIKPRDIITIVLPFPPHEFELIAEDIPINIVYEDN
                     DILVINKDAGMVVHPGYGNFTGTLLNALLFHLKDDADSQPLLVHRIDKDTSGILVVAK
                     NELSQAKLSKYFFDHSIDRRYVALVWGDFENDEGTITGYIGRNLRNRKMMDIYQDETQ
                     GRWAVTHFRVLERFRYVSLIECTLETGRTHQIRAHMKHIGHPLFNDALYGGDIIMKGT
                     TFSKYKQFVQNCFQLMPRQALHAQSLGFNHPSTGKYINFTSEITQDMRLVIEKWQHYV
                     QQL"
     gene            complement(1199..>1657)
                     /locus_tag="PLI16_10320"
     CDS             complement(1199..>1657)
                     /locus_tag="PLI16_10320"
                     /inference="COORDINATES: protein motif:HMM:NF039584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HOH84994.1"
                     /translation="GLFSNATMLYGHQELYSHRLHHMELLRNLQDETNGFNAFIPLKF
                     KNKNNAMSYLPEVTLVEDLRNYAVSRVFLDNIPHLKAYWPMLGKKQAQLALGFGVDDF
                     DGTIDDTTEIYVAAGMEKSENTMNVQEIKQIITRAGFVPVERYSDYTARI"
BASE COUNT          479 a          383 c          336 g          459 t
ORIGIN      
        1 aatcccaact aaagcaatag attgatgaat catttggaca agttcgatta cctcttttgc
       61 ttaaattggg attattttgt atagctttgt actattgatt tatcagagtg ctttcacgac
      121 cggtaaatca tcagataagt ttttggtatt tgttaaagct atagtatgcc agaatttgca
      181 gaactgcccg aagaagaaaa agaattttac gagcattacc gttttattgt tgatcccggg
      241 caacagccca tgaggattga caagtttttg tccgacagga tatccaatgt ttcgagaagc
      301 aagatacaaa gcgctgccga tgccaatgcc atccaggtca acgataaacc cgtcaagtca
      361 agttataaga ttaagccccg cgatattata actatcgttt taccctttcc accccatgag
      421 tttgaactta ttgccgaaga tattcccata aatatcgttt atgaagacaa cgacatcctt
      481 gttatcaata aggatgcagg tatggtagtg catccgggct acggaaattt taccggaacg
      541 ttgttaaatg cgttgctttt tcatctgaaa gacgatgctg attcgcaacc cttactggtg
      601 caccgtattg ataaggacac atcgggcata ttggtggtag caaaaaatga actaagccaa
      661 gccaaacttt ccaagtattt tttcgatcat tccatcgacc gcaggtatgt agcgttggtc
      721 tggggcgact ttgaaaatga cgaaggtact ataaccggct atatcgggcg caaccttcgc
      781 aaccggaaga tgatggacat atatcaggat gagacccagg gccgatgggc ggtaacccac
      841 ttcagggttc ttgagcggtt ccgctatgtt tcgctgatag aatgcaccct cgaaacaggc
      901 cgcacgcacc agatacgtgc gcacatgaaa catatcggcc atccgctgtt caatgatgca
      961 ctttatgggg gcgacattat aatgaaaggc actacctttt caaaatacaa acaatttgtt
     1021 cagaattgtt ttcaactgat gccgagacag gccctgcacg cgcaatctct gggctttaat
     1081 cacccgtcaa ccggcaagta cataaatttt acctctgaaa ttacgcaaga tatgcggctt
     1141 gtcatcgaaa aatggcaaca ttacgttcag caactctaaa aattaaggcc aaacaaaatc
     1201 aaatcctcgc tgtgtaatcc gaataccttt ccaccggaac aaaacctgcc cgggtaatta
     1261 tctgttttat ttcctgtaca ttcatggtgt tttctgattt ttccataccg gcagcaacat
     1321 atatttctgt tgtatcatca atagtgccgt cgaaatcatc cacgccgaaa cccagagcca
     1381 gttgcgcctg tttttttccc agcataggcc aataggcttt caggtgcggg atgttatcaa
     1441 gaaataccct gctcactgca taattcctca aatcctccac cagcgtcacc tcaggcaagt
     1501 acgacatcgc attgtttttg tttttaaact ttaacggaat aaacgcgtta aagccattag
     1561 tctcatcctg caaattcctc aacaactcca tatggtgcag ccggtggcta tacagctcct
     1621 gatgcccgta caacatcgtg gcgttggaaa aaaggcc
//
LOCUS       DAOSFP010000434         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_76283_length_1645_cov_4.449686, whole genome shotgun sequence.
ACCESSION   DAOSFP010000434 DAOSFP010000000
VERSION     DAOSFP010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76283_length_1645_cov_4.449686"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(541..>1645)
                     /locus_tag="PLI16_10325"
     CDS             complement(541..>1645)
                     /locus_tag="PLI16_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOH84995.1"
                     /translation="AADYIVKQFEKSGVKPFEPGYLQPFEMCINTFPGEIKLVANGRE
                     LIPGTDFVVFPDAAPTNAVFKTMWLNGESLNTPQKIKKFNAARLGNVALIIDTGFKDL
                     KNEKLNQAAALITITDKNVSWHVSRAQQQSRQIKISLKRPSLPEKTRKIYLKIDAALQ
                     KNYKTNNVVGYLPGRVSSDTFLVVGGHYDHLGMMGNKTFFPGANDNASGTAIVLDLAR
                     HFSDTANRPRYSVAFVLFAAEEAGLLGSEYFTTHPPVPLKNIKFMVNLDMVSTGSEGI
                     KVVNGSVLKEEFEKLKSINNHHNYLKTISERGEAANSDHYFFYKNGVKSFFIYSLGDE
                     YKEYHTVTDAAAGLPLTKYNEIFKLVKEFIYSF"
BASE COUNT          448 a          342 c          329 g          526 t
ORIGIN      
        1 tttatgaaac ttagaatata ttgaaataca gttagtttta ttaagttaga tggaattacg
       61 cattaaatac ttttatttct acgcaataga actaatcaat cattatcttc taataatcaa
      121 taacatatga tttgattcgt taaagcgtaa tcgacaaaga aaatgtaata ccgccatagc
      181 ggaaacattt tctaagtcgg ggctaccccc cgcaacttcg ttcacggggc aggcaaatac
      241 agcccacgat gccaccactg cgcaatagtc cactacttcc tattgctcaa tctgggatta
      301 gcagacacac cggagttatg gtaaatatct gagtaataaa aaaaagaacc ccggggccgg
      361 ggttctttcg gaactttttt ttgcagctga ggatttgggg gataaaatcc tttcgcttat
      421 tgaggggttt aatgcatatt agacaacgca aatgtccatt tggttgcctg tgattgtttt
      481 ttttatctta tcatagcaaa tactttaacg caagccttta tagggtctgc caggttcaga
      541 ttaaaaacta taaataaact ccttcaccag cttaaaaatc tcgttatatt ttgtcagggg
      601 caggccggca gctgcatcag taactgtatg atattcctta tattcgtcgc caagagaata
      661 aataaaaaaa ctttttacac cattcttata aaaaaagtag tgatcgctgt tagcggcttc
      721 tcccctttcg ctgattgttt tcagataatt atggtggttg ttgatggatt ttagtttttc
      781 aaactcttct ttcagcacgc tgccgtttac cactttaatt ccctcgctgc ccgtactaac
      841 catatcaaga ttcaccatga atttaatgtt ctttaaaggc accggggggt gtgtggtaaa
      901 gtattctgag cccagcaatc ctgcctcttc agcagcaaac agcacaaaag ccactgaata
      961 tcgcggcctg ttggctgtat ctgaaaaatg cctggccaaa tcgagcacta tcgctgttcc
     1021 gctggcatta tcattggcgc cgggaaaaaa agttttattt cccatcatgc caagatggtc
     1081 gtagtgtccg ccaacaacaa gaaatgtgtc ggaactcaca cgcccgggca ggtagccaac
     1141 tacgttgttg gttttatagt ttttttgcag cgcggcgtca attttcaaat atatttttcg
     1201 tgttttttcg ggtagtgacg gccttttcag gctgattttt atttgccggc tctgttgctg
     1261 tgcacgggaa acatgccacg aaacattttt gtcggtaata gttatcaaag cagccgcctg
     1321 gtttaatttt tcatttttca ggtctttaaa tcccgtatca attatcagcg ctacatttcc
     1381 caatctggca gcgttgaatt tttttatttt ttgcggtgtg tttaaacttt ctccgttaag
     1441 ccacatggtt ttaaaaacgg cattggttgg cgcagcatca ggaaaaacca caaaatcggt
     1501 cccgggtatt agttccctgc cattggccac aagtttaatt tcgccgggaa aggtgttgat
     1561 acacatttcg aagggttgca aatatccggg ttcaaaaggt tttacacctg acttttcgaa
     1621 ttgttttacg atataatctg cggct
//
LOCUS       DAOSFP010000435         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_78469_length_1615_cov_3.164103, whole genome shotgun sequence.
ACCESSION   DAOSFP010000435 DAOSFP010000000
VERSION     DAOSFP010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78469_length_1615_cov_3.164103"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..>1615
                     /locus_tag="PLI16_10330"
     CDS             60..>1615
                     /locus_tag="PLI16_10330"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOH84996.1"
                     /translation="MKQYKFLNNISGWIVFLIASTVYILTSEPTASYWDCGEYISTAY
                     KLQVGHPPGAPLFQLLGRFFSLFAFGDVTLVARMINTMSALASGFTILFLFWTITALT
                     KKIVFKSENSSLQEIITVLGAGFVGAMAYTFSDSFWFSAVEGEVYATSSFFTAIVFWA
                     ILKWEEVADEPYANRWLVFIAFMTGLSIGVHLLNLLCIPAMTMVYYYRKIKHSKKNIT
                     IALVLSLLILGFVQSGIIPWTVKLAGYFELFFVNNAGMPFNSGIIIYLTPLILFIVLG
                     LVFSKKRGKTILNTIVLALAFLLLGYSSFLVLVIRSNANTPINENAPKDAISLLRYLN
                     REQYGDWPLLYGPYFNAPYDSDEPYVDGKPVYARDEAQKKYVIVDESKLSENNYDKRF
                     YTFFPRMWSRSSDLHTRGYQTWSKMDGTPITVTYRNGSQEVINKPTFFENLRFFFTYQ
                     VGHMYLRYFMWNFSGRQNDQQGYGGLLNGNWMTGIKTIDKIRLGPQDNMPETYASTKG
                     RNYFYLLPLL"
BASE COUNT          427 a          393 c          342 g          453 t
ORIGIN      
        1 taataccgac atatccctga aggtttttta cttaatttcg cactgcaaaa aagcccatta
       61 tgaagcaata taaattttta aacaatattt caggctggat agttttttta attgcttcta
      121 ccgtttacat tctcacatca gagccaacag ccagctattg ggattgcggc gagtacattt
      181 ccacagcata taaactgcag gtggggcatc ctcccggagc acccctgttt cagcttctgg
      241 gacggttctt ctccctgttt gccttcggtg atgttacgtt ggtggcccgc atgatcaaca
      301 cgatgtcggc cctggctagc ggctttacaa tactattttt gttttggacc attacggccc
      361 tgacaaaaaa aatcgttttc aaatccgaaa acagtagtct tcaggaaata atcaccgtgc
      421 ttggtgcagg ctttgttggt gcgatggctt acactttttc cgattcgttt tggttttcgg
      481 ctgtcgaagg cgaagtatat gccacatctt cgtttttcac tgccatagtc ttttgggcca
      541 tactaaagtg ggaggaagtg gccgatgaac cctacgccaa ccgatggctc gttttcattg
      601 cctttatgac aggcttatcc ataggcgtcc acctgctcaa cctgctgtgc attccggcta
      661 tgaccatggt atattactac agaaaaatta aacattccaa aaaaaacata accatcgcat
      721 tagtgctttc cttgctaatc cttggatttg tgcaatccgg aataataccc tggaccgtaa
      781 aactggccgg atactttgaa ctgttttttg tcaataacgc cgggatgcct tttaactcgg
      841 gcattataat ctaccttacc ccgcttatcc tgtttatcgt gctcggcctg gtgttctcaa
      901 aaaaaagagg aaaaaccatt ctgaatacta tcgtgctggc cctggcattt cttttattag
      961 gatattcttc tttcctggtg ctggtaatcc gttcaaatgc caacaccccg ataaatgaaa
     1021 atgcgcccaa agacgccatc agcctgctga gatacctcaa ccgcgaacaa tatggtgatt
     1081 ggcctttatt gtacggaccc tatttcaacg caccctacga cagcgacgag ccttatgttg
     1141 acggcaagcc cgtttatgcc cgtgatgagg cgcaaaaaaa atatgtcatc gttgatgaaa
     1201 gcaaactcag cgagaacaat tacgacaaga ggttttatac gttctttccg cgtatgtgga
     1261 gccgcagctc cgacctgcac accaggggat accaaacgtg gagcaaaatg gacggcacac
     1321 cgataacggt aacgtacaga aatggctcgc aggaggttat caacaaacct actttttttg
     1381 aaaacctgag gtttttcttt acttaccagg taggccacat gtatcttcgc tactttatgt
     1441 ggaacttttc cggacgccag aacgaccagc agggatacgg cggcctgcta aacggaaact
     1501 ggatgactgg catcaaaacc atcgacaaaa tcaggttggg ccctcaggac aacatgcccg
     1561 aaacttatgc cagcactaaa ggacgaaatt atttctattt gctgccgctg ctgtt
//
LOCUS       DAOSFP010000436         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_78716_length_1612_cov_2.392421, whole genome shotgun sequence.
ACCESSION   DAOSFP010000436 DAOSFP010000000
VERSION     DAOSFP010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78716_length_1612_cov_2.392421"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..913)
                     /locus_tag="PLI16_10335"
     CDS             complement(302..913)
                     /locus_tag="PLI16_10335"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOH84997.1"
                     /translation="MKTDNKILMQMAKESLKGKWGLAIGTFVIYALIVGGVGSIPFAG
                     APGSLIVGGPLLLGLTMFTLNISRNTNPKLEQLFHGFRNFGTALGTYLLMVLFITLWS
                     LLLIVPGIIAAISYAMTFFILADDTTIAPMDALRKSKKMMYGYKKKFFYLVLRFFGWF
                     LLCILTLGIGFLWLYPYMQVSFAKFYEDIKDNPISAEDTTPAA"
     gene            complement(1150..>1612)
                     /locus_tag="PLI16_10340"
     CDS             complement(1150..>1612)
                     /locus_tag="PLI16_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH84998.1"
                     /translation="GSLYTIDDFGEGVRLDLKNIILPAFSLGIRPLAVIIELTRSSML
                     EVLSQDYIRTAKAKGLSFFMVIKRHALKNVMNPVVTAISGWFASLMAGAVFVEYVFDW
                     KGMGMVMVDGLNKYDFPVVMGSLLFIAVILVIINILTDITYGLLDPRVRLN"
BASE COUNT          499 a          346 c          332 g          435 t
ORIGIN      
        1 caaaacaaca attatctata ccgaaatcaa cgaaagcaag gctgttgaac tcctgcaaat
       61 ggatcatcat tatggcattg cggagtgttt tacagtaaaa gatatcaata ataaggatcg
      121 gtttttcttc ggtaaggtta gctcttaaca aacatcatgg caacgcgggc cggtttgtac
      181 aaaacggcta tagcaatgga gtaggagcgt gttatgagca aactgtctca aaacgccctt
      241 tttgctgatt ttcactgcct tcttttcccc gccctgatga caagtagttg ttccccgaga
      301 cttaggcagc aggtgttgta tcttcggctg atatggggtt gtcttttatg tcctcataaa
      361 actttgcaaa actgacctgc atgtatggat aaagccatag gaaacctata cccagtgtca
      421 ggatacaaag taaaaaccaa ccgaaaaacc ggagcaccag gtaaaaaaac ttctttttat
      481 atccatacat cattttttta cttttccgga gggcatccat gggtgcaatt gtagtatcat
      541 cggccagtat aaaaaaggtc atggcatacg agatggctgc aatgatgccc ggcacaatga
      601 gcaacagcga ccagagagtt atgaataaaa ccatcagcag ataggtgccc agggctgtcc
      661 cgaaatttcg gaacccatga aataattgtt ccagtttagg gtttgtgtta cgcgaaatat
      721 tcagggtaaa catggtgagc ccaagcagca gagggccccc cactatcagt gaacccggcg
      781 ccccggcaaa agggatggat ccgacgcccc ctactattaa agcataaatc acaaaagttc
      841 ctatggccaa tccccatttt cctttcaggg attctttggc catttgcatt aaaatcttgt
      901 tgtctgtttt cattttttta ttgattttgt gagctttatg gtaattatct tatattttta
      961 aattccataa caaatatagt aaaataaaat gaaaaataca agtgttgatt attccggtgt
     1021 gcagtaacaa aaaaagtcac tattgaatga taacataaga aagcgcatta agggcacctc
     1081 taaattatat tgatagggaa aatctaacct gaaaaatata tatgaatttc gaaatttttc
     1141 aaggtgccct tagttcaatc tcaccctggg gtcaagcagc ccgtaagtga tgtcggtaag
     1201 aatattgata atcaccagga tgactgcgat aaaaagcagc gagcccatta ccacagggaa
     1261 atcgtatttg ttcagcccgt caaccatcac cattcccatg cctttccagt cgaacacata
     1321 ttcaacaaag acggctcctg ccatcagcga ggcaaaccat cccgaaatag ctgttaccac
     1381 aggattcatc acatttttaa gggcatggcg ttttattacc ataaaaaacg acagaccttt
     1441 ggctttggcg gtacgtatat aatcctgcga gagcacttcc agcatcgaac tgcgtgtgag
     1501 ttcgatgatg actgccaatg gcctgatgcc aaggctaaag gccggaagaa taatgttttt
     1561 tagatcgagg cgcacacctt cgccaaagtc atcaatagta tagaggctgc ct
//
LOCUS       DAOSFP010000437         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_81312_length_1578_cov_5.294156, whole genome shotgun sequence.
ACCESSION   DAOSFP010000437 DAOSFP010000000
VERSION     DAOSFP010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81312_length_1578_cov_5.294156"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="PLI16_10345"
     CDS             <1..975
                     /locus_tag="PLI16_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOH84999.1"
                     /translation="FENIHVLEAQAIPDGNFPTIKSPNPEEQAALSMAIEKAKETGAE
                     LVLATDPDADRVGIAVKNNKGEFVLLNGNQTGSLLIFYLLSQWKNFGRLTGNEFIVKT
                     IVTSELLSEIARKFDVECFDVLTGFKYIAEIIKKYEHKKRFIGGGEESYGYLVSDFVR
                     DKDAVISCCMIAEAAVFAKEQGLSLFDLLIGIYHEFGFYYESLLSVTKQGKSGSEEIK
                     KMMEKFRNNPPKSINNSEVVMIKDYLLQQQYGISANEIIPIDLPKSDVLQFFTSDGSK
                     ITVRPSGTEPKIKFYFGIIAELPDGAHYEEAEQKCKGKIDNIKAGLGL"
     gene            complement(1041..>1578)
                     /locus_tag="PLI16_10350"
     CDS             complement(1041..>1578)
                     /locus_tag="PLI16_10350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH85000.1"
                     /translation="AAHAITPADSLPAYITLGLTTPYSVAAGTAVCATIAMTVNIAAN
                     DTIFVGSDDDFVANAAVAGAAYLNVNNTWGWYSITGLVPIADLITYNPTTGINETETD
                     INFSVYPNPASEVLNVVSESNMISMKVMNALGQEVVSKTPDSKKITINTSDFISGIYF
                     VQVQTTTGMSTRKIIISK"
BASE COUNT          490 a          301 c          351 g          436 t
ORIGIN      
        1 tttgaaaata ttcatgtact cgaggcacag gcaatccccg acggcaactt cccgaccatc
       61 aaatccccga atcctgaaga gcaggcagcc ctaagcatgg cgattgaaaa agccaaagaa
      121 acgggcgccg aacttgttct cgctaccgac cctgatgccg accgtgtggg tattgccgta
      181 aaaaataata aaggcgaatt tgtgctgctc aacggcaatc agaccgggtc gttgctgata
      241 ttctacttgc tgagccagtg gaaaaatttt ggcaggctga caggaaatga attcatagta
      301 aaaactatag ttaccagcga gctgttatca gaaatagccc ggaaatttga tgtggagtgt
      361 tttgatgtgc ttaccggttt taaatatatt gccgaaatca tcaaaaagta cgaacataaa
      421 aaacgtttta taggcggtgg tgaagaaagt tacggttacc tcgtgagtga ttttgtccgt
      481 gataaagacg ccgtgatatc ctgttgtatg attgccgaag cggcggtttt tgccaaagag
      541 caggggctct cgctttttga cctgctgatt ggtatttatc atgagtttgg tttttattac
      601 gaaagcttgc tctcagttac caaacaaggc aaatccggct ccgaagagat aaaaaagatg
      661 atggaaaaat tcagaaacaa cccgccaaaa tcaattaata attcagaagt tgtgatgata
      721 aaagattatt tattgcagca acaatatgga atttcagcca acgaaatcat ccctattgac
      781 cttccgaaat ctgatgtgtt gcaatttttc accagcgatg gtagcaaaat tacggtgcgt
      841 ccgtcaggca ctgaacctaa aattaaattc tattttggta ttattgcgga attacctgat
      901 ggtgcacatt atgaagaagc cgaacaaaaa tgtaagggaa aaattgataa tattaaagcc
      961 ggtctggggc tttgatacct gaataaaaaa gaaaggctgc ccaacagagc aaccttgcta
     1021 acaaagattc acttttatta ttacttcgag ataattattt tcctggtaga cattcctgta
     1081 gtcgtttgaa cctgtacaaa ataaattcct gaaataaaat ccgaagtatt gatagttatt
     1141 tttttgctat caggagtttt gcttacaacc tcctggccta aggcgttcat gaccttcatt
     1201 gaaatcatat tgctttctga aacaacgtta agtacttcgc tggcagggtt aggataaacg
     1261 ctgaaattga tgtcagtttc agtttcattg attccagtgg taggattgta ggtgataagg
     1321 tctgcaatag gtacgaggcc tgtaatagaa taccagcccc atgtgttgtt tacattaaga
     1381 taggcggcac ctgccactgc tgcattggca acaaaatcat catctgaacc gacaaaaata
     1441 gtatcattag cagctatatt cacggtcatg gcaatagtag cgcaaacagc agtacctgct
     1501 gcaacactgt atggggttgt caggccaaga gtaatatatg cgggtaatga gtcggcagga
     1561 gttattgcat gagcagcg
//
LOCUS       DAOSFP010000438         1578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_81360_length_1578_cov_2.250164, whole genome shotgun sequence.
ACCESSION   DAOSFP010000438 DAOSFP010000000
VERSION     DAOSFP010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81360_length_1578_cov_2.250164"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLI16_10355"
     CDS             <1..270
                     /locus_tag="PLI16_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOH85001.1"
                     /translation="QKILLYKILRKKGVPRKILKRPKQGFTGPDGYYKNIDFYRNYLA
                     ESKFAALGAIQQEYLNKLLAEGDFWRLWKITIMELWFRKWKPVLG"
     gene            complement(276..1538)
                     /locus_tag="PLI16_10360"
     CDS             complement(276..1538)
                     /locus_tag="PLI16_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOH85002.1"
                     /translation="MQQAKHHDSETELLRFTTAGSVDDGKSTLIGRLLFDCGLIPDDQ
                     YESIKLASERKGEAEVNLALLTDGLRAEREQCITIDVAYRYFATKNRRFIIADTPGHI
                     QYTRNMVTGASTANLALVLVDARKGIIEQTRRHIFIASLLKIPHLLLCVNKMDLVAYN
                     KEVFEAIKSEFSIFSAKLEISDIQLIPVSALRGDNIVTKSENMPWYKGPSLLYILENV
                     HIGSDYNLIDCRFPVQYVIRPYNSDYRAYAGMVAAGIFKSGDKVMHLPSKLITSVSSI
                     EIANEALTEACPPMSVCIQLKDHIDISRGDMLVRENNSPTVSSQLEVMVCWFSDKPLT
                     EKNRFILKHTTREVKVVVEEIRYKIDINTLHRIENTCQLTLNEFARISIRTSQPLFFD
                     KYKLNRATGSLIFIDEKTFETVGAGMIL"
BASE COUNT          415 a          324 c          354 g          485 t
ORIGIN      
        1 caaaagatac tgttgtataa aattctccgg aaaaaaggtg tcccgcgcaa aatcctgaag
       61 cggccgaaac agggttttac cgggcctgac gggtattata aaaatatcga tttttatcgc
      121 aattatcttg cagaaagcaa atttgcagcc cttggagcaa ttcagcagga gtacctgaac
      181 aagttgcttg ccgaaggaga tttctggaga ttgtggaaaa taactatcat ggaattatgg
      241 tttcggaagt ggaagcctgt cctggggtga gttttttata gaatcatacc ggcgccaaca
      301 gtttcaaaag ttttttcatc aataaaaatc aggctgcctg tcgcgcggtt cagtttgtat
      361 ttgtcgaaaa acagaggttg agaggttctg atgctgatcc ttgcgaattc gtttaatgtc
      421 agttgacagg tgttttcaat gcgatgaagg gtgttgatat cgattttgta tctgatttct
      481 tccactacaa cttttacttc acgggtagta tgttttagta tgaaccggtt tttttctgtc
      541 aatggcttgt cggagaacca gcaaaccata acttccagtt ggctcgaaac agtcggagaa
      601 ttgttttccc gtacgagcat atctccacgg ctgatgtcga tatgatcttt caattgaatg
      661 caaactgaca ttggagggca tgcctcagta agagcttcat tggcaatttc tattgaacta
      721 acgctggtta tgagctttga tggcaggtgc attactttat caccggattt aaaaatgcct
      781 gctgcgacca tccctgcata agctctgtaa tcggaattgt agggcctgat tacatactgc
      841 accggaaacc ggcagtcaat caggttatag tcgctgccga tatgcacatt ctcgaggatg
      901 tacaacagag agggcccttt gtaccatggc atattttccg attttgtaac aatattatct
      961 cctctgagcg cagatacggg aatcaattga atgtcgctga tttccagttt tgccgaaaaa
     1021 atcgaaaact ctgactttat tgcttcgaat acttctttgt tgtaggctac caaatccatt
     1081 ttattgacac acaataataa gtgtggtatt ttcagcaatg aggcgatgaa aatatgcctg
     1141 cgtgtttgtt caataattcc ttttcgtgca tcaacaagca cgagcgcaag gttagccgtg
     1201 gatgcgcccg taaccatatt tctggtgtat tgaatgtgcc cgggtgtgtc tgcaatgata
     1261 aagcgacggt ttttcgttgc aaaatagcgg tatgccacat caatagtgat gcattgttcc
     1321 ctttcggcac gcaggccatc ggtaagcagg gcgagattta cttcggcttc acccttacgt
     1381 tcactggcca acttaatact ttcgtactga tcgtcaggta ttaacccaca atcgaacaga
     1441 agtcgtccga taagtgtgct tttaccatca tcaacgctac cggcggttgt aaaccgcagc
     1501 aattccgttt cgctatcatg atgttttgct tgttgcatgt taaaagtaac cttctttttt
     1561 acggtcttcc atagctgt
//
LOCUS       DAOSFP010000439         1558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_82911_length_1558_cov_4.021291, whole genome shotgun sequence.
ACCESSION   DAOSFP010000439 DAOSFP010000000
VERSION     DAOSFP010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82911_length_1558_cov_4.021291"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PLI16_10365"
     CDS             complement(<1..1003)
                     /locus_tag="PLI16_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HOH85003.1"
                     /translation="MLALLFVYCAHTGYSSVPGNKGTILTIKENTYNKLVLVNSLSLQ
                     DIRTIKVNTAKGLFSEFIIPEYGASNAIGNPKLPVMKKLIEVPVGATVKINTLHSTYT
                     DITLNDLGALFPVMPAQPSLSKSDDPKKADFKYNKAAYKLNQFLPHELATLTVLGTMR
                     GTKLGRLEIAPLYYNPAKKTLRIYTSVEIEIVFENANVAATIQQKENFFSPYFAPSYS
                     KIFNYKPLEHKANFTKYPVKYVIVADTMFYSSLQPFVQWKTKKGFTVVQAYTNNPAVG
                     NTTTSIKSYLQNLYNAGTAADPAPSFVLLVGDVAQVPSFAGTEGSHVSDLYYCEYTGD
                     "
     gene            complement(1153..>1558)
                     /locus_tag="PLI16_10370"
     CDS             complement(1153..>1558)
                     /locus_tag="PLI16_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOH85004.1"
                     /translation="APEVTEKTVEAINKNYYDFVCLNFANADMVGHTGIYPAIIKALE
                     TVDLCVEKVITAALNNQYSVILTADHGNSDMAENPDGSPNTAHTCNPVPCFLFDPDNK
                     TIRNGKLADIAPTILTILDLPVPEEMTGEILV"
BASE COUNT          412 a          289 c          386 g          471 t
ORIGIN      
        1 aatctccggt atattcacaa taataaaggt cggaaacatg ggatccttct gtgccggcga
       61 acgagggtac ctgcgccaca tcccctacca gcaaaacaaa ggatggtgca ggatcggcgg
      121 ctgtgccggc gttgtatagg ttttgcagat aggattttat cgaagtagtg gtgttgccca
      181 cggcagggtt gttggtatag gcctgtacca cggtaaatcc ttttttggtt ttccattgca
      241 caaagggctg caacgaagag taaaacatcg tgtcggccac aatgacatat tttacaggat
      301 attttgtaaa attggctttg tgttccaggg gcttgtagtt gaagattttg ctataagaag
      361 gagcaaaata gggcgaaaag aaattttctt tttgttgtat ggtggctgca acattggcgt
      421 tttcgaacac tatttctatt tccactgagg tgtatatccg taatgttttt tttgccggat
      481 tgtaatacag cggtgcaatc tccagccggc ccagcttagt gccgcgcatg gttcccagga
      541 cggttagcgt agctaattca tgcgggagaa actggtttaa tttgtaagct gccttattgt
      601 atttaaaatc ggctttttta gggtcatcgc ttttggatag tgaaggctga gcaggcataa
      661 caggaaaaag cgcacccagg tcattgagcg tgatatcggt ataggtacta tgcaacgtgt
      721 tgattttcac cgtggcgcca accggcacct ctataagttt tttcattacc ggcagtttgg
      781 gattaccgat cgcatttgac gctccgtatt cggggataat aaattcggag aacaatcctt
      841 ttgcggtgtt cacttttatg gtacgaatat cctgtaagga cagcgaatta accaaaacaa
      901 gtttgttgta agtgttttct ttgattgtca ggatggtgcc tttgttgccg gggacggatg
      961 aatagccggt atgcgcgcaa tacacaaata ataatgcaag catacatagt ttgtaaaaag
     1021 cggggtaatt tttcataaaa ttgggataaa atagtgtcag ataattttat gtaaaggtaa
     1081 ataaatcttt tttatttgag aaatttaata aaaaaaatca ctttacacct ttataatcag
     1141 aaaacaggta gtttaaacca gtatttcgcc ggtcatttct tcaggtaccg gcaggtcgag
     1201 aatagtcagt atagtggggg caatatcagc cagttttccg ttgcgtatag ttttattatc
     1261 cgggtcgaac aggaagcagg gtaccgggtt gcaggtatgg gccgtgttgg gcgagccatc
     1321 gggattttcg gccatatccg agttgccgtg atcggcggta aggatgaccg aatactggtt
     1381 gtttaaagca gccgtgataa ctttttctac gcataggtcc acagtttcca acgcttttat
     1441 gatagccgga taaattcctg tatgccccac catgtcggca ttggcaaagt tcagacaaac
     1501 aaaatcataa tagtttttat tgattgcttc cacggttttt tcggtaactt ccggggcg
//
LOCUS       DAOSFP010000440         1549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_83694_length_1549_cov_4.146586, whole genome shotgun sequence.
ACCESSION   DAOSFP010000440 DAOSFP010000000
VERSION     DAOSFP010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83694_length_1549_cov_4.146586"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1420
                     /locus_tag="PLI16_10375"
     CDS             80..1420
                     /locus_tag="PLI16_10375"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOH85005.1"
                     /translation="MAPIKININRISKFVDESNLKTLQPEVDKHYKSLTEKTGAGNEF
                     LGWLTLPDETLNSSLIQRINDEAQRLRALSEVVLVIGIGGSYLGARAIIDALNTQFQS
                     YRKGNPEIIYAGQNISEDYLFDLIELLNQREYSIIIISKSGTTTEPALAFRLLKNHLE
                     NKYGKENAKTRIVAITDKSRGALKKLADKEGYPQFVIPDDVGGRFSVLTPVGLLPVAV
                     AGFDISQLVSGASSMQRELYKTSALDTNPACRYAAVRNALYRKGIKVEMLVNFQPSLF
                     YITEWWKQLYGESEGKDHKGIFPAGANFSTDLHSMGQYIQDGLRILFETMLLVEKPGR
                     NLTVPFEEDDADGLNYLAGKRIHEVNKMAAAGTAIAHNDGGVPVMSISLTELNEFTLG
                     ELIYFFEFACGISGYMLEVNPFDQPGVEAYKNNMFALLGKAGYEDKQKELLKKL"
BASE COUNT          488 a          339 c          339 g          383 t
ORIGIN      
        1 cgtaattcaa tatgttataa aagcatacac cacctttttt attttaattt tataatttca
       61 caccaaaatt ataaaaaata tggcaccaat aaaaattaat attaacagga ttagcaaatt
      121 cgttgacgaa agcaacctga aaacccttca gccggaagtt gacaaacact acaagtcatt
      181 gacagagaag actggtgctg gcaacgaatt tctgggctgg ctcaccctgc ctgacgaaac
      241 tctgaattca tcattgatac aaagaattaa cgacgaagcc caacgtctca gagccttgtc
      301 ggaagtagtg ctggtaatag gcatcggcgg ttcgtatctg ggagcgcggg ccattattga
      361 tgcgcttaat actcaattcc aaagctatcg caaggggaat cctgaaataa tttacgcagg
      421 tcaaaacatc agcgaagact atcttttcga cctgatagag cttttaaacc aacgcgagta
      481 cagtattatc atcatttcaa aatcaggaac caccaccgaa ccggccttag cgttccgcct
      541 gctgaaaaat cacctggaaa acaaatatgg taaagaaaat gccaaaaccc gaatcgtggc
      601 tatcaccgac aaaagcagag gagctttgaa aaaattggca gacaaggaag gttatccgca
      661 gtttgttatc cccgatgatg tggggggaag gttttcggtc ttaacacccg tcggactttt
      721 acccgtagct gtggcgggct ttgacatatc ccaactcgta tcgggcgcct cttccatgca
      781 aagggaacta tataaaacct ccgctctgga caccaaccct gcctgtcggt atgctgccgt
      841 gcgcaatgct ctgtaccgca aaggaataaa ggtagaaatg ctggtaaatt ttcaaccatc
      901 cctgttttat attaccgaat ggtggaaaca actttatggc gaaagcgaag gcaaagatca
      961 taaaggaata ttcccagccg gcgccaattt cagcaccgac cttcattcca tggggcaata
     1021 tatacaggac gggctgcgca tactcttcga aacgatgctc cttgtggaaa aacccggccg
     1081 caatctgact gttccttttg aagaggatga tgccgacggg ttaaactacc tggcaggaaa
     1141 acgcatacat gaagtaaaca aaatggccgc ggccggaaca gctatcgccc ataatgacgg
     1201 cggcgtaccg gtaatgagta tcagcctgac agaactgaat gaatttaccc tgggtgaact
     1261 gatctacttc tttgagtttg cctgcggtat cagcggttat atgcttgagg taaatccatt
     1321 cgaccagccg ggcgtggaag cctataagaa taatatgttt gccttattgg gcaaggctgg
     1381 ctatgaagac aaacaaaaag agcttttaaa aaaattgtag gaaattgttt tataaaaatg
     1441 ccattttccg gatgacggca ttttattaac aaacaaaatt caggtgcgaa gtcaatgaat
     1501 ttgcacaata gttattgttt gaaaaacatc agcatatcgg cttcaaggg
//
LOCUS       DAOSFP010000441         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_84316_length_1542_cov_3.773369, whole genome shotgun sequence.
ACCESSION   DAOSFP010000441 DAOSFP010000000
VERSION     DAOSFP010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84316_length_1542_cov_3.773369"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..>1542
                     /locus_tag="PLI16_10380"
     CDS             279..>1542
                     /locus_tag="PLI16_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOH85006.1"
                     /translation="MNLQEFKSKHSLLKEWQYTLKPIEKGYNMRTLYVNVGTHEIKEK
                     PVSQMMKEKFTGGKGFGLKLLWDATKPETKWNDPENEIVINTGPICGITQYSGSGKSL
                     VVAISPQTHIPIDSNVGGYFGPFMKFAGFDSMEIQGKSDKEVIVYIDGVNGKVEIYDA
                     NDLPIDSHVIGEVLHEVFADDEKDRMNVSVVSAGTAADYSLIGCLNFTFFDMKRKVVR
                     LKQAGRGGLGTVLRDKKIKAVVAKVPGVKGNLNNVVDLEAIMERGKRFNKEMRELDDS
                     QCQMRKIGTAHLTHIMNDYDLFPTHNFKFGSHPDAIKVHAERYKERFTQNMPDGCWIG
                     CNMSCAKGVDNYELRTGPYKGSKVIVDGPEYETAASLGSCAGIFNPEWTIEANFYCDT
                     YGICTISWGTILGFVMECYENGIINEERT"
BASE COUNT          481 a          348 c          337 g          376 t
ORIGIN      
        1 acctggccga catgaacccc atcgaagcca tggaatttct gaaagagaaa atgaaattta
       61 ctcaaaccaa cgaagagttc ctgctcttta tgaataccta agcacttcgg taacccatca
      121 aaaaactcat accttttcta tcacggtatg agtttttttt gtgcccgttt tttcgatatg
      181 catatcgcta catatcaata tttttaatgc tttccgcttg ttttgtacct gaaaagaaac
      241 tatttttgta aacacaaata tttataaaaa cacgaatcat gaatcttcag gaatttaaaa
      301 gcaaacacag cctgttaaag gaatggcaat acaccttgaa acccattgaa aaaggctata
      361 acatgcgtac cctgtacgtg aacgtgggca cccatgaaat aaaagaaaag ccagtttcgc
      421 aaatgatgaa agaaaaattc accgggggaa aaggtttcgg tctgaaacta ttatgggatg
      481 ccaccaaacc tgaaacaaaa tggaacgacc ctgaaaatga aattgtgatt aataccggcc
      541 ctatatgcgg tatcacccaa tactccggct ccggaaaatc gctggtcgtg gctatttcgc
      601 cgcagactca catacccatt gacagcaatg tcggcggtta ttttggacct ttcatgaaat
      661 ttgccggctt tgactccatg gagattcagg gcaaatccga caaagaggtc atcgtatata
      721 ttgatggcgt gaacggaaaa gttgaaatct atgatgccaa cgacctgccc atcgacagcc
      781 atgttatcgg cgaggtgctt catgaggttt ttgcagacga tgaaaaagat cgtatgaatg
      841 tttccgtagt ttctgcaggc accgcagcag actattcgct tatcggctgc ctgaacttta
      901 cttttttcga catgaaacgt aaagtggtac gcctgaaaca agccggcaga ggcggacttg
      961 gcactgtttt gcgtgataaa aaaatcaaag ccgtggtggc taaggttccc ggagtaaagg
     1021 gcaatctgaa caatgttgtt gacctggaag ctatcatgga acgtggaaaa cgcttcaaca
     1081 aagaaatgcg cgaactcgac gactcgcaat gccagatgcg caaaatcggc actgcccacc
     1141 tcacgcacat catgaacgat tacgacctct tccctaccca taatttcaaa tttggaagcc
     1201 accccgatgc cataaaagtg cacgccgaaa gatataagga acgtttcacg cagaatatgc
     1261 ccgacggatg ctggatagga tgcaatatgt cgtgcgccaa gggtgtggat aattatgaac
     1321 tgaggaccgg cccctacaaa ggatctaaag tgattgttga cggccccgaa tacgaaacag
     1381 cggcctcctt aggatcatgc gccggaatat ttaacccgga atggaccatc gaagccaatt
     1441 tttattgcga cacatacggc atctgcacca tcagctgggg aaccattctt gggttcgtca
     1501 tggaatgcta tgaaaacggt atcatcaatg aagaaagaac cg
//
LOCUS       DAOSFP010000442         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_84337_length_1542_cov_2.814391, whole genome shotgun sequence.
ACCESSION   DAOSFP010000442 DAOSFP010000000
VERSION     DAOSFP010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84337_length_1542_cov_2.814391"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLI16_10385"
     CDS             complement(<1..998)
                     /locus_tag="PLI16_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOH85007.1"
                     /translation="MPIQQPLMTNGQLPNAVNQKPEYFESAVLPYKQTTYLKREMRCA
                     WVATVYCIDYPSTTGLSVNTLKSQYVALISSLKNAGINAIFFQIRPSCDAFYNSPYEP
                     WSEWLMGTQGVAPAGGFDPLVFFVDEAHKQGIEFHAWLNPYRAVVNINSSSVAGTHIS
                     VTHPSWCVTYNTIKLLNPGLPQVRSYLTTVVADIVNRYGVDGIHFDDYFYPYPQTGYV
                     FDDDAAFAADPRGFTNKADWRRDNVNLMVAMVNDTIKSLKPWVKFGIGPFGIWKNGVP
                     AGITGLSSYDAIYCDPIAWLNDHSIDYVSPQLYWKIGTNQDFNTLVDWWGTQAATNG"
     gene            complement(1116..>1542)
                     /locus_tag="PLI16_10390"
     CDS             complement(1116..>1542)
                     /locus_tag="PLI16_10390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH85008.1"
                     /translation="WTNNALNTSVTKNGLSLIDGMTYYFSVKTENGAGLKNICSSDGI
                     TFYIASHIAENSPEQPAYVSPNPFSDNTTLFFELSDEQFIRIILTDMLGKQKILACGN
                     FAAGKHSVGIKKELALAKGNYIVKLEYRDNVKKIKIIKF"
BASE COUNT          408 a          332 c          351 g          451 t
ORIGIN      
        1 ccgttggtgg ccgcctgggt gccccaccag tccactaacg tattgaaatc ctggttggtg
       61 cctattttcc agtataattg cggggagacg taatctatac tatggtcgtt tagccaggca
      121 atagggtcgc aatatatggc atcgtaagat gataagcctg taatccctgc gggaacaccg
      181 tttttccaga taccgaacgg accgattccg aattttaccc agggttttaa tgattttata
      241 gtgtcattta ccatagcaac catcaggttg acattgtctc ttcgccaatc ggccttgttg
      301 gtgaatcccc gcgggtcagc agcaaatgcg gcatcgtcat caaagacata accggtttgg
      361 ggataaggat aaaaataatc atcaaaatgt atcccgtcaa ccccataacg gttgacaata
      421 tcggcgacta ccgtagtcag gtaactgcgc acctgcggca ggccagggtt taacagtttt
      481 atggtgttgt aggtaacaca ccacgaggga tgagttactg atatatgagt gccggcaaca
      541 ctgctactgt taatgttaac cacggccctg tatggattta gccaggcatg aaattcaatg
      601 ccttgtttat gggcttcatc aacaaaaaaa accagcgggt cgaatccccc ggcgggggct
      661 acaccctgcg tgcccataag ccattcggac cacggctcgt aaggactgtt gtaaaaggca
      721 tcacacgaag ggcggatttg aaaaaaaatg gcattaatgc ctgcattttt aagcgaactt
      781 attaatgcga catactggct tttgagcgtg tttaccgaaa gaccggttgt tgaaggatag
      841 tcgatgcaat atacggtggc aacccaggca cagcgcattt ctcttttcag gtaagtggtt
      901 tgtttgtacg gtaaaacagc cgattcaaaa tattcaggct tctgattcac agcattcggc
      961 agttggccat ttgtcatcag tggctgttgt atgggcatgt tgttatccgt ctgttgttgc
     1021 cccatgagtg aaaggcaaca taaaaaagtc agcgccaaaa atgcacatcg gccaaaacat
     1081 ctataaagag tacgtttcat tatccctgtt cctgtttaga atttgataat tttaattttt
     1141 ttgacattgt cgcggtattc taattttaca atgtaatttc cttttgccaa tgccaattct
     1201 tttttaatgc cgacactatg tttaccggca gcgaaattgc cgcatgccag tatcttttgt
     1261 tttcccaaca tgtctgtcag tataatcctg ataaattgtt catcgcttaa ctcaaaaaac
     1321 agggtggtgt tgtcactgaa ggggtttgga gaaacgtatg cgggttgttc cggactgttt
     1381 tcagcgatat gggaagcaat ataaaatgtt ataccatcag aactgcaaat gtttttcaat
     1441 cctgctccgt tttcagtttt tacgctgaag taataggtca ttccgtcgat aagcgacaag
     1501 ccgttttttg taaccgaagt gttcagcgcg ttattcgtcc at
//
LOCUS       DAOSFP010000443         1540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_84485_length_1540_cov_4.602020, whole genome shotgun sequence.
ACCESSION   DAOSFP010000443 DAOSFP010000000
VERSION     DAOSFP010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84485_length_1540_cov_4.602020"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..>1540
                     /locus_tag="PLI16_10395"
     CDS             129..>1540
                     /locus_tag="PLI16_10395"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85009.1"
                     /translation="MLKELLSGYKDDQQVAVLAQLTANDDGAALHINGLNGSAKAFVI
                     AAVFRQSEKTQCVVMPDKESAAYLYNDLENILEEQDVKLFKKNILFFPSSYRHPKNFS
                     KIDNTSVLLRTEVLNKLQKGRKKLVVVTYPDALMEKVASQGFFEKNTLRIKTGDKLSI
                     DFLREIMVEYRFEYVDFVSEPGQYAVRGGIIDVYSFSNENPYRIELMGDEVDSIRTFD
                     PSTQLSLEKIEHIKVVPDSNDSVLVRKKVDFPEYIKGSAAFWFEDAGFAFGQLEMLYP
                     LASEHCRATKDADGIGPDAMEIFSDADSLKKLIPSFPRIEFGKHHFFETRQTIEFTTY
                     PQPSFNKNFELLINQLSENTKNNISNFIFSDSKSQSERISAIIEDIRKSRKQDVDFSV
                     EIINTSIHEGFTDTERKICCFTDHQIFDRYHRFRLKDNYSAREAVTLKEIYNLSPGDY
                     ITHIDHGVGRYAGLEKIEVNG"
BASE COUNT          471 a          321 c          320 g          428 t
ORIGIN      
        1 acaatcattc taaaagttag atgttttatt ctacttttta aagtcttgaa atttgttata
       61 aatacttttt gccacaggaa aattatcttt tgctgtataa agaatacctt tgcaaaaaat
      121 agtttttgtt gctgaaagaa cttctatcgg gttataaaga tgaccagcag gtcgcagtcc
      181 tggcacagct cacggcaaac gacgatgggg ccgccctgca tatcaacggc ctcaacggct
      241 ctgcaaaagc ctttgtcatc gctgccgttt tcaggcaatc tgaaaagaca caatgcgtgg
      301 tgatgcccga caaagagtct gccgcttacc tgtacaacga cctggaaaat atccttgaag
      361 aacaggatgt aaaactattt aagaaaaaca tattgttttt tccttcgtca tacaggcatc
      421 cgaaaaactt ttcaaaaatt gataatactt ctgtactatt gcgtaccgag gtactcaaca
      481 aattacaaaa aggccggaaa aaattagtgg tggttaccta tcctgatgca ctaatggaaa
      541 aagtagcctc acagggtttc tttgaaaaaa acaccctgcg tatcaagact ggcgacaaac
      601 tttctattga ttttctcagg gaaatcatgg tggagtatcg ttttgagtat gttgactttg
      661 tgtcggagcc cggacaatat gcggtcaggg gaggcatcat tgatgtatat tcattttcca
      721 acgaaaaccc ttacagaatt gagttgatgg gtgatgaagt ggattcgata cgcacctttg
      781 acccttccac tcaactctcg ctcgaaaaga tagaacacat caaagttgtc cccgattcta
      841 acgatagtgt cctggtgcga aaaaaggttg actttccaga atatatcaaa ggatcggctg
      901 ctttttggtt cgaagatgcc ggttttgctt tcggacagct ggagatgctg tatccgttag
      961 cttcggaaca ttgccgggca acgaaagatg ccgatggtat aggccccgat gctatggaaa
     1021 tattctccga tgccgacagc ctgaaaaaac tcatcccatc ctttccccgc atagaatttg
     1081 ggaaacatca tttttttgaa actcgccaaa ccattgagtt tactacctat ccgcagcctt
     1141 cttttaataa aaatttcgaa ctgttgataa atcaactttc cgaaaacaca aaaaataaca
     1201 tttcgaattt tatcttttct gattcaaaaa gtcagtcgga acgcataagc gccatcattg
     1261 aggatatccg taaaagccgg aaacaggatg ttgactttag cgtggagata ataaacactt
     1321 caatacacga gggctttacc gacacggagc ggaaaatatg ctgctttacc gaccatcaga
     1381 ttttcgaccg ttatcaccgt ttcaggctga aagataatta cagtgcgcgg gaagcagtaa
     1441 ctttaaaaga gatttataac ctgagtccgg gagattatat tacccacatt gaccacggtg
     1501 tcgggcgtta tgccggcctg gaaaaaattg aagtgaacgg
//
LOCUS       DAOSFP010000444         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_84697_length_1538_cov_3.109238, whole genome shotgun sequence.
ACCESSION   DAOSFP010000444 DAOSFP010000000
VERSION     DAOSFP010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84697_length_1538_cov_3.109238"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLI16_10400"
     CDS             complement(<1..229)
                     /locus_tag="PLI16_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85010.1"
                     /translation="MLGKVIVDKIALLTGSYTGFTYNFDGENHLRALAEAQTGGILVN
                     AHVGNWEFAGQLLERLDTPINILMYDAEHENI"
     gene            complement(473..727)
                     /locus_tag="PLI16_10405"
     CDS             complement(473..727)
                     /locus_tag="PLI16_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009236905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOH85011.1"
                     /translation="MDKQEVINKINLFLVEEFELEPDQLKPEALLKTDLGIDSLDFVD
                     IIVIIEKNFGFKVKGEEMANVRTLQDFYEYVMARVNPTVV"
     gene            complement(742..>1538)
                     /locus_tag="PLI16_10410"
     CDS             complement(742..>1538)
                     /locus_tag="PLI16_10410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOH85012.1"
                     /translation="MNLSVIFKLRGINFTVSAACASGSHAIGLGYFLIKHGLQDMIVC
                     GGAQEVNPFAMGSFDALSAFSVREAEPQKASRPFDKNRDGLVPSGGAATVILESYESA
                     VKRGAPILAEVAGYGFSSNGEHISQSNVEGLIRAIRMSLKDAGMTPRDIEYINAHATS
                     TIHGDIAEAIALADVFADGCPPVSSTKSMTGHECWMAGASEIVYSTLMMQNSFIAPNI
                     NFEEPDEYSEKLNIVPQTLTKDFDIFLSNSFGFGGTNSSLIIKKAR"
BASE COUNT          391 a          324 c          363 g          460 t
ORIGIN      
        1 ttatgttttc gtgttcggcg tcgtacatca gaatgttgat gggtgtgtca aggcgttcga
       61 gcaactggcc ggcaaattcc cagttgccaa cgtgtgcatt gacaagtatg ccgcccgttt
      121 gggcttcggc caatgcacgc aggtggtttt cgccgtcaaa gttgtatgtg aacccggtat
      181 aactacccgt aagaagtgct attttgtcca cgatgacttt acccagcatg caaaaatttt
      241 tataggtgtt cagtacagat cgtattttgc tgtaatgatg aatcttatgg aaataaaaga
      301 atattgcttt ttttgcttta gaggcagaaa acaggaaata tactgctacg aattggagaa
      361 aaaagtaggc aaaagatacc cctaaatgtt ttatgataaa aacaaatatt ttataaccgg
      421 ttacgcctcc gcgggttttc ccttcccatt gcgtcatttg tatcttctga ttttatacta
      481 cggtggggtt gacgcgtgcc atgacgtact cgtagaaatc ctgcagggta cgcacattag
      541 ccatttcttc gccctttacc ttgaaaccaa agtttttttc gataattaca atgatatcta
      601 caaagtccag gctatcaata cccaggtcgg tttttaataa ggcttcaggt ttgagctggt
      661 cgggttccag ttcaaattct tctactaaaa aaagatttat tttgttgatt acttcttgtt
      721 tatccatata aattggtggt tttaccttgc tttttttatg attaatgatg aattggtgcc
      781 gccaaaacca aaagagttcg acaaaaatat atcaaaatct ttagtcagcg tttgtgggac
      841 gatatttagt ttttcagagt attcgtcggg ttcttcaaag ttgatatttg gtgcaataaa
      901 cgagttttgc atcatcagag tggaatatac tatttcgctt gctcctgcca tccagcattc
      961 atggccggtc attgatttgg tggagctgac gggagggcaa ccatcggcaa aaacatcggc
     1021 cagggcgatg gcttcggcga tgtcgccgtg tatggtagag gtagcatgag cgttgatgta
     1081 ttcaatatcg cggggggtca ttccggcatc tttcaacgac atccggatgg cgcgtatcag
     1141 tccttcgaca ttggattgtg aaatatgctc gccgttggat gaaaacccgt aacctgccac
     1201 ttcggcaagt atgggggcgc ctcgttttac ggctgattcg taactttcca gaataactgt
     1261 agcagctccg ccgctgggta ccagcccgtc gcggtttttg tcgaatgggc gtgatgcttt
     1321 ttgcggttct gcttcccgga cagaaaaggc actcaatgcg tcgaaactgc ccatggcaaa
     1381 aggatttact tcctgggcgc cgccgcacac tatcatgtct tgcaatccgt gtttaataag
     1441 aaaatagccg aggccaatgg catgcgatcc gctggcacag gcagcgctaa ccgtgaaatt
     1501 gataccgcgc agtttgaaaa tcaccgaaag gttcatac
//
LOCUS       DAOSFP010000445         1536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_84894_length_1536_cov_2.207292, whole genome shotgun sequence.
ACCESSION   DAOSFP010000445 DAOSFP010000000
VERSION     DAOSFP010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84894_length_1536_cov_2.207292"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PLI16_10415"
     CDS             complement(<1..559)
                     /locus_tag="PLI16_10415"
                     /inference="COORDINATES: protein motif:HMM:NF025757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOH85013.1"
                     /translation="MNTVIPFEHRMSAHCETGTLSSQLAFHGMEISESMVFGIGSGIF
                     FGYFNNSRFAFPTFIVRNQPGKIRENVGSRLGIKFHTQQFKTPEKAQEGLDKILEKGI
                     PAAAQVDFFFMEYLPQYLRVHINVHFINIVGKKDNNYLISDCYHPAIVELDEQALMKG
                     RFAGGSMASNGFLYYPEFIPATFNFE"
     gene            complement(556..1071)
                     /locus_tag="PLI16_10420"
     CDS             complement(556..1071)
                     /locus_tag="PLI16_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85014.1"
                     /translation="MKEQEVNKDYKVKKYILYAEKADGTYGPVEGGSYMMENELDDFW
                     FKKSHIEKLLRERLLKGEISPLYYYMVLQEMTVAELADRAGVCKQKVKCQLKPERFQK
                     ARVGDLLKYAKAFNIPLANFFQIIETSTGLNPNYHFYFEEESKKDAQMVAQHKQDNPL
                     MVVTKIEERKK"
     gene            complement(1096..>1536)
                     /locus_tag="PLI16_10425"
     CDS             complement(1096..>1536)
                     /locus_tag="PLI16_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOH85015.1"
                     /translation="ICLLQLILMIITGMYLLPLIYGMPPLELGSNIFALVCAAFSSAF
                     AAIGFGLLVGTFASTQSQAGIFGSFMVLILAILGGIFLPVYMMPGPMQAASIVSPVRW
                     GVDSFLDIFVREATIDVIGVNLIRLNVFFVVSLLIALLTFVRRK"
BASE COUNT          440 a          356 c          318 g          422 t
ORIGIN      
        1 tttcaaaatt aaaagtagcg ggaataaatt cgggataata taaaaagccg tttgacgcca
       61 tgctgccgcc ggcaaaacgg cctttcatca gggcctgttc atcgagctcc acaatggcgg
      121 gatggtagca gtcgctgatg aggtaattat tgtctttttt cccgacaata ttgataaaat
      181 gcacgttgat atgcacccgc agatactgcg gcaggtattc cataaaaaag aaatcgacct
      241 gggcggcggc agggatacct ttttcaagaa tcttatccag gccttcctga gctttttcag
      301 gagttttgaa ttgctgcgta tgaaatttta tgcccaggcg gctgcccacg ttttccctga
      361 ttttgccggg ttggttacgt acgataaagg ttggaaaagc aaaacgcgaa ttattgaaat
      421 atccgaaaaa tatgccgctg cctatgccaa aaaccatgga ttcggatatt tccataccat
      481 gaaaagccag ttgtgaacta agcgttccgg tttcgcaatg agccgacatc cggtgctcaa
      541 aaggaattac agtattcatt tttttctctc ctcaattttt gtaactacca tcaaaggatt
      601 gtcttgtttg tgctgggcca ccatttgtgc atcctttttt gattcttctt caaaataaaa
      661 atgatagttt gggtttaaac cggttgatgt ttcgatgatt tgaaaaaaat ttgccagggg
      721 tatgttaaat gctttggcat atttgagcag atcacccacg cgggctttct gaaaccgttc
      781 gggtttcagc tggcatttta ccttctgctt acatacaccg gcacggtcgg ccaactcggc
      841 caccgtcatt tcctgcaaca ccatataata gtacaaaggg ctgatttctc cttttaacag
      901 gcgttcccta agcagttttt cgatatggct tttcttaaac cagaaatcat ccagttcgtt
      961 ttccatcata tacgagccgc cttctaccgg gccgtaagtg ccatcggctt tttcggcata
     1021 taaaatgtat ttttttactt tataatcctt gtttacttct tgttctttca taagacataa
     1081 ttataaatta tctgattatt ttctcctgac aaaggtcagc aaggcaatca acaaagatac
     1141 cacaaaaaac acattcaggc ggatgaggtt taccccgata acatcgatgg tagcctctct
     1201 gacaaagata tccagaaaac tatccactcc ccagcgtacc ggcgaaacaa tactggctgc
     1261 ctgcataggc ccgggcatca tgtacaccgg gaggaaaata ccgcccagga tagctaaaat
     1321 cagcaccata aaagagccga atatccctgc ctggctttgt gtggaagcaa aagtccccac
     1381 cagcagcccg aacccgatag cagcaaaagc cgacgaaaag gcagcacata ccaaagcaaa
     1441 aatgttgctt cccagttcaa ggggcggcat accataaatc aaaggcaaaa gatacattcc
     1501 ggtgatgatc atcagtatca actgcagcag gcagat
//
LOCUS       DAOSFP010000446         1534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_85012_length_1534_cov_3.847870, whole genome shotgun sequence.
ACCESSION   DAOSFP010000446 DAOSFP010000000
VERSION     DAOSFP010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85012_length_1534_cov_3.847870"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1534
                     /locus_tag="PLI16_10430"
     CDS             <1..>1534
                     /locus_tag="PLI16_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOH85016.1"
                     /translation="KGLIITDALGMKGASEKVKPGEIFFFKQKTAYDILLMSEDVPLA
                     ITYIQSAIDSGLISQKDIDNKCKKVLRYKYKAGLAKFKPVKINNLYNDLHKTEANILN
                     TKLYQAAVTIIKNNFNILPLKNLENQKIAEISFNADTLNEFAVSAANYAPMAHFCFDN
                     TFDTSAIRTLKEKLFDYNLLLITVNATNNSSLNNYGLTPAIIEFINEIKKSKRVVLNL
                     LLNPYALTKMPDTSGISAIMVAYQSNDITQKATAEAIFGGLAVNGKLPVTAGENFPVN
                     TGFITEQSRLNYVSPVMAGYRADCFYKVDSIARRGIKDAIYPGCQILIAKDGQVLYNK
                     AFGYHTYPTGQAGDTALIPVKTTDLYDVASLTKVLATTISVMKLYEQGKISLDSTLSD
                     YLPGIDSTDKAGILISKLMAHEAQLKAWIPFYEKSMTGNKLDSCVYKSVPSVDYPFRV
                     ADSMYIHKHYFDTIFRAIINSTLRTQNNYLYSDLGFYLLKDIIEKITNEKLDQYVYKN
                     FYR"
BASE COUNT          491 a          338 c          312 g          393 t
ORIGIN      
        1 tcaagggcct tatcattact gatgccttag gcatgaaagg cgccagcgaa aaagtaaaac
       61 ccggtgaaat tttttttttc aagcagaaga cggcatacga tatattgctg atgtcggagg
      121 atgtgccgct cgccataacc tatatccaat cggctatcga cagcggcctg atatcacaaa
      181 aagatattga taacaaatgc aaaaaggtgc ttcggtacaa gtacaaagcc ggtttggcaa
      241 aatttaaacc ggtaaaaatc aacaatcttt ataatgacct gcataagacc gaggcaaaca
      301 ttcttaacac aaaactatat caggctgcag taacgattat caaaaacaat ttcaatatcc
      361 tgccgcttaa aaaccttgaa aaccaaaaga tagcagaaat atcctttaat gccgataccc
      421 tgaatgaatt tgccgtgtcg gcagccaatt acgctcccat ggcgcacttt tgttttgaca
      481 atacttttga taccagtgcc atcaggactt taaaggaaaa actttttgat tacaaccttt
      541 tgctgataac cgtgaatgct accaacaatt cgtcattgaa caattatgga ctgacaccgg
      601 cgattattga gtttatcaat gagataaaaa aaagcaaaag ggtggtactc aatctgctgt
      661 tgaatccata cgctttaacc aaaatgcccg acacgtcggg tatcagcgcc attatggtgg
      721 cttatcaaag caacgacatc actcaaaaag ccacagctga ggcaattttt ggcgggttag
      781 cggttaatgg gaaactccct gttacggcag gcgaaaattt tccggtcaac accggattta
      841 tcacagaaca gagccggctg aattatgttt cgcccgtaat ggcaggatac cgggctgatt
      901 gtttctacaa ggtggattct atcgcccgta ggggtattaa agatgctata taccccggtt
      961 gccagatttt gatagctaaa gatggtcagg tgctttacaa caaggcattt ggataccata
     1021 cctatcccac cggacaagcc ggcgataccg ctttgatccc ggtaaaaacc accgatttgt
     1081 atgatgtggc ttcactcacc aaagttctgg ctaccaccat ttcggtaatg aaactttacg
     1141 aacaggggaa aatatcgctg gactccacac tatcagatta tctccccggc atcgacagta
     1201 ccgataaagc cggcatcctg ataagcaaac tgatggcgca tgaggcccag ctgaaagcgt
     1261 ggataccgtt ttatgaaaaa tcgatgaccg gcaacaaact ggactcgtgt gtgtacaagt
     1321 ctgtgccatc ggtggattat ccattcaggg tagccgacag tatgtacata cataaacatt
     1381 attttgacac catcttcaga gctatcataa attctacact cagaacacaa aataactatc
     1441 tgtacagcga cctgggtttt tacctgctga aagatattat tgaaaaaata accaatgaaa
     1501 agttggatca gtatgtatat aaaaactttt accg
//
LOCUS       DAOSFP010000447         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_86708_length_1515_cov_3.841781, whole genome shotgun sequence.
ACCESSION   DAOSFP010000447 DAOSFP010000000
VERSION     DAOSFP010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86708_length_1515_cov_3.841781"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="PLI16_10435"
     CDS             complement(<1..732)
                     /locus_tag="PLI16_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85017.1"
                     /translation="MENNGYKSRLRSSFISKLFTTIILLTYAVFAIGQVQKPFIQRTS
                     VYSPTKTIYSIKGDFTMIGNTNLTLQNYSDVTLNSNNIMILVDADADATTTNSSSATL
                     TFSTENGAIPSCSNIIYAGLYWTGRTDPYVTTAQKCNIKVKGPGMSYYQTFTANVNDI
                     LYPGDNDMYAGYIEVTDLVQQNGVGEYWVADMALTTGNGSSTGYYGGWGMVVVYENNK
                     MNWRDVTVFDGYAYVVGNTTVSYDLP"
     gene            complement(755..1297)
                     /locus_tag="PLI16_10440"
     CDS             complement(755..1297)
                     /locus_tag="PLI16_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85018.1"
                     /translation="MKKSTFQNQKTIRTKPVAALSLKWFSLIAVPALLLFFSTSIIAQ
                     EAINLETALTSKAPATQQVLKSLVFDVHPTICVENGTIKTFGNGPAVRLETDSKSFSS
                     LYDADPIFDQVEIITVKLNNPDDMKVVPLNLQNLTGFTRLKYILFLCNMNCNPNSINK
                     MFKATTQDGFEIFYQVSIPN"
BASE COUNT          462 a          262 c          314 g          477 t
ORIGIN      
        1 cggcagatcg taacttacag tggtgtttcc cacaacataa gcgtagccgt caaaaacggt
       61 tacgtctctc cagttcattt tgttattttc ataaaccaca accattcccc agccgccgta
      121 atatcctgtt gagctgccat ttccagttgt taaggccatg tcggctaccc agtattctcc
      181 tacaccgttt tgctgcacca gatcggtaac ttcgatatag ccggcataca tgtcattatc
      241 gcccggatac aaaatatcat ttacattggc ggtaaaagtc tgataatatg acataccagg
      301 gcctttgact ttaatattgc atttttgggc agtagtaaca taaggatcag tccttcctgt
      361 ccaatacaaa cctgcgtaaa taatatttga acaggagggt atggcgccgt tttcagtaga
      421 aaaggtcagc gttgccgacg atgagttggt ggtggtggca tcggcatcgg catcaaccag
      481 tatcatgatg ttgttgctgt tgagcgttac atcggaataa ttctgcagtg tgagattggt
      541 gtttccgatc atagtaaaat cgcccttgat actatatatt gttttggttg ggctatatac
      601 tgaagtcctt tgtataaaag gtttctggac ctgccctatg gcgaacacgg cataggtaag
      661 caggataatt gtggtaaaca gtttactgat aaatgaactg cgtaaccttg atttgtaacc
      721 attattttcc attatgtata gattttaatt gttattaatt tggaatggac acctggtaaa
      781 atatttcaaa cccatcttgc gtagtagcct tgaacatttt gttaatagag tttggattgc
      841 agttcatatt gcataaaaag agtatatatt tcaggcgggt gaagccagta aggttttgga
      901 ggttaagggg caccactttc atgtcgtcgg gattatttaa ctttacagta ataatttcaa
      961 cctgatcaaa tattggatct gcgtcataaa ggctggaaaa ggatttagaa tcagtttcta
     1021 atcgaacagc aggaccgtta ccgaacgttt ttattgtgcc gttttcgaca caaatagtgg
     1081 ggtgaacatc aaaaacgaga cttttcagca cttgttgtgt ggccggagct ttgctcgtta
     1141 aggccgtttc gaggttgatc gcctcctgcg ctatgatact ggttgagaaa aacaataaca
     1201 atgcaggaac cgctatcagg gaaaaccatt ttaagcttag agcagcaaca ggttttgtcc
     1261 ggatagtctt ttggttttga aaagtagatt ttttcatcgg gtatttggtt tataggtttg
     1321 aaaatgaatt ttataaaaat tagccaaata tacagcaaaa ttttcgaaaa gtcaactgaa
     1381 aataaaaaaa atagttatca acttttaatt aacattgccc aagtcttgat tttagacgat
     1441 ataattgaaa aaaaataatt taaaaaaatc agaaaagtaa ttttgttgat taattaaaat
     1501 agaataaaga aataa
//
LOCUS       DAOSFP010000448         1501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_87918_length_1501_cov_3.452282, whole genome shotgun sequence.
ACCESSION   DAOSFP010000448 DAOSFP010000000
VERSION     DAOSFP010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87918_length_1501_cov_3.452282"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1344)
                     /locus_tag="PLI16_10445"
     CDS             complement(70..1344)
                     /locus_tag="PLI16_10445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH85019.1"
                     /translation="MKKILLLLCVSFFVSASSAQDTLRVMHYNLMYYNTNTGFCNQEN
                     NSVSRKDSCLKKIIQYIKPDIFTANEIGNNTSSHQRLLDSVLNTGGKNTYKMGQVSTN
                     GGGTLSNGIFYNAEKFTLKSQTNVPTLTTRDINFYEFYFNAVDLGSGTLDTAFLTCIV
                     AHLEPGNTNTTLRAQQIAVLMNYLDSVNVKSNYLVMGDFNVYADTEACYRMLITPGNA
                     DFAFYDPVDKPGLWSDEDDFRFYHTQSTHALQDGCFASGGMDDRFDFILQSEYIQNGT
                     RHYQFLNNSYTTPGQDGNHFNKAINDGANSSAPADIIEALFNMSDHLPVYLDLQVDQT
                     AGLNIQEVFDVAFPNPVTDILHLNVALEKPSQLDFAIINTLSQEVFFYSTTTTGTTAS
                     FEFPVDFLNQGIYFLTMYNGINKPITKKFMKK"
BASE COUNT          417 a          281 c          336 g          467 t
ORIGIN      
        1 aagatggata ctatacagat tgaacaataa atcaggcata atttggattt tttgtacttt
       61 ggcaagacat tattttttca taaatttttt tgttataggt ttgttgatgc cgttgtacat
      121 agtaagaaag tatatcccct gattcagaaa atcaacggga aattcaaaag aagctgttgt
      181 gccggtggtg gtggtgctgt agaaaaaaac ttcctggctg agggtgttta tgatagcaaa
      241 atctaattgg gaaggttttt ccagtgccac gttcagatga aggatgtcgg ttacgggatt
      301 gggaaaagcc acatcgaaaa cttcctgtat attcaatccg gcagtctggt ccacctgcag
      361 gtctaaatat accggtaaat ggtccgacat attgaacaat gcttcaatga tatccgccgg
      421 agcgctgctg ttggcgccat cattaatggc tttgttgaaa tgattgccat cctgccccgg
      481 cgttgtataa gaattgttca ggaactgata gtgccgggtt ccgttttgta tgtattcgct
      541 ttgcagaata aaatcaaagc ggtcgtccat gccgccagat gcaaaacatc catcttgtaa
      601 tgcatgggtg gattgcgtgt gataaaagcg aaaatcatcc tcgtcgctcc acaatcccgg
      661 cttatcaacg gggtcgtaaa aggcaaaatc ggcattgccg ggagtgataa gcatacggta
      721 acaggcttcg gtgtcggcat atacattaaa atcgcccata accaaataat ttgattttac
      781 atttaccgaa tccaggtagt tcattaatac tgctatttgc tgggctctga gggtagtgtt
      841 tgtgttacct ggttccaggt gagccactat acaggtcaga aaagccgtat ccagcgtgcc
      901 tgagcccaga tcaacggcat taaaataaaa ctcataaaag tttatgtccc gtgtggtgag
      961 ggtgggtacg ttggtctggc tttttaatgt gaatttttct gcattataaa atatgccatt
     1021 agacagggtc ccaccgccat tggttgacac ctgtcccatt ttgtaagtgt tttttccgcc
     1081 ggtattcagc acactgtcga gcagacgctg atgtgagctt gtattgttgc ctatttcgtt
     1141 ggcggtgaaa atgtcgggtt ttatatattg aatgatcttt tttaagcaac tatcttttcg
     1201 ggatacagaa ttattttcct ggttgcaaaa ccctgtattg gtattgtaat acatcaggtt
     1261 gtaatgcata acgcgcaaag tgtcctgcgc agaggaggct gaaacaaaaa aagagacaca
     1321 aagtagtaat agaatcttct tcacaaaaaa ataattgcat aaaaatacat tttttgtagc
     1381 gcctggaatt atcttttcta cataaaatta ctaccggccc ttaattattt gagtaaaacc
     1441 tcttgcttga tttgttttgc agcatcttat aacggatttt taccccttgg ggattttccg
     1501 t
//
LOCUS       DAOSFP010000449         1485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_89318_length_1485_cov_3.746154, whole genome shotgun sequence.
ACCESSION   DAOSFP010000449 DAOSFP010000000
VERSION     DAOSFP010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89318_length_1485_cov_3.746154"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PLI16_10450"
     CDS             <1..1482
                     /locus_tag="PLI16_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85020.1"
                     /translation="WILSSCGSRTDIPGNRVFEVKDFKTLTNWNVVDSSNHSLKLIDL
                     DYTNAIDGFTIPSVRQTSDGFFHFKFSIRNLSGQAQKFYYNLYYQNESYKFPECAEGS
                     TKEHPLASENFYGSRSADSLFYAETPLIPGDGKFHEVEFKMKIEGNPRNEKRYFDNTV
                     NQRWKRNPRAGEYSFLLVVSTKDNIDNMIIPEYIADVSKQRDSVFHNPYYYLLFGEGK
                     NKNNMEVLHSGNVLKIVARPGLDDGIYVNPADFIAEKYLPSTHKYCGSSDELYQNAPL
                     RTFVSYVDTSSRFYNIPVLADVINENYSLRDYNWNRAFHKQEELVSTKPGNTDCPCSQ
                     LAVDTVEASVTMKNEATRPGEWKKKSIGVMTRHGLTYGKYTVKVKMTELLNKAGVWNG
                     ITNGIWLASQKNEPWNNCRKCNKKGYLHNYWGSGKDERSDYTGYSEIDFEILKTVSYC
                     PSYQFPPTYYYPVANRYCVADWHVPLPRALQPDSANINIGTDD"
BASE COUNT          446 a          302 c          340 g          397 t
ORIGIN      
        1 tggatattgt cgtcgtgcgg aagccgtact gatattcctg gaaaccgggt ttttgaagta
       61 aaagatttta aaacactgac aaactggaat gtggttgaca gcagtaacca ttccttgaag
      121 cttattgatc ttgactatac caacgccatc gacggtttta caatcccttc tgtgcgccag
      181 acatcagacg gtttttttca ttttaaattt tctatcagga atctttccgg ccaggcacag
      241 aagttttatt ataatcttta ctaccagaac gaaagttata agtttccgga atgtgccgaa
      301 ggtagtacaa aggagcatcc tctggccagc gaaaattttt atggaagccg aagtgctgat
      361 tcgctctttt atgccgaaac accgcttatt cctggcgatg gaaagtttca tgaggttgaa
      421 ttcaaaatga agattgaagg gaatccgcgc aatgaaaaaa gatatttcga taatactgtt
      481 aatcaacgct ggaaacgaaa tcccagggca ggggagtata gttttttgct ggtagttagt
      541 accaaagaca atatcgataa tatgataatt ccggaataca ttgccgatgt gagcaagcag
      601 cgcgacagtg tctttcataa tccgtattat tatcttttat tcggagaagg aaaaaataaa
      661 aataatatgg aggtgttgca ttcggggaat gtccttaaaa ttgttgcccg gcccgggctg
      721 gatgacggaa tttatgtcaa tccggcggat ttcatcgctg aaaaatattt gccatccaca
      781 cacaaatatt gcggtagtag tgatgaatta taccaaaatg ctccgctgag gacttttgta
      841 agctatgtgg acacatcttc aagattttat aacattccgg tgcttgcaga tgtgatcaat
      901 gaaaattatt ctttgcgcga ctataactgg aaccgcgctt ttcataaaca ggaagaactg
      961 gtgtcaacaa aaccgggcaa taccgattgt ccatgcagcc agcttgccgt tgatacggtg
     1021 gaggcctcgg ttaccatgaa aaacgaagcc acacgccccg gcgaatggaa aaagaagagt
     1081 atcggcgtaa tgacccggca cggcctgaca tacggaaaat atacggtgaa agtaaaaatg
     1141 accgaattgc tcaacaaggc tggtgtgtgg aacggtatca ccaatgggat ttggctggca
     1201 tcgcagaaaa acgagccatg gaacaattgc cgcaaatgca ataaaaaagg gtacctgcat
     1261 aactattggg gttccggaaa agatgaaagg tcggactata ccggctattc agagatagat
     1321 tttgaaatcc tgaaaaccgt aagttattgc ccttcctatc aatttcctcc cacttattat
     1381 tatccggtgg ctaaccgtta ctgcgtagcc gactggcatg ttcccctgcc tcgggctctg
     1441 cagcccgatt ccgccaacat taatatcggg accgatgatt gaatt
//
LOCUS       DAOSFP010000450         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_89423_length_1484_cov_3.149055, whole genome shotgun sequence.
ACCESSION   DAOSFP010000450 DAOSFP010000000
VERSION     DAOSFP010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89423_length_1484_cov_3.149055"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..510
                     /locus_tag="PLI16_10455"
     CDS             85..510
                     /locus_tag="PLI16_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HOH85021.1"
                     /translation="MIAQGSEILKLIPQRPPIVMVDKLISAAGKKTVSGLKITLDNIF
                     VADNCLQEPGIIENIAQSAALGVGYLCQAKNEKIPIGFIGAVSNLKIFSLPRVNTEII
                     TEIQVEYEVLEATIISSKVFCDKQLLAQAEMKIFLKTEN"
     gene            534..968
                     /locus_tag="PLI16_10460"
     CDS             534..968
                     /locus_tag="PLI16_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85022.1"
                     /translation="MGKEKKDNNKSHIVDLENLDVDHRKFKITHYQMEKNGHFSHDMK
                     SDWGGRDPVVNQSLVIIKERINIARQQVINGEVSPIAYYMEKCFFDLKTLAQYIGIAK
                     WRVKRHLKVKIFKKLKDETLKRYADFFGISIDELKDIDHLYH"
     gene            984..>1484
                     /locus_tag="PLI16_10465"
     CDS             984..>1484
                     /locus_tag="PLI16_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HOH85023.1"
                     /translation="MKMNFDHMQLSAHCESGATVNMLGYYNIKITEPMVFGIGAGYYF
                     GHLPFLKMNYSPATTFRALPGLVFSRTCKNLGISYVRNTYRDPKKAMDELDRVLDQGI
                     PVGVQVGVFQLPFFPPEYRMHYNMHNMVVYGKENNNYFVADSVVDGENIISYDDLMRV
                     RYAKGAF"
BASE COUNT          496 a          335 c          299 g          354 t
ORIGIN      
        1 gaagacaccg cggcaattac caacaaaatg agaacactgc gaaacaaaaa aggccccctg
       61 tgggagtatc taatccaata atttatgatt gcacaaggta gtgaaattct taaacttatc
      121 cctcaaaggc cgcccattgt catggtggat aaactgattt cggctgccgg caaaaaaacc
      181 gtcagcggcc tgaaaatcac actggataat atttttgtag ccgacaactg cctgcaggaa
      241 cccggtatca ttgaaaacat agcacagtca gctgccctgg gtgtaggata cctttgccag
      301 gcaaaaaacg aaaaaattcc tataggattt atcggcgccg tgagcaacct caagattttc
      361 agcctcccca gggtaaacac cgagataatt accgaaatac aggtagaata cgaagttttg
      421 gaagccacca tcatcagcag caaagtattt tgcgataagc agctcctggc ccaggccgaa
      481 atgaaaattt ttctcaaaac cgaaaattaa aagcataatt acacgaaatc tttatgggca
      541 aagaaaaaaa agacaacaac aaaagtcata tcgtagacct cgaaaacctg gacgttgacc
      601 atagaaaatt taaaatcacg cattaccaga tggaaaaaaa cggccatttt tcacacgaca
      661 tgaaatccga ttggggtggc cgcgatcccg tggttaacca aagtctcgta ataattaaag
      721 aaaggataaa catagccagg caacaggtga ttaacggcga agttagcccc attgcctatt
      781 atatggaaaa atgctttttc gatctgaaaa cccttgccca atatattggc atagccaaat
      841 ggcgggttaa aagacacctg aaagtaaaaa tattcaaaaa actgaaagac gaaacactga
      901 aacgctatgc cgactttttt ggcatttcca tagatgaatt aaaagatatt gaccacttat
      961 atcattaaat accaacacgt tatatgaaga tgaatttcga ccacatgcaa ctatctgccc
     1021 attgcgaaag tggcgctacc gtcaatatgc tgggatacta taacataaaa attaccgaac
     1081 ccatggtttt tggcattggc gccgggtatt attttggcca tctgcccttt ctaaaaatga
     1141 actattcccc cgccaccacc ttcagagctt tgccaggcct tgttttcagc cgtacctgca
     1201 aaaacctggg aatatcatat gtgcgcaaca cctaccgcga cccgaaaaaa gccatggacg
     1261 agctcgaccg ggtactcgac caaggcatcc ctgtgggcgt gcaggtgggt gttttccagt
     1321 tacctttttt tccacctgaa taccgcatgc attacaatat gcacaacatg gttgtgtatg
     1381 gaaaagaaaa taacaactat tttgtggccg atagtgtggt agatggcgaa aatataattt
     1441 catacgacga cttgatgcgc gtacgttatg ccaaaggcgc cttt
//
LOCUS       DAOSFP010000451         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_89685_length_1481_cov_3.509818, whole genome shotgun sequence.
ACCESSION   DAOSFP010000451 DAOSFP010000000
VERSION     DAOSFP010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89685_length_1481_cov_3.509818"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLI16_10470"
     CDS             complement(<1..599)
                     /locus_tag="PLI16_10470"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOH85024.1"
                     /translation="MHFNIPIFIPHLGCPFQCVFCNQKYISGVQNAPDIEEVHAIIRQ
                     KLSTIGGQGARVELAFFGGSFTCLSLEEQRYYLEAIQPYIAENKINGIRISTRPDFID
                     DERLQMLKKFQVQTIELGVQSLNDSTLKKICRGHNVADVRNAAKMIKSYGMALGIQTM
                     VGLPGEDSAMELETAREVIALQPDCVRIYPLLVLRDTKL"
     gene            complement(614..913)
                     /locus_tag="PLI16_10475"
     CDS             complement(614..913)
                     /locus_tag="PLI16_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOH85025.1"
                     /translation="METNKILSACGLWCDECEHYGKLCDGCHAVQGSTFWAKEMMPSK
                     VCPLYDCGVNQRGYRDCGQCAELPCATFLQMKDPNSTEEEHQQMLKIRQERLRSS"
     gene            complement(1020..1463)
                     /locus_tag="PLI16_10480"
     CDS             complement(1020..1463)
                     /locus_tag="PLI16_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85026.1"
                     /translation="MNNKNYDYSLTPGSMPEVEHNEDFIRFGKVCYLGFEEFFHLIIL
                     INFEVTYSTDGKRIILFSPHRMQAGFITKNNIMNKKILTLNNQFEAIRIKEILDVNEI
                     PHMIRSFHDSAYDGVFQAQYGWGALEADEKDEAKILELIKEVFDK"
BASE COUNT          390 a          326 c          298 g          467 t
ORIGIN      
        1 tcgagtttag tatcccgaag taccagtaaa gggtatatcc tcacacagtc gggctgcaag
       61 gctatcactt ccctggcggt ttccagttcc atggcgctat cctctcccgg aaggccaacc
      121 atagtttgaa ttcccagtgc catgccgtat gattttatca ttttggctgc gttccgcaca
      181 tcggcaacat tatgtcccct gcagattttt ttcagggtgc tgtcgttcaa cgactgtacg
      241 ccaagttcga tagtttgcac ctggaatttt ttcagcatct gtaatctttc gtcatcaata
      301 aaatcgggcc gtgtggatat gcggatgcca tttattttat tttcagcaat atagggctgt
      361 atggcttcca ggtaatagcg ttgttcctca agactcaggc aggtaaagct accgccaaaa
      421 aaagccagct ctaccctggc gccctgcccg ccgatggtgg aaagtttttg ccggatgatg
      481 gcgtggactt cttcaatatc gggtgcgttt tgtactccgg aaatatattt ctggttacaa
      541 aaaacacatt gaaacgggca gcccagatgt ggaataaata tggggatgtt aaaatgcacc
      601 ggacaatggt cttttaactt gacctcaggc gttcctgcct tattttcagc atttgctgat
      661 gttcttcttc agtagaattc gggtctttca tctgcaaaaa tgttgcacac ggaagttcgg
      721 cacattggcc gcaatcgcgg tagccacgct ggtttacccc gcagtcgtac aaagggcaca
      781 ctttggaagg catcatttct tttgcccaga atgtggaacc ttgaacagca tggcacccat
      841 cgcacaattt cccgtagtgt tcacattcat cgcaccacaa tccgcaggct gaaagtattt
      901 tattagtttc catagaatag ttttttacaa aaatacgaca ggttttcttt aaaacaaata
      961 aaccgaatga ttttaatatt acctgctaac aagtgttgat gcgccaggca gtccctgctt
     1021 tatttatcaa atacttcttt tattaattct aaaatttttg cttcatcttt ttcatcggct
     1081 tccagtgcac cccaaccgta ttgggcctgg aatacgccat cataggcgga atcatgaaaa
     1141 cttctgatca tatgaggaat ttcattgacg tcaagaattt cttttatcct tatcgcttca
     1201 aattggttgt taagtgttaa gatttttttg ttcataatgt tgtttttagt tataaaaccg
     1261 gcttgcatcc gatggggtga aaacaaaatt atacgtttgc catctgttga atacgtgact
     1321 tcaaagttaa tcaaaattat caaatgaaaa aattcctcaa agcctaaata gcagaccttg
     1381 ccgaaccgta tgaaatcttc attatgctca acctccggca tgcttccagg ggtgagagaa
     1441 taatcataat ttttattgtt catttgtcag gcttatgtat a
//
LOCUS       DAOSFP010000452         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_90400_length_1473_cov_4.626234, whole genome shotgun sequence.
ACCESSION   DAOSFP010000452 DAOSFP010000000
VERSION     DAOSFP010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90400_length_1473_cov_4.626234"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLI16_10485"
     CDS             complement(<1..391)
                     /locus_tag="PLI16_10485"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOH85027.1"
                     /translation="MRQVFKYLVVAAIIFFCVIVFSKITADQVVDLPVPTNDSTLTIL
                     FAGDVMQHEPQILSAWDDSLQAYSYDSCFSFVAPLIQDCDIAMANFEFTLGGPPYTGY
                     PQFSAPDQIVNALINAGFDIMATANNHS"
     gene            complement(408..983)
                     /locus_tag="PLI16_10490"
     CDS             complement(408..983)
                     /locus_tag="PLI16_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85028.1"
                     /translation="MQRNFMVSIVLLGCIILFSSYRIIPVKTVSPDKSDSTFLITDGK
                     VGKVEINMDVNKLKNIFPESQMKEKKVDSEGDEYALYMILEPDNKTLAMEIESLCADI
                     CLVSRINILLPKYKTMEGVGVGSPFAEIKKNYTMKNIVGGEDNSFMVYVKEYRDIAFI
                     VKSAEKKPKFGKPYEASEILGTDVVKQIFIF"
     gene            complement(1134..>1473)
                     /locus_tag="PLI16_10495"
     CDS             complement(1134..>1473)
                     /locus_tag="PLI16_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85029.1"
                     /translation="WVFHNEMLSLFTADPKLITESLPSYYVVMATLIIFSLTMNYYNG
                     VIGTGSTRVSMLLEVIGVVFYLIFVYFVVLVLNVSLEFAWFSEAEYFISMCILSLIYL
                     KKGSWKRKII"
BASE COUNT          464 a          311 c          283 g          415 t
ORIGIN      
        1 aggagtgatt attggccgtt gccataatat cgaaacctgc attgataagc gcgtttacta
       61 tctgatctgg agcgctgaac tgcggatagc ccgtataagg aggacctcca agggtaaact
      121 cgaaatttgc catggcaata tcacagtctt gtatcagcgg cgcaacaaaa ctaaagcagg
      181 aatcatagct atacgcttgt aatgaatcgt cccaggcaga gagtatctga ggttcgtgtt
      241 gcatcacatc gccggcaaac agtatggtca gtgtagaatc attggtgggc accggcaggt
      301 caacaacctg gtcggccgta attttcgaaa aaacgattac acaaaagaat ataatcgcag
      361 caacaacaag gtatttaaat acttgtctca caattgaaaa gctctgatca gaaaataaaa
      421 atctgcttca ccacatcagt gccaagtatc tccgaagcct cgtaaggttt gccgaatttg
      481 ggcttttttt cggccgactt cacaataaag gctatatccc tgtattcttt aacataaacc
      541 ataaaactat tgtcttcgcc acctacaata tttttcatgg tataattctt ttttatttcg
      601 gcaaaaggac ttccaacgcc aacgccttcc atagttttgt atttgggtaa caaaatgttt
      661 atacgcgaca caaggcagat atccgcgcaa agtgattcta tttccatggc aagggttttg
      721 ttatcgggtt ccagtatcat gtacaaagca tattcatcac cttcggaatc aacttttttt
      781 tccttcattt gactttcggg aaaaatattc ttcagcttgt ttacgtccat gttaatctcc
      841 actttaccta ctttgccgtc ggtaatcagg aacgttgaat cgcttttatc cggcgagaca
      901 gtttttacag gaattattct atacgatgag aacaagatga tacagcctaa aagcactatg
      961 gataccatga aatttctttg cataccgttt tatttttgag ttgataaaag tacaaaaaga
     1021 attgcttgca gctaaatagt gtctataaaa aactcgtgtt tgtaaaaaga aaacgtcaag
     1081 tttgaggact gcccgacgca ggcggggaaa aaattggcgt tttcttacaa acactaaata
     1141 atctttcgtt tccagctccc ttttttgaga tatatcaagg aaaggataca catcgaaata
     1201 aaatattcgg cctctgaaaa ccatgcaaat tccagggata cattcaaaac cagcaccacg
     1261 aaatagacaa aaatcagata aaaaaccacc ccgatgactt caagcaacat gctcacacgt
     1321 gtactgccgg tgcctatcac gccgttgtaa taattcatgg taagcgaaaa tatgattagt
     1381 gtggccatca ccacatagta ggacggcagg ctttcggtga ttaatttggg gtctgcagta
     1441 aacaacgaca acatttcatt gtgaaacacc cat
//
LOCUS       DAOSFP010000453         1473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_90452_length_1473_cov_2.516925, whole genome shotgun sequence.
ACCESSION   DAOSFP010000453 DAOSFP010000000
VERSION     DAOSFP010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90452_length_1473_cov_2.516925"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLI16_10500"
     CDS             complement(<1..143)
                     /locus_tag="PLI16_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOH85030.1"
                     /translation="MTVNFYKYHGAGNDFVVIDNRLNIFFPAPEVVNRLCHRRFGIGA
                     DGML"
     gene            complement(140..859)
                     /locus_tag="PLI16_10505"
     CDS             complement(140..859)
                     /locus_tag="PLI16_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85031.1"
                     /translation="MSLKVNKIIYMLLGIVLGFFVGGGIIWWQVNDKDFTDFFRKTND
                     KPSLDTNQYDRSIRPDKKSSHNFRVGNTEIPFTYIDSLKKDSLNPSLDELLAMYANAS
                     SDSNVRSSGSSYDDIVVSKDELIITRLIKISGEVPEIDNGYELDSLLTDQRQTKRKPK
                     GIIKTEFWKSPLNYKGYKFDENKLVIFGLYEVNNVSITGYNNELFVKYNDIYYLIEYT
                     DDFKSFFPMKNEMLLKELNSL"
     gene            complement(1090..>1473)
                     /locus_tag="PLI16_10510"
     CDS             complement(1090..>1473)
                     /locus_tag="PLI16_10510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOH85032.1"
                     /translation="EVNYEQITGTPGQSARYFFDDFVLCKCEDTITPAGPEQEVFIPN
                     IFSPNGDRQNDVFMVRGENITELNLSVYNRWGNKVFEGSGPQTAWDGTYNGKTCAGGV
                     FYYMAEIGFVGGKREMRKGNVTLVR"
BASE COUNT          414 a          319 c          286 g          454 t
ORIGIN      
        1 agcataccgt cggcgccaat gccgaaacgc cggtgacaga gccggttgac tacttccgga
       61 gccgggaaaa atatgttcag cctgttatca atgacgacaa aatcgttgcc tgctccatga
      121 tacttataaa aattaacggt cataacgagt tcagttcttt aagaagcatt tcatttttca
      181 taggaaaaaa tgatttgaaa tcatcggtat attctatcag ataatagata tcgttatatt
      241 tgacaaaaag ctcgttgttg tagccggtga tagaaacatt gttcacttcg tagaggccaa
      301 aaatcacgag tttattttca tcaaatttgt atcctttgta attgagtggc gatttccaga
      361 attccgtttt tattatgcct ttgggttttc gttttgtctg gcgctggtcg gtgagcaggg
      421 agtcaagttc gtagccgtta tcaatttcgg ggacttcgcc ggaaattttt atcagtcggg
      481 taatgataag ttcgtctttg gaaaccacga tatcgtcgta cgaagagcct gaggaacgta
      541 cgttgctgtc ggaacttgca ttggcataca tagcaaggag ttcgtcgaga gagggattga
      601 ggctgtcttt tttcaggctg tcgatatagg taaaaggaat ttcggtattt cccacgcgga
      661 aattatgaga agatttttta tccggcctta tagaacggtc gtattggttg gtatcaaggg
      721 agggcttgtc gtttgttttc ctgaaaaaat ctgtaaagtc tttgtcgttt acctgccacc
      781 agatgatgcc gccgccaaca aaaaaaccga gcacgatgcc cagcagcata tagattattt
      841 tgttgacttt taaactcatg gtatgatttt agcttaggct gcaaattcac aacaaaagta
      901 atactatttc agggataaag ggatattaac aatcagccta gtgtaatatt tttttataaa
      961 aaaatccgat aaatcctcag accaatggga acaaaagaca actaaatgtt aacttaatta
     1021 tgttacaacc tgtcaacgct atatacagcc acatacaatt acttttaatt cctaaatttt
     1081 ccaccttccc tacctcacca gcgtcacatt ccccttcctc atctcccgtt tgcctcccac
     1141 aaatccaatt tccgccatat aataaaacac gccaccggca cacgtcttgc cgttgtatgt
     1201 gccatcccat gctgtctgtg gcccactgcc ctcaaatacc ttgttacccc agcggttata
     1261 aacacttaaa tttaattctg ttatattttc gccccgcacc atgaacacat cattttgcct
     1321 atcgccgttt ggtgaaaaaa tattgggtat aaagacctct tgctccggcc ctgctggcgt
     1381 tatcgtatct tcgcatttgc ataaaacaaa atcatcaaag aaataccggg cactttgacc
     1441 tggtgtacct gttatctgtt cataatttac ttc
//
LOCUS       DAOSFP010000454         1377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_100323_length_1377_cov_3.521180, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000454 DAOSFP010000000
VERSION     DAOSFP010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100323_length_1377_cov_3.521180"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..>1377)
                     /locus_tag="PLI16_10515"
     CDS             complement(130..>1377)
                     /locus_tag="PLI16_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85033.1"
                     /translation="YVLNYFSRREAVGIERTREDYYRLMTDQAIRTIYPMAAYKDSSG
                     NFTLNLDVKKESNILLRFGGLVSSNPVNQAFAEVSYKYLQRNAATLRANMYAGRFYSS
                     GLLGMRLDFPGHPQYALFGRIVYNQWDYFRTTSHFFEDKNPSYLIINDYHWDCGVVVP
                     VRNKGKFTLAGSAMIIDDEYFQDNYFTRTDIPDKTNFKAWSASAEWERNTLNKKQYAN
                     KGTLLYLKGGILKGKENTVPGTTSLLSGTYTANHDWLYLQFICENYFRSVGRFRLGWY
                     SDLYFSDRDFFGSYTATMLFANPFQPLPEMKTLFMPQYRAHQYAGAGLKSIFMIRDNF
                     DFRAEAYLCQPLRDITMGPSGIYATYGPWLRSRYFVASAGLVFHSPVGPVAITASYYD
                     KYDEPFMLSFYFGYILFNPKAVN"
BASE COUNT          401 a          325 c          269 g          382 t
ORIGIN      
        1 acttcgcttt cattcagctt agaatcagca ttttcagccg gggtttcttt cttttcttcc
       61 atcactttga aattcaatat tcacaataag gactgcaaat ttaaaaaact taattaatta
      121 gaggcagttt cagttaactg ctttcggatt aaataagata tatccgaaat aaaacgatag
      181 catgaatggt tcatcatact tgtcataata gctggcagta atggcaaccg gtcccaccgg
      241 gctgtgaaag accaatcctg ccgacgccac aaaatacctg cttctgagcc agggaccata
      301 ggtcgcataa atgccgctcg ggcccatggt aatatcacgc aagggttggc acaaataggc
      361 ctctgcacgg aaatcaaaat tatccctgat catgaaaatg ctttttagcc ccgcacctgc
      421 atattgatgt gccctgtact gcggcataaa caatgttttc atttccggta atggctgaaa
      481 aggattggca aacagcattg ttgccgtata actcccgaaa aaatcccggt ccgagaaata
      541 taagtcgctg taccagccaa gccggaaccg gcccacggaa cggaaatagt tttcacatat
      601 gaactgcagg tacagccagt catgattggc cgtgtacgtt cccgacagta aagaagttgt
      661 tcccggcacc gtgttttctt ttccttttaa gatacctccc tttaaataca gcaatgtgcc
      721 tttgtttgcg tattgcttct tgttgagcgt gtttctctcc cactccgcag atgcgctcca
      781 ggccttgaag tttgttttat caggaatatc cgttctggta aaataattgt cctgaaaata
      841 ctcatcgtca ataatcattg ccgaaccggc taaagtgaat tttcctttgt ttcttacagg
      901 tacgacaaca ccgcaatccc agtgataatc attgattatg agatagctcg gatttttatc
      961 ctcgaaaaaa tgtgatgttg ttctgaaata atcccactga ttataaacaa tccgcccaaa
     1021 cagcgcatat tgcggatggc cgggaaaatc gagccgcata cccagcaaac cagaactgta
     1081 aaaacgccct gcatacatgt ttgcacgtaa agtagccgca tttcgctgaa ggtatttgta
     1141 actcacttcg gcaaaggcct gattgaccgg attggatgaa accagaccac caaagcgcag
     1201 cagaatattc gactcctttt ttacatcaag attcagtgta aaatttccgc ttgaatcctt
     1261 atatgcagcc atcggataaa tagtacgaat ggcctggtct gtcatcaaac gataataatc
     1321 ttcgcgcgtc ctttcaatac caactgcttc gcgacgcgaa aaataattca gtacata
//
LOCUS       DAOSFP010000455         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_100673_length_1374_cov_3.308567, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000455 DAOSFP010000000
VERSION     DAOSFP010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100673_length_1374_cov_3.308567"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..899)
                     /locus_tag="PLI16_10520"
     CDS             complement(81..899)
                     /locus_tag="PLI16_10520"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOH85034.1"
                     /translation="MVADLKLMVDAAWDNRALLKEDNTCSAIRRVVDMLDKGTLRVCE
                     PGDTGWILNEWVKKAVILYFPIQQMETIEVGPFEFRDKIKLKKNYESLGVRVVPHAIA
                     RYGAFLAPGVVMMPSYVNIGAYVDSGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLE
                     PVQAAPVIIEDNCFIGSRCIVVEGVHVKAEAVLGANVVITKSTKIIDVSGNEPVEFHG
                     VIPERSVVIPGTYLKEFPAGKYPVTCALIIGKRKESTNTKTSLNECLRDFNVAV"
     gene            1017..>1374
                     /gene="ruvX"
                     /locus_tag="PLI16_10525"
     CDS             1017..>1374
                     /gene="ruvX"
                     /locus_tag="PLI16_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOH85035.1"
                     /translation="MGRLLAIDYGQKRTGIAVTDELQLIANGLTTVNSSELIPFLKNY
                     CSKEKVELIVVGQPLQMNNTASESEKFIIPFLKALQKSLPGIPVKRYDERFTSKMAFQ
                     TMIESGISKKKRQDKAL"
BASE COUNT          416 a          330 c          253 g          375 t
ORIGIN      
        1 cccgcgggaa gctgttggtg caaacttttt atcttatcag gaattgtcat ataatctgta
       61 aatacaaatt ttcataaaaa tcaaacagcc acattaaaat ctctcaggca ttcattcagc
      121 gaggttttcg tgttcgtaga ttctttgcgt tttccaatga tcagggcaca ggtaaccggg
      181 tattttccgg ccggaaattc tttcaggtat gttcctggta ttaccaccga cctttcaggt
      241 attacaccat ggaattcgac aggttcattt ccactgacat caataatctt tgtagatttt
      301 gttatcacta cgttagcgcc cagcacggct tcggctttaa catggactcc ttcgaccaca
      361 atgcaccgtg agccgataaa acaattatct tcaataatca ctggcgcagc ctgcacaggc
      421 tccaaaacgc cacctattcc cacgccgccg ctcaggtgta catttttgcc aatctgcgca
      481 caggagccca cggtagccca ggtatccacc atcgttccgc tatcaacata agcgccaata
      541 ttaacataag acggcatcat caccacacca ggcgctaaaa aagcgccata gcgggctata
      601 gcgtgcggaa caacgcgcac ccccaaagat tcgtaattct ttttcagttt tattttatcg
      661 cgaaattcaa aaggtcccac ctcgatggtt tccatctgtt gaattggaaa atacaaaata
      721 acggcctttt ttacccattc attcaaaatc catcccgtat cgccgggttc acacacacgc
      781 agcgtaccct tgtcgagcat atcaacaacc ctgcgaatgg cactgcaggt gttgtcttct
      841 ttgagcaagg cacgattgtc ccatgcagca tcaaccataa gttttaaatc agcaaccata
      901 aaatttgttt ttgcagtaag cgttcaaagt taaaaaaaaa tgtgaaacag ccattttcac
      961 acgtaaaata tagtaggatt aaggcatttt tcgtaccttt gcaccacgaa aaaaatatgg
     1021 gacgtttact tgctatcgac tacggacaaa aaaggacagg cattgcagtt actgatgaac
     1081 ttcagttgat tgccaatgga ttgacaaccg ttaattccag cgagctgata ccttttctga
     1141 aaaactactg ttccaaagaa aaagttgaac ttattgtagt cggccaacct ttgcagatga
     1201 acaatacagc ttcggaatca gaaaaattta tcattccgtt tttgaaagca ctgcaaaaat
     1261 ccctacctgg tatccctgta aaaagatatg acgaacgttt tacttccaaa atggcttttc
     1321 aaactatgat tgaatcgggt atcagcaaaa agaaacgtca ggataaagcc ctgg
//
LOCUS       DAOSFP010000456         1368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_101342_length_1368_cov_3.082254, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000456 DAOSFP010000000
VERSION     DAOSFP010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101342_length_1368_cov_3.082254"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PLI16_10530"
     CDS             complement(<1..382)
                     /locus_tag="PLI16_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOH85036.1"
                     /translation="MFESCSYLFLQQYWWIIVSLLAAFFIFLTFVQGGQTLLGALSAS
                     EKEKNMLVNAIGRKWEFTFTTLVTFGGAMFAAFPKFYSTSFGGAYWVWIAILFCFVVQ
                     AVSYEFRKKPGNVFGAKTYEIFLYI"
     gene            complement(375..>1368)
                     /locus_tag="PLI16_10535"
     CDS             complement(375..>1368)
                     /locus_tag="PLI16_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOH85037.1"
                     /translation="VAVNKFLHTISSSYVVASLFIVSVSSWYLLKKRELLLARRSIVL
                     ASVFGLLSALFTAITGDGSAYMVAQKQPAKLAAMEGLYQGKSGAGLIVFGVLKADAHK
                     DKEDNFHVKFEVPKVLSLLGYRSPEAFVPGIKDLTDGNKEHNIMPTSEKMEKGRLALA
                     SLCSFKEYKKQNNQAKVDSSLGIFRANAQYMGYGYLNDPNSIIPNVPMTFYSFHIMVG
                     LGTLFILLFMITLYLSLKNKLDKQRLLLKLLILFLPLGYVASESGWIVAEVGRQPWAI
                     QDLMPVSVATTRIDATAVMTTFILFAVLFTVLLIAEIKIMLTQIKTGPEGGKNV"
BASE COUNT          385 a          318 c          306 g          359 t
ORIGIN      
        1 tgatgtataa aaatatttcg taagttttag ccccgaacac attgccgggc ttttttctga
       61 attcgtaaga aactgcttgt accacaaaac agaacaatat ggctatccaa acccagtatg
      121 cgccgccgaa acttgtggaa taaaacttgg ggaaagcggc aaacatagct cctccaaacg
      181 tgaccagggt ggtaaaggta aattcccatt tacggccaat ggcattaacc agcatgtttt
      241 tttctttttc actggcagat aacgccccca ggagggtttg tccgccctgc acaaacgtga
      301 gaaaaatgaa aaaagccgcc agcagtgaaa ctattatcca ccaatattgc tgcaggaaca
      361 gataagaaca actttcaaac attttttcct ccttccgggc ctgtttttat ttgtgtcagc
      421 ataattttta tttcagcaat aagcaatacg gtaaataaca ctgcgaaaag gataaaagtt
      481 gtcatgacag cggttgcatc aatccgcgtg gtggccaccg aaacaggcat caggtcctgt
      541 attgcccagg gctgacggcc cacttcggca actatccagc cgctttcgga agccacataa
      601 cccagtggca ggaataatat cagcaatttc agcagcagcc tttgtttgtc gagtttgttt
      661 ttcaacgaca ggtaaagggt aatcataaac agcaaaatga ataatgtccc caacccaacc
      721 atgatgtgga aactgtaaaa tgtcattggt acattgggaa taatgctgtt ggggtcgtta
      781 agatatccgt accccatata ttgtgcattg gccctgaaaa tgcccaggga ggaatcaact
      841 ttggcttggt tattctgttt tttgtactct ttaaatgagc agagtgatgc taaggcaagc
      901 cgtccctttt ccattttttc ggaggtcggc atgatattgt gttctttgtt cccatcggtc
      961 aggtctttga tgcccggcac aaaggcttcg gggctgcggt aaccaagcag cgataatact
     1021 ttgggaactt caaacttaac gtgaaaattg tcttccttgt ctttgtgcgc atcggcttta
     1081 agcactccga aaacaataag gcctgccccc gatttgccct gataaagccc ttccatggcg
     1141 gctaatttgg ccggttgttt ttgtgctacc atataagccg aaccatcgcc ggtgatggcg
     1201 gtaaacagag cggataacaa cccgaatacc gaggcaagca ctatgctacg ccgggccagt
     1261 aacagctcgc gttttttcag caggtaccat gaactgacac tcacaatgaa taaggacgct
     1321 accacatagg aagagctgat agtgtgcaga aacttgttga cggccacc
//
LOCUS       DAOSFP010000457         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_102709_length_1356_cov_4.733282, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000457 DAOSFP010000000
VERSION     DAOSFP010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102709_length_1356_cov_4.733282"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..686
                     /gene="ndk"
                     /locus_tag="PLI16_10540"
     CDS             255..686
                     /gene="ndk"
                     /locus_tag="PLI16_10540"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOH85038.1"
                     /translation="MAGNITLTIIKPLAFHNNKAVPILHRITEAGFHLVAAKTMHMTV
                     EQAKLFYQVHREREFYNNLVAFMSSGPVMVAMLQKENAVEDFRKLIGKTNPAEAAEGT
                     IRKDFGTSMQANAVHGSDSDENAQKECNFFFAECERFYQEG"
     gene            complement(674..1252)
                     /locus_tag="PLI16_10545"
     CDS             complement(674..1252)
                     /locus_tag="PLI16_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH85039.1"
                     /translation="MVDRILLVLKIKNLTPSKFADEIGIQRSSMSHIMSGRNMPSLDL
                     ITKILGRYPDINSDWLVKGEGPMTKTFQIDLFDQAAENKTPEATPKPKDDQPVTEPVN
                     DIKKEDVVEKSEENQLKTPVKETERVDIKPVEPVIQVPLQEEEKPAEKNTEQKASIGQ
                     AENTMALPANEPAIEKIVIFYKNRTFKEYYPS"
BASE COUNT          366 a          261 c          267 g          462 t
ORIGIN      
        1 ccggaaaatc gttaggtacc atggcacagg cattatattt ctttttgagg tatagccaca
       61 tggccagcac agagcccacc gcatcgccgt cgggattgtt atgcgagatt actacaatat
      121 tctttgctgc gctgattttt tgctgaacgt ctttaaaaaa ggtagtttcc aaggtgtttt
      181 ttttacaaaa ttattatttt ttgccgtgga taggcagatt ttattatttt tacagtaatc
      241 aatattttaa agttatggct ggcaatatca cgcttacaat aattaaaccc ctggcttttc
      301 acaacaacaa agcggtaccc atcttacacc ggattaccga agccggcttt cacctggtgg
      361 cggccaaaac tatgcatatg accgtagaac aggcaaaact gttttaccaa gtacacaggg
      421 aacgggagtt ttacaacaac ctggtggcct ttatgtcgtc gggacccgtg atggtagcta
      481 tgttgcagaa agaaaatgcc gtagaggatt ttcgtaaact tattggcaaa acaaatcctg
      541 ctgaagctgc tgaaggcaca ataagaaagg attttggaac cagcatgcag gccaatgccg
      601 tccatggctc cgatagcgat gaaaatgcac aaaaagaatg taacttcttt ttcgctgaat
      661 gcgaaaggtt ttatcaggaa ggataatatt ctttaaaagt tctgttttta taaaagatca
      721 cgattttttc gatagcaggc tcattcgcgg gtaatgccat ggtattttct gcctgaccaa
      781 tgctggcttt ttgttctgtg tttttttctg ccggtttttc ttcctcttgt agaggcacct
      841 gtataacagg ttcaacaggc tttatatcaa cacgttcagt ttcttttaca ggggttttta
      901 actgattttc ctctgatttt tcaacaacat cttctttttt aatgtcattt acgggctctg
      961 ttacaggttg atcgtccttt ggcttaggag ttgcctctgg agttttattt tctgctgcct
     1021 gatcaaataa gtcgatttga aatgttttcg tcatgggacc ttcaccttta accaaccaat
     1081 cggaatttat gtcaggatat cttccgagga tttttgtaat cagatccagg ctgggcatat
     1141 ttcgtcccga catgatgtgc gacatgcttg atcgttgtat gccgatctca tcggcaaatt
     1201 ttgatggtgt cagatttttt atcttaagca ccaataatat tctgtcaacc atattacaat
     1261 tataaatttt gacgcaacaa agataataca tttgtaattt acattcgtaa ttttctttag
     1321 tttatttatg taaactcatc tgtttagaaa aattat
//
LOCUS       DAOSFP010000458         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_103194_length_1352_cov_3.046261, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000458 DAOSFP010000000
VERSION     DAOSFP010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103194_length_1352_cov_3.046261"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="PLI16_10550"
     CDS             complement(<1..895)
                     /locus_tag="PLI16_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOH85040.1"
                     /translation="MRKAKKIFTPQVLQQVEITDAGAEGKAIGKTENLVVFVDNAIPG
                     DIVDIQLTRKKKSFAEGKVVQYHRLSEKRTEPFCKHFGMCGGCKWQQMQYEHQLFYKQ
                     KQVADNFTRIGHITIPDIRPILASPLQTCYRNKLEYTFSDYRWLTDEDMQSGSRDTMD
                     MNALGFHIPRHFDRVLDIETCYLQDEPTNIIRNRLKEFCIANGLTFYNPRKHTGFMRN
                     LLIRNSTIDETMAIVVFGEEDEKNQNKVLDYILEIFPALTSLMYVVNTKHNDIINDLE
                     IQCYHGKPYIEEVLGSLRFRIG"
     gene            complement(1063..>1352)
                     /locus_tag="PLI16_10555"
     CDS             complement(1063..>1352)
                     /locus_tag="PLI16_10555"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOH85041.1"
                     /translation="RIFYNRVQDANRFFSSGTEGWKTDRGMIYIIFGQPNSVYRSPYL
                     ETWNYGEDRNILSMTLDFNKTLNPFTENDYTLERSPEYKDVWYSAVEAWRK"
BASE COUNT          329 a          305 c          287 g          431 t
ORIGIN      
        1 gcccgatacg gaagcgcagg ctgcccagca cttcttcaat ataaggtttc ccatgataac
       61 attgtatttc caggtcgttg atgatatcat tatgcttagt atttacgaca tacatcaatg
      121 atgtaagtgc cggaaaaatt tccaggatat aatccagtac tttattttga tttttttcat
      181 cttcttcacc aaagactact atggccatag tttcgtcgat ggtggagttt cttatcagca
      241 ggttgcgcat aaaaccggta tgcttgcggg ggttgtaaaa agtaaggccg ttggcgatac
      301 aaaattcctt aagccggttt cttatgatat tggttggctc gtcctgcaaa tagcaggttt
      361 cgatgtcgag cacgcggtcg aaatgcctgg ggatatggaa acccagggcg ttcatatcca
      421 tagtgtcgcg gctgcccgac tgcatgtctt catcggtgag ccagcggtaa tccgaaaaag
      481 tgtattccag tttgttgcgg tagcaggttt gcagcggtga ggcgaggata gggcggatat
      541 ccggtatggt gatatggccg atacgggtaa aattatctgc cacctgtttt tgtttataaa
      601 acaactggtg ttcgtattgc atctgttgcc atttgcagcc gccgcacatt ccaaaatgtt
      661 tgcaaaatgg ttcggtacgt ttttcggata agcggtgata ttgcacaacc ttgccttcgg
      721 caaaggattt ctttttcctg gttagctgta tgtccacaat atcgcctgga atcgcgttgt
      781 caacaaagac taccaggttt tcggttttgc ctatggcttt gccttcggcg ccggcatcgg
      841 taatctctac ctgttgcagc acctgtggtg taaaaatttt tttcgctttt ctcactatta
      901 tcaattatca atcaacaatg aacaattatc tgtgaacaac aaccaatgat ttatgattat
      961 ggccactttc cactttatac tttcaacttt taacttttaa ctttcaactt gccactttcc
     1021 actttccact ttccacttgc tacttccctc ttccctcttc ccttatttcc tccaagcttc
     1081 caccgcactg taccacacat ctttatattc cggactgcgt tccagggtat agtcattttc
     1141 tgtaaaagga tttaaggtct tattaaaatc gagagtcatg gataaaatgt tgcggtcctc
     1201 gccataattc catgtttcga gatagggtga tctgtacacg ctgttaggct gaccgaagat
     1261 gatataaatc atgccccggt cggttttcca tccctcggtg ccggacgaaa aaaaacggtt
     1321 ggcatcctgc accctgttat aaaaaattct ga
//
LOCUS       DAOSFP010000459         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_106146_length_1327_cov_3.491352, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000459 DAOSFP010000000
VERSION     DAOSFP010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106146_length_1327_cov_3.491352"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLI16_10560"
     CDS             complement(<1..398)
                     /locus_tag="PLI16_10560"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOH85042.1"
                     /translation="MKNRCPWPADDAQMMTYHDTEWGVPVHDDHKLFEFIVLDAFQAG
                     LSWKTILHKRQAFRKAFDNFDPVKIAKYDDKKIAELLENPGIIRNRRKITATIKNAQH
                     YLEIIEEYCSFDTFIWQFTQHKMKVNKCKT"
     gene            complement(429..>1327)
                     /gene="ribA"
                     /locus_tag="PLI16_10565"
     CDS             complement(429..>1327)
                     /gene="ribA"
                     /locus_tag="PLI16_10565"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HOH85043.1"
                     /translation="RAKTIYALSKQETKPEELGRPGHIFPLRAKDGGVLRRAGHTEAT
                     VDMARLAGLKPVGALVEIMNEDGSMARLMDLYKIRDKYHLKMVSIKDLIAYRIKNESL
                     IEKEETVDLPTEWGNFKLSLFREITTGQHHMALSKGTWEKDEAILVRVHSSCMTGDVF
                     GSCRCDCGAQLHTAMKMIEKEGKGLIIYMNQEGRGIGLANKLKTYHLQELGYDTIEAN
                     VKLGFKPDERDYGIGAQIIRNMGAGKIKLITNNPRKKSGLMGYGIEIVENIPIVIKPN
                     QYNKYYLKTKQDKMGHILNIDD"
BASE COUNT          282 a          295 c          299 g          451 t
ORIGIN      
        1 aatgttttac atttattcac tttcattttg tgttgagtga attgccatat aaaagtatca
       61 aaactgcaat attcttcaat tatttcaaga taatgctgtg cattttttat ggttgcagta
      121 atttttcgcc tgttgcgtat gatgcctgga ttttctaata attcagcaat ttttttatcg
      181 tcgtattttg caattttcac agggtcaaaa ttatcaaaag ccttacgaaa agcctggcgt
      241 ttgtgaagta tggttttcca gctcaggccg gcttgaaaag catcaagcac tataaattcg
      301 aataatttat ggtcgtcatg tactggcact ccccactcgg tatcgtggta ggtcatcatt
      361 tgcgcatcat cggcgggcca gggacagcgg tttttcatgg aaaagagtct tgtttcaggt
      421 agctcaaatc agtcgtcaat attcaggata tggcccattt tgtcctgctt ggtcttgagg
      481 taatacttgt tgtattggtt gggttttatc actatgggga tgttttccac tatctcgatt
      541 ccgtatccca tgagtccgct ttttttccgg ggattgttag tgatgagctt aattttgccg
      601 gcacccatat tgcgtatgat ctgcgctccg ataccatagt cgcgttcatc gggcttaaat
      661 cccagtttta cgttagcttc aatggtgtca tacccgagtt cctgcaggtg atatgttttc
      721 aatttgttgg ccaggcctat gccgcgtcct tcctggttca tatatattat caggcctttg
      781 ccttcttttt ctatcatctt catggcggta tgcagttgtg ctccgcaatc gcagcggcac
      841 gagccaaaaa catcgcccgt catgcatgaa gagtgaacgc gtaccaggat agcttcatct
      901 ttttcccagg tgcctttgga tagtgccatg tgatgctggc ctgtggtaat ttcgcggaat
      961 agcgaaagtt tgaagttgcc ccattcggtg ggcaaatcca ccgtttcttc tttttctatc
     1021 aggctttcgt tttttatacg gtaggcgatg agatctttga tggagaccat ttttaaatgg
     1081 tacttatccc tgattttata aaggtccata agccttgcca tggagccgtc ttcgttcata
     1141 atttcgacca gggcgcccac aggttttaat cctgccagcc ttgccatgtc gacggtggct
     1201 tcggtatgcc cggcacgcct gagcaccccg ccgtctttgg cacgcagcgg aaagatatgc
     1261 cccggtcttc ccagttcttc gggttttgtt tcctgtttcg aaagcgcata gatggtttta
     1321 gccctgt
//
LOCUS       DAOSFP010000460         1317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_107367_length_1317_cov_4.280507, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000460 DAOSFP010000000
VERSION     DAOSFP010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107367_length_1317_cov_4.280507"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLI16_10570"
     CDS             <1..493
                     /locus_tag="PLI16_10570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOH85044.1"
                     /translation="IQIIPVYSSPVVDLGPDTSICTGCSITLDAGNPGAEHWWSTGDS
                     TQQITISGADTVWVAVSVHGCSGYDTIVVAEDTVTGIGLKAPHNFDIAPNPNSGVFRI
                     NTNNTENYFVEVWSADGRCLLTAGPYFGNASVDLTAAGTGFYVAIIKNSERVVAKKKL
                     LIY"
     gene            complement(539..>1317)
                     /locus_tag="PLI16_10575"
     CDS             complement(539..>1317)
                     /locus_tag="PLI16_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH85045.1"
                     /translation="KIKNNEMMKKILLLVVIIIQGLLASAADNKEIIARANQAYTEEK
                     YTEAIELYQQVVKNNFQSSELYYNLGNAYFKTKDYPSALLYYEKAKKLDPSGEKIDFN
                     INVANTKIIDKIEELPQPFYKRWLTGFRQSLSVDTWAVLSLIFLFVFFTAAALYLIGN
                     TVTIRKKFFWVGVVFFTGALISGISAYHQHRDFQTAREGIVFEPTVNVKSSPDEGSQD
                     IFVIHEGTKVKITDKVGTWIEIRIANGSDGWVPESVIRII"
BASE COUNT          351 a          269 c          304 g          393 t
ORIGIN      
        1 catacagatt attccggttt attcttcgcc tgtggtggat ttaggacctg ataccagcat
       61 ttgtacgggt tgcagtataa cgcttgatgc gggcaatccg ggtgctgaac attggtggtc
      121 aacgggcgac agtacacagc agataacgat ttccggtgca gatactgtct gggtggcggt
      181 ttctgttcat gggtgttccg gctatgacac tattgtggtt gctgaagata ccgttaccgg
      241 tattggctta aaggcaccgc ataattttga catcgcaccc aatcccaatt cgggggtttt
      301 caggataaac accaacaaca cggaaaatta ttttgtggag gtttggtcgg ctgatggtcg
      361 ttgcctgcta acagccgggc catatttcgg taatgcttct gtagatctga cagcagcggg
      421 gacgggattt tatgtagcaa taatcaaaaa tagcgaaagg gttgtggcga agaaaaagtt
      481 acttatctat tgacactata ggaatttttg atgacactca ggaaatccac agattccttc
      541 aaataattct tatcaccgat tcgggtaccc agccatcgct gccgttggca atcctgattt
      601 ctatccaggt gcctacctta tcggtgattt taacttttgt gccttcgtgg ataacaaaaa
      661 tatcctgact gccctcgtcg ggagagcttt tcacatttac ggtaggttcg aagacgatac
      721 cttcacgggc ggtttggaag tcgcggtgct gatggtacgc actgataccg gaaatcaaag
      781 cgcctgtaaa gaaaacaaca ccaacccaaa aaaatttctt acgtatggta acagtgttgc
      841 caataaggta aagggcggct gccgtgaaaa atacaaacaa aaatatcagg cttagtaccg
      901 cccaggtgtc aaccgaaagg ctttggcgga accccgtcaa ccatcgtttg tagaatggtt
      961 ggggcaactc ttctatttta tcaatgattt tggtatttgc cacgttgata ttaaaatcaa
     1021 ttttttcgcc ggaaggatcg agcttttttg ctttttcata atacaacaat gccgaagggt
     1081 aatctttggt tttaaaataa gcattgccga gattgtaata caattccgaa gactggaaat
     1141 tgttttttac cacttgctga taaagttcaa tggcttcagt gtatttttcc tccgtatagg
     1201 cctggttggc acgggcaatg atttctttat tgtcagccgc tgaagccagc agcccctgaa
     1261 taatgataac gacaagtagc aagatctttt tcatcatttc attatttttt attttta
//
LOCUS       DAOSFP010000461         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_107638_length_1315_cov_3.421429, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000461 DAOSFP010000000
VERSION     DAOSFP010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107638_length_1315_cov_3.421429"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLI16_10580"
     CDS             complement(<1..536)
                     /locus_tag="PLI16_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008672475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOH85046.1"
                     /translation="MKIALVTGGSRGIGRAVCLKMAAMGYHVLVNYLSGKEAAEETLA
                     GITQNGGTGELLPFDISKQESIESALEQWYKNNPDKYIEVLVNNAGIRKDALLMWMKN
                     EEWEQVMNTNMNGLFFITRRLIKDMLINKYGRVVNIVSLSGIKGLPGQVNYSAAKAGV
                     IGITKALAQEVGKKNVTV"
     gene            complement(721..>1315)
                     /locus_tag="PLI16_10585"
     CDS             complement(721..>1315)
                     /locus_tag="PLI16_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85047.1"
                     /translation="DALTNGHAVWVMGNDDAHGISDGSNGVCWTMINTDSLSGESITK
                     SLQQGNFYATRGWLGQEMNRIISVTVTGNTYELLLEKASDSIILKSNWGQTVKSETGK
                     NKISYDIKPSDSYIRAEIYDTEPWNGYTRIYLNPVIRTPEGSLIKHNNIAGINVIKTI
                     SFVLLLLVLHAFGLYILYVINFKKDKKTSQADNKPKN"
BASE COUNT          317 a          274 c          259 g          465 t
ORIGIN      
        1 ttaacagtaa catttttttt gcctacttcc tgtgccagag ccttggtaat gcctatcacg
       61 ccggctttag cggcagaata atttacctgt cccggtaagc cttttatgcc cgaaagggat
      121 actatattga ctacccggcc atatttgttt atgagcatat cctttatcag gcggcgggta
      181 atgaaaaaaa gaccgttcat gttggtattc attacttgct cccattcttc gtttttcatc
      241 cacatcagca gggcatcttt tcttatgccc gcgttgttga ccagcacctc gatatattta
      301 tccggattgt ttttatacca ctgctccaat gcgctttcaa tactttcctg cttcgaaatg
      361 tcaaaaggca gcaattcgcc tgtaccgcca ttttgggtta tcccggccag ggtttcttcg
      421 gctgcttctt tgcccgagag ataatttaca agtacatggt atcccattgc agccatttta
      481 agacatacag ccctgccaat gcctcttgaa cctcctgtga ccaatgctat tttcatattt
      541 gtttcgttgt tatccaatag ttatgttgtt gtggtaatta tcagtatggt tttatgataa
      601 ttacatttta ttctaaatga ttgaaattta attaaatgtg tgaaaaatat tttttattaa
      661 tagggtgcaa agataatata atttttttta ccgtaataaa atttttgggg gagaaaggcg
      721 tcaatttttt ggtttgttat ctgcttgtga agtcttttta tctttcttaa aattaatgac
      781 atacaaaatg tacaaaccaa acgcatgcaa caccagcagc aggaggacaa aacttatggt
      841 tttaattaca tttattccgg ctatgttgtt gtgctttatc aatgagccct caggagttct
      901 gataacaggg tttaaatata tcctggtata gccgttccag ggctccgtgt cgtatatttc
      961 ggccctgata taagaatcgg atggctttat gtcgtatgag attttgtttt tgcctgtttc
     1021 gctttttact gtttgtcccc aattgctttt taagatgatg gaatcggaag ctttttccag
     1081 cagcagttcg taagtgtttc cggtgaccgt gacactaatt atgcggttca tttcctgccc
     1141 gagccatcct cttgtggcgt aaaaattgcc ttgttgtaaa ctttttgtaa tggattcccc
     1201 gctcaggcta tcagtgttta tcatggtcca gcatacgccg ttgctgccgt cgctaatgcc
     1261 gtgagcatcg tcgttgccca taacccatac agcatggccg ttagtaagag cgtct
//
LOCUS       DAOSFP010000462         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_112349_length_1279_cov_2.998366, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000462 DAOSFP010000000
VERSION     DAOSFP010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112349_length_1279_cov_2.998366"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLI16_10590"
     CDS             <1..599
                     /locus_tag="PLI16_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85048.1"
                     /translation="AEIGAIFLLKKVPVPMVWLIVLIVVDMILAIIGSLQWKKANRFD
                     PASEKDKIRFFIQNQLGAIISIIAFLPLIILVFTNKNMTGKQKGIAGAVGIVALLVAG
                     YFGIDFNPPSQEQYQQQTEQVKQLNEGQDFVYWTKSGTRYHLYKDCPYINTDRTDEIF
                     EGTVETARHEKNITELCSYCENRAKKENEVLPVTDSVK"
     gene            973..>1279
                     /locus_tag="PLI16_10595"
     CDS             973..>1279
                     /locus_tag="PLI16_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85049.1"
                     /translation="MKKRQPKIFQKIIFCSLLFFIIFSGTNLKAQTPTIKWWYDLHDA
                     SFGQTAAGDIDNDGKLELVFGCYRNDSCVYALNSEDGSLLWKYNTHPAGAEGCNDVAP
                     "
BASE COUNT          406 a          260 c          269 g          344 t
ORIGIN      
        1 gagccgaaat aggcgccata tttttactta aaaaagttcc cgttcctatg gtatggctga
       61 tcgtgctgat agtggtggat atgattttgg ccattatcgg ctcactgcag tggaaaaagg
      121 ccaacaggtt tgaccctgca tcggaaaaag ataagataag attttttatt cagaaccagc
      181 ttggcgccat catcagcatt atagcgttcc tgccactcat tattctcgta tttaccaaca
      241 agaatatgac aggaaaacaa aaaggtattg ccggtgcagt aggtattgtg gcattgctgg
      301 tagcaggata ttttggcatc gatttcaacc cgccatcaca ggaacagtat caacagcaaa
      361 ccgaacaggt aaagcagttg aatgaaggcc aggactttgt gtactggaca aaatccggta
      421 cacgctacca tttgtataaa gattgtccgt atatcaacac cgacaggaca gacgaaatat
      481 ttgaaggcac tgtggagaca gcaaggcatg aaaagaatat cactgaatta tgcagttatt
      541 gtgaaaacag agccaagaag gaaaatgaag tactgcctgt cacggatagt gtcaaatagt
      601 aacgcagggt tttatttcaa ctgctaagct attaacaata aaaggatttt agcctaaaca
      661 gccggagggt gacgtgatgc aacctccggc tgtttttatc aggcatgcag ccgcatacca
      721 tctttaaaaa atacgttttg cacatatttt atatgaaatt ctgcactata aacaatttgg
      781 aatgccagtg aacatttcct aagtcggtta ttcagccgca ggattaaccc caacctgagc
      841 aataagccct aaactcctat tgccgaattt gttaaaatgt ttttcttttt gattaagaat
      901 ccttaatttt gagaatacct ttaataaacg gtcgcattac ttataaataa cctatcagga
      961 gagcaaaaca acatgaaaaa acgacaacca aagatttttc aaaagattat tttttgtagt
     1021 cttttgttct tcatcatttt ttcaggcact aacctgaaag cacaaacgcc cacaatcaaa
     1081 tggtggtatg acttgcatga cgcctctttc gggcaaacag cagccggcga cattgacaac
     1141 gacggaaagt tggaactcgt ttttggatgt taccgcaacg acagttgtgt ttatgcatta
     1201 aactccgaag acggttcgct gctatggaaa tacaatacgc atccggccgg cgccgaaggg
     1261 tgcaatgacg tggcccctg
//
LOCUS       DAOSFP010000463         1270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_113555_length_1270_cov_3.994239, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000463 DAOSFP010000000
VERSION     DAOSFP010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113555_length_1270_cov_3.994239"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /gene="amrA"
                     /locus_tag="PLI16_10600"
     CDS             <1..625
                     /gene="amrA"
                     /locus_tag="PLI16_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOH85050.1"
                     /translation="SLRVVGYNAMAVSGIIKKKMNEEFSLSSKDKEDLLDIARNTLTE
                     FIKNGKIPAIDTAGFSGVIKQNCGAFVTLHKKGQLRGCIGRFEADKPLYLVIQDMAIA
                     ASTEDYRFSRVVADELKLIDLEISVLSPLKLIKSIDEIELGKHGIYIKKGNRGGTFLP
                     QVATETGWTKEEFLGHCAEDKAGLGWYGWKDADIYINSAIVFGEKDE"
     gene            770..>1270
                     /locus_tag="PLI16_10605"
     CDS             770..>1270
                     /locus_tag="PLI16_10605"
                     /inference="COORDINATES: protein motif:HMM:NF021210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2400 family protein"
                     /protein_id="HOH85051.1"
                     /translation="MNTSELRELLDEKYDEYCTPAFIDDDPISVPHYFSKKEDIEIAG
                     FFAAMLAWGQRQQIIKSARNLMQMMDYAPFDFLTGATKKELNPFKSFVYRTFNGNDCV
                     FFLHSLKNIYLKHQGLEAVFIEGYRRYGDLFAAIGMFRTVFFETAHLPRSEKHISDPA
                     RNSACKR"
BASE COUNT          382 a          270 c          303 g          315 t
ORIGIN      
        1 tagcctgcgg gtggtaggct ataacgccat ggcggtgagc ggcataatca agaaaaaaat
       61 gaacgaagaa ttttcgttga gcagtaagga caaggaagac ctgttggata tagcccgcaa
      121 caccctgaca gagtttatca aaaatggtaa aattccagct attgacacag ctggtttttc
      181 aggtgttatc aaacaaaact gcggcgcttt tgtaacactg cacaaaaaag gtcagctacg
      241 gggctgtatc ggaaggttcg aggctgataa acccttgtat ctagtgattc aggatatggc
      301 catcgctgca tcaacagagg attacaggtt tagccgtgtg gtcgccgacg aattaaaact
      361 tatcgacttg gagatttccg tgttgtcgcc gctgaagctc ataaaatcca tcgatgaaat
      421 agaacttggc aaacacggca tctacataaa aaaaggcaac cgcggcggca catttctgcc
      481 gcaggtagcc accgaaacag ggtggacaaa agaagaattt ctcggccatt gtgccgaaga
      541 caaagccggg ctgggatggt acggatggaa agatgccgat atttacatca attcggctat
      601 cgtctttggg gagaaggatg agtaatagct taaaaacaat cattaccccg gtagccggaa
      661 gttttcaaaa atcatgttta tcaaactaat ctgactaaaa agtaactgaa aaaatacctg
      721 aagcggctgt ccctgcttat agttgctgct tccctgtaaa gccgacacta tgaatacctc
      781 cgaactacgt gaactgctgg atgaaaaata cgatgaatat tgcacgccgg cttttattga
      841 tgatgaccca atcagtgtgc ctcattattt ttcaaagaaa gaagatattg aaatagcagg
      901 gttctttgcg gccatgcttg cctggggcca aagacagcag atcataaaaa gcgccaggaa
      961 cctgatgcag atgatggatt atgcgccctt tgattttttg accggcgcca caaaaaaaga
     1021 actgaaccct ttcaaaagct ttgtgtaccg gactttcaac gggaatgact gtgttttttt
     1081 cctgcattcg ttaaaaaata tttacctgaa gcaccagggt ttagaggctg tttttatcga
     1141 gggttaccgc cgctatggag acctgtttgc ggccatcggt atgttcagaa ctgtattttt
     1201 tgaaacggca cacctgccgc gcagcgagaa acatatttcc gaccccgcaa gaaattccgc
     1261 gtgtaaacgc
//
LOCUS       DAOSFP010000464         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_118514_length_1236_cov_4.011854, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000464 DAOSFP010000000
VERSION     DAOSFP010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118514_length_1236_cov_4.011854"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..>1236
                     /gene="rocD"
                     /locus_tag="PLI16_10610"
     CDS             74..>1236
                     /gene="rocD"
                     /locus_tag="PLI16_10610"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOH85052.1"
                     /translation="MEETLKTNTATQAAIALEDKYGAHNYHPLPVVLNKGEGVFVWDV
                     EGKRYFDFLSAYSAVNQGHCHPKIINALIDQARTLTLTSRAFYNDCLGPYEKFITEYF
                     GYDKVLPMNSGAEGDETALKLCRKWAYKVKGIPENQAKIICCENNFHGRTITIVSMST
                     DPDATNDFGPFTPGFVKIPYNDLKALEIALEDPNVAGFLVEPIQGEAGVFVPDEGYLA
                     KAFALCKAKNVLFIADEVQTGIARTGKMLCCDHENVKPDILILGKALSGGVMPISAVL
                     ANDDIMMTIKPGEHGSTFGGNPLAAKVAVAALQVVKEENLCERAEKMGKIFREEIKKI
                     DSDMIELVRGKGLLNAVIIRPKNGKEAWDVCLKMRDNGLLAKPTHGHIIRFAPP"
BASE COUNT          391 a          267 c          278 g          300 t
ORIGIN      
        1 agtgtaatac ggattttgga acaacaaaat tactatattt gccactggaa atgctcattt
       61 aaaacccaaa tatatggaag aaacattaaa aacaaacaca gctacccagg cagctattgc
      121 acttgaagac aagtacggtg cacacaacta tcatccattg ccggtagtat taaacaaagg
      181 cgaaggtgtc tttgtttggg atgtagaagg aaaaaggtat tttgatttcc tgtcggcata
      241 ctctgcagtc aaccagggac attgccatcc gaaaattatc aatgcactga ttgaccaggc
      301 acgtacgctg accctgactt cccgtgcgtt ttacaacgac tgcctggggc cttatgaaaa
      361 attcattacc gaatattttg gctacgataa ggtactgcca atgaattcag gcgccgaagg
      421 tgatgaaaca gccctgaaat tatgccggaa atgggcttat aaagtgaaag gtattcccga
      481 aaaccaggca aaaatcattt gctgcgaaaa caattttcat ggaagaacta tcaccatagt
      541 atccatgtca accgaccctg atgccaccaa tgactttggg ccttttacgc ccggttttgt
      601 aaaaattcct tataacgacc tgaaagcact ggaaatagca ctggaagatc ctaatgttgc
      661 cggattcctt gtggagccta tacagggcga agccggtgtt tttgtccccg acgaaggcta
      721 tctggccaag gcgtttgctt tatgcaaagc aaaaaatgtt ttattcatag ccgacgaagt
      781 acagacaggt attgcacgaa ctggaaaaat gctgtgctgc gaccacgaaa atgttaaacc
      841 agacatcctt attcttggaa aagcattgtc aggtggcgtg atgccaatct cggcagtatt
      901 agccaatgat gatatcatga tgactataaa acccggcgaa catggttcca ccttcggcgg
      961 caacccgctt gctgcaaaag tagctgtagc ggctttacag gtggtgaaag aagaaaacct
     1021 ctgcgaacgc gccgaaaaaa tgggaaaaat tttccgtgaa gaaataaaaa agattgattc
     1081 ggatatgata gaactggtac gcggaaaagg tttgctcaac gctgtcatca taagacctaa
     1141 gaacggcaaa gaagcctggg atgtttgttt aaaaatgcgc gacaatggct tgctggccaa
     1201 acccacgcat ggacacatca ttcgctttgc accgcc
//
LOCUS       DAOSFP010000465         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_119346_length_1231_cov_3.221088, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000465 DAOSFP010000000
VERSION     DAOSFP010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119346_length_1231_cov_3.221088"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLI16_10615"
     CDS             complement(<1..928)
                     /locus_tag="PLI16_10615"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOH85053.1"
                     /translation="MKKSVLFLSVLLMMAASCSKPQKTNLVNYKGTVQGSFFLIKFYA
                     GTDSNTIKSSIDSLFVVIDQTASVFDSNSIISKVNDNRETALNPHFIALFNKSQEVSA
                     ATGGCFDITVGPLVKSWGFWKKQGLEMDKKKVDSLLLCVGYQKVRLVNTGKGSNLLKE
                     NPCIMLDFNAIAQGYTADVIAEYFNKKGSSNYLVEIGGEVRASGTKKDNTNWMVGIEK
                     PADSSNAEQVVYEKVELINKSLATSGNSRKFFIKDGIKYSHTIDPATGYPVHHSLLSV
                     TVMADDCTSADAYATAFMVMGLEKAKEYIKTHA"
     gene            complement(996..>1231)
                     /locus_tag="PLI16_10620"
     CDS             complement(996..>1231)
                     /locus_tag="PLI16_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOH85054.1"
                     /translation="EKVLVIMTQKMGFLAVFLAYVLPFLVMIALMLVADYKEASEPIT
                     GLLVVVFLTAYFFSLYAFRKKLNRKFTFTIKKL"
BASE COUNT          338 a          254 c          254 g          385 t
ORIGIN      
        1 ctgcgtgagt ttttatatac tctttggctt tttcgagacc cataaccata aaggcggtgg
       61 cataggcatc cgccgaagtg cagtcgtcgg ccatcaccgt tacgctcagc agcgaatgat
      121 gaacgggata acccgtagcg gggtcgatgg tgtgtgaata tttgatgccg tctttaataa
      181 aaaatttgcg tgaatttcct gatgtggcca gtgatttgtt tatcagttcc actttttcat
      241 ataccacctg ttcggcattg ctgctgtcgg cgggtttttc gatgcccacc atccagttgg
      301 tattgtcttt ttttgtcccg gaagcccgca cttcgccgcc tatttcaacg agataattgc
      361 tggaaccttt tttattgaaa tattcagcaa tcacatccgc tgtgtagccc tgtgctatgg
      421 cattaaaatc cagcatgata caaggatttt ctttaagcag gttgctccct tttccggtat
      481 taaccagccg gactttttga tacccgacac aaagcaataa gctgtcaacc tttttcttat
      541 ccatttcaag gccttgtttc ttccaaaaac cccaggattt taccaggggc ccaacggtga
      601 tatcaaaaca tcctccggta gctgctgaaa cttcctgcga tttattgaaa agtgcaatga
      661 aatgagggtt gagggctgtt tcacggttgt cgttaacttt ggaaataatg gaattactat
      721 cgaaaacgga agctgtttgg tcgataacaa caaacaatga gtcaatggat gattttatag
      781 tattgctgtc agtcccggca taaaatttaa tcaaaaaaaa ggagccttgc actgtccctt
      841 tataattaac cagatttgtt ttttggggct tactgcatga ggcagccatc atcagcagta
      901 cacttaagaa taatacactc tttttcattt ttcaggtatt ttaataattg gttttttgag
      961 ggaattgacc ggccgataca ttatgtaaat gcgtatcaca attttttaat ggtgaaggta
     1021 aattttctgt ttaatttttt tcggaaggca tacagcgaaa aaaaatatgc tgtcagaaac
     1081 accaccacca aaaggcctgt tatgggttct gaggcttctt tataatctgc aacaagcata
     1141 agggcaatca taacaaggaa gggaaggaca tatgccagaa aaactgccag aaaccccatt
     1201 ttttgcgtca tgattacaag tactttttct c
//
LOCUS       DAOSFP010000466         1229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_119691_length_1229_cov_2.425894, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000466 DAOSFP010000000
VERSION     DAOSFP010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119691_length_1229_cov_2.425894"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1229
                     /locus_tag="PLI16_10625"
     CDS             <1..>1229
                     /locus_tag="PLI16_10625"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOH85055.1"
                     /translation="NITCKFDDNYFAGTYDLILRNLPFFLDREDYDPIEKMLTPEHID
                     SVMLSNYKLLLSPAGTFLKKQIVSDPLNIGSLAMEKMRMPGIGSNFRIYKNHIFSQDL
                     RHLLMFVQSSYPANESFHNKKLIKELDNIIREHKANTGRESLTIQYYGTVAASVGNSV
                     QLKKDVVVTLGIAILILFIFFSVFFRKKTTFILVLIPVVFGGLFSMAILSLLNDDISL
                     IALGAGSIILGVALTYSIHFFSHYRHTGSVRQVIKDLSLPLTLGSVTTIGAFLGLLFV
                     RSEVLNDFGLAAAMCLLGSVLCTLIFLPFIIKHKNEETGGPSKTNIIDRISAYHPERN
                     PYLIVALAVITVFLFFMAGKPGFETDMNTLGYTPEKLRQAETTLQHISGDASRQVFLI
                     SKGDNLNAALQHNENAC"
BASE COUNT          322 a          297 c          265 g          345 t
ORIGIN      
        1 aaaatattac ctgcaagttt gacgacaatt attttgccgg cacctatgat ttaattctca
       61 gaaatcttcc ttttttcctt gacagagaag attatgatcc tatagagaaa atgctgactc
      121 cggaacatat tgactcggta atgctcagca actataaact actgctttcc cctgccggaa
      181 ccttcctgaa aaaacaaatt gtcagcgacc cgctcaacat tggcagtttg gcaatggaaa
      241 aaatgcgcat gcctggcatt ggcagcaatt tcaggattta taaaaaccat attttttcgc
      301 aggacctccg ccacctgttg atgtttgttc aatccagtta tcccgccaac gaaagttttc
      361 ataacaaaaa gctcatcaaa gaacttgaca atatcatccg ggagcataag gcaaataccg
      421 gcagggaatc gcttaccata cagtattacg gaacggtagc ggcttccgtg ggaaactccg
      481 tgcaactaaa gaaagacgtg gtcgttaccc tgggtattgc gatactgata ttattcattt
      541 ttttcagtgt gttctttcgc aaaaaaacca ctttcatcct ggttttgata ccggtggttt
      601 ttggcggtct gttttccatg gccatcctgt cgctgcttaa cgatgacatc tcgctcatcg
      661 ctctgggagc cggatcaata atccttggcg tggcgctgac ttattccata catttttttt
      721 cgcattaccg tcatacaggt tcggtccgac aggtcatcaa agacttatct ttgccgctga
      781 cacttggcag cgtaaccact ataggcgcgt ttttggggct cttgtttgtc aggtcggaag
      841 ttctcaacga tttcggcctg gccgctgcga tgtgcctgct ggggtcggta ctgtgtacct
      901 tgatattttt gcctttcata ataaaacaca agaatgagga aacaggcggc ccttcaaaga
      961 cgaatatcat cgacaggata tcggcttacc accccgaacg gaatccttat ctgatagtgg
     1021 ctctggctgt catcacggtg tttttgtttt ttatggccgg gaaacccggc tttgaaaccg
     1081 acatgaatac actcggctac accccggaaa aactgagaca ggccgaaact actcttcagc
     1141 atatttcggg agatgcttca cgccaggttt tcctgatcag caaaggagat aacctgaacg
     1201 ccgcgttgca acacaacgaa aatgcctgc
//
LOCUS       DAOSFP010000467         1210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_122510_length_1210_cov_4.204329, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000467 DAOSFP010000000
VERSION     DAOSFP010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122510_length_1210_cov_4.204329"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLI16_10630"
     CDS             <1..387
                     /locus_tag="PLI16_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85056.1"
                     /translation="HIAGINLNKVARSTSSRGPGGHNNKGILLGACDVGKNLEEFMLN
                     CGSRISWVAGYDRETPWLEGTISDLLFLEYKLKGRIKTDATGEFIKDNDDQFIFAHAR
                     KAARIARWVLEDYPLAAKCGFRAIDR"
     gene            439..>1210
                     /locus_tag="PLI16_10635"
     CDS             439..>1210
                     /locus_tag="PLI16_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOH85057.1"
                     /translation="MSKISGLKLPSNKAIWALTYPIILSLFAENIINVTDTIFLARVG
                     EAELGASAIGGTFYVLLFIIGLGFSIGTQIIISRRIGENKEGEVGSVFEISVMFLMIA
                     AVLVFLATQLFFDPLFSRILSSGEILAKTELYVKWRVWGVFFTYIGCVFRAFYTGLFK
                     TKFLLANSLVLAAVNVLFAYVLIFGKFGFPQMGIEGAAIATVIAEGASVLFYLLVTLK
                     FVDLKKYRLFQPKKYDLKVIRTILDVSGFIILQNVLSMA"
BASE COUNT          286 a          250 c          310 g          364 t
ORIGIN      
        1 catatcgccg gtatcaacct gaataaagtg gcgcggtcca catcaagccg tggcccggga
       61 gggcataaca acaagggaat acttctggga gcctgtgatg taggtaaaaa cctcgaagaa
      121 tttatgctta actgcggttc gcgtatcagc tgggttgccg gctacgacag ggaaacaccc
      181 tggcttgagg gaaccatctc ggacttgttg tttctggaat ataaattaaa aggcaggata
      241 aaaacagacg ccaccgggga gttcattaag gataatgacg accagtttat ttttgctcat
      301 gcacgcaagg cagcgcgtat tgcccgttgg gtgctggagg attatccgct ggctgcaaaa
      361 tgtggttttc gcgctatcga cagataaaga ttcctgttga ctatttagtg tgcttacttg
      421 tgcaaacaat cgttactttt gtcgaaaatt tccgggttga aactgccttc aaataaagcc
      481 atctgggcgc tgacatatcc tattatcctc agtctttttg ccgagaatat catcaatgtt
      541 accgacacca tttttcttgc cagggtgggt gaggcagagc tgggcgcttc ggccatcggg
      601 ggcacttttt atgtgctgct ttttattatc gggctgggtt tcagcatagg cacacaaatc
      661 atcatttcgc ggcgtatagg cgaaaacaaa gaaggcgagg tgggcagcgt cttcgaaatc
      721 agtgtaatgt ttctgatgat agcggctgtg ctggtttttt tggctacgca actttttttt
      781 gacccgcttt tcagccgcat actctcgtcg ggagaaattc ttgcaaagac ggaactgtac
      841 gtcaaatggc gggtttgggg cgtgttcttt acatatatcg gctgtgtttt cagggctttt
      901 tataccggat tgtttaaaac aaagtttttg ctggccaatt cgctggtact ggctgcagtg
      961 aatgtactgt ttgcctatgt gttgattttt ggcaagtttg gttttcccca aatgggtata
     1021 gaaggcgctg ccatcgctac tgtgatagcc gagggcgctt cggtgttgtt ttaccttttg
     1081 gttactttaa aatttgtgga cctgaaaaaa taccgcttgt ttcaacccaa aaaatatgac
     1141 ctgaaggtga tccgcaccat acttgatgtg tccgggttta tcattcttca gaacgtattg
     1201 tccatggcat
//
LOCUS       DAOSFP010000468         1196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_124683_length_1196_cov_3.936021, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000468 DAOSFP010000000
VERSION     DAOSFP010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124683_length_1196_cov_3.936021"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="PLI16_10640"
     CDS             complement(<1..507)
                     /locus_tag="PLI16_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOH85058.1"
                     /translation="MAKKIRILSIDGGGIKGILPGIILEYIENQLRKQEGDDRRLSDY
                     FDLIAGTSTGGILTSIYLTPGEHSRPKYTASEAVDLYIKKGHEIFDIDFWHRIQSLGG
                     LTEEKFPVANLEKNLKTYLGNTKLSELSKPCLITAYEITKRAAFFFNSVNGKKSDLDD
                     FYVRDICRT"
     gene            complement(771..>1196)
                     /locus_tag="PLI16_10645"
     CDS             complement(771..>1196)
                     /locus_tag="PLI16_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOH85059.1"
                     /translation="AAGNPRSTAAHLSFILLIATFNVIGSLSMLIIDKRKDIAVLKSL
                     GAGNQTIRNIFFTEGMMISLSGALLGLVLGGVVCWIQQTFGVITISTGDTFVMDAYPI
                     RILALDFVYVFLIVCIIGFIAAWYPVKYISKKHLEQRLS"
BASE COUNT          369 a          257 c          227 g          343 t
ORIGIN      
        1 agtccggcat atatctctca cataaaaatc gtccaggtcg cttttttttc cgttcaccga
       61 gttgaagaaa aacgctgccc ttttggttat ttcgtaagct gtaatcagac aaggttttga
      121 caactcactg agtttggtgt tgcctaaata agttttaagg tttttttcaa ggttggcaac
      181 cggaaatttt tcttcagtca atcctcccag actttgtatc ctatgccaga aatcgatatc
      241 gaaaatctca tggccttttt ttatgtatag gtcaacggct tcactggcag tatatttagg
      301 acgactgtgc tctccggggg taagataaat actcgtcagg atacctcccg tgctggtgcc
      361 cgcaataagg tcgaaataat ccgaaagtcg tctgtcatca ccttcctgct ttcttaactg
      421 attttcaata tactcaagaa ttatcccggg caatataccc ttgattcctc caccgtcaat
      481 agaaagaatc cgtatttttt ttgccatatg ctattcgatt tatgatttct gattacacaa
      541 tattattaat caagcccttt tctcaaaaac atttcatgca tatatcctcc gaataatacc
      601 gtagtaaata aaaagaagcc cgagcagaat ttgtaaatgt ggaaatgttt gtgcagacag
      661 gtatcattca aataatttca tacacaaata taatcatgat ttgaaaaaaa tgcaaacagt
      721 ggaaaatatt tttcaaaact tcctgcgcta taggttgcct gataaaaaaa tcaggataag
      781 cgttgttcca ggtgtttttt ggaaatgtat tttaccggat accaggcggc aataaagccg
      841 ataatacata ctatcagaaa cacataaaca aaatcgagtg ccagtatccg aatgggataa
      901 gcatccatca caaaggtatc gccggtagaa atggtaataa ctccaaaggt ttgctgtatc
      961 caacacacca ccccgccaag aaccagcccc agcaaagccc ccgataaaga aatcatcatg
     1021 ccctcggtaa agaaaatgtt ccgtatagtt tggttgccgg cgccaaggct tttaagcaca
     1081 gcaatatctt tgcgcttatc gatgatgagc atgctcaggg agccaataac gttaaatgta
     1141 gcaatcagca ggatgaatga aaggtgcgca gcagtacttc ggggattccc ggccgc
//
LOCUS       DAOSFP010000469         1172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_128647_length_1172_cov_2.960609, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000469 DAOSFP010000000
VERSION     DAOSFP010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128647_length_1172_cov_2.960609"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PLI16_10650"
     CDS             <1..446
                     /locus_tag="PLI16_10650"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOH85060.1"
                     /translation="ALKTIKGIGKVKSEIYGPEIISMILDFCRDNDIVPEQIEIPVKI
                     KKQKINSKNVSLDLYKAGKSIVEIATERGFSTQTIEGHLAHFVGRGILDVKAFVDEEK
                     IKKVLTMLSADEKKSLGEIKHALGEEISYNELNFVVQHYKHLQEE"
     gene            650..>1172
                     /locus_tag="PLI16_10655"
     CDS             650..>1172
                     /locus_tag="PLI16_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOH85061.1"
                     /translation="MLKKIFLIIGCVSLLTGSHLVYGQKTTNYRSPIVQYRDAVELFN
                     KEKYGAAQENFKMLADKSDYPVEMRANALYYEALCALRLHNADAENLMMAFIKDYPEN
                     PKVNRGYYELADYYFTGKKYGSALKAYEKTDILQLNEEETADYYFKTGYSYFMQQDYA
                     NAKKSFFEIIDKES"
BASE COUNT          395 a          231 c          236 g          310 t
ORIGIN      
        1 aagcgctgaa aaccataaaa ggtatcggta aagtaaaatc agaaatttat ggccctgaaa
       61 ttatttctat gatactggat ttttgccgcg acaatgatat tgttccggaa cagattgaaa
      121 tacccgtcaa gattaaaaaa caaaaaatca actcaaaaaa tgttagtctc gacctgtaca
      181 aagcaggaaa aagcatcgtc gaaattgcta cggaacgagg attttcaacc cagaccattg
      241 aaggtcacct ggctcatttt gttggccgcg gcatcctgga tgtcaaagcc tttgtggacg
      301 aagaaaaaat caaaaaagtg ctgaccatgt taagcgctga cgaaaaaaaa tcgttgggcg
      361 aaatcaaaca tgccctgggc gaggaaattt cgtacaacga actgaatttt gtagtacagc
      421 attataagca tttgcaggaa gaataaaccg gcgtttccgt cattttatat ccgggtttta
      481 tcgttaaaac aagtcctttt tacttcaaag ttattaaact tccgctgttg ataattatgg
      541 catcccgccg gtttgccact ctttatttaa taataatttc gcagtacaaa ggataatagt
      601 atataagtgt ttaattatca acaggataca aattaaaaag tatgttgtta tgctgaaaaa
      661 aatctttctt atcatcggtt gtgtgagttt gctcacgggt tcacacctag tgtatggcca
      721 aaagacaacc aactatcggt cgcctatcgt tcaataccgc gatgccgttg agctgtttaa
      781 caaagaaaaa tacggtgctg ctcaagaaaa ctttaaaatg ctggccgaca aaagcgatta
      841 tccagtggag atgcgtgcca acgccctgta ttatgaggcg ctgtgtgcat tacgcctgca
      901 taatgccgat gctgaaaacc tgatgatggc attcatcaaa gattatcctg aaaaccccaa
      961 ggtaaaccgt ggctactatg agctggcaga ctattatttt acaggaaaaa aatacgggag
     1021 tgccctgaag gcctacgaaa aaaccgatat ccttcagctg aacgaagagg aaacagccga
     1081 ctattacttt aaaaccggct atagttactt tatgcagcag gattacgcca atgccaagaa
     1141 atcatttttt gaaatcatcg acaaagaatc ca
//
LOCUS       DAOSFP010000470         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_129668_length_1166_cov_3.822682, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000470 DAOSFP010000000
VERSION     DAOSFP010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129668_length_1166_cov_3.822682"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..267)
                     /locus_tag="PLI16_10660"
     CDS             complement(58..267)
                     /locus_tag="PLI16_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85062.1"
                     /translation="MKKEILFLLLLFYSLVSEVYAQQHVVFHINQPTQLIADAGPDSY
                     IPVGDSNVIGGMPAASGGSPAYTYL"
     gene            complement(394..1161)
                     /locus_tag="PLI16_10665"
     CDS             complement(394..1161)
                     /locus_tag="PLI16_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOH85063.1"
                     /translation="MTLPDVKKIIVVASGKGGVGKSTVSVNLAVALARQGFKVALVDA
                     DIYGPSIPKMLGMEELMPVVTQVDGKDIILPLEKYGVKVISIGFFVKNNQGLIWRGPM
                     AANAITQLFENTLWGETDYMIVDFPPGTGDIQLTTMQKMDLFGAIIVTTPQEIALNDA
                     RKAASMFTNQDLNVPIIGIIENMSWFIPEKHPGEQYFVFGKNGGAKIAAEIDAPLLGQ
                     IPLVMEVGEAAENGKSIYSQHNTAVIDAFEKIAERLI"
BASE COUNT          345 a          257 c          226 g          338 t
ORIGIN      
        1 caaaggagtc actattggat tgggcagggt actatcattt aggtagtcgc cgggagttca
       61 caggtaagta tatgcaggac tgccgcctga tgccgcaggc ataccgccaa taacatttga
      121 atcgccaaca gggatataac tatctggccc agcatcagcg attaattgtg tcggctgatt
      181 gatgtgaaaa accacatgtt gttgtgcata tacctcagaa acaaggctat aaaacaataa
      241 tagtaaaaaa agtatttctt ttttcataat atactgattg ggtaatacta aaatacgaaa
      301 ttttcaagaa ttaaataaat ttatgagaaa ataatacttc aatcaacagg ttgtttttcg
      361 caaataagat gaaacacaat atatcaactc gctttaaatc agtcgttcag ctatcttttc
      421 aaaggcatca atgacagccg tattatgttg actgtagata cttttaccat tttctgcggc
      481 ttcaccaacc tccataacca aagggatttg tccaagtagc ggggcatcga tttctgctgc
      541 tattttggct cctccatttt tcccaaaaac aaaatactgt tcgccgggat gtttttccgg
      601 aataaaccag gacatatttt caatgatacc tattatggga acattcaaat cctggttggt
      661 aaacatggaa gctgctttac gggcatcatt aagtgctatc tcctgcgggg tggtaacaat
      721 aattgcaccg aacaggtcca ttttctgcat ggtagtaagc tggatatcgc ctgtacctgg
      781 aggaaaatcc actatcatat aatccgtttc gccccataat gtattttcaa aaagctgggt
      841 aatggcattg gctgccatag gtccccgcca aatcaatccc tggttatttt taacaaaaaa
      901 gccaatggaa atcactttaa cgccatattt ttcaaggggc aggattatat cttttccgtc
      961 gacctgagtt accacgggca taagctcttc catccctaac atcttcggga tggatgggcc
     1021 gtaaatatca gcatccacca gagccacctt aaaaccctgc cttgccagag ccacggccag
     1081 gttgaccgac acggtagatt ttcctacgcc tcccttgcct gaagccacca caatgatttt
     1141 ttttacatcg ggcagtgtaa tttttt
//
LOCUS       DAOSFP010000471         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_135691_length_1132_cov_4.081708, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000471 DAOSFP010000000
VERSION     DAOSFP010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135691_length_1132_cov_4.081708"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..871
                     /locus_tag="PLI16_10670"
     CDS             149..871
                     /locus_tag="PLI16_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOH85064.1"
                     /translation="MKFGKWIGGGLGWAFGGPIGAILGFVFGSIVDNITVTTINTTHR
                     ASTQQGDFVASLLVLTAAVMKAEGQVLRSELNYVKRTFLQQFSEEETRQHMLVLRDIL
                     KQDIPVYDVCIQISQNMDYSSRLQLLHYLFGISQADNFSHPAEIRVIETIASYMRIAP
                     ADVNSIKAMFVKDTDSAYKILEVDPSVSDEELKKAYRRMAIKYHPDKVSHLGPEMQKA
                     ANEKFQKVNAAYEEIKKLRGIS"
     gene            complement(903..>1132)
                     /locus_tag="PLI16_10675"
     CDS             complement(903..>1132)
                     /locus_tag="PLI16_10675"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOH85065.1"
                     /translation="FIWQFTQHKMKVNKCKTLADIPAKSPESDAMSAELKKRGFSFVG
                     STICYAFMQAAGMVNDHLTSCFRYKEVQTKA"
BASE COUNT          331 a          221 c          269 g          311 t
ORIGIN      
        1 gaataatgaa atcaacatta ccggggtgca cattcccgtg attcccgaaa tatacaaacc
       61 tattctcgac aatcttgaaa agttaaacat caaaatggtt gaagaatatg gcctcccgtt
      121 aacagaggaa ttataaaatt gccaaaggat gaaatttggc aaatggattg gcggaggttt
      181 gggctgggct tttggtgggc ctattggtgc tatattggga tttgtctttg ggtcgattgt
      241 agataatatc acggttacca ccatcaatac tacccatcgg gcatcaacac agcagggaga
      301 ttttgtggcc agtctgctgg tattaacagc agcggtgatg aaagccgaag gacaagtcct
      361 gaggtcggaa ttaaattatg tgaaacgtac ttttttgcaa caattttccg aagaagaaac
      421 ccgtcagcac atgttggtgt tgcgcgatat tctaaagcag gatattcctg tatatgatgt
      481 gtgcatccag atttcgcaaa atatggatta ctcgtcgcgg ctgcagttgc tgcactattt
      541 gttcgggatt tcacaggccg ataatttttc gcatccagcc gaaatacgcg ttatcgaaac
      601 catagcctcc tacatgagaa ttgccccggc ggatgtaaat tctatcaagg cgatgtttgt
      661 taaagatacc gacagcgcct acaaaattct ggaagtggat ccttctgtca gtgatgaaga
      721 actcaaaaaa gcataccgca ggatggcaat aaaatatcac cccgacaagg tgagtcacct
      781 ggggccggag atgcagaagg ccgcaaacga aaagttccag aaagtaaatg ccgcatacga
      841 ggaaataaaa aagttgagag gcatttcctg attggatggg cggtgaatgg ttatgcttat
      901 gcttatgctt ttgtttggac ttctttatac ctgaaacagg atgtcaaatg gtcgtttacc
      961 atgccggctg cctgcataaa ggcgtagcaa atagttgacc cgacaaacga aaaaccacgt
     1021 tttttcagtt ctgcgctcat agcatccgat tcagggcttt tggcagggat gtcagctaat
     1081 gttttacatt tattcacttt cattttgtgt tgagtgaatt gccatataaa ag
//
LOCUS       DAOSFP010000472         1114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_139080_length_1114_cov_3.298395, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000472 DAOSFP010000000
VERSION     DAOSFP010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139080_length_1114_cov_3.298395"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLI16_10680"
     CDS             complement(<1..618)
                     /locus_tag="PLI16_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85066.1"
                     /translation="MGKKSKKKFSADKLPGIPDGPKTRNVLTNTADSPKKVRLYAAIA
                     VLFTLLLYIPSLNNDFIVNWDDGGYIHEHELVHKISWDNFVTIFNPTTFYKGNYHPLT
                     TFFFAVEYSLVGEHALLYHINNLILHLLNVLLVFVFIRLLSKRATIAALVALLFGIHP
                     MHVESVAWISERKDVLYTFFFMLSLIQYFYYVTKEASRRTHYILVL"
BASE COUNT          373 a          219 c          219 g          303 t
ORIGIN      
        1 caaaacaagt atgtagtgtg ttcgccgcga tgcctctttg gtcacataat aaaaatattg
       61 aatcagcgat aacatgaaaa agaaggtgta caacacatcc ttacgttccg aaatccaggc
      121 cactgactcc acatgcatag gatgtatgcc aaacagcaac gccaccaaag ccgcaatcgt
      181 cgcgcgtttc gataacagcc ggataaacac aaataccagc agcacattca gcaaatgaag
      241 tatcaggttg ttgatatgat atagcagcgc atgttcgccc accaacgaat attcaacggc
      301 aaagaaaaag gtagtcaacg gatggtaatt gcctttgtag aaagtggtag gattaaaaat
      361 tgtaacgaaa ttgtcccaag aaattttatg aaccagctcg tgttcatgta tatagccgcc
      421 atcatcccag ttgacgataa aatcattatt tagcgaagga atataaagca gcaaagtaaa
      481 taaaaccgct atggcagcat aaagacggac ttttttgggg ctgtcagcag tattggtcag
      541 gacattcctg gttttcggtc catccggaat gccaggcaat ttatctgccg aaaatttttt
      601 tttacttttc tttcccataa ttgttaccag aaattttcac aaaagtaatg ttcaaaggta
      661 cttgcgaatg caaaataaaa aaatttatca gaacttttta agaagcaata aagaaatcaa
      721 gtattccggc atgatgatag cgggttttat taatagttaa atcttttgac gtagcaaaat
      781 taaatcaaga ctccccatat tactttactt caataggaat gacaaatatt tatctcagat
      841 tatgctttta catcccttat taataaagag ttttctgaat catagaccaa ttaaatgttt
      901 gcaaattgca caaatgacag ttaatagtgg tgcggtggcc cgtcaaaacg gcggggcggc
      961 gcaggcgtga atcagcagga cggtgaaaat cagcaaaggc agcccgcact accgggctgt
     1021 ttgtctgaaa tggcctgcat taactgatga tgaatacacg cactatttct tacccaaaaa
     1081 taattcaaac tgttgttggc ctttgatggc cttt
//
LOCUS       DAOSFP010000473         1111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_139694_length_1111_cov_2.451705, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000473 DAOSFP010000000
VERSION     DAOSFP010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1111
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139694_length_1111_cov_2.451705"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLI16_10685"
     CDS             <1..572
                     /locus_tag="PLI16_10685"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOH85067.1"
                     /translation="YPAKLKTEYHYFQKKYGLTPIDGHLWRFLRIRPNNFPTIRLAQF
                     ASLIHRSENLFSKIIESNTLSDIYHLFDLSCSTYWHNHYVFDKVSESSSKKPVKSTIN
                     LLIINTIVPFIFAYGKTKNDENYTEKALDFLAALPPEKNRYTERYTATGIIPQNAMQS
                     QAMIQMFNDYCQPKKCLHCSIGDAILKQG"
     gene            complement(697..>1111)
                     /locus_tag="PLI16_10690"
     CDS             complement(697..>1111)
                     /locus_tag="PLI16_10690"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOH85068.1"
                     /translation="INENLNNNFISLLVINSFVNPNTGIGSATSAGVSLLGKSASEVL
                     SNQLSNWLSQISKNVNIGINYRPGDNISQEEVAVALSTQLFNDRVTVSSNLGVATGQT
                     ATGENKNSNQIVGDVDIEVKISKALKLKVYNHTNQ"
BASE COUNT          322 a          251 c          207 g          331 t
ORIGIN      
        1 agtaccctgc caaattaaaa accgaatacc attactttca gaaaaaatat ggattaaccc
       61 cgatagatgg acatttgtgg agatttctcc gcatccggcc caacaatttc cctaccatca
      121 ggctggcaca gtttgccagc ctgatacacc ggtccgaaaa cctgttcagc aaaataattg
      181 aaagcaacac attgtcagat atttatcatc tgttcgactt gagctgctcc acatattggc
      241 acaatcatta tgtgtttgat aaagtcagtg aatcttcctc gaagaagccg gtgaaaagca
      301 ccatcaacct attaataatc aacactatag ttccattcat ttttgcctat ggcaaaacaa
      361 aaaatgacga aaactatacc gaaaaagctt tggattttct tgccgcctta ccacccgaga
      421 aaaaccgcta tacggaacgc tatactgcaa caggcattat cccacaaaat gccatgcagt
      481 cgcaggccat gatacaaatg tttaatgatt actgccagcc aaaaaaatgc ctgcattgca
      541 gcataggcga tgctattttg aaacagggct gaacaggcgg caggattact ttttcctgtt
      601 tttgtttttc aggaataaat cccggtaggt attaaactct ttgcggtata cgataccaat
      661 tccctgtgta taaggtgctg tatattgcaa aatactttac tggtttgtat ggttgtacac
      721 ctttagcttt aatgccttgg aaattttcac ttcaatatcc acatcgccta ctatctgatt
      781 cgaatttttg ttttcacctg ttgctgtctg ccctgtagca acgccaaggt tgctggatac
      841 ggttacccgg tcattaaaaa gttgtgtcga aagcgccacc gccacttctt cctgcgaaat
      901 attgtcgcct ggccggtaat taataccgat attgacattt ttgctgattt gcgaaagcca
      961 gttgcttaat tggtttgaaa gtacttcgct ggcacttttt cccaacaggc ttacccctgc
     1021 cgaagtggcc gaacctatac ctgtatttgg attaacaaaa ctgttgataa ccagcaggga
     1081 tataaagtta ttgttgagat tttcgtttat a
//
LOCUS       DAOSFP010000474         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_151462_length_1055_cov_4.108000, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000474 DAOSFP010000000
VERSION     DAOSFP010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151462_length_1055_cov_4.108000"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLI16_10695"
     CDS             <1..750
                     /locus_tag="PLI16_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85069.1"
                     /translation="VISQALNSTTAQINLLDTYGKPTETNVGMTLYDNFSKTIKYDFV
                     HTFNNEGLPDTLMLDPLLAYDLVVHTIPPVSHDSIVITPGKHTTIAANTPQGTLELKV
                     GGKVPPNLTFQCIVRQSGKMETLNVQSFTQKTKYLTGIYDLEVLSLPRLKINDVEIKQ
                     SYNTTVEIPTPGTAIIQIAMRGYGDLYTEENNKLTWIYKLNEEGQQEMLYLLPGKYRI
                     IFRSKYATHSFYTIDKPFKIESGITTRVNFY"
     gene            848..>1055
                     /locus_tag="PLI16_10700"
     CDS             848..>1055
                     /locus_tag="PLI16_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOH85070.1"
                     /translation="MNRFQKTGDKDTRSGFGEALNELGKENPQVIALVADLTGSLKMD
                     AFAKNYPERFIQVGIAEANMMGIAA"
BASE COUNT          341 a          223 c          226 g          265 t
ORIGIN      
        1 gtaatttcac aggcgctcaa ttccaccacc gcgcagatta atttgctgga tacctatggt
       61 aaaccaaccg aaaccaatgt cggtatgacg ttgtatgata atttctcgaa aaccataaag
      121 tatgattttg tgcatacctt taataatgaa ggtttgccgg ataccctgat gctggatccg
      181 ctgctggcat acgacctggt ggttcatacc attccgccgg tttcccacga cagcattgtg
      241 attaccccgg ggaaacatac caccatcgct gccaatacgc cacagggaac ccttgaactt
      301 aaagtgggag ggaaggtgcc gcctaatctc acttttcaat gtattgtaag gcaaagcggg
      361 aaaatggaaa ccctcaacgt gcagtcgttt acccaaaaaa caaaatatct taccggcatt
      421 tatgatctgg aggtgcttag tttgccgcgg ctgaaaatca acgatgtgga aatcaaacag
      481 agttataata ccaccgttga aatacccact cccggcacgg ccattattca gattgccatg
      541 cggggatacg gcgacttgta cactgaagaa aacaacaaat tgacatggat atataaattg
      601 aatgaagaag gtcagcagga aatgttgtac ctgttgcccg gcaaataccg gattattttc
      661 cgatcgaagt atgccacgca ttctttttat actatcgata aaccatttaa aattgaatca
      721 ggtatcacaa caagagttaa cttttattaa tatagaatat atttcagcaa aagacgcagt
      781 tttactgata gttttctgcg aaaatcagcg agatctgcgg gaaatattaa aatcataaaa
      841 aaaacaaatg aatagatttc aaaaaacagg agacaaagac acccgctcgg gttttggtga
      901 agcgctgaac gaattaggca aagaaaaccc acaagttata gccctggtgg ccgatttaac
      961 gggcagcctg aaaatggatg ctttcgccaa gaattacccg gaacgtttta tacaggtcgg
     1021 tattgctgaa gccaatatga tgggtatagc cgcag
//
LOCUS       DAOSFP010000475         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_1776
            NODE_152277_length_1052_cov_2.334002, whole genome shotgun
            sequence.
ACCESSION   DAOSFP010000475 DAOSFP010000000
VERSION     DAOSFP010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863305
            Sequence Read Archive: SRR4236666
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:37:31
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,141
            CDSs (total)                      :: 2,119
            Genes (coding)                    :: 2,103
            CDSs (with protein)               :: 2,103
            Genes (RNA)                       :: 22
            tRNAs                             :: 21
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 5 of 16
            Pseudo Genes (incomplete)         :: 11 of 16
            Pseudo Genes (internal stop)      :: 2 of 16
            Pseudo Genes (multiple problems)  :: 2 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152277_length_1052_cov_2.334002"
                     /isolate="AMR_MDS_1776"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="39.88 N 75.22 W"
                     /collection_date="2015-04-21"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLI16_10705"
     CDS             <1..845
                     /locus_tag="PLI16_10705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOH85071.1"
                     /translation="RTKGYSWMQTISGFFGVFAYVFGAIFGNYALIYVGAGITFFFSI
                     VPTLFIKEPRSLTEAPATTADTPALKDSKKINRSGLMKIYIAHAFTWLGVQTMFVYIF
                     AFISQFKFSITDTSLLEQSQKDEIGQIIAIAFAILNTVGFILPAPVLEPLTRKIGRVR
                     THIACIFIMALGYWGIVFLGHSAIVLYLLMAVAGIGWASVVSLPFAIMTEMVDKTKMG
                     LFMGIFNLSVVIPQLVVSLFLGSFIQAADNKVLIFVISAVSLTISGLLWMLVKERKEE
                     SAEK"
BASE COUNT          260 a          225 c          243 g          324 t
ORIGIN      
        1 cacgcaccaa gggctatagc tggatgcaga cgatttccgg attcttcggc gttttcgcgt
       61 atgtgtttgg cgctattttc ggcaattatg cactgattta tgtgggcgca ggcatcacat
      121 ttttcttttc gatagtccct accctcttta tcaaagagcc acgcagcctg acagaagcac
      181 ccgccaccac cgctgacacc ccggcgttga aagactcaaa aaaaataaac cgcagcggcc
      241 tgatgaagat ttatattgcc catgccttta cctggcttgg ggtgcagacc atgtttgtgt
      301 acatttttgc ttttatcagc cagtttaaat tcagcattac cgacacttcc cttttggaac
      361 aaagccagaa agatgaaata ggacaaatta tcgccatcgc cttcgcgata ttaaacactg
      421 tgggatttat tttgccggcc ccggtcctcg agccgcttac cagaaagata ggcagggtgc
      481 gtacgcatat tgcctgtatt tttatcatgg ctttgggtta ttggggcata gtatttttag
      541 gacactcagc tattgttttg tacctgctga tggctgtggc cggcattggc tgggcttcgg
      601 tggtgagcct gccttttgcc attatgaccg aaatggtgga caagacaaag atgggacttt
      661 ttatgggtat cttcaacttg tcggtggtga taccgcaact ggtggtgagt ttgtttttgg
      721 gcagttttat acaggctgcc gacaacaagg tgctgatttt tgtcatcagt gccgtatcgc
      781 tcactatatc gggcctgtta tggatgctgg tgaaagagag gaaagaggaa agtgctgaga
      841 aatgattgaa tttttttaac cgcaaagaat cgttattgtc cttcaaaaaa atcttcgtct
      901 aactctttta cctcatactt tgttattatc tgaacgccga cattgtattg atgcatcaga
      961 cccaatataa ctaaaccata gcagtcactg tttgccgaaa taaagtttaa ggtcgattta
     1021 ctatagtcct aattctttgt atatcatgga tg
//
